Family Search for PF10728 (DUF2520)
PF10728 hits 13 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
3d1lB / Q5LAQ6 Crystal structure of putative NADP oxidoreductase bf3122 from bacteroides fragilis
Aligns to 131:256 / 259 (48.6%), covers 100.0% of PF10728, 142.0 bits
- Ligand: 2-mercapto-propion aldehyde (3d1lB)
PG0659 conserved hypothetical protein from Porphyromonas gingivalis W83
Aligns to 136:261 / 270 (46.7%), covers 100.0% of PF10728, 130.4 bits
DET0801 pyrroline-5-carboxylate reductase, putative from Dehalococcoides ethenogenes 195
Aligns to 132:261 / 285 (45.6%), covers 98.4% of PF10728, 127.2 bits
CBU_1660 hypothetical protein from Coxiella burnetii RSA 493
Aligns to 138:266 / 294 (43.9%), covers 97.6% of PF10728, 118.2 bits
EF1861 conserved domain protein from Enterococcus faecalis V583
Aligns to 132:258 / 281 (45.2%), covers 98.4% of PF10728, 116.9 bits
MAP_0455 DUF2520 domain-containing protein from Mycobacterium avium subsp. paratuberculosis K-10
MAP0455 hypothetical protein from Mycobacterium avium subsp. paratuberculosis str. k10
Aligns to 145:287 / 303 (47.2%), covers 99.2% of PF10728, 111.8 bits
- The Fur-like regulatory protein MAP3773c modulates key metabolic pathways in Mycobacterium avium subsp. paratuberculosis under in-vitro iron starvation
Thapa, Scientific reports 2024 - “...and gabT (4-aminobutyrate transaminase) for Pantothenate synthesis. Among them panB, panC, panD , coax and MAP_0455 were downregulated in MAP 3773c strain at 30min post-iron stress. ( c ) Network analysis of downregulated genes in MAP3773c strain at 30min post-iron starvation involved in glycosyltransferases, polysacaharide and...”
- The Fur-like regulatory protein MAP3773c modulates key metabolic pathways in Mycobacterium avium subsp. paratuberculosis under in-vitro iron starvation
Thapa, Scientific reports 2024 - “...interactions between four genes in the pan operon ( panB , panC , panD and MAP0455 ) with coax and gabT (4-aminobutyrate transaminase, an enzyme involved in alanine and aspartate metabolism) (Fig. 5 b). Figure 5 Pantothenate (Pan) and Coenzyme (CoA) biosynthesis pathway in MAP. (...”
- “...STRING ver. 11.5. Network analysis showed interaction of six genes panB, panC, panD , coax, MAP0455 and gabT (4-aminobutyrate transaminase) for Pantothenate synthesis. Among them panB, panC, panD , coax and MAP_0455 were downregulated in MAP 3773c strain at 30min post-iron stress. ( c ) Network...”
2i76A / Q9X250 Crystal structure of protein tm1727 from thermotoga maritima
Aligns to 112:240 / 247 (52.2%), covers 88.0% of PF10728, 108.9 bits
- Ligand: nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (2i76A)
MAB_0540 hypothetical protein from Mycobacterium abscessus ATCC 19977
Aligns to 164:306 / 326 (43.9%), covers 98.4% of PF10728, 107.9 bits
- Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification
Zhang, Frontiers in public health 2024 - “...clofazimine-resistant M. abscessus strains, revealing several significant mutations in the MAB_2299c , MAB_1483 , and MAB_0540 genes, which were found to play crucial roles in conferring drug resistance. The identification of the erm41 gene and its pivotal role in macrolide resistance has facilitated the critical differentiation...”
- Epidemiology of Nontuberculous Mycobacteria in Nanjing and MAB_0540 Mutations Associated with Clofazimine Resistance in Mycobacterium abscessus
Zhang, Infection and drug resistance 2023 - “...Resistance 1178-6973 Dove 10171220 408986 10.2147/IDR.S408986 Original Research Epidemiology of Nontuberculous Mycobacteria in Nanjing and MAB_0540 Mutations Associated with Clofazimine Resistance in Mycobacterium abscessus Zhang et al Zhang et al Zhang Ruixian 1 Luo Sha 1 Wang Nan 1 Zhang Hongying 2 http://orcid.org/0000-0002-4896-0209 Wu Xuping 1...”
- “...of M. abscessus and M. intracellulare . Furthermore, we determined whether MAB_1448, MAB_4384, MAB_2299c, MAB_1483, MAB_0540, rplD, rplC , and rrl were related to drug resistance to provide an experimental basis for the use of these five drugs in the treatment of NTM. Methods We identified...”
- Nontuberculous Mycobacterial Resistance to Antibiotics and Disinfectants: Challenges Still Ahead
Tarashi, BioMed research international 2022 - “...Some mutations related to clofazimine resistance were also identified in M. abscessus , such as MAB_0540, MAB_2299c, and MAB_1483 [ 80 ]. M. intracellulare also showed some mutations in the mmpL5 gene. However, M. avium isolates, despite being clofazimine-resistant, did not show any related mutations [...”
- Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
Dohál, Microorganisms 2021 - “...of M. abscessus . Their results revealed several high-confidence gene mutations (in MAB_2299c, MAB_1483, and MAB_0540 genes) involved in resistance to this drug, whose use is increasingly preferred due to limited treatment options [ 77 ]. In addition, M. abscessus subsp. abscessus possesses the erm gene,...”
- Current Molecular Therapeutic Agents and Drug Candidates for Mycobacterium abscessus
Quang, Frontiers in pharmacology 2021 - “...using 29 laboratory-induced CFZ-resistant Mab strains through whole-genome sequencing, wherein three genes (MAB_2299c, MAB_1483 and MAB_0540) were identified to be most commonly associated with CFZ resistance. However, future studies are needed to address the role of the identified mutations ( Chen et al., 2018 ). CFZ...”
- Novel Mutations Associated with Clofazimine Resistance in Mycobacterium abscessus
Chen, Antimicrobial agents and chemotherapy 2018 - “...a possible transcriptional regulatory protein), MAB_1483, and MAB_0540 are most commonly associated with clofazimine resistance. In addition, mutations in...”
- “...We conclude that mutations in MAB_2299c, MAB_1483, and MAB_0540 are the major mechanisms of clofazimine resistance in M. abscessus. Future studies are needed to...”
- Identification of novel mutations associated with clofazimine resistance inMycobacterium abscessus
Chen, 2018
O06279 Conserved hypothetical alanine and leucine rich protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
BCG_3667c hypothetical alanine and leucine rich protein from Mycobacterium bovis BCG str. Pasteur 1173P2
Rv3603c CONSERVED HYPOTHETICAL ALANINE AND LEUCINE RICH PROTEIN from Mycobacterium tuberculosis H37Rv
Aligns to 145:287 / 303 (47.2%), covers 99.2% of PF10728, 107.3 bits
- A d-Phenylalanine-Benzoxazole Derivative Reveals the Role of the Essential Enzyme Rv3603c in the Pantothenate Biosynthetic Pathway of Mycobacterium tuberculosis
Pepi, ACS infectious diseases 2022 - “..., 17 . C-terminally His-tagged variants of Mtb IlvC (UniProt: P9WKJ7) and Mtb PanG (UniProt: O06279) were produced from codon-optimized genes using pET28a(+) expression vectors (GenScript, Piscataway, NJ). The sequences are available in Supporting Table S4 . Mtb IlvC was expressed in E. coli BL21(DE3) and...”
- A noninvasive BCG skin challenge model for assessing tuberculosis vaccine efficacy
Krishnan, PLoS biology 2024 (no snippet) - Mammalian lectin arrays for screening host-microbe interactions
Jégouzo, The Journal of biological chemistry 2020 - “...origin of replication, with a hygromycin-resistance cassette and a panCD operon driven by the constitutive BCG_3667c promoter, followed by the multiple-cloning site ( 60 ). Mycobacteria were grown at 37 C in Middlebrook 7H9 broth (BD Biosciences) supplemented with 0.05% Tween 80 (Sigma), 0.2% glycerol (Sigma),...”
- Analysis of Genome-Wide Mutational Dependence in Naturally Evolving Mycobacterium tuberculosis Populations
Green, Molecular biology and evolution 2023 - “...protein MoeB2 3894732 Rv3478 ppe60 Host immune response ( Su et al. 2018 ) 4046007 Rv3603c Rv3603c Conserved hypothetical alanine- and leucine-rich protein 4060588 Rv362 c esxW Influencing increased transmission in Beijing lineage ( Holt et al. 2018 ) 4338371 Rv3862c whiB6 Transcriptional regulator with known...”
- The Global Success of Mycobacterium tuberculosis Modern Beijing Family Is Driven by a Few Recently Emerged Strains
Zhu, Microbiology spectrum 2023 - “...4215793 A/C Rv3770c : Hypothetical leucine-rich protein Rv3770c : Probable remnant of a transposase L2.3.6 Rv3603c 4046007 C/T V38M Conserved hypothetical alanine- and leucine-rich protein DISCUSSION In this study, we used phylogenetic branching and population dynamics to demonstrate that L2.3 (modern Beijing) diversified into six phylogenetic...”
- A d-Phenylalanine-Benzoxazole Derivative Reveals the Role of the Essential Enzyme Rv3603c in the Pantothenate Biosynthetic Pathway of Mycobacterium tuberculosis
Pepi, ACS infectious diseases 2022 - “...35015509 9558617 10.1021/acsinfecdis.1c00461 NIHMS1839722 Article A D-Phenylalanine-Benzoxazole Derivative Reveals the Role of the Essential Enzyme Rv3603c in the Pantothenate Biosynthetic Pathway of Mycobacterium tuberculosis Pepi Michael J. 1 Chacko Shibin 2 Marqus Gary M. 1 Singh Vinayak 3 Wang Zhe 4 Planck Kyle 4 Cullinane Ryan...”
- “...glutamicum , but Mtb IlvC does not display KPR activity. We identified the essential protein Rv3603c as an ortholog of PanG KPR, and demonstrated that purified recombinant Rv3603c has KPR activity. Q112 inhibits Rv3603c, explaining the metabolomic changes. Surprisingly, pantothenate does not rescue Q112 -treated bacteria,...”
- Vitamin in the Crosshairs: Targeting Pantothenate and Coenzyme A Biosynthesis for New Antituberculosis Agents
Butman, Frontiers in cellular and infection microbiology 2020 - “...( Ec PanK). The other, Mt CoaX, is a type III PanK encoded by coaX (Rv3603c), which is found in an open reading frame with panD and panC . However, unlike other well-characterized type III PanKs such as the enzymes from Pseudomonas aeruginosa and Bacillus anthracis...”
- Comprehensive Characterization of the Attenuated Double Auxotroph Mycobacterium tuberculosisΔleuDΔpanCD as an Alternative to H37Rv
Mouton, Frontiers in microbiology 2019 - “...play a role in dormancy, oxidative and/or nitrosative stress (AhpC, AhpD), pantothenate metabolism (CoaX, PanB, Rv3603c), transcription (Rv2989), methyl transfer (Rv2003c) and lipid catabolism (Rv2037c). This, in addition to increased abundance of DevR in M. tuberculosis leuD panCD during growth at pH 6.5, may suggest that...”
- Mutations in panD encoding aspartate decarboxylase are associated with pyrazinamide resistance in Mycobacterium tuberculosis
Zhang, Emerging microbes & infections 2013 - “...the 5 individual mutants. panD is located in an operon lysS-Rv3603c with panC (pantothenate synthetase), Rv3603c (conserved hypothetical alanine and leucine rich protein), Rv3600c (hypothetical protein), Rv3559c (hypothetical protein ), and lysS (lysyl-tRNA synthetase 1) ( Figure 2 ). panD encodes a 139 amino acid (15kD)...”
- Annotation of the M. tuberculosis hypothetical orfeome: adding functional information to more than half of the uncharacterized proteins
Doerks, PloS one 2012 - “...(A) Species tree with family representatives and corresponding operon architecture. COG4595 (containing the hypothetical gene rv3603c (red unit)) evidentially linked to several genes of pantothenate (vitamine B5) biosynthesis pathway (variably colored units upstream the red unit) and to folic acid (vitamine B9) biosynthesis pathway (downstream the...”
- “...synthetase is an attractive chemotherapeutic target [36] . In our genomic context analysis we detect rv3603c (member of COG5495), a gene of unknown function, in a conserved operon with panC and other genes of the pantothenate biosynthesis pathway ( Figure 4a and b ). Interestingly, the...”
- Exploring drug action on Mycobacterium tuberculosis using affymetrix oligonucleotide genechips
Fu, Tuberculosis (Edinburgh, Scotland) 2006 - “...0.001927 Rv3362c Probable ATP/GTP-binding protein 2.3 0.000122 Rv3603c Conserved hypothetical alanine and leucine rich protein 3.5 0.000429 Table 3 Functional...”
A9762_03120 DUF2520 domain-containing protein from Pandoraea sp. ISTKB
Aligns to 146:275 / 310 (41.9%), covers 96.8% of PF10728, 102.7 bits
FTN_1351 hypothetical protein from Francisella tularensis subsp. novicida U112
Aligns to 118:232 / 238 (48.3%), covers 85.6% of PF10728, 55.4 bits
FTL_0675 hypothetical protein from Francisella tularensis subsp. holarctica
FTS_0676 2-dehydropantoate 2-reductase PanG from Francisella tularensis subsp. holarctica FSC200
Aligns to 117:231 / 237 (48.5%), covers 85.6% of PF10728, 54.0 bits
- Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis
Horzempa, BMC microbiology 2008 - “...46 , 69 ] FTL_0672 Aspartate-1-decarboxylase, panD 2.0 [ 14 , 46 , 69 ] FTL_0675 Conserved hypothetical protein 2.3 [ 46 , 69 ] FTL_0837 D-methionine binding transport protein, ABC transporter, membrane and periplasmic protein, metlQ 2.0 [ 13 ] FTL_0885 PhoH-like protein 1.9 [...”
- The Multiple Localized Glyceraldehyde-3-Phosphate Dehydrogenase Contributes to the Attenuation of the Francisella tularensis dsbA Deletion Mutant
Pavkova, Frontiers in cellular and infection microbiology 2017 - “...AFT92755 0920 Hypothetical protein FTS_0920 up- S Brotcke et al., 2006 AFT92560 0676 Hypothetical protein FTS_0676 up- S AFT92796 0974 Hypothetical protein FTS_0974 up- * S Ren et al., 2014 AFT92765 0935 GTP-dependent nucleic acid-binding protein EngD up- * J AFT92195 0200 Hypothetical protein FTS_0200 down-...”
panG / Q5NF59 2-dehydropantoate 2-reductase (EC 1.1.1.169) from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) (see paper)
FTT_1388 hypothetical protein from Francisella tularensis subsp. tularensis SCHU S4
Aligns to 118:232 / 238 (48.3%), covers 85.6% of PF10728, 54.0 bits
Or search for genetic data about PF10728 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory