Family Search for PF10228 (DUF2228)
PF10228 hits 7 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
HPF1_HUMAN / Q9NWY4 Histone PARylation factor 1 from Homo sapiens (Human) (see 18 papers)
NP_060337 histone PARylation factor 1 from Homo sapiens
Aligns to 77:328 / 346 (72.8%), covers 100.0% of PF10228, 369.9 bits
- function: Cofactor for serine ADP-ribosylation that confers serine specificity on PARP1 and PARP2 and plays a key role in DNA damage response (PubMed:28190768, PubMed:29480802, PubMed:29954836, PubMed:33186521, PubMed:32028527, PubMed:32939087, PubMed:34486521, PubMed:34874266, PubMed:34210965, PubMed:34625544, PubMed:33589610, PubMed:34732825, PubMed:33683197, PubMed:34795260, PubMed:34108479). Initiates the repair of double-strand DNA breaks: recruited to DNA damage sites by PARP1 and PARP2 and switches the amino acid specificity of PARP1 and PARP2 from aspartate or glutamate to serine residues, licensing serine ADP-ribosylation of target proteins (PubMed:28190768, PubMed:29480802, PubMed:29954836, PubMed:32028527, PubMed:32939087, PubMed:34486521, PubMed:34874266, PubMed:34625544, PubMed:33589610, PubMed:34732825, PubMed:33683197, PubMed:34795260). Serine ADP- ribosylation of target proteins, such as histones, promotes decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (PubMed:27067600, PubMed:28190768, PubMed:32939087, PubMed:33589610). Serine ADP- ribosylation of proteins constitutes the primary form of ADP- ribosylation of proteins in response to DNA damage (PubMed:29480802). HPF1 acts by completing the active site of PARP1 and PARP2: forms a composite active site composed of residues from HPF1 and PARP1 or PARP2 (PubMed:32028527, PubMed:33589610). While HPF1 promotes the initiation of serine ADP-ribosylation, it restricts the polymerase activity of PARP1 and PARP2 in order to limit the length of poly-ADP-ribose chains (PubMed:34732825, PubMed:33683197, PubMed:34795260). HPF1 also promotes tyrosine ADP-ribosylation, probably by conferring tyrosine specificity on PARP1 (PubMed:29954836, PubMed:30257210).
subunit: Interacts with PARP1 (via the PARP catalytic domain) (PubMed:27067600, PubMed:32028527, PubMed:33589610). Interacts with PARP2 (via the PARP catalytic domain) (PubMed:27067600, PubMed:33141820, PubMed:34108479, PubMed:32028527, PubMed:32939087). Interacts with core nucleosomes in a PARP1- and PARP2-dependent manner (PubMed:27067600, PubMed:32939087). - Slow Dissociation from the PARP1-HPF1 Complex Drives Inhibitor Potency.
Stojanovic, Biochemistry 2023 - “...PARP1HPF1 complex ( PDF ) Supplementary Material bi3c00243_si_001.pdf Accession Codes PARP1, UniProtKB P09874. HPF1, UniProtKB: Q9NWY4. Author Contributions P.S. performed most of the assays and data analysis. J.R. performed some of the assays, designed and optimized the assay format, and assisted with the data analysis. P.S.,...”
- Hyperthermia enhances the efficacy of chemotherapeutic drugs in heat-sensitive cells through interfering with DNA damage repair.
Ni, Annals of translational medicine 2022 - “...cells treated with chemotherapy alone (group B) Protein accession Gene name Protein description D/B ratio Q9NWY4 HPF1 Histone PARylation factor 1 0.8315 A6NHR9 SMCHD1 Structural maintenance of chromosomes flexible hinge domain-containing protein 1 0.831 P50851 LRBA Lipopolysaccharide-responsive and beige-like anchor protein 0.8215 Q12873 CHD3 Chromodomain-helicase-DNA-binding protein...”
- Alleviation of a polyglucosan storage disorder by enhancement of autophagic glycogen catabolism
Kakhlon, EMBO molecular medicine 2021 - “...DR1 associated protein 1(DRAP1) + Q9UBB4 Ataxin 10(ATXN10) + Q96A33 Coiledcoil domain containing 47(CCDC47) + Q9NWY4 Histone PARylation factor 1(HPF1) + Q9UI26 Importin 11(IPO11) + a A functional assembly which includes at least one protein and other macromolecules and/or ligands. b Small, spherical organelles enclosed by...”
- Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome.
Bacic, eLife 2021 - “...Q86WJ1 Recombinant DNA reagent Human HPF1 (1346) pET28a (plasmid) Gaullier et al., 2020 UniProt ID Q9NWY4 Recombinant DNA reagent Human PARP1 (11014) pET28a (plasmid) Langelier et al., 2017 UniProt ID P09874 Recombinant DNA reagent Human PARP2 Isoform 2 (1-570) pET28a (plasmid) GenScript UniProt ID Q9UGN5 Software,...”
- HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase.
Rudolph, eLife 2021 - “...PARP1 UniProt P09874 As described in Rudolph et al., 2018 Peptide, recombinant protein HPF1 UniProt Q9NWY4 As described in manuscript Sequenced-based reagent p18mer DNA IDT 5-phosphate-GGGTTGCGGCCGCTTGGG-3; double-stranded Strain, strain background ( Escherichia coli ) Rosetta DE3 pLys EMD Millipore 70956 Chemically competent cells Other 384-well plates...”
- Bridging of DNA breaks activates PARP2-HPF1 to modify chromatin.
Bilokapic, Nature 2020 - “...inverse PCR (iPCR) using plasmids that contained the gene for the full-length protein: HPF1 (UniProt Q9NWY4) and PARP2 (UniProt Q9UGN52). iPCR reactions were set up in a total volume of 25 l. 10 l of purified PCR product was incubated with 5 U of T4 PNK...”
- Brain Proteomic Profiling in Intractable Epilepsy Caused by TSC1 Truncating Mutations: A Small Sample Study.
Liu, Frontiers in neurology 2020 - “...protein gamma (PURG) 0.007938768 2.38 Nucleus P15559 NAD(P)H dehydrogenase [quinone] 1 (NQO1) 0.03367561 2.4 Cytoplasm Q9NWY4 Histone PARylation factor 1 (HPF1) 0.044527851 2.55 P54105 Methylosome subunit pICln (CLNS1A) 0.045372858 2.56 Cytoplasm/Nucleus O00273 DNA fragmentation factor subunit alpha (DFFA) 0.019867376 2.58 Cytoplasm Q96BW5 Phosphotriesterase-related protein (PTER) 0.010206613...”
- Establishment of neutralizing rat monoclonal antibodies for fibroblast growth factor-2.
Tanaka, Monoclonal antibodies in immunodiagnosis and immunotherapy 2014 - More
- HPF1 remodels the active site of PARP1 to enable the serine ADP-ribosylation of histones.
Sun, Nature communications 2021 - GeneRIF: HPF1 remodels the active site of PARP1 to enable the serine ADP-ribosylation of histones.
- HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase.
Rudolph, eLife 2021 - GeneRIF: HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase.
- Serine-linked PARP1 auto-modification controls PARP inhibitor response.
Prokhorova, Nature communications 2021 - GeneRIF: Serine-linked PARP1 auto-modification controls PARP inhibitor response.
- The regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome.
Hendriks, Nature communications 2021 - GeneRIF: The regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome.
- Dual function of HPF1 in the modulation of PARP1 and PARP2 activities.
Kurgina, Communications biology 2021 - GeneRIF: Dual function of HPF1 in the modulation of PARP1 and PARP2 activities.
- HPF1 dynamically controls the PARP1/2 balance between initiating and elongating ADP-ribose modifications.
Langelier, Nature communications 2021 - GeneRIF: HPF1 dynamically controls the PARP1/2 balance between initiating and elongating ADP-ribose modifications.
- HPF1 completes the PARP active site for DNA damage-induced ADP-ribosylation.
Suskiewicz, Nature 2020 - GeneRIF: HPF1 completes the PARP active site for DNA damage-induced ADP-ribosylation; as HPF1 forms a joint active site with PARP1 or PARP2, our data implicate HPF1 as an important determinant of the response to clinical PARP inhibitors
- Bridging of DNA breaks activates PARP2-HPF1 to modify chromatin.
Bilokapic, Nature 2020 - GeneRIF: Bridging of DNA breaks activates PARP2-HPF1 to modify chromatin.
- More
Q8CFE2 Histone PARylation factor 1 from Mus musculus
Aligns to 77:328 / 346 (72.8%), covers 100.0% of PF10228, 365.8 bits
HPF1_DANRE / Q7SXS8 Histone PARylation factor 1 from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
Aligns to 80:329 / 348 (71.8%), covers 100.0% of PF10228, 358.4 bits
- function: Cofactor for serine ADP-ribosylation that confers serine specificity on parp1 and parp2 and plays a key role in DNA damage response. Initiates the repair of double-strand DNA breaks: recruited to DNA damage sites by parp1 and parp2 and switches the amino acid specificity of parp1 and parp2 from aspartate or glutamate to serine residues, licensing serine ADP-ribosylation of target proteins. Serine ADP-ribosylation of target proteins, such as histones, promotes decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Hpf1 acts by completing the active site of parp1 and parp2: forms a composite active site composed of residues from Hpf1 and parp1 or parp2. While hpf1 promotes the initiation of serine ADP-ribosylation, it restricts the polymerase activity of parp1 and parp2 in order to limit the length of poly-ADP-ribose chains. Hpf1 also promotes tyrosine ADP-ribosylation, probably by conferring tyrosine specificity on parp1.
subunit: Interacts with PARP1 (via the PARP catalytic domain). Interacts with PARP2 (via the PARP catalytic domain). Interacts with core nucleosomes in a parp1- and parp2-dependent manner.
disruption phenotype: Morpholino knockdown of the protein causes malformation anbd delayed hatching.
A7RS11 Histone PARylation factor 1 from Nematostella vectensis
Aligns to 62:310 / 327 (76.1%), covers 100.0% of PF10228, 339.6 bits
B7P8X7 Histone PARylation factor 1 from Ixodes scapularis
Aligns to 99:347 / 372 (66.9%), covers 100.0% of PF10228, 307.7 bits
CG1218, NP_649589 uncharacterized protein from Drosophila melanogaster
Aligns to 176:428 / 449 (56.3%), covers 100.0% of PF10228, 287.3 bits
- HPF1/C4orf27 Is a PARP-1-Interacting Protein that Regulates PARP-1 ADP-Ribosylation Activity
Gibbs-Seymour, Molecular cell 2016 - “...in more detail. We identified a protein in Drosophila melanogaster harboring a PBZ domain, termed CG1218 ( Figure1 A) ( Ahel etal., 2008 , Isogai etal., 2010 ). The human homolog of CG1218 is the uncharacterized protein C4orf27. C4orf27 is highly conserved in metazoans but is...”
- A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance
Valakh, Developmental biology 2012 - “...present the phenotype of RNAi knockdown of a zinc finger domain-containing uncharacterized protein encoded by CG1218 that has an ~50% increase in the number of boutons compared to control (p<0.001; n=19 NMJs from 6 animals). For synaptic retractions , there are two enriched groups including six...”
- “...( UAS-DCR2/+; Elav-Gal4/+ ) (B) CG2807 RNAi ( UAS-DCR2/+; UAS-CG2807 RNAi/+; Elav-Gal4/+ ) and (C) CG1218 RNAi ( UAS-DCR2/+; UAS-CG1218 RNAi/+; Elav-Gal4/+ ) third instar larvae stained for the presynaptic active zone protein Brp (red) and the postsynaptic glutamate receptor DGluRIII (green). Knockdown of spliceosome factor...”
- Solution structure of a zinc-finger domain that binds to poly-ADP-ribose
Isogai, Genes to cells : devoted to molecular & cellular mechanisms 2010 (PubMed)- GeneRIF: the three-dimensional structure of the poly-ADP-ribose (pADPr)-binding zinc-finger domain from Drosophila melanogaster CG1218-PA
- Developmental expression patterns of candidate cofactors for vertebrate six family transcription factors
Neilson, Developmental dynamics : an official publication of the American Association of Anatomists 2010 - “...genes ( Gadd45 , Gadd45 ) and one gene each with homology to CG9083, CG17265, CG1218, CG1135, CG7878, CG33090, RhoGEF2, Chc, CKII, CG10576, VhaAC39, RhoGAP93B , and RpS27 ( Table 1 ). Xenopus candidate Six-interacting genes are expressed in developing craniofacial tissues Xenopus Six1 is initially...”
- “...as an EST with homology to only a very short region of the Drosophila protein (CG1218), which reduced our confidence that this is a true orthologue. As summarized in Table 2 , we found that the candidate Xenopus Six-interacting genes representing 11 of the Drosophila SO-interacting...”
- Solution structure of a zinc-finger domain that binds to poly-ADP-ribose.
Isogai, Genes to cells : devoted to molecular & cellular mechanisms 2010 (PubMed)- GeneRIF: the three-dimensional structure of the poly-ADP-ribose (pADPr)-binding zinc-finger domain from Drosophila melanogaster CG1218-PA
- Integrating computational biology and forward genetics in Drosophila
Aerts, PLoS genetics 2009 - “...allowed us to perform knockdown of 17 genes. Of these, 2 genes ( CG1024 , CG1218 ,) caused lethality only in combination with atoRNAi , but not under control conditions ( Table 4 ). As a further confirmation for the specificity of these interactions, we tested...”
- “...ato 1 CGRNAi+hGal4, ato 1 CGRNAi+hGal4 18597 CG1024 7.7% Lethal No Effect No Effect 31685 CG1218 6.6% Lethal No Effect No Effect Results of the phenotypes observed from RNAi screen. An Atonal Interaction Network The combination of forward and reverse genetics tools and computational biology allowed...”
XP_036010205 histone PARylation factor 1 isoform X3 from Mus musculus
Aligns to 1:169 / 187 (90.4%), covers 62.9% of PF10228, 201.2 bits
Or search for genetic data about PF10228 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory