Family Search for PF01817 (CM_2)
PF01817 hits 149 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
VP0547 chorismate mutase/prephenate dehydrogenase from Vibrio parahaemolyticus RIMD 2210633
Aligns to 9:87 / 375 (21.1%), covers 98.7% of PF01817, 87.8 bits
Q02287 T-protein from Enterobacter agglomerans
Aligns to 9:87 / 373 (21.2%), covers 98.7% of PF01817, 82.9 bits
GSU1828 chorismate mutase domain protein from Geobacter sulfurreducens PCA
Aligns to 7:84 / 103 (75.7%), covers 98.7% of PF01817, 82.1 bits
Q0PBJ3 Bifunctional chorismate mutase/prephenate dehydratase from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Aligns to 8:85 / 357 (21.8%), covers 98.7% of PF01817, 81.8 bits
- Novel Drug Targets for Food-Borne Pathogen Campylobacter jejuni: An Integrated Subtractive Genomics and Comparative Metabolic Pathway Study
Mehla, Omics : a journal of integrative biology 2015 - “...Q0PAL6 Q0PAZ1 Q0P9X4 Q9PJ53 Q0PC20 Q9PIK3 Q9PIK2 Q9PIK1 Q0PBJ3 Q0PBA5 Q9PI11 P0C632 P0C630 Q9PNT2 Q9PMV3 Q0P8N9 Q9PM41 Q9PIT2 Q0PAS0 Q0PB07 Q9PIM1 Q9PHU0 Q9PIZ5...”
- “...14 Q9PIK3 15 Q9PIK2 16 Q9PIK1 17 Q0PBJ3 Drug name DB00229, DB00267, DB00301, Cefotiam, Cefmenoxime, Flucloxacillin, Penicillin V, DB00417, DB00456, DB00713,...”
TyrA / b2600 fused chorismate mutase/prephenate dehydrogenase (EC 5.4.99.5; EC 1.3.1.12) from Escherichia coli K-12 substr. MG1655 (see 4 papers)
tyrA / P07023 fused chorismate mutase/prephenate dehydrogenase (EC 5.4.99.5; EC 1.3.1.12) from Escherichia coli (strain K12) (see 12 papers)
P07023 T-protein from Escherichia coli (strain K12)
b2600 fused chorismate mutase T/prephenate dehydrogenase from Escherichia coli str. K-12 substr. MG1655
Aligns to 9:87 / 373 (21.2%), covers 98.7% of PF01817, 81.5 bits
- Identification of enzymes and regulatory proteins in Escherichia coli that are oxidized under nitrogen, carbon, or phosphate starvation
Noda, Proceedings of the National Academy of Sciences of the United States of America 2007 - “...P0A9P1 P0A6M9 P0A6Q0 Q59346 P23847 P0A7A0 P23721 P0AD61 P07023 Protein name Protein MW Protein PI Dihydrolipoyl dehydrogenase, 3 Elongation factor G (EF-G), 2...”
- Analysis of the pmsCEAB gene cluster involved in biosynthesis of salicylic acid and the siderophore pseudomonine in the biocontrol strain Pseudomonas fluorescens WCS374
Mercado-Blanco, Journal of bacteriology 2001 - “...influenzae (P43902), Erwinia herbicola (Q02287), and E. coli (P07023). The homology was located in the N-terminal domain of TyrA. TyrA proteins are larger than...”
- Ferric Citrate Uptake Is a Virulence Factor in Uropathogenic Escherichia coli
Frick-Cheng, mBio 2022 - “...sufE Cysteine desulfuration protein 3.8 b1679 sufS Selenocysteine lyase 4.1 b1680 tyrA Chorismate mutase 3.7 b2600 ybdZ Enterobactin biosynthesis protein 6.1 b4511 ybgS Uncharacterized protein 4.3 b0753 ybiX PKHD-type hydroxylase 3.7 b0804 yciG Uncharacterized protein 4.5 b1259 yddM Putative DNA-binding transcriptional regulator 4.6 b1477 ydiE Uncharacterized...”
- Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions
Orth, BMC systems biology 2012 - “...(b0073) 2.00E-15 R00732 (R) III aroA (b0908) 5.00E-32 murA (b3189) 7.00E-8 R00733 (R) III tyrA (b2600) 2.80E-2 R01393 (R) I global orphan R01618 (R) IV glgP (b3428) 2.10 R01713 (F) I global orphan R01731 (F) IV tyrB (b4054) * R01785 (R) III rhaD (b3902) * R01902...”
- More than just a metabolic regulator--elucidation and validation of new targets of PdhR in Escherichia coli
Göhler, BMC systems biology 2011 - “...0.977 2.124 b0628 lipA * -0.075 3.914 b1596 ynfM 0.927 0.007 b4052 dnaB -0.018 3.864 b2600 tyrA 0.915 2.065 b0085 murE $ 0.167 3.659 b2505 yfgH 0.820 0.425 b0822 ybiV 0.284 3.653 b0333 prpC 0.729 0.021 b2683 ygaH -0.077 3.628 b0331 prpB 0.713 0.024 b0436 tig...”
- Genome-scale analysis to the impact of gene deletion on the metabolism of E. coli: constraint-based simulation approach
Xu, BMC bioinformatics 2009 - “...b1260 b0914 b1098 b1136 b1261, b1262 s0001 b2827 b1263, b1264 b3648 b1693, b2329 s0001 b2599, b2600 b3389 SS AAM FM ACM HM CM genes b0928 b1415 b1415 b2019, b2020 b2750, b2751 b3941 b3608 b2021, b2022 b2752, b2762 b2023, b2024 b2763, b2764 b2025, b2026 b3607 SS IITM...”
- Experimental and computational assessment of conditionally essential genes in Escherichia coli
Joyce, Journal of bacteriology 2006 - “...(b1261) trpC (b1262) trpD (b1263) trpE (b1264) tyrA (b2600) Group 8262 JOYCE ET AL. J. BACTERIOL. two experimentally defined essential genes. We then examined...”
- Interfering with different steps of protein synthesis explored by transcriptional profiling of Escherichia coli K-12
Sabina, Journal of bacteriology 2003 - “...hdeB b3524 b2601 b1261 b1264 b1493 b3517 b3320 b1263 b2600 b1260 b1973 b1262 b3340 b1779 b3321 b3308 b3304 b3616 b3339 b2155 b3829 b3317 b3296 b2913 b0631 b2416...”
- “...b1264 b1493 b3517 b3774 b1289 b2553 b0698 b1004 b0179 b2600 b1260 b3509 b1261 b2342 b2800 b3460 b3458 b1263 b1783 b0907 b0461 b2464 b0812 b0903 b1262 b0126...”
ECs3463 chorismate mutase-T / prephenate dehydrogenase from Escherichia coli O157:H7 str. Sakai
Aligns to 9:87 / 373 (21.2%), covers 98.7% of PF01817, 81.5 bits
A0A140N544 T-protein from Escherichia coli (strain B / BL21-DE3)
Aligns to 9:87 / 373 (21.2%), covers 98.7% of PF01817, 81.5 bits
YP_0399 T-protein [includes: chorismate mutase and prephenate dehydrogenase] from Yersinia pestis biovar Medievalis str. 91001
Aligns to 9:87 / 373 (21.2%), covers 98.7% of PF01817, 81.3 bits
Gmet_1955 Chorismate mutase from Geobacter metallireducens GS-15
Aligns to 7:84 / 98 (79.6%), covers 98.7% of PF01817, 80.2 bits
O67085 Bifunctional chorismate mutase/prephenate dehydratase from Aquifex aeolicus (strain VF5)
Aligns to 8:89 / 362 (22.7%), covers 100.0% of PF01817, 79.9 bits
DET0461 chorismate mutase/prephenate dehydratase from Dehalococcoides ethenogenes 195
Aligns to 7:85 / 358 (22.1%), covers 98.7% of PF01817, 79.8 bits
Fisuc_2558 Chorismate mutase from Fibrobacter succinogenes subsp. succinogenes S85
Aligns to 7:87 / 87 (93.1%), covers 98.7% of PF01817, 79.3 bits
- Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85
Wu, Scientific reports 2017 - “...transcription regulators Fisuc_0335, Fisuc_0933 and Fisuc_1186, a diguanylate cyclase Fisuc_2957, and genes Fisuc_0137, Fisuc_0138 and Fisuc_2558, which are involved in tryptophan metabolism. A gene ontology enrichment analysis was performed on differentially expressed genes. Among the up-regulated genes in the pH 5.65 samples, category V: defense mechanisms...”
- “...Fisuc_2091 2.5 0.001303 Rubredoxin-type Fe(Cys)4 protein Fisuc_2123 2.3 0.002766 4Fe-4S ferredoxin iron-sulfur binding domain protein Fisuc_2558 1.9 2.99E-15 Chorismate mutase Fisuc_2559 2.0 1.03E-16 Prephenate dehydrogenase Fisuc_2908 2.1 0.000191 (Sulfur transfer protein involved in) thiamine biosynthesis protein ThiS Acid survival for E . coli knockout mutants To...”
P43902 prephenate dehydrogenase (EC 1.3.1.12) from Haemophilus influenzae (see paper)
HI1290 chorismate mutase / prephenate dehydrogenase (tyrA) from Haemophilus influenzae Rd KW20
Aligns to 11:89 / 377 (21.0%), covers 98.7% of PF01817, 79.3 bits
SGO_1386 chorismate mutase from Streptococcus gordonii str. Challis substr. CH1
Aligns to 7:84 / 88 (88.6%), covers 100.0% of PF01817, 79.0 bits
SPD_1151 chorismate mutase from Streptococcus pneumoniae D39
SP_1296 hypothetical protein from Streptococcus pneumoniae TIGR4
Aligns to 7:84 / 88 (88.6%), covers 98.7% of PF01817, 78.2 bits
ETAE_2836 bifunctional chorismate mutase/prephenate dehydrogenase from Edwardsiella tarda EIB202
Aligns to 9:87 / 373 (21.2%), covers 96.2% of PF01817, 77.7 bits
H16_A0792 prephenate dehydratase from Cupriavidus necator H16
H16_A0792 prephenate dehydratase, Chorismate mutase from Ralstonia eutropha H16
Aligns to 39:116 / 386 (20.2%), covers 100.0% of PF01817, 77.6 bits
CHMU_METJA / Q57696 Chorismate mutase; CM; Monofunctional chorismate mutase AroQ(f); EC 5.4.99.5 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Aligns to 9:87 / 99 (79.8%), covers 100.0% of PF01817, 76.4 bits
- function: Catalyzes the conversion of chorismate into prephenate via a Claisen rearrangement.
catalytic activity: chorismate = prephenate (RHEA:13897)
subunit: Homodimer.
SO1362 chorismate mutase/prephenate dehydrogenase from Shewanella oneidensis MR-1
Aligns to 13:91 / 379 (20.8%), covers 98.7% of PF01817, 76.3 bits
- Design and analysis of mismatch probes for long oligonucleotide microarrays
Deng, BMC genomics 2008 - “...Mm Na+/H+ exchanger MMP0926 44921025 Mm Chemotaxis protein cheB MMP1559 45047480 Mm Formatedehydrogenase alpha subunit SO1362 24371479 So Chorismate mutase/prephenate dehydrogenase (tyrA) SO1779 24371479 So Decaheme cytochrome c (omcA) SO2452 24371479 So Alcohol dehydrogenase, zinc-containing *DvH: Desulfovibrio vulgaris str. Hildenborough ; Mm: Methanococcus maripaludis ; So:...”
SMc03858 PUTATIVE CHORISMATE MUTASE PROTEIN from Sinorhizobium meliloti 1021
Aligns to 14:92 / 111 (71.2%), covers 97.5% of PF01817, 76.3 bits
B9DN54 Phospho-2-dehydro-3-deoxyheptonate aldolase / chorismate mutase from Staphylococcus carnosus (strain TM300)
Aligns to 8:85 / 362 (21.5%), covers 100.0% of PF01817, 75.9 bits
Echvi_0120 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase / chorismate mutase (EC 2.5.1.54; EC 5.4.99.5) from Echinicola vietnamensis KMM 6221, DSM 17526
Aligns to 276:354 / 367 (21.5%), covers 100.0% of PF01817, 75.6 bits
- mutant phenotype: Important for fitness in most defined media. The N-terminal DHAP region is only 33% identical to the Pyrococcus enzyme (Q8U0A9) but the active site and substrate-binding residues are conserved. The C-terminal chorismate mutase region is diverged but hits PF01817 and no other chorismate mutase was found in the genome.
Gmet_0862 Chorismate mutase, gamma, beta and epsilon from Geobacter metallireducens GS-15
Aligns to 21:98 / 368 (21.2%), covers 98.7% of PF01817, 75.0 bits
Ddes_0336 chorismate mutase from Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774
Aligns to 40:117 / 419 (18.6%), covers 98.7% of PF01817, 74.3 bits
- Coordinated response of the Desulfovibrio desulfuricans 27774 transcriptome to nitrate, nitrite and nitric oxide
Cadby, Scientific reports 2017 - “...membrane transport protein 1.79 0.00336 Ddes_0334 Prephenate dehydrogenase 1.85 0.00164 Ddes_0335 3-phosphoshikimate 1-carboxyvinyltransferase 2.29 4.16e-5 Ddes_0336 Chorismate mutase 2.47 3.53e-6 Ddes_0337 3-dehydroquinate synthase 1.87 0.00292 Ddes_0525 4Fe-4S ferredoxin family 1.94 0.00030 Ddes_0526 Pyridoxamine 5-phosphate oxidase-related FMN-binding 2.6 1.67e-6 Ddes_0527 Flavodoxin family protein 2.05 9.34e-5 Ddes_0528 CRP-family...”
- “...1.49 0.0132 J Ddes_0334 Prephenate dehydrogenase 1.52 0.0206 E Ddes_0335 3-phosphoshikimate 1-carboxyvinyltransferase 1.35 0.0377 E Ddes_0336 Chorismate mutase 1.56 0.00875 E Ddes_0337 3-dehydroquinate synthase 1.45 0.0119 E Ddes_0338 Fructose-bisphosphate aldolase 1.34 0.0334 G Ddes_0339 Pyridoxal phosphate-dependent D-cysteine desulfhydrase family 1.67 0.0392 E Ddes_0382 cooS Carbon monoxide...”
SCO2019 chorismate mutase from Streptomyces coelicolor A3(2)
Aligns to 23:101 / 116 (68.1%), covers 96.2% of PF01817, 74.1 bits
GSU2608 chorismate mutase/prephenate dehydratase from Geobacter sulfurreducens PCA
Aligns to 11:88 / 358 (21.8%), covers 98.7% of PF01817, 74.0 bits
D820_RS07095, SMU_531 chorismate mutase from Streptococcus mutans ATCC 25175
Aligns to 8:87 / 98 (81.6%), covers 98.7% of PF01817, 73.5 bits
- Transcriptomic Stress Response in Streptococcus mutans following Treatment with a Sublethal Concentration of Chlorhexidine Digluconate
Muehler, Microorganisms 2022 - “...D820_RS02990 4.00 10 6 Protein modification D820_RS05715, D820_RS02255, D820_RS08010 2.00 10 2 L-phenylalanine biosynthesis D820_RS03740, D820_RS07095 2.00 10 2 microorganisms-10-00561-t005_Table 5 Table 5 Validation of differentially expressed genes using qRT-PCR. Transcript levels of selected genes ( Table 1 ) were corrected to gyrB . Each value...”
- Genome-Wide Identification of Novel sRNAs in Streptococcus mutans
Krieger, Journal of bacteriology 2022 (secret) - [Transcriptomic Analysis of csn2 Gene Mutant Strains of Streptococcus mutans CRISPR-Cas9 System]
He, Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 2021 (secret) - Remodeling of the Streptococcus mutans proteome in response to LrgAB and external stresses
Ahn, Scientific reports 2017 - “...bottom). Notable protein abundance changes unique to aeration include upregulated accumulation of putative chorismate mutase (SMU_531), cell division protein FtsX, and putative mannose specific EIID component (SMU_1957), which were newly produced only in response to aeration, but not without aeration (Supplemental Table S3 ). It is...”
PGN_1053 putative phospho-2-dehydro-3-deoxyheptonate aldolase/chorismate mutase from Porphyromonas gingivalis ATCC 33277
PG0885 phospho-2-dehydro-3-deoxyheptonate aldolase/chorismate mutase from Porphyromonas gingivalis W83
Aligns to 275:353 / 366 (21.6%), covers 98.7% of PF01817, 73.3 bits
- Insights into Dynamic Polymicrobial Synergy Revealed by Time-Coursed RNA-Seq
Hendrickson, Frontiers in microbiology 2017 - “...PGN_0972 TPR motif PGN_0569 PGN_2050 PGN_0185 fimE PGN_1903 PGN_0172 PGN_1423 PGN_1673 PGN_0580 PGN_1413 PGN_0380 PGN_1145 PGN_1053 PGN_0180 fimA PGN_1507 PGN_0388 PGN_0972 TPR motif Figure 3 Differential expression of fimA locus genes ( PGN_0180 - PGN_0185 ) along with fimSR ( PGN_0903 and PGN_0904 ) in communities...”
- Regulon controlled by the GppX hybrid two component system in Porphyromonas gingivalis
Hirano, Molecular oral microbiology 2013 - “...0.01 PGN_1054 virulence modulating gene F 0.96 0.02 PGN_0997 putative deoxyuridine 5-triphosphate nucleotidohydrolase 0.88 0.04 PGN_1053 putative phospho-2-dehydro-3-deoxyheptonate aldolase/chorismate mutase 0.88 0.04 PGN_0315 precorrin-6x reductase/cobalamin biosynthetic protein CbiD 0.86 0.01 PGN_0316 precorrin-4 C11-methyltransferase 0.86 0.02 PGN_2023 putative phosphoribosylformylglycinamidine cyclo-ligase 0.81 0.03 PGN_1479 hypothetical protein 0.81 0.01...”
- Role of Acetyltransferase PG1842 in Gingipain Biogenesis in Porphyromonas gingivalis
Mishra, Journal of bacteriology 2018 - “...qRT-PCR. As shown in Figure 1, the vimE, vimF, PG0885 and PG0886 genes were 127 downregulated 3.6, 6.5, 5.6 and 2.8 fold respectively. 128 To further determine...”
- “...gingivalis FLL102, the expression levels of the vimE, vimF, PG0885 and PG0886 137 genes were determined by qRT-PCR. In contrast to FLL92, expression levels of...”
- Deletion of a 77-base-pair inverted repeat element alters the synthesis of surface polysaccharides in Porphyromonas gingivalis
Bainbridge, Journal of bacteriology 2015 - “...(PG1138 to PG1142) and the VimA locus (PG0880 to PG0885) (34); however, the nature of the alterations in LPS structure have not been elucidated. The 77bpIR...”
- “...synthesis, PG1138 to PG1142 (Fig. 6B) and PG0880 to PG0885 (data not shown), were examined in the 77bpIR strain, and expression was found to be unchanged or...”
- Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
Moon, BMC microbiology 2014 - “...126 R: TCCGGCTCATAGACTTCCAA PG1180 F: CAGTCTGCCACAGTTCACCA 124 R: CCCTACACGGACACTACCGA PG1983 F: GCTCTGTGGTGTGGGCTATC 146 R: GGATAACAGGCAAACCCGAT PG0885 F: CAGATCCAAATCGGGACTGA 156 R: GTAGAGCAAGCCATGCAAGC PG1181 F: GATGAATTCGGGCGGATAAT 184 R: CCTTGAAGTGCTCCAACGAC a Based on the genome annotation provided by TIGR ( http://cmr.jcvi.org/cgi-bin/CMR/GenomePage.cgi?org=gpg ). b Primers were designed using Primer3 program...”
SERP_RS06410 bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase from Staphylococcus epidermidis RP62A
SERP1297 chorismate mutase/phospho-2-dehydro-3-deoxyheptonate aldolase from Staphylococcus epidermidis RP62A
Aligns to 9:86 / 363 (21.5%), covers 100.0% of PF01817, 73.0 bits
M271_36305 chorismate mutase from Streptomyces rapamycinicus NRRL 5491
Aligns to 34:112 / 129 (61.2%), covers 96.2% of PF01817, 72.9 bits
PFLU_1772 chorismate mutase from Pseudomonas fluorescens SBW25
Aligns to 48:127 / 139 (57.6%), covers 98.7% of PF01817, 72.6 bits
MLD56_05715 bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase from Paenibacillus peoriae
Aligns to 12:89 / 360 (21.7%), covers 100.0% of PF01817, 72.4 bits
BA2958 chorismate mutase/phospho-2-dehydro-3-deoxyheptonate aldolase from Bacillus anthracis str. Ames
GBAA2958 chorismate mutase/phospho-2-dehydro-3-deoxyheptonate aldolase from Bacillus anthracis str. 'Ames Ancestor'
GBAA_2958 bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase from Bacillus anthracis str. 'Ames Ancestor'
Aligns to 10:87 / 358 (21.8%), covers 100.0% of PF01817, 72.3 bits
- Identification of the immunogenic spore and vegetative proteins of Bacillus anthracis vaccine strain A16R
Liu, PloS one 2013 - “...BALH_0915 31558 6.01 [41] r006 - BA5588 - 3-hydroxybutyryl-CoA dehydrogenase 31533 5.45 [41] r024 - BA2958 - 3-deoxy-7-phosphoheptulonate synthase 40115 5.65 [41] r154 - BA1998 nadE NH(3)-dependent NAD(+) synthetase 30083 4.94 [41] r014 - BA5061 - hypothetical protein 25273 5.8 [41] - r205\r206 BA5017 metK S-adenosylmethionine...”
- Strand-specific RNA-seq reveals ordered patterns of sense and antisense transcription in Bacillus anthracis
Passalacqua, PloS one 2012 - “...Ratio nanoString spore germination protein gerD GBAA0148 0.22 27.91 71 943 0.01 0.08 3-deoxy-7-phosphoheptulonate synthase GBAA2958 600.93 0.04 90,013 1 13,654.02 90,012.77 sodA1 : superoxide dismutase, Mn GBAA4499 2,665.50 0.00 257,357 1 * 2,665.50 257,357.45 RNA polymerase sigma factor sigA GBAA4515 201.86 45.67 88,517 29,661 4.42...”
- Effect of over expressing protective antigen on global gene transcription in Bacillus anthracis BH500
Sharma, Scientific reports 2018 - “...peptides, high regulation of amino acid biosynthesis genes such as ilvN (3.02), tyrA (1.83), and GBAA_2958 (1.90) was observed in the lag phase of the PA-expressing cells, probably to fulfil the requirement for precursor amino acids needed for PA expression. As the culture advanced into the...”
FOA15_RS11190 bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase from Paenibacillus kribbensis
Aligns to 12:89 / 360 (21.7%), covers 100.0% of PF01817, 72.1 bits
A8AAX2 prephenate dehydrogenase (NADP+) (EC 1.3.1.13) from Ignicoccus hospitalis (see 2 papers)
Aligns to 13:91 / 348 (22.7%), covers 98.7% of PF01817, 72.0 bits
lp_2038 transport protein from Lactobacillus plantarum WCFS1
lp_2038 MFS transporter from Lactiplantibacillus plantarum WCFS1
Aligns to 393:470 / 484 (16.1%), covers 98.7% of PF01817, 71.9 bits
- Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study
Petrov, Genes 2021 - “...1-carboxyvinyltransferase 2.27 +2.52 lp_2113 Iron-sulfur cluster biosynthesis protein 2.36 +2.17 lp_1992 Hypothetical protein 2.02 +2.09 lp_2038 MFS transporter 2.17 +2.42 lp_1955 ABC transporter permease subunit 3.19 +5.81 * The difference in gene expression was estimated as the fold change (FC) of mRNA encoding the respective protein...”
VVMO6_04207 isochorismate lyase from Vibrio vulnificus MO6-24/O
VV20839, VV2_0839 Chorismate mutase from Vibrio vulnificus CMCP6
Aligns to 17:94 / 109 (71.6%), covers 98.7% of PF01817, 71.9 bits
- MARTX Toxin-Stimulated Interplay between Human Cells and Vibrio vulnificus
Kim, mSphere 2020 - “...mice ( 59 , 60 ), such as the genes encoding isochorismatase (VVMO6_04206), isochorismate-pyruvate lyase (VVMO6_04207), and 2, 3-dihydroxybenzoate-2, 3-dehydrogenase (VVMO6_04201), were also more highly expressed in WT V. vulnificus than in the rtxA1 strain (5.5-, 5.9-, and 6.7-fold, respectively) ( Fig.5 ; see also TableS3...”
- The immediate global responses of Aliivibrio salmonicida to iron limitations
Thode, BMC microbiology 2015 - “...of the eight unique transcripts encode proteins that are involved in vulnibactin biosynthesis and transport (VV20839, VV20840, VV20841 and VV20844), and the remaining four have functions in iron transport, i.e. TonB systems (VV21618, VV20359 and VV20360) and an ABC-type Fe 3+ transport protein (VV11662). In A....”
- Molecular characterization of vulnibactin biosynthesis in Vibrio vulnificus indicates the existence of an alternative siderophore
Tan, Frontiers in microbiology 2014 - “...on genes around SA and 2, 3-DHBA biosynthetic steps. Deletion mutant of isochorismate pyruvate lyase (VV2_0839) or 2, 3-dihydroxybenzoate-2, 3-dehydrogenase (VV2_0834) showed retarded growth under iron-limited conditions though the latter showed more significant growth defect than the former, suggesting a dominant role of 2, 3-DHBA in...”
- “...four genes in the mentioned gene cluster (Figure 1A ) i.e., VV2_0834 (2, 3-dihydroxybenzoate-2, 3-dehydrogenase), VV2_0839 (isochorismate pyruvate lyase), and two genes encoding putative AMP ligases (VV2_0836 and VV2_0840) for activation of 2, 3-DHBA or SA. By gene deletion and respective in-trans complementation studies we establish...”
- Global gene expression as a function of the iron status of the bacterial cell: influence of differentially expressed genes in the virulence of the human pathogen Vibrio vulnificus
Alice, Infection and immunity 2008 - “...biosynthesis and transport VV20834 VV20835 VV20837 VV20838 VV20839 VV20840 VV20841 VV20842 Annotated function (gene name) VOL. 76, 2008 IRON REGULATION IN...”
P74964 chorismate mutase (Fragment) from Vibrio vulnificus
Aligns to 25:102 / 117 (66.7%), covers 98.7% of PF01817, 71.7 bits
SXYL_01128 bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase from Staphylococcus xylosus
Aligns to 9:86 / 363 (21.5%), covers 100.0% of PF01817, 71.7 bits
- Adaptation of Staphylococcus xylosus to Nutrients and Osmotic Stress in a Salted Meat Model
Vermassen, Frontiers in microbiology 2016 - “...aroD 3-dehydroquinate dehydratase 0.2 0.3 0.3 SXYL_01497 trpA Tryptophan synthase alpha chain 0.4 0.4 0.5 SXYL_01128 DAHP synthetase-chorismate mutase 0.1 0.0 0.1 Histidine SXYL_00460-68 hisZGDCBHAFI Histidine biosynthesis 0.1 * 0.1 * 0.1 * Cysteine, methionine SXYL_02644 metE 5-methyltetrahydropteroyltriglutamatehomocysteine methyltransferase 0.1 0.1 0.2 SXYL_00372 D-cysteine desulfhydrase 0.3...”
- “...cluster ilv-leu and the gene ilvD2 ), aromatic amino acids ( aroABC, aroD, trpA , SXYL_01128), histidine ( hisZGDCBHAFI ) and cysteine-methionine ( metE , SXYL_00372) (Table 1 , Figure 1 ). In S. aureus , CodY contributes to the regulation of more than 200 genes...”
- Transcriptomic analysis of Staphylococcus xylosus in the presence of nitrate and nitrite in meat reveals its response to nitrosative stress
Vermassen, Frontiers in microbiology 2014 - “...trpG Anthranilate synthase component II 0.5 0.4 SXYL_01503 trpE Anthranilate synthase component I 0.5 0.4 SXYL_01128 DAHP synthetase-chorismate mutase 0.5 SXYL_01513 tyrA Prephenate dehydrogenase 3.1 SXYL_00922 pheA Prephenate dehydratase 0.6 SXYL_02022 aroD 3-dehydroquinate dehydratase 1.7 SXYL_01261 aroE Shikimate dehydrogenase 0.6 SXYL_01316 aroK Shikimate kinase 0.5 Urea,...”
- “...most Gram-positive bacteria (Gutierrez-Preciado et al., 2005 ). Six of these genes and the gene SXYL_01128 encoding a DAHP synthase-chorismate mutase were down-regulated in our conditions (Table 1 ). Nitrosative stress can also modulate the expression of genes involved in sulfur-containing amino acids synthesis (Figure 4...”
PP2170 chorismate mutase family protein from Pseudomonas putida KT2440
Aligns to 142:219 / 232 (33.6%), covers 98.7% of PF01817, 71.4 bits
- Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: genetic and physiological basis for improved production
Wierckx, Journal of bacteriology 2008 - “...(aroF-1), PP0074 (aroE-1), PP3002 (aroE-2), PP1769 (pheA), PP2170, PP1770, PP4490 (phhA), PP1972 (tyrB-1), PP3590 (tyrB-2), and PP4621 (hmgA). Expression...”
SAPIG1790 bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase from Staphylococcus aureus subsp. aureus ST398
Aligns to 9:86 / 363 (21.5%), covers 100.0% of PF01817, 71.3 bits
SAUSA300_1683 chorismate mutase/phospho-2-dehydro-3-deoxyheptonate aldolase from Staphylococcus aureus subsp. aureus USA300_FPR3757
SA1558 3-deoxy-7-phosphoheptulonate synthase from Staphylococcus aureus subsp. aureus N315
SAV1737 3-deoxy-7-phosphoheptulonate synthase from Staphylococcus aureus subsp. aureus Mu50
SAOUHSC_01852 hypothetical protein from Staphylococcus aureus subsp. aureus NCTC 8325
MW1680 ORFID:MW1680~chorismate mutase homolog from Staphylococcus aureus subsp. aureus MW2
SACOL1787 chorismate mutase/phospho-2-dehydro-3-deoxyheptonate aldolase from Staphylococcus aureus subsp. aureus COL
Aligns to 9:86 / 363 (21.5%), covers 100.0% of PF01817, 71.3 bits
- Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Genetic Factors Involved in Human Endothelial Cells Damage, an Important Phenotype Correlated with Persistent Endovascular Infection
Xiao, Antibiotics (Basel, Switzerland) 2022 - “...hisF imidazole glycerol phosphate synthase subunit HisF 27.62 4.01 SAUSA300_0795 hypothetical hypothetical protein 27.38 6.00 SAUSA300_1683 hypothetical bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase 27.26 6.86 SAUSA300_2618 hypothetical hypothetical protein 27.23 7.65 SAUSA300_1398 hypothetical phiSLT ORF123-like protein 27.16 11.43 SAUSA300_0059 hypothetical conserved hypothetical protein 27.07 7.67 SAUSA300_1764 epiD lantibiotic...”
- Growth and Stress Tolerance Comprise Independent Metabolic Strategies Critical for Staphylococcus aureus Infection
Kim, mBio 2021 - “...3210 908 + + + + SAUSA300_2064 atpB 8239 10075 981 + + + + SAUSA300_1683 aroA2 228 ** 2414 * 958 + + + + SAUSA300_1090 3911 * 5217 951 + + + + SAUSA300_1183 4325 * 10229 1010 + + + 1,586 422 ***...”
- “...0.480.14 SAUSA300_1465 bfmBAB 5 0.640.01 **** 3.35 125.6741.29 ** 221.6175.28 **** 53.1820.27 ** 0.770.15 ** SAUSA300_1683 aroA2 5.62 1.020.02 3.23 95.515.95 97.6643.35 38.219.71 0.70.26 SAUSA300_2065 mnaA 6.29 1.090.03 ** 3.21 127.7132.97 * 129.1329.51 35.678.12 0.770.23 * SAUSA300_2439 galU 5.82 1.030.07 3.2 93.720.45 76.7521.64 24.751.53 * 1.370.40...”
- Novel Pathways for Ameliorating the Fitness Cost of Gentamicin Resistant Small Colony Variants
Vestergaard, Frontiers in microbiology 2016 - “...(HemH Glu263* and HemB Pro240Leu) biosynthesis pathways. The fifth strain contained a deletion in the SAUSA300_1683 gene (SAUSA300_1683 Leu274fs), encoding for the bi-functional enzyme 3-deoxy-7-phosphoheptulonate synthase [EC: 2.5.1.54]/chorismate mutase [EC: 5.4.99.5]. This enzyme is required for the biosynthesis of chorismate, the first precursor molecule of the...”
- “...(1632 g/ml), compared to the MIC of the WT strain (2 g/ml). The menD and SAUSA300_1683 displayed menadione auxotrophy, while the hemH and hemB displayed hemin auxotrophy. Growth complementation with menadione could not be achieved for menA ( Lannergrd et al., 2011 ) ( Table 2...”
- Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
Anderson, FEMS immunology and medical microbiology 2010 - “...ND SA1501 hypothetical protein sa_c9728s8474_a_at 3.6 2.5 2.5 SA1512 hypothetical protein sa_c2489s2070_at 3.3 15 2.5 SA1558 hypothetical protein sa_c2533s2114_at 4.6 2.5 2.5 SA1570 hypothetical protein sa_c10583s11040_a_at 2.3 2.5 ND SA1585 hypothetical protein sa_c2611s2187_a_at 2.3 2.5 2.5 SA1605 hypothetical protein sa_c10360s10752_s_at 2.3 2.5 2.5 SA1606 rhomboid family...”
- Transcriptomic and Metabolomic Analysis of a Fusidic Acid-Selected fusA Mutant of Staphylococcus aureus
Gupta, Antibiotics (Basel, Switzerland) 2022 - “...asd , cysE , glyA , hisF , SAS0418, and SAS2563) than upregulated (SACOL2044 and SAV1737) in SH10001st-2 ( Tables S1 and S2 ). With regard to carbohydrate transporters, 3 (SACOL2146, SACOL2552, and treP ) were downregulated and another 2 (SAV0192 and ptaA ) were upregulated...”
- Inhibition of Staphylococcus aureus biofilm formation by gurmarin, a plant-derived cyclic peptide
Chang, Frontiers in cellular and infection microbiology 2022 - “...0.03 SACOL1518 -2.66 Cytidylate kinase cmk 0.01 SACOL2054 -2.59 RNA polymerase sigma-37 factor rpoF 0.02 SAV1737 -2.59 3-deoxy-7-phosphoheptulonate synthase 0.02 SACOL2353 -2.58 Transcriptional regulator tcaR 0.05 SAV2651 -2.57 Hypothetical protein 0.04 MW2255 -2.46 Hypothetical protein 0.02 SACOL0962 -2.45 Glycerophosphoryl diester phosphodiesterase GlpQ, putative glpQ 0.04 SACOL1410...”
- Interplay of CodY and CcpA in Regulating Central Metabolism and Biofilm Formation in Staphylococcus aureus
Bulock, Journal of bacteriology 2022 (secret) - CidR and CcpA Synergistically Regulate Staphylococcus aureus cidABC Expression
Sadykov, Journal of bacteriology 2019 (secret) - The SAV1322 gene from Staphylococcus aureus: genomic and proteomic approaches to identification and characterization of gene function
Kim, BMC microbiology 2016 - “...A060 gi|15924160 Carbamate kinase 990 arcC1 SAV1170 Up A280 gi|15924727 Bifunctional 3-deoxy-7-phophoheptulonate sunthase/chorismate mutase 434 SAV1737 Up Cell division, cell wall, cell envelope biogenesis A017 gi|15925144 Glucosamine--fructiose-6-phosphate aminotransferase 167 glmS SAV2154 Down A032 gi|15925072 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase 792 murF SAV2082 Down A033 gi|15925114 UDP-N-acetylglucosamine-1-carboxylvinyl transferase 1062 murZ...”
- The ORFeome of Staphylococcus aureus v 1.1
Brandner, BMC genomics 2008 - “...SAV1330 Homoserine kinase 304 AA [ 38 ] SAV0217 Similar to NADH-dependent dehydrogenase 359 AA SAV1737 Chorismate mutase homolog 363 AA SAV1235 Signal recognition particle 416 AA SAV1627 Similar to ATPase, AAA family 424 AA SAV1454 Asparaginyl-tRNA synthetase 430 AA [ 39 ] SAV2121 Transcription termination...”
- A new platform for ultra-high density Staphylococcus aureus transposon libraries
Santiago, BMC genomics 2015 - “...biosynthetic pathways (SAOUHSC_01481, aroB: SAOUHSC_01482, aroF: SAOUHSC_01483, menF: SAOUHSC_00982, menD: SAOUHSC_00983, aroE: SAOUHSC_01699, and aroA: SAOUHSC_01852) (Figure 7 A). In addition to producing phenylalanine and tyrosine, the aromatic amino acid pathway provides precursors (chorismate) for menaquinone biosynthesis (Figure 7 B) [ 82 ]. Menaquinones are isoprenylated...”
- Global analysis of community-associated methicillin-resistant Staphylococcus aureus exoproteins reveals molecules produced in vitro and during infection
Burlak, Cellular microbiology 2007 - “...12 23 426 Cysteine synthase (CysK) C2 82750220 32969 5.4 8 52 273 3-Deoxy-7-phosphoheptulonate synthase (MW1680) B1,D1 49483977 40609 5.8 7 24 229 Glucosamine-fructose 6-phosphate aminotransferase (GlmS) D1 14247927 65795 4.9 5 11 110 Glutamine synthetase (GlnA) B1 1134886 50808 5.1 11 34 400 Imidazolonepropionase (HutI)...”
- Global analysis of the Staphylococcus aureus response to mupirocin
Reiss, Antimicrobial agents and chemotherapy 2012 - “...PepF SACOL1365 SACOL1562 SACOL1562-1 SACOL1759 SACOL1787 SACOL1759-1 SACOL1759-2 SACOL1787-2 SACOL1891 SACOL1975 SACOL1891-1 SACOL1975 SACOL2106 SACOL2106...”
- Staphylococcus aureus CcpA affects virulence determinant production and antibiotic resistance
Seidl, Antimicrobial agents and chemotherapy 2006 - “...utilization protein A and C, and saCOL1787, thought to encode a chorismate mutase-phospho-2-dehydro-3-deoxyheptonate aldolase, yielded identical patterns for...”
VC0705 chorismate mutase/prephenate dehydratase from Vibrio cholerae O1 biovar eltor str. N16961
Aligns to 12:91 / 391 (20.5%), covers 100.0% of PF01817, 70.9 bits
- Systematic Identification of Cyclic-di-GMP Binding Proteins in Vibrio cholerae Reveals a Novel Class of Cyclic-di-GMP-Binding ATPases Associated with Type II Secretion Systems
Roelofs, PLoS pathogens 2015 - “...MshE sequence and structure. (A) Sequence alignment of the N-terminal fragment of Vibrio cholerae MshE (VC0705) with PA14_29490 from Pseudomonas aeruginosa , GspE proteins from Xanthomonas campestris (UniProt: GPSE_XANCP, PDB: 2D27) and V . cholerae (GPSE_VIBCH, PDB: 2BH1). (B) Structure of the N-terminal domain of GSPE_XANCP...”
- Mining regulatory 5'UTRs from cDNA deep sequencing datasets
Livny, Nucleic acids research 2010 - “...instead, it likely corresponds to the V. cholerae LeuL leader peptide. Candidate No. 4 : vc0705 is a homologue of the gene encoding the E. coli PheA which is subject to co-transcriptional regulation through a leader peptide ( 33 ). Indeed, as shown in Figure 2...”
- “...50S ribosomal protein L13 L13 leader(64) RLE0227(17) 3 VC0647 Polynucleotide phosphorylase/polyadenylase S15(1) RLE0154I(8) 4 * VC0705 Chorismate mutase/prephenate dehydratase T-box(11), T-box(1) 5 VC0875 Prolyl-tRNA synthetase T-box( 15 ) RLE018(5) 6* VC0894 Thiamine biosynthesis protein ThiI SAM-IV(1) RLE0079(7) 7 VC1091 Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein T-box(4),...”
- Identification and characterization of a Vibrio cholerae gene, mbaA, involved in maintenance of biofilm architecture
Bomchil, Journal of bacteriology 2003 - “...c, and d open reading frames correspond to loci VC0705, VC0704, VC0703, VC0702, and VC0701, respectively, based on the DNA sequences obtained from The Institute...”
Ssal_00456 chorismate mutase from Streptococcus salivarius 57.I
Aligns to 8:87 / 92 (87.0%), covers 100.0% of PF01817, 70.8 bits
Q49YG8 DAHP synthetase-chorismate mutase from Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41)
Aligns to 9:86 / 363 (21.5%), covers 100.0% of PF01817, 70.7 bits
- In Silico Subtractive Proteomics Approach for Identification of Potential Drug Targets in Staphylococcus saprophyticus
Shahid, International journal of environmental research and public health 2020 - “...Putative preprotein translocase subunit (Q49Y76) ssp03060-Protein Export ssp02024-Quorum sensing ssp03070-Bacterial secretion system DAHP synthetase-chorismate mutase (Q49YG8) ssp01230-Biosynthesis of amino acids ssp01100-Metabolic pathways ssp00400-Phenylalanine, tyrosine, and tryptophan biosynthesis ssp01110-Biosynthesis of secondary metabolites ssp01130-Biosynthesis of antibiotics Putative heptaprenyl diphosphate synthase component (Q49XS4) ssp00900-Terpenoid backbone biosynthesis ssp01110-Biosynthesis of secondary...”
XHV734_3366 prephenate dehydratase from Xanthomonas hortorum pv. vitians
Aligns to 48:127 / 399 (20.1%), covers 100.0% of PF01817, 70.6 bits
1ecmB / P0A9J8 Atomic structure of the buried catalytic pocket of escherichia coli chorismate mutase
Aligns to 6:84 / 95 (83.2%), covers 100.0% of PF01817, 70.5 bits
- Ligand: 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid (1ecmB)
CMPDT_STUST / P27603 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Stutzerimonas stutzeri (Pseudomonas stutzeri) (see paper)
Aligns to 11:93 / 365 (22.7%), covers 100.0% of PF01817, 70.5 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate and the decarboxylation/dehydration of prephenate to phenylpyruvate.
catalytic activity: chorismate = prephenate (RHEA:13897)
catalytic activity: H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O (RHEA:21648)
O87954 Bifunctional chorismate mutase/prephenate dehydratase from Xanthomonas campestris
Aligns to 49:128 / 400 (20.0%), covers 100.0% of PF01817, 70.1 bits
XC_2644 P-protein from Xanthomonas campestris pv. campestris str. 8004
Aligns to 55:134 / 406 (19.7%), covers 100.0% of PF01817, 70.0 bits
PA3166 chorismate mutase from Pseudomonas aeruginosa PAO1
Aligns to 11:93 / 365 (22.7%), covers 100.0% of PF01817, 70.0 bits
- Massively parallel mutant selection identifies genetic determinants of Pseudomonas aeruginosa colonization of Drosophila melanogaster
Miles, mSystems 2024 - “...Nucleotide metabolism [F] PA0430 metF Amino acids [E] + PA3107 metZ Amino acids [E] + PA3166 pheA Amino acids [E] PA5277 lysA Amino acids [E] PA0025 aroE Amino acids [E] PA5038 aroB Amino acids [E] PA5039 aroK Amino acids [E] PA4846 aroQ1 Amino acids [E] PA3165...”
- Bacterial fitness in chronic wounds appears to be mediated by the capacity for high-density growth, not virulence or biofilm functions
Morgan, PLoS pathogens 2019 - “...25.5 0.011 nt PA4756 carB 1709 18.1 0.011 nt PA2141 - 263 2.9 0.011 nt PA3166 pheA 675 7.4 0.011 nt PA5004 - 1126 12.4 0.011 nt * all reads for each sample were normalized to 10,000,000 and divided by the gene length (Kb) and averaged...”
- A systems biology approach to drug targets in Pseudomonas aeruginosa biofilm
Sigurdsson, PloS one 2012 - “...AroC (PA1681), AroK (PA5039) AroB (PA5038), AroA (PA3164), AroE (PA0025) TrpA (PA0035), TrpB (PA0036), PheA (PA3166) Biosynthesis of cofactors, prosthetic groups and carriers, cell wall/ Lipopolysaccharide/ capsule UppS (PA3652) Biotin biosynthesis AccC (PA4848) Branched chain amino acid biosynthesis IlvC (PA4694), IlvD (PA0353) Cell envelope biosynthesis GlmU...”
- Growth phenotypes of Pseudomonas aeruginosa lasR mutants adapted to the airways of cystic fibrosis patients
D'Argenio, Molecular microbiology 2007 - “...product CF5296/CF416a CF416lasR::Gm/CF416a PA0510b PA1913 PA2112b PA2625 PA3166 PA3366 PA3392 PA3976 PA4329 PA4442b PA4486b PA4726 PA5435 NirE 14.29 100 4.55...”
CMPDT_BUCAI / P57472 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) (see paper)
P57472 prephenate dehydratase (EC 4.2.1.51) from Buchnera aphidicola (see paper)
Aligns to 11:89 / 385 (20.5%), covers 100.0% of PF01817, 69.9 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate and the decarboxylation/dehydration of prephenate to phenylpyruvate.
catalytic activity: chorismate = prephenate (RHEA:13897)
catalytic activity: H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O (RHEA:21648)
SSA_1268 Chorismate mutase/prephenate dehydratase, putative from Streptococcus sanguinis SK36
Aligns to 7:84 / 87 (89.7%), covers 100.0% of PF01817, 69.6 bits
PP1769 chorismate mutase/prephenate dehydratase from Pseudomonas putida KT2440
Aligns to 13:95 / 367 (22.6%), covers 100.0% of PF01817, 69.4 bits
- Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440
Molina-Henares, Microbial biotechnology 2009 - “...growth was restored only by phenylalanine, miniTn 5 was inserted at different sites within ORF PP1769 ( pheA ). The insertion in the pheA mutant was located in the 6th, 37th, 42nd and 120th codon (Aux6 to 9; Table1 ). Growth of the pheA mutants was...”
- “...as 0.3 units at 660nm. Physical analysis of the ORF PP1769/PP1770 region revealed that ORF PP1769 overlapped with the preceding gene annotated as serC by six nucleotides, and was separated from ORF PP1770 by 61 nucleotides ( Fig.4 ). As the two ORFs identified as involved...”
- Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: genetic and physiological basis for improved production
Wierckx, Journal of bacteriology 2008 - “...PP1866, PP2324 (aroF-1), PP0074 (aroE-1), PP3002 (aroE-2), PP1769 (pheA), PP2170, PP1770, PP4490 (phhA), PP1972 (tyrB-1), PP3590 (tyrB-2), and PP4621 (hmgA)....”
ZMO0563 chorismate mutase from Zymomonas mobilis subsp. mobilis ZM4
Aligns to 10:88 / 100 (79.0%), covers 96.2% of PF01817, 69.1 bits
- Model-driven analysis of mutant fitness experiments improves genome-scale metabolic models of Zymomonas mobilis ZM4
Ong, PLoS computational biology 2020 - “...Annotation ZMO0201 Glutamine amidotransferase of 4-amino-4-deoxychorismate synthase (isozyme) MEGS pabA Glutamine amidotransferase of anthranilate synthase ZMO0563 Chorismate-pyruvate lyase MEGS ubiC Chorismate mutase ZMO1008 Erythronate-4-phosphate dehydrogenase MEGS pdxB FAD linked oxidase domain protein ZMO1518 Histidinol phosphatase Bar-Seq Correlation N/A Inositol-monophosphatase ZMO1916 Pimeloyl-ACP methyl ester esterase MEGS bioH...”
- “...ZMO1916 (annotated as a hypothetical protein) likely encodes a pimeloyl-ACP methyl ester esterase. ZMO0562 and ZMO0563 were found together on the plasmid complementing the growth of the ubiC E . coli mutant. These two genes were cloned separately into the E . coli ubiC strain and...”
ACIAD2223 bifuctional protein [Includes: chorismate mutase P; prephenate dehydratase ] from Acinetobacter sp. ADP1
Aligns to 18:97 / 369 (21.7%), covers 98.7% of PF01817, 69.1 bits
MMP0578 Chorismate mutase from Methanococcus maripaludis S2
Aligns to 13:91 / 96 (82.3%), covers 100.0% of PF01817, 69.0 bits
PGA1_c00590 chorismate mutase from Phaeobacter inhibens DSM 17395
Aligns to 20:98 / 102 (77.5%), covers 97.5% of PF01817, 69.0 bits
- The marine bacterium Phaeobacter inhibens secures external ammonium by rapid buildup of intracellular nitrogen stocks
Trautwein, FEMS microbiology ecology 2018 - “...involved in phenylacetate degradation; PaaZ2, oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberoyl-CoA semialdehyde dehydrogenase (NADP + ); PatB, cystathionine beta-lyase; PGA1_c00590, chorismate mutase; PGA1_c07640, O -acetylserine sulfhydrylase; PGA1_c20760, putative nitrite/sulfite reductase; PGA1_c24800, putative bifunctional SAT/APS kinase; PGA1_c30200, prephenate dehydratase; SerA, 3-phosphoglycerate dehydrogenase; SerB, phosphoserine phosphatase; SerC, phosphoserine aminotransferase; TdaA, transcriptional regulator,...”
plu3564 No description from Photorhabdus luminescens subsp. laumondii TTO1
Aligns to 22:101 / 119 (67.2%), covers 98.7% of PF01817, 68.8 bits
- Photorhabdus luminescens genes induced upon insect infection
Münch, BMC genomics 2008 - “...putative methylase and protoporphyrinogen oxidase plu3565 similar to class II aminotransferase and 5-aminolevulinic acid synthase plu3564 weakly similar to PapB protein and to chorismate mutase/prephenate dehydrogenase plu3563 similar to p-aminobenzoic acid synthase plu3562 similar to dehydrogenase PapC of Streptomyces pristinaespiralis plu3561 probable transport protein Unknown function...”
str1594 hypothetical protein from Streptococcus thermophilus CNRZ1066
Aligns to 11:90 / 96 (83.3%), covers 100.0% of PF01817, 68.6 bits
Ddes_1346 chorismate mutase related enzyme from Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774
Aligns to 14:91 / 107 (72.9%), covers 98.7% of PF01817, 68.5 bits
5gmuB / P39912 Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
Aligns to 9:86 / 87 (89.7%), covers 100.0% of PF01817, 68.2 bits
- Ligand: (1r,3r,4s,5r)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid (5gmuB)
6al9B / O25064 Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
Aligns to 9:84 / 91 (83.5%), covers 100.0% of PF01817, 67.9 bits
- Ligand: pyruvic acid (6al9B)
O25064 Chorismate mutase domain-containing protein from Helicobacter pylori (strain ATCC 700392 / 26695)
HP0291 hypothetical protein from Helicobacter pylori 26695
Aligns to 13:88 / 96 (79.2%), covers 100.0% of PF01817, 67.7 bits
- Alteration of the Helicobacter pylori membrane proteome in response to changes in environmental salt concentration
Voss, Proteomics. Clinical applications 2015 - “...metabolic process 2.27 glucosamine fructose-6-phosphate aminotransferase (isomerizing) (GlmS) HP0721 O25423 NA 2.08 hypothetical protein HP0291 O25064 chorismate metabolic process 2.02 hypothetical protein HP0610 * # O25331 NA 2.01 toxin-like outer membrane protein (FaaA) Proteins more abundant in high salt compared to low salt Accession Uniprot KEGG...”
- The Role of a Dipeptide Transporter in the Virulence of Human Pathogen, Helicobacter pylori
Xu, Frontiers in microbiology 2021 - “...HP0213 gidA 2.3523 HP0229 omp6 1.3943 HP0243 napA 1.9605 HP0289 ImaA 1.3117 HP0290 lysA 1.5474 HP0291 Predicted gene 1.2657 HP0292 Predicted gene 1.0587 HP0294 amiE 2.2148 HP0296 rplU 1.4814 HP0297 rpmA 1.4635 HP0298 dppA 4.9784 HP0299 dppB 1.4717 HP0300 dppC 2.088 HP0301 dppD 2.4619 HP0302 dppF...”
- High-Salt Conditions Alter Transcription of Helicobacter pylori Genes Encoding Outer Membrane Proteins
Loh, Infection and immunity 2018 - “...HPB8_1525 HPB8_1526 HPB8_1551 HPB8_1702 HP0325 HP0318 HP0296 HP0291 HP0256 HP0227 HP0220 HP0189 HP0115 HP0073 HP0072 HP0057 HP1354 HP1355 HP1382 HP1400 pspA,...”
- “...HP0630 HP0642 HP0696 HP0731 HP0751 HP0802 HP0809 HP0318 HP0291 HP0229 HP0135 HP0115 HP0072 HP0025 HP1399 HP1400 jhp0828 Gene name or function pspA rplW...”
- Alteration of the Helicobacter pylori membrane proteome in response to changes in environmental salt concentration
Voss, Proteomics. Clinical applications 2015 - “...in this analysis (LysA and a hypothetical protein) are encoded by adjacent genes (HP0290 and HP0291) that are predicted to be members of an operon. Thirteen proteins were more abundant in bacteria grown under high salt conditions ( Table 1 ). These included CagA, flagellar hook...”
- “...glutamine metabolic process 2.27 glucosamine fructose-6-phosphate aminotransferase (isomerizing) (GlmS) HP0721 O25423 NA 2.08 hypothetical protein HP0291 O25064 chorismate metabolic process 2.02 hypothetical protein HP0610 * # O25331 NA 2.01 toxin-like outer membrane protein (FaaA) Proteins more abundant in high salt compared to low salt Accession Uniprot...”
- Expanded metabolic reconstruction of Helicobacter pylori (iIT341 GSM/GPR): an in silico genome-scale characterization of single- and double-deletion mutants
Thiele, Journal of bacteriology 2005 - “...TYRt2r ................................................................................................................................HP0291 (CHORM), HP1380...”
- Global analysis of Helicobacter pylori gene expression in human gastric mucosa
Graham, Gastroenterology 2002 - “...JHP0960 HP0245 HP0602 HP1002 HP1349 c JHP0756 JHP1306 HP0291 HP0645 c HP1015 c HP1358 JHP0814 JHP1462 Array features for 44 ORFs showing differential...”
5j6fA / L8A208 Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
Aligns to 8:85 / 352 (22.2%), covers 100.0% of PF01817, 67.6 bits
- Ligands: manganese (ii) ion; prephenic acid (5j6fA)
DVU0462 chorismate mutase/prephenate dehydratase from Desulfovibrio vulgaris Hildenborough
Aligns to 22:99 / 391 (19.9%), covers 98.7% of PF01817, 67.3 bits
CHMU_SALTM / Q93LJ4 Monofunctional chorismate mutase; EC 5.4.99.5 from Salmonella typhimurium (see paper)
SL1344_1204 chorismate mutase from Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
STM1269 putative chorismate mutase from Salmonella typhimurium LT2
Aligns to 29:99 / 181 (39.2%), covers 87.3% of PF01817, 67.2 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate.
catalytic activity: chorismate = prephenate (RHEA:13897) - The architecture and ppGpp-dependent expression of the primary transcriptome of Salmonella Typhimurium during invasion gene expression
Ramachandran, BMC genomics 2012 - “...untreated control. First strand cDNA synthesis was performed using either random hexamers oligonucleotide primers (for SL1344_1204 and SL1344_1122) or gene-specific primers followed by PCR amplification with nested gene-specific primers and 5' Adaptor-specific DNA primer B6. Resulting PCR products were cloned into the pGEM -T Easy vector...”
- Contribution of the Cpx envelope stress system to metabolism and virulence regulation in Salmonella enterica serovar Typhimurium
Subramaniam, PloS one 2019 - “...( aldB , bioA , eco , glpT , pfkB , pgtE , ssrB , STM1269 , STM2613 , STM3681 , STM4562 , tatA , ybiJ , yciG ) ( Fig 2C ). These potential novel CpxR target genes were clustered into genes with uncharacterized products...”
- Comparison of Salmonella enterica serovar Typhimurium LT2 and non-LT2 salmonella genomic sequences, and genotyping of salmonellae by using PCR
Kim, Applied and environmental microbiology 2006 - “...310 63 STM4571 154 65 STM2453 270 65 STM2624 284 65 STM1269 425 65 STM1277 289 65 STM0538 288 65 STM3532 233 65 7 STM4509 312 65 8 STM0305 155 65 STM1408...”
- Salmonella enterica highly expressed genes are disease specific
Rollenhagen, Infection and immunity 2006 - “...STM1275 STM0701 STM2666 STM1224 STM0972 STM1583 STM2875 STM2328 STM1269 mig-5 Gene First locusa TABLE 2. Salmonella operons with high expression during typhoid...”
- Adaptation of multilocus sequencing for studying variation within a major clone: evolutionary relationships of Salmonella enterica serovar Typhimurium
Hu, Genetics 2006 - “...A G to T G to A A to G C to T (s, STM1002) G to T (ns, STM1269) G to A G to A (nm, STM1858) T to C T (de) G to A A to T A (ie) A to G T to G C to T (ns, pbpC) C...”
- More robust detection of motifs in coexpressed genes by using phylogenetic information
Monsieurs, BMC bioinformatics 2006 - “...of six additional potential PmrA targets. Two of these six genes ( S. typhimurium ( STM1269 and ais ) did not contain an ortholog in E. coli or Y. pestis , and in the four remaining genes ( ybjG, ygiW, yijP and yegH ) the PmrA...”
- “...(pmrC), yfbE (pmrH), ugd ( udg, pmrE, pagA), yibD , ybjG ( mig-13 ) and STM1269 ( aroQ ) are known members of the PmrAB regulon that were confirmed in different experiments [ 23 , 34 - 38 ]. The last gene, yijP (STM4118) , was...”
- Identification and functional analysis of Salmonella enterica serovar Typhimurium PmrA-regulated genes
Tamayo, FEMS immunology and medical microbiology 2005 (PubMed)- “...of six new loci in the PmrA-PmrB regulon: STM1253, STM1269, STM4118, STM0459, STM3968 and STM4568. These loci did not affect the ability to grow in high iron...”
- “...products): STM1253, putative inner membrane protein; STM1269, putative chorismate mutase; STM4118, putative integral membrane protein; STM3968, uridine...”
- In silico identification and experimental validation of PmrAB targets in Salmonella typhimurium by regulatory motif detection
Marchal, Genome biology 2004 - “...were selected for biological validation: yibD (novel at the time of our analysis), aroQ ( STM1269 ), mig-13 and sseJ . aroQ and yibD were selected because a perfect repeat of the previously described PmrA half-site (CTTAAT [ 15 ]) was detected in their respective intergenic...”
- “..., lasT and ybdN. Promising targets only present in S. typhimurium and/or S. typhi were STM1269 ( aroQ ), STM1273 , sseJ and lpfA. Note that this listing is just based on an arbitrary selection criterion, that is, a preliminary PmrA motif consensus that will be...”
STY1852 putative chorismate mutase from Salmonella enterica subsp. enterica serovar Typhi str. CT18
Aligns to 29:99 / 181 (39.2%), covers 87.3% of PF01817, 67.2 bits
KPN_02920 bifunctional chorismate mutase P/prephenate dehydratase from Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Aligns to 11:89 / 386 (20.5%), covers 100.0% of PF01817, 66.9 bits
Ent638_3077 bifunctional chorismate mutase/prephenate dehydratase from Enterobacter sp. 638
Aligns to 21:99 / 396 (19.9%), covers 100.0% of PF01817, 66.9 bits
C7EXK8 prephenate dehydratase (EC 4.2.1.51); chorismate mutase (EC 5.4.99.5) from Escherichia coli (see 2 papers)
Aligns to 11:89 / 386 (20.5%), covers 100.0% of PF01817, 66.8 bits
PheA / b2599 fused chorismate mutase/prephenate dehydratase (EC 5.4.99.5; EC 4.2.1.51) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
pheA / P0A9J8 fused chorismate mutase/prephenate dehydratase (EC 5.4.99.5; EC 4.2.1.51) from Escherichia coli (strain K12) (see 16 papers)
CMPDT_ECOLI / P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12) (see paper)
CH_024084 P-protein; EC 4.2.1.51; EC 5.4.99.5 from Escherichia coli K12 (see paper)
b2599 fused chorismate mutase P/prephenate dehydratase from Escherichia coli str. K-12 substr. MG1655
NP_417090 fused chorismate mutase/prephenate dehydratase from Escherichia coli str. K-12 substr. MG1655
P0A9K0 Bifunctional chorismate mutase/prephenate dehydratase from Shigella flexneri
Aligns to 11:89 / 386 (20.5%), covers 100.0% of PF01817, 66.8 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate and the decarboxylation/dehydration of prephenate to phenylpyruvate.
catalytic activity: chorismate = prephenate (RHEA:13897)
catalytic activity: H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O (RHEA:21648)
subunit: Homodimer. - Genomic SELEX for Hfq-binding RNAs identifies genomic aptamers predominantly in antisense transcripts
Lorenz, Nucleic acids research 2010 - “...053 yebQ b1828 Antisense Predicted transporter 430 53 2 736 022 2 736 135 pheA b2599 Sense Fused chorismate mutase P/prephenate dehydratase 674 52 3 955 917 3 956 023 ilvC b3774 Sense Ketol-acid reductoisomerase, NAD(P)-binding 871 51 632 630 632 729 ybdH b0599 Sense Predicted...”
- Genome-scale analysis to the impact of gene deletion on the metabolism of E. coli: constraint-based simulation approach
Xu, BMC bioinformatics 2009 - “...b0908, b1260 b0914 b1098 b1136 b1261, b1262 s0001 b2827 b1263, b1264 b3648 b1693, b2329 s0001 b2599, b2600 b3389 SS AAM FM ACM HM CM genes b0928 b1415 b1415 b2019, b2020 b2750, b2751 b3941 b3608 b2021, b2022 b2752, b2762 b2023, b2024 b2763, b2764 b2025, b2026 b3607 SS...”
- Experimental and computational assessment of conditionally essential genes in Escherichia coli
Joyce, Journal of bacteriology 2006 - “...metF (b3941) metL (b3940) pabA (b3360) pabB (b1812) pheA (b2599) proA (b0243) proB (b0242) proC (b0386) serA (b2913) serB (b4388) serC (b0907) thrA (b0002) thrB...”
- Phenotypic screening of Escherichia coli K-12 Tn5 insertion libraries, using whole-genome oligonucleotide microarrays
Winterberg, Applied and environmental microbiology 2005 - “...pheA ilvA ilvC serB cmr melC yhjB argE b0073 b2599 b3772 b3774 b4388 b0842 b3008 b3520 b3957 81170 2735838 3953883 3956841 4622451 883043 3150839 3669108...”
- Interfering with different steps of protein synthesis explored by transcriptional profiling of Escherichia coli K-12
Sabina, Journal of bacteriology 2003 - “...b3460 b2020 b4024 b3517 b3509 b2019 b3856 b1260 b0074 b3829 b2599 b2215 b3196 b4208 b0431 b1263 b4016 b0720 b3458 0.04 0.07 0.08 0.08 0.08 0.09 0.11 0.11 0.11...”
- Combined, functional genomic-biochemical approach to intermediary metabolism: interaction of acivicin, a glutamine amidotransferase inhibitor, with Escherichia coli K-12
Smulski, Journal of bacteriology 2001 - “...b0099 b1740 b1224 b1221 b1186 b0560 b3169 b0957 b1638 b2599 b1130 b0931 b3164 b4226 b0950 b1830 b4201 b0467 b3300 b2679 b4245 b4244 b3749 b0497 b3041 b1427...”
- Redesigning the PheA domain of gramicidin synthetase leads to a new understanding of the enzyme's mechanism and selectivity.
Stevens, Biochemistry 2006 (PubMed)- GeneRIF: The wild-type enzyme binds phenylalanine in a kinetically selective manner; no other amino acids tested appeared to bind the enzyme in the early time frame examined.
- Computationally designed variants of Escherichia coli chorismate mutase show altered catalytic activity.
Lassila, Protein engineering, design & selection : PEDS 2005 (PubMed)- GeneRIF: Computationally predicted chorismate mutase mutant Ala32Ser shows increased catalytic efficiency.
- Metabolic engineering of Synechocystis sp. PCC 6803 for the improved production of phenylpropanoids
Kukil, Microbial cell factories 2024 - “..., encoding the chorismate mutase domain of the fused chorismate mutase/prephenate dehydratase from E.coli (UniProtKB- P0A9J8). The tyrC sequence fragment carried a 5 overhang of XbaI site and RBS* and a 3 overhang with a strep-tag sequence, BcuI and PstI (or BamHI in case of tyrB...”
- Functional Prediction of Biological Profile During Eutrophication in Marine Environment
Sbaoui, Bioinformatics and biology insights 2022 - “...P22259 Phosphoenolpyruvate carboxykinase PfkA P0A796 6-Phosphofructokinase Pgi P0A6T1 Glucose-6-phosphate isomerase Pgk P0A799 3-phosphoglycerate kinase PheA P0A9J8 Bifunctional: chorismate mutase P (N-terminal); prephenate dehydratase (C-terminal) PheL P0AD72 Phe operon leader peptide PheT P07395 Phenylalanine tRNA synthetase, beta-subunit PhoU P0A9K7 Phosphate-specific transport system accessory protein PKS B2HIL7 Polyketide...”
- Bacterial bifunctional chorismate mutase-prephenate dehydratase PheA increases flux into the yeast phenylalanine pathway and improves mandelic acid production
Reifenrath, Metabolic engineering communications 2018 - “...in Supplementary table S3 . To receive a feedback-resistant variant of PheA (Uniprot accession number P0A9J8) the mutations T304, G305K and Q306 ( Nelms et al., 1992 ) were introduced. The gene was codon-optimized according to Wiedemann and Boles (2008) and ordered as a String DNA...”
- Genomic and proteomic characterization of two strains of Shigella flexneri 2 isolated from infants' stool samples in Argentina
Torrez, BMC genomics 2022 - “...Argininosuccinate synthase OS= Shigella flexneri OX=623 GN= argG PE=3 SV=2 30.43 27 49.9 5.39 E P0A9K0 pheA Bifunctional chorismate mutase/prephenate dehydratase OS= Shigella flexneri OX=623 GN= pheA PE=3 SV=1 25.91 18 43.1 6.68 E Q83LN3 pncB Nicotinate phosphoribosyltransferase OS= Shigella flexneri OX=623 GN= pncB PE=3 SV=4...”
PS417_13205 isochorismate lyase from Pseudomonas simiae
Aligns to 14:91 / 102 (76.5%), covers 98.7% of PF01817, 66.8 bits
NE0335 Prephenate dehydratase (PDT):Chorismate mutase:ACT domain from Nitrosomonas europaea ATCC 19718
Aligns to 9:83 / 355 (21.1%), covers 100.0% of PF01817, 66.5 bits
CHMU_ENTAG / P42517 Monofunctional chorismate mutase; CM-F; EC 5.4.99.5 from Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans) (see 2 papers)
Aligns to 30:99 / 181 (38.7%), covers 84.8% of PF01817, 66.5 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate. May sequester with cyclohexadienyl dehydratase and an aromatic aminotransferase to form phenylalanine or phenylpyruvate.
catalytic activity: chorismate = prephenate (RHEA:13897)
subunit: Homodimer.
PMI0390 P-protein [includes: chorismate mutase and prephenate dehydratase] from Proteus mirabilis HI4320
Aligns to 10:88 / 385 (20.5%), covers 100.0% of PF01817, 66.4 bits
PA14_09220 salicylate biosynthesis protein PchB from Pseudomonas aeruginosa UCBPP-PA14
Aligns to 14:91 / 101 (77.2%), covers 98.7% of PF01817, 66.3 bits
CC1116 chorismate mutase, putative from Caulobacter crescentus CB15
Aligns to 36:113 / 121 (64.5%), covers 100.0% of PF01817, 66.2 bits
L8A208 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, chorismate mutase-isozyme 3 from Geobacillus sp. GHH01
Aligns to 10:87 / 360 (21.7%), covers 100.0% of PF01817, 66.0 bits
2h9dD / Q51507 Pyruvate-bound structure of the isochorismate-pyruvate lyase from pseudomonas aerugionsa (see paper)
Aligns to 14:91 / 100 (78.0%), covers 98.7% of PF01817, 65.6 bits
- Ligands: pyruvic acid; calcium ion (2h9dD)
pchB / Q51507 isochorismate pyruvate lyase (EC 4.2.99.21) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
PCHB_PSEAE / Q51507 Isochorismate pyruvate lyase; IPL; Chorismate mutase; CM; Salicylate biosynthesis protein; EC 4.2.99.21; EC 5.4.99.5 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 6 papers)
Q51507 isochorismate lyase (EC 4.2.99.21) from Pseudomonas aeruginosa (see 3 papers)
NP_252920 isochorismate-pyruvate lyase from Pseudomonas aeruginosa PAO1
PA4230 salicylate biosynthesis protein PchB from Pseudomonas aeruginosa PAO1
Aligns to 14:91 / 101 (77.2%), covers 98.7% of PF01817, 65.6 bits
- function: Involved in the incorporation of salicylate into the siderophore pyochelin. Catalyzes the elimination of the enolpyruvyl side chain from isochorismate to yield salicylate and pyruvate via a rare pericyclic hydrogen transfer mechanism from C2 to C5. PchB also catalyzes the nonphysiological Claisen rearrangement of chorismate to prephenate in which the pyruvylenol tail is transferred from a C3 ether linkage to a C1-C9 linkage.
catalytic activity: isochorismate = pyruvate + salicylate (RHEA:27874)
catalytic activity: chorismate = prephenate (RHEA:13897)
subunit: Dimer of dimers. - Unveiling the Microbiome Landscape: A Metagenomic Study of Bacterial Diversity, Antibiotic Resistance, and Virulence Factors in the Sediments of the River Ganga, India
Rout, Antibiotics (Basel, Switzerland) 2023 - “...NP_252919 pchC contigs_665 Rasulabad Ghat NP_252914 pchG contigs_665 Rasulabad Ghat NP_252915 pchF contigs_665 Rasulabad Ghat NP_252920 pchB contigs_665 Rasulabad Ghat NP_252917 pchR contigs_4; contigs_24665; contigs_67305; contigs_39 Triveni Sangam; Bagwan; sahidabad; Bagwan NP_249099 pilG Type IV pili (VF0082) ( Pseudomonas aeruginosa ) contigs_24665; contigs_67305; contigs_39 Bagwan; Sahidabad;...”
- A gene network-driven approach to infer novel pathogenicity-associated genes: application to Pseudomonas aeruginosa PAO1
De, mSystems 2023 - “...pscQ PA1694 9.9 0.046 9.23 2.5e-03 41 pscO PA1696 13.47 0.031 13.40 2.0e-03 42 pchB PA4230 15.07 0.11 17.00 5.0e-03 42 pchD PA4228 47.6 0.14 4.21 8.5e-03 42 pchE PA4226 36.82 0.025 3.74 2.2e-02 42 pchG PA4224 22.29 0.34 5.86 6.5e-03 Use of machine learning-based perturbome...”
- Pf4 Phage Variant Infection Reduces Virulence-Associated Traits in Pseudomonas aeruginosa
Tortuel, Microbiology spectrum 2022 - “...PchD Fur, RsmA, PchR 10.20 PA4229 pchC Pyochelin biosynthetic protein PchC Fur, RsmA, PchR 11.76 PA4230 pchB Salicylate biosynthesis protein PchB Fur, RsmA, PchR 12.50 PA4231 pchA Salicylate biosynthesis isochorismate synthase Fur, RsmA, PchR 10.53 Virulence/biofilm switch GAC pathway PA0905 rsmA RsmA AlgU, AlgR, RsmY, RsmZ...”
- Dynamic Adaptive Response of Pseudomonas aeruginosa to Clindamycin/Rifampicin-Impregnated Catheters
Sung, Antibiotics (Basel, Switzerland) 2021 - “...pilF Type IV pilus biogenesis protein PilF 0.56 PA4211 phzB1 Phenazine biosynthesis protein PhzB 1000.00 PA4230 pchB Isochorismate pyruvate-lyase 0.69 PA4525 pilA Type IV pilin PilA 1.21 PA4555 pilY2 Type IV fimbrial biogenesis protein PilY2 0.00 PA4953 motB Flagellar motor rotation protein MotB 15.08 PA5042 pilO...”
- “...PilF 0.18 PA4211 phzB1 Phenazine biosynthesis protein PhzB 1000.00 PA4226 pchE Dihydroaeruginoate synthetase PchE 0.00 PA4230 pchB Isochorismate pyruvate-lyase 0.75 PA4525 pilA Type IV pilin PilA 21.73 PA4550 fimU type 4 fimbrial biogenesis protein FimU 0.00 PA4553 pilX Type IV fimbrial biogenesis protein PilX 0.00 PA4953...”
- Transcriptional analysis and target genes discovery of Pseudomonas aeruginosa biofilm developed ex vivo chronic wound model
Tan, AMB Express 2021 - “...bet A 5.99 3.10 209.46 Choline dehydrogenase PA4225 pch F -6.37 1.61 141.55 Pyochelin synthetase PA4230 pch B 6.48 1.44 144.11 Isochorismate-pyruvate lyase PA4888 des B 6.94 1.23 160.81 Acyl-CoA desaturase PA2114 PA2114 7.02 4.57 631.58 Major facilitator superfamily transporter PA2111 PA2111 7.26 5.09 835.52 Hypothetical...”
- Histamine: A Bacterial Signal Molecule
Krell, International journal of molecular sciences 2021 - “...synthetase PA4228 pchD 4.1 Pyochelin biosynthesis protein PchD PA4229 pchC 3.6 Pyochelin biosynthetic protein PchC PA4230 pchB 2.7 Salicylate biosynthesis protein PchB PA4231 pchA 2.3 Salicylate biosynthesis isochorismate synthase PA4687 hitA 3.3 Ferric iron-binding periplasmic protein HitA Iron transport [ 32 ] PA4688 hitB 3.2 Iron...”
- Increased ParB level affects expression of stress response, adaptation and virulence operons and potentiates repression of promoters adjacent to the high affinity binding sites parS3 and parS4 in Pseudomonas aeruginosa
Kawalek, PloS one 2017 - “...-2,9 -2,3 5 pyochelin biosynthesis protein PchD PA4229 pchC -2,2 5 pyochelin biosynthetic protein PchC PA4230 pchB -2,4 -2,1 6 salicylate biosynthesis protein PchB PA4231 pchA -2,5 -2,3 5 salicylate biosynthesis isochorismate synthase PA2384 -3,0 6 transcriptional regulator PA2403 -3,1 6 hypothetical protein PA2406 -2,7 -3,2...”
- “...PA4229 , PA4231 ) from Cluster 5 and three genes ( PA4224 , PA4225 , PA4230 ) from Cluster 6 are involved in the biosynthesis and transport of a siderophore, pyochelin. Pyochelin synthesis in P . aeruginosa cells is positively regulated by the transcriptional regulator PA2384...”
- Effect of efflux pump inhibition on Pseudomonas aeruginosa transcriptome and virulence
Rampioni, Scientific reports 2017 - “...2.26 pyochelinsynthetase PA4226 pchE 2.06 dihydroaeruginoic acid synthetase PA4228 pchD 2.09 pyochelin biosynthesis protein PchD PA4230 pchB 2.69 salicylate biosynthesis protein PchB PA4468 sodM 11.65 superoxide dismutase PA4470 fumC1 8.96 fumaratehydratase PA4708 phuT 3.17 heme-transport protein, PhuT PA4709 phuS 3.37 PhuS PA4710 phuR 4.65 heme/hemoglobin uptake...”
- New Mouse Model for Chronic Infections by Gram-Negative Bacteria Enabling the Study of Anti-Infective Efficacy and Host-Microbe Interactions
Pletzer, mBio 2017 - “...II secretion system substrate Lipase A 10.4 chpE (PA0417) Probable chemotaxis protein Chemotaxis 8.2 pchB (PA4230) Pyochelin siderophore biosynthesis Iron uptake 8.2 exsA (PA1713) Type III secretion transcriptional activator Type III secretion 8.0 mucC (PA0765) Positive regulator for alginate biosynthesis Exopolysaccharide 6.8 fliI (PA1104) Flagellum-specific ATP...”
- More
A8FGB2 Chorismate mutase from Bacillus pumilus (strain SAFR-032)
Aligns to 10:87 / 358 (21.8%), covers 100.0% of PF01817, 65.6 bits
PF1701 chorismate mutase from Pyrococcus furiosus DSM 3638
Q8U098 Chorismate mutase from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Aligns to 8:75 / 76 (89.5%), covers 98.7% of PF01817, 64.7 bits
HPG27_270 chorismate mutase from Helicobacter pylori G27
Aligns to 13:88 / 96 (79.2%), covers 100.0% of PF01817, 64.4 bits
BPSS0582 salicylate biosynthesis protein from Burkholderia pseudomallei K96243
Aligns to 14:91 / 101 (77.2%), covers 98.7% of PF01817, 64.2 bits
- Interrogation of the Burkholderia pseudomallei genome to address differential virulence among isolates
Challacombe, PloS one 2014 - “...synthase (EC 5.4.4.2)# Ubiquinone biosynthesis, Biosynthesis of siderophore group nonribosomal peptides, Biosynthesis of secondary metabolites BPSS0582 isochorismate-pyruvate lyase (EC 4.2.99.21) Siderophore biosynthesis BPSS0583 pyochelin biosynthetic protein PchC (EC 3.1.2.-) Siderophore biosynthesis BPSS0584 salicyl-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58)# Siderophore biosynthesis BPSS0586 pyochelin synthetase Siderophore biosynthesis BPSS0587...”
CE140_03015 isochorismate lyase from Pseudomonas thivervalensis
Aligns to 19:96 / 106 (73.6%), covers 98.7% of PF01817, 64.0 bits
SO_1367 chorismate mutase from Shewanella oneidensis MR-1
Aligns to 11:89 / 671 (11.8%), covers 100.0% of PF01817, 63.9 bits
VP0555 chorismate mutase/prephenate dehydratase from Vibrio parahaemolyticus RIMD 2210633
Aligns to 12:91 / 392 (20.4%), covers 100.0% of PF01817, 63.5 bits
JS608_03349, JTE87_00934 bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase from Bacillus amyloliquefaciens
A7Z7R9 3-deoxy-7-phosphoheptulonate synthase from Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / LMG 26770 / FZB42)
Aligns to 10:87 / 358 (21.8%), covers 100.0% of PF01817, 63.5 bits
- Multi-Omics Characterization of Host-Derived Bacillus spp. Probiotics for Improved Growth Performance in Poultry
Susanti, Frontiers in microbiology 2021 - “...probiotic Bacillus spp. Annotation Gene EC number PTA-84 PTA-85 PTA-86 Protein AroA(G) aro A 5.4.99.5 JS608_03349 JTE87_00934 JS609_02973 3-phosphoshikimate 1-carboxyvinyltransferase 1 aro A1 2.5.1.19 JS608_02738 JTE87_01546 JS609_02206 3-dehydroquinate synthase aro B 4.2.3.4 JS608_02748 JTE87_01536 JS609_02216 Chorismate synthase aro C 4.2.3.5 JS608_02749 JTE87_01535 JS609_02217 3-dehydroquinate dehydratase aro...”
- “...Bacillus spp. Annotation Gene EC number PTA-84 PTA-85 PTA-86 Protein AroA(G) aro A 5.4.99.5 JS608_03349 JTE87_00934 JS609_02973 3-phosphoshikimate 1-carboxyvinyltransferase 1 aro A1 2.5.1.19 JS608_02738 JTE87_01546 JS609_02206 3-dehydroquinate synthase aro B 4.2.3.4 JS608_02748 JTE87_01536 JS609_02216 Chorismate synthase aro C 4.2.3.5 JS608_02749 JTE87_01535 JS609_02217 3-dehydroquinate dehydratase aro D...”
- S-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria
Chi, Antioxidants & redox signaling 2013 - “...SerA AroA AroE GuaB YumC ThiI CotE SufA A7Z657 A7Z7R9 A7Z612 A7Z0D1 A7Z8C1 A7Z7N3 A7Z4X4 F4EAC4 AroA Tuf MetI A8FGB2 B4AN33 B4AF61 B4ANL1 LuxS YwaA A8FIT1 B....”
ASF14_15595 chorismate mutase from Sphingomonas sp. Leaf257
Aligns to 17:94 / 102 (76.5%), covers 98.7% of PF01817, 63.4 bits
CA265_RS11635 chorismate mutase (EC 5.4.99.5); 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) from Pedobacter sp. GW460-11-11-14-LB5
Aligns to 279:357 / 379 (20.8%), covers 98.7% of PF01817, 63.3 bits
- mutant phenotype: Important for fitness in most defined media. The N-terminal DHAP region is only 36% identical to the Pyrococcus enzyme (Q8U0A9) but the active site and substrate-binding residues are conserved. The C-terminal chorismate mutase region is diverged but hits PF01817 and no other chorismate mutase was found in the genome except for a paralog that has little phenotype (CA264_13110)
P39912 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54); chorismate mutase (EC 5.4.99.5) from Bacillus subtilis (see paper)
BSU29750 bifunctional chorismate mutase type II-isozyme 3 (regulatory domain); 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Bacillus subtilis subsp. subtilis str. 168
JS609_02973 bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase from Bacillus subtilis
Aligns to 10:87 / 358 (21.8%), covers 100.0% of PF01817, 63.1 bits
- Insights into the Structure and Protein Composition of Moorella thermoacetica Spores Formed at Different Temperatures
Malleck, International journal of molecular sciences 2022 - “...BSU40360 moth_0925 Q2RJZ7 Small acid-soluble spore protein, alpha/beta type SspA Protection of spore DNA Core BSU29750 CD2688 moth_0806 Q2RKB5 Small, acid-soluble spore protein, alpha/beta family SspF Protection of the spore DNA Core BSU24210 moth_1875 Q2RHB4 Small, acid-soluble spore protein SASP Small, acid-soluble spore protein Core CPR1870...”
- The Blueprint of a Minimal Cell: MiniBacillus
Reuß, Microbiology and molecular biology reviews : MMBR 2016 - “...Syn3.0c EC no. PDB accession no. Organismd Function(s) BSU29750 No 2.5.1.54 3NVT L. monocytogenes aroB aroC aroD aroE BSU22700 BSU23080 BSU25660 BSU22600 No No...”
- S-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria
Chi, Antioxidants & redox signaling 2013 - “...A7ZAR2 PpaC B. amyloliquefaciens A7Z3U1 MetE YphP*,# AroA P39912 P54170 SerA* P35136 GuaB PpaC* P37487 P21879 YxjG* P42318 Tuf MetE* P80877 P33166 OhrR* Protein...”
- A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study
Albaum, Proteome science 2011 - “...<0.000001 <0.000001 1692 P37494 0.000244 >0.99 >0.99 0.932235 241 P32399 0.000267 >0.99 >0.99 0.045315 388 P39912 0.000327 >0.99 >0.99 0.002252 878 P42971 0.000473 >0.99 >0.99 0.032372 475 P21472 0.000594 >0.99 >0.99 0.99 357 P21464 0.000613 >0.99 <0.000001 0.000473 1272 P71021 0.000703 >0.99 >0.99 0.99 173 O31567...”
- “...>0.99 0.000693 751 O32157 0.000035 0.016583 <0.000001 0.000796 957 P21467 0.015346 >0.99 0.000001 0.001040 781 P39912 0.000327 >0.99 >0.99 0.002252 878 P23446 >0.99 >0.99 <0.000001 0.002510 451 P49785 0.039250 >0.99 0.000245 0.002557 536 O31567 0.001315 >0.99 0.000289 0.002616 613 P46922 0.069376 >0.99 0.000162 0.002782 581 Q01625...”
- Multi-Omics Characterization of Host-Derived Bacillus spp. Probiotics for Improved Growth Performance in Poultry
Susanti, Frontiers in microbiology 2021 - “...spp. Annotation Gene EC number PTA-84 PTA-85 PTA-86 Protein AroA(G) aro A 5.4.99.5 JS608_03349 JTE87_00934 JS609_02973 3-phosphoshikimate 1-carboxyvinyltransferase 1 aro A1 2.5.1.19 JS608_02738 JTE87_01546 JS609_02206 3-dehydroquinate synthase aro B 4.2.3.4 JS608_02748 JTE87_01536 JS609_02216 Chorismate synthase aro C 4.2.3.5 JS608_02749 JTE87_01535 JS609_02217 3-dehydroquinate dehydratase aro D 4.2.1.10...”
CAC0897 Fusion: chorismate mutase and shikimate 5-dehydrogenase from Clostridium acetobutylicum ATCC 824
Aligns to 8:85 / 367 (21.3%), covers 98.7% of PF01817, 62.8 bits
- Complex and extensive post-transcriptional regulation revealed by integrative proteomic and transcriptomic analysis of metabolite stress response in Clostridium acetobutylicum
Venkataramanan, Biotechnology for biofuels 2015 - “...CAC3713 - hypothetical protein, CAC3097 - RpsI ribosomal protein, CAC2641 - trigger factor) and five (CAC0897 - aro, CAC3171 - LeuB, CAC3243 - chemotaxis protein, CAC0827 - fructose bisphosphate aldolase, CAC0972 - isocitrate dehydrogenase) proteins were found at medium and high stress, respectively, while two (CAC3598...”
- “...Gene Function 5UTR RBS score CAC3713 Hypothetical protein 96 12.87 CAC2641 Trigger factor 33 12.87 CAC0897 Aro 13 9.25 CAC3171 LeuB 30 7.68 CAC3243 Chemotaxis protein 15 12.38 CAC3598 Rubrerythrin 96 12.87 Genes are in italics to indicate their differential proteomic upregulation Table 5 Key genes...”
- Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis
Senger, Biotechnology and bioengineering 2008 - “...List 5: CAC1825, CAC0390, CAC0391, CAC3348/CAC0578. List 6: CAC0737/CAC1001/CAC1819/CAC2832/CAC0764/CAC1673/CAC1674. List 7: (from DAHP) CAC0894, CAC0713/CAC0899, CAC0897, CAC0898, CAC0895, CAC0896. List 8: CAC2680, CAC0944, CAC1348, CAC1730, CAC0726, CAC0819/CAC3221. List 9: CAC0936, CAC0943, CAC0942, CAC0940, CAC0939, CAC0938, CAC1369, CAC2727, CAC0937. List 10: CAC3162/CAC3163, CAC3161, CAC3159, CAC3160, CAC3157/CAC3158. List...”
- Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum
Alsaker, Journal of bacteriology 2005 - “...are grouped in clusters t2 (CAC0896 and CAC0898), t3 (CAC0897), and t4 (CAC0892 to CAC0895 and CAC899), and the tryptophan formation genes are grouped in...”
2gtvX / Q57696 Nmr structure of monomeric chorismate mutase from methanococcus jannaschii (see paper)
Aligns to 9:95 / 104 (83.7%), covers 100.0% of PF01817, 62.4 bits
- Ligand: 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid (2gtvX)
CE140_05235 isochorismate lyase from Pseudomonas thivervalensis
Aligns to 14:91 / 102 (76.5%), covers 98.7% of PF01817, 62.3 bits
ELZ14_13330 isochorismate lyase from Pseudomonas brassicacearum
Aligns to 16:93 / 103 (75.7%), covers 98.7% of PF01817, 62.0 bits
lmo1600 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Listeria monocytogenes EGD-e
Aligns to 10:87 / 361 (21.6%), covers 100.0% of PF01817, 61.3 bits
swp_3758 Chorismate mutase, gammaproteobacteria from Shewanella piezotolerans WP3
Aligns to 11:89 / 658 (12.0%), covers 100.0% of PF01817, 60.5 bits
- Condition-Specific Molecular Network Analysis Revealed That Flagellar Proteins Are Involved in Electron Transfer Processes of Shewanella piezotolerans WP3
Ding, Genetics research 2021 - “...acid oxidation complex subunit (swp_3139), 2-isopropylmalate synthase (swp_2213), isopropylmalate isomerase small subunit (swp_2216), chorismate mutase (swp_3758), Na + -transporting methylmalonyl-CoA/oxaloacetate decarboxylase subunit (swp_1380), sodium pump decarboxylase subunit (swp_1378), and malate dehydrogenase (swp_0481). These results are consistent with previous KEGG enrichment results, indicating that cell metabolism undergoes...”
- “...swp_3139 swp_4352 swp_4934 5 swp_5110 swp_2213 swp_0430 swp_2897 6 swp_5111 swp_2216 swp_2139 swp_0501 7 swp_1508 swp_3758 swp_0854 swp_0495 8 swp_5087 swp_1380 swp_4933 swp_0486 9 swp_2950 swp_1378 swp_1531 swp_4145 10 swp_0586 swp_0481 swp_1521, swp_1527 swp_4144 Note: as many proteins have the same degree value, we used the...”
PAPB_STRPR / P72541 4-amino-4-deoxychorismate mutase; ADC mutase; EC 5.4.99.67 from Streptomyces pristinaespiralis (see paper)
P72541 4-amino-4-deoxychorismate mutase (EC 5.4.99.67) from Streptomyces pristinaespiralis (see paper)
Aligns to 26:104 / 129 (61.2%), covers 98.7% of PF01817, 59.8 bits
plu1265 P-protein [includes: chorismate mutase (CM); prephenate dehydratase (PDT)] from Photorhabdus luminescens subsp. laumondii TTO1
Aligns to 10:88 / 385 (20.5%), covers 100.0% of PF01817, 59.3 bits
CPI83_19930 chorismate mutase family protein from Rhodococcus sp. H-CA8f
Aligns to 71:149 / 174 (45.4%), covers 98.7% of PF01817, 58.4 bits
P43900 Bifunctional chorismate mutase/prephenate dehydratase from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
HI1145 chorismate mutase / prephenate dehydratase (pheA) from Haemophilus influenzae Rd KW20
Aligns to 9:89 / 385 (21.0%), covers 100.0% of PF01817, 58.3 bits
- Prephenate dehydratase from the aphid endosymbiont (Buchnera) displays changes in the regulatory domain that suggest its desensitization to inhibition by phenylalanine
Jiménez, Journal of bacteriology 2000 - “...(E. herbicola, Q02286), PDTHAEIN (Haemophilus influenzae, P43900), PDTATHA1 (Arabidopsis thaliana, O22241), PDTATHA2 (A. thaliana, AAD30242), PDTATHA3 (A....”
- Genome-scale approaches to identify genes essential for Haemophilus influenzae pathogenesis
Wong, Frontiers in cellular and infection microbiology 2012 - “...Rd Locus Function Attenuated in vivo /serum Gene/protein induced in vivo /sputum AMINO ACID METABOLISM HI1145 pheA Chorismate mutase/prephenate dehydratase a HI1387 trpE Anthranilate synthase component I a HI1388 trpG Anthranilate synthase component II a HI1389 trpD Anthranilate phosphoribosyltransferase a HI1389.1 trpC Bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate...”
SS1G_14320 predicted protein from Sclerotinia sclerotiorum 1980 UF-70
Aligns to 74:153 / 160 (50.0%), covers 98.7% of PF01817, 57.8 bits
Kkor_1584 Prephenate dehydratase from Kangiella koreensis DSM 16069
Aligns to 12:90 / 398 (19.8%), covers 100.0% of PF01817, 57.4 bits
cmlD / F2RB77 4-amino-4-deoxychorismate mutase (EC 5.4.99.67) from Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745) (see 2 papers)
papB / BAD21142.1 4-amino-4-deoxychorismate mutase from Streptomyces venezuelae (see paper)
Aligns to 11:89 / 103 (76.7%), covers 98.7% of PF01817, 56.5 bits
3nvtA / Q8Y6T2 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
Aligns to 3:75 / 345 (21.2%), covers 100.0% of PF01817, 54.5 bits
- Ligand: manganese (ii) ion (3nvtA)
M271_43345 chorismate mutase from Streptomyces rapamycinicus NRRL 5491
Aligns to 55:125 / 213 (33.3%), covers 86.1% of PF01817, 53.1 bits
CC2222 chorismate mutase, putative from Caulobacter crescentus CB15
Aligns to 31:110 / 281 (28.5%), covers 98.7% of PF01817, 52.9 bits
LSA1662 Hypothetical protein from Lactobacillus sakei subsp. sakei 23K
Aligns to 7:83 / 95 (81.1%), covers 97.5% of PF01817, 52.9 bits
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...protein 1.7 D LSA1649 lsa1649 Hypothetical extracellular protein precursor -0.5 LSA1659 lsa1659 Hypothetical protein -0.5 LSA1662 lsa1662 Hypothetical protein -1.0 -0.6 -0.7 LSA1663 lsa1663 Hypothetical protein -0.8 LSA1678 lsa1678 Hypothetical protein -0.6 LSA1680 lsa1680 Hypothetical protein -0.6 LSA1716 lsa1716 Hypothetical protein -0.5 LSA1822 lsa1822 Hypothetical protein...”
PSPTO_0357 chorismate mutase from Pseudomonas syringae pv. tomato str. DC3000
Aligns to 31:101 / 185 (38.4%), covers 86.1% of PF01817, 51.6 bits
SCAB_58971 putative chorismate mutase from Streptomyces scabiei 87.22
Aligns to 56:126 / 218 (32.6%), covers 87.3% of PF01817, 51.4 bits
BBMN68_1136 chorismate mutase from Bifidobacterium longum subsp. longum BBMN68
Aligns to 35:114 / 129 (62.0%), covers 94.9% of PF01817, 51.2 bits
2ao2A / P9WIB9 The 2.07 angstrom crystal structure of mycobacterium tuberculosis chorismate mutase reveals unexpected gene duplication and suggests a role in host-pathogen interactions (see paper)
Aligns to 6:76 / 165 (43.0%), covers 87.3% of PF01817, 50.0 bits
- Ligand: sulfate ion (2ao2A)
SCMU_MYCTU / P9WIB9 Secreted chorismate mutase; CM; *MtCM; EC 5.4.99.5 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 5 papers)
NP_216401 chorismate mutase from Mycobacterium tuberculosis H37Rv
NP_216401, Rv1885c chorismate mutase from Mycobacterium tuberculosis H37Rv
Aligns to 40:110 / 199 (35.7%), covers 87.3% of PF01817, 48.9 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate. May play some role in the pathogenicity.
catalytic activity: chorismate = prephenate (RHEA:13897)
subunit: Homodimer. - Novel exported fusion enzymes with chorismate mutase and cyclohexadienyl dehydratase activity: Shikimate pathway enzymes teamed up in no man's land
Stocker, The Journal of biological chemistry 2023 - “...sequence of the AroQ enzyme MtCM ( 19 ), including its N-terminal secretion signal (UniProt: P9WIB9 ), was taken as input for a BLASTP search in the NCBI database ( 34 ). Besides other monofunctional exported CMs with >80% sequence coverage, a few search hits stood...”
- “...by BLASTP ( 76 ) using the fully translated open reading frame Rv1885c (MtCM; UniProt: P9WIB9 ) ( 19 ) including the signal peptide. The bifunctional fusion enzymes were identified by 50% query coverage but with high sequence similarity and identity scores. Only search hits with...”
- A secreted form of chorismate mutase (Rv1885c) in Mycobacterium bovis BCG contributes to pathogenesis by inhibiting mitochondria-mediated apoptotic cell death of macrophages.
Lee, Journal of biomedical science 2023 - GeneRIF: A secreted form of chorismate mutase (Rv1885c) in Mycobacterium bovis BCG contributes to pathogenesis by inhibiting mitochondria-mediated apoptotic cell death of macrophages.
- Complex Formation between Two Biosynthetic Enzymes Modifies the Allosteric Regulatory Properties of Both: AN EXAMPLE OF MOLECULAR SYMBIOSIS.
Blackmore, The Journal of biological chemistry 2015 - GeneRIF: DAH7PS and chorismate mutase provide exquisite control of aromatic amino acid biosynthesis, not only controlling flux into the start of the pathway, but also directing the pathway intermediate chorismate into either Phe/Tyr or Trp biosynthesis.
- Molecular dynamics simulation of the last step of a catalytic cycle: product release from the active site of the enzyme chorismate mutase from Mycobacterium tuberculosis.
Choutko, Protein science : a publication of the Protein Society 2012 - GeneRIF: Studied the product release from the active site of the Mycobacterium tuberculosis chorismate mutase using molecular dynamics simulation.
- The two chorismate mutases from both Mycobacterium tuberculosis and Mycobacterium smegmatis: biochemical analysis and limited regulation of promoter activity by aromatic amino acids.
Schneider, Journal of bacteriology 2008 - GeneRIF: Genes from M. tuberculosis are constitutively expressed or subjected to minor regulation by aromatic amino acids levels, especially tryptophan.
- Preliminary X-ray crystallographic analysis of the secreted chorismate mutase from Mycobacterium tuberculosis: a tricky crystallization problem solved.
Krengel, Acta crystallographica. Section F, Structural biology and crystallization communications 2006 - GeneRIF: Secreted chorismate mutase from Mycobacterium tuberculosis a 37.2 kDa dimeric protein belonging to AroQ(gamma) subclass of mutases, crystallized. Asymmetric unit contains 1 biological dimer, with 167 amino acids per protomer.
- The 2.15 A crystal structure of Mycobacterium tuberculosis chorismate mutase reveals an unexpected gene duplication and suggests a role in host-pathogen interactions.
Qamra, Biochemistry 2006 (PubMed)- GeneRIF: chorismate mutase might have a role to play in host-pathogen interactions, making it an important target for designing inhibitor molecules against the deadly pathogen
- Biochemical and structural characterization of the secreted chorismate mutase (Rv1885c) from Mycobacterium tuberculosis H37Rv: an *AroQ enzyme not regulated by the aromatic amino acids.
Kim, Journal of bacteriology 2006 - GeneRIF: Our investigation on the effect of phenylalanine, tyrosine, and tryptophan on *chorismate mutase (MtCM) shows that MtCM is not regulated by the aromatic amino acids. The X-ray structure of MtCM does not have an allosteric regulatory site. [MtCM]
- Purified recombinant hypothetical protein coded by open reading frame Rv1885c of Mycobacterium tuberculosis exhibits a monofunctional AroQ class of periplasmic chorismate mutase activity.
Prakash, The Journal of biological chemistry 2005 (PubMed)- GeneRIF: The Rv1885c-encoded chorismate mutase showed allosteric regulation by pathway-specific as well as cross-pathway-specific ligands, as evident from proteolytic cleavage protection and enzyme assays.
- Transposon sequencing reveals metabolic pathways essential for Mycobacterium tuberculosis infection
Block, PLoS pathogens 2024 - “...by chorismate mutase [ 45 ]. Two M . tuberculosis proteins have chorismate mutase activity (Rv1885c and Rv0948c), but Rv1885c is secreted while Rv0948c is cytosolic [ 47 49 ]. Only rv0948c is a M-ES gene [ 11 ], suggesting that it functions as the chorismate...”
- “...A , Hasnain S E . Purified recombinant hypothetical protein coded by open reading frame Rv1885c of Mycobacterium tuberculosis exhibits a monofunctional AroQ class of periplasmid chorismate mutase activity . J Biol Chem . 2005 ; 280 : 19641 8 . 15737998 48 Sasso S ,...”
- The implication of dendritic cells in lung diseases: Immunological role of toll-like receptor 4
Xuan, Genes & diseases 2024 - A secreted form of chorismate mutase (Rv1885c) in Mycobacterium bovis BCG contributes to pathogenesis by inhibiting mitochondria-mediated apoptotic cell death of macrophages
Lee, Journal of biomedical science 2023 - “...1423-0127 BioMed Central London 10729386 38110948 988 10.1186/s12929-023-00988-2 Research A secreted form of chorismate mutase (Rv1885c) in Mycobacterium bovis BCG contributes to pathogenesis by inhibiting mitochondria-mediated apoptotic cell death of macrophages Lee Mi-Hyun 1 2 3 Kim Hye Lin 1 5 Seo Hyejun 1 5 6...”
- “...defense system. The secretory form of the chorismate mutase of M. tuberculosis (TBCM, encoded by Rv1885c) is assumed to play a key role in the pathogenesis of TB; however, the mechanism remains unknown. Methods A tbcm deletion mutant (B tbcm ) was generated by targeted gene...”
- Novel exported fusion enzymes with chorismate mutase and cyclohexadienyl dehydratase activity: Shikimate pathway enzymes teamed up in no man's land
Stocker, The Journal of biological chemistry 2023 - “...domain were discovered by BLASTP ( 76 ) using the fully translated open reading frame Rv1885c (MtCM; UniProt: P9WIB9 ) ( 19 ) including the signal peptide. The bifunctional fusion enzymes were identified by 50% query coverage but with high sequence similarity and identity scores. Only...”
- Potential of Mycobacterium tuberculosis chorismate mutase (Rv1885c) as a novel TLR4-mediated adjuvant for dendritic cell-based cancer immunotherapy
Jeong, Oncoimmunology 2022 - “...10.1080/2162402X.2021.2023340 2023340 Version of Record Research Article Research Article Potential of Mycobacterium tuberculosis chorismate mutase (Rv1885c) as a novel TLR4-mediated adjuvant for dendritic cell-based cancer immunotherapy H. JEONG ET AL. ONCOIMMUNOLOGY https://orcid.org/0000-0001-8416-7091 Jeong Hyein a b c d e f Lee So-Young a c d e...”
- “...strong anticancer immune response is needed. Here, we investigated the potential of chorismate mutase (TBCM, Rv1885c), a putative Mycobacterium tuberculosis (TB) virulence factor, as an immunoadjuvant in DC-based tumor immunotherapy. First, we found that TBCM functionally activated DCs by upregulating costimulatory molecules, increasing the secretion of...”
- Indole: A promising scaffold for the discovery and development of potential anti-tubercular agents
Bajad, Current research in pharmacology and drug discovery 2022 - “...of the essential amino acids phenylalanine and tyrosine. The secretory form, Mtb CM, encoded by Rv1885c , is believed to actively participate in the pathogenesis of TB. Therefore, the inhibition of CM may directly hinder the supply of nutrients to the organism ( Khanapur etal., 2017...”
- The pathogenic mechanism of Mycobacterium tuberculosis: implication for new drug development
Yan, Molecular biomedicine 2022 - “...Description 1 pks13 (Rv3800c) [ 329 ] Polyketide synthase Pks13 Synthesis of mycolic acid 2 Rv1885c Chorismate mutase (Mtb CM) [ 330 ] Synthesis of shikimate (rearrange chorismate to prephenate) 3 alr (Rv3423c) Alanine racemase [ 331 ] Synthesis of cell wall (racemase L-alanine into D-alanine)...”
- Rational Design of Biosafety Level 2-Approved, Multidrug-Resistant Strains of Mycobacterium tuberculosis through Nutrient Auxotrophy
Vilchèze, mBio 2018 - “...Probable short-chain-type dehydrogenase Rv1883c 21332312133692 10 Conserved hypothetical protein rpfC 21337312134261 3 Probable resuscitation-promoting factor Rv1885c 21342732134872 7 Conserved hypothetical protein fbpB 21348902135867 1 Secreted antigen 85-B Rv1887 21362582137400 10 Hypothetical protein Rv1888c 21375192138079 10 Possible transmembrane protein Rv1888A 21384442138617 3 Conserved hypothetical protein Rv1889c 21386612139017...”
- More
SCMU_MYCS2 / A0QU81 Secreted chorismate mutase; CM; EC 5.4.99.5 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
Aligns to 28:97 / 181 (38.7%), covers 86.1% of PF01817, 47.7 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate. May play some role in the pathogenicity.
catalytic activity: chorismate = prephenate (RHEA:13897)
subunit: Homodimer.
Q2SY64 chorismate mutase (EC 5.4.99.5) from Burkholderia thailandensis (see paper)
Aligns to 40:110 / 202 (35.1%), covers 88.6% of PF01817, 47.7 bits
O30012 prephenate dehydrogenase (EC 1.3.1.12); prephenate dehydratase (EC 4.2.1.51); chorismate mutase (EC 5.4.99.5) from Archaeoglobus fulgidus (see paper)
AF0227 chorismate mutase/prephenate dehydratase (pheA) from Archaeoglobus fulgidus DSM 4304
Aligns to 270:345 / 620 (12.3%), covers 100.0% of PF01817, 47.2 bits
- Prephenate dehydratase from the aphid endosymbiont (Buchnera) displays changes in the regulatory domain that suggest its desensitization to inhibition by phenylalanine
Jiménez, Journal of bacteriology 2000 - “...pombe, O14361), PDTARCFU (Archaeoglobus fulgidus, O30012), PDTAQUAE (Aquifex aeolicus, O67085), PDTXANCA (Xanthomonas campestris, O87954), PDTPSEAER (P....”
- Characterization of a key trifunctional enzyme for aromatic amino acid biosynthesis in Archaeoglobus fulgidus
Lim, Extremophiles : life under extreme conditions 2009 (PubMed)- “...proteins or fusions combining two activities. Gene locus AF0227 of Archaeoglobus fulgidus is predicted to encode a fusion protein, AroQ, containing all three...”
- “...to be particularly interesting since its gene at locus AF0227 is predicted to encode a protein containing three domains harboring putative CM, PDT, and PDHG...”
- Evolution of gene fusions: horizontal transfer versus independent events
Yanai, Genome biology 2002 - “...of the fused gene (Figure 4a,4b ). The single archaeal fusion, the Arachaeoglobus fulgidus protein AF0227, belongs to one of these clusters and shows a strongly supported affinity with the ortholog from the hyperthermophilic bacterium Thermotoga maritima . (Figure 4a,4b ). Given the broad distribution of...”
- “...(COG1605); (b) prephenate dehydratase (COG0077). Protein designations are as in Figure 2 . The protein AF0227 contains a prephenate dehydrogenase domain in addition to the chorismate mutase and prephenate dehydratase domains. Figure 5 Phylogenetic trees for fusion-linked COGs: and subunits of acetyl-CoA carboxylase. (a) subunit (domain)...”
- Identification of 86 candidates for small non-messenger RNAs from the archaeon Archaeoglobus fulgidus
Tang, Proceedings of the National Academy of Sciences of the United States of America 2002 - “...() AF1049 () AF1987 () AF1017 () AF0790 () AF2390 () AF0227 () AF0896 () AF0701 () AF1489 () AF1277 () AF1444 () AF0208 () AF0595 () AF0592 () AF0597 () AF2236...”
BCAL3004 putative chorismate mutase from Burkholderia cenocepacia J2315
Aligns to 35:105 / 197 (36.0%), covers 87.3% of PF01817, 47.1 bits
- Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS
Wong, Frontiers in microbiology 2016 - “...involved in the conversion of chorismate to phenylalanine and tyrosine ( pheA , BCAL2953, and BCAL3004) as well as tryptophan ( trpA, trpC, trpD , and trpF ). We suspect that trp mutants may be tryptophan auxotrophs and are unable to grow in a tryptophan-depleted environment....”
- “...Lysine biosynthesis dapF, BCAS0211 Phenylalanine, tyrosine, and tryptophan biosynthesis phhA, aroK, trpC, trpD, BCAL2953, pheA, BCAL3004, aroE, trpF, trpA, BCAS0366 Valine, leucine, and isoleucine biosynthesis ilvA, ilvI, leuD1, leuB, BCAS0025 FIGURE 6 Schematic diagram of KEGG-derived phenylalanine, tyrosine and tryptophan biosynthesis pathways. Boxes indicate key products/intermediates....”
XC_3676 chorismate mutase/prephenate dehydratase from Xanthomonas campestris pv. campestris str. 8004
Aligns to 35:105 / 189 (37.6%), covers 87.3% of PF01817, 46.9 bits
MAB_1053c Conserved hypothetical protein (chorismate mutase?) from Mycobacterium abscessus ATCC 19977
Aligns to 20:85 / 92 (71.7%), covers 69.6% of PF01817, 46.8 bits
- Dissecting the antibacterial activity of oxadiazolone-core derivatives against Mycobacterium abscessus
Madani, PloS one 2020 - “...MAB_0520 38.811 Rv3626c - uncharacterized protein - MAB_0684c 26.813 Rv0774c CF Hypothetical extracellular esterase CW/CP MAB_1053c 10.305 Rv0948c In vitro growth WCL Chorismate mutase IM/R MAB_1675 28.418 Rv2362c In vitro growth CW Possible DNA repair protein RecO IP MAB_2366 33.804 Rv1701 - Probable integrase RP MAB_2477c...”
- “...them, MAB_1675, the probable DNA repair protein RecO ( i . e ., Rv2362c), and MAB_1053c ( i . e ., Rv0948c) a putative chorismate mutase possibly involved in phenylalanine, tyrosine and tryptophan biosynthesis, are annotated as essential enzymes for the in vitro growth of M...”
SCMU_YERPE / Q7CHH5 Secreted chorismate mutase; CM; *YpCM; EC 5.4.99.5 from Yersinia pestis (see paper)
Q7CHH5 chorismate mutase (EC 5.4.99.5) from Yersinia pestis (see paper)
y2828 putative chorismate mutase from Yersinia pestis KIM
Aligns to 35:104 / 186 (37.6%), covers 83.5% of PF01817, 46.7 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate. May play some role in the pathogenicity.
catalytic activity: chorismate = prephenate (RHEA:13897)
subunit: Homodimer. - Protein abundances can distinguish between naturally-occurring and laboratory strains of Yersinia pestis, the causative agent of plague
Merkley, PloS one 2017 - “...ATP synthase A chain (EC 3.6.3.14) Q7CFM3 0.6996 Periplasmic chorismate mutase I precursor (EC 5.4.99.5) Q7CHH5 0.6561 Inorganic pyrophosphatase (EC 3.6.1.1) Q8ZB98 0.1669 Maltose/maltodextrin ABC transporter, substrate binding periplasmic protein MalE Q7CLD8 0.1122 Biofilm PGA synthesis deacetylase PgaB (EC.3-) (HmsF) Q9R7V4 -0.4388 Attachment invasion locus protein...”
- Proteomic analysis of iron acquisition, metabolic and regulatory responses of Yersinia pestis to iron starvation
Pieper, BMC microbiology 2010 - “...periplasmic D-galactose-binding ABC transport protein PP 1440 33113 5.40 0.51 1.53 0.330 0.000 0.251 80 y2828 pheA putative chorismate mutase PP 630 14433 5.88 0.86 0.05 19.293 0.000 2.817 81 y2842 - putative periplasmic binding protein of iron/siderophore ABC transporter U 1096 51189 5.97 0.62 1.87...”
- A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H(37)R(v) and the secreted chorismate mutase (y2828) from Yersinia pestis
Kim, The FEBS journal 2008 (PubMed)- “...tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis Sook-Kyung Kim*, Sathyavelu K. Reddy, Bryant C. Nelson, Howard Robinson,...”
- “...of a-helical AroQ CMs termed AroQd. The CM gene (y2828) from Yersinia pestis encodes a 186 amino acid protein with an N-terminal signal peptide that directs the...”
5ckxD / P9WIC1 Non-covalent complex of dahp synthase and chorismate mutase from mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine (see paper)
Aligns to 7:70 / 79 (81.0%), covers 68.4% of PF01817, 45.3 bits
- Ligand: 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid (5ckxD)
2h9dA / Q51507 Pyruvate-bound structure of the isochorismate-pyruvate lyase from pseudomonas aerugionsa (see paper)
Aligns to 14:81 / 84 (81.0%), covers 98.7% of PF01817, 45.2 bits
- Ligand: pyruvic acid (2h9dA)
PF66_01042 chorismate mutase from Pseudomonas fuscovaginae
Aligns to 32:102 / 186 (38.2%), covers 87.3% of PF01817, 45.0 bits
- Rice-Infecting Pseudomonas Genomes Are Highly Accessorized and Harbor Multiple Putative Virulence Mechanisms to Cause Sheath Brown Rot
Quibod, PloS one 2015 - “...gene involved in locomotive function (PF66_05042) was highly expressed at all time points. Four (PF66_04809, PF66_01042, PF66_04639, and PF66_00996) out of six predicted secreted genes showed very early response during the interaction of rice and the Pfv -like strain IRRI 7007. The gene PF66_00996, which has...”
- “...genes during the early stage of infection may suggest the important functional role of PF66_04809, PF66_01042, PF66_04639, and PF66_00996 in Pfv -like pathogenicity. At a later stage (72 hpi), most of the tested genes had low level of expression. Overall, data obtained here revealed that Pfv...”
A1S_2084 chorismate mutase from Acinetobacter baumannii ATCC 17978
Aligns to 13:83 / 165 (43.0%), covers 86.1% of PF01817, 44.8 bits
CHMU_MYCTU / P9WIC1 Intracellular chorismate mutase; CM; EC 5.4.99.5 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
P9WIC1 chorismate mutase (EC 5.4.99.5) from Mycobacterium tuberculosis (see 3 papers)
Rv0948c hypothetical protein from Mycobacterium tuberculosis H37Rv
NP_215463 chorismate mutase from Mycobacterium tuberculosis H37Rv
MT0975 hypothetical protein from Mycobacterium tuberculosis CDC1551
Aligns to 33:95 / 105 (60.0%), covers 68.4% of PF01817, 44.3 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.
catalytic activity: chorismate = prephenate (RHEA:13897)
subunit: Homodimer. Interacts with AroG. - Transposon sequencing reveals metabolic pathways essential for Mycobacterium tuberculosis infection
Block, PLoS pathogens 2024 - “...mutase [ 45 ]. Two M . tuberculosis proteins have chorismate mutase activity (Rv1885c and Rv0948c), but Rv1885c is secreted while Rv0948c is cytosolic [ 47 49 ]. Only rv0948c is a M-ES gene [ 11 ], suggesting that it functions as the chorismate mutase in...”
- A secreted form of chorismate mutase (Rv1885c) in Mycobacterium bovis BCG contributes to pathogenesis by inhibiting mitochondria-mediated apoptotic cell death of macrophages
Lee, Journal of biomedical science 2023 - “...]. There are two types of genes encoding CMs in the Mtb genome: Rv1885c and Rv0948c. Of these CMs, secretory TBCM (encoded by Rv1885c) is considered to have an important role in TB pathogenesis, showing a low level of sequence homology with intracellular CM, Rv0948c [...”
- What Drives Chorismate Mutase to Top Performance? Insights from a Combined In Silico and In Vitro Study
Thorbjørnsrud, Biochemistry 2023 - “...an N-terminal His 6 tag, first 5 residues missing). Parts of the MtCM gene (Gene Rv0948c) were amplified using oligonucleotides 412-MtCM-N-V55D (5-GTTCGCTAGCGGAGGTACACGTTTGGATCATAGTCGGGAGATGAAGGTCATCGAAC) or 413-MtCM-N-T52P (5-GTTCGCTAGCGGAGGTCCGCGTTTGGTCCATAGTCGGGAGATGAAGGTCATCGAAC) together with oligonucleotides 386-LpLib-N2 (5-GGTTAAAGCTTCCGCAGCCACTAGTTATTAGTGACCGAGGCGGCCACGGCCCAAT) on template pMG248 12 to create a 163 bp PCR product. The PCR products were restriction...”
- “...statistics (Table S1) ( PDF ) Supplementary Material bi2c00635_si_001.pdf Accession Codes UniProt ID: P9WIC1 (Gene Rv0948c). PDB ID: 6YGT . Author Contributions H.V.T., L.B., and T.K. contributed equally to this work. Author Contributions U.K. conceived the study. H.V.T., P.K., and Mi.C. were additionally involved in the...”
- Uncovering Beta-Lactam Susceptibility Patterns in Clinical Isolates of Mycobacterium tuberculosis through Whole-Genome Sequencing
Olivença, Microbiology spectrum 2022 - “...and cut3 ); lipid metabolism ( mmaA4 and papA1 ); intermediary metabolism and respiration ( rv0948c and hisI ); information pathways ( hsdM and pheT ); regulatory proteins ( rv0342 and pknA ); insertion sequences and phages ( rv1128c ); conserved hypotheticals ( rv2022c, rv0791c, rv3057c...”
- “...1.52E06 (3.24E02) Low Rv0642c mmaA4 736710 T>C N165S 61 - - - 1.52E06 (3.24E02) Low Rv0948c - 1057788 T>G K59T 61 - - - 1.52E06 (3.24E02) Low Rv1606 hisI 1805948 C>T T99I 61 - - - 1.52E06 (3.24E02) Low Rv1884c rpfC 2134215 T>C H16R 61 -...”
- Potential of Mycobacterium tuberculosis chorismate mutase (Rv1885c) as a novel TLR4-mediated adjuvant for dendritic cell-based cancer immunotherapy
Jeong, Oncoimmunology 2022 - “...conversion of chorismate to prephenate. 31 There are two putative genes for CM: Rv1885c and Rv0948c. Of these, the secretory form, TBCM (encoded by Rv1885c), is assumed to play a key role in the pathogenesis of tuberculosis, 32 and it has low sequence homology among known...”
- Commonalities of Mycobacterium tuberculosis Transcriptomes in Response to Defined Persisting Macrophage Stresses
Vilchèze, Frontiers in immunology 2022 - “...of chorismate to these aromatic amino acids ( trpABCE, pheA, tyrA and the chorismate mutase Rv0948c ) were also not induced or were repressed ( Figure4 ; Table S2c ). Chorismate is also involved in the synthesis of menaquinone, folate, and siderophores. Only the folate pathway...”
- Aptamer-Based Diagnostic Systems for the Rapid Screening of TB at the Point-of-Care
Martin, Diagnostics (Basel, Switzerland) 2021 - “...binding (Ssb) protein or helix-destabilizing protein a,b Rv0054 + [ 150 ] Chorismate mutase a Rv0948c + [ 150 ] Heat shock protein HspX a Rv2031c - [ 150 ] Conserved protein a,b Rv0831c 0 [ 150 ] Possible transcriptional regulatory protein b Rv3405c + [...”
- Dissecting the antibacterial activity of oxadiazolone-core derivatives against Mycobacterium abscessus
Madani, PloS one 2020 - “...Rv3626c - uncharacterized protein - MAB_0684c 26.813 Rv0774c CF Hypothetical extracellular esterase CW/CP MAB_1053c 10.305 Rv0948c In vitro growth WCL Chorismate mutase IM/R MAB_1675 28.418 Rv2362c In vitro growth CW Possible DNA repair protein RecO IP MAB_2366 33.804 Rv1701 - Probable integrase RP MAB_2477c 55.217 Rv1393c...”
- “...protein RecO ( i . e ., Rv2362c), and MAB_1053c ( i . e ., Rv0948c) a putative chorismate mutase possibly involved in phenylalanine, tyrosine and tryptophan biosynthesis, are annotated as essential enzymes for the in vitro growth of M . tuberculosis [ 44 , 45...”
- More
- Remote Control by Inter-Enzyme Allostery: A Novel Paradigm for Regulation of the Shikimate Pathway.
Munack, Journal of molecular biology 2016 (PubMed)- GeneRIF: Here, the authors show how DAHP synthase serves as an allosteric platform for feedback regulation of both enzymes, DAHP synthase and chorismate mutase.
- Structure and function of a complex between chorismate mutase and DAHP synthase: efficiency boost for the junior partner.
Sasso, The EMBO journal 2009 - GeneRIF: The intracellular chorismate mutase of Mycobacterium tuberculosis (MtCM; Rv0948c) has poor activity and lacks prominent active-site residues. However, its catalytic efficiency increases >100-fold on addition of DAHP synthase (MtDS; Rv2178c).
- A novel noncovalent complex of chorismate mutase and DAHP synthase from Mycobacterium tuberculosis: protein purification, crystallization and X-ray diffraction analysis.
Okvist, Acta crystallographica. Section F, Structural biology and crystallization communications 2009 - GeneRIF: MtCM was crystallized alone and in complex with MtDS. The crystals diffracted to 1.6 and 2.2 A resolution, respectively.
- A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H(37)R(v) and the secreted chorismate mutase (y2828) from Yersinia pestis.
Kim, The FEBS journal 2008 (PubMed)- GeneRIF: The 2.0A X-ray structure shows that 90-MtCM is an all alpha-helical homodimer with the topology of Escherichia coli CM, and that both protomers contribute to each catalytic site.
- What Drives Chorismate Mutase to Top Performance? Insights from a Combined In Silico and In Vitro Study
Thorbjørnsrud, Biochemistry 2023 - “...and refinement statistics (Table S1) ( PDF ) Supplementary Material bi2c00635_si_001.pdf Accession Codes UniProt ID: P9WIC1 (Gene Rv0948c). PDB ID: 6YGT . Author Contributions H.V.T., L.B., and T.K. contributed equally to this work. Author Contributions U.K. conceived the study. H.V.T., P.K., and Mi.C. were additionally involved...”
- Synthetic lethality reveals mechanisms of Mycobacterium tuberculosis resistance to β-lactams
Lun, mBio 2014 - “...0.54, respectively (see TableS2 in the supplemental material). In addition, the chorismate mutase gene ( MT0975 ) also demonstrated GCSL with penicillin ( TableS2 ). Interestingly, some strains exhibited hyper-resistance to penicillin; these included the MT1661 (FC = 2.54), MT3826 (FC = 2.40), and MT3310 (FC...”
- “...contained mutations in the conserved outer membrane peptidase-like protein ( MT0335 ), chorismate mutase ( MT0975 ), putative acyl-transferase ( MT1616 ), putative carboxylesterase ( MT2282 ), and l , d -transpeptidase ( MT2954 ) genes. MICs of imipenem and clarithromycin were determined using the microplate...”
AKJ12_RS15475 chorismate mutase from Xanthomonas arboricola pv. juglandis
Aligns to 35:105 / 189 (37.6%), covers 87.3% of PF01817, 44.2 bits
- A Secreted Chorismate Mutase from Xanthomonas arboricola pv. juglandis Attenuates Virulence and Walnut Blight Symptoms
Assis, International journal of molecular sciences 2021 - “...In this work, we describe the use of a secreted CM knockout mutant of Xaj417 (AKJ12_RS15475 XajCM) to define its role in blight disease development. Proteomic analysis by LC/MS-MS of walnut hulls inoculated with Xaj WT or XajCM revealed that all differentially abundant proteins found in...”
- “...4. Materials and Methods 4.1. Generation of the XajCM Mutant To disrupt monofunctional chorismate mutase (AKJ12_RS15475), a mutagenesis cassette was synthesized (GenScript, NJ USA) by inserting a kanamycin resistance gene (Tn903 aminoglycoside transferase) within XajCMs lysine residue at the coding region amino acid position 163, a...”
PD0426 chorismate mutase from Xylella fastidiosa Temecula1
Aligns to 37:107 / 191 (37.2%), covers 87.3% of PF01817, 44.2 bits
- The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa
Gouran, Scientific reports 2016 - “...pyrE (PD0122), purA (PD1627), purE (PD2036), amino acids argH (PD0295), proB (PD0296), proA (PD0297), pheA (PD0426), aroK (PD0582), asd (PD0608), aspH (PD0777), trpDE (PD0876 and PD0877), serC (PD1358), leuB (PD1397), cofactor recycling cysG (PD1840), folP (PD0068), ptr1 (PD0677), nadA (PD0869), grx (PD1409), ribF (PD1438), folB (PD1642),...”
- The Type II Secreted Lipase/Esterase LesA is a Key Virulence Factor Required for Xylella fastidiosa Pathogenesis in Grapevines
Nascimento, Scientific reports 2016 - “...0.728400 7.23% 5 Q87CV6 Serine protease PD0950 OM 96.0 0.914469 7.26% 4 Q87E92 Chorismate mutase PD0426 P 21.4 0.115485 34.0% 6 Q87CV2 Putat. Unchar. Prot. PD0956 U 37.2 0.890737 21.6% 6 Q87BZ7 Putat. unchar. protein PD1299 U 55.7 0.898888 11.1% 6 Q87D31 VirK protein PD0855 U...”
ABAYE1477 monofunctional chorismate mutase precursor (CM-F) from Acinetobacter baumannii AYE
Aligns to 33:103 / 185 (38.4%), covers 86.1% of PF01817, 44.1 bits
ACPL_887 chorismate mutase from Actinoplanes sp. SE50/110
Aligns to 22:84 / 93 (67.7%), covers 65.8% of PF01817, 43.0 bits
PXO_RS21360 chorismate mutase from Xanthomonas oryzae pv. oryzae PXO99A
Aligns to 35:105 / 189 (37.6%), covers 87.3% of PF01817, 42.8 bits
- Melatonin Treatment Inhibits the Growth of Xanthomonas oryzae pv. oryzae
Chen, Frontiers in microbiology 2018 - “...PXO_RS27075 +1.86 Fumarylacetoacetate hydrolase domain protein 31 PXO_RS08315 -2.97 Bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase 32 PXO_RS21360 -2.23 Chorismate mutase 33 PXO_RS20590 -4.82 Glycerophosphodiester phosphodiesterase 34 PXO_RS00340 -2.69 Aldolase 35 PXO_RS00350 -2.69 Aldolase 36 PXO_RS23355 -1.69 Cellulase 37 PXO_RS05615 -3.88 Xylanase 38 PXO_RS01665 -2.32 Xylose isomerase 39...”
PA14_68480 putative periplasmic monofunctional chorismate mutase from Pseudomonas aeruginosa UCBPP-PA14
Aligns to 31:101 / 185 (38.4%), covers 89.9% of PF01817, 42.3 bits
- Dissecting the machinery that introduces disulfide bonds in Pseudomonas aeruginosa
Arts, mBio 2013 - “...Q02QW4 Cyclohexadienyl dehydratase PheC (PA14_19140) 2 d P 0.33 Q02EI0 Putative periplasmic monofunctional chorismate mutase (PA14_68480) 2 d P 0.00 Q02PI4 Putative soluble lytic transglycosylase Slt (PA14_25000) 2 P 0.00 Q02PL1 Putative d -alanyl- d -alanine-carboxypeptidase DacB (PA14_24690) 2 P 0.00 Q02GT9 Putative binding protein component...”
CHMU_PSEAE / Q9HU05 Monofunctional chorismate mutase; EC 5.4.99.5 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
PA5184 chorismate mutase from Pseudomonas aeruginosa PAO1
Aligns to 31:101 / 185 (38.4%), covers 89.9% of PF01817, 42.3 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate. The joint presence of this enzyme together with cyclohexadienyl dehydratase and aromatic aminotransferase in the periplasmic compartment comprises a complete three-step chorismate to phenylalanine pathway and accounts for the so called hidden overflow pathway to phenylalanine in P.aeruginosa, in which two possible routes for it exists, namely either via phenylpyruvate or L-arogenate.
catalytic activity: chorismate = prephenate (RHEA:13897)
subunit: Homodimer. - An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa
Marden, Proceedings of the National Academy of Sciences of the United States of America 2013 - “...located in the intergenic region between genes PA5183 and PA5184 (SI Appendix, Fig. S1A). The predicted ORF encodes a 71-aa protein bearing 31% identity and 53%...”
- Structural rearrangement in an RsmA/CsrA ortholog of Pseudomonas aeruginosa creates a dimeric RNA-binding protein, RsmN
Morris, Structure (London, England : 1993) 2013 - “...the RsmA regulatory pathway revealed that the 513bp intergenic region between open reading framesPA5183 and PA5184 contained an unannotated open reading frame encoding a 7.8kDa protein ( Figure1 A). We termed this gene rsmN due to its novel sequence and to highlight its more distant relatedness...”
- Regulatory and metabolic networks for the adaptation of Pseudomonas aeruginosa biofilms to urinary tract-like conditions
Tielen, PloS one 2013 - “...sequential steps via the phenate dehydrogenase encoded by PA3164 and the chorismate dehydrogenase encoded by PA5184. Again, both genes were found to be induced. Subsequently, chorismate is converted by the anthranilate synthase PhnA to anthranilate, the direct precursor of the PQS [84] . Corresponding gene phnA...”
- Global position analysis of the Pseudomonas aeruginosa quorum-sensing transcription factor LasR
Gilbert, Molecular microbiology 2009 - “...Greenberg, 2007 ). Eight promoter fragments ( rsaL , PA0026, PA2069, PA2305, PA2763, PA4117, PA4305/6, PA5184), and fragments internal to the lasB , PA0996, PA1914, and PA4677 genes were selected for analysis by qPCR. The latter served as background controls. Primers were designed such that the...”
- “...ATP-binding component of ABC phosphonate transporter PA3385* ( amrZ ) NDE Alginate and motility regulator PA5184 6.6 NDE N CAATCAGAAATGTCCC j (114 to 99) - Hypothetical protein a LasR-ChIP enriched regions are indicated by the PA number of the associated gene or operon. The respective gene...”
- The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa
Calhoun, Genome biology 2001 - “...the single-letter amino-acid notation), which corresponds to a sequence beginning at residue 24 of gene PA5184 of the completed P. aeruginosa genome [ 19 ]. Table 4 Purification of *AroQ from Pseudomonas aeruginosa Purification Total protein Specific Purification Yield (mg) activity * (fold) (%) Shock fluid...”
B2JYH9 chorismate mutase (EC 5.4.99.5) from Paraburkholderia phymatum (see paper)
Aligns to 26:95 / 182 (38.5%), covers 87.3% of PF01817, 42.1 bits
8pnhA Chorismate mutase
Aligns to 239:307 / 386 (17.9%), covers 81.0% of PF01817, 40.5 bits
- Ligands: 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid; 3-phenylpyruvic acid (8pnhA)
WP_003484013, XAC_RS18440 chorismate mutase from Xanthomonas citri pv. citri
XAC3647 chorismate mutase from Xanthomonas axonopodis pv. citri str. 306
Aligns to 35:105 / 189 (37.6%), covers 87.3% of PF01817, 40.5 bits
Q74NC4 prephenate dehydratase (EC 4.2.1.51) from Nanoarchaeum equitans (see paper)
NEQ192 NEQ192 from Nanoarchaeum equitans Kin4-M
Aligns to 244:322 / 591 (13.4%), covers 98.7% of PF01817, 40.3 bits
E2P69_RS04815 chorismate mutase from Xanthomonas perforans
Aligns to 35:105 / 189 (37.6%), covers 87.3% of PF01817, 40.3 bits
XCV3765 Chorismate mutase precursor from Xanthomonas campestris pv. vesicatoria str. 85-10
Aligns to 35:105 / 189 (37.6%), covers 87.3% of PF01817, 40.3 bits
CHMU_MYCS2 / A0R3N5 Intracellular chorismate mutase; CM; EC 5.4.99.5 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
Aligns to 31:96 / 104 (63.5%), covers 69.6% of PF01817, 39.3 bits
- function: Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.
catalytic activity: chorismate = prephenate (RHEA:13897)
subunit: Homodimer (By similarity). Probably interacts with AroG (MSMEG_4244) (PubMed:17965159).
M271_28280 chorismate mutase from Streptomyces rapamycinicus NRRL 5491
Aligns to 18:84 / 89 (75.3%), covers 65.8% of PF01817, 37.6 bits
TK0261 predicted chorismate mutase, N-truncation from Thermococcus kodakaraensis KOD1
Aligns to 1:50 / 58 (86.2%), covers 75.9% of PF01817, 36.9 bits
- The TK0271 Protein Activates Transcription of Aromatic Amino Acid Biosynthesis Genes in the Hyperthermophilic Archaeon Thermococcus kodakarensis
Yamamoto, mBio 2019 - “...of two complementary oligonucleotides using a 5-biotinylated primer. For the genes TK0252 ( trp ), TK0261 ( tyr-phe ), TK0271 ( aro ), TK0705, and TK1899, an 200-bp promoter region containing BRE-TATA sequences together with a 10- to 15-bp portion of the ORF was amplified with...”
- “...TK0271 and a region spanning 192bp upstream and 251bp downstream of the initiation codon of TK0261 were amplified using the primer sets PTK0271_F/PTK0271_R_2 and PTK0261_F/PTK0261_R_2, respectively. Amplified fragments were ligated in the HincII site of pUC18. The resulting plasmid was used as a template to amplify...”
8pnjA Chorismate mutase
Aligns to 2:73 / 390 (18.5%), covers 88.6% of PF01817, 36.6 bits
- Ligand: 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid (8pnjA)
Q3LUE0 chorismate mutase (EC 5.4.99.5) from Heterodera schachtii (see paper)
Aligns to 101:170 / 275 (25.5%), covers 87.3% of PF01817, 36.3 bits
5hudE / Q8NS29 Non-covalent complex of and dahp synthase and chorismate mutase from corynebacterium glutamicum with bound transition state analog (see paper)
Aligns to 12:76 / 84 (77.4%), covers 65.8% of PF01817, 35.7 bits
- Ligand: 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid (5hudE)
B6C761 chorismate mutase (EC 5.4.99.5) from Globodera rostochiensis (see paper)
Aligns to 110:179 / 286 (24.5%), covers 87.3% of PF01817, 35.3 bits
cg0975 hypothetical protein from Corynebacterium glutamicum ATCC 13032
CGS9114_RS13620, NCgl0819 chorismate mutase from Corynebacterium glutamicum S9114
Aligns to 29:93 / 101 (64.4%), covers 65.8% of PF01817, 35.0 bits
- Can Genome Sequencing Coupled to Flux Balance Analyses Offer Precision Guidance for Industrial Strain Development? The Lessons from Carbon Trafficking in Corynebacterium glutamicum ATCC 21573
Kurpejović, Omics : a journal of integrative biology 2023 (PubMed)- “...export was probabilistically impossible, which could have contributed to the l-Tyr accumulation. Interestingly, aroG and cg0975, which have received previous attention for aromatic amino acid overproduction, were not mutated. From the branch point molecule, prephenate, the change in the promoter region of pheA could be an...”
- De novo tryptophanase-based indole production by metabolically engineered Corynebacterium glutamicum
Mindt, Applied microbiology and biotechnology 2023 - “...) (Schfer et al. 1994 ) pK19- csm pK19 mobsacB derivative for deletion of csm (cg0975) (Li et al. 2009 ) pK19 yggB pK19 mobsacB derivative for deletion of yggB (cg1434) (Prez-Garca et al. 2019 ) pK19- trpL:: P ilvC -M1 trpE S38R Cg pK19 mobsacB...”
- Fermentative Indole Production via Bacterial Tryptophan Synthase Alpha Subunit and Plant Indole-3-Glycerol Phosphate Lyase Enzymes
Ferrer, Journal of agricultural and food chemistry 2022 - “...trpBA (cg3363-cg3364) thiswork pK19- csm pK19 mobsacB with a construct for the deletion of csm (cg0975) ( 23 ) pK19- yggB pK19 mobsacB with a construct for the deletion of yggB (cg1434) ( 24 ) pK19- trpL ::P ilvC- M1 trpE Cg S38R pK19 mobsacB with...”
- CRISPR-Driven Genome Engineering for Chorismate- and Anthranilate-Accumulating Corynebacterium Cell Factories
Kim, Journal of microbiology and biotechnology 2023 - “...be metabolized by other CHR metabolic pathways ( Fig. 2B ). It is suspected that NCgl0819 is involved in the conversion of CHR to prephenate, PabAB (NCgl0955) in the synthesis of para-aminobenzoate, and EntC (NCgl1243) in the conversion of CHR to isochorismate. To induce the overaccumulation...”
- “...trpE & trpG This study Inha341 Inha304 aroK :: aroK trpE & trpG & trpD NCgl0819 pabAB entC This study Inha342 Inha353 trpE & trpG This study Inha343 Inha354 trpE & trpG This study Inha344 Inha343 iolR :: Ptuf_ ppgK & glk This study Inha345 Inha344...”
- Improvement in L-ornithine production from mannitol via transcriptome-guided genetic engineering in Corynebacterium glutamicum
Nie, Biotechnology for biofuels and bioproducts 2022 - “...MFS transporter 2.47 benK Up CGS9114_RS08620 Benzoate/H (+) symporter BenE family transporter 2.00 benE Up CGS9114_RS13620 Chorismate mutase 1.44 pheA1 Up CGS9114_RS03770 FAD-binding oxidoreductase 1.15 dadA Up CGS9114_RS03835 3-Hydroxybenzoate 6-hydroxylase 1.13 nagX Up CGS9114_RS08680 Protocatechuate 3%2C4-dioxygenase subunit alpha 1.09 pcaG Up CGS9114_RS08660 Benzoate degradation 1.07 catB...”
- Genetic and biochemical identification of the chorismate mutase from Corynebacterium glutamicum
Li, Microbiology (Reading, England) 2009 (PubMed)- “...identified in C. glutamicum. In this study, the ncgl0819 gene, which was annotated as `conserved hypothetical protein' in the C. glutamicum genome, was...”
- “...growth in minimal medium, and a mutant deleted of ncgl0819 was a Phe and Tyr auxotroph. Genetic cloning and expression of ncgl0819 in Escherichia coli resulted...”
- Corynebacterium glutamicum contains 3-deoxy-D-arabino-heptulosonate 7-phosphate synthases that display novel biochemical features
Liu, Applied and environmental microbiology 2008 - “...observed (23). A putative chorismate mutase, encoded by NCgl0819, has been proposed for C. glutamicum ATCC 13032 (11, 15). Characterization of the chorismate...”
SCO4784 hypothetical protein from Streptomyces coelicolor A3(2)
Aligns to 39:106 / 110 (61.8%), covers 65.8% of PF01817, 34.9 bits
SLUN_RS38500 chorismate mutase from Streptomyces lunaelactis
Aligns to 29:93 / 100 (65.0%), covers 64.6% of PF01817, 29.7 bits
Or search for genetic data about PF01817 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory