Family Search for PF10021 (DUF2263)
PF10021.9 hits 11 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
D1AC29 poly(ADP-ribose) glycohydrolase (EC 3.2.1.143) from Thermomonospora curvata (see paper)
Tcur_1721 hypothetical protein (RefSeq) from Thermomonospora curvata DSM 43183
Aligns to 47:197 / 316 (47.8%), covers 100.0% of PF10021, 181.2 bits
SCO0909 hypothetical protein (NCBI) from Streptomyces coelicolor A3(2)
Aligns to 59:199 / 329 (42.9%), covers 100.0% of PF10021, 177.1 bits
- The WblC/WhiB7 Transcription Factor Controls Intrinsic Resistance to Translation-Targeting Antibiotics by Altering Ribosome Composition
Lee, mBio 2020 - “...SCO4388 (D). (E and F) Examples of antisense RNAs originated from UTRs of the neighboring SCO0909 (E) and SCO1988 (F) genes. Download FIGS2, PDF file, 0.4 MB . Copyright 2020 Lee et al. 2020 Lee et al. This content is distributed under the terms of the...”
- ADP-ribosylation: new facets of an ancient modification
Palazzo, The FEBS journal 2017 - “...process in Streptomycescoelicolor ADPribosyltransferases SCO2860 (Arr homologue) SCO3953 (Tpt1/KptA homologue) SCO5461 (Pierisin homologue) Macrodomain hydrolases SCO0909 (bacterialtype PARG) SCO6450 (MacroD homologue) SCO6735 (TARG1like) DraG/ARH like hydrolases SCO0086 SCO1766 SCO2028 SCO2029 SCO2030 SCO2031 SCO4435 SCO5809 Sirtuins SCO0452 SCO6464 John Wiley & Sons, Ltd This article is being...”
- “...are macrodomain proteins representing three different classes within the macrodomain superfamily (Table 1 ). The SCO0909 is a bacterialtype PARG that cleaves the PARylation in the same manner as mammalian PARGs 36 . Nothing is known about the function of SCO0909, but in the radiationresistant bacterium...”
- Disruption of Macrodomain Protein SCO6735 Increases Antibiotic Production in Streptomyces coelicolor
Lalić, The Journal of biological chemistry 2016 - “...We identified both DraG-like (SCO0086, SCO1766, SCO2028, SCO2029, SCO2030, SCO4435, and SCO5809) and macrodomain containing (SCO0909, SCO6450, and SCO6735) hydrolases. Two of three macrodomain proteins have predicted functions. The SCO0909 protein of S. coelicolor is a bacterial-type PARG, predicted to remove poly-ADP-ribosylation ( 6 ); whereas...”
- “...DraG homologues (SCO0086, SCO1766, SCO2028, SCO2029, SCO2030, SCO4435, and SCO5809), whereas three are macrodomain proteins (SCO0909, SCO6450, and SCO6735), representing three different groups of macrodomain superfamily. Although in eukaryotes macrodomains exist as compositions with other domains forming functionally complex proteins like ALC1, PARP9, and macroH2A1 (...”
alr1372 hypothetical protein (NCBI ptt file) from Nostoc sp. PCC 7120
Aligns to 7:159 / 278 (55.0%), covers 100.0% of PF10021, 170.4 bits
SNA_17125 TIGR02452 family protein from Streptomyces natalensis ATCC 27448
Aligns to 9:162 / 295 (52.2%), covers 100.0% of PF10021, 166.5 bits
Haur_1618 hypothetical protein (RefSeq) from Herpetosiphon aurantiacus ATCC 23779
Aligns to 10:154 / 271 (53.5%), covers 99.3% of PF10021, 165.8 bits
DRB0099 hypothetical protein (NCBI ptt file) from Deinococcus radiodurans R1
DR_B0099 TIGR02452 family protein from Deinococcus radiodurans R1
Aligns to 10:159 / 285 (52.6%), covers 100.0% of PF10021, 152.8 bits
- ATP-type DNA ligase requires other proteins for its activity in vitro and its operon components for radiation resistance in Deinococcus radiodurans in vivo
Kota, Biochemistry and cell biology = Biochimie et biologie cellulaire 2010 (PubMed)- “...copy of drB0100 along with other genes such as drb0099 and drb0098, organized downstream in the operon. The in vitro DNA ligase activity of LigB was stimulated...”
- “...did not improve when PprA was substituted with purified DRB0099 protein or when DRB0098 protein was substituted with the DRB0099 protein in the presence of PprA...”
- [Construction of the gene drb0099 deleted mutant and adversity analysis in the extremely radioresistant bacterium Deinococcus radiodurans]
Chang, Wei sheng wu xue bao = Acta microbiologica Sinica 2008 (PubMed)- “...Deinococcus radiodurans R1 has extraordinary resistance to radiation. DRB0099 might play an important role in protecting the bacterium against radiation. To verify the inference, we deleted drb0099 and constructed the mutant. Comparing with the wild type, the mutant grew...”
- “...lower with UV treatment time increased, the survival fraction was much higher than that of drb0099. The wild type could repair DNA double strands breaks better than the mutant. The gene drb0099 might directly relate with the DNA repair system. The mutant was more sensitive to...”
- Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans
Blasius, BMC molecular biology 2007 - “...addition, DRB0100 belongs to a putative DNA repair operon together with the genes DRB0098 and DRB0099. DRB0098 has been predicted to encode a kinase/phosphatase with an unusual domain architecture [ 9 ] whereas DRB0099 is classified as a domain of unknown function with weak similarity to...”
- “...and a polynucleotide kinase domain and resembles the human repair protein PNKP [ 27 ]; DRB0099 is an open reading frame with unknown function and weak similarity to macro domains [ 8 , 10 ]. No DNA ligation was detected with any of these three operon...”
- Comparative genomics of Thermus thermophilus and Deinococcus radiodurans: divergent routes of adaptation to thermophily and radiation resistance
Omelchenko, BMC evolutionary biology 2005 - “...DRB0098, a protein containing an HD family phosphatase and polynucleotide kinase domains [ 7 ]; DRB0099, a predicted phosphatase of the H2Macro superfamily ([ 27 ] and KSM, unpublished observations); a double-stranded DNA-binding protein PprA (DRA0346), which stimulates the DNA end-joining reaction in vitro [ 77...”
- Conservation and diversity of radiation and oxidative stress resistance mechanisms in Deinococcus species
Lim, FEMS microbiology reviews 2019 - “...2 1 1 1 1 1 1 1 1 DR_B0100 1 1 1 1 1 DR_B0099 1 1 DR_B0098 1 1 1 1 1 DR_B0094 1 1 1 DR_B0095 fr 1 Other DNA repair proteins TopA 1 1 1 2 3 2 2 1 1 1...”
- “...(Liu etal. 2003 ). DR_B0100 is the first gene of a radiation-induced operon also encoding DR_B0099 (poly ADP-ribose glycohydrolase) and DR_B0098 (polynucleotide kinase) (Blasius etal. 2007 ; Slade etal. 2011 ). A DR_B0100 mutant is IR resistant as the wild type according to one study (Makarova...”
- N 4-Cytosine DNA Methylation Is Involved in the Maintenance of Genomic Stability in Deinococcus radiodurans
Li, Frontiers in microbiology 2019 - “...2.42E-26 DNA damage repair protein, PprA DNA repair proteins DR_1262 7.4276 1.96E-155 Ribonucleoprotein Ro/SS-A-like protein DR_B0099 3.3137 5.88E-18 Poly ADP-ribose glycohydrolase, PARG DR_1297 3.09 6.24E-12 Uncharacterized membrane protein, MutK DR_0221 2.7957 1.35E-07 Endonuclease domain-containing protein (DUF559) DR_0596 2.6105 2.51E-14 DNA helicase subunit, RuvB DR_1902 2.6449 2.96E-11...”
- Deletion of the rnl gene encoding a nick-sealing RNA ligase sensitizes Deinococcus radiodurans to ionizing radiation
Schmier, Nucleic acids research 2017 - “...8 ). LigB is encoded by the first ORF in a three-gene operon that includes DR_B0099 , a poly ADP-ribose glycohydrolase ( 9 ), and DR_B0098 , a 5-OH polynucleotide kinase ( 7 ). This operon is transcriptionally upregulated at 1.5 to 12 h after exposure...”
- The structure and catalytic mechanism of a poly(ADP-ribose) glycohydrolase
Slade, Nature 2011 - “...pColdTF vector (Takara). The A. fumigatus Af293 gene (AFUA_4G11940), the D. radiodurans R1 ATCC13939 gene (DR_B0099) and the E. dispar SAW760 gene (EDI_110590) were amplified from genomic DNA, the human PARP1 and PARG gene (Q86W56) were amplified from HeLa cDNA, while the T. curvata DSM 43183...”
T3D766 DUF2263 domain-containing protein from Clostridioides difficile CD160
QEW_4455 TIGR02452 family protein from Clostridioides difficile CD160
Aligns to 10:154 / 255 (56.9%), covers 97.9% of PF10021, 122.0 bits
- Comparative inhibitory profile and distribution of bacterial PARPs, using Clostridioides difficile CD160 PARP as a model
García-Saura, Scientific reports 2018 - “...inhibitor KU-0058948 17 . In addition, H. aurantiacus also has a DUF2263 protein (UniProt code: T3D766) that is capable of effectively removing PAR 17 , and whose sequence contains a poly (ADP-Ribose) glycohydrolase (PARG) signature (GGG-X 68 QEE) 18 . Thus, the presence of both enzymes...”
- “...was abolished by recombinant protein corresponding to the Clostridioides difficile CD160 QEW_4455 gene (Uniprot code: T3D766). This uncharacterized protein has a DUF2263 domain similar to that of hPARG and HaPARG, which also reverses PARylation (Supplementary Fig. S4 ). These results demonstrate that C. difficile CD160 has...”
- Comparative inhibitory profile and distribution of bacterial PARPs, using Clostridioides difficile CD160 PARP as a model
García-Saura, Scientific reports 2018 - “...by hPARP1 and CdPARP was abolished by recombinant protein corresponding to the Clostridioides difficile CD160 QEW_4455 gene (Uniprot code: T3D766). This uncharacterized protein has a DUF2263 domain similar to that of hPARG and HaPARG, which also reverses PARylation (Supplementary Fig. S4 ). These results demonstrate that...”
- “...M. Pascal (Universit de Montral, Montral, QC) in a pET24b vector. PARP (QEW_4625) and PARG (QEW_4455) genes from Clostridioides difficile CD160 (BioProject and SRA Accession numbers PRJNA85757 and SRR593185, respectively) were obtained from GenScript (Piscataway, USA). Genomic DNA from Herpetosiphon aurantiacus DSM785, purchased from DSMZ (Braunschweig,...”
AFUA_2G02500 conserved hypothetical protein from Aspergillus fumigatus Af293
Aligns to 13:143 / 271 (48.3%), covers 79.5% of PF10021, 80.5 bits
- RNAseq analysis of Aspergillus fumigatus in blood reveals a just wait and see resting stage behavior
Irmer, BMC genomics 2015 - “...U3 small nucleolar ribonucleoprotein protein 2.23 AFUA_3G06380 exosome-associated family protein 2.24 AFUA_4G14180 hypothetical protein 2.25 AFUA_2G02500 hypothetical protein 2.26 1.60 AFUA_5G04010 tRNA-splicing endonuclease subunit Sen2 2.32 AFUA_2G02970 hypothetical protein 2.34 AFUA_6G03750 amidophosphoribosyltransferase 2.38 AFUA_8G01850 phosphate-repressible phosphate permease 2.43 AFUA_8G05720 DUF567 domain protein 2.44 AFUA_4G14190 hypothetical protein...”
AFUA_4G11940 conserved hypothetical protein from Aspergillus fumigatus Af293
Aligns to 14:201 / 335 (56.1%), covers 69.2% of PF10021, 70.6 bits
- The structure and catalytic mechanism of a poly(ADP-ribose) glycohydrolase
Slade, Nature 2011 - “...N-terminal truncation (1-455) was expressed from the pColdTF vector (Takara). The A. fumigatus Af293 gene (AFUA_4G11940), the D. radiodurans R1 ATCC13939 gene (DR_B0099) and the E. dispar SAW760 gene (EDI_110590) were amplified from genomic DNA, the human PARP1 and PARG gene (Q86W56) were amplified from HeLa...”
LRC_03250 TIGR02452 family protein from Lactobacillus ruminis ATCC 27782
Aligns to 8:139 / 257 (51.4%), covers 77.4% of PF10021, 70.5 bits
- Lactobacillus ruminis strains cluster according to their mammalian gut source
O', BMC microbiology 2015 - “...LRC_00800 3.18 * 0.74 >0.05 5.00 0.2 (0.19-0.21) 0.19 (0.16-0.23) PTS system fructose-specific MMOD 5 LRC_03250 2.62 >0.05 0.60 >0.05 9.00 17.92 (17.39-18.46) 0.22 (0.18-0.27) hypothetical protein MMOD 6 LRC_04370 0.96 >0.05 1.00 >0.05 1.03 1.06 (1.01-1.10) 0.22 (0.18-0.26) hypothetical_protein MMOD 9 LRC_05780 4.04 ** 0.89...”
K0N7J8 DUF2263 domain-containing protein from Lactobacillus paracasei
Aligns to 4:137 / 252 (53.2%), covers 79.5% of PF10021, 69.8 bits
Or search for genetic data about PF10021 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory