PaperBLAST
PaperBLAST Hits for BRENDA::H6LBB1 lactate dehydrogenase (NAD+, ferredoxin) (subunit 2/3) (EC 1.3.1.110) (Acetobacterium woodii) (418 a.a., MAGIKIIKEN...)
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>BRENDA::H6LBB1 lactate dehydrogenase (NAD+, ferredoxin) (subunit 2/3) (EC 1.3.1.110) (Acetobacterium woodii)
MAGIKIIKENVDRETFEALAEICPFDAFSYENDKLEVTAACKMCKMCLKKGPEGVLILEE
DEKVAIDKSLYRGITVYVDHIEGQIHPVTFELIGKARELAAVIGHPVYALLMGTNITEKA
DELLKYGVDKVFVYDKPELKHFVIEPYANVLEDFIEKVKPSSILVGATNVGRSLAPRVAA
RYRTGLTADCTILEMKENTDLVQIRPAFGGNIMAQIVTENTRPQFCTVRYKVFTAPERVN
EPWGDVEMMDIEKAKLVSAIEVMEVIKKEKGIDLSEAETIVAVGRGVKCEKDLDMIHEFA
EKIGATVACTRPGIEAGWFDARLQIGLSGRTVKPKLIIALGISGAVQFAAGMQNSEYIIA
INSDPKAPIFNIAHCGMVGDLYEILPELLTMIEGPENNKDTETISIPEAIETPERMVV
Running BLASTp...
Found 201 similar proteins in the literature:
LCTC_ACEWD / H6LBB1 Lactate dehydrogenase (NAD(+),ferredoxin) subunit LctC; Electron transfer flavoprotein large subunit; Electron transfer flavoprotein subunit alpha; EtfA; Lactate dehydrogenase-Etf complex subunit LctC; EC 1.1.1.436 from Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1) (see paper)
H6LBB1 lactate dehydrogenase (NAD+, ferredoxin) (subunit 2/3) (EC 1.3.1.110) from Acetobacterium woodii (see paper)
AWO_RS04415, Awo_c08720, WP_014355266 lactate dehydrogenase subunit LctC from Acetobacterium woodii DSM 1030
100% identity, 100% coverage
- function: The lactate dehydrogenase-Etf complex catalyzes the oxidation of lactate to pyruvate. It uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD(+) as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD(+). The electron transfer flavoprotein (Etf) mediates the electron transfer between the different donors and acceptors.
catalytic activity: lactate + 2 reduced [2Fe-2S]-[ferredoxin] + 2 NAD(+) = 2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + 2 NADH (RHEA:46964)
cofactor: FAD (Binds 1 FAD per subunit.)
cofactor: [4Fe-4S] cluster
subunit: Part of the stable heterotrimeric lactate dehydrogenase-Etf complex, which is formed by the lactate dehydrogenase LctD and the electron-transferring flavoprotein (Etf) alpha (LctC) and beta (LctB) subunits. - A new metabolic trait in an acetogen: Mixed acid fermentation of fructose in a methylene-tetrahydrofolate reductase mutant of Acetobacterium woodii
Moon, Environmental microbiology reports 2023 - “...metabolism 2799 2101 0.40 Awo_c08710 Electron transfer flavoprotein beta subunit Lactate metabolism 76,533 548 7.12 Awo_c08720 Electron transfer flavoprotein alpha subunit 123,511 505 7.93 Awo_c08730 Lactate dehydrogenase 226,503 653 8.44 Awo_c08740 Lactate permease 93,321 346 8.08 Awo_c08750 Lactate racemase 71,991 315 7.84 Awo_c12730 CxxC motifcontaining protein...”
- Bioinformatics and metabolic flux analysis highlight a new mechanism involved in lactate oxidation in Clostridium tyrobutyricum
Munier, International microbiology : the official journal of the Spanish Society for Microbiology 2023 - “...genes were performed on C. tyrobutyricum CIRM BIA 2237 (NCBI RefSeq: NZ_CP038158.1). The etfA (locus_tag: AWO_RS04415) and etfB (AWO_RS0441) genes of A. woodii DSM 1030 (NC_016894) (Weghoff et al. 2015 ) were used as queries. Searches, data analyses, and visualizations were performed with NCBI blast. The...”
- “...lactate racemase (EZN00_RS08610, CLJU_RS10605, and AWO_RS04430); EtfB (EZN00_RS08615, CLJU_RS10600, and AWO_RS04410); EtfA (EZN00_RS08620, CLJU_RS10595, and AWO_RS04415); LDH, lactate dehydrogenase (EZN00_RS08625, CLJU_RS10590, and AWO_RS04420) According to these results, C.tyrobutyricum would have the same mechanism of lactate oxidation as A. woodii , i.e., involving an EtfAB complex. In...”
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...Clostridium butyricum KNU-L09 (NCBI RefSeq: NZ_CP013252, NZ_CP013489) with a custom made database. The etfA (locus_tag: AWO_RS04415) and etfB (AWO_RS0441) genes of Acetobacterium woodii DSM 1030 (NC_016894) [ 20 ] were used as queries. Searches, data analysis and visualisations were performed with Geneious 10.2.4 [ 27 ]....”
- “...and tBLASTn [ 29 ] searches were performed with the A. woodii DSM 1030 EtfA (AWO_RS04415) and EtfB (AWO_RS04410), with default parameters (Matrix: Blosum62, Gap cost: 11, Gap extend: 1, Word size: 6). The selected EtfA and EtfB proteins were subjected to phylogenetic analysis. The particular...”
- Methane-yielding microbial communities processing lactate-rich substrates: a piece of the anaerobic digestion puzzle
Detman, Biotechnology for biofuels 2018 - “...permease WP_014355268 (AWO_RS04425) (LldP), lactate racemase WP_014355269 (AWO_RS04430) (LarA), electron transfer flavoprotein subunit alpha WP_014355266 (AWO_RS04415) (EtfA), FAD/FMN-containing dehydrogenase WP_014355267 (AWO_RS04420) (GlcD), electron transporter RnfC WP_014356580 (AWO_RS11370) all from Acetobacterium woodii DSM 1030 genome NC_016894; l -lactate utilization protein LutB containing FeS oxidoreductase WP_028317114 (Q362_RS0100810) from...”
- “...lactate permease WP_014355268 (AWO_RS04425) (LldP), lactate racemase WP_014355269 (AWO_RS04430) (LarA), electron transfer flavoprotein subunit alpha WP_014355266 (AWO_RS04415) (EtfA), FAD/FMN-containing dehydrogenase WP_014355267 (AWO_RS04420) (GlcD), electron transporter RnfC WP_014356580 (AWO_RS11370) all from Acetobacterium woodii DSM 1030 genome NC_016894; l -lactate utilization protein LutB containing FeS oxidoreductase WP_028317114 (Q362_RS0100810)...”
7qh2A / H6LBB1 Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
100% identity, 81% coverage
- Ligand: flavin-adenine dinucleotide (7qh2A)
CPZ25_RS02225 lactate dehydrogenase subunit LctC from Eubacterium maltosivorans
74% identity, 96% coverage
EZN00_RS08620 electron transfer flavoprotein subunit alpha from Clostridium tyrobutyricum
64% identity, 95% coverage
- Bioinformatics and metabolic flux analysis highlight a new mechanism involved in lactate oxidation in Clostridium tyrobutyricum
Munier, International microbiology : the official journal of the Spanish Society for Microbiology 2023 - “...oxidoreductase genes. EtfB (1: EZN00_RS10860, 2: EZN00_RS10795, 3: EZN00_RS08615), EtfA (1: EZN00_RS10865, 2: EZN00_RS10800, 3: EZN00_RS08620), FAD-binding oxidoreductase (1:: EZN00_RS10870; 3: EZN00_RS08625) In fragment 1, genes encoding the EtfAB complex are next to a FAD-binding oxidoreductase. The EtfAB complex involved in fragment 2 is in a...”
- “...CLJU_RS10610, and AWO_RS04425); lactate racemase (EZN00_RS08610, CLJU_RS10605, and AWO_RS04430); EtfB (EZN00_RS08615, CLJU_RS10600, and AWO_RS04410); EtfA (EZN00_RS08620, CLJU_RS10595, and AWO_RS04415); LDH, lactate dehydrogenase (EZN00_RS08625, CLJU_RS10590, and AWO_RS04420) According to these results, C.tyrobutyricum would have the same mechanism of lactate oxidation as A. woodii , i.e., involving an...”
CCS79_RS18270, X276_25270 electron transfer flavoprotein subunit alpha from Clostridium beijerinckii NRRL B-598
65% identity, 90% coverage
- Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level
Patakova, Scientific reports 2019 - “...had putative heterodimer structures, composed of Etf subunits alpha and beta. The Etf system (X276_25275, X276_25270) that might be involved in DHDPA oxidation was chosen based on increased transcription observed during the T4 T6 time interval, see Fig. 7B . In addition to SSPs and dipicolinic...”
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24810), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2709), C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04240), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24820), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2710), C_acetobutylicum_ATTC824_GlcD (CA_C2544),...”
Cbei_0311 electron transfer flavoprotein, alpha subunit-like protein from Clostridium beijerincki NCIMB 8052
64% identity, 90% coverage
- Sigma Factor Regulated Cellular Response in a Non-solvent Producing Clostridium beijerinckii Degenerated Strain: A Comparative Transcriptome Analysis
Zhang, Frontiers in microbiology 2017 - “...accession number GSE63671). Quantitative Real-Time PCR (qRT-PCR) To validate microarray data, 10 up-regulated (Cbei_4123, Cbei_0411, Cbei_0311, Cbei_3120, Cbei_0441, Cbei_0331, Cbei_4824, Cbei_2740, Cbei_4871, and Cbei_3356) and 10 down-regulated (Cbei_3835, Cbei_1930, Cbei_0677, Cbei_2826, Cbei_2600, Cbei_1583, Cbei_1079, Cbei_0284, Cbei_3832, and Cbei_2261) genes were randomly selected for qRT-PCR. C. beijerinckii...”
- Transcriptional analysis of degenerate strain Clostridium beijerinckii DG-8052 reveals a pleiotropic response to CaCO3-associated recovery of solvent production
Jiao, Scientific reports 2016 - “...accession number of GSE81808. Real-time quantitative PCR (qRT-PCR) Following microarray analysis results, 10 up-regulated genes (Cbei_0311, Cbei_2826, Cbei_0795, Cbei_0870, Cbei_1113, Cbei_3472, Cbei_1108, Cbei_1255, Cbei_4644, Cbei_1130) and 10 down-regulated genes (Cbei_4123, Cbei_0331, Cbei_4871, Cbei_1221, Cbei_4669, Cbei_0411, Cbei_2261, Cbei_1227, Cbei_4406, Cbei_4491) were selected to validate microarray by q-RT-PCR....”
CLJU_RS10595 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium ljungdahlii DSM 13528
64% identity, 94% coverage
- Bioinformatics and metabolic flux analysis highlight a new mechanism involved in lactate oxidation in Clostridium tyrobutyricum
Munier, International microbiology : the official journal of the Spanish Society for Microbiology 2023 - “...and AWO_RS04425); lactate racemase (EZN00_RS08610, CLJU_RS10605, and AWO_RS04430); EtfB (EZN00_RS08615, CLJU_RS10600, and AWO_RS04410); EtfA (EZN00_RS08620, CLJU_RS10595, and AWO_RS04415); LDH, lactate dehydrogenase (EZN00_RS08625, CLJU_RS10590, and AWO_RS04420) According to these results, C.tyrobutyricum would have the same mechanism of lactate oxidation as A. woodii , i.e., involving an EtfAB...”
- Transcriptomic profiles of Clostridium ljungdahlii during lithotrophic growth with syngas or H2 and CO2 compared to organotrophic growth with fructose
Aklujkar, Scientific reports 2017 - “...Lactobacillus plantarum 29 , respectively. The ninth, tenth and thirteenth most upregulated genes ( lutJ CLJU_RS10595, lutI CLJU_RS10600 and lutK CLJU_RS10590, Fig. 1 ) encode an electron transfer flavoprotein and a candidate (R) -lactate dehydrogenase with 28% protein sequence identity to an (R) -lactate-oxidizing enzyme of...”
CA_C2543 FAD-binding protein from Clostridium acetobutylicum ATCC 824
CAC2543 Electron-transferring flavoprotein large subunit from Clostridium acetobutylicum ATCC 824
62% identity, 94% coverage
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...A_woodii_DSM1030_acyl-CoA (AWO_RS08105), A_woodii_DSM1030_GlcD_LldP (AWO_RS04415), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07090), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04260), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04230), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24810), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2709), C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100),...”
- Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in Clostridium acetobutylicum EA 2018
Hu, BMC genomics 2011 - “...2.50221 2.005231 Fe-S oxidoreductase CEA_G2555 CA_C2542 8.22595 5.876659 5.423124 5.135223 4.224852 4.280766 FAD/FMN-containing dehydrogenase CEA_G2556 CA_C2543 8.451735 6.100494 5.401602 5.24343 4.426238 3.987954 electron-transferring flavoprotein large subunit CEA_G2557 CA_C2544 7.904188 6.040402 4.848558 4.578106 4.214698 3.969443 electron-transferring flavoprotein small subunit CEA_G3411 CA_C3408 -2.71993 -3.64684 -4.53359 -4.7967 -4.89809 -4.45863...”
- Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in Clostridium acetobutylicum EA 2018
Hu, BMC genomics 2011 - “...Predicted flavoprotein CEA_G2485 CAC2471 439(A) 438-439(-) 147-150(R,L,P,I) 147-150 (G,C,L,Stop codon) Transcriptional regulator, TetR/AcrR family CEA_G2556 CAC2543 241-249 (G,T,A,G, A,T,C,A,T) 240-241(---) 81-83(V,D,H) 80-81(---) Electron-transferring flavoprotein large subunit CEA_G2806 CAC2798 286(A) 286(G) 96(M) 96(V) NADH:flavin oxidoreductase CEA_P0058 CA_P0059 754(A) 754(C) 252(N) 252(H) Alcohol dehydrogenase CEA_P0077 CA_P0078 91(A) 91(G)...”
CEA_G2556 FAD-binding protein from Clostridium acetobutylicum EA 2018
62% identity, 94% coverage
- Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in Clostridium acetobutylicum EA 2018
Hu, BMC genomics 2011 - “...86(G) Predicted flavoprotein CEA_G2485 CAC2471 439(A) 438-439(-) 147-150(R,L,P,I) 147-150 (G,C,L,Stop codon) Transcriptional regulator, TetR/AcrR family CEA_G2556 CAC2543 241-249 (G,T,A,G, A,T,C,A,T) 240-241(---) 81-83(V,D,H) 80-81(---) Electron-transferring flavoprotein large subunit CEA_G2806 CAC2798 286(A) 286(G) 96(M) 96(V) NADH:flavin oxidoreductase CEA_P0058 CA_P0059 754(A) 754(C) 252(N) 252(H) Alcohol dehydrogenase CEA_P0077 CA_P0078 91(A)...”
- “...3.003615 2.50221 2.005231 Fe-S oxidoreductase CEA_G2555 CA_C2542 8.22595 5.876659 5.423124 5.135223 4.224852 4.280766 FAD/FMN-containing dehydrogenase CEA_G2556 CA_C2543 8.451735 6.100494 5.401602 5.24343 4.426238 3.987954 electron-transferring flavoprotein large subunit CEA_G2557 CA_C2544 7.904188 6.040402 4.848558 4.578106 4.214698 3.969443 electron-transferring flavoprotein small subunit CEA_G3411 CA_C3408 -2.71993 -3.64684 -4.53359 -4.7967 -4.89809...”
ATN24_RS03030 FAD-binding protein from Clostridium butyricum
63% identity, 90% coverage
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...B_methylotrophicum_DSM3468_acyl-CoA (BUME_07090), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04260), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04230), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24810), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2709), C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04240),...”
- “...Four fragments of the NZ_CP013252 chromosome of C. butyricum KNU-L09 containing genes encoding EtfA (1: ATN24_RS03030, 2: ATN24_RS03165, 3: ATN24_RS08885) and EtfB (1: ATN24_RS03025, 2: ATN24_RS03160, 3: ATN24_RS08880), and potentially GlcD (1: ATN24_RS03035, 3: ATN24_RS08895, 4: ATN24_RS11095) A similar search was performed for the selected genomes...”
CPE0312 electron-transfer flavoprotein alpha-subunit from Clostridium perfringens str. 13
62% identity, 91% coverage
CD630_11720, CDIF630erm_01320 FAD-binding protein from Clostridioides difficile 630
56% identity, 94% coverage
- The WalRK Two-Component System Is Essential for Proper Cell Envelope Biogenesis in Clostridioides difficile
Müh, Journal of bacteriology 2022 - “...Hypothetical protein 3.52 1.83 NC cd630_11710 11710 etfB4 Electron transfer flavoprotein alpha 3.30 1.40 NC cd630_11720 11720 etfA4 Electron transfer flavoprotein alpha 2.58 1.24 NC cd630_11730 11730 FAD-linked oxidase 2.17 1.08 NC cd630_08320 08320 aksA trans -Homoaconitate synthase 3.11 2.02 NC cd630_08330 08330 acnB Aconitate hydratase...”
- Iron Regulation in Clostridioides difficile
Berges, Frontiers in microbiology 2018 - “...2.86 CD630_11710 CDIF630erm_01319 etfB Lactate dehydrogenase, electron transfer flavoprotein beta subunit 0.67 OFF 2.43 OFF CD630_11720 CDIF630erm_01320 etfA Lactate dehydrogenase, electron transfer flavoprotein alpha subunit -0.66 0.63 CD630_11730 CDIF630erm_01321 lactate dehydrogenase (electron bifurcating), catalytic subunit -0.30 -0.57 0.73 OFF CD630_03940 CDIF630erm_00522 ldhA (R)-2-hydroxyisocaproate dehydrogenase -8.37 OFF...”
- “...CD630_11710 CDIF630erm_01319 etfB Lactate dehydrogenase, electron transfer flavoprotein beta subunit 0.67 OFF 2.43 OFF CD630_11720 CDIF630erm_01320 etfA Lactate dehydrogenase, electron transfer flavoprotein alpha subunit -0.66 0.63 CD630_11730 CDIF630erm_01321 lactate dehydrogenase (electron bifurcating), catalytic subunit -0.30 -0.57 0.73 OFF CD630_03940 CDIF630erm_00522 ldhA (R)-2-hydroxyisocaproate dehydrogenase -8.37 OFF -8.66...”
WP_069871749 electron transfer flavoprotein subunit alpha/FixB family protein from Fusibacter sp. 3D3
55% identity, 94% coverage
- Looking for the mechanism of arsenate respiration of Fusibacter sp. strain 3D3, independent of ArrAB
Acosta-Grinok, Frontiers in microbiology 2022 - “...dehydrogenase (NAD + , ferredoxin) WP_069875591 Clostridium acetobutylicum P52042 0 EtfA-1 Electron transfer flavoprotein, subunit WP_069871749 Clostridium saccharobutylicum P53578 5 10 128 EtfB-1 Electron transfer flavoprotein, subunit WP_069871747 Clostridium amylolyticum A0A1M6NXL2 2 10 133 LdhD Lactate/Glycolate dehydrogenase, subunit LdhD/GlcD WP_069871751 Caldisalinibacter kiritimatiensis R1AW66 0 PFOR: pyruvate:ferredoxin...”
CARE_ACEWD / H6LGM8 Caffeyl-CoA reductase-Etf complex subunit CarE; Electron transfer flavoprotein large subunit; ETFLS; Electron transfer flavoprotein subunit alpha; Alpha-ETF; EC 1.3.1.108 from Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1) (see paper)
H6LGM8 caffeoyl-CoA reductase (subunit 1/3) (EC 1.3.1.108) from Acetobacterium woodii (see paper)
AWO_RS08105, Awo_c15740 caffeyl-CoA reductase-Etf complex subunit CarE from Acetobacterium woodii DSM 1030
41% identity, 88% coverage
- function: Caffeyl-CoA reductase-Etf complex catalyzes the reduction of caffeyl-CoA to yield hydrocaffeyl-CoA. It couples the endergonic ferredoxin reduction with NADH as reductant to the exergonic reduction of caffeoyl-CoA with the same reductant. It uses the mechanism of electron bifurcation to overcome the steep energy barrier in ferredoxin reduction. The electron transfer flavoprotein (Etf) mediates the electron transfer between the different donors and acceptors. The iron- sulfur cluster may be involved in electron transport, possibly in the intramolecular electron transfer from the Etf protein subunit to the caffeyl-CoA reductase subunit inside the complex. The complex can also reduce 4-coumaroyl-CoA and feruloyl-CoA.
catalytic activity: hydrocaffeoyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 NAD(+) = (E)-caffeoyl-CoA + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 NADH (RHEA:46956)
cofactor: FAD (Binds 1 FAD per subunit.)
cofactor: [4Fe-4S] cluster (Binds 2 [4Fe-4S] cluster per subunit.)
subunit: Part of the homotrimeric caffeyl-CoA reductase-Etf complex composed of (R)-2-hydroxyisocaproyl-CoA dehydratase CarC, and the electron transfer flavoprotein (ETF) alpha (CarE) and beta (CarD) subunits. - A new metabolic trait in an acetogen: Mixed acid fermentation of fructose in a methylene-tetrahydrofolate reductase mutant of Acetobacterium woodii
Moon, Environmental microbiology reports 2023 - “...dehydrogenase CarC 1006 59 4.09 Awo_c15730 Electron transfer flavoprotein beta subunit CarD 1220 52 4.55 Awo_c15740 Electron transfer flavoprotein alpha subunit apoprotein CarE 2209 125 4.14 Awo_c24340 Alanine or glycine:cation symporter Alanine metabolism 453 123 1.84 Awo_c24350 Alanine dehydrogenase 9213 863 3.39 Awo_c24360 AsnC family transcriptional...”
- Defining Genomic and Predicted Metabolic Features of the Acetobacterium Genus
Ross, mSystems 2020 - “...is encoded by carA2 , carB2 , carC , carD , and carE (Awo_c15700 to Awo_c15740). Specifically, CarA is a hydrocaffeyl-CoA:caffeate CoA transferase ( 25 ), CarB is an ATP-dependent acyl-CoA synthetase ( 84 ), CarC is a caffeyl-CoA reductase, and CarDE is an electron transfer...”
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...3 ) according to the genetic context in which they were found. For EtfA: A_woodii_DSM1030_acyl-CoA (AWO_RS08105), A_woodii_DSM1030_GlcD_LldP (AWO_RS04415), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07090), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04260), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04230), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24810), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2709), C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115),...”
6fahE / H6LGM8 Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
41% identity, 88% coverage
- Ligands: flavin-adenine dinucleotide; iron/sulfur cluster (6fahE)
Sfum_1373 electron transfer flavoprotein, alpha subunit from Syntrophobacter fumaroxidans MPOB
41% identity, 89% coverage
Ccar_RS01430 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium carboxidivorans P7
45% identity, 78% coverage
F502_06282 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium pasteurianum DSM 525 = ATCC 6013
44% identity, 77% coverage
- Metabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability
Groeger, Microbial cell factories 2017 - “...IscU 519 0.4 2.4 0.6 1.6 1.3 0.8 0.9 1.1 520 3.7 2.9 1.9 1.5 F502_06282 Electron transfer flavoprotein subunit alpha COG2025 FixB 440 1.5 F502_06287 Electron transfer flavoprotein subunit alpha/beta-like protein COG2086 FixA 487 1.7 F502_06447 Bifunctional acetaldehyde-CoA/alcohol dehydrogenase COG1012 AdhE 119 2.1 3.7 1.6...”
- “...[ 28 , 37 ]. In this proteomic study, two ETFs, namely ETFs subunit alpha (F502_06282) and subunit alpha/beta-like protein (F502_06287), were identified among the most abundant proteins regardless of the iron availability; however, their abundances were slightly higher (1.51.7 folds) in the late phase of...”
E3HC30 Electron transfer flavoprotein alpha/beta-subunit from Ilyobacter polytropus (strain ATCC 51220 / DSM 2926 / LMG 16218 / CuHBu1)
46% identity, 76% coverage
CAC2709 Electron transfer flavoprotein alpha-subunit from Clostridium acetobutylicum ATCC 824
CA_C2709 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium acetobutylicum ATCC 824
43% identity, 79% coverage
- Transcriptional analysis of micronutrient zinc-associated response for enhanced carbohydrate utilization and earlier solventogenesis in Clostridium acetobutylicum
Wu, Scientific reports 2015 - “...of the expression of the pyruvate to butyryl-CoA formation genes, thlA (CAC2873), crt (CAC2712), etfA (CAC2709), etfB (CAC2710), and bcd (CAC2711) were differentially upregulated by no more than 1.75-fold. Surprisingly, thlB (CAC0078) encoding acetyl-CoA acetyltransferase was 0.65-fold downregulated compared to 1.46-fold upregulation of the primary thiolase...”
- Complex and extensive post-transcriptional regulation revealed by integrative proteomic and transcriptomic analysis of metabolite stress response in Clostridium acetobutylicum
Venkataramanan, Biotechnology for biofuels 2015 - “...butanol stress, three (CAC2229 - pyruvate:ferredoxin oxidoreductase, CAC0578 - MetH, CAC3392 - Bdh) and five (CAC2709 - EtfA, CAC0022 - aspartate semialdehyde dehydrogenase, CAP0165 - Adc, CAC2333 - SpsI, CAC3146 - RplJ) were identified under medium and high stress, respectively, while four (CAC3171, CAC3598, CAC0116 -...”
- Redox-responsive repressor Rex modulates alcohol production and oxidative stress tolerance in Clostridium acetobutylicum
Zhang, Journal of bacteriology 2014 - “...asrC CAP0035 CAC0267 CAC2873 CAC2712 CAC2711 CAC2710 CAC2709 CAC2708 CAC3076 CAC3075 CAC1025 CAC1024 CAC1023 CAC1512 CAC1513 CAC1514 CAC1515 164.75 6.45 13.75...”
- A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat culture
Janssen, Applied microbiology and biotechnology 2010 - “...3.1 1.5 E CAC2702 Possible signal transduction protein 4.0 3.7 3.6 5.0 4.1 0.6 T CAC2709 etfA Electron transfer flavoprotein alpha-subunit 2.3 2.0 5.4 2.4 3.0 1.6 C CAC2711 bcd Butyryl-CoA dehydrogenase 2.0 1.8 6.1 2.4 3.1 2.0 I CAC2712 crt Enoyl-CoA hydratase 2.1 1.8 7.2...”
- “...( bcd , CAC2711), and the subunit of the electron transfer flavoprotein ( etfA , CAC2709). Otherwise, 3-hydroxybutyryl-CoA dehydrogenase ( hbd , CAC2708) and the second subunit of the electron transfer flavoprotein ( etfB , CAC2710) did not show significantly elevated transcript levels (SD1, TableS 2...”
- The role of PerR in O2-affected gene expression of Clostridium acetobutylicum
Hillmann, Journal of bacteriology 2009 - “...CAC0712 CAC0713 CAC2452 CAC2458 CAC2459 CAC2499 CAC2708 CAC2709 CAC2710 CAC2711 CAC2712 CAC2873 CAC3075 CAC3076 CAC3657 CAC3658 CAC3659 Arginine biosynthesis...”
- Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum
Alsaker, Journal of bacteriology 2005 - “...genes thl (CAC2873), hbd (CAC2708), crt (CAC2712), etfA (CAC2709), etfB (CAC2710), and bcd (CAC2711) generally increased in stationary phase but not more than...”
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...For EtfA: A_woodii_DSM1030_acyl-CoA (AWO_RS08105), A_woodii_DSM1030_GlcD_LldP (AWO_RS04415), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07090), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04260), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04230), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24810), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2709), C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA...”
- Quantitative proteomic analysis of the influence of lignin on biofuel production by Clostridium acetobutylicum ATCC 824
Raut, Biotechnology for biofuels 2016 - “...[ 68 ]. Interestingly, we identified butyryl-CoA dehydrogenase (Bcd) (CA_C2711), electron transfer flavoprotein (subunit etfA (CA_C2709) and etfB (CA_C2710) and probable NADH/NADPH oxidoreductase (CA_C1958) in both conditions, suggesting the presence of extra energy conserving modules in this bacterium. Conclusions In conclusion, our study presents the most...”
- A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum
Yoo, mBio 2015 - “...The previously uncharacterized butyryl coenzyme A (butyryl-CoA) dehydrogenase (BCD) encoded by bcd-etfB-etfA (CA_C2711, CA_C2710, and CA_C2709, respectively) ( 42 ) was biochemically characterized via homologous expression of the encoding operon in C.acetobutylicum and the purification of the enzyme complex ( Table2 ; see also Fig.S1 )....”
- “...reaction: the BCD complex encoded by bcd , etfB , and etfA (CA_C2711, CA_C2710, and CA_C2709, respectively) which consumes 2moles of NADH and produces 1mole of reduced ferredoxin (see Fig.S1 in the supplemental material) and TER ( trans -2-enoyl-CoA reductase) encoded by CA_C0642, which consumes only...”
- Converting carbon dioxide to butyrate with an engineered strain of Clostridium ljungdahlii
Ueki, mBio 2014 - “...crt (NCBI GenBank; CA_C2712), bcd (NCBI GenBank; CA_C2711), etfB (NCBI GenBank, CA_C2710), etfA (NCBI GenBank; CA_C2709), hbd (NCBI GenBank; CA_C2708), buk (NCBI GenBank; CA_C3075), and ptb (NCBI GenBank; CA_C3076). Primers used for the PCR are listed in TableS1 in the supplemental material. PCR products were cloned...”
CKL_RS02260 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium kluyveri DSM 555
44% identity, 77% coverage
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04240), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24820), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2710), C_acetobutylicum_ATTC824_GlcD (CA_C2544), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03160), C_butyricum_KNU-L09_GlcD (ATN24_RS03025),...”
P53578 Protein FixB from Clostridium saccharobutylicum
45% identity, 76% coverage
- Looking for the mechanism of arsenate respiration of Fusibacter sp. strain 3D3, independent of ArrAB
Acosta-Grinok, Frontiers in microbiology 2022 - “..., ferredoxin) WP_069875591 Clostridium acetobutylicum P52042 0 EtfA-1 Electron transfer flavoprotein, subunit WP_069871749 Clostridium saccharobutylicum P53578 5 10 128 EtfB-1 Electron transfer flavoprotein, subunit WP_069871747 Clostridium amylolyticum A0A1M6NXL2 2 10 133 LdhD Lactate/Glycolate dehydrogenase, subunit LdhD/GlcD WP_069871751 Caldisalinibacter kiritimatiensis R1AW66 0 PFOR: pyruvate:ferredoxin oxidoreductase PorA-1 Pyruvate...”
Cbei_0324 electron transfer flavoprotein, alpha/beta-subunit-like protein from Clostridium beijerincki NCIMB 8052
43% identity, 77% coverage
- Model-driven approach for the production of butyrate from CO2/H2 by a novel co-culture of C. autoethanogenum and C. beijerinckii
Benito-Vaquerizo, Frontiers in microbiology 2022 - “...reduced ferredoxin. Two complete clusters were identified in the genome: Cbei_0322 (Bcd), Cbei_0323 (EtfB), and Cbei_0324 (EtfA) or Cbei_2035 (Bcd), Cbei_2036 (EtfB) and Cbei_2037 (EtfA). An acyl-CoA dehydrogenase (Acd) showed 79.4% similarity with the Bcd subunit of C. acetobutylicum ATCC 824. Butyrate can be produced from...”
- Sigma Factor Regulated Cellular Response in a Non-solvent Producing Clostridium beijerinckii Degenerated Strain: A Comparative Transcriptome Analysis
Zhang, Frontiers in microbiology 2017 - “...WT-8052 in enzymes, which including acetyl-CoA acetyltransferase (Cbei_0411, Cbei_3630) catalyzing acetyl-CoA into acetoacetyl-CoA; 3-hydroxybutyryl-CoA dehydrogenase (Cbei_0324, Cbei_0325, Cbei_2037) catalyzing acetoacetyl-CoA into 3-hydroxybutyryl-CoA; Enoyl-CoA hydratase (Cbei_2231, Cbei_2230) catalyzing 3-hydroxybutyryl-CoA into crotonyl-CoA; butyryl-CoA dehydrogenase (Cbei_0322, Cbei_2035, Cbei_2883) catalyzing crotonyl-CoA into butyryl-CoA ( Figures 3D and 4C ; Supplementary...”
- Transcriptional analysis of degenerate strain Clostridium beijerinckii DG-8052 reveals a pleiotropic response to CaCO3-associated recovery of solvent production
Jiao, Scientific reports 2016 - “...may cause the different sensitivity to CaCO 3. The transcription of 3-hydroxybutyryl-CoA dehydrogenase (Cbei_0325), 3-hydroxybutyryl-CoA (Cbei_0324) and butyryl-CoA dehydrogenase (Cbei_0322) was not changed significantly by the addition of CaCO 3 to the DG-8052 culture. It is reported that phosphoenolpyruvate (PEP), pyruvate, and acetyl-CoA form three main...”
CCS79_RS24290 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium diolis
43% identity, 77% coverage
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...B_methylotrophicum_DSM3468_GlcD_2 (BUME_04230), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24810), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2709), C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04240), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24820), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2710),...”
CKL_RS17115 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium kluyveri DSM 555
45% identity, 77% coverage
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04240), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24820), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2710), C_acetobutylicum_ATTC824_GlcD (CA_C2544), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03160), C_butyricum_KNU-L09_GlcD (ATN24_RS03025), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08880),...”
Ccar_22805, Ccar_RS22800 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium carboxidivorans P7
44% identity, 78% coverage
- Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from CO2 and H2
Lauer, Microbial cell factories 2022 - “...biosynthesis. Two homologous sets of these genes are present in the C. carboxidivorans genome: Ccar_RS22775 Ccar_RS22800 (Ccar1) and Ccar_RS01400 Ccar_RS01430 (Ccar2). We therefore amplified them from genomic DNA and placed them under the control of the C. acetobutylicum constitutive thlA promoter or the C. ljungdahlii constitutive...”
- Combination of Trace Metal to Improve Solventogenesis of Clostridium carboxidivorans P7 in Syngas Fermentation
Han, Frontiers in microbiology 2020 - “...crt Crotonase Ccar_22785 hbd 3-Hydroxybutyryl-CoA dehydrogenase Ccar_22790 thl Acetyl-CoA acetyltransferase Ccar_22800 etfB Electron transfer flavoprotein Ccar_22805 etfA Electron transfer flavoprotein subunit alpha Ccar_00690 pta Phosphate acetyltransferase Acetate synthesis from acetyl-CoA Ccar_00695 ack Acetate kinase Ccar_19520 ptb Phosphate butyryltransferase Butyrate synthesis from butyral-CoA Ccar_19515 buk Butyrate kinase...”
- “...and etfB . These six genes were clustered together on the chromosome from Ccar_22780 to Ccar_22805 and were all dramatically up-regulated under the Mo (0x) condition compared to levels observed in the Mo (1x) condition ( Figure 5B ). Two adjacent CDSs (Ccar_19515 and Ccar_19520) that...”
Cbei_2037 electron transfer flavoprotein, alpha/beta-subunit-like protein from Clostridium beijerincki NCIMB 8052
43% identity, 77% coverage
- Model-driven approach for the production of butyrate from CO2/H2 by a novel co-culture of C. autoethanogenum and C. beijerinckii
Benito-Vaquerizo, Frontiers in microbiology 2022 - “...the genome: Cbei_0322 (Bcd), Cbei_0323 (EtfB), and Cbei_0324 (EtfA) or Cbei_2035 (Bcd), Cbei_2036 (EtfB) and Cbei_2037 (EtfA). An acyl-CoA dehydrogenase (Acd) showed 79.4% similarity with the Bcd subunit of C. acetobutylicum ATCC 824. Butyrate can be produced from butyryl-CoA via two routes in C. beijerinckii ....”
- Sigma Factor Regulated Cellular Response in a Non-solvent Producing Clostridium beijerinckii Degenerated Strain: A Comparative Transcriptome Analysis
Zhang, Frontiers in microbiology 2017 - “...enzymes, which including acetyl-CoA acetyltransferase (Cbei_0411, Cbei_3630) catalyzing acetyl-CoA into acetoacetyl-CoA; 3-hydroxybutyryl-CoA dehydrogenase (Cbei_0324, Cbei_0325, Cbei_2037) catalyzing acetoacetyl-CoA into 3-hydroxybutyryl-CoA; Enoyl-CoA hydratase (Cbei_2231, Cbei_2230) catalyzing 3-hydroxybutyryl-CoA into crotonyl-CoA; butyryl-CoA dehydrogenase (Cbei_0322, Cbei_2035, Cbei_2883) catalyzing crotonyl-CoA into butyryl-CoA ( Figures 3D and 4C ; Supplementary Table S5f...”
HMPREF1322_RS01760 electron transfer flavoprotein subunit alpha/FixB family protein from Porphyromonas gingivalis W50
PG1078 electron transfer flavoprotein, alpha subunit from Porphyromonas gingivalis W83
42% identity, 80% coverage
CD0401 electron transfer flavoprotein alpha-subunit from Clostridium difficile 630
CD630_04010, CDIF630erm_00529 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridioides difficile
42% identity, 79% coverage
- Semiquantitative analysis of clinical heat stress in Clostridium difficile strain 630 using a GeLC/MS workflow with emPAI quantitation
Ternan, PloS one 2014 - “...proteins necessary for the reductive branch of the leucine fermentation pathway encoded by genes CD0394 CD0401 [38] with the sole exception of the ATP-dependent activator protein, hadI. We subsequently identified hadI (CD0396) in our iTRAQ investigation, where, like hadA (CD0395), hadB (CD0397) and hadC (CD0298), its...”
- Iron Regulation in Clostridioides difficile
Berges, Frontiers in microbiology 2018 - “...-4.27 -10.17 -4.76 CD630_04000 CDIF630erm_00528 etfB1 Electron transfer flavoprotein subunit beta -7.28 -3.90 -10.48 -4.50 CD630_04010 CDIF630erm_00529 etfA1 Electron transfer flavoprotein subunit alpha -7.50 -3.90 -10.39 -4.98 CD630_10540 CDIF630erm_01194 bcd2 Butyryl-CoA dehydrogenase -5.89 OFF -8.00 OFF CD630_10550 CDIF630erm_01195 etfB Electron transfer flavoprotein subunit beta -7.47 OFF...”
- “...-10.17 -4.76 CD630_04000 CDIF630erm_00528 etfB1 Electron transfer flavoprotein subunit beta -7.28 -3.90 -10.48 -4.50 CD630_04010 CDIF630erm_00529 etfA1 Electron transfer flavoprotein subunit alpha -7.50 -3.90 -10.39 -4.98 CD630_10540 CDIF630erm_01194 bcd2 Butyryl-CoA dehydrogenase -5.89 OFF -8.00 OFF CD630_10550 CDIF630erm_01195 etfB Electron transfer flavoprotein subunit beta -7.47 OFF -7.70...”
EZN00_RS10800 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium tyrobutyricum
42% identity, 77% coverage
MELS_2126, MELS_RS10960 electron transfer flavoprotein subunit alpha/FixB family protein from Megasphaera elsdenii DSM 20460
44% identity, 78% coverage
- The reductive half-reaction of two bifurcating electron-transferring flavoproteins: Evidence for changes in flavin reduction potentials mediated by specific conformational changes
Vigil, The Journal of biological chemistry 2022 - “...flavin adenine dinucleotide. Experimental procedures Organisms and growth conditions Plasmids containing the M.elsdenii EtfAB genes (MELS_2126 and MELS_2127) were constructed with a C-terminal Strep II tag derived from pASG-IBA3 (IBA GmbH) as previously described by Chowdhury etal. ( 5 ). The plasmids were transformed into chemically...”
- Spectral deconvolution of redox species in the crotonyl-CoA-dependent NADH:ferredoxin oxidoreductase from Megasphaera elsdenii. A flavin-dependent bifurcating enzyme
Vigil, Archives of biochemistry and biophysics 2021 - “...2. Experimental Organisms and Growth Conditions Construction of the plasmids containing M. elsdenii EtfAB ( Mels_2126 + Mels_2127 ) and bcd ( Mels_2128 ) genes and a C-terminus Strep II tag derived from pASG-IBA3 (IBA GmbH, Gttingen, Germany), were previously described by Chowdhury et al., 2015...”
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...(ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04240), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24820), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2710), C_acetobutylicum_ATTC824_GlcD (CA_C2544), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03160), C_butyricum_KNU-L09_GlcD (ATN24_RS03025), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08880), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24285), C_diolis_NJP7_GlcD (CCS79_RS18265), C_diolis_NJP7_LldP_GlcD (CCS79_RS09595),...”
WP_069875593 electron transfer flavoprotein subunit alpha/FixB family protein from Fusibacter sp. 3D3
41% identity, 76% coverage
ATN24_RS03165 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium butyricum
43% identity, 76% coverage
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...A_woodii_DSM1030_GlcD_LldP (AWO_RS04415), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07090), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04260), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04230), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24810), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2709), C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270),...”
- “...of the NZ_CP013252 chromosome of C. butyricum KNU-L09 containing genes encoding EtfA (1: ATN24_RS03030, 2: ATN24_RS03165, 3: ATN24_RS08885) and EtfB (1: ATN24_RS03025, 2: ATN24_RS03160, 3: ATN24_RS08880), and potentially GlcD (1: ATN24_RS03035, 3: ATN24_RS08895, 4: ATN24_RS11095) A similar search was performed for the selected genomes of bacteria...”
ELI_0542, KR505_10020 electron transfer flavoprotein subunit alpha/FixB family protein from Eubacterium callanderi
43% identity, 76% coverage
- Biosynthesis of butyrate from methanol and carbon monoxide by recombinant Acetobacterium woodii
Chowdhury, International microbiology : the official journal of the Spanish Society for Microbiology 2022 - “...KIST612 were selected and amplified by PCR. The genes clustered in the butyrate operon, ELI_0537 ELI_0542, consisting of thiolase ( thlA ), 3-hydroxybutyryl-CoA dehydrogenase (hbd ), crotonase ( crt ), butyryl-CoA dehydrogenase ( bcd ), and two subunits of electron transferring flavoprotein ( EtfA/B) were amplified...”
- “...region was also amplified by PCR and subcloned into the pMTL84211_6kb plasmid, downstream of etfA (ELI_0542). The resulting plasmid had 7 genes ( thl , hbd , crt , bcd/EtfAB , and ptb ) of the butyrate synthetic pathway and was called pMTL84211Ack_NP_But7. A control plasmid...”
- Gut Microbiota Eubacterium callanderi Exerts Anti-Colorectal Cancer Activity
Ryu, Microbiology spectrum 2022 - “...(locus_tag, KR505_02670); Bcd , butyryl-CoA dehydrogenase (KR505_02675, KR505_10030); ETF , electron transfer flavoprotein subunit alpha (KR505_10020); ETF , electron transfer flavoprotein subunit beta (KR505_10025); Hbd , hydroxybutyryl dehydrogenase (KR505_10035); Cro , crotonase/enoyl-CoA hydratase (KR505_10040); Thl , acetyl-CoA C-acetyltransferase (KR505_10045). (B) Series of genes related to GABA...”
- Energy Conservation Model Based on Genomic and Experimental Analyses of a Carbon Monoxide-Utilizing, Butyrate-Forming Acetogen, Eubacterium limosum KIST612
Jeong, Applied and environmental microbiology 2015 - “...bcd gene, there are two genes (etfAB; ELI_0541 and ELI_0542) encoding two subunits of a potential Etf protein. This gene cluster is well conserved in butyrate-...”
CBO3197 electron transfer flavoprotein alpha-subunit from Clostridium botulinum A str. ATCC 3502
41% identity, 79% coverage
- The cold-induced two-component system CBO0366/CBO0365 regulates metabolic pathways with novel roles in group I Clostridium botulinum ATCC 3502 cold tolerance
Dahlsten, Applied and environmental microbiology 2014 - “...short-chain specific acyl-CoA dehydrogenase and cbo3198 and cbo3197 encoding electron transfer flavoprotein alpha- and beta subunits (etfAB). An EMSA analysis...”
- Transcriptomic analysis of (group I) Clostridium botulinum ATCC 3502 cold shock response
Dahlsten, PloS one 2014 - “...cbo0558A , cbo0751, cbo0753, cbo1323 , cbo1407, cbo2226, cbo2227, cbo2304 , cbo2525, cbo2847, cbo2961 , cbo3197 , and cbo3202 ) on samples used for microarray hybridizations. The Cq values of the 1-h post-cold-shock samples for genes cbo0751, cbo0753, cbo1407, cbo2226, cbo2227, cbo2525, cbo2847, cbo3199 , and...”
- “...of 120 ( cbo0097 , cbo0477 , cbo0558A , cbo1323 , cbo2304 , cbo2961 , cbo3197 , cbo3202 ), 110 3 ( cbo0751, cbo0753, cbo1407, cbo2226, cbo2227, cbo2525, cbo2847 ), or 110 5 (16S rrn ) diluted cDNA in a total volume of 20 l. The...”
- Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clusters
Raphael, Applied and environmental microbiology 2008 - “...CBO0640, CBO0652, CBO0881, CBO1050, CBO2468, CBO2615, and CBO3197 also failed to produce amplification products using the appropriate primers. Analysis of the...”
ROSINTL182_RS00140 electron transfer flavoprotein subunit alpha/FixB family protein from Roseburia intestinalis L1-82
40% identity, 77% coverage
GSU2796 electron transfer flavoprotein, alpha subunit from Geobacter sulfurreducens PCA
41% identity, 73% coverage
B0M9M9 Electron transfer flavoprotein FAD-binding domain protein from Anaerostipes caccae (strain DSM 14662 / CCUG 47493 / JCM 13470 / NCIMB 13811 / L1-92)
40% identity, 76% coverage
Clocel_2973 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium cellulovorans 743B
41% identity, 77% coverage
- Clostridium cellulovorans Proteomic Responses to Butanol Stress
Costa, Frontiers in microbiology 2021 - “...products (i.e., acetyl-CoA acetyltransferases, Clocel_0192 and Clocel_3058; 3-hydroxybutyryl-CoA dehydrogenase, Clocel_2972; electron transfer flavoprotein and subunits, Clocel_2973 and Clocel_2974; and phosphate butyryltransferase, Clocel_3675) out of ten involved in acetyl-CoA conversion to butyrate were down-regulated ( Figure 8 ). In addition, slight down-regulation of enoyl-CoA hydratase/isomerase (Clocel_2976) and...”
- “...pathway (i.e., acetyl-CoA acetyltransferase, Clocel_0192 and Clocel_3058; 3-hydroxybutyryl-CoA dehydrogenase, Clocel_2972; electron transfer flavoprotein and subunits, Clocel_2973 and Clocel_2974; and phosphate butyryltransferase, Clocel_3675) ( Figure 8 ). Although the metabolic reason for this re-arrangement is currently unclear, one can hypothesize that down-regulation of the butyrate pathway may...”
5ol2A / A0A125V455 The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
43% identity, 76% coverage
- Ligands: flavin-adenine dinucleotide; calcium ion (5ol2A)
Q18AQ5 butanoyl-CoA dehydrogenase complex (NAD+, ferredoxin) (subunit 1/3) (EC 1.3.1.109) from Clostridioides difficile (see paper)
CD1056 electron transfer flavoprotein alpha-subunit from Clostridium difficile 630
CD630_10560, CDIF630erm_01196 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridioides difficile 630
43% identity, 76% coverage
- Diverse Energy-Conserving Pathways in Clostridium difficile: Growth in the Absence of Amino Acid Stickland Acceptors and the Role of the Wood-Ljungdahl Pathway
Gencic, Journal of bacteriology 2020 (secret) - Effect of an oxygen-tolerant bifurcating butyryl coenzyme A dehydrogenase/electron-transferring flavoprotein complex from Clostridium difficile on butyrate production in Escherichia coli
Aboulnaga, Journal of bacteriology 2013 - “...etfB2), electron transfer flavoprotein subunit alpha (CD1056, etfA2), crotonase (EC 4.2.1.17) (CD1057, crt2), 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157)...”
- Comparative transcriptional analysis of clinically relevant heat stress response in Clostridium difficile strain 630
Ternan, PloS one 2012 - “...acetyl-CoA and formate to CoA and pyruvate, during metabolism of propanoate and butanoate. CD1054 and CD1056, encoding butyryl-CoA dehydrogenase ( bcd2 ) and an electron transfer flavoprotein alpha-subunit ( etfA2 ), two enzymes of the butanoate metabolic pathway were also upregulated. The phosphotransferase (PTS) sugar transport...”
- The WalRK Two-Component System Is Essential for Proper Cell Envelope Biogenesis in Clostridioides difficile
Müh, Journal of bacteriology 2022 - “...Acyl-CoA dehydrogenase 1.93 2.23 1.03 cd630_10550 10550 etfB Electron transfer flavoprotein beta 1.78 2.11 1.07 cd630_10560 10560 etfA Electron transfer flavoprotein alpha 1.95 2.18 1.10 cd630_10570 10570 crt2 3-Hydroxybutyryl-CoA dehydratase 1.96 2.27 1.06 cd630_31000 31000 C4-dicarboxylate anaerobic carrier 1.88 2.59 NC Membrane cd630_30990 30990 Amidohydrolase 1.80...”
- Iron Regulation in Clostridioides difficile
Berges, Frontiers in microbiology 2018 - “...OFF -8.00 OFF CD630_10550 CDIF630erm_01195 etfB Electron transfer flavoprotein subunit beta -7.47 OFF -7.70 OFF CD630_10560 CDIF630erm_01196 etfA Electron transfer flavoprotein subunit alpha -6.75 OFF -7.75 OFF CD630_10570 CDIF630erm_01197 crt2 3-hydroxybutyryl-CoA dehydratase -6.37 -7.48 OFF CD630_10580 CDIF630erm_01198 hbd 3-hydroxybutyryl-CoA dehydrogenase -5.21 OFF -5.97 OFF CD630_10590 CDIF630erm_01199...”
- “...-8.00 OFF CD630_10550 CDIF630erm_01195 etfB Electron transfer flavoprotein subunit beta -7.47 OFF -7.70 OFF CD630_10560 CDIF630erm_01196 etfA Electron transfer flavoprotein subunit alpha -6.75 OFF -7.75 OFF CD630_10570 CDIF630erm_01197 crt2 3-hydroxybutyryl-CoA dehydratase -6.37 -7.48 OFF CD630_10580 CDIF630erm_01198 hbd 3-hydroxybutyryl-CoA dehydrogenase -5.21 OFF -5.97 OFF CD630_10590 CDIF630erm_01199 thlA1...”
HMPREF0389_00742 electron transfer flavoprotein subunit alpha/FixB family protein from Filifactor alocis ATCC 35896
42% identity, 76% coverage
- Proteome variation among Filifactor alocis strains
Aruni, Proteomics 2012 - “...fraction. 262 kDa: cell wall associated serine proteinase (HMPREF0389_01110); 35 kDa: electron transfer flavo-protein alpha (HMPREF0389_00742); 14 kDa: neutrophil-activating protein A (HMPREF0389_01654). Figure 3 2D-PAGE of the membrane fraction of F. alocis ATCC 35896 strain. 2D page was performed using 7-cm IPG strips of p I...”
- “...barrel protein 36.2/36.15 38/0.09 5 3.564 C 0.117 M Phosphate-binding domain 0.56 Nonsecretory protein 34. HMPREF0389_00742 Electron transfer flavoprotein alpha subunit protein 35/35.1 246/0.43 15 0.160 M ETF electron acceptor domain 0.56 Nonsecretory protein 35. HMPREF0389_01141 Hypothetical protein 30/30 24/0.11 9 4.65 C 0.362 M No...”
TTE0547 Electron transfer flavoprotein alpha-subunit from Thermoanaerobacter tengcongensis MB4
44% identity, 78% coverage
SZ51_09315 electron transfer flavoprotein subunit alpha/FixB family protein from Brachyspira hyodysenteriae
43% identity, 79% coverage
- The Exposed Proteomes of Brachyspira hyodysenteriae and B. pilosicoli
Casas, Frontiers in microbiology 2016 - “...Rod shape-determining protein MreB 162 A0A0H0TUE9 SR30_05720 BRAHW Electron transfer flavoprotein subunit alpha 98 A0A0H0VH59 SZ51_09315 BRAHW Ferric uptake regulator 59 D8IDK7 fur BRAPL Ferredoxin 4Fe 4S 40 D8IA49 BP951000_2206 BRAPL Phage terminase large subunit 26 D8IE54 xtmB BRAPL Pyruvate ferredoxin/flavodoxin oxidoreductase 23 D8IED5 porA X...”
TTHE_RS08240 electron transfer flavoprotein subunit alpha/FixB family protein from Thermoanaerobacterium thermosaccharolyticum DSM 571
41% identity, 77% coverage
CG447_RS01775 acryloyl-CoA reductase electron transfer subunit beta from Faecalibacterium duncaniae
41% identity, 78% coverage
EHLA_0976 electron transfer flavoprotein subunit alpha/FixB family protein from Anaerobutyricum hallii
40% identity, 76% coverage
- Unravelling lactate-acetate and sugar conversion into butyrate by intestinal Anaerobutyricum and Anaerostipes species by comparative proteogenomics
Shetty, Environmental microbiology 2020 - “...shared between the two comparisons (including those encoded by genes with locus tags EHLA_0973, EHLA_0974, EHLA_0976, EHLA_0977, EHLA_0978, EHLA_0979) that were induced by growth on d , l lactate. Proteins with significantly higher abundance during growth on d , l lactate included lactate permease, lactate dehydrogenase,...”
bhn_I2221 electron transfer flavoprotein subunit alpha/FixB family protein from Butyrivibrio hungatei
38% identity, 77% coverage
- Butyrivibrio hungatei MB2003 Competes Effectively for Soluble Sugars Released by Butyrivibrio proteoclasticus B316T during Growth on Xylan or Pectin
Palevich, Applied and environmental microbiology 2019 - “...GH25, 2 SH3 1.73E18 2.17 MB2003 coculture growth bhn_I0681 agn53A Arabinogalactan endo-1,4--galactosidase GH53 1.78E+10 3.7 bhn_I2221 gh31C Glycoside hydrolase family 31 GH31 1.73E+40 2.98 bhn_I1841 bga35B -Galactosidase GH35 9.70E+11 2.66 bhn_I1532 arf51C - l -Arabinofuranosidase GH51/CBM4 4.33E+22 2.25 bhn_I1407 est4D Polysaccharide deacetylase CE4 5.31E03 2.23 bhn_I0167...”
- “...2.41 bhn_I0194 lyc25C Lysozyme GH25 2.32E02 2.13 bhn_I1969 Glycoside hydrolase GH130 family GH130/GH43 2.15E02 2.11 bhn_I2221 gh31C Glycoside hydrolase family 31 GH31 6.89E03 2.07 bhn_III72 est4A Polysaccharide deacetylase CE4 0.00E+00 N/A MB2003 coculture growth bhn_I1532 arf51C - l -Arabinofuranosidase GH51/CBM4 0.00E+00 5.05 bhn_I0847 cel9B Cellodextrinase GH9/CelD...”
- The complete genome sequence of the rumen bacterium Butyrivibrio hungatei MB2003
Palevich, Standards in genomic sciences 2017 - “...conversion to acetyl-CoA, as well as a butyryl-CoA dehydrogenase/electron transferring flavoprotein bcd - etfAB (bhn_I2225, bhn_I2221 and bhn_I2222) to generate ATP by classic substrate level phosphorylation. In addition, MB2003 possesses genes that encode all six subunits of the Rnf ( rnfA , rnfB , rnfC ,...”
BPHY_RS38175, Bphy_7738 electron transfer flavoprotein subunit alpha/FixB family protein from Paraburkholderia phymatum STM815
Bphy_7738 electron transfer flavoprotein alpha/beta-subunit from Burkholderia phymatum STM815
39% identity, 76% coverage
- Metabolomics and Dual RNA-Sequencing on Root Nodules Revealed New Cellular Functions Controlled by Paraburkholderia phymatum NifA
Bellés-Sancho, Metabolites 2021 - “...homocitrate synthase Bphy_7741 nifV 8.28 BPHY_RS37725 D-alanine-D-alanine ligase Bphy_7633 8.45 BPHY_RS38165 ferredoxin Bphy_7736 fixX 8.93 BPHY_RS38175 electron transfer flavoprotein subunit alpha/FixB family protein Bphy_7738 fixB 9.16 BPHY_RS36680 ABC transporter ATP-binding protein Bphy_7408 9.33 BPHY_RS38195 nitrogenase cofactor biosynthesis protein Bphy_7742 nifB 9.83 BPHY_RS38260 nitrogenase molybdenum-iron protein alpha...”
- “...Bphy_7633 8.45 BPHY_RS38165 ferredoxin Bphy_7736 fixX 8.93 BPHY_RS38175 electron transfer flavoprotein subunit alpha/FixB family protein Bphy_7738 fixB 9.16 BPHY_RS36680 ABC transporter ATP-binding protein Bphy_7408 9.33 BPHY_RS38195 nitrogenase cofactor biosynthesis protein Bphy_7742 nifB 9.83 BPHY_RS38260 nitrogenase molybdenum-iron protein alpha chain Bphy_7754 nifD 10.05 BPHY_RS36030 twin-arginine translocation signal...”
- Metabolomics and Transcriptomics Identify Multiple Downstream Targets of Paraburkholderia phymatum σ54 During Symbiosis with Phaseolus vulgaris
Lardi, International journal of molecular sciences 2018 - “...nitrogenase molybdenum-cofactor biosynthesis protein nifN 2.9 Bphy_7733 ferredoxin III, nif-specific 5.0 Bphy_7737 electron-transferring-flavoprotein dehydrogenase 4.2 Bphy_7738 electron transfer flavoprotein /-subunit 4.5 Bphy_7739 electron transfer flavoprotein /-subunit 5.2 Bphy_7754 nitrogenase molybdenum-iron protein chain nifD 4.3 Bphy_7755 nitrogenase molybdenum-iron protein chain nifK 4.0 Bphy_7803 electron transfer flavoprotein subunit...”
- Transcriptome Analysis of Paraburkholderia phymatum under Nitrogen Starvation and during Symbiosis with Phaseolus Vulgaris
Lardi, Genes 2017 - “...biosynthesis protein nifN 3.7 Bphy_7733 ferredoxin III, nif -specific 6.3 Bphy_7737 electron-transferring-flavoprotein dehydrogenase fixC 6.8 Bphy_7738 electron transfer flavoprotein /-subunit fixB 7.1 Bphy_7739 electron transfer flavoprotein /-subunit fixA 7.8 Bphy_7754 nitrogenase molybdenum-iron protein chain nifD 8.4 Bphy_7755 nitrogenase molybdenum-iron protein chain nifK 8.3 Bphy_7804 electron transfer...”
SMa0819 electron transfer flavoprotein subunit alpha/FixB family protein from Sinorhizobium meliloti 1021
38% identity, 76% coverage
- Co-catabolism of arginine and succinate drives symbiotic nitrogen fixation
Flores-Tinoco, Molecular systems biology 2020 - “...components of the nitrogenase nifHDK (SMa0825, SMa0827, SMa0829), the electron bifurcation complex fixABCX (SMa0816, SMa0817, SMa0819, SMa0822), and the alternative complex IV fixNOQP 13 among the topranked symbiosis genes in the TnSeq dataset ( Dataset EV3 ). These genetic evidences support a model in which the...”
FN0785 Electron transfer flavoprotein alpha-subunit from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
46% identity, 67% coverage
CLNEO_21760 acryloyl-CoA reductase electron transfer subunit beta from Anaerotignum neopropionicum
39% identity, 80% coverage
acrA / G3KIM6 electron-transferring flavoprotein α subunit from Anaerotignum propionicum (see paper)
ETFA_ANAPI / G3KIM6 Acryloyl-CoA reductase electron transfer subunit beta; Electron transfer flavoprotein large subunit; ETFLS; Electron transfer flavoprotein subunit beta; Beta-ETF from Anaerotignum propionicum (Clostridium propionicum) (see paper)
G3KIM6 acrylyl-CoA reductase (NADH) (EC 1.3.1.95) from Anaerotignum propionicum (see paper)
40% identity, 79% coverage
- function: Part of the ETF-acryloyl-CoA reductase complex involved in the pathway of L-alanine fermentation. The electron transfer flavoprotein (ETF) serves as a specific electron acceptor for acryloyl- CoA reductase.
subunit: Heterohexadecamer; tetramer of tetramers. Each tetramer is composed of 2 alpha (AcrC), a beta (AcrA) and a gamma (AcrB) subunit.
YP_001796224 Electron transfer flavoprotein, alpha subunit from Cupriavidus taiwanensis
36% identity, 85% coverage
- Plant-bacteria association and symbiosis: are there common genomic traits in alphaproteobacteria?
Pini, Genes 2011 - “...Electron transfer flavoprotein, beta subunit YP_001796225 2898 Unknown YP_002236173 2908 Electron transfer flavoprotein, alpha subunit YP_001796224 2912 Methyltransferase YP_935409 YP_001176000 YP_002237443 YP_001177854 YP_002239590 2927 Aminoacid aldolase or racemase YP_002007445 2981 Mg 2+ and Co 2+ transporters YP_002006900 YP_001176870 YP_002238775 YP_002238859 3082 Ferredoxin-like protein YP_001796222 3137 Unknown...”
NGR_a01230 nitrogen fixation protein FixB; electron transfer flavoprotein alpha chain from Rhizobium sp. NGR234
NGR_a01230 electron transfer flavoprotein subunit alpha/FixB family protein from Sinorhizobium fredii NGR234
39% identity, 76% coverage
Q6N105 Electron transfer flavoprotein alpha chain protein fixB from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
38% identity, 76% coverage
Avin_10530 electron transfer flavoprotein, alpha subunit, FixB from Azotobacter vinelandii AvOP
38% identity, 78% coverage
- The Electron Bifurcating FixABCX Protein Complex from Azotobacter vinelandii: Generation of Low-Potential Reducing Equivalents for Nitrogenase Catalysis
Ledbetter, Biochemistry 2017 - “...fix operon, which includes six genes ( fixfd , Avin_10510; fixA , Avin_10520; fixB , Avin_10530; fixC , Avin_10540; fixX , Avin_10550; ORF6, Avin_10560) ( Figure S2 ), was PCR amplified using primers specified in Table S2 . The PCR product was digested with Xba I...”
- Defining Electron Bifurcation in the Electron-Transferring Flavoprotein Family
Garcia, Journal of bacteriology 2017 - “...FixB (Etf-) from Azotobacter vinelandii (STRING network identifier: Avin_10530) serving as the query. A total of 1,314 Etf- homologs across 890 genomes were...”
AZC_3448 FixB protein from Azorhizobium caulinodans ORS 571
37% identity, 76% coverage
- Comparative genome-wide transcriptional profiling of Azorhizobium caulinodans ORS571 grown under free-living and symbiotic conditions
Tsukada, Applied and environmental microbiology 2009 - “...genes (AZC_1038, AZC_1040, AZC_1049, AZC_3414, AZC_3446, AZC_3448, and AZC_3449), a putative transcriptional factor gene (phrR; AZC_0013), a...”
- The genome of the versatile nitrogen fixer Azorhizobium caulinodans ORS571
Lee, BMC genomics 2008 - “...Nitrogenase MoFe cofactor biosynthesis AZC_3446 nif W Nitrogenase-stabilizing/protective protein AZC_3447 fix A Electron-transferring flavoprotein oxidoreductase AZC_3448 fix B Electron-transferring flavoprotein AZC_3449 fix C Electron-transferring flavoprotein oxidoreductase AZC_3450 fix X Ferredoxin protein AZC_3925 rpo N RNA polymerase 54 factor AZC_4523 cyt N /fix N Cytochrome c oxidase...”
D2RIQ3 butanoyl-CoA dehydrogenase complex (NAD+, ferredoxin) (subunit 2/3) (EC 1.3.1.109); short-chain acyl-CoA dehydrogenase (EC 1.3.8.1) from Acidaminococcus fermentans (see paper)
Acfer_0556 Electron transfer flavoprotein alpha subunit from Acidaminococcus fermentans DSM 20731
38% identity, 77% coverage
4kpuA / D2RIQ3 Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
38% identity, 77% coverage
- Ligand: flavin-adenine dinucleotide (4kpuA)
A6A40_02190 electron transfer flavoprotein subunit alpha/FixB family protein from Azospirillum humicireducens
38% identity, 76% coverage
- Complete genome sequence of the nitrogen-fixing bacterium Azospirillum humicireducens type strain SgZ-5T
Yu, Standards in genomic sciences 2018 - “...fixation Locus Tag Size/aa Gene Gene product A6A40_02185 852 fixA Electron transfer flavoprotein beta subunit A6A40_02190 1080 fixB Electron transfer flavoprotein alpha chain A6A40_02195 1302 fixC Flavoprotein-ubiquinone oxidoreductase A6A40_09085 210 fixU Nitrogen fixation protein A6A40_02200 285 fixX Ferredoxin-like protein A6A40_09040 1866 nifA Nif-specific transcriptional activator A6A40_09050...”
- “...in the nitrogen fixation gene cluster of 176.7kb. Besides, an operon containing fixABCX genes (A6A40_02185, A6A40_02190, A6A40_02195 and A6A40_02220) responsible for electron transfer to nitrogenase is located upstream of this gene cluster. Nevertheless, the fixABCX operon is generally regulated by a transcriptional activator NifA protein for...”
PGN_0800 putative electron transfer flavoprotein alpha subunit from Porphyromonas gingivalis ATCC 33277
39% identity, 75% coverage
- Synthesis of ppGpp impacts type IX secretion and biofilm matrix formation in Porphyromonas gingivalis
Kim, NPJ biofilms and microbiomes 2020 - “...d -erythritol 2,4-cyclodiphosphate synthase 2.17 PGN_0310 Uroporphyrinogen-III synthase 1.90 PGN_0532 Magnesium chelatase subunit I 1.88 PGN_0800 Electron transfer flavoprotein alpha subunit 1.75 PGN_1239 Lipopolysaccharide biosynthesis glycosyltransferase 2.29 PGN_1370 NAD-dependent nucleotidediphosphatesugar epimerase 2.23 PGN_1375 -ketoacylacyl carrier protein reductase 1.94 PGN_1705 acpP Acyl carrier protein 2.98 Transfer RNA...”
PG0776 electron transfer flavoprotein, alpha subunit from Porphyromonas gingivalis W83
38% identity, 75% coverage
- Galactose Impacts the Size and Intracellular Composition of the Asaccharolytic Oral Pathobiont Porphyromonas gingivalis
Moye, Applied and environmental microbiology 2019 - “...PG0618 PG0619 PG0648 PG0681 PG0684 PG0717 PG0718 PG0775 PG0776 PG0777 PG0778 PG0835 PG0901 PG0987 PG1020 PG1110 PG1148 PG1167 PG1205 PG1240 PG1306 PG1357 PG1374...”
- Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
Moon, BMC microbiology 2014 - “...PG0897 Alpha-amylase family protein 1.85 PG1793 1,4-alpha-glucan branching enzyme 1.67 Energy metabolism : Electron transport PG0776 Electron transfer flavoprotein, alpha subunit 2.30 PG0777 Electron transfer flavoprotein, beta subunit 1.91 PG1638 Thioredoxin family protein 1.88 PG1332 NAD(P) transhydrogenase, beta subunit 1.83 PG1119 Flavodoxin, putative 1.69 PG0429 Pyruvate...”
- HcpR of Porphyromonas gingivalis is required for growth under nitrosative stress and survival within host cells
Lewis, Infection and immunity 2012 - “...coding for thioredoxin, PG1227, encoding LysR-type regulator, PG0776 and PG0900, coding for oxidoreductases, PG1318, encoding ECF sigma protein, PG1129,...”
- “...Mf Fldb Pc Repeatd PG0893 PG0616 PG1237 PG1185 PG0776 PG1335 PG1860 PG2027 PG1318 PG0605 PG0900 PG0752 PG1129 PG1321 PG0456 PG0931 PG2167 PG2132 PG0313 PG1171...”
- Nitric oxide stress resistance in Porphyromonas gingivalis is mediated by a putative hydroxylamine reductase
Boutrin, Journal of bacteriology 2012 - “...Additionally, 12 upregulated genes (PG1858, dps, PG0777, PG0776, PG1172, PG1171, PG1239, hcp, PG2034, PG0108, PG0616, and PG0195) that were differentially...”
- Effects of various growth conditions in a chemostat on expression of virulence factors in Porphyromonas gingivalis
Masuda, Applied and environmental microbiology 2006 - “...PG2024 PG0449 PG0506 PG2024 PG1824 PG1804 PG1385 PG0027 PG0343 PG0776 PG1755 PG1077 PG0378 PG1545 PG0293 PG0618 Fold changec 0.15 0.09 1.75 0.90 1.02 0.69 0.77...”
RLV_1891 electron transfer flavoprotein subunit alpha/FixB family protein from Rhizobium leguminosarum bv. viciae
38% identity, 76% coverage
HFX_1926 electron transfer flavoprotein subunit alpha/FixB family protein from Haloferax mediterranei ATCC 33500
36% identity, 78% coverage
- Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei
Payá, International journal of molecular sciences 2024 - “...oxidase subunit II 0.89 2.82 HFX_1927 electron transfer flavoprotein subunit beta/FixA family protein 0.49 3.14 HFX_1926 electron transfer flavoprotein subunit alpha/FixB family protein 0.53 3.33 Genes that encode stress proteins HFX_0946 universal stress protein 0.22 2.05 HFX_1094 universal stress protein 0.94 2.09 HFX_2288 universal stress protein...”
pRL100199 FixB electron transfer protein from Rhizobium leguminosarum bv. viciae 3841
37% identity, 76% coverage
BSU28520 electron transfer flavoprotein (alpha subunit) from Bacillus subtilis subsp. subtilis str. 168
NP_390730 electron transfer flavoprotein (alpha subunit) from Bacillus subtilis subsp. subtilis str. 168
P94551 Electron transfer flavoprotein subunit alpha from Bacillus subtilis (strain 168)
40% identity, 74% coverage
BBta_5871 protein FixB from Bradyrhizobium sp. BTAi1
36% identity, 76% coverage
MELS_RS10255 electron transfer flavoprotein subunit alpha/FixB family protein from Megasphaera elsdenii DSM 20460
36% identity, 78% coverage
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04240), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24820), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2710), C_acetobutylicum_ATTC824_GlcD (CA_C2544), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03160), C_butyricum_KNU-L09_GlcD (ATN24_RS03025), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08880), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24285),...”
PAE0721 electron transfer flavoprotein alpha subunit (etfA) from Pyrobaculum aerophilum str. IM2
38% identity, 80% coverage
Dred_1779 electron transfer flavoprotein, alpha subunit from Desulfotomaculum reducens MI-1
37% identity, 76% coverage
DSY1714 hypothetical protein from Desulfitobacterium hafniense Y51
36% identity, 76% coverage
Bdiaspc4_08985 electron transfer flavoprotein subunit alpha/FixB family protein from Bradyrhizobium diazoefficiens
blr1773 electron transfer flavoprotein alpha chain from Bradyrhizobium japonicum USDA 110
36% identity, 76% coverage
GJQ69_08635 electron transfer flavoprotein subunit alpha/FixB family protein from Caproicibacterium lactatifermentans
40% identity, 73% coverage
- Revealing the Characteristics of Glucose- and Lactate-Based Chain Elongation for Caproate Production by Caproicibacterium lactatifermentans through Transcriptomic, Bioenergetic, and Regulatory Analyses
Wang, mSystems 2022 - “...required for FAD-dependent enzymes, e.g., lactate dehydrogenase (GJQ69_08645), butyryl-CoA dehydrogenase (GJQ69_08630), and electron transfer flavoproteins (GJQ69_08635 and GJQ69_08640). As indicated in Fig.3 , these FAD-dependent enzymes involved in lactate utilization and caproate production were all significantly upregulated under the lactate condition. Therefore, the active synthesis of...”
- Adaptability of a Caproate-Producing Bacterium Contributes to Its Dominance in an Anaerobic Fermentation System
Wang, Applied and environmental microbiology 2021 (secret)
CCS79_RS18965 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium diolis
45% identity, 58% coverage
TM1531 electron transfer flavoprotein, alpha subunit from Thermotoga maritima MSB8
WP_004081904 electron transfer flavoprotein subunit alpha/FixB family protein from Thermotoga sp. Mc24
40% identity, 75% coverage
- Characterization of the Membrane-Associated Electron-Bifurcating Flavoenzyme EtfABCX from the Hyperthermophilic Bacterium Thermotoga maritima
Ge, Biochemistry 2023 - “...XLSX ) Supplementary Material bi3c00473_si_001.pdf bi3c00473_si_002.xlsx Accession Codes T. maritima Fix/EtfABCX, EtfA Tm1530 WP_004081902, EtfB Tm1531 WP_004081904, EtfC Tm1532 WP_004081907, EtfX Tm1533 WP_004081910. Author Contributions X.G., G.S., and M.A. designed this study, performed the experiments, analyzed, and interpreted the data and M.A. directed the research. J.T.,...”
- Parsing redox potentials of five ferredoxins found within Thermotoga maritima
Maiocco, Protein science : a publication of the Protein Society 2019 - “...the genomic region surrounding TM1533 shows that TM1530, TM1531, and TM1532 are FixA, FixB, and FixC, respectively, completing the FixABCX nitrogen fixation...”
- Transcriptional analysis of biofilm formation processes in the anaerobic, hyperthermophilic bacterium Thermotoga maritima
Pysz, Applied and environmental microbiology 2004 - “...7.1 8.6 6.3 6.0 6.0 TM1529 TM1530 TM1531 TM1532 TM1533 TM1534 TM1535 TM1536 Endoglucanase (intracellular) Endoglucanase (extracellular) Hypothetical protein...”
- Characterization of the Membrane-Associated Electron-Bifurcating Flavoenzyme EtfABCX from the Hyperthermophilic Bacterium Thermotoga maritima
Ge, Biochemistry 2023 - “...) Supplementary Material bi3c00473_si_001.pdf bi3c00473_si_002.xlsx Accession Codes T. maritima Fix/EtfABCX, EtfA Tm1530 WP_004081902, EtfB Tm1531 WP_004081904, EtfC Tm1532 WP_004081907, EtfX Tm1533 WP_004081910. Author Contributions X.G., G.S., and M.A. designed this study, performed the experiments, analyzed, and interpreted the data and M.A. directed the research. J.T., D.N.,...”
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
40% identity, 75% coverage
- Ligand: flavin-adenine dinucleotide (7koeB)
BF9343_3117 electron transfer flavoprotein subunit alpha/FixB family protein from Bacteroides fragilis NCTC 9343
37% identity, 75% coverage
SSO2817 Electron transfer flavoprotein alpha and beta-subunit (etfAB/fixAB) from Sulfolobus solfataricus P2
35% identity, 53% coverage
- Global analysis of viral infection in an archaeal model system
Maaty, Frontiers in microbiology 2012 - “...Succinyl-CoA synthetase beta subunit, SSO2483 (12, 24, 48, 72); Rubrerythrin, SSO2642 (12,24,36); Electron transfer flavoprotein, SSO2817 (48) Amino acid transport and metabolism X-pro aminopeptidase, SSO0010 (12,24); Prolidase, SSO0363 (48,72); d -3-phosphoglycerate dehydrogenase, SO0905 (36); FkbR2, putative, SSO1154 (12,24,36,48); O -succinylhomoserine (thiol)-lyase, SSO2368 (72); 3-isopropylmalate dehydratase, SSO2471...”
- Dynamic metabolic adjustments and genome plasticity are implicated in the heat shock response of the extremely thermoacidophilic archaeon Sulfolobus solfataricus
Tachdjian, Journal of bacteriology 2006 - “...nuoN SSO0584 SSO2348 SSO2353 SSO2762 SSO2763 SSO2776 SSO2817 SSO2819 SSO0044 doxB SSO0045 doxC SSO5098 doxE SSO0368 trxA-1 SSO2232 trxA-2 SSO2416 SSO2765...”
SYN_RS12515 electron transfer flavoprotein subunit alpha/FixB family protein from Syntrophus aciditrophicus SB
37% identity, 76% coverage
FRAAL5877 Electron transfer flavoprotein alpha-subunit (Alpha-ETF) (Electron transfer flavoprotein large subunit) (ETFLS) from Frankia alni ACN14a
37% identity, 77% coverage
CCI6_RS13315 electron transfer flavoprotein subunit alpha/FixB family protein from Frankia sp. CcI6
36% identity, 77% coverage
- Genomic, transcriptomic, and proteomic approaches towards understanding the molecular mechanisms of salt tolerance in Frankia strains isolated from Casuarina trees
Oshone, BMC genomics 2017 - “...Protein Name MW (Da) PI NaCl Sucrose [C] Energy production and Conversion CcI6 10 563,313,506 CCI6_RS13315 electron transfer flavoprotein alpha subunit apoprotein 32,842.70 5.01 15 563,312,797 CCI6_RS16290 NAD-dependent aldehyde dehydrogenase 54,456.60 5.40 N/C 1 563,313,455 CCI6_RS13080 ATP synthase F1 subcomplex beta subunit 56,735.20 4.56 N/C 8...”
- “...from the same spot (spot no. 10) was an electron transfer flavoprotein alpha subunit apoprotein (CCI6_RS13315), which, as expected, had a lower peptide count in the NaCl-treated samples. Several pyridoxal phosphate-binding proteins, including cysteine synthase, are differentially expressed under salt stress in wheat chloroplasts and help...”
NCU08004 electron transfer flavoprotein alpha-subunit from Neurospora crassa OR74A
37% identity, 74% coverage
Sfum_0107 electron transfer flavoprotein, alpha subunit from Syntrophobacter fumaroxidans MPOB
34% identity, 73% coverage
MAV_3875 electron transfer flavoprotein, alpha subunit from Mycobacterium avium 104
34% identity, 76% coverage
AF0287 electron transfer flavoprotein, subunit alpha (etfA) from Archaeoglobus fulgidus DSM 4304
36% identity, 73% coverage
MAP3060c FixB from Mycobacterium avium subsp. paratuberculosis str. k10
Q73VF4 FixB from Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10)
34% identity, 77% coverage
Mb3054c PROBABLE ELECTRON TRANSFER FLAVOPROTEIN (ALPHA-SUBUNIT) FIXB (ALPHA-ETF) (ELECTRON TRANSFER FLAVOPROTEIN LARGE SUBUNIT) (ETFLS) from Mycobacterium bovis AF2122/97
34% identity, 77% coverage
P9WNG9 Electron transfer flavoprotein subunit alpha from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Rv3028c PROBABLE ELECTRON TRANSFER FLAVOPROTEIN (ALPHA-SUBUNIT) FIXB (ALPHA-ETF) (ELECTRON TRANSFER FLAVOPROTEIN LARGE SUBUNIT) (ETFLS) from Mycobacterium tuberculosis H37Rv
34% identity, 77% coverage
- Using structural knowledge in the protein data bank to inform the search for potential host-microbe protein interactions in sequence space: application to Mycobacterium tuberculosis
Mahajan, BMC bioinformatics 2017 - “...P49411 tuf/Rv0685/MTCY210.02 TUFM P9WPU7 P25705 atpA/Rv1308/MTCY373.28 ATP5A1/ATP5A/ATP5AL2/ATPM P9WPU7 P06576 atpA/Rv1308/MTCY373.28 ATP5B/ATPMB/ATPSB P9WPU7 P36542 atpA/Rv1308/MTCY373.28 ATP5C1/ATP5C/ATP5CL1 P9WNG9 Q5T4U5 etfA/fixB/Rv3028c/MTV012.43c ACADM/hCG_22915 P9WNG9 P38117 etfA/fixB/Rv3028c/MTV012.43c ETFB/FP585 P9WNG9 B7Z9I1 etfA/fixB/Rv3028c/MTV012.43c ACADM P9WNG9 P11310 etfA/fixB/Rv3028c/MTV012.43c ACADM P9WMJ9 P38646 dnaK/Rv0350/MTCY13E10.10 HSPA9/GRP75/HSPA9B/mt-HSP70 Fig. 7 Functional annotation of top-ranking human proteins. The host proteins...”
- Integrated virtual screening and MD simulation study to discover potential inhibitors of mycobacterial electron transfer flavoprotein oxidoreductase
Arshad, PloS one 2024 - “...ACADs to menaquinone, comprising an electron transfer protein with two subunits, FixA (Rv3029c) and FixB (Rv3028c), along with a membrane-bound electron transfer flavoprotein-oxidoreductase, EtfD (Rv0338c) [ 11 ]. Deletion of the gene Rv0338c, encoding EtfD, disrupts -oxidation at the step catalyzed by ACADs, rendering mutants deficient...”
- Benzene Amide Ether Scaffold is Active against Non-replicating and Intracellular Mycobacterium tuberculosis
Ahmed, ACS infectious diseases 2023 - “...a gene ID gene name annotation Rv3043c ctaD cytochrome c oxidase polypeptideI (cytochrome AA3 subunit1) Rv3028c fixB electron transfer flavoprotein (alpha-subunit) Rv3582c ispD MEP cytidylyltransferase Rv2195 qcrA Ubiquinol-cytochrome c reductase ironsulfur subunit Rv2196 qcrB Ubiquinol-cytochrome c reductase (cytochromeB subunit) Rv1409 rpe bifunctional riboflavin biosynthesis protein Rv1462...”
- Multiple acyl-CoA dehydrogenase deficiency kills Mycobacterium tuberculosis in vitro and during infection
Beites, Nature communications 2021 - “...Mtb (annotated as fixA rv3029c ) forms an operon with etfA Mtb (annotated as fixB rv3028c ), which shares homology with the alpha subunit of the human ETF (41% identity: 98% coverage). In humans, ETF 24 interacts with a cognate membrane oxidoreductase 25 (EtfD) and both...”
- “...to rename the respective Mtb genes as etfD Mtb ( rv0338c ), etfB Mtb ( rv3028c ), and etfA Mtb ( rv3029c ). The increased susceptibility to fatty acids of an EtfD Mtb TetOff strain suggested a possible connection with fatty acid metabolism. Accordingly, our data...”
- One-Year Old Dormant, "Non-culturable" Mycobacterium tuberculosis Preserves Significantly Diverse Protein Profile
Trutneva, Frontiers in cellular and infection microbiology 2020 - “...Rv1310 Probable ATP synthase beta chain AtpD Rv2280 Probable dehydrogenase Rv2145c Diviva family protein Wag31 Rv3028c Probable electron transfer flavoprotein (alpha-subunit) FixB (alpha-ETF) (electron transfer flavoprotein large subunit) (ETFLS) Rv1886c Secreted antigen 85-B FbpB (85B) (antigen 85 complex B) (mycolyl transferase 85B) (fibronectin-binding protein B) (extracellular...”
- Comprehensive analysis of iron utilization by Mycobacterium tuberculosis
Zhang, PLoS pathogens 2020 - “...genes with such a pattern: rv0338c , rv0489 , rv0542c , rv0548c , rv1122 , rv3028c and rv3029c . However, none of these genes encoded transport proteins. In particular we did not see such a phenotype for the efflux pump MmpL11 which was shown to be...”
- Proteomics for the Investigation of Mycobacteria
Bespyatykh, Acta naturae 2017 - “...comparison of the resistant and susceptible strains revealed five proteins (Rv0491, Rv1446c, Rv2145, Rv2971, and Rv3028c) with increased representation in isoniazid-resistant strains [ 72 ]. These are membrane proteins that can potentially serve as targets for new therapeutic agents. An analysis of the aminoglycoside-resistant strains revealed...”
- A genome-wide structure-based survey of nucleotide binding proteins in M. tuberculosis
Bhagavat, Scientific reports 2017 - “...Probable aldehyde dehydrogenase AldC AMP ADP ATP GTP Rv2996c Probable D-3-phosphoglycerate dehydrogenase SerA1 (PGDH) ADP Rv3028c Probable electron transfer flavoprotein (alpha-subunit) FixB (alpha-ETF) (electron transfer flavoprotein large subunit) (ETFLS) GTP Rv3075c Conserved protein ATP GTP Rv3273 Probable transmembrane carbonic anhydrase (carbonate dehydratase) (carbonic dehydratase) ADP ATP...”
- “...proteins identified by our method (Rv0119, Rv0440, Rv0500A, Rv1391, Rv1843c, Rv1908c, Rv2145c, Rv2215, Rv2461c, Rv2783c, Rv3028c, Rv3336c and Rv3389c), to be validated by all three proteomics methods. Two other databases namely the Patric database 42 and TBDB 43 are widely used and serve as excellent resources...”
- Proteomics Analysis of Three Different Strains of Mycobacterium tuberculosis under In vitro Hypoxia and Evaluation of Hypoxia Associated Antigen's Specific Memory T Cells in Healthy Household Contacts
Devasundaram, Frontiers in microbiology 2016 - “...6.2 20% MMLGPNAADWPLILADASQPLTLEAMAAR, STAVLLAQSGLALALDRDR RvD5 Rv2145c Wag31 35 4.5 45% INELDQELAAGGGAGVTPQATQAIPAYEPEPGK, TNTAKAESDK, TRLKTYLESQLEELGQ, GSAAPVDSNADAGGFDQFNRGK RvD6 Rv3028c Probable electron transfer flavo protein 32 4.5 8% TVSPQLYIALGISGAIQHR RvD7 Rv1886c Ag85B mycolyl transferase 30 5.3 24% VQFQSGGNNSPAVYLLDGLR, NDPTQQIPK RvD8 Rv2780 Secreted L -alanine dehydrogenase ALD 32 6.1 21% GHEVLIQAGAGEGSAITDADFK,...”
- “...hypoxia in H37Rv and characterized as Rv0440 (GroEL2), Rv1240 [probable malate dehydrogenase (MDH)], Rv2145c (Wag31), Rv3028c (Flavoprotein), Rv1886c (Ag85B), Rv2780 (alanine dehydrogenase), Rv0854 (conserved protein), Rv2445c (diphosphate kinase), and Rv3418c (GroES), respectively. Newly appeared spots during depleted oxygen conditions in H37Rv were RvD 3, 4, 9,...”
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ETFA_SYNWW / Q0AZ33 Electron transfer flavoprotein subunit alpha from Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen) (see 2 papers)
SWOL_RS03520 electron transfer flavoprotein subunit alpha/FixB family protein from Syntrophomonas wolfei subsp. wolfei str. Goettingen G311
Swol_0697 electron transfer flavoprotein, alpha subunit from Syntrophomonas wolfei subsp. wolfei str. Goettingen
30% identity, 76% coverage
- function: Part of an electron transfer flavoprotein involved in syntrophic growth of S.wolfei with butyrate. Probably receives electrons from butyryl-CoA dehydrogenases, and transfers them to the membrane-bound quinone oxidoreductase Swol_0698.
cofactor: FAD (Binds 1 FAD per subunit.)
subunit: Heterodimer of an alpha and a beta subunit. - Membrane Complexes of Syntrophomonas wolfei Involved in Syntrophic Butyrate Degradation and Hydrogen Formation
Crable, Frontiers in microbiology 2016 - “...on crotonate ( Figure 1 ). In contrast, expression of the etfAB2 genes (SWOL_RS03515 and SWOL_RS03520) was not significantly changed under the three growth conditions ( Figure 1 ). FIGURE 1 Expression of hydABC2 (SWOL_RS09950, SWOL_RS09955 and SWOL_RS09960) (A) , etfAB2 (SWOL_RS03515 and SWOL_RS00520) and SWOL_RS03525...”
- “...dehydrogenase are transferred to the iron-sulfur (FeS) oxidoreductase (SWOL_RS03525 gene product) by (EtfAB2, SWOL_RS03515 and SWOL_RS03520). FeS oxidoreductase and an [FeFe]-hydrogenase form separate complexes in the membrane. Electrons from FeS oxidoreductase reduce menaquinone with protons consumed at outside surface of the membrane. The [FeFe]-hydrogenase oxidizes menaquinol...”
- Structural and Functional Changes in Prokaryotic Communities in Artificial Pit Mud during Chinese Baijiu Production
Liu, mSystems 2020 - “...fla 3.71 9.53* Clostridium tyrobutyricum Q0AVM3 Acetyl-CoA acetyltransferase Swol_1934 2.16 8.61* Syntrophomonas wolfei subsp. wolfei Q0AZ33 Electron transfer flavoprotein subunit alpha etfA 2.02 10.65* Syntrophomonas wolfei subsp. wolfei Q74IT6 Chaperone protein DnaK dnaK 0.37 0.21* Lactobacillus johnsonii Q7MVY0 Hydroxylamine reductase hcp 0.43 0.12* Porphyromonas gingivalis R7KAP8...”
- Proteomic analysis reveals metabolic and regulatory systems involved in the syntrophic and axenic lifestyle of Syntrophomonas wolfei
Sieber, Frontiers in microbiology 2015 - “...0.010 0.003 0.006 0.001 Swol_0696 Electron transfer flavoprotein -subunit 0.022 0.003 0.022 0.005 0.010 0.001 Swol_0697 Electron transfer flavoprotein -subunit 0.016 0.001 0.021 0.004 0.009 0.001 Swol_0767 Phosphate acetyltransferase 0.009 0.0003 0.010 0.001 0.008 0.0003 Swol_0768 Acetate kinase 0.007 0.001 0.013 0.002 0.008 0.001 Swol_1190 Molybdenum-pterin-binding...”
- “...interspecies hydrogen vs. formate transfer depends on growth condition. Proteins necessary for reverse electron transfer Swol_0697 and Swol_0696 gene products, which comprise the Etf complex EtfAB2, were among the most abundant proteins in the proteome in all growth conditions (Table 1 ; Figure 3 ), consistent...”
- A proteomic view at the biochemistry of syntrophic butyrate oxidation in Syntrophomonas wolfei
Schmidt, PloS one 2013 - “...2AB ), attributed the two previously identified genes [14] for electron-transfer flavoproteins EtfA and EtfB, Swol_0697 and Swol_0696, respectively ( Table 2 ). Notably, three sets of genes for EtfAB are encoded in the genome of S. wolfei [12] , [14] , however, the two identified...”
- Involvement of NADH:acceptor oxidoreductase and butyryl coenzyme A dehydrogenase in reversed electron transport during syntrophic butyrate oxidation by Syntrophomonas wolfei
Müller, Journal of bacteriology 2009 - “...paralog set of predicted etfBA genes (Swol_0696 and Swol_0697; no bcd candidate) makes it tempting to speculate that these genes might be coexpressed during...”
ATN24_RS08885 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium butyricum
34% identity, 72% coverage
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...B_methylotrophicum_DSM3468_GlcD_1 (BUME_04260), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04230), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24810), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2709), C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04240), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24820),...”
- “...NZ_CP013252 chromosome of C. butyricum KNU-L09 containing genes encoding EtfA (1: ATN24_RS03030, 2: ATN24_RS03165, 3: ATN24_RS08885) and EtfB (1: ATN24_RS03025, 2: ATN24_RS03160, 3: ATN24_RS08880), and potentially GlcD (1: ATN24_RS03035, 3: ATN24_RS08895, 4: ATN24_RS11095) A similar search was performed for the selected genomes of bacteria Roseburia intestinalis...”
M892_23815 electron transfer flavoprotein subunit alpha/FixB family protein from Vibrio campbellii ATCC BAA-1116
34% identity, 68% coverage
Q04QD6 Electron transfer flavoprotein, alpha subunit from Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
33% identity, 73% coverage
HMPREF0397_RS02340 electron transfer flavoprotein subunit alpha/FixB family protein from Fusobacterium nucleatum subsp. nucleatum ATCC 23726
33% identity, 77% coverage
- The Fused Methionine Sulfoxide Reductase MsrAB Promotes Oxidative Stress Defense and Bacterial Virulence in Fusobacterium nucleatum
Scheible, mBio 2022 - “...Histidine ammonia-lyase 6.57 HMPREF0397_RS02330 Acyl-CoA b dehydrogenase 6.57 HMPREF0397_RS02335 Electron transfer flavoprotein subunit beta 6.27 HMPREF0397_RS02340 Electron transfer flavoprotein subunit alpha 6.04 HMPREF0397_RS04970 Formate-tetrahydrofolate ligase 5.85 HMPREF0397_RS07365 ftcD Glutamate formimidoyltransferase 5.27 HMPREF0397_RS02930 Glu/Leu/Phe/Val dehydrogenase 5.16 HMPREF0397_RS07355 Formiminotetrahydrofolate cyclodeaminase 4.46 HMPREF0397_RS04270 Tyrosine phenol-lyase 4.35 HMPREF0397_RS07360 Imidazolonepropionase 4.27...”
LA_0411 electron transfer flavoprotein subunit alpha from Leptospira interrogans serovar lai str. 56601
LIC10360 electron transfer flavoprotein alpha-subunit from Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
32% identity, 73% coverage
- Extracellular proteome analysis of Leptospira interrogans serovar Lai
Zeng, Omics : a journal of integrative biology 2013 - “...study. Locus tag Name Gene COG LA_0222 LA_0411 OmpA family lipoprotein Electron transfer flavoprotein alpha subunit Putative lipoprotein LipL36 Hypothetical...”
- “...Pathway Proteins Locus tag Name Gene COG LA_0411 Electron transfer flavoprotein alpha subunit Putative lipoprotein 50S ribosomal protein L3 Thiol peroxidase...”
- Global proteome analysis of Leptospira interrogans
Eshghi, Journal of proteome research 2009 - “...protein PII LIC10440 0.8481.279 0.5010.568 1.526 NS NS NS 1.526 Electron transfer flavoprotein, alpha subunit LIC10360 0.9491.093 0.470.502 1.918 0.9561.245 0.5410.695 1.429 1.674 Electron transfer flavoprotein, beta subunit LIC10361 1.0151.065 0.4460.497 2.043 NS NS NS 2.043 Aconitate hydratase LIC20249 0.981.043 0.4150.423 2.314 0.851.317 0.3760.455 1.942 2.128...”
B9SA46 Electron transfer flavoprotein subunit alpha from Ricinus communis
30% identity, 76% coverage
CCS79_RS09600 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium diolis
34% identity, 71% coverage
- Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors
Detman, Microbial cell factories 2019 - “...C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2709), C_acetobutylicum_ATTC824_GlcD (CA_C2543), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03165), C_butyricum_KNU-L09_GlcD (ATN24_RS03030), C_butyricum_KNU-L09_LldP_GlcD (ATN24_RS08885), C_diolis_NJP7_3-hydroxybutyryl-CoA (CCS79_RS24290), C_diolis_NJP7_GlcD (CCS79_RS18270), C_diolis_NJP7_LldP_GlcD (CCS79_RS09600), C_kluyveri_DSM555_3-hydroxybutyryl-CoA (CKL_RS02260), C_kluyveri_DSM555_GlcD (CKL_RS17115), M_elsdenii_DSM20460 (MELS_RS10255), and M_elsdenii_DSM20460_acyl-CoA (MELS_RS10960). For EtfB: A_woodii_DSM1030_acyl-CoA (AWO_RS08100), A_woodii_DSM1030_GlcD_LldP (AWO_RS04410), B_methylotrophicum_DSM3468_acyl-CoA (BUME_07100), B_methylotrophicum_DSM3468_GlcD_1 (BUME_04270), B_methylotrophicum_DSM3468_GlcD_2 (BUME_04240), B_methylotrophicum_DSM3468_GlcD_LldP (BUME_24820), C_acetobutylicum_ATTC824_3-hydroxybutyryl-CoA (CA_C2710), C_acetobutylicum_ATTC824_GlcD (CA_C2544), C_butyricum_KNU-L09_3-hydroxybutyryl-CoA (ATN24_RS03160),...”
MAB_3362c Electron transfer flavoprotein alpha-subunit FixB from Mycobacterium abscessus ATCC 19977
33% identity, 77% coverage
NP_944591 electron transfer flavoprotein subunit alpha, mitochondrial from Danio rerio
32% identity, 76% coverage
PADG_07081 uncharacterized protein from Paracoccidioides brasiliensis Pb18
33% identity, 75% coverage
- Proteomic Analysis of Paracoccidioides brasiliensis During Infection of Alveolar Macrophages Primed or Not by Interferon-Gamma
Chaves, Frontiers in microbiology 2019 - “...PADG_06978, cytochrome C; PADG_04397, cytochrome c oxidase subunit 4, mitochondrial; PADG_05750, cytochrome c oxidase subunit; PADG_07081, electron transfer flavoprotein subunit alpha; PADG_11468, electron transfer flavoprotein beta-subunit; PADG_11981, V-type proton ATPase catalytic subunit A; PADG_04319, V-type ATPase, G subunit; PADG_03175, V-type proton ATPase subunit F; PADG_00688, F-type...”
- “...2.56 PADG_03175 V-type proton ATPase subunit F 1.87 2.88 PADG_08391 Plasma membrane ATPase ND 1.41 PADG_07081 Electron transfer flavoprotein subunit alpha ND 1.74 PADG_11468 Electron transfer flavoprotein beta-subunit PADG_06978 Cytochrome C ND 1.68 PADG_04397 Cytochrome c oxidase subunit 4, mitochondrial 2.88 3.55 PADG_05750 Putative cytochrome c...”
Q93615 Probable electron transfer flavoprotein subunit alpha, mitochondrial from Caenorhabditis elegans
32% identity, 75% coverage
I1KEH3 Electron transfer flavoprotein subunit alpha from Glycine max
32% identity, 63% coverage
PTH_1767 electron transfer flavoprotein, alpha subunit from Pelotomaculum thermopropionicum SI
34% identity, 72% coverage
EZN00_RS10865 electron transfer flavoprotein subunit alpha/FixB family protein from Clostridium tyrobutyricum
32% identity, 75% coverage
PTH_0017 electron transfer flavoprotein, alpha subunit from Pelotomaculum thermopropionicum SI
34% identity, 72% coverage
Swol_0266 hypothetical protein from Syntrophomonas wolfei subsp. wolfei str. Goettingen
34% identity, 77% coverage
- Stimulating Effect of Trichococcus flocculiformis on a Coculture of Syntrophomonas wolfei and Methanospirillum hungatei
Doloman, Applied and environmental microbiology 2022 - “...under tri-culture conditions: substrate binding protein of a TRAP-type transport system (Swol_0331), electron transfer flavoprotein (Swol_0266) and butyryl coenzyme A (butyryl-CoA) dehydrogenase (Swol_0268) ( Fig. 4A ; see also Table S1). Proteins Swol_0266 and Swol_0268 are in the same operon, while their isoforms, produced from genes...”
- “...dehydrogenase (Swol_0268) catalyzes conversion of butyryl-CoA to crotonyl-CoA and passes the electrons to electron-transfer flavoproteins (Swol_0266). The originally characterized butyryl-CoA dehydrogenase (Swol_2052) by Schmidt et al. ( 17 ) was also detected in the protein extract from our mid-exponential-phase grown cocultures, and its level of abundance...”
ETFA_ARATH / Q9C6I6 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
AT1G50940 ETFALPHA (electron transfer flavoprotein alpha); FAD binding / electron carrier from Arabidopsis thaliana
NP_175507 electron transfer flavoprotein alpha from Arabidopsis thaliana
29% identity, 75% coverage
- function: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF- ubiquinone oxidoreductase (ETF dehydrogenase). Involved in leucine catabolism and in phytol degradation (By similarity).
cofactor: FAD (Binds 1 FAD per dimer.)
subunit: Heterodimer of an alpha and a beta subunit - Identification of conserved drought-adaptive genes using a cross-species meta-analysis approach
Shaar-Moshe, BMC plant biology 2015 - “...Death loc_os03g05310 (AT3G44880, ACD1) Energy Mitochondrial electron transport Electron transfer flavoprotein loc_os04g10400 (AT5G43430, ETFBETA), loc_os03g61920 (AT1G50940 ETFALPHA) Cytochrome c reductase loc_os02g33730 (AT1G15120, ubiquinol-cytochrome C reductase complex 7.8kDa protein) Photosynthesis Light reaction and Calvin cycle loc_os01g12710 (AT4G13250, SDR family protein) loc_os07g05360 (AT1G79040, PSBR), loc_os11g47970 (AT2G39730, RCA), loc_os05g22614...”
- Identification of cleavage sites and substrate proteins for two mitochondrial intermediate peptidases in Arabidopsis thaliana
Carrie, Journal of experimental botany 2015 - “...Aldehyde dehydrogenase 2B Y YSNLAAAVENTITPPVKVEHTQLLIGGR SNLAAAVENTITPPVKVEHTQLLIGGR 3 AT1G32350 Alternative oxidase 1D L LSSDTSSPVSGNNQPENPIR SSDTSSPVSGNNQPENPIR 4 AT1G50940 Electron transfer flavoprotein alpha I ISISITSLSR SISITSLSR 5 AT2G14170 Aldehyde dehydrogenase 6B L LSTSPEQSTQPQMPPR STSPEQSTQPQMPPR 6 AT2G20360 NAD(P)-binding Rossmann-fold superfamily protein Y YSSSLATKGVGHLAR SSSLATKGVGHLAR 7 AT3G06050 Peroxiredoxin IIF F FSKLAEGTDITSAAPGVSLQKAR...”
- 2-Hydroxy Acids in Plant Metabolism
Maurino, The arabidopsis book 2015 - “...of and -subunits (the corresponding genes are At1g50940 and At5g43430, respectively). D-2HG accumulates in an Arabidopsis ETF -subunit loss-of-function mutant...”
- Proteomic analysis of endoplasmic reticulum stress responses in rice seeds
Qian, Scientific reports 2015 - “...gi|115465825 13.676 AT4G10960 UDP-glucose 4-epimerase 1 LOC_Os05g51670 gi|32490267 2.354 AT2G43180 carboxyvinyl-carboxyphosphonate phosphorylmutase LOC_Os04g42580 gi|115456435 2.048 AT1G50940 electron transfer flavoprotein subunit alpha LOC_Os03g61920 gi|115480417 3.038 AT5G66120 3-dehydroquinate synthase LOC_Os09g36800 gi|115436430 2.815 AT3G06860 3-hydroxyacyl-CoA dehydrogenase LOC_Os01g24680 gi|218188780 2.461 AT4G11030 AMP-binding enzyme LOC_Os01g46750 gi|213959137 3.561 no apical meristem protein...”
- Branched-Chain Amino Acid Metabolism in Arabidopsis thaliana
Binder, The arabidopsis book 2010 - “...been identified in mitochondria by mass spectrometry (ETF: At1g50940 and ETF: At5g43430) (Taylor et al., 2004). These proteins have not been specifically...”
- Searching for bidirectional promoters in Arabidopsis thaliana
Wang, BMC bioinformatics 2009 - “...MF zinc ion binding 19 AT2G19720 AT2G19730 30.9849 GO:0003735 MF structural constituent of ribosome 20 AT1G50940 AT1G50950 41.0419 GO:0009055 MF electron carrier activity 21 AT4G30680 AT4G30690 44.2758 GO:0003743 MF translation initiation factor activity 22 AT1G09080 AT1G09090 46.4263 GO:0009408 BP response to heat 23 AT1G65130 AT1G65140 52.4771...”
- ModuleFinder and CoReg: alternative tools for linking gene expression modules with promoter sequences motifs to uncover gene regulation mechanisms in plants
Holt, Plant methods 2006 - “...providing new avenues for NADH generation, or in the case of the electron transfer flavoprotein (At1g50940), provision of electrons to ubiquinone. Significantly, the new carbon substrates for these NADH generating pathways, while including the organic acids of the TCA cycle, are likely to be generated by...”
- The mitochondrial electron transfer flavoprotein complex is essential for survival of Arabidopsis in extended darkness.
Ishizaki, The Plant journal : for cell and molecular biology 2006 (PubMed)- GeneRIF: ETF-alpha has a role as part of the electron transfer flavoprotein complex in the catabolism of leucine and involvement in the chlorophyll degradation pathway activated during dark-induced carbohydrate deprivation.
PAAG_00173 electron transfer flavoprotein subunit alpha from Paracoccidioides lutzii Pb01
33% identity, 74% coverage
- Interacting with Hemoglobin: Paracoccidioides spp. Recruits hsp30 on Its Cell Surface for Enhanced Ability to Use This Iron Source
de, Journal of fungi (Basel, Switzerland) 2021 - “...Chaperone DnaK 2982.3 1.57 0.864 - PAAG_03292 Cytochrome c peroxidase, mitochondrial 3045.4 1.60 - 0.809 PAAG_00173 Electron transfer flavoprotein subunit alpha 465.5 1.88 - 0.642 PAAG_00850 Glutamine-fructose-6-phosphate transaminase (isomerizing) 1305.8 1.65 - 0.693 PAAG_00126 Histone H4.2 4337.0 1.62 0.792 - PAAG_00053 Malate dehydrogenase, NAD-dependent 1037.2 1.62...”
- Interaction of Isocitrate Lyase with Proteins Involved in the Energetic Metabolism in Paracoccidioides lutzii
Freitas, Journal of fungi (Basel, Switzerland) 2020 - “...alpha subunit 4619.729 Respiration PAAG_08037 ATP synthase subunit beta 4555.649 PAAG_00953 NADH-cytochrome b5 reductase 2806.097 PAAG_00173 electron transfer flavoprotein subunit alpha 3309.523 PAAG_02265 mitochondrial F1F0 ATP synthase subunit 14,297.79 Pentose-phosphate pathway PAAG_04166 transaldolase 12,361.49 PAAG_04444 transketolase 8237.422 Energy conversion and regeneration PAAG_03631 12-oxophytodienoate reductase * 4991.925...”
- “...polypeptide V 18,851.39 PAAG_06796 cytochrome c oxidase subunit 5b 18,717.38 PAAG_03292 cytochrome c peroxidase 13,367.75 PAAG_00173 electron transfer flavoprotein subunit alpha 23,108.45 PAAG_04931 electron transfer flavoprotein subunit beta 14,274.43 PAAG_02265 mitochondrial F1F0 ATP synthase subunit 16,924.33 PAAG_01078 alternative oxidase 15,371.76 Methylcitrate cycle PAAG_04549 mitochondrial 2-methylisocitrate lyase...”
- Transcriptional and proteomic responses to carbon starvation in Paracoccidioides
Lima, PLoS neglected tropical diseases 2014 - “...respiration PAAG_08088 cytochrome b-c1 complex subunit 2 # respiration PAAG_06268 cytochrome c # electron transport PAAG_00173 electron transfer flavoprotein subunit alpha # electron transport PAAG_03599 formate dehydrogenase # electron transport PAAG_01265 cytochrome b5 # electron transport PAAG_06796 cytochrome c oxidase polypeptide IV # electron transport Ethanol...”
VVA1024 electron transfer flavoprotein, alpha subunit from Vibrio vulnificus YJ016
32% identity, 74% coverage
XP_003714716 electron transfer flavoprotein subunit alpha from Pyricularia oryzae 70-15
32% identity, 74% coverage
Q7KN94 Electron transfer flavoprotein subunit alpha from Drosophila melanogaster
33% identity, 75% coverage
MSMEG_2352 electron transfer flavoprotein, alpha subunit from Mycobacterium smegmatis str. MC2 155
33% identity, 76% coverage
SPAC27D7.06 electron transfer flavoprotein alpha subunit from Schizosaccharomyces pombe
32% identity, 78% coverage
Dgeo_0720 electron transfer flavoprotein, alpha subunit from Deinococcus geothermalis DSM 11300
32% identity, 76% coverage
FE46_RS01775, FPSM_00371 electron transfer flavoprotein subunit alpha/FixB family protein from Flavobacterium psychrophilum
FP0335 Electron transfer flavoprotein, alpha subunit from Flavobacterium psychrophilum JIP02/86
30% identity, 75% coverage
- Dual RNA-Seq of Flavobacterium psychrophilum and Its Outer Membrane Vesicles Distinguishes Genes Associated with Susceptibility to Bacterial Cold-Water Disease in Rainbow Trout (Oncorhynchus mykiss)
Chapagain, Pathogens (Basel, Switzerland) 2023 - “...FE46_RS08120 4Fe-4S dicluster domain-containing 125.13 FE46_RS10110 Aconitate hydratase 59.32 FE46_RS10085 Cytochrome c oxidase accessory 56.74 FE46_RS01775 Electron transfer flavo subunit alpha family 40.12 FE46_RS12380 2-Oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase 37.85 FE46_RS01970 4Fe-4S dicluster domain-containing 33.28 FE46_RS12375 2-Oxoglutarate dehydrogenase E1 component 26.94 FE46_RS02175 L-glutamate gamma-semialdehyde dehydrogenase 25.98...”
- Stress Tolerance-Related Genetic Traits of Fish Pathogen Flavobacterium psychrophilum in a Mature Biofilm
Levipan, Frontiers in microbiology 2018 - “...protein FPSM_02567, smf GO:0003677, GO:0006281, GO:0009294 3.77 Electron transport FP0335 Electron transfer flavoprotein, alpha subunit FPSM_00371, etfA GO:0009055, GO:0050660 2.80 FP0336 Electron transfer flavoprotein, beta subunit FP0336 GO:0009055 3.16 FP0376 Alternative complex III, protein E precursor FPSM_00413, actE GO:0009055, GO:0020037 3.44 FP0377 Alternative complex III, protein...”
- Stress Tolerance-Related Genetic Traits of Fish Pathogen Flavobacterium psychrophilum in a Mature Biofilm
Levipan, Frontiers in microbiology 2018 - “...GO:0045454 2.76 FP2437 DNA processing Smf protein FPSM_02567, smf GO:0003677, GO:0006281, GO:0009294 3.77 Electron transport FP0335 Electron transfer flavoprotein, alpha subunit FPSM_00371, etfA GO:0009055, GO:0050660 2.80 FP0336 Electron transfer flavoprotein, beta subunit FP0336 GO:0009055 3.16 FP0376 Alternative complex III, protein E precursor FPSM_00413, actE GO:0009055, GO:0020037...”
U3J7F4 Electron transfer flavoprotein subunit alpha from Anas platyrhynchos platyrhynchos
31% identity, 77% coverage
- Dietary riboflavin supplementation improves meat quality, antioxidant capacity, fatty acid composition, lipidomic, volatilomic, and proteomic profiles of breast muscle in Pekin ducks
Tang, Food chemistry: X 2023 - “...U3IR48 Dihydrolipoyl dehydrogenase DLD 3.01 1.41 E03 R0K2W7 Glycerol-3-phosphate dehydrogenase 2 GPD2 6.21 2.51 E06 U3J7F4 Electron transfer flavoprotein alpha subunit ETFA 3.08 2.02 E04 TCA cycle U3IR48 Dihydrolipoyl dehydrogenase DLD 3.01 1.41 E03 U3IHF6 Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial SDHA 10.49 5.14 E06 U3J5X3...”
- Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducks
Wu, Journal of animal science and biotechnology 2022 - “...aldolase 0.58 3.1E-02 Electron transport chain U3I998 NDUFS1 NADH:ubiquinone oxidoreductase core subunit S1 0.76 3.7E-02 U3J7F4 ETFA Electron transfer flavoprotein alpha subunit 0.57 2.5E-02 R0LSV8 ETFDH Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (Fragment) 0.74 1.6E-03 TCA cycle U3IR48 DLD Dihydrolipoyl dehydrogenase 0.41 1.1E-03 U3ICQ7 DLAT Acetyltransferase component...”
- Maternal diet deficient in riboflavin induces embryonic death associated with alterations in the hepatic proteome of duck embryos
Tang, Nutrition & metabolism 2019 - “...3.05 7.07E-07 U3IFL0 Solute carrier family 25 member 20 SLC25A20 38.01 9 9 2.90 4.26E-04 U3J7F4 Electron transfer flavoprotein alpha subunit ETFA 62.87 17 17 2.69 1.46E-02 FAD U3J8W0 Acyl-CoA dehydrogenase short chain ACADS 49.30 14 14 2.36 4.32E-03 FAD U3IFB0 2,4-dienoyl-CoA reductase 1 DECR1 39.20...”
Gmet_2265 Electron transfer flavoprotein, alpha subunit from Geobacter metallireducens GS-15
31% identity, 76% coverage
XP_624102 electron transfer flavoprotein subunit alpha, mitochondrial from Apis mellifera
32% identity, 76% coverage
SCO1081 electron transfer flavoprotein, alpha subunit from Streptomyces coelicolor A3(2)
32% identity, 75% coverage
CNBG_5866 electron transfer flavoprotein alpha subunit from Cryptococcus deuterogattii R265
31% identity, 79% coverage
- Proteomic profiling of the influence of iron availability on Cryptococcus gattii
Crestani, Journal of proteome research 2012 - “...neoformans JEC21 3.9348E-123 94 CNBG_3210 XP_567624 endocytosis-related protein C. neoformans var. neoformans JEC21 0 85 CNBG_5866 Q5y223 electron transfer flavoprotein subunit alpha, mitochondrial C. gattii 2.1543E-158 98 CNBG_4946 XP_568254 translational initiation-related protein C. neoformans var. neoformans JEC21 0 97 CNBG_5765 XP_567951 14-3-3 protein C. neoformans var....”
CBU_1117 electron transfer flavoprotein, alpha subunit from Coxiella burnetii RSA 493
34% identity, 75% coverage
CNAG_06806 electron transfer flavoprotein alpha subunit from Cryptococcus neoformans var. grubii H99
32% identity, 76% coverage
- HDAC genes play distinct and redundant roles in Cryptococcus neoformans virulence
Brandão, Scientific reports 2018 - “...fusion protein 1.12944946 CNAG_05925 CDC3 Septin ring protein 0.208014522 CNAG_05970 PAK1 Ste/Ste20/PakA protein kinase 0.070285615 CNAG_06806 ETF1alpha Electron transfer flavoprotein alpha subunit 0.523135543 CNAG_06808 STE3alpha Pheromone a factor receptor 0.657125254 CNAG_06811 RPL22alpha Large subunit ribosomal protein L22e 0.406283818 CNAG_06812 SPO14alpha Phospholipase D1 0.323123919 CNAG_06813 CAP1alpha Hypothetical...”
- A Wor1-Like Transcription Factor Is Essential for Virulence of Cryptococcus neoformans
Paes, Frontiers in cellular and infection microbiology 2018 - “...GO:0006412 8.0929 0.0017488 CNAG_06809 IKS protein kinase Chr_05 204043 207446 GO:0043167, GO:0006464, GO:0016301 7.1915 0.0017488 CNAG_06806 ETF1 electron transfer flavoprotein alpha subunit Chr_05 212067 213908 + GO:0005739, GO:0043167, GO:0048856 7.82241 0.0017488 CNAG_07407 MF 3 fungal mating-type pheromone Chr_05 222703 223313 + #DIV/0! 0.0017488 CNAG_06971 MYO2 myosin...”
BPSL2499 electron transfer flavoprotein alpha-subunit from Burkholderia pseudomallei K96243
33% identity, 76% coverage
Q5Y223 Probable electron transfer flavoprotein subunit alpha, mitochondrial from Cryptococcus gattii serotype B (strain WM276 / ATCC MYA-4071)
30% identity, 78% coverage
- SWATH label-free proteomics analyses revealed the roles of oxidative stress and antioxidant defensing system in sclerotia formation of Polyporus umbellatus.
Li, Scientific reports 2017 - “...synthase subunit beta (Q24751) of complex V, and Alpha-ETF (electron transfer flavor protein subunit alpha, Q5Y223) were down regulated in IS to 0.58, 0.60, 0.61 and 0.65 folds relative to IH, respectively. Flavin adenine dinucleotide (FAD) synthase (Q6ING7) and cytochrome c oxidase subunit 6B-like protein (G2TRP6)...”
- “...Subunit beta (Q24751) of ATP synthase complex V and Alpha-ETF (electron transfer flavoprotein subunit alpha, Q5Y223) serving as specific electron acceptors were down-regulated. However, Cytochrome c oxidase subunit 6B-like protein of complex III and IV was increased at initial, developmental and mature phases in slcerotia which...”
- Proteomic profiling of the influence of iron availability on Cryptococcus gattii
Crestani, Journal of proteome research 2012 - “...JEC21 3.9348E-123 94 CNBG_3210 XP_567624 endocytosis-related protein C. neoformans var. neoformans JEC21 0 85 CNBG_5866 Q5y223 electron transfer flavoprotein subunit alpha, mitochondrial C. gattii 2.1543E-158 98 CNBG_4946 XP_568254 translational initiation-related protein C. neoformans var. neoformans JEC21 0 97 CNBG_5765 XP_567951 14-3-3 protein C. neoformans var. neoformans...”
XP_003700429 electron transfer flavoprotein subunit alpha, mitochondrial from Megachile rotundata
33% identity, 76% coverage
B5RI57 Electron transfer flavoprotein subunit alpha, mitochondrial (Fragment) from Salmo salar
31% identity, 76% coverage
- Candida utilis yeast as a functional protein source for Atlantic salmon (Salmo salar L.): Local intestinal tissue and plasma proteome responses
Reveco-Urzua, PloS one 2019 - “...72 kDa type IV collagenase precursor 0.076752 -3.7037 0.002394 B5DG39 L-lactate dehydrogenase 0.24712 -2.0167 0.02386 B5RI57 Electron-transfer-flavoprotein alpha polypeptide 12192 13.574 0.023921 B9ENN5 Complement C1q-like protein 4 0.24833 -2.0097 0.023926 C0HAL2 Elongation factor 1-alpha 4.026 0.023934 0.023934 C0HBQ3 Lymphocyte cytosolic protein 2 13144 13.682 0.023995 B5DG72...”
- “...72 kDa type IV collagenase precursor 0.077739 -3.6852 0.00245 A0A085U717 GMP/IMP nucleotidase 0.24972 -2.0016 0.02365 B5RI57 Electron-transfer-flavoprotein alpha polypeptide 135.16 7.0785 0.023767 C0HBQ3 Lymphocyte cytosolic protein 2 145.5 7.1849 0.023813 B5DG39 L-lactate dehydrogenase 0.25203 -1.9883 0.023831 C0HAI2 L-lactate dehydrogenase 0.0075714 -7.0452 0.023863 C0H808 Tubulin beta chain...”
Q7Q254 Electron transfer flavoprotein subunit alpha from Anopheles gambiae
31% identity, 76% coverage
DR0970 electron transfer flavoprotein, alpha subunit from Deinococcus radiodurans R1
33% identity, 76% coverage
BCAL2934 electron transfer flavoprotein alpha-subunit from Burkholderia cenocepacia J2315
34% identity, 76% coverage
F1N9U8 Electron transfer flavoprotein subunit alpha from Gallus gallus
31% identity, 75% coverage
TTHA1146 electron transfer flavoprotein, alpha subunit from Thermus thermophilus HB8
33% identity, 77% coverage
2a1uA / P13804 Crystal structure of the human etf e165betaa mutant (see paper)
30% identity, 77% coverage
- Ligand: flavin-adenine dinucleotide (2a1uA)
ETFA_HUMAN / P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 9 papers)
30% identity, 77% coverage
- function: Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:10356313, PubMed:15159392, PubMed:15975918, PubMed:27499296, PubMed:9334218). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed:9334218). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed:12815589, PubMed:1430199, PubMed:1882842).
cofactor: FAD (Binds 1 FAD per dimer.)
subunit: Heterodimer composed of ETFA and ETFB (PubMed:15159392, PubMed:15975918, PubMed:8962055, PubMed:9334218). Identified in a complex that contains ETFA, ETFB and ETFRF1 (PubMed:27499296). Interaction with ETFRF1 promotes dissociation of the bound FAD and loss of electron transfer activity (PubMed:27499296). Interacts with TASOR (By similarity). - N-terminal titin fragment: a non-invasive, pharmacodynamic biomarker for microdystrophin efficacy.
Boehler, Skeletal muscle 2024 - “...250.9 +/- 127.6 1752.3 +/- 1724.5 7.0 17,43543 SL008038 Electron transfer flavoprotein subunit alpha, mitochondrial P13804 274.0 +/- 92.1 1850.0 +/- 4174.9 6.8 294250 SL000396 Cytochrome c P99999 1101.8 +/- 759.7 6108.9 +/- 5064.5 5.5 Downregulated with disease 13,67140 SL000401 Neutrophil elastase P08246 20,227.6 +/- 35,004.5...”
- Proteomic analysis reveals changes in the proteome of human THP-1 macrophages infected with <i>Paracoccidioides brasiliensis</i>
de, Frontiers in cellular and infection microbiology 2023 - “...C9JRL4 Cytosolic malate dehydrogenase (Fragment) 13862.17 * P07954 Fumarate hydratase_ mitochondrial 2669.02 * Oxidative phosphorylation P13804 Electron transfer flavoprotein subunit alpha_ mitochondrial 1834.68 * C9J8T6 Cytochrome c oxidase copper chaperone 7210.97 * Q5SQT6 Inorganic diphosphatase 403.07 * H0Y9D8 Inorganic diphosphatase (Fragment) 1522.39 * Q15181 Inorganic pyrophosphatase...”
- Proteomic response of A549 lung cancer cell line to protein-polysaccharide complex Venetin-1 isolated from earthworm coelomic fluid
Czaplewska, Frontiers in molecular biosciences 2023 - “...protein, mitochondrial 0.02 1.73 13 P23588 EIF4B Eukaryotic translation initiation factor 4B 0.02 1.73 14 P13804 ETFA Electron transfer flavoprotein subunit alpha, mitochondrial 0.02 1.69 15 Q96IX5 ATP5MK ATP synthase membrane subunit DAPIT, mitochondrial 0.02 1.69 16 P31937 HIBADH 3-hydroxyisobutyrate dehydrogenase, mitochondrial 0.05 1.65 17 Q56VL3...”
- CLPX regulates mitochondrial fatty acid β-oxidation in liver cells.
Suzuki, The Journal of biological chemistry 2023 - “...DYNLL1 Dynein light chain 1, cytoplasmic 1 3 P30084 ECHS1 Enoyl-CoA hydratase, mitochondrial 7 29 P13804 ETFA Electron transfer flavoprotein subunit alpha, mitochondrial 5 8 P40939 HADHA Trifunctional enzyme subunit alpha, mitochondrial 10 17 P55084 HADHB Trifunctional enzyme subunit beta, mitochondrial 2 2 P50213 IDH3A Isocitrate...”
- Characterization of the pVHL Interactome in Human Testis Using High-Throughput Library Screening.
Falconieri, Cancers 2022 - “...Death-inducer obliterator 1 (DIDO1) Q9BTC0 tumor suppressor 3 Electron transfer flavoprotein subunit alpha, mitochondrial (ETFA) P13804 electron transport 3 26S proteasome regulatory subunit 4 (PSMC1) P62191 protein degradation 3 Histone deacetylase complex subunit (SAP30) O75446 deacetylation 3 E3 ubiquitin-protein ligase (TTC3) P53804 ubiquitination/protein degradation 3 Ankyrin...”
- Understanding the activating mechanism of the immune system against COVID-19 by Traditional Indian Medicine: Network pharmacology approach.
Thirumal, Advances in protein chemistry and structural biology 2022 - “...612003 Q6Y7W6 GFER GFER ENSG00000127554 4236 2671 600924 P55789 ETFA ETFA ENSG00000140374 3481 2108 608053 P13804 ANO6 ANO6 ENSG00000177119 25240 196527 608663 Q4KMQ2 GRPEL1 GRPEL1 ENSG00000109519 19696 80273 606173 Q9HAV7 CDK5RAP2 CDK5RAP2 ENSG00000136861 18672 55755 608201 Q96SN8 GTF2F2 GTF2F2 ENSG00000188342 4653 2963 189969 P13984 CEP112 CEP112...”
- Extracellular vesicles of human diabetic retinopathy retinal tissue and urine of diabetic retinopathy patients are enriched for the junction plakoglo bin protein.
Mighty, Frontiers in endocrinology 2022 - “...BSG P35613 Basigin 6.4E+07 3.5E+08 2.9E+08 METTL7A H0YI09 Methyltransferase-like protein 7A (Fragment) 5.1E+07 8.1E+08 ETFA P13804 Electron transfer flavoprotein subunit alpha, mitochondrial 4.8E+07 8.4E+07 COX4I1 P13073 Cytochrome c oxidase subunit 4 isoform 1, mitochondrial 4.4E+07 7.1E+07 ATP5F1 P24539 ATP synthase subunit b, mitochondrial 4.4E+07 1.5E+08 HSPA9...”
- The Expression of Rab8, Ezrin, Radixin and Moesin in the Ciliary Body of Cynomolgus Monkeys.
Tanabe, Juntendo Iji zasshi = Juntendo medical journal 2022 - “...0.00 1 68 Dystrophin P11532 426426.0 0.40 2 69 Electron transfer flavoprotein subunit alpha, mitochondrial P13804 35057.6 8.70 2 70 Elongation factor 1-alpha 1 P68104 50109.2 7.10 6 71 Endoplasmin P14625 92411.2 2.40 2 72 Endothelin-converting enzyme-like 1 O95672 87735.7 3.40 2 73 Eyes absent homolog...”
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BPHY_RS38485, Bphy_7803 electron transfer flavoprotein subunit alpha/FixB family protein from Paraburkholderia phymatum STM815
Bphy_7803 electron transfer flavoprotein alpha subunit from Burkholderia phymatum STM815
32% identity, 76% coverage
- Metabolomics and Dual RNA-Sequencing on Root Nodules Revealed New Cellular Functions Controlled by Paraburkholderia phymatum NifA
Bellés-Sancho, Metabolites 2021 - “...transporter permease Bphy_7645 3.49 BPHY_RS37045 ISL3 family transposase Bphy_7489 3.60 BPHY_RS37920 hypothetical protein Bphy_7681 3.62 BPHY_RS38485 electron transfer flavoprotein subunit alpha/FixB family protein Bphy_7803 fixB 3.63 BPHY_RS41535 IS3 family transposase 3.63 BPHY_RS36015 HyaD/HybD family hydrogenase maturation endopeptidase Bphy_7262 hybD 3.70 BPHY_RS36485 hypothetical protein 3.83 BPHY_RS41135 electron...”
- “...Bphy_7489 3.60 BPHY_RS37920 hypothetical protein Bphy_7681 3.62 BPHY_RS38485 electron transfer flavoprotein subunit alpha/FixB family protein Bphy_7803 fixB 3.63 BPHY_RS41535 IS3 family transposase 3.63 BPHY_RS36015 HyaD/HybD family hydrogenase maturation endopeptidase Bphy_7262 hybD 3.70 BPHY_RS36485 hypothetical protein 3.83 BPHY_RS41135 electron transfer flavoprotein alpha/beta-subunit Bphy_7804 3.90 BPHY_RS36645 alpha/beta fold...”
- Metabolomics and Transcriptomics Identify Multiple Downstream Targets of Paraburkholderia phymatum σ54 During Symbiosis with Phaseolus vulgaris
Lardi, International journal of molecular sciences 2018 - “...5.2 Bphy_7754 nitrogenase molybdenum-iron protein chain nifD 4.3 Bphy_7755 nitrogenase molybdenum-iron protein chain nifK 4.0 Bphy_7803 electron transfer flavoprotein subunit 3.5 Bphy_7804 electron transfer flavoprotein /-subunit 3.1 Inorganic ion transport and metabolism Bphy_0141 CutC family protein 3.2 Bphy_0257 ammonium transporter amtB 2.1 Bphy_1627 sulfate ABC transporter...”
Q88F97 Electron transfer flavoprotein subunit alpha from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
32% identity, 76% coverage
Mchl_1823 Electron transfer flavoprotein alpha subunit from Methylobacterium chloromethanicum CM4
31% identity, 76% coverage
ETFA_MOUSE / Q99LC5 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Mus musculus (Mouse) (see paper)
NP_663590 electron transfer flavoprotein subunit alpha, mitochondrial from Mus musculus
29% identity, 77% coverage
- function: Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF- ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.
cofactor: FAD (Binds 1 FAD per dimer.)
subunit: Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interaction with ETFRF1 promotes dissociation of the bound FAD and loss of electron transfer activity (By similarity). Interacts with TASOR (PubMed:31112734). - Hepatic neddylation targets and stabilizes electron transfer flavoproteins to facilitate fatty acid β-oxidation.
Zhang, Proceedings of the National Academy of Sciences of the United States of America 2020 - GeneRIF: Hepatic neddylation also enhances ETFA and ETFB expression in adult mice and prevents fasting-induced steatosis and mortality.
- Application of fluorescence two-dimensional difference in-gel electrophoresis as a proteomic biomarker discovery tool in muscular dystrophy research
Carberry, Biology 2013 - “...d, mitochondrial NP_082138 168 5.52 18,796 11 70 2.4 60 Electron transfer flavoprotein, subunit alpha NP_663590 195 8.62 35,336 8 39 2.5 61 Cofilin-2 NP_031714 158 7.66 18,814 5 36 2.5 62 Adenylate kinase, isoenzyme AK1 NP_067490 104 5.7 23,334 6 36 2.5 63 ATP synthase,...”
- Changes in protein fluxes in skeletal muscle during sequential stages of muscle regeneration after acute injury in male mice.
Bizieff, Scientific reports 2024 - “...Hemoglobin subunit beta 1 P02088 Extracellular 2.9870.566% 2.9330.685% 2.5980.668% 126 Electron transfer flavoprotein subunit alpha Q99LC5 OxPhos 8.7829.531% 9.2282.616% 4.5490.808% 127 Phosphate carrier protein Q8VEM8 Mitochondria 2.8241.441% 6.1193.707% 3.5482.193% Average % turnover per daystandard deviation of each individual protein in each group. Proteins with bold text...”
- Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis
Banfi, Antioxidants (Basel, Switzerland) 2023 - “...0.017 1.42 NEG Electron transfer flavoprotein subunit beta OS = Mus musculus GN = Etfb Q99LC5 9 0.011 1.42 NEG Electron transfer flavoprotein subunit alpha_ mitochondrial OS = Mus musculus GN = Etfa Q9CR62 2 0.003 1.41 NEG Mitochondrial 2-oxoglutarate/malate carrier protein OS = Mus musculus...”
- ISG15 Is Required for the Dissemination of Vaccinia Virus Extracellular Virions
Bécares, Microbiology spectrum 2023 - “...Slc25a5 0.82 6.00 P19783 Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Cox4i1 0.78 5.05 Q99LC5 Electron transfer flavoprotein subunit alpha, mitochondrial Etfa 0.74 2.95 P48962 ADP/ATP translocase 1 Slc25a4 0.73 6.00 Q9CZW5 Mitochondrial import receptor subunit TOM70 Tomm70 0.71 3.63 Q9D855 Cytochrome b-c 1 complex...”
- Adenosine-rich extract of Ganoderma lucidum: A safe and effective lipid-lowering substance.
Li, iScience 2022 - “...() O35459 Ech1 down () up: 316 () Q921G7 Etfdh down () up: 222 () Q99LC5 Etfa down () up: 206 () Q9R0H0 Acox1 down () up: 260 () up: 260 () Q921H8 Acaa1a down () up: 198 () ; down: 292 () Q8VCH0 Acaa1b down...”
- Mitochondrial Sirtuin-3 (SIRT3) Prevents Doxorubicin-Induced Dilated Cardiomyopathy by Modulating Protein Acetylation and Oxidative Stress.
Tomczyk, Circulation. Heart failure 2022 - “...enzyme subunit alpha Hadha Q8BMS1 KYESAYGTQFTPCQLLLDHANNSSK k K760 0.91 Electron transfer flavoprotein subunit alpha Etfa Q99LC5 LGGEVSCLVAGT k CDK K59 1.12 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Ndufa2 Q9CQ75 ECSEVQP k LWAR K64 1.15 Trifunctional enzyme subunit alpha Hadha Q8BMS1 FVDLYGAQ k VVDR K728...”
- Glycerophosphoinositol Promotes Apoptosis of Chronic Lymphocytic Leukemia Cells by Enhancing Bax Expression and Activation.
Boncompagni, Frontiers in oncology 2022 - “...disulfide-isomerase A6 Q9DC69 NADH dehydrogenase 1 alpha subcomplex subunit 9 Q9DB05 Alpha-soluble NSF attachment protein Q99LC5 Electron transfer flavoprotein subunit alpha, mitochondrial Q64674 Spermidine synthase Q9CR57 60S ribosomal protein L14 P35278 Ras-related protein Rab-5C P84099 60S ribosomal protein L19 P20108 Thioredoxin-dependent peroxide reductase, mitochondrial P61087 Ubiquitin-conjugating...”
- Proteomic Analysis of Cardiac Adaptation to Exercise by High Resolution Mass Spectrometry
Al-Menhali, Frontiers in molecular biosciences 2021 - “...protein S3 Rps3 242.7 16.5 0.0193 Q99LX0 Protein/nucleic acid deglycase DJ-1 Park7 493.9 74.9 0.0197 Q99LC5 Electron transfer flavoprotein subunit alpha, mitochondrial Etfa 223.7 16.5 0.0196 Q91VM9 Inorganic pyrophosphatase 2, mitochondrial Ppa2 394.2 43.7 0.0192 Q61941 NAD(P) transhydrogenase, mitochondrial Nnt 48.3 5.6 0.0188 P70670 Nascent polypeptide-associated...”
- MRL/MpJ tendon matrix-derived therapeutic promotes improved healing outcomes in scar-mediated canonical tendon healing.
Paredes, Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2021 - “...mitochondrial 23.3 Q542X7 Chaperonin subunit 2 (), isoform CRA_a 57.4 Q8BMK4 Cytoskeleton-associated protein 4 63.7 Q99LC5 Electron transfer flavoprotein subunit , mitochondrial 35 Q58E64 Elongation factor 1- 50.1 Q3UAD6 Endoplasmin 92.4 E9Q561 Eukaryotic initiation factor 4A-II 36.1 Q8BTM8 Filamin-A 281 P68040 Guanine nucleotide-binding protein subunit 2-like...”
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KYF39_09630 electron transfer flavoprotein subunit alpha/FixB family protein from Riemerella anatipestifer RA-YM
31% identity, 77% coverage
- Genome-Wide Analysis Reveals that PhoP Regulates Pathogenicity in Riemerella anatipestifer
Zhang, Microbiology spectrum 2022 - “...KYF39_08580 2.85 1.27E02 TonB-dependent receptor KYF39_08585 1.10 1.04E06 Alpha/beta hydrolase KYF39_08915 3.12 3.27E03 Hypothetical protein KYF39_09630 1.21 4.55E07 Electron transfer flavoprotein subunit alpha/FixB family protein TABLE2 DEGs directly upregulated by PhoP Gene Log 2 fold change P value Annotation KYF39_05585 1.02 2.19E03 Hypothetical protein KYF39_08995 2.03...”
- “...), an N -acetylmuramoyl- l -alanine amidase ( KYF39_04370 ), a FixB family protein ( KYF39_09630 ), LptD ( KYF39_04375 ), and serine peptidase ( KYF39_04790 ). The membrane proteins in bacteria are related to their pathogenicity and immunogenicity; for example, the lipopolysaccharide (LPS) assembly protein...”
SY28_RS10195 electron transfer flavoprotein subunit alpha/FixB family protein from Meiothermus taiwanensis
30% identity, 76% coverage
NMB2154 electron transfer flavoprotein, alpha subunit from Neisseria meningitidis MC58
33% identity, 77% coverage
NP_386750, SMc00728 PUTATIVE ELECTRON TRANSFER FLAVOPROTEIN ALPHA-SUBUNIT ALPHA-ETF FLAVOPROTEIN from Sinorhizobium meliloti 1021
33% identity, 68% coverage
NP_436007 probable EtfA2 electron-transport flavoprotein, alpha-subunit from Sinorhizobium meliloti 1021
33% identity, 68% coverage
- 'Ca. Liberibacter asiaticus' proteins orthologous with pSymA-encoded proteins of Sinorhizobium meliloti: hypothetical roles in plant host interaction
Kuykendall, PloS one 2012 - “...1E-128 electron transfer flavoprotein beta NP_435693.1 YP_003064781 4E-14 186 52 NP_435693 8E-18 electrontransfer flavoprotein beta NP_436007.1 YP_003064782 2E-77 316 69 NP_436007 1E-150 Electron transfer flavoprotein alpha NP_435692.1 YP_003064782 2E-20 245 48 NP_386750 3E-21 Electron transfer flavoprotein alpha NP_435691.1 YP_003065437 1E-18 326 43 NP_385139 6E-09 flavoprotein ubiquinone...”
- “...in energy production. YP_003064781 and YP_003064782, encoded by adjacent genes, are orthologs of NP_436008.1 and NP_436007.1 (E=3e-73 and 2e- 77 respectively). Both genes have two adjacent ortholog pairs on both pSymA and the S. meliloti chromosome. YP_003065174 and NP_435375.1 (E=3e-76) are annotated as energy yielding FAD-dependent...”
SO3144 electron transfer flavoprotein, alpha subunit from Shewanella oneidensis MR-1
30% identity, 77% coverage
ETFA_RAT / P13803 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Rattus norvegicus (Rat) (see paper)
29% identity, 77% coverage
- function: Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:7334008). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.
cofactor: FAD (Binds 1 FAD per dimer.)
subunit: Heterodimer composed of ETFA and ETFB (PubMed:7334008). Identified in a complex that contains ETFA, ETFB and ETFRF1. Interaction with ETFRF1 promotes dissociation of the bound FAD and loss of electron transfer activity (By similarity). Interacts with TASOR (By similarity). - Characterization of the flanking region of the Shiga toxin operon in Stx2a bacteriophages reveals a diversity of the NanS-p sialate O-acetylesterase gene.
Pascal, AIMS microbiology 2023 - “...Germany 2011 AB030484 Stx2a-O157-EDL933 HG803182.1 P13363 CB13363 O104:H4 Cucumis sativus Germany 2011 AB030484 Stx2a-O157-EDL933 HG792102.1 P13803 CB13803 O2:H27 bovine feces Germany 2011 AB030484 Stx2a-O157-EDL933 HG792105.1 P14437 CB14437 O104:H4 human Norway 2006 AB030484 Stx2a-O157-EDL933 HG792103.1 P8983 CB8983 O104:H4 human Germany 2001 AB030484 Stx2a-O157-EDL933 KU298437.1 phiON-2011 ON-2011 O104:H4...”
- The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity.
Ji, PloS one 2022 - “...D4A5F4 RGD1311575 Hypothetical LOC289568 1.70 0.013594 ECH1_RAT Q62651 Ech1 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial 1.69 0.006191 ETFA_RAT P13803 Etfa Electron transfer flavoprotein subunit alpha, mitochondrial 1.69 0.002644 G3V9U2_RAT G3V9U2 Acaa2 3-ketoacyl-CoA thiolase, mitochondrial 1.69 0.001865 D4A7X5_RAT D4A7X5 Ppm1k Protein phosphatase 1K (PP2C domain containing) (Predicted) 1.68 0.006727 KNG1_RAT...”
- Space Radiation-Induced Alterations in the Hippocampal Ubiquitin-Proteome System
Tidmore, International journal of molecular sciences 2021 - “...ijms-22-07713-t002_Table 2 Table 2 Titanium Exposure Marker (TEM) signature proteins. UniProt Accession Numbers F1LQ48 * P13803 Q07303 Q4KLM4 * O35763 P14604 Q02874 Q5M9I5 O70511 P18484 P62243 * Q63803 P09330 P21396 P53676 Q68FS2 * P13638 P26772 P48679 * Q91XU8 Fully mapped and annotated in UniProt. * Denotes...”
- Quantitative Proteomics Analysis for the Identification of Differential Protein Expression in Calf Muscles between Young and Old SD Rats Using Mass Spectrometry
Kim, ACS omega 2021 - “...(predicted) D4A9P9 3 vinculin A0A0G2K8V2 3.1 kelch-like protein41 Q9ER30 3.1 electron transfer flavoprotein subunit, mitochondrial P13803 3.1 Ckmt2 protein B0BNC0 3.1 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial P08461 3.2 3-mercaptopyruvate sulfurtransferase P97532 3.2 cadherin13 F1M7X3 3.4 -crystallinB chain P23928 3.4 -1-antiproteinase A0A0G2JY31 3.4 aldehyde...”
- “...Q920L2, B2RZ24, Q641Y2, Q68FT, P18163, P42123, Q64428, Q60587, Q5M9H2, P08461, Q66HF1, P85834, A2RRU1, P15650, G3V936, P13803, Q68FU3, Q5BK63, Q5RJN0, D4A0T0, Q68FR9, and P17764) presented the highest degree of connectivity, as shown in Figure 1 b. Figure 1 STRING interaction analysis of the differentially expressed genes. (a)...”
- Diverse Roles of Mitochondria in Renal Injury from Environmental Toxicants and Therapeutic Drugs.
Lash, International journal of molecular sciences 2021 - “...(NADP) (oxalosuccinate decarboxylase) 1.88 0.56 Q9ER34 Aconitate hydratase (aconitase) 2.54 0.32 Respiratory Electron Transport Chain P13803 Electron transfer flavoprotein subunit alpha 1.00 0.11 Q66HF1 NADH-ubiquinone oxidoreductase 75 kDa subunit 1.90 0.20 Amino Acid and Fatty Acid Metabolic Pathways P50442 Glycine amidotransferase (transamidinase) 1.92 0.15 Q64565 Alanine-glyoxylate...”
- Myocardial proteomic profile in pulmonary arterial hypertension.
Hołda, Scientific reports 2020 - “...specific acyl-CoA dehydrogenase, mitochondrial 1.09 Catalyzes the first step of mitochondrial fatty acid beta-oxidation pathway P13803 Etfa Electron transfer flavoprotein subunit alpha, mitochondrial 1.09 Mitochondrial fatty acid beta-oxidation pathway, amino acid metabolism Q02253 Aldh6a1 Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial 1.08 Valine and pyrimidine metabolism, binds fatty acyl-CoA...”
- Leucine-Rich Diet Modulates the Metabolomic and Proteomic Profile of Skeletal Muscle during Cancer Cachexia.
Cruz, Cancers 2020 - “...WL W P12075 Cytochrome c oxidase subunit 5B, mitochondrial GN = Cox5b >5 WL W P13803 Electron transfer flavoprotein subunit alpha, mitochondrial GN = Etfa 1.343 WL W P14408 Fumarate hydratase, mitochondrial GN = Fh >5 WL W P10860 Glutamate dehydrogenase 1, mitochondrial GN = Glud1...”
- Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs.
Martin, PloS one 2019 - “...separating the outgroup P . mirabilis from P . infestans . P . andina isolate P13803 grouped with haplotype I-6, -7, -8 and -9 in the network analysis; haplotype I-6 and I-7 are representatives of the HERB-1 genotype. With P . mirabilis as an outgroup there...”
- “...observed in any of the P . infestans isolates or the P . andina isolate (P13803) included in this study, but were present in the type culture of P . andina (P13365; Y. Zhang and M. Coffey, unpublished). The mitochondrial haplotypes for historical isolates recovered from...”
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Q2LIY7 Electron transfer flavoprotein subunit alpha from Periplaneta americana
30% identity, 76% coverage
ETFA_YEAST / Q12480 Probable electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF; Altered inheritance rate of mitochondria protein 45 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
NP_015329, YPR004C Aim45p from Saccharomyces cerevisiae
30% identity, 68% coverage
- function: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF- ubiquinone oxidoreductase (ETF dehydrogenase) (By similarity).
cofactor: FAD (Binds 1 FAD per dimer.)
subunit: Heterodimer of an alpha and a beta subunit. - Genome-Wide Transcription Study of Cryptococcus neoformans H99 Clinical Strain versus Environmental Strains
Movahed, PloS one 2015 - “...http://www.ncbi.nlm.nih.gov/blast/Blast.cgi-alnHdr_6321777 2.4 2.5 3.0 0.0199 CNAG_06239T0_1499 NP_013580.1 ERG13 Hydroxymethylglutaryl-CoA synthase 2.2 2.7 2.2 0.0151 CNAG_06806T0_862 NP_015329 AIM45 Probable electron transfer flavoprotein subunit alpha, mitochondrial 2.1 2.7 2.3 0.0078 CNAG_05784T1_1057 NP_012234 SSM4 E3 ubiquitin-protein ligase Doa10 2.1 2.0 2.2 0.0008 Down-regulated genes in H99 CNAG_05332T0_622 NP_012694 MPH2...”
- The flavoproteome of the yeast Saccharomyces cerevisiae
Gudipati, Biochimica et biophysica acta 2014 - “...I. mito. membr. cir2 YOR356W 15 Electron transferring flavoprotein FAD FAD_DHS (ETF_alpha) Mito. matrix aim45 YPR004C 16 1.5.99.8 Proline dehydrogenase FAD FAD_oxidored (Pro_dh) Mito. matrix put1 YLR142W 17 1.6.2.2 Cytochrome-b5 reductase FAD FAD_Lum_binding ER & o. mito. membr. cbr1 YIL043C (FAD_binding_6) ER & plasma membr. pga3...”
- Characterization of chromosome stability in diploid, polyploid and hybrid yeast cells
Kumaran, PloS one 2013 - “...YCL009C (8837 bp; A) YCR004C (3183 bp; T) 292.023.9 Chromosome XVI YPL001W (228 bp; T) YPR004C (7934 bp;T) 1.160.19 Chromosome XVI YPL003W (2549 bp; T) YPR004C (7934 bp;T) 1.200.25 Some of our marked chromosomes have markers inserted very near to and transcribing towards the centromere (see...”
- Flavin adenine dinucleotide rescues the phenotype of frataxin deficiency
Gonzalez-Cabo, PloS one 2010 - “...not in mitochondria; however, homologous genes for ETF complex genes have been reported in yeast: ypr004c as the ETF homologue and ygr207c as the homologue of ETF. We have also demonstrated that Yfh1p interacts with two of these components of the electron transfer flavoprotein complex [15]...”
- “...wild-type strain (W303) and in the single mutants yfh1 , sdh1 , sdh2 , and ypr004c (the yeast homologue of the mammalian electron transfer flavoprotein gene, ETF ). The frataxin mutant showed a significantly decreased activity in every complex except for complex I ( Figure 5A...”
- Global analysis of the relationship between the binding of the Bas1p transcription factor and meiosis-specific double-strand DNA breaks in Saccharomyces cerevisiae
Mieczkowski, Molecular and cellular biology 2006 - “...3.10 0.4866 0.4541 0.6396 0.4917 5.64E01 5.16E01 NS NS YPR004C YPR004C 2.65 0.3017 0.1206 3.04E01 NS MNT4 YNR059W 2.37 0.3273 0.2185 4.50E01 NS GID8 GAT2...”
- Genetic and environmental factors influencing glutathione homeostasis in Saccharomyces cerevisiae
Perrone, Molecular biology of the cell 2005 - “...relative to the parent. Interestingly, deletion of YPR004c, encoding a putative electron transfer flavoprotein that localizes to the mitochondria, where it...”
- Structural and Functional Characterization of an Electron Transfer Flavoprotein Involved in Toluene Degradation in Strictly Anaerobic Bacteria.
Vogt, Journal of bacteriology 2019
Avin_16420 Electron transfer flavoprotein, alpha subunit from Azotobacter vinelandii AvOP
31% identity, 75% coverage
- Azotobacter Genomes: The Genome of Azotobacter chroococcum NCIMB 8003 (ATCC 4412)
Robson, PloS one 2015 - “...identity to orthologs in Av-DJ. These include undecaprenyl-phosphate galactosephosphotransferase (Avin_16230), the eex D,E,F gene cluster (Avin_16420 to 16460) and the transcriptional activator, rfaH (Achr_26880; Avin_15910). All the other genes show highest identity to genes from various Pseudomonads, other -proteobacteria, -proteobacteria and even cyanobacteria. A cluster of...”
c2094 Putative electron transfer flavoprotein subunit ydiR from Escherichia coli CFT073
30% identity, 72% coverage
NP_459833 putative electron transfer flavoprotein alpha subunit from Salmonella typhimurium LT2
32% identity, 75% coverage
PA2951 electron transfer flavoprotein alpha-subunit from Pseudomonas aeruginosa PAO1
PA14_25880 electron transfer flavoprotein alpha-subunit from Pseudomonas aeruginosa UCBPP-PA14
32% identity, 69% coverage
- The Temperature-Regulation of Pseudomonas aeruginosa cmaX-cfrX-cmpX Operon Reveals an Intriguing Molecular Network Involving the Sigma Factors AlgU and SigX
Bouffartigues, Frontiers in microbiology 2020 - “...number PA number Protein name Putative function Fold change CS/Control Standard deviation Energy metabolism 1 PA2951 EtfA Electron transfer flavoprotein, alpha subunit 2.33 0.3 2 PA0552 Pgk 3-phosphoglycerate kinase 3.93 0.16 3 PA2953 EtfD Electron transfer flavoprotein-ubiquinone oxidoreductase 2.85 0.5 Amino acid biosynthesis and metabolism 4...”
- General and condition-specific essential functions of Pseudomonas aeruginosa
Lee, Proceedings of the National Academy of Sciences of the United States of America 2015 - “...(31). Remarkably, however, three general essential genes (PA2951- PA2953) were identified whose (mitochondrial) homologs function in respiration to transfer...”
- A shotgun antisense approach to the identification of novel essential genes in Pseudomonas aeruginosa
Rusmini, BMC microbiology 2014 - “...several bacterial species [ 20 ]. Six intragenic fragments derived from PA4669 ( ipk ), PA2951 ( etfA ), PA3687 ( ppc ), PA3758 ( nagA ), PA1183 ( dctA ), and PA1805 ( ppiD ), which are homologous to genes previously shown to be essential...”
- “...- isopentenyl monophosphate kinase(2) Biosynthesis of cofactors, prosthetic groups and carriers Vc, Se, Pa G2 PA2951 etfA - electron transfer flavoprotein alpha-subunit (2) Energy metabolism Ab, Cc H2 S10F8 PA5186 probable iron-containing alcohol dehydrogenase (3) Energy metabolism F1 PA1554 ccoN1 \ fixN \ Cytochrome c oxidase,...”
- Identification of five structurally unrelated quorum-sensing inhibitors of Pseudomonas aeruginosa from a natural-derivative database
Tan, Antimicrobial agents and chemotherapy 2013 - “...PA3922 PA3919 PA2300 PA1372 PA0572 PA0792 PA0400 PA5213 PA2951 PA0586 PA2399 PA3924 PA2290 PA2424 PA3148 PA2302 piv, prpL hutU PA2402 PA0852 PA3083 NA PA0588...”
- A theoretical and experimental proteome map of Pseudomonas aeruginosa PAO1
Lecoutere, MicrobiologyOpen 2012 - “...PA2851 efp Translation elongation factor P J C 21.0 27 4.82 4.85 0.702 0.283 73 PA2951 etfA Electron transfer flavoprotein C U 31.4 34 4.98 4.96 0.843 0.343 74 PA2965 fabF1 Betaketoacylacyl carrier synthase IQ C 43.5 45 5.62 5.64 0.654 0.034 75 PA2967 fabG Oxoacylacyl...”
- Genomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa
Dötsch, Antimicrobial agents and chemotherapy 2009 - “...PA2645 PA2647d PA2647d PA2691 PA2693 PA2700 PA2797 PA2871 PA2951 PA2953 PA2955 PA2970 PA2989 PA3014 PA3015 PA3016 PA3047 PA3063 PA3093 PA3127 PA3141 PA3170...”
- Immunoproteomics to examine cystic fibrosis host interactions with extracellular Pseudomonas aeruginosa proteins
Upritchard, Infection and immunity 2008 - “...proteins 1 2 5 PA no.c Protein FliC FliC BraC 7 PA2951 EtfA 8 11 20 21 23 PA0974 PA0277 PA0634 PA0624 PA4385 PA0974 SpeB PA0634 PA0624 GroEL PA0423 PA2939...”
- Induction by cationic antimicrobial peptides and involvement in intrinsic polymyxin and antimicrobial peptide resistance, biofilm formation, and swarming motility of PsrA in Pseudomonas aeruginosa
Gooderham, Journal of bacteriology 2008 - “...12, 2017 by University of California, Berkeley PA0506 PA2673 PA2951 PA2952 PA2953 PA3013 PA3014 PA3622 Gene name 5630 J. BACTERIOL. GOODERHAM ET AL. TABLE 4....”
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- Genomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa
Dötsch, Antimicrobial agents and chemotherapy 2009 - “...PA14_29320 PA14_29290 PA14_29220 PA14_27950 PA14_26960 PA14_25880 PA14_25840 PA14_25820 PA14_25630 PA14_25420 PA14_25080 PA14_25060 PA14_25050 PA14_24690...”
YdiR / b1698 putative electron transfer flavoprotein subunit YdiR from Escherichia coli K-12 substr. MG1655 (see 3 papers)
b1698 predicted electron transfer flavoprotein, FAD-binding from Escherichia coli str. K-12 substr. MG1655
29% identity, 72% coverage
Q5F5I8 Electron transfer flavoprotein subunit beta from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
NGO1936 EtfA from Neisseria gonorrhoeae FA 1090
32% identity, 77% coverage
ZMO1479 Electron transfer flavoprotein alpha subunit from Zymomonas mobilis subsp. mobilis ZM4
31% identity, 67% coverage
- Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032
Yang, Biotechnology for biofuels 2018 - “...A (L174F) 14989271498927 ZMO1470 G to A (A277V) 14994161499416 ZMO1470 G to A (P114L) 15092991509299 ZMO1479 ( etfA ) G to A (A48V) 15195831519583 ZMO1485 C to T (D284D) 15429001542900 ZMO1511 G to A (R130K) 15727651572765 ZMO1535 C to T (S9F) 15850591585059 ZMO1544 ( cobS )...”
- Transcriptome profiling of Zymomonas mobilis under ethanol stress
He, Biotechnology for biofuels 2012 - “...b (ZMO0957), cytochrome c 1 (ZMO0958), cytochrome c -type biogenesis proteins (ZMO1252-1254), electron transfer flavoprotein (ZMO1479 and ZMO1480), NADH dehydrogenase ( ndh , ZMO1113), NADH:flavin oxidoreductase (ZMO1885), NADH:ubiquinone oxidoreductase complex (ZMO1809-ZMO1811), fumarate reductase (ZMO0569). Hayashi et al. previously isolated respiratory-deficient mutant (RDM) strains of Z. mobilis...”
- The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4
Seo, Nature biotechnology 2005 - “...b (ZMO0957), cytochrome c 1 (ZMO0958), cytochrome c -type biogenesis proteins (ZMO12521256), electron transfer flavoprotein (ZMO1479, ZMO1480) and a ubiquinone biosynthesis protein (ZMO1189, ZMO1669). Genes for electron donor and receptor modules such as NADH dehydrogenase (ZMO1113) NADH:flavin oxidoreductase (ZMO1885), NADH:ubiquinone oxidoreductase complex (ZMO18091814), nitroreductase (ZMO0678) and...”
NMA0241 electron transfer flavoprotein alpha-subunit from Neisseria meningitidis Z2491
32% identity, 75% coverage
YP_002636467 putative electron transfer flavoprotein alpha subunit from Salmonella enterica subsp. enterica serovar Paratyphi C strain RKS4594
32% identity, 75% coverage
NP_001121188 electron transfer flavoprotein subunit alpha, mitochondrial isoform b from Homo sapiens
31% identity, 64% coverage
PTH_0597 electron transfer flavoprotein, alpha subunit from Pelotomaculum thermopropionicum SI
33% identity, 69% coverage
XALc_2699 probable electron transfer flavoprotein alpha subunit from Xanthomonas albilineans
27% identity, 75% coverage
XTGART29_0580 electron transfer flavoprotein subunit alpha/FixB family protein from Xanthomonas graminis pv. graminis ART-Xtg29
31% identity, 63% coverage
ETFA_METME / P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
AAA64953.1 electron transfer flavoprotein large subunit from Methylophilus methylotrophus W3A1 (see paper)
29% identity, 75% coverage
- function: Heterodimeric electron transfer flavoprotein that accepts electrons from trimethylamine dehydrogenase (PubMed:7798207). It transfers the electrons to the main respiratory chain via ETF- ubiquinone oxidoreductase (ETF dehydrogenase) (Probable).
cofactor: FAD (Binds 1 FAD per dimer.)
subunit: Heterodimer of an alpha and a beta subunit.
BQ09680 Electron transfer flavoprotein alpha-subunit from Bartonella quintana str. Toulouse
30% identity, 71% coverage
A3WEN4 Electron transfer flavoprotein beta-subunit:Electron transfer flavoprotein, alpha subunit from Erythrobacter sp. NAP1
30% identity, 75% coverage
XF0253 electron transfer flavoprotein alpha subunit from Xylella fastidiosa 9a5c
28% identity, 75% coverage
1efpA / P38974 Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
33% identity, 62% coverage
- Ligand: flavin-adenine dinucleotide (1efpA)
3clrD / P53571 Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
29% identity, 75% coverage
- Ligand: flavin-adenine dinucleotide (3clrD)
XAC3587 electron transfer flavoprotein alpha subunit from Xanthomonas axonopodis pv. citri str. 306
31% identity, 53% coverage
H0YK49 Electron transfer flavoprotein subunit alpha from Homo sapiens
32% identity, 53% coverage
BOV_RS09295 electron transfer flavoprotein subunit alpha/FixB family protein from Brucella ovis ATCC 25840
BAB1_1971 Antifreeze protein, type I:Electron transfer flavoprotein, alpha subunit from Brucella melitensis biovar Abortus 2308
32% identity, 62% coverage
Dshi_0216 Electron transfer flavoprotein alpha subunit from Dinoroseobacter shibae DFL 12
33% identity, 63% coverage
BPI_II555 electron transfer flavoprotein subunit alpha/FixB family protein from Brucella pinnipedialis B2/94
31% identity, 62% coverage
TGME49_007250, TGME49_207250 electron-transfer-flavoprotein, alpha polypeptide from Toxoplasma gondii ME49
30% identity, 65% coverage
swp_4940 FixB protein from Shewanella piezotolerans WP3
28% identity, 69% coverage
- Condition-Specific Molecular Network Analysis Revealed That Flagellar Proteins Are Involved in Electron Transfer Processes of Shewanella piezotolerans WP3
Ding, Genetics research 2021 - “...swp_3875, swp_3458, swp_0182 2 9 0.50 9.74 E 04 Oxidative phosphorylation swp_4058, swp_0854, swp_4352, swp_3589, swp_4940, swp_1424, swp_1425, swp_0430, swp_0429 3 6 0.87 1.26 E 03 Flagellar assembly swp_1531, swp_3616, swp_1536, swp_1521, swp_1527, swp_1525 4 4 0.67 1.01 E 02 swp_0679, swp_4217, swp_0678, swp_2705 5 4...”
- “...swp_4652, swp_4657, swp_4655 7 7 0.52 2.19 E 02 swp_2623, swp_2495, swp_4933, swp_4301, swp_4936, swp_4935, swp_4940 8 4 0.50 2.89 E 02 Purine metabolism swp_1361, swp_4724, swp_2972, swp_2621 9 5 0.50 4.13 E 02 NrfD family swp_4653, swp_4654, swp_4652, swp_4655, swp_1240 10 6 0.53 4.29 E...”
Q39UY1 Electron transfer flavoprotein, alpha subunit from Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
29% identity, 70% coverage
PMI2652 electron transfer flavoprotein alpha subunit for carnitine metabolism from Proteus mirabilis HI4320
30% identity, 71% coverage
Swol_2121 electron transfer flavoprotein alpha subunit from Syntrophomonas wolfei subsp. wolfei str. Goettingen
29% identity, 64% coverage
YaaR / b0042 putative electron transfer flavoprotein FixB from Escherichia coli K-12 substr. MG1655 (see 7 papers)
FIXB_ECOLI / P31574 Protein FixB from Escherichia coli (strain K12) (see paper)
b0042 predicted electron transfer flavoprotein, NAD/FAD-binding domain and ETFP adenine nucleotide-binding domain-like from Escherichia coli str. K-12 substr. MG1655
28% identity, 68% coverage
SPA0077 FixB protein from Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150
28% identity, 69% coverage
SSO2762 Electron transfer flavoprotein, subunit alpha (etfA) from Sulfolobus solfataricus P2
30% identity, 65% coverage
PFREUD_02490 electron transfer flavoprotein subunit alpha/FixB family protein from Propionibacterium freudenreichii subsp. shermanii CIRM-BIA1
45% identity, 28% coverage
F1SJX1 Electron transfer flavoprotein subunit alpha from Sus scrofa
26% identity, 77% coverage
Dred_0573 electron transfer flavoprotein, alpha subunit from Desulfotomaculum reducens MI-1
30% identity, 72% coverage
Gmet_2152 Electron transfer flavoprotein, alpha subunit from Geobacter metallireducens GS-15
Q39TP3 Electron transfer flavoprotein, alpha subunit from Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
30% identity, 57% coverage
Saci_0291 electron transfer flavoprotein from Sulfolobus acidocaldarius DSM 639
31% identity, 60% coverage
D0CBH8 Electron transfer flavoprotein subunit alpha from Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81)
30% identity, 76% coverage
AOLE_04180 electron transfer flavoprotein subunit alpha/FixB family protein from Acinetobacter oleivorans DR1
29% identity, 76% coverage
- Endogenous hydrogen peroxide increases biofilm formation by inducing exopolysaccharide production in Acinetobacter oleivorans DR1
Jang, Scientific reports 2016 - “...previous data suggested that oprC is an OxyR-controlled gene 23 and that EtfA (encoded by AOLE_04180), which is involved in electron transport, and FumC (encoded by AOLE_07235) are highly upregulated by H 2 O 2 and superoxide generators, respectively. OxyR is a master regulator of oxidative...”
- “...34.7 6.8 dho dehydrogenase AOLE_09905 H 204 37.9 4.3 etfA electron transfer flavoprotein subunit alpha AOLE_04180 H 5605 62.2 5.5 gabD NADP-dependent aldehyde dehydrogenase AOLE_06655 16.2 1804 92.2 4.7 fepA TonB-dependent receptor AOLE_09880 9.4 1904 104 4.7 cirA TonB dependent receptor family protein AOLE_02770 8.7 3304...”
5ow0A / Q39VG4 Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
29% identity, 58% coverage
- Ligand: flavin-adenine dinucleotide (5ow0A)
Q39VG4 Electron transfer flavoprotein, alpha subunit from Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
Gmet_1526 Electron transfer flavoprotein, alpha subunit from Geobacter metallireducens GS-15
29% identity, 58% coverage
- Adaptation of Carbon Source Utilization Patterns of Geobacter metallireducens During Sessile Growth
Marozava, Frontiers in microbiology 2020 - “...3.9 Q39VG2 Benzoylsuccinyl-CoA thiolase subunit 4.9 13.3 13.0 Q39VG1 Benzoylsuccinyl-CoA thiolase subunit 4.6 3.2 4.5 Q39VG4 Electron transfer flavoprotein, alpha subunit 3.9 1.7 1.5 Q39VG8 Succinyl:(R)-benzylsuccinate coenzyme A transferase subunit 3.5 5.0 4.0 Q39VG5 Electron transfer flavoprotein, beta subunit 3.5 1.5 1.4 Q39VG6 (E)-2-benzylidenesuccinyl-CoA hydratase 3.5...”
- Genome sequence of the deltaproteobacterial strain NaphS2 and analysis of differential gene expression during anaerobic growth on naphthalene
DiDonato, PloS one 2010 - “...a putative naphthyl-2-methyl-succinyl-CoA dehydrogenase, were both upregulated on naphthalene, as was NPH_4694, a homolog to Gmet_1526, encoded by a gene adjacent to the bns gene cluster in NaphS2. In the soluble naphthalene-grown fraction, NPH_5898, a 3-hydroxybutyryl-CoA dehydratase, NPH_5903, a conserved hypothetical protein, and NPH_5908, an acyl-CoA...”
- “...second contig contains the genes bbsCDEFGH , followed by orthologs to the G. metallireducens genes Gmet_1526, Gmet_1527, and Gmet_1528, which in the G. metallireducens genome are found in the toluene degradation cluster downstream of the bbs operon, and preceding bssCAB . Gmet_1526 and Gmet_1527 are similar...”
ETFA_CHRSD / Q1QYV9 Electron transfer flavoprotein subunit alpha; Alpha-ETF from Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11) (see paper)
28% identity, 76% coverage
- function: Participates in the electron transfer process during N,N- dimethylglycine (DMG) degradation to sarcosine.
cofactor: FAD (Binds 1 FAD per dimer.)
subunit: Heterodimer of an alpha and a beta subunit.
disruption phenotype: Deletion mutant loses its ability to grow using DMG as the sole nitrogen source but is still capable of utilizing sarcosine and glycine as the sole nitrogen source.
YgcQ / b2769 putative flavoprotein from Escherichia coli K-12 substr. MG1655 (see paper)
29% identity, 61% coverage
S2978 putative flavoprotein from Shigella flexneri 2a str. 2457T
29% identity, 61% coverage
FN1424 ACYL-COA dehydrogenase, short-chain specific from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
35% identity, 23% coverage
H0YL83 Electron transfer flavoprotein subunit alpha from Homo sapiens
49% identity, 17% coverage
W5PKE2 Electron transfer flavoprotein subunit alpha from Ovis aries
25% identity, 68% coverage
Acfer_1575 acyl-CoA dehydrogenase domain protein from Acidaminococcus fermentans DSM 20731
36% identity, 20% coverage
- Complete genome sequence of Acidaminococcus fermentans type strain (VR4)
Chang, Standards in genomic sciences 2010 - “...(Acfer_1756) is encoded at the beginning of the gene cluster. Three acyl-CoA dehydrogenase genes (Acfer_1477, Acfer_1575 and Acfer_1583) were annotated at various locations, completing the pathway. Nevertheless, genes encoding 2-hydroxyglutarate dehydrogenase and Butyl-CoA:acetate CoA transferase have not yet been identified. Possibly these enzymes have additional functions...”
- “...decarboxylase subunit r Acfer_1836 glutaconyl-CoA decarboxylase sodium pump, subunit r Acfer_1477 acyl-CoA dehydrogenase domain protein Acfer_1575 acyl-CoA dehydrogenase domain protein Acfer_1583 acyl-CoA dehydrogenase domain protein Enzymes of -lactamase and the related Acfer_0250 -lactamase domain-containing protein Acfer_0522 Zn-dependent hydrolase of the -lactamase fold Acfer_0551 RNA-metabolizing metallo--lactamase Acfer_0879...”
BCAM2321 putative electron transfer flavoprotein alpha subunit from Burkholderia cenocepacia J2315
26% identity, 70% coverage
BTH_II1863 electron transfer flavoprotein, subunit alpha, putative from Burkholderia thailandensis E264
36% identity, 28% coverage
HCDSEM_125 putative electron transfer flavoprotein, alpha subunit from Candidatus Hodgkinia cicadicola Dsem
32% identity, 30% coverage
AEQU_2232 electron transfer flavoprotein subunit alpha/FixB family protein from Adlercreutzia equolifaciens DSM 19450
34% identity, 30% coverage
PP0312, PP_0312 electron transfer flavoprotein, alpha subunit from Pseudomonas putida KT2440
34% identity, 29% coverage
PA5400 probable electron transfer flavoprotein alpha subunit from Pseudomonas aeruginosa PAO1
24% identity, 78% coverage
- The effect of antibacterial peptide ε-Polylysine against Pseudomonas aeruginosa biofilm in marine environment
Jiang, 2024 - Role of N,N-Dimethylglycine and Its Catabolism to Sarcosine in Chromohalobacter salexigens DSM 3043
Yang, Applied and environmental microbiology 2020 (secret) - Choline Catabolism in Burkholderia thailandensis Is Regulated by Multiple Glutamine Amidotransferase 1-Containing AraC Family Transcriptional Regulators
Nock, Journal of bacteriology 2016 - “...gbcA-B, the dgc operon (PA5376, PA5377, dgcA, dgcB, PA5400, and PA5401), and the sarcosine oxidase genes, which together contribute to the sequential...”
- Understanding the antimicrobial mechanism of TiO₂-based nanocomposite films in a pathogenic bacterium
Kubacka, Scientific reports 2014 - “...8.1-fold; co III, 6.1-fold; PA0107 , 6.7-fold; PA0111 , 8.3-fold), an electron transfer flavoprotein ( PA5400 , 6.5-fold) and two oxidoreductases ( adh A, 14.3-fold; dgc A, 9.4-fold). These results provide evidence of the selective effect of TiO 2 photocatalysis on distinct components of the respiratory...”
- Small-molecule inhibition of choline catabolism in Pseudomonas aeruginosa and other aerobic choline-catabolizing bacteria
Fitzsimmons, Applied and environmental microbiology 2011 - “...- and -subunits of an electron transfer protein (PA5400 and PA5401). In Chromohalobacter salexignes and the Betaproteobacteria, the predicted dgc genes are...”
- “...and -subunits of an electron transfer protein encoded by PA5400 and PA5401 in P. aeruginosa; dgcA, dgcB, gbcA, gbcB, soxG, glyA1, and gbdR all represent...”
- Characterization of temporal protein production in Pseudomonas aeruginosa biofilms
Southey-Pillig, Journal of bacteriology 2005 - “...energy ............................................................................................................PA5400 Phosphopantetheine...”
- “...subunit ......................................................................................................................................PA5400 Alkyl...”
- Construction of a mini-Tn5-luxCDABE mutant library in Pseudomonas aeruginosa PAO1: a tool for identifying differentially regulated genes
Lewenza, Genome research 2005 - “...50 F5 and regulate the expression of genes 20 PA5400 Electron transfer flavoprotein 11 G6 necessary for resistance to cationic anti10 PA4359 feoA Fe2+ transport...”
Z4079 No description from Escherichia coli O157:H7 EDL933
38% identity, 20% coverage
- Clonal and antigenic analysis of serogroup A Neisseria meningitidis with particular reference to epidemiological features of epidemic meningitis in the People's Republic of China
Wang, Infection and immunity 1992 - “...Z4062, Z4065, Z4066, Z4069, Z4070, Z4071, Z4073, Z4075, Z4079, Z4081, Z4097, Z4104, Z4109, Z4115, Z4736, Z4737, Z4738, Z4739, Z4740, Z4744, Z4745, Z4747, Z4748,...”
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory