Family Search for PF14574 (RACo_C_ter)

April 2024: GapMind for amino acid biosynthesis has been updated to include newly-discovered enzymes and comparative genomics predictions (see details)

Running HMMer for PF14574

PF14574 hits 40 sequences in PaperBLAST's database above the trusted cutoff. Showing hits to curated sequences only. Or see all hits or try another family.

PGA1_c15200 ATP-dependent reduction of co(II)balamin (RamA-like) (EC:2.1.1.13) from Phaeobacter inhibens BS107
Aligns to 395:668 / 698 (39.3%), covers 98.5% of PF14574, 292.0 bits

• mutant phenotype: Apparently required for the reactivation of vitamin B12. Distantly related to RamA (see PMID: 19043046) (auxotroph)

B8R2M5 [Co(II) methylated amine-specific corrinoid protein] reductase (EC 1.16.99.1) from Acetobacterium dehalogenans (see paper)
Aligns to 339:598 / 598 (43.5%), covers 96.9% of PF14574, 251.6 bits

RAMQ_EUBLI / P0DX10 Corrinoid activation enzyme RamQ from Eubacterium limosum (see 2 papers)
Aligns to 334:592 / 593 (43.7%), covers 95.0% of PF14574, 245.8 bits

• function: Involved in the degradation of the quaternary amines L- proline betaine and L-carnitine (PubMed:31341018, PubMed:32571881). Component of a corrinoid-dependent methyltransferase system that transfers a methyl group from L-proline betaine or L-carnitine to tetrahydrofolate (THF), forming methyl-THF, a key intermediate in the Wood-Ljungdahl acetogenesis pathway (PubMed:31341018, PubMed:32571881). RamQ is not required for the methyl transfer, but it stimulates reduction of reconstituted MtqC from the Co(II) state to the Co(I) state in vitro (PubMed:31341018). It also stimulates the rate of THF methylation (PubMed:32571881).
  cofactor: [2Fe-2S] cluster (Binds 1 2Fe-2S cluster.)

ramA / B8Y445 [Co(II) methylated amines-specific corrinoid protein] reductase (EC 1.16.99.1) from Methanosarcina barkeri (see 2 papers)
B8Y445 [Co(II) methylated amine-specific corrinoid protein] reductase (EC 1.16.99.1) from Methanosarcina barkeri (see paper)
Aligns to 168:427 / 540 (48.1%), covers 98.5% of PF14574, 209.8 bits

DvMF_1398 ATP-dependent reduction of co(II)balamin (RamA-like) from Desulfovibrio vulgaris Miyazaki F
Aligns to 434:685 / 685 (36.8%), covers 93.9% of PF14574, 143.7 bits

• mutant phenotype: Cofit with the B12-dependent methionine synthase (DvMF_0476), which lacks a standard domain for the reactivation of vitamin B12.

DVU0908 ATP-dependent reduction of co(II)balamin from Desulfovibrio vulgaris Hildenborough JW710
Aligns to 296:542 / 543 (45.5%), covers 94.3% of PF14574, 139.1 bits

• mutant phenotype: Important for fitness in most defined media. Semi-automated annotation based on the auxotrophic phenotype and a hit to HMM PF14574.

Or search for genetic data about PF14574 in the Fitness Browser