PaperBLAST
PaperBLAST Hits for 73 a.a. (MTVSIQMAGN...)
Show query sequence
>73 a.a. (MTVSIQMAGN...)
MTVSIQMAGNLWKVHVKAGDQIEKGQEVAILESMKMEIPIVADRSGIVKEVKKKEGDFVN
EGDVLLELSNSTQ
Running BLASTp...
Found 255 similar proteins in the literature:
BLAP_BACSU / C0H419 Biotin/lipoyl attachment protein; BLAP from Bacillus subtilis (strain 168) (see paper)
100% identity, 100% coverage
2b8gA / C0H419 Solution structure of bacillus subtilis blap biotinylated-form (energy minimized mean structure)
100% identity, 99% coverage
GBAA_2549 acetyl-CoA carboxylase biotin carboxyl carrier protein subunit from Bacillus anthracis str. 'Ames Ancestor'
B7HS68 Lipoyl-binding domain-containing protein from Bacillus cereus (strain AH187)
61% identity, 90% coverage
- AtxA-Controlled Small RNAs of Bacillus anthracis Virulence Plasmid pXO1 Regulate Gene Expression in trans
Corsi, Frontiers in microbiology 2020 - “...0.002 U-CUUUAUUCCCUc 182 170 inha1 AgGAAAUAAGGG Au 12 +2 11.54 0.01 XrrB AUGGUaCCCCUUUg 194 180 GBAA_2549 UACUAaGGGGGAAa 19 6 13.7 0.002 a Complementarity between sequences is shown in uppercase letters. Dashes indicate a break in the complementarity region. b Indicates start and end of complementarity region...”
- “...mutant by a 4.0 fold-change. Instead, we found that XrrB is predicted to interact with GBAA_2549, at the 5 UTR of the transcript ( Table 4 ). Interestingly, expression of this transcript increases 10-fold in the xrrA xrrB mutant, and only 3.5- and 2.0-fold in the...”
- Bacillus cereus Decreases NHE and CLO Exotoxin Synthesis to Maintain Appropriate Proteome Dynamics During Growth at Low Temperature
Duport, Toxins 2020 - “...AcpP and increased for Hpr and B7HXP4, and to a lesser extent for B7HUU3 and B7HS68 ( Figure 4 ). Interestingly, AcpP is a predicted acyl carrier protein linked to fatty acid synthesis regulation in response to cold stress [ 38 ], and CspD is a...”
- “...protein that may contribute to Fe-S cluster assembly in proteins, mainly at low temperatures; and B7HS68 is a predicted component of pathways controlling lipid transport and metabolism. (iii) Group C was distinguished from the other groups by a high log 2 FC in S-phase samples. With...”
VV1_1601 oxaloacetate decarboxylase from Vibrio vulnificus CMCP6
52% identity, 11% coverage
Q6A1F6 oxaloacetate decarboxylase (EC 4.1.1.112) from Vibrio cholerae (see paper)
TC 3.B.1.1.6 / Q6A1F6 Oxaloacetate decarboxylase, component of Na+-exporting oxaloacetate decarboxylase with three subunits, OadA (α), OadB (β) and OadG (γ) of 599 aas and 0 TMSs, 433 aas and 11 TMSs, and 90 aas and 1 TMS, respectively from Vibrio cholerae
43% identity, 11% coverage
- substrates: Na+
tcdb comment: These three subunits are 72%, 70% and 40% identical to 3.B.1.1.1. The stoichiometry of the three subunits is 4:2:2, and large amounts of the protein can be generated for crystalographic studies (Inoue and Li 2015)
Q87XD5 Urea amidolyase-related protein from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
PSPTO_4243 urea amidolyase-related protein from Pseudomonas syringae pv. tomato str. DC3000
45% identity, 5% coverage
TK1624 methylmalonyl-CoA decarboxylase, gamma subunit from Thermococcus kodakaraensis KOD1
48% identity, 42% coverage
Smar_0341 oxaloacetate decarboxylase alpha subunit from Staphylothermus marinus F1
49% identity, 11% coverage
PBPRA2298 putative oxaloacetate decarboxylase, alpha subunit from Photobacterium profundum SS9
46% identity, 11% coverage
SPO2542 acetyl-CoA carboxylase biotin carboxyl carrier protein subunit from Ruegeria pomeroyi DSS-3
43% identity, 93% coverage
TK0990 oxaloacetate decarboxylase, alpha subunit from Thermococcus kodakaraensis KOD1
47% identity, 11% coverage
MMDC_PROMO / O54030 Methylmalonyl-CoA decarboxylase subunit gamma; EC 7.2.4.3 from Propionigenium modestum (see paper)
mmdC / CAA05139.1 methylmalonyl-CoA decarboxylase, gamma-subunit from Propionigenium modestum (see paper)
44% identity, 51% coverage
- function: Biotin-containing subunit of the sodium ion pump methylmalonyl-CoA decarboxylase, which converts the chemical energy of a decarboxylation reaction into an electrochemical gradient of Na(+) ions across the cytoplasmic membrane, thereby creating a sodium ion motive force that is used for ATP synthesis.
catalytic activity: (S)-methylmalonyl-CoA + Na(+)(in) + H(+)(out) = propanoyl-CoA + Na(+)(out) + CO2 (RHEA:21396)
cofactor: biotin
subunit: The methylmalonyl-CoA decarboxylase is composed of four subunits: the carboxyltransferase alpha subunit (MmdA), the tunnel beta subunit (MmdB), the biotin-containing gamma subunit (MmdC) and the delta subunit (MmdD).
FRAAL6005 Biotin carboxyl carrier protein from Frankia alni ACN14a
43% identity, 83% coverage
- The PEG-responding desiccome of the alder microsymbiont Frankia alni
Ghedira, Scientific reports 2018 - “...FRAAL4147 Cytochrome c oxidase polypeptide I (AA3 subunit 1) CtaD 3.70 FRAAL5625 Poly(3-hydroxybutyrate) depolymerasePha 2.83 FRAAL6005 Biotin carboxyl carrier protein 2.00 FRAAL6082 Succinate-semialdehyde dehydrogenase I, NADP-dependent 2.00 FRAAL5481 Malate synthase G AceB 1.75 FRAAL0315 Aldehyde dehydrogenase 1.71 FRAAL1829 [NiFe] uptake hydrogenase, large subunit HupL 1.70 FRAAL1039...”
G7YW97 Propionyl-CoA carboxylase alpha chain from Clonorchis sinensis
47% identity, 8% coverage
CJJ81176_0940 oxaloacetate decarboxylase, alpha subunit, putative from Campylobacter jejuni subsp. jejuni 81-176
44% identity, 11% coverage
PN96_01210 sodium-extruding oxaloacetate decarboxylase subunit alpha from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759
46% identity, 11% coverage
WU75_16665 sodium-extruding oxaloacetate decarboxylase subunit alpha from Vibrio parahaemolyticus
46% identity, 11% coverage
PM1422 unknown from Pasteurella multocida subsp. multocida str. Pm70
46% identity, 11% coverage
- Using genomics to understand inter- and intra- outbreak diversity of Pasteurella multocida isolates associated with fowl cholera in meat chickens
Omaleki, Microbial genomics 2020 - “...PM1330 to PM1358, PM1766*, PM1767, PM1768 29 5 20 L3 gatG 14bp deletion August 2010 PM1422 to PM1430 9 6 20 L3 gatG 14bp deletion June 2012 PM1614, PM1615 2 na 20 L1 None July 2012 PM1634 to PM1651 17(19) 4 20 L1 None August 2013...”
- “...the LPS from isolate PM1439 as well as one of the strains from this farm (PM1422) has previously revealed their outer core finishes at the first galactose and consists of Hep.[Glu].Glu.Gal [ 12, 18 ]. This confirms the role of the 14bp deletion in gatG ,...”
- The Myriad Properties of Pasteurella multocida Lipopolysaccharide
Harper, Toxins 2017 - “...challenge strain (L3 P1059 gatG mutant), gave solid protection against challenge with the L3 strain PM1422 (83%, p = 0.006; Figure 4 ). Vaccination with a bacterin containing the P1059 natB mutant, which produces an L3 LPS structure with one extra Gal compared to the PM1422...”
- “...weeks after the booster vaccination, either with the wild type VP161 (L1) strain, or the PM1422 (L3) strain. The vaccine strains and corresponding LPS structures (grey box) are shown at the left. The percent survival following live vaccination is shown in the green box, and percent...”
Q9KUH1 oxaloacetate decarboxylase (EC 4.1.1.112) from Vibrio cholerae (see paper)
VC0550 oxaloacetate decarboxylase, alpha subunit from Vibrio cholerae O1 biovar eltor str. N16961
43% identity, 11% coverage
Cj0933c putative pyruvate carboxylase B subunit from Campylobacter jejuni subsp. jejuni NCTC 11168
46% identity, 10% coverage
- Proteome profiling of Campylobacter jejuni 81-176 at 37 °C and 42 °C by label-free mass spectrometry
Dreyer, BMC microbiology 2024 - “...detected as down-regulated at 37C in both analyses. To note, putative pyruvate carboxylase subunit B (Cj0933c, PaxDb: Q0P9W6), periplasmic protein p19 (Cj1659, PaxDb: Q0P7X0), bacterial non-heme ferritin (Cft, PaxDb: Q46106), two-component regulator (Cj0355c, PaxDb: Q0PBF4), putative periplasmic protein (Cj0561c, PaxDb: Q0PAV5), putative periplasmic protein (Cj1380, PaxDb:...”
- Genome-wide insights into population structure and host specificity of Campylobacter jejuni
Epping, Scientific reports 2021 - “...preference d Accessory/variant e Pig Cattle Chicken Host generalists Other n n n n n Cj0933c pyc B Putative pyruvate carboxylase B subunit 882.094 C Energy production and conversion 26 56 90 255 63 V Cj0478 rpo B DNA-directed RNA polymerase beta chain 444.215 K Transcription...”
- The Novel Protein Cj0371 Inhibits Chemotaxis of Campylobacter jejuni
Du, Frontiers in microbiology 2018 - “...41929 1 cj1487c Oxidative phosphorylation 31162 1 cj1037c ATP binding pyruvate carboxylase activity 54413 2 cj0933c Ribose-phosphate pyrophosphokinase 65834 8 Bacterial chemotaxis cj0285c ( cheV ) Chemotaxis protein 35858 9 cj0284c ( cheA ) Chemotaxis protein 85300 3 cj0019c Methyl-accepting chemotaxis protein 66845 1 cj1564 Methyl-accepting...”
- Characterisation of Aerotolerant Forms of a Robust Chicken Colonizing Campylobacter coli
O'Kane, Frontiers in microbiology 2017 - “...Therefore, this protein is likely to be the ortholog of PycB, encoded by the gene cj0933c in C. jejuni NCTC 11168. Pyruvate carboxylase in C. jejuni is composed of two subunits, PycA and PycB ( Velayudhan and Kelly, 2002 ). These correspond with the biotin carboxylase...”
- Defining the metabolic requirements for the growth and colonization capacity of Campylobacter jejuni
Hofreuter, Frontiers in cellular and infection microbiology 2014 - “...carboxykinase PckA (Cj0932c), the pyruvate kinase Pyk (Cj0392c) and the pyruvate carboxylase (PycA, Cj1037c; PycB, Cj0933c) comprise a crucial metabolic junction between catabolism and anabolism in C. jejuni (Velayudhan and Kelly, 2002 ). These enzymes are required for the oxaloacetatePEPpyruvate conversion that comprises a metabolic triangle,...”
PCCA_HALMT / I3R7G3 Propionyl-CoA carboxylase, biotin carboxylase and biotin-carboxyl carrier subunit; PCC; EC 6.4.1.3; EC 6.3.4.14 from Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) (see paper)
I3R7G3 propionyl-CoA carboxylase (subunit 3/3) (EC 6.4.1.3) from Haloferax mediterranei (see paper)
49% identity, 11% coverage
- function: This is one of the subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), the enzyme catalyzing the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl- CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase is involved in propionate utilization and in the production of the poly(3-hydroxybutyrate-co-3- hydroxyvalerate)(PHBV), which is a water-insoluble biopolymer used as intracellular energy reserve material when cells grow under conditions of nutrient limitation (PubMed:25398867). Propionyl-CoA carboxylase is also able to catalyze the carboxylation of acetyl-CoA (PubMed:25398867).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) (RHEA:13501)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The propionyl coenzyme A carboxylase (PCC) complex is composed of three subunits: PccA (biotin carboxylase and biotin-carboxyl carrier), PccB (carboxyltransferase) and PccX.
B2RDE0 Propionyl-CoA carboxylase alpha chain, mitochondrial from Homo sapiens
45% identity, 9% coverage
PCCA / P05165 propionyl-CoA carboxylase α chain, mitochondrial (EC 6.4.1.3) from Homo sapiens (see 4 papers)
PCCA_HUMAN / P05165 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Homo sapiens (Human) (see 11 papers)
P05165 propionyl-CoA carboxylase (subunit 1/2) (EC 6.4.1.3) from Homo sapiens (see paper)
NP_000273 propionyl-CoA carboxylase alpha chain, mitochondrial isoform a precursor from Homo sapiens
45% identity, 9% coverage
- function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed:6765947, PubMed:8434582). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl- CoA/(S)-methylmalonyl-CoA (PubMed:10101253, PubMed:6765947, PubMed:8434582). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)- ethylmalonyl-CoA at a much lower rate (PubMed:6765947). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+) (RHEA:59520)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits (PubMed:20725044, PubMed:6765947). Interacts (via the biotin carboxylation domain) with SIRT4 (PubMed:23438705). Interacts with SIRT3 and SIRT5 (PubMed:23438705). - Raphe and ventrolateral medulla proteomics in sudden unexplained death in childhood with febrile seizure history
Leitner, Acta neuropathologica 2024 - “...1.3 RPL12 60S ribosomal protein L12 P30050 4.07E04 1.4 PCCA Propionyl-CoA carboxylase alpha chain, mitochondrial P05165 5.33E04 1.2 VAMP2 Vesicle-associated membrane protein 2 P63027 6.15E04 1.7 CCT3 T-complex protein 1 subunit gamma P49368 7.86E04 1.1 ATP6V1D V-type proton ATPase subunit D Q9Y5K8 8.20E04 1.2 FARSA PhenylalaninetRNA...”
- Generation and Characterization of Trastuzumab/Pertuzumab-Resistant HER2-Positive Breast Cancer Cell Lines.
Sanz-Álvarez, International journal of molecular sciences 2023 - “...0.32 1.65 10 28 426 P18564 ITGB6 Integrin beta 6 0.33 1.59 3 5 103 P05165 PCCA Propionyl-CoA carboxylase alpha chain, mitochondrial 0.34 1.57 7 10 144 Q8IW45 NAXD ATP-dependent (S)-NAD(P)H-hydrate dehydratase 0.37 1.44 5 10 163 Q96AY3 FKBP10 Peptidyl-prolyl cis/trans isomerase FKBP10 0.38 1.41 14...”
- Interactome Analysis of the ER Stress Sensor Perk Uncovers Key Components of ER-Mitochondria Contact Sites and Ca2+ Signalling
Sassano, Contact (Thousand Oaks (Ventura County, Calif.)) 2021 - “...94 31 100% 100% Propionyl-CoA carboxylase alpha chain, mitochondrial OS=Homo sapiens OX=9606 GN=PCCA PE=1 SV=4 P05165 PCCA 80 kDa 117 49 114 45 100% 100% Pyruvate carboxylase, mitochondrial OS=Homo sapiens OX=9606 GN=PC PE=1 SV=2 P11498 PC 130 kDa 122 54 121 49 100% 100% Considering our...”
- Proteome biology of primary colorectal carcinoma and corresponding liver metastases.
Fahrner, Neoplasia (New York, N.Y.) 2021 - “...0.917 2.35E-04 7.92E-03 PLG Plasminogen O95671 1.631 1.89E-06 4.55E-04 ASMTL Probable bifunctional dTTP/UTP pyrophosphatase/methyltransferase protein P05165 0.69 2.54E-03 3.95E-02 PCCA Propionyl-CoA carboxylase alpha chain P00734 0.896 2.93E-03 4.29E-02 F2 Prothrombin P11498 2.044 7.18E-07 2.77E-04 PC Pyruvate carboxylase Q99575 0.746 4.31E-04 1.14E-02 POP1 Ribonucleases P/MRP protein subunit...”
- Cigarette smoke extract stimulates bronchial epithelial cells to undergo a SUMOylation turnover.
Zhou, BMC pulmonary medicine 2020 - “...Down NACA P15104 Glutamine synthetase 0.336 Down GLUL P20290 Transcription factor BTF3 0.337 Down BTF3 P05165 Propionyl-CoA carboxylase alpha chain, mitochondrial 0.345 Down PCCA P08865 40S ribosomal protein SA 0.407 Down RPSA P16401 Histone H1.5 0.407 Down HIST1H1B P67809 Nuclease-sensitive element-binding protein 1 0.426 Down YBX1...”
- Investigating the structural impacts of a novel missense variant identified with whole exome sequencing in an Egyptian patient with propionic acidemia.
Ibrahim, Molecular genetics and metabolism reports 2020 - “...The protein sequence for the PCCA was obtained from the UniProt database with the ID P05165 [ 11 ]. The PCCA protein lacks the crystal structure in the public databases to date. Henceforth, the protein sequence obtained from the UniProt database was used to build the...”
- SAMHD1 Posttranscriptionally Controls the Expression of Foxp3 and Helios in Human T Regulatory Cells.
Kim, Journal of immunology (Baltimore, Md. : 1950) 2018 - “...MCCA_HUMAN 249.9351293 (Q96RQ3) Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Mitochondrial enzymes with Biotin cofactor PCCA_HUMAN 93.580363 (P05165) Propionyl-CoA carboxylase alpha chain, mitochondrial Mitochondrial enzymes with Biotin cofactor DLDH_HUMAN 165.104467 (P09622) Dihydrolipoyldehydrogenase, mitochondrial Photo damage-related proteins ARGI1_HUMAN 349.6449536 (P05089) Arginase-1 Photo damage-related proteins CASPE_HUMAN 198.6111486 (P31944) Caspase-14 Heterogeneous...”
- Combining patient proteomics and in vitro cardiomyocyte phenotype testing to identify potential mediators of heart failure with preserved ejection fraction
Raphael, Journal of translational medicine 2016 - “...YP010 Q96M66 Putative uncharacterized protein FLJ32790 PSMD4 P55036 26S proteasome non-ATPase regulatory subunit 4 PCCA P05165 Propionyl-CoA carboxylase alpha chain, mitochondrial TCPR2 O15040 Tectonin beta-propeller repeat-containing protein KPRP Q5T749 Keratinocyte proline-rich protein GTPB5 Q9H4K7 GTP-binding protein 5 CV031 O95567 Uncharacterized protein C22orf31 TFB2M Q9H5Q4 Dimethyladenosine transferase...”
- More
- The mitochondrial carboxylase PCCA interacts with Listeria monocytogenes phospholipase PlcB to modulate bacterial survival.
Wang, Applied and environmental microbiology 2024 - GeneRIF: The mitochondrial carboxylase PCCA interacts with Listeria monocytogenes phospholipase PlcB to modulate bacterial survival.
- A novel small molecule approach for the treatment of propionic and methylmalonic acidemias.
Armstrong, Molecular genetics and metabolism 2021 - GeneRIF: A novel small molecule approach for the treatment of propionic and methylmalonic acidemias.
- Functional Analysis of the PCCA and PCCB Gene Variants Predicted to Affect Splicing.
Bychkov, International journal of molecular sciences 2021 - GeneRIF: Functional Analysis of the PCCA and PCCB Gene Variants Predicted to Affect Splicing.
- Identification of 34 novel mutations in propionic acidemia: Functional characterization of missense variants and phenotype associations.
Rivera-Barahona, Molecular genetics and metabolism 2018 (PubMed)- GeneRIF: his work represents a large-scale update on pathogenic mutations in the PCCA and PCCB genes causing Propionic acidemia (PA), and confirms previous reports indicating a major causative role of mutation-induced protein destabilization
- Seventeen Novel Mutations in PCCA and PCCB Genes in Indian Propionic Acidemia Patients, and Their Outcomes.
Gupta, Genetic testing and molecular biomarkers 2016 (PubMed)- GeneRIF: The majority of patients had mutations in the PCCA gene (18/25). A total of 26 mutations were noted: 20 in the PCCA gene and 6 in PCCB gene. Seventeen mutations were novel (14 in PCCA and 3 in PCCB). The SNP c.937C>T (p.Arg313Ter), was noted in 9/36 (25%) alleles in the PCCA gene
- Frameshift mutations of OGDH, PPAT and PCCA genes in gastric and colorectal cancers.
Jo, Neoplasma 2016 (PubMed)- GeneRIF: The data indicate that amino acid/nucleotide metabolism-related genes OGDH, PPAT and PCCA acquire somatic mutations in microsatellite instability-high gastric cancers and colorectal cancers and that mutational intratumoral heterogeneity may occur in at least some of these tumors.
- [Analysis of PCCA and PCCB gene mutations in patients with propionic acidemia].
Chen, Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 2015 (PubMed)- GeneRIF: Ten propionic acidemia mutations were confirmed, including 8 affecting the PCCA gene and 2 affecting the PCCB gene
- Clinical characteristics and mutation analysis of propionic acidemia in Thailand.
Vatanavicharn, World journal of pediatrics : WJP 2014 (PubMed)- GeneRIF: Mutation analysis of the PCCA gene identified homozygous c.1284+1G>A in patient 1, c.230G>A (p.R77Q) and c.1855C>T (p.R619X) in patient 2, homozygous c.2125T>C (p.S709P) in patient 3, and only one mutant allele, c.231+1G>T in patient 4.
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7ybuA / P05165 Human propionyl-coenzyme a carboxylase (see paper)
45% identity, 9% coverage
- Ligand: 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal (7ybuA)
PCCA_PIG / P0DTA4 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Sus scrofa (Pig) (see paper)
47% identity, 8% coverage
- function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed:13752080). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl- CoA/(S)-methylmalonyl-CoA (PubMed:13752080). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then tranfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate (PubMed:13752080). Other alternative minor substrates include (2E)- butenoyl-CoA/crotonoyl-CoA (PubMed:13752080).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+) (RHEA:59520)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits. Interacts (via the biotin carboxylation domain) with SIRT4. Interacts with SIRT3 and SIRT5. - Label-Free Quantitative Analysis of Pig Liver Proteome after Hepatitis E Virus Infection
Martino, Viruses 2024 - “...phosphoenolpyruvate carboxykinase, cytosolic [GTP] 18 98.33 0.0036 2.90 P26234 VCL Vinculin 18 63.18 0.0007 2.28 P0DTA4 PCCA propionyl-CoA carboxylase alpha chain, mitochondrial 18 78.68 0.0025 1.98 A0A8D1MNH9 SPTAN1 spectrin alpha chain, non-erythrocytic 1 17 65.06 0.0000 2.68 P33198 IDH2 isocitrate dehydrogenase, mitochondrial 16 66.34 0.0113 1.53...”
A0A8I3S647 Propionyl-CoA carboxylase alpha chain, mitochondrial from Canis lupus familiaris
45% identity, 8% coverage
gcdC / Q9ZAA7 GcdC (EC 7.2.4.5) from Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / CCUG 9996 / CIP 106432 / VR4) (see 2 papers)
Q9ZAA7 glutaconyl-CoA decarboxylase (subunit 2/4) (EC 7.2.4.5) from Acidaminococcus fermentans (see 2 papers)
TC 3.B.1.1.3 / Q9ZAA7 GcdC, component of Na+-transporting glutaconyl-CoA decarboxylase from Acidaminococcus fermentans (see paper)
Acfer_1835 biotin/lipoyl attachment domain-containing protein from Acidaminococcus fermentans DSM 20731
46% identity, 47% coverage
- substrates: Na+
- Complete genome sequence of Acidaminococcus fermentans type strain (VR4)
Chang, Standards in genomic sciences 2010 - “...Acfer_1819 and 1818). The glutaconyl-CoA decarboxylase , and -subunits: gcdB , gcdC and gcdD (Acfer_1834, Acfer_1835 and Acfer_1836) are encoded nearly 15kb upstream from this operon, forming a second operon [ 43 ]. In addition to the above-mentioned protein complexes, the gene encoding glutamate dehydrogenase (NAD(P)(+))...”
- “...(subunit ) Acfer_1816 2-hydroxyglutaryl-CoA dehydratase activator protein Acfer_1817 glutaconyl-CoA decarboxylase subunit Acfer_1834 glutaconyl-CoA decarboxylase subunit Acfer_1835 glutaconyl-CoA decarboxylase subunit r Acfer_1836 glutaconyl-CoA decarboxylase sodium pump, subunit r Acfer_1477 acyl-CoA dehydrogenase domain protein Acfer_1575 acyl-CoA dehydrogenase domain protein Acfer_1583 acyl-CoA dehydrogenase domain protein Enzymes of -lactamase and...”
HD73_4231 pyruvate carboxylase from Bacillus thuringiensis serovar kurstaki str. HD73
44% identity, 6% coverage
- Signal Peptidase-Mediated Cleavage of the Anti-σ Factor RsiP at Site 1 Controls σP Activation and β-Lactam Resistance in Bacillus thuringiensis
Nauta, mBio 2021 - “...was added. Immunoblotting was performed using anti-GFP antisera. Streptavidin IR680LT was used to detect PycA (HD73_4231) and AccB (HD73_4487), which served as a loading control ( 68 , 69 ). The color blot with both anti-GFP and streptavidin on a single gel is shown in Fig.S2A...”
- “...was added. Immunoblotting was performed using anti-GFP antisera. Streptavidin IR680LT was used to detect PycA (HD73_4231) and AccB (HD73_4487), which served as loading controls ( 68 , 69 ). Numbers at the left indicate the molecular masses (in kilodaltons) of the ladder. The bands corresponding to...”
- The Penicillin-Binding Protein PbpP Is a Sensor of β-Lactams and Is Required for Activation of the Extracytoplasmic Function σ Factor σP in Bacillus thuringiensis
Nauta, mBio 2021 - “...immunoblotting was performed using anti-PbpP antisera and Bocillin-FL, and streptavidin IR680LT was used to detect HD73_4231 (PycA homolog), which served as a loading control ( 51 , 52 ). FigureS3A in the supplemental material is the color blot showing anti-PbpP antisera, Bocillin-FL, and streptavidin in a...”
- “...described in Materials and Methods using antisera against PbpP. Streptavidin IR680LT was used to detect HD73_4231 (PycA homolog), which served as a loading control ( 51 , 52 ). (Top) Color blot showing anti-PbpP, Bocillin, and streptavidin on a single image. (Middle) Color blot showing anti-PbpP...”
- Activation of the Extracytoplasmic Function σ Factor σP by β-Lactams in Bacillus thuringiensis Requires the Site-2 Protease RasP
Ho, mSphere 2019 - “...was probed with antisera against RsiP (-RsiP 76275 ). Streptavidin IR680LT was used to detect HD73_4231 (PycA homolog), which served as a loading control ( 62 , 63 ). The color blot showing both anti-RsiP and streptavidin on a single gel is shown in Fig.S3 ....”
- “...and GFP is wild type with pAH13 (UM20). Streptavidin IR680LT (red) was used to detect HD73_4231 (PycA homolog), which served as a loading control ( 62 , 63 ). Download FIGS3, TIF file, 1.5 MB . Copyright 2019 Ho et al. 2019 Ho et al. This...”
BC3947 Pyruvate carboxylase from Bacillus cereus ATCC 14579
Q819M9 Pyruvate carboxylase from Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711)
44% identity, 6% coverage
SCA02S_RS02525 biotin/lipoyl-containing protein from Sulfurospirillum cavolei NBRC 109482
47% identity, 10% coverage
PAP_03770 pyruvate/oxaloacetate carboxyltransferase from Palaeococcus pacificus DY20341
41% identity, 12% coverage
PA5435 oxaloacetate decarboxylase from Pseudomonas aeruginosa PAO1
NP_254122 pyruvate carboxylase subunit B from Pseudomonas aeruginosa PAO1
45% identity, 11% coverage
- Distinct transcriptome and traits of freshly dispersed <i>Pseudomonas aeruginosa</i> cells
Kalia, mSphere 2024 - “...0.47 0.803 0.55 PA5015 Pyruvate dehydrogenase 0.62 0.87 0.305 PA5016 Dihydrolipoamide acetyltransferase 0.346 0.644 0.117 PA5435 Probable transcarboxylase subunit 0.088 0.21 1.44 PA5436 Probable biotin carboxylase subunit of a transcarboxylase 0.42 0.46 1.58 Pentose phosphate pathway PA0330 Ribose 5-phosphate isomerase 0.77 0.28 0.302 PA0548 Transketolase 0.96...”
- The opportunistic pathogen Pseudomonas aeruginosa exploits bacterial biotin synthesis pathway to benefit its infectivity
Shi, PLoS pathogens 2023 - “...of P . aeruginosa reveals five putative biotinylated enzymes, namely PA1400, PA2012, PA2891, PA4847, and PA5435 ( S1 Table ). D. Streptavidin blot analyses for crude extracts of E . coli and P . aeruginosa . Unlike E . coli displaying a well-known AccB subunit with...”
- “...methyl crotonyl-CoA carboxylase), PA2891 (-subunit of geranyl-CoA carboxylase), PA4847 (AccB, biotin carboxyl carrier protein), and PA5435 (the probable transcarboxylase subunit) ( S1 Table ). To verify the bioinformatic output, we carried out streptavidin-based blot using bacterial crude extract. As expected, only one band of protein biotinylation...”
- Systems-wide dissection of organic acid assimilation in Pseudomonas aeruginosa reveals a novel path to underground metabolism
Dolan, 2022 - Systems-Wide Dissection of Organic Acid Assimilation in Pseudomonas aeruginosa Reveals a Novel Path To Underground Metabolism
Dolan, mBio 2022 - “...was downregulated (4.7 FC) during growth on propionate. By contrast, the pyruvate carboxylase-encoding genes pycA (PA5435) and pycB (PA5436), which catalyze the ATP-dependent carboxylation of pyruvate to yield oxaloacetate (OAA), were upregulated (2.6 FC) as was the regulator PycR ( 21 , 22 ). These alterations...”
- Full Transcriptomic Response of Pseudomonas aeruginosa to an Inulin-Derived Fructooligosaccharide
Rubio-Gómez, Frontiers in microbiology 2020 - “...ABC transporter 0.9 0.005 1.2 0.000 PA5369 pstS Phosphate ABC transporter 0.8 0.000 0.5 0.006 PA5435 oadA Probable transcarboxylase activity 0.6 0.002 0.9 0.000 The above table shows the list of the significant up regulated genes and control with a log2 fold change cut off 0.5...”
- “...0.001 PA5367 pstA Phosphate ABC transporter 0.7 0.001 PA5369 pstS Phosphate ABC transporter 0.7 0.000 PA5435 oadA Probable transcarboxylase activity 0.9 0.000 The above table shows the list of the significant up regulated genes with a log2 fold change cut off 0.5 by taking significant p-...”
- Traditional Chinese Medicine Tanreqing Inhibits Quorum Sensing Systems in Pseudomonas aeruginosa
Yang, Frontiers in microbiology 2020 - “...PA3582 glpK 1.5 2.2 Glycerol kinase PA4100 1.3 Probable dehydrogenase PA4956 rhdA 1.3 Thiosulfate:cyanide sulfurtransferase PA5435 2.3 1.3 Probable transcarboxylase subunit PA5436 2.7 1.1 Probable biotin carboxylase subunit of a transcarboxylase PA4442 cysN 1.7 1.9 + ATP sulfurylase GTP-binding subunit/APS kinase PA4443 cysD 2.1 1.1 +...”
- Bacterial Metabolism During Biofilm Growth Investigated by 13C Tracing
Wan, Frontiers in microbiology 2018 - “...data of qPCR experiments. The target samples were seven selected genes (PA4732, PA5110, PA3131, PA5192, PA5435, PA1580, and PA2828) related to glucose metabolism of P. aeruginosa (Figure 3 ). The reference sample was housekeeping gene proC ( Savli et al., 2003 ). FIGURE 3 Expression fold...”
- “...Glucose-6-phosphate isomerase), PA5110 ( fbp Fructose-1,6-bisphosphatase), PA3131 ( eda Aldolase), PA5192 ( pckA Phosphoenolpyruvate carboxykinase), PA5435 ( oadA Transcarboxylase subunit), PA1580 ( gltA Citrate synthase), PA2828 (probable aminotransferase ), proC (housekeeping Pyrroline-5-carboxylate reductase) in biofilm cells compared to planktonic cells. Each target gene was tested in...”
- The development of a new parameter for tracking post-transcriptional regulation allows the detailed map of the Pseudomonas aeruginosa Crc regulon
Corona, Scientific reports 2018 - “...ABC transporter 0,22 0,28 1,02 Catabolism aspA PA5429 Aspartate ammonia-lyase 0,05 0,6 1,76 CCM pycB PA5435 Probable transcarboxylase subunit 1,69 1,05 1,29 CCM pycA PA5436 Probable biotin carboxylase subunit of a transcarboxylase 1,09 0,88 1,4 Transport PA5504 D-methionine ABC transporter membrane protein 0,19 0,29 1,01 Catabolism...”
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- Identification of pyruvate carboxylase genes in Pseudomonas aeruginosa PAO1 and development of a P. aeruginosa-based overexpression system for alpha4- and alpha4beta4-type pyruvate carboxylases.
Lai, Applied and environmental microbiology 2006 - GeneRIF: The alpha4beta4 PYC (pyruvate carboxylase) of this organism was encoded by the ORFs PA5436 and PA5435.
PA14_71720 putative transcarboxylase subunit from Pseudomonas aeruginosa UCBPP-PA14
45% identity, 11% coverage
BAS3859 pyruvate carboxylase from Bacillus anthracis str. Sterne
GBAA4157 pyruvate carboxylase from Bacillus anthracis str. 'Ames Ancestor'
44% identity, 6% coverage
swp_1379 Oxaloacetate decarboxylase, alpha subunit from Shewanella piezotolerans WP3
44% identity, 10% coverage
- Condition-Specific Molecular Network Analysis Revealed That Flagellar Proteins Are Involved in Electron Transfer Processes of Shewanella piezotolerans WP3
Ding, Genetics research 2021 - “...conditions were mainly metabolism-related, including glutamate synthase subunit (swp_3803), pyruvate kinase II (swp_2388), oxaloacetate decarboxylase (swp_1379), multifunctional fatty acid oxidation complex subunit (swp_3139), 2-isopropylmalate synthase (swp_2213), isopropylmalate isomerase small subunit (swp_2216), chorismate mutase (swp_3758), Na + -transporting methylmalonyl-CoA/oxaloacetate decarboxylase subunit (swp_1380), sodium pump decarboxylase subunit (swp_1378),...”
- “...pressure Salt shock 1 swp_5124 swp_3803 swp_2623 swp_0485 2 swp_5105 swp_2388 swp_4312 swp_0361 3 swp_1504 swp_1379 swp_5025 swp_2579 4 swp_5095 swp_3139 swp_4352 swp_4934 5 swp_5110 swp_2213 swp_0430 swp_2897 6 swp_5111 swp_2216 swp_2139 swp_0501 7 swp_1508 swp_3758 swp_0854 swp_0495 8 swp_5087 swp_1380 swp_4933 swp_0486 9 swp_2950 swp_1378...”
Gbem_1454 oxaloacetate decarboxylase from Geobacter bemidjiensis Bem
40% identity, 10% coverage
- The genome of Geobacter bemidjiensis, exemplar for the subsurface clade of Geobacter species that predominate in Fe(III)-reducing subsurface environments
Aklujkar, BMC genomics 2010 - “...to propionyl-CoA by methylmalonyl-CoA decarboxylase (Gbem_0684) (Figure 2d ). The other oxaloacetate decarboxylase ( oadA Gbem_1454) is 60% identical to the catalytic subunit of the sodium-translocating oxaloacetate decarboxylase of Klebsiella pneumoniae [ 22 ]. No homologs of the other two subunits were found in G. bemidjiensis...”
- “...the oxaloacetate decarboxylase encoded by the gene adjacent to gcdA in G. bemidjiensis ( oadA Gbem_1454) contains two biotin attachment domains, whereas its sodium pump-associated homologs contain only one. The possibility that the two decarboxylases cooperate as a complex deserves investigation. Although the genome of G....”
O59021 149aa long hypothetical methylmalonyl-CoA decarboxylase gamma chain from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
PH1284 methylmalonyl-CoA decarboxylase gamma chain from Pyrococcus horikoshii OT3
44% identity, 44% coverage
- BEAN and HABAS: Polyphyletic insertions in the DNA-directed RNA polymerase.
Alvarez-Carreño, Protein science : a publication of the Protein Society 2024 - “...bD1 (A2BT61), D8 (Q0AUH3), psd (A0L627), cytochrome f (A2BPU4), peptidase M23 (A0A0E3QUJ6), RND (A0A1T5M7J5), BCC (O59021), OGDCE2 (P0AFG6), NusG (C5CGE4), rsxC (P77611) and uD5 (P11512). The structurederived multiple sequence alignment and the structure superimposition are colored by Scorecons conservation. Residues below 0.4 are gray. The location...”
- Crystallization and preliminary X-ray crystallographic studies of the biotin carboxyl carrier protein and biotin protein ligase complex from Pyrococcus horikoshii OT3
Bagautdinov, Acta crystallographica. Section F, Structural biology and crystallization communications 2007 - “...for providing plasmids and Dr M. Miyano for providing PH1284. We also thank the staff of beamline BL26B1 of SPring-8 for assistance during X-ray experiments....”
PCCA_MOUSE / Q91ZA3 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Mus musculus (Mouse) (see paper)
45% identity, 9% coverage
- function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl- CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)- ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+) (RHEA:59520)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits (By similarity). Interacts (via the biotin carboxylation domain) with SIRT4 (PubMed:23438705). Interacts with SIRT3 and SIRT5 (PubMed:23438705). - ISG15 Is a Novel Regulator of Lipid Metabolism during Vaccinia Virus Infection.
Albert, Microbiology spectrum 2022 - “...Q99MN9 Pccb Propionyl-CoA carboxylase beta chain, mitochondrial 10.02 P51174 Acadl Long-chain-specific acyl-CoA dehydrogenase, mitochondrial 8.64 Q91ZA3 Pcca Propionyl-CoA carboxylase alpha chain, mitochondrial 8.63 Q9EPL9 Acox3 Peroxisomal acyl-coenzyme A oxidase 3 8.62 Q8QZT1 Acat1 Acetyl-CoA acetyltransferase, mitochondrial 7.71 Q9Z2Z6 Slc25a20 Mitochondrial carnitine/acylcarnitine carrier protein 6.67 Q9JHI5 Ivd...”
- A Quantitative Proteomics Approach to Gain Insight into NRF2-KEAP1 Skeletal Muscle System and Its Cysteine Redox Regulation
Abu, Genes 2021 - “...Protein Log 2 FC Nrf2-KO Log 2 FC Keap1-KO Q9CXV1 Sdhd 1.87569 a 1.48549 b Q91ZA3 Pcca 1.69494 a 1.65623 b Q70IV5 Synm 1.67506 a 1.58577 b Q9JMH6 Txnrd1 1.38643 b 1.5322 a P15626 Gstm2 2.18014 b 1.4955 a Q9D6Y9 Gbe1 8.56657 b 3.97791 a a...”
- Action potential-coupled Rho GTPase signaling drives presynaptic plasticity
O'Neil, eLife 2021 - “...expression levels were intensity-scaled to the endogenously biotinylated proteins, pyruvate carboxylase (Q05920) and propionyl-CoA carboxylase (Q91ZA3). Imputation of missing values was performed after normalization ( Karpievitch et al., 2012 ) using the MinDet method ( Lazar et al., 2016 ). Missing values were replaced by the...”
- The role of cellular prion protein in lipid metabolism in the liver
Arora, Prion 2020 - “...155 1.59 0.031 5.16 4 Not identified 4.65 0.025 5 Propionyl-CoA carboxylase alpha chain, mitochondrial Q91ZA3 79.92 17.70% 17 1.58 0.04 6.04 Male 14-month-old mice 6 U5 small nuclear ribonucleoprotein 200 kDa helicase Q6P4T2 244.55 12.30% 36 1.77 0.012 5.73 7 3-mercaptopyruvate sulfurtransferase Q99J99 33.02 67.30%...”
- Environmental Enrichment Upregulates Striatal Synaptic Vesicle-Associated Proteins and Improves Motor Function
Song, Frontiers in neurology 2018 - “...gamma PIP5K1C 1.51 P32037 Solute carrier family 2, facilitated glucose transporter member 3 SLC2A3 1.51 Q91ZA3 Propionyl-CoA carboxylase alpha chain, mitochondrial PCCA 1.51 E9Q035 Protein Gm20425 GM20425 1.51 Q7TMY8-4 Isoform 4 of E3 ubiquitin-protein ligase HUWE1 HUWE1 1.51 D3YU23 Latrophilin-3 LPHN3 1.51 Q8C1A5 Thimet oligopeptidase THOP1...”
- Adenosine A1 receptor activation increases myocardial protein S-nitrosothiols and elicits protection from ischemia-reperfusion injury in male and female hearts
Shao, PloS one 2017 - “...1 P09542 191 85, 191 ND 85, 191 ND ND Propionyl CoA carboxylase alpha chain Q91ZA3 107 107 107 107 107 107 Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 O55143 349, 364, 447, 471 344, 349, 364, 447, 471, 635, 669 364, 635 344, 364, 447, 635, 998...”
- Characterization of the sex-dependent myocardial S-nitrosothiol proteome
Shao, American journal of physiology. Heart and circulatory physiology 2016 - “...75 (ND), 92 (ND), 727 (ND) 85 (ND) 85 (ND), 191 (ND) Q91ZA3 O55143 107 (ND) 344 (3.02) P07724 77 (ND), 289 (ND) 77 (ND), 289 (ND), 301 (ND), 302 (ND), 501 (ND),...”
- Apigenin Attenuates Atherogenesis through Inducing Macrophage Apoptosis via Inhibition of AKT Ser473 Phosphorylation and Downregulation of Plasminogen Activator Inhibitor-2.
Zeng, Oxidative medicine and cellular longevity 2015 - “...(25) 71270 6.23 Guanylate-binding protein 4 4 P12265 (BGLR_MOUSE) 143 (28) 74648 6.22 Beta-glucuronidase 5 Q91ZA3 (PCCA_MOUSE) 80 (27) 80498 6.83 Propionyl-CoA carboxylase alpha chain, mitochondrial 6 P58252 (EF2_MOUSE) 264 (27) 96222 6.41 Elongation factor 2 7 N/A e 8 P60710 (ACTB_MOUSE) 79 (26) 42052 5.29...”
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WP_048053351 biotin/lipoyl-containing protein from Pyrococcus horikoshii
44% identity, 45% coverage
A0A0G2K401 Propionyl-CoA carboxylase alpha chain, mitochondrial from Rattus norvegicus
45% identity, 10% coverage
H16_A2142 Acetyl-CoA carboxylase alpha chain from Ralstonia eutropha H16
H16_A2142 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Cupriavidus necator H16
38% identity, 10% coverage
CBG46_03830 sodium-extruding oxaloacetate decarboxylase subunit alpha from Actinobacillus succinogenes
42% identity, 11% coverage
ABD05_RS32735 urea carboxylase from Burkholderia pyrrocinia
42% identity, 5% coverage
Pf1N1B4_3984 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) from Pseudomonas fluorescens FW300-N1B4
42% identity, 10% coverage
- mutant phenotype: Specifically important for utilizing L-Leucine.
2ejfC / O59021 Crystal structure of the biotin protein ligase (mutations r48a and k111a) and biotin carboxyl carrier protein complex from pyrococcus horikoshii ot3 (see paper)
43% identity, 93% coverage
ESA_01768 hypothetical protein from Enterobacter sakazakii ATCC BAA-894
37% identity, 6% coverage
lpp0531 oxaloacetate decarboxylase alpha-chain from Legionella pneumophila str. Paris
38% identity, 11% coverage
- The Legionella pneumophila genome evolved to accommodate multiple regulatory mechanisms controlled by the CsrA-system
Sahr, PLoS genetics 2017 - “...Enolase, Pyk (Lpp0151), Pyruvate kinase, PpsA (Lpp0567), Phosphoenolpyruvate synthase, Pdh (Lpp1461), Pyruvate dehydrogenase complex, Pyc (Lpp0531), Pyruvate carboxyltransferase, Ppc (Lpp1572) Phosphoenolpyruvate carboxylase, SfcA (Lpp3043), NAD-specific malic enzyme, AcnA (LPP1659), Aconitate hydratase, Icd (Lpp0878), Isocitrate dehydrogenase, Sdh (Lpp0595), Succinate dehydrogenase, Suc (Lpp0597), 2-Oxoglutarate dehydrogenase, Ald (Lpp0986), Alanine...”
BCG_3341c Biotinylated protein TB7.3 from Mycobacterium bovis BCG str. Pasteur 1173P2
NP_217737 acetyl-CoA carboxylase biotin carboxyl carrier protein subunit from Mycobacterium tuberculosis H37Rv
BCG_3248c Biotinylated protein TB7.3 from Mycobacterium bovis BCG str. Pasteur 1173P2
Rv3221c hypothetical protein from Mycobacterium tuberculosis H37Rv
Mb3247c BIOTINYLATED PROTEIN TB7.3 from Mycobacterium bovis AF2122/97
42% identity, 88% coverage
- Comparison of the transcriptome, lipidome, and c-di-GMP production between BCGΔBCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages
Flores-Valdez, Scientific reports 2024 - “...Biotinylated protein TB7.3 contains acetyl-CoA carboxylase biotin carboxyl carrier protein subunit domain 0.791 0.013039128 TB7.3_2 BCG_3341c Rv3221c Biotinylated protein TB7.3 contains acetyl-CoA carboxylase biotin carboxyl carrier protein subunit domain 0.7188 0.019029613 BCG_2053 BCG_2053 Rv2034 Probable ArsR-type repressor protein 0.6551 0.018687862 BCG_1420 BCG_1420 Rv1358 Probable transcriptional regulatory...”
- Intrinsically disordered protein from a pathogenic mesophile Mycobacterium tuberculosis adopts structured conformation at high temperature.
Kumar, Proteins 2008 (PubMed)- GeneRIF: The intrinsically disordered state and biotin-binding feature of Rv3221c suggest that it may participate in many biochemical processes requiring biotin as a cofactor and adopt suitable conformations upon binding other folded targets.
- Comparison of the transcriptome, lipidome, and c-di-GMP production between BCGΔBCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages
Flores-Valdez, Scientific reports 2024 - “...Rv1091 PE-PGRS family protein 0.8331 0.028777497 PE_PGRS45b BCG_2641c Rv2615c PE-PGRS family protein 0.7918 0.012061469 TB7.3_1 BCG_3248c Rv3221c Biotinylated protein TB7.3 contains acetyl-CoA carboxylase biotin carboxyl carrier protein subunit domain 0.791 0.013039128 TB7.3_2 BCG_3341c Rv3221c Biotinylated protein TB7.3 contains acetyl-CoA carboxylase biotin carboxyl carrier protein subunit domain...”
- Comparison of the transcriptome, lipidome, and c-di-GMP production between BCGΔBCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages
Flores-Valdez, Scientific reports 2024 - “...PE-PGRS family protein 0.8331 0.028777497 PE_PGRS45b BCG_2641c Rv2615c PE-PGRS family protein 0.7918 0.012061469 TB7.3_1 BCG_3248c Rv3221c Biotinylated protein TB7.3 contains acetyl-CoA carboxylase biotin carboxyl carrier protein subunit domain 0.791 0.013039128 TB7.3_2 BCG_3341c Rv3221c Biotinylated protein TB7.3 contains acetyl-CoA carboxylase biotin carboxyl carrier protein subunit domain 0.7188...”
- A Phenotypic Characterization of Two Isolates of a Multidrug-Resistant Outbreak Strain of Mycobacterium tuberculosis with Opposite Epidemiological Fitness
Bei, BioMed research international 2020 - “...three proteins from the DevR regulon (Rv0569, Rv2031c, and Rv2007c), two proteins of unknown functions (Rv3221c and Rv2081c), a GCN5-related N-acetyltransferase (Eis, Rv2416c), and a putative ribonuclease E (Rv2444c). Rv2057c, Rv2058c, Rv2154c, and Rv0290 showed a higher accumulation in Mp. Rv2057c and Rv2058c are ribosomal proteins,...”
- “...Mp and 410 strains. Protein Rv number Log 2 fold change a P value Description Rv3221c Rv3221c -2.78 8.20 E -03 Biotinylated protein IdsA2 Rv2173 -2.57 1.20 E -03 Probable geranylgeranyl pyrophosphate synthetase Rv0569 Rv0569 -2.55 1.90 E -03 Uncharacterized protein hspX Rv2031c -2.52 1.20 E...”
- Role of VapBC12 Toxin-Antitoxin Locus in Cholesterol-Induced Mycobacterial Persistence
Talwar, mSystems 2020 - “...Lipid metabolism 60 Rv0972c Lipid metabolism 60 Rv2724c Lipid metabolism 60 Rv2982c Lipid metabolism 60 Rv3221c Lipid metabolism 100 Rv0771 Intermediary metabolism and respiration 60 Rv1851 Intermediary metabolism and respiration 60 Rv1826 Intermediary metabolism and respiration 60 Rv2499c Intermediary metabolism and respiration 60 Rv2511 Intermediary metabolism...”
- A High Throughput Whole Blood Assay for Analysis of Multiple Antigen-Specific T Cell Responses in Human Mycobacterium tuberculosis Infection
Whatney, Journal of immunology (Baltimore, Md. : 1950) 2018 - “...51 Rv3135 PPE50 PPE family Unknown Pool 52 Rv3136 PPE51 PPE family Membrane Pool 53 Rv3221c Tb7.3 5 Secreted Pool 54 Rv3330 DacB1 1 Cell wall Pool 55 Rv3418c GroES 3 Cytosol, cell wall Pool 56 Rv3615c EspC 1 Membrane Pool 57 Rv3804c Ag85A 5 Secreted...”
- A systematic review of East African-Indian family of Mycobacterium tuberculosis in Brazil
Duarte, The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases 2017 - “...Rv3077 Rv3077_1002a>G S 34 3455686 Rv3088 Rv3088_1347g>C S 34 3544710 Rv3176c Rv3176c_591a>G S 34 3597737 Rv3221c Rv3221c_30g>A S 33 3641447 Rv3261 Rv3261_905c>T NS 33 3681548 Rv3297 Rv3297_229a>C S 13 3783058 Rv3370c Rv3370c_1683c>T S 34 4024273 Rv3581c Rv3581c_75a>G S 34 4081987 Rv3644c Rv3644c_735c>G S 13 4081996 Rv3644c...”
- Evaluation of spoligotyping, SNPs and customised MIRU-VNTR combination for genotyping Mycobacterium tuberculosis clinical isolates in Madagascar
Rasoahanitralisoa, PloS one 2017 - “...This method is performed to screen SNPs on six genomic regions ( katG 463, Rv2952, Rv3221c, Rv3804c, gyrA 1842 and Psts 1) S1 Table . Briefly, 10 ng of DNA was added to a final volume of 12l containing 5l TaqMan Universal Master Mix II (Qiagen),...”
- B in TB: B Cells as Mediators of Clinically Relevant Immune Responses in Tuberculosis
Rao, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2015 - “...51 O53673 Heat shock protein (heat-stress-induced ribosome-binding protein A) Rv0251c 52 P0A510 Biotinylated protein TB7.3 Rv3221c Proteins involved in transcriptional regulation of Mtb 53 O06153 Universal stress protein Rv1636/MT1672 (USP Rv1636) Rv1636 54 O06189 Universal stress protein Rv2623/MT2698 (USP Rv2623) Rv2623 55 P95193 Transcriptional regulatory protein...”
- Genome-wide discovery of small RNAs in Mycobacterium tuberculosis
Miotto, PloS one 2012 - “...e 3597808 3598107 + 299 150 sigA Yes g AS to 3, Rv3221A or 5, Rv3221c 540 f 4025441 4025570 + 129 70 AS to 5 UTR, Rv3583c 1029 732392 732787 395 Neg sigA AS to Rv0636 1080 e 937956 938239 283 230 sigA AS to...”
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- Genomic analysis of a Mycobacterium bovis bacillus [corrected] Calmette-Guérin strain isolated from an adult patient with pulmonary tuberculosis.
Li, PloS one 2015 - “...WhiB Mb3220 ABC transporter ATP-binding protein Mb3246c two component sensor kinase Mb3222c DNA helicase II Mb3247c acetyl- CoA carboxylase biotin carboxyl carrier protein subunit Mb3223 glutaredoxin protein Mb3248c anti-sigma factor Mb3224c NADH pyrophosphatase Mb3249c hypothetical protein Mb3225c transmembrane cation transporter Mb3250c RNA polymerase sigma factor RpoE...”
MT3317 hypothetical protein from Mycobacterium tuberculosis CDC1551
42% identity, 85% coverage
MCA_RS12165 sodium-extruding oxaloacetate decarboxylase subunit alpha from Methylococcus capsulatus str. Bath
42% identity, 11% coverage
MM1827 Pyruvate carboxylase subunit B from Methanosarcina mazei Goe1
44% identity, 12% coverage
- S-layer, surface-accessible, and concanavalin A binding proteins of Methanosarcina acetivorans and Methanosarcina mazei
Francoleon, Journal of proteome research 2009 - “...pyruvate carboxylase subunit B (oxaloacetate decarboxylase) in both Methanosarcina species ( i.e. , MA0674 and MM1827). This endogenously biotinylated subunit is homologous to the soluble biotin-containing subunit PYCB of Methanosarcina barkeri that is part of the 4 4 -type acetyl CoA-independent pyruvate carboxylase (PYC) complex. This...”
- “...74.0 7 yes 1 MM1770 pyruvate phosphate dikinase 38 97.0 3 no 0 Band 4 MM1827 pyruvate carboxylase, subunit B 136 63.3 4 no 0 MM0001 dipeptide ABC transporter, binding protein 56 58.1 2 no 0 Band 5 MM1096 thermosome, gamma subunit 221 58.2 8 no...”
TON_0904 oxaloacetate decarboxylase, alpha subunit from Thermococcus onnurineus NA1
44% identity, 11% coverage
SC0049 putative sodium ion pump oxaloacetate decarboxylase alpha chain from Salmonella enterica subsp. enterica serovar Choleraesuis str. SC-B67
42% identity, 11% coverage
C1DH60 Pyruvate carboxylase subunit B from Azotobacter vinelandii (strain DJ / ATCC BAA-1303)
45% identity, 11% coverage
- Nitrogenase cofactor biosynthesis using proteins produced in mitochondria of <i>Saccharomyces cerevisiae</i>
Dobrzyńska, mBio 2024 - “...by peptide mass fingerprinting [1, 6, 8, and 9, pyruvate carboxylase subunit B (PycB, UniProt C1DH60); 2, 3, and 4, degradation products of Av NifN Ss ; and 5, 7, and 10, biotin carboxyl carrier protein of acetyl-CoA carboxylase (AccB, UniProt C1DLJ8)]. Intact Av NifE Ht...”
TC 3.B.1.1.1 / Q03030 DCOA aka OADA1 aka OADA aka STM0055 aka OADA2 aka STM3352, component of Na+-transporting oxalo-acetate decarboxylase. Subunit stoichiometries have been described (Balsera et al., 2011). The crystal structure of the carboxyltransferase at 1.7 A resolution shows a dimer of alpha(8)beta(8) barrels with an active site metal ion, identified spectroscopically as Zn2+ from Salmonella typhimurium (see 2 papers)
STM0055 putative oxalacetate decarboxylase, subunit alpha from Salmonella typhimurium LT2
42% identity, 11% coverage
- substrates: Na+
- Salmonella serovar identification using PCR-based detection of gene presence and absence
Arrach, Journal of clinical microbiology 2008 - “...STM0024, STM0030, STM0032, STM0036, STM0042, STM0051, STM0055, STM0061, STM0063, STM0073, STM0077, STM0162, STM0163, STM0175, STM0197, STM0256, STM0257,...”
SL1344_3324 sodium-extruding oxaloacetate decarboxylase subunit alpha from Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
42% identity, 11% coverage
D0C8Q7 Urea carboxylase from Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81)
37% identity, 6% coverage
A0A1U7EYC1 biotin carboxylase (EC 6.3.4.14) from Natronomonas pharaonis (see paper)
45% identity, 11% coverage
Pfl01_3659 3-methylcrotonoyl-CoA carboxylase, alpha subunit from Pseudomonas fluorescens Pf0-1
40% identity, 10% coverage
pyr1 / RF|NP_595900.1 pyruvate carboxylase Pyr1; EC 6.4.1.1 from Schizosaccharomyces pombe (see paper)
SPBC17G9.11c pyruvate carboxylase from Schizosaccharomyces pombe
42% identity, 5% coverage
Teth39_0307 biotin/lipoyl attachment domain-containing protein from Thermoanaerobacter ethanolicus ATCC 33223
40% identity, 51% coverage
lpg0466 oxaloacetate decarboxylase alpha subunit from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
36% identity, 11% coverage
- New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila
Ge, Molecular & cellular proteomics : MCP 2022 - “...77.4 8.95 Type IV pilus biogenesis protein PilQ Lpg0469 lpg0469 29.5 8.88 Phosphatase family protein Lpg0466 lpg0466 66.4 5.64 Oxaloacetate decarboxylase alpha subunit BamE lpg0372 13.2 10.33 Small protein A, tmRNA-binding DsbD lpg0686 65.3 8.68 Thiol:disulfide interchange protein DsbD Lpg0594 lpg0594 7.2 3.93 Uncharacterized protein FrgA...”
- Legionella Pneumophila Transcriptome during Intracellular Multiplication in Human Macrophages
Faucher, Frontiers in microbiology 2011 - “...regulated compared to growth in rich broth. We also noted the induction of lpg1607 and lpg0466 that are predicted to encode enzymes that would mediate oxaloacetate production, from phospho-enolpyruvate and pyruvate respectively, which can then be used in the TCA cycle or for the production of...”
- “...L. pneumophila also uses glycerol as a carbon source and that carboxylation of pyruvate by Lpg0466 or phospho- enol -pyruvate by Lpg1607 provide oxaloacetate that could then be use for the biosynthesis of l -aspartate and l -lysine (Figure 6 ), but it will need further...”
VT47_11875 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Pseudomonas syringae pv. syringae
42% identity, 10% coverage
- Identification of the Genes of the Plant Pathogen Pseudomonas syringae MB03 Required for the Nematicidal Activity Against Caenorhabditis elegans Through an Integrated Approach
Ali, Frontiers in microbiology 2022 - “...stationary phase interaction: lysR transcriptional regulator (VT47_13020), isovaleryl-CoA dehydrogenase (VT47_11890), putative acyl-CoA carboxylase alpha chain (VT47_11875), acyl-CoA carboxyltransferase beta chain (VT47_11885), prpC (VT47_09990), and PrpB (VT47_09985). On the other hand, crfX (VT47_10030), (VT47_19750) and hypothetical protein (VT47_10035) were down regulated ( Table 2 ). Regarding nematicidal...”
- “...h VT47_13020 Yes Unchanged Up LysR family transcriptional regulator |VT47_11890 Yes Unchanged Up Isovaleryl-CoA dehydrogenase VT47_11875 Yes Unchanged Up 3-Methylcrotonyl-CoA carboxylase alpha subunit VT47_11885 Yes Unchanged Up Propionyl-CoA carboxylase prpC VT47_09990 Yes Unchanged Up 2-Methylcitrate synthase prpB VT47_09985 Yes Unchanged Up 2-Methylisocitrate lyase cmpX VT47_10035 Yes...”
XP_002183906 precursor of carboxylase pyruvate carboxylase from Phaeodactylum tricornutum CCAP 1055/1
B7GA98 pyruvate carboxylase from Phaeodactylum tricornutum (strain CCAP 1055/1)
44% identity, 5% coverage
PST_3216 acetyl-CoA carboxylase, biotin carboxylase, putative from Pseudomonas stutzeri A1501
42% identity, 10% coverage
- Poly-3-hydroxybutyrate production from acetate by recombinant Pseudomonas stutzeri with blocked L-leucine catabolism and enhanced growth in acetate
Zhu, Frontiers in bioengineering and biotechnology 2023 - “...Isovaleryl-CoA is eventually transformed into acetyl-CoA and acetoacetate via pst_3213 , pst_3214 , pst_3215 , pst_3216 , and pst_3217 , while acetoacetate is catabolized into acetoacetyl-CoA by pst_3218 and pst_3219 and finally degraded to acetyl-CoA. Besides pst_3217 , other genes involved in isovaleryl-CoA catabolism including pst_3213,...”
- “...-leucine in P. stutzeri A1501. PST_3213, isovaleryl-CoA dehydrogenase; PST_3214, methylcrotonyl-CoA carboxylase, subunit; PST_3215, -methylglutaconyl-CoA hydratase; PST_3216, methylcrotonyl-CoA carboxylase, subunit; PST_3217, hydroxymethylglutaryl-CoA lyase; PST_3218, CoA transferase, subunit A; PST_3219, CoA transferase, subunit B. (C) The growth of different strains in AB minimal medium with 40mM acetate. (D)...”
SMUL_0789 biotin/lipoyl-containing protein from Sulfurospirillum multivorans DSM 12446
40% identity, 10% coverage
Q612F5 Propionyl-CoA carboxylase alpha chain, mitochondrial from Caenorhabditis briggsae
45% identity, 8% coverage
PCCA_CAEEL / Q19842 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Caenorhabditis elegans (see paper)
Q19842 propionyl-CoA carboxylase (subunit 2/2) (EC 6.4.1.3) from Caenorhabditis elegans (see paper)
NP_509293 Propionyl-CoA carboxylase alpha chain, mitochondrial from Caenorhabditis elegans
45% identity, 9% coverage
- function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl- CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)- ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+) (RHEA:59520)
cofactor: biotin
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
subunit: The holoenzyme is a dodecamer composed of 6 alpha subunits and 6 beta subunits (By similarity). Interacts with sir-2.2 and sir-2.3 (PubMed:23438705). - Propionyl-CoA carboxylase pcca-1 and pccb-1 gene deletions in Caenorhabditis elegans globally impair mitochondrial energy metabolism.
Chapman, Journal of inherited metabolic disease 2018 - GeneRIF: Two C. elegans model animals of propionic acidemia with single-gene pcca-1 or pccb-1 deletions have reduced lifespan with significantly reduced mitochondrial energy metabolism and increased oxidative stress.
- Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti--identification of stage-specific proteases
Soblik, Molecular & cellular proteomics : MCP 2011 - “...2e 104 No 317 12.0 3 9.17 25 SR00383 Propionyl Coenzyme A Carboxylase C. elegans NP_509293 2e 17 No 76 50.0 3 9.10 1 SR01608 EF hand family protein Brugia malayi XP_001901161 2e 37 Yes 158 69.0 8 23.28 2 SR03191 Prolyl endopeptidase Rattus norvegicus EDL99674...”
- Altered proteostasis in aging and heat shock response in C. elegans revealed by analysis of the global and de novo synthesized proteome
Liang, Cellular and molecular life sciences : CMLS 2014 - “...0.8 0.4 Q22111 mmaa-1 Methylmalonic aciduria type A protein 4 0.7 0.4 4 0.8 0.3 Q19842 pcca-1 Propionyl coenzyme-A carboxylase alpha subunit 4 0.8 0.6 Q9U2M4 Y38F1A.6 Ortholog, human isoform 1, HOT 1 0.6 0.3 Q9U2M4 Y38F1A.6 Ortholog, human isoform 1, HOT 5 0.8 0.3 Ribosomal...”
- Proteomic analysis of Oesophagostomum dentatum (Nematoda) during larval transition, and the effects of hydrolase inhibitors on development
Ondrovics, PloS one 2013 - “...Cyathostominae A7LGW9 741 83 9*10 77 2 / Propionyl-CoA carboxylase alpha chain / Caenorhabditis elegans Q19842 / / / 3 / Propionyl-CoA carboxylase alpha chain / C. elegans Q19842 / / / 4+5 Oden_isotig22486 Phosphoenolpyruvate carboxy kinase GTP Putative uncharacterized protein C. briggsae A8WMQ5 787 80...”
PAAG_02163 acetyl-/propionyl-coenzyme A carboxylase alpha chain from Paracoccidioides lutzii Pb01
38% identity, 5% coverage
- Interaction of Isocitrate Lyase with Proteins Involved in the Energetic Metabolism in Paracoccidioides lutzii
Freitas, Journal of fungi (Basel, Switzerland) 2020 - “...2-methylisocitrate lyase 11,769.04 PAAG_04550 2-methylcitrate synthase * 13,649.55 PAAG_04559 2-methylcitrate dehydratase * 13,423.09 Propionate metabolism PAAG_02163 propionyl-CoA carboxylase 11,642.86 Pentose-phosphate pathway PAAG_04444 transketolase 9554.287 PAAG_04166 transaldolase 9647.347 Glyoxylate cycle PAAG_04542 malate synthase 7841.01 PAAG_07786 acetyl-CoA acetyltransferase 9100.539 Energy conversion PAAG_03631 12-oxophytodienoate reductase * 10,708.71 Functional classification...”
- Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
Bailão, PLoS neglected tropical diseases 2014 - “...AVG Peptides AVG Fold change (Hb:Fe) E.C. number Subclassification Filter b METABOLISM Amino acid metabolismo PAAG_02163 acetyl-/propionyl-coenzyme A carboxylase alpha chain 249.65 16.33 *** 6.4.1.3 Valine and isoleucine degradation 1 PAAG_07036 methylmalonate-semialdehyde dehydrogenase 279.14 14.83 1.60 1.2.1.27 Valine, leucine and isoleucine degradation 2 PAAG_00221 acetolactate synthase...”
- Transcriptional and proteomic responses to carbon starvation in Paracoccidioides
Lima, PLoS neglected tropical diseases 2014 - “...PAAG_03316 polysaccharide export protein # surface polysaccharide biosynthesis process Lipid, fatty acid and isoprenoid metabolism PAAG_02163 acetyl-/propionyl-coenzyme A carboxylase alpha chain 3.03 lipid metabolism PAAG_05249 aldehyde dehydrogenase 2.45 lipid and fatty acid metabolism_oxidation PAAG_01928 peroxisomal dehydratase # Lipid, fatty acid and isoprenoid metabolism PAAG_06099 glycerol-3-phosphate dehydrogenase...”
MCP_0183 pyruvate carboxylase subunit B from Methanocella paludicola SANAE
47% identity, 11% coverage
PSPTO_2736 biotin carboxylase/biotin-containing subunit from Pseudomonas syringae pv. tomato str. DC3000
40% identity, 10% coverage
- A computational model of Pseudomonas syringae metabolism unveils a role for branched-chain amino acids in Arabidopsis leaf colonization
Tubergen, PLoS computational biology 2023 - “...in BCAAs biosynthesis. The leucine minimal medium rendered three additional genes predicted to be essential: PSPTO_2736, PSPTO_2738, and PSPTO_2739. The three genes show high sequence homology to the leucine catabolic genes liuD , liuB , and liuA , respectively. 10.1371/journal.pcbi.1011651.g003 Fig 3 iPst19 predicts genes of...”
- “...one iso-propyl-malate dehydrogenase (PSPTO_2175) were essential to metabolize glucose, three genes of the liu operon (PSPTO_2736, PSPTO_2738, and PSPTO_2739) were essential to use leucine as the sole carbon source. (C) Predicted gene essentiality changes with mixed carbon sources where iPst19 gene essentiality was simulated in conditions...”
Pcca / P14882 propionyl-CoA carboxylase α subunit (EC 6.4.1.3) from Rattus norvegicus (see paper)
P14882 Propionyl-CoA carboxylase alpha chain, mitochondrial from Rattus norvegicus
49% identity, 7% coverage
- Proteomic mapping reveals dysregulated angiogenesis in the cerebral arteries of rats with early-onset hypertension
Bastrup, The Journal of biological chemistry 2023 - “...and casein kinase substrate in neurons 2 protein 0.32 13.94 1.51E-04 1.12E-02 Pcca Isoform of P14882, propanoyl-CoA:carbon dioxide ligase subunit alpha; propionyl-CoA carboxylase alpha chain, mitochondrial 0.20 15.31 5.55E-04 2.53E-02 Pccb Propionyl-CoA carboxylase beta chain, mitochondrial; isoform of P07633, propionyl coenzyme A carboxylase, beta polypeptide 0.26...”
- Extracellular vesicles released by steatotic hepatocytes alter adipocyte metabolism
Mleczko, Journal of extracellular biology 2022 - “...CP2CN_RAT P24470 1.75 8.02E03 Cytochrome P450 2C23 PDIA3_RAT P11598 1.75 5.38E05 Protein disulfideisomerase A3 PCCA_RAT P14882 1.77 1.95E03 PropionylCoA carboxylase alpha chain, mitochondrial ANGL4_RAT Q6TMA8 1.77 3.93E03 Angiopoietinrelated protein 4 HMGCL_RAT P97519 1.78 1.01E03 HydroxymethylglutarylCoA lyase, mitochondrial SDC4_RAT P34901 1.78 1.17E04 Syndecan4 CP2DQ_RAT P10634 1.79 1.03E03...”
- Proteomic analysis of spinal cord tissue in a rat model of cancer-induced bone pain.
Yang, Frontiers in molecular neuroscience 2022 - “...E9PU28 Impdh2 0.13 3.0 Down Q6IFU8 Krt17 0.13 3.0 Down Q6KC51 Ablim2 0.13 3.0 Down P14882 Pcca 0.13 3.0 Down Q5M819 Psph 0.13 3.0 Down Q704S8 Crat 0.13 2.9 Down P63269 Actg2 0.13 2.9 Down P62738 Acta2 0.13 2.9 Down Functional classification of differentially expressed protein...”
- Functional Redox Proteomics Reveal That Salvia miltiorrhiza Aqueous Extract Alleviates Adriamycin-Induced Cardiomyopathy via Inhibiting ROS-Dependent Apoptosis.
Hung, Oxidative medicine and cellular longevity 2020 - “...May play a role in the control of cell proliferation and cellular aging. 3 PCCA P14882 82.198/7.59 113 (32%) 16 Mitochondrion matrix This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty...”
- Functional proteomic analysis of corticosteroid pharmacodynamics in rat liver: Relationship to hepatic stress, signaling, energy regulation, and drug metabolism.
Ayyar, Journal of proteomics 2017 - “...UP P57093 Phyh Phytanoyl-CoA dioxygenase, peroxisomal Fatty acid metabolism - converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA UP P14882 Pcca Propionyl-CoA carboxylase alpha chain, mitochondrial Fatty acid beta oxidation - catalyses the carboxylation reaction of propionyl CoA to form (S)-methylmalonyl CoA UP P70584 Acadsb Short/branched chain acyl-CoA dehydrogenase, mitochondrial...”
- The Proteome Profiles of the Cerebellum of Juvenile, Adult and Aged Rats--An Ontogenetic Study
Wille, International journal of molecular sciences 2015 - “...Gmps GMP synthase (glutamine-hydrolyzing) Cp up 0.0062 - - 0.52 5 130 17 77,507 6.21 P14882 PCCA_RAT Pcca Propionyl-CoA carboxylase alpha chain, mitochondrial Mmt down 0.0283 - - 2.14 5 262 32 82,198 7.59 Fat Metabolism Q5XI22 THIC_RAT Acat2 Acetyl-CoA acetyltransferase, cytosolic Cp - - up...”
- Identification of εPKC targets during cardiac ischemic injury
Budas, Circulation journal : official journal of the Japanese Circulation Society 2012 - “...225 83kDa 6.18 75kDa 6.35 5.1 mitochondria 0.02* 8 Propionyl-CoA carboxylase alpha chain, mitochondrial precursor P14882 12 78 77kDa 6.33 69kDa 6.41 5.1 mitochondria 0.02* 9 Dihydrolipoyl dehydrogenase, mitochondrial precursor Q6P6R2 13 207 54kDa 7.96 48kDa 7.33 4.1 mitochondria 0.01* 10 ATP synthase subunit alpha, mitochondrial...”
- Use of biotinylated ubiquitin for analysis of rat brain mitochondrial proteome and interactome.
Buneeva, International journal of molecular sciences 2012 - “...beta chain, mitochondrial precursor P07633 13 67.94 M 2 Propionyl-CoA carboxylase alpha chain, mitochondrial precursor P14882 5 37.33 M Transporters ( n = 4) 1 Excitatory amino acid transporter 1 P24942 6 34.57 PM 2 Excitatory amino acid transporter 2 P31596 2 26.35 PM 3 Glial...”
- “...P26284, Dlat = P08461, Hspd1 = P63039, Hspe1 = P26772, Slc25a5 = Q09073, Pcca = P14882, Pccb = P07633, Atp1a2 = P06686, Atp1a1 = P06685, Nefm = P12839, Nefl = P19527, Camk2a = P11275, Ina = P23565, Gfap = P47819, Slc1a3 = P24942, ENSRNOG00000018630 = Q0QEU1,...”
- More
BV82_2132 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Pseudomonas donghuensis
42% identity, 10% coverage
2OCL_HYDTT / D3DJ41 2-oxoglutarate carboxylase large subunit; 2-oxoglutarate carboxylase alpha subunit; EC 6.4.1.7 from Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6) (see 2 papers)
45% identity, 10% coverage
- catalytic activity: hydrogencarbonate + 2-oxoglutarate + ATP = (S)-oxalosuccinate + ADP + phosphate + H(+) (RHEA:20425)
cofactor: Mg(2+) Mn(2+) Co(2+)
subunit: Heterohexadecamer of 8 large subunits and 8 small subunits.
A1S_1278 Allophanate hydrolase subunit 2 from Acinetobacter baumannii ATCC 17978
37% identity, 32% coverage
- Whole transcriptome analysis of Acinetobacter baumannii assessed by RNA-sequencing reveals different mRNA expression profiles in biofilm compared to planktonic cells
Rumbo-Feal, PloS one 2013 - “...coding an acyl carrier protein, an allophanate hydrolase and the RND efflux pump AdeT (A1S_0114, A1S_1278 and A1S_1755, respectively) were only expressed in biofilms and were totally inhibited in planktonic cells. Genes corresponding to hypothetical proteins (A1S_0302, A1S_0644, A1S_1293, and A1S_2893), a transmembrane arsenate pump protein...”
- “...A1S_1077 hypothetical protein 9.41 2.43 A1S_1104 chlorogenate esterase 0.35 0.01 A1S_1266 hypothetical protein 1.09 11.88 A1S_1278 allophanate hydrolase subunit 2 from zero to 20.35 ** from zero to 20.35 ** A1S_1293 hypothetical protein 15.25 from zero to 30.54 ** A1S_1316 major facilitator superfamily transporter cyanate permease...”
XAC4326 urea amidolyase from Xanthomonas axonopodis pv. citri str. 306
39% identity, 5% coverage
SACE_3400 acetyl/propionyl-CoA carboxylase alpha subunit from Saccharopolyspora erythraea NRRL 2338
38% identity, 12% coverage
- Engineering of a TetR family transcriptional regulator BkdR enhances heterologous spinosad production in <i>Streptomyces albus</i> B4 chassis
Wang, Applied and environmental microbiology 2024 (secret) - Polyketide Starter and Extender Units Serve as Regulatory Ligands to Coordinate the Biosynthesis of Antibiotics in Actinomycetes
Wu, mBio 2021 - “...29 ). SACE_0026-0028 and SACE_3856 genes encode ACC enzymes; SACE_3398-3399 , SACE_7038-7039 , SACE_3241-3242 , SACE_3400 , SACE_4237 , and SACE_6509 genes encode ACC and/or PCC enzymes; SACE_0632 , SACE_0633 , and SACE_0649 genes encode CS enzymes. Mean values of 3 measurements are shown with SDs....”
- “...eight sets of putative ACC and/or PCC genes ( SACE_0026-0028 , SACE_3241-3242 , SACE_3398-3399 , SACE_3400 , SACE_3856 , SACE_4237 , SACE_6509 , and SACE_7038-7039 ) and three citrate synthase (CS) genes ( SACE_0632 , SACE_0633 , and SACE_0649 ) between A226 and A226 acrT (...”
- The Expression of NOX From Synthetic Promoters Reveals an Important Role of the Redox Status in Regulating Secondary Metabolism of Saccharopolyspora erythraea
Li, Frontiers in bioengineering and biotechnology 2020 - “...production in the NOX-expression strains at transcription level ( Liao et al., 2015 ). Furthermore SACE_3400 and SACE_6509 coding for acetyl-CoA carboxylase were repressed gradually in the NOX-expression strains. The repression of SACE_3400 and SACE_6509 could reduce the accumulation of malonyl-CoA from acetyl-CoA. The transcriptional changes...”
- PccD Regulates Branched-Chain Amino Acid Degradation and Exerts a Negative Effect on Erythromycin Production in Saccharopolyspora erythraea
Xu, Applied and environmental microbiology 2018 - “...Environmental Microbiology the promoter sequence of pccA (SACE_3400) using MEME (http://meme-suite.org/) (29). Based on the predicted PccD-binding motif, we...”
- TetR Family Transcriptional Regulator PccD Negatively Controls Propionyl Coenzyme A Assimilation in Saccharopolyspora erythraea
Xu, Journal of bacteriology 2017 - “...operon: pccBC), and wasn't 122 cotranscribed with the pccA (SACE_3400). So far, it is still unclear which of the 123 possible propionyl-CoA carboxylases make a...”
- “...604 605 Figure 1. pccBC (SACE_3398-3399) and pccA (SACE_3400) operons are induced by propanol and propionate in S. erythraea. A. In S. erythraea, branched-chain...”
PSPPH_5142 oxaloacetate decarboxylase alpha subunit from Pseudomonas syringae pv. phaseolicola 1448A
45% identity, 11% coverage
jk1669 acyl-CoA carboxylase, alpha subunit from Corynebacterium jeikeium K411
38% identity, 12% coverage
PF0673 methylmalonyl-CoAdecarboxylase gamma chain from Pyrococcus furiosus DSM 3638
42% identity, 46% coverage
PTH_1366 methylmalonyl-CoA decarboxylase, gamma subunit from Pelotomaculum thermopropionicum SI
42% identity, 45% coverage
Mal4_02890 pyruvate carboxylase from Maioricimonas rarisocia
39% identity, 6% coverage
BCAS0272 urea amidolyase, urea carboxylase subunit from Burkholderia cenocepacia J2315
38% identity, 5% coverage
- NtrC-dependent control of exopolysaccharide synthesis and motility in Burkholderia cenocepacia H111
Liu, PloS one 2017 - “...arginine/histidine transport system,permease component -5.2 I35_7110 BCAS0113 Histidine ABC transporter, ATP-binding protein hisP -3.8 I35_7286 BCAS0272 Urea carboxylase -6.0 I35_7447 BCAS0409 Zinc metalloprotease zmpA 2.4 I35_7737 BCAS0574 Glutamate transport ATP-binding protein -4.3 I35_7739 BCAS0576 amino acid ABC transporter, permease protein na(m) I35_7740 BCAS0577 ABC-type amino acid...”
WP_030405160 biotin/lipoyl-binding carrier protein from Streptomyces sp. NRRL F-5555
41% identity, 86% coverage
APL_1376 oxaloacetate decarboxylase alpha chain from Actinobacillus pleuropneumoniae L20
45% identity, 11% coverage
DUR1_LACKL / A5H0J2 Urea amidolyase; Pyrimidine-degrading protein 13,15; Uracil catabolism protein 3,5; EC 6.3.4.6; EC 3.5.1.54 from Lachancea kluyveri (Yeast) (Saccharomyces kluyveri) (see paper)
40% identity, 3% coverage
- function: Involved in uracil catabolism. Hydrolysis of urea to ammonia and CO(2).
catalytic activity: urea + hydrogencarbonate + ATP = urea-1-carboxylate + ADP + phosphate + H(+) (RHEA:20896)
catalytic activity: urea-1-carboxylate + H2O + 3 H(+) = 2 NH4(+) + 2 CO2 (RHEA:19029)
cofactor: biotin
subunit: Monomer.
PFL_6157 oxaloacetate decarboxylase alpha subunit from Pseudomonas fluorescens Pf-5
44% identity, 11% coverage
SACE_6509 acetyl/propionyl-CoA carboxylase alpha subunit from Saccharopolyspora erythraea NRRL 2338
38% identity, 11% coverage
- Polyketide Starter and Extender Units Serve as Regulatory Ligands to Coordinate the Biosynthesis of Antibiotics in Actinomycetes
Wu, mBio 2021 - “...genes encode ACC enzymes; SACE_3398-3399 , SACE_7038-7039 , SACE_3241-3242 , SACE_3400 , SACE_4237 , and SACE_6509 genes encode ACC and/or PCC enzymes; SACE_0632 , SACE_0633 , and SACE_0649 genes encode CS enzymes. Mean values of 3 measurements are shown with SDs. *, P< 0.05; **, P<...”
- “...PCC genes ( SACE_0026-0028 , SACE_3241-3242 , SACE_3398-3399 , SACE_3400 , SACE_3856 , SACE_4237 , SACE_6509 , and SACE_7038-7039 ) and three citrate synthase (CS) genes ( SACE_0632 , SACE_0633 , and SACE_0649 ) between A226 and A226 acrT ( 28 , 29 ). The results...”
- The Expression of NOX From Synthetic Promoters Reveals an Important Role of the Redox Status in Regulating Secondary Metabolism of Saccharopolyspora erythraea
Li, Frontiers in bioengineering and biotechnology 2020 - “...the NOX-expression strains at transcription level ( Liao et al., 2015 ). Furthermore SACE_3400 and SACE_6509 coding for acetyl-CoA carboxylase were repressed gradually in the NOX-expression strains. The repression of SACE_3400 and SACE_6509 could reduce the accumulation of malonyl-CoA from acetyl-CoA. The transcriptional changes of these...”
- TetR Family Transcriptional Regulator PccD Negatively Controls Propionyl Coenzyme A Assimilation in Saccharopolyspora erythraea
Xu, Journal of bacteriology 2017 - “...carboxylases are 118 annotated, 119 SACE_7038-7039, SACE_4237, and SACE_6509 (Fig. S1). The co-transcription of 120 genes in each locus was investigated (Fig....”
Q39XG6 Pyruvate carboxylase from Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
45% identity, 6% coverage
gll0958 probable urea amidolyase from Gloeobacter violaceus PCC 7421
39% identity, 6% coverage
Q5HQ53 Pyruvate carboxylase from Staphylococcus epidermidis (strain ATCC 35984 / DSM 28319 / BCRC 17069 / CCUG 31568 / BM 3577 / RP62A)
38% identity, 6% coverage
SE0813 pyruvate carboxylase from Staphylococcus epidermidis ATCC 12228
38% identity, 6% coverage
SM_b21124 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) from Sinorhizobium meliloti 1021
SMb21124 putative methylcrotonoyl-CoA carboxylase biotinylated subunit protein from Sinorhizobium meliloti 1021
44% identity, 9% coverage
CAETHG_1594 pyruvate carboxylase from Clostridium autoethanogenum DSM 10061
42% identity, 5% coverage
- Clostridium autoethanogenum alters cofactor synthesis, redox metabolism, and lysine-acetylation in response to elevated H2:CO feedstock ratios for enhancing carbon capture efficiency
Davin, Biotechnology for biofuels and bioproducts 2024 - “...key proteins, the biotin-carrier component of acetyl-CoA carboxylase complex (ACC, CAETHG_0127) and pyruvate carboxylase (PYC, CAETHG_1594), are regulated post-translationally by BirA biotinylation in the related bacterium, C. ljungdahlii [ 26 ]. This report prompted an examination of biotinylation in C. autoethanogenum . The search for biotinylation...”
- Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia
Brown, Biotechnology for biofuels 2014 - “...dikinase (PPDK) CAETHG_2055, 2909, P 3 Pyruvate kinase (Pk) CAETHG_2440-41, P 4 Pyruvate carboxylase (Pyc) CAETHG_1594, P 5 PEP carboxykinase (PEPCK) CAETHG_2721, P 5 Malic enzyme CAETHG_0605, 1055. Incomplete TCA cycle. T 1 Citrate synthase CAETHG _2751, T 2 Citrate lyase CAETHG_1052-54, 18981901, 2480-83, T 3...”
D0N1Q9 Methylcrotonoyl-CoA carboxylase subunit alpha, putative from Phytophthora infestans (strain T30-4)
47% identity, 9% coverage
- Cinnamaldehyde inhibits the growth of Phytophthora capsici through disturbing metabolic homoeostasis
Wang, PeerJ 2021 - “...Down/0.48 H3GJ13 1,3-beta-glucanosyltransferase Down/0.81 H6U2P7 Cellulose synthase 3 Down/0.70 Leucine metabolism A0A0W8CX17 Isovaleryl-CoA dehydrogenase Down/0.63 D0N1Q9 Methylcrotonoyl-CoA carboxylase 1 Down/0.77 Discussion There are few reports on the mechanism of action of CA on fungi and oomycetes. OuYang et al. (2019) found that after CA treatment, the...”
- “...metabolic W2NUF9 CAMK/CAMK1 protein kinase Down/0.319 D0NCV1 Glucan 1,3-beta-glucosidase Down/0.408 H3GJ13 1,3-beta-glucanosyltransferase Down/0.491 Leucine metabolism D0N1Q9 Methylcrotonoyl-CoA carboxylase subunit alpha, putative Down/0.453 A0A0W8CX17 Isovaleryl-CoA dehydrogenase Down/0.345 Internal reference protein A0A0W8DRZ2 40S ribosomal protein S13 Q2M3Z6 Ribosomal protein L23 10.7717/peerj.11339/fig-8 Figure 8 Relative expression of mRNAs from...”
CLJU_c37390 pyruvate carboxylase from Clostridium ljungdahlii DSM 13528
42% identity, 5% coverage
- Pleiotropic Regulator GssR Positively Regulates Autotrophic Growth of Gas-Fermenting Clostridium ljungdahlii
Zhang, Microorganisms 2023 - “...1 , No. 615) to examine phenotypic outcomes. Among these candidates, only the overexpression of CLJU_c37390 significantly affected the autotrophic growth of C. ljungdahlii ( Figure 4 A), while the others had no influence on cell growth ( Supplementary Figure S2 ). According to the genome...”
- “...38 ]. Based on this finding, we further examined the interaction between GssR and the CLJU_c37390 gene using EMSA. Specifically, a 305 bp DNA fragment covering the promoter region of the CLJU_c37390 gene was used as the DNA probe. As shown in Figure 4 B, GssR...”
- Functional dissection and modulation of the BirA protein for improved autotrophic growth of gas-fermenting Clostridium ljungdahlii
Zhang, Microbial biotechnology 2021 - “...Nterminal DNAbinding domain of Cl BirA) (Fig. 8A ); simultaneously, the acc (Clju_c4210042140) and pyc (Clju_c37390) genes, coding for the C. ljungdahlii ACC and PYC enzymes, respectively, were also overexpressed separately (Fig. 8A ). Such an engineering strategy was expected to specifically block the regulatory function,...”
- Clostridium ljungdahlii represents a microbial production platform based on syngas
Köpke, Proceedings of the National Academy of Sciences of the United States of America 2010 - “...and C4 compounds: pyruvate carboxylase (encoded by CLJU_c37390) and PEP-carboxykinase (encoded by CLJU_06210). C. ljungdahlii operates a branched TCA "cycle,"...”
PYCB_METJA / Q58628 Pyruvate carboxylase subunit B; Pyruvic carboxylase B; EC 6.4.1.1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
42% identity, 11% coverage
- function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
catalytic activity: hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+) (RHEA:20844)
cofactor: Mg(2+) Mn(2+) Co(2+)
subunit: Heterooctamer of four A and four B subunits
liuD / Q9I299 methylcrotonyl-CoA carboxylase α-subunit (EC 6.4.1.4) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
Q9I299 methylcrotonoyl-CoA carboxylase (subunit 2/2) (EC 6.4.1.4) from Pseudomonas aeruginosa (see 3 papers)
PA2012 alpha subunit of geranoyl-CoA carboxylase, GnyA from Pseudomonas aeruginosa PAO1
39% identity, 11% coverage
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - 3-methylcrotonyl-CoA carboxylase deficiency: clinical, biochemical, enzymatic and molecular studies in 88 individuals
Grünert, Orphanet journal of rare diseases 2012 - “...human MCCC1 (hMCCC1, Uniprot Q96RQ3), as well as the structurally characterized P. aeruginosa MCCC1 (paMCCC1, Q9I299), Ruegeria pomeroyi PCC (bPCCA, Q5LUF3), human ACC1 (hACC1, Q13085) and ACC2 (hACC2, O00763). Novel MCCC1 missense mutations are asterisked. The electrostatic interaction between Glu288 and Arg444 in MCCC1 is highlighted...”
- The opportunistic pathogen Pseudomonas aeruginosa exploits bacterial biotin synthesis pathway to benefit its infectivity
Shi, PLoS pathogens 2023 - “...aeruginosa . Genome-wide mining of P . aeruginosa reveals five putative biotinylated enzymes, namely PA1400, PA2012, PA2891, PA4847, and PA5435 ( S1 Table ). D. Streptavidin blot analyses for crude extracts of E . coli and P . aeruginosa . Unlike E . coli displaying a...”
- “...the presence of multiple biotinylated proteins. E. SDS-PAGE (12%) profile for three biotinylated enzymes (PA1400, PA2012, and PA2891). Three of five predictive biotinylated enzymes were overexpressed, purified, and judged with SDS-PAGE (12%). F. Western blot analysis of the three recombinant proteins (PA1400, PA2012, and PA2891) using...”
- Small Colony Variants and Single Nucleotide Variations in Pf1 Region of PB1 Phage-Resistant Pseudomonas aeruginosa
Lim, Frontiers in microbiology 2016 - “...9.E-04 PA2000 Dehydrocarnitine CoA transferase, subunit B -1.6 5.E-02 PA2001 atoB Acetyl-CoA acetyltransferase -1.9 1.E-02 PA2012 gnyA Alpha subunit of geranoyl-CoA carboxylase -2.6 4.E-02 PA2013 gnyH Gamma-carboxygeranoyl-CoA hydratase -2.6 2.E-02 PA2014 gnyB Beta subunit of geranoyl-CoA carboxylase -2.3 2.E-02 PA2247 bkdA1 2-oxoisovalerate dehydrogenase (alpha subunit) -2.7...”
- The Pseudomonas aeruginosa Isohexenyl Glutaconyl Coenzyme A Hydratase (AtuE) Is Upregulated in Citronellate-Grown Cells and Belongs to the Crotonase Family
Poudel, Applied and environmental microbiology 2015 - “...of California, Berkeley PA1535 PA1982 PA1984 PA2011 PA2012 PA2013 PA2014 PA2015 PA2886 PA2887 PA2888 PA2889 PA2890 PA2891 PA2892 PA4330 Gene Structure-Function...”
- Pseudomonas aeruginosa twitching motility-mediated chemotaxis towards phospholipids and fatty acids: specificity and metabolic requirements
Miller, Journal of bacteriology 2008 - “...PA0796 PA0797 PA0887 PA1137 PA1288 PA1736 PA1737 PA2011 PA2012 PA2013 PA2014 PA2015 PA2142 PA2552 PA2553 PA2554 PA2555 PA2557 PA2634 PA2764 PA2862 PA2863 PA2887...”
- The atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine in Pseudomonas aeruginosa
Aguilar, Applied and environmental microbiology 2006 - “...PA2893 PAO1::ISlacZ/hah (ID 11254), transposon insertion in ORF PA2012 PAO1SM mutant obtained by Himar1::Gmr transposition in ORF PA2891, unable to grow on...”
- “...PAO1SM mutant obtained by Himar1::Gmr transposition in ORF PA2012, unable to grow on citronellol Double mutant strain derived from PAO atuF, mutated in liuD...”
- Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa
Höschle, Microbiology (Reading, England) 2005 (PubMed)- “...unequivocally identified as the gene products of liuD (PA2012; 74 kDa) and atuF (PA2891; 71 kDa), respectively (Figs 2 and 3). The three non-biotin-containing...”
- Effect of anaerobiosis and nitrate on gene expression in Pseudomonas aeruginosa
Filiatrault, Infection and immunity 2005 - “...PA1556 PA1561 PA1673 PA1789 PA1806 PA1882 PA1883 PA1939 PA2012 PA2013 PA2014 PA2015 PA2016 PA2024 PA2026 PA2073 PA2110 PA2112 PA2113 hemF aroE 4.5 3.5 5.1...”
- “...PA1986 PA1987 PA1988 PA1989 PA1990 PA1999 PA2003 PA2011 PA2012 PA2013 PA2014 PA2024 PA2052 PA2066 PA2067 PA2068 PA2069 PA2110 PA2112 PA2114 PA2194 PA2250 PA2266...”
- The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa
Díaz-Pérez, Applied and environmental microbiology 2004 - “...showed that transposon insertion had occurred in the PA2012 and PA2014 ORFs, respectively, from the PGP database (26). Identification of the gny cluster from...”
- “...gnyB, gnyA, and gnyL genes (ORFs PA2015, PA2014, PA2012, and PA2011, respectively) were constructed as described in Materials and Methods. PCR analysis of...”
PA14_38480 alpha subunit of geranoyl-CoA carboxylase, GnyA from Pseudomonas aeruginosa UCBPP-PA14
39% identity, 11% coverage
APA386B_218 5-oxoprolinase/urea amidolyase family protein from Acetobacter pasteurianus 386B
35% identity, 6% coverage
EUBREC_1628 pyruvate carboxylase subunit B from Eubacterium rectale ATCC 33656
42% identity, 52% coverage
CC77DRAFT_1020016 pyruvate carboxylase from Alternaria alternata
44% identity, 6% coverage
Caur_2832 Carbamoyl-phosphate synthase L chain ATP-binding from Chloroflexus aurantiacus J-10-fl
39% identity, 9% coverage
SPA0056 oxaloacetate decarboxylase alpha chain from Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150
40% identity, 11% coverage
NCgl0670 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Corynebacterium glutamicum ATCC 13032
cg0802 biotin carboxylase and biotin carboxyl carrier protein from Corynebacterium glutamicum ATCC 13032
42% identity, 11% coverage
- Identification of key genes through the constructed CRISPR-dcas9 to facilitate the efficient production of O-acetylhomoserine in Corynebacterium glutamicum
Li, Frontiers in bioengineering and biotechnology 2022 - “...of pyruvate catabolism; ackA , pta , NCgl1987 of acetate acylation; alaA , NCgl2247 , NCgl0670 , NCgl2309 , NCgl0245 of acetyl-CoA utilization and purA , argG of l -aspartic acid degradation and metabolism. To identify the relationship between these candidate genes and OAH biosynthesis, N20...”
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics
Zhang, Scientific reports 2018 - “...0.00 4006.09 8.3 53 NCgl2709 cg3107 adhA Zn-dependent alcohol dehydrogenase 5.23/36811.85 5992.18 0.00 66.7 61 NCgl0670 cg0802 accBC Biotin carboxylase and carboxyl carrier protein 5.02/63419.65 710.24 0.00 5.1 63 NCgl2698 cg3096 ald Aldehyde dehydrogenase 4.96/55106.23 1004.77 0.00 24.0 64 NCgl0967 cg1145 fumC Fumarate hydratase 5.06/49763.37 14985.21...”
- In Vivo Roles of Fatty Acid Biosynthesis Enzymes in Biosynthesis of Biotin and α-Lipoic Acid in Corynebacterium glutamicum
Ikeda, Applied and environmental microbiology 2017 - “...and pCaccD1 were constructed so that accBC (Cgl0700, NCgl0670) and accD1 (Cgl0708, NCgl0678) were constitutively expressed under the promoter of the endogenous...”
- Altered acetylation and succinylation profiles in Corynebacterium glutamicum in response to conditions inducing glutamate overproduction
Mizuno, MicrobiologyOpen 2016 - “...the most drastic change in protein abundance (34.1fold decrease in the glutamate producing condition). AccBC (NCgl0670) and DtsR2 (NCgl0677), the other subunits of acetylCoA carboxylase, were 2.4fold and 1.2fold less abundant in the glutamateproducing condition, respectively. These results were consistent with the transcriptome analysis reporting severe...”
- Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine
Lange, Applied and environmental microbiology 2003 - “...2624 2629 NCgl2582 NCgl2621 NCgl0099 NCgl0469 NCgl0634 NCgl0670 NCgl0754 NCgl1900 NCgl1961 NCgl1512 NCgl2268 NCgl0627; NCgl0628 NCgl1345 NCgl1340 Identical to...”
- Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids
Chu, Molecules (Basel, Switzerland) 2024 - “...the carboxyltransferase partial reaction [ 60 ]. In recent years, episomal overexpression of accBC ( cg0802 ) and accD1 ( cg0812 ) was replaced by the TetR-type transcriptional repressor FasR ; it effectively increases malonyl-CoA availability and accumulation of the flavonoid naringenin. FasR not only repressed...”
- “...also the deletion of FasR lead to the deregulation of the expression of accBC ( cg0802 ) and accD1 ( cg0812 ). Therefore, FasR has a significant role in improving malonyl-CoA availability in cytosol of C. glutamicum ( Figure 5 ) [ 56 ]. Glucose was...”
- Functional Genomics Uncovers Pleiotropic Role of Rhomboids in Corynebacterium glutamicum
Luenenschloss, Frontiers in microbiology 2022 - “...are in line with the earlier assumed peptidoglycan remodeling. Lipid Transport and Metabolism Acetyl/propionyl-CoA carboxylase (Cg0802) and Acetyl-CoA carboxylase (Cg0812) decreased in the MF, whereas 3-oxoacyl-[acyl-carrier protein] reductase Fas-IB (Cg0957) increased in the MF, but decreased in the CF at 30 and 40C, yet 3-oxoacyl-[acyl-carrier protein]...”
- “...of two fatty acids to mycolic acid, respectively. The former consists of the subunits AccbC (Cg0802) and AccD1 (Cg0812), the latter of AccbC (Cg0802), AccD2 (Cg0811), AccD3 (Cg3177), and AccE (Cg0810) ( Gande et al., 2007 ). The malonyl-CoA is utilized for fatty acid synthesis with...”
- Tailoring Corynebacterium glutamicum towards increased malonyl-CoA availability for efficient synthesis of the plant pentaketide noreugenin
Milke, Microbial cell factories 2019 - “...reduction of the flux into the tricarboxylic acid (TCA) cycle, whereas episomal overexpression of accBC (cg0802) and accD1 (cg0812) encoding the two subunits of the acetyl-CoA carboxylase complex (ACC)hardly increased product titers. In C. glutamicum , transcription of accBC and accD1 is controlled by the transcriptional...”
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics
Zhang, Scientific reports 2018 - “...4006.09 8.3 53 NCgl2709 cg3107 adhA Zn-dependent alcohol dehydrogenase 5.23/36811.85 5992.18 0.00 66.7 61 NCgl0670 cg0802 accBC Biotin carboxylase and carboxyl carrier protein 5.02/63419.65 710.24 0.00 5.1 63 NCgl2698 cg3096 ald Aldehyde dehydrogenase 4.96/55106.23 1004.77 0.00 24.0 64 NCgl0967 cg1145 fumC Fumarate hydratase 5.06/49763.37 14985.21 8104.40...”
5ks8F / Q1H157 Crystal structure of two-subunit pyruvate carboxylase from methylobacillus flagellatus (see paper)
39% identity, 14% coverage
- Ligand: manganese (ii) ion (5ks8F)
Q5GSI1 Propionyl-CoA carboxylase alpha chain, mitochondrial from Wolbachia sp. subsp. Brugia malayi (strain TRS)
45% identity, 9% coverage
5ks8D / Q1H157 Crystal structure of two-subunit pyruvate carboxylase from methylobacillus flagellatus (see paper)
39% identity, 11% coverage
- Ligands: 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal; pyruvic acid; manganese (ii) ion (5ks8D)
CA51_17250 pyruvate carboxylase from Rosistilla oblonga
37% identity, 6% coverage
Mal33_20070 pyruvate carboxylase from Rosistilla oblonga
37% identity, 6% coverage
PFJ30894_RS04445 biotin/lipoyl-containing protein from Phascolarctobacterium faecium
40% identity, 50% coverage
F1KUZ6 Propionyl-CoA carboxylase alpha chain, mitochondrial from Ascaris suum
44% identity, 8% coverage
WP_000809507 pyruvate carboxylase from Staphylococcus aureus
36% identity, 6% coverage
DEFDS_1275 pyruvate carboxylase from Deferribacter desulfuricans SSM1
40% identity, 6% coverage
NWMN_0979 pyruvate carboxylase from Staphylococcus aureus subsp. aureus str. Newman
36% identity, 6% coverage
- Comparative Transcriptome Analysis Reveals Differentially Expressed Genes Related to Antimicrobial Properties of Lysostaphin in Staphylococcus aureus
Yan, Antibiotics (Basel, Switzerland) 2022 - “...Newman_WT. Of the 21 significant SNPs, 18 were associated with 4 individual genes (NWMN_0305, NWMN_0306, NWMN_0979, and NWMN_1288). The remaining three SNPs were distributed in the intergenic regions: two of them were between the lctp and Spa genes and one was located between the recR and...”
- “...region (between recR and tmk ) WGS mutant contigs 1,086,625 AGC AGT S S Yes NWMN_0979 ( pycA ): pyruvate carboxylase 1,085,501 1,088,971 WGS mutant reads 1,086,625 AGC AGT S S Yes NWMN_0979 ( pycA ): pyruvate carboxylase 1,085,501 1,088,971 RNA-seq mutant contig 1,086,625 AGC AGT...”
- CodY in Staphylococcus aureus: a regulatory link between metabolism and virulence gene expression
Pohl, Journal of bacteriology 2009 - “...NWMN_0379 NWMN_1110 NWMN_1111 NWMN_1112 NWMN_1249 NWMN_0163 NWMN_0162 NWMN_0979 NWMN_1325 NWMN_2294 NWMN_0167 Gene Newman vs codY mutant agr mutant vs agr codY...”
D11S_1380 oxaloacetate decarboxylase alpha subunit from Aggregatibacter actinomycetemcomitans D11S-1
41% identity, 11% coverage
D9Q935 Biotin/lipoyl-binding protein from Corynebacterium pseudotuberculosis (strain C231)
CpC231_0574 biotin/lipoyl-containing protein from Corynebacterium pseudotuberculosis C231
40% identity, 52% coverage
- Changes in protein abundance are observed in bacterial isolates from a natural host
Rees, Frontiers in cellular and infection microbiology 2015 - “...* Lipid transport and metabolism D9QEV7 CpC231_0543 CpC231_0543 Uncharacterized protein 0.923 * 1.119 * 0.183 D9Q935 CpC231_0574 CpC231_0574 Methylmalonyl-CoA carboxyltransferase 1.3S subunit 1.088 * 0.381 0.228 Lipid transport and metabolism D9Q954 rpfA CpC231_0595 Resuscitation-promoting factor 0.724 0.020 1.458 * D9Q998 pcrA CpC231_0641 DNA helicase 0.070 1.359...”
- Changes in protein abundance are observed in bacterial isolates from a natural host
Rees, Frontiers in cellular and infection microbiology 2015 - “...Lipid transport and metabolism D9QEV7 CpC231_0543 CpC231_0543 Uncharacterized protein 0.923 * 1.119 * 0.183 D9Q935 CpC231_0574 CpC231_0574 Methylmalonyl-CoA carboxyltransferase 1.3S subunit 1.088 * 0.381 0.228 Lipid transport and metabolism D9Q954 rpfA CpC231_0595 Resuscitation-promoting factor 0.724 0.020 1.458 * D9Q998 pcrA CpC231_0641 DNA helicase 0.070 1.359 *...”
QV15_05170 pyruvate carboxylase from Staphylococcus aureus
SAR1088 putative pyruvate carboxylase from Staphylococcus aureus subsp. aureus MRSA252
36% identity, 6% coverage
- A spectrum of CodY activities drives metabolic reorganization and virulence gene expression in Staphylococcus aureus
Waters, Molecular microbiology 2016 - “...transporters for oligopeptides (QV15_04450-04470), p -aminobenzoyl-glutamate (QV15_12780), and galactitol (QV15_00960) are turned on early. pycA (QV15_05170), sucA (QV15_06885) and QV15_00370, coding for pyruvate carboxylase, 2-oxoglutarate dehydrogenase and acetoin reductase - all of which control the distribution of pyruvate and 2-oxoglutarate - are also turned on early....”
- Staphylococcus aureus mutant screen reveals interaction of the human antimicrobial peptide dermcidin with membrane phospholipids
Li, Antimicrobial agents and chemotherapy 2009 - “...10-14 6-15 8-16 38-40 6-14 10-16 1.6 1.5 SAR1343 SAR0674 SAR1088 SAR1196 SAR1202 SAR1075 1 6 3 3 1 4 20-23 8-16 9-12 8-14 52-54 8-14 1.4 Bioluminescence assay...”
- The Staphylococcus aureus response to unsaturated long chain free fatty acids: survival mechanisms and virulence implications
Kenny, PloS one 2009 - “...SAR0753 fruA fructose-specific PTS system component 2.50 3.78E-02 SAR0766 glutamine amidotransferase class-I protein 2.04 6.16E-04 SAR1088 putative pyruvate carboxylase 2.5 8.78E-04 SAR1450 tdcB putative threonine dehydratase 2.22 5.47E-03 SAR1451 ald2 alanine dehydrogenase 2 3.03 1.15E-03 SAR1777 pfkA 6-phosphofructokinase 2.86 2.87E-03 SAR1789 ackA acetate kinase 2.33 9.23E-03...”
- “...pyruvate levels. Concomitantly, there was down-regulation of genes involved in pyruvate utilisation, including ldh1 , SAR1088 ( pycA ), ald2 and SAR0355 converting pyruvate to lactate, oxaloacetate, alanine and cysteine, respectively. Reduced transcription of SAR2143 ( ilvC ) could further lower the expenditure of cellular pyruvate...”
PYC_STAAM / A0A0H3JRU9 Pyruvate carboxylase; EC 6.4.1.1 from Staphylococcus aureus (strain Mu50 / ATCC 700699) (see 3 papers)
A0A0H3JRU9 pyruvate carboxylase (EC 6.4.1.1) from Staphylococcus aureus (see 4 papers)
NP_371638 pyruvate carboxylase from Staphylococcus aureus subsp. aureus Mu50
SA0963 pyruvate carboxylase from Staphylococcus aureus subsp. aureus N315
BJL64_05525, SAV1114 pyruvate carboxylase from Staphylococcus aureus
36% identity, 6% coverage
- function: Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
catalytic activity: hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+) (RHEA:20844)
cofactor: biotin
subunit: Homotetramer. - Blood Serum Affects Polysaccharide Production and Surface Protein Expression in S. Aureus
Islam, Advances in biotechnology & microbiology (Newbury, Calif.) 2017 - “...RNA polymerase(rpoB) Transcription of DNA into RNA 6_5/135 NP_371067 2.Pyruvate carboxylase (pycA) Carbohydrate metabolism 5_0/128 NP_371638 3.1soleucyl-tRNA synthetase (ileS) Aminio Acid biosynthesis 5.2/104 NP_371717 b6 1. Alanyl-tRNA synthetase RNA synthesis 4.9/93 NP_372142 2. Hypothetical protein (SAV0219) Not known 4.6/37 NP_370743 3.Collagen adhesin precursor (MW2612) Surface Adhesin...”
- Respiration and Small Colony Variants of Staphylococcus aureus
Proctor, Microbiology spectrum 2019 (secret) - Novel single-nucleotide variations associated with vancomycin resistance in vancomycin-intermediate Staphylococcus aureus
Lin, Infection and drug resistance 2018 - “...Transcriptome analysis data 10 SA1659 prsA 18927181893680 Peptidyl-prolyl cis/trans isomerase homolog Deletion frameshift mutation 10 SA0963 pycA 10912421094694 Pyruvate carboxylase Transcriptome analysis data 10 SA0500 rpoB 579620583171 RNA polymerase beta chain SNP (G171D, A447V, D471Y, H481Y) 4 SA1872 rsbU complement (21198212120822) SigmaB regulation protein RsbU Transcriptome...”
- Exposure of clinical MRSA heterogeneous strains to β-lactams redirects metabolism to optimize energy production through the TCA cycle
Keaton, PloS one 2013 - “...sucD 3.3 succinyl-CoA synthetase alpha subunit Catalyzes the only substrate-level phosphorylation in the TCA cycle. SA0963 pycA 3 pyruvate carboxylase Involved in the TCA cycle, alanine and aspartate metabolism, pyruvate metabolism. SA1510 gapB 4.8 glyceraldehyde 3-phosphate dehydrogenase Involved in glycolysis and glyconeogenesis. SA1845 2.2 hypothetical protein...”
- “...Genes encoding enzymes involved in gluconeogenesis were similarly down-regulated including pyruvate carboxylase ( pycA , SA0963), which catalyzes the conversion of pyruvate into oxaloacetate, and fructose-1,6-bisphosphatase III (SA2401). The only glycolytic gene observed to be up-regulated during the HeR/HoR selection was glyceraldehyde-3-phosphate dehydrogenase ( gapB ,...”
- Transcriptional profiling analysis of the global regulator NorG, a GntR-like protein of Staphylococcus aureus
Truong-Bolduc, Journal of bacteriology 2011 - “...SA1916 SA0994 SA0995 SA1110 SA2410 SA2411 SA2412 SA0964 SA0963 SA1158 SA1159 SA1160 SA1478 SA2198 SA2617 SA2199 SA1714 SA1715 Amino acid ABC transporter Amino...”
- walK and clpP mutations confer reduced vancomycin susceptibility in Staphylococcus aureus
Shoji, Antimicrobial agents and chemotherapy 2011 - “...0.43 0.37 0.35 0.35 0.34 Succinate dehydrogenase SA0963 SA0994 SA0995 SA0996 pycA sdhC sdhA sdhB Pyruvate carboxylase Succinate dehydrogenase, cytochrome b-558...”
- Staphylococcal major autolysin (Atl) is involved in excretion of cytoplasmic proteins
Pasztor, The Journal of biological chemistry 2010 - “...5 4.9 8.3 4.6 5.2 4.9 6.5 5.5 4.7 4.9 SA0935 SA0963 SA0500 SA1099 SA0829 SAS074 SA0719 01029 01064 00524 01232 00906 02425 00785 63 129 133 29.1 33.1 10 34 4.6...”
- Large-scale identification of genes required for full virulence of Staphylococcus aureus
Benton, Journal of bacteriology 2004 - “...asd 428 1,073 425 329 154 589 131 91 SA1749 SA0963 SA1044 SA1226 IVT35 vga 642 512 SA1852 IVT133 yflS (B. subtilis) 472 159 SA2486 IVT176 IVT207 yuiF (B....”
- Borosilicate bioactive glass synergizing low-dose antibiotic loaded implants to combat bacteria through ATP disruption and oxidative stress to sequentially achieve osseointegration
Fan, Bioactive materials 2025 - “...on cell membranes [ 47 , 48 ]. In our results, SACOL1102 , SAV1094 , SAV1114 , BSU28450 , etc., relative to genes involved in the TCA cycle, were significantly downregulated in S. aureus in the B/G/PMMA group. The expression of NADP binding-related genes (such as...”
- Joint Genomic and Proteomic Analysis Identifies Meta-Trait Characteristics of Virulent and Non-virulent Staphylococcus aureus Strains
Bonar, Frontiers in cellular and infection microbiology 2018 - “...protein (lipoprotein) BJL64_05335 [BJL65_05325] 69 Internal stop codon in ch22. 6. 975 Pyruvate carboxylase PycA BJL64_05525 [BJL65_05515] 1,150 Internal stop codon in ch22. 7. 1017 Hypothetical protein BJL64_05735 [BJL65_05725] 55 Internal stop codon in ch22. 8. 1702 Exotoxin SeN BJL64_09410 [BJL65_10575] 251 Internal stop codon in...”
KMZ21_05015 pyruvate carboxylase from Staphylococcus aureus subsp. aureus RN4220
SAOUHSC_01064 pyruvate carboxylase from Staphylococcus aureus subsp. aureus NCTC 8325
SACOL1123 pyruvate carboxylase from Staphylococcus aureus subsp. aureus COL
YP_499610 pyruvate carboxylase from Staphylococcus aureus subsp. aureus NCTC 8325
36% identity, 6% coverage
- Hybrid assembly using long reads resolves repeats and completes the genome sequence of a laboratory strain of Staphylococcus aureus subsp. aureus RN4220
Panthee, Heliyon 2022 - “...- 15 KMZ21_04460 461 mgtE 925408..926793 - 16 KMZ21_04700 36 hypothetical protein 972806..972916 - 17 KMZ21_05015 1150 pyruvate carboxylase 1037702..1041154 - 18 KMZ21_05785 567 proline tRNA ligase 1196448..1198151 - 19 KMZ21_06475 97 transposase 1341780..1342073 - 20 KMZ21_06560 160 transposase 1357791..1358271(-) - 21 KMZ21_06705 9535 ebh: hyperosmolarity...”
- Proteomic and Metabolomic Analyses of a Tea-Tree Oil-Selected Staphylococcus aureus Small Colony Variant
Torres, Antibiotics (Basel, Switzerland) 2019 - “...metabolism 1.5 SAOUHSC_01043 PdhD dihydrolipoamide dehydrogenase carbohydrate metabolism 1.3 SAOUHSC_01058 TypA GTP-binding protein unclassified 1.7 SAOUHSC_01064 PycA pyruvate carboxylase carbohydrate metabolism 1.7 SAOUHSC_01091 SpoU tRNA G18 (ribose-2-O)-methylase uncharacterized 1.4 SAOUHSC_01093 PheT phenylalanyl-tRNA synthetase subunit beta protein synthesis 1.4 SAOUHSC_01110 Ecb extracellular complement-binding protein virulence factor 4.3...”
- Copper stress in Staphylococcus aureus leads to adaptive changes in central carbon metabolism
Tarrant, Metallomics : integrated biometal science 2019 - “...2.1 <0.00010 d -Alanine-poly(phosphoribitol) ligase subunit 1 dltA SAOUHSC_00869 gi|122539917 2 <0.00010 Pyruvate carboxylase pycA SAOUHSC_01064 gi|88194813 1.3 0.00013 Histone-like DNA-binding protein HU hup SAOUHSC_01490 gi|446966607 1.2 <0.00010 Proteins with decreased abundance in cells cultured in 1 mM Cu in TM medium Cold-shock protein cspC SAOUHSC_00819...”
- “...pdhC SAOUHSC_01042 gi|88194794 0.6 <0.00010 Bifunctional autolysin atl SAOUHSC_00994 gi|110832765 0.6 0.00038 Pyruvate carboxylase pycA SAOUHSC_01064 gi|88194813 0.6 <0.00010 Phosphoribosylamine-glycine ligase purD SAOUHSC_01018 gi|88194772 0.6 0.00074 DNA-directed RNA polymerase subunit beta rpoC SAOUHSC_00525 gi|114152142 0.5 0.00013 Elongation factor Tu tuf SAOUHSC_00530 gi|123098060 0.2 <0.00010 Alkaline shock...”
- A multihost bacterial pathogen overcomes continuous population bottlenecks to adapt to new host species
Bacigalupe, Science advances 2019 - “...NCTC8325 SNP 813,228 Missense metN2 Methionine import ATP-binding protein MetN2 TM NCTC8325 SNP 1,030,094 Missense SAOUHSC_01064 Pyruvate carboxylase TM NCTC8325 SNP 1,067,459 Missense sdhB Iron-sulfur subunit of succinate dehydrogenase%2C putative TM NCTC8325 SNP 1,121,026 Missense carB Carbamoyl phosphate synthase large subunit TM NCTC8325 SNP 1,138,999 Missense...”
- Protein CoAlation and antioxidant function of coenzyme A in prokaryotic cells
Tsuchiya, The Biochemical journal 2018 - “...LYGFC*IQR 106.68 pth Peptidyl-tRNA hydrolase 21.703 C*IVGLGNIGK 84.31 dnaK Chaperone protein DnaK 66.361 IIGIDLGTTNSC*VTVLEGDEPK 89.507 SAOUHSC_01064 Pyruvate carboxylase 18.812 C*AEEGIK18.812 77.73 sarR HTH-type transcriptional regulator SarR 13.669 C*SEFKPYYLTK 98.421 lepA Elongation factor 4 28.674 C*YGGDISR 128.35 asnS AsparaginetRNA ligase 49.157 SVLENC*KLELK 125.97 pfkA ATP-dependent 6-phosphofructokinase 34.839...”
- Bacterial Hypoxic Responses Revealed as Critical Determinants of the Host-Pathogen Outcome by TnSeq Analysis of Staphylococcus aureus Invasive Infection
Wilde, PLoS pathogens 2015 - “...that feed into the TCA cycle were also important for intraosseous growth, including pyruvate carboxylase (SAOUHSC_01064 pyc ), pyruvate dehydrogenase (SAOUHSC_01040 pdhA ), and a putative malic enzyme (SAOUHSC_01810). Mutations in 7 S . aureus genes encoding amino acid biosynthesis enzymes compromised bacterial growth during osteomyelitis,...”
- Genes contributing to Staphylococcus aureus fitness in abscess- and infection-related ecologies
Valentino, mBio 2014 - “...play an important role in Francisella virulence ( 52 ). Additionally, mutants in pyruvate carboxylase (SAOUHSC_01064) and aspartate aminotransferase (SAOUHSC_02158), involved in production of aspartate from pyruvate ( 53 ), were observed to be less abundant under all infection conditions tested, indicating a critical role for...”
- Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
Pagels, Molecular microbiology 2010 - “...0.8 (0.8) 3.4 (1.4) 0.5 (0.5) 6.6 (3.1) 1.6 (1.7) SACOL0976 Hydrolase, haloacid dehalogenase-like family SAOUHSC_01064 Pyc 2 1.4 (11.4) 1.7 (1.4) 5.3 (6.8) 0.3 (0.2) 0.3 (1.7) SACOL1123 Pyruvate carboxylase SAOUHSC_01218 SucD 1.4 (1.1) 3.8 (1.5) 1.6 (1.2) 2.4 (1.3) 0.9 (0.9) SACOL1263 Succinyl-CoA synthetase...”
- Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
Pagels, Molecular microbiology 2010 - “...dehalogenase-like family SAOUHSC_01064 Pyc 2 1.4 (11.4) 1.7 (1.4) 5.3 (6.8) 0.3 (0.2) 0.3 (1.7) SACOL1123 Pyruvate carboxylase SAOUHSC_01218 SucD 1.4 (1.1) 3.8 (1.5) 1.6 (1.2) 2.4 (1.3) 0.9 (0.9) SACOL1263 Succinyl-CoA synthetase subunit alpha SAOUHSC_01266 1.3 (1.2) 5.5 (4.4) 6.5 (5.2) 0.8 (0.8) 0.2 (0.2)...”
- Allosteric Site at the Biotin Carboxylase Dimer Interface Mediates Activation and Inhibition in Staphylococcus aureus Pyruvate Carboxylase.
Laseke, Biochemistry 2023 - GeneRIF: Allosteric Site at the Biotin Carboxylase Dimer Interface Mediates Activation and Inhibition in Staphylococcus aureus Pyruvate Carboxylase.
SAUSA300_1014 pyruvate carboxylase from Staphylococcus aureus subsp. aureus USA300_FPR3757
36% identity, 6% coverage
- Growth and Stress Tolerance Comprise Independent Metabolic Strategies Critical for Staphylococcus aureus Infection
Kim, mBio 2021 - “...17791 1013 + + + + SAUSA300_2073 tdk 5015 5338 992 + + + + SAUSA300_1014 pyc 582 12356 1001 + + + + 2,675 1,136 *** 325,000 208,601 *** SAUSA300_0959 fmtA 469 229 1002 + + + + SAUSA300_1112 stp1 6522 189 983 + +...”
- “...108.524.61 119.6325.29 35.076.38 0.60.21 SAUSA300_1246 acnA 4.8 1.020.04 2.51 77.0834.94 * 85.6320.98 29.829.97 0.760.31 * SAUSA300_1014 pyc 5.54 0.830.04 **** 2.85 66.5318.67 **** 145.2136.90 * 24.096.32 *** 0.640.32 SAUSA300_1902 PPP 5.98 0.940.12 *** 3.84 40.5811.60 **** 55.6121.59 *** 233.24 * 0.830.13 ** SAUSA300_0961 qoxC Oxphos 11.25...”
SPy1183 putative decarboxylase, gamma chain from Streptococcus pyogenes M1 GAS
41% identity, 48% coverage
PYC_BACSU / Q9KWU4 Pyruvate carboxylase; Pyruvic carboxylase; PYC; EC 6.4.1.1 from Bacillus subtilis (strain 168) (see 2 papers)
39% identity, 6% coverage
- function: Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second, leading to oxaloacetate production (PubMed:4146915). Fulfills an anaplerotic function in B.subtilis as it is necessary for growth on glucose, but is not required for sporulation (PubMed:4146915).
catalytic activity: hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+) (RHEA:20844)
cofactor: biotin
subunit: Homotetramer (Probable). At very low potassium concentrations, when intracellular levels of c-di-AMP are low, interacts with apo-DarB (PubMed:35130724). c-di-AMP inhibits the binding of DarB to PYC (PubMed:35130724). Does not bind directly c-di-AMP (PubMed:35130724). - Proteomic Response of Bacillus subtilis Spores under High Pressure Combined with Moderate Temperature and Random Peptide Mixture LK Treatment
Pang, Foods (Basel, Switzerland) 2022 - “...pathway included 2-oxoglutarate dehydrogenase E1 component (P23129), aconitate/2-methylaconitate hydratase (P09339), malate dehydrogenase (P49814), pyruvate carboxylase (Q9KWU4), dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (A0A6A8FKK9), phosphoenolpyruvate carboxykinase (ATP) (A0A7U5BTG7), succinate-CoA ligase (ADP-forming) subunit alpha (A0A5F2KMI5), succinate dehydrogenase flavoprotein subunit (A0A3N6CX89), and succinate-CoA ligase (ADP-forming) subunit beta (A0A3A5I5U1)....”
- “...stabilizes its translation and added the accumulation of gerE protein in late-stage formation. Pyruvate carboxylase (Q9KWU4), which is in charge of catalyzing a two-step reaction, including the ATP-dependent carboxylation and the carboxyl groups transfer to pyruvate, performs an anaplerotic function in B. subtilis because it is...”
- Comprehensive analysis of temporal alterations in cellular proteome of Bacillus subtilis under curcumin treatment
Reddy, PloS one 2015 - “...E1 component subunit beta PdhB 35.47 26 -1.69 NS NS 317 171 0.015 D-9 * Q9KWU4 Pyruvate carboxylase Pyc 127.9 30 -2.75 NS NS 315 188 0.0012 D-10 O32117 NADH dehydrogenase-like protein yutJ YutJ 39.75 19 -2.28 -1.51 NI 865 737 0.014 U-1 P80244 ATP-dependent Clp...”
- A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study
Albaum, Proteome science 2011 - “...<0.000001 0.003031 325 P54537 >0.99 >0.99 <0.000001 0.004345 471 P71070 0.417433 >0.99 0.000001 0.009605 314 Q9KWU4 0.280189 >0.99 0.000448 0.009979 807 O06745 0.000005 >0.99 0.013199 0.011193 485 O35007 0.002474 >0.99 >0.99 0.011411 410 O05252 0.111360 0.104107 0.601796 0.017767 835 O32218 0.009165 >0.99 >0.99 0.018027 567 O34633...”
BSSC8_27920 pyruvate carboxylase from Bacillus subtilis subsp. subtilis str. SC-8
39% identity, 6% coverage
- Proteomic Analysis of the Characteristic Flavor Components in Bacillus subtilis BSNK-5-Fermented Soymilk
Hu, Foods (Basel, Switzerland) 2024 - “...2.20 2.47 A0A6I4DHN9 mmgD 2-methylcitrate synthase 0.49 G4ESR9 BSSC8_13990 Succinate dehydrogenase (quinone) 0.43 0.43 G4EWR2 BSSC8_27920 Pyruvate carboxylase 2.03 2.09 Pyruvate metabolism A0A199WLB6 ackA Acetate kinase 0.45 0.49 A0A6H0H0J6 acoC Acetoin dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase 0.11 0.08 A0A6H0H4J8 accC Acetyl-CoA carboxylase biotin carboxylase subunit 2.16 2.25...”
PP4067, PP_4067 acetyl-CoA carboxylase, biotin carboxylase, putative from Pseudomonas putida KT2440
45% identity, 10% coverage
Poly24_16690 pyruvate carboxylase from Rosistilla carotiformis
37% identity, 6% coverage
Sfum_0461 Conserved carboxylase region from Syntrophobacter fumaroxidans MPOB
44% identity, 9% coverage
A5G5L1 biotin carboxylase (EC 6.3.4.14) from Geotalea uraniireducens (see paper)
44% identity, 6% coverage
Pnuc_0735 pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase from Polynucleobacter sp. QLW-P1DMWA-1
51% identity, 10% coverage
AOT42_01480, DIP0649 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Corynebacterium diphtheriae bv. gravis
NP_939023 acyl coenzyme A carboxylase from Corynebacterium diphtheriae NCTC 13129
36% identity, 12% coverage
WD0433 propionyl-CoA carboxylase, alpha subunit from Wolbachia endosymbiont of Drosophila melanogaster
42% identity, 9% coverage
AO271_00235 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Corynebacterium diphtheriae
36% identity, 12% coverage
HMPREF1120_00351 pyruvate carboxylase from Exophiala dermatitidis NIH/UT8656
41% identity, 6% coverage
A1ANN6 biotin carboxylase (EC 6.3.4.14) from Pelobacter propionicus (see paper)
45% identity, 5% coverage
AO356_01595 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) from Pseudomonas fluorescens FW300-N2C3
38% identity, 9% coverage
- mutant phenotype: Specifically important for utilizing L-Leucine.
PP5346, PP_5346 oxaloacetate decarboxylase, alpha subunit from Pseudomonas putida KT2440
42% identity, 11% coverage
- New insights on the reorganization of gene transcription in Pseudomonas putida KT2440 at elevated pressure
Follonier, Microbial cell factories 2013 - “...lysine decarboxylase) -1.85 4.9 E-03 -1.69 3.9 E-03 PP_1389 carboxyphosphonoenolpyruvate phosphonomutase, putative -1.54 3.7 E-03 PP_5346 pyruvate carboxylase subunit B oadA -1.58 1.7 E-02 -1.51 1.1 E-01 PP_5347 pyruvate carboxylase subunit A accC-2 -2.23 1.9 E-03 -2.41 5.6 E-03 PP_5075 glutamate synthase subunit beta gltD +1.69...”
- Regulatory tasks of the phosphoenolpyruvate-phosphotransferase system of Pseudomonas putida in central carbon metabolism
Chavarría, mBio 2012 - “...protein (PP_4192); Fum, fumarate hydratase (PP_0897); Mdh, malate dehydrogenase (PP_0654); OadA, pyruvate carboxylase subunit B (PP_5346); AccC-2, pyruvate carboxylase subunit A (PP_5347); MaeB, malic enzyme (PP_5085); Ppc, phosphoenolpyruvate carboxylase (PP_1505). The 16 sites of the network where metabolic fluxes could be calculated as explained in the...”
- “..., two genes encode the corresponding subunits of pyruvate carboxylase: accC -2 (PP_5347) and oadA (PP_5346). To determine whether the effect of PtsN (EIIA Ntr ) on the pyruvate shunt reflects a direct or indirect action of the phosphotransferase on its constituent enzymatic activities, cell-free extracts...”
- Transcriptome dynamics of Pseudomonas putida KT2440 under water stress
Gülez, Applied and environmental microbiology 2012 - “...Annotation 4h 24 h 72 h PP_5033 PP_5338 PP_5346 hutU aspA oadA Urocanate hydratase Aspartate ammonia-lyase Oxaloacetate decarboxylase, alpha subunit 2.08 2.37...”
- Convergent peripheral pathways catalyze initial glucose catabolism in Pseudomonas putida: genomic and flux analysis
del, Journal of bacteriology 2007 - “...Other functions PP1014 PP2968 PP3384 PP3418 PP3555 PP4073 PP4488 PP5346 PP5347 a Change (n-fold). Gene gcd oprB-2 gap-1 edd glk zwf-1 eda kguD kguT kguK kguE...”
- “...corresponding gene products encode an OAA decarboxylase (PP5346; oadA), an acetyl-coenzyme A (CoA) carboxylase, and a methyl-accepting chemotaxis receptor...”
lp_2136 pyruvate carboxylase from Lactobacillus plantarum WCFS1
34% identity, 6% coverage
FGSG_07075 pyruvate carboxylase from Fusarium graminearum PH-1
35% identity, 4% coverage
FN0200 Biotin carboxyl carrier protein of glutaconyl-COA decarboxylase from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
D5RC27 Putative glutaconyl-CoA decarboxylase subunit gamma from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 23726 / VPI 4351)
Q8R5Y8 Biotin carboxyl carrier protein of glutaconyl-COA decarboxylase from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355)
42% identity, 49% coverage
- Perturbed human sub-networks by Fusobacterium nucleatum candidate virulence proteins
Zanzoni, Microbiome 2017 - “...DHP2 FN0891 Exo_endo_phos Exo_endo_phos LIG_SH2_GRB2, TRG_ENDOCYTIC_2 b Q8R5Y8 Biotin carboxyl carrier protein of glutaconyl-COA decarboxylase FN0200 Biotin_lipoyl Biotin_lipoyl LIG_SUMO_SBM_1 b , LIG_WW_Pin1_4, MOD_ProDKin_1, MOD_SUMO b Q8R6D6 Serine protease FN1950 Peptidase_S8 a , Autotransporter a Peptidase_S8 LIG_FHA_2, LIG_SH2_STAT5, LIG_SUMO_SBM_1 b , MOD_CK1_1, MOD_CK2_1 b , MOD_GSK3_1, MOD_PKA_2...”
- Quantitative Proteomic Analysis of Outer Membrane Vesicles from Fusobacterium nucleatum Cultivated in the Mimic Cancer Environment
Zhang, Microbiology spectrum 2023 - “...decarboxylase gamma chain (D5RC28), glutaconyl-CoA decarboxylase subunit alpha (D5RC23), and putative glutaconyl-CoA decarboxylase subunit gamma (D5RC27), were significantly downregulated. Only 4-hydroxybutyrate coenzyme A transferase (D5RAM8) was downregulated in the 4-aminobutyric acid pathway. Furthermore, the synthesis of crotonyl-CoA was inhibited in the above-mentioned three butyrate synthesis pathways....”
- Perturbed human sub-networks by Fusobacterium nucleatum candidate virulence proteins
Zanzoni, Microbiome 2017 - “..., MOD_PIKK_1, MOD_PKA_2 Q8R5P1 DNAse I homologous protein DHP2 FN0891 Exo_endo_phos Exo_endo_phos LIG_SH2_GRB2, TRG_ENDOCYTIC_2 b Q8R5Y8 Biotin carboxyl carrier protein of glutaconyl-COA decarboxylase FN0200 Biotin_lipoyl Biotin_lipoyl LIG_SUMO_SBM_1 b , LIG_WW_Pin1_4, MOD_ProDKin_1, MOD_SUMO b Q8R6D6 Serine protease FN1950 Peptidase_S8 a , Autotransporter a Peptidase_S8 LIG_FHA_2, LIG_SH2_STAT5, LIG_SUMO_SBM_1...”
CA54_58530 pyruvate carboxylase from Symmachiella macrocystis
38% identity, 6% coverage
TGME49_284190 pyruvate carboxylase from Toxoplasma gondii ME49
36% identity, 5% coverage
- Apical annuli are specialised sites of post-invasion secretion of dense granules in <i>Toxoplasma</i>
Chelaghma, eLife 2024 - “...derived from the abundances of two proteins, acetyl-CoA carboxylase ACC1 (TGME49_221320) and pyruvate carboxylase PC (TGME49_284190). Both proteins are highly expressed, endogenously biotinylated, and reside in the matrix of subcellular compartments, the apicoplast and mitochondrion for ACC1 and PC, respectively, where they are not accessible to...”
- Stable endocytic structures navigate the complex pellicle of apicomplexan parasites
Koreny, Nature communications 2023 - “...factors derived from the abundances of two proteins, acetyl-CoA carboxylase ACC1 (TGME49_221320) and pyruvate carboxylase (TGME49_284190). Both proteins are highly expressed, endogenously biotinylated, and reside in the matrix of subcellular compartments, the apicoplast and mitochondrion for ACC1 and PC, respectively, where they are not accessible to...”
- Stable and ancient endocytic structures navigate the complex pellicle of apicomplexan parasites
Koreny, 2022 - Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presence of a divergent cytochrome c oxidase
Seidi, eLife 2018 - “...of biotin was observed in Figure 1E, given that pyruvate carboxylase is present in mitos (TGME49_284190; with >350 spectra in the author's dataset at ToxoDB) and others observe staining in IFAs with streptavidin (e.g. Jelenska 2001). Please comment. Also, this protein is not part of Supplementary...”
AYM39_08700 sodium-extruding oxaloacetate decarboxylase subunit alpha from Methylomonas sp. DH-1
45% identity, 10% coverage
H0Y4B9 propionyl-CoA carboxylase (Fragment) from Homo sapiens
44% identity, 37% coverage
- Standardization of a protocol for shotgun proteomic analysis of saliva
Ventura, Journal of applied oral science : revista FOB 2018 - “...protein 4 138.40 8.74 P12273 Prolactin-inducible protein 31682.10 76.71 Q16378 Proline-rich protein 4 312.60 21.64 H0Y4B9 Propionyl-CoA carboxylase alpha chain_ mitochondrial (Fragment) 231.31 20.90 P07602 Prosaposin 205.84 9.35 D6RDZ2 Protein FAM193B (Fragment) 266.86 35.56 Q14320 Protein FAM50A 176.55 10.62 Q5VT40 Protein FAM78B 141.80 10.73 Q8N7I0 Protein...”
5ks8C / Q1H157 Crystal structure of two-subunit pyruvate carboxylase from methylobacillus flagellatus (see paper)
39% identity, 11% coverage
- Ligands: pyruvic acid; manganese (ii) ion (5ks8C)
EF3325 sodium ion-translocating decarboxylase, biotin carboxyl carrier protein from Enterococcus faecalis V583
40% identity, 47% coverage
Q1H157 pyruvate carboxylase (EC 6.4.1.1) from Methylobacillus flagellatus (see paper)
39% identity, 11% coverage
Pnuc_0913 acetyl-CoA carboxylase, biotin carboxylase from Polynucleobacter sp. QLW-P1DMWA-1
51% identity, 8% coverage
3bg5B / A0A0H3JRU9 Crystal structure of staphylococcus aureus pyruvate carboxylase (see paper)
36% identity, 6% coverage
- Ligands: 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal; manganese (ii) ion; pyruvic acid (3bg5B)
MAB_3643 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Mycobacteroides abscessus ATCC 19977
MAB_3643 Probable bifunctional protein acetyl-/propionyl-CoA carboxylase (alpha chain) AccA3 from Mycobacterium abscessus ATCC 19977
34% identity, 11% coverage
4hnvB / A0A0H3JRU9 Crystal structure of r54e mutant of s. Aureus pyruvate carboxylase (see paper)
36% identity, 6% coverage
- Ligands: 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal; manganese (ii) ion (4hnvB)
EY04_RS01835 dihydrolipoyllysine-residue acetyltransferase from Pseudomonas chlororaphis
43% identity, 9% coverage
Tfu_1228 putative acyl-CoA carboxylase complex A subunit from Thermobifida fusca YX
38% identity, 10% coverage
- The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
Winkelman, Frontiers in molecular biosciences 2022 - “...CT subunit Tfu_2555 AcCCase E AcCCase E subunit Tfu_2556 ACCase B Additional ACCase CT subunit Tfu_1228, Tfu_1215 BCCP Biotin Carboxyl-Carrier Protein Tfu_1513 LCCase LCCase Tfu_1530 AcpP Acyl-carrier protein (ACP) Tfu_1975 MCAT Malonyl-CoA:ACP transacylase Tfu_1231, Tfu_1973 FabH 3-ketoacyl-ACP synthase III Tfu_1229, Tfu_1974 FabF 3-ketoacyl-ACP synthase III isozyme...”
- “...genome identified additional genes that encode homologs of biotin-containing carboxylase proteins, namely Tfu_0947, Tfu_0948, Tfu_1215, Tfu_1228, Tfu_1513, and Tfu_1530. The adjacent Tfu_0947 and Tfu_0948 genes suggest that they are on a single operon, with Tfu_0947 encoding a subunit with the BC and BCCP domains, and Tfu_0948...”
- Proteomics-based metabolic modeling and characterization of the cellulolytic bacterium Thermobifida fusca
Vanee, BMC systems biology 2014 - “...whereas the proteomics version clearly shows its presence (EC 6.4.1.1, Gene ID: Tfu_2557, Tfu_1530, Tfu_0947, Tfu_1228). In addition, most of the amino acid pathways were fully or partially incomplete in Tfu_v2. Thus, the Tfu_v2 model had artificially high fluxes through transport reactions to uptake external nutrients...”
3bg5A / A0A0H3JRU9 Crystal structure of staphylococcus aureus pyruvate carboxylase (see paper)
36% identity, 6% coverage
- Ligands: manganese (ii) ion; pyruvic acid; adenosine-5'-triphosphate; 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal (3bg5A)
PG1609 methylmalonyl-CoA decarboxylase, gamma subunit from Porphyromonas gingivalis W83
47% identity, 44% coverage
- The <i>Bacteroidetes</i> Q-Rule: Pyroglutamate in Signal Peptidase I Substrates
Bochtler, Frontiers in microbiology 2018 - “...gingipains and the biotin containing 15 kDa biotin carboxyl carrier protein (AccB alias MmdC or PG1609) as OM and IM specific markers, respectively ( Nguyen et al., 2007 ; Lasica et al., 2016 ) (data not shown). Generation of P. gingivalis RgpB Deletion Mutant For generation...”
- “...the exclusive presence of the biotin-containing 15-kDa biotin carboxyl carrier protein (AccA alias MmdC or PG1609) and gingipains in the IM and OM, respectively ( Veith et al., 2014 ) (data not shown). QC activity of the fractions was then measured using the enzyme-coupled assay already...”
- Protein Analysis of Sapienic Acid-Treated Porphyromonas gingivalis Suggests Differential Regulation of Multiple Metabolic Pathways
Fischer, Journal of bacteriology 2016 - “...two components of which, methymalonyl coenzyme A (MmdA; PG1609) and the biotin carboxyl carrier protein (MmdC; PGN_0503), were upregulated in our studies. The...”
- Structural and functional probing of PorZ, an essential bacterial surface component of the type-IX secretion system of human oral-microbiomic Porphyromonas gingivalis
Lasica, Scientific reports 2016 - “...Fig. 2c ), and the biotin-containing 15-kDa biotin carboxyl carrier protein (AccB alias MmdC or PG1609) as an IM marker ( Fig. 2d ; see also ref. 32 ). The latter analysis revealed that the OM fractions obtained from the wild type and the mutant were...”
- Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
Moon, BMC microbiology 2014 - “...: Anaerobic/Fermentation PG0687 Succinate-semialdehyde dehydrogenase 1.76 PG0690 4-hydroxybutyrate CoA-transferase 1.66 PG0689 NAD-dependent 4-hydroxybutyrate dehydrogenase 1.58 PG1609 Methylmalonyl-CoA decarboxylase, gamma subunit 1.87 PG1612 Methylmalonyl-CoA decarboxylase, alpha subunit 1.71 PG1608 Methylmalonyl-CoA decarboxylase, beta subunit 1.64 PG0675 Indolepyruvate ferredoxin oxidoreductase, alpha subunit 1.53 PG1809 2-oxoglutarate oxidoreductase, gamma subunit 2.18...”
- Role of the Porphyromonas gingivalis extracytoplasmic function sigma factor, SigH
Yanamandra, Molecular oral microbiology 2012 - “...protein 0.852168 1.805212 6.034404 0.000085 12 PG1432 Sensor histidine kinase 0.839072 1.788899 8.275893 0.000009 12 PG1609 methylmalonyl-CoA decarboxylase, gamma s. 0.834169 1.782830 9.434488 0.000001 12 PG0536 Hypothetical protein 0.812835 1.756660 18.157552 0.000000 (1.5E-09) 12 PG0928 response regulator 0.812297 1.756005 15.231637 0.000000 (9.7E-09) 12 PG0520 chaperonin, 60...”
- Verification of a topology model of PorT as an integral outer-membrane protein in Porphyromonas gingivalis
Nguyen, Microbiology (Reading, England) 2009 - “...over streptavidin-agarose revealed the protein to be an oxaloacetate decarboxylase ( oadA gene; TIGR ID: PG1609). This protein has been predicted to be on the inner membrane and as biotin is an essential co-enzyme for carboxylases, the biotin-association with the oadA gene product on the inner...”
- “...IM fraction an oxaloacetate decarboxylase as determined by N-terminal sequencing ( oadA gene; TIGR ID: PG1609) detected by alkaline phosphatase (AP)-conjugated streptavidin ( Fig. 1B ) and the exclusive presence of lipopolysaccharide (LPS) in the OM fraction as detected by anti-LPS mAb 1B5 ( Fig. 1C...”
DUR1,2 / P32528 urea amidolyase (EC 6.3.4.6) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 6 papers)
DUR1_YEAST / P32528 Urea amidolyase; EC 6.3.4.6; EC 3.5.1.54 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
P32528 allophanate hydrolase (EC 3.5.1.54); urea carboxylase (EC 6.3.4.6) from Saccharomyces cerevisiae (see 3 papers)
YBR208C Dur1,2p from Saccharomyces cerevisiae
38% identity, 3% coverage
- function: Hydrolysis of urea to ammonia and CO(2)
catalytic activity: urea + hydrogencarbonate + ATP = urea-1-carboxylate + ADP + phosphate + H(+) (RHEA:20896)
catalytic activity: urea-1-carboxylate + H2O + 3 H(+) = 2 NH4(+) + 2 CO2 (RHEA:19029)
cofactor: biotin
subunit: Monomer - New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
Engel, Genetics 2022 - “...unnecessary punctuation ORF Old gene name New gene name YGL234W ADE5,7 ADE57 YER069W ARG5,6 ARG56 YBR208C DUR1,2 DUR12 YIL154C IMP2 IMP21 Although nonstandard historical names are maintained in SGD, any new names for yeast genes must conform to the standard format. The SGD Gene Registry (...”
- Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica
Dahlin, Frontiers in genetics 2019 - “...well as the upregulation of enzymes involved in nitrogen metabolism such as DUR1,2 (urea amidolyase, YBR208C) and DAL1 (allantoinase, YIR027C). Table 3 GO term enrichment in differentially expressed genes. Go terms enriched more than 2.5-fold. Indented GO terms in brackets are a sub-group of the preceeding...”
- Developing a set of strong intronic promoters for robust metabolic engineering in oleaginous Rhodotorula (Rhodosporidium) yeast species
Liu, Microbial cell factories 2016 - “...b 105 b 14 639 YBR041W DUR1 4446 25 303 b 109 b 12 1239 YBR208C LDP1 1256 10 115 bc 230 b 7 261 RHTO-05627 a Genbank numbers used for BLAST search and gene annotation b Predicted by transcriptomic results c Containing the first intron...”
- Comparative transcriptomic analysis reveals similarities and dissimilarities in Saccharomyces cerevisiae wine strains response to nitrogen availability
Barbosa, PloS one 2015 - “...responsible for the major part of volatile acetate ester production during fermentation "DUR1 , 2" YBR208C 11.1 Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in...”
- An alternative, arginase-independent pathway for arginine metabolism in Kluyveromyces lactis involves guanidinobutyrase as a key enzyme
Romagnoli, Molecular microbiology 2014 - “...putative amidase 14.7 KLLA0F04235g Conserved hypothetical protein/putative arginase 13.5 KLLA0E08119g Highly similar to S. cerevisiae YBR208C DUR1 2 urea amidolyase 6.1 KLLA0F14366g Similar to S. cerevisiae ARG3 ornithine carbamoyltransferase 4.1 KLLA0F00594g Highly similar to S. cerevisiae GDH3 NADP(+)-dependent glutamate dehydrogenase 3.9 KLLA0B05247g Highly similar to S....”
- Full dynamic range proteome analysis of S. cerevisiae by targeted proteomics
Picotti, Cell 2009 - “...YKL150W, YEL031W, YGL202W, YDL017W, YGR080W, YPL049C, YGL248W, YEL011W YMR170C, YCL017C 5121024 11 10 YHR107C, YGL100W, YBR208C, YPR118W, YJL172W, YBR283C, YCR088W, YGR256W, YJL026W, YCL030C YOL116W 256512 12 10 YCL017C, YOL116W, YNL161W, YJR051W, YKL068W, YHR138C, YGR232W, YMR199W, YOR267C, YJR134C YGL248W 128256 13 10 YKL141W, YHR074W, YLR330W, YDR436W, YKL129C,...”
- Prediction of protein-protein interactions using protein signature profiling
Mahdavi, Genomics, proteomics & bioinformatics 2007 - “...(= 22421438) proteins may point out a direction for further experimental validation. For example, proteins YBR208C and YGL062W are found interacting using our approach but they are not reported in the experimental dataset. Note that YBR208C contains seven domains, six of which are shared by YGL062W....”
- Comprehensive analysis of co-occurring domain sets in yeast proteins
Cohen-Gihon, BMC genomics 2007 - “...carboxylase, pyruvate carboxylase 1 and 2, urea amidolyase and HFA1 (SwissProt IDs YNR016C, YGL062W, YBR218C, YBR208C and YMR207C). The proteins share four domains: Carbamoyl-phosphate synthase L chain, Carbamoyl-phosphate synthetase large chain N-terminal, Biotin carboxylase C-terminal and Biotin/lipoyl attachment (Interpro IDs IPR005479, IPR005481, IPR005482 and IPR000089). (B)...”
- More
- Altered proteome in translation initiation fidelity defective eIF5G31R mutant causes oxidative stress and DNA damage
Ram, Scientific reports 2022 - “...P40043 RGI1 1.6674 0.0044 13 Q01454 CTF4 1.7217 0.0309 14 P46992 TOH1 1.7233 0.0489 15 P32528 DUR1,2 1.7275 0.0011 16 Q02725 VTC3 1.7513 0.0068 17 P47075 VTC4 1.8045 0.0040 18 P40159 YNL208W 1.8591 0.0191 19 Q04432 HSP31 1.8778 0.0026 20 P22943 HSP12 1.8912 0.0252 21 P43585...”
- Solving the Conundrum: Widespread Proteins Annotated for Urea Metabolism in Bacteria Are Carboxyguanidine Deiminases Mediating Nitrogen Assimilation from Guanidine
Schneider, Biochemistry 2020 - “...(UAAP1): UniProtKB Q87XD7, NCBI WP_005764729.1 Ps CgdB (UAAP2): UniProtKB Q87XD6, NCBI WP_005764727.1 Sc UAL: UniProtKB P32528, NCBI NP_009767.1 Ca UAL: UniProtKB A0A1D8PDC6, NCBI AOW26140.1 ABBREVIATIONS AH allophanate hydrolase CdgAB carboxyguanidine deiminase UAAP urea amidolyase associated proteins UAL urea amidolyase UC urea carboxylase REFERENCES [1] Wyatt BN...”
- Molecular evolution of urea amidolyase and urea carboxylase in fungi
Strope, BMC evolutionary biology 2011 - “...using blastp (version 2.2.17 [ 28 ]). For urea amidolyase search, the S. cerevisiae sequence (P32528) was used as a query. Search was performed using both the full sequence as well as only the amidase domain of this sequence. To search for urea carboxylase sequences, A....”
PGN_0503 putative biotin carboxyl carrier protein from Porphyromonas gingivalis ATCC 33277
47% identity, 43% coverage
DUR1.2 / AAC41643.1 urea amidolyase from Saccharomyces cerevisiae (see paper)
38% identity, 3% coverage
Pan258_31100 pyruvate carboxylase from Symmachiella dynata
38% identity, 6% coverage
Mal52_32150 pyruvate carboxylase from Symmachiella dynata
38% identity, 6% coverage
LA_2735 acetyl-CoA carboxylase biotin carboxyl carrier protein subunit from Leptospira interrogans serovar lai str. 56601
41% identity, 38% coverage
Pf6N2E2_2194 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) from Pseudomonas fluorescens FW300-N2E2
37% identity, 9% coverage
- mutant phenotype: Specifically important for utilizing L-Leucine.
SXYL_01821 pyruvate carboxylase from Staphylococcus xylosus
36% identity, 6% coverage
CA_C2660 pyruvate carboxylase from Clostridium acetobutylicum ATCC 824
CAC2660 Pyruvate carboxylase, PYKA from Clostridium acetobutylicum ATCC 824
38% identity, 6% coverage
- A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum
Yoo, mBio 2015 - “...glycolytic flux. Two fluxes involved in anaplerotic reactions, namely, those for pyruvate carboxylase (encoded by CA_C2660) and NADH-dependent malic enzymes (encoded by CA_C1589 and CA_C1596), could not be solved using our GSM analysis coupled with transcriptomic and proteomic analyses. All of the genes studied were transcribed...”
- Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum
Amador-Noguez, Applied and environmental microbiology 2011 - “...the expression of the pyruvate carboxylase gene (cac2660) actually increases during solventogenesis, despite the greatly decreased pyruvate carboxylase flux....”
- Transcriptional analysis of spo0A overexpression in Clostridium acetobutylicum and its effect on the cell's response to butanol stress
Alsaker, Journal of bacteriology 2004 - “...0.17 and/or 2 h (Fig. 7B) included pykA (CAC2660, encoding pyruvate carboxylase), an ABC transporter (CAC3101), a thioredoxin reductase gene (CAC3082), a...”
- Transcriptional analysis of butanol stress and tolerance in Clostridium acetobutylicum
Tomas, Journal of bacteriology 2004 - “...B. subtilis. In addition, a gene for pyruvate carboxylase (CAC2660 [Fig. 3B]) and two genes encoding a malic enzyme (CAC1589 and CAC1596) had gene expression...”
Q6CP22 allophanate hydrolase (EC 3.5.1.54); urea carboxylase (EC 6.3.4.6) from Kluyveromyces lactis (see 3 papers)
XP_454317 bifunctional urea carboxylase/allophanate hydrolase from Kluyveromyces lactis
38% identity, 3% coverage
H16_A3290 biotin carboxylase from Ralstonia eutropha H16
H16_A3290 acetyl-CoA carboxylase family protein from Cupriavidus necator H16
36% identity, 6% coverage
DET0119 oxaloacetate decarboxylase, alpha subunit from Dehalococcoides ethenogenes 195
42% identity, 10% coverage
CE0713 putative urea carboxylase from Corynebacterium efficiens YS-314
31% identity, 6% coverage
FHQ18_00520 pyruvate carboxylase from Deferribacter autotrophicus
39% identity, 6% coverage
GSU2428 pyruvate carboxylase from Geobacter sulfurreducens PCA
42% identity, 6% coverage
- Comparative proteomics of Geobacter sulfurreducens PCAT in response to acetate, formate and/or hydrogen as electron donor
Mollaei, Environmental microbiology 2021 - “...the route of converting malate to oxaloacetate via pyruvate (anaplerosis), we detected pyruvate carboxylase (Pyc; GSU2428) and the NADdependent malic enzyme (MaeB; GSU1700; Fig. 3 ; Fig. 2 , purple arrows). This pathway could potentially be an alternative for the malate dehydrogenase (Mdh), but at the...”
- “...phosphate dikinase (PpdK; GSU0580), PEP synthase (PpsA; GSU0803) and anaplerotic reactions by pyruvate carboxylase (Pyc; GSU2428) and phosphoenolpyruvate carboxykinase, GTPdependent (PckA; GSU3385). Variation in electron acceptors (Fe(III) or fumarate) has been reported to influence the contribution of different pathways in PEP synthesis (Yang et al .,...”
O67484 Pyruvate carboxylase c-terminal domain from Aquifex aeolicus (strain VF5)
aq_1520 pyruvate carboxylase c-terminal domain from Aquifex aeolicus VF5
39% identity, 10% coverage
PP0338, PP_0338 pyruvate dehydrogenase, dihydrolipoamide acetyltransferase component from Pseudomonas putida KT2440
45% identity, 11% coverage
- UEG Week 2024 Poster Presentations
, United European gastroenterology journal 2024 - UEG Week 2023 Poster Presentations
, United European gastroenterology journal 2023 - Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection
Wirth, Microbial biotechnology 2020 - “...a 5GCGCG3 overhang to the 5end of the first oligonucleotide. For example, if aceF ( PP_0338 ) is to be targeted, the sequences would be oligonucleotide 1 ( aceEF _F), 5 GCGCG CTC ATT CGC GTA CCT GAC AT C 3; and oligonucleotide 2 ( aceEF...”
- “...1979 ). In P.putida KT2440, the genes encoding AceE ( PP_0339 ) and AceF ( PP_0338 ) form an operon (Belda etal ., 2016 ). Considering its central role in sugar catabolism, genes encoding PDHc are considered a difficult knockout to achieve if relying on an...”
- Large-scale kinetic metabolic models of Pseudomonas putida KT2440 for consistent design of metabolic engineering strategies
Tokic, Biotechnology for biofuels 2020 - “...because this model was missing some of the alternative pathways from GEM. For example, aceF PP_0338 (encoding for acetyltransferase component of pyruvate dehydrogenase complex) and aceE PP_0339 (encoding for pyruvate dehydrogenase, E1 component) are essential in D2 because they encode for enzymes necessary for synthesizing acetyl-CoA...”
- Regulatory tasks of the phosphoenolpyruvate-phosphotransferase system of Pseudomonas putida in central carbon metabolism
Chavarría, mBio 2012 - “...hydratase (PP_1612); PykA, pyruvate kinase (PP_1362); AceE, pyruvate dehydrogenase subunit E1 (PP_0339); AceF, dihydrolipoamide acetyltransferase (PP_0338); LpdG, dihydrolipoamide dehydrogenase (PP_4187); GltA, citrate synthase (PP_4194); AcnA, aconitate hydratase 1 (PP_2112); AcnB, aconitate hydratase 2 (PP_2339); Icd, isocitrate dehydrogenase (PP_4011); Kgd, alpha-ketoglutarate decarboxylase (PP_4189); KgdB, dihydrolipoamide acetyltransferase (PP_4188);...”
MA0674 pyruvate carboxylase subunit B from Methanosarcina acetivorans C2A
41% identity, 12% coverage
- S-layer, surface-accessible, and concanavalin A binding proteins of Methanosarcina acetivorans and Methanosarcina mazei
Francoleon, Journal of proteome research 2009 - “...kDa cytoplasmic pyruvate carboxylase subunit B (oxaloacetate decarboxylase) in both Methanosarcina species ( i.e. , MA0674 and MM1827). This endogenously biotinylated subunit is homologous to the soluble biotin-containing subunit PYCB of Methanosarcina barkeri that is part of the 4 4 -type acetyl CoA-independent pyruvate carboxylase (PYC)...”
- “...6 MA0840 dipeptide ABC transporter, solute-binding protein 65 62.9 2 no * 1 Band 7 MA0674 pyruvate carboxylase, subunit B 75 63.2 4 no 0 Band 8 MA0674 pyruvate carboxylase, subunit B 182 63.2 11 no 0 MA0086 thermosome, subunit alpha 120 58.9 5 yes 0...”
4qshC Crystal structure of l. Monocytogenes pyruvate carboxylase in complex with cyclic-di-amp (see paper)
39% identity, 6% coverage
- Ligands: (2r,3r,3as,5r,7ar,9r,10r,10as,12r,14ar)-2,9-bis(6-amino-9h-purin-9-yl)octahydro-2h,7h-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide; manganese (ii) ion (4qshC)
NP_464597 pyruvate carboxylase from Listeria monocytogenes EGD-e
Q8Y846 Pyruvate carboxylase from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
lmo1072 highly similar to pyruvate carboxylase from Listeria monocytogenes EGD-e
LMRG_00534 pyruvate carboxylase from Listeria monocytogenes 10403S
39% identity, 6% coverage
- Pyruvate carboxylase plays a crucial role in carbon metabolism of extra- and intracellularly replicating Listeria monocytogenes.
Schär, Journal of bacteriology 2010 - GeneRIF: The pycA mutant is also unable to replicate in mammalian cells and exhibits high virulence attenuation in the mouse sepsis model.
- A decade of research on the second messenger c-di-AMP
Yin, FEMS microbiology reviews 2020 - “...0.5 M 8 0.2 M CAB11805 ABK70852 CAC99150 P37538 A0R352 Q8Y846 Choi et al. 2015 Campeotto et al. 2015; Muller, Hopfner and Witte 2015 Gundlach et al. 2015a...”
- SecA2 Associates with Translating Ribosomes and Contributes to the Secretion of Potent IFN-β Inducing RNAs
Teubner, International journal of molecular sciences 2022 - “...General function prediction only; 1.73 2.41 lmo1783 rplT 50S ribosomal protein L20 Translation 2.08 2.26 lmo1072 pycA Pyruvate carboxylase Energy production and conversion 2.08 2.20 lmo1356 Acetyl-CoA carboxylase biotin carboxyl carrier protein Lipid transport and metabolism 1.70 2.16 lmo1330 rpsO 30S ribosomal protein S15 Translation 2.32...”
- c-di-AMP modulates Listeria monocytogenes central metabolism to regulate growth, antibiotic resistance and osmoregulation
Whiteley, Molecular microbiology 2017 - “...; pstA , lmo2692 , LMRG_02239 ; cbpB , lmo1009 , LMRG_02109 ; pycA , lmo1072 , LMRG_00534 ; citZ , lmo1567 , LMRG_01400 ; citC , lmo1566 , LMRG_01401 ; citB , lmo1641 , LMRG_01325 . L. monocytogenes strain construction Strains were constructed by standard...”
- “...locus Protein name Uniprot Entry Locus amino acid change DP-L6544 1081790 T A pycA LMRG_00534 lmo1072 pyruvate carboxylase A0A0H3GJD4_LISM4 Val241Glu DP-L6545 1082155 G T pycA LMRG_00534 lmo1072 pyruvate carboxylase A0A0H3GJD4_LISM4 Val363Leu DP-L6546 1082155 G T pycA LMRG_00534 lmo1072 pyruvate carboxylase A0A0H3GJD4_LISM4 Val363Leu DP-L6547 1082168 G T...”
- Comparison of Surface Proteomes of Adherence Variants of Listeria Monocytogenes Using LC-MS/MS for Identification of Potential Surface Adhesins
Tiong, Pathogens (Basel, Switzerland) 2016 - “...M CY 1.22 (CY,E,C) CM No No 0 No No <1.8 0.39 5 16 3 lmo1072 * (2.1.2) Pyruvate carboxylase (128) M/S CY 2.5 (CM,E,C) CY No No 0 No No <1.8 0.26 4 22 1 lmo1325 * (3.7.3) Translation initiation factor IF-2 (85) CY CY...”
- “...lmo0724 Unknown 5.2 --- --- 13 lmo0898 * lmo0898 Unknown 5.2 --- >10 --- 14 lmo1072 * pycA Main glycolytic pathways 2.1.2 >10 Neut 15 lmo1272 lmo1272 Unknown 5.2 --- --- 16 lmo1325 * infB Initiation (translation) 3.7.3 >10 --- 17 lmo1360 * folD Metabolism of...”
- Transcriptional and phenotypic responses of Listeria monocytogenes to chlorine dioxide
Pleitner, Applied and environmental microbiology 2014 - “...lmo2427 lmo2428 lmo2506 lmo2507 lmo2569 Energy metabolism lmo1072 lmo2367 Fatty acid and phospholipid metabolism lmo0970 lmo1806 lmo1807 lmo2202 lmo2450 Protein...”
- Membrane chaperone SecDF plays a role in the secretion of Listeria monocytogenes major virulence factors
Burg-Golani, Journal of bacteriology 2013 - “...lmo0706 lmo0708 lmo0713 lmo0724 lmo0847 20bp upstream lmo1072 lmo1527 lmo1687 lmo1693 lmo1855 lmo1856 lmo2110 lmo2531 lmo2715 lmo2461 and lmo1606 PrfA main...”
- Pyruvate carboxylase plays a crucial role in carbon metabolism of extra- and intracellularly replicating Listeria monocytogenes
Schär, Journal of bacteriology 2010 - “...of noncoding RNA (data not shown). The pycA gene (lmo1072) is the first gene of a transcription unit that contains three additional genes (lmo1073 to lmo1075)...”
- “...to generate oxaloacetate (2), and only a pycA gene (lmo1072) encoding PYC, but no pckA gene for a PEP carboxykinase, was identified in the L. monocytogenes...”
- Listeria monocytogenes {sigma}B has a small core regulon and a conserved role in virulence but makes differential contributions to stress tolerance across a diverse collection of strains
Oliver, Applied and environmental microbiology 2010 - “...lmo0818 lmo0819 lmo0928 lmo0929 lmo0995 lmo1037 lmo1064 lmo1072 lmo1121 lmo1226 lmo1242 lmo1243 lmo1360 lmo1388 lmo1421 lmo1426 lmo1427 lmo1527 lmo1534 lmo1571...”
- Comparative transcriptome analysis of Listeria monocytogenes strains of the two major lineages reveals differences in virulence, cell wall, and stress response
Severino, Applied and environmental microbiology 2007 - “...lmo1096 lmo1482 lmo1600 lmo1600 lmo2524 lmo1952 lmo1072 lmo1917 lmo2363 lmo0482 GO term identification no. LISTERIA MONOCYTOGENES TRANSCRIPTOME COMPARISONS...”
- c-di-AMP modulates Listeria monocytogenes central metabolism to regulate growth, antibiotic resistance and osmoregulation
Whiteley, Molecular microbiology 2017 - “..., lmo2692 , LMRG_02239 ; cbpB , lmo1009 , LMRG_02109 ; pycA , lmo1072 , LMRG_00534 ; citZ , lmo1567 , LMRG_01400 ; citC , lmo1566 , LMRG_01401 ; citB , lmo1641 , LMRG_01325 . L. monocytogenes strain construction Strains were constructed by standard methods (...”
- “...EGD-e locus Protein name Uniprot Entry Locus amino acid change DP-L6544 1081790 T A pycA LMRG_00534 lmo1072 pyruvate carboxylase A0A0H3GJD4_LISM4 Val241Glu DP-L6545 1082155 G T pycA LMRG_00534 lmo1072 pyruvate carboxylase A0A0H3GJD4_LISM4 Val363Leu DP-L6546 1082155 G T pycA LMRG_00534 lmo1072 pyruvate carboxylase A0A0H3GJD4_LISM4 Val363Leu DP-L6547 1082168 G...”
Rru_A1941 acyl-CoA carboxylase alpha chain from Rhodospirillum rubrum ATCC 11170
41% identity, 9% coverage
MAP3404 AccA3 from Mycobacterium avium subsp. paratuberculosis str. k10
35% identity, 11% coverage
- Description of a novel adhesin of Mycobacterium avium subsp. paratuberculosis
Viale, BioMed research international 2014 - “...Rv3075c hpcH/hpaI Aldolase/ citrate lyase 921 Gu et al., 2003 (MAP) [ 36 ] 11 MAP3404 Rv3285 accA3 Carbamoyl-phosphate synthase subunit A 1,824 Mawuenyega et al., 2005 (Mtb) [ 35 ] 12 MAP3567 Rv0148 Hypothetical protein Short-chain dehydrogenases/reductases 864 He and de Buck, 2010 (MAP) [...”
- Iron-sparing response of Mycobacterium avium subsp. paratuberculosis is strain dependent
Janagama, BMC microbiology 2010 - “...ATP phosphoribosyltransferase 1.69 0.2 3.68 0.3 MAP2332c Fas (fatty acid synthase) 1.61 0.5 2.28 0.4 MAP3404 AccA3 (acetyl-/propionyl-coenzyme A) 1.45 0.1 2.18 0.2 MAP3698c succinate dehydrogenase 1.89 0.3 4.57 0.5 Cellular processes MAP1339 iron regulated conserved protein 1.62 0.2 0.78 0.3 MAP1653 thiol peroxidase 1.79 0.5...”
- The ability of Mycobacterium avium subsp. paratuberculosis to enter bovine epithelial cells is influenced by preexposure to a hyperosmolar environment and intracellular passage in bovine mammary epithelial cells
Patel, Infection and immunity 2006 - “...factors. For example, genes MAP0482, MAP1695c, MAP3404, MAP1259, and MAP2652c encode transcription-regulatory proteins. Among other differentially expressed...”
- “...MAP0462, which encodes a urease alpha subunit, and MAP3404, encoding a biotin carboxyl bifunctional carrier protein, are essential genes for survival based on...”
BBA_04309 pyruvate carboxylase from Beauveria bassiana ARSEF 2860
37% identity, 5% coverage
- Unveiling a Novel Role of Cdc42 in Pyruvate Metabolism Pathway to Mediate Insecticidal Activity of Beauveria bassiana
Guan, Journal of fungi (Basel, Switzerland) 2022 - “...increase in pyruvate accumulation in the cdc42 mutant. Moreover, several upregulated genes (BBA_00790, BBA_09252, BBA_01687, BBA_04309, and BBA_08608) were involved in the participation of pyruvate in tricarboxylic acid (TCA) cycle and/or valine, leucine, and isoleucine biosynthesis. Three other upregulated genes (BBA_ 08607, BBA_02336, and BBA_07304) were...”
- Analysis of whitefly transcriptional responses to Beauveria bassiana infection reveals new insights into insect-fungus interactions
Xia, PloS one 2013 - “...shock protein 30 (HSP30) (ID: BBA_02057), isocitrate lyase (ICL) (ID: BBA_01125), pyruvate carboxylase (PC) (ID: BBA_04309), phosphoenolpyruvate carboxykinase (PEPCK) (ID: BBA_02833) and MAPKKK Ste11 (STE11) (ID: BBA_02280), respectively. Error bars: SE of the mean. Modulation of Cell Wall Synthesis and Regulation Related Genes Functional analysis revealed...”
- “...that, as critical carbon metabolism enzymes, phosphoenolpyruvate carboxykinase (BBA_02833), isocitrate lyase (BBA_01125) and pyruvate carboxylase (BBA_04309) were expressed at the up-regulated levels of 12.1, 22.4 and 3.41 folds at 72 hpi compared to 48 hpi, respectively. These enzymes have been recognized as vital components of several...”
Q9HPP8 biotin carboxylase (EC 6.3.4.14) from Halobacterium salinarum (see paper)
40% identity, 11% coverage
A6ZMR9 acetyl-CoA carboxylase, mitochondrial (EC 6.4.1.2) from Saccharomyces cerevisiae (strain YJM789) (see 2 papers)
38% identity, 3% coverage
HFA1 / P32874 acetyl-coenzyme A carboxylase subunit (EC 6.4.1.2) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 2 papers)
HFA1_YEAST / P32874 Acetyl-CoA carboxylase, mitochondrial; ACC; EC 6.4.1.2; EC 6.3.4.14 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
38% identity, 3% coverage
- function: Catalyzes the rate-limiting reaction in the mitochondrial fatty acid synthesis (FAS) type II pathway. Responsible for the production of the mitochondrial malonyl-CoA, used for the biosynthesis of the cofactor lipoic acid. This protein carries three functions: biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase.
catalytic activity: hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+) (RHEA:11308)
catalytic activity: N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) (RHEA:13501)
cofactor: biotin - Multiple horizontal gene transfer events and domain fusions have created novel regulatory and metabolic networks in the oomycete genome
Morris, PloS one 2009 - “...Ps108913 Lipid biosynthesis ATP citrate lyase none P53396 none Ps108918 Acetyl CoA carboxylase Q38970 Q13085 P32874 Ps108989 mRNA synthesis RNA polymerase II P18616 P24928 P04050 Ps109060 histidine synthesis Glutamine amidotransferase glutamine cyclase Q9SZ30 none P33734 Ps109110 riboflavin metabolism GTP cyclohydrolase II Dihydropteroate synthase Q9SJY9 none Q99258...”
W5QHK3 Methylcrotonoyl-CoA carboxylase 1 from Ovis aries
42% identity, 9% coverage
D8I9T6 Biotin/lipoyl attachment domain-containing protein from Brachyspira pilosicoli (strain ATCC BAA-1826 / 95/1000)
40% identity, 10% coverage
MAP_3404 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Mycobacterium avium subsp. paratuberculosis K-10
35% identity, 11% coverage
- Characterization of Ethanol Extracted Cell Wall Components of Mycobacterium avium Subsp. paratuberculosis
Bannantine, Veterinary sciences 2019 - “...acid desaturase 6C9 Murine mAb IgG1 MAP_3060c Electron transfer protein, -subunit 9H3 Murine mAb IgG2b MAP_3404 Acetyl-CoA, biotin carboxylase subunit 11G4 Murine mAb IgG1 MAP_3840 DnaK chaperone; Heat shock protein 14G11 Murine mAb IgG1 MAP_3976 Lipoprotein anchoring transpeptidase 7A6 Murine mAb IgG2a MAP_3936 Molecular chaperone, GroEL2...”
- “...C-terminal domain 13E1 Murine mAb IgG1 MAP_2121c Major membrane protein (MMP) 7C8 Murine mAb IgG2a MAP_3404 Acetyl-CoA, biotin carboxylase subunit p9270 Human mAb IgG1 LAM 2 Cell wall lipopolysaccharide p9045 Human mAb IgG1 LAM 2 Cell wall lipopolysaccharide 3993 Rabbit polyclonal ab EtOH 3 extract 3995...”
- Membrane and Cytoplasmic Proteins of Mycobacterium avium subspecies paratuberculosis that Bind to Novel Monoclonal Antibodies
Bannantine, Microorganisms 2018 - “...11 mAbs. This approach revealed that mAbs 7C8, 9H3, 12E4, 3G5, and 11B8 all detect MAP_3404 encoding the biotin carboxylase subunit of acetyl-CoA carboxylase, while mAbs 7A6, 11F8, and 10C12 detect the GroEL2 chaperonin (MAP_3936), 6C9 detects electron transfer flavoprotein (MAP_3060c), and 14G11 detects MAP_3976, a...”
- “...the epitope location. Peptide libraries were constructed (Thermo Scientific) for 2 Map proteins, MAP_2698c, and MAP_3404. The peptide lengths, sequences, location within each array, and degree of amino acid overlap are indicated in supplemental Table S2 . The design strategies resulted in 86 total peptides for...”
nfa9890 putative acyl-CoA carboxylase alpha subunit from Nocardia farcinica IFM 10152
36% identity, 11% coverage
- Glycosylation and subsequent malonylation of isoflavonoids in E. coli: strain development, production and insights into future metabolic perspectives
Koirala, Journal of industrial microbiology & biotechnology 2014 (PubMed)- “...genes for the acyl-CoA carboxylase and subunits (nfa9890 and nfa9940), biotin ligase (nfa9950), and acetyl-CoA synthetase (nfa3550) from Nocardia farcinia were...”
- “...from Glycine max This study pACYC104 pACYCDuetl carrying nfa9890, T7-rbs-nfa9940, nfa9950, and T7-rbs-nfa3550 from N. fatcinic [17] 13 1650 J Ind Microbiol...”
- Production of 7-O-methyl aromadendrin, a medicinally valuable flavonoid, in Escherichia coli
Malla, Applied and environmental microbiology 2012 - “...genes for the acyl-CoA carboxylase and subunits (nfa9890 and nfa9940), biotin ligase (nfa9950), and acetyl-CoA synthetase (nfa3550) from Nocardia farcinica were...”
- “...and 4cl-2 from P. crispum pACYCDuet-1 carrying nfa9890 from N. farcinica pACYCDuet-1 carrying nfa9890 and nfa9950 from N. farcinica pACYCDuet-1 carrying...”
PSPTO_5006 pyruvate dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase from Pseudomonas syringae pv. tomato str. DC3000
45% identity, 11% coverage
- A computational model of Pseudomonas syringae metabolism unveils a role for branched-chain amino acids in Arabidopsis leaf colonization
Tubergen, PLoS computational biology 2023 - “...yielded one ODHC-E1 component encoded by PSPTO_2199, one ODHC-E2 component encoded by either PSPTO_2200 or PSPTO_5006, and one ODHC-E3 component encoded by PSPTO_2201 ( S1 Table ), suggesting that enzymes of the ODHC complex could be part of the leucine degradation pathway and serve function equivalent...”
- “...the BCKDH enzymes [ 35 , 36 , 53 ]. In Pst DC3000, PSPTO_3860, PSPTO_5005, PSPTO_5006, and PSPTO_2201 encode the PDC enzymes, while PSPTO_2199, PSPTO_2200, and PSPTO_2201 encode the ODHC enzymes. Importantly, enzymes in these two complexes show a degree of promiscuity in substrate utilization, suggesting...”
D0LGS3 Carbamoyl-phosphate synthase L chain ATP-binding protein from Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2)
45% identity, 3% coverage
jadJ / Q9XCV7 acetyl CoA carboxylase monomer (EC 6.4.1.2) from Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745) (see paper)
35% identity, 12% coverage
SCO6271 acyl-CoA carboxylase complex A subunit from Streptomyces coelicolor A3(2)
Q9EWV4 biotin carboxylase from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
SCO4921 acyl-CoA carboxylase complex A subunit from Streptomyces coelicolor A3(2)
32% identity, 12% coverage
- Genome Analysis of a Variant of Streptomyces coelicolor M145 with High Lipid Content and Poor Ability to Synthetize Antibiotics
Dulermo, Microorganisms 2023 - “...of the nine genes of S. coelicolor M145 encoding putative acyl/acetyl/propionyl CoA carboxylases, sco4921 and sco6271 , were deleted in the TD variant. These enzymes, that catalyze the first step of fatty acid biosynthesis, are usually constituted by two subunits: and . In S. coelicolor ,...”
- “...well as SCO4921 (AccA2, acyl-CoA carboxylase subunit ), SCO4926 (PccB, propionyl-CoA carboxylase subunit ) and SCO6271 (Acc1, acyl-CoA carboxylase subunit ). AcetylCoA carboxylase catalyzes the carboxylation of acetyl-CoA to produce malonyl-CoA yielding straight/iso fatty acids. Propionyl CoA carboxylase catalyzes the carboxylation of propionyl-CoA to methylmalonyl-CoA yielding...”
- Dissection of 3D chromosome organization in Streptomyces coelicolor A3(2) leads to biosynthetic gene cluster overexpression
Deng, Proceedings of the National Academy of Sciences of the United States of America 2023 - “...the one of 4.3 Mb. Interestingly, this region comprised three precursor supply genes (SCO6269 to SCO6271) for the synthesis of malonyl-CoA ( 51 ), the assembly unit for the polyketide synthase (PKS), and well-characterized regulation genes (SCO6265 to SCO6266) for -butyrolactone (GBL) signaling circuits, which hints...”
- Coelimycin Synthesis Activatory Proteins Are Key Regulators of Specialized Metabolism and Precursor Flux in Streptomyces coelicolor A3(2)
Bednarz, Frontiers in microbiology 2021 - “...CpkL (SCO6285) were the only members of the cpk cluster which were not detected. AccA1 (SCO6271) differs by only four amino acids from AccA2 (SCO4921), therefore it is not possible to distinguish accurately between these two proteins by the shotgun proteomics method. Almost all detected peptides...”
- “...biotin as a cofactor. In S. coelicolor A3(2) there are two almost identical subunits, AccA1 (SCO6271), encoded within cpk gene cluster, and essential AccA2 (SCO4921) ( Rodrguez et al., 2001 ). We have found that ACCase subunit(s) AccA2 and/or AccA1, subunits AccB (SCO5535) and CpkK (SCO6284),...”
- AccR, a TetR Family Transcriptional Repressor, Coordinates Short-Chain Acyl Coenzyme A Homeostasis in Streptomyces avermitilis
Lyu, Applied and environmental microbiology 2020 (secret) - Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor
Nitta, Frontiers in bioengineering and biotechnology 2020 - “...This observation also matches the high levels of expression of the two acetyl-CoA carboxylaseencoding genes, SCO6271 ( accA1 ) and SCO4921 ( accA2 ) in M1146 + ACT ( Figure 4B ). When malonyl-CoA levels were analyzed by LC-MS/MS, malonyl-CoA decreased with time, and the malonyl-CoA...”
- ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor
Li, Scientific reports 2015 - “...In addition to kasO and sco6268 promoters, it also bound at the intergenic regions of sco6271 - sco6272 , sco6275 - 6276 , and sco6282 - sco6283 , and the promoter of another regulatory gene sco6288 . Similarly, in the cda cluster, beside the CSR gene...”
- Crp is a global regulator of antibiotic production in streptomyces
Gao, mBio 2012 - “...pathway-specific activator; Red synthesis 590 G T CTCGCCCAC C A C (520) SCO6271-SCO6272 i 1.83 SCO6271 AccA1, acyl-CoA carboxylase complex; yCPK synthesis 51 G T G A GGAGAA T C TT (8) SCO6272 Scf, secreted FAD j -binding protein, yCPK synthesis 257 AA G A TTCTCC...”
- “...synthesis SCO5221-5222 SCO5222 Repressed EizA, putative lyase SCO5223 SCO5223 Repressed Putative cytochrome P450 SCO6271-6272 c SCO6271 Activated AccA1, acyl-CoA carboxylase complex; yCPK synthesis SCO6272 Activated Scf, secreted FAD d -binding protein; yCPK synthesis SCO6272 c SCO6272 Activated Scf, secreted FAD-binding protein; yCPK synthesis SCO6275-6276 c SCO6275...”
- Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture
Thomas, Molecular & cellular proteomics : MCP 2012 - “...led to increased expression of Act (42, 43). SCO6271 (AccA1), SCO4921 (AccA2), and SCO5535 (AccB) encode subunits of an acetyl-CoA carboxylase (44). SCO4921 and...”
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- High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties.
Menendez-Bravo, Biotechnology for biofuels 2016 - “...S. coelicolor A3 (2) SCO4921 Q9X4K7 pccB, propionyl-CoA carboxylase subunit S. coelicolor A3 (2) SCO4926 Q9EWV4 pccE , propionyl-CoA carboxylase subunit S. coelicolor A3 (2) SCO4925 Q9EWV8 sfp , phosphopantetheinyl transferase B. subtilis BSU03570 X63158 prpE , propionyl-CoA synthetase E. coli b0335 P77495 pMB20 was constructed...”
- σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription
Pospíšil, Communications biology 2024 - “...B (HrdB-dependent, Fig. 5 ), we tested SCO1595- pheS , SCO5393, SCO5535- accB , and SCO4921- accA2 as genes likely positively regulated by HrdB. Here, the selection was made based on the presence of predicted HrdB binding sequences upstream of the genes. Except for SCO5393, all...”
- “...be regulated by HrdB instead of E (SCO1595- pheS , SCO5393, SCO5535- accB , and SCO4921- accA2 ). The Figure description is the same as in Fig. 4 . The blue rectangles show the -10 region of HrdB-dependent promoters (if identified in ref. 4 ). The...”
- Genome Analysis of a Variant of Streptomyces coelicolor M145 with High Lipid Content and Poor Ability to Synthetize Antibiotics
Dulermo, Microorganisms 2023 - “...Biosynthesis Two of the nine genes of S. coelicolor M145 encoding putative acyl/acetyl/propionyl CoA carboxylases, sco4921 and sco6271 , were deleted in the TD variant. These enzymes, that catalyze the first step of fatty acid biosynthesis, are usually constituted by two subunits: and . In S....”
- “...catalyzes the carboxylation of propionyl-CoA to methylmalonyl-CoA yielding branched/ante-iso fatty acids ( Figure 5 ). SCO4921 and SCO6271 might constitute the and subunits of a carboxylase complex that would be absent in the TD variant. This leaves six genes encoding three functional carboxylase complexes, SCO2445/44, SCO2776/77...”
- Coelimycin Synthesis Activatory Proteins Are Key Regulators of Specialized Metabolism and Precursor Flux in Streptomyces coelicolor A3(2)
Bednarz, Frontiers in microbiology 2021 - “...cluster which were not detected. AccA1 (SCO6271) differs by only four amino acids from AccA2 (SCO4921), therefore it is not possible to distinguish accurately between these two proteins by the shotgun proteomics method. Almost all detected peptides can be attributed to both of them. AccA1 and/or...”
- “...are two almost identical subunits, AccA1 (SCO6271), encoded within cpk gene cluster, and essential AccA2 (SCO4921) ( Rodrguez et al., 2001 ). We have found that ACCase subunit(s) AccA2 and/or AccA1, subunits AccB (SCO5535) and CpkK (SCO6284), and a small accessory protein AccE (SCO5536), were more...”
- The Onset of Tacrolimus Biosynthesis in Streptomyces tsukubaensis Is Dependent on the Intracellular Redox Status
Pires, Antibiotics (Basel, Switzerland) 2020 - “...STSU_10154 SCO5374 ATP synthase subunit epsilon (AtpC) 0.42 STSU_11515 SCO5044 Fumarate hydratase (FumB) 0.38 STSU_12400 SCO4921 putative acyl-CoA carboxylase complex A subunit 0.09 STSU_12680 SCO4855 succinate dehydrogenase iron-sulfur subunit (SdhB) 0.50 1 STSU_30056 SCO1391 Phosphoenolpyruvate-protein phosphotransferase (EI component) 0.45 Amino acid metabolism STSU_14552 SCO3345 Dihydroxy-acid dehydratase...”
- Iron competition triggers antibiotic biosynthesis in Streptomyces coelicolor during coculture with Myxococcus xanthus
Lee, The ISME journal 2020 - “...the acetyl-CoA synthetase (SCO6195) converted acetate to acetyl-CoA; and (iii) acetyl-CoA carboxylases (SCO2776, SCO2777, and SCO4921) converted acetyl-CoA to malonyl-CoA (Fig. 1e ). Considering that the transcription levels of genes involved in other acetyl-CoA-associated pathways, such as glycolysis and the citrate cycle, showed no differences between...”
- Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor
Nitta, Frontiers in bioengineering and biotechnology 2020 - “...high levels of expression of the two acetyl-CoA carboxylaseencoding genes, SCO6271 ( accA1 ) and SCO4921 ( accA2 ) in M1146 + ACT ( Figure 4B ). When malonyl-CoA levels were analyzed by LC-MS/MS, malonyl-CoA decreased with time, and the malonyl-CoA level was lower in M1146...”
- De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor
Marín, PloS one 2018 - “.... coelicolor chromosomal genes birA (biotin ligase, SCO4927 gene), accA2 (alpha subunit of acetyl-CoA carboxylase, SCO4921 gene) and accBE (beta and epsilon subunits of acetyl-CoA carboxylase, SCO5535 and SCO5536 genes respectively) [ 49 51 ]. These plasmids were amplified by PCR from S . coelicolor chromosomal...”
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FRAAL1210 biotin carboxylase; biotin carboxyl carrier protein (bifunctional protein) from Frankia alni ACN14a
41% identity, 11% coverage
- The Proteogenome of Symbiotic Frankia alni in Alnus glutinosa Nodules
Pujic, Microorganisms 2022 - “...a methyl-crotonoyl-CoA carboxylase (FRAAL2343, FC = 0.15) and two propionyl-CoA carboxylases (FRAAL5672, FC = 0.05; FRAAL1210, FC = 0.68). The only overabundant one was a phosphoribosylaminoimidazole carboxylase (FRAAL6664, FC = 4.9) involved in the synthesis of purines. A COG analysis ( Figure 2 ) revealed that...”
LAMA_EMENI / P38095 Putative urea carboxylase; Lactam utilization protein lamA; Urea amidolyase; EC 6.3.4.6 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
lamA putative urea amidolyase; EC 6.3.4.6 from Emericella nidulans (see 2 papers)
37% identity, 5% coverage
- function: Involved in the utilization of lactams. Required for the conversion of exogenous 2-pyrrolidinone (gamma-butyrolactam) to endogenous gamma-amino-n-butyrate (GABA).
catalytic activity: urea + hydrogencarbonate + ATP = urea-1-carboxylate + ADP + phosphate + H(+) (RHEA:20896)
cofactor: biotin - CharProtDB Description: Putative urea amidolyase with a predicted role in nitrogen metabolism; required for the utilization of lactams such as 2-pyrrolidinone; Source:AspGD
- Molecular evolution of urea amidolyase and urea carboxylase in fungi.
Strope, BMC evolutionary biology 2011 - “...the amidase domain of this sequence. To search for urea carboxylase sequences, A. nidulans sequence (P38095) was used as a query. To search for other urea carboxylase domain containing proteins, the S. cerevisiae Acc1 (Q00955) and Pyc (P11154), A. nidulans MccA (Q6T5L7), and Aspergillus related Neosartorya...”
Ccar_18725 pyruvate carboxylase from Clostridium carboxidivorans P7
38% identity, 6% coverage
METBIDRAFT_78404 pyruvate carboxylase from Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993
41% identity, 5% coverage
NP_013934 acetyl-CoA carboxylase HFA1 from Saccharomyces cerevisiae S288C
YMR207C Hfa1p from Saccharomyces cerevisiae
38% identity, 3% coverage
- Expression and evolution of the non-canonically translated yeast mitochondrial acetyl-CoA carboxylase Hfa1p.
Suomi, PloS one 2014 - GeneRIF: expression and evolution of the non-canonically translated yeast mitochondrial acetyl-CoA carboxylase Hfa1p
- High-throughput biochemical fingerprinting of Saccharomyces cerevisiae by Fourier transform infrared spectroscopy
Kohler, PloS one 2015 - “...ERG5 32 YDR297W SUR2 73 1 YMR205C PFK2 33 1 , 2 YDR492W IZH1 74 YMR207C HFA1 34 1 , 2 YDR503C LPP1 75 1 YMR246W FAA4 35 YER044C ERG28 76 YMR272C SCS7 36 YER061C CEM1 77 1 , 2 YNL323W LEM3 37 1 , 2...”
- “...YLL012W YEH1 24 YML008C ERG6 31 a , b YMR205C PFK2 33 a , b YMR207C HFA1 34 a , b YMR272C SCS7 36 YNL323W LEM3 37 a , b YNL280C ERG24 38 a , b YOR100C CRC1 45 YBL011W SCT1 55 YDL142C CRD1 66 a...”
- The yeast magmas ortholog pam16 has an essential function in fermentative growth that involves sphingolipid metabolism
Short, PloS one 2012 - “...ydl142c +++ Mitochondrial Lipid Metabolism ETR1 * ybr026c ++ X Mitochondrial Lipid Metabolism HFA1 * ymr207c +++ Mitochondrial Lipid Metabolism LAT1 * ynl071w +++ Mitochondrial Lipid Metabolism LIP2 * ylr239c + X Mitochondrial Lipid Metabolism LIP5 * yor196c +++ X Mitochondrial Lipid Metabolism LPD1 * yfl018c...”
- Identification of molecular pathways affected by pterostilbene, a natural dimethylether analog of resveratrol
Pan, BMC medical genomics 2008 - “...PI Four Kinase YMR165C PAH1 2.2 1.15 10 -3 Mg 2+ -dependent phosphatidate (PA) phosphatase YMR207C HFA1 2.0 3.22 10 -4 Mitochondrial acetyl-coenzyme A carboxylase YNL169C PSD1 -2.1 1.93 10 -3 Phosphatidylserine decarboxylase YNL231C PDR16 6.1 3.54 10 -4 by the multiple drug resistance regulator Pdr1p...”
- Comprehensive analysis of co-occurring domain sets in yeast proteins
Cohen-Gihon, BMC genomics 2007 - “...carboxylase 1 and 2, urea amidolyase and HFA1 (SwissProt IDs YNR016C, YGL062W, YBR218C, YBR208C and YMR207C). The proteins share four domains: Carbamoyl-phosphate synthase L chain, Carbamoyl-phosphate synthetase large chain N-terminal, Biotin carboxylase C-terminal and Biotin/lipoyl attachment (Interpro IDs IPR005479, IPR005481, IPR005482 and IPR000089). (B) Distribution of...”
- Metabolic functions of duplicate genes in Saccharomyces cerevisiae
Kuepfer, Genome research 2005 - “...Growth phenotypeb Esssential reactionc YKL106W YLR027C YNR016C YMR207C YOL086C YMR303C YMR083W YGL256W YBR145W YDR226W YER170W YOR374W YER073W YPL061W YLR089C...”
- Evidence of a new role for the high-osmolarity glycerol mitogen-activated protein kinase pathway in yeast: regulating adaptation to citric acid stress
Lawrence, Molecular and cellular biology 2004 - “...Lipid, fatty acid, and isoprenoid metabolism YER019W YMR207c YMR202w YLR056w YGL012w 3312 LAWRENCE ET AL. MOL. CELL. BIOL. TABLE 1--Continued Functional...”
- Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii
Brachat, Genome biology 2003 - “...5' intron Intron 15 5' RACE verified YMR084W/YMR085W Fusion FS ++ ++ ++ ++ Duplication YMR207C 5' extension FS 59 ++ ++ Duplication YMR269W 5' extension FS 69 ++ ++ Resequenced YNL083W 3' extension FS 51 ++ ++ ++ ++ [ 47 ] YNL130C Other intron...”
Q4WP18 Pyruvate carboxylase from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)
AFUA_4G07710, Afu4g07710 pyruvate carboxylase, putative from Aspergillus fumigatus Af293
37% identity, 5% coverage
- The proteomic signature of Aspergillus fumigatus during early development
Cagas, Molecular & cellular proteomics : MCP 2011 - “...Q4WX86 Q4X1G1 131 kDa 63 kDa 10 kDa 42 kDa Q4WT41 Q4WP18 Q4WXX9 Q4WRF2 29 kDa 11 kDa 22 kDa 16 kDa Q4WJK8 Q4X0M1 Q4WSZ2 Q9Y8D9 26 kDa 48 kDa Q8TGG6 Q4W9L9...”
- “...AFUA_6G08050 AFUA_3G11070 AFUA_4G07710 AFUA_6G01940 Q4WXX9 Q4WP18 Q4WCP3 Q4WN06 AFUA_2G17530 AFUA_5G09240 AFUA_5G09580 AFUA_8G00630 AFUA_4G08240 Q9UVW1 Q9Y8D9...”
- At the metal-metabolite interface in Aspergillus fumigatus: towards untangling the intersecting roles of zinc and gliotoxin
Traynor, Microbiology (Reading, England) 2021 - “...-Amino acid oxidase LaoA Unique n/a 22 40.5 AFUA_7G06810 Pyruvate carboxylase 3.55745 0.006854 73 69.7 AFUA_4G07710 Glycerol kinase, putative 3.49014 0.001297 26 50.9 AFUA_6G08470 Probable beta-glucosidase A BglA 3.21015 0.000732 29 33.5 AFUA_1G05770 Major allergen Aspf2 3.20795 0.006288 24 58.3 AFUA_4G09580 Peptidyl-arginine deiminase domain protein 3.18142...”
- Biotinylated Surfome Profiling Identifies Potential Biomarkers for Diagnosis and Therapy of Aspergillus fumigatus Infection
Jia, mSphere 2020 - “...Most likely, these represented the three known biotin-dependent carboxylases in A. fumigatus , encoded by Afu4g07710, Afu2g08670, and Afu5g08910, each of which showed high abundance according to the observed peptide spectrum matches (PSM; mass spectral count of repeated peptide identifications that serve as an approximate measure...”
- “...1.08 K40 S ; K69 S RodB Conidial cell wall hydrophobin 183 0.90 K105 S Afu4g07710 Putative pyruvate carboxylase 1193 8.38 1.61 K571 M ; K684 M ; K1004 M ; K1157 G Afu6g13250 c 60S ribosomal protein L31e 123 3.55 1.98 K30 M ; K59...”
- Molecular Mechanisms of Conidial Germination in Aspergillus spp
Baltussen, Microbiology and molecular biology reviews : MMBR 2020 (secret) - Extensive proteomic remodeling is induced by eukaryotic translation elongation factor 1Bγ deletion in Aspergillus fumigatus
O'Keeffe, Protein science : a publication of the Protein Society 2013 - “...31 AFUA_5G02370 AFUA_3G11070 AFUA_6G10650 AFUA_5G11230 AFUA_1G09020 AFUA_3G08940 AFUA_4G07710 AFUA_6G14460 11.5 11.5 11.5 22.2 22.1 21.8 21.6 21.6 5.83 6.08...”
- Development stage-specific proteomic profiling uncovers small, lineage specific proteins most abundant in the Aspergillus Fumigatus conidial proteome
Suh, Proteome science 2012 - “...glucanase BtgE (AFUA_8G05610), superoxide dismutase SodA (AFUA_5G09240), pyridoxine biosynthesis protein PyroA (AFUA_5G08090), and pyruvate carboxylase (AFUA_4G07710). BtgE is a covalently bound cell wall protein with a predicted role in degradation of glucans. In contrast to 0h enriched proteins, there is a much higher degree of correlation...”
- The proteomic signature of Aspergillus fumigatus during early development
Cagas, Molecular & cellular proteomics : MCP 2011 - “...AFUA_6G06770 AFUA_5G13450 AFUA_3G08380 AFUA_4G13120 AFUA_4G07710 AFUA_5G09230 AFUA_1G11480 AFUA_3G11070 AFUA_6G06750 AFUA_3G14490 AFUA_5G02910 AFUA_6G01940...”
- “...Q4WDM0 AFUA_2G10030 Q4X1G7 AFUA_6G08050 AFUA_3G11070 AFUA_4G07710 AFUA_6G01940 Q4WXX9 Q4WP18 Q4WCP3 Q4WN06 AFUA_2G17530 AFUA_5G09240 AFUA_5G09580 AFUA_8G00630...”
- Targeted disruption of nonribosomal peptide synthetase pes3 augments the virulence of Aspergillus fumigatus
O'Hanlon, Infection and immunity 2011 - “...43 6 33 14 7 42 AFUA_1G13740 AFUA_4G07710 AFUA_1G13500 AFUA_1G13500 AFUA_3G03020 AFUA_6G04740 AFUA_1G13490 AFUA_4G12730 AFUA_1G10570 AFUA_6G02750 2.9 2.2 3.1...”
- Integrative analysis of the heat shock response in Aspergillus fumigatus
Albrecht, BMC genomics 2010 - “...1.18 0.90 -0.09 0.44 Yes AFUA_4G07360 (cobalamin-independent methionine synthase MetH/D) 1.53 0.66 -1.08 -0.08 Yes AFUA_4G07710 (pyruvate carboxylase) 2.29 1.84 0.78 1.25 Yes AFUA_4G10460 (homocitrate synthase) 1.56 1.04 0.31 0.88 Yes AFUA_4G13120 (glutamine synthetase) 0.67 1.48 1.72 1.84 No Ubiquitination and proteolysis AFUA_4G09030 (aminopeptidase) 1.58 1.31...”
- “...synthase TRPB) -0.85/0.85 (90) -0.83/0.93 (75) AFUA_4G07360 (cobalamin-independent methionine synthase MetH/D) -0.83/0.81 (75) -0.83/0.96 (90) AFUA_4G07710 (pyruvate carboxylase) -0.54/0.96 (90) -0.49/0.93 (90) AFUA_4G10460 (homocitrate synthase) -0.73/0.86 (75) -0.60/0.89 (90) Protein biosynthesis/Translation AFUA_1G02030 (eukaryotic translation initiation factor 3 subunit EifCb) -0.86/0.89 (75) -0.89/0.88 (75) AFUA_6G12660 (40S ribosomal...”
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B0Y5W4 Pyruvate carboxylase from Aspergillus fumigatus (strain CBS 144.89 / FGSC A1163 / CEA10)
37% identity, 5% coverage
PA14_66310 dihydrolipoamide acetyltransferase from Pseudomonas aeruginosa UCBPP-PA14
42% identity, 11% coverage
A4W92_RS13735 dihydrolipoyllysine-residue acetyltransferase from Pseudomonas aeruginosa
42% identity, 11% coverage
NP_824513, SAV_3337 acyl-CoA carboxylase, alpha subunit from Streptomyces avermitilis MA-4680
33% identity, 12% coverage
- SAV742, a Novel AraC-Family Regulator from Streptomyces avermitilis, Controls Avermectin Biosynthesis, Cell Growth and Development
Sun, Scientific reports 2016 - “...encoding a putative GntR-family transcriptional regulator) 43 ; in fatty acid biosynthesis and degradation: accA3 (sav_3337 , encoding the alpha subunit of acyl-CoA carboxylase), accD4 (sav_3331 , encoding the beta subunit of acyl-CoA carboxylase), fadD5 (sav_1258 , encoding a putative acyl-CoA synthetase/long chain fatty acid: CoA...”
- The role of acyl-coenzyme A carboxylase complex in lipstatin biosynthesis of Streptomyces toxytricini
Demirev, Applied microbiology and biotechnology 2010 - “...S. toxytricini NRRL 15443; ScoAccA3, S. coelicolor A3(2) (NP_629074); SavAccA3, AccA3 of S. avermitilis MA-4680 (NP_824513); SprJadJ, JadJ of S. pristinaespiralis ATCC 25486 (ZP_05009963); SclJadJ; JadJ of S. clavuligerus ATCC 27064 (ZP_05005117); MtbAccA3; AccA3 of M. tuberculosis H37Rv (NP_217802); MboAccA3; AccA3 of M. bovis AF 2122/97...”
AOL_s00054g909 hypothetical protein from Orbilia oligospora ATCC 24927
36% identity, 6% coverage
ODP2_PSEAE / Q59638 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; EC 2.3.1.12 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
PA5016 dihydrolipoamide acetyltransferase from Pseudomonas aeruginosa PAO1
NP_253703 dihydrolipoamide acetyltransferase from Pseudomonas aeruginosa PAO1
42% identity, 11% coverage
- function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)
catalytic activity: N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + acetyl-CoA = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CoA (RHEA:17017)
cofactor: (R)-lipoate (Binds 2 lipoyl cofactors covalently.)
subunit: Forms a 24-polypeptide structural core with octahedral symmetry
disruption phenotype: Disruption inactivates the pyruvate dehydrogenase complex and causes an increase in pyruvate concentration and acidity of the culture medium. - Distinct transcriptome and traits of freshly dispersed <i>Pseudomonas aeruginosa</i> cells
Kalia, mSphere 2024 - “...0.966 0.87 PA4899 Probable aldehyde dehydrogenase 0.422 0.113 1.51 PA5015 Pyruvate dehydrogenase 0.87 0.625 0.305 PA5016 Dihydrolipoamide acetyltransferase 0.644 0.346 0.117 PA5110 Fructose-1,6-bisphosphatase 0.526 0.269 110 PA5131 Phosphoglycerate mutase 0.629 0.15 0.18 PA5192 Phosphoenolpyruvate carboxykinase 0.52 0.284 1.59 TCA cycle PA4333 Probable fumarase 1.15 1.16 0.32...”
- Identification of complex III, NQR, and SDH as primary bioenergetic enzymes during the stationary phase of Pseudomonas aeruginosa cultured in urine-like conditions
Hu, Frontiers in microbiology 2024 - “...Krebs cycle and glyoxylate cycle PA5015 NP_253702 2,649 aceE Pyruvate dehydrogenase Pyruvate dehydrogenase subunit E1 PA5016 NP_253703 1,644 aceF Dihydrolipoamide acetyltransferase PA1587 NP_250278 1,437 lpd 2-oxoglutarate dehydrogenase complex dihydrolipoyl dehydrogenase PA1787 NP_250478 2,610 acnB Aconitate synthase Aconitate hydratase B PA2623 NP_251313 1,257 icd Isocitrate dehydrogenase Isocitrate...”
- Discovery of coordinately regulated pathways that provide innate protection against interbacterial antagonism
Ting, eLife 2022 - “...Fold change (VSV-G/Ctrl ) Arc3B-VSV-G sample PA5113 (Arc3B ) 5.86 > 200 PA4385 1.62 2.29 PA5016 1.45 0.82 PA0090 1.17 0.72 PA3729 1.12 1.30 PA4761 1.12 0.71 PA5114 (Arc3A ) 1.12 N.D . PA5239 1.06 1.10 PA3861 1.00 1.10 PA4269 1.00 1.10 PA1092 0.95 0.78 PA0141...”
- “...PA5114 (Arc3A ) 1.44 N.D . PA4751 1.30 12.87 PA5556 1.26 3.55 PA3729 1.10 1.28 PA5016 1.10 0.62 PA4429 0.99 N.D. PA4942 0.96 6.36 PA2493 0.92 2.60 PA3160 0.90 N.D. PA4246 0.87 0.79 PA0090 0.85 0.53 PA1552 0.83 N.D. PA0077 0.81 5.33 PA2494 0.81 ND PA2976...”
- Feeding behaviour of Caenorhabditis elegans is an indicator of Pseudomonas aeruginosa PAO1 virulence
Lewenza, PeerJ 2014 - “...rRNA methylase B 26_C3 Gene PA4983 PA4983 Two-component response regulator A, C 80_B7 Gene aceF PA5016 Dihydrolipoamide acetyltransferase B 47_B5 Gene typA PA5117 Regulatory GTPase A 18_H10 Gene dctD PA5166 Two-component response regulator A, C 12_B5 Gene gcvT1 PA5215 Glycine-cleavage system protein T1 B 68_G8 Gene...”
- A theoretical and experimental proteome map of Pseudomonas aeruginosa PAO1
Lecoutere, MicrobiologyOpen 2012 - “...151 PA4974 Outer membrane protein MU OM 53.4 52 5.80 5.40 0.721 0.518 152 * PA5016 aceF Dihydrolipoamide acetyltransferase C C 56.7 67 5.23 5.11 0.694 0.046 153 PA5040 pilQ Fimbrial biogenesis U OM 77.4 74 5.48 5.25 0.621 0.257 154 PA5046 Malic enzyme C CM...”
- Analysis of Pseudomonas aeruginosa cell envelope proteome by capture of surface-exposed proteins on activated magnetic nanoparticles
Vecchietti, PloS one 2012 - “...Aspartyl-tRNA synthetase AspS 2 C,2 - P,1 lon PA1803 Lon protease 2 C,2 aceF aceB PA5016 Dihydrolipoyllysine-residue acetyltransferase 1 C,3 - P,1 rpoD PA0576 RNA polymerase sigma factor RpoD 1 C,3 ccoO1 PA1553 Cytochrome c oxidase 1 C,3 ftsY PA0373 Signal recognition particle receptor FtsY 2...”
- Microcolony formation by the opportunistic pathogen Pseudomonas aeruginosa requires pyruvate and pyruvate fermentation
Petrova, Molecular microbiology 2012 - “...PA2623, icd 4.44 PA1984 (Ad-DH) 4.52 PA1789, uspL 7.24 PA0836 (AckA) 4.40 PA4328, uspM 9.05 PA5016, aceF 2.55 PA1553, ccoQ1 24.7 PA1554, ccoN1 8.5 PA1555, ccoP2 6.9 PA1556, ccoQ2 6.1 PA1557, ccoN2 11.1 Fold changes refer to mifR versus wild-type. a respective transcripts have previously been...”
- Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient
Rao, Frontiers in microbiology 2011 - “...1.01 0.9193 0.83 273 PA5253 Regulatory protein AlgP 1 0.86 0.7890 0.66 0.1443 0.76 47 PA5016 Dihydrolipoamide acetyltransferase AceF 8 0.75 0.0127 0.80 0.0273 0.77 2 PA4385 GroEL protein 49 0.75 0.0000 0.69 0.0000 0.72 181 PA2853 Outer-membrane lipoprotein OprI precursor 3 0.69 0.1906 0.69 0.0376...”
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- Identification of complex III, NQR, and SDH as primary bioenergetic enzymes during the stationary phase of Pseudomonas aeruginosa cultured in urine-like conditions
Hu, Frontiers in microbiology 2024 - “...cycle and glyoxylate cycle PA5015 NP_253702 2,649 aceE Pyruvate dehydrogenase Pyruvate dehydrogenase subunit E1 PA5016 NP_253703 1,644 aceF Dihydrolipoamide acetyltransferase PA1587 NP_250278 1,437 lpd 2-oxoglutarate dehydrogenase complex dihydrolipoyl dehydrogenase PA1787 NP_250478 2,610 acnB Aconitate synthase Aconitate hydratase B PA2623 NP_251313 1,257 icd Isocitrate dehydrogenase Isocitrate dehydrogenase...”
- Protein Interaction Networks of Catalytically Active and Catalytically Inactive PqsE in Pseudomonas aeruginosa
Taylor, mBio 2022 - “...thrB thrB 1.365692537 Q02FF4 ureA ureA 1.264836648 Q9I3F5 acnA acnA 1.002888279 Q02V73 glyS glyS 1.008682243 Q59638 aceF aceF 1.067938829 B7UZY2 cysD cysD 1.092340172 Q02H29 murC murC 1.224317298 P27726 gap gapA 1.354759487 Q51363 nadB nadB 1.354759487 Q9I6M4 davT PA14_03450 1.411195433 B7UYA0 ackA ackA 1.486004021 P30718 groL groL...”
- “...aceE aceA 1.392137097 P30718 groL groL 1.415037499 Q02H54 groS groS 1.60823228 Q9I3D2 sucB sucB 1.694321257 Q59638 aceF aceF 1.805912948 Q51481 nirQ nirQ 5.158429363 10.1128/mbio.01559-22.2 FIGS2 Specificity-filtered interactions with eGFP-PqsE(WT) (a) and eGFP-PqsE(D73A) (b) in either WT or rhlR P. aeruginosa . Shapes indicate whether the interaction...”
- Proteomic Analysis of Resistance of Gram-Negative Bacteria to Chlorhexidine and Impacts on Susceptibility to Colistin, Antimicrobial Peptides, and Ceragenins
Hashemi, Frontiers in microbiology 2019 - “...(AceE) Catalyzes conversion of pyruvate to acetyl-CoA and CO 2 . Glycolytic process 3014.55 21 Q59638 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (AceF) Catalyzes acetyl-CoA and enzyme N 6 -(dihydrolipoyl)lysine to CoA and enzyme N 6 -( S -acetyldihydrolipoyl)lysine. Glycolytic process 40.67 22 Q9HZA7 Acetyl-coenzyme...”
PYC1_YEAST / P11154 Pyruvate carboxylase 1; Pyruvic carboxylase 1; PCB 1; EC 6.4.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
P11154 pyruvate carboxylase (EC 6.4.1.1) from Saccharomyces cerevisiae (see 2 papers)
NP_011453 pyruvate carboxylase 1 from Saccharomyces cerevisiae S288C
YGL062W Pyc1p from Saccharomyces cerevisiae
40% identity, 6% coverage
- function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second
catalytic activity: hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+) (RHEA:20844)
cofactor: biotin
cofactor: Zn(2+)
subunit: Homotetramer - Harnessing the optimization of enzyme catalytic rates in engineering of metabolic phenotypes
Razaghi-Moghadam, PLoS computational biology 2024 - “...involved a 1.6-fold increase in the k cat value of pyruvate carboxylase 1 (Uniprot ID P11154), with the closest comparable value coming from Lachancea waltii . Additionally, for increasing homoserine production in the eciML1515 model, OKO + proposed an engineering strategy involving a 1.3-fold increase in...”
- Regulation of d-Aspartate Oxidase Gene Expression by Pyruvate Metabolism in the Yeast Cryptococcus humicola
Imanishi, Microorganisms 2021 - “...Uniprot: A0A1B7SK73), PpPyc1 ( P. pastoris , P78992), Pyc1p, and Pyc2p ( S. cerevisiae , P11154 and P32327, respectively). Identical residues and amino acid substitutions with low and high similarity are indicated by asterisks, dots, and double dots, respectively. The regions boxed in red, blue, and...”
- Molecular evolution of urea amidolyase and urea carboxylase in fungi
Strope, BMC evolutionary biology 2011 - “...search for other urea carboxylase domain containing proteins, the S. cerevisiae Acc1 (Q00955) and Pyc (P11154), A. nidulans MccA (Q6T5L7), and Aspergillus related Neosartorya fischeri PccA (A1DF70) were used as query sequences. The urease sequence from A. fumigatus (Q6A3P9) was used as a query sequence to...”
- Conserved Glu40 and Glu433 of the biotin carboxylase domain of yeast pyruvate carboxylase I isoenzyme are essential for the association of tetramers.
Jitrapakdee, The international journal of biochemistry & cell biology 2007 (PubMed)- GeneRIF: Glu40 and Glu433 play essential roles in subunit interactions.
- Lager Yeast Design Through Meiotic Segregation of a Saccharomyces cerevisiae × Saccharomyces eubayanus Hybrid
Krogerus, Frontiers in fungal biology 2021 - “...and one a conservative inframe insertion ( Table S3 ). The affected genes include PYC1 (YGL062W), encoding a pyruvate carboxylase. Of the remaining, 20 were intergenic and eight were silent mutations. Structural variations (SVs) in the S. cerevisiae A62 parent strain were estimated from the long...”
- Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae
Cao, Microbial cell factories 2021 - “...Hxk2 Hexokinase 2 1.53 YDL021W Gpm2 Phosphoglycerate mutase family 1.41 YJR009C Tdh2 Glyceraldehyde-3-phosphate dehydrogenase 1.69 YGL062W Pyc1 Pyruvate carboxylase 1 1.31 Pentose-phosphate shunt, oxidative branch YHR183W Gnd1 Phosphogluconate dehydrogenase 1.64 YHR163W Sol3 6-Phosphogluconolactonase 1.51 BY4741 cultivated with 5 mM vanillin for 15h or without vanillin for...”
- HybridMine: A Pipeline for Allele Inheritance and Gene Copy Number Prediction in Hybrid Genomes and Its Application to Industrial Yeasts
Timouma, Microorganisms 2020 - “...( Table 3 and Figure 8 ). In fact, S. cerevisiae pyruvate decarboxylase genes, PYC1 (YGL062w) and PYC2 (YBR218C) are paralogs, and similarly the S. eubayanus XP_018223247.1 and XP_018222170.1 are annotated in UniProtKB as pyruvate decarboxylase genes. HybridMine accurately predicted all four of them as homologs....”
- “...YBR290W XP_018223316.1 XP_018223316.1 XP_018223316.1 SPGP0J00870 YBR215W XP_018223244.1 YBR215W YBR215W SPGP0J00840 YBR218C XP_018223247.1 YBR218C YBR218C SPGP0R01550 YGL062W XP_018222170.1 YGL062W YGL062W SPGP0I00870 YBR218C XP_018223247.1 XP_018223247.1 XP_018223247.1 SPGP0E03270 YGL062W XP_018222170.1 XP_018222170.1 XP_018222170.1...”
- The switch from fermentation to respiration in Saccharomyces cerevisiae is regulated by the Ert1 transcriptional activator/repressor
Gasmi, Genetics 2014 - “...-- QCR6 ADR1 YPR036W-A YFR033C YDR216W PYC1 YGL062W HXT2 YMR011W Function Phosphoenolpyruvate carboxykinase, key enzyme in the gluconeogenesis pathway...”
- “...ADR1 YDR216W MET13 YGL125W TMA10 YLR327C CYC8 YBR112C PYC1 YGL062W NAF1 YNL124W - YHR080C HXT2 YMR011W MIP6 YHR015W SFP1 YLR403W IGD1 YFR017C FAT3 YKL187C SFC1...”
- Adjustment of trehalose metabolism in wine Saccharomyces cerevisiae strains to modify ethanol yields
Rossouw, Applied and environmental microbiology 2013 - “...PDC5 PDC6 PYC1 PYC2 PCK1 YLR044c YLR134w YGR087c YGL062w YBR218c YKR097w Y12655 Y14091 Y14717 Y14429 Y13358 Y16013 Fermentation Alcohol dehydrogenase I Alcohol...”
- Yeast metabolic and signaling genes are required for heat-shock survival and have little overlap with the heat-induced genes
Gibney, Proceedings of the National Academy of Sciences of the United States of America 2013 - “...KCS1 YBL064C YKR055W PRX1 RHO4 YGL229C YNL307C YER039C YGL062W YOR235W YPR154W YCL022C YGR022C YPL197C YKR018C YOR039W YJL162C YLL001W SAP4 MCK1 HVG1 PYC1 IRC13...”
- Metabolic analyses elucidate non-trivial gene targets for amplifying dihydroartemisinic acid production in yeast
Misra, Frontiers in microbiology 2013 - “...YGR193C, YNL071W (pyruvate dehydrogenase) 2. YKL029C (NADPH-malic enzyme ME2) 2. YNL241C (glucose-6-phosphate dehydrogenase) 3. YBR218C, YGL062W (pyruvate carboxylase) 3. YGR043C, YLR354C (transaldolase in PPP) 4. YBR117C, YPR074C (transketolase in PPP) Gene knockdown in strain Fy DHA necessary to emulate this ExPa 1. YBR145W, YGL256W, YOL086C (alcohol...”
- Comparative transcriptome profiling analyses during the lag phase uncover YAP1, PDR1, PDR3, RPN4, and HSF1 as key regulatory genes in genomic adaptation to the lignocellulose derived inhibitor HMF for Saccharomyces cerevisiae
Ma, BMC genomics 2010 - “...+5.9 YPL156C PRM4 Pheromone-regulated protein proposed to be involved in mating +3.4 +2.8 +3.5 +3.5 YGL062W PYC1 Pyruvate carboxylase isoform +2.3 +2.6 +3.1 +2.1 YOR007C SGT2 Glutamine-rich cytoplasmic protein of unknown function +2.8 +2.5 +2.9 +3.0 YOR052C YOR052C Nuclear protein of unknown function +4.1 +2.6 +1.8...”
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N1P377 Pyruvate carboxylase from Saccharomyces cerevisiae (strain CEN.PK113-7D)
40% identity, 6% coverage
RSP_2191 Propionyl-CoA carboxylase alpha subunit from Rhodobacter sphaeroides 2.4.1
37% identity, 9% coverage
pycA putative pyruvate carboxylase or glutathione synthase from Emericella nidulans (see paper)
35% identity, 6% coverage
- CharProtDB Description: Putative pyruvate carboxylase or glutathione synthase; Source:AspGD
PA1400 probable pyruvate carboxylase from Pseudomonas aeruginosa PAO1
38% identity, 6% coverage
- Scalable and Chromatography-Free Synthesis of Efflux Pump Inhibitor Phenylalanine Arginine β-Naphthylamide for Its Validation in Wild-Type Bacterial Strains
Russo, ChemMedChem 2023 - “...our laboratory, including another general laboratory strain K799 [ 49 ] and two clinical isolates, PA1400, isolated from a patient with septicemia, and PA1651, isolated from a patient with cystic fibrosis. To narrow the scope of our experimentation, we focused on L,L-PAN 1 and D,D-PAN 1d...”
- “...absence of EPI was 0.5 g/mL instead of 1 g/mL for the laboratory strains. In PA1400, 1d was again mildly more potent than the L,L-isomer 1 , as 40 g/mL of 1 only provided a 2-fold potentiation while the same concentration of 1d provided 8-fold potentiation....”
- The opportunistic pathogen Pseudomonas aeruginosa exploits bacterial biotin synthesis pathway to benefit its infectivity
Shi, PLoS pathogens 2023 - “.... aeruginosa . Genome-wide mining of P . aeruginosa reveals five putative biotinylated enzymes, namely PA1400, PA2012, PA2891, PA4847, and PA5435 ( S1 Table ). D. Streptavidin blot analyses for crude extracts of E . coli and P . aeruginosa . Unlike E . coli displaying...”
- “...with the presence of multiple biotinylated proteins. E. SDS-PAGE (12%) profile for three biotinylated enzymes (PA1400, PA2012, and PA2891). Three of five predictive biotinylated enzymes were overexpressed, purified, and judged with SDS-PAGE (12%). F. Western blot analysis of the three recombinant proteins (PA1400, PA2012, and PA2891)...”
- Diastereoselective synthesis of cyclic tetrapeptide pseudoxylallemycin A illuminates the impact of base during macrolactamization
Fumo, Organic & biomolecular chemistry 2023 - “...aeruginosa, Strain PAK, NR-51337 and Pseudomonas aeruginosa, Strain Pa1400, NR-51335. We thank the Hancock Laboratory at the University of British Columbia for...”
- Periodically Disturbing the Spatial Structure of Biofilms Can Affect the Production of an Essential Virulence Factor in Pseudomonas aeruginosa
Quinn, mSystems 2021 - “...obtained through BEI Resources, NIAID, NIH: Pseudomonas aeruginosa strains Shr42, NR-48982, EnvKY1, NR-51329, MX0560, NR-51334, Pa1400, NR-51335, Pa1651, NR-51336, PAK, NR-51337, Pa1414, and NR-51345. We thank Roberto Kolter for his kind gift of the pel strain, Natasha Kirienko for her kind gift of the pvd strain,...”
- Comparative Genomic Analyses Reveal Core-Genome-Wide Genes Under Positive Selection and Major Regulatory Hubs in Outlier Strains of Pseudomonas aeruginosa
Sood, Frontiers in microbiology 2019 - “...et al., 2017 ). The outlier strains showed five major hubs namely gltB (glutamate synthase), PA1400 (pyruvate carboxylase), polA (DNA polymerase I), gacS (histidine kinase) and guaA (GMP synthase) whereas the classical strains had gltB, dnaK (chaperone protein), guaB (inosine 5-monophosphate dehydrogenase), hemE (uroporphyrin decarboxylase) and...”
- “...Outliers gltB 239 Glutamate synthase Provides glutamate for the glutamine synthetase reaction, absent in animals PA1400 221 Pyruvate carboxylase Irreversible carboxylation of pyruvate to form oxaloacetate (OAA) polA 171 DNA polymerase I Prokaryotic DNA replication gacS 159 Histidine kinase Play a role in signal transduction across...”
- General and condition-specific essential functions of Pseudomonas aeruginosa
Lee, Proceedings of the National Academy of Sciences of the United States of America 2015 - “...oxidoreductase (mqoA, mqoB, PA1252), pyruvate carboxylase (PA1400, PA5435-PA5436), and isocitrate dehydrogenase (icd, idh). Lee et al. Concluding Remarks....”
- Structure and function of a single-chain, multi-domain long-chain acyl-CoA carboxylase
Tran, Nature 2015 - “...(strain PA14) as the model organism. The P. aeruginosa enzyme (locus name PA14_46320, homolog of PA1400 in P. aeruginosa PAO1) shares 59% amino acid sequence identity with R. palustris LCC. This organism also carries the multi-subunit ACC, MCC and geranyl-CoA carboxylase (GCC) 24 , 25 ,...”
- Microcolony formation by the opportunistic pathogen Pseudomonas aeruginosa requires pyruvate and pyruvate fermentation
Petrova, Molecular microbiology 2012 - “...Gene Fold change Gene Fold change PA0927, ldhA 6.73 PA1580, gltA 24.24 PA5170, arcD 6.68 PA1400 (PycA) 5.12 PA0795, prpC 13.64 PA5171, arcA 3.38 PA0854, fumC2 9.55 PA1562, acnA 39.69 PA5172, arcB 3.18 PA5046 (Mdh) 3.38 PA1787, acnB 10.05 PA5173, arcC 5.28 PA4333 (Fum) 8.33 PA2624,...”
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madF / O06930 malonate decarboxylase biotin-carrier protein subunit (EC 7.2.4.4) from Malonomonas rubra (see 2 papers)
TC 3.B.1.1.4 / O06930 MadF, component of Na+-transporting malonate decarboxylase from Malonomonas rubra (see paper)
38% identity, 89% coverage
PA14_46320 putative pyruvate carboxylase from Pseudomonas aeruginosa UCBPP-PA14
41% identity, 6% coverage
- Structure and function of a single-chain, multi-domain long-chain acyl-CoA carboxylase
Tran, Nature 2015 - “...using Pseudomonas aeruginosa (strain PA14) as the model organism. The P. aeruginosa enzyme (locus name PA14_46320, homolog of PA1400 in P. aeruginosa PAO1) shares 59% amino acid sequence identity with R. palustris LCC. This organism also carries the multi-subunit ACC, MCC and geranyl-CoA carboxylase (GCC) 24...”
- “...an LCC deletion mutant in P. aeruginosa A markerless deletion was generated for the gene PA14_46320 in P. aeruginosa PA14 using previously described methods 42 . Briefly, ~1 kb flanking regions for PA14_46320 were amplified using primers listed in Extended Data Table 3 and recombined into...”
MSMEG_0334 acetyl-/propionyl-coenzyme A carboxylase alpha chain from Mycobacterium smegmatis str. MC2 155
38% identity, 9% coverage
- Control of biotin biosynthesis in mycobacteria by a pyruvate carboxylase dependent metabolic signal
Lazar, Molecular microbiology 2017 - “...M. smegmatis identified the pyruvate carboxylases MSMEG_2412 and MSMEG_6648, as well as the acetyl/propionyl-CoA carboxylases MSMEG_0334, 1807, 5943, and 4716. Each of these acetyl/propionyl-CoA carboxylases are predicted to have a molecular weight between 60 and 75 kDa. The biotinylated proteins we detected with molecular weights of...”
- “...of another biotin-utilizing enzyme in trans , such as the acetyl/propionyl-CoA carboxylase alpha chain enzymes MSMEG_0334 or MSMEG_1807. Each of these proteins contains a BC and BCCP domain, but lacks a CT domain, which is present in the beta chain present on a separate polypeptide. In...”
3va7A / Q6CP22 Crystal structure of the kluyveromyces lactis urea carboxylase (see paper)
38% identity, 5% coverage
- Ligands: 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal; urea (3va7A)
E1BGC1 Methylcrotonyl-CoA carboxylase subunit 1 from Bos taurus
40% identity, 8% coverage
AAur_0187 urea carboxylase from Arthrobacter aurescens TC1
39% identity, 5% coverage
F1QYZ6 Pyruvate carboxylase from Danio rerio
36% identity, 6% coverage
- Association between Chromosome 4 and mercury accumulation in muscle of the three-spined stickleback (Gasterosteus aculeatus).
Calboli, Evolutionary applications 2021 - “...ENSGACG00000019674 F1Q6T6 groupVII_7337531 1.2610 5 4.90 Intron pcxb pyruvate carboxylase b Group VII: 7,239,8357,431,018 ENSGACG00000019710 F1QYZ6 groupX_1922387 2.2410 5 4.65 Down stream si:dkeyp92c9.2 Cyclindependent kinase 5 activator Group X: 1,920,9381,921,900 ENSGACG00000002355 Q1LWT0 groupX_11239053 1.9910 5 4.70 exon dlgap3 discs, large (Drosophila) homologassociated protein 3 Group X:...”
Gbem_0273 pyruvate carboxylase from Geobacter bemidjiensis Bem
41% identity, 6% coverage
Mal48_22860 pyruvate carboxylase from Thalassoglobus polymorphus
34% identity, 5% coverage
ML0726 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Mycobacterium leprae TN
P46392 Biotin-dependent acyl-coenzyme A carboxylase alpha3 subunit from Mycobacterium leprae (strain TN)
NP_301567 acetyl/propionyl CoA carboxylase [alpha] subunit from Mycobacterium leprae TN
36% identity, 11% coverage
- Mycobacterium leprae and host immune transcriptomic signatures for reactional states in leprosy
Das, Frontiers in microbiology 2023 - “...0.020 0.1918 0.2723 ML0611 0.000 0.080 0.230 0.1740 0.2657 ML0638 0.060 0.350 0.460 0.1855 0.0651 ML0726 0.000 0.030 0.280 0.0816 0.1436 ML0757 0.060 1.330 0.940 0.0006 0.1158 ML0840 0.030 1.030 1.160 0.0028 0.0004 ML0841 0.020 0.570 0.420 0.0792 0.3072 ML0885 0.040 0.360 0.560 0.0572 0.0869 ML0987...”
- Mycobacterium leprae Transcriptome During In Vivo Growth and Ex Vivo Stationary Phases
Ojo, Frontiers in cellular and infection microbiology 2021 - “...fas , ML1191) on the other hand was slightly upregulated, although fabG (ML2565) was not. ML0726 ( bccA ) involved in long chain fatty acid synthesis was also significantly upregulated under axenic conditions. It is also interesting to note that desA2 (ML1952) thought to be involved...”
- Fatty acid biosynthesis in actinomycetes
Gago, FEMS microbiology reviews 2011 - “...mycobacteria, although in M. leprae only the ortholog of accA3 in M. tuberculosis , namely Ml0726 , is found. This suggests that accA3 encodes the subunit of the essential ACCase complexes. The focus in M. tuberculosis has been on the three putative essential ACCases (ACCase 4,...”
- Diverse protein regulations on PHA formation in Ralstonia eutropha on short chain organic acids
Lee, International journal of biological sciences 2009 - “...synthase 4 37 (27) T37056 Streptomyces coelicolor Fatty acid biosynthesis Acetyl/propionyl-CoA carboxylase 3 30 (26) P46392 Mycobacterium Fatty acid biosynthesis Energy metabolism Enolase 2 1 19 (17) Q9F3P9 Streptococcus coelicolor Energy metabolism Transaldolase B 4 41 (23) P66955 Salmonella typhimurium Pentose pathway Amino acid biosynthesis Glutamine...”
- The role of acyl-coenzyme A carboxylase complex in lipstatin biosynthesis of Streptomyces toxytricini
Demirev, Applied microbiology and biotechnology 2010 - “...(NP_217802); MboAccA3; AccA3 of M. bovis AF 2122/97 (NP_856958); MleBccA; BccA of M. leprae TN (NP_301567); CdpAccBC; of C. diphtheriae NCTC 13129 (NP_939023); StxPccE; PccE of S. toxytricini NRRL 15443, ScoPccE; PccE of S. coelicolor A3(2) (NP_629078); SavPccE; PccE of S. avermitilis MA-4680 (NP_824508); SprPccE; PccE...”
PADG_04103 pyruvate carboxylase from Paracoccidioides brasiliensis Pb18
34% identity, 6% coverage
- Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection
Lacerda, Virulence 2017 - “...ADH alcohol dehydrogenase (PADG_04701), PDC pyruvate decarboxylase (PADG_00714), PEPCK PEP carboxykinase (PADG_08503), PC pyruvate carboxylase (PADG_04103), CS citrate synthase (PADG_08387), ACO aconitase (PADG_11845), SCD succinate dehydrogenase (PADG_08013), FUM fumarase (PADG_02592), MDH malate dehydrogenase (PADG_08054), ICL isocitrate lyase (PADG_01483), MLS malate synthase (PADG_04702), ECH enoyl-CoA hydratase (PADG_01209),...”
PAAG_00726 pyruvate carboxylase from Paracoccidioides lutzii Pb01
34% identity, 6% coverage
- Interaction of Isocitrate Lyase with Proteins Involved in the Energetic Metabolism in Paracoccidioides lutzii
Freitas, Journal of fungi (Basel, Switzerland) 2020 - “...aldolase 14,297.79 PAAG_02585 triosephosphate isomerase * 12,564.27 PAAG_02869 phosphoglycerate kinase 12,361.49 PAAG_06380 pyruvate kinase 8237.422 PAAG_00726 pyruvate carboxylase 5375.759 PAAG_08203 phosphoenolpyruvate carboxykinase 4991.925 Tricarboxylic-acid pathway PAAG_01534 pyruvate dehydrogenase E1 component subunit beta 3429.868 PAAG_07729 isocitrate dehydrogenase subunit 2 4581.662 PAAG_08449 malate dehydrogenase 3811.987 PAAG_00053 malate dehydrogenase...”
- “...18,095.4 PAAG_06380 pyruvate kinase 20,228.38 PAAG_02512 pyruvate decarboxylase * 18,851.39 PAAG_02050 pyruvate decarboxylase * 18,717.38 PAAG_00726 pyruvate carboxylase 13,367.75 PAAG_08203 phosphoenolpyruvate carboxykinase 23,108.45 PAAG_02682 fructose-1.6-bisphosphatase 14,274.43 PAAG_07986 phosphofructokinase 2 16,924.33 PAAG_07410 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 15,371.76 Tricarboxylic-acid pathway PAAG_00094 pyruvate dehydrogenase kinase 11,769.04 PAAG_01534 pyruvate dehydrogenase E1...”
HCBG_00107 pyruvate carboxylase from Histoplasma capsulatum G186AR
C0NAG1 Pyruvate carboxylase from Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432)
35% identity, 6% coverage
BAB1_0018 Biotin/lipoyl attachment:Biotin-requiring enzyme, attachment site:Carbamoyl-phosphate synthase L chain, ATP-binding:Carbamoyl... from Brucella melitensis biovar Abortus 2308
42% identity, 9% coverage
- ClpP protease modulates bacterial growth, stress response, and bacterial virulence in Brucella abortus
Sun, Veterinary research 2023 - “...UbiG, BAB1_0241, SucC, BAB1_1964, and Bpt, while the top 10 down-regulated proteins were ClpS, BAB1_0115, BAB1_0018, BAB1_0296, BAB1_0875, BAB1_1435, BAB1_1357, BAB2_0019, BAB1_1214, and BAB2_0547. Integrated analysis revealed the global roles of ClpP in B. abortus 2308 According to the proteomic and transcriptomic profiles, clpP deletion extensively...”
BMEI1925 acetyl-CoA carboxylase alpha chain / propionyl-CoA carboxylase alpha chain from Brucella melitensis 16M
42% identity, 9% coverage
Awo_c26160 pyruvate carboxylase from Acetobacterium woodii DSM 1030
39% identity, 5% coverage
pccA / Q3J4D9 propionyl-CoA carboxylase α subunit (EC 6.4.1.3) from Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (see paper)
37% identity, 9% coverage
Pden_3684 Carbamoyl-phosphate synthase L chain, ATP-binding from Paracoccus denitrificans PD1222
PDEN_RS18285 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Paracoccus denitrificans
35% identity, 9% coverage
DUR1,2 likely multifunctional urea amidolyase from Candida albicans (see paper)
35% identity, 4% coverage
- CharProtDB CGD description: Urea amidolyase, contains urea carboxylase and allophanate hydrolase activities needed to hydrolyze urea to CO2; required for utilization of urea as nitrogen source and for hyphal switch in macrophages; transcription is regulated by Nrg1p
A0A1D8PDC6 allophanate hydrolase (EC 3.5.1.54) from Candida albicans (see paper)
XP_713590 bifunctional urea carboxylase/allophanate hydrolase from Candida albicans SC5314
35% identity, 4% coverage
Tfu_2557 putative acyl-CoA carboxylase, alpha subunit from Thermobifida fusca YX
WP_011292980 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Thermobifida fusca YX
34% identity, 11% coverage
- The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
Winkelman, Frontiers in molecular biosciences 2022 - “...subunits Tfu_0947 ACCase B ACCase CT subunit Tfu_0948 AcCCase A AcCCase BC and BCCP subunit Tfu_2557 AcCCase B AcCCase CT subunit Tfu_2555 AcCCase E AcCCase E subunit Tfu_2556 ACCase B Additional ACCase CT subunit Tfu_1228, Tfu_1215 BCCP Biotin Carboxyl-Carrier Protein Tfu_1513 LCCase LCCase Tfu_1530 AcpP Acyl-carrier...”
- “...the T. fusca genome. Previous studies have experimentally characterized the T. fusca operon (Tfu_2555, Tfu_2556, Tfu_2557) that encodes the B, E, and A subunits of an acyl-CoA carboxylase (AcCCase) ( Shivaiah et al., 2021 ). This enzyme is promiscuous and can carboxylate acetyl-CoA, propionyl-CoA, and butyryl-CoA....”
- Kinetic, Structural, and Mutational Analysis of Acyl-CoA Carboxylase From Thermobifida fusca YX
Shivaiah, Frontiers in molecular biosciences 2020 - “...2017 ). Methods and Materials Recombinant Expression Vectors ORFs encoding the TfAcCCB (Tfu_2555; WP_011292978), TfAcCCA (Tfu_2557; WP_011292980), and TfAcCCE (Tfu_2556; WP_011292979) proteins were initially identified using The Thioester-active enzYme database (ThYme) ( http://www.enzyme.cbirc.iastate.edu/ ) (Cantu et al., 2010 , 2011 ; Chen et al., 2011 )....”
- “...). Based on these sequence similarities, the three T. fusca ORFs where characterized as: (a) Tfu_2557 (WP_011292980) encoding the TfAcCCA subunit (62.1 kDa) that appears to carry the BC and BCCP functional components; (b) Tfu_2555 (WP_011292978) encoding the TfAcCCB subunit (57.6 kDa) that carries the CT...”
- Proteomics-based metabolic modeling and characterization of the cellulolytic bacterium Thermobifida fusca
Vanee, BMC systems biology 2014 - “...autobuilt Tfu_v2 version whereas the proteomics version clearly shows its presence (EC 6.4.1.1, Gene ID: Tfu_2557, Tfu_1530, Tfu_0947, Tfu_1228). In addition, most of the amino acid pathways were fully or partially incomplete in Tfu_v2. Thus, the Tfu_v2 model had artificially high fluxes through transport reactions to...”
- Kinetic, Structural, and Mutational Analysis of Acyl-CoA Carboxylase From Thermobifida fusca YX
Shivaiah, Frontiers in molecular biosciences 2020 - “...). Methods and Materials Recombinant Expression Vectors ORFs encoding the TfAcCCB (Tfu_2555; WP_011292978), TfAcCCA (Tfu_2557; WP_011292980), and TfAcCCE (Tfu_2556; WP_011292979) proteins were initially identified using The Thioester-active enzYme database (ThYme) ( http://www.enzyme.cbirc.iastate.edu/ ) (Cantu et al., 2010 , 2011 ; Chen et al., 2011 ). The...”
- “...Based on these sequence similarities, the three T. fusca ORFs where characterized as: (a) Tfu_2557 (WP_011292980) encoding the TfAcCCA subunit (62.1 kDa) that appears to carry the BC and BCCP functional components; (b) Tfu_2555 (WP_011292978) encoding the TfAcCCB subunit (57.6 kDa) that carries the CT functional...”
atuF / Q9HZV6 cis-geranyl-CoA carboxylase α-subunit (EC 6.4.1.5) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
PA2891 probable biotin carboxylase/biotin carboxyl carrier protein from Pseudomonas aeruginosa PAO1
37% identity, 10% coverage
- The opportunistic pathogen Pseudomonas aeruginosa exploits bacterial biotin synthesis pathway to benefit its infectivity
Shi, PLoS pathogens 2023 - “.... Genome-wide mining of P . aeruginosa reveals five putative biotinylated enzymes, namely PA1400, PA2012, PA2891, PA4847, and PA5435 ( S1 Table ). D. Streptavidin blot analyses for crude extracts of E . coli and P . aeruginosa . Unlike E . coli displaying a well-known...”
- “...of multiple biotinylated proteins. E. SDS-PAGE (12%) profile for three biotinylated enzymes (PA1400, PA2012, and PA2891). Three of five predictive biotinylated enzymes were overexpressed, purified, and judged with SDS-PAGE (12%). F. Western blot analysis of the three recombinant proteins (PA1400, PA2012, and PA2891) using anti-6x His...”
- The Pseudomonas aeruginosa Isohexenyl Glutaconyl Coenzyme A Hydratase (AtuE) Is Upregulated in Citronellate-Grown Cells and Belongs to the Crotonase Family
Poudel, Applied and environmental microbiology 2015 - “...PA2014 PA2015 PA2886 PA2887 PA2888 PA2889 PA2890 PA2891 PA2892 PA4330 Gene Structure-Function of Pseudomonas aeruginosa AtuE corresponding to rat ECH catalytic...”
- Swarming of Pseudomonas aeruginosa is controlled by a broad spectrum of transcriptional regulators, including MetR
Yeung, Journal of bacteriology 2009 - “...revealed PA1843 (metH), PA2399 (pvdD), PA2413 (pvdH), PA2891, PA4144, and PA4223. Conversely, PA0633, PA0848, and PA5125 (ntrC) were negatively regulated by...”
- The atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine in Pseudomonas aeruginosa
Aguilar, Applied and environmental microbiology 2006 - “...PAO1::ISlacZ/hah (ID 10788), transposon insertion in ORF PA2891 PAO1::ISlacZ/hah (ID 8377), transposon insertion in ORF PA2893 PAO1::ISlacZ/hah (ID 11254),...”
- “...PAO1SM mutant obtained by Himar1::Gmr transposition in ORF PA2891, unable to grow on citronellol PAO1SM mutant obtained by Himar1::Gmr transposition in ORF...”
- Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa
Höschle, Microbiology (Reading, England) 2005 (PubMed)- “...at 37 uC. Insertion mutagenesis. Disruption of atuF (PA2891) in P. aerugi- nosa was carried out using pKnockout-G for rapid gene inactivation (Windgassen et...”
- “...gene products of liuD (PA2012; 74 kDa) and atuF (PA2891; 71 kDa), respectively (Figs 2 and 3). The three non-biotin-containing polypeptides (band nos 4/5 and 6,...”
- The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa
Díaz-Pérez, Applied and environmental microbiology 2004 - “...the GnyA protein (left). crotonoyl-CoA carboxylase, ORF PA2891) biotinylated subunits of their respective carboxylases. In agreement with data from the RT-PCR...”
Q7KN97 Pyruvate carboxylase from Drosophila melanogaster
33% identity, 5% coverage
- Pterostilbene Promotes Mean Lifespan in Both Male and Female Drosophila Melanogaster Modulating Different Proteins in the Two Sexes
Beghelli, Oxidative medicine and cellular longevity 2022 - “...dehydrogenase E1 component subunit beta 34 17 12 39.3 7.8 60 0.56 0.00012 MALES 649 Q7KN97 PCB Pyruvate carboxylase 61 92 65 130.8 6.81 312 1.5 0.001 650 Q7KN97 PCB Pyruvate carboxylase 62 116 70 130.8 6.81 385 1.67 0.005 788 H9XVM8 Dmel\CG1674 Uncharacterized protein, isoform...”
- Apocrine secretion in Drosophila salivary glands: subcellular origin, dynamics, and identification of secretory proteins
Farkaš, PloS one 2014 - “...14.7 co-chaperone endoplasmic reticulum CG15093 (Probable 3-hydroxyisobutyrate dehydrogenase) Q9V8M5 33.9 metabolism mitochondrial CG1516 (Pyruvate carboxylase) Q7KN97 130.8 metabolism lipid particle CG1523-PA Q9VAT2 69.6 scaffold cytoplasmic CG1640 (Pyridoxal phosphate-dependent aminotransferase) Q9VYD9 64.0 basal metabolism cytoplasmic CG16799 A1ZBX6 21.0 protein modification cytoplasmic CG17734 Q8INK7 10.3 signal transduction membrane(transmembral)...”
- Proteomic characterization of inbreeding-related cold sensitivity in Drosophila melanogaster
Vermeulen, PloS one 2013 - “...2D42D4 4303 CG1440, isoform A Q9W3F6 7E97E9 4311 RE37426p Q5U189 29F529F5 4703 CG1516, isoform E Q7KN97 46B446B4 5302 HMG coenzyme A synthase, isoform A Q7K4Q9 53C153C1 5401 CG5384 Q9VKZ8 31D431D5 5704 CG1516, isoform E See SN4703 See SN4703 5704 CG1516, isoform E See SN4703 See SN4703...”
pycB / Q6M0D1 pyruvate carboxylase subunit B (EC 6.4.1.1) from Methanococcus maripaludis (strain S2 / LL) (see paper)
MMP0340 pyruvate carboxylase subunit B from Methanococcus maripaludis S2
36% identity, 11% coverage
accC / A9W9X0 biotin carboxylase (EC 6.3.4.14) from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) (see paper)
A9W9X0 biotin carboxylase (EC 6.3.4.14) from Chloroflexus aurantiacus (see paper)
Caur_1378 acetyl-CoA carboxylase, biotin carboxylase from Chloroflexus aurantiacus J-10-fl
WP_012257262 acetyl-CoA carboxylase biotin carboxylase subunit from Chloroflexus aurantiacus
38% identity, 11% coverage
- Coassimilation of organic substrates via the autotrophic 3-hydroxypropionate bi-cycle in Chloroflexus aurantiacus
Zarzycki, Applied and environmental microbiology 2011 - “...genes encoding biotin carboxyl carrier proteins/protein domains (Caur_1378, 64.3 kDa; Caur_2832, 69.4 kDa; and Caur_3739, 19.3 kDa) of acetyl-CoA/propionyl-CoA...”
- “...The two upper bands correspond to fusion proteins Caur_1378 (64.3 kDa) and Caur_2832 (69.4 kDa), each comprising a biotin carboxyl carrier protein and a...”
- Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus
Tang, BMC genomics 2011 - “...3470) CO 2 -anaplerotic pathways tme (Caur_1614), pckA (Caur_2331), ppc (Caur_3161, Caur_3888) 3-hydroxypropionate cycle accC (Caur_1378, Caur_3421), accA (Caur_1647), accD (Caur_1648), accB (Caur_3739), mcr (Caur_2614), pcs (Caur_ 0613), pccB (Caur_2034, Caur_3435), mcee (Caur_3037), mut (Caur_1844, Caur_2508, Caur_2509), smtA (Caur_0179), smtB (Caur_0178), sdhBAC (Caur_1880 to Caur_1882), fh...”
- “...the 3HOP cycle were later characterized experimentally [ 4 ]: (1) acetyl-CoA carboxylase ( accC (Caur_1378 and Caur_3421, biotin carboxylase), accA (Caur_1647, -subunit), accD (Caur_1648, -subunit) and accB (Caur_3739) [ 79 ], (2) malonyl-CoA reductase ( mcr , Caur_2614) [ 80 ], (3) propionyl-CoA synthase (...”
- Chloroflexus aurantiacus acetyl-CoA carboxylase evolves fused biotin carboxylase and biotin carboxyl carrier protein to complete carboxylation activity
Shen, mBio 2024 - “...isoforms, and separate BCCP, CT, and CT subunits ( 4 ). The first BC isoform (WP_012257262, BC1) contains 596 amino acid residues, while the second BC isoform (WP_012259259, BC2) comprises 455 residues. During the transition from respiratory to phototrophic conditions, a simultaneous increase in the expression...”
- “...primary structures of C. aurantiacus BC1 and BC2. C. aurantiacus possesses two BC isoforms, BC1 (WP_012257262) and BC2 (WP_012259259). BC1 confers a fused BC (Met1-Glu452) and BCCP domain (Ala459-Lys596), which contains a hairpin (Thr473-Gly486) and a BCCP Core (Lys511-Gln587) at the C-terminus. Multiple sequence alignment revealed...”
Q0E9E2 Pyruvate carboxylase from Drosophila melanogaster
33% identity, 5% coverage
F9UTV9 Biotin carboxyl carrier protein of acetyl-CoA carboxylase from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
34% identity, 37% coverage
L7MAU7 Propionyl-CoA carboxylase alpha chain, mitochondrial (Fragment) from Rhipicephalus pulchellus
41% identity, 11% coverage
- Quantitative Proteomics Identifies Metabolic Pathways Affected by Babesia Infection and Blood Feeding in the Sialoproteome of the Vector Rhipicephalus bursa
Couto, Vaccines 2020 - “...potential protective antigens resulting in a vaccine candidate. The amino acid sequences of L7M1X7 and L7MAU7 were analyzed in silico in order to predict the protein localization (CELLO v.2.5 [ 50 ]), transmembrane domains (TMHMM v.2.0, based on a hidden Markov model [ 51 , 52...”
- “...a recombinant protein (based on L7M1X7, UB2N, 151 a.a.) and a peptide (VKTPEECVKIAQSIGYPVMIKASAGGGGKGMRIAWND based on L7MAU7, PCCA, 37 a.a.) were selected to be synthetically produced by GenScript Corporation (Piscataway, NJ, USA) for the polyclonal antibodies production and immunoassays. To increase peptide immunogenicity, an Imject Blue Carrier...”
bccp / P02904 methylmalonyl-CoA carboxyltransferase 1.3S biotinyl subunit (EC 2.1.3.1) from Propionibacterium freudenreichii subsp. shermanii (see 3 papers)
BCCP_PROFR / P02904 Methylmalonyl-CoA carboxyltransferase 1.3S subunit; Biotin carboxyl carrier protein of transcarboxylase; Transcarboxylase, 1.3S subunit; EC 2.1.3.1 from Propionibacterium freudenreichii subsp. shermanii (see paper)
PFREUD_18840, RM25_RS08835 biotin/lipoyl-containing protein from Propionibacterium freudenreichii subsp. shermanii CIRM-BIA1
41% identity, 54% coverage
- function: The biotinyl 1.3S subunit serves as a carboxyl carrier between the substrate-binding sites on the 12S and 5S subunits
catalytic activity: (S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate (RHEA:20764)
subunit: Transcarboxylase is composed of three subunits: 1.3S, 5S, and 12S. The core of the enzyme is composed of six 12S subunits. On each side of the core there are three pairs of 5S subunits. Each 5S dimer is attached to the core by two 1.3S subunits. Thus the total number of chains is 30 (6 + 12 + 12). - The Sec1-Munc18 protein VPS33B forms a uniquely bidirectional complex with VPS16B
Liu, The Journal of biological chemistry 2023 - “...of amino acids 49 to 123 of Propionibacterium shermanii transcarboxylase (methylmalonyl-CoA carboxyltransferase 1.3S subunit; UniProt: P02904), corresponding to its biotinyl-binding domain. The amino acid sequence of this biotinylation signal is AGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAM K METEINAPTDGKVEKVLVKERDAVQGGQGLIKIG When expressed in yeast, this sequence is recognized by theendogenous biotinylation machinery and...”
- Cysteine 893 is a target of regulatory thiol modifications of GluA1 AMPA receptors
von, PloS one 2017 - “...fragment encoding the entire 1.3.S subunit of Propionibacterium shermanii transcarboxylase (i.e., biotinylation target domain; UniProtKB P02904; residues 1123), obtained from the plasmid pEBB-PP [ 39 ] (a kind gift from Dr Kalle Saksela, University of Helsinki). SAP97 constructs represented the isoform i1b, i3. The expression construct...”
- The complete genome of Propionibacterium freudenreichii CIRM-BIA1, a hardy actinobacterium with food and probiotic applications
Falentin, PloS one 2010 - “...and PFREUD_18870, have the same length and share 76% and 99% similarity with Swissprot entries P02904 and Q70AC7 from P. freudenreichii subsp. shermanii W52, respectively. The 12S subunit, encoded by PFREUD_18860, shares 96% similarity with the N-terminal part (524 amino acids) of the Swiss-Prot entry Q8GBW6,...”
- Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity.
Sarkar, Proceedings of the National Academy of Sciences of the United States of America 2008 - Susceptibility and adaptive response to bile salts in Propionibacterium freudenreichii: physiological and proteomic analysis
Leverrier, Applied and environmental microbiology 2003 - “...P. freudenreichii Campylobacter jejuni P. freudenreichii CAA62838 NP_246193 1908232B CAA62838 NP_282143 P02904 3 6 6 5 4 8 49 42 65 77 44 37 29 31 19 28 42 54...”
- Structure and selectivity in post-translational modification: attaching the biotinyl-lysine and lipoyl-lysine swinging arms in multifunctional enzymes
Reche, The EMBO journal 1999 - “...carboxyl transferase (1.3S subunit of transcarboxylase) (AC P02904); Eco_E2p1, Eco_E2p2 and Eco_E2p3, outer (N-terminal), middle and innerlipoyl domains ,...”
- Aerobic adaptation and metabolic dynamics of <i>Propionibacterium freudenreichii</i> DSM 20271: insights from comparative transcriptomics and surfaceome analysis
Loivamaa, mSystems 2024 - “...(Table S1; Fig. 4 ). Propionate metabolism, TCA cycle, and respiratory complexes The gene cluster RM25_RS08835 to RM25_RS08850 encodes the key enzyme, a multimeric transcarboxylase (EC 2.1.3.1), methylmalonyl-CoA carboxytransferase (MMC), of the propionate-forming WoodWerkman cycle ( 53 ). Interestingly, this gene cluster was found to be...”
- The complete genome of Propionibacterium freudenreichii CIRM-BIA1, a hardy actinobacterium with food and probiotic applications
Falentin, PloS one 2010 - “...sequences [17] . In CIRM-BIA1 T , the 1.3S and the 5S subunits, encoded by PFREUD_18840 and PFREUD_18870, have the same length and share 76% and 99% similarity with Swissprot entries P02904 and Q70AC7 from P. freudenreichii subsp. shermanii W52, respectively. The 12S subunit, encoded by...”
CNAG_05907 pyruvate carboxylase from Cryptococcus neoformans var. grubii H99
35% identity, 6% coverage
8xl6C / Q96RQ3 Structure of human 3-methylcrotonyl-coa carboxylase at apo-state (mcc- apo)
39% identity, 11% coverage
8j78I / Q96RQ3 Human 3-methylcrotonyl-coa carboxylase in bccp-h2 state
39% identity, 10% coverage
- Ligand: 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal (8j78I)
I3LT90 Methylcrotonyl-CoA carboxylase subunit 1 from Sus scrofa
39% identity, 9% coverage
- Proteomic Profiling Reveals Specific Molecular Hallmarks of the Pig Claustrum
Pirone, Molecular neurobiology 2023 - “...0.02 1.9 665 F1RRS3 Vesicle-fusing ATPase NSF 10 7 7 83,500 6.52 0.004 2.3 665 I3LT90 Methylcrotonyl-CoA carboxylase 1 MCCC1 3 2 2 80,400 6.34 0.004 2.3 673 F1RRS3 Vesicle-fusing ATPase NSF 16 12 12 85,585 6.52 0.011 2 681 I3LJE2 Dihydropyrimidinase like 2 DPYSL2 20...”
- “...0.013 0.588 665 F1RRS3 Vesicle-fusing ATPase NSF 10 7 7 83,500 6.52 0.005 2.1 665 I3LT90 Methylcrotonyl-CoA carboxylase 1 MCCC1 3 2 2 80,400 6.34 0.005 2.1 918 F1RXD5 Copine 3 CPNE3 9 5 5 59,644 5.57 0.03 1.5 959 I3L7D3 Synapsin II SYN2 31 18...”
- An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients
Poulsen, Frontiers in molecular neuroscience 2017 - “...4,371 3,191 1,643 274 F1SGG2 Keratin 8 * 54.427 6,176 6,286 2,860 2,597 2,074 791 I3LT90 methylcrotonoyl-CoA carboxylase 1 * 80.418 2,080 909 1,427 1,133 623 478 3,089 498 3,330 481 863 498 I3LAH3 Numb-like protein * 64.815 563 240 1,876 882 551 123 2,944 507...”
W7TNH0 pyruvate carboxylase from Nannochloropsis gaditana
34% identity, 5% coverage
8xl6A / Q96RQ3 Structure of human 3-methylcrotonyl-coa carboxylase at apo-state (mcc- apo)
39% identity, 9% coverage
MCCC1 / Q96RQ3 3-methylcrotonyl-CoA carboxylase α subunit (EC 6.4.1.4) from Homo sapiens (see 4 papers)
MCCA_HUMAN / Q96RQ3 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; MCCase subunit alpha; 3-methylcrotonyl-CoA carboxylase 1; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.4 from Homo sapiens (Human) (see 15 papers)
39% identity, 9% coverage
- function: Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3- methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.
catalytic activity: 3-methyl-(2E)-butenoyl-CoA + hydrogencarbonate + ATP = 3- methyl-(2E)-glutaconyl-CoA + ADP + phosphate + H(+) (RHEA:13589)
cofactor: biotin
subunit: Probably a dodecamer composed of six biotin-containing alpha subunits (MCCC1) and six beta (MCCC2) subunits (PubMed:17360195). Interacts (via the biotin carboxylation domain) with SIRT4 (PubMed:23438705). - Aqueous Extracts of Ocimum gratissimum Sensitize Hepatocellular Carcinoma Cells to Cisplatin through BRCA1 Inhibition.
Chen, International journal of molecular sciences 2024 - “...ITPRIPL2 Inositol 1,4,5-trisphosphate receptor-interacting protein-like 2 0.01 0.01 Q9BRS8 LARP6 La-related protein 6 0.01 0.01 Q96RQ3 MCCC1 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial 0.01 0.01 Q99081 TCF12 Transcription factor 12 0.01 0.01 Q13796 SHROOM2 Protein Shroom2 0.01 0.01 Q69YH5 CDCA2 Cell division cycle-associated protein 2 0.01 0.01...”
- SAV-Pred: A Freely Available Web Application for the Prediction of Pathogenic Amino Acid Substitutions for Monogenic Hereditary Diseases Studied in Newborn Screening.
Zadorozhny, International journal of molecular sciences 2023 - “...5 58 208 271 21 15 0.703 0.707 HADHA Long-chain L-3 hydroxyacyl-CoA dehydrogenase deficiency 600890 Q96RQ3 12 9 476 497 9 11 0.813 0.808 HADHB Trifunctional protein deficiency 143450 P50747-1 2 14 309 325 17 5 0.961 0.961 HBB Hemoglobinopathies 141900 P68871 27 149 79 255...”
- Interactome Analysis of the ER Stress Sensor Perk Uncovers Key Components of ER-Mitochondria Contact Sites and Ca2+ Signalling
Sassano, Contact (Thousand Oaks (Ventura County, Calif.)) 2021 - “...27 17 100% 100% Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Homo sapiens OX=9606 GN=MCCC1 PE=1 SV=3 Q96RQ3 MCCC1 80 kDa 50 25 40 19 100% 100% Lysine-rich nucleolar protein 1 OS=Homo sapiens OX=9606 GN=KNOP1 PE=1 SV=1 Q1ED39 KNOP1 52 kDa 61 33 42 23 100% 100% Arginine...”
- Inhibition of Mitochondrial Complex Function-The Hepatotoxicity Mechanism of Emodin Based on Quantitative Proteomic Analyses
Lin, Cells 2019 - “...Process GO:0005759 mitochondrial matrix 506 30 1.5 10 16 5.7 10 16 O76031, P48735, Q9BQC6, Q96RQ3, Q9UNQ2, Q07820, Q9NYK5, Q32P41, Q6NVY1, Q8N983, Q9BZE1, Q9H2W6, Q16822, Q13084, O95182, P30038, Q8IXM3, Q969S9, Q9Y291, P11498, Q5U5X0, Q8NFF5, P82932, O00217, P82675, P28331, O75306, P42704, Q96AG4, Q13057 Cellular Component GO:0005761 mitochondrial...”
- Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls.
Berge, Clinical proteomics 2019 - “...0.87 P20645 Cation-dependent mannose-6-phosphate receptor M6PR 0.006115 0.1934 22.3302 0.22866 23.6924 0.17682 22 4 0.84 Q96RQ3 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial MCCC1 0.007633 0.5028 21.2898 0.19278 23.5502 0.34081 16.4 7 0.83 P78417 Glutathione S-transferase omega-1 GSTO1 0.007795 0.2279 23.6102 0.09562 23.1469 0.19987 59.3 14 0.79 P24666...”
- Identification of proteins interacting with the mitochondrial small heat shock protein Hsp22 of Drosophila melanogaster: Implication in mitochondrial homeostasis
Dabbaghizadeh, PloS one 2018 - “...Phosphatidyl glycerophosphatase and protein-tyrosine phosphatase1 (Q8WUK0) 0.33 0.1 0 0 Methylcrotonyl-CoA carboxylase subunit alpha, mitochondrial (Q96RQ3) 0 0 0.34 0 Isoform 3 of Carbamoyl-phosphate synthase, mitochondrial (P31327) 0 0 0.74 0.04 Pyruvate carboxylase, mitochondrial (P11498) 0 0.04 1 0.1 0.06 Hydroxysteroid dehydrogenase-like protein 2 (Q6YN16) 0.16...”
- SAMHD1 Posttranscriptionally Controls the Expression of Foxp3 and Helios in Human T Regulatory Cells.
Kim, Journal of immunology (Baltimore, Md. : 1950) 2018 - “...MCCB_HUMAN 348.2093251 (Q9HCC0) Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial Mitochondrial enzymes with Biotin cofactor MCCA_HUMAN 249.9351293 (Q96RQ3) Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Mitochondrial enzymes with Biotin cofactor PCCA_HUMAN 93.580363 (P05165) Propionyl-CoA carboxylase alpha chain, mitochondrial Mitochondrial enzymes with Biotin cofactor DLDH_HUMAN 165.104467 (P09622) Dihydrolipoyldehydrogenase, mitochondrial Photo damage-related...”
- Mitochondrial O-GlcNAc Transferase (mOGT) Regulates Mitochondrial Structure, Function, and Survival in HeLa Cells.
Sacoman, The Journal of biological chemistry 2017 - More
Pcar_1957 pyruvate carboxylase from Pelobacter carbinolicus str. DSM 2380
35% identity, 6% coverage
B6SKB7 Methylcrotonoyl-CoA carboxylase subunit alpha from Zea mays
37% identity, 9% coverage
- Leaf Proteomic Analysis in Seedlings of Two Maize Landraces with Different Tolerance to Boron Toxicity
Mamani-Huarcaya, Plants (Basel, Switzerland) 2023 - “...4 FC 3 p -Value 4 FCSA/ FCPA 5 Function/Biological Process 6 AMINO ACID METABOLISM B6SKB7 Zm00001d031013 Methylcrotonoyl-CoA carboxylase subunit 4.44 0.0022 3.56 0.0049 0.80 Leucine degradation A0A1D6K836 Zm00001d029848 Branched-chain amino-acid aminotransferase 2.35 0.0272 1.65 0.0241 0.70 Branched-chain amino acid biosynthesis B4G011 Zm00001d046923 d -3-phosphoglycerate dehydrogenase...”
- “...5 AMINO ACID AND PEPTIDE METABOLISMS Proteins very strongly induced by B toxicity in Pacha B6SKB7 Zm00001d031013 Methylcrotonoyl-CoA carboxylase subunit 4.44 0.0022 Leucine degradation Proteins very strongly repressed by B toxicity in Pacha A0A1D6ICL3 Zm00001d021596 Adenosine 5-phosphosulfate reductase-like1 0.29 0.0140 Cysteine biosynthetic process. Sulfate reduction B6TZD1...”
Q75RY4 urea carboxylase (EC 6.3.4.6) from Oleomonas sagaranensis (see paper)
35% identity, 5% coverage
TWT020 biotin carboxylase from Tropheryma whipplei str. Twist
43% identity, 9% coverage
NP_001280202 methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform 2 from Homo sapiens
39% identity, 10% coverage
- Methylcrotonyl-CoA carboxylase subunit 1 (MCCA) regulates multidrug resistance in multiple myeloma.
Feng, Life sciences 2023 (PubMed)- GeneRIF: Methylcrotonyl-CoA carboxylase subunit 1 (MCCA) regulates multidrug resistance in multiple myeloma.
- Association study of MCCC1/LAMP3 and DGKQ variants with Parkinson's disease in patients of Malay ancestry.
Lim, Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology 2021 (PubMed)- GeneRIF: Association study of MCCC1/LAMP3 and DGKQ variants with Parkinson's disease in patients of Malay ancestry.
- SNPs in SNCA, MCCC1, DLG2, GBF1 and MBNL2 are associated with Parkinson's disease in southern Chinese population.
Zhao, Journal of cellular and molecular medicine 2020 - GeneRIF: SNPs in SNCA, MCCC1, DLG2, GBF1 and MBNL2 are associated with Parkinson's disease in southern Chinese population.
- 3-Methylcrotonyl-CoA carboxylase deficiency newborn screening in a population of 536,008: is routine screening necessary?
Wang, Journal of pediatric endocrinology & metabolism : JPEM 2019 (PubMed)- GeneRIF: Mutation in the methylcrotonoyl-CoA carboxylase gene is associated with 3-Methylcrotonyl-CoA carboxylase deficiency leading to feeding difficulties and vomiting.
- [Genetic analysis of newborns with abnormal metabolism of 3-hydroxyisovalerylcarnitine].
Wu, Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences 2019 - GeneRIF: Mutations on MCCC1 and MCCC2 genes are the major genetic causes for the increased C5-OH in neonates
- Association of three candidate genetic variants in RAB7L1/NUCKS1, MCCC1 and STK39 with sporadic Parkinson's disease in Han Chinese.
Wang, Journal of neural transmission (Vienna, Austria : 1996) 2016 (PubMed)- GeneRIF: Our study provides strong support for the susceptibility role of RAB7L1/NUCKS1 rs823118 and MCCC1 rs12637471 in sporadic Parkinson's disease in a Han Chinese population
- 3-Methylcrotonyl-CoA carboxylase deficiency: Mutational spectrum derived from comprehensive newborn screening.
Fonseca, Gene 2016 (PubMed)- GeneRIF: This study reports data mainly obtained from the Portuguese newborn screening program collected over a ten-year period. Analysis of the MCCC1 and MCCC2 genes yielded 26 previously unreported mutations and a variant of clinically unknown significance.
- Methylcrotonoyl-CoA carboxylase 1 potentiates RLR-induced NF-κB signaling by targeting MAVS complex.
Cao, Scientific reports 2016 - GeneRIF: MCCC1 plays an essential role in virus-triggered, MAVS-mediated activation of NF-kappaB signaling.
- More
E9PHF7 Methylcrotonyl-CoA carboxylase subunit 1 from Homo sapiens
39% identity, 10% coverage
B9X0T8 pyruvate carboxylase (EC 6.4.1.1) from Emiliania huxleyi (see paper)
43% identity, 5% coverage
An04g02090 pyruvate carboxylase pyc-Aspergillus niger from Aspergillus niger
36% identity, 6% coverage
Q9HES8 Pyruvate carboxylase from Aspergillus niger
36% identity, 6% coverage
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory