Family Search for PF14393 (DUF4422)

PF14393 hits 17 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

GW747_RS07680 DUF4422 domain-containing protein from Limosilactobacillus fermentum
Aligns to 4:220 / 253 (85.8%), covers 99.1% of PF14393, 309.1 bits

• Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro
  Butorac, Microbial cell factories 2021
  • “...found. The priming glycosyltransferase, epsE (GW747_RS07685), corresponding to the undecaprenyl galactose phosphotransferase, as well as GW747_RS07680 gene, that encodes a protein containing a domain DUF4422 and is related with the incorporation of galactofuranose into polysaccharides, were also detected within the cluster. Detection of the genes involved...”

LBA1732 galactosyl transferase from Lactobacillus acidophilus NCFM
Aligns to 4:221 / 257 (84.8%), covers 99.5% of PF14393, 306.0 bits

• Effects of galactosyltransferase on EPS biosynthesis and freeze-drying resistance of Lactobacillus acidophilus NCFM
  Kong, Food chemistry. Molecular sciences 2022
  • “...To explore this mechanism, epsF (National Center for Biotechnology Information GenBank (NCBI) accession No. CP000033.3: LBA1732, https://www.ncbi.nlm.nih.gov/nuccore/CP000033.3 ), which encodes GalT, was cloned and overexpressed in L. acidophilus NCFM to evaluate the influence of the changes in GalT activity on EPS biosynthesis and freeze-drying survival rate....”

LSL_1574 DUF4422 domain-containing protein from Ligilactobacillus salivarius UCC118
LSL_1574 Glycosyltransferase from Lactobacillus salivarius subsp. salivarius UCC118
Aligns to 6:226 / 270 (81.9%), covers 99.1% of PF14393, 301.2 bits

• Functional and Genomic Characterization of Ligilactobacillus salivarius TUCO-L2 Isolated From Lama glama Milk: A Promising Immunobiotic Strain to Combat Infections
  Quilodrán-Vega, Frontiers in microbiology 2020
  • “...EPS clusters from L. salivarius UCC118 (EPS1: from LSL_077 to LSL_097; EPS2: from LSL_1547 to LSL_1574) and JCM1046 (EPS3: from LSJ_1603c to LSJ_1633c) were used for comparison. (B) Phylogenetic tree was constructed by using the sequences of twelve conserved EPS genes shared by the strains isolated...”
• Selection of Immunobiotic Ligilactobacillus salivarius Strains from the Intestinal Tract of Wakame-Fed Pigs: Functional and Genomic Studies
  Zhou, Microorganisms 2020
  • “...FFIG23 and FFIG63 strains ( Figure 4 , Table S6 ). In addition, the glycosyltransferase LSL_1574 from the EPS cluster 2 was not found in the FFIG63 genome. Similar results were obtained when the EPS cluster 3 was used to analyze the FFIG genomes. These results...”
• Phylogenomics and comparative genomics of Lactobacillus salivarius, a mammalian gut commensal
  Harris, Microbial genomics 2017
  • “...of EPS cluster 2 are shared by all the strains ( Fig. 9 ; from LSL_1574 to LSL_1569 and from LSL_1551 to LSL_1547). However, variations exist in the middle of EPS cluster 2 and six groups were identified as described in Fig. S2. Group 1 contained...”

lp_1180 glycosyltransferase from Lactobacillus plantarum WCFS1
lp_1180 DUF4422 domain-containing protein from Lactiplantibacillus plantarum WCFS1
Aligns to 4:221 / 258 (84.5%), covers 99.5% of PF14393, 296.5 bits

• Molecular Responses of Lactobacilli to Plant Phenolic Compounds: A Comparative Review of the Mechanisms Involved
  López, Antioxidants (Basel, Switzerland) 2021
  • “...cps4C : polysaccharide biosynthesis gene cluster 4 proteins RSV [ 27 ] Reduced SPS levels lp_1180; lp_1184-lp_1185 cps1D; cps1H-I: polysaccharide biosynthesis gene cluster 1 proteins OLE [ 28 ] Reduced SPS levels lp_1197- lp_1198 cps2AB : polysaccharide biosynthesis gene cluster 2 proteins OLE Reduced SPS levels...”
• Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts
  Santamaría, Frontiers in microbiology 2019
  • “...different surface polysaccharide gene clusters contained in the L. plantarum genome, cps1 ( cps1D or lp_1180 , cps1H or lp_1184 , cps1I or lp_1185 ), cps2 ( cps2A or lp_1197 , cps2B or lp_1198 , cps2E or lp_1201 , cps2F or lp_1202 , cps2G or lp_1203...”
• Exploring Lactobacillus plantarum genome diversity by using microarrays
  Molenaar, Journal of bacteriology 2005
  • “...from a non-ribosomal protein biosynthesis cluster lp_1180, lp_1186, lp_1190, lp_1202 .......Some genes from an exopolysaccharide biosynthesis cluster...”

GI|68643903 capsular biosynthesis protein from Streptococcus pneumoniae (see paper)
Aligns to 5:226 / 263 (84.4%), covers 99.5% of PF14393, 294.9 bits

lp_1227 polysaccharide biosynthesis protein from Lactobacillus plantarum WCFS1
Aligns to 4:220 / 258 (84.1%), covers 100.0% of PF14393, 294.9 bits

• An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence
  Sturme, Journal of bacteriology 2005
  • “...to lp_1190), cps2 (lp_1197 to lp_1211), cps3 (lp_1215 to lp_1227), and cps4 (lp_2099 to lp_2108) (27). The number of ORFs showing altered gene expression in the...”

BEN15_RS08540, DIS31_02560, T303_06385 DUF4422 domain-containing protein from Streptococcus thermophilus ASCC 1275
Aligns to 5:225 / 259 (85.3%), covers 99.1% of PF14393, 284.2 bits

• The N-terminal domain of rhamnosyltransferase EpsF influences exopolysaccharide chain length determination in Streptococcus thermophilus 05-34
  Wang, PeerJ 2020
  • “...inactivated gene. The central region of the eps cluster encodes an EPS biosynthesis protein EpsG (DIS31_02560) with unknown function, and several glycosyltransferases. Among them, EpsE (DIS31_02540) was 100% identical to the priming glycosyltransferase of S. thermophilus NCFB 2393, which was shown to transfer glucosyl 1-phosphate to...”
• Functional Genomic Analyses of Exopolysaccharide-Producing Streptococcus thermophilus ASCC 1275 in Response to Milk Fermentation Conditions
  Wu, Frontiers in microbiology 2019
  • “...(detected proteins) highlighted in red indicates up-DEPs. As for EPS biosynthesis, only five proteins (T303_06336, T303_06385, T303_06400, T303_06410 and T303_06415) out of 20 EPS assembly associated proteins were confidently detected by iTRAQ-based method ( Supplementary Table S2 ); only EpsB (T303_06415) was identified as the down-regulated...”
• Comparative mRNA-Seq Analysis Reveals the Improved EPS Production Machinery in Streptococcus thermophilus ASCC 1275 During Optimized Milk Fermentation
  Wu, Frontiers in microbiology 2018
  • “...release EPS. As presented in Figure 4 , a similar expression pattern (from T303_06320 to T303_06385) was observed in Cd2, Cd3, and Cd4 compared to that in Cd1; this suggests that temperature increase (Cd3 vs. Cd2) and WPI supplementation (Cd4 vs. Cd2) did not alter the...”
• Complete Genome Sequence of Streptococcus thermophilus KLDS 3.1003, A Strain with High Antimicrobial Potential against Foodborne and Vaginal Pathogens
  Evivie, Frontiers in microbiology 2017
  • “...S. thermophilus KLDS3.1003 genome also contains epsA (BEN15_RS08575), epsB (BEN15_RS08570), epsE (BEN15_RS08555), epsF (BEN15_RS08550), epsG (BEN15_RS08540), epsH (BEN15_08530), epsI (BEN15_08530), eps J (BEN15_RS08530), eps K (BEN15_RS08515), epsL (BEN15_RS08510), epsM (BEN15_RS08505), epsN (BEN15_RS08500), epsO (BEN15_RS0190), epsP (BEN15_RS08475), epsQ (BEN15_RS00260, BEN15_RS00265), and transposase (BEN15_RS08545). Some of these have...”

D805_0356 DUF4422 domain-containing protein from Bifidobacterium thermophilum RBL67
Aligns to 20:244 / 282 (79.8%), covers 98.2% of PF14393, 273.1 bits

• Bifidobacterium thermophilum RBL67 impacts on growth and virulence gene expression of Salmonella enterica subsp. enterica serovar Typhimurium
  Tanner, BMC microbiology 2016
  • “...glycosyl transferase, group 1 family protein 2.07 4.79 3.0E-10 D805_0355 hypothetical protein 1.34 6.14 4.5E-06 D805_0356 Glycosyltransferase (EC 2.4.1.-) 2.14 4.96 1.5E-15 D805_0512 hypothetical protein 2.01 2.39 0.03026 D805_0524 D-lactate dehydrogenase (EC 1.1.1.28) 2.85 3.15 0.00049 D805_0525 Aspartate aminotransferase (EC 2.6.1.1) 1.91 3.11 0.01352 D805_0652 Oligopeptide...”

AO080_09105 DUF4422 domain-containing protein from Weissella cibaria
Aligns to 2:220 / 257 (85.2%), covers 99.5% of PF14393, 266.3 bits

• Next-Generation Sequencing for Whole-Genome Characterization of Weissella cibaria UTNGt21O Strain Originated From Wild Solanum quitoense Lam. Fruits: An Atlas of Metabolites With Biotechnological Significance
  Tenea, Frontiers in microbiology 2021
  • “...and chaperones. A hypothetical protein, UTNGt21O Query ID: 00795, was classified with KEGG ID: wcb: AO080_09105 exopolysaccharide biosynthesis protein, but no pathway was associated with this protein. Complementary in vitro analysis indicated no production of exopolysaccharide from glucose (40%) or sucrose (40%) by the UTNGt21O strain...”

LGG_02048 lipo/exopolysaccharide biosynthesis glycosyltransferase / EpsF/EpsIID from Lactobacillus rhamnosus GG
Aligns to 4:222 / 265 (82.6%), covers 99.5% of PF14393, 258.7 bits

• A network-based approach to identify substrate classes of bacterial glycosyltransferases
  Sánchez-Rodríguez, BMC genomics 2014
  • “...[ 37 , 44 ], our analysis only missed welJ , annotated as alpha-1,3-galactosyltransferase ( LGG_02048 ), as this gene was not predicted as a GT in our analysis. This gene does not appear to contain any signatures of the currently known HMMs for GTs and...”

LGG_02048 DUF4422 domain-containing protein from Lacticaseibacillus rhamnosus GG
Aligns to 7:225 / 268 (81.7%), covers 99.5% of PF14393, 258.7 bits

• A network-based approach to identify substrate classes of bacterial glycosyltransferases
  Sánchez-Rodríguez, BMC genomics 2014
  • “...[ 37 , 44 ], our analysis only missed welJ , annotated as alpha-1,3-galactosyltransferase ( LGG_02048 ), as this gene was not predicted as a GT in our analysis. This gene does not appear to contain any signatures of the currently known HMMs for GTs and...”

Q1G8E9 EpsIH, Putative glycosyltransferase from Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14)
Aligns to 11:222 / 267 (79.4%), covers 99.1% of PF14393, 237.8 bits

• Effects of ccpA gene deficiency in Lactobacillus delbrueckii subsp. bulgaricus under aerobic conditions as assessed by proteomic analysis
  Zhang, Microbial cell factories 2020
  • “...transport and metabolism A0A061BX52 EpsIIH, glycosyltransferase 42.16 18.01 1.802 Q1G8F3 EpsIM, glycosyltransferase 40.55 24.17 1.744 Q1G8E9 EpsIH, glycosyltransferase 31.48 28.09 1.624 Q1G7Z6 Pyruvate oxidase 67.67 64.94 1.592 Q1G8E8 EpsIG, glycosyltransferase 19.19 38.92 1.529 Q1GAY6 Acetate kinase 43.16 46.08 1.512 Q71HT0 Phosphopyruvate hydratase 15.15 82.19 1.520 A0A061BLT3...”

6u4bA / Q48484 Wbbm bifunctional glycosytransferase apo structure (see paper)
Aligns to 4:245 / 570 (42.5%), covers 99.1% of PF14393, 236.9 bits

• Ligand: magnesium ion (6u4bA)

AAC98416.1 LPS [galactan I] α-1,3-galactopyranosyltransferase (WbbM;RfbC) (EC 2.4.1.-) (see protein)
Aligns to 10:251 / 635 (38.1%), covers 99.1% of PF14393, 236.8 bits

Bbr_0440 DUF4422 domain-containing protein from Bifidobacterium breve UCC2003
Aligns to 4:227 / 276 (81.2%), covers 99.5% of PF14393, 235.6 bits

• Comparative genomics and genotype-phenotype associations in Bifidobacterium breve
  Bottacini, Scientific reports 2018
  • “...al . 55 Bbr_0439 Capsular polysaccharide biosynthesis protein X PF05704 Fanning et al . 55 Bbr_0440 Polysaccharide biosynthesis protein X PF14393 Fanning et al . 55 Bbr_0447 Conserved hypothetical protein PF04230 Fanning et al . 55 Bbr_0449 Hypothetical membrane spanning protein No hit Fanning et al...”

KP1_RS17240 DUF4422 domain-containing protein from Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
KP1_3696 putative glycosyltransferase from Klebsiella pneumoniae NTUH-K2044
Aligns to 6:247 / 631 (38.4%), covers 99.1% of PF14393, 234.5 bits

• Within-Host Genotypic and Phenotypic Diversity of Contemporaneous Carbapenem-Resistant Klebsiella pneumoniae from Blood Cultures of Patients with Bacteremia
  Cheng, mBio 2022
  • “...bold. Chr, chromosome; KP1_RS17220, glycosyltransferase, LPS; KP1_RS17225, glycosyltransferase family 4 protein, LPS; KP1_RS17230, glycosyltransferase, LPS; KP1_RS17240, DUF4422 domain-containing protein, LPS; KP1_RS17355, phosphatase PAP2 family protein, capsule. Download TableS4, DOCX file, 0.02 MB . Copyright 2022 Cheng et al. 2022 Cheng et al. https://creativecommons.org/licenses/by/4.0/ This content is...”
• D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design
  Hsieh, Frontiers in microbiology 2014
  • “...function Locus tag (NTUH-K2044 ORF) Gene size (bp) Tn insertion point 3-4A wbbM Putative glycosyltransferase KP1_3696 1908 1207 4-5B wbbO Bifunctional galactosyltransferase KP1_3693 1143 70 7-6D wbbM putative glycosyltransferase KP1_3696 1908 1647 9-3E wbbN Putative glycosyltransferase KP1_3694 894 293 9-10D wbbM Putative glycosyltransferase KP1_3696 1908 1635...”
  • “...component KP1_3697 741 149 25-3F wbbN Putative glycosyltransferase KP1_3694 894 280 25-8H wbbM Putative glycosyltransferase KP1_3696 1908 1056 26-8B glf Putative UDP-galactopyranose mutase KP1_3695 1155 954 The target gene or bacterial strains studied in this study are mentioned in bold . Genetic analysis of wbbY and...”

CJM1_1382 DUF4422 domain-containing protein from Campylobacter jejuni subsp. jejuni M1
Aligns to 382:631 / 978 (25.6%), covers 98.2% of PF14393, 187.4 bits

• Genomic characterization of Campylobacter jejuni strain M1
  Friis, PloS one 2010
  • “...CJM1_0138, CJM1_0278 , CJM1_0414, CJM1_0728, CJM1_0745 , CJM1_1118, CJM1_1119, CJM1_1120, CJM1_1372, CJM1_1373, CJM1_1375 , CJM1_1381, CJM1_1382 C8J_0035, C8J_0036, C8J_0063, C8J_0132, C8J_0133 , C8J_0154, C8J_0265, C8J_0266, C8J_0267, C8J_0272, C8J_0273, C8J_0274 , C8J_0275, C8J_0321, C8J_0529, C8J_0651, C8J_0776, C8J_0852, C8J_0918, C8J_1080, C8J_1081, C8J_1083, C8J_1329, C8J_1330, C8J_0131, C8J_1332, C8J_1334, C8J_1335,...”

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