Family Search for PF16126 (DUF4838)
April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.
PF16126 hits 7 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
BT1035 conserved hypothetical protein from Bacteroides thetaiotaomicron VPI-5482
Aligns to 129:452 / 734 (44.1%), covers 99.4% of PF16126, 381.7 bits
- Characterization of a new family of 6-sulfo-N-acetylglucosaminidases
Bains, The Journal of biological chemistry 2023 - “..., 30 ) we were intrigued by the identification of an endo-hexosaminidase from Bacteroides thetaiotaomicron (BT1035) that cleaves the GlcNAc-1,2-Man linkage within complex type N-glycans ( Fig.1 ) to release LacNAc (Gal-1,4-GlcNAc) and SialylLacNAc (Neu5Ac-2,3-Gal-1,4-GlcNAc or Neu5Ac-2,6-Gal-1,4-GlcNAc) fragments. This founding member of the GH163 family contains...”
- “...of Sp_0475 from Streptococcus pneumoniae TIGR4 which contains a DUF4838 domain (PF16126). Although Sp_0475 and BT1035 both contain the DUF4838 domain, the sequence similarity between them is sufficiently low that Sp_0475 has been assigned as the founding member of a new glycoside hydrolase family GH185. Through...”
- Discovery of β-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans
Nihira, The Journal of biological chemistry 2013 - “...(GH92 -mannosidase (BT1032), GH20 -N-acetylhexosaminidase (BT1035), exo--sialidase (BT1036), and GH18 JOURNAL OF BIOLOGICAL CHEMISTRY 27369...”
- “...degraded by -sialidases (BT1036), -galactosidase, -N-acetylhexosaminidase (BT1035), and -mannosidase (BT1032). The resultant ManGlcNAc is transported into the...”
EFB02686.1 β-galactosidase (VadG925;Vvad_PD3900) (EC 3.2.1.23) (see protein)
Aligns to 798:1137 / 1425 (23.9%), covers 100.0% of PF16126, 289.4 bits
MED152_03915 DUF4838 domain-containing protein from Polaribacter sp. MED152
Aligns to 106:419 / 701 (44.8%), covers 97.5% of PF16126, 157.2 bits
SSU0200 hypothetical protein from Streptococcus suis P1/7
Aligns to 231:506 / 628 (43.9%), covers 71.4% of PF16126, 46.1 bits
SPD_0425 hypothetical protein from Streptococcus pneumoniae D39
Aligns to 231:506 / 628 (43.9%), covers 74.5% of PF16126, 45.0 bits
- TCS01 Two-Component System Influenced the Virulence of Streptococcus pneumoniae by Regulating PcpA
Yu, Infection and immunity 2023 (secret) - High-throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae
Liu, Molecular systems biology 2017 - “...repressed (~84fold) (Fig 1 D). Our RNASeq data showed that the genes ( spd_0424 , spd_0425 , lacE1 , lacG1 , lacF1 ) that are downstream of luc, which was driven by a strong constitutive promoter without terminator, were significantly repressed as well ( AppendixFig S1A...”
- The Small Molecule DAM Inhibitor, Pyrimidinedione, Disrupts Streptococcus pneumoniae Biofilm Growth In Vitro
Yadav, PloS one 2015 - “...protein -1.4 (0.002) SPD_0339 conserved hypothetical protein -1.5 (0.03) SPD_0410 conserved hypothetical protein -1.4 (0.05) SPD_0425 conserved hypothetical protein -1.9 (0.02) SPD_0478 conserved hypothetical protein -1.8 (0.05) SPD_0488 conserved hypothetical protein -2.2 (0.001) SPD_0489 conserved hypothetical protein -1.6 (0.007) SPD_0499 conserved hypothetical protein -1.4 (0.002) SPD_0594...”
spr0422 Hypothetical protein from Streptococcus pneumoniae R6
Aligns to 249:524 / 646 (42.7%), covers 74.5% of PF16126, 44.9 bits
SP_0475 hypothetical protein from Streptococcus pneumoniae TIGR4
Aligns to 249:524 / 646 (42.7%), covers 74.5% of PF16126, 44.6 bits
- Characterization of a new family of 6-sulfo-N-acetylglucosaminidases
Bains, The Journal of biological chemistry 2023 - “...ere, we identify and characterize a new, highly specific non-GH20 6-sulfoGlcNAcase from Streptococcus pneumoniae TIGR4, Sp_0475 with a greater than 110,000-fold preference toward N-acetyl-- D -glucosamine-6-sulfate substrates over the nonsulfated version. Sp_0475 shares distant sequence homology with enzymes of GH20 and with the newly formed GH163...”
- “...bioinformatics we provide insight into the substrate specificity, mechanism, and key active site residues of Sp_0475. Enzymes of the GH185 family follow a substrate-assisted mechanism, consistent with their distant homology to the GH20 family, but the catalytic residues involved are quite different. Taken together, our results...”
- Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms
van, Nature methods 2009 - “...a small sub-network including four PTS genes, the hydrolase bgaA (SP_0648) and an uncharacterized gene SP_0475 ( Fig. 5c ). Discussion Tn-seq can be used to determine the fitness conferred by single genes and to map genetic interactions in microorganisms. Unlike existing methods, Tn-seq does not...”
- “...containing interactions between ccpA , four PTS genes, the -galactosidase bgaA and the uncharacterized gene SP_0475. In other bacterial species, cytoplasmic -galactosidases play an important role in lactose metabolism. However, in S. pneumoniae bgaA has a cell surface localization, and has so far only been associated...”
Or search for genetic data about PF16126 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory