Family Search for PF06417 (DUF1077)
PF06417 hits 11 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
EMC4_HUMAN / Q5J8M3 ER membrane protein complex subunit 4; Cell proliferation-inducing gene 17 protein; Transmembrane protein 85 from Homo sapiens (Human) (see 7 papers)
NP_057538 ER membrane protein complex subunit 4 isoform a from Homo sapiens
Aligns to 56:170 / 183 (62.8%), covers 97.5% of PF06417, 157.4 bits
- function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242231, PubMed:29809151, PubMed:30415835, PubMed:32439656, PubMed:32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:29242231, PubMed:29809151, PubMed:30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:29809151, PubMed:30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29242231, PubMed:29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N- terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).
subunit: Component of the ER membrane protein complex (EMC). - Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.
Bagchi, Nature communications 2020 - GeneRIF: ER membrane protein complex subunit 4 (EMC4) and EMC7 support SV40 infection by promoting from the late endosome (LE) to the endoplasmic reticulum (ER) targeting of the virus. They do this by engaging LE-associated Rab7, to stabilize contact between the LE and ER. These EMC subunits also bind to the ER-resident fusion machinery component syntaxin18, which is required for SV40-arrival to the ER.
- Transmembrane protein 85 from both human (TMEM85) and yeast (YGL231c) inhibit hydrogen peroxide mediated cell death in yeast.
Ring, FEBS letters 2008 (PubMed)- GeneRIF: TMEM85 is a complex gene that encodes a novel conserved anti-apoptotic protein.
- Biochemical and cellular analysis of Ogden syndrome reveals downstream Nt-acetylation defects.
Myklebust, Human molecular genetics 2015 - “...RER1 (RER1) NatA M SGFLE NI O95807 Transmembrane protein 50A (TM50A) NatA M TAQGG NI Q5J8M3 Transmembrane protein 85 (TMM85) NatA M TKAGS NI Q96AG4 Leucine-rich repeat-containing protein 59 (LRC59) NatA M TKVAE NI NI Q13206 Probable ATP-dependent RNA helicase DDX10 (DDX10) NatA M TMDAL NI...”
- PredictProtein--an open resource for online prediction of protein structural and functional features.
Yachdav, Nucleic acids research 2014 - “...(UniProt AC E5A5U3). ( B ) ER membrane protein complex subunit 4 (EMC4, UniProt AC Q5J8M3). The protein sequence is represented by a scale on top of the predicted features. Features presented include proteinprotein binding sites (ISIS2), disulfide bonds (DISULFIND), structural features such as secondary structure...”
- “...simple example, the human endoplasmatic reticulum (ER) membrane protein complex subunit 4 (EMC4, UniProt AC Q5J8M3; Figure 1BE ). EMC4 is a small alpha-helical transmembrane protein with 183 residues. It is relatively well annotated, localizes to the membrane of the ER and is implicated in apoptosis...”
D4A0W1 ER membrane protein complex subunit 4 from Rattus norvegicus
Q9CZX9 ER membrane protein complex subunit 4 from Mus musculus
Aligns to 56:170 / 183 (62.8%), covers 97.5% of PF06417, 157.4 bits
XP_001657467 ER membrane protein complex subunit 4 isoform X1 from Aedes aegypti
Aligns to 46:159 / 173 (65.9%), covers 97.5% of PF06417, 153.3 bits
YQ13_SCHPO / O94520 ER membrane protein complex subunit 4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
Aligns to 62:178 / 193 (60.6%), covers 96.6% of PF06417, 153.3 bits
EMC4_YEAST / P53073 ER membrane protein complex subunit 4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
NP_011283 chaperone EMC4 from Saccharomyces cerevisiae S288C
YGL231C Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum from Saccharomyces cerevisiae
Aligns to 60:178 / 190 (62.6%), covers 97.5% of PF06417, 145.5 bits
- function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29809151). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:29809151). Involved in the cotranslational insertion of multi- pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N- exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins (By similarity).
subunit: Component of the ER membrane protein complex (EMC), which is composed of EMC1, EMC2, EMC3, EMC4, EMC5 and EMC6. - Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients
Miller-Vedam, eLife 2020 - “...Saccharomyces cerevisiae ) yEMC3 Uniprot Uniprot: P36039 Gene ( Saccharomyces cerevisiae ) yEMC4 Uniprot Uniprot: P53073 Gene ( Saccharomyces cerevisiae ) yEMC5 Uniprot Uniprot: P40540 Gene ( Saccharomyces cerevisiae ) yEMC6 Uniprot Uniprot: Q12431 Gene ( Saccharomyces cerevisiae ) yEMC7 Uniprot Uniprot: P39543 Gene ( Saccharomyces...”
- Transmembrane protein 85 from both human (TMEM85) and yeast (YGL231c) inhibit hydrogen peroxide mediated cell death in yeast.
Ring, FEBS letters 2008 (PubMed)- GeneRIF: Overexpression of the yeast TMEM85 ortholog YGL231c leads to increased resistance to oxidative stress.
- PartCrafter: find, generate and analyze BioParts
Scher, Synthetic biology (Oxford, England) 2019 - “...SDD2 Protein with homology to human PDCD5. PDCD5 is involved in programmed cell death. 6 YGL231C EMC4 Member of conserved ER transmembrane complex. 7 YHR179W OYE2 Conserved NADPH oxidoreductase containing FMN. May be involved in sterol metabolism, oxidative stress response and programmed cell death. 8 YKL184W...”
- Widespread Cumulative Influence of Small Effect Size Mutations on Yeast Quantitative Traits
Hua, Cell systems 2018 - “...YCR034W, YMR292W, YDR233C, YNL297C, YGL005C, YDR245W, YBR036C, YDR221W, YPL192C, YLL014W, YDR508C, YEL001C, YER005W, YDR137W, YDL099W, YGL231C, YHR108W, YMR238W, YAL053W, YIL027C, YER072W, YML038C, YER120W, YEL027W, YIL030C, YDR492W, YJR131W, YMR010W, YHR181W, YPR063C, YIL124W, YLR350W, YJR088C, YBL011W, YML048W, YNL044W, YDR358W, YOR311C, YDR411C, YMR272C, YNL049C, YMR015C, YDL052C, YJR134C, YKL096W, YNL280C,...”
- Chemogenetic E-MAP in Saccharomyces cerevisiae for Identification of Membrane Transporters Operating Lipid Flip Flop
Vazquez, PLoS genetics 2016 - “...follows: Arrow 2: CHO1 interacting with Chr. VII bp 63048 to 202273, encompassing EMC4 (= YGL231C), OST5 , VRG4 , YIP4 , TPN1 , YIP5 and AIM14 (= YGL160W). Arrow 3, PCP1 interacting with Chr. XII bp 41280 to 211933 encompassing YBT1 (= YLL048C), GPI13 ,...”
- De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast Dekkera bruxellensis AWRI1499
Curtin, PloS one 2012 - “...detect around 10% of potential ORFs. Four CEGs (corresponding to S. cerevisae ORFs YGR181w, YOR103c, YGL231c, YGL047w) were detected at low identity or had only partial matches in the scaffold consensus. A further 11 CEGs with AWRI1499 gene model tblastn hits at worse than >1e-20 were...”
- Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum
Jonikas, Science (New York, N.Y.) 2009 (secret) - Transmembrane protein 85 from both human (TMEM85) and yeast (YGL231c) inhibit hydrogen peroxide mediated cell death in yeast
Ring, FEBS letters 2008 (PubMed)- “...both human (TMEM85) and yeast (YGL231c) inhibit hydrogen peroxide mediated cell death in yeast FEBS Letters Journal 00145793 582 17 2637 2642 2637-2642 17...”
- “...oxidative stress. Overexpression of the yeast TMEM85 ortholog (YGL231c) also leads to increased resistance to oxidative stress. Analysis of the existing TMEM85...”
- Meiotic recombination at the ends of chromosomes in Saccharomyces cerevisiae
Barton, Genetics 2008 - “...YFR039C YGL262Wh YGL257Ch YGL254W YGL253W YGL251C YGL248W YGL231C YGR288Wh YGR283C YGR266W YHL047Ch YHL046Ch YHL026C VIR (174) VIIL (164) VIIR (183)j VIIIL...”
- End resection initiates genomic instability in the absence of telomerase
Hackett, Molecular and cellular biology 2003 - “...to ADE5, 63,020 bp from the telomere, between YGL232w and YGL231c. URA3 was inserted 11 kb to the right of the centromere of chromosome VII, between MUQ1 and...”
- More
AT5G10780 hypothetical protein from Arabidopsis thaliana
Aligns to 51:166 / 187 (62.0%), covers 97.5% of PF06417, 136.3 bits
- TurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana
Feng, Nature plants 2023 - “...reticulum; Golgi; membrane Key player of retrieval of ER membrane proteins ASY3 Statistical analysis ATC30 AT5G10780 AT5G10780 endoplasmic reticulum; nucleus ER membrane protein complex subunit-like protein ASY3 Statistical analysis ATC31 AT5G53530 VPS26A membrane Homolog of yeast retromer subunit VPS26 ASY3 Statistical analysis ATC32 AT4G25050 ACP4 chloroplast;...”
- Looking for a safe haven: tail-anchored proteins and their membrane insertion pathways
Mehlhorn, Plant physiology 2021 - “...TAIR At5g28220 AtEMC2 b Cytosol c EMC3 At4g12590 AtEMC3 ER membrane c PSI-BLAST, TAIR EMC4 At5g10780 AtEMC4 ER membrane c PSI-BLAST, TAIR EMC5 At5g03345 PRCE2 ER membrane c PSI-BLAST, TAIR EMC6 At5g49540 AtEMC6 Plasma membrane c PSI-BLAST, TAIR EMC7 At2g25310 AtEMC7 a ER membrane c PSI-BLAST,...”
- Primers for low-copy nuclear genes in the Melastomataceae
Reginato, Applications in plant sciences 2016 - “...factor 1 50 R: YAGCTTTGACCGATCCRAGT UFP F: AAGTGGAGCGGGAAGGAGTA At4g06599 Ubiquitin family protein 53 R: RGGAGMCTCCACTTGGTCCA At5g10780 F: AGYGCCTTGCAGGGTGTTGG At5g10780 Unknown protein 50 R: RAGTAGGCCCAATGTGTTTA SOS4 F: GCAACAAGTCGGCTGTCTTT At5g37850 Pyridoxal kinase 51 R: CGGAGCTTCTTGACAACTTCC ZBADH F: TCCACGAGCTYGGGGGTRCC At5g61510 Zinc-binding alcohol dehydrogenase family 53 R: GGCCGGAAGAATCTGCTCTT waxy F1:...”
- “...Miconia included in this study. However, sequencing was problematic for some other samples of the At5g10780 marker (also in the Chaenanthera clade, not included in this study), due to a repetitive sequence of ca. 15 Ts. Sequences of the eight amplified loci for the samples of...”
PF3D7_1435400 ER membrane protein complex subunit 4, putative from Plasmodium falciparum 3D7
Aligns to 62:178 / 190 (61.6%), covers 96.6% of PF06417, 113.2 bits
cgd6_1070 hypothetical protein from Cryptosporidium parvum Iowa II
Aligns to 102:219 / 229 (51.5%), covers 94.1% of PF06417, 111.2 bits
EHI_063570 uncharacterized protein from Entamoeba histolytica HM-1:IMSS
Aligns to 1:117 / 135 (86.7%), covers 95.8% of PF06417, 86.4 bits
TGME49_259000 hypothetical protein from Toxoplasma gondii ME49
Aligns to 70:218 / 229 (65.1%), covers 94.1% of PF06417, 80.9 bits
7ktxD / P53073 Cryo-em structure of saccharomyces cerevisiae er membrane protein complex bound to a fab in ddm detergent (see paper)
Aligns to 5:62 / 74 (78.4%), covers 40.7% of PF06417, 48.6 bits
Or search for genetic data about PF06417 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory