PaperBLAST

Full List of Papers Linked to VIMSS10091231

PTR30_ARATH / Q8VZR7 Protein NRT1/ PTR FAMILY 5.1; AtNPF5.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
AT2G40460 proton-dependent oligopeptide transport (POT) family protein from Arabidopsis thaliana

• Arabidopsis NPF5.1 regulates ABA homeostasis and seed germination by mediating ABA uptake into the seed coat
  Shimizu, Plant signaling & behavior 2022
  • “...software (SCIEX). Results and discussions We previously reported that the transporter encoded by Arabidopsis NPF5.1 (At2g40460) mediates cellular ABA uptake. 8 NPF5.1 is expressed in vascular tissues and leaf mesophyll and epidermal cells during vegetative stages. Mutants defective in NPF5.1 ( npf5.1 ) exhibited higher leaf...”
• Genetic Determinants of Fiber-Associated Traits in Flax Identified by Omics Data Integration
  Kanapin, International journal of molecular sciences 2022
  • “...wall localized proteins tightly associated to its loosening and stiffening Lus10029063 major facilitator superfamily protein AT2G40460 [ 43 ] facilitates movement of small solutes across cell membranes Chr4:17106278 Lus10004043 myb domain protein 20 AT1G66230 [ 87 ] activates lignin and phenylalanine biosynthesis genes during secondary wall...”
• Genome-wide characterization, expression analyses, and functional prediction of the NPF family in Brassica napus
  Wen, BMC genomics 2020
  • “...NPF4 NO 3 [ 59 ]; ABA [ 17 , 18 , 58 ] AtNPF5.1 At2g40460 NPF51 ABA/GA 1/3/4 /MeJA [ 18 ] AtNPF5.2 PTR3 At5g46050 NPF51 ABA/GA 1/3/4 [ 9 ]; di-peptides [ 60 ] SA; MeJA; ABA AtNPF5.3 At5g46040 NPF51 ABA [ 18 ]...”
• Genome-wide identification, classification and transcriptional analysis of nitrate and ammonium transporters in Coffea
  Santos, Genetics and molecular biology 2017
  • “...was Cc02_g05650 , in agreement with the high expression profile of its ortholog oligopeptide transporter At2g40460 in Arabidopsis seeds ( von Wittgenstein et al ., 2014 ). These two genes are part of the super-group I, group I E ( Figure S2 ). The NRT2 family...”
• Regulation of transcription by the Arabidopsis UVR8 photoreceptor involves a specific histone modification
  Velanis, Plant molecular biology 2016
  • “...AT2G37970 SOUL heme-binding family protein Ch5 AT5G13930 CHS Chalcone and stilbene synthase family protein Chr2 AT2G40460 Major facilitator superfamily protein Ch5 AT5G17780 Alpha/beta-Hydrolases superfamily protein Chr3 AT3G10910 RING/U-box superfamily protein Ch5 AT5G19850 Alpha/beta-Hydrolases superfamily protein Chr3 AT3G14770 Nodulin MtN3 family protein Ch5 AT5G23730 RUP2 Transducin/WD40 repeat-like...”
• The circadian clock and defence signalling in plants
  Sharma, Molecular plant pathology 2015 (secret)
• Plant growth in Arabidopsis is assisted by compost soil-derived microbial communities
  Carvalhais, Frontiers in plant science 2013
  • “...(34.5%) 3815/29970 (12.7%) GT72B1, VTC2, RAP2.4, AT1G76190, CP12-2, HSF, A4A, AT1G70000, ATMRP7, CBL5, CRY1, AT2G31730, AT2G40460, STO, AT5G41750, AT3G23600, EBF1, PRXR1, RING1, YSL1 GO:0042221 response to chemical stimulus 4.36E-03 14/55 (25.5%) 1984/29970 (6.6%) GT72B1, VTC2, RAP2.4, CP12-2, HSF, A4A, AT1G70000, CBL5, AT2G31730, EBF1, PRXR1, RING1, YSL1...”
• Genomic survey, characterization and expression profile analysis of the peptide transporter family in rice (Oryza sativa L.)
  Zhao, BMC plant biology 2010
  • “...four pairs of orthologs (Os01g01360 and At5g13400, Os01g37590 and At2g26690, Os06g15370 and At1g68570, Os05g27010 and At2g40460) could be figured out. This result indicated that the main characteristics of PTR family in rice and Arabidopsis were formed before the split of monocotyledonous and dicotyledonous plants and then...”
  • “...and AtPTR genes showed the similar expression pattern such as Os06g15370 and At1g68570, Os05g27010 and At2g40460. They both had high expression level in all the tissues. However, no more apparent relationship between the expression pattern and sequence similarities of OsPTR and AtPTR members could be pointed...”
• The iron-responsive element (IRE)/iron-regulatory protein 1 (IRP1)-cytosolic aconitase iron-regulatory switch does not operate in plants
  Arnaud, The Biochemical journal 2007
  • “...and CAUUUU ACACAAUGC and CAUUUU At1g35510 At2g40460 At3g10220 At5g01450 At5g28150 At5g63780 Unknown Proton-dependent oligopeptide transporter Tubulin folding...”

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How It Works

PaperBLAST builds a database of protein sequences that are linked to scientific articles. These links come from automated text searches against the articles in EuropePMC and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot, BRENDA, CAZy (as made available by dbCAN), BioLiP, CharProtDB, MetaCyc, EcoCyc, TCDB, REBASE, the Fitness Browser, and a subset of the European Nucleotide Archive with the /experiment tag. Given this database and a protein sequence query, PaperBLAST uses protein-protein BLAST to find similar sequences with E < 0.001.

To build the database, we query EuropePMC with locus tags, with RefSeq protein identifiers, and with UniProt accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use queries of the form "locus_tag AND genus_name" to try to ensure that the paper is actually discussing that gene. Because EuropePMC indexes most recent biomedical papers, even if they are not open access, some of the links may be to papers that you cannot read or that our computers cannot read. We query each of these identifiers that appears in the open access part of EuropePMC, as well as every locus tag that appears in the 500 most-referenced genomes, so that a gene may appear in the PaperBLAST results even though none of the papers that mention it are open access. We also incorporate text-mined links from EuropePMC that link open access articles to UniProt or RefSeq identifiers. (This yields some additional links because EuropePMC uses different heuristics for their text mining than we do.)

For every article that mentions a locus tag, a RefSeq protein identifier, or a UniProt accession, we try to select one or two snippets of text that refer to the protein. If we cannot get access to the full text, we try to select a snippet from the abstract, but unfortunately, unique identifiers such as locus tags are rarely provided in abstracts.

PaperBLAST also incorporates manually-curated protein functions:

• Proteins from NCBI's RefSeq are included if a GeneRIF entry links the gene to an article in PubMed^®. GeneRIF also provides a short summary of the article's claim about the protein, which is shown instead of a snippet.
• Proteins from Swiss-Prot (the curated part of UniProt) are included if the curators identified experimental evidence for the protein's function (evidence code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that describe the protein's function are shown (with bold headings).
• Proteins from BRENDA, a curated database of enzymes, are included if they are linked to a paper in PubMed and their full sequence is known.
• Every protein from the non-redundant subset of BioLiP, a database of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself does not include descriptions of the proteins, those are taken from the Protein Data Bank. Descriptions from PDB rely on the original submitter of the structure and cannot be updated by others, so they may be less reliable. (For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every ligand is represented among a group of structures with similar sequences, but for PaperBLAST, we use the non-redundant set provided by BioLiP.)
• Every protein from EcoCyc, a curated database of the proteins in Escherichia coli K-12, is included, regardless of whether they are characterized or not.
• Proteins from the MetaCyc metabolic pathway database are included if they are linked to a paper in PubMed and their full sequence is known.
• Proteins from the Transport Classification Database (TCDB) are included if they have known substrate(s), have reference(s), and are not described as uncharacterized or putative. (Some of the references are not visible on the PaperBLAST web site.)
• Every protein from CharProtDB, a database of experimentally characterized protein annotations, is included.
• Proteins from the CAZy database of carbohydrate-active enzymes are included if they are associated with an Enzyme Classification number. Even though CAZy does not provide links from individual protein sequences to papers, these should all be experimentally-characterized proteins.
• Proteins from the REBASE database of restriction enzymes are included if they have known specificity.
• Every protein with an evidence-based reannotation (based on mutant phenotypes) in the Fitness Browser is included.
• Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators) with experimentally-determined DNA binding sites from the PRODORIC database of gene regulation in prokaryotes.
• Putative transcription factors from RegPrecise that have manually-curated predictions for their binding sites. These predictions are based on conserved putative regulatory sites across genomes that contain similar transcription factors, so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
• Coding sequence (CDS) features from the European Nucleotide Archive (ENA) are included if the /experiment tag is set (implying that there is experimental evidence for the annotation), the nucleotide entry links to paper(s) in PubMed, and the nucleotide entry is from the STD data class (implying that these are targeted annotated sequences, not from shotgun sequencing). Also, to filter out genes whose transcription or translation was detected, but whose function was not studied, nucleotide entries or papers with more than 25 such proteins are excluded. Descriptions from ENA rely on the original submitter of the sequence and cannot be updated by others, so they may be less reliable.

Except for GeneRIF and ENA, the curated entries include a short curated description of the protein's function. For entries from BioLiP, the protein's function may not be known beyond binding to the ligand. Many of these entries also link to articles in PubMed.

For more information see the PaperBLAST paper (mSystems 2017) or the code. You can download PaperBLAST's database here.

Changes to PaperBLAST since the paper was written:

• November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
• February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
• June 2022: incorporated some coding sequences from ENA with the /experiment tag.
• March 2022: incorporated BioLiP.
• April 2020: incorporated TCDB.
• April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
• February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
• January 2018: incorporated BRENDA.
• December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
• September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.

Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.

Secrets

PaperBLAST cannot provide snippets for many of the papers that are published in non-open-access journals. This limitation applies even if the paper is marked as "free" on the publisher's web site and is available in PubmedCentral or EuropePMC. If a journal that you publish in is marked as "secret," please consider publishing elsewhere.

Omissions from the PaperBLAST Database

Many important articles are missing from PaperBLAST, either because the article's full text is not in EuropePMC (as for many older articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an article that characterizes a protein's function but is missing from PaperBLAST, please notify the curators at UniProt or add an entry to GeneRIF. Entries in either of these databases will eventually be incorporated into PaperBLAST. Note that to add an entry to UniProt, you will need to find the UniProt identifier for the protein. If the protein is not already in UniProt, you can ask them to create an entry. To add an entry to GeneRIF, you will need an NCBI Gene identifier, but unfortunately many prokaryotic proteins in RefSeq do not have corresponding Gene identifers.

References

PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.

Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.

Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.

UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.

BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.

The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.

The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.

CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.

The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.

The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.

REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.

Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.