Family Search for PF12097 (DUF3573)
April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.
PF12097 hits 16 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
FTT_0918 hypothetical protein from Francisella tularensis subsp. tularensis SCHU S4
Aligns to 4:422 / 557 (75.2%), covers 99.5% of PF12097, 689.6 bits
- Diverse roles of low-molecular weight thiol GSH in Francisella's virulence, location sensing and GSH-stealing from host
van, Current research in microbial sciences 2024 - “...reported ( Ireland etal., 2019 ) fupA $ GSH porin ( Wangetal., 2023 ). FTN_0444; FTT_0918; FTL_0439. 0.6 2.05 Up Regulated at 4h (1.97) but Down at 8h (0.8). Essential ( Ramseyetal., 2020 ) (1.55 log2FC). Essential in U937 cells ( Asareand Abu Kwaik,2011 ) Essential...”
- Genetic Determinants of Antibiotic Resistance in Francisella
Kassinger, Frontiers in microbiology 2021 - “...the quinolone nalidixic acid, supporting this hypothesis. It was found that deletion of FupA/B gene (FTT_0918), which encodes an iron-binding membrane protein, also contributed to fluoroquinolone resistance. While the exact mechanism is unclear, the authors suggest that deletion of FupA/B destabilizes the cell membrane leading to...”
- Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Siebert, Emerging microbes & infections 2019 - “...gene which results from a fusion associated with a recombinational deletion event between adjacent fupA (FTT_0918) and fupB (FTT_0919) genes, two paralogs present in F. tularensis subsp. holarctica FSC200 and close relatives, including F. tularensis subsp. tularensis SCHU S4 [ 10 ]. Together with FTT_0025c (...”
- Protection induced by a Francisella tularensis subunit vaccine delivered by glucan particles
Whelan, PloS one 2018 - “...likely to elicit an immunosuppressive response [ 36 , 38 ]. These proteins were 1740 (FTT_0918), Tep3 (FTT_1754), KatG (FTT_0721) for MHC I, and 1740 (FTT_0918), Tep3 (FTT_1754), 2227 (FTT_1768) and Tep15 (FTT_0540) for MHC II. These proteins are each in the top 10% within the...”
- Comparative Transcriptional Analyses of Francisella tularensis and Francisella novicida
Sarva, PloS one 2016 - “...uptake of ferrous iron [ 60 ]. The ortholog of FTH_0431 in Ftt Schu S4 (FTT_0918) was shown to be required for virulence and a strain with a mutation in this gene was effective as a live vaccine [ 61 ]. Reintroduction of the FTH_0431 (...”
- Identification of Genome-Wide Mutations in Ciprofloxacin-Resistant F. tularensis LVS Using Whole Genome Tiling Arrays and Next Generation Sequencing
Jaing, PloS one 2016 (no snippet) - FmvB: A Francisella tularensis Magnesium-Responsive Outer Membrane Protein that Plays a Role in Virulence
Wu, PloS one 2016 - “...kanamycin (kan). Sequence comparisons and bioinformatics predictions Amino acid sequence comparisons of FslE (FTT_0025c), FupA (FTT_0918), FupB (FTT_0919), FmvA (FTT_0267), and FmvB (FTT_0602c) were performed using BLASTP analysis ( http://blast.ncbi.nlm.nih.gov ) and ClustalW alignment (MacVector version 12.6.0). Bacterial protein localization was predicted by PSORTb version 3.0.2...”
- Role of pathogenicity determinant protein C (PdpC) in determining the virulence of the Francisella tularensis subspecies tularensis SCHU
Uda, PloS one 2014 - “...for metalion transporter proteins (locus_tag; FTT_0615c), pdpC1 (FTT_1354), pdpC2 (FTT_1709), and RD18 (intergenic region from FTT_0918 to FTT_0919) of the attenuated F. tularensis FSC043. Because only pdpC was mutated in both attenuated strains, SCHU P0 and FSC043, it is possible that pdpC alone determined the virulence...”
- More
FTN_0444 membrane protein of unknown function from Francisella tularensis subsp. novicida U112
Aligns to 4:422 / 557 (75.2%), covers 99.5% of PF12097, 686.2 bits
- Diverse roles of low-molecular weight thiol GSH in Francisella's virulence, location sensing and GSH-stealing from host
van, Current research in microbial sciences 2024 - “...effect reported ( Ireland etal., 2019 ) fupA $ GSH porin ( Wangetal., 2023 ). FTN_0444; FTT_0918; FTL_0439. 0.6 2.05 Up Regulated at 4h (1.97) but Down at 8h (0.8). Essential ( Ramseyetal., 2020 ) (1.55 log2FC). Essential in U937 cells ( Asareand Abu Kwaik,2011 )...”
- “...transporter in F. novicida Recent studies by Wangetal.(2023) have identified the outer membrane porin FupA (FTN_0444) as the import porin for intact GSH or Cys-Gly dipeptide for Francisella . This could provide a mechanism of GSH import (aka stealing) from host cytosol or other GSH-rich environments....”
- Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Siebert, Emerging microbes & infections 2019 - “...(U112_Fno3) bearing a functionally-inert DNA gyrase mutation (GyrA_E524, S525) also had a nonsense mutation in FTN_0444 ( fupA ) [ 7 ]. Moreover, fupA was identified as one of seven mutated genes in a ciprofloxacin-resistant mutant of F. tularensis SCHU S4, alongside gyrA and parE [...”
- “...the presence of a greater abundance of proteins in the culture supernatant with a fupA (FTN_0444) strain compared to the wild-type [ 26 ]. In agreement with these observations with F. novicida U112 , a comparative proteomics analysis of LVS and LVS fupA/B secretomes revealed strong...”
- Evasion of IFN-γ signaling by Francisella novicida is dependent upon Francisella outer membrane protein C
Nallaparaju, PloS one 2011 - “...as a virulence factor. We generated a F. novicida ( F. tularensis subsp. novicida ) FTN_0444 (homolog of FTT0918) fopC mutant to study the virulence-associated mechanism(s) of FTT0918. Methods and Findings The fopC strain phenotype was characterized using immunological and biochemical assays. Attenuated virulence via the...”
- “...lipoprotein (designated FopC) in F. tularensis virulence and pathogenicity, we generated a defined F. novicida FTN_0444 (homolog to FTT0918, 99% identity in aa sequence) mutant, designated KKF332, and characterized the contribution of this protein to the virulence of this microorganism. Our results demonstrate that FopC plays...”
- TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu
Sharma, Cellular immunology 2011 - “...type F. novicida strain U112 and the transposon mutants lacking the 58kDa protein (locus tag FTN_0444) were kindly provided by Dr. L. Gallagher [ 8 ]. The Live Vaccine Strain (LVS) and Francisella tularensis Type A strain SchuS4 was kindly provided by Dr. Karl E. Klose...”
- Common ancestry and novel genetic traits of Francisella novicida-like isolates from North America and Australia as revealed by comparative genomic analyses
Siddaramappa, Applied and environmental microbiology 2011 - “...strains 3523, Fx1, and U112 (FN3523_0408, FNFX1_0437, and FTN_0444, respectively). The presence of FPI, fur, fslABCDEF, and fupA in almost all Francisella...”
- Molecular complexity orchestrates modulation of phagosome biogenesis and escape to the cytosol of macrophages by Francisella tularensis
Asare, Environmental microbiology 2010 - “...unknown function 3 2 # tnfn1_pw060420p04q134 FTN_0297 conserved protein of unknown function 7 7 tnfn1_pw060328p05q119 FTN_0444 membrane protein of unknown function 6 6 # tnfn1_pw060420p03q175 FTN_0444 membrane protein of unknown function 5 5 # tnfn1_pw060323p07q141 FTN_0788 conserved protein of unknown function 5 5 tnfn1_pw060420p04q176 FTN_0855 protein...”
- “...unknown function tnfn1_pw060323p06q113 FTN_0420 purCD SAICAR synthetase/phosphoribosylamine-glycine ligase tnfn1_pw060328p06q134 FTN_0443 maeA NAD-dependent malic enzyme tnfn1_pw060328p05q119 FTN_0444 membrane protein of unknown function tnfn1_pw060323p05q182 FTN_0504 cadC lysine decarboxylase tnfn1_pw060510p01q124 FTN_0507 gcvP1 glycine cleavage system P protein, subunit 1 tnfn1_pw060323p06q168 FTN_0545 glycosyl transferase, group 2 tnfn1_pw060419p02q135 FTN_0560 ksgA dimethyladenosine...”
- Exploitation of host cell biology and evasion of immunity by francisella tularensis
Asare, Frontiers in microbiology 2010 - “...Protein of unknown function FTN_0297 Conserved protein of unknown function FTN_0428 Protein of unknown function FTN_0444 Membrane protein of unknown function FTN_0477 Conserved protein of unknown function FTN_0788 Conserved protein of unknown function FTN_0855 Protein of unknown function FTN_0915 Conserved protein of unknown function FTN_0925 Protein...”
- “...of unknown function FTN_0149 Conserved protein of unknown function FTN_0297 Conserved protein of unknown function FTN_0444 Membrane protein of unknown function FTN_0788 Conserved protein of unknown function FTN_0855 Protein of unknown function FTN_0915 Conserved protein of unknown function FTN_0925 Protein of unknown function FTN_0930 Protein of...”
- Molecular bases of proliferation of Francisella tularensis in arthropod vectors
Asare, Environmental microbiology 2010 - “...unknown function 3 2 # tnfn1_pw060420p04q134 FTN_0297 conserved protein of unknown function 7 7 tnfn1_pw060328p05q119 FTN_0444 membrane protein of unknown function 6 6 # tnfn1_pw060420p03q175 FTN_0444 membrane protein of unknown function 5 5 # tnfn1_pw060323p07q141 FTN_0788 conserved protein of unknown function 5 5 tnfn1_pw060420p04q176 FTN_0855 protein...”
- “...function tnfn1_pw060323p06q113 FTN_0420 purCD SAICAR synthetase/phosphoribosylamine- glycine ligase tnfn1_pw060328p06q134 FTN_0443 maeA NAD-dependent malic enzyme tnfn1_pw060328p05q119 FTN_0444 membrane protein of unknown function tnfn1_pw060323p05q182 FTN_0504 cadC lysine decarboxylase tnfn1_pw060510p01q124 FTN_0507 gcvP1 glycine cleavage system P protein, subunit 1 tnfn1_pw060323p06q168 FTN_0545 glycosyl transferase, group 2 tnfn1_pw060419p02q135 FTN_0560 ksgA dimethyladenosine...”
- More
TC 9.A.51.1.1 / Q5NIP1 The Francisella siderophore transporter, FslE from Francisella tularensis subsp. tularensis (see paper)
FTT_0025c hypothetical protein from Francisella tularensis subsp. tularensis SCHU S4
Aligns to 1:374 / 509 (73.5%), covers 100.0% of PF12097, 684.9 bits
- substrates: Siderophores
- Identification of an Attenuated Substrain of Francisella tularensis SCHU S4 by Phenotypic and Genotypic Analyses
Lovchik, Pathogens (Basel, Switzerland) 2021 - “...(5S ribosomal RNA) NR-643 & FTS-635 24609 A G Intergenic SNP between two hypothetical proteins (FTT_0025c and FTT_0026c) NR-643 & FTS-635 427432 A - Single base deletion causes a frameshift in protein FTT_0415: glgC NR-643 & FTS-635 541270 TTTATATAAGT - 11 bp intergenic deletion between FTT_0517...”
- Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Siebert, Emerging microbes & infections 2019 - “...and close relatives, including F. tularensis subsp. tularensis SCHU S4 [ 10 ]. Together with FTT_0025c ( fslE ), FTT_0267 ( Francisella metal and virulence; fmvA ) [ 11 ], FTT_0602c ( fmvB ) [ 11 ], fupA and fupB, fupA/B belong to a family of...”
- A spontaneous mutation in kdsD, a biosynthesis gene for 3 Deoxy-D-manno-Octulosonic Acid, occurred in a ciprofloxacin resistant strain of Francisella tularensis and caused a high level of attenuation in murine models of tularemia
Chance, PloS one 2017 - “...antigen variable number repeat 2,379 bp CT at 1517 bp. ThrIle. Intergenic region Function Mutation FTT_0025c - FTT_0026c Hypothetical protein & drug resistance transporter, Bcr/CflA subfamily AG glgCglgA Glucose-1-phosphate adenylyltransferase & glycogen synthase Deletion of A FTT_0517 prmA Hypothetical protein & 50S ribosomal protein L11 methyltransferase...”
- FmvB: A Francisella tularensis Magnesium-Responsive Outer Membrane Protein that Plays a Role in Virulence
Wu, PloS one 2016 - “...30 mg/L kanamycin (kan). Sequence comparisons and bioinformatics predictions Amino acid sequence comparisons of FslE (FTT_0025c), FupA (FTT_0918), FupB (FTT_0919), FmvA (FTT_0267), and FmvB (FTT_0602c) were performed using BLASTP analysis ( http://blast.ncbi.nlm.nih.gov ) and ClustalW alignment (MacVector version 12.6.0). Bacterial protein localization was predicted by PSORTb...”
FTA_0461 hypothetical lipoprotein from Francisella tularensis subsp. holarctica FTA
Aligns to 3:421 / 556 (75.4%), covers 99.5% of PF12097, 683.3 bits
- Complete genome sequence of Francisella tularensis subspecies holarctica FTNF002-00
Barabote, PloS one 2009 - “...(pseudogene); FTA_0415 (type IV pilus assembly protein) 8 407597409077 (1481 bp) Region deleted in LVS. FTA_0461 (lipoprotein); FTA_0462 (hypothetical protein) 9 484148484319 (172 bp) Region deleted in LVS. FTA_0522 (GMP kinases ATP-binding protein) 10 10411461041356 (211 bp) Region deleted in LVS. FTH_1057 (conserved hypothetical); FTH_1058 (pseudogene);...”
FTL_1836 hypothetical membrane protein from Francisella tularensis subsp. holarctica
Aligns to 1:374 / 509 (73.5%), covers 100.0% of PF12097, 682.2 bits
- Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Siebert, Emerging microbes & infections 2019 - “...protein FupA/B 196.48 5,5226E-05 7,52577196 nd FTL_1931 Hypoxanthine-guanine phosphoribosyl transferase 2.4.2.8 35.762 3,7272E-06 7,95033342 4,970,24 FTL_1836 DUF3573 domain-containing protein 106.53 8,1905E-06 7,66110499 4,451,26 FTL_1835 Bcr/CflA family drug resistance efflux transporter 10.788 4,2138E-05 4,74375095 2,450,50 FTL_1842 Aspartyl/glutamyl-tRNA amidotransferase subunit A 25.759 0,00070266 3,29921922 3,50,37 FTL_0146 ABC transporter,...”
- “...not determined. Strikingly, among the 22 proteins enriched in OMVs purified from the fupA/B mutant, FTL_1836, corresponding to the paralogous FupA protein FslE (Supp Table 1), was one of the proteins with the highest level of overexpression. Strains deleted for fupA/B also produced vesicles with increased...”
- Lack of OxyR and KatG Results in Extreme Susceptibility of Francisella tularensis LVS to Oxidative Stress and Marked Attenuation In vivo
Honn, Frontiers in cellular and infection microbiology 2017 - “...), fslB ( FTL_1833 ), fslC ( FTL_1834 ), fslD ( FTL_1835 ), fslE ( FTL_1836 ), fupA ( FTL_0439 ), furA ( FTL_1831 ), (Lindgren et al., 2009 ), tul4, iglC ( FTL_0113 ), (Brms et al., 2009 ), mglA ( FTL_0260 ), feoB (...”
FTH_0431 hypothetical protein from Francisella tularensis subsp. holarctica OSU18
Aligns to 4:422 / 557 (75.2%), covers 99.5% of PF12097, 680.5 bits
- Comparative Transcriptional Analyses of Francisella tularensis and Francisella novicida
Sarva, PloS one 2016 - “...genome architecture ( Fig 5 , Tables 4 and 5 ). There are two paralogs, FTH_0431 ( fopC ) and FTH_0432, in Fth that share 50.4% homology and that have undergone recombination to form a fusion gene in LVS, FTL_0439. The difference in architecture of the...”
- “...Ftt Schu4, the two genes in the orthologous genomic region have different levels of expression; FTH_0431 ( fopC ) is highly expressed and FTH_0432 has a much lower expression. Due to the fusion of the C-terminal region of FTH_0432 (probe set region D) to the N-terminal...”
FTN_1686 hypothetical membrane protein from Francisella tularensis subsp. novicida U112
Aligns to 1:374 / 509 (73.5%), covers 100.0% of PF12097, 678.4 bits
- Exploitation of host cell biology and evasion of immunity by francisella tularensis
Asare, Frontiers in microbiology 2010 - “...Conserved hypothetical protein FTN_1406 Conserved hypothetical membrane protein FTN_1612 Hypothetical protein FTN_1656 Conserved hypothetical protein FTN_1686 Hypothetical membrane protein FTN_1736 Hypothetical protein FTT1103 Conserved hypothetical lipoprotein FTT1236 Hypothetical protein FTT1244c yfiO Conserved hypothetical lipoprotein FPI PROTEINS FTN_1309 pdpA Protein of unknown function FTN_1310 icmF Conserved protein...”
- “...FTN_1349 Hypothetical protein FTN_1395 Conserved hypothetical protein FTN_1406 Conserved hypothetical membrane protein FTN_1612 Hypothetical protein FTN_1686 Hypothetical membrane protein FTT1103 Conserved hypothetical lipoprotein FPI PROTEINS FTN_1309 pdpA Protein of unknown function FTN_1313 vgrG FTN_1317 iglI Intracellular growth locus, subunit I FTN_1322 iglC Intracellular growth locus, subunit...”
- Molecular complexity orchestrates modulation of phagosome biogenesis and escape to the cytosol of macrophages by Francisella tularensis
Asare, Environmental microbiology 2010 - “...4 tnfn1_pw060328p04q136 FTN_1406 conserved hypothetical membrane protein 4 tnfn1_pw060420p02q127 FTN_1656 conserved hypothetical protein 2 tnfn1_pw060420p02q176 FTN_1686 hypothetical membrane protein 5 tnfn1_pw060418p03q159 FTN_1736 hypothetical protein 2 Metabolic Proteins tnfn1_pw060419p02q150 FTN_0090 acpA acid phosphatase 5 tnfn1_pw060419p03q169 FTN_0218 nfnB dihydropteridine reductase 2 tnfn1_pw060420p01q123 FTN_0524 asd aspartate semialdehyde dehydrogenase 5...”
- “...56 Phagosomal tnfn1_pw060328p06q182 FTN_1395 80 Phagosomal tnfn1_pw060328p04q136 FTN_1406 84 Phagosomal tnfn1_pw060328p02q129 FTN_1612 69 Phagosomal tnfn1_pw060420p02q176 FTN_1686 89 Phagosomal Metabolic Proteins tnfn1_pw060419p02q150 FTN_0090 52 Phagosomal tnfn1_pw060419p01q106 FTN_0111 ribH 45 Cytoplasmic tnfn1_pw060328p06q174 FTN_0125 ackA 80 Phagosomal tnfn1_pw060323p06q113 FTN_0420 37 Cytoplasmic tnfn1_pw060323p05q182 FTN_0504 67 Phagosomal tnfn1_pw060510p01q124 FTN_0507 gcvP1 28...”
- Molecular bases of proliferation of Francisella tularensis in arthropod vectors
Asare, Environmental microbiology 2010 - “...4 tnfn1_pw060328p04q136 FTN_1406 conserved hypothetical membrane protein 4 tnfn1_pw060420p02q127 FTN_1656 conserved hypothetical protein 2 tnfn1_pw060420p02q176 FTN_1686 hypothetical membrane protein 5 tnfn1_pw060418p03q159 FTN_1736 hypothetical protein 2 Metabolic Proteins tnfn1_pw060419p02q150 FTN_0090 acpA acid phosphatase 5 tnfn1_pw060419p03q169 FTN_0218 nfnB dihydropteridine reductase 2 tnfn1_pw060420p01q123 FTN_0524 asd aspartate semialdehyde dehydrogenase 5...”
- Working toward the future: insights into Francisella tularensis pathogenesis and vaccine development
Pechous, Microbiology and molecular biology reviews : MMBR 2009 - “...FTN_1658 FTN_1659 FTN_1665 FTN_1673 FTN_1682 FTN_1683 FTN_1684 FTN_1686 Method(s)a Function VOL. 73, 2009 VACCINES FOR FRANCISELLA 697 TABLE 3. F. tularensis...”
- Characterization of fig operon mutants of Francisella novicida U112
Kiss, FEMS microbiology letters 2008 - “...FTN_1683 - figB; FTN_1684 - figC; FTN_1685 - figD; FTN_1686 - figE; FTN_1687 - figF. (b) Schematic representation of the five different fig operon mutants used...”
FTT_0602c hypothetical protein from Francisella tularensis subsp. tularensis SCHU S4
Aligns to 10:373 / 492 (74.0%), covers 100.0% of PF12097, 634.2 bits
- Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Siebert, Emerging microbes & infections 2019 - “...FTT_0025c ( fslE ), FTT_0267 ( Francisella metal and virulence; fmvA ) [ 11 ], FTT_0602c ( fmvB ) [ 11 ], fupA and fupB, fupA/B belong to a family of genes encoding outer membrane proteins which are thought to be unique to the Francisella genus...”
- FmvB: A Francisella tularensis Magnesium-Responsive Outer Membrane Protein that Plays a Role in Virulence
Wu, PloS one 2016 - “...Amino acid sequence comparisons of FslE (FTT_0025c), FupA (FTT_0918), FupB (FTT_0919), FmvA (FTT_0267), and FmvB (FTT_0602c) were performed using BLASTP analysis ( http://blast.ncbi.nlm.nih.gov ) and ClustalW alignment (MacVector version 12.6.0). Bacterial protein localization was predicted by PSORTb version 3.0.2 ( http://www.psort.org/psortb/ ). Beta-barrel structural predictions were...”
FTL_0867 hypothetical protein from Francisella tularensis subsp. holarctica
Aligns to 3:366 / 485 (75.1%), covers 100.0% of PF12097, 629.4 bits
FTA_0919 hypothetical protein from Francisella tularensis subsp. holarctica FTA
Aligns to 3:366 / 485 (75.1%), covers 100.0% of PF12097, 626.3 bits
AW21_RS06025, FTL_RS02265 DUF3573 domain-containing protein from Francisella tularensis subsp. holarctica LVS
FTL_0439 hypothetical protein from Francisella tularensis subsp. holarctica
Aligns to 4:415 / 551 (74.8%), covers 94.5% of PF12097, 546.6 bits
- A study of innate immune kinetics reveals a role for a chloride transporter in a virulent Francisella tularensis type B strain
Matz, MicrobiologyOpen 2021 - “...formed by the fusion of two neighboring siderophore genes, fup A , and fup B (FTL_RS02265). The type A fupA mutant showed reduced virulence in an intradermal mouse infection model; however, the LD 50 was 10fold lower than LVS, suggesting LVS is attenuated by additional mechanisms...”
- Evolution of Antibiotic Resistance in Surrogates of Francisella tularensis (LVS and Francisella novicida): Effects on Biofilm Formation and Fitness
Biot, Frontiers in microbiology 2020 - “...A frameshift mutation was also identified in the gene encoding for DUF-3573 domain-containing protein FupA (AW21_RS06025). A single mutation was identified in the gene encoding for IS630 family transposase, and the remaining mutations were located in intergenic regions, classified as modifiers in this study, due to...”
- “...assembly factor BamB 870delT Asp290fs High Intergenic region AW21_RS03145-AW21_RS03150 N/A 599763C > A Modifier Synonymous AW21_RS06025 DUF3573 domain-containing protein FupA 105G > C Gly35Gly Low Frameshift AW21_RS06025 DUF3573 domain-containing protein FupA 107dupC Leu37fs High Missense AW21_RS06205 Transglycosylase SLT domain protein 1868A > T Lys623Ile Moderate Deletion...”
- Diverse roles of low-molecular weight thiol GSH in Francisella's virulence, location sensing and GSH-stealing from host
van, Current research in microbial sciences 2024 - “...( Ireland etal., 2019 ) fupA $ GSH porin ( Wangetal., 2023 ). FTN_0444; FTT_0918; FTL_0439. 0.6 2.05 Up Regulated at 4h (1.97) but Down at 8h (0.8). Essential ( Ramseyetal., 2020 ) (1.55 log2FC). Essential in U937 cells ( Asareand Abu Kwaik,2011 ) Essential in...”
- Genomic trajectories to fluoroquinolone resistance in Francisella tularensis subsp. holarctica live vaccine strain
Sutera, International journal of antimicrobial agents 2020 (PubMed)- “...J774 cells. In contrast, all mutations at locus FTL_0439 encoding FupA/B, a membrane protein involved in iron transport, were associated with FQ resistance and...”
- “...FTL_0533 gyrA FTL_1547 gyrB FTL_0462 parC FTL_1726 parE FTL_0917 Trcf FTL_0439 FupA/B P0 1 0.016 P1 10 0.064 1401g>t E467D P3 1 0.064 + P4 1 4 248c>t T83I...”
- Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Siebert, Emerging microbes & infections 2019 - “...third-round ciprofloxacin-resistant isolates had at least one single - nucleotide polymorphism (SNP) in the gene FTL_0439, which codes for FupA/B (Fer-Utilization Protein). We therefore hypothesized a role for FupA/B in the resistance of F. tularensis LVS against FQ. This hypothesis was supported by whole-genome sequencing of...”
- “...P values are reported in figure legends. Experimental details concerning bioinformatics analysis, construction of the FTL_0439 knock-out strain and the FupA/B complementation plasmid, production of anti-FupA antibody, MIC and iron measurements, western-blots, Dynamic Light Scattering, Mass spectrometry-based quantitative proteomic analyses, Quantitative Real-Time PCR, colony forming unit...”
- Bacterial lipoproteins and other factors released by Francisella tularensis modulate human neutrophil lifespan: Effects of a TLR1 SNP on apoptosis inhibition
Kinkead, Cellular microbiology 2018 - “...Pal in our BLP preparations, and other BLPs detected by silver stain likely include YapH (FTL_0439) and Flpp3 (FTL_0645), FipB, DsbA (FTT1103), and a F.tularensis BLP with homology to Shigella VacJ (FTL_1637; Huntley et al., 2007 ; Parra et al., 2010 ; Straskova et al., 2009...”
- Functional Characterization of the DNA Gyrases in Fluoroquinolone-Resistant Mutants of Francisella novicida
Caspar, Antimicrobial agents and chemotherapy 2017 - “...A, Ramakrishnan G. 2010. The fslE homolog, FTL_0439 (fupA/B), mediates siderophore-dependent iron uptake in Francisella tularensis LVS. Infect Immun...”
- Lack of OxyR and KatG Results in Extreme Susceptibility of Francisella tularensis LVS to Oxidative Stress and Marked Attenuation In vivo
Honn, Frontiers in cellular and infection microbiology 2017 - “...), fslC ( FTL_1834 ), fslD ( FTL_1835 ), fslE ( FTL_1836 ), fupA ( FTL_0439 ), furA ( FTL_1831 ), (Lindgren et al., 2009 ), tul4, iglC ( FTL_0113 ), (Brms et al., 2009 ), mglA ( FTL_0260 ), feoB ( FTL_0133 ), and katG...”
- Identification of Genome-Wide Mutations in Ciprofloxacin-Resistant F. tularensis LVS Using Whole Genome Tiling Arrays and Next Generation Sequencing
Jaing, PloS one 2016 (no snippet) - Comparative Transcriptional Analyses of Francisella tularensis and Francisella novicida
Sarva, PloS one 2016 - “...share 50.4% homology and that have undergone recombination to form a fusion gene in LVS, FTL_0439. The difference in architecture of the gene between the two Fth strains is shown in Fig 5 . In Fth OR960246 and in Ftt Schu4, the two genes in the...”
- More
FTN_0142 hypothetical protein from Francisella tularensis subsp. novicida U112
Aligns to 3:378 / 513 (73.3%), covers 91.6% of PF12097, 394.3 bits
FTN_0445 hypothetical protein from Francisella tularensis subsp. novicida U112
Aligns to 98:340 / 481 (50.5%), covers 60.1% of PF12097, 289.4 bits
FTT_0919 hypothetical protein from Francisella tularensis subsp. tularensis SCHU S4
Aligns to 98:340 / 481 (50.5%), covers 60.1% of PF12097, 282.8 bits
- Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Siebert, Emerging microbes & infections 2019 - “...from a fusion associated with a recombinational deletion event between adjacent fupA (FTT_0918) and fupB (FTT_0919) genes, two paralogs present in F. tularensis subsp. holarctica FSC200 and close relatives, including F. tularensis subsp. tularensis SCHU S4 [ 10 ]. Together with FTT_0025c ( fslE ), FTT_0267...”
- Identification of Genome-Wide Mutations in Ciprofloxacin-Resistant F. tularensis LVS Using Whole Genome Tiling Arrays and Next Generation Sequencing
Jaing, PloS one 2016 (no snippet) - FmvB: A Francisella tularensis Magnesium-Responsive Outer Membrane Protein that Plays a Role in Virulence
Wu, PloS one 2016 - “...Sequence comparisons and bioinformatics predictions Amino acid sequence comparisons of FslE (FTT_0025c), FupA (FTT_0918), FupB (FTT_0919), FmvA (FTT_0267), and FmvB (FTT_0602c) were performed using BLASTP analysis ( http://blast.ncbi.nlm.nih.gov ) and ClustalW alignment (MacVector version 12.6.0). Bacterial protein localization was predicted by PSORTb version 3.0.2 ( http://www.psort.org/psortb/...”
- Role of pathogenicity determinant protein C (PdpC) in determining the virulence of the Francisella tularensis subspecies tularensis SCHU
Uda, PloS one 2014 - “...transporter proteins (locus_tag; FTT_0615c), pdpC1 (FTT_1354), pdpC2 (FTT_1709), and RD18 (intergenic region from FTT_0918 to FTT_0919) of the attenuated F. tularensis FSC043. Because only pdpC was mutated in both attenuated strains, SCHU P0 and FSC043, it is possible that pdpC alone determined the virulence of each...”
- The francisella tularensis proteome and its recognition by antibodies
Kilmury, Frontiers in microbiology 2010 - “...comprising the N-terminal half of the hypothetical protein FTT_0918 and the C-terminal half of hypothetical FTT_0919 as a single protein (Twine et al., 2005a ). A similar defect, resulting in the expression of a homologous protein in LVS was also observed (Twine et al., 2005a ),...”
- “...Huntley et al. ( 2007 ) FTT_0918 Hypothetical protein Huntley et al. ( 2007 ) FTT_0919 Hypothetical protein Huntley et al. ( 2007 ) FTL_0439 YapH-LVS Huntley et al. ( 2007 ) FTT_0729 ABC transporter, membrane protein Janovska et al. ( 2007a ) Putative outer membrane...”
FTT_0267 hypothetical protein from Francisella tularensis subsp. tularensis SCHU S4
Aligns to 16:237 / 372 (59.7%), covers 56.4% of PF12097, 282.3 bits
- Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Siebert, Emerging microbes & infections 2019 - “...F. tularensis subsp. tularensis SCHU S4 [ 10 ]. Together with FTT_0025c ( fslE ), FTT_0267 ( Francisella metal and virulence; fmvA ) [ 11 ], FTT_0602c ( fmvB ) [ 11 ], fupA and fupB, fupA/B belong to a family of genes encoding outer membrane...”
- FmvB: A Francisella tularensis Magnesium-Responsive Outer Membrane Protein that Plays a Role in Virulence
Wu, PloS one 2016 - “...and bioinformatics predictions Amino acid sequence comparisons of FslE (FTT_0025c), FupA (FTT_0918), FupB (FTT_0919), FmvA (FTT_0267), and FmvB (FTT_0602c) were performed using BLASTP analysis ( http://blast.ncbi.nlm.nih.gov ) and ClustalW alignment (MacVector version 12.6.0). Bacterial protein localization was predicted by PSORTb version 3.0.2 ( http://www.psort.org/psortb/ ). Beta-barrel...”
- The fslE homolog, FTL_0439 (fupA/B), mediates siderophore-dependent iron uptake in Francisella tularensis LVS
Sen, Infection and immunity 2010 - “...amino terminus relative to the Schu S4 gene FTT_0267 (Fig. 3A). In order to determine the functionality of these LVS-specific paralogs in siderophore...”
FTH_0432 hypothetical protein from Francisella tularensis subsp. holarctica OSU18
FTA_0462 hypothetical protein from Francisella tularensis subsp. holarctica FTA
Aligns to 101:344 / 481 (50.7%), covers 60.1% of PF12097, 282.1 bits
- Comparative Transcriptional Analyses of Francisella tularensis and Francisella novicida
Sarva, PloS one 2016 - “..., Tables 4 and 5 ). There are two paralogs, FTH_0431 ( fopC ) and FTH_0432, in Fth that share 50.4% homology and that have undergone recombination to form a fusion gene in LVS, FTL_0439. The difference in architecture of the gene between the two Fth...”
- “...genomic region have different levels of expression; FTH_0431 ( fopC ) is highly expressed and FTH_0432 has a much lower expression. Due to the fusion of the C-terminal region of FTH_0432 (probe set region D) to the N-terminal region of FTH_0431 (probe set region A) in...”
- Complete genome sequence of Francisella tularensis subspecies holarctica FTNF002-00
Barabote, PloS one 2009 - “...(type IV pilus assembly protein) 8 407597409077 (1481 bp) Region deleted in LVS. FTA_0461 (lipoprotein); FTA_0462 (hypothetical protein) 9 484148484319 (172 bp) Region deleted in LVS. FTA_0522 (GMP kinases ATP-binding protein) 10 10411461041356 (211 bp) Region deleted in LVS. FTH_1057 (conserved hypothetical); FTH_1058 (pseudogene); FTH_1059 (pseudogene)...”
Or search for genetic data about PF12097 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory