Family Search for PF13427 (AadA_C)

Running HMMer for PF13427

PF13427 hits 47 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

DK877_09725 aminoglycoside nucleotidyltransferase ANT(9) from Streptococcus suis
Aligns to 149:251 / 258 (39.9%), covers 99.0% of PF13427, 120.8 bits

• Comparative virulence and antimicrobial resistance distribution of Streptococcus suis isolates obtained from the United States
  Nicholson, Frontiers in microbiology 2022
  • “...MGE Type AMR gene (locus_tag) All isolates containing representative MGE ID c1056_g113_s1056 17,428 IME ant(9)-Ia (DK877_09725), lnuB (DK877_09745), lsaE (DK877_09750) ISU2414 c1703_g122_s1703 32,277 IS Element, IME ermB (DK875_06640) ISU2514 c17669_g141_s5185 68,483 IME ermB (DK876_03150), tetO (DK876_03195) 29932, 30114, 30428, 37904, 40418, 40424, 40444, 40469, 40534, 40535,...”
• Comparative Virulence and Genomic Analysis of Streptococcus suis Isolates
  Nicholson, Frontiers in microbiology 2020
  • “...be located within a genomic island ( Supplementary Tables 2 , 3 ). The ANT(9) (DK877_09725), lnu(B) (DK877_09745), and lsaE (DK877_09750) genes from ISU2414 are collocated within the same chromosomal region, resembling an operon structure. This region encompasses six CDSs and is not located within any...”

S9AD_STAAU / P0A0D2 Spectinomycin 9-adenylyltransferase; AAD(9) from Staphylococcus aureus (see paper)
SA0049 O-nucleotidylltransferase(9) from Staphylococcus aureus subsp. aureus N315
SA0765 O-nucleotidylltransferase(9) from Staphylococcus aureus subsp. aureus N315
SA1481 O-nucleotidylltransferase(9) from Staphylococcus aureus subsp. aureus N315
SA1952 O-nucleotidylltransferase(9) from Staphylococcus aureus subsp. aureus N315
SA2385 O-nucleotidylltransferase(9) from Staphylococcus aureus subsp. aureus N315
SAV1656 O-nucleotidylltransferase from Staphylococcus aureus subsp. aureus Mu50
SERP2509 streptomycin 3''-adenylyltransferase from Staphylococcus epidermidis RP62A
EfmE1679_0213 aminoglycoside nucleotidyltransferase ANT(9)-Ia from Enterococcus faecium E1679
Aligns to 152:254 / 260 (39.6%), covers 98.1% of PF13427, 119.4 bits

• function: Mediates bacterial resistance to the antibiotic spectinomycin but not streptomycin.
  catalytic activity: ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate (RHEA:63228)
• Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica
  Stern, The Journal of biological chemistry 2018
  • “...marcescens (Q8VQN7) and P. aeruginosa (Q9RGC2); three ANT(9) enzymes from E. faecalis (Q07448), S. aureus (P0A0D2), and C. jejuni (Q4VR99); and one enzyme lacking ANT(3) and ANT(9) activity from C. jejuni (Q4VR96). Secondary structure of AadA is indicated above the alignment, colored as in Figs. 2...”
• The Genera Staphylococcus and Macrococcus
  , The ProkaryotesVolume 4: Bacteria: Firmicutes, Cyanobacteria 2006
  • “...SA1951 ermA Em resistance - rRNA adenine N -6-methyltransferase SA2348 ermA Em resistance - O-nucleotidyltransferase SA0049 ant Ags resistance b - O-nucleotidyltransferase SA0765 ant Ags resistance - O-nucleotidyltransferase SA1481 ant Ags resistance - O-nucleotidyltransferase SA1952 ant Ags resistance - O-nucleotidyltransferase SA2385 ant Ags resistance - Bifunctional...”
• Transcriptional profiling analysis of the global regulator NorG, a GntR-like protein of Staphylococcus aureus
  Truong-Bolduc, Journal of bacteriology 2011
  • “...SA2024 SA1450 SA0746 SA0246 SA0245 SA2056 SA2055 SA0766 SA0765 SA2287 SA0726 SA2384 SA2137 SA1639 SA1374 Change in expression level (n-fold)a Gene description...”
• Direct targets of CodY in Staphylococcus aureus
  Majerczyk, Journal of bacteriology 2010
  • “...saeRS Regulatory protein, putative Regulators SA2585 SA0767 SA0765 Virulence factors SA2026 SA2022 SA0136 SA0138 SA0139 SA0141 SA0142 SA0148 SA0149 SA0151...”
• Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
  Anderson, FEMS immunology and medical microbiology 2010
  • “...sa_c6155s5336_a_at * 4.4 2.5 2.5 lytS SA0245 sensor histidine kinase sa_c8186s7165_a_at 3.0 2.5 30 saeS SA0765 sensor histidine kinase sa_c831s632_a_at * 4.8 2.5 15 typA SA1118 GTP-binding protein sa_c8316s7293_a_at * 4.0 2.5 2.5 SA0809 GGDEF domain-containing protein Cell wall and membrane biogenesis sa_c10639s11094_s_at 4.4 2.5 30...”
• Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States
  Tenover, Journal of clinical microbiology 2006
  • “...saeR mgrA arlS arlR rot svrA srrA TRAP yycG SA0765 SA0766 SA0746 SA1450 SA1451 SA1812 SA0416 SA1535 SA1891 SA0020 Cell surface genes Amidase SA2666 Accessory...”
• The Genera Staphylococcus and Macrococcus
  , The ProkaryotesVolume 4: Bacteria: Firmicutes, Cyanobacteria 2006
  • “...N -6-methyltransferase SA2348 ermA Em resistance - O-nucleotidyltransferase SA0049 ant Ags resistance b - O-nucleotidyltransferase SA0765 ant Ags resistance - O-nucleotidyltransferase SA1481 ant Ags resistance - O-nucleotidyltransferase SA1952 ant Ags resistance - O-nucleotidyltransferase SA2385 ant Ags resistance - Bifunctional AAC/APH protein SAVP026 aacA Ags resistance -...”
• Comparative genomics of Staphylococcus aureus musculoskeletal isolates
  Cassat, Journal of bacteriology 2005
  • “...COL Affymetrix Affymetrix Affymetrix Affymetrix Affymetrix SA0766 SA0765 SA1450 SA1812 SA0672 SA0096 SA2287 SA2506 SA2507 SA2258 SA0416 SA1534 SA1891 SA0020...”
• Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
  Anderson, FEMS immunology and medical microbiology 2010
  • “...hypothetical protein sa_c2066s1777_a_at * 9.1 2.5 2.5 SA1468 hypothetical protein sa_c2109s1814_a_at * 23.2 2.5 ND SA1481 hypothetical protein sa_c2120s1823_a_at * 7.8 2.5 2.5 SA1483 hypothetical protein sa_c2481s2059_at 2.8 2.5 2.5 SA1556 hypothetical protein sa_c10583s11040_a_at * 2.2 2.5 ND SA1585 hypothetical protein sa_c2644s2217_a_at 5.0 2.5 30 SA1616...”
  • “...hypothetical protein sa_c2066s1777_a_at * 36.6 2.5 ND SA1468 hypothetical protein sa_c2109s1814_a_at * 29.3 2.5 ND SA1481 hypothetical protein sa_c2120s1823_a_at * 13.1 2.5 2.5 SA1483 hypothetical protein sa_c9740s8486_a_at 2.7 2.5 2.5 SA1485 hypothetical protein sa_c2182s1883_a_at 3.1 2.5 ND SA1501 hypothetical protein sa_c9728s8474_a_at 3.6 2.5 2.5 SA1512 hypothetical...”
• The Genera Staphylococcus and Macrococcus
  , The ProkaryotesVolume 4: Bacteria: Firmicutes, Cyanobacteria 2006
  • “...- O-nucleotidyltransferase SA0049 ant Ags resistance b - O-nucleotidyltransferase SA0765 ant Ags resistance - O-nucleotidyltransferase SA1481 ant Ags resistance - O-nucleotidyltransferase SA1952 ant Ags resistance - O-nucleotidyltransferase SA2385 ant Ags resistance - Bifunctional AAC/APH protein SAVP026 aacA Ags resistance - Tetracycline resistance protein SAV0387 tetM Tc...”
• Transcriptional profiling analysis of the global regulator NorG, a GntR-like protein of Staphylococcus aureus
  Truong-Bolduc, Journal of bacteriology 2011
  • “...SA0478 SA0517 SA1173 SA2553 SA1731 SA0172 SA0118 SA1952 SA1976 SA2386 SA0660 PTS system, IIABC components PTS system, IIBC components Phosphatidate...”
• Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
  Anderson, FEMS immunology and medical microbiology 2010
  • “...family protein sa_c9037s7935_a_at 8.0 2.5 2.5 SA1096 TrkA potassium uptake family sa_c3767s3239_at 6.1 2.5 stable SA1952 ferritins family protein sa_c5013s4315_a_at 2.2 2.5 2.5 SA2277 iron compound ABC transporter sa_c5160s4458_a_at * 7.1 2.5 2.5 SA2319 Na+/H+ antiporter family sa_c5522s4780_a_at * 5.9 2.5 15 SA2382 proton/sodium-glutamate symport sa_c5534s4791_a_at...”
• Global transcriptome analysis of Staphylococcus aureus response to hydrogen peroxide
  Chang, Journal of bacteriology 2006
  • “...(19). Next, the protein encoded by COL-SA0799 and -SA1952 was homologous to a ferritin family protein. This outcome is intriguing because ferritins are the...”
• The Genera Staphylococcus and Macrococcus
  , The ProkaryotesVolume 4: Bacteria: Firmicutes, Cyanobacteria 2006
  • “...b - O-nucleotidyltransferase SA0765 ant Ags resistance - O-nucleotidyltransferase SA1481 ant Ags resistance - O-nucleotidyltransferase SA1952 ant Ags resistance - O-nucleotidyltransferase SA2385 ant Ags resistance - Bifunctional AAC/APH protein SAVP026 aacA Ags resistance - Tetracycline resistance protein SAV0387 tetM Tc resistance - Bleomycin resistance protein SA0032...”
• Transcriptional profiling reveals that daptomycin induces the Staphylococcus aureus cell wall stress stimulon and genes responsive to membrane depolarization
  Muthaiyan, Antimicrobial agents and chemotherapy 2008
  • “...SA2017 SA0556 SA1271 groEL dnaK grpE groES NA hslU SA2385 NA SA1459 NA SA1034 NA Chaperonin, 60 kDa DnaK Heat shock protein GrpE Chaperonin, 10 kDa Chaperonin,...”
• Characterization of the Staphylococcus aureus heat shock, cold shock, stringent, and SOS responses and their effects on log-phase mRNA turnover
  Anderson, Journal of bacteriology 2006
  • “...13.0 4.6 6.0* cspB SA2731 SA1753 SA1759 SA2131 SA2385 Cold shock protein Universal stress protein family Universal stress protein family Dps family protein Heat...”
• The Genera Staphylococcus and Macrococcus
  , The ProkaryotesVolume 4: Bacteria: Firmicutes, Cyanobacteria 2006
  • “...resistance - O-nucleotidyltransferase SA1481 ant Ags resistance - O-nucleotidyltransferase SA1952 ant Ags resistance - O-nucleotidyltransferase SA2385 ant Ags resistance - Bifunctional AAC/APH protein SAVP026 aacA Ags resistance - Tetracycline resistance protein SAV0387 tetM Tc resistance - Bleomycin resistance protein SA0032 bleO Ble resistance - Symbol and...”
• Identification of gene clusters associated with host adaptation and antibiotic resistance in Chinese Staphylococcus aureus isolates by microarray-based comparative genomics
  Li, PloS one 2013
  • “...SAV0398 tetM 41 (85.4) 0 <0.0001 C11 SAV0052 ermA 42 (87.5) 4 (8.3) <0.0001 C12 SAV1656 ant (9) 35 (72.9) 6 (12.5) <0.0001 a Clusters that are not listed were not associated with antibiotic resistance. Comparative Genomics of Predominant MRSA Clones in China From 1994 to...”
• Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus
  Zhang, Antimicrobial agents and chemotherapy 2009
  • “...SERP2512 (RP62A) SERP2511 (RP62A) SERP2510 (RP62A) SERP2509 (RP62A) SERP2508 (RP62A) SERP2507 (RP62A) SERP2506 (RP62A) SERP2505 (RP62A) SERP2504 (RP62A);...”
• Complete genome sequence of Enterococcus faecium strain TX16 and comparative genomic analysis of Enterococcus faecium genomes
  Qin, BMC microbiology 2012
  • “...to the genes found on the E. faecalis plasmid pEF418 (Genbank:AF408195); aad9 (streptomycin 3-adenylyltransferase) using EfmE1679_0213 sequence and located on Tn 554 [ 103 ]; aadE (aminoglycoside 6-adenylyltransferase) using EfmU0317_2169 sequence an ortholog to the gene found on the E. faecalis plasmid pRE25 [ 104 ];...”

S3AD_ECOLX / P0AG05 Aminoglycoside (3'') (9) adenylyltransferase; Aminoglycoside 3''-adenylyltransferase; AAD(3'') (9) adenylyltransferase; Streptomycin 3''-adenylyltransferase; EC 2.7.7.47 from Escherichia coli (see 2 papers)
Q71UU1 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Enterococcus faecalis (see paper)
Aligns to 152:256 / 263 (39.9%), covers 98.1% of PF13427, 112.6 bits

• function: Mediates bacterial resistance to the antibiotics streptomycin and spectinomycin.
  catalytic activity: ATP + streptomycin = 3''-O-adenylylstreptomycin + diphosphate (RHEA:20245)
  catalytic activity: ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate (RHEA:63228)
  disruption phenotype: Loss of resistance to streptomycin and spectinomycin.
• Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica
  Stern, The Journal of biological chemistry 2018
  • “...AadA (Q8ZPX9; UniProt accession numbers in parentheses) with three characterized ANT(3)(9) enzymes from E. faecalis (Q71UU1), S. marcescens (Q8VQN7) and P. aeruginosa (Q9RGC2); three ANT(9) enzymes from E. faecalis (Q07448), S. aureus (P0A0D2), and C. jejuni (Q4VR99); and one enzyme lacking ANT(3) and ANT(9) activity from...”

C4NV14 Aminoglycoside (3'') (9) adenylyltransferase from Escherichia coli
Aligns to 169:273 / 280 (37.5%), covers 98.1% of PF13427, 112.6 bits

• Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase
  Chen, Acta crystallographica. Section D, Biological crystallography 2015
  • “...are given in parentheses): Salmonella bongori (F8VG00), Providencia stuartii (I0E075), Myxococcus xanthus (Q1DBM4), Escherichia coli (C4NV14), Serratia odorifera (D4E6F0), Variovorax paradoxus (C5CZ95), Pseudomonas mendocina (A4XUC2) and Enterococcus faecalis D6 (C7UVI9). Strictly conserved residues are highlighted in red and conservative substitutions are in red font. The secondary...”

Q79JG0 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Escherichia coli (see paper)
aadA1 / CAD29131.1 aminoglycoside adenyltransferase from Pseudomonas putida (see paper)
Aligns to 152:256 / 263 (39.9%), covers 98.1% of PF13427, 112.5 bits

Q5DUC3 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Escherichia coli (see paper)
Aligns to 148:252 / 259 (40.5%), covers 98.1% of PF13427, 112.4 bits

EF1076 streptomycin 3''-adenylyltransferase, putative from Enterococcus faecalis V583
Aligns to 156:257 / 261 (39.1%), covers 97.1% of PF13427, 111.2 bits

• Impact of manganese, copper and zinc ions on the transcriptome of the nosocomial pathogen Enterococcus faecalis V583
  Abrantes, PloS one 2011
  • “...seen responding to the other two metals, and four stress response related proteins (EF0298, EF1058, EF1076 and KatA). The cop operon ( ef0297-9 ), described as being responsible for copper uptake, availability and export in E. hirae [30] , was the highest induced operon, as previously...”
  • “...induced in the presence of zinc stress. Other genes encoding stress related proteins (EF0781, EF1058, EF1076, EF1084) were differentially transcribed under at least one of the metal stresses studied and ef1058 and ef1084 were also induced in blood [22] . Oxidative stress related genes, namely katA...”
• Large-scale screening of a targeted Enterococcus faecalis mutant library identifies envelope fitness factors
  Rigottier-Gois, PloS one 2011
  • “...(PTS system; ef0553 ), in cell envelope ( ef0252 ) and in cellular processes ( ef1076 ). In one of the adhesion-defective mutants, a hypothetical protein (EF0876) was affected that carries a domain found in the streptococcal regulator Mga, which is known to regulate production of...”
• Transcriptional response of Enterococcus faecalis V583 to erythromycin
  Aakra, Antimicrobial agents and chemotherapy 2005
  • “...EF2749 EF3056 EF3061 EF3062 EF3256 EF0079 EF0080 EF0097 EF0463 EF0639 EF0818 EF1058 EF1076 EF1182 EF1991 EF2677 0 0 0.84 0 0 0 0 0.95 0 0 0 0 0 0.86 0 0 0 0 0...”

7uy4A / P0AG05 Aminoglycoside-modifying enzyme ant-3,9 in complex with spectinomycin and amp-pnp
Aligns to 148:252 / 258 (40.7%), covers 98.1% of PF13427, 110.4 bits

• Ligands: phosphoaminophosphonic acid-adenylate ester; spectinomycin; magnesium ion (7uy4A)

C5CZ95 Aminoglycoside (3'') (9) adenylyltransferase from Variovorax paradoxus (strain S110)
Aligns to 152:255 / 269 (38.7%), covers 98.1% of PF13427, 108.7 bits

• Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase
  Chen, Acta crystallographica. Section D, Biological crystallography 2015
  • “...(F8VG00), Providencia stuartii (I0E075), Myxococcus xanthus (Q1DBM4), Escherichia coli (C4NV14), Serratia odorifera (D4E6F0), Variovorax paradoxus (C5CZ95), Pseudomonas mendocina (A4XUC2) and Enterococcus faecalis D6 (C7UVI9). Strictly conserved residues are highlighted in red and conservative substitutions are in red font. The secondary structure of S. enterica AadA is...”

P08881 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Klebsiella pneumoniae (see paper)
NP_608308 adenylyltranferase from Klebsiella pneumoniae
Aligns to 152:255 / 262 (39.7%), covers 98.1% of PF13427, 108.7 bits

• Klebsiella pneumoniae multiresistance plasmid pMET1: similarity with the Yersinia pestis plasmid pCRY and integrative conjugative elements
  Soler, PloS one 2008
  • “...NP_608309 32 33,10332,315 53.6 262 Adenylyltranferase [pJHCMW1, Tn1331 , Klebsiella pneumoniae ] * 6e-145 99/100 NP_608308 33 33,77833,173 54.1 201 Aminoglycoside 6-N-acetyltransferase type Ib (AAC(6)-Ib) * [pJHCMW1, Tn 1331 , Klebsiella pneumoniae ] 1e-112 100/100 NP_608307 34 35,31734,478 50.3 279 Beta-lactamase precursor * (Oxacillinase, OXA-9) [pJHCMW1,...”
  • “...NP_608309 35 36,15035,362 53.6 262 Adenylyltranferase [pJHCMW1, Tn1331 , Klebsiella pneumoniae ] * 6e-145 99/100 NP_608308 36 36,82536,220 54.1 201 Aminoglycoside 6-N-acetyltransferase type Ib (AAC(6)-Ib) * [pJHCMW1, Tn 1331 , Klebsiella pneumoniae ] 1e-112 100/100 NP_608307 37 37,56537,008 54.3 185 Resolvase [pJHCMW1, Tn1331 , Klebsiella pneumoniae...”

S3AD_SHIFL / P0AG06 Aminoglycoside (3'') (9) adenylyltransferase; Streptomycin 3''-adenylyltransferase; EC 2.7.7.47 from Shigella flexneri (see paper)
A1J85_26735, A1Q75_26310, AW170_26955, AYK88_26945, B7L32_25510 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 from Klebsiella aerogenes
Aligns to 152:255 / 262 (39.7%), covers 98.1% of PF13427, 108.7 bits

• function: Mediates bacterial resistance to the antibiotics streptomycin and spectinomycin.
  catalytic activity: ATP + streptomycin = 3''-O-adenylylstreptomycin + diphosphate (RHEA:20245)
  catalytic activity: ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate (RHEA:63228)
• In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract
  da, Frontiers in microbiology 2018
  • “...C3F38_25945), and carbapenems (KPC-2, C3F38_25920). On the other hand, pRPA1 plasmid encodes the aadA1 gene (B7L32_25510) for aminoglycoside resistance also found in pKPC-56ce, but not in pRPH1. The coprophagic behavior of triatomines might explain the variability of S. marcescens populations in the digestive tract of specimens...”
• Genome Sequencing of Four Multidrug-Resistant Enterobacter aerogenes Isolates from Hospitalized Patients in Brazil
  Grazziotin, Frontiers in microbiology 2016
  • “...(AacC3) P0A255.1 AW170_26910 AYK88_26960 ANT(3)-Ia family aminoglycoside nucleotidyltransferase AadA WP_014325834.1 AW170_26955 AYK88_26945 A1Q75_26045 A1Q75_26310 A1J85_26670 A1J85_26735 Chloramphenicol acetyltransferase III (Cat3) P00484.1 AW170_27070 AYK88_27075 Class A beta-lactamase - Beta-lactamase CTX-M-6 O65976.1 AW170_27050 AYK88_27040 A1Q75_26225 Class A beta-lactamase - TEM family WP_010331504.1 WP_000027063.1 AW170_26915 AW170_27230 AYK88_27065 AYK88_27140 A1Q75_26300...”
  • “...N(3)-acetyltransferase III (AacC3) P0A255.1 AW170_26910 AYK88_26960 ANT(3)-Ia family aminoglycoside nucleotidyltransferase AadA WP_014325834.1 AW170_26955 AYK88_26945 A1Q75_26045 A1Q75_26310 A1J85_26670 A1J85_26735 Chloramphenicol acetyltransferase III (Cat3) P00484.1 AW170_27070 AYK88_27075 Class A beta-lactamase - Beta-lactamase CTX-M-6 O65976.1 AW170_27050 AYK88_27040 A1Q75_26225 Class A beta-lactamase - TEM family WP_010331504.1 WP_000027063.1 AW170_26915 AW170_27230 AYK88_27065...”
  • “...A1Q75_26315 A1J85_26740 Aminoglycoside N(3)-acetyltransferase III (AacC3) P0A255.1 AW170_26910 AYK88_26960 ANT(3)-Ia family aminoglycoside nucleotidyltransferase AadA WP_014325834.1 AW170_26955 AYK88_26945 A1Q75_26045 A1Q75_26310 A1J85_26670 A1J85_26735 Chloramphenicol acetyltransferase III (Cat3) P00484.1 AW170_27070 AYK88_27075 Class A beta-lactamase - Beta-lactamase CTX-M-6 O65976.1 AW170_27050 AYK88_27040 A1Q75_26225 Class A beta-lactamase - TEM family WP_010331504.1 WP_000027063.1...”
  • “...A1J85_26740 Aminoglycoside N(3)-acetyltransferase III (AacC3) P0A255.1 AW170_26910 AYK88_26960 ANT(3)-Ia family aminoglycoside nucleotidyltransferase AadA WP_014325834.1 AW170_26955 AYK88_26945 A1Q75_26045 A1Q75_26310 A1J85_26670 A1J85_26735 Chloramphenicol acetyltransferase III (Cat3) P00484.1 AW170_27070 AYK88_27075 Class A beta-lactamase - Beta-lactamase CTX-M-6 O65976.1 AW170_27050 AYK88_27040 A1Q75_26225 Class A beta-lactamase - TEM family WP_010331504.1 WP_000027063.1 AW170_26915...”

Q51348 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Escherichia coli (see paper)
Aligns to 148:252 / 259 (40.5%), covers 97.1% of PF13427, 106.8 bits

S3AD_RHIRD / P14511 Aminoglycoside (3'') (9) adenylyltransferase; Streptomycin 3''-adenylyltransferase; SP-R; EC 2.7.7.47 from Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter) (see 2 papers)
Aligns to 158:262 / 269 (39.0%), covers 97.1% of PF13427, 106.7 bits

• function: Mediates bacterial resistance to the antibiotic spectinomycin and probably also to streptomycin.
  catalytic activity: ATP + streptomycin = 3''-O-adenylylstreptomycin + diphosphate (RHEA:20245)
  catalytic activity: ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate (RHEA:63228)
  disruption phenotype: Loss of resistance to spectinomycin.

A1J85_26670, A1Q75_26045 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA2 from Klebsiella aerogenes
YpIP275_pIP1202_0190 aminoglycoside resistance protein from Yersinia pestis biovar Orientalis str. IP275
Aligns to 152:256 / 263 (39.9%), covers 97.1% of PF13427, 106.7 bits

• Genome Sequencing of Four Multidrug-Resistant Enterobacter aerogenes Isolates from Hospitalized Patients in Brazil
  Grazziotin, Frontiers in microbiology 2016
  • “...III (AacC3) P0A255.1 AW170_26910 AYK88_26960 ANT(3)-Ia family aminoglycoside nucleotidyltransferase AadA WP_014325834.1 AW170_26955 AYK88_26945 A1Q75_26045 A1Q75_26310 A1J85_26670 A1J85_26735 Chloramphenicol acetyltransferase III (Cat3) P00484.1 AW170_27070 AYK88_27075 Class A beta-lactamase - Beta-lactamase CTX-M-6 O65976.1 AW170_27050 AYK88_27040 A1Q75_26225 Class A beta-lactamase - TEM family WP_010331504.1 WP_000027063.1 AW170_26915 AW170_27230 AYK88_27065 AYK88_27140...”
  • “...Aminoglycoside N(3)-acetyltransferase III (AacC3) P0A255.1 AW170_26910 AYK88_26960 ANT(3)-Ia family aminoglycoside nucleotidyltransferase AadA WP_014325834.1 AW170_26955 AYK88_26945 A1Q75_26045 A1Q75_26310 A1J85_26670 A1J85_26735 Chloramphenicol acetyltransferase III (Cat3) P00484.1 AW170_27070 AYK88_27075 Class A beta-lactamase - Beta-lactamase CTX-M-6 O65976.1 AW170_27050 AYK88_27040 A1Q75_26225 Class A beta-lactamase - TEM family WP_010331504.1 WP_000027063.1 AW170_26915 AW170_27230...”
• Yersinia pestis antibiotic resistance: a systematic review
  Lei, Osong public health and research perspectives 2022
  • “...class D (plasmid) 152529 153368 sul1 YpIP275_pIP1202_0188 ABO42234.1 279 Dihydropteroate synthase (plasmid) 153873 154883 aadA YpIP275_pIP1202_0190 ABO42050.1 336 Aminoglycoside adenylyltransferase (plasmid) S19960127 Chromosome NZ_CP045640.1 4.55 47.5 3849 4461357 4461731 rpsL S96127_RS21075 WP_200911418.1 124 30S ribosomal protein S12 16/95 Plasmid pIP1203 HG326189 0.047 64.5 50 612 1415...”
• Proteomic Signatures of Antimicrobial Resistance in Yersinia pestis and Francisella tularensis
  Deatherage, Frontiers in medicine 2022
  • “...No YpIP275_pIP1202_0063 Chloramphenicol acetyltransferase Chloramphenicol No No YpIP275_pIP1202_0055 Streptomycin resistance protein A Kanamycin No Yes YpIP275_pIP1202_0190 Aminoglycoside 3-adenylyltransferase Streptomycin and spectinomycin Yes Yes YpIP275_pIP1202_0188 Dihydropteroate synthase (Sul1) Sulfamethoxazole Yes No YpIP275_pIP1202_0073 Dihydropteroate synthase (Sul2) Sulfamethoxazole Yes Yes AMR Status Influences Similar Cell Functions and Pathways in...”

Q9F7C0 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Escherichia coli (see paper)
Aligns to 152:256 / 263 (39.9%), covers 97.1% of PF13427, 106.7 bits

Q5UNL3 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Escherichia coli (see paper)
Aligns to 152:256 / 263 (39.9%), covers 98.1% of PF13427, 105.7 bits

Q9R3A9 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Escherichia coli (see paper)
aafa5 / CAI46943.1 aminoglycoside-3''-adenylyltransferase from Pseudomonas aeruginosa (see paper)
Q7BPB1 Aminoglycoside (3'') (9) adenylyltransferase from Klebsiella pneumoniae
Aligns to 152:256 / 262 (40.1%), covers 98.1% of PF13427, 104.9 bits

• Evaluation of Live Bacterial Prophylactics to Decrease IncF Plasmid Transfer and Association With Intestinal Small RNAs.
  Redweik, Frontiers in microbiology 2020
  • “...pilus assembly protein; P41067 0.00027 12237-5p 45.4 Novel, function NYD; A aadA4 Aminoglycoside 3-adenylyltransferase activity; Q7BPB1 0.00053 12237-5p 45.2 Novel, function NYD; A traW Involved in F-pilus assembly. Required for F plasmid conjugative transfer; P18472 0.00033 12267-3p 44.1 Novel, function NYD; A traP Conjugal transfer protein...”

Q59694 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Pseudomonas aeruginosa (see paper)
Aligns to 148:252 / 259 (40.5%), covers 97.1% of PF13427, 103.9 bits

A4XUC2 Aminoglycoside (3'') (9) adenylyltransferase from Pseudomonas mendocina (strain ymp)
Aligns to 180:284 / 295 (35.6%), covers 98.1% of PF13427, 102.0 bits

• Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase
  Chen, Acta crystallographica. Section D, Biological crystallography 2015
  • “...(I0E075), Myxococcus xanthus (Q1DBM4), Escherichia coli (C4NV14), Serratia odorifera (D4E6F0), Variovorax paradoxus (C5CZ95), Pseudomonas mendocina (A4XUC2) and Enterococcus faecalis D6 (C7UVI9). Strictly conserved residues are highlighted in red and conservative substitutions are in red font. The secondary structure of S. enterica AadA is shown above the...”

Q9L799 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Escherichia coli (see paper)
Aligns to 152:256 / 265 (39.6%), covers 97.1% of PF13427, 99.9 bits

SD3246_2037 cryptic aminoglycoside nucleotidyltransferase ANT(3'')/ANT(9) from Salmonella enterica subsp. enterica serovar Dublin str. SD3246
Aligns to 152:256 / 262 (40.1%), covers 98.1% of PF13427, 99.0 bits

• On the evolutionary history, population genetics and diversity among isolates of Salmonella Enteritidis PFGE pattern JEGX01.0004
  Allard, PloS one 2013
  • “...enterica subsp. enterica serovar Enteritidis str. P125109] Length=296 32 571_input.aln 571_protein.fas 251 CT 84 PL SD3246_2037 SD3246_2037 ref|ZP_09764523.1| aminoglycoside resistance protein [Salmonella enterica subsp. enterica serovar Dublin str. SD3246] Length=289 33 589_input.aln 589_protein.fas 848 GA 283 GE SEN0539 SEN0539 ref|YP_002242687.1| AraC family transcriptional regulator [Salmonella enterica...”

S3AD_SALTY / Q8ZPX9 Aminoglycoside (3'') (9) adenylyltransferase; AadA; Aminoglycoside nucleotidyltransferase (3'') (9) adenyltransferase; ANT(3'') (9) adenyltransferase; EC 2.7.7.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 3 papers)
Q8ZPX9 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Salmonella enterica subsp. enterica serovar Typhimurium (see paper)
STM1264 Aminoglycoside adenyltransferase from Salmonella typhimurium LT2
NP_460230 aminoglycoside adenyltransferase from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Aligns to 152:256 / 262 (40.1%), covers 98.1% of PF13427, 98.9 bits

• function: Mediates bacterial resistance to the antibiotics streptomycin and spectinomycin, does not confer resistance to kanamycin (PubMed:26527143). Binds ATP first, then antibiotic (PubMed:26527143, PubMed:29871922).
  catalytic activity: ATP + streptomycin = 3''-O-adenylylstreptomycin + diphosphate (RHEA:20245)
  catalytic activity: ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate (RHEA:63228)
  subunit: Monomer.
  disruption phenotype: Loss of streptomycin and spectinomycin resistance.
• Biofilm Bacteria Use Stress Responses to Detect and Respond to Competitors
  Lories, Current biology : CB 2020
  • “...guaC promoter GMP reductase stringent response STM0141 aadA promoter aminoglycoside adenyltransferase; involved in aminoglycoside resistance STM1264 invF promoter AraC-family regulatory protein STM2899 hilC promoter AraC-family transcriptional regulator STM2867 csgB promoter nucleation component of curli monomers STM1143 tolC promoter outer membrane porin; outer membrane component of several...”
• Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica.
  Stern, The Journal of biological chemistry 2018
  • GeneRIF: This study reports the crystal structures of WT aadA complexed with ATP, magnesium, and streptomycin and of an active-site mutant, E87Q, complexed with ATP and streptomycin or the closely related dihydrostreptomycin.
• Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase.
  Chen, Acta crystallographica. Section D, Biological crystallography 2015
  • GeneRIF: AadA consists of a nucleotidyltransferase domain and an alpha-helical bundle domain.
• A Novel Workflow for In Silico Prediction of Bioactive Peptides: An Exploration of Solanum lycopersicum By-Products
  Morena, Biomolecules 2024
  • “...S11, L14, G15, L18, N19 Target: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; UniProt: Q8ZPX9, PDB: 6FZB. * Residues of the active domain S36, S46, D47, D49, E87, D130, W173D178, H185, K205, and Y231. DAGASKTYPQQAGTIRKGGHIVIKNRP Q9AXQ6 Eukaryotic translation initiation factor 5A-1 27 32.07 1.49 D311,...”
• Identification of a novel aminoglycoside O-nucleotidyltransferase AadA33 in Providencia vermicola.
  Feng, Frontiers in microbiology 2022
  • “...51.09%, 49.37%, 47.22% and 45.77% amino acid identities with the function characterized AadA31 (AUX81654.1), AadA (Q8ZPX9), AadA5 (AAF17880.1), AadA10 (AAL36430.1) and AadA13 (ABW91178.1), respectively. To analyze the resistance function-related structural mechanism of AadA33, multiple sequence alignment of AadA33 and the function-characterized AadA proteins including the structure-characterized...”
  • “...corresponding sequence length. The red frames indicate functional residues. Accession numbers of AadA proteins: AadA (Q8ZPX9), AadA31 (AUX81654.1), AadA10 (AAL36430.1), AadA13 (ABW91178.1) and AadA5 (AAF17880.1). To figure out the genetic context of aadA33 , the sequences of about 20kb in length with an aadA33 -like gene...”
• AadA36, a novel chromosomal aminoglycoside nucleotidyltransferase from a clinical isolate of Providencia stuartii.
  Gao, Frontiers in microbiology 2022
  • “...was assigned as a novel lineage of the ANT(3)-Ia family. The structural mechanism of AadA (Q8ZPX9) has been verified that the determinants for adenylation activity on streptomycin were amino acid residues W173 and D178, and on spectinomycin were E87, W112, D182, and 185H/N. Besides, the last...”
• Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica
  Stern, The Journal of biological chemistry 2018
  • “...ANT(9) activity were 38% or higher. Figure 5. Multiple sequence alignment of S. enterica AadA (Q8ZPX9; UniProt accession numbers in parentheses) with three characterized ANT(3)(9) enzymes from E. faecalis (Q71UU1), S. marcescens (Q8VQN7) and P. aeruginosa (Q9RGC2); three ANT(9) enzymes from E. faecalis (Q07448), S. aureus...”

5lpaA / Q8ZPX9 Aada e87q in complex with atp, calcium and dihydrostreptomycin (see paper)
Aligns to 151:255 / 267 (39.3%), covers 98.1% of PF13427, 98.9 bits

• Ligands: adenosine-5'-triphosphate; dihydrostreptomycin; calcium ion (5lpaA)

SBOV12521 cryptic aminoglycoside nucleotidyltransferase ANT(3'')/ANT(9) from Salmonella enterica subsp. enterica serovar Bovismorbificans str.
Aligns to 152:256 / 262 (40.1%), covers 98.1% of PF13427, 98.6 bits

• Genomic characterisation of invasive non-typhoidal Salmonella enterica Subspecies enterica Serovar Bovismorbificans isolates from Malawi
  Bronowski, PLoS neglected tropical diseases 2013
  • “...Mechanism of resistance Resistance to * SBOV CDS DT104 CDS aadA streptomycin/spectinomycin adenyltransferase SPT, STR SBOV12521 SDT1236 aphA aminoglycoside phosphotransferase KAN SBOV43311 SDT4236 dhfr1 dihydrofolate reductase TMP SBOV00351 SDT0092 putative -lactamase -lactamases PENs SBOV25431 SDT2527 -lactamase domain SBOV29851 SDT2944 putative -lactamase SBOV38301 SDT3717 This table summarizes...”

Q02865 Aminoglycoside (3'') (9) adenylyltransferase from Salmonella enterica
Aligns to 152:256 / 262 (40.1%), covers 98.1% of PF13427, 98.5 bits

• Food for Thought: Proteomics for Meat Safety
  Tarbeeva, Life (Basel, Switzerland) 2023
  • “...Q7BD42, A0A3K0TB41 * Chloramphenicol [ 52 ] strA, strB and aadA A0A5C2D198 *, C4NVB8 *, Q02865 Streptomycin [ 52 ] sul1 and sul2 A0A0F6NWV0 *, A0A1S6KR61 * Sulfizoxazole [ 52 ] AcrAB/TolC, including acetylated lysine (PTM) A0A759DN94 * Fluoroquinolone [ 54 ] Quaternary ammonium compounds, chloramphenicol,...”

Q9RGC2 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Pseudomonas aeruginosa (see paper)
PAMH19_6401 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA6 from Pseudomonas aeruginosa
Aligns to 152:256 / 281 (37.4%), covers 98.1% of PF13427, 98.1 bits

• Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica
  Stern, The Journal of biological chemistry 2018
  • “...with three characterized ANT(3)(9) enzymes from E. faecalis (Q71UU1), S. marcescens (Q8VQN7) and P. aeruginosa (Q9RGC2); three ANT(9) enzymes from E. faecalis (Q07448), S. aureus (P0A0D2), and C. jejuni (Q4VR99); and one enzyme lacking ANT(3) and ANT(9) activity from C. jejuni (Q4VR96). Secondary structure of AadA...”
• Biophysical and enzymatic properties of aminoglycoside adenylyltransferase AadA6 from Pseudomonas aeruginosa
  Papadovasilaki, Biochemistry and biophysics reports 2015
  • “...to the subclass of 3-O-nucleotidyltransferases ( ANT 3), it consists of 281 amino-acid residues (UniProt: Q9RGC2) and confers resistance to streptomycin and spectinomycin [16] . The sequence information collected from the Conserved Domain Database (CDD) [18] for this protein points to the conserved nucleotidyltransferase (NTase) domain...”
• Genome Sequence of the Urethral Catheter Isolate Pseudomonas aeruginosa MH19
  Vorhölter, Genome announcements 2015
  • “...cadR (PAMH19_6165); and a lead-cadmium transporter, CadA (PAMH19_6073). In addition, the aminoglycoside resistance gene aadA (PAMH19_6401) contributed to the resistance pattern of P. aeruginosa MH19. An enhanced virulence of MH19 was mirrored by a pyocin gene (PAMH19_1526). Genes homologous to the zona occludens toxin gene from...”

Q1DBM4 Aminoglycoside-3'-adenylyltransferase from Myxococcus xanthus (strain DK1622)
Aligns to 152:256 / 272 (38.6%), covers 96.1% of PF13427, 97.2 bits

• Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase
  Chen, Acta crystallographica. Section D, Biological crystallography 2015
  • “...(UniProt accession numbers are given in parentheses): Salmonella bongori (F8VG00), Providencia stuartii (I0E075), Myxococcus xanthus (Q1DBM4), Escherichia coli (C4NV14), Serratia odorifera (D4E6F0), Variovorax paradoxus (C5CZ95), Pseudomonas mendocina (A4XUC2) and Enterococcus faecalis D6 (C7UVI9). Strictly conserved residues are highlighted in red and conservative substitutions are in red...”

Q8VQN7 aminoglycoside 6'-N-acetyltransferase (EC 2.3.1.82) from Serratia marcescens (see paper)
Aligns to 152:256 / 463 (22.7%), covers 97.1% of PF13427, 96.2 bits

• Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica
  Stern, The Journal of biological chemistry 2018
  • “...accession numbers in parentheses) with three characterized ANT(3)(9) enzymes from E. faecalis (Q71UU1), S. marcescens (Q8VQN7) and P. aeruginosa (Q9RGC2); three ANT(9) enzymes from E. faecalis (Q07448), S. aureus (P0A0D2), and C. jejuni (Q4VR99); and one enzyme lacking ANT(3) and ANT(9) activity from C. jejuni (Q4VR96)....”
• Biophysical and enzymatic properties of aminoglycoside adenylyltransferase AadA6 from Pseudomonas aeruginosa
  Papadovasilaki, Biochemistry and biophysics reports 2015
  • “...domain [ANT(3)-Ii] of the bifunctional, nucleotidyl- and acetyl-transferase aminoglycoside modifying enzyme from Serratia marcescens (UniProt: Q8VQN7), which has been extensively characterized with regard to its enzymatic properties [24] . The high homology between the enzymes suggests that AadA6 acts in a similar fashion. We decided to...”

BCAM1013a aminoglycoside-3''-adenylyltransferase from Burkholderia cenocepacia J2315
Aligns to 152:256 / 263 (39.9%), covers 96.1% of PF13427, 94.5 bits

• Genomic expression analysis reveals strategies of Burkholderia cenocepacia to adapt to cystic fibrosis patients' airways and antimicrobial therapy
  Mira, PloS one 2011
  • “...from genes encoding enzymes required for inactivation of aminoglycosides (the aminoglycoside-3-phosphotransferase BCAM0928 and the aminoglycoside-3adenyltransferase BCAM1013A ). The transcription of a penicillin-binding protein (PBP), BCAM1738 , and of a PBP-precursor, BCAL2205 , was down-regulated (by 1.8 and 1.6-fold, respectively) in IST4113 ( Table 2 ). Since...”

S9AD_CAMJU / Q4VR99 Spectinomycin 9-adenylyltransferase; ORF8 from Campylobacter jejuni (see paper)
Aligns to 148:250 / 258 (39.9%), covers 100.0% of PF13427, 94.1 bits

• function: Mediates bacterial resistance to spectinomycin, is probably a spectinomycin 9-adenylyltransferase.
  catalytic activity: ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate (RHEA:63228)
  disruption phenotype: Loss of spectinomycin resistance.
• Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica
  Stern, The Journal of biological chemistry 2018
  • “...aeruginosa (Q9RGC2); three ANT(9) enzymes from E. faecalis (Q07448), S. aureus (P0A0D2), and C. jejuni (Q4VR99); and one enzyme lacking ANT(3) and ANT(9) activity from C. jejuni (Q4VR96). Secondary structure of AadA is indicated above the alignment, colored as in Figs. 2 and 3 . Colored...”

D4E6F0 Aminoglycoside (3'') (9) adenylyltransferase from Serratia odorifera DSM 4582
Aligns to 188:292 / 310 (33.9%), covers 96.1% of PF13427, 90.3 bits

• Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase
  Chen, Acta crystallographica. Section D, Biological crystallography 2015
  • “...parentheses): Salmonella bongori (F8VG00), Providencia stuartii (I0E075), Myxococcus xanthus (Q1DBM4), Escherichia coli (C4NV14), Serratia odorifera (D4E6F0), Variovorax paradoxus (C5CZ95), Pseudomonas mendocina (A4XUC2) and Enterococcus faecalis D6 (C7UVI9). Strictly conserved residues are highlighted in red and conservative substitutions are in red font. The secondary structure of S....”

WP_004260670 aminoglycoside nucleotidyltransferase ANT9 from Providencia rettgeri DSM 1131
Aligns to 149:249 / 254 (39.8%), covers 94.2% of PF13427, 88.5 bits

• Genomic Plasticity of Multidrug-Resistant NDM-1 Positive Clinical Isolate of Providencia rettgeri
  Olaitan, Genome biology and evolution 2016
  • “...100 P10051 Chromosome (ICE) aphA-6 100 YP_005351836 Plasmid aadA1 100 YP_007878589 Chromosome (ICE) aadA 62 WP_004260670 Chromosome aac 65 Q52424 Chromosome Beta-lactam resistance: amoxicillin, amoxicillinclavulanic acid, ticarcillinclavulanic acid, cefalotine, cefoxitin, cefotaxime, ceftriaxone, ceftazidime, cefepime, piperacillin, piperacillintazobactam, imipenem blaOXA-10 100 WP_020442392 Chromosome (ICE) blaNDM-1 100 YP_005351834 Plasmid...”

Y9645_CAMJU / Q4VR96 Probable nucleotidyltransferase; ORF11; EC 2.7.7.- from Campylobacter jejuni (see paper)
Aligns to 152:251 / 264 (37.9%), covers 97.1% of PF13427, 80.5 bits

• function: Probably a nucleotidyltransferase, it does not confer spectinomycin or streptomyin resistance in situ or in E.coli.
  disruption phenotype: No change in spectinomycin or streptomycin resistance.
• Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica
  Stern, The Journal of biological chemistry 2018
  • “...and C. jejuni (Q4VR99); and one enzyme lacking ANT(3) and ANT(9) activity from C. jejuni (Q4VR96). Secondary structure of AadA is indicated above the alignment, colored as in Figs. 2 and 3 . Colored dots indicate contacts with ligands; corresponding sequence conservations are marked in the...”

WP_223854774 ANT(3'')-I family aminoglycoside nucleotidyltransferase AadA36 from Providencia stuartii
Aligns to 154:257 / 264 (39.4%), covers 92.2% of PF13427, 74.4 bits

• AadA36, a novel chromosomal aminoglycoside nucleotidyltransferase from a clinical isolate of Providencia stuartii
  Gao, Frontiers in microbiology 2022
  • “...QET96424.1, WP_004919927.1, WP_014658385.1, WP_040133143.1, WP_071821194.1, WP_071881681.1, WP_076913984.1, WP_102780545.1, WP_121875301.1, WP_141173405.1, WP_154610284.1, WP_154611447.1, WP_154625583.1, WP_174822199.1, WP_213537665.1, WP_223854774. Exclamations indicate fully conserved residues; asterisks indicate strongly similar residues. The numbers on the right represent the corresponding sequence length. Click here for additional data file. SUPPLEMENTARY FIGURE S2 A...”

AAW29_01543, AAW30_00055, AAX28_00760, AAX30_01180 aminoglycoside adenylyltransferase family protein from Arcobacter porcinus
Aligns to 154:257 / 262 (39.7%), covers 96.1% of PF13427, 72.6 bits

• Characterization of the emerging zoonotic pathogen Arcobacter thereius by whole genome sequencing and comparative genomics
  Rovetto, PloS one 2017
  • “...adenylyltransferase ( aadA25; AAD) was found in the genomes of A . thereius strains 213 (AAW29_01543), 216 (AAW30_00055), DU19 (AAX30_01180), and 117434 (AAX28_00760), which is involved in resistance towards streptomycin/spectinomycin in Pasteurella multocida [ 86 ]. Furthermore, a gene encoding an energy-depended membrane-associated protein TetA (AAX28_02020)...”
  • “...aadA25; AAD) was found in the genomes of A . thereius strains 213 (AAW29_01543), 216 (AAW30_00055), DU19 (AAX30_01180), and 117434 (AAX28_00760), which is involved in resistance towards streptomycin/spectinomycin in Pasteurella multocida [ 86 ]. Furthermore, a gene encoding an energy-depended membrane-associated protein TetA (AAX28_02020) was found...”
  • “...the genomes of A . thereius strains 213 (AAW29_01543), 216 (AAW30_00055), DU19 (AAX30_01180), and 117434 (AAX28_00760), which is involved in resistance towards streptomycin/spectinomycin in Pasteurella multocida [ 86 ]. Furthermore, a gene encoding an energy-depended membrane-associated protein TetA (AAX28_02020) was found in the genome of A...”
  • “...was found in the genomes of A . thereius strains 213 (AAW29_01543), 216 (AAW30_00055), DU19 (AAX30_01180), and 117434 (AAX28_00760), which is involved in resistance towards streptomycin/spectinomycin in Pasteurella multocida [ 86 ]. Furthermore, a gene encoding an energy-depended membrane-associated protein TetA (AAX28_02020) was found in the...”

N8W714 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Acinetobacter sp. NIPH 758 (IIb) (see paper)
Aligns to 144:249 / 263 (40.3%), covers 96.1% of PF13427, 71.7 bits

S9AD_ENTFL / Q07448 Spectinomycin 9-adenylyltransferase; AAD(9) from Enterococcus faecalis (Streptococcus faecalis) (see paper)
Aligns to 152:251 / 255 (39.2%), covers 97.1% of PF13427, 71.2 bits

• function: Mediates bacterial resistance to the antibiotic spectinomycin but not streptomycin.
  catalytic activity: ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate (RHEA:63228)
• Structural Recognition of Spectinomycin by Resistance Enzyme ANT(9) from Enterococcus faecalis
  Kanchugal, Antimicrobial agents and chemotherapy 2020 (secret)
• Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica
  Stern, The Journal of biological chemistry 2018
  • “...faecalis (Q71UU1), S. marcescens (Q8VQN7) and P. aeruginosa (Q9RGC2); three ANT(9) enzymes from E. faecalis (Q07448), S. aureus (P0A0D2), and C. jejuni (Q4VR99); and one enzyme lacking ANT(3) and ANT(9) activity from C. jejuni (Q4VR96). Secondary structure of AadA is indicated above the alignment, colored as...”

6xz0A / Q07448 Crystal structure of spectinomycin adenyltransferase aad(9) from enterococcus faecialis with spectinomycin (see paper)
Aligns to 148:247 / 250 (40.0%), covers 97.1% of PF13427, 71.2 bits

• Ligand: spectinomycin (6xz0A)

A1S_0131 putative adenylyltransferase from Acinetobacter baumannii ATCC 17978
Aligns to 102:205 / 218 (47.7%), covers 95.1% of PF13427, 70.3 bits

• Acinetobacter baumannii OxyR Regulates the Transcriptional Response to Hydrogen Peroxide
  Juttukonda, Infection and immunity 2019
  • “...A1S_0463 A1S_2459 A1S_3222 A1S_2293 A1S_1446 A1S_0131 Putative acyl-CoA dehydrogenase Putative acyl-CoA dehydrogenase FMN reductase Putative monooxygenase...”

ABTJ_RS18145 ANT(3'')-II family aminoglycoside nucleotidyltransferase from Acinetobacter baumannii MDR-TJ
Aligns to 146:249 / 262 (39.7%), covers 95.1% of PF13427, 69.9 bits

• Comparative genomic analysis and multi-drug resistance differences of Acinetobacter baumannii in Chongqing, China
  Pu, Infection and drug resistance 2019
  • “...kinase AdeS 1 NS efflux pump family ABTJ_RS05970 LysR family transcriptional regulator 1 NS Carbapenem ABTJ_RS18145 ANT (3)-II family aminoglycoside nucleotidyltransferase 49 NS Aminoglycoside ABTJ_RS05640 class C extended-spectrum beta-lactamase ADC-82 1 NS Carbapenem ABTJ_RS09235 two-component sensor histidine kinase AdeS 1 NS efflux pump family ABZJ_RS13265 QacE...”

D0CEM6 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Acinetobacter baumannii (see paper)
FQU82_00218, GO593_07260 aminoglycoside nucleotidyltransferase ANT(3'')-IIa from Acinetobacter baumannii
Aligns to 146:249 / 262 (39.7%), covers 95.1% of PF13427, 69.9 bits

• Complete genome sequence and genome-scale metabolic modelling of Acinetobacter baumannii type strain ATCC 19606
  Zhu, International journal of medical microbiology : IJMM 2020
  • “...to drive the expression of these genes ( Perichon et al., 2014 ). Genes ant(3)-IIa (GO593_07260) and sul2 (GO593_06680) were associated with resistance to streptomycin and sulphonamides, respectively. Compared to IC I-III strains, ATCC 19606 contains significantly less genes associated with the resistance to aminoglycosides and...”
• Analysis of Complete Genome Sequence of Acinetobacter baumannii Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
  Hamidian, Microorganisms 2020
  • “...a copy of the intrinsic ant(3)-IIa aminoglycoside resistance gene (located at bases 225025225813; locus id FQU82_00218), accounting for the streptomycin and spectinomycin resistance phenotypes observed ( Table 1 ). This intrinsic ant(3)-IIa aminoglycoside resistance gene is also present in the other two complete genomes. 3.3. Tn6551,...”

CHQ89_16705 ANT(3'')-II family aminoglycoside nucleotidyltransferase from Acinetobacter baumannii
Aligns to 146:249 / 262 (39.7%), covers 95.1% of PF13427, 69.9 bits

• Genotypic and Phenotypic Characterization of Novel Sequence Types of Carbapenem-Resistant Acinetobacter baumannii, With Heterogeneous Resistance Determinants and Targeted Variations in Efflux Operons
  Bharathi, Frontiers in microbiology 2021
  • “...ANT(3)-IIc gene that confers resistance to streptomycin, spectinomycin, was detected in A. baumannii strains DR1 CHQ89_16705, DR2 CHQ90_08400, and AB067 A8A08_02265 respectively, and these genes were found in the genomic island region. The aminoglycoside phosphotransferases that confer resistance to gentamicin (CHQ90_18865, CHQ90_15195, and A8A08_04525) and streptomycin...”

Q2FD58 streptomycin 3''-adenylyltransferase (EC 2.7.7.47) from Acinetobacter baumannii (see paper)
Aligns to 146:249 / 262 (39.7%), covers 95.1% of PF13427, 67.0 bits

A8A08_02265, CHQ90_08400 ANT(3'')-II family aminoglycoside nucleotidyltransferase from Acinetobacter baumannii
Aligns to 146:249 / 262 (39.7%), covers 95.1% of PF13427, 65.9 bits

• Genotypic and Phenotypic Characterization of Novel Sequence Types of Carbapenem-Resistant Acinetobacter baumannii, With Heterogeneous Resistance Determinants and Targeted Variations in Efflux Operons
  Bharathi, Frontiers in microbiology 2021
  • “...to streptomycin, spectinomycin, was detected in A. baumannii strains DR1 CHQ89_16705, DR2 CHQ90_08400, and AB067 A8A08_02265 respectively, and these genes were found in the genomic island region. The aminoglycoside phosphotransferases that confer resistance to gentamicin (CHQ90_18865, CHQ90_15195, and A8A08_04525) and streptomycin (CHQ90_15190 and A8A08_04530) were also...”
  • “...that confers resistance to streptomycin, spectinomycin, was detected in A. baumannii strains DR1 CHQ89_16705, DR2 CHQ90_08400, and AB067 A8A08_02265 respectively, and these genes were found in the genomic island region. The aminoglycoside phosphotransferases that confer resistance to gentamicin (CHQ90_18865, CHQ90_15195, and A8A08_04525) and streptomycin (CHQ90_15190 and...”

lpp1089 hypothetical protein from Legionella pneumophila str. Paris
Aligns to 489:598 / 601 (18.3%), covers 94.2% of PF13427, 54.0 bits

• Comparative Genomics Reveal That Host-Innate Immune Responses Influence the Clinical Prevalence of Legionella pneumophila Serogroups
  Khan, PloS one 2013
  • “...WecA O-antigen initiating glycosyl transferase Lp6_761 HP 48 lpg0788 HP Lp6_762 GCN5-related N -acetyltransferase 29 lpp1089 Streptomycin 3''-adenylyltransferase Lp6_763 PseG Pseudaminic acid biosynthesis-associated protein Lp6_764 Glutamate-1-semialdehyde 2,1-aminomutase 37 lpg0467 LasB Zinc metalloprotease Lp6_765 GlmU1 Acylneuraminate cytidylyltransferase 31 lpg1919 KdsB 3-deoxy-manno-octulosonate cytidylyltransferase Lp6_766 Acetyltransferase 50 lpg2848 Lp6_767...”

F1721_06460 nucleotidyltransferase domain-containing protein from Saccharopolyspora hirsuta
Aligns to 163:251 / 271 (32.8%), covers 60.2% of PF13427, 32.1 bits

• Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666^T Strain
  Lobastova, International journal of molecular sciences 2022
  • “...F1721_27890 ); metal ions resistance ( F1721_28715 ); and a role of carrier proteins ( F1721_06460 ). The F1721_15555 protein could be mentioned as potentially involved in metabolism of steroids ( Table 2 ). A 2.9-fold increase was observed in expression of the F1721_32655 gene in...”

Tfu_2432 hypothetical protein from Thermobifida fusca YX
Aligns to 180:256 / 269 (28.6%), covers 49.5% of PF13427, 30.6 bits

• Identification and manipulation of the caprazamycin gene cluster lead to new simplified liponucleoside antibiotics and give insights into the biosynthetic pathway
  Kaysser, The Journal of biological chemistry 2009
  • “...olivaceus elmM1, S. olivaceus elmGT, S. olivaceus Tfu_2432, Thermobifida fusca SchA32, Streptomyces sp. SCC 2136 SchA33, Streptomyces sp. SCC 2136 CAH10130...”

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