Family Search for PF05304 (DUF728)

PF05304 hits 1 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

VSR_TRVPP / Q77JX3 Suppressor of RNA silencing; 16kD cysteine rich protein; P16 from Tobacco rattle virus (isolate PpK20) (TRV) (see 2 papers)
NP_620672 16 kDa protein from Tobacco rattle virus
Aligns to 2:140 / 141 (98.6%), covers 100.0% of PF05304, 328.4 bits

• function: Weak suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that performs sequence-specific inhibition of viral mRNAs expression. This could be used by the virus to infect efficiently the host the meristem cells.
• Immunocapture of dsRNA-bound proteins provides insight into Tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector
  Incarbone, The Plant cell 2021
  • “...VRCs. Detection of TRV coat protein (CP, Uniprot Q88897) and 16k suppressor of silencing (Uniprot Q77JX3) in B2:GFP IPs ( Figure2 ) also suggests that these viral proteins, not known to participate in replication, associate directly or indirectly to dsRNA. Although unlikely, we cannot at this...”
  • “...RP27a : AT1G23410; RUP1 : AT3G45570; TRV RdRP: Q9J942; TRV CP: Q88897; and TRV 16k: Q77JX3. Supplemental data The following materials are available in the online version of this article. Supplemental Figure S1 . Validation of B2 VSR activity and characterization of B2 Arabidopsis lines. Supplemental...”
• Tobacco rattle virus 16K silencing suppressor binds ARGONAUTE 4 and inhibits formation of RNA silencing complexes.
  Fernández-Calvino, The Journal of general virology 2016 (PubMed)
  • GeneRIF: 16K failed to bind small RNAs, although it interacted with Nicotiana benthamiana ARGONAUTE 4, as revealed by bimolecular fluorescence complementation and immunoprecipitation assays.
• Functional characterization and subcellular localization of the 16K cysteine-rich suppressor of gene silencing protein of tobacco rattle virus.
  Ghazala, The Journal of general virology 2008 (PubMed)
  • GeneRIF: In this study, the authors show that 16K suppresses RNA silencing in green fluorescent protein (GFP)-transgenic Nicotiana benthamiana plants using an Agrobacterium-mediated transient assay.

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