Family Search for PF07577 (DUF1547)
PF07577 hits 6 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
CTA_0498 tarp protein (NCBI) from Chlamydia trachomatis A/HAR-13
4 alignments in 615:1031 / 1106 (21.2%), covering up to 100.0% of PF07577, 379.2 bits
TC0741 conserved hypothetical protein (NCBI ptt file) from Chlamydia muridarum Nigg
3 alignments in 532:817 / 1007 (17.7%), covering up to 100.0% of PF07577, 306.3 bits
- Protection against a chlamydial respiratory challenge by a chimeric vaccine formulated with the Chlamydia muridarum major outer membrane protein variable domains using the Neisseria lactamica porin B as a scaffold
Tifrea, NPJ vaccines 2020 - “...For example, Finco et al. 75 immunized mice with C. muridarum TC0106, TC0210, TC0313, or TC0741 and observed 0.50.9 log 10 reduction in the number of IFUs recovered from the lungs. However, vaccination with a combination of the four antigens resulted in a 4.1 log 10...”
- The Chlamydia pneumoniae Tarp Ortholog CPn0572 Stabilizes Host F-Actin by Displacement of Cofilin
Zrieq, Frontiers in cellular and infection microbiology 2017 - “...numbers are included): C. pneumoniae Cpn0572 CWL029 NP_224768.1 C. trachomatis Tarp LGV-434 AAT47185.1 C. muridarum TC0741 AAF39550 C. caviae CCA00170 AAP04921 Levels of similarity and identity between Cpn0572 and Tarp were calculated by EMBOSS http://emboss.bioinformatics.nl/cgi-bin/emboss/needle . Multiple sequence alignments of DUF domains from CPn0572 and its...”
- “...from CPn0572 and its chlamydial orthologs ( C. pneumoniae Cpn0572, C. trachomatis Tarp, C. muridarum TC0741 and C. caviae CCA00170) as shown by MultAlin. The first and last amino acids of each DUF are indicated on the left. The number of each amino acid residue within...”
- Update on Chlamydia trachomatis Vaccinology.
de, Clinical and vaccine immunology : CVI 2017 - “...muridarum challenge. Mice immunized with TC0106, TC0210, TC0313, or TC0741, adjuvanted with LTK63 plus CpG, had a 0.5 to 0.9 log10 reduction in the number of...”
- “...combinations (one combination was TC0106, TC0210, TC0313, and TC0741, and the other combination was TC0106, TC0431, TC0551, and TC0890) were also tested. The...”
- Outer membrane proteins preferentially load MHC class II peptides: implications for a Chlamydia trachomatis T cell vaccine
Karunakaran, Vaccine 2015 - “...Hypothetical protein TC0408 KLGKVLGPRNL I-E k TC0592 50S Ribosomal protein L1 RplA VTNKVTLAMQGQKLEG I-E k TC0741 Translocated actin recruiting phosphoprotein33 Tarp GSFAPVGLN I-A k TC0910 Hypothetical protein TC0910 Table 2 MHC class II (I-Ab)-bound C. trachomatis serovar D-derived peptides and their source proteins identified when C57BL/6...”
- Chlamydia muridarum T cell antigens and adjuvants that induce protective immunity in mice
Yu, Infection and immunity 2012 - “...TC0262 TC0261 TC0264 TC0801 TC0707 TC0190 TC0755 TC0741 TC0584 TC0420 TC0825 TC0285 Polymorphic membrane protein G Polymorphic membrane protein E/F-2...”
- Approach to discover T- and B-cell antigens of intracellular pathogens applied to the design of Chlamydia trachomatis vaccines
Finco, Proceedings of the National Academy of Sciences of the United States of America 2011 - “...in protection. TC0210, TC0313, TC0106, TC0551, and TC0741 from Combo1, Combo3, Combo4, and Combo5, respectively, induced IFU reductions ranging from 0.5-0.9...”
- “...belong to the group of the CD4+ T-cell inducers; TC0741 elicits high antibody titers in infected humans, and TC0210 induces both types of response. Recently...”
- Identification of immunodominant antigens by probing a whole Chlamydia trachomatis open reading frame proteome microarray using sera from immunized mice
Cruz-Fisher, Infection and immunity 2011 - “...case, for example, with TC0086 (OmpB), TC0263 (PmpG), TC0313, TC0741 (Tarp), and TC0715 (ClpP-1) (7, 17, 18, 32, 52, 65, 68). Some of these proteins, such as...”
- The conserved Tarp actin binding domain is important for chlamydial invasion
Jewett, PLoS pathogens 2010 - “...-Pro 740 ), (Thr 366 -Gln 666 ), (Leu 460 -Pro 740 ), C. muridarum (TC0741) (Glu 500 -Asp 600 ), (Thr 620 -Asp 720 ), (Asp 720 -Gly 820 ), (Asn 840 -Glu 940 ), (Ser 400 -Ala 827 ), (Glu 500 -Glu 940 ),...”
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CT456 hypothetical protein (NCBI ptt file) from Chlamydia trachomatis D/UW-3/CX
CT_456 translocated actin-recruiting phosphoprotein from Chlamydia trachomatis D/UW-3/CX
3 alignments in 629:930 / 1005 (17.4%), covering up to 100.0% of PF07577, 293.2 bits
- A primary Chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens
Randall, PloS one 2021 - “...recombinant C . trachomatis proteins. Six of the dominant antigens they identified (CT110, CT381, CT443, CT456, CT541 and CT681) are included in our list of dominant antigens. Follmann et al. [ 50 ], using a panel of 116 recombinant C . trachomatis serovar D proteins, screened...”
- Got mutants? How advances in chlamydial genetics have furthered the study of effector proteins.
Andersen, Pathogens and disease 2021 - “...CTL0709 IFA/inclusion membrane TargeTron/growth defect Unknown Unknown (Weber et al . 2015 , 2017 ) CT456 CTL0716 tarP Chlamydia BlaM assay/T3S dependent/cytosol near inclusion FRAEM, TargeTron/invasion defect Vinculin, FAK, Rac GEFs (Sos1, Vav2) Host cell invasion (Clifton etal . 2004 ; Jewett et al . 2006...”
- “...an anti-phosphotyrosine antibody coupled with mass spectrometry identified the presence of a bacterial effector protein, CT456, now designated t ranslocated a ctin- r ecruiting p hosphoprotein (TarP) (Clifton etal . 2004 ). Depending on the serovar, the N-terminal region of TarP contains 112 copies of a...”
- Sigma 54-Regulated Transcription Is Associated with Membrane Reorganization and Type III Secretion Effectors during Conversion to Infectious Forms of Chlamydia trachomatis.
Soules, mBio 2020 - “...CTL0619 hyp Putative inclusion membrane protein 94 2.98 CT392 c CTL0648 hyp Hypothetical 100 3.39 CT456 CTL0716 tarp Translocated actin-recruiting phosphoprotein 44 , 64 4.75 CT546 CTL0808 hyp Putative outer membrane protein 100 3.35 CT565 c CTL0828 hyp Putative membrane protein 78 3.36 CT576 CTL0839 scc2...”
- “...of the in vitro ATPase depletion study ( Fig.1 ). Interestingly, many 54 genes ( ct456 , ct619 , ct620 , ct683 , ct711 , and ct875 ) were transcribed at ratios twice those observed for the ATPase-defective domain (E242A) whereas rpoA levels were unchanged. This...”
- The Chlamydia trachomatis secreted effector TmeA hijacks the N-WASP-ARP2/3 actin remodeling axis to facilitate cellular invasion
Faris, PLoS pathogens 2020 - “...C . trachomatis tarP :: bla The intron was retargeted for C . trachomatis 434/Bu CT456 ( tarP ) using the TargeTron computer algorithm (TargeTronics). Insertion sites with the highest score and the closest proximity to the 5 ATG start codon were selected. The intron was...”
- “...transformed with each TargeTron construct as previously described [ 50 , 52 , 53 ]. CT456 was amplified with gene specific primers and sequenced to verify intron orientation. Coimmunoprecipitation HeLa cells were co-transfected with flag-tagged N-WASP or N-WASPGBD and GFP-tagged TmeA, TmeA GBD, TmeB, CT696, or...”
- Antibodies to Variable Domain 4 Linear Epitopes of the Chlamydia trachomatis Major Outer Membrane Protein Are Not Associated with Chlamydia Resolution or Reinfection in Women.
Collar, mSphere 2020 - “...10 5 60-kDa cysteine-rich OMP (OmcB) CT443 ATGPKQDSCFGRMY 8699 34 19/30 6 7 6 Tarp CT456 NIYESIGGSRTSGPEN 171184, 219234, 271284 b 30 14/30 3 6 5 Solute protein-binding family (metal ion transport protein) CT067 MNSCSSSRGNQPADESIY 2542 28 17/30 7 6 4 Putative outer membrane protein C...”
- “...available upon request by contacting the corresponding author. 10.1128/mSphere.00654-20.3 FIGS3 DSCB results for the Tarp (CT456) from sera of female patients with variable clinical outcomes. Amino acid positions from ca. 200 to 300 are a highly repetitive region in which the selected peptides cannot be resolved...”
- Fluorescence-Reported Allelic Exchange Mutagenesis-Mediated Gene Deletion Indicates a Requirement for Chlamydia trachomatis Tarp during In Vivo Infectivity and Reveals a Specific Role for the C Terminus during Cellular Invasion.
Ghosh, Infection and immunity 2020 (secret) - Transcriptional Expression of the ompA, cpaf, tarp, and tox Genes of Chlamydia trachomatis Clinical Isolates at Different Stages of the Developmental Cycle
Korhonen, Microorganisms 2019 - “...bacterial pathogenicity. Among the most extensively studied chlamydial effector proteins are translocated actin-recruiting phosphoprotein (Tarp, CT456), Chlamydia protease-like activity factor (CPAF; CT858) and chlamydial cytotoxin (CT166). Tarp is translocated into the host cell by the chlamydial type III secretion system and tyrosine phosphorylated by host kinases...”
- The multiple functions of the numerous Chlamydia trachomatis secreted proteins: the tip of the iceberg
Bugalhão, Microbial cell (Graz, Austria) 2019 - “...localization : inclusion lumen, cytosol and nucleus; function : unknown. [ 32 , 279 ] CT456 CTL0716 TarP Secretion : T3S; targets : actin, ABI1, VAV2, PI3K p85 subunit, SHC1; localization : cytosol, near the nascent inclusion, and membrane fractions of infected cells; function : actin...”
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- Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study.
Hufnagel, Microorganisms 2019 - “...of the positive samples. Besides CPAF other known immunogenic antigens such as pGP3, CT_681 (MOMP), CT_456 (TARP), and CT_110 (Hsp60) were also among the fifty most reactive antigens. 2. Exploration of Ct Antigens Related to Sequelae Of eleven TFI cases, nine (81.2%) were positive for antibodies...”
- “...26.9% 31 CT_687 cysteine desulfurase 14 9.7% 7 pGP3 virulence plasmid protein 37 25.5% 32 CT_456 translocated actin-recruting phosphoprotein TARP 14 9.7% 8 CT_104 enoyl-(acyl carrier protein) reductase 35 24.1% 33 CT_579 hypothetical protein 13 9.0% 9 CT_381 arginine ABC transporter substrate-binding protein ArtJ 35 24.1%...”
- Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression
Hufnagel, Scientific reports 2018 - “...Ct proteins CT_110 (HSP60, GroEL) 36 , CT_681 (major outer membrane protein, MOMP) 36 , CT_456 (translocated actin-recruiting phosphoprotein, TARP) 37 , CT_713 (outer membrane protein, PORB) 38 , and plasmid-encoded protein pGP3 13 as antigens. We analyzed sera from women with defined Ct-DNA status: Ct-DNA+(n=85)...”
- Following the Footsteps of Chlamydial Gene Regulation
Domman, Molecular biology and evolution 2015 - “...or membrane proteins ( fig. 6 ). These genes include the actin modulating effector TarP (CT_456) ( Clifton et al. 2004 ); the family of DUF582 proteins recently reported to be effectors (CT_620, CT_711, CT_712) ( Muschiol et al. 2011 ); a protein that interacts with...”
- Defining species-specific immunodominant B cell epitopes for molecular serology of Chlamydia species
Rahman, Clinical and vaccine immunology : CVI 2015 - “...E) CT_681 CT_812 CT_618 CT_119 CT_529 CT_442 CT_118 CT_443 CT_456 CT_116 65-86 33-87 23-72 12-80 31-88 20-88* 41-45 71-99 24-92 53-59 126 129 41 182 33 16...”
CTLon_0712 Translocated actin-recruiting protein (RefSeq) from Chlamydia trachomatis L2b/UCH-1/proctitis
2 alignments in 747:926 / 1001 (11.8%), covering up to 100.0% of PF07577, 185.4 bits
TARP_CHLT2 / Q6GX35 Translocated actin-recruiting phosphoprotein; Tarp protein from Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) (see paper)
YP_001654788 Translocated actin-recruiting phosphoprotein (RefSeq) from Chlamydia trachomatis 434/Bu
CTL0716 type III secretion system actin-recruiting effector Tarp from Chlamydia trachomatis 434/Bu
2 alignments in 751:930 / 1005 (11.7%), covering up to 100.0% of PF07577, 185.3 bits
- function: Appears to initiate or participate in signaling events that regulate the actin recruitment, which ultimately leads to internalization.
- Effector prediction in host-pathogen interaction based on a Markov model of a ubiquitous EPIYA motif
Xu, BMC genomics 2010 - “...Tir Escherichia Eoli AAC38390 EHIYDEVAA Y-474 Tir Citrobacter rodentium AAL06376 EPIYDEVAP Y-468 Trap Chlamydie trachomatis YP_001654788 ENIYENIYE Y-136 ENIYENIYE Y-238 ENIYENIYE Y-390 CagA: cytotoxin associated gene A [ 7 , 8 , 10 , 52 - 56 ]; BepD: Bartonella henselae protein D [ 14 -...”
- “...two motifs, i.e., Tir (EHIYDEVAA) motif and EPIYAKIQR, similar to the KK motif. Tarp protein (YP_001654788) of Chlamydia trachomatis contains not only the Tarp motif (ENIYENIYE), but also another motif ENIYESIDD, which is similar to the R4 motif. It reveals that although these proteins are not...”
- The Legionella pneumophila effector VipA is an actin nucleator that alters host cell organelle trafficking
Franco, PLoS pathogens 2012 - “...[22] . Accession numbers Swissprot IDs of proteins: Legionella pneumophila VipA, Q5C8M7; Chlamydia trachomatis TARP, Q6GX35; Salmonella enterica serovar typhimurium SipC, Q56020, and SipA, P0CL52; Vibrio parahaemolyticus VopL, B9A807, and Vibrio cholerae VopF, B5AN40; Rickettsia conorii Sca2, Q92JF7; Saccharomyces cerevisiae Bro1, P48582; Homo sapiens Alix, Q8WUM4....”
- Got mutants? How advances in chlamydial genetics have furthered the study of effector proteins.
Andersen, Pathogens and disease 2021 - “...IFA/inclusion membrane TargeTron/growth defect Unknown Unknown (Weber et al . 2015 , 2017 ) CT456 CTL0716 tarP Chlamydia BlaM assay/T3S dependent/cytosol near inclusion FRAEM, TargeTron/invasion defect Vinculin, FAK, Rac GEFs (Sos1, Vav2) Host cell invasion (Clifton etal . 2004 ; Jewett et al . 2006 ,...”
- Fluorescence-Reported Allelic Exchange Mutagenesis-Mediated Gene Deletion Indicates a Requirement for Chlamydia trachomatis Tarp during In Vivo Infectivity and Reveals a Specific Role for the C Terminus during Cellular Invasion.
Ghosh, Infection and immunity 2020 (secret) - Sigma 54-Regulated Transcription Is Associated with Membrane Reorganization and Type III Secretion Effectors during Conversion to Infectious Forms of Chlamydia trachomatis.
Soules, mBio 2020 - “...hyp Putative inclusion membrane protein 94 2.98 CT392 c CTL0648 hyp Hypothetical 100 3.39 CT456 CTL0716 tarp Translocated actin-recruiting phosphoprotein 44 , 64 4.75 CT546 CTL0808 hyp Putative outer membrane protein 100 3.35 CT565 c CTL0828 hyp Putative membrane protein 78 3.36 CT576 CTL0839 scc2 T3SS...”
- The multiple functions of the numerous Chlamydia trachomatis secreted proteins: the tip of the iceberg
Bugalhão, Microbial cell (Graz, Austria) 2019 - “...: inclusion lumen, cytosol and nucleus; function : unknown. [ 32 , 279 ] CT456 CTL0716 TarP Secretion : T3S; targets : actin, ABI1, VAV2, PI3K p85 subunit, SHC1; localization : cytosol, near the nascent inclusion, and membrane fractions of infected cells; function : actin nucleator;...”
- The Chlamydia psittaci genome: a comparative analysis of intracellular pathogens
Voigt, PloS one 2012 - “...(DUF1547) and an actin-binding I/LWEQ domain. Query coverage and sequence identity to C. trachomatis Tarp CTL0716 are 62% and 37%, respectively. This highlights the high degree of variability in these genes among the Chlamydiaceae . Even within C. trachomatis variation in the Tarp sequence has been...”
CCA_00170 type III secretion system actin-recruiting effector Tarp from Chlamydia caviae GPIC
CCA00170 conserved hypothetical protein (NCBI ptt file) from Chlamydophila caviae GPIC
Aligns to 569:619 / 880 (5.8%), covers 76.7% of PF07577, 82.1 bits
- Novel Chlamydia species isolated from snakes are temperature-sensitive and exhibit decreased susceptibility to azithromycin
Staub, Scientific reports 2018 - “...ompA Cpn_0695 C10C_0661 CCA_00047 C834K_0054 Cs308_0329/0497 T3SS Y Y Y Y Y tarp Cpn_0572 C10C_0530 CCA_00170 C834K_0183 Cs308_0200 tarp repeats N Y N N Y pmp genes 22 (6 pseudogenes) 18 18 (1 pseudogene) 19 (3 pseudogenes) 19 (2 pseudogenes) Locus_tags Cpn_0005, 0013, 0444-7, 0451, 0453-4,...”
- The Chlamydia pneumoniae Tarp Ortholog CPn0572 Stabilizes Host F-Actin by Displacement of Cofilin
Zrieq, Frontiers in cellular and infection microbiology 2017 - “...pneumoniae Cpn0572 CWL029 NP_224768.1 C. trachomatis Tarp LGV-434 AAT47185.1 C. muridarum TC0741 AAF39550 C. caviae CCA00170 AAP04921 Levels of similarity and identity between Cpn0572 and Tarp were calculated by EMBOSS http://emboss.bioinformatics.nl/cgi-bin/emboss/needle . Multiple sequence alignments of DUF domains from CPn0572 and its chlamydial orthologs were performed...”
- “...chlamydial orthologs ( C. pneumoniae Cpn0572, C. trachomatis Tarp, C. muridarum TC0741 and C. caviae CCA00170) as shown by MultAlin. The first and last amino acids of each DUF are indicated on the left. The number of each amino acid residue within the CPn0572 sequence is...”
- Tyrosine phosphorylation of the chlamydial effector protein Tarp is species specific and not required for recruitment of actin
Clifton, Infection and immunity 2005 - “...engineered with KpnI and BamHI linkers. Full-length Tarp (CCA00170) from C. caviae was similarly amplified and expressed as an in-frame GFP fusion. Clones of...”
- “...muridarum (MoPn) TC0741 (27), Chlamydophila caviae (GPIC) CCA00170 (28), and Chlamydophila pneumoniae (CWL029) (CPn) CPn0572 (22) were aligned using Clustal W,...”
Or search for genetic data about PF07577 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory