Family Search for PF16153 (DUF4861)

April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.

PF16153 hits 3 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

BT3233 predicted glycosaminoglycan binding protein (DUF4861) from Bacteroides thetaiotaomicron VPI-5482
BT3233 hypothetical protein from Bacteroides thetaiotaomicron VPI-5482
Aligns to 29:412 / 414 (92.8%), covers 100.0% of PF16153, 364.5 bits

• mutant phenotype: Specifically important in carbon source Heparin sodium salt from porcine intestinal mucosa; carbon source Chondroitin sulfate A sodium salt from bovine trachea; and perhaps carbon source hyaluronic acid. Belongs to the galactose mutarotase-like domain superfamily, which are thought to be carbohydrate-binding proteins (see InterPro IPR011013). Also, this family (PF16153) is predicted by the PFam curators to bind carbohydrates because of its conserved proximity to glycosyl hydrolyase family 88.
• Functional genetics of human gut commensal Bacteroides thetaiotaomicron reveals metabolic requirements for growth across environments
  Liu, Cell reports 2021
  • “...), suggesting that BT4410 and BT4411 function together in HA catabolism. Finally, we found that BT3233 was important for growth on the following 3 GAGs: CS (mean fitness, 1.6), HA (mean fitness, 0.7), and heparin (mean fitness, 2.2) ( Figure 3G ). BT3233 is a member...”
  • “...binding domains (galactose mutarotase-like domain, InterPro IPR011013) ( Mitchell et al., 2019 ). Homologs of BT3233 are mostly confined to Bacteroides species, including a number of human commensals. Using an archived transposon mutant, we confirmed that BT3233 is important for growth on CS ( Figure 3H...”

PLH7_FORAG / T2KN67 Uncharacterized protein P7; Polysaccharide utilization locus H protein P7; PUL H protein P7 from Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) (see paper)
Aligns to 31:392 / 395 (91.6%), covers 100.0% of PF16153, 291.1 bits

• function: May be involved in ulvan degradation (Probable). Ulvan is the main polysaccharide component of the Ulvales (green seaweed) cell wall. It is composed of disaccharide building blocks comprising 3-sulfated rhamnose (Rha3S) linked to D-glucuronic acid (GlcA), L-iduronic acid (IduA), or D-xylose (Xyl) (Probable).

B2UQG1 Glycosyl hydrolase family 88 from Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc)
Aligns to 385:741 / 744 (48.0%), covers 100.0% of PF16153, 208.8 bits

• The evolution and competitive strategies of Akkermansia muciniphila in gut
  Kim, Gut microbes 2022
  • “...1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 B2UQG1 GH 109 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 B2UL75...”

Or search for genetic data about PF16153 in the Fitness Browser