As text, or see rules and steps
# Rhamnose utilization in GapMind is based on MetaCyc pathway # I via L-rhamnulose 1-phosphate aldolase (metacyc:RHAMCAT-PWY), # pathway II via 2-keto-3-deoxy-L-rhamnonate aldolase (metacyc:PWY-6713), # and pathway III via 2,4-diketo-3-deoxyrhamnonate hydrolase (metacyc:PWY-6714). # Fitness data shows that the (distant) homolog in Bacteroides thetaiotaomicron (BT3765, Q8A1A1) is also # a rhamnose transporter rhaT L-rhamnose:H+ symporter RhaT curated:SwissProt::P27125 uniprot:Q8A1A1 # Transporters were identified usiung: # query: transporter:L-rhamnose:rhamnose:L-rhamnofuranose:L-rhamnopyranose:CPD-16564:CPD-16565:CPD0-1112:CPD-15405 rhamnose-transport: rhaT # 4-part ABC transporter rhaPQST. # In TCDB, the rhamnose transporter of Rhizobium leguminosarum is described as rhaSTP # but rhaQ (Q7BSH2_RHILT) is also probably part of this system. # The comment in TCDB also speculates about group translocation, because the R. leguminosarum # system requires the rhamnose/rhamnulose kinase rhaK for activity; but # in Sinorhizobium meliloti, which has a similar system, # the rhaK protein has rhamnulokinase activity only # (Rivers 2015, URL:https://mspace.lib.umanitoba.ca/handle/1993/30289). rhaP L-rhamnose ABC transporter, permease component 1 (RhaP) curated:TCDB::Q7BSH3 rhaQ L-rhamnose ABC transporter, permease component 2 (RhaQ) uniprot:Q7BSH2 rhaS L-rhamnose ABC transporter, substrate-binding component RhaS curated:TCDB::Q7BSH5 # Because of the one-component transporter rhaT, the ATPase component is named rhaT' in GapMind. rhaT' L-rhamnose ABC transporter, ATPase component RhaT curated:TCDB::Q7BSH4 rhamnose-transport: rhaP rhaQ rhaS rhaT' # Fitness data suggests Echvi_1617 is the rhamnose transporter. # It is probably Na+ dependent, but this is uncertain. Echvi_1617 L-rhamnose transporter uniprot:L0FX46 rhamnose-transport: Echvi_1617 # In Burkholderia phytofirmans PsJN, a 3-part ABC transporter is involved # in utilization of L-rhamnose, L-fucose, and xylitol BPHYT_RS34250 L-rhamnose ABC transporter, substrate-binding component uniprot:B2T9W0 BPHYT_RS34245 L-rhamnose ABC transporter, ATPase component uniprot:B2T9V9 BPHYT_RS34240 L-rhamnose ABC transporter, permease component uniprot:B2T9V8 rhamnose-transport: BPHYT_RS34250 BPHYT_RS34245 BPHYT_RS34240 rhaM L-rhamnose mutarotase EC:5.1.3.32 rhaA L-rhamnose isomerase EC:5.3.1.14 rhaB L-rhamnulokinase EC:2.7.1.5 # BT3766 (Q8A1A0) was confirmed by fitness data rhaD rhamnulose 1-phosphate aldolase EC:4.1.2.19 uniprot:Q8A1A0 import fructose.steps:tpi # triose-phsophate isomerase # The EC number is for the NAD dependent reaction. # There's also a NADP dependent reaction, sometimes given this EC, sometimes not. aldA lactaldehyde dehydrogenase EC:1.2.1.22 ignore_other:lactaldehyde dehydrogenase # BT3767 (Q8A199) was confirmed by fitness data fucO L-lactaldehyde reductase EC:1.1.1.77 uniprot:Q8A199 # Lactaldehyde might be oxidized to # lactate and secreted (or oxidized to pyruvate); or, it might be # reduced to propane-1,2-diol and secreted. lactaldehyde-conversion: aldA lactaldehyde-conversion: fucO # In pathway I, the mutarotase rhaM forms # beta-rhamnopyranose, isomerase rhaA forms rhamnulofuranose, kinase rhaB # forms rhamnulose 1-phosphate, aldolase rhaD forms (S)-lactaldehyde and # glycerone phosphate, and tpi converts glycerone phosphate to # glyceraldehyde 3-phosphate. all: rhamnose-transport rhaM rhaA rhaB rhaD tpi lactaldehyde-conversion # The rhamnofuranose dehydrogenase may be either NADH or NADPH dependent, or use either LRA1 L-rhamnofuranose dehydrogenase EC:1.1.1.378 EC:1.1.1.173 EC:1.1.1.377 LRA2 L-rhamnono-gamma-lactonase EC:3.1.1.65 # Ignore BPHYT_RS34235, a putative accessory domain LRA3 L-rhamnonate dehydratase EC:4.2.1.90 ignore:reanno::BFirm:BPHYT_RS34235 LRA4 2-keto-3-deoxy-L-rhamnonate aldolase EC:4.1.2.53 # In pathway II, the 1-dehydrogenase LRA1 forms L-rhamnono-1,4-lactone, # the lactonase LRA2 forms L-rhamnonate, the dehydratase LRA3 forms # 2-dehydro-3-deoxy-L-rhamnonate, and the aldolase LRA4 forms pyruvate and # lactaldehyde. all: rhamnose-transport LRA1 LRA2 LRA3 LRA4 lactaldehyde-conversion # The enzyme from Sphingomonas strain SKA58 is Q1NEI6 not Q1NEI7; there is an error in MetaCyc. # Q1NEI6 is annotated correctly in other resources LRA5 2-keto-3-deoxy-L-rhamnonate 4-dehydrogenase EC:1.1.1.401 ignore:metacyc::MONOMER-16233 # EC:3.7.1.26 has been assigned but not linked to the characterized protein, which is # EAT09363.1 or metacyc:MONOMER-16233 (PMID:19187228) -- but MONOMER-16233 is misannotated as # a dehydrogenase. # And, ignore uniprot:Q39BA7, which is very similar to LRA6 from Burkholderia phytofirmans PsJN # but is reported to be a ureidoglycolate lyase (PMID:14506266). LRA6 2,4-diketo-3-deoxyrhamnonate hydrolase curated:metacyc::MONOMER-16233 term:L-2,4-diketo-3-deoxyrhamnonate hydrolase term:2,4-diketo-3-deoxy-L-rhamnonate hydrolase EC:3.7.1.26 ignore:SwissProt::Q39BA7 # In pathway III, rhamnose is also oxidized and dehydrated to # 2-dehydro-3-deoxy-L-rhamnonate, but then, dehydrogenase LRA5 forms # 2,4-didehydro-3-deoxy-L-rhamnonate and hydrolase LRA6 forms L-lactate # and pyruvate. all: rhamnose-transport LRA1 LRA2 LRA3 LRA5 LRA6
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory