As text, or see rules and steps
# Pyruvate is a central metabolite, so GapMind represents transport only. # actP-yjcH is usually annotated as acetate permease and associated protein, but they are # often involved in pyruvate transport instead. # A group of heterodimeric pyruvate transporters. This is the actP-like large subunit. # WCS417:GFF1743 = A0A1N7U9G2, # azobra:AZOBR_RS02940 = G8AHY6, # pseudo1_N1B4:Pf1N1B4_3673 = A0A166PH03, # pseudo6_N2E2:Pf6N2E2_5849 = A0A165ZRB0, # and psRCH2:Psest_0347 (GFF346) # Closely related to E. coli actP = P32705, so ignore that. # Added Marinobacter HP15_1251 (E4PHQ0) because it has a specific phenotype (if mild) and fills the gap. # And it is closely related to the D-alanine uptake system , so ignore that. actP large subunit of pyruvate transporter (actP-like) uniprot:A0A1N7U9G2 uniprot:G8AHY6 uniprot:A0A166PH03 uniprot:A0A165ZRB0 ignore:SwissProt::P32705 uniprot:E4PHQ0 curated:reanno::psRCH2:GFF346 # This is the yjcH-like small subunit. # These are similar to E. coli b4068/yjcH/18096, involved in glycolate & acetate transport, so ignore that. # Added Marinobacter HP15_1252 (E4PHQ1) because it has a specific phenotype (if mild) and fills the gap. yjcH putative small subunit of pyruvate transporter (yjcH-like) curated:reanno::WCS417:GFF1742 curated:reanno::azobra:AZOBR_RS02935 curated:reanno::pseudo1_N1B4:Pf1N1B4_3672 curated:reanno::pseudo6_N2E2:Pf6N2E2_5848 ignore:reanno::Keio:18096 uniprot:E4PHQ1 curated:reanno::psRCH2:GFF345 # Most of these transporters were identified using curated clusters and # query: transporter:pyruvate. ActP-yjcH and cstA-ybdD were identified from mutant fitness data. pyruvate-transport: yjcH actP # E. coli cstA (P15078) was originally thought to be a peptide transporter, but # is involved in pyruvate transport along with ybdD (PMID:29358499). # The related protein btsT (P39396, formerly yjiY) is also a pyruvate transporter. # And a similar protein from Cupriavidus basilensis, RR42_RS20515 = A0A0C4Y7X7, is important for pyruvate utilization. # As is AO353_25255 (A0A0N9VZ52) from Pseudomonas fluorescens FW300-N2E3. # CstA and RR42_RS20515 seem to operate together with ybdD or similar proteins, but purified BtsT is reported to # transport pyruvate (PMID:29061664), so it may not require a ybdD-like component. # It is not clear if AO353_25255 functions with a ybdD-like component: the downstream gene, AO353_25260, # is ybdD-like and has subtle defects during growth on pyruvate. # Q0P9Y2 (CSTA_CAMJE) was proposed to be a peptide transporter in Campylobacter jejuni, but # is suspiciously similar to pyruvate transporters, so is ignored. cstA large subunit of pyruvate transporter (CstA) uniprot:A0A0C4Y7X7 curated:SwissProt::P15078 curated:SwissProt::P39396 uniprot:A0A0N9VZ52 ignore:SwissProt::Q0P9Y2 # E. coli ybdD (P0AAS9) is involved in pyruvate transport with cstA (PMID:29358499). # And a similar protein from Cupriavidus basilensis, RR42_RS20510, is important for pyruvate utilization. ybdD small subunit of pyruvate transporter (YbdD) uniprot:P0AAS9 curated:reanno::Cup4G11:RR42_RS20510 pyruvate-transport: cstA ybdD SLC5A8 sodium-coupled pyruvate transporter curated:SwissProt::Q3ZMH1 curated:SwissProt::Q7T384 curated:SwissProt::Q8N695 pyruvate-transport: SLC5A8 # DctP from Nostoc, Rhodobacter; associated with dctQ and dctM. # Similar to TakP (Q3J1R2), which is misannotated and not characterized. # The related protein Q48AL6 = CPS_0129 was shown to bind calcium-pyruvate (PMC4310620). dctP pyruvate TRAP transporter, substrate-binding component curated:SwissProt::Q8YSQ6 curated:TCDB::D5ALT6 ignore:SwissProt::Q3J1R2 uniprot:Q48AL6 dctQ pyruvate TRAP transporter, small permease component curated:TCDB::D5ATK0 curated:TCDB::Q8YSQ8 dctM pyruvate TRAP transporter, large permease component curated:TCDB::D5ATK1 curated:TCDB::Q8YSQ7 pyruvate-transport: dctM dctP dctQ JEN1 pyruvate symporter JEN1 curated:CharProtDB::CH_091331 curated:CharProtDB::CH_123467 pyruvate-transport: JEN1 # formerly yjiY; cstA-like. # E. coli cstA (uniprot:P15078) might be a pyruvate transporter, so ignore btsT pyruvate symporter BtsT curated:SwissProt::P39396 ignore:SwissProt::P15078 pyruvate-transport: btsT # Note this is related to actP, and was identified by genetics, and is adjacent # to a small protein (cg0952) that could be the other subunit (it belongs to DUF485) mctC pyruvate symporter MctC curated:SwissProt::Q8NS49 pyruvate-transport: mctC MCT4 pyruvate symporter MCT4 (SLC16A3) curated:TCDB::O15427 pyruvate-transport: MCT4 # TCDB and SwissProt have slightly different sequences, include both mctP pyruvate permease MctP curated:TCDB::Q8VM88 curated:SwissProt::Q1M7A2 pyruvate-transport: mctP # Ignore various mitochondrial carriers and chloroplast transporters # This has weak evidence to be a pyruvate transporter, so ignore: # Acetate/haloacid transporter, Dehp2, with a possible atypical topology curated:TCDB::F8SVK1 all: pyruvate-transport
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory