Family Search for PF20173 (ZnF_RZ-type)
PF20173 hits 19 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
AFUA_2G05180 NF-X1 finger and helicase domain protein, putative from Aspergillus fumigatus Af293
Aligns to 1848:1901 / 1914 (2.8%), covers 100.0% of PF20173, 81.0 bits
ZNFX1_MOUSE / Q8R151 NFX1-type zinc finger-containing protein 1 from Mus musculus (Mouse) (see paper)
NP_001411605 NFX1-type zinc finger-containing protein 1 isoform a from Mus musculus
Aligns to 1826:1880 / 1909 (2.9%), covers 85.2% of PF20173, 79.2 bits
- function: RNA-binding protein that initiates the antiviral response and is required to restrict the replication of RNA viruses (PubMed:31685995). Acts as a double-stranded RNA (dsRNA) sensor that recognizes viral RNA and then interacts with MAVS to initiate the type I interferon response (PubMed:31685995). Also required for immunity against some bacteria, such as mycobacteria (By similarity).
subunit: Interacts with MAVS.
disruption phenotype: Mice are viable without obvious physiological or behavioral abnormalities (PubMed:31685995). They however display impaired innate immune responses against RNA virus infection by producing less type I interferons (IFNs) (PubMed:31685995). - Molecular characterization of accripin11, a soluble shell protein with an acidic C-terminus, identified in the prismatic layer of the Mediterranean fan mussel Pinna nobilis (Bivalvia, Pteriomorphia)
Khurshid, FEBS open bio 2023 - “...the cysteine pattern (shaded blocks) with that of one zinc finger protein (SwissProt accession number Q8R151) and one keratinassociated protein (SwissProt accession number Q64507) of Mus musculus . Fig.S3. 3D structure prediction with AlphaFold2 of the 6 putative molluscan shell sequences that were found to be...”
- Norbergenin prevents LPS-induced inflammatory responses in macrophages through inhibiting NFκB, MAPK and STAT3 activation and blocking metabolic reprogramming.
Li, Frontiers in immunology 2023 - “...metabolic pathway. Category Gene name UniProt ID Protein name Log2 FC Regulation Inflammatory response Znfx1 Q8R151 NFX1-type zinc finger-containing protein 1 -0.988 Down Epsti1 Q8VDI1 Epithelial-stromal interaction protein 1 -1.246 Down Lcp2 Q60787 Lymphocyte cytosolic protein 2 -1.192 Down Tnfaip2 Q61333 Tumor necrosis factor alpha-induced protein...”
- Genomic variants in an inbred mouse model predict mania-like behaviors
Saul, PloS one 2018 - “...Npas2 P97460 Missense L481P 0.23 chr2:25363944 C/T Uap1l1 Q3TW96 Missense G300D 0.13 chr2:167036853 C/T Znfx1 Q8R151 Missense G1868S 0.00 chr3:19980665 T/G Cp G3X8Q5 Missense C712G 0.01 chr3:19987423 G/A Cp G3X9T8 Missense S903N 0.22 chr3:20005467 T/C Cp G3UXD2 Missense, Splice V23A --- chr3:20012808 C/A Hps3 E9PZY1 Missense...”
- ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing Prkaa2 mRNA.
Liu, JCI insight 2024 - GeneRIF: ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing Prkaa2 mRNA.
- ZNFX1 antisense RNA1 promotes antiviral innate immune responses via modulating ZNFX1 function.
Jia, Journal of medical virology 2023 (PubMed)- GeneRIF: ZNFX1 antisense RNA1 promotes antiviral innate immune responses via modulating ZNFX1 function.
- Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS.
Wang, Nature cell biology 2019 (PubMed)- GeneRIF: Mitochondria-localized ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS.
R213A_DANRE / A0A0R4IBK5 E3 ubiquitin-protein ligase rnf213-alpha; E3 ubiquitin-lipopolysaccharide ligase rnf213-alpha; Mysterin-A; Mysterin-alpha; RING finger protein 213-A; RING finger protein 213-alpha; EC 2.3.2.27; EC 3.6.4.-; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see 4 papers)
XP_021330430 E3 ubiquitin-protein ligase rnf213-alpha from Danio rerio
Aligns to 4494:4547 / 5209 (1.0%), covers 96.3% of PF20173, 79.1 bits
- function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (By similarity). Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (By similarity). Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (By similarity). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (PubMed:30705059). Also regulates lipotoxicity by inhibiting desaturation of fatty acids (By similarity). Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger (By similarity). Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of proteins downstream of rspo3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (By similarity). Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (By similarity). Also involved in neuromuscular regulation (PubMed:26530008).
catalytic activity: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
catalytic activity: ATP + H2O = ADP + H(+) + phosphate (RHEA:13065)
disruption phenotype: Morpholino knockdown leads to a slight reduction in body size, a small eye and a wavy trunk (PubMed:21799892). Fishes develope abnormal angiogenesis in intersegmental vessels and cranial secondary vessels (PubMed:21799892, PubMed:27125596). Abnormal sprouting vessels are observed in the trunk and head regions, while axial trunk vessels, the dorsal aorta and posterior cardinal vein proceed almost normally (PubMed:21799892). Morpholino knockdown in larvae leads to a reduction in fast myofibrils and immature projection of primary motoneurons, leading to severe motor deficits (PubMed:26530008). Decreased lipid droplet formation (PubMed:30705059). - Mutations of RNF213 are responsible for sporadic cerebral cavernous malformation and lead to a mulberry-like cluster in zebrafish.
Lin, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2021 - GeneRIF: Mutations of RNF213 are responsible for sporadic cerebral cavernous malformation and lead to a mulberry-like cluster in zebrafish.
- Mutation of rnf213a by TALEN causes abnormal angiogenesis and circulation defects in zebrafish.
Wen, Brain research 2016 (PubMed)- GeneRIF: Rnf213a mutant zebrafish developed abnormal angiogenesis in intersegmental vessels and cranial secondary vessels. Endothelial cells exhibited the defects in morphogenesis and formation of vascular tubes despite normal cell to cell contacts.
NP_001376200 NFX1-type zinc finger-containing protein 1 from Rattus norvegicus
Aligns to 1824:1879 / 1907 (2.9%), covers 85.2% of PF20173, 79.1 bits
LOC101857249 NFX1-type zinc finger-containing protein 1 from Aplysia californica
Aligns to 1797:1851 / 1887 (2.9%), covers 87.0% of PF20173, 77.7 bits
- In search of the Aplysia immunome: an in silico study
Kron, BMC genomics 2022 - “...conserved domains, PANTHER annotation from IPS (PTHR10887:SF470), and homology search, three potential ZNFX1 genes (LOC101862822, LOC101857249, LOC101851452) were identified. Several DNA-damage detecting proteins have also been implicated in cytosolic sensing of viral nucleotide [ 32 , 33 ]. Putative orthologs of several of these, including all...”
- “...IPR013785 4 LOC101856483, LOC101856246, LOC101847430, LOC101847031 ZNFX1 IPR041677, IPR041679, IPR000967, IPR016024 IPR045055, IPR041677, IPR041679 3 LOC101857249, LOC101862822, LOC101851452 RNAi RNA interference (RNAi) is another potent antiviral mechanism of particular importance in arthropods [ 88 ]. Key components of the RISC RNAi complex, including Dicer (LOC101848232), two...”
U3IUP4 Zinc finger NFX1-type containing 1 from Anas platyrhynchos platyrhynchos
Aligns to 2026:2081 / 2110 (2.7%), covers 85.2% of PF20173, 77.0 bits
ZNFX1_HUMAN / Q9P2E3 NFX1-type zinc finger-containing protein 1 from Homo sapiens (Human) (see 3 papers)
Aligns to 1834:1889 / 1918 (2.9%), covers 85.2% of PF20173, 76.2 bits
U6CWZ8 NFX1-type zinc finger-containing protein 1 from Neovison vison
Aligns to 1837:1892 / 1921 (2.9%), covers 87.0% of PF20173, 75.9 bits
I3L5Z9 Zinc finger NFX1-type containing 1 from Sus scrofa
Aligns to 1842:1897 / 1926 (2.9%), covers 87.0% of PF20173, 75.6 bits
- Proteomic Characterization of PAMs with PRRSV-ADE Infection.
Xu, Viruses 2022 - “...0.018624 F1SCY2 Interferon-induced protein with tetratricopeptide repeats 3 (IFIT3)/Interferon-stimulated gene 60 (ISG60) IFIT3/ISG60 0.426091944 0.000606 I3L5Z9 Zinc finger NFX1-type containing 1 (ZNFX1) ZNFX1 0.408049746 0.000009 I3LB04 Interferon-stimulated gene 20 (ISG20) ISG20 0.519256582 0.004996 J7FIC7 Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1)/Interferon-stimulated gene 56 (ISG56) IFIT1/ISG56 0.462410477...”
A0A662Z298 E3 ubiquitin-protein ligase RNF213 from Acipenser ruthenus
Aligns to 4423:4478 / 5134 (1.1%), covers 96.3% of PF20173, 70.2 bits
NP_001243000 E3 ubiquitin-protein ligase RNF213 isoform 3 from Homo sapiens
Aligns to 4490:4545 / 5207 (1.1%), covers 85.2% of PF20173, 70.1 bits
RN213_HUMAN / Q63HN8 E3 ubiquitin-protein ligase RNF213; ALK lymphoma oligomerization partner on chromosome 17; E3 ubiquitin-lipopolysaccharide ligase RNF213; Mysterin; RING finger protein 213; EC 2.3.2.27; EC 3.6.4.-; EC 2.3.2.- from Homo sapiens (Human) (see 28 papers)
Aligns to 4490:4545 / 5207 (1.1%), covers 85.2% of PF20173, 70.1 bits
- function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PubMed:21799892, PubMed:26126547, PubMed:26278786, PubMed:26766444, PubMed:30705059, PubMed:32139119, PubMed:34012115). Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc- finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (PubMed:34012115). Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains (PubMed:34012115). Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (PubMed:34012115). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (PubMed:30705059). Also regulates lipotoxicity by inhibiting desaturation of fatty acids (PubMed:30846318). Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger: mediates 'Lys-63'-linked ubiquitination of target proteins (PubMed:32139119, PubMed:33842849). Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (PubMed:26766444). Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (PubMed:34012115).
catalytic activity: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
catalytic activity: ATP + H2O = ADP + H(+) + phosphate (RHEA:13065)
subunit: Monomer (By similarity). Interacts with UBE2L3/UBCH7; UBE2L3/UBCH7 is the most efficient ubiquitin-conjugating enzyme E2 for the ubiquitin ligase activity (By similarity). Interacts with UBE2N/UBC13; promoting 'Lys-63'-linked ubiquitination of target proteins (PubMed:32139119, PubMed:33842849).
subunit: (Microbial infection) Interacts with M.tuberculosis protein Rv3655c, which impairs caspase-8 activation and suppresses macrophage apoptosis by blocking the extrinsic pathway. - Ligandability of E3 Ligases for Targeted Protein Degradation Applications.
Belcher, Biochemistry 2023 - “...C320 *** RNF149 Q8NC42 C295 *** RNF167 Q9H6Y7 C271 *** RNF180 Q86T96 C28 *** RNF213 Q63HN8 C614 *** UBR1 Q8IWV7 C180, C1603 *** UBR2 Q8IWV8 C1360, C1619 *** , C576 UBR4 Q5T4S7 C1358, C2554 *** , C4049, C2222, C1274 ZNF598 Q86UK7 C456 *** KCTD21 Q4G0X4 C166...”
- RNF213 loss-of-function promotes pathological angiogenesis in moyamoya disease via the Hippo pathway.
Ye, Brain : a journal of neurology 2023 - “...the three-frame translation. ( B ) The protein structure of the wild-type (WT) RNF213 protein (Q63HN8) was modelled with 100.0% confidence based on the single highest-scoring template of the well-known mouse Rnf213 (c7oikA). The benign CNV deletion of RNF213 showed a high identity (99.42%) with WT,...”
- Count Dracula Resurrected: Proteomic Analysis of Vlad III the Impaler's Documents by EVA Technology and Mass Spectrometry.
Pittalà, Analytical chemistry 2023 - “...Q9H2U2 inorganic pyrophosphatase 2, mitochondrial EENGIPMKK M ox pGlu low tissue specificity YES x RNF213 Q63HN8 E3 ubiquitin-protein ligase RNF213 WQKSIVEELCAWVEKFINVK W ox-ky W oxolactone low tissue specificity YES x ROBO4 Q8WZ75 roundabout homolog4 MGSGGDSLLGGR acetyl (protein N-term)M ox adipose tissue YES x ROPN1L Q96C74 ropporin-1-like...”
- The Protective Action of Metformin against Pro-Inflammatory Cytokine-Induced Human Islet Cell Damage and the Mechanisms Involved.
Giusti, Cells 2022 - “...Q8N339 METALLOTHIONEIN-1M (PTHR23299:SF50) MT1M 3.11 2.49 Q09666 NEUROBLAST DIFFERENTIATION-ASSOCIATED PROTEIN AHNAK (PTHR23348:SF41) AHNAK 9.48 0.31 Q63HN8 E3 UBIQUITIN-PROTEIN LIGASE RNF213 (PTHR22605:SF16) RNF213 10.35 8.25 O95786 ATP-DEPENDENT RNA HELICASE DDX58-RELATED (PTHR14074:SF16) DDX58 11.21 10.63 P69905 HEMOGLOBIN SUBUNIT ALPHA (PTHR11442:SF48) HBA1 23.19 34.96 Q13113 PDZK1-INTERACTING PROTEIN 1 (PTHR15296:SF0)...”
- Identification of HO-1 as a novel biomarker for graft acute cellular rejection and prognosis prediction after liver transplantation
Jia, Annals of translational medicine 2020 - “...AK1 1.448 Q9GZP4 PITH domain containing 1 PITHD1 1.444 P27348 14-3-3 protein theta YWHAQ 1.436 Q63HN8 Ring finger protein 213 RNF213 1.435 P53004 Biliverdin reductase A BLVRA 1.432 P52907 F-actin-capping protein subunit alpha-1 CAPZA1 1.431 Q99873 Protein arginine methyltransferase 1 PRMT1 1.431 O00151 PDZ and LIM...”
- Proteomic profile of follicular fluid from patients with polycystic ovary syndrome (PCOS) submitted to in vitro fertilization (IVF) compared to oocyte donors
Domingues, JBRA assisted reproduction 2019 - “...NA NA 1 Q53GS7 Nucleoporin GLE1 nucleic acid binding(PC00171);transfer/carrier protein(PC00219) nucleic acid binding(GO:0005488) NA 1 Q63HN8 E3 ubiquitin-protein ligase RNF213 NA NA NA 1 Q6YP21 Kynurenine--oxoglutarate transaminase 3 transaminase(PC00220) transaminase activity(GO:0003824) NA 1 Q70CQ2 Ubiquitin carboxyl-terminal hydrolase 34 NA NA NA 1 Q7Z6M1 Rab9 effector protein...”
- SAMHD1 Enhances Chikungunya and Zika Virus Replication in Human Skin Fibroblasts.
Wichit, International journal of molecular sciences 2019 - “...LAP3 P28838 1.049 Interferon-induced 35 kDa protein IFI35 P80217 1.015 E3 ubiquitin-protein ligase RNF213 RNF213 Q63HN8 0.895 Gamma-interferon-inducible protein 16 IFI16 Q16666 0.878 Double-stranded RNA-activated protein kinase EIF2AK2 * P19525 0.838 HLA class I histocompatibility antigen, A-69 alpha chain HLA-A P10316 0.752 Zika virus infected cells...”
- Novel and recurrent RNF213 variants in Japanese pediatric patients with moyamoya disease.
Akagawa, Human genome variation 2018 - “...lobe ( i ). Figure 2 ( a ) Protein structure of RNF213, based on Q63HN8 (RN213_HUMAN) in the InterPro ( http://www.ebi.ac.uk/interpro/ ) database. ( b ) DNA sequence chromatograms of the three heterozygous missense variants detected in the present study. ( c ) Multiple alignment...”
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XP_038943469 E3 ubiquitin-protein ligase RNF213 isoform X1 from Rattus norvegicus
Aligns to 4490:4544 / 5201 (1.1%), covers 81.5% of PF20173, 67.8 bits
F1M0R1 Ring finger protein 213 from Rattus norvegicus
Aligns to 4415:4469 / 5126 (1.1%), covers 81.5% of PF20173, 67.8 bits
DDB_G0278761 NF-X1-type zinc finger-containing protein from Dictyostelium discoideum AX4
Aligns to 1717:1772 / 1793 (3.1%), covers 96.3% of PF20173, 67.2 bits
XP_006534693 E3 ubiquitin-protein ligase RNF213 isoform X1 from Mus musculus
Aligns to 4480:4535 / 5192 (1.1%), covers 85.2% of PF20173, 66.8 bits
- RNF213 loss-of-function promotes pathological angiogenesis in moyamoya disease via the Hippo pathway.
Ye, Brain : a journal of neurology 2023 - GeneRIF: RNF213 loss-of-function promotes pathological angiogenesis in moyamoya disease via the Hippo pathway.
- Rnf-213 Knockout Induces Pericyte Reduction and Blood-Brain Barrier Impairment in Mouse.
Li, Molecular neurobiology 2023 (PubMed)- GeneRIF: Rnf-213 Knockout Induces Pericyte Reduction and Blood-Brain Barrier Impairment in Mouse.
- Knockout of Rnf213 Ameliorates Cerebral Ischemic-reperfusion Injury by Inhibiting Neuronal Apoptosis Through the Akt/GSK-3β/β-catenin/Bcl-2 Pathway.
Li, Neuroscience 2023 (PubMed)- GeneRIF: Knockout of Rnf213 Ameliorates Cerebral Ischemic-reperfusion Injury by Inhibiting Neuronal Apoptosis Through the Akt/GSK-3beta/beta-catenin/Bcl-2 Pathway.
- Suppression of RNF213, a susceptibility gene for moyamoya disease, inhibits endoplasmic reticulum stress through SEL1L upregulation.
Ahmed, Biochemical and biophysical research communications 2022 (PubMed)- GeneRIF: Suppression of RNF213, a susceptibility gene for moyamoya disease, inhibits endoplasmic reticulum stress through SEL1L upregulation.
- Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.
Ahel, eLife 2020 - GeneRIF: Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.
- S-nitrosylation of E3 ubiquitin-protein ligase RNF213 alters non-canonical Wnt/Ca+2 signaling in the P301S mouse model of tauopathy.
Amal, Translational psychiatry 2019 - GeneRIF: Study shows that S-nitrosylation of E3 ubiquitin-protein ligase RNF213 alters non-canonical Wnt/Ca+2 signaling in the P301S mouse model of tauopathy.
- Dysregulation of RNF213 promotes cerebral hypoperfusion.
Morimoto, Scientific reports 2018 - GeneRIF: results are indicative of the essential role of RNF213 in the maintenance of cerebral blood flow .
- Transient middle cerebral artery occlusion in mice induces neuronal expression of RNF213, a susceptibility gene for moyamoya disease.
Sato-Maeda, Brain research 2016 (PubMed)- GeneRIF: The Rnf213 gene was up-regulated in the ischemic brain, especially at the penumbra area, as early as 6 h after transient middle cerebral artery occlusion
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RN213_MOUSE / E9Q555 E3 ubiquitin-protein ligase RNF213; E3 ubiquitin-lipopolysaccharide ligase RNF213; Mysterin; RING finger protein 213; EC 2.3.2.27; EC 3.6.4.-; EC 2.3.2.- from Mus musculus (Mouse) (see 6 papers)
Aligns to 4436:4491 / 5148 (1.1%), covers 85.2% of PF20173, 66.8 bits
- function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PubMed:32573437). Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (PubMed:34012115). Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains (By similarity). Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (By similarity). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (By similarity). Also regulates lipotoxicity by inhibiting desaturation of fatty acids (By similarity). Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger: mediates 'Lys- 63'-linked ubiquitination of target proteins (By similarity). Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (By similarity). Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (PubMed:32573437).
catalytic activity: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
catalytic activity: ATP + H2O = ADP + H(+) + phosphate (RHEA:13065)
subunit: Monomer (PubMed:32573437). Interacts with UBE2L3/UBCH7; UBE2L3/UBCH7 is the most efficient ubiquitin-conjugating enzyme E2 for the ubiquitin ligase activity (PubMed:32573437). Interacts with UBE2N/UBC13; promoting 'Lys-63'-linked ubiquitination of target proteins (By similarity).
disruption phenotype: No visible phenotype (PubMed:23410753, PubMed:24440776). Males and female are fertile and produce normal-sized litters (PubMed:23410753, PubMed:24440776). Mice do not spontaneously develop Moyamoya disease, a chronic occlusive cerebrovascular disease (PubMed:24440776). Mice are however more susceptible to Rift Valley fever virus infection (PubMed:33420513). Mice show elevated expression of MMP9 (PubMed:25383461). - The Proteomic Signature of Intestinal Acute Rejection in the Mouse.
Oltean, Metabolites 2021 - “...2.38 Antimicrobial Inflammatory response Q99KQ4 Nampt Nicotinamide phosphoribosyltransferase 2.12 Cytokine, Glycosyltransferase Cell death and survival E9Q555 Rnf213 E3 ubiquitin-protein ligase RNF213 1.82 Hydrolase, Transferase Angiogenesis P01899 H2-d1 H-2 class I histocompatibility antigen, D-B alpha chain 1.69 Binding protein Immunology Q9R233 Tapbp Tapasin 1.59 Binding protein Immunology...”
- Quantitative Proteomics Reveals Docosahexaenoic Acid-Mediated Neuroprotective Effects in Lipopolysaccharide-Stimulated Microglial Cells
Yang, Journal of proteome research 2020 - “...cell nuclear differentiation antigen-like protein MNDAL 2.67 n.d. 3.25 Q64337 sequestosome-1 SQSTM1 1.86 0.22 2.02 E9Q555 E3 ubiquitin-protein ligase RNF213 1.66 2.01 2.03 Q8VI94 2-5-oligoadenylate synthase-likeprotein1 OASL1 1.54 1 1.94 Increased in DHA, Lower/Unchanged in LPS Q3UH60 disco-interacting protein 2-B DIP2B n.d. 2.43* n.d. Q91W86 vacuolar...”
- iTRAQ‑based proteomic analysis of endotoxin tolerance induced by lipopolysaccharide
Zhang, Molecular medicine reports 2019 - “...1.363 20 Q9Z0J0 Npc2 Epididymal secretory protein E1 1.362 21 Q9JHK5 Plek Pleckstrin 1.336 22 E9Q555 Rnf213 E3 ubiquitin-protein ligase RNF213 1.328 23 D0QMC3 Mndal Myeloid cell nuclear differentiation antigen-like protein 1.327 24 P20108 Prdx3 Thioredoxin-dependent peroxide reductase, mitochondrial 1.304 25 P97429 Anxa4 Annexin A4 1.298...”
- The ubiquitin ligase Cullin5SOCS2 regulates NDR1/STK38 stability and NF-κB transactivation
Paul, Scientific reports 2017 - “...3.285 1.189 12 169.43 Q8BW84 Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial Pet112 3.543 1.180 5 61.509 E9Q555 E3 ubiquitin-protein ligase RNF213 Rnf213 2.675 1.138 18 584.49 Q8R5C5 Beta-centractin Actr1b 1.997 1.098 6 42.281 Q9DCJ5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Ndufa8 3.935 1.098 5 19.992...”
- Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway.
Zhong, Proteomics 2015 - “...1.7 Ranbp3 Ran-binding protein 3 Q9CT10 S283 2.4 T56 2.4 Rnf213 E3 ubiquitin-protein ligase RNF213 E9Q555 T46 3.3 Rragc Ras-related GTP-binding protein C Q99K70 S94 1.9 T95 2.1 Sept9 Septin-9 Q80UG5 S30 1.8 Snrnp70 U1 small nuclear ribonucleoprotein 70 kDa Q62376 S408 7.6 Trim28 Transcription intermediary...”
- iTRAQ protein profile analysis of neuroblastoma (NA) cells infected with the rabies viruses rHep-Flury and Hep-dG.
Yang, Frontiers in microbiology 2015 - “...3 1.375 0.026 Ddx58 protein Ddx58 A1L0V6 2 1.378 0.034 E3 ubiquitin-protein ligase RNF213 Rnf213 E9Q555 16 1.391 0.044 Signal transducer and activator of transcription 3 Stat3 Q6GU23 2 1.415 0.017 mRNA H2-D1 Q61892 1 1.454 0.046 Discoidin domain-containing receptor 2 Ddr2 Q62371 2 1.490 0.053...”
- Comparative proteomic analysis identifies age-dependent increases in the abundance of specific proteins after deletion of the small heat shock proteins αA- and αB-crystallin.
Andley, Biochemistry 2013 - “...6171 catenin -1, Q02248 8 85 1.12 3.27 3.65 MCG140784, Q792Z1 4 26 Uncharacterized protein, E9Q555 4 584 -1-antitrypsin 14, Q00897 3 46 Nesprin-1, Q6ZWR6 3 1010 Liver carboxylesterase N, P23953 3 61 Alpha-2-macroglobulin, Q61838 2 166 Glutamate dehydrogenase 1, mitochondrial P26443 2 61 6278 Histone...”
E1BHN4 Ring finger protein 213 from Bos taurus
Aligns to 4293:4348 / 5005 (1.1%), covers 85.2% of PF20173, 65.0 bits
R213B_DANRE / A0A0R4I9Y1 E3 ubiquitin-protein ligase rnf213-beta; E3 ubiquitin-lipopolysaccharide ligase rnf213-beta; Mysterin-B; Mysterin-beta; RING finger protein 213-B; RING finger protein 213-beta; EC 2.3.2.27; EC 3.6.4.-; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see 2 papers)
Aligns to 4437:4491 / 5061 (1.1%), covers 85.2% of PF20173, 58.3 bits
- function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity. Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ- type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS. Ubiquitination of LPS triggers cell- autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (By similarity). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (By similarity). Also regulates lipotoxicity by inhibiting desaturation of fatty acids. Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger. Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of proteins downstream of rspo3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression. Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (By similarity).
catalytic activity: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
catalytic activity: ATP + H2O = ADP + H(+) + phosphate (RHEA:13065)
disruption phenotype: No visible phenotype (PubMed:21799892, PubMed:26530008). No vascular phenotype (PubMed:21799892, PubMed:26530008).
Or search for genetic data about PF20173 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory