Family Search for PF20173 (DUF6539)
PF20173 hits 17 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
AFUA_2G05180 NF-X1 finger and helicase domain protein, putative from Aspergillus fumigatus Af293
Aligns to 1848:1901 / 1914 (2.8%), covers 100.0% of PF20173, 79.7 bits
ZNFX1_MOUSE / Q8R151 NFX1-type zinc finger-containing protein 1 from Mus musculus (Mouse) (see paper)
XP_006500519 NFX1-type zinc finger-containing protein 1 isoform X1 from Mus musculus
Aligns to 1825:1880 / 1909 (2.9%), covers 85.2% of PF20173, 78.2 bits
NP_001376200 NFX1-type zinc finger-containing protein 1 from Rattus norvegicus
Aligns to 1824:1879 / 1907 (2.9%), covers 83.3% of PF20173, 78.2 bits
R213A_DANRE / A0A0R4IBK5 E3 ubiquitin-protein ligase rnf213-alpha; E3 ubiquitin-lipopolysaccharide ligase rnf213-alpha; Mysterin-A; Mysterin-alpha; RING finger protein 213-A; RING finger protein 213-alpha; EC 2.3.2.27; EC 3.6.4.-; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see 4 papers)
XP_021330430 E3 ubiquitin-protein ligase rnf213-alpha from Danio rerio
Aligns to 4494:4547 / 5209 (1.0%), covers 96.3% of PF20173, 77.1 bits
- function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (By similarity). Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (By similarity). Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (By similarity). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (PubMed:30705059). Also regulates lipotoxicity by inhibiting desaturation of fatty acids (By similarity). Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger (By similarity). Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of proteins downstream of rspo3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (By similarity). Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (By similarity). Also involved in neuromuscular regulation (PubMed:26530008).
catalytic activity: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
catalytic activity: ATP + H2O = ADP + H(+) + phosphate (RHEA:13065)
disruption phenotype: Morpholino knockdown leads to a slight reduction in body size, a small eye and a wavy trunk (PubMed:21799892). Fishes develope abnormal angiogenesis in intersegmental vessels and cranial secondary vessels (PubMed:21799892, PubMed:27125596). Abnormal sprouting vessels are observed in the trunk and head regions, while axial trunk vessels, the dorsal aorta and posterior cardinal vein proceed almost normally (PubMed:21799892). Morpholino knockdown in larvae leads to a reduction in fast myofibrils and immature projection of primary motoneurons, leading to severe motor deficits (PubMed:26530008). Decreased lipid droplet formation (PubMed:30705059). - Mutations of RNF213 are responsible for sporadic cerebral cavernous malformation and lead to a mulberry-like cluster in zebrafish.
Lin, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2021 - GeneRIF: Mutations of RNF213 are responsible for sporadic cerebral cavernous malformation and lead to a mulberry-like cluster in zebrafish.
- Mutation of rnf213a by TALEN causes abnormal angiogenesis and circulation defects in zebrafish.
Wen, Brain research 2016 (PubMed)- GeneRIF: Rnf213a mutant zebrafish developed abnormal angiogenesis in intersegmental vessels and cranial secondary vessels. Endothelial cells exhibited the defects in morphogenesis and formation of vascular tubes despite normal cell to cell contacts.
LOC101857249 NFX1-type zinc finger-containing protein 1 from Aplysia californica
Aligns to 1797:1851 / 1887 (2.9%), covers 87.0% of PF20173, 76.6 bits
- In search of the Aplysia immunome: an in silico study
Kron, BMC genomics 2022 - “...conserved domains, PANTHER annotation from IPS (PTHR10887:SF470), and homology search, three potential ZNFX1 genes (LOC101862822, LOC101857249, LOC101851452) were identified. Several DNA-damage detecting proteins have also been implicated in cytosolic sensing of viral nucleotide [ 32 , 33 ]. Putative orthologs of several of these, including all...”
- “...IPR013785 4 LOC101856483, LOC101856246, LOC101847430, LOC101847031 ZNFX1 IPR041677, IPR041679, IPR000967, IPR016024 IPR045055, IPR041677, IPR041679 3 LOC101857249, LOC101862822, LOC101851452 RNAi RNA interference (RNAi) is another potent antiviral mechanism of particular importance in arthropods [ 88 ]. Key components of the RISC RNAi complex, including Dicer (LOC101848232), two...”
U3IUP4 Zinc finger NFX1-type containing 1 from Anas platyrhynchos platyrhynchos
Aligns to 2026:2081 / 2110 (2.7%), covers 85.2% of PF20173, 76.0 bits
ZNFX1_HUMAN / Q9P2E3 NFX1-type zinc finger-containing protein 1 from Homo sapiens (Human) (see 3 papers)
Aligns to 1834:1889 / 1918 (2.9%), covers 85.2% of PF20173, 75.3 bits
RN213_HUMAN / Q63HN8 E3 ubiquitin-protein ligase RNF213; ALK lymphoma oligomerization partner on chromosome 17; E3 ubiquitin-lipopolysaccharide ligase RNF213; Mysterin; RING finger protein 213; EC 2.3.2.27; EC 3.6.4.-; EC 2.3.2.- from Homo sapiens (Human) (see 28 papers)
Aligns to 4489:4545 / 5207 (1.1%), covers 85.2% of PF20173, 69.1 bits
- function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PubMed:21799892, PubMed:26126547, PubMed:26278786, PubMed:26766444, PubMed:30705059, PubMed:32139119, PubMed:34012115). Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc- finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (PubMed:34012115). Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains (PubMed:34012115). Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (PubMed:34012115). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (PubMed:30705059). Also regulates lipotoxicity by inhibiting desaturation of fatty acids (PubMed:30846318). Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger: mediates 'Lys-63'-linked ubiquitination of target proteins (PubMed:32139119, PubMed:33842849). Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (PubMed:26766444). Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (PubMed:34012115).
catalytic activity: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
catalytic activity: ATP + H2O = ADP + H(+) + phosphate (RHEA:13065)
subunit: Monomer (By similarity). Interacts with UBE2L3/UBCH7; UBE2L3/UBCH7 is the most efficient ubiquitin-conjugating enzyme E2 for the ubiquitin ligase activity (By similarity). Interacts with UBE2N/UBC13; promoting 'Lys-63'-linked ubiquitination of target proteins (PubMed:32139119, PubMed:33842849).
subunit: (Microbial infection) Interacts with M.tuberculosis protein Rv3655c, which impairs caspase-8 activation and suppresses macrophage apoptosis by blocking the extrinsic pathway. - The Protective Action of Metformin against Pro-Inflammatory Cytokine-Induced Human Islet Cell Damage and the Mechanisms Involved.
Giusti, Cells 2022 - “...Q8N339 METALLOTHIONEIN-1M (PTHR23299:SF50) MT1M 3.11 2.49 Q09666 NEUROBLAST DIFFERENTIATION-ASSOCIATED PROTEIN AHNAK (PTHR23348:SF41) AHNAK 9.48 0.31 Q63HN8 E3 UBIQUITIN-PROTEIN LIGASE RNF213 (PTHR22605:SF16) RNF213 10.35 8.25 O95786 ATP-DEPENDENT RNA HELICASE DDX58-RELATED (PTHR14074:SF16) DDX58 11.21 10.63 P69905 HEMOGLOBIN SUBUNIT ALPHA (PTHR11442:SF48) HBA1 23.19 34.96 Q13113 PDZK1-INTERACTING PROTEIN 1 (PTHR15296:SF0)...”
- Identification of HO-1 as a novel biomarker for graft acute cellular rejection and prognosis prediction after liver transplantation
Jia, Annals of translational medicine 2020 - “...AK1 1.448 Q9GZP4 PITH domain containing 1 PITHD1 1.444 P27348 14-3-3 protein theta YWHAQ 1.436 Q63HN8 Ring finger protein 213 RNF213 1.435 P53004 Biliverdin reductase A BLVRA 1.432 P52907 F-actin-capping protein subunit alpha-1 CAPZA1 1.431 Q99873 Protein arginine methyltransferase 1 PRMT1 1.431 O00151 PDZ and LIM...”
- Proteomic profile of follicular fluid from patients with polycystic ovary syndrome (PCOS) submitted to in vitro fertilization (IVF) compared to oocyte donors
Domingues, JBRA assisted reproduction 2019 - “...NA NA 1 Q53GS7 Nucleoporin GLE1 nucleic acid binding(PC00171);transfer/carrier protein(PC00219) nucleic acid binding(GO:0005488) NA 1 Q63HN8 E3 ubiquitin-protein ligase RNF213 NA NA NA 1 Q6YP21 Kynurenine--oxoglutarate transaminase 3 transaminase(PC00220) transaminase activity(GO:0003824) NA 1 Q70CQ2 Ubiquitin carboxyl-terminal hydrolase 34 NA NA NA 1 Q7Z6M1 Rab9 effector protein...”
- SAMHD1 Enhances Chikungunya and Zika Virus Replication in Human Skin Fibroblasts.
Wichit, International journal of molecular sciences 2019 - “...LAP3 P28838 1.049 Interferon-induced 35 kDa protein IFI35 P80217 1.015 E3 ubiquitin-protein ligase RNF213 RNF213 Q63HN8 0.895 Gamma-interferon-inducible protein 16 IFI16 Q16666 0.878 Double-stranded RNA-activated protein kinase EIF2AK2 * P19525 0.838 HLA class I histocompatibility antigen, A-69 alpha chain HLA-A P10316 0.752 Zika virus infected cells...”
- Comprehensive Proteomics Identification of IFN-λ3-regulated Antiviral Proteins in HBV-transfected Cells.
Makjaroen, Molecular & cellular proteomics : MCP 2018 - Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry.
DeBoer, Virology 2018 - “...+ Yes CYTO. P10155 RO60_HUMAN 0.00874 0.53 + Yes P53350 PLK1_HUMAN 0.01040 0.58 + No Q63HN8 RN213_HUMAN2 0.01317 0.56 + Yes O43747 AP1G1_HUMAN2 0.01982 0.67 + Yes P23284 PPIB_HUMAN 0.02807 0.74 + No P41250 SYG_HUMAN2 0.03898 0.66 + + Yes P23284 PPIB_HUMAN2 0.04214 0.70 + No...”
- Novel and recurrent RNF213 variants in Japanese pediatric patients with moyamoya disease.
Akagawa, Human genome variation 2018 - “...lobe ( i ). Figure 2 ( a ) Protein structure of RNF213, based on Q63HN8 (RN213_HUMAN) in the InterPro ( http://www.ebi.ac.uk/interpro/ ) database. ( b ) DNA sequence chromatograms of the three heterozygous missense variants detected in the present study. ( c ) Multiple alignment...”
- Angiogenic and Immunologic Proteins Identified by Deep Proteomic Profiling of Human Retinal and Choroidal Vascular Endothelial Cells: Potential Targets for New Biologic Drugs.
Smith, American journal of ophthalmology 2018 - “...surface receptor (U-PAR; uPAR) Q03405 3.64 9.3995E-03 Tumstatin Q01955 3.57 5.5203E-03 E3 ubiquitin-protein ligase RNF213 Q63HN8 3.33 2.0305E-23 Diphosphoinositol polyphosphate phosphohydrolase 2 (DIPP-2) Q9NZJ9 3.12 7.1523E-10 Matrix metalloproteinase-14 (MMP-14) P50281 3.12 1.6330E-06 Processed cysteine-rich motor neuron 1 protein Q9NZV1 3.23 2.2781E-07 N-myc-interactor (Nmi) Q13287 3.00 1.6482E-04...”
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NP_001243000 E3 ubiquitin-protein ligase RNF213 isoform 3 from Homo sapiens
Aligns to 4489:4545 / 5207 (1.1%), covers 85.2% of PF20173, 69.1 bits
XP_005257602 E3 ubiquitin-protein ligase RNF213 isoform X1 from Homo sapiens
Aligns to 4538:4594 / 5256 (1.1%), covers 85.2% of PF20173, 69.0 bits
F1M0R1 Ring finger protein 213 from Rattus norvegicus
Aligns to 4415:4469 / 5126 (1.1%), covers 81.5% of PF20173, 66.8 bits
XP_038943469 E3 ubiquitin-protein ligase RNF213 isoform X1 from Rattus norvegicus
Aligns to 4490:4544 / 5201 (1.1%), covers 81.5% of PF20173, 66.8 bits
XP_006534693 E3 ubiquitin-protein ligase RNF213 isoform X1 from Mus musculus
Aligns to 4480:4535 / 5192 (1.1%), covers 83.3% of PF20173, 65.2 bits
- Suppression of RNF213, a susceptibility gene for moyamoya disease, inhibits endoplasmic reticulum stress through SEL1L upregulation.
Ahmed, Biochemical and biophysical research communications 2022 (PubMed)- GeneRIF: Suppression of RNF213, a susceptibility gene for moyamoya disease, inhibits endoplasmic reticulum stress through SEL1L upregulation.
- Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.
Ahel, eLife 2020 - GeneRIF: Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.
- S-nitrosylation of E3 ubiquitin-protein ligase RNF213 alters non-canonical Wnt/Ca+2 signaling in the P301S mouse model of tauopathy.
Amal, Translational psychiatry 2019 - GeneRIF: Study shows that S-nitrosylation of E3 ubiquitin-protein ligase RNF213 alters non-canonical Wnt/Ca+2 signaling in the P301S mouse model of tauopathy.
- Dysregulation of RNF213 promotes cerebral hypoperfusion.
Morimoto, Scientific reports 2018 - GeneRIF: results are indicative of the essential role of RNF213 in the maintenance of cerebral blood flow .
- Transient middle cerebral artery occlusion in mice induces neuronal expression of RNF213, a susceptibility gene for moyamoya disease.
Sato-Maeda, Brain research 2016 (PubMed)- GeneRIF: The Rnf213 gene was up-regulated in the ischemic brain, especially at the penumbra area, as early as 6 h after transient middle cerebral artery occlusion
- Temporal profile of magnetic resonance angiography and decreased ratio of regulatory T cells after immunological adjuvant administration to mice lacking RNF213, a susceptibility gene for moyamoya disease.
Kanoke, Brain research 2016 (PubMed)- GeneRIF: The mechanisms underlying the development of moyamoya disease currently remain unclear, the RNF213 abnormality may compromise immunological self-tolerance, thereby contributing to the development of moyamoya disease.
- PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia.
Banh, Nature cell biology 2016 - GeneRIF: PTP1B/RNF213/alpha-KGDD pathway is critical for survival of HER2(+) breast cancer, and possibly other malignancies, in the hypoxic tumour microenvironment
- Enhanced post-ischemic angiogenesis in mice lacking RNF213; a susceptibility gene for moyamoya disease.
Ito, Brain research 2015 (PubMed)- GeneRIF: Angiogenesis was enhanced in mice lacking RNF213 after chronic hind-limb ischemia, which suggested the potential role of the RNF213 abnormality in the development of pathological vascular networks in chronic ischemia.
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RN213_MOUSE / E9Q555 E3 ubiquitin-protein ligase RNF213; E3 ubiquitin-lipopolysaccharide ligase RNF213; Mysterin; RING finger protein 213; EC 2.3.2.27; EC 3.6.4.-; EC 2.3.2.- from Mus musculus (Mouse) (see 6 papers)
Aligns to 4436:4491 / 5148 (1.1%), covers 83.3% of PF20173, 65.2 bits
- function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PubMed:32573437). Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (PubMed:34012115). Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains (By similarity). Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (By similarity). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (By similarity). Also regulates lipotoxicity by inhibiting desaturation of fatty acids (By similarity). Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger: mediates 'Lys- 63'-linked ubiquitination of target proteins (By similarity). Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (By similarity). Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (PubMed:32573437).
catalytic activity: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
catalytic activity: ATP + H2O = ADP + H(+) + phosphate (RHEA:13065)
subunit: Monomer (PubMed:32573437). Interacts with UBE2L3/UBCH7; UBE2L3/UBCH7 is the most efficient ubiquitin-conjugating enzyme E2 for the ubiquitin ligase activity (PubMed:32573437). Interacts with UBE2N/UBC13; promoting 'Lys-63'-linked ubiquitination of target proteins (By similarity).
disruption phenotype: No visible phenotype (PubMed:23410753, PubMed:24440776). Males and female are fertile and produce normal-sized litters (PubMed:23410753, PubMed:24440776). Mice do not spontaneously develop Moyamoya disease, a chronic occlusive cerebrovascular disease (PubMed:24440776). Mice are however more susceptible to Rift Valley fever virus infection (PubMed:33420513). Mice show elevated expression of MMP9 (PubMed:25383461). - The Proteomic Signature of Intestinal Acute Rejection in the Mouse.
Oltean, Metabolites 2021 - “...2.38 Antimicrobial Inflammatory response Q99KQ4 Nampt Nicotinamide phosphoribosyltransferase 2.12 Cytokine, Glycosyltransferase Cell death and survival E9Q555 Rnf213 E3 ubiquitin-protein ligase RNF213 1.82 Hydrolase, Transferase Angiogenesis P01899 H2-d1 H-2 class I histocompatibility antigen, D-B alpha chain 1.69 Binding protein Immunology Q9R233 Tapbp Tapasin 1.59 Binding protein Immunology...”
- Quantitative Proteomics Reveals Docosahexaenoic Acid-Mediated Neuroprotective Effects in Lipopolysaccharide-Stimulated Microglial Cells
Yang, Journal of proteome research 2020 - “...cell nuclear differentiation antigen-like protein MNDAL 2.67 n.d. 3.25 Q64337 sequestosome-1 SQSTM1 1.86 0.22 2.02 E9Q555 E3 ubiquitin-protein ligase RNF213 1.66 2.01 2.03 Q8VI94 2-5-oligoadenylate synthase-likeprotein1 OASL1 1.54 1 1.94 Increased in DHA, Lower/Unchanged in LPS Q3UH60 disco-interacting protein 2-B DIP2B n.d. 2.43* n.d. Q91W86 vacuolar...”
- iTRAQ‑based proteomic analysis of endotoxin tolerance induced by lipopolysaccharide
Zhang, Molecular medicine reports 2019 - “...1.363 20 Q9Z0J0 Npc2 Epididymal secretory protein E1 1.362 21 Q9JHK5 Plek Pleckstrin 1.336 22 E9Q555 Rnf213 E3 ubiquitin-protein ligase RNF213 1.328 23 D0QMC3 Mndal Myeloid cell nuclear differentiation antigen-like protein 1.327 24 P20108 Prdx3 Thioredoxin-dependent peroxide reductase, mitochondrial 1.304 25 P97429 Anxa4 Annexin A4 1.298...”
- The ubiquitin ligase Cullin5SOCS2 regulates NDR1/STK38 stability and NF-κB transactivation
Paul, Scientific reports 2017 - “...3.285 1.189 12 169.43 Q8BW84 Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial Pet112 3.543 1.180 5 61.509 E9Q555 E3 ubiquitin-protein ligase RNF213 Rnf213 2.675 1.138 18 584.49 Q8R5C5 Beta-centractin Actr1b 1.997 1.098 6 42.281 Q9DCJ5 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Ndufa8 3.935 1.098 5 19.992...”
- iTRAQ protein profile analysis of neuroblastoma (NA) cells infected with the rabies viruses rHep-Flury and Hep-dG.
Yang, Frontiers in microbiology 2015 - “...3 1.375 0.026 Ddx58 protein Ddx58 A1L0V6 2 1.378 0.034 E3 ubiquitin-protein ligase RNF213 Rnf213 E9Q555 16 1.391 0.044 Signal transducer and activator of transcription 3 Stat3 Q6GU23 2 1.415 0.017 mRNA H2-D1 Q61892 1 1.454 0.046 Discoidin domain-containing receptor 2 Ddr2 Q62371 2 1.490 0.053...”
- Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway.
Zhong, Proteomics 2015 - “...1.7 Ranbp3 Ran-binding protein 3 Q9CT10 S283 2.4 T56 2.4 Rnf213 E3 ubiquitin-protein ligase RNF213 E9Q555 T46 3.3 Rragc Ras-related GTP-binding protein C Q99K70 S94 1.9 T95 2.1 Sept9 Septin-9 Q80UG5 S30 1.8 Snrnp70 U1 small nuclear ribonucleoprotein 70 kDa Q62376 S408 7.6 Trim28 Transcription intermediary...”
- Comparative proteomic analysis identifies age-dependent increases in the abundance of specific proteins after deletion of the small heat shock proteins αA- and αB-crystallin.
Andley, Biochemistry 2013 - “...6171 catenin -1, Q02248 8 85 1.12 3.27 3.65 MCG140784, Q792Z1 4 26 Uncharacterized protein, E9Q555 4 584 -1-antitrypsin 14, Q00897 3 46 Nesprin-1, Q6ZWR6 3 1010 Liver carboxylesterase N, P23953 3 61 Alpha-2-macroglobulin, Q61838 2 166 Glutamate dehydrogenase 1, mitochondrial P26443 2 61 6278 Histone...”
DDB_G0278761 NF-X1-type zinc finger-containing protein from Dictyostelium discoideum AX4
Aligns to 1718:1772 / 1793 (3.1%), covers 96.3% of PF20173, 64.3 bits
E1BHN4 Ring finger protein 213 from Bos taurus
Aligns to 4303:4358 / 5015 (1.1%), covers 85.2% of PF20173, 63.6 bits
R213B_DANRE / A0A0R4I9Y1 E3 ubiquitin-protein ligase rnf213-beta; E3 ubiquitin-lipopolysaccharide ligase rnf213-beta; Mysterin-B; Mysterin-beta; RING finger protein 213-B; RING finger protein 213-beta; EC 2.3.2.27; EC 3.6.4.-; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see 2 papers)
Aligns to 4436:4491 / 5061 (1.1%), covers 85.2% of PF20173, 57.2 bits
- function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity. Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ- type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS. Ubiquitination of LPS triggers cell- autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (By similarity). Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (By similarity). Also regulates lipotoxicity by inhibiting desaturation of fatty acids. Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger. Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of proteins downstream of rspo3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression. Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (By similarity).
catalytic activity: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
catalytic activity: ATP + H2O = ADP + H(+) + phosphate (RHEA:13065)
disruption phenotype: No visible phenotype (PubMed:21799892, PubMed:26530008). No vascular phenotype (PubMed:21799892, PubMed:26530008).
Or search for genetic data about PF20173 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory