Family Search for PF02593 (DUF166)

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PF02593 hits 2 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

MJ0757 / Q58167 thymidylate synthase (EC 2.1.1.45) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
TMPS_METJA / Q58167 Thymidylate synthase; TS; TSase; EC 2.1.1.- from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Aligns to 20:260 / 260 (92.7%), covers 98.6% of PF02593, 259.2 bits

• function: Is able to catalyze the biosynthesis of dTMP using dUMP, tetrahydrofolate and formaldehyde in vitro, i.e. a reaction equivalent to that catalyzed by bacterial thymidylate synthases (EC 2.1.1.45). However, M.jannaschii like most methanogenic Archaea lacks folates, thus the physiological cosubstrate is unknown but is likely one of the non-methylated methanopterin biosynthetic intermediates.

MMP0986 thymidylate synthase from Methanococcus maripaludis S2
Aligns to 4:221 / 291 (74.9%), covers 99.5% of PF02593, 248.1 bits

• Synergistic epistasis enhances the co-operativity of mutualistic interspecies interactions
  Turkarslan, The ISME journal 2021
  • “...simultaneous fixation of mutations in multiple genes (e.g., DVU0799 and DVU1214 in HE3; MMP0378, MMP0705, MMP0986, and MMP1170 in HA2) suggesting that hitchhiking may be common [ 30 , 31 ]. However, given that samples were only sequenced every 250 generations, we cannot rule out the...”
• Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis
  Hendrickson, Journal of bacteriology 2004
  • “...(Mmp1067) and one of the two thymidylate synthases (Mmp0986). In addition, the functions of many iron-sulfur proteins found in the genome are not known....”
  • “...is then converted to dTMP by thymidylate synthase (ThyA; Mmp0986 and Mmp1379). dTMP is converted to dTDP by thymidylate kinase (Tmk; Mmp1034) and thence to dTTP...”

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