Family Search for PF20247 (DUF6602)

PF20247 hits 9 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

NUCC_VIBMT / P0DUD6 CRISPR-associated endodeoxyribonuclease NucC; EC 3.1.-.- from Vibrio metoecus (see paper)
Aligns to 23:128 / 245 (43.3%), covers 98.1% of PF20247, 112.4 bits

• function: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).
  function: A cyclic nucleotide-activated dsDNase. In the presence of 3',3',3'-cyclic AMP-AMP-AMP (cAAA), and to a much lesser extent 3',3',3'-cyclic AMP-AMP-GMP (cAAG) and cyclic-di-AMP (c-di-AMP), endonucleolytically degrades dsDNA. The nuclease digests dsDNA to about 50 bp lengths. Not stimulated by cGAMP or linear di-AMP (PubMed:31932164). DNA has been modeled to contact a pair of juxtaposed active sites (one from each layer of the hexamer), accounting for cleavage on both strands (By similarity).
  cofactor: Mg(2+)
  subunit: Forms homotrimers and homohexamers; in the presence of cAAA only the homohexamers of face-to-face associated trimers are formed (PubMed:31932164). The 2 cAAA-binding sites are on the exterior of the hexamer at the three-way junction, there are maximally 2 cyclic nucleotides per hexamer (Probable).

NUCC_PSEAI / P0DTF8 Endodeoxyribonuclease NucC; NucC nuclease; EC 3.1.-.- from Pseudomonas aeruginosa (see 2 papers)
Aligns to 22:127 / 241 (44.0%), covers 97.1% of PF20247, 106.6 bits

• function: Effector DNase of a CBASS antivirus system (Probable) (PubMed:31932164, PubMed:31932165). CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type III-C(AAA) CBASS system (PubMed:32839535).
  function: A cyclic nucleotide-activated dsDNase. In the presence of 3',3',3'-cyclic AMP-AMP-AMP (cAAA) and to a lesser extent cyclic-di-AMP (c-di-AMP), endonucleolytically degrades dsDNA (Probable). Binds one cAAA in a pocket on one surface of the trimer; cAAA binding promotes hexamerization which is probably necessary for nuclease activation (PubMed:31932164). The nuclease digests dsDNA to about 50 bp lengths. DNA has been modeled to contact a pair of juxtaposed active sites (one from each layer of the hexamer), accounting for cleavage on both strands (By similarity).
  cofactor: Mg(2+)
  subunit: Self-oligomerizes (PubMed:31932165). Forms homotrimers; in the presence of cAAA the trimers associate face-to-face to form homohexamers. The 2 cAAA-binding sites are on the exterior of the hexamer at the three-way junction, there are maximally 2 cyclic nucleotides per hexamer (PubMed:31932164).

NUCC_ECOM1 / D7Y2H5 Endodeoxyribonuclease NucC; NucC nuclease; EC 3.1.-.- from Escherichia coli (strain MS 115-1) (see 2 papers)
Aligns to 22:127 / 241 (44.0%), covers 98.1% of PF20247, 104.5 bits

• function: Effector DNase of a CBASS antivirus system (PubMed:31932164, PubMed:31932165). CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type III-C(AAA) CBASS system (PubMed:32839535).
  function: A cyclic nucleotide-activated dsDNase. In the presence of 3',3',3'-cyclic AMP-AMP-AMP (cAAA), and to a lesser extent 3',3',3'- cyclic AMP-AMP-GMP (cAAG) and cyclic-di-AMP (c-di-AMP), endonucleolytically degrades dsDNA (PubMed:31932164, PubMed:31932165). Binds one cAAA in a pocket on one surface of the trimer; cAAA binding promotes hexamerization, which is necessary for nuclease activation. Also binds c-diAMP or linear di-AMP with lower affinity. The nuclease digests dsDNA to about 50 bp lengths with a 2-base 3' overhang and a consensus recognition site of 5'-Axx|T-3'. DNA has been modeled to contact a pair of juxtaposed active sites (one from each layer of the hexamer), accounting for cleavage on both strands and the 2-base overhang (PubMed:31932164).
  function: Protects E.coli strain JP313 against bacteriophage lambda cI- infection. When the cdnC-cap7-cap6-nucC operon is transformed into a susceptible strain it confers bacteriophage immunity. Mutations in the sensor (Cap7 also called HORMA) or effector proteins (CdnC, NucC) but not the disassembly protein (Cap6 also called Trip13) no longer confer immunity. The presence of the intact operon leads to culture collapse and cell death which occurs before the phage has finished its replication cycle, thus protecting non-infected bacteria by aborting the phage infection and preventing its propagation.
  cofactor: Mg(2+)
  subunit: Self-oligomerizes (PubMed:31932165). Forms homotrimers; in the presence of cAAA the trimers associate face-to-face to form homohexamers. The 2 cAAA-binding sites are on the exterior of the hexamer at the three-way junction, there are maximally 2 cyclic nucleotides per hexamer (PubMed:31932164).

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