Family Search for PF04502 (Saf4_Yju2)

April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.

PF04502 hits 21 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

YJU2_DANRE / A8WHR3 Splicing factor YJU2 from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
Aligns to 9:341 / 345 (96.5%), covers 100.0% of PF04502, 397.7 bits

• function: Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA. Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates (By similarity). May protect cells from TP53-dependent apoptosis upon dsDNA break damage through association with PRP19-CD5L complex (PubMed:22952453).
  subunit: Component of the spliceosome. Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron.
  disruption phenotype: Morpholino knockdown of the protein causes small head and a curled tail by day 2. Knockdown causes increased sensitivity to IR-induced apoptosis (PubMed:22952453). In double morpholino knockdown of TP53 and YJU2, TP53 deficiency rescues animals from developmental neurodegeneration observed on YJU2 mutants and radiosensitivity (PubMed:22952453).

YJU2_HUMAN / Q9BW85 Splicing factor YJU2; Coiled-coil domain-containing protein 94 from Homo sapiens (Human) (see 2 papers)
Aligns to 9:318 / 323 (96.0%), covers 100.0% of PF04502, 388.7 bits

• function: Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA (PubMed:29301961). Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'- intermediates (By similarity). May protect cells from TP53-dependent apoptosis upon dsDNA break damage through association with PRP19-CD5L complex (PubMed:22952453).
  subunit: Component of the spliceosome (PubMed:29301961). Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron (By similarity). Identified in the spliceosome C complex; in the complex interacts directly with PRP16 (PubMed:29301961).

YJU2B_HUMAN / P13994 Probable splicing factor YJU2B; Coiled-coil domain-containing protein 130 from Homo sapiens (Human) (see paper)
Aligns to 9:395 / 396 (97.7%), covers 100.0% of PF04502, 320.1 bits

• function: May be involved in mRNA splicing.

YJU2_SCHPO / Q9P7C5 Splicing factor YJU2; Complexed with cdc5 protein 16; Pre-mRNA-splicing factor cwf16 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
Aligns to 9:269 / 270 (96.7%), covers 64.6% of PF04502, 223.6 bits

• function: Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA. Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates.
  subunit: Component of the spliceosome (PubMed:11884590). Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron (By similarity). Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub- complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5, cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27, cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16, cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26, cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10, prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1, smd1, smd3, smf1, smg1 and syf2 (PubMed:11884590).

CWC16_SCHPO / O60141 Protein saf4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
Aligns to 8:293 / 299 (95.7%), covers 65.5% of PF04502, 174.0 bits

• function: May be involved in mRNA splicing.

YJU2_YEAST / P28320 Splicing factor YJU2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 9 papers)
Aligns to 9:273 / 278 (95.3%), covers 66.7% of PF04502, 158.2 bits

• function: Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA (PubMed:19935684, PubMed:27445308, PubMed:27459055, PubMed:29146871). Plays a role (via N-terminus) in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'- intermediates (PubMed:23438600, PubMed:27459055). Further stabilizes spliceosome conformation for 3'-splice site docking (via C-terminus) promoting exon ligation (PubMed:29146871).
  subunit: Component of the spliceosome. Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron (PubMed:19935684, PubMed:27445308, PubMed:27459055, PubMed:29146871). Interacts (via C-terminus) with CLF1 (PubMed:23438600). Interacts (via N-terminus) with SYF1 (PubMed:23438600). Interacts with U2 snRNA; this interaction is direct (PubMed:23438600). Identified in the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2 (PubMed:11884590).

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