Family Search for PF14904 (FAM86)

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PF14904 hits 7 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

8fzbA / Q96G04 Crystal structure of human fam86a (see paper)
Aligns to 1:94 / 318 (29.6%), covers 97.9% of PF14904, 154.3 bits

• Ligand: s-adenosyl-l-homocysteine (8fzbA)

EF2KT_HUMAN / Q96G04 Protein-lysine N-methyltransferase EEF2KMT; eEF2-lysine methyltransferase; eEF2-KMT; EC 2.1.1.- from Homo sapiens (Human) (see 2 papers)
Aligns to 6:99 / 330 (28.5%), covers 97.9% of PF14904, 154.2 bits

• function: Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.
  catalytic activity: L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L- homocysteine (RHEA:54192)
  subunit: Interacts with FAM86B2 and FAM86C1P.
• Human seven-β-strand (METTL) methyltransferases - conquering the universe of protein lysine methylation
  Falnes, The Journal of biological chemistry 2023
  • “...METTL13 EEF1AKNMT, FEAT Q8N6R0 eEF1A d 55 me2 ( 32 , 34 ) eEF2-KMT FAM86A Q96G04 eEF2 525 me3 ( 27 , 61 , 62 ) METTL21C Q5VZV1 AARS1 943 me1,2,3 ( 44 ) ETF-KMT METTL20 Q8IXQ9 ETF 200, 203 me1,2,3 ( 36 , 41 )...”
• The FAM86 domain of FAM86A confers substrate specificity to promote EEF2-Lys525 methylation.
  Francis, The Journal of biological chemistry 2023
  • “...study. For transient transfection, the following genes were cloned into pQCXIH-CMV/TO-DEST (Addgene): FAM86A (UniProt ID: Q96G04 ) including all FAM86A mutants, FAM86B1 (Uniprot ID: Q8N7N1 ), FAM86B2 (Uniprot ID: P0C5J1 ). For bacterial protein expression and purification, FAM86A and its mutants were cloned into pGEX-6P-1 (Addgene)....”
• Chemical Proteomic Profiling of Human Methyltransferases.
  Horning, Journal of the American Chemical Society 2016
  • “...Q9H8H3 METTL7 Uncharacterized 20 20 Q96LA8 PRMT6 Protein 20 20 Q9H1A3 METTL9 Uncharacterized 20 20 Q96G04 EEF2KM Protein 20 20 Q7Z4G4 TRMT1 Uncharacterized 20 20 Q5JPI9 METTL1 Protein 20 20 Non-MTs Q8WXB METTL2 Protein 20 20 Q2M2I8 AAK1 Protein (kinase) 20 16 Q9NRG SMYD2 Protein 20...”
• The activity of a yeast Family 16 methyltransferase, Efm2, is affected by a conserved tryptophan and its N-terminal region.
  Hamey, FEBS open bio 2016
  • “...29 , 30 METTL22 Human KIN17K135 Tri Q9BUU2 29 , 31 eEF2KMT Human eEF2K525 Tri Q96G04 21 CaMKMT Human CalmodulinK116 Tri Q7Z624 27 ETFBKMT Human ETFBK200/K203 Tri/Tri Q8IXQ9 32 , 33 METTL18 Human O95568 METTL21B Human Q96AZ1 METTL21C Human Q5VZV1 METTL23 Human Q86XA0 John Wiley &...”

F86B2_HUMAN / P0C5J1 Putative protein N-methyltransferase FAM86B2; EC 2.1.1.- from Homo sapiens (Human) (see paper)
Aligns to 6:99 / 330 (28.5%), covers 97.9% of PF14904, 150.3 bits

• subunit: Interacts with EEF2KMT.
• The FAM86 domain of FAM86A confers substrate specificity to promote EEF2-Lys525 methylation.
  Francis, The Journal of biological chemistry 2023
  • “...ID: Q96G04 ) including all FAM86A mutants, FAM86B1 (Uniprot ID: Q8N7N1 ), FAM86B2 (Uniprot ID: P0C5J1 ). For bacterial protein expression and purification, FAM86A and its mutants were cloned into pGEX-6P-1 (Addgene). For protein expression and purification from human cells, EEF2 (Uniprot ID: P13639 ) and...”
• Chemical Proteomic Profiling of Human Methyltransferases.
  Horning, Journal of the American Chemical Society 2016
  • “...Q9BV86 NTMT1 Other 20 20 Q8N7N1 FAM86 Uncharacterized 20 20 P22061 PCMT1 Other 20 20 P0C5J1 FAM86 Uncharacterized 20 20 Q86X55 CARM1 Protein 15 20 Q8N6R0 METTL1 Uncharacterized 20 20 Q99873 PRMT1 Protein 20 20 Q9NRN9 METTL5 Uncharacterized 20 12 O60678 PRMT3 Prortein 15 20 Q9H8H3...”

Q8N7N1 Putative protein N-methyltransferase FAM86B1 from Homo sapiens
Aligns to 6:88 / 296 (28.0%), covers 79.8% of PF14904, 116.2 bits

• The FAM86 domain of FAM86A confers substrate specificity to promote EEF2-Lys525 methylation.
  Francis, The Journal of biological chemistry 2023
  • “...into pQCXIH-CMV/TO-DEST (Addgene): FAM86A (UniProt ID: Q96G04 ) including all FAM86A mutants, FAM86B1 (Uniprot ID: Q8N7N1 ), FAM86B2 (Uniprot ID: P0C5J1 ). For bacterial protein expression and purification, FAM86A and its mutants were cloned into pGEX-6P-1 (Addgene). For protein expression and purification from human cells, EEF2...”
• Chemical Proteomic Profiling of Human Methyltransferases.
  Horning, Journal of the American Chemical Society 2016
  • “...Q9UIC8 LCMT1 Other 20 20 O60344 ECE2 Uncharacterized 20 20 Q9BV86 NTMT1 Other 20 20 Q8N7N1 FAM86 Uncharacterized 20 20 P22061 PCMT1 Other 20 20 P0C5J1 FAM86 Uncharacterized 20 20 Q86X55 CARM1 Protein 15 20 Q8N6R0 METTL1 Uncharacterized 20 20 Q99873 PRMT1 Protein 20 20 Q9NRN9...”

XP_005255214 protein-lysine N-methyltransferase EEF2KMT isoform X1 from Homo sapiens
2 alignments in 6:72 / 303 (22.1%), covering up to 50.0% of PF14904, 72.6 bits

• The FAM86 domain of FAM86A confers substrate specificity to promote EEF2-Lys525 methylation.
  Francis, The Journal of biological chemistry 2023
  • GeneRIF: The FAM86 domain of FAM86A confers substrate specificity to promote EEF2-Lys525 methylation.
• Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2).
  Davydova, The Journal of biological chemistry 2014
  • GeneRIF: Previously uncharacterized lysine-specific methyltransferase FAM86A and Yjr129c introduce a functionally important lysine methylation in eEF2.

F86C1_HUMAN / Q9NVL1 Putative protein FAM86C1P; Protein FAM86C; EC 2.1.1.- from Homo sapiens (Human) (see paper)
Aligns to 6:53 / 165 (29.1%), covers 48.9% of PF14904, 66.0 bits

• subunit: Interacts with EEF2KMT.

R4GN25 Family with sequence similarity 86 member B1 from Homo sapiens
Aligns to 6:35 / 108 (27.8%), covers 27.7% of PF14904, 26.2 bits

• Host neuronal PRSS3 interacts with enterovirus A71 3A protein and its role in viral replication
  Rattanakomol, Scientific reports 2022
  • “...sapiens ] 27.9 O14818 1 48 Putative protein N-methyltransferase FAM86B1 [ Homo sapiens ] 48.3 R4GN25 2 49 Figure 2 Multialignment of amino acid sequences of PRSS1 and four PRSS3 transcript variants (PRSS3 variants 14; PRSS3-v1 to PRSS3-v4 ) by DNAMAN program. Highlight homology level: blue=100%,...”

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