Family Search for PF08522 (DUF1735)
PF08522.10 hits 15 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
BT0865, BT_0865 putative chitobiase (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
2 alignments in 24:273 / 440 (53.6%), covering up to 84.2% of PF08522, 108.6 bits
- Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics
Yaung, Molecular systems biology 2015 - “...). At Day 7 in aerobic LB, enriched genes included metabolic enzymes, such as chitobiase (BT_0865), which degrades chitin, and stress response proteins, such as glycine betaine/Lproline transport system permease (BT_1750), which is involved in the import of osmoprotectants glycine betaine or proline that mitigate effects...”
- Sulfatases and a radical S-adenosyl-L-methionine (AdoMet) enzyme are key for mucosal foraging and fitness of the prominent human gut symbiont, Bacteroides thetaiotaomicron
Benjdia, The Journal of biological chemistry 2011 - “...only three genes, including a putative chitobiase (BT0865) and a periplasmic -glucosidase precursor (BT1872), exhibited significantly increased expression in...”
- “...to modify sulfated oligosaccharides. In contrast, the PUL BT0865- 67 exhibits dramatically diminished expression in the anSME strain, suggesting that the sensor...”
- Dissecting the in vivo metabolic potential of two human gut acetogens
Rey, The Journal of biological chemistry 2010 (no snippet)
BT4040 putative galactose oxidase precursor (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
2 alignments in 25:277 / 447 (54.1%), covering up to 90.8% of PF08522, 105.6 bits
BT1036 hypothetical protein (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
2 alignments in 29:301 / 449 (59.7%), covering up to 87.5% of PF08522, 101.4 bits
BT1037, BT_1037 conserved hypothetical protein (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
Aligns to 18:137 / 395 (30.4%), covers 98.3% of PF08522, 88.2 bits
BT3790 hypothetical protein (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
Aligns to 33:160 / 324 (39.5%), covers 95.0% of PF08522, 74.7 bits
BACOVA_03430 hypothetical protein (RefSeq) from Bacteroides ovatus ATCC 8483
Aligns to 30:161 / 344 (38.4%), covers 98.3% of PF08522, 73.3 bits
- Glycan complexity dictates microbial resource allocation in the large intestine
Rogowski, Nature communications 2015 - “...cell surface, likely to maximize efficiency of initial glycan capture. PUL-XylL encodes two potential SGBPs, BACOVA_03430 and BACOVA_03431. BACOVA_03430 did not bind to any of the xylans or xylooligosaccharides evaluated and thus is unlikely to be a SGBP. BACOVA_03431, a surface located lipoprotein ( Fig. 2...”
- “...MFS=transporter of the major facilitator superfamily (pink). Grey=unknown function (note, there is a structure of BACOVA_03430, PDB accession code 3N91). SusC-like (yellow) and SusD-like (light tan) proteins are a defining feature of PULs and are responsible for import of complex glycans across the outer membrane 18...”
BT1045, BT_1045 hypothetical protein (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
Aligns to 29:148 / 396 (30.3%), covers 93.3% of PF08522, 65.8 bits
BT2624 hypothetical protein (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
Aligns to 31:160 / 321 (40.5%), covers 98.3% of PF08522, 64.6 bits
BT3986 putative patatin-like protein (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
Aligns to 32:150 / 384 (31.0%), covers 98.3% of PF08522, 62.4 bits
- Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides
Trastoy, Nature communications 2020 - “...the released HM is held on the surface of B. thetaiotaomicron through the mannose-binding protein BT3986, while BT3984 recognizes the GlcNAc at the reducing end of the glycan and orientates the glycan into the outer membrane porin BT3983) for transport into the periplasm (Fig. 1 )...”
- “...in the cell surface. The HM product interacts with the surface glycan binding protein (SGBP), BT3986, and the SusD homolog BT3984 interacts with the reducing end GlcNAc, orientating the glycan into the SusC homolog BT3983 porin. The HM glycan is transported to the periplasm where it...”
- Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism
Cuskin, Nature 2015 - “.... The released HMNG is held on the surface of Bt through the mannose-binding protein BT3986, while the SusD homolog, BT3984, by recognizing the reducing end GlcNAc, Extended Data Fig. 7ef and Supplementary Table 5 , likely orientates the glycan into the outer membrane porin (SusC...”
- “...isothermal calorimetry titrations (ITCs) for BT3984 titrated with Gal-1,4-GlcNAc (LacNAc; (25 mM), and f, for BT3986 titrated with mannose (50 mM). The top half of each panel shows the raw ITC heats; the bottom half, the integrated peak areas fitted using a one single binding model...”
AAO79092.1 endo-β-N-acetylglucosaminidase (BT3987;BT_3987) (EC 3.2.1.96) (see protein)
Q8A0N4 Endo-beta-N-acetylglucosaminidase F1 from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
BT3987, BT_3987 endo-beta-N-acetylglucosaminidase F1 precursor (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
Aligns to 44:163 / 476 (25.2%), covers 99.2% of PF08522, 62.0 bits
- Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides
Trastoy, Nature communications 2020 - “...N -glycans and inactive against CT-type N -glycans. Comparison of BT3987 from B. thetaiotaomicron VPI-5482 (Q8A0N4, Uniprot code), EndoH from Streptomyces plicatus (P04067, Uniprot code), EndoF1 from Elizabethkingia meningoseptica (P36911, Uniprot code), A6286 from Prevotella melaninogenica (D9RSV7, Uniprot code), BT1285 from B. thetaiotaomicron VPI-5482 (Q8A889, Uniprot...”
- High resolution crystal structure of the endo-N-Acetyl-β-D-glucosaminidase responsible for the deglycosylation of Hypocrea jecorina cellulases
Stals, PloS one 2012 - “...Uniprot access code P36911.1), and Bacteroides thetaiotaomicron Endo BT (PDB ID 3POH, Uniprot access code Q8A0N4). The glycosylated Asn in the sequons of H. jecorina EndoT are shaded grey. Yellow shaded amino acids are the C-terminal residues observed in the respective crystal structures. The Endo T...”
- Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides
Trastoy, Nature communications 2020 - “...these species 7 . Fig. 1 Depolymerization of HM-type N -glycans by B. thetaiotaomicron . BT3987 deglycosylates HM-glycoproteins in the cell surface. The HM product interacts with the surface glycan binding protein (SGBP), BT3986, and the SusD homolog BT3984 interacts with the reducing end GlcNAc, orientating...”
- “...periplasm where it is processed by three mannosidases to the trisaccharide Man--1,6-Man-1,4-GlcNAc. Cartoon representation of BT3987 (PDB CODE 6T8I) and BT3990 (PDB CODE 2WVZ), and surface representation of BT3984 (PDB CODE 3CGH; 7). EndoBT-3987 is an endo-- N -acetylglucosaminidase (ENGase) that specifically catalyzes the hydrolysis of...”
- Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris
Seki, The Journal of biological chemistry 2019 (secret) - Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S
Trastoy, Nature communications 2018 - “...docking was performed using AutoDock Vina employing standard parameters 60 . Purification of EndoBT EndoBT (BT_3987 ( B. thetaiotaomicron VPI-5482)) in pSpeedET vector was purchased from DNASU plasmid repository ( https://dnasu.org/DNASU/Home.do ). EndoBT was expressed in BL21(DE3) (Novagen) in LB medium supplemented with 50lml 1 ampicillin....”
- Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism
Cuskin, Nature 2015 - “...requires GlcNAc at the reducing end for activity, producing Man-1,6-Man-1,4-GlcNAc, Extended Data Fig. 7b . BT3987 is a surface endo -acting N-acetylglucosaminidase, which cleaves the oligosaccharide from its polypeptide, Extended Data Fig. 7cd . The released HMNG is held on the surface of Bt through the...”
- “...in harness with HPAEC and fluorescence detection was used to measure the endo--N-acetylglucosaminidase activity of BT3987 against Man 9 GlcNAc 2 . The activity of the enzyme was also evaluated against glycoproteins using mass spectrometry using MALDI-TOF mass spectrometry as described below. BT2630 and BT3783 were...”
- Preferential packing of acidic glycosidases and proteases into Bacteroides outer membrane vesicles
Elhenawy, mBio 2014 - “...Acidic BT_3960 Cysteine proteinases Yes Acidic BT_1760 Levan hydrolase Yes Acidic BT_1737 -Amylase Yes Alkaline BT_3987 Endo-- N -acetylglucosaminidase Yes Acidic BT_3523 Glucanases Yes Acidic BT_3381 -Xylosidase Yes Acidic BT_1037 -Sialidase Yes Acidic BT_3745 Xylanase Yes Acidic BT_1761 Xylanase Yes Acidic BT_3985 Chitinase Yes Acidic BT_1038...”
- High resolution crystal structure of the endo-N-Acetyl-β-D-glucosaminidase responsible for the deglycosylation of Hypocrea jecorina cellulases
Stals, PloS one 2012 - “.... 279 : 22558 22570 . 5 Genomics JCFS (2010) Crystal structure of an endo-beta-N-acetylglucosaminidase BT_3987 from Bacteroides thetaiotaomicron at 1.55 resolution. (PDB ID 3POH).. 6 Stals I , Samyn B , Sergeant K , White T , Hoorelbeke K , et al ( 2010 )...”
- Structure of a SusD homologue, BT1043, involved in mucin O-glycan utilization in a prominent human gut symbiont
Koropatkin, Biochemistry 2009 - “...the SusC homolog, BT1042. Similarly, the BT3983-88 PUL, also encodes a predicted endo-- N -acetylglucosaminidase (BT3987). The mode of LacNAc binding to BT1043 is quite different from that observed by other LacNAc and/or O -glycan binding carbohydrate binding modules (CBMs). The crystal structures of the family...”
Ccan_17440 DUF1735 domain-containing protein from Capnocytophaga canimorsus Cc5
Aligns to 28:155 / 440 (29.1%), covers 85.8% of PF08522, 60.5 bits
- Glycan-foraging systems reveal the adaptation of Capnocytophaga canimorsus to the dog mouth
Renzi, mBio 2015 - “...modules ( 31 ) and one F5/8 type C ( 32 ) carbohydrate binding module. Ccan_17440 is the most abundant PUL -encoded protein of the C.canimorsus 5 surfome ( 15 ) and also harbors an F5/8 type C carbohydrate binding module. Ccan_17430 (mucinase) and Ccan_17450 (SusD-like)...”
- “...the eluate identified 35 proteins (see TableS1 in the supplemental material), among which were Ccan_17450, Ccan_17440, and Ccan_17430 . Thirteen proteins were identified in the mock eluate, of which 11 were also present in the Ccan_17470Strep-His eluate. No PUL9 -encoded product was identified in the mock...”
BT4407 conserved hypothetical protein (NCBI ptt file) from Bacteroides thetaiotaomicron VPI-5482
Aligns to 30:145 / 391 (29.7%), covers 95.8% of PF08522, 57.9 bits
Ccan_08730 DUF1735 domain-containing protein from Capnocytophaga canimorsus Cc5
Aligns to 31:169 / 441 (31.5%), covers 96.7% of PF08522, 56.5 bits
- The N-glycan glycoprotein deglycosylation complex (Gpd) from Capnocytophaga canimorsus deglycosylates human IgG
Renzi, PLoS pathogens 2011 - “...by ermF ; Em r [13] Cc5PUL5 Replacement of Ccan_08700 , Ccan_08710 , Ccan_08720 , Ccan_08730 by ermF : Em r Manfredi et al. , submitted Cc5gpdC Replacement of Ccan_08700 by ermF using primers 5073, 5074, 5075, 5083; Em r This study Cc5 gpdD Replacement of...”
- “...by ermF using primers 5001, 5002, 5005, 5006; Em r This study Cc5gpdE Replacement of Ccan_08730 by ermF using primers 5951, 5952, 5953, 5954; Em r This study Cc5gpdF Replacement of Ccan_08740 by ermF using primers 5955, 5956, 5957, 5958; Em r This study Growth of...”
D9RSV7 Putative endo-beta-N-acetylglucosaminidase H from Prevotella melaninogenica (strain ATCC 25845 / DSM 7089 / JCM 6325 / VPI 2381 / B282)
Aligns to 39:157 / 452 (26.3%), covers 80.0% of PF08522, 55.0 bits
- Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides
Trastoy, Nature communications 2020 - “...plicatus (P04067, Uniprot code), EndoF1 from Elizabethkingia meningoseptica (P36911, Uniprot code), A6286 from Prevotella melaninogenica (D9RSV7, Uniprot code), BT1285 from B. thetaiotaomicron VPI-5482 (Q8A889, Uniprot code), EF2863 from Enterococcus faecalis (Q830C5, Uniprot code), Endo-Fsp from Flavobacterium sp. (P80036, Uniprot code), Eng18B from Hypocrea atroviride IMI 206040...”
BACCELL_00844 hypothetical protein (RefSeq) from Bacteroides cellulosilyticus DSM 14838
Aligns to 54:185 / 490 (26.9%), covers 79.2% of PF08522, 42.8 bits
Or search for genetic data about PF08522 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory