Family Search for TIGR00110
TIGR00110 hits 142 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
HI0738 dihydroxyacid dehydratase (ilvD) from Haemophilus influenzae Rd KW20
Aligns to 17:608 / 612 (96.7%), covers 99.6% of TIGR00110, 912.6 bits
IlvD / b3771 dihydroxy-acid dehydratase (EC 4.2.1.9) from Escherichia coli K-12 substr. MG1655 (see paper)
ilvD / P05791 dihydroxy-acid dehydratase (EC 4.2.1.9) from Escherichia coli (strain K12) (see 14 papers)
ILVD_ECOLI / P05791 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Escherichia coli (strain K12) (see 4 papers)
ilvD / EW|b3771 dihydroxyacid dehydratase from Escherichia coli K12 (see 2 papers)
YP_026248 dihydroxy-acid dehydratase from Escherichia coli str. K-12 substr. MG1655
b3771 dihydroxy-acid dehydratase from Escherichia coli str. K-12 substr. MG1655
Aligns to 17:611 / 616 (96.6%), covers 99.6% of TIGR00110, 908.4 bits
- function: Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo- 3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3- dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.
catalytic activity: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O (RHEA:24809)
catalytic activity: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2- oxopentanoate + H2O (RHEA:27694)
cofactor: [2Fe-2S] cluster (Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.)
cofactor: Mg(2+)
subunit: Homodimer. - Integrated network analysis identifies nitric oxide response networks and dihydroxyacid dehydratase as a crucial target in Escherichia coli.
Hyduke, Proceedings of the National Academy of Sciences of the United States of America 2007 - GeneRIF: This approach identified the comprehensive E. coli NO response network and evinced that nitric oxide (NO) halts bacterial growth via inhibition of the branched-chain amino acid biosynthesis enzyme dihydroxyacid dehydratase.
- The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase.
Flint, The Journal of biological chemistry 1993 (PubMed)- GeneRIF: N-terminus verified by Edman degradation on mature peptide
- Protein-based biorefining driven by nitrogen-responsive transcriptional machinery
Ma, Biotechnology for biofuels 2020 - “...dihydroxy acid dehydratase are encoded by alsS (UniProt: Q04789), ilvC (UniProt: P05793), and ilvD (UniProt: P05791), respectively, together, these enzymes convert pyruvate to 2-ketoisovalerate (KIV) and 2-keto-3-methyl-valerate (KMV), which are the direct precursors of valine and isoleucine, respectively. A substantial proportion of the produced KIV, KMV,...”
- A Multiplex Enzymatic Machinery for Cellular Protein S-nitrosylation
Seth, Molecular cell 2018 - “...P0AG67 2.6 1.9 1.4 HybC Hydrogenase-2 large chain P0ACE0 2.4 1.9 1.3 IlvD Dihydroxy-acid dehydratase P05791 4.0 3.3 1.2 NirB Nitrite reductase (NADH) large subunit P08201 3.1 2.6 1.2 Y Hcr NADH oxidoreductase HCR P75824 3.3 2.7 1.2 Y Table 2 GAPDH-dependent S-nitrosylation of Proteins Proteins...”
- Characterization of D-xylonate dehydratase YjhG from Escherichia coli
Jiang, Bioengineered 2015 - “...accession numbers are as follows: E. coli (ILvD), P05791; E. coli (EDD), AAB59053; Z. mobilis, P21909. sequences with several IlvD/EDD proteins demonstrated...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...(DIGE 4.5-6.5) 5.29/51,264 (DIGE 4.5-6.5) IlvD P05791 Dihydroxy-acid dehydratase 5.59/65,400.37 5.54/62,065 (DIGE 4.5-6.5) IlvE P0AB80 Branched-chain amino acid...”
- Genome-scale analysis to the impact of gene deletion on the metabolism of E. coli: constraint-based simulation approach
Xu, BMC bioinformatics 2009 - “...b0159 b0074 b0088 b0182 b2472 b0173, b0174, b0369 b3770 b0090 b0524 b2478 b0414, b0415, b0417 b3771 b0091 b0914 b2838 b0420, b0421, b0423 b3774 b0954 b0915 b3359 b0475, b0750, b0907 b1093 b0918 b3433 b1096, b1208, b1210 b1094 b1094 b3809 b1277, b1662, b1740 b1288 b1215 s0001 b1812, b2103,...”
- Experimental and computational assessment of conditionally essential genes in Escherichia coli
Joyce, Journal of bacteriology 2006 - “...hisI (b2026) ilvA (b3772) ilvB (b3671) ilvC (b3774) ilvD (b3771) leuA (b0074) leuB (b0073) leuC (b0072) leuD (b0071) lysA (b2838) metA (b4013) metB (b3939) metC...”
- Combined, functional genomic-biochemical approach to intermediary metabolism: interaction of acivicin, a glutamine amidotransferase inhibitor, with Escherichia coli K-12
Smulski, Journal of bacteriology 2001 - “...b2507 b2508 b1615 b2231 b1619 b4348 b4018 b3671 b3774 b3771 b3770 b3767 b3769 b3168 b0275 b0274 b0051 b2568 b0072 b0071 b3455 b3460 b3458 b4024 b4033 b1620...”
KPN_04270 dihydroxy-acid dehydratase from Klebsiella pneumoniae subsp. pneumoniae MGH 78578
A6TGF8 Dihydroxy-acid dehydratase from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Aligns to 17:611 / 616 (96.6%), covers 99.6% of TIGR00110, 908.0 bits
ECs4705 dihydroxyacid dehydratase from Escherichia coli O157:H7 str. Sakai
Aligns to 17:611 / 616 (96.6%), covers 99.6% of TIGR00110, 907.1 bits
SC3809 dihydroxyacid dehydratase from Salmonella enterica subsp. enterica serovar Choleraesuis str. SC-B67
Aligns to 17:611 / 616 (96.6%), covers 99.6% of TIGR00110, 901.2 bits
- FlbT couples flagellum assembly to gene expression in Caulobacter crescentus
Mangan, Journal of bacteriology 1999 - “...SC3802 SC3803 SC3804 SC3805 SC3806 SC3807 SC3808 SC3809 SC3843 Plasmids pJBZ282 pfljK::lacZ pfljK1::lacZ pfljK2::lacZ pfljK3::lacZ pfljK-lacZ/290 This work This...”
- “...Transduction of SC508 to Kanr recA Phage SC3809 SC276 fliL::Tn5 str-140 flbT650 fliL179::Tn5 flbT650 flhA608::Tn5 flbT650 flaN176::Tn5 flbT650 flbD198::Tn5...”
- A new class of Caulobacter crescentus flagellar genes
Leclerc, Journal of bacteriology 1998 - “...SC1117 SC1127 SC1128 SC1132 SC1134 SC1135 SC2663 SC3090 SC3809 SC3898 SC3899 SC3971 SC3973 SC3975 SC4016 SC4250 Wild type syn-1000 bla-6 syn-1000 ctrA401...”
- “...flmE::cat flmG::cat SC1029 SC1032 SC1055 SC1066 SC1132 SC2663 SC3809 SC1117 SC1134 SC1135 SC1128 flhB (II) flbD (II) rpoN (II) fliL (II) flhA (II) fliM...”
ilvDD / O27498 dihydroxy-acid dehydratase subunit (EC 4.2.1.9) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (see 4 papers)
Aligns to 14:549 / 549 (97.6%), covers 99.8% of TIGR00110, 882.3 bits
MS2219 dihydroxy-acid dehydratase from [Mannheimia] succiniciproducens MBEL55E
Aligns to 17:608 / 611 (96.9%), covers 99.6% of TIGR00110, 879.9 bits
MS2219 IlvD protein from Mannheimia succiniciproducens MBEL55E
Aligns to 21:612 / 615 (96.3%), covers 99.6% of TIGR00110, 879.9 bits
PMI3302 dihydroxy-acid dehydratase from Proteus mirabilis HI4320
PMI_RS16415 dihydroxy-acid dehydratase from Proteus mirabilis HI4320
Aligns to 17:611 / 616 (96.6%), covers 99.6% of TIGR00110, 875.9 bits
- Organ agar serves as physiologically relevant alternative for in vivo bacterial colonization
Pearson, Infection and immunity 2023 - “...PMI3301 ilvE PMI_RS16410 207 B4 1.016 U Branched-chain amino acid aminotransferase Amino acid metabolism Y PMI3302 ilvD PMI_RS16415 207 D2 0.913 U Dihydroxy-acid dehydratase Amino acid metabolism n/a PMI3316 wecA/ rfe PMI_RS16480 219 D6 0.351 U Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase Lipopolysaccharide biosynthesis Y PMI3319 rffD PMI_RS16495...”
- Pathogenesis of Proteus mirabilis Infection
Armbruster, EcoSal Plus 2018 - “...efflux protein Y ( 129 ) pldA PMI3344 Phospholipase A N ( 129 ) ilvD PMI3302 Dihydroxy-acid dehydratase (branched chain amino acid biosynthesis protein) Y f ( 129 ) lon PMI0117 ATP-dependent proteinase, serine peptidase Y ( 129 ) argR PMI3399 Transcriptional regulator, repressor of the...”
- Organ agar serves as physiologically relevant alternative for in vivo bacterial colonization
Pearson, Infection and immunity 2023 - “...PMI_RS16410 207 B4 1.016 U Branched-chain amino acid aminotransferase Amino acid metabolism Y PMI3302 ilvD PMI_RS16415 207 D2 0.913 U Dihydroxy-acid dehydratase Amino acid metabolism n/a PMI3316 wecA/ rfe PMI_RS16480 219 D6 0.351 U Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase Lipopolysaccharide biosynthesis Y PMI3319 rffD PMI_RS16495 219 C5...”
APL_0097 Dihydroxy-acid dehydratase from Actinobacillus pleuropneumoniae L20
Aligns to 17:608 / 611 (96.9%), covers 99.6% of TIGR00110, 874.3 bits
- Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae
Labrie, Veterinary research 2010 - “...APL_0099 ilvG Acetolactate synthase isozyme II large subunit (AHAS-II) 3.915 APL_1499 thrC Threonine synthase 3.198 APL_0097 ilvD Dihydroxy-acid dehydratase 3.142 APL_0393 leuA 2-isopropylmalate synthase 3.000 APL_0098 ilvM Acetolactate synthase isozyme II small subunit (AHAS-II) 2.934 APL_2027 hisF Imidazole glycerol phosphate synthase subunit hisF 2.833 APL_0702 serC...”
VC0028 dihydroxy-acid dehydratase from Vibrio cholerae O1 biovar eltor str. N16961
Aligns to 17:608 / 613 (96.6%), covers 99.6% of TIGR00110, 874.3 bits
- Transcriptomics reveals a cross-modulatory effect between riboflavin and iron and outlines responses to riboflavin biosynthesis and uptake in Vibrio cholerae
Sepúlveda-Cisternas, Scientific reports 2018 - “...portion 1.809 VC0018 ibpA 16kDa heat shock protein A 1.740 VC0027 threonine dehydratase 1.226 1.191 VC0028 dihydroxy-acid dehydratase 1.087 VC0030 ilvM acetolactate synthase II small subunit 1.116 VC0053 hypothetical protein 1.074 VC0089 cytochrome c551 peroxidase 1.076 VC0102 hypothetical protein 1.160 VC0138 hypothetical protein 1.564 VC0139 DPS...”
- Pyomelanin produced by Vibrio cholerae confers resistance to predation by Acanthamoeba castellanii
Noorian, FEMS microbiology ecology 2017 - “...VC2643, and VC2508 (arginine metabolism and biosynthesis), VC1704 (cysteine and methionine metabolism), VC0162, VC0031 and VC0028 (isoleucine biosynthesis) and VC0392, VCA0604 and VCA0605 (aminotransferases). The increase in metabolism and energy production might be related to an increase in available nutrient resources since feeding will result in...”
- Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak
Haley, PloS one 2014 - “...A A CDP-diacylglycerolserine O-phosphatidyltransferase VC1899 C C C C T C C C hypothetical protein VC0028 C C C C C C T C Dihydroxy-acid dehydratase VC0031 C C C C C C C A Acetolactate synthase large subunit VC1359 T T T T C C...”
- Host-induced epidemic spread of the cholera bacterium
Merrell, Nature 2002 - “...genes differentially expressed in the stool samples are VC0028, VC0941, VC0869, VC0051, VC0647, VC0468, VC2350 and VCA0583, all of which were recently...”
HSM_0213 dihydroxy-acid dehydratase from Haemophilus somnus 2336
HSM_0213 dihydroxy-acid dehydratase from Histophilus somni 2336
Aligns to 17:608 / 611 (96.9%), covers 99.6% of TIGR00110, 870.2 bits
NJ56_05450 dihydroxy-acid dehydratase from Yersinia ruckeri
Aligns to 17:611 / 616 (96.6%), covers 99.6% of TIGR00110, 870.1 bits
- Genome Sequence of the Fish Pathogen Yersinia ruckeri SC09 Provides Insights into Niche Adaptation and Pathogenic Mechanism
Liu, International journal of molecular sciences 2016 - “...transport exists exclusively in bacteria [ 58 ]. PTS for transport and metabolism of N-acetyl-glucosamine (NJ56_05450), sucrose (NJ56_16635), cellobiose (NJ56_09805, NJ56_09815-20), mannitol (NJ56_13600), ascorbate (NJ56_08600-05, NJ56_15455-65), glucose (NJ56_07070, NJ56_15910), fructose (NJ56_15025-35), nitrogen (NJ56_11960, NJ56_11970, NJ56_10665), mannose (NJ56_09565-75), trehalose (NJ56_11995, NJ56_15910), glucitol/sorbitol (NJ56_11140-50), and N -acetylga-lactosamine (NJ56_02695-10)...”
VF_2559 dihydroxy-acid dehydratase from Vibrio fischeri ES114
Aligns to 17:608 / 613 (96.6%), covers 99.6% of TIGR00110, 866.8 bits
GYMC52_2001 dihydroxy-acid dehydratase from Geobacillus sp. Y412MC52
Aligns to 17:559 / 559 (97.1%), covers 99.8% of TIGR00110, 866.8 bits
EAMY_0161 dihydroxy-acid dehydratase from Erwinia amylovora CFBP1430
Aligns to 17:611 / 616 (96.6%), covers 99.6% of TIGR00110, 864.9 bits
WP_010874288 dihydroxy-acid dehydratase from Synechocystis sp. PCC 6803
slr0452 dihydroxyacid dehydratase from Synechocystis sp. PCC 6803
Aligns to 18:558 / 561 (96.4%), covers 99.8% of TIGR00110, 852.3 bits
- Plastid ancestors lacked a complete Entner-Doudoroff pathway, limiting plants to glycolysis and the pentose phosphate pathway
Evans, Nature communications 2024 - “...KDPG 28 , 48 , 49 . Chen et al. have speculated that Synechocystis DHAD (WP_010874288 or slr0452 ) might dehydrate gluconate to KDG, followed by phosphorylation to KDPG through the semi-phosphorylated pathway 11 . Our analysis indicates that Synechocystis PCC 6803, like the majority of...”
- Plastid ancestors lacked a complete Entner-Doudoroff pathway, limiting plants to glycolysis and the pentose phosphate pathway
Evans, Nature communications 2024 - “..., 48 , 49 . Chen et al. have speculated that Synechocystis DHAD (WP_010874288 or slr0452 ) might dehydrate gluconate to KDG, followed by phosphorylation to KDPG through the semi-phosphorylated pathway 11 . Our analysis indicates that Synechocystis PCC 6803, like the majority of cyanobacteria, does...”
- Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803
Xie, Biotechnology for biofuels and bioproducts 2023 - “...native acetolactate synthase (AlsS); Slr2088, large subunit of native AlsS; Sll1363, native acetohydroxy-acid isomeroreductase (IlvC); Slr0452, native dihydroxy-acid dehydratase (IlvD); LeuA, 2-isopropylmalate synthase; LeuCD, 3-isopropylmalate dehydratase; LeuB, 3-isopropylmalate dehydrogenase; Kivd S286T , -ketoisovalerate decarboxylase ( Lactococcus lactis ); Slr1192 OP , codon-optimized native alcohol dehydrogenase. Dotted...”
- “...of gene fragments kivd S286T , slr1192 OP , sll0065 , slr2088 , sll1363 , slr0452 , alsS , ilvC , ilvD , fbaA , tktA and pyk1 were codon-optimized and synthesized by GenScript. All gene sequences and all primers used for plasmid construction are listed...”
- Site-2 Protease Slr1821 Regulates Carbon/Nitrogen Homeostasis during Ammonium Stress Acclimation in Cyanobacterium Synechocystis sp. PCC 6803
Lin, International journal of molecular sciences 2023 - “...well as the downregulation of the gene for the ED pathway enzyme phosphogluconate dehydratase ( slr0452 ). Disruption of the OPP pathway was implied as the upregulation of the gene for 6-phosphogluconate dehydrogenase ( sll0329 ) and the downregulation of the gene for transketolase ( sll1070...”
- Entner-Doudoroff pathway in Synechocystis PCC 6803: Proposed regulatory roles and enzyme multifunctionalities
Bachhar, Frontiers in microbiology 2022 - “...route, consisting of only two reactions. The first one is catalyzed by 6-phosphogluconate dehydratase (EDD, slr0452 , currently annotated as ilvD dihydroxy-acid dehydratase), producing 2-keto3-deoxygluconate-6-phosphate (KDPG). The second reaction is catalyzed by keto3-deoxygluconate-6-phosphate aldolase (EDA, sll0107 ), producing pyruvate and glyceraldehyde 3-phosphate ( Figure 1 ,...”
- Enhancement of photosynthetic isobutanol production in engineered cells of Synechocystis PCC 6803
Miao, Biotechnology for biofuels 2018 - “...all the examined strains (Fig. 3 b). In addition, the expression of sll0065, slr2088, and slr0452 could not be detected using anti-Flag tag western immunoblot. Thus, two hypotheses could be made: (i) there is no other bottleneck(s) than Kivd in the isobutanol synthesis pathway. Thus, the...”
- The Entner-Doudoroff pathway is an overlooked glycolytic route in cyanobacteria and plants
Chen, Proceedings of the National Academy of Sciences of the United States of America 2016 - “...the ED pathway uncovered candidates for 6PG-dehydratase (edd, slr0452) and the key enzyme KDPG aldolase (eda, sll0107) (Table S1). The candidate KDPG aldolase...”
- “...2D). In contrast to eda (sll0107), the gene edd (slr0452) could not be completely deleted from all genome copies of Synechocystis (Fig. S2F). We hypothesize...”
- Expression profiling of the bloom-forming cyanobacterium Nodularia CCY9414 under light and oxidative stress conditions
Kopf, The ISME journal 2015 - “...59) None Ssl3364 (2e 21) Sll0877 (2e 77) Sll1937 (2e 06) Slr0452 (0.0) Sll1154 (e 112) Sll0772 (e 98) None Sll0549 (e 73) Slr2073 (3e 49) Slr1391 (e 16) None...”
- Global transcriptional profiles of the copper responses in the cyanobacterium Synechocystis sp. PCC 6803
Giner-Lamia, PloS one 2014 - “...thrA 0.37 Homoserine dehydrogenase sll0461 proA 0.35 Gamma-glutamyl phosphate reductase sll1760 thrB 0.31 Homoserine kinase slr0452 ilvD 0.29 Dihydroxyacid dehydratase sll0080 argC 0.28 N-acetyl-gamma-glutamyl-phosphate reductase sll0450 norB 0.12 Cytochrome b subunit of nitric oxide reductase Transport and binding proteins sll0064 0.18 Putative polar amino acid transport...”
NP_386934 PROBABLE DIHYDROXY-ACID DEHYDRATASE PROTEIN from Sinorhizobium meliloti 1021
Aligns to 17:609 / 612 (96.9%), covers 99.6% of TIGR00110, 850.0 bits
BCAM1262 dihydroxyacid dehydratase from Burkholderia cenocepacia J2315
Aligns to 17:614 / 619 (96.6%), covers 99.6% of TIGR00110, 848.1 bits
AB395_RS15445 dihydroxy-acid dehydratase from Sinorhizobium fredii CCBAU 45436
Aligns to 17:609 / 612 (96.9%), covers 99.6% of TIGR00110, 847.1 bits
RHE_RS08720 dihydroxy-acid dehydratase from Rhizobium etli CFN 42
Aligns to 17:609 / 612 (96.9%), covers 99.6% of TIGR00110, 846.1 bits
- Rhizobium etli CFN42 proteomes showed isoenzymes in free-living and symbiosis with a different transcriptional regulation inferred from a transcriptional regulatory network
Taboada-Castro, Frontiers in microbiology 2022 - “...6, and Supplementary Table 3A ), the enzyme ilvD , dihydroxy-acid dehydratase [EC:4.2.1.9], and the RHE_RS08720 and RHE_RS23070 proteins were expressed in MM and bacteroid, respectively, supporting multiplicity ( Table 1 ). Similarly, for valine, leucine, and isoleucine degradation ( Supplementary Table 2 pathway 7, and...”
ILVD_RHIJ3 / Q1MIB2 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Rhizobium johnstonii (strain DSM 114642 / LMG 32736 / 3841) (Rhizobium leguminosarum bv. viciae) (see paper)
RL1803 putative dihydroxy-acid dehydratase from Rhizobium leguminosarum bv. viciae 3841
Aligns to 17:609 / 612 (96.9%), covers 99.6% of TIGR00110, 845.0 bits
- function: Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo- 3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3- dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.
catalytic activity: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O (RHEA:24809)
catalytic activity: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2- oxopentanoate + H2O (RHEA:27694)
cofactor: [2Fe-2S] cluster (Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.)
cofactor: Mg(2+)
subunit: Homodimer.
disruption phenotype: Does not nodulate the roots of pea plants. - Lifestyle adaptations of Rhizobium from rhizosphere to symbiosis
Wheatley, Proceedings of the National Academy of Sciences of the United States of America 2020 (secret)
DR1132, DR_1132 dihydroxy-acid dehydratase from Deinococcus radiodurans R1
Aligns to 17:607 / 607 (97.4%), covers 99.6% of TIGR00110, 843.0 bits
ACIAD1266 dihydroxy-acid dehydratase from Acinetobacter sp. ADP1
Aligns to 17:609 / 609 (97.4%), covers 99.4% of TIGR00110, 842.1 bits
ABO_2312 dihydroxy-acid dehydratase from Alcanivorax borkumensis SK2
Aligns to 69:662 / 667 (89.1%), covers 99.6% of TIGR00110, 842.1 bits
Cthe_2713 dihydroxy-acid dehydratase from Clostridium thermocellum ATCC 27405
Aligns to 14:553 / 554 (97.5%), covers 99.8% of TIGR00110, 840.9 bits
Atu1918 dihydroxy-acid dehydratase from Agrobacterium tumefaciens str. C58 (Cereon)
Aligns to 17:609 / 611 (97.1%), covers 99.4% of TIGR00110, 838.9 bits
IYO_025960 dihydroxy-acid dehydratase from Pseudomonas syringae pv. actinidiae ICMP 18884
Aligns to 17:607 / 615 (96.1%), covers 99.6% of TIGR00110, 838.3 bits
- Transposon insertion libraries for the characterization of mutants from the kiwifruit pathogen Pseudomonas syringae pv. actinidiae
Mesarich, PloS one 2017 - “...in Fig 2B . Of the 13 genes identified, IYO_007210 , IYO_008330 , IYO_008720 and IYO_025960 , which putatively encode the phosphoribosylformylglycinamidine synthase enzyme PurL (purine biosynthesis), the quinolinate synthase enzyme NadA (NAD biosynthesis), the phosphoribosylglycinamide formyltransferase enzyme PurN (purine biosynthesis), and the dihydroxy-acid dehydratase enzyme...”
- “...red arrows at positions P96-A9, P96-B10 and P96-G2, respectively; (B) Schematic of the IYO_007210 , IYO_025960 and IYO_025885 genes disrupted in the auxotrophic mutants retrieved from positions P96-A9, P96-B10 and P96-G2, respectively in (A). Arrow colors correspond to the mutants highlighted in (A), and show the...”
HD73_2029 dihydroxy-acid dehydratase from Bacillus thuringiensis serovar kurstaki str. HD73
Aligns to 14:553 / 557 (96.9%), covers 99.8% of TIGR00110, 838.2 bits
ILV3_YEAST / P39522 Dihydroxy-acid dehydratase, mitochondrial; DAD; EC 4.2.1.9 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 13 papers)
NP_012550, YJR016C Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids from Saccharomyces cerevisiae
Aligns to 38:585 / 585 (93.7%), covers 99.6% of TIGR00110, 838.2 bits
- function: Dihydroxyacid dehydratase that catalyzes the third step in the common pathway leading to biosynthesis of branched-chain amino acids (PubMed:20008079, PubMed:21798060, PubMed:21987576, PubMed:22954227, PubMed:25280745, PubMed:26543501, PubMed:27532773, PubMed:28505306, PubMed:30850698, PubMed:31303893, PubMed:33620449, PubMed:8299945). Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3- methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3- methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3- methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L- valine, respectively (PubMed:20008079, PubMed:27532773). Required for the synthesis of alpha-isopropylmalate which modulates the activity of LEU3 and subsequently regulates the expression of LEU1 (PubMed:20008079).
catalytic activity: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O (RHEA:24809)
catalytic activity: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2- oxopentanoate + H2O (RHEA:27694)
cofactor: [2Fe-2S] cluster (Binds 1 [2Fe-2S] cluster per subunit.)
cofactor: Mg(2+)
disruption phenotype: Strongly reduces the iron responsiveness of expression of LEU1 by affecting the synthesis of alpha-isopropylmalate. - Combinatorial library design for improving isobutanol production in Saccharomyces cerevisiae
Gambacorta, Frontiers in bioengineering and biotechnology 2022 - “...ID: A0A0G9FA99), KARI from Pfam families PF01450 and PF07991, DHAD from S. cerevisiae (Uniprot ID: P39522), KDC from S. cerevisiae (Uniprot ID: Q06408), and ADH from S. cerevisiae (Uniprot ID: P38113). A diverse set of variants was chosen from the resulting network by 1) gathering homologs...”
- Identification of in vivo substrates of the yeast mitochondrial chaperonins reveals overlapping but non-identical requirement for hsp60 and hsp10
Dubaquié, The EMBO journal 1998 - “...RHO1 HSP60 SSC1 2,3-dihydroxy acid hydrolase P39522 ketol-acid reductoisomerase P06168 isocitrate dehydrogenase P28834 aconitase homolog P39533 aconitase P19414...”
- Commonalities and Differences in the Transcriptional Response of the Model Fungus Saccharomyces cerevisiae to Different Commercial Graphene Oxide Materials
Laguna-Teno, Frontiers in microbiology 2020 - “...in the pathway ( Ihrig et al., 2010 ). One of them, the dihydroxyacid dehydratase YJR016C ( ILV3 ), which catalyzes the third step in the common pathway leading to biosynthesis of leucine, isoleucine and valine, was downregulated upon exposure to both nanomaterials. The transcriptional changes...”
- tRNA Genes Affect Chromosome Structure and Function via Local Effects
Hamdani, Molecular and cellular biology 2019 - “...YER073w YER091c YGL009c YHR208w YJR010w YJR016c 0.030966243 0.039894621 0.015662463 0.045197971 0.042003857 0.009117145 0.007776891 0.042003857 0.017736878...”
- Transcriptional profiling reveals molecular basis and novel genetic targets for improved resistance to multiple fermentation inhibitors in Saccharomyces cerevisiae
Chen, Biotechnology for biofuels 2016 - “...YNL160W, YOL058W, YOL086C, YOR184W, YOR375C YCL030C, YDR007W, YDR502C , YER091C , YGL245W, YGL256W , YJL200C, YJR016C, YLR142W , YMR169C , YMR250W YCL030C, YCR005C, YDL168W, YDL182W, YDR007W, YDR135C, YDR502C , YDR513W, YEL046C, YER043C, YER091C , YFL030W, YGL009C, YGL256W , YGR209C, YGR267C, YIL074C, YJL101C, YJR010W, YJR137C, YJR139C, YKL001C,...”
- Systematic chemical-genetic and chemical-chemical interaction datasets for prediction of compound synergism
Wildenhain, Scientific data 2016 - “...human familial dysautonomia (FD) protein Null mutant is viable, insensitive tokiller toxin, G1 delay S000004376 YJR016C ILV3 + Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Null mutant is viable, isoleucine and valine auxotroph S000003777 YPL209C IPL1 +...”
- Genome-Wide Transcription Study of Cryptococcus neoformans H99 Clinical Strain versus Environmental Strains
Movahed, PloS one 2015 - “...3.0 3.2 2.6 0.0002 CNAG_00484T0_1473 2.9 3.0 2.4 0.0186 CNAG_04346T0_968 2.9 3.4 2.9 0.0076 CNAG_06540T1_1679 NP_012550 ILV3 Dihydroxy-acid dehydratase, mitochondrial 2.8 2.0 3.9 0.0038 CNAG_04122T0_1086 NP_013755 ARA2 D-arabinose 1-dehydrogenase 2.7 2.7 2.4 0.0100 CNAG_02336T0_1615 NP_013031 VBA5 Vacuolar basic amino acid transporter 5 2.6 2.6 4.7 0.0096...”
- Deletion of a subgroup of ribosome-related genes minimizes hypoxia-induced changes and confers hypoxia tolerance
Shah, Physiological genomics 2011 - “...gi 809587 YGL234W YGL156W YAL038W YNL118C YHR132C YMR108W YJR016C YKL103C YGL009C YBL091C YGR240C YDL185W YPR074C YOL057W YPR022C ADE5,7 AMS1 CDC19 DCP2 ECM14...”
- Comparative transcriptomic and proteomic profiling of industrial wine yeast strains
Rossouw, Applied and environmental microbiology 2010 - “...MCR1 MDH1 MET10 MET17 MET22 MET3 MET6 YMR108W YJR016C YLR355C YCL009C YHR216W YLR432W YML056C YBR011C YCL018W YNL104C YFL018C YIR034C YIL094C YDL131W YDR234W...”
- “...YDR035W YBR249C YPR145W YHR208W YOR375C YDL022W YPR160W YJR016C YLR355C YCL018W YIL094C YDL131W YDR234W YNR050C YLR044C YGR240C YMR205C YMR105C YDR502C YLR058C...”
- Combined analysis of expression data and transcription factor binding sites in the yeast genome
Nagaraj, BMC genomics 2004 - “...ALD6 0.597 0.018 0.009 - YBR028C 0.793 0.046 0.036 - YOL022C 0.563 0.023 0.013 - YJR016C ILV3 0.248 0.025 0.006 - YBR218C PYC2 0.324 0.027 0.008 - YFR040W SAP155 0.234 0.165 0.038 - YMR269W 0.332 0.049 0.016 - YJL129C TRK1 0.297 0.083 0.024 - YCR053W THR4...”
- More
A1S_3455 dihydroxy-acid dehydratase from Acinetobacter baumannii ATCC 17978
Aligns to 3:595 / 595 (99.7%), covers 99.4% of TIGR00110, 837.9 bits
Q2YNW9 Dihydroxy-acid dehydratase from Brucella abortus (strain 2308)
BAB1_0096 Dihydroxy-acid and 6-phosphogluconate dehydratase:Dihydroxy-acid dehydratase from Brucella melitensis biovar Abortus 2308
Aligns to 17:609 / 611 (97.1%), covers 99.6% of TIGR00110, 837.8 bits
GBAA_1853 dihydroxy-acid dehydratase from Bacillus anthracis str. 'Ames Ancestor'
BAS1717 dihydroxy-acid dehydratase from Bacillus anthracis str. Sterne
Aligns to 14:553 / 557 (96.9%), covers 99.8% of TIGR00110, 837.5 bits
BMEI1848 DIHYDROXY-ACID DEHYDRATASE from Brucella melitensis 16M
Aligns to 19:611 / 613 (96.7%), covers 99.6% of TIGR00110, 837.5 bits
- Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics
He, Frontiers in cellular and infection microbiology 2012 - “...macrophages, HeLa 14979322 47 gluP BMEII1053 Mice 12414147 48 gnd BMEII1124 Mice 12761078 49 ilvD BMEI1848 Mice, macrophages 15271960 50 malK BMEI1713 Macrophages 14979322 51 manB BMEII0899 Mice, macrophages, HeLa 14979322 52 mocC BMEII0570 Mice, HeLa 14979322 53 ndvB BMEI1837 Mice, HeLa 14979322 54 pgi BMEI1636...”
- Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model
Alcantara, Infection and immunity 2004 - “...BMEI1905 BMEII0606 BMEI2034 BMEII0428 BMEII0783 BMEI1123 BMEI0233 BMEI1848 BMEI0296 a Open reading frame designation in the B. melitensis genome. pected, the...”
PFL_5877 dihydroxy-acid dehydratase from Pseudomonas fluorescens Pf-5
Aligns to 17:607 / 613 (96.4%), covers 99.6% of TIGR00110, 837.1 bits
PVLB_01425 dihydroxy-acid dehydratase from Pseudomonas sp. VLB120
Aligns to 17:607 / 613 (96.4%), covers 99.6% of TIGR00110, 836.3 bits
Psyr_0469 Dihydroxy-acid dehydratase from Pseudomonas syringae pv. syringae B728a
Aligns to 17:607 / 615 (96.1%), covers 99.6% of TIGR00110, 835.6 bits
PA0353 dihydroxy-acid dehydratase from Pseudomonas aeruginosa PAO1
PA14_04630 dihydroxy-acid dehydratase from Pseudomonas aeruginosa UCBPP-PA14
Aligns to 17:607 / 612 (96.6%), covers 99.6% of TIGR00110, 835.4 bits
- Massively parallel mutant selection identifies genetic determinants of <i>Pseudomonas aeruginosa</i> colonization of <i>Drosophila melanogaster</i>
Miles, mSystems 2024 - “...Amino acids [E] + PA0036 trpB Amino acids [E] PA0649 trpG Amino acids [E] + PA0353 ilvD Amino acids [E] + PA4695 ilvH Amino acids [E] + PA4694 ilvC Amino acids [E] PA4696 ilvI Amino acids [E] PA5013 ilvE Amino acids [E] PA5204 argA Amino acids...”
- Reverse diauxie phenotype in Pseudomonas aeruginosa biofilm revealed by exometabolomics and label-free proteomics
Yung, NPJ biofilms and microbiomes 2019 - “...factor NR NR NR HemN PA1546 Oxygen-independent coproporphyrinogen-III oxidase NR NR NR NR NR IlvD PA0353 Dihydroxy-acid dehydratase NR NR NR NR NR KatA PA4236 Catalase NR o NR MtnB PA1683 Methylthioribulose-1-phosphate dehydratase NR NR NR NR NfuA PA1847 Fe/S biogenesis protein NR NR NR NR...”
- A systems biology approach to drug targets in Pseudomonas aeruginosa biofilm
Sigurdsson, PloS one 2012 - “...capsule UppS (PA3652) Biotin biosynthesis AccC (PA4848) Branched chain amino acid biosynthesis IlvC (PA4694), IlvD (PA0353) Cell envelope biosynthesis GlmU (PA5552), GlmS (PA5549), MurI (PA4662), MraY (PA4415), MurE (PA4417), MurC (PA4411), MurB (PA2977) DdlB (PA4410), DdlA (PA4201), MurA (PA4450),MurD (PA4414) Cell envelope biosynthesis- O-antigen RmlA (PA5163)...”
- Role of lon, an ATP-dependent protease homolog, in resistance of Pseudomonas aeruginosa to ciprofloxacin
Brazas, Antimicrobial agents and chemotherapy 2007 - “...73_D6 75_D3 81_D2 6_G1 80_B7 22_C5 53_F11 16_G12 PA0353 PA0836 PA1803 PA1803 PA1803 PA3082 PA3294 PA3617 PA3777 PA3965 PA4163 PA4316 PA4316 PA4422 PA4466 PA4700...”
- Genetics of Pseudomonas
Holloway, Bacteriological reviews 1969 - Pseudomonas aeruginosa core metabolism exerts a widespread growth-independent control on virulence
Panayidou, Scientific reports 2020 - “...( aroB ) 29 Valine, leucine and isoleucine biosynthesis (pau00290) PA14_62130 ( ilvC ) 30 PA14_04630 ( ilvD ) 31 PA14_62150 ( ilvH ) 32 PA14_62160 ( ilvI ) 33 PA14_23790 ( leuB ) 34 PA14_23750 ( leuC ) 35* Pyrimidine metabolism (pau00240) PA14_18710 ( pyrC...”
PFLU5797 dihydroxy-acid dehydratase from Pseudomonas fluorescens SBW25
Aligns to 17:607 / 613 (96.4%), covers 99.6% of TIGR00110, 833.1 bits
NMB1150 dihydroxy-acid dehydratase from Neisseria meningitidis MC58
Aligns to 17:614 / 619 (96.6%), covers 99.6% of TIGR00110, 832.3 bits
- Cationic antimicrobial peptide resistance in Neisseria meningitidis
Tzeng, Journal of bacteriology 2005 - “...in NMB1052 (dedA), and XZS17 with an insertion in NMB1150 (ilvD; dihydroxy acid dehydratase). All three of these genes are predicted (TopPred II [8] and PSORT...”
- “...mtrR-mtrC mtrD mtrD, NMB1052 mtrD, NMB0355 mtrD, NMB0355 mtrD, NMB1150 mtrD, lptA mtrD, lptA mtrD, lptA FIG. 2. Silver staining of whole-cell PK digestion of...”
PP_5128 dihydroxy-acid dehydratase from Pseudomonas putida KT2440
Aligns to 17:607 / 613 (96.4%), covers 99.6% of TIGR00110, 831.3 bits
- Functional characterization of the dbu locus for D-branched-chain amino acid catabolism in Pseudomonas putida
Fulton, Applied and environmental microbiology 2024 (secret) - A genome-scale metabolic reconstruction of Pseudomonas putida KT2440: iJN746 as a cell factory
Nogales, BMC systems biology 2008 - “...ilvA-2 SER_AL, THRD_L (+/-)* PP_4680 ilvB (ilvI) ACHBS, ACLS (-/-) PP_4678 ilvC KARA1, KARA2 (-/-) PP_5128 ilvD DHAD1, DHAD2 (-/-) PP_3511 ilvE VALTA, LEUTA, ILETA (-/-) PP_4679 ilvN(ilvH) ACHBS, ACLS (-/-) Leucine PP_1025 leuA IPPS (-/-) PP_1988 leuB IPMDr (-/-) PP_1985 leuC IPPMIa, IPPMIb (-/-) PP_1986...”
NP_879169 dihydroxy-acid dehydratase from Bordetella pertussis Tohama I
BP0289 dihydroxy-acid dehydratase from Bordetella pertussis Tohama I
Aligns to 17:614 / 619 (96.6%), covers 99.6% of TIGR00110, 829.5 bits
Q31QL1 Dihydroxy-acid dehydratase from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)
Synpcc7942_0626 dihydroxy-acid dehydratase from Synechococcus elongatus PCC 7942
Aligns to 17:612 / 619 (96.3%), covers 99.6% of TIGR00110, 829.4 bits
BB0431 dihydroxy-acid dehydratase from Bordetella bronchiseptica RB50
Aligns to 17:614 / 619 (96.6%), covers 99.6% of TIGR00110, 829.1 bits
Q5F8G6 Dihydroxy-acid dehydratase from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Aligns to 17:614 / 619 (96.6%), covers 99.6% of TIGR00110, 827.4 bits
- Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability
Diao, Frontiers in microbiology 2020 - “...Ethanolactive dehydrogenase/acetaldehydeactive reductase G 0 N 4.18 Q5F873 gltA Citrate synthase C 0 N 2.13 Q5F8G6 ilvD Dihydroxyacid dehydratase EG 0 N 1.73 Q5F9V2 NGO_0286 Uncharacterized protein S 0 N 1.75 A0A0M3GXE9 M736_02645 Conjugal transfer protein TrbB UW 0 N 2.36 A0A0M3GXY6 M736_02455 Phosphoribosyltransferase F 0...”
- “...and degradation (ngk00280) ( P < 0.05) ( Table 3 ). Six differentially expressed proteins (Q5F8G6, Q5F873, D6H9Y3, A0A1D3IB26, A0A1D3FF29, and D6HBH4) were involved in the three enrichment pathways, and all of the 6 proteins were downregulated. The function of Q5F873 (citrate synthase) was associated with...”
Tsac_0569 dihydroxy-acid dehydratase from Thermoanaerobacterium saccharolyticum JW/SL-YS485
Aligns to 14:553 / 554 (97.5%), covers 99.8% of TIGR00110, 826.8 bits
- Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress
Zhu, Scientific reports 2017 - “...5.177 8.6580.311 Tsac_0104 5-Nucleotidase domain-containing protein 1.84E-03 0.137 0.0960.015 ko00290: Valine, leucine and isoleucine biosynthesis Tsac_0569 Dihydroxy-acid dehydratase 1.56E-02 4.833 4.1730.527 Tsac_0564 Ketol-acid reductoisomerase 1.58E-02 4.817 4.2160.296 Tsac_0566 3-isopropylmalate dehydratase large subunit 1.89E-02 4.649 4.2660.252 Tsac_2182 Glu/Leu/Phe/Val dehydrogenase dimerization region 2.81E-02 0.009 7.5422.795 ko00620: Pyruvate metabolism...”
PF0942 dihydroxy-acid dehydratase from Pyrococcus furiosus DSM 3638
Aligns to 14:551 / 551 (97.6%), covers 99.8% of TIGR00110, 824.1 bits
XHV734_0458 dihydroxy-acid dehydratase from Xanthomonas hortorum pv. vitians
Aligns to 17:607 / 612 (96.6%), covers 99.6% of TIGR00110, 823.3 bits
WP_007877043 dihydroxy-acid dehydratase from Herbaspirillum sp. CF444
Aligns to 17:615 / 620 (96.6%), covers 99.6% of TIGR00110, 821.6 bits
Q9UZ03 Dihydroxy-acid dehydratase from Pyrococcus abyssi (strain GE5 / Orsay)
Aligns to 14:551 / 551 (97.6%), covers 99.8% of TIGR00110, 821.6 bits
Q2RTF9 Dihydroxy-acid dehydratase from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1)
Aligns to 17:612 / 617 (96.6%), covers 99.6% of TIGR00110, 820.3 bits
Rru_A1786 Dihydroxy-acid dehydratase from Rhodospirillum rubrum ATCC 11170
Aligns to 25:620 / 625 (95.4%), covers 99.6% of TIGR00110, 820.2 bits
PXO_03941 dihydroxy-acid dehydratase from Xanthomonas oryzae pv. oryzae PXO99A
Aligns to 17:607 / 612 (96.6%), covers 99.6% of TIGR00110, 820.2 bits
alr2771 dihydroxyacid dehydratase from Nostoc sp. PCC 7120
Aligns to 18:558 / 563 (96.1%), covers 99.8% of TIGR00110, 819.0 bits
- The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box
Oliveira, Life (Basel, Switzerland) 2015 - “...2 Transport and binding proteins - Alr2709 Alr2709 protein N 2 Conserved hypothetical protein - Alr2771 Dihydroxy-acid dehydratase NO 3 Amino acid biosynthesis Anabaena Alr2877 Bicarbonate transport bicarbonate-binding protein N 2 , NO 3 , NH 4 + Transport and binding proteins - Alr2887 Alr2887 protein...”
- “...acid biosynthesis and processing (All2533, Alr0237, Alr0880, Alr0996, Alr1381, All4287, Alr1742, Alr4853, All2315, Alr1004, Alr1313, Alr2771, Alr0608, Alr1080, Alr2328 Alr4907, All1683, All4464), sugar breakdown and processing (Alr3608, Alr4448, All3964, All0167, All0168, All0875, Alr0169, Alr2190, All4539) and phosphor scavenge and transport (All0207, All2843, All4575, Alr4238, Alr4976). Detection...”
Caur_2851 dihydroxy-acid dehydratase from Chloroflexus aurantiacus J-10-fl
Aligns to 18:558 / 561 (96.4%), covers 99.8% of TIGR00110, 815.1 bits
- Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus
Tang, BMC genomics 2011 - “...ilvA (Caur_2585, Caur_3892), isoleucine/leucine/valine biosynthesis (Caur_0041, Caur_0163 - 0169, Caur_0329 - 0331, Caur_0488, Caur_1435, and Caur_2851) Sulfur metabolism Sulfur reduction operon (Caur_0686 - 0692), a sulfate adenylyl-transferase/adenylylsulfate kinase (Caur_2113), cysK (Caur_1341), cysM (Caur_3489), sqr (Caur_3894, type II SQR), sulfotransferase (Caur_2114). B808-866 light-harvesting complex Caur_2090 (-subunit) and...”
ILVD_CAUVC / P55186 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see paper)
CC3044, CC_3044 dihydroxy-acid dehydratase from Caulobacter crescentus CB15
CCNA_03139 dihydroxy-acid dehydratase from Caulobacter crescentus NA1000
Aligns to 17:611 / 617 (96.4%), covers 99.6% of TIGR00110, 811.0 bits
- function: Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo- 3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3- dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.
catalytic activity: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O (RHEA:24809)
catalytic activity: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2- oxopentanoate + H2O (RHEA:27694)
cofactor: [2Fe-2S] cluster (Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.)
cofactor: Mg(2+)
subunit: Homodimer. - Global transcriptional response of Caulobacter crescentus to iron availability
da, BMC genomics 2013 - “...(CC3667), NADH ubiquinone oxidoreductase ( nuo genes CC1946, CC1944-43-42), glutamate synthase (CC3606) and dihydroxy-acid dehydratase (CC3044) (Table 4 ). In some cases, at least part of the operons ( nuo and CC3607) was downregulated by both iron limitation and fur mutation (Figure 2 B). A possible...”
- “...CCNA_02223 Beta-lactamase, type II (Zinc) 2.83 CC_2140 CCNA_02224 Methylenetetrahydrofolate reductase 2.39 CC_2840 CCNA_02933 Aminopeptidase 2.14 CC_3044 CCNA_03139 Dihydroxy-acid dehydratase (Fe-S cluster) 3.35 CC_3246 CCNA_03355 Acylamino-acid-releasing enzyme 2.20 CC_3606 CCNA_03721 Glutamate synthase (NADPH) small chain 2.30 CC_3607 b CCNA_03722 Glutamate synthase (NADPH) large chain (Fe-S cluster) 2.51...”
- Global transcriptional response of Caulobacter crescentus to iron availability
da, BMC genomics 2013 - “...Beta-lactamase, type II (Zinc) 2.83 CC_2140 CCNA_02224 Methylenetetrahydrofolate reductase 2.39 CC_2840 CCNA_02933 Aminopeptidase 2.14 CC_3044 CCNA_03139 Dihydroxy-acid dehydratase (Fe-S cluster) 3.35 CC_3246 CCNA_03355 Acylamino-acid-releasing enzyme 2.20 CC_3606 CCNA_03721 Glutamate synthase (NADPH) small chain 2.30 CC_3607 b CCNA_03722 Glutamate synthase (NADPH) large chain (Fe-S cluster) 2.51 Chemotaxis...”
FNP_0059 dihydroxy-acid dehydratase from Fusobacterium polymorphum ATCC 10953
Aligns to 15:553 / 553 (97.5%), covers 99.6% of TIGR00110, 808.0 bits
- Genome sequence of Fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium
Karpathy, PloS one 2007 - “...of these amino acids. The predicted products encoded by this locus include dihydroxy-acid dehydratase (IlvD, FNP_0059), threonine ammonia-lyase (IlvA, FNP_0060), acetolactate synthase (IlvB and IlvN, FNP_0061 and FNP_0062), 2-isopropyl malate synthase (LeuA, FNP_0063), 3-isopropyl malate synthase (LeuC and LeuD, FNP_0064 and FNP_0065), isopropyl malate dehydrogenase (LeuB,...”
ZMO1792 dihydroxy-acid dehydratase from Zymomonas mobilis subsp. mobilis ZM4
Aligns to 17:612 / 618 (96.4%), covers 99.6% of TIGR00110, 800.8 bits
- Systematic investigation of TetR-family transcriptional regulators and their roles on lignocellulosic inhibitor acetate tolerance in Zymomonas mobilis
Xiao, Frontiers in bioengineering and biotechnology 2024 - “...after ZMO0281 knockout may be also related to the repression of sucrose hydrolysis. In addition, ZMO1792 ( ilvD ) which related to amino acid metabolism was found to be repressed as well ( Supplementary Table S3 ). However, about 20 of the downregulated genes by ZM...”
- A reconciliation of genome-scale metabolic network model of Zymomonas mobilis ZM4
Nouri, Scientific reports 2020 - “...genes/inconsistent Up-regulated metabolic genes/inconsistent ZMO0172 Thiamine biosynthesis protein ZMO1853 Dihydrodipicolinate synthase ZMO0889 Aldose 1-epimerase precursor ZMO1792 Dihydroxy-acid dehydratase ZMO0239 ATP synthase alpha subunit ZMO1887 Isochorismatase ZMO0241 ATP synthase beta subunit ZMO1879 Delta-aminolevulinic acid dehydratase ZMO0367 Glucose-6-phosphate dehydrogenase ZMO1489 3-deoxy-D-manno-octulosonate cytidylyltransferase ZMO0465 Triosephosphate isomerase ZMO1496 Phosphoenolpyruvate carboxylase...”
- Metabolic engineering of Zymomonas mobilis for anaerobic isobutanol production
Qiu, Biotechnology for biofuels 2020 - “...Based on gene homology search results, Z. mobilis possesses four genes ZMO01139, ZMO1140, ZMO1141, and ZMO1792 encoding the enzymes Als, IlvC, and IlvD, respectively. ZMO01139 and ZMO1140 encode the large and small (regulatory) subunits of Als, respectively. These enzymes are the first three enzymes in the...”
LBYS11_13595 dihydroxy-acid dehydratase from Lysinibacillus sp. YS11
Aligns to 14:556 / 556 (97.7%), covers 99.8% of TIGR00110, 800.1 bits
CD2014 dihydroxy-acid dehydratase from Clostridium difficile 630
Aligns to 13:551 / 551 (97.8%), covers 99.8% of TIGR00110, 798.1 bits
SE1654 dihydroxy-acid dehydratase from Staphylococcus epidermidis ATCC 12228
Aligns to 14:557 / 562 (96.8%), covers 99.8% of TIGR00110, 793.9 bits
Cp258_0893 dihydroxy-acid dehydratase from Corynebacterium pseudotuberculosis 258
Aligns to 17:612 / 613 (97.2%), covers 99.6% of TIGR00110, 793.8 bits
CAC3170 Dihydroxy-acid dehydratase from Clostridium acetobutylicum ATCC 824
CA_C3170 dihydroxy-acid dehydratase from Clostridium acetobutylicum ATCC 824
Aligns to 14:549 / 552 (97.1%), covers 99.6% of TIGR00110, 792.7 bits
- Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum
Amador-Noguez, Applied and environmental microbiology 2011 - “...biosynthesis, cac3173, cac3172, cac3169, cac3176, cac0091, cac3170, cac1479, cac0273, cac3174, and cac3171; serine biosynthesis, cac0014, cac0015, and cac0263;...”
- Meta-analysis and functional validation of nutritional requirements of solventogenic Clostridia growing under butanol stress conditions and coutilization of D-glucose and D-xylose
Heluane, Applied and environmental microbiology 2011 - “...CAC2389 CAC2390 CAC2634 CAC2708 CAC2711 CAC2712 CAC3164 CAC3170 CAC3348 CAC3462 CAC3596 CAC3680 CAC3681 0.40506 1.51803 1.07553 1.7949 2.70695 0.54094 0.61838...”
- High-quality-draft genome sequence of the fermenting bacterium Anaerobium acetethylicum type strain GluBS11T (DSM 29698)
Patil, Standards in genomic sciences 2017 - “...was also reported for the gluconate-fermenting C. acetobutylicum ATCC 824 T , where the gene CA_C3170 was predicted to encode a 6-phosphogluconate dehydratase and BlastP analysis indicated that it is a dihydroxy acid dehydratase primarily involved in the synthesis of amino acids [ 47 , 48...”
- “...and Anaerostipes caccae DSM 14662 T , respectively, and showed only 40-60% identity with gene CA_C3170 of C. acetobutylicum ATCC 824 T . Therefore, genes Ga0116910_10068 and Ga0116910_101679 most likely encode a dihydroxy acid dehydratase that is involved in amino acid synthesis rather than in KDPG...”
- Fermentation of oxidized hexose derivatives by Clostridium acetobutylicum
Servinsky, Microbial cell factories 2014 - “...unlikely that gluconokinase is present in the organism [ 28 ]. MetaCyc also predicted that CA_C3170 encodes 6-phosphogluconate dehydratase, however the genomic context of the gene is inconsistent with that function. A BlastP analysis of the CA_C3170 translated amino acid sequence indicated it is a dihydroxyacid...”
DIP1096 dihydroxy-acid dehydratase from Corynebacterium diphtheriae NCTC 13129
Aligns to 17:612 / 613 (97.2%), covers 99.6% of TIGR00110, 791.2 bits
- Corynebacterium diphtheriae Proteome Adaptation to Cell Culture Medium and Serum
Möller, Proteomes 2021 - “...expression level in serum compared to BHI and RPMI 1640. Cluster four comprises eight proteins (DIP1096, DIP0409, DIP0745, DIP0009, DIP0154, DIP0948, DIP0833, and DIP1131). Significantly increased abundance of proteins from bacteria grown in BHI medium are represented in cluster five. This cluster includes 15 proteins (DIP1636,...”
BU600 dihydroxy-acid dehydratase from Buchnera aphidicola str. APS (Acyrthosiphon pisum)
Aligns to 17:611 / 617 (96.4%), covers 99.6% of TIGR00110, 788.8 bits
- A substrate ambiguous enzyme facilitates genome reduction in an intracellular symbiont
Price, BMC biology 2014 - “...ilvM x EG10501 2.2.1.6 acetolactate synthase (ALS) ilvC BU599 EG10495 1.1.1.86 ketol-acid reductoisomerase (KARI) ilvD BU600 EG10496 4.2.1.9 dihydroxyacid dehydratase (DHAD) panB BU197 EG11675 2.1.2.11 ketopantoate hydroxymethltransferase (KPHMT) panE x EG13271 1.1.1.169 ketopantoate reductase (KPR) panD x EG11747 4.1.1.11 L-aspartate--decarboxylase (ADC) panC BU196 EG11746 6.3.2.1 pantothenate...”
CIBE_4586 dihydroxy-acid dehydratase from Clostridium beijerinckii
Aligns to 14:552 / 552 (97.6%), covers 99.6% of TIGR00110, 788.7 bits
Q64PS6 Dihydroxy-acid dehydratase from Bacteroides fragilis (strain YCH46)
Aligns to 18:599 / 600 (97.0%), covers 99.4% of TIGR00110, 788.6 bits
Q8A608 Dihydroxy-acid dehydratase from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
Aligns to 18:599 / 600 (97.0%), covers 99.4% of TIGR00110, 786.6 bits
F502_03482 dihydroxy-acid dehydratase from Clostridium pasteurianum DSM 525 = ATCC 6013
Aligns to 14:549 / 549 (97.6%), covers 99.6% of TIGR00110, 786.2 bits
Q5L9I8 Dihydroxy-acid dehydratase from Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / LMG 10263 / NCTC 9343 / Onslow / VPI 2553 / EN-2)
Aligns to 18:599 / 600 (97.0%), covers 99.4% of TIGR00110, 785.8 bits
PMM0774 Dihydroxy-acid dehydratase from Prochlorococcus marinus sp. MED4
Aligns to 17:557 / 559 (96.8%), covers 99.8% of TIGR00110, 784.5 bits
- Dynamic Allocation of Carbon Storage and Nutrient-Dependent Exudation in a Revised Genome-Scale Model of Prochlorococcus
Ofaim, Frontiers in genetics 2021 - “...( Altschul et al., 1990 ) from which we identified 6PG-dehydratase (EC: 4.2.1.12) encoded by PMM0774, thereby completing this pathway in the model reconstruction. (iv) The revised model was checked for redox and elemental balance. Since the biomass function was based on experimental data ( Casey...”
- “...Supplementary Material 1 Results from the BLAST-search used to identify 6PG-dehydratase (EC: 4.2.1.12) encoded by PMM0774 in P. marinus MED4. Click here for additional data file. Supplementary Material 2 Memote snapshot report of i SO595. Click here for additional data file. References Aharonovich D. Sher D....”
SAUSA300_RS11035, USA300HOU_RS11060 dihydroxy-acid dehydratase from Staphylococcus aureus subsp. aureus USA300_TCH1516
SAUSA300_2006 dihydroxy-acid dehydratase from Staphylococcus aureus subsp. aureus USA300_FPR3757
Aligns to 14:557 / 562 (96.8%), covers 99.8% of TIGR00110, 784.3 bits
- Metabolic diversity of human macrophages: potential influence on Staphylococcus aureus intracellular survival
Bertrand, Infection and immunity 2024 - “...). Among the top 10 essential genes that were unique to IL-10-treated HMDMs were ilvD (SAUSA300_RS11035), gudB (SAUSA300_RS04645), hemX (SAUSA300_RS08820), miaA (SAUSA300_RS06455), and ndh2 (SAUSA300_RS04560) ( Fig. 3A ; Table 1 ). In terms of untreated HMDMs, unique genes included itaA (SAUSA300_RS04930), pckA (SAUSA300_RS09470), katA (SAUSA300_RS06680),...”
- Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA
Xu, Frontiers in microbiology 2016 - “...protein S17 USA300HOU_RS04900 oppD1 1.91 4.92E-03 Oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein USA300HOU_RS11060 ilvD 1.89 1.79E-02 Dihydroxy-acid dehydratase USA300HOU_RS07110 dapB 1.84 1.91E-02 Dihydrodipicolinate reductase USA300HOU_RS13735 1.82 1.46E-02 Transcriptional regulator USA300HOU_RS08760 rplU 1.79 1.10E-02 Ribosomal protein L21 USA300HOU_RS07115 dapD 1.75 2.17E-02 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase USA300HOU_RS07100...”
- Cigarette Smoke Extract-Exposed Methicillin-Resistant Staphylococcus aureus Regulates Leukocyte Function for Pulmonary Persistence
Kulkarni, American journal of respiratory cell and molecular biology 2016 - “...SAUSA300_2521 SAUSA300_0204 SAUSA300_0083 SAUSA300_2008 SAUSA300_2006 leuD SAUSA300_0084 SAUSA300_0310 SAUSA300_0438 SAUSA300_0107 SAUSA300_1726 SAUSA300_0846...”
- The DUF59 Containing Protein SufT Is Involved in the Maturation of Iron-Sulfur (FeS) Proteins during Conditions of High FeS Cofactor Demand in Staphylococcus aureus
Mashruwala, PLoS genetics 2016 - “...] NE892 SAUSA300_2012( leuC ):: TN ( ermB ) LAC NARSA [ 105 ] NE718 SAUSA300_2006( ilvD ):: TN ( ermB ) LAC NARSA [ 105 ] JMB1432 fur :: tet LAC [ 106 ] JMB1163 acnA :: tet LAC [ 107 ] JMB3537 acnA ::...”
- Differential gene expression in Staphylococcus aureus exposed to Orange II and Sudan III azo dyes
Pan, Journal of industrial microbiology & biotechnology 2015 - “...SAUSA300_1231 SAUSA300_0864 SAUSA300_2538 SAUSA300_1808 SAUSA300_1916 SAUSA300_0914 SAUSA300_2006 ilvD EG Amino acid permease family protein Amino acid ABC...”
- “...cytoplasmic argininosuccinate synthase and ilvD (SAUSA300_2006) encoding dihydroxy-acid dehydratase were observed to be significantly up-regulated (3.14...”
- Bacillithiol has a role in Fe-S cluster biogenesis in Staphylococcus aureus
Rosario-Cruz, Molecular microbiology 2015 - “...NE892 SAUSA300_2013( leuD )::Tn( ermB ) JE2 ( Fey et al. , 2013 ) NE718 SAUSA300_2006( ilvD )::Tn( ermB ) JE2 ( Fey et al. , 2013 ) NE861 SAUSA300_1246( acnA )::Tn( ermB ) JE2 ( Fey et al. , 2013 ) NE1932 SASUA300_1513( sodA )::Tn(...”
- “...( ermB ) LAC JMB5272 SAUSA300_1349 ::kanR , 2013( leuD )::Tn( ermB ) LAC JMB3804 SAUSA300_2006( ilvD )::Tn( ermB ) LAC JMB4417 SAUSA300_1071( bshC ) , 2006( ilvD )::Tn( ermB ) LAC JMB3702 SAUSA300_1246( acnA )::Tn( ermB ) LAC JMB5225 SAUSA300_1349( bshA ) ::kanR , 1246(...”
- Nfu facilitates the maturation of iron-sulfur proteins and participates in virulence in Staphylococcus aureus
Mashruwala, Molecular microbiology 2015 - “...SAUSA300_0380( ahpC ):: TN ( ermB ) NARSA( Fey et al. , 2013 ) NE718 SAUSA300_2006 (ilvD)::TN(ermB) NARSA( Fey et al. , 2013 ) NE1175 SAUSA300_0839( nfu ):: TN ( ermB ) NARSA( Fey et al. , 2013 ) NE1932 sodA::TN(ermB) (SAUSA300_1513) NARSA( Fey et al....”
- “...al. , 2002 ) pEPSA5_ nfu SAUSA300_0839 pEPSA5_ acnA SAUSA300_1246 pEPSA5_FLAG_ leuCD SAUSA300_2012-3 pEPSA5_ IlvD SAUSA300_2006 pEPSA5_ ahpCF SAUSA300_0379-80 pCM11 Transcriptional fusion containing promoterless gfp ( Malone et al. , 2009 ) pCM11_ acnA p SAUSA300_1246 promoter pCM11_ dps p pCM11_ recA p SAUSA300_1178 promoter pET20b...”
Q8NQZ9 dihydroxy-acid dehydratase (EC 4.2.1.9) from Corynebacterium glutamicum (see 2 papers)
NCgl1219 dihydroxy-acid dehydratase from Corynebacterium glutamicum ATCC 13032
cg1432 dihydroxy-acid dehydratase from Corynebacterium glutamicum ATCC 13032
Aligns to 17:612 / 613 (97.2%), covers 99.6% of TIGR00110, 784.3 bits
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics
Zhang, Scientific reports 2018 - “...0.00 1295.30 2.6 25 NCgl0368 cg0454 TetR family transcriptional regulator 5.41/24817.09 0.00 571.02 NC 32 NCgl1219 cg1432 ilvD Dihydroxy-acid dehydratase 5.18/64674.11 4354.80 1888.03 2.8 33 NCgl1219 cg1432 ilvD Dihydroxy-acid dehydratase 5.18/64674.11 0.00 667.12 2.8 34 NCgl2930 cg3362 trpCF Indole-3-glycerol phosphate synthase 5.15/50477.16 1290.96 0.00 3.4 38...”
- High-throughput screening of a Corynebacterium glutamicum mutant library on genomic and metabolic level
Reimer, PloS one 2014 - “...NCgl1536 encoding the ribulose-phosphate 3-epimerase, EC 5.1.3.1) or the valine, leucine and isoleucine pathway ( NCgl1219 encoding the dihydroxy-acid dehydratase, EC 4.2.1.9 and NCgl0245 encoding the 2-isopropylmalate synthase, EC 2.3.3.13). Also an intergenic mutation (mutant P21E12), that did not hit a gene directly was found within...”
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics
Zhang, Scientific reports 2018 - “...1295.30 2.6 25 NCgl0368 cg0454 TetR family transcriptional regulator 5.41/24817.09 0.00 571.02 NC 32 NCgl1219 cg1432 ilvD Dihydroxy-acid dehydratase 5.18/64674.11 4354.80 1888.03 2.8 33 NCgl1219 cg1432 ilvD Dihydroxy-acid dehydratase 5.18/64674.11 0.00 667.12 2.8 34 NCgl2930 cg3362 trpCF Indole-3-glycerol phosphate synthase 5.15/50477.16 1290.96 0.00 3.4 38 NCgl1023...”
- Ciprofloxacin triggered glutamate production by Corynebacterium glutamicum
Lubitz, BMC microbiology 2016 - “...of glutamate production by C. glutamicum . The mechanosensitive channel protein MscS encoded by yggB (cg1432) is involved in the export of glutamate and in its absence glutamate production is reduced about four to five fold [ 26 ]. To test whether YggB is important for...”
- Exploring the role of sigma factor gene expression on production by Corynebacterium glutamicum: sigma factor H and FMN as example
Taniguchi, Frontiers in microbiology 2015 - “...trmU tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase 1.5 1.7 6.6E-4 4.8E-3 cg1398 Conserved hypothetical protein 1.7 2.4 1.9E-2 2.3E-2 cg1432 ilvD Dihydroxy-acid dehydratase 1.9 1.9 2.0E-4 6.7E-4 cg1628 Putative hydrolase, alpha/beta superfamily 2.5 1.9 4.8E-2 9.1E-3 cg1671 Putative membrane-associated GTPase 1.7 1.3 3.9E-2 1.4E-2 cg1687 Putative transcriptional regulatory protein 1.4...”
- “...( http://www.uniprot.org/ ) identified putative iron sulfur cluster-containing proteins encoded by cg0616 ( fdhD ), cg1432 ( ilvD ), cg2206 ( ispG ) and cg2423 ( lipA ), proteins predicted to contain NAD(P)H binding sites encoded by cg0184, cg0616 ( fdhD ), cg1778 ( zwf ),...”
- Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum
Busche, BMC genomics 2012 - “...Conserved hypothetical protein 6.19 cg0617* Hypothetical protein 4.20 cg1288 Putative multidrug efflux permease, MFS-type 3.94 cg1432 ilvD Dihydroxy-acid dehydratase 3.84 cg1398 Conserved hypothetical protein 3.78 cg0614 Hypothetical protein 3.71 cg0616 fdhD Putative formate dehydrogenase, FdhD-family 3.71 cg1397* trmU tRNA (5-methylaminomethyl-2-thiouridylate) -methyltransferase 3.71 cg2423 lipA Lipoyl synthetase...”
SAV2053 dihydroxy-acid dehydratase from Staphylococcus aureus subsp. aureus Mu50
SA1858 dihydroxy-acid dehydratase from Staphylococcus aureus subsp. aureus N315
SAOUHSC_02281 dihydroxy-acid dehydratase from Staphylococcus aureus subsp. aureus NCTC 8325
Aligns to 14:557 / 562 (96.8%), covers 99.8% of TIGR00110, 784.1 bits
- Two novel point mutations in clinical Staphylococcus aureus reduce linezolid susceptibility and switch on the stringent response to promote persistent infection
Gao, PLoS pathogens 2010 - “...protease HtrA 3.34 SAV1083 argJ bifunctional ornithine acetyltransferase/N-acetylglutamate synthase, ArgJ 2.61 SAV1085 ornithine aminotransferase 2.69 SAV2053 ilvD dihydroxy-acid dehydratase 6.03 SAV2054 ilvB acetolactate synthase large subunit 4.54 SAV2057 leuA 2-isopropylmalate synthase 7.07 SAV2058 leuB 3-isopropylmalate dehydrogenase 2.58 SAV2059 leuC isopropylmalate isomerase large subunit 4.81 SAV2060 leuD...”
- Determination of essentiality and regulatory function of staphylococcal YeaZ in branched-chain amino acid biosynthesis
Lei, Virulence 2015 - “...YeaZ a ORF (N315) Gene Fold Change Sa0512 Sa1098 Sa1557 Sa1858 Sa1862 Sa1866 ilvE codY ccpA ilvD leuA ilvA 0.97 0.64 1.16 17.45 133.9 289.01 Note: aThe fold...”
- The essentiality of staphylococcal Gcp is independent of its repression of branched-chain amino acids biosynthesis
Lei, PloS one 2012 - “...ORF (N315) Gene Fold Change a Sa0512 ilvE 0.97 Sa1098 codY 0.78 Sa1557 ccpA 3.24 Sa1858 ilvD 5.45 Sa1862 leuA 68.59 Sa1866 ilvA 111.37 a The fold change represents the transcription levels of genes with the depletion of Gcp compared with those during the induction of...”
- Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism
Bohn, Nucleic acids research 2010 - “...) 0.37 0.48 14.80 Methylcitrate synthase SA1553 ( fhs ) 0.30 f 14.08 Formatetetrahydrofolate ligase SA1858 ( ilvD ) 3.45 1.90 Dihydroxy-acid dehydratase SA1859 ( ilvB ) 2.75 Acetolactate synthase large subunit SA1860 ( ilvH ) 3.12 Acetolactate synthase 1 regulatory subunit SA1861 ( ilvC )...”
- “...and total RNA were extracted. a SA0845 to SA849, SA1088-SA1089, SA1367-SA1365, SA1435 to SA1432, SA1518-SA1517, SA1858 to SA1865, likely organized in six distinct operons, respectively (genes for the same operon are expected to have a RsaE-dependent coordinated regulation). b Gene expression ratios between strains containing pAT12-RsaE...”
- Transcriptional signature following inhibition of early-stage cell wall biosynthesis in Staphylococcus aureus
O'Neill, Antimicrobial agents and chemotherapy 2009 - “...SA1254 SA1255 SA1256 SA1257 SA1532 SA1545 SA1546 SA1691 SA1858 SA1859 SA1860 SA1861 SA1862 SA1863 SA1864 SA1865 SA1866 SA2112 SA2221 SA2235 SA2236 SA2237 SA2240...”
- Microarray analysis of toxicogenomic effects of ortho-phenylphenol in Staphylococcus aureus
Jang, BMC genomics 2008 - “...SA1211 0.000158 -2.3 ATP-binding ABC transporter protein opp -2F Amino acid transport and metabolism sa_c4209s3561_a_at SA1858 0.000144 -6.2 dihydroxy-acid dehydratase (DAD) ilv D Amino acid transport and metabolism, Coenzyme metabolism sa_c4213s3565_a_at SA1859 0.000431 -8.0 acetolactate synthase isozyme III large subunit (AHAS-III) ilv B Amino acid transport...”
- “..."amino acid transport and metabolism", which contained half of the genes in that group. Further, SA1858 ( ilv D)-SA1859 ( ilv B) and SA1861 ( ilv C)-SA1862 ( leu A)-SA1863 ( leu B)-SA1864 ( leu C)-SA1865 ( leu D)-SA1866 ( ilv A) which were downregulated (table...”
- The roles of cell wall inhibition responsive protein CwrA in the pathogenicity of <i>Staphylococcus aureus</i>
Han, Virulence 2024 - “...SAOUHSC_00899 argG Argininosuccinate synthase; ArgG 6.8 SAOUHSC_00898 argH Argininosuccinate lyase; ArgH 6.3 2-Oxocarboxylic acid metabolism SAOUHSC_02281 Dihydroxy-acid dehydratase 1.7 Nitrogen metabolism SAOUHSC_02671 narK Putative nitrate transporter; NarK 1.9 SAOUHSC_02679 narJ Probable nitrate reductase molybdenum cofactor assembly chaperone; NarJ 1.2 SAOUHSC_02684 nasD Assimilatory nitrite reductase; NasD 1.1...”
- An extensively validated whole-cell biosensor for specific, sensitive and high-throughput detection of antibacterial inhibitors targeting cell-wall biosynthesis
Galarion, The Journal of antimicrobial chemotherapy 2023 - “...former case as candidates for generating CWBI-responsive biosensors: gltB (SAOUHSC_00435), oppB (SAOUHSC_00923), sgtB (SAOUHSC_02012), ilvD (SAOUHSC_02281) and ORF2768 (SAOUHSC_03021). To our knowledge, only one of these ( sgtB ) has previously been employed as the basis for a staphylococcal CWBI biosensor. 9 For each of these,...”
- Antibacterial Components and Modes of the Methanol-Phase Extract from Commelina communis Linn
Liu, Plants (Basel, Switzerland) 2023 - “...also significantly downregulated (0.132-fold to 0.331-fold) ( p < 0.05), including a dihydroxy-acid dehydratase ( SAOUHSC_02281 ), an acetolactate synthase large subunit biosynthetic type ( SAOUHSC_02282 ), a conserved hypothetical protein ( SAOUHSC_02283 ), a ketol-acid reductoisomerase ( SAOUHSC_02284 ), a 2-isopropylmalate synthase ( SAOUHSC_02285 ),...”
- “...Ketol-acid reductoisomerase SAOUHSC_02283 0.184 Conserved hypothetical protein SAOUHSC_02282 0.208 Acetolactate synthase large subunit biosynthetic type SAOUHSC_02281 0.331 Dihydroxy-acid dehydratase ABC transporters SAOUHSC_00634 0.012 ABC transporter substrate-binding protein putative SAOUHSC_00636 0.014 Iron (chelated) ABC transporter permease protein putative SAOUHSC_00637 0.015 Conserved hypothetical protein SAOUHSC_01945 0.095 Membrane protein...”
- Teg58, a small regulatory RNA, is involved in regulating arginine biosynthesis and biofilm formation in Staphylococcus aureus
Manna, Scientific reports 2022 - “...3.41 SAOUHSC_02564 Urease accessory protein, ureG 4811 1747 2.75 Valine, leucine, and isoleucine biosynthesis pathway SAOUHSC_02281 Dihydroxy-acid dehydratase, ilvD 147 501 3.41 SAOUHSC_02282 Acetolactate synthase large subunit, ilvB 246 804 3.27 SAOUHSC_02284 2-dehydropantoate 2-reductase, ilvC 315 906 2.88 SAOUHSC_02283 Acetolactate synthase small subunit, ilvH 283 671...”
- The Staphylococcus aureus KdpDE two-component system couples extracellular K+ sensing and Agr signaling to infection programming
Xue, Infection and immunity 2011 - “...SAOUHSC_01389 SAOUHSC_00113 SAOUHSC_01619 SAOUHSC_01825 SAOUHSC_02281 SAOUHSC_02282 SAOUHSC_02283 SAOUHSC_02671 SAOUHSC_02679 SAOUHSC_02680 SAOUHSC_02682...”
SAV_4716 dihydroxy-acid dehydratase from Streptomyces avermitilis MA-4680
Aligns to 17:613 / 617 (96.8%), covers 99.6% of TIGR00110, 783.5 bits
- SAV742, a Novel AraC-Family Regulator from Streptomyces avermitilis, Controls Avermectin Biosynthesis, Cell Growth and Development
Sun, Scientific reports 2016 - “...the large subunit of acetolactate synthase), ilvC (sav_2731 , encoding a putative ketol-acid reductoisomerase), ilvD1 (sav_4716 , encoding a putative dihydroxy-acid dehydratase) and ilvE (sav_2717 , encoding a putative branched-chain amino acid aminotransferase); in oxidative phosphorylation: ndh1 (sav_1892 , encoding a putative NADH dehydrogenase) and ctaD2...”
C4ZAW6 Dihydroxy-acid dehydratase from Agathobacter rectalis (strain ATCC 33656 / DSM 3377 / JCM 17463 / KCTC 5835 / VPI 0990)
Aligns to 14:553 / 559 (96.6%), covers 99.6% of TIGR00110, 782.9 bits
SXYL_00875 dihydroxy-acid dehydratase from Staphylococcus xylosus
Aligns to 14:557 / 562 (96.8%), covers 99.8% of TIGR00110, 782.7 bits
Dde_0116 dihydroxy-acid dehydratase from Desulfovibrio desulfuricans G20
Aligns to 14:555 / 555 (97.7%), covers 99.8% of TIGR00110, 781.4 bits
Ssal_00131 dihydroxy-acid dehydratase from Streptococcus salivarius 57.I
Aligns to 14:569 / 573 (97.0%), covers 99.3% of TIGR00110, 780.1 bits
MSMEG_1290 dihydroxy-acid dehydratase from Mycobacterium smegmatis str. MC2 155
Aligns to 21:615 / 622 (95.7%), covers 99.6% of TIGR00110, 779.8 bits
NWMN_1960 dihydroxy-acid dehydratase from Staphylococcus aureus subsp. aureus str. Newman
SACOL2042 dihydroxy-acid dehydratase from Staphylococcus aureus subsp. aureus COL
Aligns to 14:557 / 562 (96.8%), covers 99.8% of TIGR00110, 779.7 bits
- (p)ppGpp/GTP and Malonyl-CoA Modulate Staphylococcus aureus Adaptation to FASII Antibiotics and Provide a Basis for Synergistic Bi-Therapy
Pathania, mBio 2021 - “...triggers (p)ppGpp synthesis ( 24 ) ( Fig.1A and data not shown). P ilvD -lacZ (NWMN_1960) and P oppB -lacZ (NWMN_0856) were upregulated, and P cshA -lacZ (NWMN_1985) was downregulated by mupirocin. Nutrient starvation during stationary phase induces the stringent response in E. coli ( 16...”
- “...used to transform DNA in S. aureus ( 45 ). (p)ppGpp sensors. The genes ilvD (NWMN_1960) and oppB (NWMN_0856) are upregulated, while the cshA (NWMN_1985) gene is downregulated, upon stringent response induction ( 24 , 40 , 46 ). The corresponding stringent response sensors P ilvD...”
- CodY in Staphylococcus aureus: a regulatory link between metabolism and virulence gene expression
Pohl, Journal of bacteriology 2009 - “...NWMN_1348 NWMN_1616 NWMN_1617 NWMN_1749 NWMN_1750 NWMN_1960 NWMN_1961 NWMN_1962 NWMN_1963 NWMN_1964 NWMN_1965 NWMN_1966 NWMN_2347 NWMN_2500 NWMN_2501 NWMN_2571...”
- In vitro and in vivo models of Staphylococcus aureus endophthalmitis implicate specific nutrients in ocular infection
Sadaka, PloS one 2014 - “...5.0 (1.9) 5.6 (1.2) ig_SACOL2041-2 Intergenic region upstream ofSACOL2042 ilvD ( ilvD promoterregion) 23.4 (4.3) SACOL2042 ilvD Dihydroxy-acid dehydratase 48.5 (1.4) 11.3 (5.4) 26.4 (1.4) SACOL2043 ilvB Acetolactate synthase large subunit 80.2 (1.2) 39.4 (1.2) SACOL2044 Acetolactate synthase 1 regulatory subunit 157.6 (1.7) 8.6 (3.1) 85.9...”
- Global analysis of the Staphylococcus aureus response to mupirocin
Reiss, Antimicrobial agents and chemotherapy 2012 - “...typA SACOL1136 SACOL1206 ileS SACOL1272 codY SACOL1759 SACOL2042 ilvD SACOL2054 sigB SACOL2058 SACOL2131 SACOL2379 SACOL2484 csb7 SACOL2511 fnbA SACOL2597 a b...”
- “...Asp23 IleS SACOL2050 SACOL2043 IlvA2 IlvB SACOL2045 IlvC SACOL2042 IlvD SACOL2049 LeuD Asp23-4 IleS-2 IleS-3 IlvA2-1 IlvB-1 IlvB-2 IlvC-1 IlvC-3 IlvD-1 IlvD-2...”
- A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus
Chen, Molecular microbiology 2009 - “...5.2 Fructose permiase SACOL2525 gntK 6.5 + Gluconokinase SACOL2516 gntR 5.6 + Gluconate operon repressor SACOL2042 ilvD 3.2 -2.3 Dihydroxy-acid dehydrates SACOL2183 lacD 2.0 Tagatose 1, 6-diphosphate aldrase SACOL0248 lrgB 3.2 Holinlike protein SACOL1428 lysC 4.4 Aspartokinase, and subunits SACOL1551 malA 4.7 -glucosidase SACOL0192 msmX 2.1...”
CTN_RS00550 dihydroxy-acid dehydratase from Thermotoga neapolitana DSM 4359
Aligns to 14:553 / 554 (97.5%), covers 99.6% of TIGR00110, 777.9 bits
- CO2-Induced Transcriptional Reorganization: Molecular Basis of Capnophillic Lactic Fermentation in Thermotoga neapolitana
d'Ippolito, Frontiers in microbiology 2020 - “...). This evidence was further validated by RT-PCR that showed up-regulation of 6-phosphogluconate dehydratase (EDD CTN_RS00550) that control the key step of ED and down-regulation of 6-phosphofructokinase (PFK CTN_RS02345) that is the regulator enzyme of EMP ( Supplementary Figure 2 ). Analysis of the genes of...”
- “...Pyruvate kinase OPP and EntnerDoudoroff common enzymes CTN_RS07110 4.84 Glucose-6-phosphate 1-dehydrogenase CTN_RS07115 5.50 6-Phosphogluconolactonase EntnerDoudoroff CTN_RS00550 5.83 6-Phosphogluconate dehydratase a CTN_RS03140 1.26 2-Dehydro-3-deoxyphosphogluconate aldolase OPP CTN_RS01150 1.40 6-Phosphogluconate dehydrogenase. Decarboxylating CTN_RS04470 1.64 Ribulose-phosphate-epimerase CTN_RS07445 NDE Ribose-5-phosphate isomerase CTN_RS04700 1.81 Transketolase CTN_RS08090 1.60 Transketolase. C-terminal subunit CTN_RS08085...”
Q8F219 Dihydroxy-acid dehydratase from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Aligns to 20:559 / 560 (96.4%), covers 99.8% of TIGR00110, 775.9 bits
- The Aspergillus fumigatus dihydroxyacid dehydratase Ilv3A/IlvC is required for full virulence
Oliver, PloS one 2012 - “...(NP_883684.1) Bordetella parapertussis ; Cagg1 (AOH553_9CHLR) Chloroflexus aggregans ; Cvib1 (Q9A8D3) Caulobacter vibroides ; Lint1 (Q8F219) Leptospira interrogans ; Meth1 (ZP_01850751.1) Methylobacterium sp ; Mlot1 (NP_106075.1), Mlot2 (Q986V5), Mesorhizobium loti ; Pmar1 (ZP_01852636.1) Planctomyces maris ; Rbal1 (Q7UJ69) Rhodopirelula baltica ; Selo1 (Q31QL1) Synechococcus elongatus ;...”
LIC11101 dihydroxy-acid dehydratase from Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
Aligns to 20:559 / 560 (96.4%), covers 99.8% of TIGR00110, 775.1 bits
B9E299 Dihydroxy-acid dehydratase from Clostridium kluyveri (strain NBRC 12016)
Aligns to 14:553 / 556 (97.1%), covers 99.8% of TIGR00110, 774.6 bits
BMMGA3_10275 dihydroxy-acid dehydratase from Bacillus methanolicus MGA3
Aligns to 20:558 / 570 (94.6%), covers 99.8% of TIGR00110, 773.7 bits
PITG_22685 dihydroxy-acid dehydratase, putative from Phytophthora infestans T30-4
Aligns to 55:596 / 596 (90.9%), covers 99.6% of TIGR00110, 771.7 bits
PHYSODRAFT_557322 hypothetical protein from Phytophthora sojae
Aligns to 55:596 / 596 (90.9%), covers 99.6% of TIGR00110, 771.2 bits
- Insights into the adaptive response of the plant-pathogenic oomycete Phytophthora capsici to the fungicide flumorph
Pang, Scientific reports 2016 - “...protein PHYSODRAFT_349787 Others 348683892 hypothetical protein PHYSODRAFT_353864 Others 348683825 putative dehydratase Others 348681277 hypothetical protein PHYSODRAFT_557322 Others 348673004 hypothetical protein PHYSODRAFT_354913 Others 348673003 hypothetical protein PHYSODRAFT_354912 Others 262105863 aldo/keto reductase family Others 262103226 succinate semialdehyde dehydrogenase Others 262099089 succinate dehydrogenase iron-sulfur protein Others 262098735 alcohol dehydrogenase,...”
ABH19_09600 dihydroxy-acid dehydratase from Leptospirillum sp. Group II 'CF-1'
Aligns to 18:558 / 558 (97.0%), covers 99.8% of TIGR00110, 770.6 bits
- A novel gene from the acidophilic bacterium Leptospirillum sp. CF-1 and its role in oxidative stress and chromate tolerance
Javier, Biological research 2022 - “...contained in an operon, here named the och operon, that also contains ABH19_09585, ABH19_09595 and ABH19_09600 genes. The expression of the och operon was significantly up-regulated in Leptospirillum sp. CF-1 exposed to 5mM K 2 CrO 4 for 15 and 30min. Genes of this operon potentially...”
- “...are found, while downstream, genes encoding a hypothetical protein (ABH19_09595), a possible dihydroxy-acid dehydratase (DHAD, ABH19_09600) and the translational factor Sua5 (ABH19_09605) were detected. The six candidate genes were analyzed to evaluate co-transcription using RT-PCR, in order to define whether ABH19_09590 is contained in an operon....”
Clocel_0493 dihydroxy-acid dehydratase from Clostridium cellulovorans 743B
Aligns to 11:550 / 552 (97.8%), covers 99.8% of TIGR00110, 769.9 bits
- Clostridium cellulovorans Proteomic Responses to Butanol Stress
Costa, Frontiers in microbiology 2021 - “...lysine (Clocel_1978, Clocel_3115), methionine (Clocel_1764, Clocel_2896, Clocel_3040), and branched chain amino acids (BCAA) (Clocel_1324, Clocel_1325, Clocel_0493) ( Table 1 and Supplementary Table 2 ). In addition, up-regulated proteins include four aminotransferases (Clocel_1948, Clocel_2059, Clocel_2390, Clocel_3812). The role of amino acids in the cellular stress response is...”
- “...of branched-chain amino acids (acetolactate synthase large subunit, Clocel_1324, ketol-acid reductoisomerase, Clocel_1325, and dihydroxy-acid dehydratase, Clocel_0493) could possibly refer to additional mechanisms to modulate cell membrane fluidity ( Mansilla et al., 2004 ). In fact, branched-chain amino acids are used as primers for the synthesis of...”
B2TIR2 Dihydroxy-acid dehydratase from Clostridium botulinum (strain Eklund 17B / Type B)
Aligns to 14:552 / 552 (97.6%), covers 99.8% of TIGR00110, 768.6 bits
XP_958280 dihydroxy-acid dehydratase from Neurospora crassa OR74A
Aligns to 54:596 / 596 (91.1%), covers 99.6% of TIGR00110, 768.0 bits
BIF_00467 dihydroxy-acid dehydratase from Bifidobacterium animalis subsp. lactis BB-12
Aligns to 16:609 / 619 (96.0%), covers 99.6% of TIGR00110, 767.9 bits
- Updated Genome Sequence for the Probiotic Bacterium Bifidobacterium animalis subsp. lactis BB-12
Jensen, Microbiology resource announcements 2021 - “...Intergenic(+236/+53) BIF_00072 / BIF_00341 1360042 19bp35bp Intergenic(+28/+7) BIF_00368 / BIF_01760 1376069 14bp33bp Intergenic(+38/+27) BIF_01846 / BIF_00467 1414041 CA A373D(G C CG A C) BIF_00934 1414072 1bp Coding(1,149/1,152nt) BIF_00934 1414079 +G Intergenic(+4/+74) BIF_00934 / BIF_00718 1414090 8bp28bp Intergenic(+15/+56) BIF_00934 / BIF_00718 1419213 +G Intergenic(+37/+331) BIF_00332 / BIF_02065...”
LMON_2054, LMRG_RS10020 dihydroxy-acid dehydratase from Listeria monocytogenes 10403S
lmo1983 similar to dihydroxy-acid dehydratase from Listeria monocytogenes EGD-e
LMRG_01131 dihydroxy-acid dehydratase from Listeria monocytogenes 10403S
Aligns to 14:558 / 564 (96.6%), covers 99.8% of TIGR00110, 767.8 bits
- Genome-wide identification of Listeria monocytogenes CodY-binding sites
Biswas, Molecular microbiology 2020 - “...LMRG_01636) (K D 5 nM), glnK (lmo1517, LMRG_RS07550, LMRG_01453) (K D 5 nM), ilvD (lmo1983, LMRG_RS10020, LMRG_01131) (K D 10 nM), ilvE (lmo0978, LMRG_RS04930, LMRG_02078) (K D 12.5 nM), mfd (lmo0214, LMRG_RS01035, LMRG_02636) (K D 12.5 nM), and lmo0849 ( LMRG_RS04250, LMRG_02272) (K D 12.5 nM)...”
- Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Hingston, PloS one 2017 - “...-0.01 LMON_1932 Predicted membrane protein hemolysin III + lmo1864 14 2.21 1.58 1.78 1.84 1.06 LMON_2054 Dihydroxy-acid dehydratase ilvD + lmo1983 4 * 3.52 3.89 3.34 4.29 3.62 LMON_2055 Acetolactate synthase large subunit ilvB + lmo1984 3/15 3.67 4.17 3.41 4.41 4.37 LMON_2056 Acetolactate synthase small...”
- “...5.06 2.55 -0.95 0.67 LMON_1936 Pyruvate,phosphate dikinase + lmo1867 20 4.31 2.17 1.90 0.47 2.16 LMON_2054 Dihydroxy-acid dehydratase ilvD + lmo1983 4 * 3.52 3.89 3.34 4.29 3.62 LMON_2055 Acetolactate synthase large subunit ilvB + lmo1984 3/15 3.67 4.17 3.41 4.41 4.37 LMON_2056 Acetolactate synthase small...”
- Genome-wide identification of Listeria monocytogenes CodY-binding sites
Biswas, Molecular microbiology 2020 - “...LMRG_RS11140, LMRG_01636) (K D 5 nM), glnK (lmo1517, LMRG_RS07550, LMRG_01453) (K D 5 nM), ilvD (lmo1983, LMRG_RS10020, LMRG_01131) (K D 10 nM), ilvE (lmo0978, LMRG_RS04930, LMRG_02078) (K D 12.5 nM), mfd (lmo0214, LMRG_RS01035, LMRG_02636) (K D 12.5 nM), and lmo0849 ( LMRG_RS04250, LMRG_02272) (K D 12.5...”
- Listeria monocytogenes σA Is Sufficient to Survive Gallbladder Bile Exposure
Boonmee, Frontiers in microbiology 2019 - “...LMRG_00826 lmo1375 Peptidase M20 2.07 0.00 LMRG_00942 lmo1489 LMRG_00945-LMRG_00939 RNA binding protein 2.22 0.00 LMRG_01131 lmo1983 ilvD ilv-leu Dihydroxy-acid dehydratase 2.62 0.01 LMRG_01132 lmo1984 ilvB Acetolactate synthase large subunit 3.12 0.00 LMRG_01134 lmo1986 ilvC Ketol-acid reductoisomerase 2.91 0.00 LMRG_01453 lmo1517 LMRG_01454-LMRG_01453 Nitrogen regulatory protein P-II 2.07...”
- Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Hingston, PloS one 2017 - “...hemolysin III + lmo1864 14 2.21 1.58 1.78 1.84 1.06 LMON_2054 Dihydroxy-acid dehydratase ilvD + lmo1983 4 * 3.52 3.89 3.34 4.29 3.62 LMON_2055 Acetolactate synthase large subunit ilvB + lmo1984 3/15 3.67 4.17 3.41 4.41 4.37 LMON_2056 Acetolactate synthase small subunit ilvH + lmo1985 15/1...”
- “...Pyruvate,phosphate dikinase + lmo1867 20 4.31 2.17 1.90 0.47 2.16 LMON_2054 Dihydroxy-acid dehydratase ilvD + lmo1983 4 * 3.52 3.89 3.34 4.29 3.62 LMON_2055 Acetolactate synthase large subunit ilvB + lmo1984 3/15 3.67 4.17 3.41 4.41 4.37 LMON_2056 Acetolactate synthase small subunit ilvH + lmo1985 15/1...”
- The multicopy sRNA LhrC controls expression of the oligopeptide-binding protein OppA in Listeria monocytogenes
Sievers, RNA biology 2015 - “...2.40 1.50 1.002 0.452 2.63 lmo1584 lmo1585 lmo1983 / ilvD hypothetical transport protein transcriptional regulator CD4C T cell-stimulating antigen, lipoprotein...”
- Pyruvate carboxylase plays a crucial role in carbon metabolism of extra- and intracellularly replicating Listeria monocytogenes
Schär, Journal of bacteriology 2010 - “...lmo1298 (glnR)e lmo1299 (glnAe lmo1516d lmo1517d lmo1634 lmo1983 (ilvD) lmo1984 (ilvB)b lmo1985 (ilvN)b lmo2064 lmo2192d lmo2193d lmo2195d lmo2360 lmo2374...”
- Intracellular gene expression profile of Listeria monocytogenes
Chatterjee, Infection and immunity 2006 - “...(encoded by ilv and leu) was highly enhanced (lmo1983 to lmo1991). The TnrA protein, a negative regulator of branched-chain-amino-acid expression in B....”
- Genome-wide identification of Listeria monocytogenes CodY-binding sites
Biswas, Molecular microbiology 2020 - “...(K D 5 nM), glnK (lmo1517, LMRG_RS07550, LMRG_01453) (K D 5 nM), ilvD (lmo1983, LMRG_RS10020, LMRG_01131) (K D 10 nM), ilvE (lmo0978, LMRG_RS04930, LMRG_02078) (K D 12.5 nM), mfd (lmo0214, LMRG_RS01035, LMRG_02636) (K D 12.5 nM), and lmo0849 ( LMRG_RS04250, LMRG_02272) (K D 12.5 nM) genes...”
- Listeria monocytogenes σA Is Sufficient to Survive Gallbladder Bile Exposure
Boonmee, Frontiers in microbiology 2019 - “...0.00 LMRG_00826 lmo1375 Peptidase M20 2.07 0.00 LMRG_00942 lmo1489 LMRG_00945-LMRG_00939 RNA binding protein 2.22 0.00 LMRG_01131 lmo1983 ilvD ilv-leu Dihydroxy-acid dehydratase 2.62 0.01 LMRG_01132 lmo1984 ilvB Acetolactate synthase large subunit 3.12 0.00 LMRG_01134 lmo1986 ilvC Ketol-acid reductoisomerase 2.91 0.00 LMRG_01453 lmo1517 LMRG_01454-LMRG_01453 Nitrogen regulatory protein P-II...”
- Controlled branched-chain amino acids auxotrophy in Listeria monocytogenes allows isoleucine to serve as a host signal and virulence effector
Brenner, PLoS genetics 2018 - “...and ilvD transcription pattern under varying BCAA concentrations. (A) Schematic representation of rli60 and ilvD (LMRG_01131 ) genomic region. A single promoter is indicated upstream to rli60 (-10 and -35 are marked as white boxes). Two putative CodY binding sites are indicated upstream to rli60 and...”
Q74BW7 Dihydroxy-acid dehydratase from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
GSU1912 dihydroxy-acid dehydratase from Geobacter sulfurreducens PCA
Aligns to 14:552 / 553 (97.5%), covers 99.8% of TIGR00110, 767.4 bits
- Multi-instance multilabel learning with weak-label for predicting protein function in electricigens
Wu, BioMed research international 2015 - “...Case Study Table 6 presents two example results. The first protein with the UniProt ID Q74BW7 from the Geobacter sulfurreducens organism has seven ground-truth labels: {GO:0008270, GO:0046872, GO:0000287, GO:0051539, GO:0030145, GO:0005506, GO:0004160}. After training examples with 80% weak-label ratios by different MIML methods, the trained model...”
- “...examples. Organism/UniProt ID Molecular function in UniProt Methods GO molecular function list Geobacter sulfurreducens / Q74BW7 (1) 4 iron, 4 sulfur cluster binding (2) Dihydroxy-acid dehydratase activity (3) Metal ion binding Ground truth GO:0008270 GO:0046872 GO:0000287 GO:0051539 GO:0030145 GO:0005506 GO:0004160 MIMLwel GO:0005524 GO:0008270 GO:0046872 GO:0000287 GO:0030145...”
- Evolution of electron transfer out of the cell: comparative genomics of six Geobacter genomes
Butler, BMC genomics 2010 - “...GSU0342 0.90 NADH dehydrogenase I, E subunit 39997952 GSU2860 0.90 translation elongation factor G 39997010 GSU1912 0.90 dihydroxy-acid dehydratase 39998398 GSU3309 0.90 hypothetical protein GSU3309 Identifying genes encoding cytochromes After the electron donors have been oxidized and the electrons have been transferred into the inner membrane,...”
SCO3345 dihydroxy-acid dehydratase from Streptomyces coelicolor A3(2)
Aligns to 17:613 / 617 (96.8%), covers 99.6% of TIGR00110, 766.7 bits
- The Onset of Tacrolimus Biosynthesis in Streptomyces tsukubaensis Is Dependent on the Intracellular Redox Status
Pires, Antibiotics (Basel, Switzerland) 2020 - “...subunit (SdhB) 0.50 1 STSU_30056 SCO1391 Phosphoenolpyruvate-protein phosphotransferase (EI component) 0.45 Amino acid metabolism STSU_14552 SCO3345 Dihydroxy-acid dehydratase (IlvD) 0.29 STSU_24776 SCO2528 2-isopropylmalate synthase (LeuA) 0.21 1 STSU_26189 SCO2198 Glutamine synthetase I (GlnA) 0.23 Hypothetical/uncharacterized proteins/not classified STSU_10084 SCO5389 Hypothetical protein 2.00 STSU_13630 SCO4637 Hypothetical protein...”
- Genome-Wide Mutagenesis Links Multiple Metabolic Pathways with Actinorhodin Production in Streptomyces coelicolor
Xu, Applied and environmental microbiology 2019 - “...genes involved in branched-chain amino acid metabolism, including three ACT upmodulators, SCO2528 ( leuA ), SCO3345 ( ilvD ), and SCO5512 ( ilvB ), and three ACT downmodulators, SCO5513 ( ilvN ), SCO5514 ( ilvC ), and SCO5522 ( leuB ); five genes involved in the...”
- “...SCO5522 ( leuB ) had increased ACT production, whereas mutants of SCO5512 ( ilvB ), SCO3345 ( ilvD ), and SCO2528 ( leuA ) had decreased ACT production. The ilvB , ilvC , ilvD , and leuA mutants were bald (deficient in developing aerial hyphae). Branched-chain...”
- Large-Scale Transposition Mutagenesis of Streptomyces coelicolor Identifies Hundreds of Genes Influencing Antibiotic Biosynthesis
Xu, Applied and environmental microbiology 2017 - “...amino acids affected RED production. Mutants of SCO3345 (ilvD), SCO2528 (leuA), and SCO5529 (leuA2) increased RED production, while mutants of SCO5553...”
- Genome-scale analysis reveals a role for NdgR in the thiol oxidative stress response in Streptomyces coelicolor
Kim, BMC genomics 2015 - “...* SCO2999 gdhB Glutamate dehydrogenase 0.0.2 Conserved in organism other than Escherichia coli *, I SCO3345 ilvD Dihydroxy acid dehydratase 3.1.21 Valine * SCO4164 cysA Putative thiosulfate sulfurtransferase 3.3.19 Sulfur metabolism * SCO4165 Hypothetical protein 0.0.2 Conserved in organism other than Escherichia coli SCO4193 Putative ATP/GTP-binding...”
- “...upstream region between two divergent genes is denoted as D. Five genes, metF (SCO2103), ilvD (SCO3345), ilvB (SCO5512), leuB (SCO5522), and ndgR - leuC intergenic region (SCO5552-SCO5553) were previously annotated as direct regulatory targets of NdgR by in vitro experiments such as electrophoretic mobility shift assay...”
- Genome-wide dynamics of a bacterial response to antibiotics that target the cell envelope
Hesketh, BMC genomics 2011 - “...branched chain amino acid (leucine, isoleucine and valine), lysine and methionine biosynthesis. Indeed, the ilvD (SCO3345), leuB (SCO5522) and leuC (SCO5553) genes for the biosynthesis of the branched chain amino acids leucine, valine and isoleucine were up to 7-fold up-regulated by moenomycin treatment (Additional file 14...”
Cj0013 dihydroxy-acid dehydratase from Campylobacter jejuni subsp. jejuni NCTC 11168
Aligns to 14:557 / 558 (97.5%), covers 99.8% of TIGR00110, 764.2 bits
- A Systematic Review of Campylobacter jejuni Vaccine Candidates for Chickens
Pumtang-On, Microorganisms 2021 - “...26 ] 60 10 8 CFU of Salmonella Enteritidis (SE) vectored vaccine expressing Omp18 protein (Cj0013), peptidoglycan associated lipoprotein of Salmonella (PAL of Salmonella ), and high mobility group box 1 protein (HMGB1), orally C. jejuni field strain (6.8) and Day 7 43 7.14 0.29 7.70...”
- “...61 10 8 CFU of SE vectored vaccine expressing HMGB1, PAL of Salmonella , and Cj0013, orally C. jejuni field strain (6.8) and Day 7 43 7.5 3 7.70 0.29 Non-significant 0.2 log10 reduction (non-significant) Yang et al. [ 58 ] 62 10 8 CFU of...”
- Identification of Campylobacter jejuni genes contributing to acid adaptation by transcriptional profiling and genome-wide mutagenesis
Reid, Applied and environmental microbiology 2008 - “...(nuoI) (nuoG) (gltA) Amino acid biosynthesis and transport Cj0007 (gltB) Cj0013 (ilvD) Cj1015c (livG) DOWN (2, 12) ND UP (4) DOWN (4) DOWN (5.5) UP (12, 20) UP...”
- Signature-tagged transposon mutagenesis studies demonstrate the dynamic nature of cecal colonization of 2-week-old chickens by Campylobacter jejuni
Grant, Applied and environmental microbiology 2005 - “...Intergenic cj1191-dctA cj1194 HS41.24a cj0337c cj0631c cj0970 cj1471c cj0013 cj1544c cj0948c --b cj1716c cj0893c --c cj1633 Unmatched cj1468 cj1256c cj1565c a b...”
FOIG_06470 dihydroxy-acid dehydratase, mitochondrial from Fusarium odoratissimum NRRL 54006
Aligns to 52:594 / 598 (90.8%), covers 99.6% of TIGR00110, 761.1 bits
BBA_09252 dihydroxy-acid dehydratase from Beauveria bassiana ARSEF 2860
Aligns to 62:604 / 606 (89.6%), covers 99.6% of TIGR00110, 760.9 bits
- Unveiling a Novel Role of Cdc42 in Pyruvate Metabolism Pathway to Mediate Insecticidal Activity of Beauveria bassiana
Guan, Journal of fungi (Basel, Switzerland) 2022 - “...implicated an increase in pyruvate accumulation in the cdc42 mutant. Moreover, several upregulated genes (BBA_00790, BBA_09252, BBA_01687, BBA_04309, and BBA_08608) were involved in the participation of pyruvate in tricarboxylic acid (TCA) cycle and/or valine, leucine, and isoleucine biosynthesis. Three other upregulated genes (BBA_ 08607, BBA_02336, and...”
VDAG_04620 dihydroxy-acid dehydratase from Verticillium dahliae VdLs.17
Aligns to 57:599 / 601 (90.3%), covers 99.6% of TIGR00110, 756.3 bits
- The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen Verticillium dahliae
Wang, mSphere 2018 - “...homoaconitase; hemA ( VDAG_06343 ), encoding aminolevulinic acid synthase; VDAG_00564 , encoding isopropylmalate dehydratase; and VDAG_04620 , encoding dihydroxy acid dehydratase. After a 45-min shift from iron-limiting to iron-replete conditions, deletion of VdHapX impairs the transcriptional activation of VDAG_02332 , VDAG_08540 , VDAG_06343 , and VDAG_04620...”
- “...+Fe). Transcript levels of acoA , lysF , hemA , cycA , VDAG_00564 , and VDAG_04620 were analyzed under the different iron conditions shown. Averages of the gene expression values were normalized against the V. dahliae -tubulin gene. Error bars represent standard deviations. Asterisks represent signicant...”
FPSE_05147 hypothetical protein from Fusarium pseudograminearum CS3096
Aligns to 52:594 / 598 (90.8%), covers 99.6% of TIGR00110, 755.4 bits
- Histone H3 N-Terminal Lysine Acetylation Governs Fungal Growth, Conidiation, and Pathogenicity through Regulating Gene Expression in Fusarium pseudograminearum
Jiang, Journal of fungi (Basel, Switzerland) 2024 - “...and FpH3 K18R mutants ( Supplementary Tables S4S6 ) [ 38 ]. Additionally, dihydroxyacid dehydratase (FPSE_05147, FpILV3A ), hexokinase (FPSE_04405, FpHXK1 ), and acetohydroxyacid synthase (FPSE_00996, FpILV6 ), which play vital roles in growth, conidiation, and pathogenicity, are all downregulated in the FpH3 K9R , FpH3...”
FGSG_02056 dihydroxy-acid dehydratase from Fusarium graminearum PH-1
Aligns to 58:600 / 604 (89.9%), covers 99.6% of TIGR00110, 755.1 bits
SPAC17G8.06c dihydroxy-acid dehydratase from Schizosaccharomyces pombe
Aligns to 52:596 / 598 (91.1%), covers 99.6% of TIGR00110, 754.0 bits
- Response to leucine in Schizosaccharomyces pombe (fission yeast)
Ohtsuka, FEMS yeast research 2022 - “.... 1974 ). In S. pombe , this enzyme is predicted to be encoded by SPAC17G8.06c, but this prediction has not been confirmed. Therefore, we investigated whether S. cerevisiae ILV3 encoding dihydroxy-acid dehydratase can complement this gene (Fig. 2 ). The deletion mutant of SPAC17G8.06c can...”
- “...also essential for the growth of S. pombe in EMM. The defective growth of the SPAC17G8.06c mutant was complemented not only by the expression of S. pombe SPAC17G8.06c itself but also by the S. cerevisiae ILV3 . This finding suggests that S. pombe SPAC17G8.06c is a...”
- Posttranslational Arginylation Enzyme Arginyltransferase1 Shows Genetic Interactions With Specific Cellular Pathways in vivo
Wiley, Frontiers in physiology 2020 - “...protein L18 RPL18B,RPL18A RPL18 rpl3702 SPCC1223.05c 0.00161788 3.15266 60S ribosomal protein L37 (predicted) RPL37B,RPL37A RPL37 SPAC17G8.06c 0.00198848 3.09195 Dihydroxy-acid dehydratase (predicted) ILV3 his7 SPBC29A3.02c 0.0021838 3.06403 Phosphoribosyl-AMP cyclohydrolase/phosphoribosyl- ATP pyrophosphohydrolase His7 HIS4 lys9 SPBC3B8.03 0.0023364 3.04376 Saccharopine dehydrogenase LYS9 AASS his5 SPBC21H7.07c 0.002481 3.02565 Imidazoleglycerol-phosphate dehydratase...”
- “...hits list Mitochondrion (mitochondria) GO:0005739 428 19 mpn1, SPACUNK4.13c, lip2, gor2, hmt2, sod2, SPAC14C4.01c, qcr8, SPAC17G8.06c, cox6, oma1, cys2, SPBC1271.14, SPBC1703.13c, atp3, atp15, coq7, rps1802, ppr5 Mitochondria inner membrane GO:0005743 71 4 oma1, SPBC1703.13c, coq7, atp15 The numbers of those genes in the effective library (non-sterile)...”
- Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells
Brault, G3 (Bethesda, Md.) 2016 - “...synthase Glt1 (predicted) 2.982 3.254 913 a , 881 a , 266 a , 199 SPAC17G8.06c Dihydroxy-acid dehydratase (predicted) 2.781 3.534 327, 284 a SPBC21H7.07c his5 + Imidazoleglycerol-phosphate dehydratase His5 2.636 2.623 994, 269, 165 a SPAC13G7.06 met16 + Phosphoadenosine phosphosulfate reductase 2.463 2.461 820 a...”
- Metabolic remodeling in iron-deficient fungi
Philpott, Biochimica et biophysica acta 2012 - “...GLT1 Afu1g07380 glt1 -isopropylmalate isomerase (Leu) LEU1 Afu2g11260 leu2 dihydroxy acid dehydratase (Ile/Val) ILV3 Afu2g14210 SPAC17G8.06C Homoaconitase (Lys) LYS4 lysF lys2 Fe-S cluster biosynthesis iron sulfur assembly protein ISA1 Afu4g10690 isa1 TCA cycle succinate dehydrogenase, flavoprotein subunit SDH1 Afu3g07810 sdh1 succinate dehydrogenase, cytochrome b560 subunit SDH3...”
- Key function for the CCAAT-binding factor Php4 to regulate gene expression in response to iron deficiency in fission yeast
Mercier, Eukaryotic cell 2008 - “...516, 387, 236 16.8 3.5 21.9 3.1 SPAC17G8.06c SPAC343.16 SPBC21H7.07c SPAC1782.11 SPBC27.08c SPBC1709.19c Fe related SPBC1683.10c SPAC23E2.01 pcl1 fep1 Ferrous...”
Q7UJ69 Dihydroxy-acid dehydratase from Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)
Aligns to 28:567 / 567 (95.2%), covers 99.6% of TIGR00110, 752.9 bits
A8IX80 dihydroxy-acid dehydratase from Chlamydomonas reinhardtii
Aligns to 62:604 / 604 (89.9%), covers 99.6% of TIGR00110, 750.8 bits
ZP_01094431 dihydroxy-acid dehydratase from Blastopirellula marina DSM 3645
Aligns to 20:560 / 560 (96.6%), covers 99.6% of TIGR00110, 749.1 bits
- The Aspergillus fumigatus dihydroxyacid dehydratase Ilv3A/IlvC is required for full virulence
Oliver, PloS one 2012 - “...(Q1ILZ0) Acidobacteria bacterium ; Aura1 (ZP_01227770) Aurantimonas manganoxydans ; Bbro1 (Q7WKV5) Bordetella bronchiseptica ; Bmar1 (ZP_01094431) Blastopirellula marina ; Bpar1 (NP_883684.1) Bordetella parapertussis ; Cagg1 (AOH553_9CHLR) Chloroflexus aggregans ; Cvib1 (Q9A8D3) Caulobacter vibroides ; Lint1 (Q8F219) Leptospira interrogans ; Meth1 (ZP_01850751.1) Methylobacterium sp ; Mlot1 (NP_106075.1),...”
ilvD / Q0K7F8 dihydroxyacid dehydratase (EC 4.2.1.9) from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (see paper)
Q0K7F8 dihydroxy-acid dehydratase (EC 4.2.1.9) from Cupriavidus necator (see paper)
Aligns to 18:557 / 557 (96.9%), covers 99.8% of TIGR00110, 748.2 bits
BL1788 dihydroxy-acid dehydratase from Bifidobacterium longum NCC2705
Aligns to 17:610 / 620 (95.8%), covers 99.6% of TIGR00110, 747.2 bits
SXYL_02469 dihydroxy-acid dehydratase from Staphylococcus xylosus
Aligns to 29:566 / 566 (95.1%), covers 99.8% of TIGR00110, 744.9 bits
- Transcriptomic Analysis of Staphylococcus xylosus in Solid Dairy Matrix Reveals an Aerobic Lifestyle Adapted to Rind
Leroy, Microorganisms 2020 - “...SXYL_00867-69 ilvAleuDCB Leucine, valine, isoleucine biosynthesis 4.7 * 2.9 * SXYL_00870 leuB 3-isopropylmalate dehydrogenase 2.5 SXYL_02469 ilvD2 Dihydroxy-acid dehydratase 3.0 3.0 Glycine SXYL_01317-19 gcvTPAPB Glycine metabolism 4.7 * 3.1 * Tryptophan SXYL_01497 trpA Tryptophan synthase alpha chain 5.4 SXYL_01498-503 trpBFCDGE Tryptophan biosynthesis 6.3 * 6.2 *...”
- Adaptation of Staphylococcus xylosus to Nutrients and Osmotic Stress in a Salted Meat Model
Vermassen, Frontiers in microbiology 2016 - “...isoleucine SXYL_00867-75 ilvA, leuDCBA, ilvCNBD1 Valine, leucine, isoleucine biosynthesis 0.3 * 0.1 * 0.1 * SXYL_02469 ilvD2 Dihydroxy-acid dehydratase 0.2 0.1 0.1 SXYL_01337-40 lpdA, bkdA1A2 alpha-keto acid dehydrogenase complex 4.9 * 3.7 * 2.8 * Tryptophan, phenylalanine, tyrosine SXYL_01383-85 aroABC Aromatic acid biosynthesis 0.3 * 0.3...”
ABO_0180 dihydroxy-acid dehydratase from Alcanivorax borkumensis SK2
Aligns to 24:560 / 561 (95.7%), covers 99.8% of TIGR00110, 743.2 bits
Q8XWR1 Dihydroxy-acid dehydratase from Ralstonia nicotianae (strain ATCC BAA-1114 / GMI1000)
Aligns to 18:557 / 557 (96.9%), covers 99.8% of TIGR00110, 742.9 bits
A0A481UJA7 dihydroxy-acid dehydratase (EC 4.2.1.9) from Hevea brasiliensis (see paper)
Aligns to 74:614 / 614 (88.1%), covers 99.6% of TIGR00110, 742.6 bits
G7IRL3 dihydroxy-acid dehydratase from Medicago truncatula
Aligns to 58:598 / 598 (90.5%), covers 99.6% of TIGR00110, 740.8 bits
- Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature
Arshad, BMC genomics 2020 - “...MTR_5g096970 3.13 0.0035 Carboxy-terminal TIM barrel domain enolase G7JMP4 MTR_4g104300 3.10 0.0345 F-box/RNI/FBD-like domain protein G7IRL3 MTR_2g089340 2.95 0.0127 Dihydroxyacid dehydratase G7KG90 MTR_5g012030 2.86 0.0127 Putative Heat shock chaperonin-binding A0A072VBG9 MTR_2g084715 2.84 0.0018 Putative transcription factor C3H family G7L4S2 MTR_7g088490 2.77 0.0327 Proteasome subunit beta A0A072UGC6...”
- “...0.0157 Sterol regulatory element-binding protein G7IF74 MTR_1g088640 4.31 0.0048 Putative universal stress protein A a G7IRL3 MTR_2g089340 3.60 0.0076 Dihydroxyacid dehydratase G7LGJ8 MTR_8g095680 3.33 0.0173 Calnexin 2 a G7KWU8 MTR_7g024390 3.24 0.0160 Heat shock cognate 70kDa protein a G7L491 MTR_7g012820 2.70 0.0080 Casein lytic proteinase B3...”
ACIAD3636 putative dihydroxyacid dehydratase (ilvD-like) from Acinetobacter sp. ADP1
Aligns to 21:557 / 561 (95.7%), covers 99.8% of TIGR00110, 738.0 bits
Sb07g024070 No description from Sorghum bicolor
Aligns to 51:591 / 591 (91.5%), covers 99.6% of TIGR00110, 736.0 bits
WP_011692381 dihydroxy-acid dehydratase from Arthrobacter sp. FB24
Aligns to 30:572 / 573 (94.8%), covers 99.1% of TIGR00110, 735.6 bits
ILVD_ARATH / Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q9LIR4 dihydroxy-acid dehydratase (EC 4.2.1.9) from Arabidopsis thaliana (see 2 papers)
NP_189036 dehydratase family from Arabidopsis thaliana
AT3G23940 dehydratase family from Arabidopsis thaliana
Aligns to 68:608 / 608 (89.0%), covers 99.6% of TIGR00110, 734.0 bits
- function: Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo- 3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3- dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.
catalytic activity: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O (RHEA:24809)
catalytic activity: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2- oxopentanoate + H2O (RHEA:27694)
cofactor: [2Fe-2S] cluster (Binds 1 [2Fe-2S] cluster per subunit.)
cofactor: Mg(2+) - Dihydroxyacid dehydratase is important for gametophyte development and disruption causes increased susceptibility to salinity stress in Arabidopsis.
Zhang, Journal of experimental botany 2015 - GeneRIF: Dihydroxyacid dehydratase (DHAD) is highly expressed in most vegetative and reproductive tissues. It is an essential gene, and complete disruption caused partial sterility in both male and female gametophyte phases. In addition, reduced expression of DHAD in knockdown mutants resulted in a reduction in the accumulation of all three BCAAs in roots and, as a consequence, led to a shorter root phenotype.[DHAD]
- Structure-Guided Modulation of the Catalytic Properties of [2Fe-2S]-Dependent Dehydratases
Melse, Chembiochem : a European journal of chemical biology 2022 - “...A0A4R3LQ44, Ft DHAD: A0A1I2J0Y3, Ss DHAD: Q97UB2, Rl ArDT: B5ZZ34, Mt DHAD: A0A0E8UWV6, At DHAD: Q9LIR4, Cc XylDHT: Q9A9Z2. Conflict of interest The authors declare no conflict of interest. 1 Supporting information As a service to our authors and readers, this journal provides supporting information supplied...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...decarboxylase 2 56 Q94A94, At5g11880 Diaminopimelate epimerase 86 Q9LFG2, At3g53580 Dihydroxy-acid dehydratase 30, 43, 45 Q9LIR4, At3g23940 Glutamine synthetase 55 Q43127, At5g35630 Homoserine kinase 83, 85 Q8L7R2, At2g17265 Imidazole glycerol phosphate synthase hisHF 4346, 56 Q9SZ30, At4g26900 Ketol-acid reductoisomerase 36, 37 Q05758, At3g58610 Ornithine carbamoyltransferase 85...”
- Proteomic Analysis of Arabidopsis pldα1 Mutants Revealed an Important Role of Phospholipase D Alpha 1 in Chloroplast Biogenesis
Takáč, Frontiers in plant science 2019 - “...At5g47840 Adenylate kinase 2, chloroplastic mutant unique mutant unique n.a. n.a. 11.01/8.51 3/2 19.43/14.49 3/4 Q9LIR4 At3g23940 Dihydroxy-acid dehydratase, chloroplastic Unique in Col-0 0.31 n.a. 0.04 7.31/7.59 2/2 6.09/6.09 3/5 Q8L940 At5g01410 Pyridoxal 5'-phosphate synthase subunit PDX1.3 1.49 1.85 0.053 0.018 23.65/23.94 6/6 29.77/29.77 28/28 Q9LEU8...”
- Involvement of a universal amino acid synthesis impediment in cytoplasmic male sterility in pepper
Fang, Scientific reports 2016 - “...peptide fragments were derived from the conserved protein sequences of the UniProt accessions Q9C5U8 (HDH), Q9LIR4 (DAD), Q9SIE1 (ATAAT), P47999 (CS), P54887 (P5CS), Q9LV03 (GS), and CAC80377 (GAPDH). Each peptide, along with an additional N-terminal cysteine (not part of the protein sequence), was synthesized and purified...”
- “...1.850.22 P54887 Delta-1-pyrroline-5-carboxylate synthase A 98.41 2 717 5.89 77.70 1 0.910.11 0.330.09 0.420.03 0.360.03 Q9LIR4 Dihydroxy-acid dehydratase 109.82 2 608 5.44 61.06 1 1.530.33 0.620.08 0.420.05 0.440.04 Q9SI75 Elongation factor G 430.06 4 783 5.06 77.67 1 3.240.12 1.350.23 1.840.20 2.600.15 Q9C9C4 Enolase 1 125.74...”
- Structural Bases of Dihydroxy Acid Dehydratase Inhibition and Biodesign for Self-Resistance
Zang, Biodesign research 2024 (no snippet) - Molecular mechanisms of resistance to Myzus persicae conferred by the peach Rm2 gene: A multi-omics view
Le, Frontiers in plant science 2022 - “...2.60E-02 ALS; Acetolactate synthase Prupe_3G094300 AT1G80560 82 Yes -1.5 4.30E-07 IMDH2; 3-isopropylmalate dehydrogenase 2 Prupe_1G003100 AT3G23940 82 Yes -3.2 7.70E-21 DHAD; Dihydroxy-acid dehydratase Prupe_1G416900 AT3G49680 59 No 3.0 1.70E-03 BCAT3; Branched-chain-amino-acid aminotransferase 3 Lysine metabolism Prupe_8G007000 AT1G31230 80 Yes -2.7 8.60E-10 AKHSDH1;Aspartokinase/homoserine dehydrogenase 1 Prupe_1G334400 AT1G14810...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...2 56 Q94A94, At5g11880 Diaminopimelate epimerase 86 Q9LFG2, At3g53580 Dihydroxy-acid dehydratase 30, 43, 45 Q9LIR4, At3g23940 Glutamine synthetase 55 Q43127, At5g35630 Homoserine kinase 83, 85 Q8L7R2, At2g17265 Imidazole glycerol phosphate synthase hisHF 4346, 56 Q9SZ30, At4g26900 Ketol-acid reductoisomerase 36, 37 Q05758, At3g58610 Ornithine carbamoyltransferase 85 O50039,...”
- “...parallel pathway towards L-valine and L-isoleucine biosynthesis [ 22 , 29 ]. Dihydroxyacid dehydratase (DHAD, At3g23940), performing the third step in synthesis of L-isoleucine from 2-oxobutanoate or synthesis of L-valine from two molecules of pyruvate [ 30 ], was identified in spots 30, 43 and 45....”
- A Global Proteomic Approach Sheds New Light on Potential Iron-Sulfur Client Proteins of the Chloroplastic Maturation Factor NFU3
Berger, International journal of molecular sciences 2020 - “...J protein C82 At4g29890 CMO n.d. n.q. rieske 2Fe/2S Glycine betaine biosynthesis Choline monooxygenase (putative) At3g23940 DHAD n.v. n.v. 2Fe2S Branched chain amino acid biosynthesis Dihydroxyacid dehydratase At1g03055 DWARF27.1 n.d. n.q. 4Fe4S (?) Strigolactone biosynthesis DWARF27.1 At1g64680 DWARF27.2 1.05 * n.q. 4Fe4S (?) Unknown DWARF27.2 At4g01995...”
- Redox Conformation-Specific Protein-Protein Interactions of the 2-Cysteine Peroxiredoxin in Arabidopsis
Liebthal, Antioxidants (Basel, Switzerland) 2020 - “...60S ribosomal protein L26-1 (AT3G49910) Glutathione S-transferase F8 (AT2G47730) ATPase alpha subunit (AtCg00120) Dihydroxy-acid dehydratase (AT3G23940) PLAT domain-containing protein 1 (AT4G39730) Photosystem I reaction center subunit II-1 (AT4G02770) Monodehydroascorbate reductase 6 (AT1G63940) E-Z type HEAT repeat- protein (AT3G62530) Chlorophyll a-b binding protein 2 (AT1G29920) Protease Do-like...”
- Proteomic Analysis of Arabidopsis pldα1 Mutants Revealed an Important Role of Phospholipase D Alpha 1 in Chloroplast Biogenesis
Takáč, Frontiers in plant science 2019 - “...Adenylate kinase 2, chloroplastic mutant unique mutant unique n.a. n.a. 11.01/8.51 3/2 19.43/14.49 3/4 Q9LIR4 At3g23940 Dihydroxy-acid dehydratase, chloroplastic Unique in Col-0 0.31 n.a. 0.04 7.31/7.59 2/2 6.09/6.09 3/5 Q8L940 At5g01410 Pyridoxal 5'-phosphate synthase subunit PDX1.3 1.49 1.85 0.053 0.018 23.65/23.94 6/6 29.77/29.77 28/28 Q9LEU8 At5g10920...”
- Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action
Yan, Nature 2018 - “...we expressed and purified housekeeping DHAD from both A. terreus (XP_001208445.1, fDHAD) and A. thaliana (AT3G23940, pDHAD), as well as the putative self-resistance enzyme AstD ( Supplementary Fig. 6 ). Both housekeeping DHAD enzymes converted dihydroxyisovalerate to ketoisovalerate (pDHAD: k cat = 1.2 sec 1 ,...”
- “...primers pDHAD-pET-F and pDHAD-pET-R were used to amplify a 1.7 kb DNA fragment containing pdhad (AT3G23940). The PCR product was cloned into pET28a using Nhe I and Not I restriction sites. The resulting plasmid pDHAD-pET was transformed into E.coli BL21 (DE3) to give TY08. To express...”
- Function and maturation of the Fe-S center in dihydroxyacid dehydratase from Arabidopsis
Gao, The Journal of biological chemistry 2018 - “...purchased from GE Healthcare. Plasmid construction The AtDHAD (At3g23940) coding sequence was amplified by PCR from rosette cDNAs using the following primers:...”
- More
B4FWX5 dihydroxy-acid dehydratase from Zea mays
Aligns to 51:591 / 591 (91.5%), covers 99.6% of TIGR00110, 731.7 bits
- Quantitative iTRAQ-based proteomic analysis of phosphoproteins and ABA-regulated phosphoproteins in maize leaves under osmotic stress
Hu, Scientific reports 2015 - “...and ribosome, which had the second greatest number of proteins (B8A367, C0HHU2, B6TS38, C0HIV2, B4FRM3, B4FWX5, C0PKN2, B6TPG2, B4FCE7, O04014, B4FCK4, B4FWI0), and only B4FWI0 was not regulated by ABA under osmotic stress. Moreover, the signaling pathways related to photosynthesis, such as carbon fixation in photosynthetic...”
8hs0A / Q9LIR4 The mutant structure of dhad v178w
Aligns to 30:570 / 570 (94.9%), covers 99.6% of TIGR00110, 730.2 bits
- Ligands: fe2/s2 (inorganic) cluster; magnesium ion (8hs0A)
ILVC_ASPFU / Q4X099 Dihydroxy-acid dehydratase ilvC, mitochondrial; DHAD ilvC; EC 4.2.1.9 from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
AFUA_2G14210, Afu2g14210 dihydroxy acid dehydratase Ilv3, putative from Aspergillus fumigatus Af293
Aligns to 52:604 / 606 (91.3%), covers 99.6% of TIGR00110, 728.9 bits
- function: Dihydroxyacid dehydratase that catalyzes the third step in the common pathway leading to biosynthesis of branched-chain amino acids (PubMed:23028460). Catalyzes the dehydration of (2R,3R)-2,3- dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2- oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3- dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3- methylbutanoate (2-oxoisovalerate), the penultimate precursor to L- isoleucine and L-valine, respectively (By similarity). IlvC and the branched-chain amino acid biosynthesis are crucial for virulence and may be a potential target to develop antifungal agents (PubMed:23028460).
catalytic activity: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O (RHEA:24809)
catalytic activity: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2- oxopentanoate + H2O (RHEA:27694)
cofactor: [2Fe-2S] cluster (Binds 1 [2Fe-2S] cluster per subunit.)
cofactor: Mg(2+)
disruption phenotype: Requires supplementation with isoleucine and valine for growth (PubMed:23028460). Results in considerably lower kidney tissue burden in murine infection models (PubMed:23028460). - Assembly and disassembly of Aspergillus fumigatus conidial rodlets
Valsecchi, Cell surface (Amsterdam, Netherlands) 2019 - “...AFUB_043810 AFUA_3G04160 B0XZB5 Ser/Thr protein phosphatase family Yes 0.51 14.40 626 71.262 377700 1.3 AFUB_029830 AFUA_2G14210 B0XTT1 Mitochondrial dihydroxy acid dehydratase, putative No 0.51 12.60 642 68.256 371027 5.0 AFUB_098310 AFUA_4G04690 B0YE87 Uncharacterized protein No 0.73 18.70 209 22.594 333057 1.0 AFUB_025060 AFUA_2G09180 B0XRW4 Coatomer subunit...”
- The Aspergillus fumigatus dihydroxyacid dehydratase Ilv3A/IlvC is required for full virulence
Oliver, PloS one 2012 - “...the filamentous fungus Aspergillus fumigatus by homology to Saccharomyces cerevisiae ILV3. Two of these genes, AFUA_2G14210 and AFUA_1G03550, initially designated AfIlv3A and AfIlv3B for this study, clustered in the same group as S. cerevisiae ILV3 following phylogenetic analysis. To investigate the functions of these genes, AfIlv3A...”
- “...for branched-chain amino acid synthesis in A. fumigatus . A recombinant AfIlv3A protein derived from AFUA_2G14210 was shown to have DHAD activity in an in vitro assay, confirming that AfIlv3A is a DHAD. In addition we show that mutants lacking AfIlv3A and ilv3B exhibit reduced levels...”
- Metabolic remodeling in iron-deficient fungi
Philpott, Biochimica et biophysica acta 2012 - “...(Glu) GLT1 Afu1g07380 glt1 -isopropylmalate isomerase (Leu) LEU1 Afu2g11260 leu2 dihydroxy acid dehydratase (Ile/Val) ILV3 Afu2g14210 SPAC17G8.06C Homoaconitase (Lys) LYS4 lysF lys2 Fe-S cluster biosynthesis iron sulfur assembly protein ISA1 Afu4g10690 isa1 TCA cycle succinate dehydrogenase, flavoprotein subunit SDH1 Afu3g07810 sdh1 succinate dehydrogenase, cytochrome b560 subunit...”
B0XTT1 dihydroxy-acid dehydratase from Aspergillus fumigatus (strain CBS 144.89 / FGSC A1163 / CEA10)
Aligns to 88:640 / 642 (86.1%), covers 99.6% of TIGR00110, 728.6 bits
- Assembly and disassembly of Aspergillus fumigatus conidial rodlets
Valsecchi, Cell surface (Amsterdam, Netherlands) 2019 - “...AFUA_3G04160 B0XZB5 Ser/Thr protein phosphatase family Yes 0.51 14.40 626 71.262 377700 1.3 AFUB_029830 AFUA_2G14210 B0XTT1 Mitochondrial dihydroxy acid dehydratase, putative No 0.51 12.60 642 68.256 371027 5.0 AFUB_098310 AFUA_4G04690 B0YE87 Uncharacterized protein No 0.73 18.70 209 22.594 333057 1.0 AFUB_025060 AFUA_2G09180 B0XRW4 Coatomer subunit zeta,...”
- A murine inhalation model to characterize pulmonary exposure to dry Aspergillus fumigatus conidia
Buskirk, PloS one 2014 - “...Putative, Mitochondrial aconitate hydratase Q4WLN1 85.48/6.25 8 9.91 3 a) Putative, Mitochondrial dihydroxy acid dehydratase B0XTT1 68.21/7.78 10 13.08 b) Phosphoglucomutase PgmA B0XXA2 60.46/6.30 14 16.58 c) Putative, Pyruvate decarboxylase PdcA B0XXN9 62.96/6.07 3 10.72 d) Putative, GMC oxidoreductase Q4WFN7 72.10/7.08 3 7.58 4 a) Putative,...”
An14g03280 uncharacterized protein from Aspergillus niger
Aligns to 61:613 / 615 (89.9%), covers 99.6% of TIGR00110, 725.7 bits
SMU_RS09725 dihydroxy-acid dehydratase from Streptococcus mutans UA159
Q8DRT7 Dihydroxy-acid dehydratase from Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Aligns to 24:562 / 571 (94.4%), covers 99.8% of TIGR00110, 724.5 bits
PADG_07241 dihydroxy-acid dehydratase from Paracoccidioides brasiliensis Pb18
Aligns to 57:609 / 610 (90.7%), covers 99.6% of TIGR00110, 723.7 bits
PAAG_00014 dihydroxy-acid dehydratase from Paracoccidioides lutzii Pb01
Aligns to 58:610 / 611 (90.5%), covers 99.6% of TIGR00110, 722.7 bits
- Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
Bailão, PLoS neglected tropical diseases 2014 - “...Methionine biosynthesis 1 PAAG_08166 4-hydroxyphenylpyruvate dioxygenase 203.44 3.00 *** 1.13.11.27 Tyrosine and phenylalanine degradation 1 PAAG_00014 dihydroxy-acid dehydratase 312.52 10.83 0.73 4.2.1.9 Valine and isoleucine biosynthesis 2 PAAG_02554 3-hydroxyisobutyryl-CoA hydrolase 402.87 9.75 0.58 3.1.2.4 Valine degradation 2 PAAG_01194 2-oxoisovalerate dehydrogenase subunit beta 219.86 6.00 *** 1.2.4.4...”
Q97UB2 dihydroxy-acid dehydratase (EC 4.2.1.9) from Saccharolobus solfataricus (see 2 papers)
SSO3107 Dihydroxy-acid dehydratase (ilvD) from Sulfolobus solfataricus P2
Aligns to 18:557 / 558 (96.8%), covers 99.8% of TIGR00110, 716.3 bits
- Metabolic reconstruction and experimental verification of glucose utilization in Desulfurococcus amylolyticus DSM 16532
Reischl, Folia microbiologica 2018 - “...in S. solfataricus , e.g., SSO3124, SSO3117 and SSO3118 (Peng et al. 2011 ) or SSO3107, SSO1303 (Brouns et al. 2006 ), could not be detected in the genome of D. amylolyticus . Only a homolog of the 2-keto-3-deoxy-D-arabinonate dehydratase (COG3970), which is responsible for arabinose...”
- Structure-Guided Modulation of the Catalytic Properties of [2Fe-2S]-Dependent Dehydratases
Melse, Chembiochem : a European journal of chemical biology 2022 - “...of enzymes discussed in this study (UniProtKB); Pu DHT: A0A4R3LQ44, Ft DHAD: A0A1I2J0Y3, Ss DHAD: Q97UB2, Rl ArDT: B5ZZ34, Mt DHAD: A0A0E8UWV6, At DHAD: Q9LIR4, Cc XylDHT: Q9A9Z2. Conflict of interest The authors declare no conflict of interest. 1 Supporting information As a service to our...”
- The Aspergillus fumigatus dihydroxyacid dehydratase Ilv3A/IlvC is required for full virulence.
Oliver, PloS one 2012 - “...Synechococcus elongatus ; Smut1 (Q8DRT7) Streptococcus mutans ; Srub1 (Q2SOM3) Salinabacter ruber ; ARCHAEBACTERIA: Ssol1 (Q97UB2) Sulfolobus solfataricus . The scale bar corresponds to the branch length for an expected number of 0.1 substitutions per site. Group 1, which contained S. cerevisiae ILV3 also contained A....”
Q1ILZ0 Dihydroxy-acid dehydratase from Koribacter versatilis (strain Ellin345)
Aligns to 23:561 / 573 (94.1%), covers 99.6% of TIGR00110, 715.9 bits
- The Aspergillus fumigatus dihydroxyacid dehydratase Ilv3A/IlvC is required for full virulence.
Oliver, PloS one 2012 - “...Spom1 (ILV3_SCHPO), Schizosaccharomyces pombe ; Umay1 (UM05740.1), Umay2 (UM02980.1), Ustilago maydis . BACTERIA: ; Abac1 (Q1ILZ0) Acidobacteria bacterium ; Aura1 (ZP_01227770) Aurantimonas manganoxydans ; Bbro1 (Q7WKV5) Bordetella bronchiseptica ; Bmar1 (ZP_01094431) Blastopirellula marina ; Bpar1 (NP_883684.1) Bordetella parapertussis ; Cagg1 (AOH553_9CHLR) Chloroflexus aggregans ; Cvib1 (Q9A8D3)...”
SP_2126 dihydroxy-acid dehydratase from Streptococcus pneumoniae TIGR4
spr1935 Dihydroxyacid dehydratase from Streptococcus pneumoniae R6
SPD_1956 dihydroxy-acid dehydratase from Streptococcus pneumoniae D39
Aligns to 20:558 / 567 (95.1%), covers 99.8% of TIGR00110, 712.6 bits
- Biofilm and planktonic pneumococci demonstrate disparate immunoreactivity to human convalescent sera
Sanchez, BMC microbiology 2011 - “...2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase dapH (SP_2097) 0 8 Aspartate--ammonia ligase asnA (SP_1970) 3 0 Dihydroxy-acid dehydratase ilvD (SP_2126) 0 7 ATP synthase subunit alpha atpA (SP_1510) 4 21 ATP synthase subunit beta atpD (SP_1508) 9 18 ATP synthase gamma chain atpG (SP_1509) 0 8 Phosphate import ATP-binding protein...”
- Eukaryotic-type serine/threonine protein kinase StkP is a global regulator of gene expression in Streptococcus pneumoniae
Sasková, Journal of bacteriology 2007 - “...Hypothetical genes spr0021 spr0053 spr0561 spr1324 spr1408 spr1935 spr1945 Hypothetical genes a spr1482 spr1623 spr1624 spr1625 spr1626 spr1768 The ratio of...”
- Transcriptional regulation and signature patterns revealed by microarray analyses of Streptococcus pneumoniae R6 challenged with sublethal concentrations of translation inhibitors
Ng, Journal of bacteriology 2003 - “...1.3 1.4 6.0 2.0 1.8 1.8 2.0 2.0 2.3 12.0 spr1935 2.5 2.2 1.8 1.8 Amino acid biosynthesis aspC ilvN ilvC metE metF ilvE asd dapA glyA metY-truncation...”
- Autoinducer 2 Signaling via the Phosphotransferase FruA Drives Galactose Utilization by Streptococcus pneumoniae, Resulting in Hypervirulence
Trappetti, mBio 2017 - “...SPD_1650 Iron compound ABC transporter 1.5 SPD_1834 adhE Bifunctional acetaldehyde-coenzyme A/alcohol dehydrogenase 2.0 0.1 0.1 SPD_1956 ilvD Dihydroxy-acid dehydratase 0.6 SPD_2012 glpO Alpha-glycerophosphate oxidase 1.7 SPD_2013 glpK Glycerol kinase 1.6 a Differential gene expression was determined by RNA-seq analysis as described in Materials and Methods. Data...”
- Sialic acid-mediated gene expression in Streptococcus pneumoniae and role of NanR as a transcriptional activator of the nan gene cluster
Afzal, Applied and environmental microbiology 2015 - “...spd_1098 spd_1099 spd_1158 spd_1514 spd_1515 spd_1516 spd_1956 Ratiob Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein ABC...”
- Characterization of central carbon metabolism of Streptococcus pneumoniae by isotopologue profiling
Härtel, The Journal of biological chemistry 2012 - “...(ilvC, SPD_0406 (EC 1.1.1.86)), dihydroxy-acid dehydratase (ilvD, SPD_1956 (EC 4.2.1.9)), and BCAA transaminase (ilvE, SPD_0749 (EC 2.6.1.42)) were identified...”
6ovtA / P9WKJ5 Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
Aligns to 19:562 / 562 (96.8%), covers 99.3% of TIGR00110, 711.5 bits
- Ligands: magnesium ion; fe2/s2 (inorganic) cluster (6ovtA)
ILVD_MYCTU / P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
Rv0189c dihydroxy-acid dehydratase from Mycobacterium tuberculosis H37Rv
NP_214703 dihydroxy-acid dehydratase from Mycobacterium tuberculosis H37Rv
Aligns to 32:575 / 575 (94.6%), covers 99.3% of TIGR00110, 711.4 bits
- function: Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo- 3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3- dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively. Is specific for the (R) isomer of 2,3-dihydroxy-3-methylbutanoate, with no catalytic activity against the (S) isomer.
catalytic activity: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O (RHEA:24809)
catalytic activity: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2- oxopentanoate + H2O (RHEA:27694)
cofactor: [2Fe-2S] cluster (Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.)
cofactor: Mg(2+)
subunit: Homodimer.
disruption phenotype: Cells lacking this gene display impaired growth. - Unraveling tuberculosis patient cluster transmission chains: integrating WGS-based network with clinical and epidemiological insights
Sadovska, Frontiers in public health 2024 - “...by isolates of the cases F4 (SIT254) and B2 (SIT42), respectively; p.Gly35Ser in the locus Rv0189c ( ilvD gene) was detected in J1 and J2 (SIT53) isolates, and synonymous variant g.221163G>T in the codon Val187 was found in case O1 Mtb isolates. No recently acquired variants...”
- The active site of the Mycobacterium tuberculosis branched-chain amino acid biosynthesis enzyme dihydroxyacid dehydratase contains a 2Fe-2S cluster
Bashiri, The Journal of biological chemistry 2019 (secret) - Novel T7 Phage Display Library Detects Classifiers for Active Mycobacterium Tuberculosis Infection
Talwar, Viruses 2018 - “...2614 2619 10.1128/JB.00224-12 22427625 34. Singh V. Chandra D. Srivastava B.S. Srivastava R. Downregulation of Rv0189c, encoding a dihydroxyacid dehydratase, affects growth of Mycobacterium tuberculosis in vitro and in mice Microbiology 2011 157 38 46 10.1099/mic.0.042358-0 20864475 35. Kolly G.S. Sala C. Vocat A. Cole S.T....”
- “...Rv2007c 1.36 10 9 7.27 10 7 0.92 0.91 0.89 P51_BP3_131 Dihydroxy acid dehydratase ilvD Rv0189c 2.15 10 8 3.84 10 6 0.95 0.92 0.98 P51_BP3_137 Transketolase TKT Rv1449c 7.14 10 8 9.72 10 6 0.95 1 0.81 P197_BP4_1078 Signal peptidase lepB Rv2903 1.44 10 7...”
- A systematic review of East African-Indian family of Mycobacterium tuberculosis in Brazil
Duarte, The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases 2017 - “...Rv0041 Rv0041_384a>G S 34 92199 Rv0083 Rv0083_188t>G S 34 157292 Rv0129c Rv0129c_309g>A S 33 220050 Rv0189c Rv0189c_1674g>A S 34 311613 Rv0260c Rv0260c_1047c>A S 34 720863 Rv0629c Rv0629c_870c>A S 13 797736 Rv0697 Rv0697_804c>T S 34 918316 Rv0824c Rv0824c_435a>G S 34 923065 Rv0831c Rv0831c_645a>T S 34 1047683 Rv0938...”
- SMRT genome assembly corrects reference errors, resolving the genetic basis of virulence in Mycobacterium tuberculosis
Elghraoui, BMC genomics 2017 - “...our Assembly Rv0037c Rv0037c Probable conserved integral membrane protein Rv0124 PE_PGRS2 PE-PGRS family protein PE_PGRS2 Rv0189c ilvD Probable dihydroxy-acid dehydratase IlvD (dad) [ 48 , 58 ] Rv0279c PE_PGRS4 PE-PGRS family protein PE_PGRS4 Rv0383c Rv0383c Possible conserved secreted protein masks sequencing error in H37Rv [ 8...”
- Bacterial Branched-Chain Amino Acid Biosynthesis: Structures, Mechanisms, and Drugability
Amorim, Biochemistry 2017 - “...262 274 4933736 83 Singh V Chandra D Srivastava BS Srivastava R 2011 Downregulation of Rv0189c, encoding a dihydroxyacid dehydratase, affects growth of Mycobacterium tuberculosis in vitro and in mice Microbiology 157 38 46 20864475 84 Kiritani K Narise S Wagner RP 1966 The dihydroxy acid...”
- “...isomeroreductase ilv C 1.1.1.86 4YPO, 1QMG, 1YRL, 1SR9 Rv3001c herbicides dihydroxyacid dehydratase ilv D 4.2.1.9 Rv0189c a isopropylmalate synthase leu A 2.3.3.13 3U6W, 3FIG, 4OV9 Rv3710 a isopropylmalate isomerase leu CD 4.2.1.33 3Q3W, 3H5E, 3H5H, 3H5J, 2HCU (C); 3H5H (D) Rv2988c (C), Rv2987c (D) a isopropylmalate...”
- Development of a T7 Phage Display Library to Detect Sarcoidosis and Tuberculosis by a Panel of Novel Antigens
Talwar, EBioMedicine 2015 - “...TKT 2.710 6 0.86 82 76 P51-BP4_563 (BAL) Dihydroxy acid dehydratase ( Mycobacterium tuberculosis ) Rv0189C 1.0410 6 0.85 76 86 Clone Decreased in TB vs sarcoidosis subjects P51_BP3_113 (BAL) Chain A Mycobacterium tuberculosis BfrA 1.210 10 0.9 88 85 P51_BP3_200 (BAL) Disabled homologue 2 isoform...”
- Equal opportunity for low-degree network nodes: a PageRank-based method for protein target identification in metabolic graphs
Bánky, PloS one 2013 - “...0.0030 Rv2607 pdxH R00277 0.0061 3 2 0.0030 Rv2607 pdxH R01209 0.0025 7 1 0.0025 Rv0189c ilvD R03051 0.0028 3 2 0.0014 Rv3001c ilvC R06905 0.0013 1 1 0.0013 bnsG R03968 0.0020 4 2 0.0010 Rv2987c(leuD) Rv2988c(leuC) R04942 0.0020 3 2 0.0010 Rv1077 cysM R04440 0.0020...”
- “...reported [33] that the downregulation of the third largest scoring protein with gene name ilvD (Rv0189c, a dihydroxyacid dehydratase) affects the growth of Mycobacterium tuberculosis in vitro and in mice. The sixth highest scoring hit, the leuD gene (Rv2987c) is shown to be essential in Mycobacterium...”
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- Downregulation of Rv0189c, encoding a dihydroxyacid dehydratase, affects growth of Mycobacterium tuberculosis in vitro and in mice.
Singh, Microbiology (Reading, England) 2011 (PubMed)- GeneRIF: It may be concluded that the DHAD encoded by Rv0189c is essential for the survival of Mycobacterium tuberculosis and could be a potential drug/vaccine target, as it is absent in mammals.
llmg_1280 dihydroxy-acid dehydratase from Lactococcus lactis subsp. cremoris MG1363
Aligns to 29:570 / 570 (95.1%), covers 99.6% of TIGR00110, 710.4 bits
- Transcriptional response of Lactococcus lactis during bacterial emulsification
Tarazanova, PloS one 2019 - “...1.2e-10 llmg_1298 hisC histidinol-phosphate aminotransferase 8.6 1.2e-10 llmg_1279 ilvB acetolactate synthase catalytic subunit 4.9 1.9e-7 llmg_1280 ilvD dihydroxy-acid dehydratase 4.9 3.6e-8 llmg_1183 gltB glutamate synthase. large subunit 4.3 3.4e-4 llmg_1290 hisF imidazole glycerol phosphate synthase subunit HisF 4.9 1.9e-4 llmg_1291 hisA 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase 4.3...”
MRA_0197 dihydroxy-acid dehydratase from Mycobacterium tuberculosis H37Ra
Aligns to 32:575 / 575 (94.6%), covers 99.3% of TIGR00110, 709.8 bits
- Genetic basis of virulence attenuation revealed by comparative genomic analysis of Mycobacterium tuberculosis strain H37Ra versus H37Rv
Zheng, PloS one 2008 - “...transmembrane protein MRA_0105 peptide synthetase SNV Rv0101 peptide syntherase Nrp MRA_0131 PE-PGRS SNV Rv0124 PE-PGRS MRA_0197 dihydroxy-acid dehydratase SNV Rv0189c dihydroxy-acid dehydratase MRA_0288 PE-PGRS family protein insertion+deletion+SNV Rv0279c PE-PGRS MRA_0391 up conserved secreted protein deletion Rv0383c up Conserved secreted protein MRA_0585 PE-PGRS SNV Rv0578c PE-PGRS MRA_0646...”
- “...this nrdHIEF2 operon might impact exit from dormancy or survival in vivo . The ilvD (MRA_0197, Rv0189c) gene encoding dihydroxy-acid dehydratase, an essential enzyme for branch chain amino acid and pantothenate (coenzyme A) biosynthesis, has a highly conserved Val284 (GTA) being substituted by Gly284 (GGA) in...”
MGL_3741 uncharacterized protein from Malassezia globosa CBS 7966
Aligns to 35:589 / 589 (94.2%), covers 99.6% of TIGR00110, 695.2 bits
- Production and Quantification of Virulence Factors in Malassezia Species
Hadrich, Polish journal of microbiology 2022 - “...of Aghaei Gharehbolagh et al. (2018), RT-PCR was used to investigate the contribution of the MGL_3741 gene to the pathogenicity of M. globosa in pityriasis versicolor. These authors revealed that this gene can be related to the yeasts pathogenicity and is a candidate for the new...”
- “...Hashemi SJ Daie Ghazvini R Asgari Y Agha Kuchak Afshari S Seyedmousavi S Rezaie S MGL_3741 gene contributes to pathogenicity of Malassezia globosa in pityriasis versicolor Mycoses 2018 Dec 61 12 938 944 10.1111/myc.12840 30106184 Angiolella L Leone C Rojas F Mussin J de Los Angeles...”
- Malassezia-Associated Skin Diseases, the Use of Diagnostics and Treatment
Saunte, Frontiers in cellular and infection microbiology 2020 - “...in lesions. But the reasons for hyphal growth are still unknown. The activation of the MGL_3741 gene which encodes the enzyme Dihydroxy acid dehydratase (DHAD) in M. globosa has been implicated as it is present in lesional but not non-lesional skin (Aghaei Gharehbolagh et al., 2018...”
- “...S. J. Daie Ghazvini R. Asgari Y. Agha Kuchak Afshari S. . ( 2018 ). MGL_3741 gene contributes to pathogenicity of Malassezia globosa in pityriasis versicolor . Mycoses 61 , 938 944 . 10.1111/myc.12840 30106184 Akaza N. Akamatsu H. Sasaki Y. Kishi M. Mizutani H. Sano...”
- MGL_3741 gene contributes to pathogenicity of Malassezia globosa in pityriasis versicolor
Aghaei, Mycoses 2018 (PubMed)- “...2018 DOI: 10.1111/myc.12840 ORIGINAL ARTICLE MGL_3741 gene contributes to pathogenicity of Malassezia globosa in pityriasis versicolor Sanaz Aghaei...”
- “...PCR to investigate the differentially expressed of the MGL_3741 gene in healthy and pathogenic states. Our results indicate a significant difference between two...”
PITG_20759 dihydroxy-acid dehydratase from Phytophthora infestans T30-4
Aligns to 84:561 / 561 (85.2%), covers 87.1% of TIGR00110, 689.8 bits
CNH01530 dihydroxy-acid dehydratase, putative from Cryptococcus neoformans var. neoformans JEC21
Aligns to 46:596 / 596 (92.4%), covers 99.6% of TIGR00110, 687.8 bits
- Comprehensive genome-scale metabolic model of the human pathogen Cryptococcus neoformans: A platform for understanding pathogen metabolism and identifying new drug targets
Tezcan, Frontiers in bioinformatics 2023 - “...Glycine, Serine and Threonine Metabolism CND03580 Amino Sugar and Nucleotide Sugar Metabolism CNA02570, CNN01460, CNF02480, CNH01530 CNH01520, CNA02270, CNH03010 Valine, Leucine and Isoleucine Biosynthesis and Degradation CND01200, CNK00580, CND03850, CNG00170, CND06290 Lysine Biosynthesis CNJ01640 Transport CNM00100 Terpenoid Backbone Biosynthesis CNB02610, CNH03390, CNN02320 Starch and Sucrose Metabolism...”
rrnAC0302 dihydroxy-acid dehydratase from Haloarcula marismortui ATCC 43049
Aligns to 29:570 / 575 (94.3%), covers 99.6% of TIGR00110, 677.9 bits
CHLNCDRAFT_58991 hypothetical protein from Chlorella variabilis
E1ZPZ3 dihydroxy-acid dehydratase from Chlorella variabilis
Aligns to 408:909 / 1102 (45.6%), covers 89.9% of TIGR00110, 674.2 bits
- Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae
Rai, Scientific reports 2017 - “...15. E1ZQQ9 Proteasome subunit alpha type CHLNCDRAFT_27583 2 0.8640.49 1.6760.58 2.0300.85 16. E1ZPZ3 Dihydroxy-acid dehydratase CHLNCDRAFT_58991 2 1.4190.57 2.1591.25 2.1170.78 Photosynthesis 17 E1Z6S6 Putative uncharacterized protein (photosystem II assembly) CHLNCDRAFT_140330 1 1.4510.19 2.3460.38 2.6840.55 18 E1ZBP9 FerredoxinNADP reductase CHLNCDRAFT_35035 3 1.3120.12 1.8530.19 2.1210.17 19 E1ZQR2 Putative...”
- Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae
Rai, Scientific reports 2017 - “...1.5320.02 1.6660.14 2.5990.33 15. E1ZQQ9 Proteasome subunit alpha type CHLNCDRAFT_27583 2 0.8640.49 1.6760.58 2.0300.85 16. E1ZPZ3 Dihydroxy-acid dehydratase CHLNCDRAFT_58991 2 1.4190.57 2.1591.25 2.1170.78 Photosynthesis 17 E1Z6S6 Putative uncharacterized protein (photosystem II assembly) CHLNCDRAFT_140330 1 1.4510.19 2.3460.38 2.6840.55 18 E1ZBP9 FerredoxinNADP reductase CHLNCDRAFT_35035 3 1.3120.12 1.8530.19 2.1210.17...”
- “...maintain the intracellular nitrogen homeostasis. 6 , 49 , 50 2 Amino acid biosynthesis 9 E1ZPZ3, E1Z4T9, E1ZF33, A0A087SJX6, A0A087SKJ2, E1ZEF2, E1ZIW5, E1Z357, E1ZP71 To maintain the overall intracellular levels of nitrogen, amino acid biosynthesis particularly of aspartate, glutamate and arginine is elevated. Accumulation of arginine...”
AO090009000414 No description from Aspergillus oryzae RIB40
Aligns to 51:607 / 608 (91.6%), covers 99.6% of TIGR00110, 661.1 bits
AFUA_1G03550 mitochondrial dihydroxy acid dehydratase, putative from Aspergillus fumigatus Af293
Aligns to 1:541 / 542 (99.8%), covers 96.9% of TIGR00110, 660.6 bits
- The Aspergillus fumigatus dihydroxyacid dehydratase Ilv3A/IlvC is required for full virulence
Oliver, PloS one 2012 - “...fungus Aspergillus fumigatus by homology to Saccharomyces cerevisiae ILV3. Two of these genes, AFUA_2G14210 and AFUA_1G03550, initially designated AfIlv3A and AfIlv3B for this study, clustered in the same group as S. cerevisiae ILV3 following phylogenetic analysis. To investigate the functions of these genes, AfIlv3A and AfIlv3B...”
- “...leading to the identification of four A. fumigatus proteins. Proteins encoded by the genes AFUA_2G14210, AFUA_1G03550, AFUA_1G07330 and AFUA_2G16300 were 63%, 55%, 31% and 29% identical to S. cerevisiae Ilv3p, respectively. For the purposes of clarity they shall be referred to as AfIlv3A (AFUA_2G14210), AfIlv3B (AFUA_1G03550),...”
CC77DRAFT_1063831 dihydroxy-acid dehydratase-like protein from Alternaria alternata
Aligns to 51:604 / 605 (91.6%), covers 99.6% of TIGR00110, 655.5 bits
- Combination of Bacillus tequilensis with difenoconazole to control pear black spot and the related synergistic mechanism
Bi, Frontiers in microbiology 2024 - “...CACACACGTTCACCACTCAG CAGAGTCAGCAGATTGTCGC CC77DRAFT_942416 (down) ATP-dependent RNA helicase TCATTTTCGTCAGGACCCGA TTGATGACCATGGTGACGGA CC77DRAFT_938423 (down) ADP-ribose pyrophosphatase CTCCTCGGATGCAAAATGGG ACGGGTGGTCTGAATTGGAT CC77DRAFT_1063831 (down) Valine, leucine, and isoleucine biosynthesis CACTGTGAGTAGACGCATGC ACCAGCATACCTTCTCCACC CC77DRAFT_1061793(down) Glycine, serine, and threonine metabolism TGGAAGCTGCTGGATCTCAA TTCACCCTTTGCGACCTTTG 2.4.2 Verification of the main metabolic components involved in the synergistic effect 2.4.2.1 Detection...”
NCU05683 dihydroxy-acid dehydratase from Neurospora crassa OR74A
Aligns to 70:635 / 640 (88.4%), covers 99.6% of TIGR00110, 641.7 bits
- Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa
Lan, Frontiers in microbiology 2021 - “...and fsd-1 , and several genes with unknown functions, such as NCU07743 , NCU09716 , NCU05683 , and NCU05789 , were also selected for RT-qPCR analysis. In wild type, the transcript levels of these genes were dramatically increased during protoperithecium formation, but these increases were abolished...”
- “...continuous light for 7 days. However, the mutant phenotype of NCU09716, NCU05104, NCU01383, NCU03530, NCU05789, NCU05683, NCU08131, and NCU07180 did not co-segregate well with hygromycin resistance ( Chinnici et al., 2014 ), indicating that additional genetic modifications are likely the cause of the observed deficiencies. Therefore,...”
bll6092 dihydroxy-acid dehydratase from Bradyrhizobium japonicum USDA 110
Aligns to 17:555 / 564 (95.6%), covers 99.8% of TIGR00110, 626.0 bits
Or search for genetic data about TIGR00110 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory