Family Search for PF11685 (DUF3281)
April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.
PF11685 hits 7 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
FTN_0573 hypothetical protein from Francisella tularensis subsp. novicida U112
Aligns to 4:270 / 271 (98.5%), covers 100.0% of PF11685, 615.1 bits
- Molecular complexity orchestrates modulation of phagosome biogenesis and escape to the cytosol of macrophages by Francisella tularensis
Asare, Environmental microbiology 2010 - “...FTN_0439 protein of unknown function 4 # tnfn1_pw060418p01q142 FTN_0482 protein of unknown function 6 tnfn1_pw060328p04q110 FTN_0573 protein of unknown function 2 tnfn1_pw060418p02q126 FTN_0573 protein of unknown function 4 tnfn1_pw060328p08q173 FTN_0584 araJ conserved inner membrane protein of unknown function 5 tnfn1_pw060510p04q147 FTN_0599 protein of unknown function 2...”
- Molecular bases of proliferation of Francisella tularensis in arthropod vectors
Asare, Environmental microbiology 2010 - “...FTN_0439 protein of unknown function 4 # tnfn1_pw060418p01q142 FTN_0482 protein of unknown function 6 tnfn1_pw060328p04q110 FTN_0573 protein of unknown function 2 tnfn1_pw060418p02q126 FTN_0573 protein of unknown function 4 tnfn1_pw060328p08q173 FTN_0584 araJ conserved inner membrane protein of unknown function 5 tnfn1_pw060510p04q147 FTN_0599 protein of unknown function 2...”
- Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis
Horzempa, BMC microbiology 2008 - “...for F. novicida genes important for a murine infection, the tivA allele of F. novicida (FTN_0573) was not identified [ 14 ]. There are two possible explanations for this discrepancy. There may be intrinsic differences between F. tularensis LVS and F. novicida pathogenesis [ 43 ]...”
- “...forms (FTN_1103, 52% identity; FTN_1101, 49% identity) may have compensated for a mutation of tivA (FTN_0573) during the negative-selection screen [ 14 ], thereby masking any effects on pathogenesis. This possibility underscores the value of analyzing several different strains when probing F. tularensis pathogenesis. Strain 1664d,...”
FTL_1581 hypothetical lipoprotein from Francisella tularensis subsp. holarctica
Aligns to 4:270 / 271 (98.5%), covers 100.0% of PF11685, 612.3 bits
- Identification of disulfide bond isomerase substrates reveals bacterial virulence factors
Ren, Molecular microbiology 2014 - “...limited genetic screen by randomly selecting and independently deleting six hypothetical proteins, FTL_0424, FTL_0878, FTL_1548, FTL_1581, FTL_1678, and FTL_1709, that contained more than three cysteines. Of the these six hypothetical proteins, two were found to be required for F. tularensis virulence in a mouse pulmonary infection...”
- Francisella-arthropod vector interaction and its role in patho-adaptation to infect mammals
Akimana, Frontiers in microbiology 2011 - “...for initial mammalian infection (Schwan et al., 1995 ; Tilly et al., 2006 ). Alternatively, FTL_1581, a hypothetical lipoprotein induced by mammalian temperature (Horzempa et al., 2008 ) could have a similar role as OspC. In addition, F. tularensis ssp. novicida has been shown to alter...”
- Working toward the future: insights into Francisella tularensis pathogenesis and vaccine development
Pechous, Microbiology and molecular biology reviews : MMBR 2009 - “...katG FTT0708 FTL_1542 FTL_1553 migR sucC FTL_1554 sucD FTL_1581 FTL_1583 tivA xasA FTL_1601 FTL_1622 FTL_1623 FTL_1664 yibK FTT0444 FTT0443 deoB FTL_1670 dsbB...”
- Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis
Horzempa, BMC microbiology 2008 - “...generated two mutants of loci induced at 37C [FTL_1581 and FTL_1664 ( deoB )]. The FTL_1581 mutant was attenuated in a chicken embryo infection model, which was likely attributable to a defect in survival within macrophages. FTL_1581 encodes a novel hypothetical protein that we suggest naming...”
- “...of temperature-regulated genes also defines a rich collection of novel candidate virulence determinants, including tivA (FTL_1581). An analysis of tivA and deoB (FTL_1664) revealed that these genes contribute to intracellular survival and entry into mammalian cells, respectively. Background Francisella tularensis is a Gram-negative bacterium that is...”
L1103_FRATN / A0Q6X4 Bacterial lipoprotein FTN_1103; BLP from Francisella tularensis subsp. novicida (strain U112) (see paper)
FTN_1103 hypothetical protein from Francisella tularensis subsp. novicida U112
Aligns to 10:281 / 282 (96.5%), covers 99.6% of PF11685, 291.7 bits
- function: Stimulates the host immune inflammatory signaling system allowing the host to combat the bacteria. Stimulates mouse interleukin- 6 (Il6) production (PubMed:23584588).
disruption phenotype: Significantly decreases interleukin-6 (Il6) production in a Tlr2-dependent fashion by C57BL/6 mouse BMDM. - Pathogenicity and virulence of Francisella tularensis
Degabriel, Virulence 2023 - “...155 , 156 ]. Indeed, F. novicida uses the CRISPR/cas9 system to regulate lipoprotein expression (FTN_1103) and evades TLR2 recognition. Interestingly, Cas9 and the associated tracrRNA and scaRNA are induced by a 100 fold during infection, which correlates with the downregulation of FTN_1103 [ 156 ]....”
- CRISPR-dependent endogenous gene regulation is required for virulence in piscine Streptococcus agalactiae
Dong, Emerging microbes & infections 2021 - “...on F. novicida have shown that CRISPR-Cas components could downregulate the expression of the lipoprotein FTN_1103 by promoting its mRNA degradation and therefore facilitate bacterial immune evasion [ 8 ]. In agreement with this, we found that CRISPR reduced TLR2-dependent expression of the proinflammatory cytokine IL-6...”
- Virulence of the Pathogen Porphyromonas gingivalis Is Controlled by the CRISPR-Cas Protein Cas3
Solbiati, mSystems 2020 - “...the innate immune response by regulating levels of the endogenous transcript of bacterial lipoprotein (BLP; FTN_1103) via a CRISPR/Cas-associated RNA (small Cajal body-specific RNA [scaRNA]) ( 7 ), and P. aeruginosa evades host defenses by targeting mRNA of the quorum-sensing regulator LasR to dampen the recognition...”
- Catalytically Active Cas9 Mediates Transcriptional Interference to Facilitate Bacterial Virulence
Ratner, Molecular cell 2019 - “...Cas9 (FnoCas9) regulation of the expression of an endogenous mRNA encoding a bacterial lipoprotein (BLP), FTN_1103 (1103) ( Jones et al., 2012 ). Regulation of 1103 by FnoCas9 is controlled by tracrRNA and scaRNA, which is a distinct small RNA transcribed from an independent promoter near...”
- “...1,782 genes (0.22%) were significantly up-regulated in the cas9 mutant by more than 2-fold, including FTN_1103 (1103) encoding a bacterial lipoprotein (BLP), which we previously demonstrated to be regulated by FnoCas9 ( Figure 1A , S1A , C - F ) ( Jones et al., 2012...”
- CRISPR-Cas Targeting of Host Genes as an Antiviral Strategy
Chen, Viruses 2018 - “...typical CRISPR/Cas systems that induce foreign DNA breakage and degradation, FnCas9 modulates endogenous anti-bacterial lipoprotein FTN_1103 (BLP) expression by degrading its mRNA, thus facilitating bacterial evasion of this innate immune defense system and increasing virulence [ 37 ]. Because FnCas9, tracrRNA and scaRNA contribute to virulence,...”
- A response regulator promotes Francisella tularensis intramacrophage growth by repressing an anti-virulence factor
Ramsey, Molecular microbiology 2016 - “...novicida , Cas9 of the CRISPR-Cas system promotes virulence by inhibiting the expression of the FTN_1103 gene encoding a lipoprotein ( Sampson et al. , 2013 ). In this situation, FTN_1103 functions as an anti-virulence factor not by inhibiting intramacrophage growth ( Weiss et al. ,...”
- Current and future prospects for CRISPR-based tools in bacteria
Luo, Biotechnology and bioengineering 2016 - “...demonstrated that the Type II-B Cas9 from Francisella novicida naturally represses the expression of the FTN_1103 bacterial lipoprotein as part of immune avoidance during host infection. Silencing required the tracrRNA as well as a small, CRISPR-Cas-associated RNA (scaRNA) encoded adjacent to the CRISPR array. The scaRNA...”
- Enterococcus faecalis Glycolipids Modulate Lipoprotein-Content of the Bacterial Cell Membrane and Host Immune Response
Theilacker, PloS one 2015 - “...46 ]. The F . novicida protein FTN_0757 specifically represses the production of its lipoprotein FTN_1103 and deletion of FTN_0757 leads to stronger stimulation of TLR2 and induction of higher levels of inflammatory cytokines compared to wild-type bacteria [ 46 ]. Hence, FTN_0757 was suggested to...”
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FTN_1101 hypothetical protein from Francisella tularensis subsp. novicida U112
Aligns to 5:274 / 277 (97.5%), covers 100.0% of PF11685, 280.1 bits
- Catalytically Active Cas9 Mediates Transcriptional Interference to Facilitate Bacterial Virulence
Ratner, Molecular cell 2019 - “...revealed the identical regulon of only four genes (1103; FTN_1104 , 1104; FTN_1102 , 1102; FTN_1101 , 1101) ( Figure 1A , S1A ). These results, validated by Northern blots of 1104-1101 in cas9 , tracrRNA, scaRNA, crRNA, and the complemented strains, indicated that similar to...”
- Degeneration of a CRISPR/Cas system and its regulatory target during the evolution of a pathogen
Sampson, RNA biology 2013 - “...the loss of the ~2 kbp region that contains FTN_1101, FTN_1102, and FTN_1103 in the F. novicida genome. The FTN_1103 region within F. novicida is flanked by...”
- Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis
Horzempa, BMC microbiology 2008 - “...in its genome, suggesting the possibility of functional redundancy. These alternate forms (FTN_1103, 52% identity; FTN_1101, 49% identity) may have compensated for a mutation of tivA (FTN_0573) during the negative-selection screen [ 14 ], thereby masking any effects on pathogenesis. This possibility underscores the value of...”
FTN_1104 hypothetical protein from Francisella tularensis subsp. novicida U112
Aligns to 9:173 / 182 (90.7%), covers 56.6% of PF11685, 211.4 bits
- Catalytically Active Cas9 Mediates Transcriptional Interference to Facilitate Bacterial Virulence
Ratner, Molecular cell 2019 - “...in tracrRNA and scaRNA deletion strains revealed the identical regulon of only four genes (1103; FTN_1104 , 1104; FTN_1102 , 1102; FTN_1101 , 1101) ( Figure 1A , S1A ). These results, validated by Northern blots of 1104-1101 in cas9 , tracrRNA, scaRNA, crRNA, and the...”
- Degeneration of a CRISPR/Cas system and its regulatory target during the evolution of a pathogen
Sampson, RNA biology 2013 - “...F. tularensis genome. FTN_1102 is also absent, and the FTN_1104 ortholog (FTT1122c) is truncated by 78 bases (Fig. 2). There is evidence of a transposon...”
FTL_0841 hypothetical lipoprotein from Francisella tularensis subsp. holarctica
Aligns to 1:142 / 143 (99.3%), covers 52.4% of PF11685, 137.3 bits
FTL_0840 hypothetical protein from Francisella tularensis subsp. holarctica
Aligns to 9:112 / 113 (92.0%), covers 35.2% of PF11685, 122.7 bits
Or search for genetic data about PF11685 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory