Family Search for PF12130 (bMERB_dom)
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Running HMMer for PF12130
PF12130 hits 50 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.
MILK1_BOVIN / E1BBG2 MICAL-like protein 1 from Bos taurus (Bovine) (see 3 papers)
Aligns to 674:806 / 853 (15.6%), covers 99.3% of PF12130, 151.5 bits
- function: Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth (By similarity).
subunit: Homooligomer (Probable). Interacts (via NPF1 motif) with EHD1 (via EH domain); the interaction is direct and probably recruits EHD1 to membranes. Interacts with EHD3 (via EH domain). Interacts with RAB35 (GTP-bound form); the interaction is direct and probably recruits MICALL1 to membranes. Interacts with ACAP2; the interaction is indirect through RAB35. Interacts with RAB8A (GTP-bound form); regulates RAB8A association with recycling endosomes. Interacts with RAB13 (GTP-bound form). Interacts with ARF6 (GTP-bound form). Interacts with PACSIN2 (via the SH3 domain). Interacts with DPYSL2.
MILK1_MOUSE / Q8BGT6 MICAL-like protein 1; Molecule interacting with Rab13; MIRab13 from Mus musculus (Mouse) (see 2 papers)
Aligns to 691:823 / 870 (15.3%), covers 98.5% of PF12130, 151.3 bits
- function: Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth.
subunit: Homooligomer (Probable). Interacts (via NPF1 motif) with EHD1 (via EH domain); the interaction is direct and probably recruits EHD1 to membranes. Interacts with EHD3 (via EH domain). Interacts with RAB35 (GTP-bound form); the interaction is direct and probably recruits MICALL1 to membranes. Interacts with ACAP2; the interaction is indirect through RAB35. Interacts with RAB8A (GTP-bound form); regulates RAB8A association with recycling endosomes. Interacts with RAB13 (GTP-bound form). Interacts with ARF6 (GTP-bound form). Interacts with PACSIN2 (via the SH3 domain). Interacts with DPYSL2.
MILK1_RAT / D3ZQL6 MICAL-like protein 1 from Rattus norvegicus (Rat) (see paper)
Aligns to 676:808 / 855 (15.6%), covers 98.5% of PF12130, 151.1 bits
- function: Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth.
subunit: Homooligomer (Probable). Interacts (via NPF1 motif) with EHD1 (via EH domain); the interaction is direct and probably recruits EHD1 to membranes. Interacts with EHD3 (via EH domain). Interacts with RAB35 (GTP-bound form); the interaction is direct and probably recruits MICALL1 to membranes. Interacts with ACAP2; the interaction is indirect through RAB35. Interacts with RAB8A (GTP-bound form); regulates RAB8A association with recycling endosomes. Interacts with RAB13 (GTP-bound form). Interacts with ARF6 (GTP-bound form). Interacts with PACSIN2 (via the SH3 domain). Interacts with DPYSL2.
MILK1_HUMAN / Q8N3F8 MICAL-like protein 1; Molecule interacting with Rab13; MIRab13 from Homo sapiens (Human) (see 6 papers)
Aligns to 684:816 / 863 (15.4%), covers 99.3% of PF12130, 151.0 bits
- function: Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth.
subunit: Homooligomer (Probable). Interacts (via NPF1 motif) with EHD1 (via EH domain); the interaction is direct and probably recruits EHD1 to membranes. Interacts with EHD3 (via EH domain). Interacts with RAB35 (GTP-bound form); the interaction is direct and probably recruits MICALL1 to membranes. Interacts with ACAP2; the interaction is indirect through RAB35. Interacts with RAB8A (GTP-bound form); regulates RAB8A association with recycling endosomes. Interacts with RAB13 (GTP-bound form). Interacts with ARF6 (GTP-bound form). Interacts with PACSIN2 (via the SH3 domain). Interacts with DPYSL2.
MICA3_HUMAN / Q7RTP6 [F-actin]-monooxygenase MICAL3; Molecule interacting with CasL protein 3; MICAL-3; EC 1.14.13.225 from Homo sapiens (Human) (see 6 papers)
Aligns to 1854:1988 / 2002 (6.7%), covers 100.0% of PF12130, 148.6 bits
- function: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Seems to act as Rab effector protein and plays a role in vesicle trafficking. Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process and implicates RAB8A associated with exocytotic vesicles. Required for cytokinesis. Contributes to stabilization and/or maturation of the intercellular bridge independently of its monooxygenase activity. Promotes recruitment of Rab8 and ERC1 to the intercellular bridge, and together these proteins are proposed to function in timely abscission.
catalytic activity: H(+) + L-methionyl-[F-actin] + NADPH + O2 = H2O + L-methionyl- (R)-S-oxide-[F-actin] + NADP(+) (RHEA:51308)
cofactor: FAD
subunit: Interacts with RAB1B, RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); binding to RAB1B is of low affinity compared to other Rab proteins; at least in case of RAB8A can bind 2 molecules of RAB8A simultaneously through a high and a low affinity binding site, respectively. Interacts with ERC1 and RAB8A; may bridge ERC1 with RAB8A. Interacts with KIF23 and ERC1; enhances the interaction between KIF23 and ERC1. Interacts with NINL isoform 2.
MILK2_RAT / D3ZEN0 MICAL-like protein 2; Junctional Rab13-binding protein; Molecule interacting with CasL-like 2; MICAL-L2 from Rattus norvegicus (Rat) (see paper)
Aligns to 851:983 / 1014 (13.1%), covers 98.5% of PF12130, 143.6 bits
- function: Effector of small Rab GTPases which is involved in junctional complexes assembly through the regulation of cell adhesion molecules transport to the plasma membrane and actin cytoskeleton reorganization. Regulates the endocytic recycling of occludins, claudins and E-cadherin to the plasma membrane and may thereby regulate the establishment of tight junctions and adherens junctions. In parallel, may regulate actin cytoskeleton reorganization directly through interaction with F-actin or indirectly through actinins and filamins. Most probably involved in the processes of epithelial cell differentiation, cell spreading and neurite outgrowth.
subunit: Interacts with RAB13 (GTP-bound form); competes with RAB8A and is involved in tight junctions assembly. Interacts with RAB8A; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts with RAB8B. Interacts (preferentially in opened conformation) with ACTN1 and ACTN4; stimulated by RAB13 activation. Interacts (via calponin-homology (CH) domain) with the filamins FLNA, FLNB and FLNC (via actin-binding domain) (By similarity).
MILK2_MOUSE / Q3TN34 MICAL-like protein 2; Junctional Rab13-binding protein; Molecule interacting with CasL-like 2; MICAL-L2 from Mus musculus (Mouse) (see 6 papers)
Aligns to 846:978 / 1009 (13.2%), covers 98.5% of PF12130, 142.3 bits
- function: Effector of small Rab GTPases which is involved in junctional complexes assembly through the regulation of cell adhesion molecules transport to the plasma membrane and actin cytoskeleton reorganization. Regulates the endocytic recycling of occludins, claudins and E-cadherin to the plasma membrane and may thereby regulate the establishment of tight junctions and adherens junctions. In parallel, may regulate actin cytoskeleton reorganization directly through interaction with F-actin or indirectly through actinins and filamins. Most probably involved in the processes of epithelial cell differentiation, cell spreading and neurite outgrowth.
subunit: Interacts with RAB13 (GTP-bound form); competes with RAB8A and is involved in tight junctions assembly. Interacts with RAB8A; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts with RAB8B. Interacts (preferentially in opened conformation) with ACTN1 and ACTN4; stimulated by RAB13 activation. Interacts (via calponin-homology (CH) domain) with the filamins FLNA, FLNB and FLNC (via actin-binding domain).
MILK2_HUMAN / Q8IY33 MICAL-like protein 2; Junctional Rab13-binding protein; Molecule interacting with CasL-like 2; MICAL-L2 from Homo sapiens (Human) (see 2 papers)
Aligns to 740:872 / 904 (14.7%), covers 99.3% of PF12130, 139.4 bits
- function: Effector of small Rab GTPases which is involved in junctional complexes assembly through the regulation of cell adhesion molecules transport to the plasma membrane and actin cytoskeleton reorganization. Regulates the endocytic recycling of occludins, claudins and E-cadherin to the plasma membrane and may thereby regulate the establishment of tight junctions and adherens junctions. In parallel, may regulate actin cytoskeleton reorganization directly through interaction with F-actin or indirectly through actinins and filamins. Most probably involved in the processes of epithelial cell differentiation, cell spreading and neurite outgrowth (By similarity).
subunit: Interacts with RAB13 (GTP-bound form); competes with RAB8A and is involved in tight junctions assembly. Interacts with RAB8A; competes with RAB13 and is involved in E-cadherin endocytic recycling (By similarity). Interacts with RAB8B (By similarity). Interacts (preferentially in opened conformation) with ACTN1 and ACTN4; stimulated by RAB13 activation (By similarity). Interacts (via calponin-homology (CH) domain) with the filamins FLNA, FLNB and FLNC (via actin-binding domain).
MCA2B_DANRE / F1RA39 [F-actin]-monooxygenase mical2b; Molecule interacting with CasL protein 2B; MICAL-2; EC 1.14.13.225 from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
Aligns to 1530:1665 / 1679 (8.1%), covers 99.3% of PF12130, 139.3 bits
- function: Nuclear monooxygenase that promotes depolymerization of F- actin by mediating oxidation of specific methionine residues on actin and regulates the srf signaling. Acts by modifying nuclear actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (By similarity). Acts as a key regulator of the srf signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase mkl1/mrtf-a presence in the nucleus and promote srf:mkl1/mrtf-a- dependent gene transcription (PubMed:24440334).
catalytic activity: H(+) + L-methionyl-[F-actin] + NADPH + O2 = H2O + L-methionyl- (R)-S-oxide-[F-actin] + NADP(+) (RHEA:51308)
cofactor: FAD
disruption phenotype: Small hearts that fail to undergo normal looping at 24 hours post-fertilization (hpf) with thin, linear morphology compared to wild-type hearts at 48 hpf. moreover, cardiomyocytes hearts are spatially disorganized. Defects in srf:mkl1/mrtf-a-dependent gene transcription.
MICA2_HUMAN / O94851 [F-actin]-monooxygenase MICAL2; MICAL C-terminal-like protein; Mical-cL; Molecule interacting with CasL protein 2; MICAL-2; EC 1.14.13.225 from Homo sapiens (Human) (see 5 papers)
Aligns to 1809:1943 / 1957 (6.9%), covers 99.3% of PF12130, 136.8 bits
- function: Methionine monooxygenase that promotes depolymerization of F- actin by mediating oxidation of residues 'Met-44' and 'Met-47' on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:24440334, PubMed:29343822). Regulates the disassembly of branched actin networks also by oxidizing ARP3B-containing ARP2/3 complexes leading to ARP3B dissociation from the network (PubMed:34106209). Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF- A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA (PubMed:24440334).
catalytic activity: H(+) + L-methionyl-[F-actin] + NADPH + O2 = H2O + L-methionyl- (R)-S-oxide-[F-actin] + NADP(+) (RHEA:51308)
cofactor: FAD
subunit: Interacts with PLXNA4 (By similarity). Interacts with RAB1B (PubMed:15694364, PubMed:27552051). Interacts with MAPK1/ERK2 (By similarity). Interacts with RAB35, RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); binding to RAB35 is of low affinity compared to other Rab proteins; at least in case of RAB8A may bind 2 molecules of RAB8A simultaneously through a high and a low affinity binding site, respectively (PubMed:27552051). May interact with MAPK1/ERK2 (By similarity). Interacts with CORO1C; this interaction recruits MICAL2 to the actin filaments (PubMed:34106209).
MICA2_MOUSE / Q8BML1 [F-actin]-monooxygenase MICAL2; ERK2-binding testicular protein 1; MICAL C-terminal-like protein; Mical-cL; Molecule interacting with CasL protein 2; MICAL-2; mMical2; EC 1.14.13.225 from Mus musculus (Mouse) (see 5 papers)
Q8BML1 F-actin monooxygenase (EC 1.14.13.225) from Mus musculus (see paper)
Aligns to 1802:1937 / 1951 (7.0%), covers 99.3% of PF12130, 134.1 bits
- function: Methionine monooxygenase that promotes depolymerization of F- actin by mediating oxidation of residues 'Met-44' and 'Met-47' on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:23911929, PubMed:23927065). Regulates the disassembly of branched actin networks also by oxidizing ARP3B-containing ARP2/3 complexes leading to ARP3B dissociation from the network. Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A- dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA (By similarity).
catalytic activity: H(+) + L-methionyl-[F-actin] + NADPH + O2 = H2O + L-methionyl- (R)-S-oxide-[F-actin] + NADP(+) (RHEA:51308)
cofactor: FAD
subunit: Interacts with PLXNA4 (PubMed:12110185). Interacts with RAB1B (By similarity). Interacts with MAPK1/ERK2 (PubMed:18241670, PubMed:18590835). Interacts with RAB1B, RAB35, RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); binding to RAB1B and RAB35 is of low affinity compared to other Rab proteins; binding to RAB1B and RAB35 is of low affinity compared to other Rab proteins; at least in case of RAB8A may bind 2 molecules of RAB8A simultaneously through a high and a low affinity binding site, respectively (By similarity).
EHBP1_HUMAN / Q8NDI1 EH domain-binding protein 1 from Homo sapiens (Human) (see 3 papers)
Aligns to 1074:1210 / 1231 (11.1%), covers 98.5% of PF12130, 131.7 bits
- function: May play a role in actin reorganization. Links clathrin- mediated endocytosis to the actin cytoskeleton. May act as Rab effector protein and play a role in vesicle trafficking (PubMed:14676205, PubMed:27552051). Required for perinuclear sorting and insulin- regulated recycling of SLC2A4/GLUT4 in adipocytes (By similarity).
subunit: Interacts with EHD1 (By similarity). Interacts with EHD2. Interacts with RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); at least in case of RAB8A may bind 2 molecules of RAB8A simultaneously through a high and a low affinity binding site, respectively.
EHBP1_MOUSE / Q69ZW3 EH domain-binding protein 1 from Mus musculus (Mouse) (see paper)
Aligns to 1074:1210 / 1231 (11.1%), covers 98.5% of PF12130, 130.7 bits
- function: May play a role in actin reorganization. Links clathrin- mediated endocytosis to the actin cytoskeleton. May act as Rab effector protein and play a role in vesicle trafficking (By similarity). Required for perinuclear sorting and insulin-regulated recycling of SLC2A4/GLUT4 in adipocytes.
subunit: Interacts with EHD1 (PubMed:15247266). Interacts with EHD2. Interacts with RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); at least in case of RAB8A may bind 2 molecules of RAB8A simultaneously through a high and a low affinity binding site, respectively (By similarity).
EH1L1_HUMAN / Q8N3D4 EH domain-binding protein 1-like protein 1 from Homo sapiens (Human) (see paper)
Aligns to 1363:1499 / 1523 (9.0%), covers 98.5% of PF12130, 128.9 bits
- function: May act as Rab effector protein and play a role in vesicle trafficking.
subunit: Interacts with RAB8A, RAB10, RAB13 and RAB15 (in their GTP- bound forms); at least in case of RAB8A can bind 2 molecules of RAB8A simultaneously; ternary complex formation of RAB8A, RAB13 and EHBP1L1 is possible.
EH1L1_MOUSE / Q99MS7 EH domain-binding protein 1-like protein 1; Tangerin from Mus musculus (Mouse) (see paper)
Aligns to 1556:1692 / 1716 (8.0%), covers 98.5% of PF12130, 124.4 bits
- function: May act as Rab effector protein and play a role in vesicle trafficking (By similarity). Involved in apical-directed transport in polarized epithelial cells; the functions seems to implicate Rab8, BIN1 and possibly DNM1 (PubMed:26833786).
subunit: Interacts with RAB8A, RAB8B, RAB10, RAB13 and RAB15 (in their GTP-bound forms); at least in case of RAB8A can bind 2 molecules of RAB8A simultaneously. Interacts with BIN1 and AMPH. Interacts with DNM1.
MICA1_HUMAN / Q8TDZ2 [F-actin]-monooxygenase MICAL1; Molecule interacting with CasL protein 1; MICAL-1; NEDD9-interacting protein with calponin homology and LIM domains; EC 1.14.13.225; EC 1.6.3.1 from Homo sapiens (Human) (see 11 papers)
Q8TDZ2 F-actin monooxygenase (EC 1.14.13.225) from Homo sapiens (see 2 papers)
Aligns to 931:1061 / 1067 (12.3%), covers 94.8% of PF12130, 118.0 bits
- function: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:29343822). In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (PubMed:21864500, PubMed:26845023, PubMed:29343822). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels (By similarity). May act as Rab effector protein and play a role in vesicle trafficking. Promotes endosomal tubule extension by associating with RAB8 (RAB8A or RAB8B), RAB10 and GRAF (GRAF1/ARHGAP26 or GRAF2/ARHGAP10) on the endosomal membrane which may connect GRAFs to Rabs, thereby participating in neosynthesized Rab8-Rab10-Rab11-dependent protein export (PubMed:32344433).
catalytic activity: H(+) + L-methionyl-[F-actin] + NADPH + O2 = H2O + L-methionyl- (R)-S-oxide-[F-actin] + NADP(+) (RHEA:51308)
catalytic activity: H(+) + NADPH + O2 = H2O2 + NADP(+) (RHEA:11260)
cofactor: FAD
subunit: Interacts with STK38 and STK38L (By similarity). Interacts with RAB1B, RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); binding to RAB1B is of low affinity compared to other Rab proteins; at least in case of RAB8A and RAB10 can bind 2 molecules of the Rab proteins simultaneously; ternary complex formation of RAB8A, RAB13 and MICAL1 is possible. Associates with the SH3 domain of NEDD9. Interacts with VIM and PLXNA3. Interacts with RAB1B. Interacts with GRAF1/ARHGAP26, GRAF2/ARHGAP10, RAB8A, RAB8B and RAB10; may bind simultaneously to GRAFs and Rabs and connects GRAFs to Rabs (PubMed:32344433). Does not interact with RAB1 and RAB11A (PubMed:32344433).
MICA1_MOUSE / Q8VDP3 [F-actin]-monooxygenase MICAL1; Molecule interacting with CasL protein 1; MICAL-1; mMical1; NEDD9-interacting protein with calponin homology and LIM domains; EC 1.14.13.225; EC 1.6.3.1 from Mus musculus (Mouse) (see 6 papers)
Q8VDP3 F-actin monooxygenase (EC 1.14.13.225) from Mus musculus (see paper)
Aligns to 918:1047 / 1048 (12.4%), covers 95.5% of PF12130, 112.4 bits
- function: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (By similarity). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels. May act as Rab effector protein and play a role in vesicle trafficking. Promotes endosomal tubule extension by associating with RAB8 (RAB8A or RAB8B), RAB10 and GRAF (GRAF1/ARHGAP26 or GRAF2/ARHGAP10) on the endosomal membrane which may connect GRAFs to Rabs, thereby participating in neosynthesized Rab8-Rab10-Rab11-dependent protein export (By similarity).
catalytic activity: H(+) + L-methionyl-[F-actin] + NADPH + O2 = H2O + L-methionyl- (R)-S-oxide-[F-actin] + NADP(+) (RHEA:51308)
catalytic activity: H(+) + NADPH + O2 = H2O2 + NADP(+) (RHEA:11260)
cofactor: FAD
subunit: Associates with the SH3 domain of NEDD9. Interacts with VIM and PLXNA3. Interacts with RAB1B, RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); binding to RAB1B is of low affinity compared to other Rab proteins; at least in case of RAB8A and RAB10 can bind 2 molecules of the Rab proteins simultaneously (By similarity). Interacts with STK38 and STK38L. Interacts with GRAF1/ARHGAP26, GRAF2/ARHGAP10, RAB8A, RAB8B and RAB10; may bind simultaneously to GRAFs and Rabs and connects GRAFs to Rabs (By similarity). Does not interact with RAB1 and RAB11A (By similarity).
MICAL_DROME / Q86BA1 [F-actin]-monooxygenase Mical; Molecule interacting with CasL protein homolog; EC 1.14.13.225 from Drosophila melanogaster (Fruit fly) (see 5 papers)
Q86BA1 F-actin monooxygenase (EC 1.14.13.225) from Drosophila melanogaster (see 4 papers)
Aligns to 4563:4697 / 4723 (2.9%), covers 88.8% of PF12130, 88.7 bits
- function: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits at 'Met-44' and 'Met-47' through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament disassembly and prevent repolymerization. Plays a key role in semaphorin-plexin repulsive axon guidance and cell morphological changes, probably via its ability to modify and regulate actin.
catalytic activity: H(+) + L-methionyl-[F-actin] + NADPH + O2 = H2O + L-methionyl- (R)-S-oxide-[F-actin] + NADP(+) (RHEA:51308)
cofactor: FAD
subunit: Interacts with plexA.
disruption phenotype: Defects in motor neuron guidance, myofilament organization and bristle formation. Surviving adult flies show abnormally shaped bristle cell processes that are variously straight, thick, bent, twisted and/or had abnormal 'club-like' or blunt tips.
Or search for genetic data about PF12130 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory