Family Search for PF04028 (DUF374)
PF04028 hits 16 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
CJJ81176_0482 hypothetical protein from Campylobacter jejuni subsp. jejuni 81-176
Aligns to 65:133 / 216 (31.9%), covers 100.0% of PF04028, 97.1 bits
- Identification of a broad family of lipid A late acyltransferases with non-canonical substrate specificity
Rubin, Molecular microbiology 2014 - “...of LpxJ instead of LpxM. In two closely related Epsilonproteobacteria, we identified Campylobacter jejuni protein Cjj81176_0482 (LpxJ Cj ) and Wolinella succinogenes protein Ws1775 (LpxJ Ws ) as homologs of LpxJ Hp and members of the DUF374 family. The C. jejuni and W. succinogenes proteins were...”
- “...of H. pylori J99 ( jhp0255 , primers 7 and 8), C. jejuni 81176 ( cjj81176_0482 , primers 9 and 10), and W. succinogenes ( ws1775 , primers 11 and 12) were cloned into vector pET21a (Novagen) behind the T7 lac promoter. The PCR product from...”
- Colonize, evade, flourish: how glyco-conjugates promote virulence of Helicobacter pylori
Rubin, Gut microbes 2013 - “...closely related species including Campylobacter jejuni (cjj81176_0482) and Wolinella succinogenes (ws1775). Hp0270 and homologs restore production of flagella...”
WS1775 LIPOPROTEIN from Wolinella succinogenes DSM 1740
Aligns to 55:123 / 200 (34.5%), covers 95.7% of PF04028, 95.9 bits
- Identification of a broad family of lipid A late acyltransferases with non-canonical substrate specificity
Rubin, Molecular microbiology 2014 - “...related Epsilonproteobacteria, we identified Campylobacter jejuni protein Cjj81176_0482 (LpxJ Cj ) and Wolinella succinogenes protein Ws1775 (LpxJ Ws ) as homologs of LpxJ Hp and members of the DUF374 family. The C. jejuni and W. succinogenes proteins were expressed individually in strain BN2 and radiolabeled lipid...”
- “...8), C. jejuni 81176 ( cjj81176_0482 , primers 9 and 10), and W. succinogenes ( ws1775 , primers 11 and 12) were cloned into vector pET21a (Novagen) behind the T7 lac promoter. The PCR product from jhp0255 , amplification was digested with NdeI and BamHI, and...”
- Colonize, evade, flourish: how glyco-conjugates promote virulence of Helicobacter pylori
Rubin, Gut microbes 2013 - “...including Campylobacter jejuni (cjj81176_0482) and Wolinella succinogenes (ws1775). Hp0270 and homologs restore production of flagella is blocked. Adding to the...”
LIC12382 conserved hypothetical protein from Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
Aligns to 76:144 / 238 (29.0%), covers 100.0% of PF04028, 93.0 bits
- Analysis of LexA binding sites and transcriptomics in response to genotoxic stress in Leptospira interrogans
Schons-Fonseca, Nucleic acids research 2016 - “...synthesis and modification of the membrane and peptideoglycan (LIC13392 and wzyC ), central metabolism (LIC12298, LIC12382, LIC10987, LIC12595, fbp , LIC12975 and risA ), motility ( fliF ), biopolymer transport (LIC11622), tRNA modification ( gidA ) and hypothetical proteins (LIC12260, LIC10986 and LIC11622). Figure 5. (...”
- “...Supplementary Table S5). Seven of the SOS boxes were present inside a coding sequence, namely LIC12382, LIC10987, mutY, fbp , LIC12975, wzyC and gidA (Figure 5A and Supplementary Figure S3). Apart from gidA and fbp , these genes were up regulated after UV-C irradiation, suggesting LexA1...”
CCNA_00306 hypothetical protein from Caulobacter crescentus NA1000
Aligns to 71:146 / 237 (32.1%), covers 94.2% of PF04028, 92.2 bits
lpxJ / O25048 acyl-Kdo2-lipid IVA acyltransferase (EC 2.3.1.243) from Helicobacter pylori (strain ATCC 700392 / 26695) (see 3 papers)
HP0270 hypothetical protein from Helicobacter pylori 26695
Aligns to 7:75 / 162 (42.6%), covers 95.7% of PF04028, 91.4 bits
- Genomic diversity of Helicobacter pylori populations from different regions of the human stomach
Wilkinson, Gut microbes 2022 - “...of conservation that might be exploited in vaccine development. For example, genetic interruption of the HP0270 gene in H. pylori , a homolog of the LpxM protein involved in lipid A biosynthesis, has been shown to be lethal. 61 Our data suggest that HP0270 is conserved...”
- Helicobacter pylori: expect the unexpected
Suarez, Molecular microbiology 2014 - “...domains, the authors cleverly identified the locus jhp0255 of H. pylori strain J99 (similar to HP0270 in H. pylori strain 26695) as an ORF encoding a product that shares homology only within the catalytic domain of E. coli LpxM. This putative enzyme, which was renamed LpxJ...”
- Colonize, evade, flourish: how glyco-conjugates promote virulence of Helicobacter pylori
Rubin, Gut microbes 2013 - “...its E. coli homolog (Fig. 3). Genetic interruption of hp0270 is lethal, suggesting that in H. pylori, MsbA requires hexa-acylated lipid A as substrate (Rubin...”
- “...functional equivalent in H. pylori, encoded by hp0270, and homologs in closely related species including Campylobacter jejuni (cjj81176_0482) and Wolinella...”
- Growth phase-dependent response of Helicobacter pylori to iron starvation
Merrell, Infection and immunity 2003 - “...0.4 0.5 0.3 0.4 0.4 0.5 Hypothetical HP0248 HP0270 HP0466 HP0554 HP0659 HP0783 #HP0838 HP0863 HP1124 HP1127 HP1233 JHP0233 JHP0255 JHP0418 JHP0501 JHP0604...”
- Acid-adaptive genes of Helicobacter pylori
Wen, Infection and immunity 2003 - “...HP0022 HP0162 HP0234 HP0552 HP0707 HP0926 HP0946 HP0161 HP0270 HP0369 HP0582 HP0603 HP0838 HP0863 HP0864 HP1029 HP1106 HP1124 HP1217 HP1334 HP1377 HP1502 HP1546...”
- Acid-induced gene expression in Helicobacter pylori: study in genomic scale by microarray
Ang, Infection and immunity 2001 - “...HP0595 HP0156 HP0746 HP1037 HP1162 HP1163 HP1211 HP0270 HP0880 HP1214 HP0222 HP0983 HP0232 Hypothetical protein Conserved hypothetical ATP binding protein...”
Q9ZMG5 acyl-Kdo2-lipid IVA acyltransferase (EC 2.3.1.243) from Helicobacter pylori (see paper)
jhp0255 putative from Helicobacter pylori J99
Aligns to 62:130 / 217 (31.8%), covers 95.7% of PF04028, 90.6 bits
- Identification of a broad family of lipid A late acyltransferases with non-canonical substrate specificity
Rubin, Molecular microbiology 2014 - “...in the pathway have been identified in this organism. Here, we identify the missing acyltransferase, Jhp0255, which transfers a secondary acyl chain to the 3-linked primary acyl chain of lipid A, an activity similar to that of E. coli LpxM. This enzyme, reannotated as LpxJ due...”
- “...equivalent of E. coli LpxM that is not identifiable based on sequence homology. We identified jhp0255 as a strong candidate to encode the missing late acyltransferase by consulting the Pfam database, an online tool that groups proteins by predicted functional domains ( Punta et al. ,...”
- Helicobacter pylori: expect the unexpected
Suarez, Molecular microbiology 2014 - “...Pfam database, which segregates proteins by predicted functional domains, the authors cleverly identified the locus jhp0255 of H. pylori strain J99 (similar to HP0270 in H. pylori strain 26695) as an ORF encoding a product that shares homology only within the catalytic domain of E. coli...”
- Growth phase-dependent response of Helicobacter pylori to iron starvation
Merrell, Infection and immunity 2003 - “...HP0783 #HP0838 HP0863 HP1124 HP1127 HP1233 JHP0233 JHP0255 JHP0418 JHP0501 JHP0604 JHP0720 JHP0776 JHP0797 JHP1053 JHP1056 JHP1154 Conserved hypothetical...”
HPYLSS1_00259 lysophospholipid acyltransferase family protein from Helicobacter pylori SS1
Aligns to 62:130 / 217 (31.8%), covers 95.7% of PF04028, 90.5 bits
RPA0062 conserved unknown protein from Rhodopseudomonas palustris CGA009
Aligns to 63:131 / 224 (30.8%), covers 97.1% of PF04028, 87.2 bits
A1C_00645 hypothetical protein from Rickettsia akari str. Hartford
Aligns to 70:138 / 218 (31.7%), covers 92.8% of PF04028, 79.5 bits
RT0047 conserved hypothetical protein from Rickettsia typhi str. wilmington
Aligns to 70:138 / 218 (31.7%), covers 95.7% of PF04028, 77.9 bits
RBEMOGI_1439 lysophospholipid acyltransferase family protein from Rickettsia bellii str. RML Mogi
Aligns to 70:138 / 218 (31.7%), covers 91.3% of PF04028, 77.6 bits
A1G_00705 hypothetical protein from Rickettsia rickettsii str. 'Sheila Smith'
Aligns to 70:138 / 218 (31.7%), covers 92.8% of PF04028, 76.9 bits
- Rickettsia Lipid A Biosynthesis Utilizes the Late Acyltransferase LpxJ for Secondary Fatty Acid Addition
Guillotte, Journal of bacteriology 2018 - “...) and have selected putative homologs from R. typhi (RT0047) (Fig. S2) and R. rickettsii (A1G_00705), here termed LpxJ Rt and LpxJ Rr , respectively, for molecular characterization. TABLE 1 Conservation between rickettsial LpxJ homologs Rickettsia species (strain) Locus tag Homology a E value % identity...”
- “...RT0047 100 100 2E161 R. prowazekii (Breinl) H375_5410 98 99 8E159 R. rickettsii (Sheila Smith) A1G_00705 88 92 2E140 R. felis (LSU) JS55_00590 89 93 1E142 R. akari (Hartford) A1C_00645 85 91 1E136 R. belli (RML Mogi) RBEMOGI_1439 79 90 2E129 a BLAST analysis was performed...”
JS55_00590 lysophospholipid acyltransferase family protein from Rickettsia felis str. LSU
Aligns to 70:138 / 218 (31.7%), covers 92.8% of PF04028, 76.8 bits
H375_5410 lysophospholipid acyltransferase family protein from Rickettsia prowazekii str. Breinl
Aligns to 70:138 / 218 (31.7%), covers 95.7% of PF04028, 76.3 bits
lpxJ / Q2YMG0 Kdo2-lipid IVA acyltransferase (EC 2.3.1.241) from Brucella abortus (strain 2308) (see paper)
Aligns to 58:131 / 220 (33.6%), covers 92.8% of PF04028, 70.6 bits
BMEII1030 putative lipoprotein from Brucella melitensis 16M
Aligns to 79:155 / 247 (31.2%), covers 95.7% of PF04028, 64.0 bits
Or search for genetic data about PF04028 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory