Family Search for PF16315 (DUF4955)

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PF16315 hits 1 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

BT3328 hyaluronic acid endo-lyase (PL29) from Bacteroides thetaiotaomicron VPI-5482
BT3328, BT_3328 hypothetical protein from Bacteroides thetaiotaomicron VPI-5482
WP_008767539 DUF4955 domain-containing protein from Bacteroides thetaiotaomicron
Aligns to 716:867 / 868 (17.5%), covers 100.0% of PF16315, 246.2 bits

• mutant phenotype: Specifically important in carbon source Hyaluronic acid sodium salt from Streptococcus equi. In vitro, it also degrades other glycosaminoglycans such as chondroitin sulfate and dermatan sulfate (PMCID:PMC6240882), but it is not important for utilizing chondroitin sulfate. We do not have fitness data for dermatan sulfate.
• Human Gut Microbiota and Drug Metabolism
  Pant, Microbial ecology 2023
  • “...the genome of gut bacteria Bacteroides cellulosilyticus [ 137 ]. Another study reported a gene (BT_3328) encoding a novel polysaccharide lyase, BtCDH from B. thetaiotaomicron , capable of degrading the dermatan sulfate, chondroitin sulfate, and hyaluronic acid of glycosaminoglycans, an important player in hostmicrobial interactions [...”
• Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species
  Overbeeke, Frontiers in microbiology 2022
  • “...act on both compounds. We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown. Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders,...”
  • “...et al., 2014 ). Additionally, a novel surface lyase with a high affinity for CS (BT_3328) has recently been identified ( Ndeh et al., 2018 , 2020 ). Although B . theta PUL architecture for CS and HA degradation has been well-studied, the ability of other...”
• Utilization of glycosaminoglycans by the human gut microbiota: participating bacteria and their enzymatic machineries
  Rawat, Gut microbes 2022
  • “...proven to be SGBPs. 69 Within the PUL, four PLs are encoded, viz . BT3324, BT3328, BT3350, and the distal BT4410. Among them, BT3324 and BT3350 belong to polysaccharide lyase family 8 (PL8), whereas BT3328 and BT4410 belong to PL29 and PL33 families, respectively. 80 ,...”
  • “...periplasm. However, Ndeh et al., 2018 101 showed the presence of a surface-localized polysaccharide lyase (BT3328), which was identified as the founding member of the PL29 family. BT3328-PL29 was shown to cleave CS, DS, and HA through endolytic -elimination. Product analysis revealed that a series of...”
• Host glycan utilization within the Bacteroidetes Sus-like paradigm
  Brown, Glycobiology 2021
  • “...pair and a single extracellular enzyme, a PL29-family BT3328, the first identified in this family (Ndeh et al. 2018). CS/DS/HA recognition requires two SGBPs,...”
• Functional genetics of human gut commensal Bacteroides thetaiotaomicron reveals metabolic requirements for growth across environments
  Liu, Cell reports 2021
  • “...given that only CS contains sulfated sugars. The polysaccharide lyase family PL29 enzyme encoded by BT3328 was only important for fitness on HA, despite the activity of this enzyme against both HA and CS in vitro ( Ndeh et al., 2018 ). The first GAG utilization...”
• Metabolism of multiple glycosaminoglycans by Bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus
  Ndeh, Nature communications 2020
  • “...PL33 are upregulated 4 , 13 . PUL CS/DS/HA encodes three PLs (BT3324 PL8 , BT3328 PL29 and BT3350 PL8 ), one GH88 (BT3348 GH88 ), 2-O-sulfatases (BT3333-S1_15 6S-sulf and BT3349-S1_27 4S-sulf) , a single SusC/D-like transporter system (BT3331 susD -BT3332 susC ), a hybrid two-component...”
  • “...measurable affinity, similar to that of BT3329 SGBP (Supplementary Table 1 ). The surface lyase, BT3328 PL29 , showed its highest enzymatic activity and affinity against CS-A>CS-C>HA and little activity on DS, differing from previous data 18 . End point assays, the total number of glycosidic...”
• The human gut microbe Bacteroides thetaiotaomicron encodes the founding member of a novel glycosaminoglycan-degrading polysaccharide lyase family PL29
  Ndeh, The Journal of biological chemistry 2018
  • “...Here, we investigated a gene of unknown function (BT_3328) from the chondroitin sulfate (CS) utilization locus of B. thetaiotaomicron. NMR and UV spectroscopic...”
  • “...CS PUL of B. thetaiotaomicron, three of such genes BT_3328, BT_3329 and BT_3330 (all of unknown function) appear in a distinct operon (BT_332830) conserved in...”
• Species-specific dynamic responses of gut bacteria to a mammalian glycan
  Raghavan, Journal of bacteriology 2015
  • “...the B. thetaiotaomicron CS PUL includes three genes (BT3328, BT3329, and BT3330) encoding proteins of unknown function predicted to localize to the outer...”
• The human gut microbe Bacteroides thetaiotaomicron encodes the founding member of a novel glycosaminoglycan-degrading polysaccharide lyase family PL29.
  Ndeh, The Journal of biological chemistry 2018
  • GeneRIF: BtCDH is a cell surfacae glucosaminoglycan-degrading polysaccharide lyase from the PL29 family.

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