PaperBLAST
PaperBLAST Hits for 76 a.a. (MVTPTKHAIG...)
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>76 a.a. (MVTPTKHAIG...)
MVTPTKHAIGLRSASGVELLAHIGLDTVTFDGTPFSLKVKEGDTVKKGEVLVEFDKAFIE
DIEESALHQKIFTVVS
Running BLASTp...
Found 251 similar proteins in the literature:
KP1_3804 putative cellobiose-specific PTS permease from Klebsiella pneumoniae NTUH-K2044
61% identity, 9% coverage
A6TBM5 Cellobiose-specific PTS permease from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
KPN_02578 putative cellobiose-specific PTS permease from Klebsiella pneumoniae subsp. pneumoniae MGH 78578
61% identity, 9% coverage
- Mutation of kvrA Causes OmpK35 and OmpK36 Porin Downregulation and Reduced Meropenem-Vaborbactam Susceptibility in KPC-Producing Klebsiella pneumoniae
Dulyayangkul, Antimicrobial agents and chemotherapy 2020 (secret) - Fate of the H-NS-repressed bgl operon in evolution of Escherichia coli
Sankar, PLoS genetics 2009 - “...protein, FMN-linked), KPN_02580 putative carbohydrate-selective porin (not homologous to bglH ), KPN_02579 putative transcriptional antiterminator, KPN_02578 putative cellobiose-specific PTS permease, KPN_02577 putative phospho-,D-glucosidase, KPN_02576 putative channel/filament proteins ( yohG ), as well as yieC Carbohydrate-specific outer membrane porin in cryptic operon (KPN_04128), yieL putative xylanase (KPN_04127),...”
BWI76_RS19555 Beta-glucoside phosphotransferase system, IIA/IIB/IIC components from Klebsiella michiganensis M5al
61% identity, 9% coverage
- mutant phenotype: Specific phenotype: utilization of D-Salicin and D,L-Malic Acid; the malate phenotype is unexplained
SA0183 hypothetical protein from Staphylococcus aureus subsp. aureus N315
SAV0189 PTS enzyme II from Staphylococcus aureus subsp. aureus Mu50
55% identity, 10% coverage
- Exposure of clinical MRSA heterogeneous strains to β-lactams redirects metabolism to optimize energy production through the TCA cycle
Keaton, PloS one 2013 - “...during HeR/HoR selection including putative mannose specific PTS (SA2527), glucose specific PTS ( glcA , SA0183), putative fructose specific PTS (SA2434), glucoside specific PTS ( ptsG , SA2326) and glucose specific PTS (SA1566). A group of hypothetical proteins predicted to be related to carbohydrate metabolism were...”
- Transcriptional signature following inhibition of early-stage cell wall biosynthesis in Staphylococcus aureus
O'Neill, Antimicrobial agents and chemotherapy 2009 - “...guest Downregulated genes MW0552 SA0009 SA0085 SA0100 SA0111 SA0183 SA0213 SA0268 SA0269 SA0270 SA0325 SA0423 SA0497 SA0498 SA0654 SA0655 SA0820 SA0905 SA0912...”
- Characterization of the oxygen-responsive NreABC regulon of Staphylococcus aureus
Schlag, Journal of bacteriology 2008 - “...2 mM nitrate Locus taga Upregulated genes SA0143 SA0183 SA0232 SA0477 SA0478 SA0562 SA0588 SA0589 SA1140 SA1149 SA1150 SA1183 SA1269 SA1270 SA1271 SA1506 SA1709...”
- An antibiotic that inhibits a late step in wall teichoic acid biosynthesis induces the cell wall stress stimulon in Staphylococcus aureus
Campbell, Antimicrobial agents and chemotherapy 2012 - “...cycle Fermentation Fermentation TCA cycle -4.0 SAV1199 SAV0189 SAS0665 pyrP glcA NA SAV0389 SACOL0689 pbuX NA Glucose-specific PTS transporter protein, IIABC...”
- Two novel point mutations in clinical Staphylococcus aureus reduce linezolid susceptibility and switch on the stringent response to promote persistent infection
Gao, PLoS pathogens 2010 - “...SAS0164 glucose-specific PTS transporter protein, IIABC component 0.2 SAS2090 6-phospho-beta-galactosidase 0.04 SAS2096 galactose-6-phosphate isomerase 0.01 SAV0189 glcA glucose-specific PTS enzyme II 0.2 SAV0242 maltose and glucose-specific PTS enzyme II 0.1 SAV0962 pgi glucose-6-phosphate isomerase 0.3 SAV2189 lacG 6-phospho-beta-galactosidase 0.01 SAV2190 lace PTS system, lactose-specific IIBC component...”
- Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures
Even, Applied and environmental microbiology 2009 - “...pfk pykA SAV0269 SAV0214 MW0245 MW0190 rbsD SAV2158 SAV0189 SAV2323 SAV0474 MW2084 MW0163 MW2244 MW0428 mtlA glcA glvC treP SAV0771 MW0733 gapR MW2526 MW0999...”
SAS0164 glucose-specific PTS transporter protein, IIABC component from Staphylococcus aureus subsp. aureus MSSA476
SAUSA300_0191 PTS system, glucose-specific IIBC component domain protein from Staphylococcus aureus subsp. aureus USA300_FPR3757
MW0163 ORFID:MW0163~PTS enzyme II (EC 2.7.1.69), glucose-specific, factor IIA homologue from Staphylococcus aureus subsp. aureus MW2
SACOL0175 PTS system, IIABC components from Staphylococcus aureus subsp. aureus COL
55% identity, 10% coverage
- Exposure of clinical MRSA heterogeneous strains to β-lactams redirects metabolism to optimize energy production through the TCA cycle
Keaton, PloS one 2013 - “...20.3 hypothetical protein homolog of L-lactate permease lctP SA2156 4.6 hypothetical protein Maltose/maltodextrin transport permease SAS0164 2.9 glucose-specific PTS transporter protein, IIABC component Phosphotransferase transport system (PTS), glucose-specific. SA0099 5.5 putative PTS transport system, IIABC component Putative phosphotransferase transport system (PTS), mannose specific. SAS2527 8.9 hypothetical...”
- An antibiotic that inhibits a late step in wall teichoic acid biosynthesis induces the cell wall stress stimulon in Staphylococcus aureus
Campbell, Antimicrobial agents and chemotherapy 2012 - “...SACOL2026 SA1248 SACOL2056 Transport and binding proteins SAS0164 NAc carrier protein ligase ligase subunit 2 Capsular polysaccharide synthesis enzyme...”
- Two novel point mutations in clinical Staphylococcus aureus reduce linezolid susceptibility and switch on the stringent response to promote persistent infection
Gao, PLoS pathogens 2010 - “...AND METABOLISM: Down-regulated SACOL2180 lacG 6-phospho-beta-galactosidase 0.03 SAR2281 lacE PTS system, lactose-specific IIBC component 0.4 SAS0164 glucose-specific PTS transporter protein, IIABC component 0.2 SAS2090 6-phospho-beta-galactosidase 0.04 SAS2096 galactose-6-phosphate isomerase 0.01 SAV0189 glcA glucose-specific PTS enzyme II 0.2 SAV0242 maltose and glucose-specific PTS enzyme II 0.1 SAV0962...”
- Impact of the pentose phosphate pathway on metabolism and pathogenesis of Staphylococcus aureus
Kim, PLoS pathogens 2023 (no snippet) - Glucose Augments Killing Efficiency of Daptomycin Challenged Staphylococcus aureus Persisters
Prax, PloS one 2016 - “...the glucose-PTS specific IIABC component, SAUSA300_2476 (NE39) and the glucose-PTS specific IIBC component domain protein, SAUSA300_0191 (NE172). Compared to our PTS-positive control strain NE1046 ( fdhD , formate dehydrogenase, SAUSA300_2231), both PTS mutants killing curves showed a less pronounced incongruity between challenge with Glc-DAP and DAP...”
- Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection
Vitko, mBio 2016 - “...material). Interestingly, 4 of the 10 unique S.aureus alleles are predicted to encode glucose transporters: SAUSA300_0191 ( glcA ), SAUSA300_0194, SAUSA300_0236 ( glcC ), and SAUSA300_0259 (see TableS1 ). Importantly, glucose is (i) largely absent from the skin surface, (ii) the most abundant free carbohydrate in...”
- Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures
Even, Applied and environmental microbiology 2009 - “...MW0245 MW0190 rbsD SAV2158 SAV0189 SAV2323 SAV0474 MW2084 MW0163 MW2244 MW0428 mtlA glcA glvC treP SAV0771 MW0733 gapR MW2526 MW0999 MW0586 MW0588 mqo2 ctaB...”
- Virulence and Metabolism
Richardson, Microbiology spectrum 2019 (secret) - Changes in the Staphylococcus aureus transcriptome during early adaptation to the lung
Chaffin, PloS one 2012 - “...6 hours in the lung, respectively. Also showing a similar pattern of expression were glcA (SACOL0175) and a putative glucose uptake permease (SACOL2246). That these were the only sugar uptake systems significantly increased in vivo is consistent with glucose as the primary carbohydrate utilized in vivo....”
- The Staphylococcus aureus LytSR two-component regulatory system affects biofilm formation
Sharma-Kuinkel, Journal of bacteriology 2009 - “...SACOL1262 SACOL1782 SACOL2198 SACOL2199 SACOL2553 SACOL2146 SACOL2149 SACOL0175 SACOL0543 Fold downregulation Description VOL. 191, 2009 REGULATION OF S. AUREUS...”
- “...SACOL2283 SACOL2284 SACOL2563 SACOL0247 SACOL0248 SACOL2554 SACOL0175 SACOL2146 SACOL1305 SACOL2072 Fold downregulation Ribosomal protein L5 Ribosomal protein...”
- A defect in menadione biosynthesis induces global changes in gene expression in Staphylococcus aureus
Kohler, Journal of bacteriology 2008 - “...and fructose (SACOL2663) and also of glucose (ptsI, SACOL0175, SACOL2552) (see Table S2 in the supplemental material). Very recently, it was shown that the...”
RDJ18_RS00835 glucose-specific PTS transporter subunit IIBC from Staphylococcus aureus
SAR0190 glucose-specific PTS transporter protein, IIABC component from Staphylococcus aureus subsp. aureus MRSA252
55% identity, 10% coverage
- Multiomics analysis of Staphylococcus aureus ST239 strains resistant to virulent Herelleviridae phages
Kornienko, Scientific reports 2024 - “...the transport of glucose and fructose into the cell at both early and late stages (RDJ18_RS00835, RDJ18_RS01075, RDJ18_RS01200, RDJ18_RS03920, RDJ18_RS13495, RDJ18_RS14335) (Table S4 ). The key genes encoding enzymes involved in glycolysis exhibited upregulation: pyruvate kinase (RDJ18_RS06570), L-lactate dehydrogenase (RDJ18_RS01065), phosphoglycerate kinase (RDJ18_RS04310), triose-phosphate isomerase (RDJ18_RS04315),...”
- The Staphylococcus aureus response to unsaturated long chain free fatty acids: survival mechanisms and virulence implications
Kenny, PloS one 2009 - “...regulator ( agr ) locus 10.20 1.21E-05 Metabolism SAR0150 adhE putative aldehyde-alcohol dehydrogenase 2.25 1.67E-02 SAR0190 glcA glucose-specific PTS transporter protein, IIABC component 2.05 3.76E-02 SAR0829 pgk phosphoglycerate kinase 2.76 2.16E-03 SAR0830 tpiA triosephosphate isomerase 2.75 1.69E-03 SAR0831 pgm putative phosphoglycerate mutase 2.83 2.22E-03 SAR0832 eno...”
Q831B4 PTS system, beta-glucoside-specific IIABC component from Enterococcus faecalis (strain ATCC 700802 / V583)
EF2598 PTS system, beta-glucoside-specific IIABC component from Enterococcus faecalis V583
60% identity, 9% coverage
- Gliotoxin-mediated bacterial growth inhibition is caused by specific metal ion depletion
Downes, Scientific reports 2023 - “...ligase Unique N/A 3 13.3 Q830C2 PTS system, beta-glucoside-specific IIABC component Unique N/A 3 6.9 Q831B4 Uncharacterized protein Unique N/A 2 55.9 Q834Y7 Histidine kinase Unique N/A 3 9.7 Q837B6 Abhydrolase_3 domain-containing protein Unique N/A 3 14.3 Q838Q5 Glyoxylase family protein* Unique N/A 3 11.2 Q834I3...”
- “...3 Absent N/A 2 26.5 P59628 PTS system, beta-glucoside-specific IIABC component Absent N/A 5 15.2 Q831B4 6-aminohexanoate-cyclic-dimer hydrolase, putative Absent N/A 10 19.1 Q836S5 Prephenate dehydrogenase Absent N/A 7 23.4 H7C6X1 Mga domain-containing protein Absent N/A 6 15.6 Q82ZN7 Protease synthase and sporulation negative regulatory protein...”
- Genomic Landscape of Multidrug Resistance and Virulence in Enterococcus faecalis IRMC827A from a Long-Term Patient
Borgio, Biology 2023 - “...29375870 c EF1302 Transcriptional regulator 100 100 99 1 10 167 28 Victors 29377084 c EF2598 PTS system, beta-glucoside-specific IIB component/PTS system, beta-glucoside-specific IIC component/PTS system, beta-glucoside-specific IIA component 100 100 99 0.0 29 Victors 29377078 c EF2591 Glyoxalase family protein 100 97 100 1 10...”
- Functional studies of E. faecalis RNase J2 and its role in virulence and fitness
Gao, PloS one 2017 - “...protein 2.56 0.013 ef2162 tRNA transferase 3.85 0.022 ef2597 glycosyl hydrolase, family 1 3.33 0.025 ef2598 PTS system component 3.57 0.048 ef2599 transcription antiterminator, BglG family 2.56 0.044 ef2601 conserved hypothetical protein 2.44 0.013 a EF numbers and the encoded protein are from the V583 genome...”
SXYL_01421 PTS sugar transporter subunit IIA from Staphylococcus xylosus
50% identity, 43% coverage
- Insight into the Genome of Staphylococcus xylosus, a Ubiquitous Species Well Adapted to Meat Products
Leroy, Microorganisms 2017 - “...Glucose SXYL_00369 ptsG EIICBA SXYL_00698 gdh Glucose 1-dehydrogenase SXYL_00253 EIIBC SXYL_00699 glcU Glucose uptake protein SXYL_01421 crr EIIA SXYL_01308 glkA Glucokinase Sucrose Lactate SXYL_00555 scrA EIIBC SXYL_00250 Lactate permease SXYL_00886 scrR Repressor SXYL_00577 Lactate permease SXYL_00887-88 scrBK Hydrolase, Fructokinase SXYL_00170 lactate-quinone oxidoreductase others Lactose SXYL_00060, SXYL_00626...”
LSEI_2700 Phosphotransferase system IIA component from Lactobacillus casei ATCC 334
51% identity, 45% coverage
- Analysis of gene expression profiles of Lactobacillus paracasei induced by direct contact with Saccharomyces cerevisiae through recognition of yeast mannan
Yamasaki-Yashiki, Bioscience of microbiota, food and health 2017 - “...4.3 (41) 1.6 LSEI_0079 Hypothetical protein Membrane 1.6 4.5 (9) 5.5 (18) 4.9 (23) 1.2 LSEI_2700 Phosphotransferase system IIA component Intracellular 1.6 4.3 (10) 6.5 (10) 5.7 (11) 1.6 LSEI_0059 Hypothetical protein Membrane 1.4 4.2 (11) 6.5 (9) 4.9 (22) 1.5 LSEI_0294 ABC-type cobalt transport system,...”
- Effect of periodontal pathogens on the metatranscriptome of a healthy multispecies biofilm model
Frias-Lopez, Journal of bacteriology 2012 - “...casei and Streptococcus mitis were highly downregulated (LSEI_2700, LSEI_0370, LSEI_2073, SMT1148, SMT0181, SMT1149, SMT1765, and SMT0182). In the pathogenic...”
Ent638_2740 beta-glucoside-specific PTS system components IIABC from Enterobacter sp. 638
56% identity, 9% coverage
- Fate of the H-NS-repressed bgl operon in evolution of Escherichia coli
Sankar, PLoS genetics 2009 - “...For Enterobacter : Ent638_2738 RND efflux system, outer membrane lipoprotein, Ent638_2739 glycoside hydrolase, family 1, Ent638_2740 PTS system, -glucoside-specific IIABC, Ent638_2741 transcriptional antiterminator, BglG, and Ent638_2742 dihydrouridine synthase, DuS. For Erwinia ECA1868 hypothetical protein, arbG -glucoside operon antiterminator (ECA1869), arbF -glucoside-specific PTS system components IIABC (ECA1870),...”
Cbei_3273 PTS system, beta-glucoside-specific IIABC subunit from Clostridium beijerincki NCIMB 8052
56% identity, 10% coverage
SA1255 PTS system, glucose-specific enzyme II, A component from Staphylococcus aureus subsp. aureus N315
SAV1422 glucose-specific enzyme II, PTS system A component from Staphylococcus aureus subsp. aureus Mu50
50% identity, 43% coverage
- VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus
Boyle-Vavra, Antimicrobial agents and chemotherapy 2013 - “...Folding, sorting, and degradation Peptidoglycan synthesis SAUSA300_1315 SA1255 Kuroda et al. (9) S. aureus locus no. Glycolysis and starch metabolism a...”
- Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
Anderson, FEMS immunology and medical microbiology 2010 - “...* 3.8 2.5 2.5 rbgA SA1260 ribosomal biogenesis GTPase sa_c1294s1067_a_at * 5.3 2.5 2.5 rimM SA1255 16S rRNA processing protein sa_c6841s5976_a_at 3.9 2.5 2.5 rnpA SA2739 ribonuclease P protein sa_c7620s6631_a_at * 4.3 15 stable rplA SA0584 50S ribosomal protein L1 sa_c9959s8654_a_at * 3.4 15 stable rplB...”
- “...* 3.8 2.5 2.5 rbgA SA1260 ribosomal biogenesis GTPase sa_c1294s1067_a_at * 11.1 2.5 5 rimM SA1255 16S rRNA processing protein sa_c2422s2004_a_at 3.8 2.5 2.5 rluB SA1536 ribosomal large subunit pseudouridine synthase B sa_c7620s6631_a_at * 6.5 15 15 rplA SA0584 50S ribosomal protein L1 sa_c9959s8654_a_at * 5.8...”
- Transcriptional signature following inhibition of early-stage cell wall biosynthesis in Staphylococcus aureus
O'Neill, Antimicrobial agents and chemotherapy 2009 - “...SA1228 SA1229 SA1230 SA1231 SA1235 SA1236 SA1238 SA1254 SA1255 SA1256 SA1257 SA1532 SA1545 SA1546 SA1691 SA1858 SA1859 SA1860 SA1861 SA1862 SA1863 SA1864 SA1865...”
- Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus
Donat, Journal of bacteriology 2009 - “...3.1 2.9 Phosphotransferase system SA0186 SA0318 SA0320 SA1255 SA2114 glvC 2.8 2.0 1.7 Membrane bioenergetics (electron transport chain and ATP synthase)...”
- The lantibiotic mersacidin is a strong inducer of the cell wall stress response of Staphylococcus aureus
Sass, BMC microbiology 2008 - “...4.9 3.2 6.2 SA1183 opuD glycine betaine transporter Protein transport and binding 0.9 0.5 0.5 SA1255 PTS system, glucose-specific enzyme IIA component Protein transport and binding 4.7 10.7 2.6 13.1 SA1282 recU recombination protein U homologue DNA recombination 3.6 2.5 2.2 2.5 SA1283 pbp2 penicillin-binding protein...”
- Mapping the pathways to staphylococcal pathogenesis by comparative secretomics
Sibbald, Microbiology and molecular biology reviews : MMBR 2006 - “...57651702 24636605d Protein Function CitB CspA OdhA SA1255 Aconitate hydratase Major cold shock protein CspA 2-Oxoglutarate dehydrogenase E1 PTS system,...”
- Transcriptomic Analysis of the Activity of a Novel Polymyxin against Staphylococcus aureus
Zhao, mSphere 2016 - “...be under the control of the VraSR two-component system. These included hypothetical proteins SAV1424, SAV1423, SAV1422, SAV1421, and SAV2556, which were upregulated 3.50-, 3.90-, 3.73-, 3.50-, and 4.28-fold, respectively, hypothetical membrane protein SAV1650, which was upregulated 3.54-fold, and a peptidyl-prolyl isomerase, prsA , which was upregulated...”
- The Quorum Sensing Inhibitor Hamamelitannin Increases Antibiotic Susceptibility of Staphylococcus aureus Biofilms by Affecting Peptidoglycan Biosynthesis and eDNA Release
Brackman, Scientific reports 2016 - “...cells treated with a combination of VAN and HAM ( Supplementary Figure S6 ). Moreover, SAV1422, encoding a glucose specific enzyme was 1.80 and 3.68 fold upregulated in the presence of VAN alone or in combination with HAM. Similarly, several genes including sgtA , sgtB and...”
- The SAV1322 gene from Staphylococcus aureus: genomic and proteomic approaches to identification and characterization of gene function
Kim, BMC microbiology 2016 - “...845 citC SAV1694 Down A286 gi|15924412 Glucose-specific enzyme II, PTS system A component 297 crr SAV1422 Down A287 gi|15924701 Acetate kinase 750 ackA SAV1711 Up Energy metabolism A029 gi|15925597 Malate:quinone oxidoreductase 1353 mqo2 SAV2607 Down A055 gi|15923231 L-lactate dehydrogenase 298 lctE SAV0241 Down A056 gi|15923246 Xylitol...”
SH1484 phosphotransferase system enzyme IIA-like protein from Staphylococcus haemolyticus JCSC1435
47% identity, 43% coverage
PTW3C_BACSU / P39816 PTS system glucosamine-specific EIICBA component; EC 2.7.1.- from Bacillus subtilis (strain 168) (see 4 papers)
TC 4.A.1.1.6 / P39816 The glucosamine IICBA porter (GamP) (40% identical to 4.A.1.1.2) (Plumbridge 2015). The IIA domain in this protein can transfer the phosphoryl moiety to the maltose, N-acetylglucosamine, sucrose and trehalose PTS systems (MalP, NagP, SacP and TreP, respectively) from Bacillus subtilis (see 3 papers)
BSU02350 phosphotransferase system (PTS) glucosamine-specific enzyme IICBA component from Bacillus subtilis subsp. subtilis str. 168
47% identity, 12% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (By similarity). This system is involved in glucosamine transport (PubMed:10627040, PubMed:23667565). In vitro, when expressed in the absence of GamR and NagP, can transport N-acetylglucosamine (PubMed:23667565).
function: In addition, plays an important role in the phosphorylation of EIIA-deficient PTS transporters (PubMed:30038046). The EIIA domain can transfer a phosphoryl group to EIIA-deficient PTS transporters, enabling growth with maltose, N-acetylglucosamine, sucrose or trehalose as the sole carbon source (PubMed:30038046).
catalytic activity: D-glucosamine(out) + N(pros)-phospho-L-histidyl-[protein] = D- glucosamine 6-phosphate(in) + L-histidyl-[protein] (RHEA:37359)
disruption phenotype: Reizer et al. found that mutation of the gene results in impaired growth in the presence of glucosamine (PubMed:10627040). However, Gaugue et al. found that deletion of the gene has only a minimal effect on growth on either glucosamine or N- acetylglucosamine (PubMed:23667565). The gamP-nagP double mutant can still grow normally on glucosamine, but the gamP-ptsG double mutant grows slower on glucosamine (PubMed:23667565). - substrates: Glucosamine
- rocF affects the production of tetramethylpyrazine in fermented soybeans with Bacillus subtilis BJ3-2
Liu, BMC biotechnology 2022 - “...Up BSU02360 243.519 638.924 1.39 7.36E-04 2.77E-02 Up BSU40950 51.79 134.426 1.37 1.30E-03 4.18E-02 Up BSU02350 68.903 174.218 1.34 9.74E-04 3.42E-02 Up BSU31050 64.173 161.213 1.33 1.13E-03 3.79E-02 Up BSU11220 71.588 176.107 1.3 1.30E-03 4.18E-02 Up BSU15510 1928.76 14.424 7.05 1.22E-13 2.22E-10 Down BSU15520 2240.655 18.756...”
LBA0609 PTS enzyme II, ABC component from Lactobacillus acidophilus NCFM
53% identity, 36% coverage
- Transcriptional analysis of prebiotic uptake and catabolism by Lactobacillus acidophilus NCFM
Andersen, PloS one 2012 - “...-1,6 linked glucosides are phosphorylated concomitant with their transport by the PTS EIIABC (LBA0606 and LBA0609) permease, and that these phosphorylated disaccharides are hydrolyzed by a specific intracellular (predicted by SignalP [30] ) GH4 disaccharide 6-phospho--glucosidase into glucose-6-phosphate and either glucose from isomaltose or fructose from...”
- “...(LBA0606), a transcriptional regulator (LBA0607), a putative transporter associated protein (LBA0608) and PTS EIIA component (LBA0609) showed consistent high expression of the full locus indicating a functional connection of LBA0608 and PTS permease uptake. Values are given as the mean value () of the technical replicates...”
SPy2097 putative PTS system enzyme II from Streptococcus pyogenes M1 GAS
49% identity, 11% coverage
- Adaptation of group A Streptococcus to human amniotic fluid
Sitkiewicz, PloS one 2010 - “...system repressor M28_Spy1668 SPy1972 pulA 5.30 Pullulanase M28_Spy1767 SPy2096 dexS 6.18 39.01 Trehalose-6-phosphate hydrolase M28_Spy1768 SPy2097 - 8.81 43.06 PTS system, trehalose-specific IIBC component Values represent fold change in expression in AF at ML, LL, and S growth phases compared to expression in THY medium. Another...”
SAR1435 PTS system, glucose-specific IIA component from Staphylococcus aureus subsp. aureus MRSA252
49% identity, 43% coverage
- Altered quorum sensing and physiology of Staphylococcus aureus during spaceflight detected by multi-omics data analysis
Hauserman, NPJ microgravity 2024 - “...glucose in culture supernatants (Supplemental Table 6 ), decreased abundance of a glucose import protein (SAR1435), and functional enrichment (Table 1 ) and upregulated expression of genes and/or proteins of several sugar transporters (SAR0235, SAR0193, SAR2244, SAR1803) (Supplemental Tables 1 3 ). Several members of TCA...”
- Global network analysis of drug tolerance, mode of action and virulence in methicillin-resistant S. aureus
Overton, BMC systems biology 2011 - “...stressed cells. Indeed, several glucose catabolism and uptake proteins were upregulated, including EIIA-Glc (encoded by SAR1435, 2.65-fold), PGK (encoded by SAR0829, 1.13-fold) an intermodular hub, and PGI (encoded by SAR0924, 1.22-fold) substantiating upregulation of glycolysis upon ranalexin exposure. Interestingly, L-LDH1 (encoded by SAR0234, 0.77-fold) was RanaDown;...”
EF_0270 beta-glucoside-specific PTS transporter subunit IIABC from Enterococcus faecalis V583
51% identity, 12% coverage
SPy1986 putative PTS system, enzyme II, A component from Streptococcus pyogenes M1 GAS
49% identity, 10% coverage
- Surface export of GAPDH/SDH, a glycolytic enzyme, is essential for Streptococcus pyogenes virulence
Jin, mBio 2011 - “...). In particular, multiple operons ( SPy1057-1060 , SPy1294-1296 , SPy1299-1301 , SPy1306 , and SPy1986 ) containing genes for utilization/transport of nonglucose substrates, such as maltose/maltodextrin, mannose, mannose/fructose, N -acetylneuraminate, -glucoside, fructose, cellobiose, galactose, lactose, and trehalose were downregulated (8- to 32-fold or 2 to...”
- The role of complex carbohydrate catabolism in the pathogenesis of invasive streptococci
Shelburne, Trends in microbiology 2008 - “...Streptococcus Expression microarray Human blood [13] spy1986 (maltose transport) malE (maltodextrin-binding protein) amyA (-amylase) spy1592 (sugar transport)...”
- Central role of a bacterial two-component gene regulatory system of previously unknown function in pathogen persistence in human saliva
Shelburne, Proceedings of the National Academy of Sciences of the United States of America 2005 - “...transport and utilization of simple sugars such as glucose (spy1986). Taken together, these data strongly suggest that one of the major roles of the SptRS TCS...”
- Multilocus analysis of extracellular putative virulence proteins made by group A Streptococcus: population genetics, human serologic response, and gene transcription
Reid, Proceedings of the National Academy of Sciences of the United States of America 2001 - “...as follows: spy2211, 1,845,056-1,847,632; spy1986, 1,654,795-1,656,981; spy1972, 1,639,165-1,642,662; spy1858, 1,541,907-1,544,189; spy1857,...”
- “...CAC20606). bExpression cExpression number assigned in GenBank, e.g., spy1986, spy1857, and so forth. One gene was selected for study because it encodes a...”
abgF / AAC05713.1 PTS-dependent enzyme II from Clostridium longisporum (see paper)
44% identity, 11% coverage
SAUSA300_1315 PTS system, glucose-specific IIA component from Staphylococcus aureus subsp. aureus USA300_FPR3757
SACOL1457 PTS system, IIA component from Staphylococcus aureus subsp. aureus COL
49% identity, 43% coverage
- VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus
Boyle-Vavra, Antimicrobial agents and chemotherapy 2013 - “...Hypothetical Folding, sorting, and degradation Peptidoglycan synthesis SAUSA300_1315 SA1255 Kuroda et al. (9) S. aureus locus no. Glycolysis and starch...”
- A defect in menadione biosynthesis induces global changes in gene expression in Staphylococcus aureus
Kohler, Journal of bacteriology 2008 - “...family protein; SACOL1307, hypothetical protein; SACOL1457, PTS system, IIA component; SACOL1509, nucleoside diphosphate kinase; SACOL1555, peptidase,...”
TC 4.A.1.1.12 / Q48WG5 Maltose/Maltotriose PTS transporter, MalT (Shelburne et al., 2008) 631aas (68% identical to 4.A.1.1.11 from S. mutans from Streptococcus pyogenes serotype M1 (see paper)
M5005_Spy_1692 PTS system, glucose-specific IIABC component from Streptococcus pyogenes MGAS5005
49% identity, 11% coverage
M28_Spy1768 PTS system, trehalose-specific IIBC component from Streptococcus pyogenes MGAS6180
55% identity, 9% coverage
- Adaptation of group A Streptococcus to human amniotic fluid
Sitkiewicz, PloS one 2010 - “...phosphotransferase system repressor M28_Spy1668 SPy1972 pulA 5.30 Pullulanase M28_Spy1767 SPy2096 dexS 6.18 39.01 Trehalose-6-phosphate hydrolase M28_Spy1768 SPy2097 - 8.81 43.06 PTS system, trehalose-specific IIBC component Values represent fold change in expression in AF at ML, LL, and S growth phases compared to expression in THY medium....”
llmg_1045 similar to PTS system, beta-glucosides specific enzyme IIABC from Lactococcus lactis subsp. cremoris MG1363
47% identity, 11% coverage
P42015 PTS system glucose-specific EIICBA component (Fragment) from Geobacillus stearothermophilus
47% identity, 22% coverage
- Evolving antibody response to SARS-CoV-2 antigenic shift from XBB to JN.1.
Jian, Nature 2025 - “...quantified using a Nanodrop (Thermo Fisher, 840-317400), and the purity was confirmed by SDSPAGE (LabLead, P42015). Enzyme-linked immunosorbent assays SARS-CoV-2 XBB.1.5, HK.3 and JN.1 RBD were individually aliquoted into a 96-well plate, which was then incubated overnight at 4C. The plate was then washed three times...”
SXYL_00528 beta-glucoside-specific PTS transporter subunit IIABC from Staphylococcus xylosus
47% identity, 10% coverage
- Insight into the Genome of Staphylococcus xylosus, a Ubiquitous Species Well Adapted to Meat Products
Leroy, Microorganisms 2017 - “...Hydrolase, Fructokinase SXYL_00170 lactate-quinone oxidoreductase others Lactose SXYL_00060, SXYL_00626 Arbutin, EIIBC SXYL_00082 lacR Transcription activator SXYL_00528 Beta-glucoside, EIIABC SXYL_00083-84 lacPH Permease, Beta-galactosidase SXYL_00257-60 Cellobiose, EIIC, EIIBA SXYL_00671 galR Transcriptional regulator SXYL_02148-50 fruAK Fructose, EIIABC, catabolism, repression SXYL_00672-74 galKET Galactokinase, epimerase, P-uridylyltransferase SXYL_00277-78 Fructose, regulation, EII SXYL_00773-76...”
ECA1870 PTS system, beta-glucoside-specific IIABC component from Erwinia carotovora subsp. atroseptica SCRI1043
55% identity, 9% coverage
- Fate of the H-NS-repressed bgl operon in evolution of Escherichia coli
Sankar, PLoS genetics 2009 - “...Erwinia ECA1868 hypothetical protein, arbG -glucoside operon antiterminator (ECA1869), arbF -glucoside-specific PTS system components IIABC (ECA1870), arbB 6-phospho-,D-glucosidase (ECA1871), ECA1872 putative porin, ECA1873 putative glycosyl hydrolase exoenzyme, and ECA1874 putative inosine-uridine preferring nucleoside hydrolase. In contrast to the bgl operon genes, no orthologs for genes yieI...”
CTK_C20580 glucose PTS transporter subunit IIA from Clostridium tyrobutyricum
50% identity, 9% coverage
SAG0192 PTS system, IIABC components from Streptococcus agalactiae 2603V/R
ID870_08455 PTS system trehalose-specific EIIBC component from Streptococcus agalactiae CJB111
53% identity, 9% coverage
- Adaptive response of Group B streptococcus to high glucose conditions: new insights on the CovRS regulation network
Di, PloS one 2013 - “...sources, such as sag1805 , sag1813 , sag1814 , sag1948 1951 , sag1898 1902 and sag0192 were found to be highly down-regulated (up to 120-fold). Host-pathogen interaction genes Pathogenic bacteria, by modulating the expression of surface-associated or secreted virulence factors, can adapt to host conditions and...”
- Identification of a DNA-cytosine methyltransferase that impacts global transcription to promote group B streptococcal vaginal colonization
Manzer, mBio 2023 - “...genes that were most dysregulated were those involved in sugar metabolism or transport, including treP/C (ID870_08455, ID870_08450) , dhaK/L/M (ID870_01490ID870_01480) , lrgA/B (ID870_08490, ID870_08485) , lacA/B (ID870_00285, ID870_00290) , argF (ID870_10075) , arcC (ID870_10080) , and multiple genes belonging to pts sugar transporters. At the early...”
- “...relative to WT Locus tag Gene name Description EXP fold change ES fold change Metabolism ID870_08455 treP Trehalose phosphorylase 69.17 2.75 ID870_08450 treC Trehalose-6-phosphate hydrolase 66.48 3.00 ID870_00285 lacA Galactose-6-phosphate subunit A 4.12 5.40 ID870_00290 lacB Galactose-6-phosphate subunit B 3.42 2.82 ID870_01490 dhaK Dihydroxyacetone kinase subunit...”
SAK_0257 PTS system, trehalose-specific IIBCA component from Streptococcus agalactiae A909
53% identity, 9% coverage
- The Role of Regulator Catabolite Control Protein A (CcpA) in Streptococcus agalactiae Physiology and Stress Response
Roux, Microbiology spectrum 2022 - “...belonging to a ribose operon, SAK_0528 and SAK_0529 belonging to a galactitol PTS operon, and SAK_0257 coding a trehalose PTS. In order to demonstrate a glucose effect in S. agalactiae , the A909WT strain was cultured in chemically defined medium supplemented with different carbon sources (glucose,...”
- “...putative cre site of three genes regulated by CcpA ( rbsR , typA , and SAK_0257 encoding the trehalose phosphotransferase system) under the transcriptome conditions. Proteins encoded by rbsR and SAK_0257 are both involved in carbon metabolism. The gene typA codes for a translational regulator involved...”
- A Vaginal Tract Signal Detected by the Group B Streptococcus SaeRS System Elicits Transcriptomic Changes and Enhances Murine Colonization
Cook, Infection and immunity 2018 - “...and no. (strain A909) Phosphotransferase systems sak_0257 sak_0261 sak_0354 sak_0398 sak_0399 sak_0400 sak_0524 sak_0525 sak_0526 sak_0528 sak_0529 sak_0530...”
- “...sak_0398 sak_0399 sak_0400 sak_1920 sak_1702 sak_0354 sak_0257 Fatty acid biosynthesis and metabolism sak_0416 sak_0417 sak_0418 sak_0419 sak_0420 sak_0421...”
- The sensor histidine kinase RgfC affects group B streptococcal virulence factor expression independent of its response regulator RgfA
Gendrin, Infection and immunity 2015 - “...SAK_0398, SAK_0400, SAK_0523, SAK_ 0529, SAK_0915, SAK_0530, SAK_0257, and SAK_1920). Increased expression of such genes may facilitate GBS survival and...”
SGO_1653 trehalose PTS enzyme II from Streptococcus gordonii str. Challis substr. CH1
52% identity, 9% coverage
SPSF3K_00506 PTS transporter subunit IIBC from Streptococcus parauberis
52% identity, 8% coverage
- Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum
Lee, PloS one 2021 - “...Protein-N(pi)-phosphohistidinesugar phosphotransferase - -4.1 -3.6 G srlB SPSF3K_00184 Protein-N(pi)-phosphohistidinesugar phosphotransferase - -3.4 -3.3 G ptsG SPSF3K_00506 Protein-N(pi)-phosphohistidinesugar phosphotransferase -1.0 -3.1 -2.7 G -, Not significant (|fold change| > 1.5 and FDR < 1e-5). * eggNOG categories: V, Defense mechanism; O, Posttranslational modification, protein turnover, chaperones; K,...”
- “...fish serum ( Table 2 and S3 Table ). For instance, a gene encoding PtsG (SPSF3K_00506), a glucose-specific PTS component, was down-regulated (~ 1.9-, 8.5-, and 6.4-fold change at 1, 2 and 4 h, respectively) in the presence of fish serum ( Table 2 and S3...”
SXYL_00369 glucose-specific PTS transporter subunit IIBC from Staphylococcus xylosus
47% identity, 11% coverage
BC4050 PTS system, glucose-specific IIABC component from Bacillus cereus ATCC 14579
44% identity, 10% coverage
GBAA4269 PTS system, glucose-specific IIABC component from Bacillus anthracis str. 'Ames Ancestor'
44% identity, 10% coverage
EF1516 PTS system, IIABC components from Enterococcus faecalis V583
EF_RS07325 N-acetylglucosamine-specific PTS transporter subunit IIBC from Enterococcus faecalis V583
48% identity, 9% coverage
- The NagY regulator: A member of the BglG/SacY antiterminator family conserved in Enterococcus faecalis and involved in virulence
Soussan, Frontiers in microbiology 2022 - “...with BglG from E . coli ( blastP alignment). This gene is followed by the ef1516 (EF_RS07325) gene ( Figure 2 ), recently renamed nagE on V583 genome, encoding a NAG specific EIICBA PTS transporter ( Keffeler et al., 2021b ). Thus, the NagY and NagE...”
- Metabolism of Poly-β1,4-N-Acetylglucosamine Substrates and Importation of N-Acetylglucosamine and Glucosamine by Enterococcus faecalis
Keffeler, Journal of bacteriology 2021 (secret) - Influence of the Alternative Sigma Factor RpoN on Global Gene Expression and Carbon Catabolism in Enterococcus faecalis V583
Keffeler, mBio 2021 - “...contributed to glucose uptake in E. faecalis . We therefore constructed a deletion mutant of ef1516 and assessed its growth in CDM supplemented with either 10mM or 100mM glucose (see Fig.S2 in the supplemental material). EF1516 shares approximately 39% amino acid sequence identity and 56% sequence...”
- “...source in E. faecalis , as there was no significant difference in growth between the ef1516 mutant and the parental V583 strain ( Fig.S2 ). FIG1 Growth of Enterococcus faecalis in chemically defined medium (CDM) with the sugars indicated as the principal carbon sources: (A) glucose,...”
- The transcriptome of the nosocomial pathogen Enterococcus faecalis V583 reveals adaptive responses to growth in blood
Vebø, PloS one 2009 - “...54 dependent PRD controlled PTS systems, mphAD (EF1953-50) [57] , and a putative N-acetylglucosamine PTS (EF1516), were down-regulated in YTB and blood respectively. Also, the mpoAD PTS system (EF2980-76) [57] was found to be up-regulated and appeared to be co-regulated with EF2982-81. Similarly, the inferred sorbitol...”
- Transcriptional response of Enterococcus faecalis V583 to erythromycin
Aakra, Antimicrobial agents and chemotherapy 2005 - “...EF2852 EF3272 EF0021 EF0022 EF0717 EF0958 EF1051 EF1261 EF1516 EF2978 EF2979 EF0846 EF1170 EF3214 EF0188 EF0636 EF0744 EF0760 EF0789 EF0790 EF0872 EF0941 EF1103...”
- The NagY regulator: A member of the BglG/SacY antiterminator family conserved in Enterococcus faecalis and involved in virulence
Soussan, Frontiers in microbiology 2022 - “...BglG from E . coli ( blastP alignment). This gene is followed by the ef1516 (EF_RS07325) gene ( Figure 2 ), recently renamed nagE on V583 genome, encoding a NAG specific EIICBA PTS transporter ( Keffeler et al., 2021b ). Thus, the NagY and NagE proteins...”
D8IBR5 PTS system, glucose subfamily, IIA subunit from Brachyspira pilosicoli (strain ATCC BAA-1826 / 95/1000)
51% identity, 9% coverage
- The Exposed Proteomes of Brachyspira hyodysenteriae and B. pilosicoli
Casas, Frontiers in microbiology 2016 - “...suhB C Unchar D8IEP8 90 D8IEP8 BP951000_1640 BRAPL PTS system glucose subfamily IIA subunit 90 D8IBR5 ptsG BRAPL PTS system fructose-specific IIABC component 88 D8ID87 fruA BRAPL Transcriptional regulator XRE family 86 J9TSW8 B2904_orf609 BRAPL PTS system fructose specific transporter subunit IIABC 83 K0JLH4 WESB_2470 BRAPL...”
SMU_2038, SMU_RS09325 PTS system trehalose-specific EIIBC component from Streptococcus mutans UA159
47% identity, 11% coverage
- A strain of <i>Streptococcus mitis</i> inhibits biofilm formation of caries pathogens via abundant hydrogen peroxide production
Williams, Applied and environmental microbiology 2025 - “...biosynthesis, bacteriocins, as well as PTS systems for fructose (SMU_872), fructose/mannose (SMU_1956cSMU_1961c), trehalose (SMU_2037 and SMU_2038), and manLMN ( Fig. 7H ). Fig 7 Transcriptomes of S. mutans and S. mitis during coculture growth. ( A ) Principal component analysis (PCA) from RNA-Seq expression data (...”
- Identification of the antibacterial action mechanism of diterpenoids through transcriptome profiling
Moon, Frontiers in microbiology 2022 - “...IICBA mtlA1 3.34 1.65 1.41 SMU_RS01555 PTS glucitol/sorbitol transporter subunit IIA 0.88 1.68 1.79 1.85 SMU_RS09325 PTS system trehalose-specific EIIBC component pttB 2.94 2.56 2.43 SMU_RS04080 Extracellular solute-binding protein msmE 1.23 0.86 2.31 2.26 SMU_RS04095 Sucrose phosphorylase gtfA 0.98 1.69 1.71 SMU_RS04105 Alpha-glucosidase dexB 1.28 1.23...”
- Transcriptional Profiling Reveals the Importance of RcrR in the Regulation of Multiple Sugar Transportation and Biofilm Formation in Streptococcus mutans
Gong, mSystems 2021 - “...SMU_1183 , SMU_1184c , and SMU_1185 ) and the trehalose-specific PTS system ( SMU_2037 and SMU_2038 ) ( Fig.8A ). The promoter regions ( P SMU_1185 and P SMU_2038 ) were used to search for a generally conserved motif for RcrR binding using MEME Suite 5.1.1...”
- “...PTS operons. The expression levels of SMU_1183 , SMU_1184c , SMU_1185 , SMU_2037 , and SMU_2038 in the rcrR -overexpressing strain were found to be upregulated 2- to 7-fold compared with the wild-type strain by quantitative reverse transcription-PCR (qRT-PCR) ( Fig.9B ). The predicted binding sequences...”
- Protein Interactomes of Streptococcus mutans YidC1 and YidC2 Membrane Protein Insertases Suggest SRP Pathway-Independent- and -Dependent Functions, Respectively
Lara, mSphere 2021 - “...component (SMU_2047) Conserved hypothetical protein, cyclic nucleotide-binding protein (SMU_1307c) Putative PTS system, trehalose-specific IIABC component (SMU_2038) Putative cell shape-determining protein MreC (SMU_20) Putative glycosyltransferase, RgpI (SMU_833) Conserved hypothetical protein (SMU_1477c) Putative undecaprenyl-phosphate-UDP- MurNAc-pentapeptide transferase (SMU_456) Putative cell division protein RodA (SMU_1279c) Integral membrane protein possibly involved...”
- Characterization of the Trehalose Utilization Operon in Streptococcus mutans Reveals that the TreR Transcriptional Regulator Is Involved in Stress Response Pathways and Toxin Production
Baker, Journal of bacteriology 2018 - “...SMU_2037 Putative trehalose-6-phosphate hydrolase TreA SMU_2038 Putative PTS, trehalose-specific IIABC component SMU_2040 Putative transcriptional regulator;...”
- RNA-Seq Reveals Enhanced Sugar Metabolism in Streptococcus mutans Co-cultured with Candida albicans within Mixed-Species Biofilms
He, Frontiers in microbiology 2017 - “...are channeled into the glycolytic pathway ( Chassy and Victoria Porter, 1979 ). Furthermore, pttB (SMU_2038), encoding a trehalose PTS permease, was also up-regulated in dual-species biofilms (Supplementary Table S1 ). Notably, the trehalose-PTS (i.e., EII Tre ), the primary transporter for trehalose ( Poy and...”
KSF55_11445 N-acetylglucosamine-specific PTS transporter subunit IIBC from Lactiplantibacillus pentosus
51% identity, 9% coverage
lp_0265 glucose PTS transporter subunit IIA from Lactiplantibacillus plantarum WCFS1
lp_0265 beta-glucosides PTS, EIIABC from Lactobacillus plantarum WCFS1
49% identity, 9% coverage
- Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study
Petrov, Genes 2021 - “...sensitive strain 8-1. We observed the strongest downregulation among the PTS transporters in two C-subunits: lp_0265 and lp_0264. They were 14.69- and 25.45-fold downregulated in 8-1 and 18.57- and 38.68-fold in Ym1, respectively. Based on a 73.2% match with the respective proteins in Listeria monocytogenes ,...”
- “...PTS N-acetylgalactosamine transporter IIC 12.45 2.88 lp_3009, pts23B PTS sugar transporter subunit IIB 2.19 3.02 lp_0265, pts5ABC PTS transporter subunit EIIC 18.57 14.69 lp_0264, pts4ABC PTS transporter subunit EIIC 38.68 25.45 lp_0170, dak3 PTS-dependent dihydroxyacetone kinase phosphotransferase subunit DhaM 4.86 164.91 * The difference in gene...”
- Transcriptomic Evidence of Molecular Mechanisms Underlying the Response of Lactobacillus Plantarum WCFS1 to Hydroxytyrosol
Reverón, Antioxidants (Basel, Switzerland) 2020 - “...transporters for these substances. These included the lp_3324 (glycine betaine/carnitine/choline transporter), lp_1722 (GABA transporter) and lp_0265 (trehalose PTS transport system) genes, which is coincident with the response of L. plantarum to p-coumaric acid [ 7 ], a phenolic compound that turns leaky the membrane of several...”
- Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts
Santamaría, Frontiers in microbiology 2019 - “...lp_0587 ), the sugar alcohol mannitol ( pts2CB or lp_0230 ), threalose ( pts5ABC or lp_0265 ), cellobiose ( pts6C or lp_0286 , pts20A or lp_2780 , pts20B or lp_2781 ) and fructose ( pts16ABC or lp_2097 ) were all induced in presence of OLE. In...”
- Transcriptional Reprogramming at Genome-Scale of Lactobacillus plantarum WCFS1 in Response to Olive Oil Challenge
Esteban-Torres, Frontiers in microbiology 2017 - “...3.04 lp_1449 Cell surface protein, CscB family 1.27 3.53 lp_2035 aroE 3-phosphoshikimate 1-carboxyvinyltransferase 1.34 3.71 lp_0265 pts5ABC PTS system trehalose-specific transporter subunit IIBC 2.16 3.23 lp_0007 gyrA DNA gyrase subunit A 0.69 2.93 Linear regression fit analysis was applied and correlation coefficient used to validate the...”
- “...included those within the trehalose operon [ lp_0262 (transcriptional repressor)], lp_0263 (trehalose-6-phosphate hydrolase), lp_0264 and lp_0265 (PTS system trehalose-specific transporter subunits IIBC), the GABA transporter ( lp_1722 ) and two aquaporins ( lp_0372 and lp_3436 ), which were all downregulated. This profile indicates that these compatible...”
- Involvement of the mannose phosphotransferase system of Lactobacillus plantarum WCFS1 in peroxide stress tolerance
Stevens, Applied and environmental microbiology 2010 - “...1.43E-02 2.01 Transport and binding proteins lp_0180 lp_0265 lp_0315 msmK1 pts5ABC potD Multiple sugar ABC transporter, ATP-binding protein PTS system,...”
SSU0217 sugar phosphotransferase system (PTS), IIABC component from Streptococcus suis P1/7
54% identity, 8% coverage
- FlpS, the FNR-Like Protein of Streptococcus suis Is an Essential, Oxygen-Sensing Activator of the Arginine Deiminase System
Willenborg, Pathogens (Basel, Switzerland) 2016 - “...In the exp growth phase the predicted PTS for fructose ( SSU0768 ), trehalose ( SSU0217 ) and glucose/mannose ( SSU1583 ), and in the stat growth phase the PTS for predicted transport of mannose ( SSU0199-201 ), galactose ( SSU0403-0406 ), and N-acetylgalactosamines ( SSU1055-1057...”
- “...genes found by our microarray analysis. SSU0357 and SSU1583 presumably encode for glucose-specific PTS whereas SSU0217 and SSU1309 encode for trehalose- and -glucoside PTS, respectively. Glucose and glucose-containing di- and oligosaccharides are the major energy sources of S. suis. Since expression of gapA and gpmA was...”
CHF17_RS01560 PTS system trehalose-specific EIIBC component from Streptococcus agalactiae
gbs0189 Unknown from Streptococcus agalactiae NEM316
52% identity, 9% coverage
EMQU_2186 beta-glucoside-specific PTS transporter subunit IIABC from Enterococcus mundtii QU 25
51% identity, 10% coverage
- Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose
Shiwa, PloS one 2020 - “...in S3 Table . Cellobiose induced genes for five putative PTS genes/clusters (EMQU_03070309, EMQU_08750877, EMQU_1036, EMQU_2186, and EMQU_2681). In addition, the genes for three glycoside hydrolase family 1 proteins (EMQU_0608, EMQU_1037, and EMQU_2185), which were annotated as 6-phospho-beta-glucosidase in KEGG, were significantly upregulated in the cellobiose...”
SMU_980, SMU_RS04505 beta-glucoside-specific PTS transporter subunit IIABC from Streptococcus mutans UA159
55% identity, 9% coverage
UC7_RS14195 beta-glucoside-specific PTS transporter subunit IIABC from Enterococcus caccae ATCC BAA-1240
49% identity, 10% coverage
SSU05_0398 Phosphotransferase system IIC component, glucose/maltose/N-acetylglucosamine-specific from Streptococcus suis 05ZYH33
54% identity, 9% coverage
- Multi-omics analysis reveals genes and metabolites involved in Streptococcus suis biofilm formation
Wang, BMC microbiology 2024 - “...formation [ 16 ]. The inactivation of this gene, impairs the S. suis biofilm formation. SSU05_0398 and SSU05_1401 both encode the glucose/maltose/N-acetylglucosamine-specific phosphotransferase system IIC component. The regulatory role of the phosphotransferase system (PTS) in the Streptococcus pyogenes biofilm formation has been demonstrated, and ptxA and...”
- The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis
Zhang, Frontiers in cellular and infection microbiology 2017 - “...vital in translation. Most genes in the glycolysis were significantly down-regulated, including ptsG (SSU05_0397), crr (SSU05_0398), pgk (SSU05_0157), adhA (SSU05_0279), and adhE (SSU05_0280) (Figure 4 ). Notably, expression levels of rpsN (SSU05_1535), rpsR (SSU05_1832), rpsP (SSU05_0796), rpsU (SSU05_1433), and glyS (SSU05_1764) translation-associated genes in the pathways...”
- “...of 36) involved in various PTSs were down-regulated, including manX/Y/Z (SSU05_17781780)encoding mannose transporter, ptsG/crr/bglF (SSU05_0397, SSU05_0398, and SSU05_1490) encoding maltose/glucose transporter, celC (SSU05_0709) encoding cellobiose transporter, and ulaA/ulaB (SSU05_2062, and SSU05_2063) encoding ascorbate transporter. Down-regulation of these pathways and related genes were potentially consistent with the...”
- In vitro transcriptome analysis of two Chinese isolates of Streptococcus suis serotype 2
Zhang, Genomics, proteomics & bioinformatics 2014 - “...PTS IIC component, glucose 05ZYH33 LacF SSU05_1038 1.11 PTS cellobiose-specific component IIA Pts / Crr SSU05_0398 1.56 PTS IIC component, glucose/maltose/ N -acetylglucosamine-specific AgaW SSU05_1218 2.59 PTS, mannose/fructose/ N -acetylgalactosamine-specific component IIC UlaA SSU05_0189 1.77 PTS system ascorbate-specific transporter subunit IIC ManY SSU05_0451 2.19 PTS, mannose/fructose/...”
- “...1.1.1.1 SSU98_0274 1.63 AdhA 1.1.1.1 SSU98_0275 1.92 Bifunctional acetaldehyde-CoA 3.2.1.86 SSU98_1500 6.14 Beta-glucosidase 05ZYH33 2.7.1.69 SSU05_0398 1.56 PTS IIC component, glucose/maltose/ N -acetylglucosamine-specific 3.2.1.86 SSU05_2079 1.69 Alpha-galactosidase/6-phospho-beta-glucosidase 1.1.1.1 SSU05_0279 2.29 Alcohol dehydrogenase 1.1.1.1 SSU05_0280 3.01 Bifunctional acetaldehyde-CoA/alcohol dehydrogenase 3.2.1.86 SSU05_1489 5.05 Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase Note: FC stands for...”
C289_1015 glucose-specific PTS transporter subunit IIBC from Anoxybacillus ayderensis
44% identity, 11% coverage
- A high molecular-mass Anoxybacillus sp. SK3-4 amylopullulanase: characterization and its relationship in carbohydrate utilization
Kahar, International journal of molecular sciences 2013 - “...C289_2139). Several putative sugar transporters (C289_0465, C289_0466, C289_0467, C289_0603, C289_0763, C289_0764, C289_0765, C289_0778, C289_0779, C289_0780, C289_1015, C289_1174, C289_1392, C289_1394, C289_1910, C289_1911, and C289_1912) were also found in the Blast2GO annotation. The draft genome was submitted to the National Center for Biotechnology Information (NCBI) Bioproject with accession...”
PTU3C_STACT / Q53922 PTS system glucoside-specific EIICBA component; EIICBA-Glc 2; EC 2.7.1.- from Staphylococcus carnosus (strain TM300) (see 2 papers)
TC 4.A.1.1.14 / Q53922 Glucose porter GlcB (IICBA). Glucose uptake is inhibited by methyl-α-D-glucoside, methyl-β-D-glucoside, p-nitrophenyl-α-D-glucoside, o-nitrophenyl-β-D-glucoside and salicin, but not by 2-deoxyglucose. Mannose and N-acetylglucosamine are not transported from Staphylococcus carnosus (strain TM300) (see 4 papers)
49% identity, 9% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in alpha- and beta-glucoside transport. Can also transport glucose, but not galactose, fructose, mannose, cellobiose, sucrose, maltose, lactose, melibiose and trehalose, as well as N- acetylglucosamine.
- substrates: glucose
Spaf_1559 PTS system trehalose-specific EIIBC component from Streptococcus parasanguinis FW213
52% identity, 9% coverage
- Complete genome and transcriptomes of Streptococcus parasanguinis FW213: phylogenic relations and potential virulence mechanisms
Geng, PloS one 2012 - “...Spaf_1806 Small-conductance mechanosensitive efflux channel 797 240 3.3 Spaf_1558 Putative trehalose-6P hydrolase 848 36 23.4 Spaf_1559 Trehalose-specific IIBC component 449 25 18.1 a , the PRKM was calculated as described in the materials and methods. b , the ratio of the expression levels in cells grown...”
- “...Additionally, a relatively high level of expression was seen in genes encoding the trehalose-specific EIIAB (Spaf_1559) and the trehalose-6 phosphate hydrolase (Spaf_1558) in the early growth phase. Trehalose is a compatible solute that accumulates intracellularly upon osmotic stress [58] and the expression of trehalose PTS in...”
SSU0357 glucose-specific phosphotransferase system (PTS), IIABC component from Streptococcus suis P1/7
SSU98_0384 Phosphotransferase system IIC component, glucose/maltose/N-acetylglucosamine-specific from Streptococcus suis 98HAH33
SSUSC84_0343 putative glucose-specific phosphotransferase system (PTS), IIABC component from Streptococcus suis SC84
54% identity, 7% coverage
- In vivo transcriptomes of Streptococcus suis reveal genes required for niche-specific adaptation and pathogenesis
Arenas, Virulence 2019 - “...genes with variable expression level at different infection sites and from different functional categories, i.e. SSU0357, SSU0899, and SSU1849. Figure S1 (supplemental material) shows gene expression profiles obtained by RT-qPCR and microarrays for each selected gene. Except for gene SSU0899, where transcription levels in blood and...”
- FlpS, the FNR-Like Protein of Streptococcus suis Is an Essential, Oxygen-Sensing Activator of the Arginine Deiminase System
Willenborg, Pathogens (Basel, Switzerland) 2016 - “...phenotypes corresponded to the affected expression of the respective genes found by our microarray analysis. SSU0357 and SSU1583 presumably encode for glucose-specific PTS whereas SSU0217 and SSU1309 encode for trehalose- and -glucoside PTS, respectively. Glucose and glucose-containing di- and oligosaccharides are the major energy sources of...”
- In vitro transcriptome analysis of two Chinese isolates of Streptococcus suis serotype 2
Zhang, Genomics, proteomics & bioinformatics 2014 - “...FC Protein PTS pathway 98HAH33 LacF SSU98_1051 3.05 PTS cellobiose-specific component IIA Pts / Crr SSU98_0384 1.92 PTS IIC component, glucose AgaW SSU98_1233 1.37 PTS, mannose UlaA SSU98_0191 1.38 Hypothetical protein ManY SSU98_0439 1.43 PTS, mannose LacE SSU98_0210 1.49 PTS cellobiose-specific component IIC CelB SSU98_2074 1.62...”
- “...component, glucose/maltose/ N -acetylglucosamine-specific Strain EC ID Gene ID FC Protein Glycolysis/gluconeogenesis pathway 98HAH33 2.7.1.69 SSU98_0384 1.92 PTS IIC component, glucose 3.2.1.86 SSU98_2083 1.02 Alpha-galactosidase 1.1.1.1 SSU98_0274 1.63 AdhA 1.1.1.1 SSU98_0275 1.92 Bifunctional acetaldehyde-CoA 3.2.1.86 SSU98_1500 6.14 Beta-glucosidase 05ZYH33 2.7.1.69 SSU05_0398 1.56 PTS IIC component, glucose/maltose/...”
- MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis
Tan, PloS one 2015 - “...transporter and MalT homolog (SP_0758) undertake this process [ 23 ]. while the MalT homolog (SSUSC84_0343) may be responsible for uptake of maltose and maltotrise in S . suis . Though the results of qRT-PCR, we found that MalT not only takes part in transport of...”
TC 4.A.1.1.11 / Q8DS05 The maltose/maltotriose porter, MalT (31% identical to 4.A.1.1.9) from Streptococcus mutans (see paper)
SMU_2047, SMU_RS09355 PTS transporter subunit IIBC from Streptococcus mutans UA159
54% identity, 7% coverage
- substrates: Maltose, Maltotriose
- S-glutathionylation proteome profiling reveals a crucial role of a thioredoxin-like protein in interspecies competition and cariogenecity of Streptococcus mutans
Li, PLoS pathogens 2020 - “...pyrG 438 CTP synthase Q8DTV1 pyrF 155 Orotidine 5'-phosphate decarboxylase Q8DTV0 pyrDB 24 Dihydroorotate dehydrogenase Q8DS05 Glycolysis/Gluconeogenesis ptsG 498 Putative PTS system, glucose-specific IIABC component Q8DV96 Peptidoglycan biosynthesis murF 449 UDP-N-acetylmuramoyl-tripeptideD-alanyl-D-alanine ligase Q8DST2 murE 99 UDP-N-acetylmuramoyl-L-alanyl-D-glutamateL-lysine ligase Q8DVE3 murD 260 UDP-N-acetylmuramoylalanineD-glutamate ligase S-glutathionylation of proteins is...”
- Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome of Streptococcus mutans
Qin, Microbiology spectrum 2024 - “...6 BMP family ABC transporter substrate-binding protein SMU_RS05220 5 Phosphate ABC transporter ATP-binding protein, PstB1 SMU_RS09355 3.7 PTS transporter subunit EIIC, PtsG SMU_RS07760 3.7 Trk system potassium transporter, TrkA SMU_RS05155 3.7 ABC transporter ATP-binding protein SMU_RS08905 2.8 PTS sugar transporter subunit IIB SMU_RS01295 2 Peptide ABC...”
- Identification of the antibacterial action mechanism of diterpenoids through transcriptome profiling
Moon, Frontiers in microbiology 2022 - “...metabolism. Locus ID Annotation Gene Gene expression (log 2 FC) * AA CA KA DM SMU_RS09355 PTS transporter subunit EIIC ptsG 1.39 3.48 2.10 2.37 SMU_RS08435 PTS beta-glucoside transporter subunit IIBCA scrA 0.68 3.83 2.06 2.13 SMU_RS04505 PTS transporter subunit EIIC bglP 0.62 4.11 2.20 2.14...”
- Protein Interactomes of Streptococcus mutans YidC1 and YidC2 Membrane Protein Insertases Suggest SRP Pathway-Independent- and -Dependent Functions, Respectively
Lara, mSphere 2021 - “...ATP-binding protein ComA (SMU_286) Hypothetical protein APQ13_07375 (ParE_toxin) (SMU_40) Putative PTS system, glucose-specific IIABC component (SMU_2047) Conserved hypothetical protein, protease (SMU_235) RgpH (SMU_832) Putative cell division protein FtsH (SMU_15) Putative amino acid ABC transporter, periplasmic amino acid-binding protein (SMU_933) Putative PTS system, glucose-specific IIABC component (SMU_2047)...”
- “...kinase (SMU_486) Histidine kinase (SMU_486) Cell division protein FtsH (SMU_15) Putative PTS system, glucose-specific IIABC (SMU_2047) Putative amino acid ABC transporter, permease protein (SMU_1216c) Putative ABC transporter, substrate-binding protein (SMU_651c) Dextranase (SMU_2042) Hypothetical protein (SMU_791c) Conserved hypothetical protein (SMU_485) Potassium transporter peripheral membrane protein (SMU_1708) EamA...”
- Small noncoding RNA sRNA0426 is involved in regulating biofilm formation in Streptococcus mutans
Yin, MicrobiologyOpen 2020 - “...38 827,341 827,378 + SMU_875c SMU_876 //+// IGR GTATCGCAAACGTTTGCAAAACTGTTCCTTTCTAATAA >sRNA0679 53 1,922,203 1,922,255 + SMU_2046c SMU_2047 //+// AM AATCTCAAGCAAAGACTTTTTAGATTCTAGCCTACTCCTTTTTAATCTTTTTA Note The data are extracted from Zhu et al. ( 2018 ). AM indicates that the sRNA is located on the antisense strand to mRNA; IGR indicates...”
- A five-species transcriptome array for oral mixed-biofilm studies
Redanz, PloS one 2011 - “...Environmental Information Processing SMU_1934c putative cobalt ABC transporter, ATP-binding protein 2.07 down Metabolism, EnvironmentalInformation Processing SMU_2047 ptsG - putative PTS system, glucose-specific IIABC component 2.13 up SMU_311 PTS system, sorbitol (glucitol) phosphotransferase enzyme IIC2 3.41 up SMU_312 PTS system, sorbitol phosphotransferase enzyme IIBC 2.93 up SMU_313...”
- “...LacB 2.09 (0.52) 2.03 (p=0.15) SMU_1954 groEL ; putative chaperonin GroEL 2.15 (0.83) 2.33 (p=0.075) SMU_2047 ptsG ; putative PTS system, glucose-specific IIABC component 3.88 (2.79) 2.13 (p<0.05) downregulated genes SMU_262 otcA ; putative ornithine carbamoyltransferase 2.39 (1.55) 2.07 (p<0.05) SMU_498 comF ; putative late competence...”
SMUGS5_09220 PTS transporter subunit IIBC from Streptococcus mutans GS-5
54% identity, 7% coverage
- D‑Tagatose inhibits the growth and biofilm formation of Streptococcus mutans
Hasibul, Molecular medicine reports 2018 - “...EII-fru/man-F aagactctctttacggtgggttc 111 EII-fru/man-R agtgaaaccaacaaagaatggaa SMUGS5_08440 Glucose/Mannose-specific PTS subunit IIAB EII-glu/man-F ctaaagctgaccgtatcattgttg 111 EII-glu/man-R attggtacaacattggccttgaca SMUGS5_09220 Maltose/Glucose-specific PTS subunit IIABC EII-mal/glu-F acacaatatctttggcggtaaga 105 EII-mal/glu-R cgaaaccagagataataccaacg SMUGS5_03875 Fructose-specific PTS subunit IIABC EII-fru-F tgatacttttaaggctgggatca 116 EII-fru-R aaaagaaccgttgcttcctttac SMUGS5_08275 Sucrose-specific PTS subunit IIABC EII-sucF ggtgtctttggtgctctgtattc 113 EII-suc-R gtcaccatgaccagtaccatttt SMUGS5_R09892...”
BFP66_RS08570 beta-glucoside-specific PTS transporter subunit IIABC from Streptococcus suis
55% identity, 9% coverage
- A novel aquaporin Aagp contributes to Streptococcus suis H2O2 efflux and virulence
Zhu, Virulence 2023 - “...IICB; BFP66_RS08510 10.66 PTS cellobiose transporter subunit IIC; BFP66_RS08530 53.00 PTS cellbiose transporter subunit IIC; BFP66_RS08570 5.96 PTS beta-glucoside transporter subunit EIIBCA; BFP66_RS09840 2.24 PTS sugar transporter subunit IIC; BFP66_RS09850 4.61 PTS lactose/cellobiose transporter subunit IIA; BFP66_RS09855 3.09 PTS sugar transporter subunit IIB; BFP66_RS05750 3.95 aquaporin...”
BCAL_RS06200 PTS system trehalose-specific EIIBC component from Bifidobacterium callitrichos DSM 23973
52% identity, 8% coverage
- The comparative genomics of Bifidobacterium callitrichos reflects dietary carbohydrate utilization within the common marmoset gut
Albert, Microbial genomics 2018 - “...BCAL_RS02285 Starch and sucrose metabolism ,-Phosphotrehalase 3.2.1.93 COO72_RS08005 CPA40_RS06370 BCAL_RS06195 Protein-N-phosphohistidinetrehalose phosphotransferase 2.7.1.201 COO72_RS08010 CPA40_RS06365 BCAL_RS06200 -Fructofuranosidase 3.2.1.26 COO72_RS02000 CPA40_RS06035 BCAL_RS10725 -Glucosidase 3.2.1.20 COO72_RS09510 CPA40_RS01270 BCAL_RS0830 Amylosucrase 2.4.1.4 COO72_RS05900 CPA40_RS04855 BCAL_RS04290 Sucrose phosphorylase 2.4.1.7 COO72_RS10000 CPA40_RS07310 BCAL_RS06670 UTPglucose-1-phosphate uridylyltransferase 2.7.7.9 COO72_RS10475 CPA40_RS09980 BCAL_RS08755 -Amylase 3.2.1.1 COO72_RS11340...”
CD0388 PTS system, beta-glucoside-specific IIabc component from Clostridium difficile 630
51% identity, 9% coverage
CD3137 PTS system, IIabc component from Clostridium difficile 630
51% identity, 10% coverage
- Pleiotropic role of the RNA chaperone protein Hfq in the human pathogen Clostridium difficile
Boudry, Journal of bacteriology 2014 - “...CD3030), one mannose (CD3013-CD3015), and one betaglucoside-specific (CD3137) transporter were downregulated in the Hfq-depleted strain while genes encoding a...”
- Temporal differential proteomes of Clostridium difficile in the pig ileal-ligated loop model
Janvilisri, PloS one 2012 - “...proteins involved in carbohydrate utilization through phosphotransferase systems (PTS) including PtsG (CD2667), MalX (CD3030), and CD3137 were down-regulated, others including CD0287, GatC (CD2325), PtsH (CD2756), CD3068, and CD3069 were found to be up-regulated. These results suggest that the changes in metabolic states of C. difficile within...”
SSA_0379 PTS system, beta-glucoside-specific EII component, putative from Streptococcus sanguinis SK36
48% identity, 10% coverage
SXYL_00060 PTS cellobiose/arbutin/salicin transporter subunit IIBC from Staphylococcus xylosus
52% identity, 9% coverage
- Insight into the Genome of Staphylococcus xylosus, a Ubiquitous Species Well Adapted to Meat Products
Leroy, Microorganisms 2017 - “...SXYL_00886 scrR Repressor SXYL_00577 Lactate permease SXYL_00887-88 scrBK Hydrolase, Fructokinase SXYL_00170 lactate-quinone oxidoreductase others Lactose SXYL_00060, SXYL_00626 Arbutin, EIIBC SXYL_00082 lacR Transcription activator SXYL_00528 Beta-glucoside, EIIABC SXYL_00083-84 lacPH Permease, Beta-galactosidase SXYL_00257-60 Cellobiose, EIIC, EIIBA SXYL_00671 galR Transcriptional regulator SXYL_02148-50 fruAK Fructose, EIIABC, catabolism, repression SXYL_00672-74 galKET...”
lp_2531 N-acetylglucosamine and glucose PTS, EIICBA from Lactobacillus plantarum WCFS1
lp_2531 N-acetylglucosamine-specific PTS transporter subunit IIBC from Lactiplantibacillus plantarum WCFS1
49% identity, 9% coverage
- Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study
Petrov, Genes 2021 - “...transporters, half of them concerned with the membrane-bound subunit C. From those upregulated in 8-1, lp_2531 (FC +3.22) appears to be specific for N-acetylglucosamine and may play a role in the recycling of peptidoglycan. All 19 genes are downregulated in 8-1, including some encoding PTS transporters...”
- “...sorbitol transporter subunit IIC +2.29 NC lp_0286, pts6C PTS cellobiose transporter subunit IIC +2.55 NC lp_2531, pts18CBA PTS transporter subunit EIIC NC +3.22 lp_0886, pts11BC PTS transporter subunit EIIC NC 3.91 lp_3240, pts28ABC PTS transporter subunit EIIC NC 2.19 lp_0436, pts7C PTS sugar transporter subunit IIC...”
- Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts
Santamaría, Frontiers in microbiology 2019 - “...lp_3513 ) and PTS systems for the transport of N -acetyl-glucosamine (GlcNAc) ( pts18CBA or lp_2531 ), N -acetyl-galactosamine (GalNAc) ( pts19B or lp_2650 ) and GlcNAc/GalNAc ( pts19A or lp_2647 ) were downregulated. Oleuropein Affects Biological Functions Involved in Cell Proliferation The DAVID analysis also...”
- Identification of genetic loci in Lactobacillus plantarum that modulate the immune response of dendritic cells using comparative genome hybridization
Meijerink, PloS one 2010 - “...compared to the wild type. In the mutant, the largest decrease in expression was for lp_2531 which is predicted to be a PTS transporter for N-acetylglucosamine and glucose. RT-PCR results Quantitative reverse transcription-PCR (RT-PCR) was performed to validate the results of the transcriptome analysis for mntH2...”
- Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays
Saulnier, Applied and environmental microbiology 2007 - “...lp_1468 lp_1469 lp_1471 lp_1472 lp_1473 lp_1686 lp_1921 lp_2531 lp_2649 lp_2650 lp_2710 lp_2776 lp_2789 lp_2982 lp_2984 lp_3491 lp_3533 lp_3543 lp_3665 Mannitol...”
lmo0738 similar to phosphotransferase system (PTS) beta-glucoside-specific enzyme IIABC component from Listeria monocytogenes EGD-e
LMON_0743 beta-glucoside-specific PTS transporter subunit IIABC from Listeria monocytogenes EGD
56% identity, 8% coverage
- RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection
Stamm, mBio 2024 - “...with 1 10 5 CFU of the indicated strains ( n = 3 for alsR lmo0738 and n = 5 for all other strains) and CFU were enumerated from livers at 72 hpi. Mean WT and alsR CFU were calculated from at least four independent experiments....”
- “...( Fig. 4G ; Fig. S4J). In contrast, alsR lmo0736 , alsR lmo0737 , alsR lmo0738, and alsR lmo0739 CFU were similar to alsR CFU ( Fig. 4G ; Fig. S4K through N). In all, no single ALO gene deletion rescued the growth of alsR to...”
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...3.13 Yes/down lmo0632 PTS fructose transporter subunit IIC lmo0633 lmo0633 PTS fructose transporter subunit IIB lmo0738 lmo0738 PTS beta-glucoside transporter subunit IIABC lmo0781 1.33 Yes/down lmo0781 PTS mannose transporter subunit IID lmo0782 3.54 Yes/down lmo0782 PTS mannose transporter subunit IIC lmo0783 1.43 Yes/down lmo0783 PTS mannose...”
- New Insights into the Lactic Acid Resistance Determinants of Listeria monocytogenes Based on Transposon Sequencing and Transcriptome Sequencing Analyses
Liu, Microbiology spectrum 2023 - “...sorbitol transporter subunit IIBC 0.1233 5.02E-05 lmo0544 lmo0544 PTS sorbitol transporter subunit IIC 0.0485 9.23E-05 lmo0738 lmo0738 PTS beta-glucoside transporter subunit IIABC 0.0009 0.00E+00 lmo0781 lmo0781 PTS mannose transporter subunit IID 0.4964 3.58E-05 lmo0874 lmo0874 PTS sugar transporter subunit IIA 0.1111 3.00E-02 lmo0875 lmo0875 PTS beta-glucoside...”
- Horizontal Gene Transfer and Loss of Serotype-Specific Genes in Listeria monocytogenes Can Lead to Incorrect Serotype Designations with a Commonly-Employed Molecular Serotyping Scheme
Brown, Microbiology spectrum 2023 - “...Interestingly, the GC content of the cassette was heterogeneous, with some genes (e.g., lmo0737 , lmo0738 ) having GC values similar to the L. monocytogenes chromosomal average of approximately 38% ( 33 ), while the GC content of others (e.g., lmo0734 , lmo0735 ) was much...”
- YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes
Cheng, Gut microbes 2021 - “...IIB Yes lmo0633 PTS fructose-specific enzyme IIB Yes lmo2799 PTS mannitol-specific enzyme IIBC 10.27 Yes lmo0738 PTS beta-glucoside-specific enzyme IIABC 9.85 Yes lmo2666 PTS galacticol-specific enzyme IIB 4.79 Yes lmo2650 PTS L-ascorbate-specific enzyme IIB 4.02 Yes lmo1255 PTS trehalose-specific enzyme IIBC 2.93 Yes lmo2002 PTS mannose-specific...”
- Identification and Characterization of als Genes Involved in D-Allose Metabolism in Lineage II Strain of Listeria monocytogenes
Zhang, Frontiers in microbiology 2018 - “...lmo0737 might be the equivalent to alsK , being responsible for phosphorylation for D-allose. Gene lmo0738 is annotated as encoding a PTS beta-glucoside transporter subunit IIABC in EGD-e, which has the same function as the products of alsA, B , and C , namely transferring D-allose...”
- “...reported to be dispensable in D-allose metabolism in other bacteria. Nonetheless, absence of lmo0737 and lmo0738 decreased the growth rate in D-allose MWB medium. The requirement for lmo0734 to lmo0736 and the dispensability of lmo0738 to lmo0739 suggested that lmo0734 to lmo0736 are irreplaceable, while other...”
- A Comprehensive Evaluation of the Genetic Relatedness of Listeria monocytogenes Serotype 4b Variant Strains
Burall, Frontiers in public health 2017 - “...regulator (lmo0734), a putative ribulose-5-phosphate 3-epimerase (lmo0735), a putative ribose-5-phosphate isomerase (lmo0736), phosphotransferase system IIABC (lmo0738), and a glucosidase (lmo0739). The protein encoded by lmo0737 has no annotated function. Prior work generating a deletion of this region in EGD-e, a laboratory-adapted 1/2a strain, found no corresponding...”
- “...4bV had serine in all but one strain, instead of a proline, at site 237. Lmo0738 had four amino acid substitutions: alanine to valine at amino acid 40, valine to isoleucine at 470, asparagine to serine at 483, and leucine to isoleucine at position 538. Lmo0738...”
- More
- Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Hingston, PloS one 2017 - “...-1.36 -1.52 -0.35 10.41 LMON_0742 Hypothetical protein + lmo0737 2 0.04 -0.98 -1.86 1.08 10.49 LMON_0743 PTS system, D-allose-specific + lmo0738 2 -0.61 -0.29 -0.59 1.52 9.56 LMON_0744 6-phospho-beta-glucosidase + lmo0739 2 -0.85 -0.15 -0.41 0.86 8.78 LMON_2673 PTS system, lactose/cellobiose specific IIB subunit lmo2650 2...”
str1734 sucrose-specific PTS permease, enzyme II from Streptococcus thermophilus CNRZ1066
52% identity, 9% coverage
SGO_0505 PTS system, IIBC component from Streptococcus gordonii str. Challis substr. CH1
54% identity, 7% coverage
STER_1710, STER_RS08355 sucrose-specific PTS transporter subunit IIBC from Streptococcus thermophilus LMD-9
52% identity, 9% coverage
- Identification of Hanks-Type Kinase PknB-Specific Targets in the Streptococcus thermophilus Phosphoproteome
Henry, Frontiers in microbiology 2019 - “...Pyruvate formate lyase YP_820965.1 WP_011226473.1 STER_1622 STER_RS07960 ScrA Sucrose PTS transporter subunit EIIBCA YP_821049.1 WP_011681617.1 STER_1710 STER_RS08355 GalU UDP-glucose-pyrophosphorylase YP_821134.1 WP_002953557.1 STER_1810 STER_RS08845 Cell cycle control GpsB Cell division protein YP_819787.1 WP_011680702.1 STER_0280 STER_RS01360 PabC Transporter/YceG motif involved in septum cleavage YP_819793.1 WP_011680707.1 STER_0288 STER_RS01395 FtsW...”
- “...formate lyase YP_820965.1 WP_011226473.1 STER_1622 STER_RS07960 ScrA Sucrose PTS transporter subunit EIIBCA YP_821049.1 WP_011681617.1 STER_1710 STER_RS08355 GalU UDP-glucose-pyrophosphorylase YP_821134.1 WP_002953557.1 STER_1810 STER_RS08845 Cell cycle control GpsB Cell division protein YP_819787.1 WP_011680702.1 STER_0280 STER_RS01360 PabC Transporter/YceG motif involved in septum cleavage YP_819793.1 WP_011680707.1 STER_0288 STER_RS01395 FtsW FtsW/RodA/SpoVE...”
- Adaptor protein MecA is a negative regulator of the expression of late competence genes in Streptococcus thermophilus
Boutry, Journal of bacteriology 2012 - “...STER_1602 STER_1639 STER_1683 STER_1684 STER_1709 STER_1710 ldh ABC-type Fe3-siderophore transport system, permease component L-Lactate dehydrogenase...”
stu1734 sucrose PTS component II from Streptococcus thermophilus LMG 18311
52% identity, 9% coverage
T303_09505 sucrose-specific PTS transporter subunit IIBC from Streptococcus thermophilus ASCC 1275
52% identity, 9% coverage
SGO_RS01385 beta-glucoside-specific PTS transporter subunit IIABC from Streptococcus gordonii str. Challis substr. CH1
SGO_0281 PTS system, beta-glucoside-specific IIABC component from Streptococcus gordonii str. Challis substr. CH1
48% identity, 10% coverage
- Interactions Between Streptococcus gordonii and Fusobacterium nucleatum Altered Bacterial Transcriptional Profiling and Attenuated the Immune Responses of Macrophages
Liu, Frontiers in cellular and infection microbiology 2021 - “...fusions (red), cooccurrence (blue), and coexpression (black). The genes in the central cluster include SGO_0281 (SGO_RS01385, PTS glucose transporter subunit IIA, up-regulated), SGO_1857 (SGO_RS09095, PTS beta-glucoside transporter subunit IIBCA, down-regulated), and SGO_1858 (SGO_RS09100, sucrose-6-phosphate hydrolase, down-regulated). These genes encode proteins that participate in the carbohydrate transport...”
- Interactions Between Streptococcus gordonii and Fusobacterium nucleatum Altered Bacterial Transcriptional Profiling and Attenuated the Immune Responses of Macrophages
Liu, Frontiers in cellular and infection microbiology 2021 - “...gene fusions (red), cooccurrence (blue), and coexpression (black). The genes in the central cluster include SGO_0281 (SGO_RS01385, PTS glucose transporter subunit IIA, up-regulated), SGO_1857 (SGO_RS09095, PTS beta-glucoside transporter subunit IIBCA, down-regulated), and SGO_1858 (SGO_RS09100, sucrose-6-phosphate hydrolase, down-regulated). These genes encode proteins that participate in the carbohydrate...”
CA_C0423 sucrose-specific PTS transporter subunit IIBC from Clostridium acetobutylicum ATCC 824
42% identity, 11% coverage
PTSA_BACSU / P50829 Phosphotransferase enzyme IIA component PtsA; PTS system EIIA component from Bacillus subtilis (strain 168) (see paper)
49% identity, 34% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (Probable). Plays an important role in the phosphorylation of EIIA- deficient PTS transporters (PubMed:30038046). The EIIA domain can transfer a phosphoryl group to EIIA-deficient PTS transporters, enabling growth with maltose, N-acetylglucosamine, sucrose or trehalose as the sole carbon source (PubMed:30038046).
UH47_06750 glucose-specific PTS transporter subunit IIBC from Staphylococcus pseudintermedius
50% identity, 10% coverage
- Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius
Magnowska, Scientific reports 2019 - “...point T T P T T P 30 90 90 30 90 90 Central metabolism UH47_06750 PTS system glucose-specific transporter subunit 1 glycolysis UH47_06270 PTS system glucose-specific transporter subunit 1.2 glycolysis UH47_12920 glyceraldehyde-3-phosphate dehydrogenase 2.1 1.5 glycolysis/gluconeogenesis UH47_11065 phosphoglyceromutase gpmI 1.3 glycolysis/gluconeogenesis UH47_11070 triosephosphate isomerase tpi...”
- “...consistent alterations were observed for carbohydrate uptake systems. Glucose uptake by the glucose-specific transporter (UH47_06270, UH47_06750) was downregulated, whereas expression of numerous alternative carbohydrate carbon source uptake and activation proteins were triggered, i.e. trehalose (permease, UH47_02750; 6-phosphate hydrolase, treC ), glucoside (specific transporter, UH47_02965), glucarate (transporter,...”
BJK46_009280 glucose-specific PTS transporter subunit IIBC from Staphylococcus pseudintermedius
50% identity, 10% coverage
- Erythritol alters gene transcriptome signatures, cell growth, and biofilm formation in Staphylococcus pseudintermedius
Fujii, BMC veterinary research 2023 - “...to two biological functional groups were present. Three genes (Up-Regulated Genes group 1, URGs1 ), BJK46_009280 ( ptsG ), BJK46_000300 ( ppdK ), and BJK46_000295 ( ppdkR ), were predicted to encode proteins related to the PTS. The ptsG gene encodes the glucose specific EIICBA component...”
- “...BJK46_008085 hypothetical protein 4.73 0 BJK46_005200 M50 family peptidase 0.67 M50 family metallopeptidase 4.63 2.45E-302 BJK46_009280 PTS glucose transporter subunit IICBA ptsG 0.72 PTS system glucose-specific EIICBA component 0.82 GO:1,904,659 glucose transmembrane transport 0.72 GO:0009401 phosphoenolpyruvate-dependent sugar phosphotransferase system 0.59 GO:0016310 phosphorylation 4.62 2.79E-249 BJK46_006810 LytR...”
Cbei_0751 PTS system, glucose subfamily, IIA subunit from Clostridium beijerincki NCIMB 8052
43% identity, 11% coverage
SP_0577 PTS system, beta-glucosides-specific IIABC components from Streptococcus pneumoniae TIGR4
54% identity, 10% coverage
SPD_0502 PTS system, beta-glucosides-specific IIABC components from Streptococcus pneumoniae D39
spr0505 Phosphotransferase system sugar-specific EII component from Streptococcus pneumoniae R6
54% identity, 10% coverage
- The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance
De, PLoS pathogens 2024 - “...13.7 2.71 SPD_0460 dnaK protein chaperone 3.97 2.01 SPD_0501 licT -glucoside operon antiterminator 2.91 4.77 SPD_0502 bglF -glucoside PTS transporter subunit 3.07 5.65 SPD_0503 bglA-2 6-phospho--glucosidase 2.57 4.79 SPD_0615 glnH3 degenerate glutamine ABC transporter subunit 11.6 4.05 SPD_0616 glnQ3 glutamine ABC transporter subunit 8.90 3.07 SPD_0617...”
- “...3.50 3.52 SPD_0474 blpZ immunity protein 2.40 2.05 SPD_0501 licT -glucoside operon antiterminator 2.91 5.26 SPD_0502 bglF -glucoside PTS transporter subunit 3.07 4.65 SPD_0503 bglA-2 6-phospho--glucosidase 2.57 3.75 SPD_0537 putative Zn-dependent protease 2.07 2.21 SPD_0615 glnH3 degenerate glutamine ABC transporter subunit 11.6 18.0 SPD_0616 glnQ3 glutamine...”
- Inactivation of Transcriptional Regulator FabT Influences Colony Phase Variation of Streptococcus pneumoniae
Zhang, mBio 2021 - “...SPD_0453 hsdS Restriction endonuclease subunit S 2.93 0.000 SPD_0501 licT Transcription antiterminator Lict 1.95 0.000 SPD_0502 PTS glucose transporter subunit IIA 1.67 0.000 SPD_0503 bglA-2 6-Phospho-beta-glucosidase 1.69 0.000 SPD_0646 DegV family protein 1.49 0.000 SPD_0670 VanZ family protein 0.62 0.042 SPD_0671 ABC transporter ATP-binding protein 0.80...”
- Pivotal Roles for Ribonucleases in Streptococcus pneumoniae Pathogenesis
Sinha, mBio 2021 - “...dnaJ Chaperone protein DnaJ 2.96 6.49E09 SPD_0501 bglG Beta-glucoside bgl operon antiterminator BglG 13.4 2.46E20 SPD_0502 bglF Beta-glucoside-specific IIBCA components 11.0 2.14E38 SPD_0503 bglA-2 6-Phospho-beta-glucosidase 7.51 4.26E39 SPD_0522 vex2 ABC transporter ATP-binding protein Vex2 1.82 1.82E03 SPD_0523 vex3 Peptide ABC transporter membrane-spanning permease Vex3 1.85 9.48E04...”
- Interplay Between Capsule Expression and Uracil Metabolism in Streptococcus pneumoniae D39
Carvalho, Frontiers in microbiology 2018 - “...among the differentially expressed genes (Table S3 ). Noteworthy, eight PTS uptake systems ( spd_0066-9, spd_0502, spd_0559-61, spd_0661, spd_1047-8, spd_1057, spd_1959 , and spd_1992-89 ), and the enzymes involved in the metabolism of the PTS-incoming sugars ( spd_0063, spd_0065, spd_0070-1, spd_0503, spd_0562, spd_1046, spd_1958-7 , and...”
- Pneumococcal 6-Phospho-β-Glucosidase (BglA3) Is Involved in Virulence and Nutrient Metabolism
Terra, Infection and immunity 2016 - “...the strains mutated in 6-phospho--glucosidase genes, in SPD_0277, SPD_0502, or both, could grow as well as the wild-type strain on cellobiose, showing that it...”
- Ascorbic acid-dependent gene expression in Streptococcus pneumoniae and the activator function of the transcriptional regulator UlaR2
Afzal, Frontiers in microbiology 2015 - “...spd_0169 Riboflavin biosynthesis protein, RibD 2.1 spd_0262 PTS system, mannose/fructose/sorbose family protein, IID component 2.2 spd_0502 PTS system, beta-glucosides-specific IIABC components 2.3 spd_0503 6-Phospho-beta-glucosidase, BglA-2 2.9 spd_0773 PTS system, fructose specific IIABC components 2.4 spd_0888 Adhesion lipoprotein, AdcAII/LmB 3.2 spd_0889 Pneumococcal histidine triad protein D, PhtD...”
- Transcriptional and metabolic effects of glucose on Streptococcus pneumoniae sugar metabolism
Paixão, Frontiers in microbiology 2015 - “...phosphotransferase system IIC component 1.91 2.24 1.61 SPD_0360 mtlA PTS system, mannitol-specific IIBC components 0.62 SPD_0502 PTS system, beta-glucosides-specific IIABC components 0.65 0.47 0.73 SPD_0559 PTS system IIA component, putative 1.26 SPD_0560 PTS system, IIB component, putative 1.21 SPD_0561 PTS system, IIC component, putative 1.55 SPD_0661...”
- A non-linear deterministic model for regulation of diauxic lag on cellobiose by the pneumococcal multidomain transcriptional regulator CelR
Boianelli, PloS one 2012 - “...lag phase. Analysis of mutants for other PTS systems indicates that a second beta-glucoside PTS (spr0505), not able to support growth on cellobiose, is responsible for the lag during diauxic growth. A mathematical model of the process is devised together with a nonlinear identification procedure which...”
- “...the CelR EIIA domain is deduced from our growth phenotypes. The putative interaction of the spr0505 EIIBA domains with the PRD domains of CelR is shown as dashed line. In this work we address the contribution of phosphorylation of the phosphorylable cysteine in the EIIB domain...”
- A functional genomics approach to establish the complement of carbohydrate transporters in Streptococcus pneumoniae
Bidossi, PloS one 2012 - “...spr0356-8 PTS-Fru mtlAF indel mannitol mannitol [54] SP0474-6-8 SPG00432-4 spr0423-5 PTS-Lac indel mannose SP0577 SPG00525 spr0505 PTS-Glc bglP indel 1-O-Methyl--glucoside beta-glucosides [55] SP0645-6-7 SPG00585-6-7 spr0562-3-4 PTS-Gat gatABC conserved galactose galactose, lactose, galactitol [56] SP0758 SPG00690 spr0668 PTS-Glc malT conserved maltose, maltotriose, maltodextrin and glycogen maltose [58]...”
- Influences of capsule on cell shape and chain formation of wild-type and pcsB mutants of serotype 2 Streptococcus pneumoniae
Barendt, Journal of bacteriology 2009 - “...relative expression spr0088 spr0128 spr0446 spr0504 spr0505 spr0506 spr0778 spr0779 spr0780 Increased relative expression spr0096 spr0307 spr0445 spr0565...”
HSISS4_01641 sucrose-specific PTS transporter subunit IIBC from Streptococcus salivarius
50% identity, 9% coverage
TC 4.A.1.2.6 / Q9KJ80 β-glucoside (Aesculin/arbutin) porter, BglP from Streptococcus mutans (see paper)
53% identity, 9% coverage
BH0844 PTS system, glucose-specific enzyme II, A component from Bacillus halodurans C-125
44% identity, 11% coverage
PTG3C_STACT / Q57071 PTS system glucose-specific EIICBA component; EIICBA-Glc; EII-Glc; EIICBA-Glc 1; EC 2.7.1.199 from Staphylococcus carnosus (strain TM300) (see 2 papers)
TC 4.A.1.1.13 / Q57071 Glucose porter, GlcA (IICBA). Glucose uptake is inhibited by 2-deoxyglucose and methyl-β-D-glucoside from Staphylococcus carnosus (strain TM300) (see 4 papers)
46% identity, 10% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport. Cannot transport galactose, fructose, mannose, cellobiose, sucrose, maltose, lactose, melibiose and trehalose, as well as N-acetylglucosamine.
catalytic activity: N(pros)-phospho-L-histidyl-[protein] + D-glucose(out) = D- glucose 6-phosphate(in) + L-histidyl-[protein] (RHEA:33367) - substrates: glucose
LMOf2365_0030 PTS system, beta-glucoside-specific, IIABC component from Listeria monocytogenes str. 4b F2365
lmo0027 similar to PTS system, beta-glucosides specific enzyme IIABC from Listeria monocytogenes EGD-e
LMOf6854_0030 PTS system, beta-glucoside-specific, IIABC component from Listeria monocytogenes str. 1/2a F6854
LMRG_02456 PTS system, beta-glucoside-specific, IIABC component from Listeria monocytogenes 10403S
LM6179_0306, LMRG_RS00135 beta-glucoside-specific PTS transporter subunit IIABC from Listeria monocytogenes 10403S
46% identity, 10% coverage
- Transcriptomics of Listeria monocytogenes Treated With Olive Leaf Extract
Liu, Frontiers in microbiology 2021 - “...period (Log 2 FC=5.71, 1.36, 1.47, and 1.13, respectively). At 24h post-treatment, 9 PTS-related genes (LMOf2365_0030, LMOf2365_0113, LMOf2365_0572, LMOf2365_0922, LMOf2365_1272, LMOf2365_2128, LMOf2365_2129, LMOf2365_2130, and LMOf2365_2292) were found to be differentially regulated; 7 of which were found to be downregulated in the presence of OLE while LMOf2365_2129...”
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...lmo0023 PTS fructose transporter subunit IIC lmo0024 8.35 Yes/down lmo0024 PTS mannose transporter subunit IID lmo0027 6.99 Yes/down lmo0027 PTS beta-glucoside transporter subunit IIABC lmo0034 2.34 Yes/down lmo0034 PTS cellbiose transporter subunit IIC lmo0096 2.26 Yes/down lmo0096 PTS mannose transporter subunit IIAB lmo0097 3.61 Yes/down lmo0097...”
- New Insights into the Lactic Acid Resistance Determinants of Listeria monocytogenes Based on Transposon Sequencing and Transcriptome Sequencing Analyses
Liu, Microbiology spectrum 2023 - “...fructose transporter subunit IIA 0.2244 2.05E-07 lmo0023 lmo0023 PTS fructose transporter subunit IIC 0.0331 6.73E-13 lmo0027 lmo0027 PTS beta-glucoside transporter subunit IIABC 0.0224 9.12E-287 lmo0034 lmo0034 PTS cellobiose transporter subunit IIC 0.2085 2.15E-05 lmo0298 lmo0298 PTS beta-glucoside transporter subunit IIC 0.0113 2.36E-40 lmo0299 lmo0299 PTS beta-glucoside...”
- Roles of Alternative Sigma Factors in Invasion and Growth Characteristics of Listeria monocytogenes 10403S Into Human Epithelial Colorectal Adenocarcinoma Caco-2 Cell
Rukit, Frontiers in microbiology 2022 - “...L. monocytogenes during infection. Among intracellularly upregulated genes in sigL strain, glucose utilization genes (e.g., lmo0027 encoding a PTS beta-glucoside transporter subunit EIIBCA, lmo2668-65 operon encoding galactitol PTS) were identified. Congruently, these genes were previously identified as negatively regulated by SigL ( Chaturongakul et al., 2011...”
- Proteomic Analysis of Listeria monocytogenes FBUNT During Biofilm Formation at 10°C in Response to Lactocin AL705
Melian, Frontiers in microbiology 2021 - “...caused by bacteriocin interacting with the cytoplasmic membrane. Notably, transport systems such as -glucosidase IIABC (lmo0027), cellobiose (lmo2763), and trehalose (lmo1255) specific PTS proteins were highly overexpressed. In addition, mannose (lmo0098), a specific PTS protein indicating the adaptive response of sessile cells to the bacteriocin, was...”
- “...up-regulated by bacteriocin-treated sessile cells when compared to untreated biofilm. PTS beta-glucoside transporter subunit IIABC (Lmo0027; FC: 123.9) showed a remarkably high expression, whereas other proteins, such as PTS cellobiose transporter subunit IIC (Lmo2763), subunit IIB (Lmo2762), and subunit IIA (Lmo2765), PTS trehalose transporter subunit IIBC...”
- YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes
Cheng, Gut microbes 2021 - “...Yes lmo1255 PTS trehalose-specific enzyme IIBC 2.93 Yes lmo2002 PTS mannose-specific enzyme IIB 2.72 Yes lmo0027 PTS beta-glucosides specific enzyme IIABC 2.55 Yes lmo0022 PTS fructose-specific enzyme IIB 2.24 Yes lmo2685 PTS cellbiose-specific enzyme IIB 2.16 Yes lmo2683 PTS cellbiose-specific enzyme IIB 2.09 Yes lmo2733 PTS...”
- The Combined Effect of Cold and Copper Stresses on the Proliferation and Transcriptional Response of Listeria monocytogenes
Quesille-Villalobos, Frontiers in microbiology 2019 - “...= 20; COG class G) such as genes that encode the phosphotransferase system (PTS; genes lmo0027, lmo0298, lmo0299, lmo1997, lmo2000, lmo2001, lmo2124, lmo2125, lmo2665, lmo2666 and lmo2708) ( Figure 2B and Supplementary Table S2 ). The effect of copper over the carbohydrate metabolism has been described...”
- Interaction with enzyme IIBMpo (EIIBMpo) and phosphorylation by phosphorylated EIIBMpo exert antagonistic effects on the transcriptional activator ManR of Listeria monocytogenes
Zébré, Journal of bacteriology 2015 - “...permease of the glucose class, which is encoded by lmo0027. However, the glucose transport activities of these transporters can be detected only in mutants that...”
- More
- Physiological and transcriptional characterization of persistent and nonpersistent Listeria monocytogenes isolates
Fox, Applied and environmental microbiology 2011 - “...LMOf6854_2061 LMOf6854_0111 LMOf6854_2394 LMOf6854_1066 LMOf6854_2799 LMOf6854_0030 LMOf6854_2784 LMOf6854_2062 manL treB ptsH ptsI LMOf6854_0827 Downregulated...”
- Listeria monocytogenes σA Is Sufficient to Survive Gallbladder Bile Exposure
Boonmee, Frontiers in microbiology 2019 - “...part of an operon that includes genes encoding a PTS fructose transporter subunit and alpha-mannosidase. LMRG_02456 and LMRG_02567 encode PTS system beta-glucoside-specific components. LMRG_01933 encodes PTS system cellobiose-specific IIB component. LMRG_01669-LMRG_01673 is an operon encoding genes involved in myo-inositol degradation and LMRG_01535 is a phage capsid...”
- Genome-wide identification of Listeria monocytogenes CodY-binding sites
Biswas, Molecular microbiology 2020 - “...124 3 UPSTREAM 156.7 yes 12. LMRG_RS08950,LMRG_RS08945 purC,purS 647 78 8 INTERNAL,UPSTREAM 140.0 yes 13. LMRG_RS00135 110 6 INTERNAL 116.8 yes 14. LMRG_RS02565/LMRG_RS02570 176 186 ND UPSTREAM/UPSTREAM 115.1 no 14. LMRG_RS02565/LMRG_RS02570 276 91 16 UPSTREAM/UPSTREAM 115.1 yes 14. LMRG_RS02570 86 17 INTERNAL 115.1 no 15. LMRG_RS02730...”
- Transcriptome analysis of Listeria monocytogenes exposed to biocide stress reveals a multi-system response involving cell wall synthesis, sugar uptake, and motility
Casey, Frontiers in microbiology 2014 - “...phosphotransferase system (PTS) (HPr protein) 4.33 LM6179_1334 ptsG Putative PTS system, glucose-specific, IIA component 11.72 LM6179_0306 bglP PTS system beta-glucoside-specific EIIBCA component 27.79 LM6179_0618 bglH aryl-phospho-beta-d-glucosidase 11.11 LM6179_2766 LM6179_2766 PTS system protein 69.14 LM6179_2769 LM6179_2769 PTS system mannose/fructose/sorbose family IID subunit 66.88 LM6179_2770 LM6179_2770 PTS system...”
CA_C0570 glucose PTS transporter subunit IIA from Clostridium acetobutylicum ATCC 824
CAC0570 PTS enzyme II, ABC component from Clostridium acetobutylicum ATCC 824
47% identity, 9% coverage
- Alleviation of Carbon Catabolite Repression through araR and xylR Inactivation in Clostridium acetobutylicum DSM 792
Delarouzée, Applied and environmental microbiology 2023 (secret) - Clostridium acetobutylicum Biofilm: Advances in Understanding the Basis
Zhang, Frontiers in bioengineering and biotechnology 2021 - “...TABLE 1 Some genes/proteins differentially regulated in C. acetobutylicum biofilms*. Genes/proteins General change Description Glycolysis CA_C0570 (glcG) Up-regulated PTS sugar transporter subunit CA_C3657 (gapN) Up-regulated NADP + -dependent Glyceraldehyde-3-phosphate dehydrogenase CA_C0712 (pgm) Up-regulated Phosphoglycerate mutase CA_C0713 (eno) Up-regulated Enolase CA_P0141-0142, CA_C0809-0810 Up-regulated Hydrogenases and maturation factors...”
- Fermentation of oxidized hexose derivatives by Clostridium acetobutylicum
Servinsky, Microbial cell factories 2014 - “...adjacent to arrows represent the following enzyme activities and their corresponding genes. 1) glucose PTS, CA_C0570; 2) phosphoglucose isomerase, CA_C2680; 3) phosphofructokinase, CA_C0517; 4) fructose-bis-P aldolase, CA_C0827, CA_P0064; 5) triosephosphate isomerase, CA_C0711; 6) glyceradehyde-3-P dehydrogenase, CA_C0709; 7) phosphoglycerate kinase, CA_C0710; 8) phosphoglycerate mutase, CA_C0712, CA_C2741, CA_C3021;...”
- Transcriptional analysis of micronutrient zinc-associated response for enhanced carbohydrate utilization and earlier solventogenesis in Clostridium acetobutylicum
Wu, Scientific reports 2015 - “...medium at the exponential growth phase of 16h without/with supplementary zinc. Correspondingly, the gene glcG (CAC0570) encoding a glucose-specific PTS was significantly upregulated accompanied with the other two genes CAC1353 and CAC1354 for glucose transport in the presence of zinc. Additionally, genes involved in the metabolisms...”
- “...for two glucose PTS were significantly influenced by supplementary zinc. The expression of gene glcG (CAC0570), typically belonging to the Glc family and encoding glucose PTS IICBA, was 3.62-fold upregulated. Additionally, the expression of the other two genes CAC1353 and CAC1354 encoding the putative glucose PTS...”
- Complex and extensive post-transcriptional regulation revealed by integrative proteomic and transcriptomic analysis of metabolite stress response in Clostridium acetobutylicum
Venkataramanan, Biotechnology for biofuels 2015 - “...21 , 22 ]. Several ABC transporters and permeases (Fig. 2c ) (CAC3262, CAC0662, CAC0272, CAC0570, CAC0108, CAC0107) for the transport of sugars, amino acids, and peptides were found to be translated under butanol stress but not under the control condition. These data suggest that the...”
- Pleiotropic functions of catabolite control protein CcpA in Butanol-producing Clostridium acetobutylicum
Ren, BMC genomics 2012 - “...COA-transferase subunit B 0.27 0.170.01 1.00 0.240.06 CAC0103 cysC Adenylylsulfate kinase 0.25 0.040.01 182 49.1441.18 CAC0570 glcG PTS enzyme II, ABC component 0.16 0.050.02 0.19 0.340.14 CAC0310 abrB310 Stationary/sporulation gene regulator 0.14 0.620.08 0.18 0.590.22 CAC1341 araD l -ribulose-5-phosphate 4-epimerase 2.29 2.110.50 5.81 2.331.80 CAC1343 ptk...”
- “...]. Therefore, this gene cluster is likely responsible for fructose metabolism. In contrast, gene glcG (CAC0570), CAC1353 and CAC1354, encoding d -glucose PTS enzyme II components, were all significantly down-regulated after ccpA inactivation (Additional file 5 : Table S4 and Figure 3 ). This observation suggested...”
- Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum
Amador-Noguez, Applied and environmental microbiology 2011 - “...included the following: glucose phosphotransferase system (PTS), cac0570, cac2995, and cac3427; fumarate hydratase, cac3091; valine (Val), leucine (Leu), and...”
- “...solventogenesis. The expression profile of glucose PTS genes (cac0570, cac2995, and cac3427) correlates with the drop in the levels of the upper glycolysis...”
- Confirmation and elimination of xylose metabolism bottlenecks in glucose phosphoenolpyruvate-dependent phosphotransferase system-deficient Clostridium acetobutylicum for simultaneous utilization of glucose, xylose, and arabinose
Xiao, Applied and environmental microbiology 2011 - “...To attenuate glucose PTS activity in cells, gene glcG (cac0570), which was suggested by bioinformatics analysis to encode the PTS enzyme II (45), was disrupted...”
BSQ49_09740 beta-glucoside-specific PTS transporter subunit IIABC from Liquorilactobacillus hordei
43% identity, 12% coverage
UH47_06270 glucose-specific PTS transporter subunit IIBC from Staphylococcus pseudintermedius
38% identity, 10% coverage
- Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius
Magnowska, Scientific reports 2019 - “...90 90 30 90 90 Central metabolism UH47_06750 PTS system glucose-specific transporter subunit 1 glycolysis UH47_06270 PTS system glucose-specific transporter subunit 1.2 glycolysis UH47_12920 glyceraldehyde-3-phosphate dehydrogenase 2.1 1.5 glycolysis/gluconeogenesis UH47_11065 phosphoglyceromutase gpmI 1.3 glycolysis/gluconeogenesis UH47_11070 triosephosphate isomerase tpi 1.2 1.5 1.3 glycolysis/gluconeogenesis UH47_11075 phosphoglycerate kinase pgk...”
- “...and consistent alterations were observed for carbohydrate uptake systems. Glucose uptake by the glucose-specific transporter (UH47_06270, UH47_06750) was downregulated, whereas expression of numerous alternative carbohydrate carbon source uptake and activation proteins were triggered, i.e. trehalose (permease, UH47_02750; 6-phosphate hydrolase, treC ), glucoside (specific transporter, UH47_02965), glucarate...”
Cbei_2833 PTS system, beta-glucoside-specific IIABC subunit from Clostridium beijerincki NCIMB 8052
59% identity, 8% coverage
LP_RS14755 beta-glucoside-specific PTS transporter subunit IIABC from Lactiplantibacillus plantarum WCFS1
48% identity, 10% coverage
SAK_0915 PTS system, beta-glucoside-specific IIABC component from Streptococcus agalactiae A909
52% identity, 9% coverage
- The Role of Regulator Catabolite Control Protein A (CcpA) in Streptococcus agalactiae Physiology and Stress Response
Roux, Microbiology spectrum 2022 - “...PTS system, IID component, mannose/fructose/sorbose family; SAK_0528-0530 , galactitol PTS, IIA, IIC, and IIB subunits; SAK_0915 , PTS system, -glucoside-specific IIABC component; SAK_1377 , PTS system, fructose-specific IIABC component; SAK_1702 , PTS system, sucrose-specific IIABC component; SAK_1893-1895 , PTS system, glucose-specific IIABC component; SAK_1908-1911 , mannose/fructose/sorbose...”
- The sensor histidine kinase RgfC affects group B streptococcal virulence factor expression independent of its response regulator RgfA
Gendrin, Infection and immunity 2015 - “...SAK_0399, SAK_1909, SAK_0398, SAK_0400, SAK_0523, SAK_ 0529, SAK_0915, SAK_0530, SAK_0257, and SAK_1920). Increased expression of such genes may facilitate GBS...”
ID870_00140 PTS transporter subunit IIBC from Streptococcus agalactiae CJB111
SAK_1920 PTS system, glucose-specific IIABC component, putative from Streptococcus agalactiae A909
54% identity, 7% coverage
- Genomic Analyses Identify Manganese Homeostasis as a Driver of Group B Streptococcal Vaginal Colonization
Burcham, mBio 2022 - “...Zinc ABC transporter permease 6.37 ID870_08780 Putative metal homeostasis protein 10.07 Transport systems Phosphotransferase systems ID870_00140 PTS transporter subunit EIIC 4.92 ID870_00190 PTS sugar transporter subunit IIB 9.85 ID870_00265 PTS sugar transporter subunit IIA 6.35 7.73 8.63 5.88 ID870_00430 PTS system mannose/fructose/ N -acetylgalactosamine-transporter subunit IIB...”
- The Role of Regulator Catabolite Control Protein A (CcpA) in Streptococcus agalactiae Physiology and Stress Response
Roux, Microbiology spectrum 2022 - “...of 1 and 1. The 14 selected genes are rbsR ( SAK_0171 ), ptsG ( SAK_1920 ), glpK ( SAK_0345 ), pyk ( SAK_1037 ), ptsK ( SAK_0862 ), ptsI ( SAK_0946 ), budB ( SAK_1279 ), pfkA ( SAK_1036 ), ldhA ( SAK_0821 ), adhP...”
- “...platform ( https://www.genoscope.cns.fr/agc/microscope ) ( 90 ) as explained in Materials and Methods. ptsG ( SAK_1920 ), PTS system, glucose-specific IIABC component; SAK_0257 , PTS system, trehalose-specific IIABC component; SAK_0398-0400 , lactose/cellobiose PTS, IIA, IIB, and IIC subunits; mannose/fructose/sorbose PTS, IID, IIC, IIB, and IIA subunits;...”
- A Vaginal Tract Signal Detected by the Group B Streptococcus SaeRS System Elicits Transcriptomic Changes and Enhances Murine Colonization
Cook, Infection and immunity 2018 - “...sak_1894 sak_1895 sak_1908 sak_1909 sak_1910 sak_1911 sak_1920 ABC transporters sak_0166 sak_0167 sak_0168 sak_0169 sak_0207 sak_0208 sak_0209 sak_0210 sak_0302...”
- “...sak_1188 sak_0258 sak_1155 sak_0398 sak_0399 sak_0400 sak_1920 sak_1702 sak_0354 sak_0257 Fatty acid biosynthesis and metabolism sak_0416 sak_0417 sak_0418...”
- The sensor histidine kinase RgfC affects group B streptococcal virulence factor expression independent of its response regulator RgfA
Gendrin, Infection and immunity 2015 - “...kinase, rgfB shows homology to nucleases, and SAK_1920 encodes a putative glucose-specific phosphotransferase system (PTS) transporter. In GBS A909, RgfA is...”
- “...SAK_0523, SAK_ 0529, SAK_0915, SAK_0530, SAK_0257, and SAK_1920). Increased expression of such genes may facilitate GBS survival and nutrient acquisition during...”
CD3097 PTS system, IIabc component from Clostridium difficile 630
54% identity, 8% coverage
RBAM_020380 YpqE from Bacillus amyloliquefaciens FZB42
58% identity, 29% coverage
- Differential proteomics research of Bacillus amyloliquefaciens and its genome-shuffled saltant for improving fengycin production
Zhao, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 2018 - “...+2.5326 697 Phosphomethylpyrimidine kinase gi|154685606 YP_001420767 yjbV 29037/5.93 28436/5.53 211 52 +2.5372 705 Hypothetical protein RBAM_020380 gi|154686470 YP_001421631 ypqE 17961/5.22 16367/5.13 80 22 +65.1469 a Spot numbers assigned by the software refer to the proteins labeled in Fig. 1 . b Protein name in the National...”
- “...RBAM_014930 Cytoplasmic 577 Hypothetical protein RBAM_028130 Cytoplasmic 685 Hypothetical protein RBAM_003150 Cytoplasmic 705 Hypothetical protein RBAM_020380 Cytoplasmic a Spot numbers assigned by the software refer to the proteins labeled in Fig. 1 . b Protein name in the National Center for Biotechnology Information (NCBI) database for...”
gbs1946 Unknown from Streptococcus agalactiae NEM316
SAG1959 PTS system, IIABC components from Streptococcus agalactiae 2603V/R
54% identity, 7% coverage
- Whole-Genome Comparison Uncovers Genomic Mutations between Group B Streptococci Sampled from Infected Newborns and Their Mothers
Almeida, Journal of bacteriology 2015 - “...gbs0198 SAG0163_02655 gbs0476 (3= UTR) gbs1259 gbs1986 gbs1427 gbs1950 gbs1946 (5= UTR) gbs0668 gbs1038 gbs1946 (5= UTR) gbs1377 SNP SNP SNP Indel SNP SNP Indel...”
- “...a glucose-specific phosphotransferase system (PTS) enzyme IIABC gene (gbs1946 [ptsG]) that takes part in the phosphorylation and transport of glucose (55). In...”
- Transcriptome adaptation of group B Streptococcus to growth in human amniotic fluid
Sitkiewicz, PloS one 2009 - “...family IIB component gbs1939 - 11.1 34.6 42.6 PTS system, mannose fructose family IIA component gbs1946 5.8 4.6 PTS system, glucose-specific IIABC component gbs2116 - 3.2 2.3 Glucose uptake family protein Values represent fold change in expression in amniotic fluid compared to expression in THY; ML,...”
- CsrRS and environmental pH regulate group B streptococcus adherence to human epithelial cells and extracellular matrix
Park, Infection and immunity 2012 - “...sag1463 (sal1543) Rga 33 sag1490 sag1792 sag1957, sag1958, sag1959 RovS DltR RgfA, RgfB, RgfC Regulation of binding to keratin 4, fibrinogen, and epithelial...”
- “...RgfB 1341 1342 F R TGAAGAGCTGGTGTACCTTCAA TAGCAGAGCATATCTTCCGTGA sag1959 RgfC 1346 1345 F R AGTGTCACTCCAGCACGTTTTA GTGTCACCCAAACAAAGTTGAA sag1462 Srr1 1316 1315...”
SPD_0661 PTS system, IIABC components from Streptococcus pneumoniae D39
spr0668 PTS glucose-specific enzyme IIABC component from Streptococcus pneumoniae R6
52% identity, 7% coverage
- In vivo proteomics identifies the competence regulon and AliB oligopeptide transporter as pathogenic factors in pneumococcal meningitis
Schmidt, PLoS pathogens 2019 - “...Similar, other transporters were not affected with the exception of the PTS system transporter protein SPD_0661 (2-fold) and a potential ABC iron-transporter system encoded by the operon 804. This operon encodes the proteins SPD_1606 to SPD_1610 with the lipoprotein SPD_1609 as SBP of the system [...”
- Interplay Between Capsule Expression and Uracil Metabolism in Streptococcus pneumoniae D39
Carvalho, Frontiers in microbiology 2018 - “...differentially expressed genes (Table S3 ). Noteworthy, eight PTS uptake systems ( spd_0066-9, spd_0502, spd_0559-61, spd_0661, spd_1047-8, spd_1057, spd_1959 , and spd_1992-89 ), and the enzymes involved in the metabolism of the PTS-incoming sugars ( spd_0063, spd_0065, spd_0070-1, spd_0503, spd_0562, spd_1046, spd_1958-7 , and spd_1995-4 )...”
- Maltose-Dependent Transcriptional Regulation of the mal Regulon by MalR in Streptococcus pneumoniae
Afzal, PloS one 2015 - “...PTS system, IIC component, CelD 3.2 spd_0311 Glucan 1,6-alpha-glucosidase, DexB 3.4 spd_0580 Glucokinase, RokB 2.4 spd_0661 PTS system, IIABC components, PtsG 4.6 spd_0662 Hypothetical protein 3.0 spd_1215 Alpha-amylase, AmyA2 6.6 spd_1932 Glycogen phosphorylase family protein, MalP 6.2 spd_1933 Amylomaltase, MalM 6.2 spd_1934 Maltose/maltodextrin ABC transporter, MalX...”
- “...D39 Tag b Function c Ratio spd_0311 Glucan 1,6-alpha-glucosidase, DexB 2.2 spd_0580 Glucokinase, RokB 3.0 spd_0661 PTS system, IIABC components, PtsG 1.8 spd_0662 Hypothetical protein 3.7 spd_1215 Cytoplasmic alpha-amylase, AmyA2 6.8 spd_1933 Amylomaltase, MalM 6.3 spd_1934 Maltose/maltodextrin ABC transporter, MalX 2.9 spd_1938 Maltose operon transcriptional repressor,...”
- Transcriptional and metabolic effects of glucose on Streptococcus pneumoniae sugar metabolism
Paixão, Frontiers in microbiology 2015 - “...1.26 SPD_0560 PTS system, IIB component, putative 1.21 SPD_0561 PTS system, IIC component, putative 1.55 SPD_0661 exp5 PTS system, IIABC components 0.61 SPD_0773 PTS system, fructose specific IIABC components 0.36 SPD_1039 ptsI Phosphoenolpyruvate-protein phosphotransferase 0.49 SPD_1040 ptsH Phosphocarrier protein HPr 0.56 SPD_1047 lacE-2 PTS system, lactose-specific...”
- A random six-phase switch regulates pneumococcal virulence via global epigenetic changes
Manso, Nature communications 2014 - “...insertion of linear PCR fragments during transformation ( Supplementary Fig. 2f ), the mutant gene SPD_0661 containing a kanamycin cassette ( aphIII ) (ref. 28 ) was amplified using the primers 5-CGGTAAGGCTTTGATGGTAGTTA-3 and 5-GGTTTACCTTCAAGACTTACTGTG-3. This fragment contained one SpnD39IIIA recognition site. Modified fragments were constructed with...”
- CcpA ensures optimal metabolic fitness of Streptococcus pneumoniae
Carvalho, PloS one 2011 - “...transporter in Streptococcaceae [3] , was among them. Carbohydrate-specific CcpA-mediated regulation was also in effect: SPD_0661, a Glc-family PTS system, was exclusively repressed in Gal-CDM. This gene is predicted to be a direct target of CcpA due to the presence of a cre site in its...”
- iDS372, a Phenotypically Reconciled Model for the Metabolism of Streptococcus pneumoniae Strain R6
Dias, Frontiers in microbiology 2019 - “...that acetate is a by-product of N -acetyl- D -glucosamine degradation. In the model, genes spr0668 and spr1528 encoding phosphoenolpyruvate-dependent phosphotransferase sugar-specific systems transport this compound into the cell converting it to N -acetyl- D -glucosamine-6-phosphate and releasing pyruvate. Then, gene spr1867 encoding an N -acetyl-...”
- A functional genomics approach to establish the complement of carbohydrate transporters in Streptococcus pneumoniae
Bidossi, PloS one 2012 - “...beta-glucosides [55] SP0645-6-7 SPG00585-6-7 spr0562-3-4 PTS-Gat gatABC conserved galactose galactose, lactose, galactitol [56] SP0758 SPG00690 spr0668 PTS-Glc malT conserved maltose, maltotriose, maltodextrin and glycogen maltose [58] [60] SP0845-6-7-8 SPG00765-6-7-8 spr0747-8-9-50 CUT2 conserved ribonucleoside [79] SP0877 SPG00801 spr0780 PTS-Fru fruA conserved fructose fructose [63] SP1185-6 SPG01082-3 spr1070-1...”
SP_0758 PTS transporter subunit IIBC from Streptococcus pneumoniae TIGR4
52% identity, 7% coverage
- Targeted Transcriptomic Screen of Pneumococcal Genes Expressed during Murine and Human Infection
Basset, Infection and immunity 2022 (secret) - Polyamine Synthesis Effects Capsule Expression by Reduction of Precursors in Streptococcus pneumoniae
Ayoola, Frontiers in microbiology 2019 - “...glgA SP_1124 Glycogen synthase 1.7 malP SP_2106 Maltodextrin phosphorylase 2.1 malQ SP_2107 4-Alpha-glucanotransferase 2.8 exp5 SP_0758 PTS system glucose-specific EIICBA component 1.6 SP_1795 SP_1795 Putative sucrose-6-phosphate hydrolase 3.0 glmS SP_0266 Glutamine-fructose-6-phosphate aminotransferase 1.6 nagA SP_2056 N -acetylglucosamine-6-phosphate deacetylase 2.9 nagB SP_1415 Glucosamine-6-phosphate deaminase 3.9 pyrR SP_1278...”
- MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis
Tan, PloS one 2015 - “...35 , 36 ]. In S . pneumoniae , the MalXCD transporter and MalT homolog (SP_0758) undertake this process [ 23 ]. while the MalT homolog (SSUSC84_0343) may be responsible for uptake of maltose and maltotrise in S . suis . Though the results of qRT-PCR,...”
- The molecular basis of glycogen breakdown and transport in Streptococcus pneumoniae
Abbott, Molecular microbiology 2010 - “...S. pneumoniae TIGR4 encodes a putative protein (locus tag SP_0758) with 70% amino acid sequence identity to MalT. Based on this similarity, we postulate SP_0758...”
- “...based on their predicted functions. The role of SP_0758, the MalT homologue, is as a putative PTS component. The putative transported products of SP_0758...”
SSA_1752 Phosphotransferase system, trehalose-specific IIBC component, putative from Streptococcus sanguinis SK36
56% identity, 8% coverage
ID870_01290 sucrose-specific PTS transporter subunit IIBC from Streptococcus agalactiae CJB111
46% identity, 11% coverage
CD2512 PTS system, IIa component from Clostridium difficile 630
48% identity, 35% coverage
- Grad-seq identifies KhpB as a global RNA-binding protein in Clostridioides difficile that regulates toxin production
Lamm-Schmidt, microLife 2021 - “...probes specific for the 5-UTR regions of bglF (CDIF630nc_008, PTT), CD0426 (CDIF630nc_010, PTT), CD1541 and CD2512. To extend on this theme, we observed the 10.1kDa protein CD3568 (Q181H0) to sediment in ribosome-associated fractions. CD3568 belongs to the broadly conserved Veg domain protein family (PF06257) with members...”
- “...UTR candidates include the 5UTRs of CD1541 (putative drug/sodium antiporter of the MATE family) and CD2512 (PTS system, maltose-specific IIA component). Northern blotting of gradient fractions detected stable transcripts for all 5UTRs that formed LMW complexes (Fig. 2C ). While most of them likely represent stable...”
- Increased sporulation underpins adaptation of Clostridium difficile strain 630 to a biologically-relevant faecal environment, with implications for pathogenicity
Ternan, Scientific reports 2018 - “...as were genes encoding the IIA and IIB components of the glucose PTS transport system (CD2512, CD2510, respectively). Expression of the IIC and IID components of the mannose/fructose/sorbose transport system (CD3277, CD3276) were increased by 4 and 6.6fold, respectively. Conversely, expression of PTS system components associated...”
- Integration of metabolism and virulence by Clostridium difficile CodY
Dineen, Journal of bacteriology 2010 - “...(oppBCADF) CD0875-CD0873 CD0878-CD0876 CD0879 CD1259-CD1260 CD2511-CD2508 CD2512 CD2702 (brnQ) CD3615 CD3616 CD3628 Amino acid transporter Cobalt transporter...”
gbs1734 Unknown from Streptococcus agalactiae NEM316
46% identity, 11% coverage
- Transcriptome adaptation of group B Streptococcus to growth in human amniotic fluid
Sitkiewicz, PloS one 2009 - “...facilitator protein gbs1714 3.9 Pyruvate,phosphate dikinase gbs1732 pmi 3.2 Mannose-6-phosphate isomerase gbs1733 scrK 6.9 Fructokinase gbs1734 scrA 22.3 2.6 PTS system, sucrose-specific IIABC component gbs1735 scrB 5.2 Sucrose-6-phosphate hydrolase gbs1777 glpF.2 2.2 3.8 Glycerol uptake facilitator protein gbs1797 2.3 Galactose-1-phosphate uridylyltransferase gbs1811 plr 2.1 2.5 Glyceraldehyde...”
LLNZ_07350 glucose PTS transporter subunit IIA from Lactococcus cremoris subsp. cremoris NZ9000
llmg_1426 sucrose-specific PTS system IIBC component from Lactococcus lactis subsp. cremoris MG1363
56% identity, 8% coverage
- Genomics and transcriptomics analysis reveals the mechanism of isobutanol tolerance of a laboratory evolved Lactococcus lactis strain
Gupta, Scientific reports 2020 - “...transcripts calculated from RNA-seq and RT-qPCR. S. No Locus_tag Annotation LogFC RNA-seq LogFC RT-qPCR 1 LLNZ_07350 Sucrose specific PTS system IIBC component 5.48 2 2 LLNZ_02240 Carbon starvation protein A 4.3 2.45 3 LLNZ_09555 Multidrug resistance ABC transporter ATP-binding and permease protein 2.84 4.1 5 LLNZ_05785...”
- “...synthase Arginine biosynthesis 7 LLNZ_06600 Acetolactate synthase catalytic subunit Valine, leucine and isoleucine biosynthesis 8 LLNZ_07350 Sucrose-specific PTS system IIBC component Phosphotransferase system (PTS) 9 LLNZ_01385 Fructose-1,6-bisphosphatase class 3 Glycolysis/gluconeogenesis 10 LLNZ_05080 Phosphoribosylaminoimidazole synthetase Purine metabolism 11 LLNZ_10425 ABC-type oligopeptide transport system, periplasmic component Quorum sensing...”
- Adaptation of Lactococcus lactis to high growth temperature leads to a dramatic increase in acidification rate
Chen, Scientific reports 2015 - “...llmg_1474 1.4 9.9E-06 Putative voltage gated chloride channel comGC 1.4 2.6E-08 Putative competence protein ComGC llmg_1426 1.5 3.0E-06 Sucrose-specific PTS system IIBC component comGA 1.6 5.5E-09 Putative competence protein ComGA cbiQ 1.6 3.3E-09 Putative cobalt ABC transporter permease protein arcD1 1.7 4.7E-07 Arginine/ornithine antiporter nha 2.0...”
- Towards enhanced galactose utilization by Lactococcus lactis
Neves, Applied and environmental microbiology 2010 - “...llmg_0866 llmg_0867 llmg_0963 llmg_1045 (ptbA) llmg_1244 llmg_1426 (yleD) llmg_1568 (fruA) llmg_pseudo54 (yidB) Familya VOL. 76, 2010 GALACTOSE METABOLISM...”
SAG1690 PTS system, IIABC components from Streptococcus agalactiae 2603V/R
46% identity, 11% coverage
- Adaptive response of neonatal sepsis-derived Group B Streptococcus to bilirubin
Hansen, Scientific reports 2018 - “...transporter ATP-binding protein 2.02 3.37E-04 4.60E-02 SAG1441 Maltose/maltodextrin ABC transporter maltose/maltodextrin-binding protein 2.19 4.69E-04 5.91E-02 SAG1690 PTS system transporter subunit IIABC 2.01 5.22E-04 5.91E-02 SAG2072 Uridine phosphorylase 1.70 5.26E-04 5.91E-02 SAG0331 Formate acetyltransferase 1.65 8.21E-04 8.43E-02 SAG2014 Hypothetical protein 1.17 8.39E-04 8.43E-02 Genes encoding transport proteins...”
CHF17_RS08755 sucrose-specific PTS transporter subunit IIBC from Streptococcus agalactiae
46% identity, 11% coverage
WP_002581229 PTS sugar transporter subunit IIA from Clostridium butyricum
45% identity, 37% coverage
LP_RS13505 sucrose-specific PTS transporter subunit IIBC from Lactiplantibacillus plantarum WCFS1
lp_3219 sucrose PTS, EIIBCA from Lactobacillus plantarum WCFS1
50% identity, 9% coverage
PFREUD_01380 glucose PTS transporter subunit IIA from Propionibacterium freudenreichii subsp. shermanii CIRM-BIA1
43% identity, 7% coverage
LPST_C2650 sucrose-specific PTS transporter subunit IIBC from Lactiplantibacillus plantarum ST-III
50% identity, 9% coverage
- The Carbohydrate Metabolism of Lactiplantibacillus plantarum
Cui, International journal of molecular sciences 2021 - “...catalysis of 6-phosphate sucrose hydrolase to produce 6-phosphate-glucose and fructose. Furthermore, another gene cluster sacPTS26 (LPST_C2650 to LPST_C2652) was found in ST-III strain, and was also involved in the utilization of FOS, was composed of pts26BCA , agl4 and sacR2 [ 40 ]. The genomic analysis...”
bglP / CAA84286.1 beta-glucoside permease from Bacillus subtilis (see paper)
57% identity, 8% coverage
TC 4.A.1.2.11 / P40739 Aryl β-glucoside porter, IIBCA (BglP; SytA) (35% identical to 4.A.1.2.2) from Bacillus subtilis (see 5 papers)
57% identity, 8% coverage
Q831R2 PTS system, IIA component from Enterococcus faecalis (strain ATCC 700802 / V583)
EF2438 PTS system, IIA component from Enterococcus faecalis V583
61% identity, 25% coverage
BMMGA3_RS01615 glucose-specific PTS transporter subunit IIBC from Bacillus methanolicus MGA3
46% identity, 10% coverage
SAK_1702 PTS system, sucrose-specific IIABC component from Streptococcus agalactiae A909
46% identity, 11% coverage
- The Role of Regulator Catabolite Control Protein A (CcpA) in Streptococcus agalactiae Physiology and Stress Response
Roux, Microbiology spectrum 2022 - “...subunits; SAK_0915 , PTS system, -glucoside-specific IIABC component; SAK_1377 , PTS system, fructose-specific IIABC component; SAK_1702 , PTS system, sucrose-specific IIABC component; SAK_1893-1895 , PTS system, glucose-specific IIABC component; SAK_1908-1911 , mannose/fructose/sorbose PTS, IID, IIC, IIB, and IIA subunits, SAK_0532-0534 , sugar ABC transporter; SAK_1475-1477 ,...”
- A Vaginal Tract Signal Detected by the Group B Streptococcus SaeRS System Elicits Transcriptomic Changes and Enhances Murine Colonization
Cook, Infection and immunity 2018 - “...sak_0525 sak_0526 sak_0528 sak_0529 sak_0530 sak_1377 sak_1702 sak_1759 sak_1825 sak_1833 sak_1834 sak_1835 sak_1893 sak_1894 sak_1895 sak_1908 sak_1909...”
- “...sak_0258 sak_1155 sak_0398 sak_0399 sak_0400 sak_1920 sak_1702 sak_0354 sak_0257 Fatty acid biosynthesis and metabolism sak_0416 sak_0417 sak_0418 sak_0419...”
spr1699 Phosphotransferase system, trehalose-specific IIBC component from Streptococcus pneumoniae R6
58% identity, 7% coverage
SPD_1664 PTS system, trehalose-specific IIABC components from Streptococcus pneumoniae D39
58% identity, 8% coverage
- StkP- and PhpP-Mediated Posttranslational Modifications Modulate the S. pneumoniae Metabolism, Polysaccharide Capsule, and Virulence
Kant, Infection and immunity 2023 - “...SPD_1935 Sugar ABC transporter permease 2.43 8.81E-47 1.96 8.43E-27 SPD_1663 Alpha,alpha-phosphotrehalase 27.30 9.72E-40 2.15 1.69E-23 SPD_1664 PTS beta-glucoside transporter subunit EIIBCA 8.25 0 8.11 4.12E-261 SPD_RS4270 Hypothetical protein 2.32 5.6E_03 2.12 8.9E-03 SPD_RS6165 ABC transporter ATP-binding protein 2.43 5.72E-89 2.0 6.87E-47 Miscellaneous genes SPD_632 Bifunctional phosphomethylpyrimidine...”
- SPD_1495 Contributes to Capsular Polysaccharide Synthesis and Virulence in Streptococcus pneumoniae
Zheng, mSystems 2020 - “...that many upregulated proteins in D39 spd1495 , such as LacB, LacD, DexB, BglA-1, SPD_0559, SPD_1664, and SPD_0412, formed a small network. These proteins are involved in carbohydrate metabolism, which supplies some of the key raw materials for CPS synthesis, such as ATP and NAD +...”
- S1 Domain RNA-Binding Protein CvfD Is a New Posttranscriptional Regulator That Mediates Cold Sensitivity, Phosphate Transport, and Virulence in Streptococcus pneumoniae D39
Sinha, Journal of bacteriology 2020 (secret) - Redefining the Small Regulatory RNA Transcriptome in Streptococcus pneumoniae Serotype 2 Strain D39
Sinha, Journal of bacteriology 2019 - “...spd_1577 spd_1580, spd_1582 spd_1605, spd_1604 spd_1662, murI spd_1664, treC Spd_sr 39c Spd_sr 52c Spd_sr91 Spd_sr92 Spd_sr93 Spd_sr94 Spd_sr95 Spd_sr10c...”
- Deep genome annotation of the opportunistic human pathogen Streptococcus pneumoniae D39
Slager, Nucleic acids research 2018 - “...G>T R28L (CGC>CTC) 1672084 1672541 SPD_1660 ( rdgB ) G>A RdgB T117I (ACA>ATA) 1676516 1676973 SPD_1664 ( treP ) C>T TreP G359D (GGC>GAC) 1787708 1788165 SPD_1793 C>T A2V (GCA>GTA) 1977728 1978185 SPD_2002 ( dltD ) C>A DltD V252F (GTC>TTC) 2022372 2022829 SPD_2045 ( mreC ) A>G...”
- N-acetylglucosamine-Mediated Expression of nagA and nagB in Streptococcus pneumoniae
Afzal, Frontiers in cellular and infection microbiology 2016 - “...spd_1049 Transcription antiterminator, LacT 4.4 spd_0282 Hypothetical protein 4.4 spd_0281 PTS system, IIA component 4.2 spd_1664 PTS system, trehalose-specific IIABC components 4.2 spd_1663 -phosphotrehalase, TreC 4.0 spd_0279 PTS system, IIB component 3.9 spd_1495 Sugar ABC transporter, sugar-binding protein 3.7 spd_1973 -1,2-mannosidase, putative 3.6 spd_1496 PTS system,...”
- Ascorbic acid-dependent gene expression in Streptococcus pneumoniae and the activator function of the transcriptional regulator UlaR2
Afzal, Frontiers in microbiology 2015 - “...triad protein A, PhtA 3.9 spd_1053 Galactose-6-phosphate isomerase, LacA subunit, LacA 2.5 spd_1295 Hemolysin 4.6 spd_1664 PTS system, trehalose-specific IIABC components 2.1 spd_1839 Transketolase, Tkt 4.7 spd_1840 L-ascorbate 6-phosphate lactonase, UlaG 35.5 spd_1841 Transcriptional regulator, UlaR 14.9 spd_1842 L-ribulose-5-phosphate 4-epimerase, UlaF/AraD 11.6 spd_1843 L-xylulose 5-phosphate 3-epimerase,...”
SP_1884 trehalose PTS system, IIABC components from Streptococcus pneumoniae TIGR4
58% identity, 8% coverage
- A bacterial pan-genome makes gene essentiality strain-dependent and evolvable
Rosconi, Nature microbiology 2022 - “...constructed in a PG06-SP_1205 knockout. The identification of three functionally related suppressive SNPs (SP_1176 A17P, SP_1884 I34T and SP_1885 H182N) suggests that transport of the disaccharide trehalose (or a similar compound) is a central hub for bypassing SP_1205. Gene SP_1205 has a synthetic lethal interaction with...”
- The Effect of Impaired Polyamine Transport on Pneumococcal Transcriptome
Nakamya, Pathogens (Basel, Switzerland) 2021 - “...for pneumococcal stress responses. Locus Tag Gene Fold Change potABCD /TIGR4 FDR p Value Description SP_1884 SP_1884 3.6 <0.0001 PTS trehalose transporter subunit IIBC SP_1883 SP_1883 3.2 <0.0001 Trehalose 6-phosphate hydrolase SP_1907 groES 3.9 <0.0001 Co-chaperone SP_0519 dnaJ 3.8 <0.0001 Molecular chaperone SP_0517 dnaK 4.2 <0.0001...”
- Polyamine Synthesis Effects Capsule Expression by Reduction of Precursors in Streptococcus pneumoniae
Ayoola, Frontiers in microbiology 2019 - “...ATP-binding protein 1.6 metQ SP_0149 Lipoprotein 1.7 Stress response SP_1883 SP_1883 Putative dextran glucosidase 28.1 SP_1884 SP_1884 Trehalose PTS system, IIABC components 26.2 psaB SP_1648 Manganese ABC transporter, ATP-binding protein 2.7 psaC SP_1649 Putative manganese ABC transporter, permease protein 2.7 psaA SP_1650 Manganese ABC transporter substrate-binding...”
LGAS_1669 Trehalose PTS trehalose component IIBC from Lactobacillus gasseri ATCC 33323
51% identity, 8% coverage
- The PTS transporters of Lactobacillus gasseri ATCC 33323
Francl, BMC microbiology 2010 - “...to inactivate PTS 15, 20, and 21, respectively (Table 5 ). The amplified PTS 15 (LGAS_1669), 20 (LGAS_1778) and 21 (LGAS_1795) internal regions were 819 bp, 760 bp and 675 bp, respectively. The junction fragments for successful pMJM-4 integration were 999 bp and 1039 bp. The...”
TC 4.A.1.2.14 / ART98499 PTS beta-glucoside transporter, EIIBCA of 672 aas and 12 predicted TMSs from Lactobacillus gasseri
51% identity, 8% coverage
- substrates: beta-glucosides
SPCG_RS09625 PTS system trehalose-specific EIIBC component from Streptococcus pneumoniae CGSP14
58% identity, 8% coverage
- The transcriptomic response of Streptococcus pneumoniae following exposure to cigarette smoke extract
Manna, Scientific reports 2018 - “...protein 1.48 0.037 SPCG_RS04895 Endo-beta-N-acetylglucosaminidase LytB 1.40 0.004 SPCG_RS08595 Carbohydrate ABC transporter permease 1.13 0.044 SPCG_RS09625 Phosphotransferase system trehalose transporter subunit IIABC TreP 1.07 0.014 Cell wall/membrane biogenesis SPCG_RS00545 Peptidoglycan-binding protein LysM 1.76 0.004 SPCG_RS04610 ABC-type lipoprotein export system, ATPase component 1.40 0.019 SPCG_RS10170 Membrane protein...”
lmo2772 similar to beta-glucoside-specific enzyme IIABC from Listeria monocytogenes EGD-e
48% identity, 10% coverage
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...lmo2763 PTS cellbiose transporter subunit IIC lmo2765 2.78 Yes/down lmo2765 PTS cellbiose transporter subunit IIA lmo2772 3.43 lmo2772 PTS beta-glucoside transporter subunit IIABC lmo2780 lmo2780 PTS cellbiose transporter subunit IIA lmo2782 4.30 Yes/down lmo2782 PTS cellbiose transporter subunit IIB lmo2783 3.66 Yes/down lmo2783 PTS cellbiose transporter...”
- New Insights into the Lactic Acid Resistance Determinants of Listeria monocytogenes Based on Transposon Sequencing and Transcriptome Sequencing Analyses
Liu, Microbiology spectrum 2023 - “...cellobiose transporter subunit IIC 0.1640 1.13E-16 lmo2765 lmo2765 PTS cellobiose transporter subunit IIA 0.3373 4.58E-02 lmo2772 lmo2772 PTS beta-glucoside transporter subunit IIABC 0.0834 1.54E-13 lmo2782 lmo2782 PTS cellobiose transporter subunit IIB 0.1655 4.48E-02 lmo2787 bvrB beta-Glucoside-specific phosphotransferase enzyme II ABC component 0.2198 3.85E-04 lmo2797 lmo2797 PTS...”
- The Global Regulator CcpA of Listeria monocytogenes Confers Sensitivity to Antimicrobial Fatty Acids
Thomasen, Frontiers in microbiology 2022 - “...792bp in lmo0517 ( lmo0517 ); 744bp in lmo2175 ( lmo2175 ); and 1830bp in lmo2772 ( lmo2772 ), the corresponding primers P1, P2, P3 and P4 ( Supplementary Table S7 ) were used, respectively, for a 2-step PCR amplification of the fragments. CcpA complementation mutant...”
- “...the three most upregulated genes, chosen for mutational analysis, encode a PTS system component ( lmo2772 ) and a hypothetical protein ( lmo0517 ), respectively. Strikingly, these genes were upregulated more than 100-fold in the absence of CcpA ( Supplementary Table S6 ). When going through...”
- YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes
Cheng, Gut microbes 2021 - “...Yes lmo0914 PTS cellbiose-specific enzyme IIB 1.79 Yes lmo2373 PTS beta-glucoside-specific enzyme IIB 1.65 Yes lmo2772 PTS glucose/sucrose-specific enzyme IIB 1.52 Yes lmo1035 PTS beta-glucoside-specific enzyme IIABC 1.42 Yes EIIC lmo2763 PTS cellbiose-specific enzyme IIC 8.80 Yes lmo0298 PTS beta-glucoside-specific enzyme IIC 5.62 Yes lmo2096 PTS...”
- Probing the pan-genome of Listeria monocytogenes: new insights into intraspecific niche expansion and genomic diversification
Deng, BMC genomics 2010 - “...+ 494 lmo2736 Hypothetical protein IIIA + 494 lmo2771 Similar to beta-glucosidase IIIA + - lmo2772 Similar to PTS system, beta-glucoside-specific enzyme IIABC IIIA + - lmo2847 Similar to rhamnulose-1-phosphate aldolase IIIA + 516 lmo2848 Similar to L-rhamnose isomerase IIIA + 516 lmo2849 Similar to rhamnulokinase...”
PECL_1777 N-acetylglucosamine-specific PTS transporter subunit IIBC from Pediococcus claussenii ATCC BAA-344
50% identity, 8% coverage
LMOf2365_2762 PTS system, beta-glucoside-specific, IIABC component from Listeria monocytogenes str. 4b F2365
48% identity, 10% coverage
- Transcriptomics of Listeria monocytogenes Treated With Olive Leaf Extract
Liu, Frontiers in microbiology 2021 - “...the phosphotransferase transport system (PTS), with the expression of 4 genes (LMOf2365_2305, LMOf2365_2664, LMOf2365_2665, and LMOf2365_2762), being differentially regulated at the 3.5-h treatment period (Log 2 FC=5.71, 1.36, 1.47, and 1.13, respectively). At 24h post-treatment, 9 PTS-related genes (LMOf2365_0030, LMOf2365_0113, LMOf2365_0572, LMOf2365_0922, LMOf2365_1272, LMOf2365_2128, LMOf2365_2129, LMOf2365_2130,...”
LEUM_0901 Trehalose PTS trehalose component IIBC from Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
39% identity, 12% coverage
LMOf2365_1056 PTS system, beta-glucoside-specific, IIABC component from Listeria monocytogenes str. 4b F2365
55% identity, 8% coverage
spr1566 Phosphotransferase system enzyme II from Streptococcus pneumoniae R6
49% identity, 8% coverage
SP_1722 sucrose-specific PTS transporter subunit IIBC from Streptococcus pneumoniae TIGR4
49% identity, 8% coverage
SPy_0572 beta-glucoside permease IIABC component from Streptococcus pyogenes M1 GAS
48% identity, 9% coverage
CD2666 PTS system, glucose-specific IIa component from Clostridium difficile 630
45% identity, 45% coverage
- Pleiotropic role of the RNA chaperone protein Hfq in the human pathogen Clostridium difficile
Boudry, Journal of bacteriology 2014 - “...Hfq depletion conditions. Genes encoding two glucose (CD2666, CD2667, CD3027, and CD3030), one mannose (CD3013-CD3015), and one betaglucoside-specific (CD3137)...”
- Global transcriptional control by glucose and carbon regulator CcpA in Clostridium difficile
Antunes, Nucleic acids research 2012 - “...IIC; CD2486CD2488, PTS fructose-like IIC, IIB, IIA; CD3015CD3013, PTS mannose-specific IIA, IIB, IIC; ptsG (CD2667, CD2666), PTS glucose-specific IIA, IICB; pmi (CD2491), mannose-6-phosphate isomerase; rbsBAC (CD0300CD0302), ribose ABC transporter; adhE (CD2966) and CD3006, aldehyde-alcohol dehydrogenase; thlA (CD1059), acetyl-CoA acetyltransferase; hbd (CD1058), 3-hydroxybutyryl-CoA dehydrogenase; crt2 (CD1057), 3-hydroxybutyryl-CoA...”
CD196_2507 PTS system, glucose-specific IIa component from Clostridium difficile CD196
CDR20291_2554 PTS system, glucose-specific IIa component from Clostridium difficile R20291
45% identity, 45% coverage
- Short-term genome stability of serial Clostridium difficile ribotype 027 isolates in an experimental gut model and recurrent human disease
Eyre, PloS one 2013 - “...COG Function SNV site Locus Wildtype SNV Impact Annotation Fig Carbohydrate transport and metabolism 2914411 CD196_2507 C T Truncated PTS system, glucose-specific IIa component 4 Carbohydrate transport and metabolism 3391317 CD196_2881 G T Non-Synonymous PTS system, IIabc component 2,3 Transcription 9921 CD196_0011 A G Non-Synonymous RNA...”
- Biofilm regulation in Clostridioides difficile: Novel systems linked to hypervirulence
Taggart, PLoS pathogens 2021 - “...gene expression in the R20291 luxS mutant biofilm cells revealed 3 genes with increased expression: CDR20291_2554 ( crr ), a PTS system glucose-specific transporter subunit IIA; CDR20291_2927, a cellobiose phosphate-degrading protein; and CDR20291_2930 ( treA ), a trehalose-6-phosphate hydrolase [ 42 ]. A total of 18...”
- Clostridioides difficile LuxS mediates inter-bacterial interactions within biofilms
Slater, Scientific reports 2019 - “...protein 17 CDR20291_1444 1.257181603 phage protein 18 CDR20291_1449 1.008627768 phage tail tape measure protein 19 CDR20291_2554 0.895993497 PTS system glucose-specific transporter subunit IIA 20 CDR20291_2927 1.587075038 cellobiose-phosphate degrading protein 21 CDR20291_2930 1.444846424 trehalose-6-phosphate hydrolase Note: P-adjusted value0.05. To demonstrate the presence of phage in the biofilm,...”
CDR20291_2969 PTS system, IIabc component from Clostridium difficile R20291
CDR20291_2969 beta-glucoside-specific PTS transporter subunit IIABC from Clostridioides difficile R20291
39% identity, 11% coverage
HGB56_08700 glycoside-pentoside-hexuronide (GPH):cation symporter from Lactiplantibacillus plantarum
41% identity, 12% coverage
- The Carbohydrate Metabolism of Lactiplantibacillus plantarum
Cui, International journal of molecular sciences 2021 - “...subunit and small subunit of beta-galactosidase, respectively. The gene HGB56_08695 encodes an alpha-galactosidase. The gene HGB56_08700 encodes an MFS transporter. The locus is present in most L. plantarum strains ( Supplementary Table S3-3 ). Fructose and Sucrose The strain LP-F1 contained multiple phosphotransferase systems (PTSs) responsible...”
lmo1035 similar to phosphotransferase system (PTS) beta-glucoside-specific enzyme IIABC from Listeria monocytogenes EGD-e
55% identity, 8% coverage
- Comparative genomics analysis to explore the biodiversity and mining novel target genes of Listeria monocytogenes strains from different regions
Zhang, Frontiers in microbiology 2024 - “...genes Sequence length/bp Presence profile Encoded protein Product size/bp Source In target In non-target bglF_1 lmo1035 1857 343 (100%) 0 PTS system beta-glucoside-specific EIIBCA component 618 This study davD lmo0913 1,467 343 (100%) 0 Glutarate-semialdehyde dehydrogenase 488 This study menE_1 lmo1672 1,409 343 (100%) 0 O-succinylbenzoic...”
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...lmo1003 lmo1003 Phosphotransferase system enzyme I lmo1017 1.24 Yes/down lmo1017 PTS glucose transporter subunit IIA lmo1035 lmo1035 PTS beta-glucoside transporter subunit IIABC lmo1095 3.29 Yes/down lmo1095 PTS cellbiose transporter subunit IIB lmo1255 1.52 Yes/up 4.14 Yes/down lmo1255 PTS trehalose transporter subunit IIBC lmo1719 1.64 Yes/down lmo1719...”
- YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes
Cheng, Gut microbes 2021 - “...Yes lmo2373 PTS beta-glucoside-specific enzyme IIB 1.65 Yes lmo2772 PTS glucose/sucrose-specific enzyme IIB 1.52 Yes lmo1035 PTS beta-glucoside-specific enzyme IIABC 1.42 Yes EIIC lmo2763 PTS cellbiose-specific enzyme IIC 8.80 Yes lmo0298 PTS beta-glucoside-specific enzyme IIC 5.62 Yes lmo2096 PTS galactitol-specific enzyme IIC 5.35 Yes fruA PTS...”
- Colonisation dynamics of Listeria monocytogenes strains isolated from food production environments
Gray, Scientific reports 2021 - “...study) Fold change Gene COG cat Description KEGG enzyme Isolate and TP # PTS system lmo1035 fig|1639.4014.peg.1355 4.22 G PEP-dependent sugar PTS, EIIA 1 7453 24h lmo1719 fig|1639.4014.peg.1473 4.36 G PTS system cellobiose-specific IIA component 2.7.1.205 lmo0427 fig|1639.4014.peg.2115 4.77 G PTS system, Lactose/Cellobiose specific IIB subunit...”
- Small regulatory RNAs from low-GC Gram-positive bacteria
Brantl, RNA biology 2014 - “...RilB lmo2104/2105 # iron transport proteins ? RilE comEA/FA, lmo0945 # competence factors ? RliI lmo1035 # phosphotransferase system ? Rli23, 25, 35 lmo0172 # transposases Rli45 rli46 # ? Rli29 lmo9471 # ? Rli30 lmo0506 # ? Only sRNAs are listed for which target genes...”
- “...involved in iron transport, RliE the competence factors comEA/FA, and RliL a phosphotransferase system ( lmo1035 ). The absence of 15 of the 29 sRNAs recently found in L. monocytogenes 72 in the non-pathogenic L. innocua underlines the importance of riboregulators for pathogenesis and virulence. Rli38...”
- Listeria monocytogenes differential transcriptome analysis reveals temperature-dependent Agr regulation and suggests overlaps with other regulons
Garmyn, PloS one 2012 - “...Several genes involved in PTS systems ( lmo0738 , lmo1255 , lmo1973 , lmo1971 , lmo1035 , lmo1901 , lmo2000 , bvrB ) [35] and putative sugar ABC transporters (for example lmo0768 , lmo0767 and lmo0766 ) had higher transcript levels in DG125A compared to EGD-e....”
- Probing the pan-genome of Listeria monocytogenes: new insights into intraspecific niche expansion and genomic diversification
Deng, BMC genomics 2010 - “...166 lmo1032 Similar to transketolase IIIA - 166 lmo1033 Similar to transketolase IIIA - 166 lmo1035 Similar to PTS beta-glucoside-specific enzyme IIABC IIIA + 166 lmo1971 Similar to pentitol PTS system enzyme II C component IIIA + - lmo1972 Similar to pentitol PTS system enzyme II...”
- More
KSF55_15465 PTS sugar transporter subunit IIA from Lactiplantibacillus pentosus
42% identity, 11% coverage
lp_3486 sugar transport protein from Lactobacillus plantarum WCFS1
F9UUF2 PTS regulated carbohydrate transporter, GPH family, raffinose/melibiose/galactose (Can switch between symport (H+) and antiport (Lactose)) from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
42% identity, 11% coverage
- Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum
Lambert, Applied and environmental microbiology 2007 - “...87-104, respectively (Table 2). The third locus, lacS2 (lp_3486) (28), was targeted for mutagenesis in a L. plantarum WCFS1 melA background (NZ5335 [Table 1])....”
- “...resolutions, a small part of the WCFS1 lacS2 gene (lp_3486) (28), which is located directly upstream of melA (lp_3485) and in the same orientation as melA, was...”
- Screening of Lactiplantibacillus plantarum 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying
Chen, Foods (Basel, Switzerland) 2023 - “...the PTS system, N-acetylglucosamine/galactosamine-specific EIIA component (Accession: F9URE0) and Putative PTS system EIIA component (Accession: F9UUF2) in L. plantarum 67 were all enriched in the phosphotransferase system (PTS) and amino sugar and nucleotide sugar metabolism pathways, which underwent significant differential changes in the P/N group (...”
- “...- F9US24 0.18 0.0001 0.25 0.0001 - - F9URE0 0.10 0.0002 0.28 0.0005 2.76 <0.0001 F9UUF2 0.64 0.0033 - - 2.23 <0.0001 P96349 0.58 0.0064 - - 1.65 0.0011 F9UMI2 - - 1.82 0.0015 1.81 0.0016 Q88VM8 0.66 0.0034 - - 1.56 0.0051 F9UPA0 0.34 <0.0001...”
LSEI_0374 Phosphotransferase system IIA component from Lactobacillus casei ATCC 334
LSEI_0374 PTS sugar transporter subunit IIA from Lacticaseibacillus paracasei ATCC 334
40% identity, 46% coverage
PTG3C_BACSU / P20166 PTS system glucose-specific EIICBA component; EII-Glc/EIII-Glc; EIICBA-Glc; EIICBA-Glc 1; EC 2.7.1.199 from Bacillus subtilis (strain 168) (see 2 papers)
TC 4.A.1.1.9 / P20166 The glucose IICBA porter (PtsG) 44% identical to 4.A.1.1.1) from Bacillus subtilis (see 8 papers)
ptsG / GB|CAB13262.1 PTS system glucose-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 from Bacillus subtilis (see 8 papers)
ptsG / CAA77803.1 IIGlc from Bacillus subtilis (see 3 papers)
BSU13890 phosphotransferase system (PTS) glucose-specific enzyme IICBA component from Bacillus subtilis subsp. subtilis str. 168
44% identity, 10% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (By similarity). This system is involved in glucose transport (PubMed:30038046). The system can also transport glucosamine (PubMed:23667565).
function: In addition, plays an important role in the phosphorylation of EIIA-deficient PTS transporters (PubMed:30038046). The EIIA domain can transfer a phosphoryl group to EIIA-deficient PTS transporters, enabling growth with maltose, N-acetylglucosamine, sucrose or trehalose as the sole carbon source (PubMed:30038046).
catalytic activity: N(pros)-phospho-L-histidyl-[protein] + D-glucose(out) = D- glucose 6-phosphate(in) + L-histidyl-[protein] (RHEA:33367)
catalytic activity: D-glucosamine(out) + N(pros)-phospho-L-histidyl-[protein] = D- glucosamine 6-phosphate(in) + L-histidyl-[protein] (RHEA:37359)
disruption phenotype: Deletion of the gene reduces the growth rate on glucose but has no significant effect on growth on glucosamine (PubMed:23667565). The gamP-ptsG double mutant grows slower on glucosamine (PubMed:23667565). Deletion of the gene negatively influences the growth of the cells with maltose, N-acetylglucosamine, sucrose and trehalose (PubMed:30038046). - substrates: glucose
- The Blueprint of a Minimal Cell: MiniBacillus
Reuß, Microbiology and molecular biology reviews : MMBR 2016 - “...BSU25460 BSU25470 BSU25480 BSU28230 Yes No No No No BSU13890 BSU13900 BSU13910 BSU31350 BSU29190 BSU37120 BSU33920 BSU33940 No No No No No No No Yes Yes Yes...”
- Membrane Proteomes and Ion Transporters in Bacillus anthracis and Bacillus subtilis Dormant and Germinating Spores
Chen, Journal of bacteriology 2019 - “...P24138 P24139 P24136 P24137 P38050 Q45064 P39793 P24327 P20166 P46911 P46912 P34957 P36947 P35160 O32047 P16336 P40868 P40869 P31845 Q02115 P42199 O31533 O34348...”
- A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study
Albaum, Proteome science 2011 - “...0.001374 0.000021 2891 O32157 0.000035 0.016583 <0.000001 0.000796 957 O31501 0.000036 0.006965 <0.000001 <0.000001 1681 P20166 0.000046 0.272716 0.044373 0.000003 1948 P21471 0.000052 >0.99 >0.99 0.558509 130 P26906 0.000063 >0.99 >0.99 0.875231 300 P54535 0.000073 >0.99 <0.000001 <0.000001 2391 O31663 0.000143 >0.99 >0.99 0.378199 340 O34633...”
- “...0.007523 <0.000001 1112 O34833 <0.000001 0.007364 <0.000001 0.000001 827 P80877 <0.000001 >0.99 >0.99 0.000002 892 P20166 0.000046 0.272716 0.044373 0.000003 1948 P94565 <0.000001 >0.99 0.264667 0.000003 1033 P19582 <0.000001 >0.99 0.011528 0.000008 903 O31709 <0.000001 >0.99 >0.99 0.000020 295 O32167 0.000020 >0.99 0.001374 0.000021 2891 P25994...”
AO353_04460 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) from Pseudomonas fluorescens FW300-N2E3
52% identity, 6% coverage
- mutant phenotype: Specifically important for NAG utilization.
LPL9_RS10760 PTS beta-glucoside transporter subunit IIBCA from Lacticaseibacillus paracasei
45% identity, 9% coverage
- bglG Regulates the Heterogeneity Driven by the Acid Tolerance Response in Lacticaseibacillus paracasei L9
Shen, Foods (Basel, Switzerland) 2023 - “...gene expression among subpopulations. A total of 17 genes were significantly changed in acid-derived subpopulations. LPL9_RS10760 (PTS beta-glucoside transporter subunit IIBCA), LPL9_RS14735 (BglG family transcriptional antiterminator), and LPL9_RS00305 (ABC transporter permease) were all related to glucoside metabolism and upregulated in the viable subpopulation. These results all...”
- “...(FCM-I/Non-Acid) Annotated Function 1 gene2198 LPL9_RS10370 2.0377 1.0162 Amino acid ABC transporter permease 2 gene2284 LPL9_RS10760 1.6958 1.3129 PTS beta-glucoside transporter subunit IIBCA 3 gene807 LPL9_RS03780 1.275 1.0363 Ironsulfur cluster biosynthesis family protein 4 gene66 LPL9_RS00305 1.2064 1.0832 ABC transporter permease 5 gene3139 LPL9_RS14735 1.0483 1.1387...”
NJ56_RS11145 PTS glucose transporter subunit IIA from Yersinia ruckeri
46% identity, 37% coverage
LEUM_0507 Phosphotransferase system IIA component from Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
41% identity, 43% coverage
CD0816 PTS system, IIABC component from Clostridium difficile 630
50% identity, 8% coverage
SGO_RS09095 sucrose-specific PTS transporter subunit IIBC from Streptococcus gordonii str. Challis substr. CH1
SGO_1857 PTS system, IIABC components from Streptococcus gordonii str. Challis substr. CH1
49% identity, 8% coverage
- Interactions Between Streptococcus gordonii and Fusobacterium nucleatum Altered Bacterial Transcriptional Profiling and Attenuated the Immune Responses of Macrophages
Liu, Frontiers in cellular and infection microbiology 2021 - “...genes in the central cluster include SGO_0281 (SGO_RS01385, PTS glucose transporter subunit IIA, up-regulated), SGO_1857 (SGO_RS09095, PTS beta-glucoside transporter subunit IIBCA, down-regulated), and SGO_1858 (SGO_RS09100, sucrose-6-phosphate hydrolase, down-regulated). These genes encode proteins that participate in the carbohydrate transport process, and they potentially interacted with genes in...”
- Transcriptional profiling of coaggregation interactions between Streptococcus gordonii and Veillonella parvula by Dual RNA-Seq
Mutha, Scientific reports 2019 - “...components EIIB, EIIC and EIID of a mannose, sorbose or fructose transporter, while the other (SGO_RS09095) encodes the EIIBCA components of a sucrose transporter. SGO_RS09095 was also classified under the phosphate-containing compound metabolic process, along with six other genes. Four of these genes were down-regulated between...”
- “...included genes SGO_RS00210/RS00215/RS00220 (old names SGO_00440046), scrK , ldh , lacC (SGO_RS07435, old name SGO_1517), SGO_RS09095 (SGO_1857) and a downstream gene SGO_RS09100 (SGO_1858), encoding a putative sucrose-6-phosphate hydrolase, which was down-regulated approximately 2.5-fold in coaggregates. In addition, SGO_RS02020 (SGO_0405), encoding a putative -n-acetylhexosaminidase, SGO_RS03680 (SGO_0749), encoding...”
- Interactions Between Streptococcus gordonii and Fusobacterium nucleatum Altered Bacterial Transcriptional Profiling and Attenuated the Immune Responses of Macrophages
Liu, Frontiers in cellular and infection microbiology 2021 - “...The genes in the central cluster include SGO_0281 (SGO_RS01385, PTS glucose transporter subunit IIA, up-regulated), SGO_1857 (SGO_RS09095, PTS beta-glucoside transporter subunit IIBCA, down-regulated), and SGO_1858 (SGO_RS09100, sucrose-6-phosphate hydrolase, down-regulated). These genes encode proteins that participate in the carbohydrate transport process, and they potentially interacted with genes...”
- Transcriptional profiling of coaggregation interactions between Streptococcus gordonii and Veillonella parvula by Dual RNA-Seq
Mutha, Scientific reports 2019 - “...genes SGO_RS00210/RS00215/RS00220 (old names SGO_00440046), scrK , ldh , lacC (SGO_RS07435, old name SGO_1517), SGO_RS09095 (SGO_1857) and a downstream gene SGO_RS09100 (SGO_1858), encoding a putative sucrose-6-phosphate hydrolase, which was down-regulated approximately 2.5-fold in coaggregates. In addition, SGO_RS02020 (SGO_0405), encoding a putative -n-acetylhexosaminidase, SGO_RS03680 (SGO_0749), encoding glutathione-disulfide...”
- Distinct Regulatory Role of Carbon Catabolite Protein A (CcpA) in Oral Streptococcal spxB Expression
Redanz, Journal of bacteriology 2018 - “...IIC components, glucose/maltose/N-acetylglucosamine specific (SSA_0456, SGO_1857); pykF, pyruvate kinase gene (SSA_0848; SGO_1339); manL, gene for...”
- Physiological and molecular characterization of genetic competence in Streptococcus sanguinis
Rodriguez, Molecular oral microbiology 2011 - “...SSA_1972 SGO_1731 SP_2001 SSA_1973 SGO_1732 SP_2002 SSA_1974 SGO_1733 SP_0338 SSA_2096 clpL SGO_1856 SP_1722 SSA_0456 scrA SGO_1857 --- SSA_0860 SGO_2013 --- --- SGO_2086 --- --- SGO_2088 SP_0054 purK SSA_0040 purK SGO_2089 purK SP_0051 purD SSA_0037 purD SGO_2092 purD --- --- SGO_2093 --- SSA_0036 * SGO_2094 --- ---...”
BC_5320 PTS sugar transporter subunit IIA from Bacillus cereus ATCC 14579
BC5320, NP_834982 PTS system, glucose-specific IIA component from Bacillus cereus ATCC 14579
41% identity, 42% coverage
- Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579
Laouami, PloS one 2014 - “...a decrease of the abundance of the IIA component of the glucose-specific phosphotransferase (PTS) system (BC_5320, log 2 =0.4; p -value=0.021). A significant decrease in abundance was also observed for the pyruvate-formate-lyase-activating enzyme, PflA (BC_0492; log 2 =1; p -value=0.048). PflA is the sole enzyme able...”
- Methionine Residues in Exoproteins and Their Recycling by Methionine Sulfoxide Reductase AB Serve as an Antioxidant Strategy in Bacillus cereus
Madeira, Frontiers in microbiology 2017 - “...Log 2 fold-change EE LE S Carbohydrate metabolism NA BC5137 Tpi Triosephosphate isomerase 1.50 NP_834982 BC5320 Ccr PTS system, glucose-specific IIA component 1.82 NP_834343 BC4637 Ack acetate kinase 1.09 NP_832706 BC2959 Mqo Malate:quinone oxidoreductase 2.82 NP_831487 BC1712 FumB Fumarate hydratase 2.57 NP_833692 BC3973 PdhA Pyruvate dehydrogenase...”
- “...description Log 2 fold-change EE LE S Carbohydrate metabolism NA BC5137 Tpi Triosephosphate isomerase 1.50 NP_834982 BC5320 Ccr PTS system, glucose-specific IIA component 1.82 NP_834343 BC4637 Ack acetate kinase 1.09 NP_832706 BC2959 Mqo Malate:quinone oxidoreductase 2.82 NP_831487 BC1712 FumB Fumarate hydratase 2.57 NP_833692 BC3973 PdhA Pyruvate...”
- Low concentrations of bile salts induce stress responses and reduce motility in Bacillus cereus ATCC 14579 [corrected]
Kristoffersen, Journal of bacteriology 2007 - “...BC4602 Metabolism; glycolysis, fermentation, and electron transport BC5320 BC0668 BC4599 BC4600 BC4962 BC3721 BC1376 Protein synthesis BC0135 BC5075 BC0154...”
- “...dhg; f, BC4599 pyruvate kinase; }, BC1376 flavodoxin; OE, BC5320 PTS, glucose specific. VOL. 189, 2007 TRANSCRIPTIONAL RESPONSE TO BILE SALTS IN B. CEREUS 5309...”
BTF1_24980 PTS sugar transporter subunit IIA from Bacillus thuringiensis HD-789
41% identity, 42% coverage
- NagRBt Is a Pleiotropic and Dual Transcriptional Regulator in Bacillus thuringiensis
Cao, Frontiers in microbiology 2018 - “...that NagR Bt could activate these genes, rather than repress them. Glucose-specific phosphotransferase enzyme IIA (BTF1_24980), phosphoenolpyruvate-protein phosphotransferase (BTF1_18555), phosphocarrier protein HPr (BTF1_18560), and the PTS lactose cellobiose family IIC subunit (BTF1_24425) involved in the phosphotransferase system showed enhanced expression as previously reported in S. coelicolor...”
- “...7.428 NT BTF1_24425 chbC PTS system cellobiose-specific transporter subunit IIC / / / 4.807 NT BTF1_24980 crr PTS system glucose-specific transporter subunit IIA 251 acttgtctagaggtct 8.87 75.026 23.846 1.973 BTF1_25170 XRE family transcriptional regulator / / / 33.600 NT BTF1_25685 lrgB Antiholin-like protein LrgB / /...”
lp_2969 N-acetylglucosamine PTS, EIICBA from Lactobacillus plantarum WCFS1
46% identity, 9% coverage
RUO99_14415 glycoside-pentoside-hexuronide (GPH):cation symporter from Lactiplantibacillus plantarum
42% identity, 11% coverage
- Analysis of the Probiotic Potential of Lactiplantibacillus plantarum LB1_P46 Isolated from the Mexican Fermented Pulque Beverage: A Functional and Genomic Analysis
Giles-Gómez, Microorganisms 2024 - “...indicated these two proteins as glycoside-pentoside-hexuronide (GPH): cation symporters (locus_tag RUO99_14330, protein_id WNW15682.1 and locus_tag RUO99_14415, protein_id WNW15699.1, complement 3,044,336..3,046,294, respectively). Protein-BLAST analysis identified a lactose permease with an identity of 99.69% and 99.54% of L. plantarum , respectively. Although it is reported that L. plantarum...”
CRIB_2018 PTS sugar transporter subunit IIA from Romboutsia ilealis
41% identity, 45% coverage
EfmE1162_1485 PTS transporter subunit IIBC from Enterococcus faecium E1162
40% identity, 10% coverage
- A LacI-family regulator activates maltodextrin metabolism of Enterococcus faecium
Zhang, PloS one 2013 - “...homology search in E1162 for the orthologs of this gene cluster identified a gene cluster (EfmE1162_1485 - EfmE1162_1489; here named malRMBPT ) which is predicted to encode five proteins with amino acid identities ranging from 59% to 87% to their homologs in E. faecalis V583 (...”
- “...malP was greatly induced in M1+maltose compared to BHI. These qPCR data indicate that the EfmE1162_1485 - EfmE1162_1489 gene cluster is regulated independently of MdxR and may be involved in maltose utilization in E. faecium , similar to its homologous gene cluster in E. faecalis [...”
YPO2995 PTS system, glucose-specific IIA component from Yersinia pestis CO92
y1485 PTS system, glucose-specific IIA component from Yersinia pestis KIM
YPTB2717 PTS system, glucose-specific IIA component, permease from Yersinia pseudotuberculosis IP 32953
44% identity, 37% coverage
- High-throughput analysis of Yersinia pseudotuberculosis gene essentiality in optimised in vitro conditions, and implications for the speciation of Yersinia pestis
Willcocks, BMC microbiology 2018 - “...with intestinal mucosal surfaces [ 62 ], Y. pestis has a glucose-specific PTS component ( ypo2995 ), appropriate for a pathogen adapted to replicating in the blood, where glucose is the dominant sugar available. These differences may also be explained by the fact that glucose uptake...”
- Growth of Yersinia pseudotuberculosis in human plasma: impacts on virulence and metabolic gene expression
Rosso, BMC microbiology 2008 - “...YPTB2716 YPO2994 ptsI putative PTS sytem, enzyme I component 1.946 (< 0.001) 1.351 (0.038) YPTB2717 YPO2995 crr putative PTS system, glucose-specific IIA component, permease 1.611 (0.002) 1.440 (0.117) Sugar metabolism YPTB0074 YPO0078 pfkA putative 6-phosphofructokinase 1.594 (0.017) 1.430 (0.073) YPTB3195 YPO0920 fbaA, fba, fda possible fructose-bisphosphate...”
- “...1.697 (0.001) YPTB2716 (ptsI) YPO2994 PTS sytem. enzyme I component 1.945 (< 0.001) YPTB2717 (crr) YPO2995 PTS system. glucose-specific IIA component 1.611 (0.002) 0.731 (0.028) YPTB2962 YPO2644 conserved hypothetical protein (pseudogene. IS100) 2.68 (< 0.001) YPTB3078 YPO0834 putative PTS transport protein 1.742 (0.025) YPTB3190 (rpiA) YPO0915...”
- Adaptive response of Yersinia pestis to extracellular effectors of innate immunity during bubonic plague
Sebbane, Proceedings of the National Academy of Sciences of the United States of America 2006 - “...relative to that of the reference gene crr (YPO2995, encoding a phosphotransferase system component), whose expression level was not affected by in vivo or...”
- Deletion of Yersinia pestis ail Causes Temperature-Sensitive Pleiotropic Effects, Including Cell Lysis, That Are Suppressed by Carbon Source, Cations, or Loss of Phospholipase A Activity
Kolodziejek, Journal of bacteriology 2021 - “...metabolism, carbon: TCA cycle 1 3.5 y1507 Putative aminotransferase Amino acid biosynthesis: alanine 1 3.9 y1485 crr Glucose specific PTS system component Transport of small molecules; carbohydrates, organic acids, alcohols 1 8.3 y1027 clpP ATP dependent Clp protease proteolytic subunit Degradation of proteins, peptides 1 9.2...”
- Differential regulation of the hmsCDE operon in Yersinia pestis and Yersinia pseudotuberculosis by the Rcs phosphorelay system
Guo, Scientific reports 2015 - “...The quantity of mRNA was normalized relative to the quantity of the reference gene crr (y1485) 40 . The ratio of the normalized quantity of hmsD mRNA in different strains to the normalized quantity in the wild-type samples was calculated. Primers and probe sets used are...”
- A LysR-Type Transcriptional Regulator, RovM, Senses Nutritional Cues Suggesting that It Is Involved in Metabolic Adaptation of Yersinia pestis to the Flea Gut
Vadyvaloo, PloS one 2015 - “...for each experimental gene was normalized relative to the quantity of the reference gene crr (y1485), whose expression is not affected by in vivo or in vitro growth conditions [ 1 , 25 , 26 ]. The S1 Dataset contains raw data from the RT-qPCR using...”
- Role of the PhoP-PhoQ gene regulatory system in adaptation of Yersinia pestis to environmental stress in the flea digestive tract
Vadyvaloo, Microbiology (Reading, England) 2015 - “...(Life Technologies) as per the manufacturer's instructions. Samples were amplified using oligonucleotide primers for crr (y1485), y1667, y0666, y3909, y3519, y2882 ( psaA ), y1918 and the IQ SYBR Green Supermix (Bio-Rad) via a two-step protocol on a Bio-Rad CFX384 real-time system. Oligonucleotide sequences are tabulated...”
- The Yersinia pestis Rcs phosphorelay inhibits biofilm formation by repressing transcription of the diguanylate cyclase gene hmsT
Sun, Journal of bacteriology 2012 - “...relative to the quantity of the reference gene crr (y1485), whose expression level was not affected by in vivo or in vitro growth conditions (29). The ratio...”
- Differential control of Yersinia pestis biofilm formation in vitro and in the flea vector by two c-di-GMP diguanylate cyclases
Sun, PloS one 2011 - “...for each experimental gene was normalized relative to the quantity of the reference gene crr (y1485), whose expression level is not affected by in vivo or in vitro growth conditions [45] . The ratio of the normalized mRNA quantity of y3730 and hmsT to crr was...”
- Transit through the flea vector induces a pretransmission innate immunity resistance phenotype in Yersinia pestis
Vadyvaloo, PLoS pathogens 2010 - “...for each experimental gene was normalized relative to the quantity of the reference gene crr (y1485), and the ratio of the normalized quantity of each gene in the flea samples to the normalized quantity in the in vitro samples was calculated ( Fig. S2 ). Primer...”
- “...The quantity of each mRNA was determined relative to that of the reference gene crr (y1485). Fold-differences in transcript levels of the 12 Y. pestis genes in the flea compared to (A) in vitro biofilm, (B) exponential phase planktonic cultures, and (C) stationary phase planktonic cultures...”
- IscR is essential for yersinia pseudotuberculosis type III secretion and virulence
Miller, PLoS pathogens 2014 - “...PTS system, glucose-specific IIBC component ptsG 2.0 YPTB2682 ABC transporter, periplasmic iron(III)-binding protein sfuA 2.6 YPTB2717 PTS system glucose-specific IIA component, permease crr 2.3 YPTB2770 probable ABC transporter, ATP-binding subunit 2.8 YPTB2771 putative ABC iron transporter 2.7 YPTB2772 ABC transporter, periplasmmic iron binding protein 4.1 YPTB3957...”
- Growth of Yersinia pseudotuberculosis in human plasma: impacts on virulence and metabolic gene expression
Rosso, BMC microbiology 2008 - “...(0.109) YPTB2716 YPO2994 ptsI putative PTS sytem, enzyme I component 1.946 (< 0.001) 1.351 (0.038) YPTB2717 YPO2995 crr putative PTS system, glucose-specific IIA component, permease 1.611 (0.002) 1.440 (0.117) Sugar metabolism YPTB0074 YPO0078 pfkA putative 6-phosphofructokinase 1.594 (0.017) 1.430 (0.073) YPTB3195 YPO0920 fbaA, fba, fda possible...”
- “...phosphocarrier protein 1.697 (0.001) YPTB2716 (ptsI) YPO2994 PTS sytem. enzyme I component 1.945 (< 0.001) YPTB2717 (crr) YPO2995 PTS system. glucose-specific IIA component 1.611 (0.002) 0.731 (0.028) YPTB2962 YPO2644 conserved hypothetical protein (pseudogene. IS100) 2.68 (< 0.001) YPTB3078 YPO0834 putative PTS transport protein 1.742 (0.025) YPTB3190...”
SSA_0456 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, putative from Streptococcus sanguinis SK36
42% identity, 11% coverage
lp_3513 beta-glucosides PTS, EIIBCA from Lactobacillus plantarum WCFS1
F9UUH8 PTS system, beta-glucosides-specific EIIBCA component from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
36% identity, 12% coverage
lmo1017 similar to phosphotransferase system glucose-specific enzyme IIA from Listeria monocytogenes EGD-e
52% identity, 34% coverage
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...sugar transporter subunit IIA lmo1002 ptsH Phosphocarrier protein HPr lmo1003 lmo1003 Phosphotransferase system enzyme I lmo1017 1.24 Yes/down lmo1017 PTS glucose transporter subunit IIA lmo1035 lmo1035 PTS beta-glucoside transporter subunit IIABC lmo1095 3.29 Yes/down lmo1095 PTS cellbiose transporter subunit IIB lmo1255 1.52 Yes/up 4.14 Yes/down lmo1255...”
- Cross Talk among Transporters of the Phosphoenolpyruvate-Dependent Phosphotransferase System in Bacillus subtilis
Morabbi, Journal of bacteriology 2018 - “...monocytogenes 1255 [ lmo1255 ]) is assumed to be phosphorylated by EIIA Glc encoded by lmo1017 ( 42 ). The GlcNAc and trehalose transporters also share the EIIA Glc subunit in Vibrio cholerae ( 43 ). In Borrelia burgdorferi , EIIA Glc encoded by the crr...”
- Development of a mariner-based transposon and identification of Listeria monocytogenes determinants, including the peptidyl-prolyl isomerase PrsA2, that contribute to its hemolytic phenotype
Zemansky, Journal of bacteriology 2009 - “...lmo0734 lmo0785 lmo0813 lmo0821 lmo0833 lmo0964 lmo0964, lmo1017 lmo1079 lmo1254 lmo1255 lmo1268 lmo1329 lmo1354 lmo1401 lmo1475 lmo1514, lmo1515 lmo1575...”
- Glycerol metabolism and PrfA activity in Listeria monocytogenes
Joseph, Journal of bacteriology 2008 - “...lmo0912a lmo0945 lmo0947 lmo0960 lmo0981 gbuA gbuB gbuCa,b lmo1017 lmo1073 pheS pheT proA proB glnR glnA lmo1300 smbA lmo1424 lmo1431 zurM udk lmo1516b lmo1517b...”
LMOf2365_1038 PTS system, glucose-specific, IIA component, putative from Listeria monocytogenes str. 4b F2365
52% identity, 34% coverage
LM6179_1334 PTS sugar transporter subunit IIA from Listeria monocytogenes 6179
LMOf6854_1066 PTS system, glucose-specific, IIA component, putative from Listeria monocytogenes str. 1/2a F6854
52% identity, 34% coverage
A5I073 PTS system, beta-glucoside-specific IIabc component from Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
CBO0881 PTS system, beta-glucoside-specific IIabc component from Clostridium botulinum A str. ATCC 3502
47% identity, 10% coverage
- Identification of potential drug targets and vaccine candidates in Clostridium botulinum using subtractive genomics approach
Sudha, Bioinformation 2019 - “...179 Isochorismatase A5I0D8 Yes - 10 CBO0881 cbo02060 629 PTS system beta-glucoside-specific transporter subunit IIABC A5I073 Yes COG1264[G] 11 CBO2715 cbo00521, cbo00523 177 dTDP-4-dehydrorhamnose 3,5-epimerase A5I5E9 Yes COG1898[M] 12 CBO1432 cbo01503 283 N-acetylmuramoyl-L-alanine amidase A5I1R2 Yes COG0860[M] 13 CBO3111 cbo01503 967 N-acetylmuramoyl-L-alanine amidase A5I6J2 Yes COG0860[M]...”
- Identification of potential drug targets and vaccine candidates in Clostridium botulinum using subtractive genomics approach
Sudha, Bioinformation 2019 - “...14 shortlisted targets were found to be interacting with different C. botulinum strains and protein CBO0881 has maximum 50 interactors. Target with higher interactors was considered as metabolically important active protein, and hence could be used as good drug target [ 31 ]. Functionality analysis: We...”
- “...ABC transporter permease A5I0Z1 No COG0765[E] 9 CBO0945 - 179 Isochorismatase A5I0D8 Yes - 10 CBO0881 cbo02060 629 PTS system beta-glucoside-specific transporter subunit IIABC A5I073 Yes COG1264[G] 11 CBO2715 cbo00521, cbo00523 177 dTDP-4-dehydrorhamnose 3,5-epimerase A5I5E9 Yes COG1898[M] 12 CBO1432 cbo01503 283 N-acetylmuramoyl-L-alanine amidase A5I1R2 Yes COG0860[M]...”
- Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clusters
Raphael, Applied and environmental microbiology 2008 - “...the ha/orfX A1 strains consisting of CBO0640, CBO0652, CBO0881, CBO1050, CBO2468, CBO2615, and CBO3197 also failed to produce amplification products using the...”
CD630_04690 PTS beta-glucoside transporter subunit IIBCA from Clostridioides difficile 630
CD0469 PTS system, IIabc component from Clostridium difficile 630
41% identity, 11% coverage
- Clostridioides difficile strain-dependent and strain-independent adaptations to a microaerobic environment
Weiss, Microbial genomics 2021 - “...striking differences in transcription of sugar transporters. Four gene clusters involved in sucrose transport ( CD630_04690 ), mannose transport ( CD630_2567025680 ), lactose/cellobiose transport ( CD630_3443034450 ) and xylose utilization ( xylA , xynA , xynB ) exhibited higher expression in strain 630 than CD196. C....”
- The repertoire of ABC proteins in Clostridioides difficile
Pipatthana, Computational and structural biotechnology journal 2021 - “...ABC conserved motif and are not associated with transport activity [2] . Eleven proteins (cd630_03880, cd630_04690, cd630_08160, cd630_22690, cd630_30750, cd630_30860, cd630_30970, cd630_31160, cd630_31250, cd630_31340, and cd630_31370) annotated as phosphoryl transfer protein subunit IIABC were also omitted from further analyses. Although the phosphotransferase system (PTS) is involved...”
- Pleiotropic role of the RNA chaperone protein Hfq in the human pathogen Clostridium difficile
Boudry, Journal of bacteriology 2014 - “...(CD630_n00170), an sRNA located in the IGR between CD0469 Journal of Bacteriology Downloaded from http://jb.asm.org/ on February 11, 2017 by University of...”
- Genome-wide identification of regulatory RNAs in the human pathogen Clostridium difficile
Soutourina, PLoS genetics 2013 - “...Figure 5 shows two examples of growth phase-regulated sRNAs: RCd6 located in the IGR between CD0469 and CD0470 genes encoding a saccharose-type IIABC component of the PTS system and a beta-lactamase-inducing penicillin-binding protein (BlaR), respectively, and SQ1498 located in IGR between CD2112 and CD2113 genes encoding...”
SSU05_1490 Phosphotransferase system IIC component, glucose/maltose/N-acetylglucosamine-specific from Streptococcus suis 05ZYH33
SSU98_1501 Phosphotransferase system IIC component, glucose/maltose/N-acetylglucosamine-specific from Streptococcus suis 98HAH33
SSU1309 beta-glucoside-specific phosphotransferase system (PTS), IIABC component from Streptococcus suis P1/7
54% identity, 8% coverage
- The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis
Zhang, Frontiers in cellular and infection microbiology 2017 - “...involved in various PTSs were down-regulated, including manX/Y/Z (SSU05_17781780)encoding mannose transporter, ptsG/crr/bglF (SSU05_0397, SSU05_0398, and SSU05_1490) encoding maltose/glucose transporter, celC (SSU05_0709) encoding cellobiose transporter, and ulaA/ulaB (SSU05_2062, and SSU05_2063) encoding ascorbate transporter. Down-regulation of these pathways and related genes were potentially consistent with the decreased bacterial...”
- In vitro transcriptome analysis of two Chinese isolates of Streptococcus suis serotype 2
Zhang, Genomics, proteomics & bioinformatics 2014 - “...mannose/fructose/ N -acetylgalactosamine-specific component IID ManZ SSU05_0452 2.33 PTS, mannose/fructose/ N -acetylgalactosamine-specific component IID BglF SSU05_1490 5.40 PTS IIC component, glucose/maltose/ N -acetylglucosamine-specific Strain EC ID Gene ID FC Protein Glycolysis/gluconeogenesis pathway 98HAH33 2.7.1.69 SSU98_0384 1.92 PTS IIC component, glucose 3.2.1.86 SSU98_2083 1.02 Alpha-galactosidase 1.1.1.1 SSU98_0274...”
- In vitro transcriptome analysis of two Chinese isolates of Streptococcus suis serotype 2
Zhang, Genomics, proteomics & bioinformatics 2014 - “...PTS cellobiose-specific component IIC AgaE SSU98_1232 1.71 PTS, mannose ManZ SSU98_0440 1.80 PTS, mannose BglF SSU98_1501 6.74 PTS IIC component, glucose 05ZYH33 LacF SSU05_1038 1.11 PTS cellobiose-specific component IIA Pts / Crr SSU05_0398 1.56 PTS IIC component, glucose/maltose/ N -acetylglucosamine-specific AgaW SSU05_1218 2.59 PTS, mannose/fructose/ N...”
- Precision Genome Engineering in Streptococcus suis Based on a Broad-Host-Range Vector and CRISPR-Cas9 Technology
Gussak, ACS synthetic biology 2023 - “...SSU0154 Pgk 1.28 SSU0312 Fba 1.56 SSU0483 Tpi 1.12 SSU0927 Ldh 1.34 SSU1308 bglH 1.41 SSU1309 1.54 SSU1320 Eno 1.46 SSU1451 gpmA 1.47 SSU1836 Pgi 1.07 pyrimidine metabolism SSU0735 pyrR 2.55 SSU0736 pyrB 2.45 SSU0737 carA 2.54 SSU0738 carB 2.64 SSU0860 sntC 1.23 SSU0861 pyrC 2.41...”
- FlpS, the FNR-Like Protein of Streptococcus suis Is an Essential, Oxygen-Sensing Activator of the Arginine Deiminase System
Willenborg, Pathogens (Basel, Switzerland) 2016 - “...by our microarray analysis. SSU0357 and SSU1583 presumably encode for glucose-specific PTS whereas SSU0217 and SSU1309 encode for trehalose- and -glucoside PTS, respectively. Glucose and glucose-containing di- and oligosaccharides are the major energy sources of S. suis. Since expression of gapA and gpmA was slightly affected...”
HI1711 PTS system, glucose-specific IIA component (crr) from Haemophilus influenzae Rd KW20
43% identity, 38% coverage
- Position-based scanning for comparative genomics and identification of genetic islands in Haemophilus influenzae type b
Bergman, Infection and immunity 2003 - “...Eagan genome between the two ORFs designated HI1710 and HI1711 in Rd. This segment is flanked by direct repeats of the 7-base sequence 5-GTAAGTA and contains an...”
- “...Rd genome) 2 4 Between HI1261 and HI1262 Between HI1710 and HI1711 Within HI1266 5 Within HI1403 3 6 7 8 a Insert size (bp) % GC contenta Flanking repeat 600...”
- Effect of multiple mutations in the hemoglobin- and hemoglobin-haptoglobin-binding proteins, HgpA, HgpB, and HgpC, of Haemophilus influenzae type b
Morton, Infection and immunity 1999 - “...HI689 HI1704 HI1705 HI1706 HI1707 HI1708 HI1709 HI1710 HI1711 HI1712 HI1713 HI1714 HI1715 HI1716 HI1717 Reference or source VOL. 67, 1999 H. INFLUENZAE...”
- “...three hgp genes. Lanes: A, H. influenzae hgpA mutant HI1711 chromosomal DNA EcoRI digest; B, H. influenzae hgpB mutant HI1712 chromosomal DNA EcoRI digest; C,...”
- Role of the two-component signal transduction and the phosphoenolpyruvate: carbohydrate phosphotransferase systems in competence development of Haemophilus influenzae Rd
Gwinn, Journal of bacteriology 1996 - “...Wild type crr ptsI crr ptsI sxy-1 crr sxy-1 ptsI sxy-1 a HI1711 HI1712 HI0601 MIV assay Transient anaerobiosis Early log phase 1 2 1 2 1 2 100 119 57 82 100 68...”
USA300HOU_0276 PTS family glucose/glucoside (glc) porter component IIABC from Staphylococcus aureus subsp. aureus USA300_TCH1516
SAUSA300_0259 PTS system, IIA component from Staphylococcus aureus subsp. aureus USA300_FPR3757
46% identity, 20% coverage
- Pre-epidemic evolution of the MRSA USA300 clade and a molecular key for classification
Bianco, Frontiers in cellular and infection microbiology 2023 - “..., intergenic (2736965) , intergenic (2773434) , hisB (2836831) intergenic (14363), USA300HOU_0113 (115190), gatC1 (291025), USA300HOU_0276 (311262), lplA1 (381083), USA300HOU_0470 (495436), USA300HOU_0498 (542064), tagX (714370), USA300HOU_0721 (771214), USA300HOU_072 1 (771429), intergenic (810108), intergenic (856156), mnhD2 (944473), oppA1 (993238), menF (1047527), pdhB (1104230), intergenic (1199330), USA300HOU_1599 (1706793),...”
- Impact of the pentose phosphate pathway on metabolism and pathogenesis of Staphylococcus aureus
Kim, PLoS pathogens 2023 (no snippet) - Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Genetic Factors Involved in Human Endothelial Cells Damage, an Important Phenotype Correlated with Persistent Endovascular Infection
Xiao, Antibiotics (Basel, Switzerland) 2022 - “...protein 17.88 16.40 SAUSA300_1032 hypothetical putative iron compound ABC transporter iron compound-binding protein 17.87 9.01 SAUSA300_0259 hypothetical PTS system, IIA component 17.72 4.08 SAUSA300_1070 hypothetical hypothetical protein 17.66 6.61 SAUSA300_1474 hypothetical hypothetical protein 17.57 3.84 SAUSA300_1451 hypothetical short chain dehydrogenase/reductase family oxidoreductase 17.47 4.46 SAUSA300_0769 hypothetical...”
- Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection
Vitko, mBio 2016 - “...predicted to encode glucose transporters: SAUSA300_0191 ( glcA ), SAUSA300_0194, SAUSA300_0236 ( glcC ), and SAUSA300_0259 (see TableS1 ). Importantly, glucose is (i) largely absent from the skin surface, (ii) the most abundant free carbohydrate in human serum, and (iii) used by activated innate immune cells...”
ETAE_1130 glucose-specific PTS system component from Edwardsiella tarda EIB202
46% identity, 37% coverage
Halsa_0150 PTS sugar transporter subunit IIA from Halanaerobium hydrogeniformans
42% identity, 38% coverage
PFLU5027 putative multiphosphoryl transfer protein from Pseudomonas fluorescens SBW25
46% identity, 6% coverage
EHLA_0193 PTS transporter subunit IIBC from Anaerobutyricum hallii
43% identity, 9% coverage
- Unravelling lactate-acetate and sugar conversion into butyrate by intestinal Anaerobutyricum and Anaerostipes species by comparative proteogenomics
Shetty, Environmental microbiology 2020 - “...higher abundance in cells grown on sucrose (Table S1 ). We identified two locus tags EHLA_0193 and EHLA_0331 encoding a proteinNpiphosphohistidinesugar PTS system. The protein encoded by EHLA_0331 was less abundant (log 2 FC >4, adjusted p value = 0.039) in sorbitol compared to sucrose. Also...”
- “...hallii DSM3353. The products encoded by the A. soehngenii genes with locus tags EHLA_0192 and EHLA_0193 were not identified in A. hallii , while a homologue of the protein encoded by the gene with locus tag EHLA_0195 was identified, which shared 52% identity with a 4alphaglucanotransferase...”
D9QA35 PTS transporter subunit EIIC from Corynebacterium pseudotuberculosis (strain C231)
CpC231_0932 glucose PTS transporter subunit IIA from Corynebacterium pseudotuberculosis C231
48% identity, 9% coverage
- Changes in protein abundance are observed in bacterial isolates from a natural host
Rees, Frontiers in cellular and infection microbiology 2015 - “...and metabolism D9QA16 gpsA CpC231_0913 Glycerol-3-phosphate dehydrogenase 0.005 1.388 * 0.405 Energy production and conversion D9QA35 ptsG CpC231_0932 Phosphotransferase system II Component 0.800 * 0.370 0.481 Carbohydrate transport and metabolism D9QAE1 fhs CpC231_1040 Formatetetrahydrofolate ligase 1.007 * 0.355 0.293 Nucleotide transport and metabolism D9QAL4 ribD CpC231_1114...”
- Changes in protein abundance are observed in bacterial isolates from a natural host
Rees, Frontiers in cellular and infection microbiology 2015 - “...D9QA16 gpsA CpC231_0913 Glycerol-3-phosphate dehydrogenase 0.005 1.388 * 0.405 Energy production and conversion D9QA35 ptsG CpC231_0932 Phosphotransferase system II Component 0.800 * 0.370 0.481 Carbohydrate transport and metabolism D9QAE1 fhs CpC231_1040 Formatetetrahydrofolate ligase 1.007 * 0.355 0.293 Nucleotide transport and metabolism D9QAL4 ribD CpC231_1114 Riboflavin biosynthesis...”
Q5A_018245 PTS glucose transporter subunit IIA from Serratia inhibens PRI-2C
44% identity, 37% coverage
SERP_RS10495 glucose-specific PTS transporter subunit IIBC from Staphylococcus epidermidis RP62A
Q5HL73 PTS system glucose-specific EIICBA component from Staphylococcus epidermidis (strain ATCC 35984 / DSM 28319 / BCRC 17069 / CCUG 31568 / BM 3577 / RP62A)
SERP2114 PTS system, IIABC components from Staphylococcus epidermidis RP62A
42% identity, 11% coverage
- The Vancomycin Resistance-Associated Regulatory System VraSR Modulates Biofilm Formation of Staphylococcus epidermidis in an ica-Dependent Manner
Wu, mSphere 2021 - “...formation of citrate from acetyl-CoA and oxaloacetate 0.45 ND Genes involved in phosphate transport system SERP_RS10495 NC_002976.3 (21376272139654) PTS glucose EIICBA component 0.14 0.480.01 SERP_RS11510 NC_002976.3 (23896042389978) PTS mannose transporter subunit IIA 0.41 ND SERP_RS11515 NC_002976.3 (23899822390557) Dihydroxyacetone kinase subunit L 0.46 ND SERP_RS11135 NC_002976.3 (22894202291360)...”
- Phenotypic Modulation of Biofilm Formation in a Staphylococcus epidermidis Orthopedic Clinical Isolate Grown Under Different Mechanical Stimuli: Contribution From a Combined Proteomic Study
Bottagisio, Frontiers in microbiology 2020 - “...Triose-phosphate isomerase (0.00083) TPIS_STAEQ Q5HQV2 Triose-phosphate isomerase (0.00138) TPIS_STAEQ Q5HQV2 Triose-phosphate isomerase partial (0.00917) PTG3C_STAEQ Q5HL73 PTS system glucose-specific EIICBA component (0.00535) ALF1_STAEQ Q5HL21 Fructose-bisphosphate aldolase class 1 (0.00231) LDH_STAEQ Q5HL31 L-lactate dehydrogenase (0.0032) MDH_STAEQ Q5HR46 Malate dehydrogenase (0.000989) Q5HNL0_STAEQ Q5HNL0 Citrate synthase (0.0016) Q5HQ26_STAEQ Q5HQ26...”
- Skin-to-blood pH shift triggers metabolome and proteome global remodelling in Staphylococcus epidermidis
Gonçalves, Frontiers in microbiology 2022 - “...Phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) Fructose-specific IIABC components SERP2260 Sucrose-specific IIBC components SERP1900/68 Glucose-specific EIICBA component SERP2114 ptsG 6-Phospho-beta-galactosidase SERP1789 lacG Tagatose 1,6-diphosphate aldolase SERP1792 lacD Membrane Transporters: Hemin, Iron and Mn Multidrug resistance efflux pump SERP1767 SepA Flotillin-like protein SERP1140 FloA RND family efflux transporter SERP0014...”
- The Vancomycin Resistance-Associated Regulatory System VraSR Modulates Biofilm Formation of Staphylococcus epidermidis in an ica-Dependent Manner
Wu, mSphere 2021 - “...genes manA2 (mannose-6-phosphate isomerase), malA (alpha-glucosidase), rbsKD (ribokinase), etc.; and phosphate transport-related genes such as serp2114 (phosphotransferase [PTS] glucose EIICBA component), serp2343 (PTS mannose transporter subunit IIA), serp2260 (fructose-specific IIABC components), and lacF (PTS lactose-specific phosphotransferase IIA component) ( Table2 ). TABLE2 Transcription levels of genes...”
- “...serp2324 , serp2325 , sucCD , serp0731 , serp0732 ), the phosphate transport system ( serp2114 , serp2343 , serp2344 , lacFD ), and the carbohydrate metabolic process ( rbsKDU , gluD , manA2 ) were downregulated in the vraSR mutant strain in comparison with parent...”
- A New Bioactive Compound From the Marine Sponge-Derived Streptomyces sp. SBT348 Inhibits Staphylococcal Growth and Biofilm Formation
Balasubramanian, Frontiers in microbiology 2018 - “...sdhA L -serine dehydratase iron-sulfur-dependent alpha subunit -2.1059 3.48E-44 SERP2112 Alcohol dehydrogenase zinc-containing -2.1470 2.46E-14 SERP2114 PTS system IIABC components -3.3405 5.78E-34 budA Alpha-acetolactate decarboxylase -2.4764 2.38E-37 budB Acetolactate synthase catabolic -3.4875 2.65E-55 ldh L -lactate dehydrogenase -3.3363 1.49E-48 SERP2345 Dihydroxyacetone kinase family protein -2.0835 3.63E-14...”
RDJ18_RS01200 glucose PTS transporter subunit IIA from Staphylococcus aureus
SAR0263 putative PTS transport system protein from Staphylococcus aureus subsp. aureus MRSA252
44% identity, 20% coverage
- Multiomics analysis of Staphylococcus aureus ST239 strains resistant to virulent Herelleviridae phages
Kornienko, Scientific reports 2024 - “...of glucose and fructose into the cell at both early and late stages (RDJ18_RS00835, RDJ18_RS01075, RDJ18_RS01200, RDJ18_RS03920, RDJ18_RS13495, RDJ18_RS14335) (Table S4 ). The key genes encoding enzymes involved in glycolysis exhibited upregulation: pyruvate kinase (RDJ18_RS06570), L-lactate dehydrogenase (RDJ18_RS01065), phosphoglycerate kinase (RDJ18_RS04310), triose-phosphate isomerase (RDJ18_RS04315), and 2,3-bisphosphoglycerate-independent...”
- The Staphylococcus aureus response to unsaturated long chain free fatty acids: survival mechanisms and virulence implications
Kenny, PloS one 2009 - “...SAR0235 putative PTS system, IIBC component 2.22 1.30E-03 SAR0242 putative galactitol PTS component 2.13 1.76E-02 SAR0263 putative PTS system protein 2.50 3.44E-02 SAR0355 Cys/Met metabolism PLP-dependent enzyme 2.04 2.74E-02 SAR0522 putative pyridoxal 5-phosphate biosynthesis protein 3.03 3.00E-04 SAR0523 SNO glutamine amidotransferase family protein 2.70 3.70E-04 SAR0752...”
- “...namely SAR0235 , SAR1777 ( pfkA ), SAR2720 , SAR2721 ( pmi ), SAR0242 and SAR0263 were also down-regulated. This could indicate an alteration of central metabolism via the action of the linoleic acid. Here, in concert with these changes, many genes in glycolysis were up-regulated,...”
LP_RS00755, lp_0185 sucrose-specific PTS transporter subunit IIBC from Lactiplantibacillus plantarum WCFS1
lp_0185 sucrose PTS, EIIBCA from Lactobacillus plantarum WCFS1
45% identity, 8% coverage
- Saccharomyces cerevisiae strains L7 contribute to flavor and deacidification in Suanyu, a Chinese traditional fermented fish
Wenkang, NPJ science of food 2024 - “...amino sugar and nucleotide sugar metabolism. In the starch and sucrose metabolism pathway, the genes LP_RS00755 (coding for scrA, sucrose phosphotransferase), LP_RS00760 (coding for sacA, fructofuranosidase), and LP_RS00750 (coding for scrK, fructokinase) were downregulated. Multiple genes associated with sugar metabolism were downregulated under co-culture conditions, indicating...”
- Strain diversity of plant-associated Lactiplantibacillus plantarum
Yu, Microbial biotechnology 2021 - “...to a limited extent on sucrose (Fig. 2 ), lacks the first 650bp of pts1BCA (lp_0185), a gene in the sucrose phosphoenolpyruvate (PEP)dependent phosphotransferase system (PTS; Saulnier etal ., 2007 ; Yin etal ., 2018 ). The number of gene clusters in the other COG categories...”
- Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study
Petrov, Genes 2021 - “...glucose transporter subunit IIA NC 2.30 lp_0575, pts9AB PTS mannose transporter subunit IIAB NC 6.36 lp_0185, pts1BCA PTS -glucoside transporter subunit IIBCA NC 4.96 lp_2649, pts19C PTS N-acetylgalactosamine transporter IIC 12.45 2.88 lp_3009, pts23B PTS sugar transporter subunit IIB 2.19 3.02 lp_0265, pts5ABC PTS transporter subunit...”
- Lifestyle of Lactobacillus plantarum in the mouse caecum
Marco, Environmental microbiology 2009 - “...al., 2004 ). Seven of these genes, including (i) pts 19A (lp_2647) and pts 1BCA (lp_0185) encoding components of N -acetylgalactosamine and sucrose PTS systems, (ii) dhaT , encoding 1,3 propanediol dehydrogenase, and (iii) adhE , encoding a bifunctional alcohol dehydrogenase and acetaldehyde dehydrogenase, were also...”
- Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays
Saulnier, Applied and environmental microbiology 2007 - “...the products of the gene encoding a sucrose PTS (lp_0185) in Lactobacillus spp. strains other than L. plantarum strains were low. The first proteins showing a...”
- “...M FOD1.5b Energy metabolism and transport lp_0184 lp_0185 lp_0187 lp_0189 lp_0193 lp_0280 lp_0286 lp_0329 lp_0393 lp_0764 lp_0849 lp_1101 lp_1102 lp_1105...”
- An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence
Sturme, Journal of bacteriology 2005 - “...and trehalose phosphotransferase system genes lp_0185, lp_0436, and lp_0264, respectively). Cluster 2 encompassed constitutively down-regulated genes,...”
- “...University of California, Berkeley Cluster 1 lp_0184 lp_0185 lp_0187 lp_0188 lp_0189 lp_0263 lp_0264 lp_0435 lp_0436 lp_3480 lp_3481 lp_3482 Geneb L. PLANTARUM...”
P43470 PTS system sucrose-specific EIIBCA component from Pediococcus pentosaceus
45% identity, 8% coverage
OG1RF_11317 sucrose-specific PTS transporter subunit IIBC from Enterococcus faecalis OG1RF
37% identity, 12% coverage
U876_06285 PTS glucose transporter subunit IIA from Aeromonas hydrophila NJ-35
44% identity, 37% coverage
VP0793 PTS system, glucose-specific IIA component from Vibrio parahaemolyticus RIMD 2210633
44% identity, 37% coverage
- The Gene Cluster Cj0423-Cj0425 Negatively Regulates Biofilm Formation in Campylobacter jejuni
Wang, International journal of molecular sciences 2024 - “...uncharacterized functional gene vp0610 in Vibrio parahaemolyticus , identifying 180 interacting proteins, including Hfq, VP0710, VP0793, and CyaA, all of which are known to play a role in biofilm formation. This demonstrates the efficacy of the method, as evidenced by the identification of 93 proteins using...”
- A Novel Gene vp0610 Negatively Regulates Biofilm Formation in Vibrio parahaemolyticus
Jiang, Frontiers in microbiology 2021 - “...Pull-down assays revealed that VP0610 can interact with 180 proteins, some of which (Hfq, VP0710, VP0793, and CyaA) participate in biofilm formation. Moreover, deleting vp0610 enhanced the expression of genes responsible for biofilm component ( flaE ), the sugar phosphotransferase system (PTS) EIIA component ( vp0710...”
- “...). Specifically, RNA binding protein (Hfq), adenylate cyclase (CyaA), and PTS-related protein components (VP0710 and VP0793) were identified as key proteins involved in biofilm formation via the quorum sensing and cAMP/CRP pathways ( Supplementary Figure 1 ). Therefore, we will focus on cAMP/CRP pathways and quorum...”
- QseC Inhibition as a Novel Antivirulence Strategy for the Prevention of Acute Hepatopancreatic Necrosis Disease (AHPND)-Causing Vibrio parahaemolyticus
Yang, Frontiers in cellular and infection microbiology 2020 - “...enzyme CpsD 8 VP0791 -2.52929 5.65E-11 Flagellin protein FlaE VP0792 -3.17949 3.71E-24 Flagellin protein FlaD VP0793 -2.61879 1.70E-19 Flagellin protein FlaC VP0794 -2.43402 5.55E-16 Flagellar hook-associated protein FlgL VP0795 -2.2913 1.66E-14 Flagellar hook-associated protein FlgK VP0796 -2.39126 1.93E-13 Flagellar protein FlgJ [peptidoglycan hydrolase] VP0797 -2.70585 7.15E-18...”
- Marker for type VI secretion system effectors
Salomon, Proceedings of the National Academy of Sciences of the United States of America 2014 - “...Of these 13 proteins, seven (VP0035, VP0070, VP0762, VP0793, VP2731, VP2767, and VPA1376) are also encoded by V. parahaemolyticus AQ3810, a strain that does...”
LLKF_0663 PTS system sucrose-specific transporter subunit IIABC from Lactococcus lactis subsp. lactis KF147
40% identity, 11% coverage
- Strain-Dependent Transcriptome Signatures for Robustness in Lactococcus lactis
Dijkstra, PloS one 2016 - “...DnaK negative 12.8 LLKF_1695 thiL acetyl-CoA acetyltransferase positive 4.6 LLKF_0551 dfpA phosphopantothenoylcysteine decarboxylase positive 1.5 LLKF_0663 scrA PTS system sucrose-specific transporter subunit IIABC positive 0.5 LLKF_2232 hypothetical protein negative 0.9 LLKF_1965 sufD SUF system FeS cluster assembly protein SufD positive 11.3 LLKF_0510 adaA methylphosphotriester-DNA alkyltransferase positive...”
AO356_17540 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) from Pseudomonas fluorescens FW300-N2C3
50% identity, 6% coverage
- mutant phenotype: Specific phenotype on NAG as a carbon source. Not sure why SEED think s this includes the IIA component.
M7W_1488 PTS glucose transporter subunit IIA from Enterococcus faecium ATCC 8459 = NRRL B-2354
44% identity, 36% coverage
- <i>In vitro</i> resensitization of multidrug-resistant clinical isolates of <i>Enterococcus faecium</i> and <i>E. faecalis</i> through phage-antibiotic synergy
Ghatbale, Antimicrobial agents and chemotherapy 2025 - “...downregulated genes, which included heat shock protein Hsp20 (M7W_2017), an N-acetylglucosamine-specific IIA, IIB, IIC component (M7W_1488), a cadmium-translocating P-type ATPase (M7W_1465), a manganese transport protein MntH (M7W_1001), a universal stress protein family (M7W_1773, uspA ), a multiple sugar ABC transporter, an ATP-binding protein (M7W_2275, msmK_1 ),...”
SA0255 hypothetical protein from Staphylococcus aureus subsp. aureus N315
44% identity, 20% coverage
BglS / b3722 β-glucoside specific PTS enzyme II / BglG kinase / BglG phosphatase (EC 2.7.1.199) from Escherichia coli K-12 substr. MG1655 (see paper)
bglF / P08722 β-glucoside specific PTS enzyme II / BglG kinase / BglG phosphatase (EC 2.7.1.199) from Escherichia coli (strain K12) (see 59 papers)
TC 4.A.1.2.2 / P08722 β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF from Escherichia coli (see 6 papers)
NP_418178 beta-glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase from Escherichia coli str. K-12 substr. MG1655
b3722 fused beta-glucoside-specific PTS enzymes: IIA component/IIB component/IIC component from Escherichia coli str. K-12 substr. MG1655
48% identity, 10% coverage
- substrates: beta-glucosides
tcdb comment: The bgl operon, encoding BglF, is regulated by antitermination when the RNA antiterminator protein, BglG, binds to one of two RAT sites in the mRNA (Gordon et al. 2015) - Spatial arrangement of the beta-glucoside transporter from Escherichia coli.
Yagur-Kroll, Journal of bacteriology 2009 - GeneRIF: The results demonstrate that the big cytoplasmic loop participates in creating the sugar pathway and explain the observed coupling between translocation of phosphotransferase system sugars from the periplasm to the cytoplasm and their phosphorylation.
- Genetic dissection of the divergent activities of the multifunctional membrane sensor BglF.
Monderer-Rothkoff, Journal of bacteriology 2007 - GeneRIF: This study identified distinct sites in BglF that are involved in regulating phosphate flow via the common active-site residue in response to environmental cues.
- Dynamic membrane topology of the Escherichia coli beta-glucoside transporter BglF.
Yagur-Kroll, The Journal of biological chemistry 2005 (PubMed)- GeneRIF: analysis of the Escherichia coli beta-glucoside transporter BglF membrane topology
- Genetic dissection of the divergent activities of the multifunctional membrane sensor BglF
Monderer-Rothkoff, Journal of bacteriology 2007 (secret) - The bvr locus of Listeria monocytogenes mediates virulence gene repression by beta-glucosides
Brehm, Journal of bacteriology 1999 - “...PTS permease components (accession numbers: ArbF, P26207; BglF, P08722; and BglP, P40739; identities with BvrB: 37, 34, and 32%, respectively). The positions of...”
- Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones
Lee, Applied and environmental microbiology 2009 - “...b3311 b3426 b3447 b3453 b1488 b3540 b3541 b3542 b3588 b3704 b3722 b4051 b4067 b4069 b4119 b4149 b4187 b4227 b1197 b4239 b4240 5.3 6.1 8.0 4.0 8.6 12.1 8.0 13.9...”
- Protein disorder is positively correlated with gene expression in Escherichia coli
Paliy, Journal of proteome research 2008 - “...(b3255); L) RpoZ (b3649); M) AtpC (b3731); N) AtpD (b3722); O) AtpF (b3736); P) NusG (b3982); Q) GroES (b4142); R) GroEL (b4143). J Proteome Res. Author...”
- Differential gene expression for investigation of Escherichia coli biofilm inhibition by plant extract ursolic acid
Ren, Applied and environmental microbiology 2005 - “...1.6 0.0074 1.4 0.0170 1.5 0.0004 b1312 bglF fhuD b1312 b3722 b0152 1.0 1.0000 1.4 0.0546 1.1 0.8560 1.6 0.0319 1.6 0.0053 1.6 0.0032 nikE yhaO yhjX b3480 b3110...”
NWMN_0199 PTS system, IIA component from Staphylococcus aureus subsp. aureus str. Newman
SAOUHSC_00235 hypothetical protein from Staphylococcus aureus subsp. aureus NCTC 8325
44% identity, 20% coverage
lp_0264 glucose PTS transporter subunit IIA from Lactiplantibacillus plantarum WCFS1
F9UT61 PTS system, trehalose-specific IIBC component from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
lp_0264 beta-glucosides PTS, EIIABC from Lactobacillus plantarum WCFS1
46% identity, 9% coverage
- Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study
Petrov, Genes 2021 - “...8-1. We observed the strongest downregulation among the PTS transporters in two C-subunits: lp_0265 and lp_0264. They were 14.69- and 25.45-fold downregulated in 8-1 and 18.57- and 38.68-fold in Ym1, respectively. Based on a 73.2% match with the respective proteins in Listeria monocytogenes , they seem...”
- “...PTS sugar transporter subunit IIB 2.19 3.02 lp_0265, pts5ABC PTS transporter subunit EIIC 18.57 14.69 lp_0264, pts4ABC PTS transporter subunit EIIC 38.68 25.45 lp_0170, dak3 PTS-dependent dihydroxyacetone kinase phosphotransferase subunit DhaM 4.86 164.91 * The difference in gene expression was estimated as the fold change (FC)...”
- Characterization of transcriptional response of Lactobacillus plantarum under acidic conditions provides insight into bacterial adaptation in fermentative environments.
Jung, Scientific reports 2020 - “...hydrolase lp_0263 F9UT60 COG0366 K01226 pts4ABC 1.38 2.08E08 1.88E07 PTS system, trehalose-specific IIBC component lp_0264 F9UT61 COG2190 K02757 lp_0302 3.20 2.46E64 2.09E62 Extracellular transglycosylase lp_0302 F9UTQ7 NOG102536 K01185 lp_0304 1.60 5.83E32 1.85E30 Extracellular transglycosylase lp_0304 F9UTQ8 NOG62861 K01185 lp_0783 2.61 1.48E116 3.01E114 Oligopeptide ABC transporter lp_0783...”
- Identification of key proteins and pathways in cadmium tolerance of Lactobacillus plantarum strains by proteomic analysis.
Zhai, Scientific reports 2017 - “...2.73 Q88RZ2 rbsD; D-ribose mutarotase 1.78 Q88S51 rhaA; L-rhamnose isomerase 1.56 PTS system PTS system F9UT61 pts4ABC; PTS system trehalose-specific transporter subunit IIBC 1.86 F9UL45 pts9AB; PTS system, mannose-specific EIIAB component 1.73 F9UL47 pts9D; PTS system, mannose-specific EIID component 1.82 F9UL56 pts10A; PTS system, mannose-specific EIIA...”
- Characterization of transcriptional response of Lactobacillus plantarum under acidic conditions provides insight into bacterial adaptation in fermentative environments
Jung, Scientific reports 2020 - “...Trehalose-6-phosphate hydrolase lp_0263 F9UT60 COG0366 K01226 pts4ABC 1.38 2.08E08 1.88E07 PTS system, trehalose-specific IIBC component lp_0264 F9UT61 COG2190 K02757 lp_0302 3.20 2.46E64 2.09E62 Extracellular transglycosylase lp_0302 F9UTQ7 NOG102536 K01185 lp_0304 1.60 5.83E32 1.85E30 Extracellular transglycosylase lp_0304 F9UTQ8 NOG62861 K01185 lp_0783 2.61 1.48E116 3.01E114 Oligopeptide ABC transporter...”
- Transcriptional Reprogramming at Genome-Scale of Lactobacillus plantarum WCFS1 in Response to Olive Oil Challenge
Esteban-Torres, Frontiers in microbiology 2017 - “...These genes included those within the trehalose operon [ lp_0262 (transcriptional repressor)], lp_0263 (trehalose-6-phosphate hydrolase), lp_0264 and lp_0265 (PTS system trehalose-specific transporter subunits IIBC), the GABA transporter ( lp_1722 ) and two aquaporins ( lp_0372 and lp_3436 ), which were all downregulated. This profile indicates that...”
- An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence
Sturme, Journal of bacteriology 2005 - “...phosphotransferase system genes lp_0185, lp_0436, and lp_0264, respectively). Cluster 2 encompassed constitutively down-regulated genes, with the highest effect...”
- “...1 lp_0184 lp_0185 lp_0187 lp_0188 lp_0189 lp_0263 lp_0264 lp_0435 lp_0436 lp_3480 lp_3481 lp_3482 Geneb L. PLANTARUM agr-LIKE TWO-COMPONENT REGULATORY SYSTEM...”
NJ74_RS04255 PTS beta-glucoside transporter subunit IIABC from Escherichia coli DH5[alpha]
48% identity, 10% coverage
Clocel_2778 glucose-specific PTS transporter subunit IIBC from Clostridium cellulovorans 743B
39% identity, 9% coverage
- Clostridium cellulovorans Proteomic Responses to Butanol Stress
Costa, Frontiers in microbiology 2021 - “...phosphorylase activity ( Fieulaine et al., 2001 ). In addition, a putative glucose PTS transporter (Clocel_2778) is up-regulated in butanol-challenged C. cellulovorans . The latter result seems consistent with increased specific glucose consumption observed in cells grown in butanol-supplemented conditions. However, three glycolytic enzymes, namely phosphoglycerate...”
- “...cellulovorans . This observation possibly correlates with the up-regulation of a putative glucose PTS transporter (Clocel_2778). In addition, pyruvate fate was significantly perturbed by butanol-stress in C. cellulovorans . Formate production was inhibited (consistent with down-regulation of PFL-activating enzyme, Clocel_1812), and a higher proportion of the...”
Q46072 protein-Npi-phosphohistidine-D-mannose phosphotransferase (EC 2.7.1.191); protein-Npi-phosphohistidine-D-glucose phosphotransferase (EC 2.7.1.199) from Corynebacterium glutamicum (see 2 papers)
ptsM / AAA53546.1 phosphoenolpyruvate sugar phosphotransferase from Corynebacterium glutamicum (see 2 papers)
NCgl1305 glucose PTS transporter subunit IIA from Corynebacterium glutamicum ATCC 13032
cg1537 glucose-specific enzyme II BC component of PTS from Corynebacterium glutamicum ATCC 13032
45% identity, 9% coverage
- Transcriptome analysis of Corynebacterium glutamicum in the process of recombinant protein expression in bioreactors
Sun, PloS one 2017 - “...kinase, which is a critical gene in the PTS system, was up-regulated by 2.98-fold, and NCgl1305 and NCgl2553 encoding the phosphotransferase system IIC component were up-regulated by 4.54 and 3.86-fold, respectively, both consistently showing the up absorption and transfer of glucose. Increased glucose consumption during the...”
- The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum
Gaigalat, BMC molecular biology 2007 - “...enzyme IIABC cg2121 NCgl1862 ptsH 89 9.1 Phosphocarrier protein HPr, PTS component non clustered cg1537 NCgl1305 ptsG 683 72.6 Glucose-specific PTS component, enzyme IIBCA cg2925 NCgl2553 ptsS 661 69.1 Sucrose-specific PTS component, enzyme IIBCA cg3365 NCgl2933 - 513 52.7 L-ascorbate type PTS component, enzyme IIC cg3366...”
- “...adhA 3.23 13.00 Alcohol dehydrogenase cg2118 NCgl1859 - 3.00 12.00 Transcriptional regulator protein, DeoR-family cg1537 NCgl1305 ptsG 2.70 11.97 Phosphotransferase system (PTS), glucose-specific enzyme IIBCA component cg2119 NCgl1860 fruK 2.67 11.59 1-Phosphofructokinase cg2025 - - 2.62 11.43 Hypothetical protein cg2034 NCgl1739 - 2.38 11.24 Hypothetical protein...”
- Adaptation of Corynebacterium glutamicum to ammonium limitation: a global analysis using transcriptome and proteome techniques
Silberbach, Applied and environmental microbiology 2005 - “...related NCgl0074 NCgl0893 NCgl0894 NCgl0895 NCgl0896 NCgl0897 NCgl1305 NCgl1521 NCgl1875 NCgl1876 NCgl1877 NCgl1878 NCgl1915 NCgl1917 NCgl1918 Acc. no. 2394...”
- CRISPRi-Library-Guided Target Identification for Engineering Carotenoid Production by Corynebacterium glutamicum
Göttl, Microorganisms 2021 - “...plasmid carrying the pta (cg3048) sgRNA This work pS_dCas9_ ptsG pS_dCas9 plasmid carrying the ptsG (cg1537) sgRNA This work pS_dCas9_ pyc pS_dCas9 plasmid carrying the pyc (cg0791) sgRNA This work pS_dCas9_ pyk pS_dCas9 plasmid carrying the pyk (cg2291) sgRNA This work pS_dCas9_ ramB pS_dCas9 plasmid carrying...”
- HrrSA orchestrates a systemic response to heme and determines prioritization of terminal cytochrome oxidase expression
Keppel, Nucleic acids research 2020 - “...Glucose uptake cg2121 ptsH Phosphocarrier protein HPr, general component of PTS 70 2.1 1.2 0.3 cg1537 ptsG Glucose-specific EIIABC component EIIGlc of PTS 70 1.6 1.1 0.1 cg2091 ppgG Polyphosphate glucokinase 199 266 0.2 0.8 cg0223 iolT1 Myo-Inositol transporter 1, alternative glucose uptake system 73 2.0...”
- Identifying the Growth Modulon of Corynebacterium glutamicum
Haas, Frontiers in microbiology 2019 - “...to that of the growth rate. The same trend was true for the genes, ptsG (cg1537) and ptsI (cg2117), encoding the glucose uptake system as well as the gene encoding iolT1 (cg0223) which has been shown to also enable glucose uptake. Genes ppgk (cg2091) and cg2932...”
- Substrate-dependent cluster density dynamics of Corynebacterium glutamicum phosphotransferase system permeases
Martins, Molecular microbiology 2019 - “...C. glutamicum PTS for fructose, glucose and sucrose are shown in Fig. 1 . ptsG (cg1537) encodes the glucosespecific membrane integral EIIBCA transporter, while ptsS (cg2925) encodes the sucrosespecific EIIBCA, and ptsF (cg2120) the fructosespecific EIIABC (Fig. 1 ). A fourth putative PTS belongs to the...”
- Impact of CO2/HCO3- Availability on Anaplerotic Flux in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains
Krüger, Journal of bacteriology 2019 (secret) - Phosphotransferase system-mediated glucose uptake is repressed in phosphoglucoisomerase-deficient Corynebacterium glutamicum strains
Lindner, Applied and environmental microbiology 2013 - “...of C. glutamicum WT Inactivation of ptsG (cg1537) in C. glutamicum pgi L-Lysine-producing strain (pycP458S, homV59A, lysCT311I) In-frame deletion of pgi...”
- “...Derived from pBB1, for constitutive expression of ptsG (cg1537) of C. glutamicum Specr; C. glutamicum/E. coli shuttle vector (Ptac lacIq; pBL1, OriVC.g.,...”
- Maltose uptake by the novel ABC transport system MusEFGK2I causes increased expression of ptsG in Corynebacterium glutamicum
Henrich, Journal of bacteriology 2013 - “...of pDrive in cg2701 In-frame deletion of ptsG gene (cg1537) of C. glutamicum WT American Type Culture Collection 41 42 27 This work This work This work...”
- “...cg2708 to cg2703 (mus genes) and of the ptsG (cg1537) gene were constructed using pK19mobsacB as described TABLE 2 Plasmids used in this study Plasmid Relevant...”
- Protein turnover quantification in a multilabeling approach: from data calculation to evaluation
Trötschel, Molecular & cellular proteomics : MCP 2012 - “...LAAGEVVDIVSPLEGK.A (charge 2) from the protein PtsG (Cg1537), a component of the phosphotransferase system. Calculated incorporation rates (ape) of the partly...”
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TC 4.A.3.2.5 / Q8L3C4 IIA aka Crr, component of The N,N' -diacetylchitobiose Enzyme II (Toratani et al., 2008) (>80% identical to the E. coli enzyme (4.A.3.2.1)) from Serratia marcescens (see paper)
43% identity, 37% coverage
- substrates: N,N' -diacetylchitobiose
tcdb comment: The IIA protein is the Serratia glucose IIA which is nearly identical to the E. coli IIAGlc (Crr)
NWMN_RS14005 glucose-specific PTS transporter subunit IIBC from Staphylococcus aureus subsp. aureus str. Newman
SAOUHSC_02848 PTS system glucose-specific IIABC component from Staphylococcus aureus subsp. aureus NCTC 8325
SAUSA300_2476 phosphotransferase system, glucose-specific IIABC component from Staphylococcus aureus subsp. aureus USA300_FPR3757
SACOL2552 PTS system, IIABC components from Staphylococcus aureus subsp. aureus COL
40% identity, 10% coverage
- Citrate serves as a signal molecule to modulate carbon metabolism and iron homeostasis in Staphylococcus aureus
Chen, PLoS pathogens 2024 - “...genes encoding virulence factors (i.e., hla and scn ), phosphotransferase system (PTS) (i.e., NWMN_RS01850-NWMN_RS01835 operon, NWMN_RS14005 ), aa3 -type terminal oxidase ( qoxABCD operon), and transcriptional regulators (i.e., cstR , farR and sarR ) ( S2C Fig and S2 Table ). In contrast, transcription of clpC...”
- Phenotypic Plasticity of Staphylococcus aureus in Liquid Medium Containing Vancomycin
Rong, Frontiers in microbiology 2019 - “...2588313 SAOUHSC_02809 gntR A/G P < - > P 41.46% Gluconate operon transcriptional repressor 2621187 SAOUHSC_02848 glcB C/T A < - > A 43.90% PTS system glucose-specific transporter subunit IIABC Position 264897 is located in SAOUHSC_00246, which is related to drug transporters. GuaA (SNP 382501) encodes...”
- Lysogenization of Staphylococcus aureus RN450 by phages ϕ11 and ϕ80α leads to the activation of the SigB regulon
Fernández, Scientific reports 2018 - “...6.49 Up SAOUHSC_02812 3.00 7.98 Up SAOUHSC_02815 2.63 4.38 Up SAOUHSC_02822 fbp 2.31 3.17 Up SAOUHSC_02848 glcB 2.07 2.90 SAOUHSC_02862 clpL 5.24 16.79 Up SAOUHSC_02877 crtN 2.14 5.93 Up SAOUHSC_02879 crtM 3.46 10.17 Up SAOUHSC_02880 crtQ 3.88 13.11 Up SAOUHSC_02881* crtP 3.08 10.10 Up SAOUHSC_02882 crtO...”
- Impact of the pentose phosphate pathway on metabolism and pathogenesis of Staphylococcus aureus
Kim, PLoS pathogens 2023 (no snippet) - A Membrane-Bound Transcription Factor is Proteolytically Regulated by the AAA+ Protease FtsH in Staphylococcus aureus
Yeo, Journal of bacteriology 2020 (secret) - Glucose Augments Killing Efficiency of Daptomycin Challenged Staphylococcus aureus Persisters
Prax, PloS one 2016 - “...first two chosen strains exhibited disrupted phosphotransferase (PTS) systems, namely the glucose-PTS specific IIABC component, SAUSA300_2476 (NE39) and the glucose-PTS specific IIBC component domain protein, SAUSA300_0191 (NE172). Compared to our PTS-positive control strain NE1046 ( fdhD , formate dehydrogenase, SAUSA300_2231), both PTS mutants killing curves showed...”
- Transcriptomic and Metabolomic Analysis of a Fusidic Acid-Selected fusA Mutant of Staphylococcus aureus
Gupta, Antibiotics (Basel, Switzerland) 2022 - “...in SH10001st-2 ( Tables S1 and S2 ). With regard to carbohydrate transporters, 3 (SACOL2146, SACOL2552, and treP ) were downregulated and another 2 (SAV0192 and ptaA ) were upregulated ( Tables S1 and S2 ). The downregulation of SH10001st-2 genes directly involved in pyruvate metabolism...”
- Virulence and Metabolism
Richardson, Microbiology spectrum 2019 (secret) - Changes in the Staphylococcus aureus transcriptome during early adaptation to the lung
Chaffin, PloS one 2012 - “...transport (10%) were upregulated in vivo. Transcription of the primary glucose PTS uptake system, ptsG (SACOL2552) was increased in fresh LB 4.9 fold compared to stationary phase, but increased by 7.3, 9.8 and 16.6 fold after 30, 120 and 6 hours in the lung, respectively. Also...”
- A defect in menadione biosynthesis induces global changes in gene expression in Staphylococcus aureus
Kohler, Journal of bacteriology 2008 - “...and fructose (SACOL2663) and also of glucose (ptsI, SACOL0175, SACOL2552) (see Table S2 in the supplemental material). Very recently, it was shown that the menD...”
NO343_03030 PTS sugar transporter subunit IIA from Mycoplasma capricolum subsp. capricolum
52% identity, 34% coverage
SAR2618 PTS system, glucose-specific IIABC component from Staphylococcus aureus subsp. aureus MRSA252
40% identity, 10% coverage
KQ76_13275 glucose-specific PTS transporter subunit IIBC from Staphylococcus aureus
40% identity, 10% coverage
RDJ18_RS13495 glucose-specific PTS transporter subunit IIBC from Staphylococcus aureus
40% identity, 10% coverage
- Multiomics analysis of Staphylococcus aureus ST239 strains resistant to virulent Herelleviridae phages
Kornienko, Scientific reports 2024 - “...and fructose into the cell at both early and late stages (RDJ18_RS00835, RDJ18_RS01075, RDJ18_RS01200, RDJ18_RS03920, RDJ18_RS13495, RDJ18_RS14335) (Table S4 ). The key genes encoding enzymes involved in glycolysis exhibited upregulation: pyruvate kinase (RDJ18_RS06570), L-lactate dehydrogenase (RDJ18_RS01065), phosphoglycerate kinase (RDJ18_RS04310), triose-phosphate isomerase (RDJ18_RS04315), and 2,3-bisphosphoglycerate-independent phosphoglycerate mutase...”
PTS3B_STRMU / P12655 PTS system sucrose-specific EIIBCA component; EIIBCA-Scr; EII-Scr; EC 2.7.1.211 from Streptococcus mutans serotype c (strain ATCC 700610 / UA159) (see 2 papers)
P12655 protein-Npi-phosphohistidine-sucrose phosphotransferase (EC 2.7.1.211) from Streptococcus mutans serotype c (see 3 papers)
scrA / GB|AAN59464.1 PTS system, sucrose-specific, EIIBCA component; EC 2.7.1.69 from Streptococcus mutans (see paper)
SMUGS5_08275, SMU_1841, SMU_RS08435 sucrose-specific PTS transporter subunit IIBC from Streptococcus mutans UA159
40% identity, 11% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (PubMed:2536656, PubMed:468378). This system is involved in sucrose transport (PubMed:2536656, PubMed:468378).
catalytic activity: N(pros)-phospho-L-histidyl-[protein](out) + sucrose = sucrose 6(G)-phosphate(in) + L-histidyl-[protein] (RHEA:49236) - Identification of the antibacterial action mechanism of diterpenoids through transcriptome profiling
Moon, Frontiers in microbiology 2022 - “...* AA CA KA DM SMU_RS09355 PTS transporter subunit EIIC ptsG 1.39 3.48 2.10 2.37 SMU_RS08435 PTS beta-glucoside transporter subunit IIBCA scrA 0.68 3.83 2.06 2.13 SMU_RS04505 PTS transporter subunit EIIC bglP 0.62 4.11 2.20 2.14 SMU_RS06770 PTS transporter subunit EIIC lacE 0.65 1.42 1.45 1.69...”
- Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability
Naka, Frontiers in cellular and infection microbiology 2022 - “...SMU_1905c Bacteriocin secretion protein 1029103 SMU_1905c 2.748 scrA PTS system sucrose-specific transporter subunit IIABC 1029057 SMU_1841 2.701 SMU_1118c ABC sugar transporter, permease 1028430 SMU_1118c 2.609 opuBc Choline ABC transporter, osmoprotectant binding protein 1028413 SMU_1095 2.592 trk Potassium uptake protein TrkA 1028798 SMU_1562 2.544 pacL Cation-transporting P-type...”
- D‑Tagatose inhibits the growth and biofilm formation of Streptococcus mutans
Hasibul, Molecular medicine reports 2018 - “...EII-mal/glu-F acacaatatctttggcggtaaga 105 EII-mal/glu-R cgaaaccagagataataccaacg SMUGS5_03875 Fructose-specific PTS subunit IIABC EII-fru-F tgatacttttaaggctgggatca 116 EII-fru-R aaaagaaccgttgcttcctttac SMUGS5_08275 Sucrose-specific PTS subunit IIABC EII-sucF ggtgtctttggtgctctgtattc 113 EII-suc-R gtcaccatgaccagtaccatttt SMUGS5_R09892 16S ribosomal RNA 16SF cctacgggaggcagcagtag 101 16SR caacagagctttacgatccgaaa...”
- RNA-Seq Reveals Enhanced Sugar Metabolism in Streptococcus mutans Co-cultured with Candida albicans within Mixed-Species Biofilms
He, Frontiers in microbiology 2017 - “...Ajdi and Pham, 2007 ; Moye et al., 2014 ). Here, we observed that scrA (SMU_1841), scrB (SMU_1843), and scrK (SMU_1840) were significantly up-regulated in dual-species biofilms (vs. single species S. mutans ; Supplementary Table S1 ). The scr A gene encodes a high-affinity sucrose PTS...”
- Systematic analysis of lysine malonylation in Streptococcus mutans
Li, Frontiers in cellular and infection microbiology 2022 - “...name Protein description Modified sites Cytoplasmic membrane I6L922 SMU_765 NADH oxidase/alkyl hydroperoxidase reductase peroxide-forming 109 P12655 scrA PTS system sucrose-specific EIIBCA component 46, 601 Q8CVC6 prsA Foldase protein PrsA 253 Q8DRU8 opuCa Putative osmoprotectant amino acid ABC transporter, ATP-binding protein 23 Q8DRV8 SMU_2104 APC family permease(predicted)...”
- Shotgun Metagenomics of a Water Kefir Fermentation Ecosystem Reveals a Novel Oenococcus Species
Verce, Frontiers in microbiology 2019 - “...PTS EIIBCA components encoded by Pediococcus pentosaceus and Streptococcus mutans (Swissprot accession numbers P43470 and P12655 , respectively) was found only in L. harbinensis, L. hordei/mali , and L. nagelii , all in the vicinity of a -fructofuranosidase/sucrose-6-phosphate hydrolase-encoding gene. Genes encoding the mannose-class PTS components...”
PBPRA0861 putative PTS system, glucose-specific IIAcomponent from Photobacterium profundum SS9
43% identity, 37% coverage
KSF55_00925 sucrose-specific PTS transporter subunit IIBC from Lactiplantibacillus pentosus
45% identity, 8% coverage
- Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3
Raethong, Heliyon 2025 - “...KSF55_15895 Galactitol PTS Down Up KSF55_15890 Galactitol PTS Down Up KSF55_15680 Ascorbate PTS Down Up KSF55_00925 Sucrose PTS Down Up a The up- or down-regulated DEGs in the glucose culture were considered by comparing glucose to sucrose or lactose cultures. b The up- or down-regulated DEGs...”
- “...in starch and sucrose metabolism were more enriched in the sucrose culture, such as KSF55_00920, KSF55_00925, KSF55_00930, and KSF55_00940, which encode fructokinase, sucrose PTS system, beta-fructofuranosidase, and oligo-1,6-glucosidase, respectively. 3.4 Carbon source-mediated transcriptional change patterns in L. pentosus 9D3 A hierarchical clustering analysis compared the gene...”
lmo2787 beta-glucoside-specific phosphotransferase enzyme II ABC component from Listeria monocytogenes EGD-e
38% identity, 12% coverage
- Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...lmo2782 PTS cellbiose transporter subunit IIB lmo2783 3.66 Yes/down lmo2783 PTS cellbiose transporter subunit IIC lmo2787 4.02 Yes/down bvrB Beta-glucoside-specific phosphotransferase enzyme II ABC component lmo2797 2.71 Yes/down lmo2797 PTS mannitol transporter subunit IIA lmo2799 2.55 Yes/down lmo2799 PTS mannitol transporter subunit IIBC a "" means...”
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - New Insights into the Lactic Acid Resistance Determinants of Listeria monocytogenes Based on Transposon Sequencing and Transcriptome Sequencing Analyses
Liu, Microbiology spectrum 2023 - “...beta-glucoside transporter subunit IIABC 0.0834 1.54E-13 lmo2782 lmo2782 PTS cellobiose transporter subunit IIB 0.1655 4.48E-02 lmo2787 bvrB beta-Glucoside-specific phosphotransferase enzyme II ABC component 0.2198 3.85E-04 lmo2797 lmo2797 PTS mannitol transporter subunit IIA 0.0452 8.74E-29 lmo2799 lmo2799 PTS mannitol transporter subunit IIBC 0.0043 5.16E-23 a P adj,...”
- Pyruvate carboxylase plays a crucial role in carbon metabolism of extra- and intracellularly replicating Listeria monocytogenes
Schär, Journal of bacteriology 2010 - “...lmo2742d lmo2743 lmo2771b lmo2772b lmo2773b lmo2786 (bvrC) lmo2787 (bvrB)b lmo2788 (bvrA)b lmo2797b lmo2798b lmo2851 Encoded protein 1780 SCHAR ET AL. J....”
- Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes
Hain, Journal of bacteriology 2006 - “...of -glucoside (lmo0738, lmo0874lmo0876, lmo1035, and lmo2787), fructose (lmo2733 and lmo0503), galactiol (lmo0507 and lmo0508), pentitol (lmo1971lmo1973),...”
- Genome diversification in phylogenetic lineages I and II of Listeria monocytogenes: identification of segments unique to lineage II populations
Zhang, Journal of bacteriology 2003 - “...(LPXTG) Lmo2603 (unknown) Lmo2604 (unknown) Lmo2786 (BvrC) Lmo2787 (BvrB) Lmo2788 (BvrA) Similar to A118 prophage Unknown Unknown BLAST result with unfinished...”
BB562_00810 sucrose-specific PTS transporter subunit IIBC from Lactiplantibacillus pentosus
45% identity, 8% coverage
- Transcriptomic Profile and Probiotic Properties of Lactiplantibacillus pentosus Pre-adapted to Edible Oils
Alonso, Frontiers in microbiology 2021 - “...This study greAR ACTGGATATTGGCAAGTTCG BB562_01140 (Cysteine synthase) cysF ATGCTCGTACACCATATCCA 56 200 This study cysR TCAATGATGGTGGTCGTGG BB562_00810 (PTS beta-glucoside transporter subunit EIIBCA) ptsF TTATAAATTAACCGCGGCAG 56 200 This study ptsR ATCAAGGTGACTTACTCGGT BB562_11880 (Transketolase) trakF ATGAACTTAA GCTGAAAGCG 56 199 This study trakR ACCGCATGGCCTTTCGATT pheS (Phenylalanyl-tRNA synthase alpha subunit) pheS-21F...”
- “...for 1,4-alpha-glucan branching enzyme (BB562_00085), glucose-1-phosphate adenylyltransferase (BB562_00090), phosphotransferase system (PTS) beta-glucoside transporter subunit EIIBCA (BB562_00810), sucrose-6-phosphate hydrolase (BB562_00815), and alpha, alpha-phosphotrehalase (BB562_01175) were detected ( Supplementary Table 1 and Supplementary Figure 2 ). Olive Oil Adaptation Changes Nucleotide and Amino Acid Metabolism in L. pentosus...”
CAC1354 Phosphotransferase system IIA component from Clostridium acetobutylicum ATCC 824
46% identity, 36% coverage
- Transcriptional analysis of micronutrient zinc-associated response for enhanced carbohydrate utilization and earlier solventogenesis in Clostridium acetobutylicum
Wu, Scientific reports 2015 - “...encoding a glucose-specific PTS was significantly upregulated accompanied with the other two genes CAC1353 and CAC1354 for glucose transport in the presence of zinc. Additionally, genes involved in the metabolisms of six other carbohydrates (maltose, cellobiose, fructose, mannose, xylose and arabinose) were differentially expressed, indicating that...”
- “...PTS IICBA, was 3.62-fold upregulated. Additionally, the expression of the other two genes CAC1353 and CAC1354 encoding the putative glucose PTS were upregulated by 1.76- and 1.77-fold, respectively. Based on the Q-RT-PCR verification, similar expression patterns of these three genes (3.82-, 2.05- and 2.33-fold upregulation respectively)...”
- Dual substrate specificity of an N-acetylglucosamine phosphotransferase system in Clostridium beijerinckii
Al, Applied and environmental microbiology 2013 - “...acetobutylicum. The proteins encoded by cac1353 (PTS IICB), cac1354 (PTS IIA), and cac1355 (antiterminator) exhibit 41%, 53%, and 51% identity to the...”
- Pleiotropic functions of catabolite control protein CcpA in Butanol-producing Clostridium acetobutylicum
Ren, BMC genomics 2012 - “...gene cluster is likely responsible for fructose metabolism. In contrast, gene glcG (CAC0570), CAC1353 and CAC1354, encoding d -glucose PTS enzyme II components, were all significantly down-regulated after ccpA inactivation (Additional file 5 : Table S4 and Figure 3 ). This observation suggested that CcpA may...”
- “...]. In addition, a CRE site was found in the intergenic region of CAC1353 and CAC1354, two divergently transcribed genes, whereas no such sites were found for gene glcG and ptsI (Additional file 5 ). The results indicated that CcpA might directly regulate either CAC1353 or...”
J3U91_00728 PTS sugar transporter subunit IIA from Oenococcus oeni
46% identity, 33% coverage
BPHYT_RS02740 N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) from Burkholderia phytofirmans PsJN
48% identity, 6% coverage
- mutant phenotype: Specifically important on NAG and cofit with the IIBC component nearby (BPHYT_RS02745). 58% identical to nagF or H16_A0311 of R. eutropha, which is important for NAG utilization (PMCid:PMC3127587). 58% similar to PA3760, which is required for NAG utilization in P. aeruginosa (PMCid:PMC2542419)
MS1508 NagE protein from Mannheimia succiniciproducens MBEL55E
MS1508 PTS glucose transporter subunit IIA from [Mannheimia] succiniciproducens MBEL55E
40% identity, 38% coverage
TC 4.A.1.2.12 / Q8NMD6 The sucrose porter, PtsS (regulated by SugR which also regulates other enzymes II) from Corynebacterium glutamicum (Brevibacterium flavum) (see paper)
NCgl2553 sucrose-specific PTS transporter subunit IIBC from Corynebacterium glutamicum ATCC 13032
cg2925 enzyme II sucrose protein from Corynebacterium glutamicum ATCC 13032
48% identity, 8% coverage
- substrates: Sucrose
- Transcriptome analysis of Corynebacterium glutamicum in the process of recombinant protein expression in bioreactors
Sun, PloS one 2017 - “...is a critical gene in the PTS system, was up-regulated by 2.98-fold, and NCgl1305 and NCgl2553 encoding the phosphotransferase system IIC component were up-regulated by 4.54 and 3.86-fold, respectively, both consistently showing the up absorption and transfer of glucose. Increased glucose consumption during the anaerobic condition...”
- The two-component signal transduction system CopRS of Corynebacterium glutamicum is required for adaptation to copper-excess stress
Schelder, PloS one 2011 - “...cg1290 NCgl1094 metE 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 0.29 cg1487 NCgl1262 leuC isopropylmalate isomerase, large subunit 0.37 cg2925 NCgl2553 ptsS enzyme II sucrose protein 0.38 cg3226 NCgl2816 putative L-lactate permease 0.02 Proteins of unknown function cg0077 NCgl0057 hypothetical protein 11.67 cg0078 NCgl0058 hypothetical protein 10.02 cg0416 NCgl0339 secreted protein...”
- The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum
Gaigalat, BMC molecular biology 2007 - “...PTS component non clustered cg1537 NCgl1305 ptsG 683 72.6 Glucose-specific PTS component, enzyme IIBCA cg2925 NCgl2553 ptsS 661 69.1 Sucrose-specific PTS component, enzyme IIBCA cg3365 NCgl2933 - 513 52.7 L-ascorbate type PTS component, enzyme IIC cg3366 NCgl2934 - 270 29.0 L-ascorbate type PTS component, enzyme IIAB...”
- “...NCBI No. Gene name m-value a-value Annotation cg2026 - - 3.53 11.68 Hypothetical protein cg2925 NCgl2553 ptsS 3.39 11.87 Phosphotransferase system (PTS), sucrose-specific enzyme IIBCA component cg2120 NCgl1861 ptsF 3.27 12.04 Phosphotransferase system (PTS), fructose-specific enzyme IIABC component cg3107 NCgl2709 adhA 3.23 13.00 Alcohol dehydrogenase cg2118...”
- Two-component systems of Corynebacterium glutamicum: deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response
Kocan, Journal of bacteriology 2006 - “...TABLE 1--Continued C. glutamicum locus tag no. NCgl2553 NCgl2717 NCgl2718 NCgl2731 NCgl2787 NCgl2817 NCgl2859 NCgl2860 NCgl2864 NCgl2865 NCgl2877 NCgl2897...”
- Substrate-dependent cluster density dynamics of Corynebacterium glutamicum phosphotransferase system permeases
Martins, Molecular microbiology 2019 - “...in Fig. 1 . ptsG (cg1537) encodes the glucosespecific membrane integral EIIBCA transporter, while ptsS (cg2925) encodes the sucrosespecific EIIBCA, and ptsF (cg2120) the fructosespecific EIIABC (Fig. 1 ). A fourth putative PTS belongs to the Lascorbatetype family and is encoded by rmpC (cg3365) (not depicted...”
- Physiology and Transcriptional Analysis of (p)ppGpp-Related Regulatory Effects in Corynebacterium glutamicum
Ruwe, Frontiers in microbiology 2019 - “...putative iron(III) dicitrate transporter, substrate-binding lipoprotein 1.00 3.76317E06 cg2324 relS (p)ppGpp synthetase, RelA/SpoT-family 1.51 8.38571E77 cg2925 ptsS phosphotransferase system (PTS), sucrose-specific enzyme IIBCA component 1.15 7.96046E51 cg3226 putative MFS-type L-lactate permease 1.22 9.86559E18 log 2 ratios ( M -values) and probability values ( p adj )...”
- The two-component signal transduction system CopRS of Corynebacterium glutamicum is required for adaptation to copper-excess stress
Schelder, PloS one 2011 - “...3.24 cg1290 NCgl1094 metE 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 0.29 cg1487 NCgl1262 leuC isopropylmalate isomerase, large subunit 0.37 cg2925 NCgl2553 ptsS enzyme II sucrose protein 0.38 cg3226 NCgl2816 putative L-lactate permease 0.02 Proteins of unknown function cg0077 NCgl0057 hypothetical protein 11.67 cg0078 NCgl0058 hypothetical protein 10.02 cg0416 NCgl0339 secreted...”
- The Zur regulon of Corynebacterium glutamicum ATCC 13032
Schröder, BMC genomics 2010 - “...4.91 cg2261 amtB secondary ammonium transporter - 1.01 n.s. cg2560 aceA isocitrate lyase 1.56 n.s. cg2925 ptsS phosphotransferase system component - 1.35 n.s. cg3096 ald acetaldehyde dehydrogenase 1.69 103 cg3138 - putative membrane protease subunit 3.14 n.s. cg3139 - hypothetical protein 2.03 n.s. cg3140 tagA1 DNA-3-methyladenine...”
- Elastase digests: new ammunition for shotgun membrane proteomics
Rietschel, Molecular & cellular proteomics : MCP 2009 - “...12 13 14 15 41 39 30 29 28 22 2 17 9 26 Cg0447 Cg2925 Cg2404 Cg1366 Cg3138 16 27 21 Cg2780 17 18 27 26 19 13 Cg2403 Cg2708 19 20 21 22 23 24 25 26 25 23 22 22...”
- The global repressor SugR controls expression of genes of glycolysis and of the L-lactate dehydrogenase LdhA in Corynebacterium glutamicum
Engels, Journal of bacteriology 2008 - “...mutant Gene name, gene product, and/or deduced function cg2925 cg2119 cg2120 cg2118 cg2117 cg2121 cg3219 ptsS, sucrose-specific enzyme II of the PTS pfkB,...”
- Co-ordinated regulation of gluconate catabolism and glucose uptake in Corynebacterium glutamicum by two functionally equivalent transcriptional regulators, GntR1 and GntR2
Frunzke, Molecular microbiology 2008 - “...1.05 1.24 1.34 0.92 cg2399 Glucose kinase, glk 0.22 0.93 0.82 0.66 1.09 0.91 0.81 cg2925 PTS system, sucrose-specific IIABC component, ptsS 0.26 1.05 1.37 1.17 1.47 1.14 1.28 cg2178 N utilization substance protein A 0.26 1.03 0.68 1.09 1.26 0.93 0.85 cg3398 Superfamily II DNA...”
- The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum
Gaigalat, BMC molecular biology 2007 - “...HPr, PTS component non clustered cg1537 NCgl1305 ptsG 683 72.6 Glucose-specific PTS component, enzyme IIBCA cg2925 NCgl2553 ptsS 661 69.1 Sucrose-specific PTS component, enzyme IIBCA cg3365 NCgl2933 - 513 52.7 L-ascorbate type PTS component, enzyme IIC cg3366 NCgl2934 - 270 29.0 L-ascorbate type PTS component, enzyme...”
- “...CDS NCBI No. Gene name m-value a-value Annotation cg2026 - - 3.53 11.68 Hypothetical protein cg2925 NCgl2553 ptsS 3.39 11.87 Phosphotransferase system (PTS), sucrose-specific enzyme IIBCA component cg2120 NCgl1861 ptsF 3.27 12.04 Phosphotransferase system (PTS), fructose-specific enzyme IIABC component cg3107 NCgl2709 adhA 3.23 13.00 Alcohol dehydrogenase...”
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TC 4.A.1.1.15 / Q9HXN5 N-Acetyl-D-Glucosamine phosphotransferase system transporter, component of N-acetyl glucosamine-specific PTS permease, GlcNAc IIBC/GlcNAc I-HPr-IIA from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
PA3760 probable phosphotransferase protein from Pseudomonas aeruginosa PAO1
49% identity, 6% coverage
- substrates: N-acetyl glucosamine
- Impact of transient acquired hypermutability on the inter- and intra-species competitiveness of Pseudomonas aeruginosa
On, The ISME journal 2023 - “...indicated. Multiple ORFs contributed to the peaks and troughs, although a small number of loci (PA3760, encoding a Pts system component (NagF); PA1740, encoding a hypothetical protein; and PA4100, encoding a likely dehydrogenase) appeared to be hotspots for selection. Acquisition of hypermutability confers a fitness advantage...”
- PtsN in Pseudomonas aeruginosa Is Phosphorylated by Redundant Upstream Proteins and Impacts Virulence-Related Genes
Underhill, Journal of bacteriology 2023 (secret) - The development of a new parameter for tracking post-transcriptional regulation allows the detailed map of the Pseudomonas aeruginosa Crc regulon
Corona, Scientific reports 2018 - “...Glycerol-3-phosphate dehydrogenase 2,87 0,16 3,31 Transport PA3690 Probable metal-transporting P-type ATPase 0,46 1,02 3,36 Transport PA3760 N-Acetyl-D-Glucosamine phosphotransferase system transporter 0,5 0,44 1,76 Transport PA3779 Hypothetical protein 0 0,89 2,54 Transport PA3838 Probable ATP-binding component of ABC transporter 0,44 0,49 1,84 Iron fecA PA3901 Fe(III) dicitrate...”
- Pseudomonas aeruginosa enhances production of an antimicrobial in response to N-acetylglucosamine and peptidoglycan
Korgaonkar, Journal of bacteriology 2011 - “...upregulationb GlcNAc catabolism PA3757 PA3758 PA3759 PA3760 PA3761 nagR nagA glmS nagF nagE Probable transcriptional regulator N-Acetylglucosamine-6-phosphate...”
- Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains
Klockgether, Journal of bacteriology 2010 - “...Interg. (PA0369-PA0370) Interg. (PA1352-PA1353) Interg. (PA2046-PA2047) PA3760 PA3877 4448855 4448856 4869855 4924552 4924553 5036891c 5743461 5743462 6098781...”
- “...in our laboratory showed intragenic deletions in PA1695 and PA3760 compared to the published PAO1-UW sequence. Second, the PAO1-DSM subline lacked 2 of the 18...”
- High-throughput phenotypic characterization of Pseudomonas aeruginosa membrane transport genes
Johnson, PLoS genetics 2008 - “...I ( FruI ) PA3597 PAK amino acid APC family transporter PA3641 PAK alanine/sodium symporter PA3760 PAK N-Acetyl-D-Glucosamine acphosphotransferase system transporter PA3761 PAK N-Acetyl-D-Glucosamine phosphotransferase system transporter PA3865 PAK arginine/ornithine ABC transporter periplasmic binding protein PA3889 PAK glycine ABC transporter periplasmic binding protein PA4023 PAK ethanolamine...”
- “...D-Fructose C - PA3562 phosphotransferase system transporter enzyme I ( FruI ) D-Fructose C - PA3760 N-Acetyl-D-Glucosamine phosphotransferase system transporter N-Acetyl-D-Glucosamine N - PA3761 N-Acetyl-D-Glucosamine phosphotransferase system transporter N-Acetyl-D-Glucosamine C+N - PA4910 branched chain amino acid ABC transporter ATP binding protein D-Alanine N +/ PA4912 branched...”
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - “...4833 Q9I5W8 506 Q51390 1588 Q9HXN4 2670 Q9HUV4 3752 Q9I071 4834 Q9I5W9 507 Q51392 1589 Q9HXN5 2671 Q9HUV5 3753 Q9I072 4835 Q9I5X0 508 Q51397 1590 Q9HXN7 2672 Q9HUW2 3754 Q9I074 4836 Q9I5X1 509 Q51425 1591 Q9HXN9 2673 Q9HUW4 3755 Q9I075 4837 Q9I5X2 510 Q51434 1592 Q9HXP9...”
AH67_02320 glucose PTS transporter subunit IIA from Bifidobacterium pseudolongum PV8-2
44% identity, 8% coverage
EFA0067 PTS system, IIABC components from Enterococcus faecalis V583
47% identity, 9% coverage
PA14_15790 putative phosphoenolpyruvate-protein phosphotransferase from Pseudomonas aeruginosa UCBPP-PA14
49% identity, 6% coverage
MNF30_03865 PTS sugar transporter subunit IIA from Mycoplasma mycoides subsp. capri
50% identity, 34% coverage
CTK_C27920 PTS sugar transporter subunit IIA from Clostridium tyrobutyricum
41% identity, 35% coverage
Bbr_1879 PTS sugar transporter subunit IIA from Bifidobacterium breve UCC2003
48% identity, 29% coverage
LSA1792 Sucrose-specific phosphotransferase system, enzyme IIBCA from Lactobacillus sakei subsp. sakei 23K
LCA_RS08985 sucrose-specific PTS transporter subunit IIBC from Latilactobacillus sakei subsp. sakei 23K
40% identity, 11% coverage
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...CG GTTA A A -148 LSA1711 lacL TGAAAC CG TTTT A A -36 lacL-lacM LSA1711-1710 LSA1792 scrA TGTAAA CG GTT GT A -78 scrA-dexB-scrK LSA1792-1790 LSA1830 pox2 T TG TAA CG CTTACA -70 The identification is based on the genome sequence of L. sakei strain 23K,...”
- Transcriptome Analysis Reveals the Role of Sucrose in the Production of Latilactobacillus sakei L3 Exopolysaccharide
Wang, International journal of molecular sciences 2024 - “...sucrose-added medium [ 14 ]. According to Figure 5 and Table 3 , the PTS-S (LCA_RS08985) and PTS fructose transporter subunit IIC (PTS-F, LCA_RS05240), assigned to the phosphotransferase system (PTS) relating to the transport and metabolism of sugars from the extracellular to the intracellular sides of...”
- “...50 ]. The structures of sucrose and PTS sucrose transporter subunit IIABC (PTS-S) encoded by LCA_RS08985 were firstly obtained, and PDBQT files were finally acquired after the optimization of force-field energy by AutodockTools 1.5.6 software [ 51 , 52 ]. 3.3.5. ncRNA Target Genes and Interaction...”
AO353_15995 trehalose-specific PTS system, I, HPr, and IIA components from Pseudomonas fluorescens FW300-N2E3
44% identity, 8% coverage
- mutant phenotype: Specific phenotype on trehalose. The IIB/IIC components are provided by AO353_15980. 45% similar to a NAG PTS system (PA3760, PMC:PMC2542419)
Halsa_1861 PTS sugar transporter subunit IIA from Halanaerobium hydrogeniformans
40% identity, 36% coverage
VC0964 PTS system, glucose-specific IIA component from Vibrio cholerae O1 biovar eltor str. N16961
43% identity, 37% coverage
- Disrupting Central Carbon Metabolism Increases β-Lactam Antibiotic Susceptibility in Vibrio cholerae
Keller, Journal of bacteriology 2023 (secret) - RadD Contributes to R-Loop Avoidance in Sub-MIC Tobramycin
Negro, mBio 2019 - “...VC2669 Tyrosine metabolism 462 78 5.9 0.000 VC0395 gtaB Carbohydrate metabolism 446 53 8.4 0.043 VC0964 crr Carbohydrate metabolism 341 72 4.7 0.004 VC0721 pstS Phosphate ABC transporter 289 71 4.1 0.047 VC1802 Unknown 516 19 27.5 0.011 VC1810 Unknown 508 31 16.5 0.040 Increase VC2326*...”
- Glucose-Specific Enzyme IIA of the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Modulates Chitin Signaling Pathways in Vibrio cholerae
Yamamoto, Journal of bacteriology 2017 - “...order (Fig. 1C): vc0966 (ptsH encoding Hpr), ptsI, and vc0964 (crr encoding EIIAglc). To examine whether EI is the PTS component solely responsible for the...”
- A Genome-Wide Screen Reveals that the Vibrio cholerae Phosphoenolpyruvate Phosphotransferase System Modulates Virulence Gene Expression
Wang, Infection and immunity 2015 - “...O395 toxT C6706 ptsI C6706 ptsH C6706 VC0964 C6706 ptsI lacZ::PtcpA-gfp C6706 ptsH lacZ::PtcpA-gfp C6706 lacZ::PctxAB-gfp C6706 ptsI lacZ::PctxAB-gfp C6706 ptsH...”
- “...VC0966 pCVD442 carrying an unmarked, in-frame deletion in VC0964 pCVD442 carrying an intact gene fragment of VC0965 pCVD442 carrying an intact gene fragment of...”
- (p)ppGpp, a Small Nucleotide Regulator, Directs the Metabolic Fate of Glucose in Vibrio cholerae
Oh, The Journal of biological chemistry 2015 - “...of VC1590 gene (p)ppGpp, Tn inserted in coding region of VC0964 gene (p)ppGpp, Tn inserted in promoter region of VC0964 gene Ref. 13 This study This study This...”
- “...disrupted by Tn insertion were identified as VC0964 (Phosphotransfer system, glucose-specific transporter subunit) and its promoter region, respectively (Fig....”
- Mannitol and the mannitol-specific enzyme IIB subunit activate Vibrio cholerae biofilm formation
Ymele-Leki, Applied and environmental microbiology 2013 - “...operon encoding EI (VC0965), HPr (VC0966), and EIIAGlc (VC0964), is increased (20). To describe differences in gene regulation between this mutant and wild-type...”
- Glucose-specific enzyme IIA has unique binding partners in the vibrio cholerae biofilm
Pickering, mBio 2012 - “...(117) VC0398 MshH 2 22 3 1 VC0900 GGDEF family protein 6 ND ND ND VC0964 EIIA Glc (37) (64) (81) 17 VC1087 Response regulator ND 1 ND ND VC1291 Hypothetical protein 1 10 2 5 VC1655 Mg transporter ND 11 3 ND VC1721 PurR 7...”
- A communal bacterial adhesin anchors biofilm and bystander cells to surfaces
Absalon, PLoS pathogens 2011 - “...inducible promoter and a C-terminal FLAG tag. As negative controls, we also cloned EIIA Glc (VC0964), a cytoplasmic component of the phosphoenolpyruvate phosphotransferase system, as well as Escherichia coli alkaline phosphatase (AP) and TcpG (VC0034), two periplasmic proteins. Each of these plasmids was introduced into V....”
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Lreu_1086 sugar (glycoside-Pentoside-hexuronide) transporter from Lactobacillus reuteri DSM 20016
Lreu_1086 PTS sugar transporter subunit IIA from Limosilactobacillus reuteri subsp. reuteri
38% identity, 11% coverage
GJQ69_01220 PTS transporter subunit IIABC from Caproicibacterium lactatifermentans
47% identity, 7% coverage
LSEI_0631 beta-glucoside-specific PTS system IIABC component from Lactobacillus casei ATCC 334
42% identity, 8% coverage
- New Genes Involved in Mild Stress Response Identified by Transposon Mutagenesis in Lactobacillus paracasei
Palud, Frontiers in microbiology 2018 - “...for heat and salt stress, LSEI_0389 (putative pseudo gene) for heat and acid stress and LSEI_0631 for all stress conditions. For this last gene, encoding a putative PTS system, expressions were over 4.0 for heat (5.47 3.07), salt (4.45 1.11), acid (4.68 0.77), and oxidative (10.20...”
- “...0.46 LSEI_0613 Transposase 3.73 1.61 * 4.62 0.85 * 1.08 0.28 1.20 0.19 1.32 0.,41 LSEI_0631 Beta-glucoside- PTS system 5.47 3.07 * 2.13 0.30 * 4.45 1.11 * 4.68 0.77 * 10.2 3.01 * LSEI_1111 HP 2.97 1.74 * 1.21 0.22 1.79 0.56 * 2.02 0.48...”
MW0241 ORFID:MW0241~hypothetical protein, similar to PTS beta-glucoside-specific enzyme II, ABC component from Staphylococcus aureus subsp. aureus MW2
42% identity, 20% coverage
LGG_00603 PTS system, beta-glucoside-specific IIABC component from Lactobacillus rhamnosus GG
44% identity, 8% coverage
STER_1367, STER_RS06730, T303_07870 PTS sugar transporter subunit IIA from Streptococcus thermophilus LMD-9
42% identity, 9% coverage
- Identification of Hanks-Type Kinase PknB-Specific Targets in the Streptococcus thermophilus Phosphoproteome
Henry, Frontiers in microbiology 2019 - “...STER_RS09185 LacZ Beta-galactosidase YP_820735.1 WP_011226267.1 STER_1366 STER_RS06725 LacS PTS sugar transporter subunit IIA YP_820736.1 WP_011681393.1 STER_1367 STER_RS06730 GalM Galactose mutarotase YP_820737.1 WP_011226269.1 STER_1368 STER_RS06735 GalE UDP-glucose 4-epimerase YP_820738.1 WP_011681394.1 STER_1369 STER_RS06740 PpnK NAD kinase YP_820780.1 WP_014608552.1 STER_1422 STER_RS06995 RgpA Glycosyltransferase family 1 YP_820795.1 WP_011681439.1 STER_1437 STER_RS07070...”
- “...LacZ Beta-galactosidase YP_820735.1 WP_011226267.1 STER_1366 STER_RS06725 LacS PTS sugar transporter subunit IIA YP_820736.1 WP_011681393.1 STER_1367 STER_RS06730 GalM Galactose mutarotase YP_820737.1 WP_011226269.1 STER_1368 STER_RS06735 GalE UDP-glucose 4-epimerase YP_820738.1 WP_011681394.1 STER_1369 STER_RS06740 PpnK NAD kinase YP_820780.1 WP_014608552.1 STER_1422 STER_RS06995 RgpA Glycosyltransferase family 1 YP_820795.1 WP_011681439.1 STER_1437 STER_RS07070 AmyL...”
- Functional Genomic Analyses of Exopolysaccharide-Producing Streptococcus thermophilus ASCC 1275 in Response to Milk Fermentation Conditions
Wu, Frontiers in microbiology 2019 - “...1.79 Branched-chain amino acid aminotransferase Carbohydrate transport and metabolism T303_04850 0.62 Glucokinase T303_02705 0.53 Transketolase T303_07870 0.49 Lactose/galactose permease T303_06080 5.42 Maltodextrin phosphorylase T303_06085 5.00 4-alpha-glucanotransferase Stress response T303_00885 17.90 Hypothetical protein General stress response T303_07635 4.43 Hypothetical protein General stress response T303_04835 4.77 DNA protection...”
- “...ST1275 Lactose is the major carbon source in milk. It is observed that the LacS (T303_07870; lactose permease) was about 2-fold down regulated in ST1275 under Cd2 compared to that of Cd1 ( Table 1 ). Our previous study showed that about 99% lactose was utilized...”
- Comparative mRNA-Seq Analysis Reveals the Improved EPS Production Machinery in Streptococcus thermophilus ASCC 1275 During Optimized Milk Fermentation
Wu, Frontiers in microbiology 2018 - “...serine proteinase 94 5205-F AACCACAGTCAGGCAATAAG 5205-R CTGTCTGTCCCATTCCATTC T303_05515 Glucosaminefructose-6-phosphate aminotransferase 111 5515-F CCAGTTGAGCTCGGTATTTC 5515-R CTGACGACTATCTGCTGTTTC T303_07870 Lactose permease 100 7870-F TGGCGAGTAGGAAGAATTTG 7870-R CTTCAGGTAGCATGGGTAAAG T303_09015 Universal stress protein UspA 95 9015-F GTGCCACTGGTCTCAATAC 9015-R ACGAACAACGAGCAAGTC Cd4 vs. Cd2 T303_02960 Cysteine synthase A 105 2960-F CAGCTGGATTTGAAGGGTTAG 2960-R CTGGTAGCGAAGGAATGAAAG T303_03905...”
- Transcriptomic Insights Into the Growth Phase- and Sugar-Associated Changes in the Exopolysaccharide Production of a High EPS-Producing Streptococcus thermophilus ASCC 1275
Padmanabhan, Frontiers in microbiology 2018 - “...CCTGCCTCCTTTCATCATC CAACTCCAAGCTCTACTTCTAC 1.67 1.39 T303_06885 Phosphoglycerate mutase 162 GTACTCAGCACACAAAGACC CCATGTGCACCTACGAATAC 1.37 1.25 L5h vs. L10h T303_07870 Lactose/galactose permease 155 CTTCAGGTAGCATGGGTAAAG GATGCCAACGTGGATAAGAA 2.01 2.21 T303_03155 6-phosphofructokinase 124 GACAAGGTAGAGATTGGTGAAG TGAAGCCCGTAGCAGTAT 3.39 2.96 T303_06085 4-alpha-glucanotransferase 156 CGTCAAGGTGAACCAATCA CATACGCCATCTCCAGTTTC 3.90 3.37 T303_08070 Peptide ABC transporter ATP-binding protein 162 CTGCGGCTAGTTTGAATGA CGTGAGGGTAACGTGTTAAG...”
TC 2.A.2.2.1 / P23936 Lactose permease, LacS. Mediates uptake of β-galactooligosaccharides, lactitol, and a broad range of prebiotic β-galactosides that selectively stimulate beneficial gut microbiota from Streptococcus thermophilus (see 2 papers)
42% identity, 9% coverage
- substrates: Beta-galactooligosaccharides, Lactitol, Lactose
- Cloning, characterization, and functional expression of the Klebsiella oxytoca xylodextrin utilization operon (xynTB) in Escherichia coli
Qian, Applied and environmental microbiology 2003 - “...LacS (AAL67293); Strth, S. thermophilus A147, LacS (P23936); Sty, S. enterica subsp. enterica serovar Typhi, STY0171 (NP_454763), YicJ (NP_458178), YihP...”
CC0448 PTS system, fructose-specific EIIA/HPr/EI components from Caulobacter crescentus CB15
46% identity, 6% coverage
Blon_2470 PTS system, glucose subfamily, IIA subunit from Bifidobacterium longum subsp. infantis ATCC 15697
46% identity, 29% coverage
Blon_2183 PTS system, glucose subfamily, IIA subunit from Bifidobacterium longum subsp. infantis ATCC 15697
43% identity, 8% coverage
- Strain-specific strategies of 2'-fucosyllactose, 3-fucosyllactose, and difucosyllactose assimilation by Bifidobacterium longum subsp. infantis Bi-26 and ATCC 15697
Zabel, Scientific reports 2020 - “...a transposase insertion in the antiterminator required for activity of the -glucoside-specific PTS transport system (Blon_2183) involved in recognition and uptake of glucose from the external environment 26 . This transposase is not present in Bi-26, but present in the type strain and several other strains...”
- “...insertion within predicted antiterminator of BglG family (Blon_2180) had inactivated the predicted glucose PTS transporter Blon_2183. Furthermore, the small number of DEGs implies that there are no other functional transport systems for glucose uptake in the type strain making this strain technically blind to the presence...”
- Release and utilization of N-acetyl-D-glucosamine from human milk oligosaccharides by Bifidobacterium longum subsp. infantis
Garrido, Anaerobe 2012 - “...induced by glucose, probably associated to another PTS system that imports glucose in B. infantis (Blon_2183). 4. Discussion Certain infant-borne Bifidobacterium species are known to efficiently consume HMO, one of the most abundant components of human milk [ 20 , 22 ]. B. infantis is characterized...”
- Analysis of predicted carbohydrate transport systems encoded by Bifidobacterium bifidum PRL2010
Turroni, Applied and environmental microbiology 2012 - “...Bbr_1589 Bbr_1588 Bbr_1111 Bbr_1880 Bbr_0101 Bbr_1892 Blon_2183 Blon_2331 Blon_0342 Blon_2015 Blon_2177 Blon_2176 Blon_2175 Blon_1050 Blon_2471 Blon_0129 B....”
LGAS_1778 Sucrose PTS, EIIBCA from Lactobacillus gasseri ATCC 33323
39% identity, 11% coverage
- The PTS transporters of Lactobacillus gasseri ATCC 33323
Francl, BMC microbiology 2010 - “...PTS 15, 20, and 21, respectively (Table 5 ). The amplified PTS 15 (LGAS_1669), 20 (LGAS_1778) and 21 (LGAS_1795) internal regions were 819 bp, 760 bp and 675 bp, respectively. The junction fragments for successful pMJM-4 integration were 999 bp and 1039 bp. The junction fragments...”
SAV2538 PTS system, glucose-specific II ABC component from Staphylococcus aureus subsp. aureus Mu50
SA2326 PTS system, glucose-specific IIABC component from Staphylococcus aureus subsp. aureus N315
39% identity, 10% coverage
- Genome-wide analysis of the regulatory function mediated by the small regulatory psm-mec RNA of methicillin-resistant Staphylococcus aureus
Cheung, International journal of medical microbiology : IJMM 2014 - “...MSA3407, up-regulated 02548 (COL) Protein A 43.2 SAV2377 (Mu50) PTS system, sucrose-specific IIBC component 20.7 SAV2538 (Mu50) PTS system, glucose-specific II ABC component 16.4 SAV2507 (Mu50) Gluconate operon transcriptional repressor 14.1 SAV1068 (Mu50) Phosphoribosylformylglycinamidine synthase I 11.5 SAV1065 (Mu50) Phosphoribosylaminoimidazole carboxylase 10.1 MSA3407, down-regulated SAV1330 (Mu50)...”
- Two novel point mutations in clinical Staphylococcus aureus reduce linezolid susceptibility and switch on the stringent response to promote persistent infection
Gao, PLoS pathogens 2010 - “...lacC tagatose-6-phosphate kinase 0.01 SAV2194 lacB galactose-6-phosphate isomerase 0.03 SAV2256 glucose uptake protein homolog 0.5 SAV2538 ptsG PTS system, glucose-specific II ABC component 0.3 Up-regulated MW2435 fructose-bisphosphatase (fbp) 2.2 SAS0431 sugar-specific PTS transport system, IIBC component (trehalose) 2.2 SAS1448 maltose operon transcriptional repressor 7.5 SAV0247 gatC...”
- Exposure of clinical MRSA heterogeneous strains to β-lactams redirects metabolism to optimize energy production through the TCA cycle
Keaton, PloS one 2013 - “...( glcA , SA0183), putative fructose specific PTS (SA2434), glucoside specific PTS ( ptsG , SA2326) and glucose specific PTS (SA1566). A group of hypothetical proteins predicted to be related to carbohydrate metabolism were downregulated during HeR/HoR selection, including lactate permease ( lctP , SA0106), probable...”
- Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States
Tenover, Journal of clinical microbiology 2006 - “...dehydrogenase (associated with vancomycin resistance) SA2326 Fosfomycin resistance protein SA2413 Drug resistance transporter SA0772 Aluminum resistance...”
- Global regulation of Staphylococcus aureus genes by Rot
Saïd-Salim, Journal of bacteriology 2003 - “...SA2208 SA1881 SA1879 SA1090 SA0549 SA2334 SA2185 SA2435 SA1659 SA2326 SA1589 SA1631 SA1630 SA1629 SA1628 2064 2067 2238 2543 2612 2860 2926 3017 3078 3373 3768...”
TC 4.A.1.2.16 / ART98386 PTS beta-glucoside transporter, EIIBCA of 647 aas and 10 predicted TMSs from Lactobacillus gasseri
39% identity, 11% coverage
- substrates: beta glucosides
1glcF / P69783 Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
43% identity, 39% coverage
stu1398 lactose permease from Streptococcus thermophilus LMG 18311
str1398 sodium:beta-glucoside symporter from Streptococcus thermophilus CNRZ1066
42% identity, 9% coverage
- Specialized adaptation of a lactic acid bacterium to the milk environment: the comparative genomics of Streptococcus thermophilus LMD-9
Goh, Microbial cell factories 2011 - “...component, trunc. stu0201 str0201 Sugar permease STER0891 Glucose/ribose permease stu0855 str0855 STER1367 Lactose permease, LacS stu1398 str1398 Sugar hydrolase STER1366 Beta-galactosidase, LacZ stu1397 str1397 STER1500 Alpha-amylase (cytoplasmic) stu1542 str1542 STER1711 Sucrose-6-phosphate hydrolase stu1735 str1735 STER1029 Putative pullulanase, trunc. STER1049 Beta-glucosidase, trunc. stu1064 str1064 STER1594 6-phospho-beta-glucosidase, trunc....”
- Specialized adaptation of a lactic acid bacterium to the milk environment: the comparative genomics of Streptococcus thermophilus LMD-9
Goh, Microbial cell factories 2011 - “...trunc. stu0201 str0201 Sugar permease STER0891 Glucose/ribose permease stu0855 str0855 STER1367 Lactose permease, LacS stu1398 str1398 Sugar hydrolase STER1366 Beta-galactosidase, LacZ stu1397 str1397 STER1500 Alpha-amylase (cytoplasmic) stu1542 str1542 STER1711 Sucrose-6-phosphate hydrolase stu1735 str1735 STER1029 Putative pullulanase, trunc. STER1049 Beta-glucosidase, trunc. stu1064 str1064 STER1594 6-phospho-beta-glucosidase, trunc. stu1631...”
TC 4.A.1.2.15 / ART98417 PTS beta-glucoside transporter, EIIBCA of 624 aas and 10 predicted TMSs from Lactobacillus gasseri
41% identity, 11% coverage
- substrates: beta-glucosides
Crr / b2417 Enzyme IIAGlc (EC 2.7.1.199; EC 2.7.1.201; EC 2.7.1.192) from Escherichia coli K-12 substr. MG1655 (see 47 papers)
Crr / P69783 Enzyme IIAGlc (EC 2.7.1.199) from Escherichia coli (strain K12) (see 46 papers)
PTGA_ECOLI / P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
P69783 protein-Npi-phosphohistidine-D-glucose phosphotransferase (EC 2.7.1.199) from Escherichia coli (see 2 papers)
TC 4.A.1.1.1 / P69783 Glucose-specific phosphotransferase enzyme IIA component PTGA aka CRR aka GSR aka IEX aka TGS aka TRED aka B2417, component of Glucose porter (PtsG; GlcA; Umg) (transports D-glucose and α-methyl-D-glucopyranoside) from Escherichia coli (see 16 papers)
crr / GB|AAC75470.1 glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- from Escherichia coli K12 (see 16 papers)
b2417 glucose-specific PTS system enzyme IIA component from Escherichia coli str. K-12 substr. MG1655
NP_416912 Enzyme IIA(Glc) from Escherichia coli str. K-12 substr. MG1655
43% identity, 37% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (PubMed:17158705, PubMed:3129430). The enzyme II complex composed of PtsG and Crr is involved in glucose transport (PubMed:2657735). The non-phosphorylated EIII-Glc is an inhibitor for uptake of certain sugars such as maltose, melibiose, lactose, and glycerol. Phosphorylated EIII-Glc, however, may be an activator for adenylate cyclase. It is an important regulatory protein for cell metabolism (PubMed:789369).
cofactor: Zn(2+) (Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization.)
subunit: Heterodimer with glycerol kinase (glpk).
disruption phenotype: In mutants defective in enzyme I and histidine- containing phosphate carrier protein (HPr), cells lacking this gene are able to grow on the non-PTS compounds such as glycerol, maltose, melibiose, mannose 6-phosphate, and alpha-glycerol phosphate. - substrates: Alpha-methyl-D-glucopyranoside, D-glucose
tcdb comment: The IIC domain has been crystallized, and x-ray data to 4.5 Å resolution have been described (Zurbriggen et al. 2010). The system has been manipulated to engineer increased production of aromatic metabolites (Carmona et al. 2015, Vargas-Tah et al. 2015). The presence or absence of D-glucose reflects the transporter before and after release of the transported glucose into the cytoplasm. The transition associated with substrate release appears to require a subtle structural rearrangement in the region that includes hairpin 1 (Kalbermatter et al. 2017). Mlc (for makes large colonies) represses transcription of the genes encoding enzyme I, HPr and EIIBCGlc in defined media that lack PTS substrates. When glucose is present, the unphosphorylated form of EIIBCGlc sequesters Mlc to the cell membrane, preventing its interaction with DNA (Plumbridge 2002, Joyet et al. 2013). The Vibrio Mlc functions similarly (Pickering et al. 2014). A small (43 aa) protein, SgrT, acts in tandem with a well-characterized small RNA during metabolic stress, due to the accumulation of cytoplasmic sugar-Ps to help bacterial cells maintain balanced metabolism and continue growing. SgrT acts on the glucose transport system, inhibiting its activity under stress conditions in order to allow cells to utilize alternative carbon sources (Lloyd et al. 2017). ptsG mRNA localization to the inner membrane, coupled with the membrane insertion of nascent peptide, mediates Hfq/SgrS-dependent ptsG mRNA destabilization, presumably by reducing second rounds of translation (Kawamoto et al. 2005) - FastKnock: an efficient next-generation approach to identify all knockout strategies for strain optimization
Hassani, Microbial cell factories 2024 - “...b1723 Triple ACKr, GLCpts, PYK 0.56 54.88 b2296, b3115, b1849, b1819, b2415, b2416, b1621, b1101, b2417, b1817, b1818, b1854, b1676 DHAPT, GART, PPAKr Quadruple ACKr, ARGDC, GLCpts, PYK 0.56 64.72 b2296, b3115, b1849, b2938, b4117, b1819, b2415, b2416, b1621, b1101, b2417, b1817, b1818, b1854, b1676 GART,...”
- The association of DNA damage response and nucleotide level modulation with the antibacterial mechanism of the anti-folate drug trimethoprim
Sangurdekar, BMC genomics 2011 - “...Function unknown ymfQ b1153 Function unknown yobG b1826 Function unknown yecN b1869 Function unknown crr b2417 Phosphocarrier protein for glucose of the PTS; Enzyme IIA(Glc); formerly EIII(glc) recJ b2892 Single-stranded DNA-specific exonuclease, 5'-3' ygjT b3088 Function unknown; induced by alkali; putative membrane transport or efflux protein...”
- E Unibus Plurum: genomic analysis of an experimentally evolved polymorphism in Escherichia coli
Kinnersley, PLoS genetics 2009 - “...1.7 0.8 0.0 reg. of Cpx response cpxP Cell processes; Adaptations; Regulation; 2-component regulatory system b2417 crr 1-class 0.9 0.5 1.1 1.1 glucose-specific enzyme IIA component of PTS ptsHI-crr (ptsHp1) Metabolism; carbon utilization; The PTS Fructose-Mannitol (Fru) Family, transport; substrate; D-glucose/trehalose b1073 flgB 4-class 1.3 1.1...”
- Experimental and computational assessment of conditionally essential genes in Escherichia coli
Joyce, Journal of bacteriology 2006 - “...metR (b3828) Transport ....................................................................... crr (b2417) cysA (b2422) cysU (b2424) [fes (b0585)] ptsI (b2416)...”
- “...atpF (b3736); atpG (b3733); atpH (b3735) crr (b2417); glpK (b3926); ppc (b3956); ptsI (b2416); fruR (b0080) Ubiquinone biosynthesis Pyridoxine biosynthesis...”
- Regulation of CsrB/C sRNA decay by EIIA(Glc) of the phosphoenolpyruvate: carbohydrate phosphotransferase system.
Leng, Molecular microbiology 2016 - GeneRIF: results of a detailed investigation of the role of EIIAGlc in CsrB/C decay; unphosphorylated EIIAGlc binds specifically to the EAL domain of CsrD and stimulates CsrB/C turnover
- Sugar Influx Sensing by the Phosphotransferase System of Escherichia coli.
Somavanshi, PLoS biology 2016 - GeneRIF: Results suggest that the phosphoenolpyruvate hexose phosphotransferases PTS network serves as a global sensor of the sugar influx and also monitors the levels of several non-PTS compounds.
- Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography.
Chen, Nature 2013 - GeneRIF: The data suggest a model of how the central regulatory protein EIIA(Glc) allosterically inhibits maltose uptake in E. coli.
- Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression.
Han, Biochimica et biophysica acta 2007 (PubMed)- GeneRIF: Transport proteins PotD and Crr of Escherichia coli, novel fusion partners for heterologous protein expression.
- Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database.
Pasquali, Electrophoresis 1996 (PubMed)- GeneRIF: N-terminus verified by Edman degradation on mature peptide
- Antimicrobial Mechanism and Identification of the Proteins Mediated by Extracts from Asphaltum punjabianum and Myrtus communis
Aabed, ACS omega 2020 - “...3.6610 4 N.S. 2.77 DOWN 5 P08839 phosphoenolpyruvate-protein phosphotransferase PT1_ECOLI 0.009 N.S. 5.18 UP 6 P69783 PTS system glucose-specific EIIA component PTGA_ECOLI 0.013 N.S. 1.5 DOWN 7 Q6BEX0 galactofuranose transporter ATP-binding protein YtfR YTFR_ECOLI 0.004 N.S. 1.61 UP 8 P09373 formate acetyltransferase1 PFLB_ECOLI 0.033 N.S. DOWN...”
- Quantification of Lysine Acetylation and Succinylation Stoichiometry in Proteins Using Mass Spectrometric Data-Independent Acquisitions (SWATH)
Meyer, Journal of the American Society for Mass Spectrometry 2016 - “...of 0.5% was determined for acetylated peptide GF Kac RIAE representing K-105 in protein PTGA (P69783). The MS/MS is shown for the doubly charged light precursor ion at m/z 431.74. Fragment ion XICs derived from the corresponding light and heavy precursor ions at m/z 431.74 and...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...Protein nameb Crr Accession no.b P69783 Descriptionb pI/MW Theoreticalc Glucose-specific phosphotransferase enzyme IIA component 4.73/18,119.88 P0A9X9...”
BCUN_1552 glucose PTS transporter subunit IIA from Bifidobacterium cuniculi
44% identity, 8% coverage
Bbr_1594 glucose PTS transporter subunit IIA from Bifidobacterium breve UCC2003
43% identity, 8% coverage
- Bifidobacterial biofilm formation is a multifactorial adaptive phenomenon in response to bile exposure
Kelly, Scientific reports 2020 - “...tag Putative Function Fold change in expression (Up regulation) P-value Bbr_0376 Hypothetical protein 10.08 9.18E14 Bbr_1594 PTS system fructose/glucose ( fruA ) 6.09 4.99E15 Bbr_0925 Permease MFS superfamily 5.49 0.000125407 Bbr_0204 Multi-domain protein fatty acids or polyketide synthesis 4.33 1.79E10 Bbr_1558 Permease of ABC transporter 4.16...”
- “...significantly upregulated following 0.5% bile exposure. Transcription of a gene encoding a putative PEP-PTS system (Bbr_1594), which previously was shown to be induced by glucose 48 , was upregulated sixfold under the imposed bile exposure conditions. Similarly, genes predicted to encode an ABC-type transporter permease (Bbr_1558),...”
- Comparative genomics and genotype-phenotype associations in Bifidobacterium breve
Bottacini, Scientific reports 2018 - “...Bbr_100, an ABC transporter Bbr_0026-27, an additional -fructofuranosidase Bbr_1324 and a fructose phosphotransferase PTS system Bbr_1594) 23 , 28 , 42 . Of the 35 substrates for which differential growth was observed among B . breve strains, some of these (e.g. starch and starch-like polysaccharides, lacto-...”
- Analysis of predicted carbohydrate transport systems encoded by Bifidobacterium bifidum PRL2010
Turroni, Applied and environmental microbiology 2012 - “...BL_1163 BL_1638 BL_1639 BL_1640 BL_1498 BL_0534 Bbr_1594 Bbr_1551 Bbr_0528 Bbr_0531 Bbr_1590 Bbr_1589 Bbr_1588 Bbr_1111 Bbr_1880 Bbr_0101 Bbr_1892 Blon_2183...”
BAD_RS01940 glucose PTS transporter subunit IIA from Bifidobacterium adolescentis ATCC 15703
43% identity, 9% coverage
- Combining of transcriptome and metabolome analyses for understanding the utilization and metabolic pathways of Xylo-oligosaccharide in Bifidobacterium adolescentis ATCC 15703
Yang, Food science & nutrition 2019 - “...transporter permease F:CTTCGTGCCGTATGTCGTTT 58.7 246 R:TGGCTGCTTCATACAGTTCC 57.9 BAD_RS00875 Phosphoenolpyruvateprotein phosphotransferase F:TGTTCCGTACCGAATTCCTGT 59.5 117 R:TGCGGATCACGACCTTCTT 58.5 BAD_RS01940 PTS betaglucoside transporter subunit EIIBCA F:ACGCTCGGCTACGACTTCAT 59.9 200 R:AGCTTGTACCGCAGGTGGAT 58.5 BAD_RS06365 Betaglucosidase F:CTTCTACATCACCGCCTACCA 57.8 167 R:TATCGAGGACCACGTTCTTAAA 57.2 BAD_RS02270 Betaxylosidase F:CCAGCCAGCTTGATATGAGAG 57.6 154 R:TCGGCGGTGACCAAATAA 57.8 BAD_RS03990 Glutamate synthase [NADPH] large subunit...”
- “...& Sinderen, 2007 ). Compared with xylose treatment, PTS betaglucoside transporter subunit EIIBCA (encoded by BAD_RS01940) and phosphoenolpyruvateprotein phosphotransferase (encoded by BAD_RS00875) were downregulated in B.adolescentis 15703 grown on XOS. Meanwhile, metabolites including upregulated DSorbitol 6phosphate (meta_761), Dmannose (meta_166) and downregulated Lascorbic acid (meta_312), DGlucose 6phosphate...”
BBMN68_1665 glucose PTS transporter subunit IIA from Bifidobacterium longum subsp. longum BBMN68
43% identity, 7% coverage
PMI1830 PTS family enzyme IIA component from Proteus mirabilis HI4320
41% identity, 37% coverage
AH68_02050 glucose PTS transporter subunit IIA from Bifidobacterium catenulatum PV20-2
46% identity, 8% coverage
EF0958 PTS system, IIABC components from Enterococcus faecalis V583
43% identity, 8% coverage
- The Coexistence of Bacterial Species Restructures Biofilm Architecture and Increases Tolerance to Antimicrobial Agents
Dong, Microbiology spectrum 2023 - “...transporter subunit IIA components b +8.9 ef0541 PTS transporter subunit EIIC components +3.2 +5.1 Maltose ef0958 PTS system EIICB components 2.6 +1.2 ef0960 6-phosphate phosphatase 2.3 +1.7 Galactose gatA PTS system EIIA components +1.3 +2.3 gatC PTS system EIIC components 3.8 +3.2 Mannose ef0815 PTS system...”
- Class IIa bacteriocin resistance in Enterococcus faecalis V583: the mannose PTS operon mediates global transcriptional responses
Opsata, BMC microbiology 2010 - “...of energy metabolism in these organisms. However, notable differences were also seen; PTS for trehalose (EF0958) and a gene for sodium-iron-translocating uptake (EF3324), both downregulated in the S. mutans mutant were up regulated in the E. faecalis mutants. Moreover, central glycolytic genes showed an opposite regulation...”
- Comparative genomic analysis of pathogenic and probiotic Enterococcus faecalis isolates, and their transcriptional responses to growth in human urine
Vebø, PloS one 2010 - “...formation would be modulated by human urine. The gene encoding the maltose PTS system malT (EF0958) and the cognate operon bopABCD / malPBMR (EF0957-54), are involved in biofilm formation [77] , [78] , and were partly up-regulated in OG1RF at t 5 . Another gene important...”
- The transcriptome of the nosocomial pathogen Enterococcus faecalis V583 reveals adaptive responses to growth in blood
Vebø, PloS one 2009 - “...metabolism 2.5 [54] EF0957 glycosyl hydrolase, family 65 ( malA/bopA ) Energy metabolism 2.5 [54] EF0958 PTS system, IIABC components ( malT ) Signal transduction 2.2 [54] EF1051 sensor histidine kinase ( etaS ) Signal transduction 2.6 [70] EF1211 NADH peroxidase ( npr ) Oxidative stress...”
- “...one operon, the bopABCD / malPBMR operon (EF0957-54), and a phosphoenolpyruvate phosphotransferase system (PTS) malT (EF0958) of which the genes malT and bopAB/malPB were up-regulated in blood. The genes bopCD/malMR showed the same trend, although not statistically significant. Furthermore, the secreted antigen salB (EF0394) showed reduced...”
- Comparison of OG1RF and an isogenic fsrB deletion mutant by transcriptional analysis: the Fsr system of Enterococcus faecalis is more than the activator of gelatinase and serine protease
Bourgogne, Journal of bacteriology 2006 - “...system EF0411 EF0412 EF0413 EF0955 EF0956 EF0957 EF0958 EF3210 EF3211 EF3212 EF3213 Mannitol-specific IIBC components mltF mtlD bopC/malMd bopB/malB bopA/malP...”
- “...EF0104 EF0105 EF0106 ENT-stat 2882 BOURGOGNE ET AL. (ef0958) and malPBMR (ef0957 to -0954) (24). Both malT (ef0958) and malP (ef0957/bopA) appear to be...”
- Transcriptional response of Enterococcus faecalis V583 to erythromycin
Aakra, Antimicrobial agents and chemotherapy 2005 - “...EF2066 EF2259 EF2653 EF2852 EF3272 EF0021 EF0022 EF0717 EF0958 EF1051 EF1261 EF1516 EF2978 EF2979 EF0846 EF1170 EF3214 EF0188 EF0636 EF0744 EF0760 EF0789 EF0790...”
- “...and binding protein EF0020 EF0021 EF0022 EF0411 EF0553 EF0958 EF1012 EF1017 EF1018 EF1019 EF1336 EF1633 EF1801 EF2213 EF3029 EF3290 EF0782 EF3180 EF0056 EF0179...”
OG1RF_10684 PTS transporter subunit IIBC from Enterococcus faecalis OG1RF
43% identity, 8% coverage
- Genome-wide analysis of <i>Enterococcus faecalis</i> genes that facilitate interspecies competition with <i>Lactobacillus crispatus</i>
Lam, Journal of bacteriology 2025 - “...and carbon utilization/uptake OG1RF_10030 ABC superfamily ATP binding cassette transporter permease subunit 2.6351 5.32E-05 1.82E-04 OG1RF_10684 Maltose PTS enzyme II 2.2417 6.60E-08 1.00E-06 OG1RF_10991 Spermidine/putrescine ABC transporter permease protein 3.2017 2.08E-05 8.18E-05 OG1RF_10992 Spermidine/putrescine ABC transporter ATP-binding protein 3.7275 2.59E-05 9.94E-05 OG1RF_10993 Spermidine/putrescine ABC transporter substrate-binding...”
- Global Regulation of Gene Expression by the MafR Protein of Enterococcus faecalis
Ruiz-Cruz, Frontiers in microbiology 2015 - “...3) Reverse primer (5 to 3) OG1RF_10298 GGCGTTCATTACGTTGCTGA TCGCTTCGTCAATGGTTTGAT OG1RF_10456 AGACGCCAATTTGTTAGAACG CACAACTAGCGGCTAAAGAAG OG1RF_10683 CAGAAGATGGCTTACACATTACCT GAGTAGGCTGTCCATGTCGCT OG1RF_10684 TAATGGTCGTTGTGGCAGTA AATTGCCCAACCGATACTCT OG1RF_11135 CGTTCGTAGTTTTGCTGTCA GAAGGGACAAAGCCGATTTCT OG1RF_11146 GAGGGTGGCTTTAGTGGAGA TTCACCTTCTGCTACGACTT OG1RF_11592 AACAAGCCGCCTTATTTGGT GGTTCTTCGCCAGTGTTCAT OG1RF_11763 AACATCGGCGGTATCTTCAG ATGCCTACATCACCAGTAGC OG1RF_11948 CTTGTACTGATGTGACTGGGTT CCAACGCTCCTGTAATGGTT OG1RF_12398 CCCGACGAATGATTTGCCTA ATCTAACGAACCAGCGACACT OG1RF_12405 GAAGCATTGCGTTTGGAGAT AGAAGCGACCACTTTGTTTG OG1RF_12439 GCAACGAAATGGTGGAACAG AAGGCATCGGCAATCTCTAAG OG1RF_12564 ACTTGTTCGTGGACGGATTC TGCAATGCCAACTTCTGTTA Growth...”
- “...OG1RF_10681 ( malM ) and OG1RF_10680 ( malR ). The malPBMR operon and its neighboring OG1RF_10684 gene ( malT , PTS transporter) are divergently transcribed and were shown to be involved in maltose utilization ( Le Breton et al., 2005 ). The malPBMR operon was originally...”
PS417_23035 D-trehalose PTS system, I, HPr, and IIA components from Pseudomonas simiae WCS417
40% identity, 7% coverage
- mutant phenotype: Specific phenotype for trehalose and no other trehalose transporter is apparent in the fitness data. The IIB and IIC components are provided by PS417_23050
PTGA_SALTY / P0A283 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 3 papers)
SPC_1226 glucose-specific PTS system enzyme IIA component from Salmonella enterica subsp. enterica serovar Paratyphi C strain RKS4594
STM2433 PTS family, glucose-specific IIA component from Salmonella typhimurium LT2
41% identity, 37% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of PtsG and Crr is involved in glucose transport (PubMed:6292227, PubMed:6754734). It can also phosphorylate mannose, methyl alpha-glucoside and 2-deoxy-glucose (PubMed:6292227). The non-phosphorylated EIII-Glc is an inhibitor for uptake of certain sugars such as maltose, melibiose, lactose, and glycerol. Phosphorylated EIII-Glc, however, may be an activator for adenylate cyclase (PubMed:789369).
cofactor: Zn(2+) (Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization.)
subunit: Heterodimer with glycerol kinase (glpk).
disruption phenotype: In mutants defective in enzyme I and histidine- containing phosphate carrier protein (HPr), cells lacking this gene are able to grow on the non-PTS compounds such as glycerol, maltose, melibiose, mannose 6-phosphate, and alpha-glycerol phosphate. - Antibacterial Components and Modes of the Methanol-Phase Extract from Commelina communis Linn
Liu, Plants (Basel, Switzerland) 2023 - “...E1 component Glycolysis/Gluconeogenesis SPC_3760 0.06 Aldehyde dehydrogenase B SPC_4566 0.241 Fructose-1-bisphosphatase SPC_4282 0.417 Glucose-6-phosphate isomerase SPC_1226 2.146 Glucose-specific PTS system enzyme IIA component SPC_4187 2.296 Triosephosphate isomerase SPC_1980 2.76 Alcohol dehydrogenase SPC_2451 3.023 Glyceraldehyde-3-phosphate dehydrogenase SPC_3111 3.382 6-phospho-beta-glucosidase SPC_2543 5.68 PTS system glucose-specific IIBC component Oxidative...”
- N-dodecanoyl-homoserine lactone influences the levels of thiol and proteins related to oxidation-reduction process in Salmonella
de, PloS one 2018 - “...-9.017 1.957 -1.073 0.737 -0.934 0.522 9.351 1.331 0.646 0.920 PTS system glucose-specific EIIA component P0A283 crr Transport -0.304 0.841 -2.267 3.151 -1.073 1.822 -0.399 1.485 0.063 0.257 Multiphosphoryl transfer protein P17127 fruB Transport ND ND -7.368 1.542 ND ND ND ND ND ND Phosphocarrier protein...”
- Salmonella enterica serovar typhimurium colonizing the lumen of the chicken intestine grows slowly and upregulates a unique set of virulence and metabolism genes
Harvey, Infection and immunity 2011 - “...2.6 0.0036 0.01 0.03 Carbohydrate transport and metabolism STM2433 STM4231 STM2190 STM0684 STM0685 STM2431 crr lamB mglB nagB nagE ptsH Glucose-specific IIA...”
- “...and metabolism STM0310 STM1558 STM2431 STM2433 gmhA ptsH crr Phosphoheptose isomerase Putative glycosyl hydrolase Phosphohistidinoprotein-hexose...”
ETAE_2616 PTS system, N-acetylglucosamine-specific IIBC subunit from Edwardsiella tarda EIB202
44% identity, 8% coverage
Dred_0332 PTS system, glucose subfamily, IIA subunit from Desulfotomaculum reducens MI-1
38% identity, 11% coverage
ECA_RS21710 beta-glucoside-specific PTS transporter subunit IIABC from Pectobacterium atrosepticum SCRI1043
51% identity, 7% coverage
CAC3427 PTS system, (possibly glucose-specific) IIA component from Clostridium acetobutylicum ATCC 824
40% identity, 36% coverage
- Pleiotropic functions of catabolite control protein CcpA in Butanol-producing Clostridium acetobutylicum
Ren, BMC genomics 2012 - “...can directly repress them through binding to these CRE sites. Among these genes, CAC3425 and CAC3427 may deserve some attention. Although these two genes have been annotated as glucose-specific PTS components in the previous studies [ 37 - 39 ], they might be involved in transporting...”
- Metabolome remodeling during the acidogenic-solventogenic transition in Clostridium acetobutylicum
Amador-Noguez, Applied and environmental microbiology 2011 - “...phosphotransferase system (PTS), cac0570, cac2995, and cac3427; fumarate hydratase, cac3091; valine (Val), leucine (Leu), and isoleucine (Ile) biosynthesis,...”
- “...profile of glucose PTS genes (cac0570, cac2995, and cac3427) correlates with the drop in the levels of the upper glycolysis intermediates hexose-phosphate...”
ECs3289 glucose-specific PTS system IIA component from Escherichia coli O157:H7 str. Sakai
43% identity, 37% coverage
lp_3240 beta-glucosides PTS, EIIABC from Lactobacillus plantarum WCFS1
45% identity, 9% coverage
- Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study
Petrov, Genes 2021 - “...pts18CBA PTS transporter subunit EIIC NC +3.22 lp_0886, pts11BC PTS transporter subunit EIIC NC 3.91 lp_3240, pts28ABC PTS transporter subunit EIIC NC 2.19 lp_0436, pts7C PTS sugar transporter subunit IIC NC 17.13 lp_3507, pts29C PTS sugar transporter subunit IIC NC 6.13 lp_3010, pts23C PTS sugar transporter...”
- D-Alanylation of teichoic acids contributes to Lactobacillus plantarum-mediated Drosophila growth during chronic undernutrition
Matos, Nature microbiology 2017 - “...D-ala-D-ala carboxypeptidase ( pbpX2 ) 14-9 IG lp0616-lp0617 Intergenic region between secE and nusG 14-10 lp_3240 PTS system, beta-glucosides-specific EIIABC component ( pts28ABC ) 17-57 lp_2466 Prophage P2b protein 15, terminase large subunit 18-65 lp_1944 Multidrug ABC transporter 22-19 lp_2027 Chaperone, heat shock protein DnaK...”
YPTB1120 pts system, N-acetylglucosamine-specific IIABC component from Yersinia pseudotuberculosis IP 32953
42% identity, 8% coverage
lp_3240 glucose PTS transporter subunit IIA from Lactiplantibacillus plantarum WCFS1
45% identity, 9% coverage
- Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study
Petrov, Genes 2021 - “...pts18CBA PTS transporter subunit EIIC NC +3.22 lp_0886, pts11BC PTS transporter subunit EIIC NC 3.91 lp_3240, pts28ABC PTS transporter subunit EIIC NC 2.19 lp_0436, pts7C PTS sugar transporter subunit IIC NC 17.13 lp_3507, pts29C PTS sugar transporter subunit IIC NC 6.13 lp_3010, pts23C PTS sugar transporter...”
BSQ49_00775 sucrose-specific PTS transporter subunit IIBC from Liquorilactobacillus hordei
43% identity, 9% coverage
BB0559 PTS system, glucose-specific IIA component (crr) from Borrelia burgdorferi B31
35% identity, 33% coverage
- BosR and PlzA reciprocally regulate RpoS function to sustain Borrelia burgdorferi in ticks and mammals
Grassmann, The Journal of clinical investigation 2023 - “...43 ). Five genes ( bb0166 / malQ , bb0367 , bb0557 / ptsH-2 , bb0559 / crr , and bb0629 / fruA-2 ) encode components of the phosphoenolpyruvate-dependent sugar phosphotransferase system the spirochetes central pathway for carbohydrate transport ( 42 , 43 ) and could...”
- Global Tn-seq analysis of carbohydrate utilization and vertebrate infectivity of Borrelia burgdorferi
Troy, Molecular microbiology 2016 - “...presence of ten genes annotated to encode EII PTS transporter subunits ( bb0116, bb0367, bb0408, bb0559, bb0629, bb0645, bbb04, bbb05, bbb06, bbb29 ) suggests that this method of importing carbohydrates plays a significant role in the enzootic cycle. Transposon mutants with insertions in eight of these...”
- “...the EIIB and EIIC domains of a PTS transporter. It has been hypothesized that Crr (BB0559) is the EIIA component of these transporters. The fact that Crr may serve as the EIIA for multiple EIIBC transporters may explain why no transposon mutant in crr is present...”
- Stage-specific global alterations in the transcriptomes of Lyme disease spirochetes during tick feeding and following mammalian host adaptation
Iyer, Molecular microbiology 2015 - “...transporters or enzymes for carbohydrate or lipid metabolism (lactate dehydrogenase, acyl carrier protein, BB0557 [PtsH-2], BB0559 (glucose-specific EIIA PTS component), BBB29 [MalX2]) are also highly expressed. Table 2 lists the 100 most highly expressed genes in fed nymphs and, for comparison, also includes the relative expression...”
- “...of glucose, N-acetylglucosamine (GlcNAc), mannose, maltose, trehalose and chitobiose ( Figure 9A ). By homology, BB0559 (EIIA) and BB0645 (EIIBC) are annotated as glucose-specific, whereas BB0116 and BBB29 (EIIABC) are annotated as maltose- and glucose-specific ( Fraser et al. , 1997 ). Presumably, GlcNAc can also...”
- Borrelia burgdorferi requires glycerol for maximum fitness during the tick phase of the enzootic cycle
Pappas, PLoS pathogens 2011 - “...phosphorylated based on the phosphoenolpyruvate to pyruvate ratios. B. burgdorferi encodes a putative EIIA subunit (BB0559), but does not appear to contain other major components that modulate CCR in other bacteria [12] . It is reasonable to assume that B. burgdorferi possesses sensing mechanisms that monitor...”
- Profiling of temperature-induced changes in Borrelia burgdorferi gene expression by using whole genome arrays
Ojaimi, Infection and immunity 2003 - “...BB0049 BB0804 BB0233 BB0162 BB0500 BBA62 BB0232 BB0294 BB0559 BBA15 BB0300 BB0778 BBB19 BB0480 BB0486 BB0704 BB0365 BBA32 BBF20 BB0741 BBA16 BB0256 BBS37 BBQ42...”
- “...transcript level 23 35 BB0440 BBA59 BB0233 BB0049 BB0559 BB0804 BBA62 BB0300 BB0294 BB0500 BBA15 BB0232 BB0778 BB0047 BB0162 BB0486 BB0355 BBA16 BB0741 BB0480...”
- Global analysis of Borrelia burgdorferi genes regulated by mammalian host-specific signals
Brooks, Infection and immunity 2003 - “...BB0257 (cell division protein, putative [E. coli]) BB0559 (PTS system, glucose-specific IIA component [crr] [B. burgdorferi]) BB0764 (sensory transduction...”
BL1632 PtsG from Bifidobacterium longum NCC2705
41% identity, 7% coverage
- Preferential sugar utilization by bifidobacterial species
Murakami, Microbiome research reports 2023 - “...investigated the homolog distribution of the glcP , ptsG , and licT gene cluster (BL1631, BL1632, and BL1633 in B. longum NCC2705), which are reportedly involved in the preferential utilization of lactose in NCC2705 [ 20 ] . Of the 40 isolates, 12 strains had all...”
- Lactose-over-glucose preference in Bifidobacterium longum NCC2705: glcP, encoding a glucose transporter, is subject to lactose repression
Parche, Journal of bacteriology 2006 - “...BL1630-F1, TCAAGCGTGTGCCCTACGA BL1630-R1, GTGCTCGCGGAAGTCGAA BL1632 ( ptsG) AAN25420 BL1632-F1, CACCGACGCCGAACAAA BL1632-R1, GCGTGAAGTGACGGGATGTA BL1633...”
PMI2226 PTS system, IIabc component from Proteus mirabilis HI4320
47% identity, 8% coverage
- Twin arginine translocation, ammonia incorporation, and polyamine biosynthesis are crucial for Proteus mirabilis fitness during bloodstream infection
Armbruster, PLoS pathogens 2019 - “...factors in the spleen including, ptsG , ptsH , ptsI , treB , nagE , PMI2226, PMI2982, and PMI3515. Several genes involved in glycolysis were also identified as infection-specific fitness factors ( ptsG , gnd , edd , and pykA ), and genes involved in pyruvate...”
SG0859 PTS system N-acetylglucosamine-specific IIABC component from Sodalis glossinidius str. 'morsitans'
40% identity, 8% coverage
NH13_02050 sucrose-specific PTS transporter subunit IIBC from Lactobacillus acidophilus
51% identity, 8% coverage
- Draft Genome Sequence of the Probiotic Strain Lactobacillus acidophilus ATCC 4356
Palomino, Genome announcements 2015 - “...utilization functions were predicted by RASTtk, particularly the PTS system for N -acetylglucosamine-specific IIABC components (NH13_02050), as well as the N -acetyl- d -glucosamine ABC transport system, permease protein (NH13_08155). Mechanisms of the enzymes degrading N -acetylglucosamine ( 15 ) are predicted: NagB, GlcN6P deaminase (NH13_09645)...”
LBA1705 PTS system IIBC component from Lactobacillus acidophilus NCFM
42% identity, 10% coverage
ESA_02545 beta-glucoside-specific PTS transporter subunit IIABC from Cronobacter sakazakii ATCC BAA-894
ESA_02545 hypothetical protein from Enterobacter sakazakii ATCC BAA-894
42% identity, 10% coverage
- Comparative analysis of genome sequences covering the seven cronobacter species
Joseph, PloS one 2012 - “...involved in positive control of the utilization of different sugars by transcription antitermination [56] . ESA_02545 encodes a kinase which converts -glucosides to 6-phospho--glucosides and ESA_02546 encodes a 6-phospho--glucosidase specific to arbutin-6-phosphate and salicilin-6-phosphate. ESA_02547 also encodes 6-phospho--glucosidase, which may have the same or similar function...”
LBA0725 phosphotransferase system enzyme II from Lactobacillus acidophilus NCFM
40% identity, 9% coverage
- Microbial Unmasking of Plant Glycosides
Biernat, mBio 2018 - “...highly expressed upon exposure to all three plant glucosides and contained the phosphotransferase (PTS) transporter LBA0725 and the phospho--glucosidase (P-Bgl) LBA0726. A second gene locus containing a related but distinct PTS, LBA0227, and a second type of the P-Bgl, LBA0225, was upregulated following growth on amygdalin...”
- Lactobacillus acidophilus Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals
Theilmann, mBio 2017 - “...b Log 2 ratio c Amy/Glc Esc/Glc Sal/Glc LBA0227 PTS EIIC G 9.9 0.8 0.9 LBA0725 PTS EIIABC G 9.7 9.8 8.9 LBA0726 Phospho--glucosidase (GH1) G 7.2 6.9 6.2 LBA1436 Glycerol uptake facilitator protein G 7.2 4.9 3.8 LBA0631 Hypothetical protein 7.2 2.9 2.9 LBA1435 Hypothetical...”
- “...genes encode a LicT transcriptional antiterminator (LBA0724), an EIIABC component of a phosphotransferase system (PTS; LBA0725), a phospho--glucosidase (P-Bgl; LBA0726) of glycoside hydrolase family 1 (GH1) according to the CAZy database ( 34 ), and a hypothetical protein (LBA0728) ( Fig.2A ). These genes, except for...”
- The Differential Proteome of the Probiotic Lactobacillus acidophilus NCFM Grown on the Potential Prebiotic Cellobiose Shows Upregulation of Two β -Glycoside Hydrolases
van, BioMed research international 2015 - “...1 , spots 16 and 18) encoded in a gene cluster together with another PTS (LBA0725; Figure 3 ) indicating a role in cellobiose utilization ( Figure 3 ). This PTS LBA0725 has been reported to have 62% amino acid sequence identity to a PTS (ORF...”
- Transcriptional analysis of prebiotic uptake and catabolism by Lactobacillus acidophilus NCFM
Andersen, PloS one 2012 - “...including -1,4 or -1,6 glucoside specific PTS permeases, showed a clear segregation of LBA0227 and LBA0725, the latter clustering with a functionally characterized cellobiose PTS permease from L. gasseri ATCC 33323 [34] , consistent with the observed upregulation of LBA0725 on cellobiose. Notably, the PTS permease...”
- “...domains LBA0879 and LBA0884 were also upregulated by cellobiose, albeit at a lower level than LBA0725. These two proteins clustered distantly on the phylogenetic tree, indicating functional divergence and a likely preference for structurally-related substrates such as sophorose (- d -Glc p -(12)- d -Glc p...”
P43466 Raffinose carrier protein from Pediococcus pentosaceus
49% identity, 8% coverage
B1745_01765 sucrose-specific PTS transporter subunit IIBC from Lactobacillus amylolyticus
49% identity, 8% coverage
- Adaptive mechanism of Lactobacillus amylolyticus L6 in soymilk environment based on metabolism of nutrients and related gene-expression profiles
Fei, Food science & nutrition 2022 - “...metabolism were significantly upregulated in logarithmic phase, such as genes coding for PTS glucoside transporter (B1745_01765), 6phosphoalphaglucosidase (B1745_05130), gluconate kinase (B1745_01565), and glucose6phosphate dehydrogenase (B1745_01805) (Table 2 ). However, several genes involved in sucrose transportation, especially sugar ABC transporters (B1745_06760, B1745_06745 and B1745_06745), and galactose metabolism...”
- “...ldh Llactate dehydrogenase 3.18 B1745_05695 adhE acetaldehyde dehydrogenase /alcohol dehydrogenase 2.67 B1745_RS08070 galA alphagalactosidase 2.32 B1745_01765 scrA PTS betaglucoside transporter subunit EIIBCA 1.98 B1745_04615 spp HAD family hydrolase 1.93 B1745_03820 gyaR hypothetical protein 1.80 B1745_03825 spp sugarphosphatase 1.69 B1745_01805 zwf glucose6phosphate dehydrogenase 1.62 B1745_06170 gpmB histidine...”
Cbei_4533 PTS system, glucose subfamily, IIA subunit from Clostridium beijerincki NCIMB 8052
42% identity, 36% coverage
P45604 protein-Npi-phosphohistidine-N-acetyl-D-glucosamine phosphotransferase (EC 2.7.1.193) from Klebsiella pneumoniae (see paper)
42% identity, 8% coverage
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 798,070 different protein sequences to 1,261,478 scientific articles. Searches against EuropePMC were last performed on May 12 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory