Family Search for PF02593 (DUF166)

PF02593 hits 4 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

MMP_RS05055 DUF166 domain-containing protein from Methanococcus maripaludis S2
Aligns to 4:214 / 218 (96.8%), covers 99.5% of PF02593, 264.3 bits

• High-throughput genetics enables identification of nutrient utilization and accessory energy metabolism genes in a model methanogen
  Day, mBio 2024
  • “...quantified by the linear Pearson's correlation, was r = 0.810.86). Fig 1 DUF166 encoded by MMP_RS05055 is important for fitness during autotrophic growth. ( A ) The Wood-Ljungdahl pathway for CO 2 fixation, highlighted in blue, uses methyl-H 4 MPT from the methanogenic pathway (left) for...”
  • “...of carbon monoxide dehydrogenase/acetyl-coenzyme synthase (CODH-ACS) subunit alpha ( cdhA, MMP_RS05100) and DUF166 encoded by MMP_RS05055. Experiments identified as autotrophic conditions were performed in defined minimal media both with and without vitamins and either H 2 or formate as the electron donor. Diagram of a metabolic...”

MMJJ_RS00080 DUF166 domain-containing protein from Methanococcus maripaludis
Aligns to 4:214 / 218 (96.8%), covers 99.1% of PF02593, 256.2 bits

• High-throughput genetics enables identification of nutrient utilization and accessory energy metabolism genes in a model methanogen
  Day, mBio 2024
  • “...genes encoding the CODH-ACS operon, DUF166, and MMP_RS05060 is conserved in strain JJ as well (MMJJ_RS00080 and MMJJ_RS00080; r = 0.960.99). Both MMP_RS05060 and MMP_RS05085 (in the CODH-ACS gene cluster) are annotated to encode the CooC domain predicted to be involved in maturation of the nickel...”

MJ0757 / Q58167 thymidylate synthase (EC 2.1.1.45) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
TMPS_METJA / Q58167 Thymidylate synthase; TS; TSase; EC 2.1.1.- from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Aligns to 20:260 / 260 (92.7%), covers 97.7% of PF02593, 255.4 bits

• function: Is able to catalyze the biosynthesis of dTMP using dUMP, tetrahydrofolate and formaldehyde in vitro, i.e. a reaction equivalent to that catalyzed by bacterial thymidylate synthases (EC 2.1.1.45). However, M.jannaschii like most methanogenic Archaea lacks folates, thus the physiological cosubstrate is unknown but is likely one of the non-methylated methanopterin biosynthetic intermediates.

MMP0986 thymidylate synthase from Methanococcus maripaludis S2
Aligns to 4:221 / 291 (74.9%), covers 99.1% of PF02593, 252.9 bits

• Synergistic epistasis enhances the co-operativity of mutualistic interspecies interactions
  Turkarslan, The ISME journal 2021
  • “...simultaneous fixation of mutations in multiple genes (e.g., DVU0799 and DVU1214 in HE3; MMP0378, MMP0705, MMP0986, and MMP1170 in HA2) suggesting that hitchhiking may be common [ 30 , 31 ]. However, given that samples were only sequenced every 250 generations, we cannot rule out the...”
• Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis
  Hendrickson, Journal of bacteriology 2004
  • “...(Mmp1067) and one of the two thymidylate synthases (Mmp0986). In addition, the functions of many iron-sulfur proteins found in the genome are not known....”
  • “...is then converted to dTMP by thymidylate synthase (ThyA; Mmp0986 and Mmp1379). dTMP is converted to dTDP by thymidylate kinase (Tmk; Mmp1034) and thence to dTTP...”

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