Family Search for PF13689 (DUF4154)

April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.

PF13689 hits 14 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

YFIR_PSEAE / Q9I4L4 Negative regulator YfiR from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 6 papers)
Aligns to 45:185 / 190 (74.2%), covers 97.9% of PF13689, 114.3 bits

• function: Negatively regulates the activity of the diguanylate cyclase TpbB/YfiN, leading to decreased c-di-GMP production (PubMed:20300602). Inhibits TpbB/YfiN allosterically, through a hydrophobic interaction between the C-terminus of YfiR and a conserved region of the periplasmic PAS domain of TpbB/YfiN (PubMed:22719254). Under reducing conditions, may also act as an YfiB-independent sensing device that is able to activate TpbB/YfiN in response to the redox status of the periplasm (PubMed:22719254).
  function: Part of the YfiB-TpbB-YfiR (or yfiBNR) system, encoding a tripartite signaling module that modulates intracellular c-di-GMP levels (PubMed:20300602, PubMed:22719254). The system is a key regulator of the small colony variant (SCV) phenotype, and plays an important role in biofilm formation and in vivo persistence (PubMed:20300602). The c-di-GMP produced by TpbB/YfiN stimulates the production of the Pel and Psl exopolysaccharides, which promotes surface attachment, generates an SCV phenotype and confers resistance against phagocytosis (PubMed:20300602).
  subunit: Homodimer (PubMed:25849887, PubMed:26593397, PubMed:27113583). Interacts with TpbB/YfiN (PubMed:22719254). Interacts with YfiB (PubMed:26593397, PubMed:27113583, PubMed:28870806). The YfiB-YfiR complex is a 2:2 heterotetramer (PubMed:27113583).
  disruption phenotype: Deletion of the gene results in the generation of a small colony variant (SCV) morphology in PAO1 (PubMed:20300602). Mutant exhibits slow growth, reduced motility, a highly aggregative and wrinkled colony morphology, strong attachment to surfaces and strong, exopolysaccharide-dependent resistance to macrophage phagocytosis (PubMed:20300602). The deletion mutant, although suffering from a tremendous growth disadvantage, shows characteristic persistence behavior under antibiotic selection and during prolonged infection in vivo (PubMed:20300602).

YfiR / b2603 DUF4154 domain-containing protein YfiR from Escherichia coli K-12 substr. MG1655 (see 5 papers)
YFIR_ECOLI / P64548 Protein YfiR from Escherichia coli (strain K12) (see paper)
Aligns to 29:167 / 172 (80.8%), covers 97.2% of PF13689, 109.3 bits

• function: Repressor of the cell division arrest function of DgcN. Prevents DgcN relocation to the midcell.

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