Family Search for PF14990 (DUF4516)
April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.
PF14990 hits 6 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
UQCC6_MOUSE / Q8BTC1 Ubiquinol-cytochrome c reductase complex assembly factor 6; Protein BRAWNIN from Mus musculus (Mouse) (see 2 papers)
Aligns to 1:46 / 67 (68.7%), covers 100.0% of PF14990, 89.9 bits
- function: Required for the assembly and stability of the mitochondrial ubiquinol-cytochrome c reductase complex (complex III or cytochrome b- c1 complex), a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain (ETC) which drives oxidative phosphorylation (PubMed:32161263, PubMed:35977508). Mediates early complex III biogenesis (PubMed:35977508). Participates in regulating the levels of electron transport chain proteins, and therefore energy supply, in response to changes in energy demand (PubMed:35977508). Also required for cytochrome c oxidase complex (complex IV) assembly (By similarity).
subunit: Interacts with UQCRC1 (PubMed:32161263). Interacts with UQCRQ (PubMed:32161263). Interacts with UQCC5 (By similarity). Forms a complex, named COMB/coordinator of mitochondrial CYTB biogenesis, composed of UQCC1, UQCC2, UQCC4, UQCC5 and UQCC6; stabilizes nascent cytochrome b/MT-CYB and promotes its membrane insertion (PubMed:35977508). Forms a complex, named COMA, composed of UQCC1, UQCC2 and UQCC4; activates MT-CYB translation (PubMed:35977508). Forms a complex, named COMC, composed of UQCC1, UQCC2; UQCC3 and UQCC4; mediates MT-CYB hemylation and association with the first nuclear- encoded complex III subunit UQCRQ (PubMed:35977508). Interacts with MT- CYB (PubMed:35977508).
disruption phenotype: Uqcc6 deficiency leads to complete loss of mitochondrial respiratory chain complex III, resulting in growth retardation and a mild increase in blood lactate levels and significantly decreased exercise tolerance. The subunits of mitochondrial respiratory chain complex I, II, IV, and V are not affected. - Microproteins: Overlooked regulators of physiology and disease.
Hassel, iScience 2023 - “...NR NR BRAWNIN Electron transport chain complex III assembly factor UQCC6 71 Q69YU5 Uqcc6 67 Q8BTC1 CASIMO1 Regulator of cytoskeletal organization, cell migration and proliferation SMIM22 83 K7EJ46 Smim22 86 V9GXA9 CIP2A-BP Negative regulator of PI3K/AKT/NFkB pathway through interaction with CIP2A LINC00665 52 NR NR NR...”
UQCC6_DANRE / E7EXZ6 Ubiquinol-cytochrome-c reductase complex assembly factor 6; Protein brawnin from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
NP_001129045 uncharacterized protein C12orf73 homolog from Danio rerio
Aligns to 1:46 / 65 (70.8%), covers 100.0% of PF14990, 89.4 bits
- function: Required for the assembly and stability of the mitochondrial ubiquinol-cytochrome c reductase complex (complex III (CIII) or cytochrome b-c1 complex), a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain (ETC) which drives oxidative phosphorylation (PubMed:32161263). Mediates early complex III biogenesis. Participates in regulating the levels of electron transport chain proteins, and therefore energy supply, in response to changes in energy demand (By similarity). Also required for cytochrome c oxidase complex (complex IV) assembly (By similarity).
disruption phenotype: A complete loss of mitochondrial respiratory chain complex III resulting in severe growth retardation, lactic acidosis and early death. - Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly.
Zhang, Nature communications 2020 - GeneRIF: Study demonstrate that Brawnin, a 71 a.a. peptide encoded by C12orf73, is essential for respiratory chain complex III (CIII) assembly. In zebrafish, Brawnin deletion causes complete CIII loss, resulting in severe growth retardation, lactic acidosis and early death. Findings demonstrate that BRAWNIN is essential for vertebrate oxidative phosphorylation.
UQCC6_HUMAN / Q69YU5 Ubiquinol-cytochrome c reductase complex assembly factor 6; Protein BRAWNIN from Homo sapiens (Human) (see 2 papers)
XP_016875406 ubiquinol-cytochrome-c reductase complex assembly factor 6 isoform X2 from Homo sapiens
Aligns to 1:46 / 71 (64.8%), covers 97.8% of PF14990, 79.3 bits
- function: Required for the assembly and stability of the mitochondrial ubiquinol-cytochrome c reductase complex (complex III (CIII) or cytochrome b-c1 complex), a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain (ETC) which drives oxidative phosphorylation (PubMed:32161263). Mediates early complex III biogenesis (By similarity). Participates in regulating the levels of electron transport chain proteins, and therefore energy supply, in response to changes in energy demand (By similarity). Also required for cytochrome c oxidase complex (complex IV) assembly (PubMed:34969438).
subunit: Interacts with UQCRC1 (PubMed:32161263). Interacts with UQCRQ (By similarity). Interacts with UQCC5 (PubMed:34969438). Forms a complex, named COMB/coordinator of mitochondrial CYTB biogenesis, composed of UQCC1, UQCC2, UQCC4, UQCC5 and UQCC6; stabilizes nascent cytochrome b/MT-CYB and promotes its membrane insertion. Forms a complex, named COMA, composed of UQCC1, UQCC2 and UQCC4; activates MT- CYB translation. Forms a complex, named COMC, composed of UQCC1, UQCC2; UQCC3 and UQCC4; mediates MT-CYB hemylation and association with the first nuclear-encoded complex III subunit UQCRQ (By similarity). Interacts with MT-CYB (By similarity). - Knockdown of BRAWNIN minimally affect mitochondrial complex III assembly in human cells.
Wang, Biochimica et biophysica acta. Molecular cell research 2024 (PubMed)- GeneRIF: Knockdown of BRAWNIN minimally affect mitochondrial complex III assembly in human cells.
- Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly.
Zhang, Nature communications 2020 - GeneRIF: Study demonstrate that BRAWNIN, a 71 a.a. peptide encoded by C12orf73, is essential for respiratory chain complex III (CIII) assembly. In human cells, BRAWNIN is induced by the energy-sensing AMPK pathway, and its depletion impairs mitochondrial ATP production. Findings demonstrate that BRAWNIN is essential for vertebrate oxidative phosphorylation.
- Microproteins: Overlooked regulators of physiology and disease.
Hassel, iScience 2023 - “...94 NR NR NR NR BRAWNIN Electron transport chain complex III assembly factor UQCC6 71 Q69YU5 Uqcc6 67 Q8BTC1 CASIMO1 Regulator of cytoskeletal organization, cell migration and proliferation SMIM22 83 K7EJ46 Smim22 86 V9GXA9 CIP2A-BP Negative regulator of PI3K/AKT/NFkB pathway through interaction with CIP2A LINC00665 52...”
- A Unified Transcriptional, Pharmacogenomic, and Gene Dependency Approach to Decipher the Biology, Diagnostic Markers, and Therapeutic Targets Associated with Prostate Cancer Metastasis.
Bacolod, Cancers 2021 - “...ADP-ribosyltransferase 4 (Dombrock blood group) DOK1 Secreted 0.59 C12orf73 chromosome 12 open reading frame 73 Q69YU5 Secreted (curated) Secreted 0.58 MAP3K9 mitogen-activated protein kinase kinase kinase 9 P80192 Secreted (likely) 0.58 SAMD1 sterile alpha motif domain containing 1 Q6SPF0 Cytoplasm, Secreted (curated) 0.58 PCYOX1L prenylcysteine oxidase...”
PF3D7_0709800 conserved protein, unknown function from Plasmodium falciparum 3D7
Aligns to 1:46 / 64 (71.9%), covers 97.8% of PF14990, 76.4 bits
AT4G26055 hypothetical protein from Arabidopsis thaliana
Aligns to 9:52 / 62 (71.0%), covers 100.0% of PF14990, 72.9 bits
- Two neuronal peptides encoded from a single transcript regulate mitochondrial complex III in Drosophila
Bosch, eLife 2022 - “...XP_032827557.1, Lamprey C12orf73 homolog XP_032827559.1, D.melanogaster CG32736 NP_727152.1, D.melanogaster CG42308 NP_001138171.1, Arabidopsis AT5G57080 NP_200518.1, Arabidopsis AT4G26055 NP_001119059.1, Plasmodium PF3D7_0709800 XP_002808771.1, Choanoflagellate ( Salpingoeca urceolata ) m.92763 ( R Richter et al., 2018 ), Choanoflagellate ( Salpingoeca urceolata ) sloth2 homolog is unannotated but present in comp15074_c0_seq2...”
AT5G57080 hypothetical protein from Arabidopsis thaliana
Aligns to 9:52 / 62 (71.0%), covers 97.8% of PF14990, 66.8 bits
- Two neuronal peptides encoded from a single transcript regulate mitochondrial complex III in Drosophila
Bosch, eLife 2022 - “...NP_001129045.1, Lamprey SMIM4 XP_032827557.1, Lamprey C12orf73 homolog XP_032827559.1, D.melanogaster CG32736 NP_727152.1, D.melanogaster CG42308 NP_001138171.1, Arabidopsis AT5G57080 NP_200518.1, Arabidopsis AT4G26055 NP_001119059.1, Plasmodium PF3D7_0709800 XP_002808771.1, Choanoflagellate ( Salpingoeca urceolata ) m.92763 ( R Richter et al., 2018 ), Choanoflagellate ( Salpingoeca urceolata ) sloth2 homolog is unannotated but...”
- Comparison of Leaf Sheath Transcriptome Profiles with Physiological Traits of Bread Wheat Cultivars under Salinity Stress
Takahashi, PloS one 2015 - “...5.03 Os09g0570200 Zinc finger, C2H2-type domain containing protein A_99_P004166 HP629655 5.01 Unknown A_99_P123925 CV764156 4.99 At5g57080 Unknown protein A_99_P184762 CJ726963 4.97 Os10g0571200 Similar to Pyruvate kinase isozyme G A_99_P100350 DR741521 4.96 Unknown A_99_P352331 AK335348 4.88 Os11g0689100 Disease resistance protein family protein A_99_P464282 DR741256 4.85 Os01g0963600 ABA/WDS...”
Or search for genetic data about PF14990 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory