Family Search for PF08933 (PrnB)

PF08933 hits 8 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

SO4414 conserved domain protein from Shewanella oneidensis MR-1
Aligns to 5:391 / 392 (98.7%), covers 99.7% of PF08933, 696.3 bits

• 3D-Fun: predicting enzyme function from structure
  von, Nucleic acids research 2008
  • “...4 ). Example number 2 presents the functional annotation for 1ZEE of the hypothetical protein SO4414 from Shewanella oneidensis , which has been deposited by the NSGC ( Table 1 ). This protein is a member of the PFAM DUF1864 family, which still remains to be...”
• Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase
  Forouhar, Proceedings of the National Academy of Sciences of the United States of America 2007
  • “...(PDB entry 1YW0) (15). The structure of the SO4414 protein from Shewanella oneidensis (16) was determined at 2.4-A resolution by molecular replacement based on...”
  • “...the Xanthomonas campestris TDO and a related protein SO4414 from Shewanella oneidensis, including the structure at 1.6-A resolution of the catalytically active,...”

2nwbA / Q8E972 Crystal structure of a putative 2,3-dioxygenase (so4414) from shewanella oneidensis in complex with ferric heme. Northeast structural genomics target sor52. (see paper)
Aligns to 1:374 / 379 (98.7%), covers 99.7% of PF08933, 646.0 bits

• Ligand: protoporphyrin ix containing fe (2nwbA)

WP_011061882 PrnB family protein from Pseudomonas protegens Pf-5
PFL_3605 pyrrolnitrin biosynthesis enzyme PrnB from Pseudomonas fluorescens Pf-5
Aligns to 14:360 / 361 (96.1%), covers 96.9% of PF08933, 396.9 bits

• Preliminary crystallographic characterization of PrnB, the second enzyme in the pyrrolnitrin biosynthetic pathway.
  De, Acta crystallographica. Section F, Structural biology and crystallization communications 2006
  • GeneRIF: PrnB was overproduced in Pseudomonas fluorescens and well diffracting crystals were obtained.
• Bacterial subfamily of LuxR regulators that respond to plant compounds
  Subramoni, Applied and environmental microbiology 2011
  • “...most likely part of an operon that also includes PFL_3605 (prnB). PFL_3607, another gene in this cluster, showed only a 1.3-fold increase in the CHA0PsoR...”

Pchl3084_3145 monodechloroaminopyrrolnitrin synthase PrnB family protein from Pseudomonas chlororaphis subsp. aureofaciens 30-84
Aligns to 14:360 / 361 (96.1%), covers 96.9% of PF08933, 376.9 bits

• The Phenazine 2-Hydroxy-Phenazine-1-Carboxylic Acid Promotes Extracellular DNA Release and Has Broad Transcriptomic Consequences in Pseudomonas chlororaphis 30-84
  Wang, PloS one 2016
  • “...Ratios * (O * /ZN) Exoenzymes and Secondary metabolites Pchl3084_3146 prnA tryptophan halogenase 2.8 3.4 Pchl3084_3145 prnB pyrrolnitrin biosynthesis 2.6 3.0 Pchl3084_3144 prnC halogenase 2.6 3.0 Pchl3084_3143 prnD aminopyrrolnitrin oxidase 2.6 3.6 Pchl3084_2021 chiC chitinase 2.6 5.5 Pchl3084_3180 chiC chitinase 6.1 17.6 Pchl3084_3127 aprA metalloprotease 2.6...”
• Roles of the Gac-Rsm pathway in the regulation of phenazine biosynthesis in Pseudomonas chlororaphis 30-84
  Wang, MicrobiologyOpen 2013
  • “...Pchl3084_3143 prnD Aminopyrrolnitrin oxidase 6.94 0.57 0.08 0.04 Pchl3084_3144 prnC Halogenase 12.53 0.85 0.07 0.02 Pchl3084_3145 prnB Pyrrolnitrin biosynthesis 6.88 1.23 0.18 0.00 Pchl3084_3146 prnA Tryptophan halogenase 16.45 1.83 0.11 0.01 Chitinase Pchl3084_2020 Chitin-binding domain protein 36.06 4.28 0.12 0.03 Pchl3084_2021 Chitinase 31.17 3.46 0.11 0.01...”

prnB / P95481 monodechloroaminopyrrolnitrin synthase from Pseudomonas fluorescens (see 2 papers)
PRNB_PSEFL / P95481 Monodechloroaminopyrrolnitrin synthase PrnB; 7-chloro-L-tryptophan dioxygenase PrnB; EC 1.14.19.- from Pseudomonas fluorescens (see 4 papers)
Aligns to 14:360 / 361 (96.1%), covers 96.1% of PF08933, 368.5 bits

• function: Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin. Catalyzes the ring rearrangement and decarboxylation to convert 7-chloro-L-tryptophan (7-CLT) to monodechloroaminopyrrolnitrin (MDA). It can also use 7-chloro-D-tryptophan, but 7-chloro-L-tryptophan is the preferred natural enantiomer.
  catalytic activity: 7-chloro-L-tryptophan + AH2 + O2 = monodechloroaminopyrrolnitrin + A + CO2 + 2 H2O (RHEA:50952)
  cofactor: heme b
  subunit: Monomer.
  disruption phenotype: Cells lacking this gene lose the ability to produce pyrrolnitrin.

CXP47_RS17635, EY04_RS17645 monodechloroaminopyrrolnitrin synthase PrnB family protein from Pseudomonas chlororaphis
Aligns to 14:359 / 360 (96.1%), covers 96.6% of PF08933, 368.0 bits

• Investigation of the quorum-sensing regulon of the biocontrol bacterium Pseudomonas chlororaphis strain PA23
  Shah, PloS one 2020
  • “...- 458 atcgGCTACCTGTTCTCGCTGTtcgt Tryptophan halogenase PrnA cl26176 Q EY04_RS17650 -4.17 -1.93 378 PrnB - - EY04_RS17645 -5.61 - FAD-dependent oxidoreductase cl27554 - EY04_RS17640 -5.92 -2.63 2Fe-2S ferredoxin cl28556 - EY04_RS17635 -6.64 -1.86 Flavin reductase - - EY04_RS17630 -3.58 - Potassium transporter COG0475 P EY04_RS17625 -4.42 -...”
• Genome Sequence of Pseudomonas chlororaphis Lzh-T5, a Plant Growth-Promoting Rhizobacterium with Antimicrobial Activity
  Li, Genome announcements 2018
  • “...antibiotics, and gene clusters for antibiotics synthesis were found. For example, prnA through prnD (CXP47_RS17640, CXP47_RS17635, CXP47_RS17630, and CXP47_RS17625) coding for pyrrolnitrin ( 11 ), hcnA through hcnC (CXP47_RS12080, CXP47_RS12085, and CXP47_RS12090) coding for volatile compound hydrogen cyanide ( 12 ), and phzI , phzR ,...”

2x67A / P95481 The ternary complex of prnb (the second enzyme in pyrrolnitrin biosynthesis pathway), tryptophan and cyanide (see paper)
Aligns to 11:350 / 350 (97.1%), covers 95.3% of PF08933, 365.0 bits

• Ligands: protoporphyrin ix containing fe; tryptophan (2x67A)

NL30_32795 monodechloroaminopyrrolnitrin synthase PrnB family protein from Burkholderia contaminans
Aligns to 14:359 / 361 (95.8%), covers 95.1% of PF08933, 356.5 bits

• Comparative genome-wide analysis reveals that Burkholderia contaminans MS14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis
  Deng, MicrobiologyOpen 2016
  • “...biosynthetic gene cluster (Agnoli etal. 2006 ), respectively. On the chromosome three, the genes NL30_32800, NL30_32795, NL30_32790, and NL30_32785 share an overall 90% nucleotide sequence similarity to the prnABCD genes in pyrrolnitrin biosynthetic gene cluster (Costa etal. 2009 ). In addition, two PKS genes NL30_36200 and...”

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