Family Search for PF08719 (NADAR)

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Running HMMer for PF08719

PF08719 hits 21 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

RIBX_NOSP7 / B2J4E5 N-glycosidase Npun_R5314; Riboflavin biosynthesis intermediates N-glycosidase; EC 3.2.2.- from Nostoc punctiforme (strain ATCC 29133 / PCC 73102) (see paper)
Aligns to 4:146 / 156 (91.7%), covers 100.0% of PF08719, 148.5 bits

• function: Catalyzes the hydrolysis of the N-glycosidic bond in the first two intermediates of riboflavin biosynthesis, which are highly reactive metabolites, yielding relatively innocuous products. Thus, can divert a surplus of harmful intermediates into relatively harmless products and pre-empt the damage these intermediates would otherwise do. May act on other substrates in vivo. Has no activity against GTP, nucleoside monophosphates or ADP-ribose.
  catalytic activity: 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O = 2,5,6-triamino-4-hydroxypyrimidine + D-ribose 5-phosphate (RHEA:23436)
  catalytic activity: 5-amino-6-(5-phospho-D-ribosylamino)uracil + H2O = 5,6- diaminouracil + D-ribose 5-phosphate (RHEA:55020)

YbiA / b0798 N-glycosidase YbiA from Escherichia coli K-12 substr. MG1655 (see 3 papers)
ybiA / P30176 N-glycosidase YbiA from Escherichia coli (strain K12) (see 3 papers)
RIBX_ECOLI / P30176 N-glycosidase YbiA; Riboflavin biosynthesis intermediates N-glycosidase; EC 3.2.2.- from Escherichia coli (strain K12) (see 2 papers)
Aligns to 18:157 / 160 (87.5%), covers 98.8% of PF08719, 132.2 bits

• function: Catalyzes the hydrolysis of the N-glycosidic bond in the first two intermediates of riboflavin biosynthesis, which are highly reactive metabolites, yielding relatively innocuous products. Thus, can divert a surplus of harmful intermediates into relatively harmless products and pre-empt the damage these intermediates would otherwise do. Helps maintain flavin levels. May act on other substrates in vivo. Has no activity against GTP, nucleoside monophosphates or ADP-ribose (PubMed:25431972). Is Required for swarming motility (PubMed:17122336).
  catalytic activity: 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O = 2,5,6-triamino-4-hydroxypyrimidine + D-ribose 5-phosphate (RHEA:23436)
  catalytic activity: 5-amino-6-(5-phospho-D-ribosylamino)uracil + H2O = 5,6- diaminouracil + D-ribose 5-phosphate (RHEA:55020)
  disruption phenotype: Strongly represses cell swarming but no effect on cell swimming (PubMed:17122336). Cells lacking this gene show significantly reduced FAD and FMN levels (both by 13%) (PubMed:25431972).

RIBXA_VIBVY / Q7MGG3 Riboflavin biosynthesis protein VVA0006; EC 3.2.2.-; EC 3.5.4.25 from Vibrio vulnificus (strain YJ016) (see paper)
Aligns to 22:161 / 367 (38.1%), covers 100.0% of PF08719, 129.0 bits

• function: Catalyzes the hydrolysis of the N-glycosidic bond in the first two intermediates of riboflavin biosynthesis, which are highly reactive metabolites, yielding relatively innocuous products. Thus, can divert a surplus of harmful intermediates into relatively harmless products and pre-empt the damage these intermediates would otherwise do. Has no activity against GTP, nucleoside monophosphates or ADP- ribose.
  function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.
  catalytic activity: 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O = 2,5,6-triamino-4-hydroxypyrimidine + D-ribose 5-phosphate (RHEA:23436)
  catalytic activity: 5-amino-6-(5-phospho-D-ribosylamino)uracil + H2O = 5,6- diaminouracil + D-ribose 5-phosphate (RHEA:55020)
  catalytic activity: GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)- pyrimidine + formate + 3 H(+) + 2 phosphate (RHEA:23704)
  cofactor: Zn(2+) (Binds 1 zinc ion per subunit.)

RIBRX_MAIZE / K7WIZ6 Riboflavin biosynthesis protein PYRR, chloroplastic; EC 1.1.1.193; EC 3.2.2.- from Zea mays (Maize) (see 2 papers)
Aligns to 456:610 / 623 (24.9%), covers 98.8% of PF08719, 121.9 bits

• function: Pyrimidine reductase involved in the riboflavin biosynthesis pathway. Has also a non-functional N-terminal deaminase domain that lacks the catalytically essential zinc-binding residues. 39% activity when NADH replaces NADPH. No evidence for a phosphatase activity conferred by the N-terminal domain.
  function: Catalyzes the hydrolysis of the N-glycosidic bond in the first two intermediates of riboflavin biosynthesis, which are highly reactive metabolites, yielding relatively innocuous products. Thus, can divert a surplus of harmful intermediates into relatively harmless products and pre-empt the damage these intermediates would otherwise do. Has no activity against GTP, nucleoside monophosphates or ADP- ribose.
  catalytic activity: 5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5- amino-6-(5-phospho-D-ribosylamino)uracil + H(+) + NADPH (RHEA:17845)
  catalytic activity: 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O = 2,5,6-triamino-4-hydroxypyrimidine + D-ribose 5-phosphate (RHEA:23436)
  catalytic activity: 5-amino-6-(5-phospho-D-ribosylamino)uracil + H2O = 5,6- diaminouracil + D-ribose 5-phosphate (RHEA:55020)
  disruption phenotype: Lethal when homozygous. Required for both endosperm and embryo formation in the seed.

PHS1 / F4JBI5 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193) from Arabidopsis thaliana (see paper)
Aligns to 427:583 / 596 (26.3%), covers 98.8% of PF08719, 121.5 bits

RIBRX_ARATH / Q9STY4 Riboflavin biosynthesis protein PYRR, chloroplastic; EC 1.1.1.193; EC 3.2.2.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Aligns to 448:586 / 599 (23.2%), covers 67.5% of PF08719, 86.6 bits

• function: Pyrimidine reductase involved in the riboflavin biosynthesis pathway. Has also a non-functional N-terminal deaminase domain that lacks the catalytically essential zinc-binding residues.
  function: Catalyzes the hydrolysis of the N-glycosidic bond in the first two intermediates of riboflavin biosynthesis, which are highly reactive metabolites, yielding relatively innocuous products. Thus, can divert a surplus of harmful intermediates into relatively harmless products and pre-empt the damage these intermediates would otherwise do. Helps maintain flavin levels. Has no activity against GTP, nucleoside monophosphates or ADP-ribose.
  catalytic activity: 5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5- amino-6-(5-phospho-D-ribosylamino)uracil + H(+) + NADPH (RHEA:17845)
  catalytic activity: 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O = 2,5,6-triamino-4-hydroxypyrimidine + D-ribose 5-phosphate (RHEA:23436)
  catalytic activity: 5-amino-6-(5-phospho-D-ribosylamino)uracil + H2O = 5,6- diaminouracil + D-ribose 5-phosphate (RHEA:55020)

Or search for genetic data about PF08719 in the Fitness Browser