PaperBLAST
PaperBLAST Hits for BRENDA::Q0ZB96 serine O-acetyltransferase (EC 2.3.1.30) (Klebsiella pneumoniae) (273 a.a., MPCEELDIVW...)
Show query sequence
>BRENDA::Q0ZB96 serine O-acetyltransferase (EC 2.3.1.30) (Klebsiella pneumoniae)
MPCEELDIVWNNIKAEARALADCEPMLASFYHATLLKHENLGSALSYMLANKLASPIMPA
IAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPLLYLKGFHALQAYRIGHWL
WTQGRRALAIFLQNQVSVSFQVDIHPAAKIGRGIMLDHATGIVVGETAVIEDDVSILQSV
TLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGAKIGAGSVVLQPVPPHTTAAGVP
ARIVGKPESDKPAMDMDQHFNGIHHTFEYGDGI
Running BLASTp...
Found 231 similar proteins in the literature:
Q0ZB96 serine O-acetyltransferase (EC 2.3.1.30) from Klebsiella pneumoniae (see paper)
100% identity, 100% coverage
GM298_12115 serine O-acetyltransferase from Enterobacter sp. HSTU-ASh6
98% identity, 100% coverage
CYSE_SALTY / P29847 Serine acetyltransferase; SAT; Serine transacetylase; EC 2.3.1.30 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
P29847 serine O-acetyltransferase (EC 2.3.1.30) from Salmonella enterica subsp. enterica serovar Typhimurium (see paper)
NP_462599 serine acetyltransferase from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
98% identity, 100% coverage
CUC46_19530 serine O-acetyltransferase from Citrobacter freundii
96% identity, 100% coverage
- Citrobacter freundii fitness during bloodstream infection
Anderson, Scientific reports 2018 - “...a FC (Adj. P) ruvA CUC46_10495 Holliday junction processing protein 39.1 (8.410 5 ) cysE CUC46_19530 L-serine- O -acetyltransferase 10.3 (6.610 4 ) nlpI CUC46_17375 lipoprotein 8.5 (2.710 5 ) tatC CUC46_20805 twin-arginine translocase 5.5 (<1.010 10 ) mtlD CUC46_19485 mannitol-1-phosphate 5-dehydrogenase 4.8 (<1.010 10 )...”
CysE / b3607 serine acetyltransferase (EC 2.3.1.30) from Escherichia coli K-12 substr. MG1655 (see 4 papers)
cysE / P0A9D4 serine acetyltransferase (EC 2.3.1.30) from Escherichia coli (strain K12) (see 16 papers)
CYSE_ECOLI / P0A9D4 Serine acetyltransferase; SAT; EC 2.3.1.30 from Escherichia coli (strain K12) (see paper)
P0A9D4 serine O-acetyltransferase (EC 2.3.1.30) from Escherichia coli (see 4 papers)
cysE / RF|NP_418064 serine acetyltransferase from Escherichia coli K12 (see 3 papers)
b3607 serine acetyltransferase from Escherichia coli str. K-12 substr. MG1655
NP_418064 serine acetyltransferase from Escherichia coli str. K-12 substr. MG1655
NP_709386 serine acetyltransferase from Shigella flexneri 2a str. 301
96% identity, 100% coverage
- catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
subunit: Homohexamer; dimer of a homotrimer (PubMed:10617639). Forms a cysteine synthase complex with 2 copies of CysK (By similarity). - Quantitative assessment of thermodynamic constraints on the solution space of genome-scale metabolic models
Hamilton, Biophysical journal 2013 - “...b2818 argA Lethal Lethal Nonlethal Lethal Nonlethal Lethal b3607 cysE Lethal Lethal Nonlethal Lethal Nonlethal Lethal b3608 gpsA Lethal Lethal Nonlethal Lethal...”
- Genome-scale analysis to the impact of gene deletion on the metabolism of E. coli: constraint-based simulation approach
Xu, BMC bioinformatics 2009 - “...b2020 b2750, b2751 b3941 b3608 b2021, b2022 b2752, b2762 b2023, b2024 b2763, b2764 b2025, b2026 b3607 SS IITM OP U genes b3040 b3731, b3732 s0001 b3196 b3733, b3734 s0001 b3735, b3736 b3737, b3738 * genes (text in bold) are important but not essential genes We also...”
- Time-dependent translational response of E. coli to excess Zn(II)
Easton, Journal of biomolecular techniques : JBT 2006 (secret) - Experimental and computational assessment of conditionally essential genes in Escherichia coli
Joyce, Journal of bacteriology 2006 - “...(b1693) aroE (b3281) cysC (b2750) cysD (b2752) cysE (b3607) cysH (b2762) cysI (b2763) cysJ (b2764) cysK (b2414) cysN (b2751) cysP (b2425) [cysQ (b4214)] glyA...”
- Restricting Promiscuity of Plant Flavonoid 3'-Hydroxylase and 4'-O-Methyltransferase Improves the Biosynthesis of (2S)-Hesperetin in E. coli
Liu, Journal of agricultural and food chemistry 2023 - “...pGH21. Furthermore, the coding sequences of homoserine succinyltransferase (metA; NP_418437), l -serine O -acetyltransferase (cysE; NP_418064), and methionine adenosyltransferase (metK; WP001062128) from E. coli , as well as ydaO (BAA19269) from B. sutbilis were subcloned into pRSFDuet-1, resulting in plasmids pGH22pGH26. To assemble the (2 S...”
- Modulation of Escherichia coli serine acetyltransferase catalytic activity in the cysteine synthase complex.
Benoni, FEBS letters 2017 - GeneRIF: Data show that bacterial serine acetyltransferase (CysE) is activated when bound to O-acetylserine sulfhydrylase-A sulfhydrylase (CysK).
- On the interaction site of serine acetyltransferase in the cysteine synthase complex from Escherichia coli.
Zhao, Biochemical and biophysical research communications 2006 (PubMed)- GeneRIF: C-terminus of serine acetyltransferase, Ile 273, along with Glu 268 & Asp 271, was found to be essential for complex formation. Effects of O-acetyl-L-serine & sulfide on affinity for complex formation were also studied using surface plasmon technique.
- Role of CysE in production of an extracellular signaling molecule in Providencia stuartii and Escherichia coli: loss of CysE enhances biofilm formation in Escherichia coli.
Sturgill, Journal of bacteriology 2004 - GeneRIF: required for the production of an extracellular signal molecule. CysE mutant formed biofilms at an accelerated rate compared to the wild type.
- Is perturbation in the quaternary structure of bacterial CysE, another regulatory mechanism for cysteine synthesis?
Verma, International journal of biological macromolecules 2018 (PubMed)- GeneRIF: This study discovers that V241 variant of S. flexneri CysE has nearly null enzymatic activity. The observation is explained by molecular dynamic studies which reveal that the disorder generated by A241V substitution is the basis of dissociation of the quaternary assembly of S. flexneri CysE leading to loss of enzymatic activity and stability.
1t3dA / P0A9D4 Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
96% identity, 96% coverage
YPO0070 serine acetyltransferase from Yersinia pestis CO92
YPTB0066 serine acetyltransferase from Yersinia pseudotuberculosis IP 32953
86% identity, 100% coverage
3gvdI / A0A2U2H3H7 Crystal structure of serine acetyltransferase cyse from yersinia pestis
86% identity, 99% coverage
ETAE_3456 serine acetyltransferase from Edwardsiella tarda EIB202
83% identity, 100% coverage
PMI3185 serine acetyltransferase from Proteus mirabilis HI4320
PMI_RS15750 serine O-acetyltransferase from Proteus mirabilis HI4320
81% identity, 100% coverage
VC2649 serine acetyltransferase from Vibrio cholerae O1 biovar eltor str. N16961
74% identity, 97% coverage
PM1430 CysE from Pasteurella multocida subsp. multocida str. Pm70
76% identity, 94% coverage
4h7oA / Q9KNT2 Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
75% identity, 93% coverage
- Ligands: cysteine; arginine (4h7oA)
VF_2347 serine O-acetyltransferase from Aliivibrio fischeri ES114
VF_2347 serine acetyltransferase from Vibrio fischeri ES114
72% identity, 97% coverage
HSM_0020 serine acetyltransferase from Haemophilus somnus 2336
76% identity, 92% coverage
P43886 serine O-acetyltransferase (EC 2.3.1.30) from Haemophilus influenzae (see paper)
74% identity, 93% coverage
HD0659 serine acetyltransferase from Haemophilus ducreyi 35000HP
71% identity, 99% coverage
1ssqD / P43886 Serine acetyltransferase- complex with cysteine (see paper)
74% identity, 92% coverage
Asuc_0384 serine O-acetyltransferase from Actinobacillus succinogenes 130Z
75% identity, 92% coverage
U876_22910 serine O-acetyltransferase from Aeromonas hydrophila NJ-35
75% identity, 91% coverage
MS2212 CysE protein from Mannheimia succiniciproducens MBEL55E
MS2212 serine O-acetyltransferase from [Mannheimia] succiniciproducens MBEL55E
73% identity, 92% coverage
MMSR116_RS25615 serine O-acetyltransferase from Methylobacterium mesophilicum SR1.6/6
59% identity, 95% coverage
RSP_2481 serine acetyltransferase from Rhodobacter sphaeroides 2.4.1
57% identity, 93% coverage
- Convergence of the transcriptional responses to heat shock and singlet oxygen stresses
Dufour, PLoS genetics 2012 - “...Other RSP_1825 Protein folding and stabilization RSP_1207 Ribosomal proteins: synthesis and modification RSP_0570 Serine family RSP_2481 Fatty acid and cell envelope Biosynthesis RSP_0473 Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides RSP_2569 Other RSP_3601 DNA metabolism DNA replication, recombination, and repair RSP_2388, RSP_2966 Central intermediary metabolism...”
NGFG_01496, NGFG_RS07905 serine O-acetyltransferase from Neisseria gonorrhoeae MS11
Q5F6X0 Serine acetyltransferase from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
57% identity, 92% coverage
- Serine acetyltransferase from Neisseria gonorrhoeae; structural and biochemical basis of inhibition
Oldham, The Biochemical journal 2022 - “...for expression in Escherichia coli The SAT gene (annotated cysE ) NGFG_RS07905 (old locus tag NGFG_01496) from N. gonorrhoeae MS11 was codon optimized for E. coli and ordered from Geneart (Thermo Fisher). The synthetic NGFG_RS07905 construct was cloned into expression vector pET28b between Ndel and Xhol...”
- “...novel drug target for treating extensively antimicrobial resistant gonorrhoea. Associated content UniProt Accession ID for NGFG_01496 (NgSAT): Q5F6X0 (Q5F6X0_NEIG1) Acknowledgment We thank Professor Vic Arcus and Dr. Claire Mulholland for help and advice regarding solving the structure of NgSAT. This research made use of the SAXS...”
- “...methods Cloning of NgSAT for expression in Escherichia coli The SAT gene (annotated cysE ) NGFG_RS07905 (old locus tag NGFG_01496) from N. gonorrhoeae MS11 was codon optimized for E. coli and ordered from Geneart (Thermo Fisher). The synthetic NGFG_RS07905 construct was cloned into expression vector pET28b...”
- “...Results and discussion Purification and stoichiometry of NgSAT The N. gonorrhoeae serine acetyltransferase (SAT) gene NGFG_RS07905 was cloned into pET28b for expression with an N-terminal His-tag. This was done to avoid adding residues onto the C-terminus of the protein which is proposed to interact with OASS/CysK...”
- Serine acetyltransferase from Neisseria gonorrhoeae; structural and biochemical basis of inhibition
Oldham, The Biochemical journal 2022 - “...target for treating extensively antimicrobial resistant gonorrhoea. Associated content UniProt Accession ID for NGFG_01496 (NgSAT): Q5F6X0 (Q5F6X0_NEIG1) Acknowledgment We thank Professor Vic Arcus and Dr. Claire Mulholland for help and advice regarding solving the structure of NgSAT. This research made use of the SAXS and MX2...”
6wyeA / Q5F6X0 Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
57% identity, 92% coverage
- Ligand: (2s)-2-hydroxybutanedioic acid (6wyeA)
C4IRW0 serine O-acetyltransferase (EC 2.3.1.30) from Brucella abortus (see paper)
52% identity, 96% coverage
CCNA_02734 serine acetyltransferase from Caulobacter crescentus NA1000
53% identity, 90% coverage
BOV_1224 serine acetyltransferase 4 (atsat-4) (atserat3;2) from Brucella ovis ATCC 25840
51% identity, 99% coverage
4hzdA / A0A0F6AR69 Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
54% identity, 89% coverage
- Ligands: coenzyme a; magnesium ion (4hzdA)
Dshi_2161 serine O-acetyltransferase from Dinoroseobacter shibae DFL 12
54% identity, 93% coverage
A0A0C5DAX6 serine O-acetyltransferase (EC 2.3.1.30) from Vitis vinifera (see paper)
52% identity, 70% coverage
4n69A / I1KHY6 Soybean serine acetyltransferase complexed with serine (see paper)
56% identity, 88% coverage
LOC107804363 serine acetyltransferase 2-like from Nicotiana tabacum
50% identity, 70% coverage
- Alternative Oxidase Inhibition Impairs Tobacco Root Development and Root Hair Formation
Liu, Frontiers in plant science 2021 - “...several DEGs (|log 2 FC 1|) were commonly enriched in these pathways, i.e., LOC107808059 and LOC107804363, which encode serine acetyltransferase, were enriched in the KEGG pathways of Carbon metabolism, Biosynthesis of amino acids, and Cysteine and methionine metabolism, and showed upregulation on SHAM treatment ( Figures...”
A8JDD3 serine O-acetyltransferase from Chlamydomonas reinhardtii
50% identity, 52% coverage
SAT1 / Q42588 serine acetyltransferase (EC 2.3.1.30) from Arabidopsis thaliana (see 4 papers)
SAT1_ARATH / Q42588 Serine acetyltransferase 1, chloroplastic; AtSAT-1; AtSERAT2;1; SAT-p; EC 2.3.1.30 from Arabidopsis thaliana (Mouse-ear cress) (see 9 papers)
Q42588 serine O-acetyltransferase (EC 2.3.1.30) from Arabidopsis thaliana (see 7 papers)
CAA84371.1 serine acetyltransferase from Arabidopsis thaliana (see paper)
NP_175988 serine acetyltransferase 2;1 from Arabidopsis thaliana
AT1G55920 ATSERAT2;1 (SERINE ACETYLTRANSFERASE 2;1); serine O-acetyltransferase from Arabidopsis thaliana
52% identity, 81% coverage
- function: Serine acetyltransferase which catalyzes the formation of O- acetyl-L-serine from acetyl-CoA and L-serine (PubMed:7851429, PubMed:9830017). Also displays O-acetylserine (thio1)-lyase activity in vitro (PubMed:7851429). May be involved in detoxification process by mediating the production of glutathione.
catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
subunit: Homomultimer (By similarity). Interacts with OASA1 and CYP20- 3. Component of the cysteine synthase complex (CSC) composed of two OAS-TL dimers and one SAT hexamer. - Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume.
Tabe, Journal of experimental botany 2010 - GeneRIF: Expression in developing lupin embryos of a serine acetyltransferase (SAT) from Arabidopsis thaliana (AtSAT1 or AtSerat 2;1) was associated with increases of up to 5-fold in the concentrations of O-acetylserine and up to 26-fold in free cysteine.
- Analysis of cytosolic and plastidic serine acetyltransferase mutants and subcellular metabolite distributions suggests interplay of the cellular compartments for cysteine biosynthesis in Arabidopsis.
Krueger, Plant, cell & environment 2009 (PubMed)- GeneRIF: Cytosolic SERAT1.1 and plastidic SERAT2.1 do not contribute to cysteine biosynthesis.
- Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis.
Watanabe, The Plant cell 2008 - GeneRIF: Plastidic SERAT2;1 contributes less to OAS formation and subsequent Cys synthesis.
- Thermodynamics of the interaction between O-acetylserine sulfhydrylase and the C-terminus of serine acetyltransferase.
Kumaran, Biochemistry 2007 (PubMed)- GeneRIF: Binding of the C-terminus of serine acetyltransferase by acetylserine sulfhydrylase contributes a large portion of the total binding energy during formation of the plant cysteine synthase complex.
- In silico assessment of gene function involved in cysteine biosynthesis in Arabidopsis: expression analysis of multiple isoforms of serine acetyltransferase.
Noji, Amino acids 2006 (PubMed)- GeneRIF: From the results of sensitivity to feedback regulation, subcellular localization and expression of mRNA analyses, each serine acetyltransferase (SATase) isoform seems to have its specific role for cysteine biosynthesis.
- New insights into the regulation of plant metabolism by O-acetylserine: sulfate and beyond
Apodiakou, Journal of experimental botany 2023 - “...and Nikiforova, 2008 ). In Arabidopsis five genes encoding SERAT proteins can be found: SERAT2;1 (AT1G55920) and SERAT2;2 (AT3G13110), which are localized in plastids and mitochondria, respectively, and SERAT1;1 (AT5G56760), SERAT3;1 (AT2G17640), and SERAT3;2 (AT4G35640), which are localized and expressed in the cytosol ( Watanabe et...”
- “...( Fig. 2 ; Table 1 ). Of the five SERAT genes, SERAT1;1 (At5g56760), SERAT2;1 (At1g55920), SERAT2;2 (At3g13110), SERAT3;1 (At2g17640), and SERAT3;2 (At4g35640), each has a different transcriptional response to certain conditions ( Watanabe and Hoefgen, 2017 ). It was shown that SERAT group III genes...”
- HYPK promotes the activity of the Nα-acetyltransferase A complex to determine proteostasis of nonAc-X2/N-degron-containing proteins
Miklánková, Science advances 2022 - “...lowering its NTA frequency and not by a pleiotropic effect. The non-NatA substrates MPP SAT5 (AT1G55920) and MRE TUBB4 (AT5G44340) ( Fig. 6, A and B ) were stable in hypk mutants, suggesting that the enhanced global protein turnover in hypk was predominantly triggered by destabilization...”
- Cotranslational N-degron masking by acetylation promotes proteome stability in plants
Linster, Nature communications 2022 - “...OAS-TL A interacting protein MPP SAT5 ( S erine- A cetyl- T ransferase 5 , AT1G55920) and MED COI1 ( CO ronatine- I nsensitive protein 1 , AT2G39940) were not destabilized in NatA depleted plants (Fig. 2d , Supplementary Fig. 3d ). Since the steady-state level...”
- The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and Inhibits Lepidium sativum Seed Germination via Multiple Mechanisms including Interference with Auxin Homeostasis
Nakabayashi, International journal of molecular sciences 2022 - “...Oxidoreductase 6.4 43.5 UP UP UP - - - UP UP UP UP DN UP AT1G55920 SAT1 4.7 5.6 UP UP - UP - UP UP UP UP UP - UP AT5G39050 PMAT1 2.0 2.6 UP UP UP UP UP UP UP - UP UP -...”
- Identification of Key Metabolic Pathways and Biomarkers Underlying Flowering Time of Guar (Cyamopsis tetragonoloba (L.) Taub.) via Integrated Transcriptome-Metabolome Analysis
Grigoreva, Genes 2021 - “...0.009 nd AT2G30970 (ASP1); AT5G22300 (NIT4) 0.386; 0.533 0.097; 0.006 C00065 (L-Serine) 0.393 0.008 0.001 AT1G55920 (ATSERAT2;1) 1.448 5.492 10 5 genes-12-00952-t005_Table 5 Table 5 Description of differentially expressed guar transcripts involved in the phosphatidylinositol signaling pathway based on homology with Arabidopsis thaliana on KEGG map...”
- Expression of cyanobacterial genes enhanced CO2 assimilation and biomass production in transgenic Arabidopsis thaliana
Abbasi, PeerJ 2021 - “...involved in oxidative photosynthetic pathway, specifically synthesis of glycerate biosynthesis. 1.03 Timm et al., 2011 AT1G55920 Serine acetyltransferase Involved in cysteine biosynthesis in chloroplasts and cytosol. 1.1 Tavares et al., 2015 AT5G50950 Fumarase It is reportedly located in chloroplasts, cytosol and mitochondria. It is involved in...”
- pH-dependent transcriptional profile changes in iron-deficient Arabidopsis roots
Tsai, BMC genomics 2020 - “...ENDOTRANSGLUCOSYLASE/HYDROLASE 17 0.49 2.95 0.17 AT2G32150 Haloacid dehalogenase-like hydrolase (HAD) superfamily protein 0.49 3.13 0.16 AT1G55920 SAT1, SERINE ACETYLTRANSFERASE 1 0.36 2.56 0.14 AT2G39380 EXO70H2, EXOCYST SUBUNIT EXO70 FAMILY PROTEIN H2 0.39 2.78 0.14 AT4G38540 FAD/NAD(P)-binding oxidoreductase family protein 0.40 2.88 0.14 AT3G11340 UGT76B1, UDP-DEPENDENT GLYCOSYLTRANSFERASE...”
- Chemical Priming by Isothiocyanates Protects Against Intoxication by Products of the Mustard Oil Bomb
Ferber, Frontiers in plant science 2020 - “...five cysteine forming enzymes of the serine acetyltransferase (SAT) gene family: SERAT1;1 (At5g56760, SAT-c), SERAT2;1 (At1g55920, SAT-p), SERAT2;2 (At3g13110, SAT-m), SERAT3;1 (At2g17640, SAT-106) and SERAT3;2 (At4g35640). Except for SERAT2;1 which was found to be induced by 1.6-fold by SF 4 h after treatment, none of these...”
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Q6IWF8 serine O-acetyltransferase (EC 2.3.1.30) from Noccaea goesingensis (see paper)
53% identity, 79% coverage
sat / CAD67560.1 serine O-acetyltransferase from Beta vulgaris (see paper)
52% identity, 88% coverage
SAT52 / Q42538 serine acetyltransferase (EC 2.3.1.30) from Arabidopsis thaliana (see 4 papers)
SAT5_ARATH / Q42538 Serine acetyltransferase 5; AtSAT-5; AtSERAT1;1; SAT-c; EC 2.3.1.30 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q42538 serine O-acetyltransferase (EC 2.3.1.30) from Arabidopsis thaliana (see 4 papers)
NP_200487 serine acetyltransferase 1;1 from Arabidopsis thaliana
AT5G56760 ATSERAT1;1 (ARABIDOPSIS THALIANA SERINE ACETYLTRANSFERASE 1;1); serine O-acetyltransferase from Arabidopsis thaliana
54% identity, 79% coverage
- catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
subunit: Homomultimer. - Photorespiration: regulation and new insights on the potential role of persulfidation
Aroca, Journal of experimental botany 2023 - “...P (3) AT3G13110 Q39218 SAT3 Serine acetyltransferase 3, mitochondrial P (3) , S (5,7) AT5G56760 Q42538 SAT5 Serine acetyltransferase 5 P (3) AT5G04590 Q9LZ66 SIR Assimilatory sulfite reductase (ferredoxin) chloroplastic P (3,4) , S (6,7) AT1G79230 O64530 STR1 Thiosulfate/3-mercaptopyruvate sulfurtransferase 1 mitochondrial P (3,4) , S...”
- Analysis of cytosolic and plastidic serine acetyltransferase mutants and subcellular metabolite distributions suggests interplay of the cellular compartments for cysteine biosynthesis in Arabidopsis.
Krueger, Plant, cell & environment 2009 (PubMed)- GeneRIF: Cytosolic SERAT1.1 and plastidic SERAT2.1 do not contribute to cysteine biosynthesis.
- Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis.
Watanabe, The Plant cell 2008 - GeneRIF: SERAT1;1 may play a major role during seed development.
- In silico assessment of gene function involved in cysteine biosynthesis in Arabidopsis: expression analysis of multiple isoforms of serine acetyltransferase.
Noji, Amino acids 2006 (PubMed)- GeneRIF: From the results of sensitivity to feedback regulation, subcellular localization and expression of mRNA analyses, each serine acetyltransferase (SATase) isoform seems to have its specific role for cysteine biosynthesis.
- New insights into the regulation of plant metabolism by O-acetylserine: sulfate and beyond
Apodiakou, Journal of experimental botany 2023 - “...SERAT2;1 (AT1G55920) and SERAT2;2 (AT3G13110), which are localized in plastids and mitochondria, respectively, and SERAT1;1 (AT5G56760), SERAT3;1 (AT2G17640), and SERAT3;2 (AT4G35640), which are localized and expressed in the cytosol ( Watanabe et al. , 2008 ; Krueger et al. , 2009 ). When plants experience reduced...”
- “...co-expression network ( Fig. 2 ; Table 1 ). Of the five SERAT genes, SERAT1;1 (At5g56760), SERAT2;1 (At1g55920), SERAT2;2 (At3g13110), SERAT3;1 (At2g17640), and SERAT3;2 (At4g35640), each has a different transcriptional response to certain conditions ( Watanabe and Hoefgen, 2017 ). It was shown that SERAT group...”
- Photorespiration: regulation and new insights on the potential role of persulfidation
Aroca, Journal of experimental botany 2023 - “...2 P (3) AT3G13110 Q39218 SAT3 Serine acetyltransferase 3, mitochondrial P (3) , S (5,7) AT5G56760 Q42538 SAT5 Serine acetyltransferase 5 P (3) AT5G04590 Q9LZ66 SIR Assimilatory sulfite reductase (ferredoxin) chloroplastic P (3,4) , S (6,7) AT1G79230 O64530 STR1 Thiosulfate/3-mercaptopyruvate sulfurtransferase 1 mitochondrial P (3,4) ,...”
- A molecular switch in sulfur metabolism to reduce arsenic and enrich selenium in rice grain
Sun, Nature communications 2021 - “...were used as the primary antibodies. Microscale thermophoresis analysis Ten C-terminal residues 38 of AtSAT5 (AT5G56760, sequence: FISEWSDYII), required for binding with OAS-TL, was synthesized by GenScript Biotech (Nanjing). Recombinant His-tagged OAS-TL proteins were labeled with RED-tris-NTA 2 nd Generation Dye for 30min. For the binding...”
- Chemical Priming by Isothiocyanates Protects Against Intoxication by Products of the Mustard Oil Bomb
Ferber, Frontiers in plant science 2020 - “...AT5G27380) and all five cysteine forming enzymes of the serine acetyltransferase (SAT) gene family: SERAT1;1 (At5g56760, SAT-c), SERAT2;1 (At1g55920, SAT-p), SERAT2;2 (At3g13110, SAT-m), SERAT3;1 (At2g17640, SAT-106) and SERAT3;2 (At4g35640). Except for SERAT2;1 which was found to be induced by 1.6-fold by SF 4 h after treatment,...”
- Tandem Fluorescent Protein Timers for Noninvasive Relative Protein Lifetime Measurement in Plants
Zhang, Plant physiology 2019 - “...fascinating and extensive plasticity of the plant proteome. MATERIALS AND METHODS SAT5 Construct Full-length SAT5 (AT5G56760) was amplified from Arabidopsis ( Arabidopsis thaliana ) leaf cDNA using the SAT5-tFT_For and SAT5-tFT_Rev primers by PCR and fused with the tFT tag that was PCR amplified from pMaM17...”
- “...data from this article can be found in the GenBank data library under accession numbers At5g56760 (SAT5), At3g24800 (PRT1), At5g02310 (PRT6), At1g04250 (IAA17), At5g25890 (IAA28), At3g17600 (IAA31), At3g62980 (TIR1), and At3g26810 (AFB2). Supplemental Data The following supplemental materials are available. Supplemental Figure S1. Nuclear-cytosolic localization of...”
- The Effect of Single and Multiple SERAT Mutants on Serine and Sulfur Metabolism
Watanabe, Frontiers in plant science 2018 - “...in each subcellular compartment. Table 1 SERAT gene family. Gene name AGI code Localization SERAT1;1 At5g56760 Cytosol SERAT2;1 At1g55920 Plastids SERAT2;2 At3g13110 Mitochondria SERAT3;1 At2g17640 Cytosol SERAT3;2 At4g45640 Cytosol Despite of the fact that OAS and cysteine are synthesized in multiple subcellular compartments, Arabidopsis knockout mutants...”
- Characterization of the serine acetyltransferase gene family of Vitis vinifera uncovers differences in regulation of OAS synthesis in woody plants
Tavares, Frontiers in plant science 2015 - “...cysteine synthesis and contributes in leaves approximately 80% of total SERAT activity, while cytosolic AtSERAT1;1 (At5g56760) and plastidic AtSERAT2;1 (At1g55920) amount to 15 and 5% of the remaining total SERAT activity, respectively (Haas et al., 2008 ; Watanabe et al., 2008 ). The insignificant contribution to...”
- Affinity Purification of O-Acetylserine(thiol)lyase from Chlorella sorokiniana by Recombinant Proteins from Arabidopsis thaliana
Salbitani, Metabolites 2014 - “...S-starved cultures were used for the purification of OASTL proteins by interaction with recombinant AtSAT5 (At5g56760). The crude extract preparation was already described in the previous paragraph (3.2). AtSAT5 protein from Arabidopsis thaliana was over-expressed in Escherichia coli HMS174 (DE3) and immobilized on a nickel-loaded Hi-trap...”
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SAT1-a / Q39218 serine acetyltransferase (EC 2.3.1.30) from Arabidopsis thaliana (see 4 papers)
SAT3_ARATH / Q39218 Serine acetyltransferase 3, mitochondrial; AtSAT-3; AtSERAT2;2; SAT-m; EC 2.3.1.30 from Arabidopsis thaliana (Mouse-ear cress) (see 7 papers)
Q39218 serine O-acetyltransferase (EC 2.3.1.30) from Arabidopsis thaliana (see 4 papers)
AT3G13110 ATSERAT2;2 (SERINE ACETYLTRANSFERASE 2;2); serine O-acetyltransferase from Arabidopsis thaliana
NP_187918 serine acetyltransferase 2;2 from Arabidopsis thaliana
49% identity, 68% coverage
- catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
subunit: Homomultimer (By similarity). Interacts with OASC. Component of the cysteine synthase complex (CSC) composed of two OAS-TL dimers and one SAT hexamer. - Stress responses of plants through transcriptome plasticity by mRNA alternative polyadenylation
Zhou, Molecular horticulture 2023 - “...Arabidopsis , a novel iso-seq based transcriptome study found that cold induced gene AT1G11280 and AT3G13110 switched to use proximal poly(A) sites while gene AT5G5320 switched to use a distal poly(A) site, and they all increased expression level and had a dramatically expression peak in thenight...”
- “...HSP21, HSP70, HSP70-3, HSP40, HSP101, HSP17.6II, HSP17.6C, AT1G59860, AT4G12400, AT3G09350, AT4G36040 Cold / proximal AT1G11280, AT3G13110 (Zhang et al. 2022 ) distal AT5G53420 Table 2 Dynamic features of plant APA genes related to biotic stress responses Biotic Stress Species Stress resistance related Phenotype changing related Reference...”
- New insights into the regulation of plant metabolism by O-acetylserine: sulfate and beyond
Apodiakou, Journal of experimental botany 2023 - “...). In Arabidopsis five genes encoding SERAT proteins can be found: SERAT2;1 (AT1G55920) and SERAT2;2 (AT3G13110), which are localized in plastids and mitochondria, respectively, and SERAT1;1 (AT5G56760), SERAT3;1 (AT2G17640), and SERAT3;2 (AT4G35640), which are localized and expressed in the cytosol ( Watanabe et al. , 2008...”
- “...2 ; Table 1 ). Of the five SERAT genes, SERAT1;1 (At5g56760), SERAT2;1 (At1g55920), SERAT2;2 (At3g13110), SERAT3;1 (At2g17640), and SERAT3;2 (At4g35640), each has a different transcriptional response to certain conditions ( Watanabe and Hoefgen, 2017 ). It was shown that SERAT group III genes are highly...”
- Photorespiration: regulation and new insights on the potential role of persulfidation
Aroca, Journal of experimental botany 2023 - “...synthase mitochondrial P (3,4) , S (5,7) AT2G17640 Q8S895 SAT2 Serine acetyltransferase 2 P (3) AT3G13110 Q39218 SAT3 Serine acetyltransferase 3, mitochondrial P (3) , S (5,7) AT5G56760 Q42538 SAT5 Serine acetyltransferase 5 P (3) AT5G04590 Q9LZ66 SIR Assimilatory sulfite reductase (ferredoxin) chloroplastic P (3,4) ,...”
- A high-resolution single-molecule sequencing-based Arabidopsis transcriptome using novel methods of Iso-seq analysis
Zhang, Genome biology 2022 - “....11 transcript does not change significantly in response to light-dark or cold (Fig. 6 A). AT3G13110 is a single-exon gene. The .1 and .2 isoforms have the same poly(A) sites but the TSS of .2 is 272bp upstream of .1. The .2 transcript codes for a...”
- Glutathione degradation activity of γ-glutamyl peptidase 1 manifests its dual roles in primary and secondary sulfur metabolism in Arabidopsis
Ito, The Plant journal : for cell and molecular biology 2022 - “..., AT4G39940), ATP sulfurylase 3 ( APS3 , AT4G14680), and serine acetyltransferase2;2 ( SERAT2;2 , AT3G13110). Our metabolome data also support the hypothesis of the dual roles of GGPs (Figure 4 ). As discussed in the previous section, many GSLs and ITCs showed significant decreases or...”
- Chemical Priming by Isothiocyanates Protects Against Intoxication by Products of the Mustard Oil Bomb
Ferber, Frontiers in plant science 2020 - “...enzymes of the serine acetyltransferase (SAT) gene family: SERAT1;1 (At5g56760, SAT-c), SERAT2;1 (At1g55920, SAT-p), SERAT2;2 (At3g13110, SAT-m), SERAT3;1 (At2g17640, SAT-106) and SERAT3;2 (At4g35640). Except for SERAT2;1 which was found to be induced by 1.6-fold by SF 4 h after treatment, none of these genes appeared to...”
- The Effect of Single and Multiple SERAT Mutants on Serine and Sulfur Metabolism
Watanabe, Frontiers in plant science 2018 - “...SERAT gene family. Gene name AGI code Localization SERAT1;1 At5g56760 Cytosol SERAT2;1 At1g55920 Plastids SERAT2;2 At3g13110 Mitochondria SERAT3;1 At2g17640 Cytosol SERAT3;2 At4g45640 Cytosol Despite of the fact that OAS and cysteine are synthesized in multiple subcellular compartments, Arabidopsis knockout mutants lacking one or retaining only one...”
- Genome-wide analysis of overlapping genes regulated by iron deficiency and phosphate starvation reveals new interactions in Arabidopsis roots
Li, BMC research notes 2015 - “...protein 1.55 0.27 2.13 0.21 At1G05300 ZIP5, zinc transporter 5 precursor 0.49 0.05 2.13 0.11 At3G13110 ATSERAT2;2, SAT-1, SAT-A, SAT-M, SAT3, SERAT2;2, serine acetyltransferase 2;2 0.83 0.08 2.12 0.22 At2G22290 ATRAB-H1D, ATRAB6, ATRABH1D, RAB-H1D, RABH1d, RAB GTPase homolog H1D 1.91 0.66 2.10 0.59 At4G20160 RING/U-box superfamily...”
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- Mitochondrial serine acetyltransferase functions as a pacemaker of cysteine synthesis in plant cells.
Haas, Plant physiology 2008 - GeneRIF: Mitochondrial serine acetyltransferase regulates the production of O-acetylserine in the mitochondria, which affects cysteine synthesis.
- Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis.
Watanabe, The Plant cell 2008 - GeneRIF: Mitochondrial SERAT2;2 plays a predominant role in cellular OAS formation.
- In silico assessment of gene function involved in cysteine biosynthesis in Arabidopsis: expression analysis of multiple isoforms of serine acetyltransferase.
Noji, Amino acids 2006 (PubMed)- GeneRIF: From the results of sensitivity to feedback regulation, subcellular localization and expression of mRNA analyses, each serine acetyltransferase (SATase) isoform seems to have its specific role for cysteine biosynthesis.
- Photorespiration: regulation and new insights on the potential role of persulfidation
Aroca, Journal of experimental botany 2023 - “...mitochondrial P (3,4) , S (5,7) AT2G17640 Q8S895 SAT2 Serine acetyltransferase 2 P (3) AT3G13110 Q39218 SAT3 Serine acetyltransferase 3, mitochondrial P (3) , S (5,7) AT5G56760 Q42538 SAT5 Serine acetyltransferase 5 P (3) AT5G04590 Q9LZ66 SIR Assimilatory sulfite reductase (ferredoxin) chloroplastic P (3,4) , S...”
LOC110773697 serine acetyltransferase 1, chloroplastic-like from Prunus avium
A0A6P5U464 serine O-acetyltransferase from Prunus avium
53% identity, 65% coverage
- Application of proteomics to determine the mechanism of ozone on sweet cherries (Prunus avium L.) by time-series analysis
Zhao, Frontiers in plant science 2023 - “...synthase alpha chain -1.22 LOC110760963 A0A6P5SZC9 LOW QUALITY PROTEIN: pyruvate kinase isozyme A, chloroplastic-like -0.78 LOC110773697 A0A6P5U464 serine acetyltransferase 1, chloroplastic-like -0.65 LOC110751150 A0A6P5S1I7 serine acetyltransferase 5 0.89 LOC110768684 A0A6P5TMK4 uncharacterized protein LOC110768684 -0.67 LOC110753532 A0A6P5S8M0 fructose-bisphosphate aldolase 1, cytoplasmic -0.69 LOC110762488 A0A6P5SVE9 cytosolic enolase 3...”
- “...A, chloroplastic-like -0.78 LOC110749634 A0A6P5RR27 probable 6-phosphogluconolactonase 4, chloroplastic -0.70 LOC110773146 A0A6P5U2L1 S-formylglutathione hydrolase -0.61 LOC110773697 A0A6P5U464 serine acetyltransferase 1, chloroplastic-like -0.65 LOC110751150 A0A6P5S1I7 serine acetyltransferase 5 0.89 LOC110768684 A0A6P5TMK4 uncharacterized protein LOC110768684 -0.67 LOC110753532 A0A6P5S8M0 fructose-bisphosphate aldolase 1, cytoplasmic -0.69 LOC110762488 A0A6P5SVE9 cytosolic enolase 3...”
- Application of proteomics to determine the mechanism of ozone on sweet cherries (Prunus avium L.) by time-series analysis
Zhao, Frontiers in plant science 2023 - “...alpha chain -1.22 LOC110760963 A0A6P5SZC9 LOW QUALITY PROTEIN: pyruvate kinase isozyme A, chloroplastic-like -0.78 LOC110773697 A0A6P5U464 serine acetyltransferase 1, chloroplastic-like -0.65 LOC110751150 A0A6P5S1I7 serine acetyltransferase 5 0.89 LOC110768684 A0A6P5TMK4 uncharacterized protein LOC110768684 -0.67 LOC110753532 A0A6P5S8M0 fructose-bisphosphate aldolase 1, cytoplasmic -0.69 LOC110762488 A0A6P5SVE9 cytosolic enolase 3 -0.70...”
- “...chloroplastic-like -0.78 LOC110749634 A0A6P5RR27 probable 6-phosphogluconolactonase 4, chloroplastic -0.70 LOC110773146 A0A6P5U2L1 S-formylglutathione hydrolase -0.61 LOC110773697 A0A6P5U464 serine acetyltransferase 1, chloroplastic-like -0.65 LOC110751150 A0A6P5S1I7 serine acetyltransferase 5 0.89 LOC110768684 A0A6P5TMK4 uncharacterized protein LOC110768684 -0.67 LOC110753532 A0A6P5S8M0 fructose-bisphosphate aldolase 1, cytoplasmic -0.69 LOC110762488 A0A6P5SVE9 cytosolic enolase 3 -0.70...”
Q6IWG0 serine O-acetyltransferase (EC 2.3.1.30) from Noccaea goesingensis (see paper)
49% identity, 68% coverage
B9S9Q4 serine O-acetyltransferase from Ricinus communis
53% identity, 66% coverage
LOC107808059 serine acetyltransferase 1, chloroplastic-like from Nicotiana tabacum
49% identity, 75% coverage
- Alternative Oxidase Inhibition Impairs Tobacco Root Development and Root Hair Formation
Liu, Frontiers in plant science 2021 - “...noted that several DEGs (|log 2 FC 1|) were commonly enriched in these pathways, i.e., LOC107808059 and LOC107804363, which encode serine acetyltransferase, were enriched in the KEGG pathways of Carbon metabolism, Biosynthesis of amino acids, and Cysteine and methionine metabolism, and showed upregulation on SHAM treatment...”
SAT4_ARATH / Q8W2B8 Serine acetyltransferase 4; AtSAT-4; AtSERAT3;2; EC 2.3.1.30 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q8W2B8 serine O-acetyltransferase (EC 2.3.1.30) from Arabidopsis thaliana (see paper)
AT4G35640 ATSERAT3;2 (Serine acetyltransferase 3;2); acetyltransferase/ serine O-acetyltransferase from Arabidopsis thaliana
NP_195289 serine acetyltransferase 3;2 from Arabidopsis thaliana
50% identity, 71% coverage
- catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
subunit: Homomultimer. - Mapping the Influence of Light Intensity on the Transgenerational Genetic Architecture of Arabidopsis thaliana
Mei, Current issues in molecular biology 2024 - “...stress response. AT4G35890 , AT4G36590, and AT4G36650 are related to transcriptional regulation, and AT4G35650 , AT4G35640, and AT4G36090 are related to anabolism. The genes duplicated under low light also included AT1G16440 and AT2G27520 , which are related to developmental regulation; AT1G16670 , AT2G27580, and AT2G28060, which...”
- “...4 T/T synonymous Plant-specific TFIIB-related protein Anabolism AT4G35650 16910021 4 T/T 3_prime_UTR Isocitrate dehydrogenase III AT4G35640 16911160 4 A/A upstream_gene Serine acetyltransferase 3;2 AT4G36090 17079871 4 T/T synonymous Oxidoreductase, 2OG-Fe(II) oxygenase family protein AT4G36250 17151190 4 A/A synonymous Aldehyde dehydrogenase 3F1 AT4G36360 17183722 4 T/T upstream_gene...”
- Identification of QTNs, QTN-by-environment interactions, and their candidate genes for salt tolerance related traits in soybean
Chen, BMC plant biology 2024 - “...Glyma13g33590 AT1G53580 GLY3 glyoxalase II 3 FWR Gm16:26,218,707 16 26,218,707 26.1218 2.9313 1.42E-23 SIG Glyma16g22630 AT4G35640 SERAT3;2 serine acetyltransferase 3;2 DWR the dry weights of roots, FWR the fresh weights of roots, LR the length of main root, LH the length of hypocotyls, Chr chromosome, Pos...”
- New insights into the regulation of plant metabolism by O-acetylserine: sulfate and beyond
Apodiakou, Journal of experimental botany 2023 - “...which are localized in plastids and mitochondria, respectively, and SERAT1;1 (AT5G56760), SERAT3;1 (AT2G17640), and SERAT3;2 (AT4G35640), which are localized and expressed in the cytosol ( Watanabe et al. , 2008 ; Krueger et al. , 2009 ). When plants experience reduced sulfate levels, OAS accumulates concomitant...”
- “..., 2008 ) ChaC-like/GGCT2;1 AT5G26220 7 Sulfur metabolism, glutathione process Paulose et al. (2013) SERAT3;2 AT4G35640 4 Sulfur metabolism, cysteine Kawashima et al. (2005) SDI1 AT5G48850 16 Sulfur metabolism, carbon metabolism, transcription Maruyama-Nakashita et al. (2005) SHM7 AT1G36370 12 Sulfur metabolism, carbon metabolism, methionine process Huang...”
- Impact of the SnRK1 protein kinase on sucrose homeostasis and the transcriptome during the diel cycle
Peixoto, Plant physiology 2021 - “...GGCT2;1 GAMMA-GLUTAMYL CYCLOTRANSFERASE 2;1 UP (leaves) 2.21 AT3G08860 PYD4 PYRIMIDINE 4 UP (leaves) 2.69 3.21 AT4G35640 SERAT3;2 SERINE ACETYLTRANSFERASE 3;2 UP (leaves) 1.04 AT4G31330 Putative transmembrne protein (DUF599) UP (leaves) 1.21 AT1G12030 Putative phosphoenolpyruvate carboxylase (DUF506) UP (leaves) 4.78 3.88 AT5G37260 RVE2 REVEILLE 2 DOWN (roots)...”
- Chemical Priming by Isothiocyanates Protects Against Intoxication by Products of the Mustard Oil Bomb
Ferber, Frontiers in plant science 2020 - “...family: SERAT1;1 (At5g56760, SAT-c), SERAT2;1 (At1g55920, SAT-p), SERAT2;2 (At3g13110, SAT-m), SERAT3;1 (At2g17640, SAT-106) and SERAT3;2 (At4g35640). Except for SERAT2;1 which was found to be induced by 1.6-fold by SF 4 h after treatment, none of these genes appeared to be regulated by SF in our microarray...”
- Cytokinin at the Crossroads of Abiotic Stress Signalling Pathways
Pavlů, International journal of molecular sciences 2018 - “...[ 178 ] APR3 AT4G21990 5-adenylylsulfate reductase 3 0/1 [ 178 , 180 ] SAT4 AT4G35640 Serine acetyltransferase 4 [ 181 , 182 ] BGLU28 AT2G44460 Beta-glucosidase 28 [ 179 , 180 ] BGLU30 AT3G60140 Beta-glucosidase 30 [ 180 , 182 ] SDI1 AT5G48850 Protein SULFUR...”
- Analysis of Promoters of Arabidopsis thaliana Divergent Gene Pair SERAT3;2 and IDH-III Shows SERAT3;2 Promoter is Nested Within the IDH-III Promoter
Raipuria, Molecular biotechnology 2017 (PubMed)- “...arranged gene pair SERAT3;2 and IDH-III encoded by At4g35640 and At4g35650 genes, respectively. As per TAIR annotation, these two genes are separated by a...”
- “...the IGR separating the divergent gene pair At4g35640 and At4g35650 coding, respectively, for Serine acetyl transferase (SERAT3;2) and isocitrate dehydrogenase...”
- Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana
Kissen, BMC genomics 2016 - “...Of these five genes, the ones encoding the plastidic SERAT2.1 (At1g55920) and the cytosolic SERAT3.2 (At4g35640) isoforms were induced by ITC, the former being upregulated at an earlier time point and the latter to a higher extent. Consistent with the downregulation of GSL biosynthetic genes discussed...”
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- Analysis of Promoters of Arabidopsis thaliana Divergent Gene Pair SERAT3;2 and IDH-III Shows SERAT3;2 Promoter is Nested Within the IDH-III Promoter.
Raipuria, Molecular biotechnology 2017 (PubMed)- GeneRIF: Study has identified a unique bidirectional promoter capable of driving gene expression in Arabidopsis thaliana, and showed for the first time, that SERAT3;2 promoter is nested in the IDH-III promoter.
- Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis.
Watanabe, The Plant cell 2008 - GeneRIF: SERAT3;2 may play a major role during seed development.
- In silico assessment of gene function involved in cysteine biosynthesis in Arabidopsis: expression analysis of multiple isoforms of serine acetyltransferase.
Noji, Amino acids 2006 (PubMed)- GeneRIF: From the results of sensitivity to feedback regulation, subcellular localization and expression of mRNA analyses, each serine acetyltransferase (SATase) isoform seems to have its specific role for cysteine biosynthesis.
Q6IWF9 serine O-acetyltransferase (EC 2.3.1.30) from Noccaea goesingensis (see paper)
50% identity, 83% coverage
LOC110751150 serine acetyltransferase 5 from Prunus avium
53% identity, 87% coverage
- Application of proteomics to determine the mechanism of ozone on sweet cherries (Prunus avium L.) by time-series analysis
Zhao, Frontiers in plant science 2023 - “...QUALITY PROTEIN: pyruvate kinase isozyme A, chloroplastic-like -0.78 LOC110773697 A0A6P5U464 serine acetyltransferase 1, chloroplastic-like -0.65 LOC110751150 A0A6P5S1I7 serine acetyltransferase 5 0.89 LOC110768684 A0A6P5TMK4 uncharacterized protein LOC110768684 -0.67 LOC110753532 A0A6P5S8M0 fructose-bisphosphate aldolase 1, cytoplasmic -0.69 LOC110762488 A0A6P5SVE9 cytosolic enolase 3 -0.70 LOC110758653 A0A6P5SRD7 3-dehydroquinate synthase, chloroplastic -0.61...”
- “...4, chloroplastic -0.70 LOC110773146 A0A6P5U2L1 S-formylglutathione hydrolase -0.61 LOC110773697 A0A6P5U464 serine acetyltransferase 1, chloroplastic-like -0.65 LOC110751150 A0A6P5S1I7 serine acetyltransferase 5 0.89 LOC110768684 A0A6P5TMK4 uncharacterized protein LOC110768684 -0.67 LOC110753532 A0A6P5S8M0 fructose-bisphosphate aldolase 1, cytoplasmic -0.69 LOC110762488 A0A6P5SVE9 cytosolic enolase 3 -0.70 LOC110752818 A0A6P5S118 dihydrolipoyllysine-residue succinyltransferase component of...”
A0A0C5DES6 serine O-acetyltransferase (EC 2.3.1.30) from Vitis vinifera (see paper)
46% identity, 85% coverage
Rru_A0792 Serine O-acetyltransferase from Rhodospirillum rubrum ATCC 11170
48% identity, 92% coverage
- A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis
Erb, Nature chemical biology 2012 - “...were two putative O -acetyl-homoserine sulfhydrylases (Rru_A0774, Rru_A0784). Notably, four of these five proteins (Rru_A0779, Rru_A0792, and the two sulfhydrylases) were encoded by the same genetic region of fourteen different genes presumably involved in thiol metabolism at the level of cysteine and methionine (thiol cluster, Fig....”
SAT2_ARATH / Q8S895 Serine acetyltransferase 2; AtSAT-2; AtSERAT3;1; EC 2.3.1.30 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
NP_565421 Trimeric LpxA-like enzymes superfamily protein from Arabidopsis thaliana
AT2G17640 ATSERAT3;1; acetyltransferase/ serine O-acetyltransferase from Arabidopsis thaliana
50% identity, 77% coverage
- catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
subunit: Homomultimer. - Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis.
Watanabe, The Plant cell 2008 - GeneRIF: SERAT3;1 may play a major role during seed development.
- In silico assessment of gene function involved in cysteine biosynthesis in Arabidopsis: expression analysis of multiple isoforms of serine acetyltransferase.
Noji, Amino acids 2006 (PubMed)- GeneRIF: From the results of sensitivity to feedback regulation, subcellular localization and expression of mRNA analyses, each serine acetyltransferase (SATase) isoform seems to have its specific role for cysteine biosynthesis.
- Characterization and expression analysis of a serine acetyltransferase gene family involved in a key step of the sulfur assimilation pathway in Arabidopsis.
Kawashima, Plant physiology 2005 - GeneRIF: Serat3;1 and Serat3;2, were characterized with respect to their enzymatic properties, feedback inhibition by L-Cys, and subcellular localization. [Serat3;1]
- Photorespiration: regulation and new insights on the potential role of persulfidation
Aroca, Journal of experimental botany 2023 - “..., N (11) AT3G59760 Q43725 OASC Cysteine synthase mitochondrial P (3,4) , S (5,7) AT2G17640 Q8S895 SAT2 Serine acetyltransferase 2 P (3) AT3G13110 Q39218 SAT3 Serine acetyltransferase 3, mitochondrial P (3) , S (5,7) AT5G56760 Q42538 SAT5 Serine acetyltransferase 5 P (3) AT5G04590 Q9LZ66 SIR Assimilatory...”
- New insights into the regulation of plant metabolism by O-acetylserine: sulfate and beyond
Apodiakou, Journal of experimental botany 2023 - “...and SERAT2;2 (AT3G13110), which are localized in plastids and mitochondria, respectively, and SERAT1;1 (AT5G56760), SERAT3;1 (AT2G17640), and SERAT3;2 (AT4G35640), which are localized and expressed in the cytosol ( Watanabe et al. , 2008 ; Krueger et al. , 2009 ). When plants experience reduced sulfate levels,...”
- “...Table 1 ). Of the five SERAT genes, SERAT1;1 (At5g56760), SERAT2;1 (At1g55920), SERAT2;2 (At3g13110), SERAT3;1 (At2g17640), and SERAT3;2 (At4g35640), each has a different transcriptional response to certain conditions ( Watanabe and Hoefgen, 2017 ). It was shown that SERAT group III genes are highly induced under...”
- Photorespiration: regulation and new insights on the potential role of persulfidation
Aroca, Journal of experimental botany 2023 - “...(5,6,7) , N (11) AT3G59760 Q43725 OASC Cysteine synthase mitochondrial P (3,4) , S (5,7) AT2G17640 Q8S895 SAT2 Serine acetyltransferase 2 P (3) AT3G13110 Q39218 SAT3 Serine acetyltransferase 3, mitochondrial P (3) , S (5,7) AT5G56760 Q42538 SAT5 Serine acetyltransferase 5 P (3) AT5G04590 Q9LZ66 SIR...”
- Analysis of Alternative Splicing During the Combinatorial Response to Simultaneous Copper and Iron Deficiency in Arabidopsis Reveals Differential Events in Genes Involved in Amino Acid Metabolism
Mancini, Frontiers in plant science 2022 - “...AT2G13360 ALANINE:GLYOXYLATE AMINOTRANSFERASE (AGT) Serine-glycine-cysteine group.cysteine.OASTL AT3G22460 O-ACETYLSERINE (THIOL) LYASE ISOFORM A2 (OASA2) Serine-glycine-cysteine group.cysteine.SAT AT2G17640 SERINE O-ACETYLTRANSFERASE (ATSERAT3;1) Aromatic aa.tryptophan.phosphoribosyanthranilate isom AT5G05590 PHOSPHORIBOSYLANTHRANILATE ISOMERASE 2 (PAI2) Aromatic aa.tryptophan.phosphoribosyanthranilate isom AT1G07780 PHOSPHORIBOSYLANTHRANILATE ISOMERASE 1 (PAI1) Aromatic aa.tryptophan.indole-3-glycerol phosphate synt AT5G48220 Indole-3-glycerol phosphate synthase, putative (InGPS) Aromatic...”
- Chemical Priming by Isothiocyanates Protects Against Intoxication by Products of the Mustard Oil Bomb
Ferber, Frontiers in plant science 2020 - “...serine acetyltransferase (SAT) gene family: SERAT1;1 (At5g56760, SAT-c), SERAT2;1 (At1g55920, SAT-p), SERAT2;2 (At3g13110, SAT-m), SERAT3;1 (At2g17640, SAT-106) and SERAT3;2 (At4g35640). Except for SERAT2;1 which was found to be induced by 1.6-fold by SF 4 h after treatment, none of these genes appeared to be regulated by...”
- The Effect of Single and Multiple SERAT Mutants on Serine and Sulfur Metabolism
Watanabe, Frontiers in plant science 2018 - “...Gene name AGI code Localization SERAT1;1 At5g56760 Cytosol SERAT2;1 At1g55920 Plastids SERAT2;2 At3g13110 Mitochondria SERAT3;1 At2g17640 Cytosol SERAT3;2 At4g45640 Cytosol Despite of the fact that OAS and cysteine are synthesized in multiple subcellular compartments, Arabidopsis knockout mutants lacking one or retaining only one compartment specific SERAT...”
- Agronomic and Seed Quality Traits Dissected by Genome-Wide Association Mapping in Brassica napus
Körber, Frontiers in plant science 2016 - “...17808711 FAR7 832303 AT5G22420 78 16964614 16964759 16964687 844025 SUL SUL.A6.s.1 A06 18128799 ATSERAT3;1 816271 AT2G17640 76 17771333 17772947 17772140 356659 APK3 821077 AT3G03900 76 17301192 17301351 17301272 827528 APK4 836888 AT5G67520 76 17300756 17301552 17301154 827645 SUL.A9.s.1 A09 3084578 APK4 836888 AT5G67520 80 3615223 3615740...”
- “...80 3615223 3615740 3615482 1084222 APK3 821077 AT3G03900 78 3615562 3615705 3615634 1084374 ATSERAT3;1 816271 AT2G17640 75 4588363 4588772 4588568 2057308 For abbreviations of the traits see Table 1 and for the full list of candidate genes see Table S2 . a Chr. is the chromosome...”
- Identification of miRNAs associated with dark-induced senescence in Arabidopsis
Huo, BMC plant biology 2015 - “...all 44 miRNAs were predicted, and some of them, such as NAC1 , At3g28690 , At2g17640 and At2g45160 , were found in the Leaf Senescence Database (LSD). GO and KEGG analysis of 137 miRNAs showed that the predicted target genes were significantly enriched in transcription regulation,...”
- “...(Fig. 6 ). Bioinformatics predictive analysis identified NAC1 , At1g73440 (calmodulin-related protein), At2g03220 (galactoside 2--L-fucosyltransferase), At2g17640 (serine acetyltransferase), At2g26950 (MYB domain protein), At2g45160 (protein lost meristems 1), At28690 (putative protein kinase), At5g54810 (tryptophan synthase beta chain) as target genes of miR164a, miR5020c, miR158b, miR156j, miR159a, miR171a,...”
- Characterization of the serine acetyltransferase gene family of Vitis vinifera uncovers differences in regulation of OAS synthesis in woody plants
Tavares, Frontiers in plant science 2015 - “...minor SERAT isoforms in Arabidopsis, which is in full agreement with low transcription of AtSERAT3;1 (At2g17640) and AtSERAT3;2 (At4g35640) and poor enzymatic activities of recombinant AtSERAT3;1 and AtSERAT3;2 proteins, when compared to the major SERAT belonging to group 1 and 2 (Kawashima et al., 2005 )....”
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XP_003551487 serine acetyltransferase 1, chloroplastic from Glycine max
54% identity, 64% coverage
PA14_54880 putative serine acetyltransferase from Pseudomonas aeruginosa UCBPP-PA14
49% identity, 83% coverage
B9FHA5 serine O-acetyltransferase from Oryza sativa subsp. japonica
52% identity, 79% coverage
- Combining Proteomics and Metabolomics to Analyze the Effects of Spaceflight on Rice Progeny
Zeng, Frontiers in plant science 2022 - “...type-2 1.26 4.0609E-02 Q0INQ6 Serine hydroxymethyl transferase 0.78 1.4128E-02 B9FFK4 Anthranilate synthase component 0.78 4.0609E-02 B9FHA5 Serine O -acetyltransferase 0.77 4.0609E-02 Q84PB1 Phosphoribosyl anthranilate transferase 0.77 9.2369E-05 Q6K6Q1 Phenylalanine ammonia-lyase 1.35 5.9680E-03 Q0JFF8 Hydroxy pyruvate reductase HPR3 1.27 4.8507E-03 B8AGS8 Acetylornithine deacetylase 1.27 3.2391E-03 A0A0P0W7B4 Amidase...”
SAT_SCHPO / Q9US33 Putative serine acetyltransferase; EC 2.3.1.30 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
SPAC1039.08 serine acetyltransferase (predicted) from Schizosaccharomyces pombe
42% identity, 87% coverage
- catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
- Genome wide transcription profiling of the effects of overexpression of Spc1 and its kinase dead mutant in Schizosaccharomyces pombe
Paul, Genomics data 2015 - “...Mam3 Up urg2 SPAC1002.17c.S1 Uracil phosphoribosyltransferase (predicted) Down urg1 SPAC1002.19.S1 GTP cyclohydrolase II (predicted) Down SPAC1039.08 SPAC1039.08.S1 Serine acetyltransferase (predicted) Down SPAC13G7.12c SPAC13G7.12c.S1 Choline kinase (predicted) Down meu1///meu2 SPAC1556.06b.S1 Sequence orphan///sequence orphan (predicted to be involved in meiosis) Down SPAC19A8.14 SPAC19A8.14.S1 Aminoacyl-tRNA hydrolase (predicted) Down hem14...”
- Cellular economy in fission yeast cells continuously cultured with limited nitrogen resources
Chikashige, Scientific reports 2015 - “...Per1 1.77 1.82 2.14 1.86 uga1 induced 4-aminobutyrate aminotransferase (GABA transaminase) 1.54 1.82 2.57 1.82 SPAC1039.08 induced serine acetyltransferase (predicted) 1.46 1.53 2.81 1.82 SPAC323.07c induced MatE family transporter (predicted) 1.01 1.08 2.15 1.63 SPCC1223.09 induced uricase (predicted) 1.25 1.63 2.54 1.60 SPBPB2B2.06c induced phosphoprotein phosphatase...”
- The global transcriptional response of fission yeast to hydrogen sulfide
Jia, PloS one 2011 - “...up acetamidase (predicted) SPAC869.01 2.29 up amidase (predicted) SPBPB2B2.05 2.92 up GMP synthase [glutamine-hydrolyzing] (predicted) SPAC1039.08 2.25 up serine acetyltransferase (predicted) SPAC1002.17c 3.46 up uracil phosphoribosyltransferase (predicted) SPAC4F10.17 2.08 up conserved fungal protein SPCC584.16c 3.23 up sequence orphan SPAC6C3.03c 2.58 up sequence orphan SPBPB7E8.02 2.64 up...”
- Interaction of Epe1 with the heterochromatin assembly pathway in Schizosaccharomyces pombe
Isaac, Genetics 2007 - “...specific for isp5, SPAC922.03, SPAC1039.06, SPAC1039.07, SPAC1039.08, and a moderately expressed gene, SPAPB1A10.11c, as a control. SPAPB1A10.11c encodes...”
- Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study
Rustici, Genome biology 2007 - “...response to high copper ( ptr2 , SPBC13A2.04c, SPAP7G5.06, SPAC5H10.01, SPCC132.04c, SPCC1223.09, SPAC11D3.18c, SPAC11D3.15, and SPAC1039.08). Most of these genes are involved in amino acid metabolism. S. cerevisiae cannot compensate for the loss of Ace1p with a general stress response Wild-type S. cerevisiae is protected from...”
- A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast
Hirashima, Nucleic acids research 2006 - “...SPAC1039.01 atgtcgtctattatggaaaa ttaacagatggctgtccaat SPAC1039.02 atgaagttatcatctttacc taaatttgaacgttagaagc SPAC1039.04 atggagaaatctatatcatc aaaactgctgactctgccat SPAC1039.05 atgaccaaggcgaaaaaaag gaaattatcggaaattatgg SPAC1039.07 atgagttctcatgtaagtaa gctggaccatagccgcgtcg SPAC1039.08 atgactgtgcagacagcgga gcgtggtcaagaaacaagcc SPAC922.03 atgggtttagaacagtttaa tagggatgttccgagcaacc SPAC922.04 atgaagtttttctgggtctc aggtgcaaatttattataat SPAC922.06 atgactgttgaaggacgagt cctaaaggcccatatcgaac SPAC922.07 atgtcagaagatttatttgt ggataattccatgggactat SPAC869.09 atgcctgatcctgcacatat caaggtaacgagcataacta pcm2 atgttctggagttttaacct gaggcacccacatgaatggc SPAC869.06 atgcttcagtattccaagaa acaaacttctttgttctttc SPAC869.05 atgcgcgcttggggttgggt aaaaagcaatatatagcaac SPAC869.03 atgacagaaacagttcttaa gcaacaacatgcattccagt SPAC869.02...”
SY28_RS05065 serine O-acetyltransferase from Meiothermus taiwanensis
49% identity, 64% coverage
tlr0851 serine acetyltransferase from Thermosynechococcus elongatus BP-1
43% identity, 62% coverage
- Indirect Interspecies Regulation: Transcriptional and Physiological Responses of a Cyanobacterium to Heterotrophic Partnership
Bernstein, mSystems 2017 - “...) and metabolism of the amino acids cysteine and methionine ( cysE and metE ; tlr0851 and tlr1190 ; coaX and panC-cmk ; tll1149 and tll2450 ) were light responsive with I i , but a smaller number of these genes exhibited such changes in expression...”
- “...cysteine synthases ( cysK ; tlr0504 and SY28_RS03805 ) and serine O-acetyltransferases ( cysE ; tlr0851 and SY28_RS05065 ), which cogrouped into clusters D and A, respectively. Oxidative stress responses. The relative abundance of T. elongatus transcripts encoding enzymes involved with ROS detoxification generally increased with...”
Cthe_2066 serine O-acetyltransferase from Clostridium thermocellum ATCC 27405
43% identity, 64% coverage
LBDG_53060 serine O-acetyltransferase from Leptolyngbya boryana dg5
45% identity, 62% coverage
A6A40_02220 serine O-acetyltransferase from Azospirillum humicireducens
43% identity, 66% coverage
- Complete genome sequence of the nitrogen-fixing bacterium Azospirillum humicireducens type strain SgZ-5T
Yu, Standards in genomic sciences 2018 - “...Nitrogenase molybdenum-iron protein A6A40_09070 333 nifZ Nitrogenase P-cluster assembly A6A40_09075 306 nifZ Nitrogenase P-cluster assembly A6A40_02220 852 cysE Serine acetyltransferase A6A40_02925 909 draG ADP-ribosyl-[dinitrogen reductase] hydrolase A6A40_02920 891 draT ADP-ribosyl-[dinitrogenase reductase] transferase A6A40_07245 2847 glnD [Protein-PII] uridylyltransferase A6A40_07685 339 glnB Nitrogen regulatory protein P-II A6A40_05220 1200...”
- “...fixation gene cluster of 176.7kb. Besides, an operon containing fixABCX genes (A6A40_02185, A6A40_02190, A6A40_02195 and A6A40_02220) responsible for electron transfer to nitrogenase is located upstream of this gene cluster. Nevertheless, the fixABCX operon is generally regulated by a transcriptional activator NifA protein for all nitrogen-fixing bacteria...”
PA3816 O-acetylserine synthase from Pseudomonas aeruginosa PAO1
45% identity, 65% coverage
CYSE_MYCTU / P95231 Serine acetyltransferase; SAT; EC 2.3.1.30 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
P95231 serine O-acetyltransferase (EC 2.3.1.30) from Mycobacterium tuberculosis (see 2 papers)
NP_216851 serine acetyltransferase from Mycobacterium tuberculosis H37Rv
Rv2335 PROBABLE SERINE ACETYLTRANSFERASE CYSE (SAT) from Mycobacterium tuberculosis H37Rv
45% identity, 63% coverage
- function: Catalyzes the acetylation of serine by acetyl-CoA to produce O-acetylserine (OAS).
catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
disruption phenotype: Strains lacking this gene are shown to be attenuated in a mouse tuberculosis model. - Polycysteine-encoding leaderless short ORFs function as cysteine-responsive attenuators of operonic gene expression in mycobacteria
Canestrari, Molecular microbiology 2020 - “...O-acetylserine sulfhydrylase; Interacts with CysE P9WP55 Rv2335 cysE serine acetyltransferase; acetylates serine to make cysteine P95231...”
- Identification and characterization of serine acetyltransferase encoded by the Mycobacterium tuberculosis Rv2335 gene.
Qiu, International journal of molecular medicine 2013 (PubMed)- GeneRIF: The M. tuberculosis Rv2335 gene was cloned and the CysE protein was expressed in E. coli BL21 (DE3).
- The role of Mycobacterium tuberculosis acetyltransferase and protein acetylation modifications in tuberculosis
Huang, Frontiers in cellular and infection microbiology 2023 - “...(2020) Rv2215 DlaT dihydrothioctylamine virulence factor, metabolism and nitrosation stress regulation Shi and Ehrt (2006) Rv2335 CysE serine regulate the growth rate of mycobacterium Qiu etal. (2014) Rv2416c Rv2669 Rv2704 Eis Rv2669 Rv2704 aminoglycoside antibiotics unknown unknown regulate host protein acetylation modification and immune response, acetylate...”
- Deciphering the biosynthesis of a novel lipid in Mycobacterium tuberculosis expands the known roles of the nitroreductase superfamily
Grigg, The Journal of biological chemistry 2023 - “...context of tyz genes further revealed that they are flanked by cysK1 (Rv2334) and cysE (Rv2335), which are putatively involved in cysteine biosynthesis, and mmpl9 (Rv2339). MmpL9 has been implicated in the impairment of phagosome maturation ( 28 ). Intriguingly, transposon mutants in tyzC and mmpL9...”
- Quantitative proteomics reveals that dormancy-related proteins mediate the attenuation in mycobacterium strains
Wang, Virulence 2021 - “...response groups that were upregulated in BCG, namely, two independent cysteine synthesis pathways [CysK1 (Rv2334)-CysE (Rv2335) and CysO (Rv1335)-CysM (Rv1336)] [ 6264 ], succinate dehydrogenase [Sdh1 (Rv0247c and Rv0248c), Sdh2 (sdhC-sdhA)] [ 65 ], and isocitrate lyase [Icl (Rv0467) and AceAa (Rv1915)][ 66 ]. SigB is...”
- Polycysteine-encoding leaderless short ORFs function as cysteine-responsive attenuators of operonic gene expression in mycobacteria
Canestrari, Molecular microbiology 2020 - “...putative attenuating leaderless polycysteine sORF (this report) Rv2334 cysK1 O-acetylserine sulfhydrylase; Interacts with CysE P9WP55 Rv2335 cysE serine acetyltransferase; acetylates serine to make cysteine P95231...”
- Comprehensive analysis of protein acetyltransferases of human pathogen Mycobacterium tuberculosis
Xie, Bioscience reports 2019 - “...CysE can catalyze the chemical reaction transferring the acetyl from AcCoA (acetyl-CoA) to L-Ser (L-serine). Rv2335 was identified and characterized as an serine acetyltransferase in M. tuberculosis [ 67 ], and M. smegmatis homologous cysE knockout mutant strain showed significant morphological changes and suppressed the bacteria...”
- “.... ( 2013 ) Identification and characterization of serine acetyltransferase encoded by the Mycobacterium tuberculosis Rv2335 gene . Int. J. Mol. Med. 31 , 1229 1233 10.3892/ijmm.2013.1298 23483228 68. Qiu J. et al . ( 2014 ) Functional analysis of serine acetyltransferase from Mycobacterium smegmatis ....”
- Population genomics of Mycobacterium tuberculosis in the Inuit
Lee, Proceedings of the National Academy of Sciences of the United States of America 2015 - “...to be essential in genomic screens were completely (Rv2335) or partially (Rv1939, Rv2885c, and Rv3135) deleted in some isolates of the Mj sublineage (Dataset...”
- Functional analysis of serine acetyltransferase from Mycobacterium smegmatis
Qiu, Journal of basic microbiology 2014 (PubMed)- “...mechanism of the bacteria. Mycobacterium tuberculosis CysE (Rv2335) has been identified as a serine acetyltransferase, and it is orthologous to Mycobacterium...”
- “...and kinetic characteristics of M. tuberculosis CysE (Rv2335) [9]. Bioinformatics analysis demonstrated that the M. smegmatis MSMEG_5947 protein is homologous to...”
- Characterizing the pocketome of Mycobacterium tuberculosis and application in rationalizing polypharmacological target selection
Anand, Scientific reports 2014 - “...0.72 A Conserved hypothetical protein Rv3303c lpdA 0.71 A; D; E NAD(P)H quinone reductase LpdA Rv2335 cysE 0.66 A; B; C; E Probable serine acetyltransferase CysE (sat) Rv2398c cysW 0.63 A; D; E Probable sulfate-transport integral membrane protein ABC transporter CysW Rv2689c Rv2689c 0.61 A; D...”
- “...Drug Name CC bp Inferred targets in Mtb Known pharmacological action Atazanavir (DB01072;DR7) 1 Rv2026c; Rv2335; Rv1220; Rv2935 Used in combination with other antiretroviral agents for the treatment of HIV-1 infection, as well as postexposure prophylaxis of HIV infection in individuals who have had occupational or...”
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BCAL2155 serine acetyltransferase from Burkholderia cenocepacia J2315
48% identity, 62% coverage
CYSE_BACSU / Q06750 Serine acetyltransferase; SAT; EC 2.3.1.30 from Bacillus subtilis (strain 168) (see paper)
48% identity, 59% coverage
- function: Catalyzes the acetylation of serine by acetyl-CoA to produce O-acetylserine (OAS)
catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
Ava_1668 Serine O-acetyltransferase from Anabaena variabilis ATCC 29413
43% identity, 62% coverage
BRADO5389 serine acetyltransferase from Bradyrhizobium sp. ORS278
43% identity, 70% coverage
ABUW_2362 serine O-acetyltransferase from Acinetobacter baumannii
45% identity, 62% coverage
alr1404 serine acetyltransferase from Nostoc sp. PCC 7120
44% identity, 61% coverage
LIMLP_00290 serine O-acetyltransferase from Leptospira interrogans serovar Manilae
Q72W82 serine O-acetyltransferase from Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
Q8F9X5 serine O-acetyltransferase from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
45% identity, 59% coverage
- Leptospira interrogans biofilm transcriptome highlights adaption to starvation and general stress while maintaining virulence
Davignon, NPJ biofilms and microbiomes 2024 - “...Sulfur and cysteine metabolism genes cysN , cysD , cysH (LIMLP_17220 to LIMLP_17230) and cysE (LIMLP_00290) were upregulated, suggesting the synthesis of essential sulfur components for FeS cluster construction. Conversely, the downregulation of an EamA-like transporter (LIMLP_02205), potentially involved in cysteine and its metabolite export, suggests...”
- The transcriptional response of pathogenic Leptospira to peroxide reveals new defenses against infection-related oxidative stress
Zavala-Alvarado, PLoS pathogens 2020 - “...rh38 NZ_CP011932.1 -1.078 2.65e-08 3208332148 LIMLP_17965 LIMLP_17960 LIMLP_17970 rh81 NZ_CP011931.1 -1.246 3.21e-11 6734967433 NA LIMLP_00285 LIMLP_00290 rh278 NZ_CP011932.1 -1.188 8.73e-13 202039202120 NA LIMLP_18675 LEPIMA_CII0202 rh331 NZ_CP011931.1 -1.137 9.15e-08 245678245742 NA LIMLP_01140 LEPIMA_CI0236 rh411 NZ_CP011931.1 -1.854 1.53e-65 310470310529 NA LIMLP_01410 ** LIMLP_01415 ** rh418 NZ_CP011931.1 -1.395 9.73e-15...”
- In silico identification of common putative drug targets in Leptospira interrogans
Amineni, Journal of chemical biology 2010 - “...Q8F3U3 argC P59307 34 Q72PE4b Sulfur metabolism 35 Q72W82 36 Q72R95 Lysine degradation 37 Q72SS2a 38 Q72UX3b RNA Polymerase 39 Q72NI8 L-lysine 2,3-aminomutase...”
- In silico identification of common putative drug targets in Leptospira interrogans
Amineni, Journal of chemical biology 2010 - “...acetyltransferase Homoserine O-acetyltransferase cysE metX Q8F9X5 Q8F4I0 Serine acetyltransferase Homoserine O-acetyltransferase Q8EXB5 Q8F6T0b L-lysine pbp1...”
PP0840, PP_0840 serine O-acetyltransferase from Pseudomonas putida KT2440
47% identity, 60% coverage
TepiRe1_2341 serine O-acetyltransferase EpsC from Tepidanaerobacter acetatoxydans Re1
39% identity, 67% coverage
ACIAD2091 serine acetyltransferase from Acinetobacter sp. ADP1
46% identity, 62% coverage
BPSL2248 putative serine acetyltransferase from Burkholderia pseudomallei K96243
46% identity, 62% coverage
Alvin_0863 serine O-acetyltransferase from Allochromatium vinosum DSM 180
47% identity, 61% coverage
- Metabolomic profiling of the purple sulfur bacterium Allochromatium vinosum during growth on different reduced sulfur compounds and malate
Weissgerber, Metabolomics : Official journal of the Metabolomic Society 2014 - “...does not involve formation of phosphoadenosine-5-phosphosulfate (Neumann et al. 2000 ). CysE serine O -acetyltransferase (Alvin_0863), CysM cysteine synthase B (Alvin_2228), GshA glutamate/cysteine ligase (Alvin_800), GshB glutathione synthetase (Alvin_0197), -GluCys -glutamylcysteine, GSH glutathione, XSH glutathione, reduced thioredoxin or glutaredoxin, XSSX oxidized glutathione, thioredoxin or glutaredoxin (see...”
CPE1321 serine O-acetyltransferase from Clostridium perfringens str. 13
48% identity, 61% coverage
SSU1720 serine acetyltransferase from Streptococcus suis P1/7
42% identity, 59% coverage
RS_RS05790 serine O-acetyltransferase from Ralstonia pseudosolanacearum GMI1000
42% identity, 66% coverage
SA0487 hypothetical protein from Staphylococcus aureus subsp. aureus N315
44% identity, 62% coverage
Pnuc_0852 serine O-acetyltransferase from Polynucleobacter sp. QLW-P1DMWA-1
44% identity, 62% coverage
Tmz1t_2208 serine O-acetyltransferase from Thauera sp. MZ1T
45% identity, 64% coverage
CH51_RS02915 serine O-acetyltransferase from Staphylococcus aureus
Q2G2M7 Serine acetyltransferase from Staphylococcus aureus (strain NCTC 8325 / PS 47)
SAUSA300_0514 serine acetyltransferase from Staphylococcus aureus subsp. aureus USA300_FPR3757
46% identity, 59% coverage
DMR38_19595 serine O-acetyltransferase EpsC from Clostridium sp. AWRP
42% identity, 62% coverage
- Metabolic changes of the acetogen Clostridium sp. AWRP through adaptation to acetate challenge
Kwon, Frontiers in microbiology 2022 - “...found to exhibit strong up-regulation of genes involved in serine (DMR38_00400, 00405, and 00415), cysteine (DMR38_19595 and 19600), and methionine biosynthesis (DMR38_03415, 03420, 12485, 03155, 03160, and 09800) as well as the methionine-transporter-encoding gene (DMR38_16080) ( Figure 6B ). The biosynthesis pathways of aromatic amino acids...”
Msp_0450 predicted serine acetyltransferase from Methanosphaera stadtmanae DSM 3091
39% identity, 61% coverage
Cj0763c serine acetyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168
42% identity, 59% coverage
- Cysteine Biosynthesis in Campylobacter jejuni: Substrate Specificity of CysM and the Dualism of Sulfide
Hitchcock, Biomolecules 2022 - “...of C. jejuni Mutant Strains Knock-out deletion mutants of cysM ( cj0912c ), cysE ( cj0763c ) and serA ( cj0891c ) were generated by replacing the majority of the open reading frame with an antibiotic resistance cassette. Mutation plasmids were generated by an isothermal assembly...”
- “...11168 in cysM (cysteine synthase; cj0912c ) and cysE (serine acetyltransferase; deficient in OAS synthesis; cj0763c ). Neither mutant was able to utilise sulfide ( Figure 6 A) or thiosulfate ( Supplementary Figure S2D ) for growth, but both grew normally on L-Cysteine ( Figure 6...”
- Phylogenetic Association and Genetic Factors in Cold Stress Tolerance in Campylobacter jejuni
Hur, Microbiology spectrum 2022 - “...acid transport and metabolism cj0029 ansA Type II asparaginase cj0481 dapA Dihydrodipicolinate synthase family protein cj0763c hisS Histidine-tRNA ligase cj0817 glnH Transporter substrate-binding domain-containing protein cj1726c metA Homoserine O -succinyltransferase cj1727c metB O -Acetylhomoserine aminocarboxypropyltransferase/cysteine synthase Defense mechanisms cj0139 Hypothetical protein cj0690c SAM-dependent DNA methyltransferase cj1549c...”
- Adaptive mechanisms of Campylobacter jejuni to erythromycin treatment
Xia, BMC microbiology 2013 - “...periplasmic binding proteins. Down-regulated genes in this category included argB ( cj0226 ), cysE ( cj0763c ), cj0731 , cj1582c, and cj1583c . Fewer than 3 genes were differentially expressed in other categories (Table 2 ). Different from the inhibitory treatment, the sub-inhibitory treatment resulted in...”
GSU2572 serine acetyltransferase from Geobacter sulfurreducens PCA
40% identity, 67% coverage
K3G22_10455 serine O-acetyltransferase from Shewanella putrefaciens
43% identity, 63% coverage
cysE / CAA68887.1 serine O-acetyltransferase from Staphylococcus xylosus (see paper)
43% identity, 59% coverage
SpyM3_1674 putative serine acetyltransferase from Streptococcus pyogenes MGAS315
43% identity, 59% coverage
- Novel regulatory small RNAs in Streptococcus pyogenes
Tesorero, PloS one 2013 - “...SPyM3_1645 putative response regulator of salavaricin regulon <<< Q, Z SSRC37 SPyM3_1673 hypothetical protein <<< SPyM3_1674 putative serine acetyltransferase <<< Q, Z SSRC38 SPyM3_1725 laminin-binding protein <<< SPyM3_1726 C5A peptidase precursor <<< Q, Z SSRC39 SPyM3_1726 C5A peptidase precursor <<< SPyM3_1727 antiphagocytic M protein, type 3...”
lp_0254 serine O-acetyltransferase from Lactobacillus plantarum WCFS1
F9UT52 Serine acetyltransferase from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
lp_0254 serine O-acetyltransferase EpsC from Lactiplantibacillus plantarum WCFS1
46% identity, 58% coverage
- Molecular Responses of Lactobacilli to Plant Phenolic Compounds: A Comparative Review of the Mechanisms Involved
López, Antioxidants (Basel, Switzerland) 2021 - “...resistance [ 91 ] lp_1253 gshR2 : glutathione reductase p -CA, OLE + Bile resistance lp_0254 cysE : serine O -acetyltransferase HXT, OLE + Bile resistance lp_0255 metC1 : cystathionine beta-lyase HXT, OLE + Bile resistance lp_0256 cysK : cysteine synthase HXT, OLE + Bile resistance...”
- Characterization of transcriptional response of Lactobacillus plantarum under acidic conditions provides insight into bacterial adaptation in fermentative environments
Jung, Scientific reports 2020 - “...1.18E27 3.00E26 Oligopeptide ABC transporter lp_0018 F9US48 COG4166 K02035 cysE 3.18 9.45E87 1.47E84 Serine acetyltransferase lp_0254 F9UT52 COG1045 K00640 cblB 3.69 3.13E95 5.15E93 Cystathionine beta-lyase lp_0255 F9UT53 COG0626 K01760 cbs 3.45 1.28E70 1.41E68 Cystathionine beta-synthase lp_0256 F9UT54 COG0031 K01738 pnuC1 1.64 2.38E218 8.95E216 Nicotinamide mononucleotide transporter...”
- Two homologous Agr-like quorum-sensing systems cooperatively control adherence, cell morphology, and cell viability properties in Lactobacillus plantarum WCFS1
Fujii, Journal of bacteriology 2008 - “...and lamA mutants Open reading frame Downregulated genes lp_0254 lp_0255 lp_2833 lp_3084 lp_3085 lp_3087 lp_3575 lp_3579 lp_3580 lp_3582 a malG amy2 msmK1 ftsH...”
- Thioredoxin reductase is a key factor in the oxidative stress response of Lactobacillus plantarum WCFS1
Serrano, Microbial cell factories 2007 - “...of L. plantarum . Locus FC 2 trx/wt Gene Product Main Functional Class (%) 1 lp_0254 1,5 cysE serine O-acetyltransferase Amino acid biosynthesis (4%) lp_3421 1,6 extracellular protein, gamma-D-glutamate-meso-diaminopimelate muropeptidase (putative) Cell envelope (7%) lp_2716 1,6 ica3 glycosyltransferase (putative) lp_0728 0,6 groEL GroEL chaperonin Cellular processes...”
- An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence
Sturme, Journal of bacteriology 2005 - “...lp_1205 lp_1206 lp_1207 lp_1208 lp_1210 lp_1211 Cluster 4 lp_0254 lp_0255 cps2C galE2 cps2E cps2F cps2G cps2H cps2I cps2J cysE metC1 Description Change (fold)c...”
- Characterization of transcriptional response of Lactobacillus plantarum under acidic conditions provides insight into bacterial adaptation in fermentative environments.
Jung, Scientific reports 2020 - “...3.00E26 Oligopeptide ABC transporter lp_0018 F9US48 COG4166 K02035 cysE 3.18 9.45E87 1.47E84 Serine acetyltransferase lp_0254 F9UT52 COG1045 K00640 cblB 3.69 3.13E95 5.15E93 Cystathionine beta-lyase lp_0255 F9UT53 COG0626 K01760 cbs 3.45 1.28E70 1.41E68 Cystathionine beta-synthase lp_0256 F9UT54 COG0031 K01738 pnuC1 1.64 2.38E218 8.95E216 Nicotinamide mononucleotide transporter lp_0259...”
WP_018370157 serine O-acetyltransferase from Streptococcus marimammalium DSM 18627
42% identity, 58% coverage
- PARGT: a software tool for predicting antimicrobial resistance in bacteria
Chowdhury, Scientific reports 2020 - “...undecaprenol kinase True positive EEK11594 Undecaprenyl-diphosphatase UppP True positive EUJ19660 Hypothetical protein MAQA_05683 False positive WP_018370157 Serine O -acetyltransferase False positive Table 2 Predicted van AMR sequences for Staphylococcus , Streptococcus , and Listeria using the GTDWFE algorithm. NCBI accession number Protein names Note AAQ17160 Vancomycin/teicoplanin...”
- “...(plasmid) True positive AAQ17163 vanZ protein (plasmid) True positive CDC71755 Putative uncharacterized protein False positive WP_018370157 Serine O -acetyltransferase False positive Tables 1 and 2 list the predicted bac and van AMR sequences from our test datasets, respectively. In each table, we provide the NCBI accession...”
CD630_15950, CDIF630erm_01768 serine O-acetyltransferase EpsC from Clostridioides difficile 630
40% identity, 62% coverage
- Human-Gut Phages Harbor Sporulation Genes
Schwartz, mBio 2023 - “...regulator, sporulation initiation inhibitor, chromosome partitioning protein K00390 BSU_10930 yitB NR Phosphoadenosine phosphosulfate reductase K00640 CD630_15950 cysE NR Serine acetyltransferase (SAT) K00820 CD630_1200 glmS NR Glucosamine-fructose-6-phosphate aminotransferase (isomerizing) K00974 BSU_22450 cca NR CCA-adding enzyme K01142 BSU_40880 exoA NR Exodeoxyribonuclease, repair of oxidative DNA damage in spores...”
- Thiol Metabolism and Volatile Metabolome of Clostridioides difficile
Biwer, Frontiers in microbiology 2022 - “...Bogicevic et al., 2016 ). This step may be realized by secondary activity of CysE (CDIF630erm_01768) or another putative acetyltransferase present in C. difficile 630 erm (e.g., CDIF630erm_00789). With a proper leaving group in place, sulfur introduction might follow directly by reaction with cysteine. The cystationine...”
CD1595 serine acetyltransferase from Clostridium difficile 630
41% identity, 59% coverage
- c-di-AMP signaling is required for bile salt resistance, osmotolerance, and long-term host colonization by Clostridioides difficile
Oberkampf, Science signaling 2022 - “...(FAD)dependent oxidoreductase 4 4.73 10 09 CD1796 4Fe-4S binding domain protein 3.57 9.49 10 07 CD1595 ( cysE ) Serine O -acetyltransferase 2.22 4.41 10 04 CD2004 ( effR ) MarR family transcriptional regulator 2.13 2.92 10 03 CD29972999 ABC-type transport system, iron family 2.04 to...”
- Control of Clostridium difficile Physiopathology in Response to Cysteine Availability
Dubois, Infection and immunity 2016 - “...al. are as follows: cysE, serine O-acetyltransferase (CD1595); cysK, OAS-thiol-lyase (CD1594); asrABC, anaerobic sulfite reductase (CD2231 to -2233); ssuCBA1,...”
- “...CD3274 (feoB3) CD2878 (fhuD) CD2875 (fhuC) CD1594 (cysK) CD1595 (cysE) CD1999 (fldX) CD1777 CD1485 CD2499 CD2881 CD2992 CD2991 CD2989 Ferric uptake regulation...”
- Global transcriptional control by glucose and carbon regulator CcpA in Clostridium difficile
Antunes, Nucleic acids research 2012 - “...tdcB (CD2514), threonine dehydratase II; serA (CD0995), putative D-3-phosphoglycerate dehydrogenase; sdaB (CD3222), L-serine dehydratase; cysE (CD1595), serine acetyltransferase; cysK (CD1594), O -acetylserine sulphydrylase; iscS2 (CD1279), cysteine desulphurase; oppBCADF (CD0853CD0857), oligopeptide ABC transporter; CD2177CD2174, cystine ABC transporter; prdF (CD3237), proline racemase; prdE (CD3239), proline reductase; prdA (CD3244),...”
HP1210 serine acetyltransferase (cysE) from Helicobacter pylori 26695
46% identity, 59% coverage
- The pH-responsive regulon of HP0244 (FlgS), the cytoplasmic histidine kinase of Helicobacter pylori
Wen, Journal of bacteriology 2009 - “...protein (kapA) Amino acid biosynthesis HP0380b,c,f HP0330 HP1038 HP1210 1.61 1.63 1.69 1.36 1.51 1.34 1.53 0.93 2.37 2.31 2.67 2.91 Glutamate dehydrogenase...”
- Using macro-arrays to study routes of infection of Helicobacter pylori in three families
Raymond, PloS one 2008 - “...ribosomal protein L7/L12 (rpl7/l12) HP1201 ribosomal protein L1 (rpl1) HP1209 ulcer-associated gene restriction endonuclease (iceA) HP1210 serine acetyltransferase (cysE) HP1220 ABC transporter, ATP-binding protein (yhcG) HP1221 putative undecaprenyl pyrophosphate synthase HP1224 uroporphyrinogen III cosynthase (hemD) HP1236 hypothetical protein HP1250+ hypothetical protein HP1260 NADH-ubiquinone oxidoreductase, NQO7 subunit...”
- Expanded metabolic reconstruction of Helicobacter pylori (iIT341 GSM/GPR): an in silico genome-scale characterization of single- and double-deletion mutants
Thiele, Journal of bacteriology 2005 - “...(CYSS), HP1210 (SERAT) FUMt3...”
- Analysis of iceA genotypes in South African Helicobacter pylori strains and relationship to clinically significant disease
Kidd, Gut 2001 - “...sodium chloride/sodium citrate. 630 Kidd, Peek, Lastovica, et al CysE (HP1210) iceA1 (HP1209) 952 bp 558 bp F1 IF F5 CysE (JHP1133) hpyIM (HP1208) 246 bp R10 IR...”
- “...The complete iceA1 gene, from the upstream cysE (HP1210) to the downstream hypIM (HP1208), was amplified using F1 (5'-GGG TGCGATTTGCGTGGGCGATG) and R10...”
jhp1133 O-SERINE ACETYLTRANSFERASE from Helicobacter pylori J99
Q9ZK14 Serine acetyltransferase from Helicobacter pylori (strain J99 / ATCC 700824)
45% identity, 59% coverage
BB562_01130 serine O-acetyltransferase EpsC from Lactiplantibacillus pentosus
45% identity, 58% coverage
- Transcriptomic Profile and Probiotic Properties of Lactiplantibacillus pentosus Pre-adapted to Edible Oils
Alonso, Frontiers in microbiology 2021 - “...In this manner, additional up-regulated genes included cysteine and methionine metabolism such as serine acetyltransferase (BB562_01130; Supplementary Table 2 and Supplementary Figure 3 ). On the other hand, we detected the absence of up-regulated genes coding histidinol-phosphatase (BB562_04885) involved in histidine metabolic pathway, glucose-1-phosphate adenylyltransferase (BB562_00090)...”
- “...(BB562_12015 and BB562_11990; Supplementary Table 2 and Supplementary Figure 3 ), and encoding serine acetyltransferase (BB562_01130) involved in sulfur metabolism ( Supplementary Table 2 and Supplementary Figure 3 ). Other new metabolic pathways were differentially repressed or induced between TO and TA, such as bacterial secretion...”
TM0666 serine acetyltransferase from Thermotoga maritima MSB8
42% identity, 59% coverage
P23145 Probable serine acetyltransferase from Azotobacter chroococcum mcd 1
42% identity, 65% coverage
Ssal_02089 serine O-acetyltransferase from Streptococcus salivarius 57.I
44% identity, 59% coverage
lmo0238 similar to serine O-acetyltransferase from Listeria monocytogenes EGD-e
Q8YAB2 Serine acetyltransferase from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
LMOf2365_0250 serine O-acetyltransferase from Listeria monocytogenes str. 4b F2365
44% identity, 59% coverage
LMRG_02938 serine O-acetyltransferase EpsC from Listeria monocytogenes 10403S
44% identity, 59% coverage
- Listeria monocytogenes σA Is Sufficient to Survive Gallbladder Bile Exposure
Boonmee, Frontiers in microbiology 2019 - “...protein FtsW 2.56 0.01 LMRG_02481 lmo0052 LMRG_02481-LMRG_02483 Phosphoesterase, DHH family protein 2.07 0.00 LMRG_02665 lmo0241 LMRG_02938 , LMRG_02667-LMRG_02664 TrmH family tRNA/rRNA methyltransferase YacO 2.67 0.00 LMRG_02671 lmo0235 ispD1 LMRG_02672-LMRG_02669 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2.27 0.00 LMRG_01669 and LMRG_00091 Genes Were Differentially Expressed in sigBCHL Compared to Wild...”
ERH_0421 serine O-acetyltransferase from Erysipelothrix rhusiopathiae str. Fujisawa
40% identity, 61% coverage
- Rational Design of Live-Attenuated Vaccines against Genome-Reduced Pathogens
Nishikawa, Microbiology spectrum 2022 - “...asnA , aspartate-ammonia ligase (ERH_1353); glyA , glycine hydroxymethyltransferase (ERH_1608); cysE , serine O -acetyltransferase (ERH_0421); cysK , cysteine synthase A (ERH_0469); glyA , glycine hydroxymethyltransferase (ERH_1608); proA , glutamate-5-semialdehyde dehydrogenase (ERH_0055); proB , glutamate 5-kinase (ERH_0054); proC , pyrroline-5-carboxylate reductase (ERH_0057); arcA , arginine deiminase...”
- The genome of Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, reveals new insights into the evolution of firmicutes and the organism's intracellular adaptations
Ogawa, Journal of bacteriology 2011 - “...O-acetyltransferase (cysE) ...................................................................................................ERH_0421 Cysteine synthase A...”
DIP1891 serine O-acetyltransferase EpsC from Corynebacterium diphtheriae NCTC 13129
40% identity, 61% coverage
A0A0H2UNY1 serine O-acetyltransferase (EC 2.3.1.30) from Streptococcus pneumoniae (see paper)
SPD_0513 serine O-acetyltransferase from Streptococcus pneumoniae D39
43% identity, 59% coverage
STER_RS04390 serine O-acetyltransferase EpsC from Streptococcus thermophilus LMD-9
41% identity, 59% coverage
CW752_RS05270 serine O-acetyltransferase EpsC from Chryseobacterium sp. PMSZPI
38% identity, 62% coverage
cg2834 Serine O-acetyltransferase from Corynebacterium glutamicum ATCC 13032
41% identity, 62% coverage
C7NGP7 Serine acetyltransferase from Kytococcus sedentarius (strain ATCC 14392 / DSM 20547 / JCM 11482 / CCUG 33030 / NBRC 15357 / NCTC 11040 / CCM 314 / 541)
Ksed_26170 serine O-acetyltransferase from Kytococcus sedentarius DSM 20547
44% identity, 58% coverage
STER_0887 Serine acetyltransferase from Streptococcus thermophilus LMD-9
46% identity, 47% coverage
7bw9A / Q401L4 Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
35% identity, 65% coverage
EhSAT serine acetyltransferase 1; EC 2.3.1.30 from Entamoeba dispar (see paper)
30% identity, 76% coverage
J517_0526 serine O-acetyltransferase EpsC from Acinetobacter baumannii 118362
35% identity, 65% coverage
A1S_1407 serine acetyltransferase from Acinetobacter baumannii ATCC 17978
35% identity, 74% coverage
Q401L4 serine O-acetyltransferase (EC 2.3.1.30) from Entamoeba histolytica (see 2 papers)
EHI_153430, XP_656373 serine acetyltransferase 1 from Entamoeba histolytica HM-1:IMSS
34% identity, 54% coverage
- Genetic, metabolomic and transcriptomic analyses of the de novo L-cysteine biosynthetic pathway in the enteric protozoan parasite Entamoeba histolytica
Jeelani, Scientific reports 2017 - “...isoforms by ClustalW multiple sequence alignment score. GenBank accession numbers: SAT1, BAA82868; SAT2, XP_650001; SAT3, XP_656373; CS1, XP_650965; CS2, XP_648291; and CS3, XP_653246. ( C ) Semi-quantitative RT-PCR analysis of SAT1 , SAT2 , SAT3 , CS1 and CS3 gene transcript levels in trophozoites of strain...”
- Global analysis of gene expression in response to L-Cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica
Husain, BMC genomics 2011 - “...MGL1, EHI_144610; MGL2, EHI_142250; NifS; EHI_136380; PGDH, EHI_060860; PSAT, EHI_026360; SAHH, EHI_068250; SAT2, EHI_021570; SAT3, EHI_153430. We have recently shown by metabolomic analysis that the synthesis of OAS and SMC markedly increased upon L-cysteine deprivation in E. histolytica . OAS in bacteria is known to regulate...”
- Isoform-dependent feedback regulation of serine O-acetyltransferase isoenzymes involved in L-cysteine biosynthesis of Entamoeba histolytica.
Hussain, Molecular and biochemical parasitology 2009 (PubMed)- GeneRIF: The three serine O-acetyltransferases showed remarkable differences in their sensitivity to inhibition by L-cysteine.
Q9U8X2 serine O-acetyltransferase (EC 2.3.1.30) from Entamoeba histolytica (see 2 papers)
GI|5689218 serine acetyltransferase; EC 2.3.1.30 from Entamoeba histolytica (see paper)
37% identity, 53% coverage
3p47A / Q9U8X2 Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
37% identity, 59% coverage
SRPH_SYNE7 / Q59967 Serine acetyltransferase, plasmid; SAT; EC 2.3.1.30 from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2) (see paper)
srpH / AAA86726.1 serine acetyltransferase from Synechococcus elongatus PCC 7942 = FACHB-805 (see paper)
35% identity, 56% coverage
- catalytic activity: L-serine + acetyl-CoA = O-acetyl-L-serine + CoA (RHEA:24560)
RAYM_04791 serine O-acetyltransferase from Riemerella anatipestifer RA-YM
36% identity, 57% coverage
Q401L5 serine O-acetyltransferase (EC 2.3.1.30) from Entamoeba histolytica (see 2 papers)
EHI_021570, XP_650001 serine acetyltransferase 1 from Entamoeba histolytica HM-1:IMSS
29% identity, 73% coverage
- Screening and Structural Characterization of Heat Shock Response Elements (HSEs) in <i>Entamoeba histolytica</i> Promoters
Dorantes-Palma, International journal of molecular sciences 2024 - “...like the Rab family GTPase (EHI_003020), 40S ribosomal protein S6 (EHI_000590), and Serine acetyltransferase 1 (EHI_021570). Additionally, 45 genes possessed two HSEs, with Ehpgp5-Ehhsp100 being the most prevalent combination. Representative genes with this combination are the cell division cycle protein 123 homolog (EHI_128440) and syntaxin (EHI_139030)...”
- “...this group are genes such as acetyltransferase (EHI_096770), Acetyltransferase GNAT family (EHI_137930), and Serine O-acetyltransferase (EHI_021570), all involved in acetyl group transfer, and Vacuolar protein sorting 26A (EHI_162540), which plays a role in protein folding ( Supplementary Table S4C ). These findings suggest that a significant...”
- Genetic, metabolomic and transcriptomic analyses of the de novo L-cysteine biosynthetic pathway in the enteric protozoan parasite Entamoeba histolytica
Jeelani, Scientific reports 2017 - “...and CS isoforms by ClustalW multiple sequence alignment score. GenBank accession numbers: SAT1, BAA82868; SAT2, XP_650001; SAT3, XP_656373; CS1, XP_650965; CS2, XP_648291; and CS3, XP_653246. ( C ) Semi-quantitative RT-PCR analysis of SAT1 , SAT2 , SAT3 , CS1 and CS3 gene transcript levels in trophozoites...”
- Phenotypic and transcriptional profiling in Entamoeba histolytica reveal costs to fitness and adaptive responses associated with metronidazole resistance
Penuliar, Frontiers in microbiology 2015 - “...(EHI_006140), and a competence/damage-inducible protein (EHI_090260). In MTZR (), AP-2 complex protein (EHI_014390), serine acetyltransferase (EHI_021570), zinc finger protein (EHI_091050), cysteine protease (EHI_117650), and a cell division control protein (EHI_154270) were specifically modulated. In HM-1 (+), on the other hand, genes for heat shock proteins [EHI_005657...”
- Global analysis of gene expression in response to L-Cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica
Husain, BMC genomics 2011 - “...MAT, 70.m00173; MGL1, EHI_144610; MGL2, EHI_142250; NifS; EHI_136380; PGDH, EHI_060860; PSAT, EHI_026360; SAHH, EHI_068250; SAT2, EHI_021570; SAT3, EHI_153430. We have recently shown by metabolomic analysis that the synthesis of OAS and SMC markedly increased upon L-cysteine deprivation in E. histolytica . OAS in bacteria is known...”
- Isoform-dependent feedback regulation of serine O-acetyltransferase isoenzymes involved in L-cysteine biosynthesis of Entamoeba histolytica.
Hussain, Molecular and biochemical parasitology 2009 (PubMed)- GeneRIF: The three serine O-acetyltransferases showed remarkable differences in their sensitivity to inhibition by L-cysteine.
WP_009993515 serine O-acetyltransferase EpsC from Gardnerella vaginalis
34% identity, 62% coverage
- Mechanisms of S. agalactiae promoting G. vaginalis biofilm formation leading to recurrence of BV
Li, NPJ biofilms and microbiomes 2024 - “...transcriptional regulator WP_004113527 luxS Up NS S-ribosylhomocysteine lyase WP_004115065 NS None LysR substrate-binding domain-containing protein WP_009993515 NS NS serine acetyltransferase WP_009993797 glgP None NS glycogen/starch/alpha-glucan phosphorylase EPITHELIAL ADHESION WP_013399737 Down NS SpaH/EbpB family LPXTG-anchored major pilin WP_009993957 Down NS LPXTG cell wall anchor domain-containing protein WP_004113407...”
SELR_06150 serine acetyltransferase from Selenomonas ruminantium subsp. lactilytica TAM6421
33% identity, 54% coverage
Echvi_0221 serine O-acetyltransferase EpsC from Echinicola vietnamensis DSM 17526
39% identity, 53% coverage
- GapMind: Automated Annotation of Amino Acid Biosynthesis
Price, mSystems 2020 - “...AroA; it appears to be essential Diverged Cysteine: CysE Echinicola vietnamensis KMM 6221, DSM 17526 Echvi_0221 may be a diverged serine acetyltransferase; it appears to be essential Diverged Histidine: HisN Desulfovibrio vulgaris Hildenborough DVU2940 may be a diverged histidinol phosphatase; it appears to be essential (V....”
ACIAD1763 serine acetyltransferase from Acinetobacter sp. ADP1
34% identity, 62% coverage
NE0575 Bacterial transferase hexapeptide repeat from Nitrosomonas europaea ATCC 19718
36% identity, 50% coverage
PP1110, PP_1110 serine O-acetyltransferase, putative from Pseudomonas putida KT2440
35% identity, 57% coverage
- UEG Week 2024 Poster Presentations
, United European gastroenterology journal 2024 - Production of selenium nanoparticles occurs through an interconnected pathway of sulphur metabolism and oxidative stress response in Pseudomonas putida KT2440
Avendaño, Microbial biotechnology 2023 - “...also evidenced variations in the expression of genes related to the metabolism of serine. The PP_1110 gene, underexpressed in the presence of selenite, encodes a serine acetyltransferase, an enzyme that links amino acid and sulphur metabolism. The differential expression of genes related to the biosynthesis of...”
- UEG Week 2023 Poster Presentations
, United European gastroenterology journal 2023 - Comparative Transcriptome Analysis of Pseudomonas putida KT2440 Revealed Its Response Mechanisms to Elevated Levels of Zinc Stress
Peng, Frontiers in microbiology 2018 - “...as revealed by the great upregulation of glutamate synthase (PP_0269). The transcription of hutU and PP_1110 were strongly increased under the high zinc stress. HutU is involved in the second step of the subway that synthesizes L-glutamate from L-histidine, whereas PP_1110 encodes serine acetyltransferase which catalyzes...”
- “...4.93 0.04 4.8 0.12 LysE family transporter PP_0999 arcC 0.27 0.03 0.23 0.09 Carbamate kinase PP_1110 5.81 1.28 Serine acetyltransferase PP_1400 kgtP 0.27 0.01 0.19 0.04 Alpha-ketoglutarate permease PP_2453 ansB 7.07 0.45 3.53 0.33 Glutaminase-asparaginase PP_3593 4.17 0.23 9.00 0.24 Amino acid ABC transporter substrate-binding protein...”
- A two-component regulatory system integrates redox state and population density sensing in Pseudomonas putida
Fernández-Piñar, Journal of bacteriology 2008 - “...mutant Fold induction Locus (gene name) PP0887 PP0888 PP1110 PP1747 PP2379 PP3286 (phaN) PP4250 (ccoN-1) PP4252 (ccoQ-1) PP4253 (ccoP-1) PP5030 (hutI) PP5033...”
- “...(soxB) PP_0324 (soxD) PP_0325 (soxA) PP_1088 (argG) PP_1110 PP_1111 PP_1112 PP_1113 PP_1654 (cysM) PP_5029 (hutG) PP_5030 (hutI) PP_5032 (hutH) PP_5033 (hutU)...”
- Cohabitation of two different lexA regulons in Pseudomonas putida
Abella, Journal of bacteriology 2007 - “...PP_2451 (endA) PP_4019 (topB) PP_5393 (hp) PP_2924 (hp) PP_2923 (hp) PP_1110 (cysE) PP_1111 (hp) PP_1112 (hp) PP_1113 (cysK) c NP NP NP NP NP NP NP NP NP NP...”
- “...and PP_2142; PP_2452 and PP_2451; PP_2924 and PP_2923; PP_1110, PP_1111, PP_1112, and PP_1113; and PP_3116, PP_3117, PP_3118, and PP_3119. c , gene present in...”
- The RpoT regulon of Pseudomonas putida DOT-T1E and its role in stress endurance against solvents
Duque, Journal of bacteriology 2007 - “...PP0812 PP0813 PP0814 PP0816 PP0887 PP0888 PP0951 PP1002 PP1110 PP1111 PP1112 PP1113 PP1149 PP1157 PP1188 PP2246 PP2379 PP2471 PP2648 PP2656 PP2916 PP3005 PP3007...”
- “...PP0810 PP0812 PP0813 PP0814 PP0816 PP0887 PP0888 PP0889 PP1110 PP1111 PP1112 PP1113 PP1157 PP1188 PP1196 PP1747 PP2257 PP2379 PP2554 PP2648 PP2656 PP2737 PP2855...”
PP0228 serine O-acetyltransferase, putative from Pseudomonas putida KT2440
38% identity, 51% coverage
ACIAD1584 serine acetyltransferase from Acinetobacter sp. ADP1
35% identity, 54% coverage
Bache_0784 No description from Bacteroides helcogenes P 36-108, DSM 20613
33% identity, 53% coverage
Ddes_0279 Serine O-acetyltransferase from Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774
32% identity, 52% coverage
XP_003864430 serine acetyltransferase from Leishmania donovani
34% identity, 37% coverage
MXAN_7449 transferase, hexapeptide repeat family from Myxococcus xanthus DK 1622
41% identity, 41% coverage
Dde_3081 Serine O-acetyltransferase from Desulfovibrio desulfuricans G20
31% identity, 46% coverage
- Integration of text mining and biological network analysis: Identification of essential genes in sulfate-reducing bacteria
Saxena, Frontiers in microbiology 2023 - “...observed (shown in Figure 4A ). The enriched genes (dde_0276, dde_0528, dde_1112, dde_1258, dde_2115, dde_2271, dde_3081, dde_3604, cysQ , and sucD ) were not only unconstrained to sulfate metabolism but a strong network to three other pathways- cysteine and methionine metabolism, selenocompound metabolism, and secondary metabolite...”
- “...in the intermediate step of methionine synthesis ( Ekstrom et al., 2003 ). Serine O-acetyltransferase (dde_3081) shares its role with the Seleno-compound metabolism along with the cysteine metabolism, which itself derives from serine ( Turanov et al., 2011 ). With the involvement of more complex enzymatic...”
VC0923 serine acetyltransferase-related protein from Vibrio cholerae O1 biovar eltor str. N16961
32% identity, 48% coverage
Francci3_3842 Serine acetyltransferase-like from Frankia sp. CcI3
31% identity, 56% coverage
- Transcriptomes of Frankia sp. strain CcI3 in growth transitions
Bickhart, BMC microbiology 2011 - “...hypothetical protein Francci3_3302 982 molecular chaperone DnaK Francci3_4352 2036 hypothetical protein Francci3_2442 1117 Serine acetyltransferase-like Francci3_3842 970 globin Francci3_2581 1935 SsgA Francci3_3418 1043 hypothetical protein Francci3_0227 970 LuxR family regulator Francci3_0765 1934 SecE subunit Francci3_0567 1037 hypothetical protein Francci3_1719 965 thioredoxin reductase Francci3_4536 1913 putative Lsr2-like...”
VC0919 serine acetyltransferase-related protein from Vibrio cholerae O1 biovar eltor str. N16961
33% identity, 45% coverage
- Impact of Gene Repression on Biofilm Formation of Vibrio cholerae
Pombo, Frontiers in microbiology 2022 - “...colonization factor AcfD 2 VC0885-886 VC0886 Hypothetical protein 1 VC0900-901 VC0901 Hypothetical protein 1 VC0917-927 VC0919 Serine acetyltransferase-like protein, serine O-acetyltransferase 1 VC0977 Putative thioredoxin 1 VC0998 Tfp pilus assembly protein FimV 2 VC1010 Lactoylglutathione lyase 1 VC1033 Zinc/cadmium/mercury/lead-transporting ATPase 1 VC1092-1095 VC1093 Oligopeptide ABC transporter...”
- The LonA Protease Regulates Biofilm Formation, Motility, Virulence, and the Type VI Secretion System in Vibrio cholerae
Rogers, Journal of bacteriology 2016 - “...VPS and biofilm matrix biosynthesis VC0916 VC0918 VC0919 VC0926 VC0928 VC0930 VC0932 VC0933 VC0935 VC1888 Phosphotyrosine protein phosphatase, vpsU...”
- Structural characterization of the extracellular polysaccharide from Vibrio cholerae O1 El-Tor
Yildiz, PloS one 2014 - “...sugar GulNAcA in the VPS structure. Two genes involved in vps biosynthesis operon - vpsC (VC0919) and vpsG (VC0923) - are predicted to encode serine acetyltransferase-related proteins that may be involved in VPS O-acetylation. The presence of GlcNAc is consistent with our previous report showing that...”
- Role of Vibrio polysaccharide (vps) genes in VPS production, biofilm formation and Vibrio cholerae pathogenesis
Fong, Microbiology (Reading, England) 2010 - “...genes expressed during infection revealed that several vps genes, namely vpsA (VC0917), vpsB (VC0918), vpsC (VC0919) and vpsN (VC0936), are expressed during human infection ( Lombardo et al. , 2007 ). In vivo -induced antigen technology (IVIAT) has also shown that sera from cholera patients recognize...”
- “...N -acetylglucosamine 2-epimerase VC0918 vpsB 413 + I UDP- N -acetyl- d -mannosaminuronic acid dehydrogenase VC0919 vpsC 184 + + +++ +++ IV Acetyltransferase VC0920 vpsD 382 + II Glycosyltransferase VC0921 vpsE 469 + III Polysaccharide export VC0922 vpsF 406 + VI Hypothetical protein VC0923 vpsG...”
- Identification and characterization of OscR, a transcriptional regulator involved in osmolarity adaptation in Vibrio cholerae
Shikuma, Journal of bacteriology 2009 - “...2.99 2.46 Biofilm matrix production VC0916 VC0917 VC0918 VC0919 VC0922 VC0924 VC0925 VC0926 VC0928 VC0930 VC0932 VC0933 VC0935 VC0936 VC1888 vpsU vpsA vpsB vpsC...”
- Interplay between cyclic AMP-cyclic AMP receptor protein and cyclic di-GMP signaling in Vibrio cholerae biofilm formation
Fong, Journal of bacteriology 2008 - “...type Biofilm matrix production genes VC0916 VC0917 VC0918 VC0919 VC0922 VC0928 VC0929 VC0930 VC0931 VC0932 VC0933 VC0935 VC0936 VC0937 VC0939 VC0583 VC1888...”
- Genomic and phenotypic diversity of coastal Vibrio cholerae strains is linked to environmental factors
Keymer, Applied and environmental microbiology 2007 - “...2019 by guest VC0198 to VC0203 VC0790 to VC0801 VC0919 to VC0923 Parameter (gene presence)e 3712 KEYMER ET AL. APPL. ENVIRON. MICROBIOL. TABLE 3. Dispensable...”
- Genetic diversity of toxigenic and nontoxigenic Vibrio cholerae serogroups O1 and O139 revealed by array-based comparative genomic hybridization
Pang, Journal of bacteriology 2007 - “...in other genome regions. One gene cluster, VC0919 to VC0925, contains exopolysaccharide (EPS) biosynthesis genes. This region was Downloaded from...”
- “...by using primers for the flanking ORFs of VC0919 to VC0925. Restriction enzyme fragment lengths of PCR products of this region revealed different patterns...”
- More
PP3136 serine O-acetyltransferase, putative from Pseudomonas putida KT2440
PP_3136 serine O-acetyltransferase from Pseudomonas putida KT2440
35% identity, 51% coverage
- New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories
Martínez-García, Frontiers in bioengineering and biotechnology 2014 - “...bolA , BolA family protein KT-S1 ME-I 4,463,594 PP3956 + Hypothetical protein KT-S2 ME-I 3,552,337 PP3136 NA Intergenic region (PP3136 and PP3137) KT-S3 ME-I 4,671,241 PP4132 Hypothetical protein KT-S4 ME-I 35,484 PP0031 Hypothetical protein KT-S5 ME-O 2,422,455 PP2123 + moeA , molybdopterin biosynthesis enzyme KT-S6 ME-O...”
- Colony morphology and transcriptome profiling of Pseudomonas putida KT2440 and its mutants deficient in alginate or all EPS synthesis under controlled matric potentials
Gulez, MicrobiologyOpen 2014 - “...N/A PP_3134 Conserved domain protein 1.87 2.65 N/A PP_3135 Glycosyl transferase, putative 1.42 2.22 N/A PP_3136 Serine O-acetyltransferase, putative 0.71 1.01 N/A PP_3137 Glycosyl transferase, group 2 family protein 0.42 0.69 N/A PP_3138 VirK domain protein 1.65 1.78 N/A PP_3139 Glycosyl transferase, group 1 family protein...”
PARA_02820 serine O-acetyltransferase from Haemophilus parainfluenzae T3T1
36% identity, 42% coverage
STM2114 putative acyl transferase in colanic acid biosynthesis from Salmonella typhimurium LT2
35% identity, 48% coverage
HMPREF1322_RS09085 serine O-acetyltransferase from Porphyromonas gingivalis W50
PG0115 hexapeptide transferase family protein from Porphyromonas gingivalis W83
34% identity, 52% coverage
- Prediction of Selected Biosynthetic Pathways for the Lipopolysaccharide Components in Porphyromonas gingivalis
Swietnicki, Pathogens (Basel, Switzerland) 2021 - “...protein HMPREF1322_0450 glycosyltransferase family 1 protein HMPREF1322_0451 hypothetical protein HMPREF1322_0454 aminoglycoside 3-N-acetyltransferase HMPREF1322_0455 hypothetical protein HMPREF1322_RS09085 serine acetyltransferase HMPREF1322_0456 hypothetical protein HMPREF1322_0457 hypothetical protein HMPREF1322_0458 glycosyltransferase family 2 protein HMPREF1322_0459 glycosyltransferase HMPREF1322_0460 UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) a Gene assignment was based on the PG0106 -> PG0120 operon...”
- Prediction of Selected Biosynthetic Pathways for the Lipopolysaccharide Components in Porphyromonas gingivalis
Swietnicki, Pathogens (Basel, Switzerland) 2021 - “...( PG0110 , PG0111 , PG0118 , and PG0119 ), two acetyltransferases ( PG0113 and PG0115 ), and a flippase ( PG0117 ). Since the structure of the antigen is not known, it is not possible to assign specific functions to most of the enzymes, except...”
- HcpR of Porphyromonas gingivalis is required for growth under nitrosative stress and survival within host cells
Lewis, Infection and immunity 2012 - “...PG1617 PG0829 PG1240 PG1149 PG0742 PG1688 PG0115 Ferric uptake transcriptional regulator Conserved hypothetical protein Hypothetical protein Hypothetical...”
- Identification and characterization of the capsular polysaccharide (K-antigen) locus of Porphyromonas gingivalis
Aduse-Opoku, Infection and immunity 2006 - “...(PG0120), acetyltransferase (PG0113), serine acetyltransferase (PG0115), UDP-Glc (GDP-Man) dehydrogenase (PG0108), an undecaprenyl phosphate GlcNAc transferase...”
- “...proteins (PG0112, PG0114, and PG0116), serine acetyltransferase (PG0115), a FIG. 2. Analysis of supernatants of autoclaved cells of P. gingivalis parent...”
- Complete genome sequence of the oral pathogenic Bacterium porphyromonas gingivalis strain W83
Nelson, Journal of bacteriology 2003 - “...PG0072 PG0076 PG0106 PG0108 PG0110 PG0111 PG0112 PG0113 PG0115 PG0117 PG0118 PG0119 PG0120 PG0129 PG0139 PG0264 PG0294 PG0311 PG0334 PG0352 PG0369 PG0435 PG0436...”
ETAE_1960 serine acetyltransferase-related protein from Edwardsiella tarda EIB202
37% identity, 44% coverage
WcaB / b2058 colanic acid biosynthesis acetyltransferase WcaB from Escherichia coli K-12 substr. MG1655 (see 6 papers)
wcaB / P0ACC9 colanic acid biosynthesis acetyltransferase WcaB from Escherichia coli (strain K12) (see 5 papers)
b2058 predicted acyl transferase from Escherichia coli str. K-12 substr. MG1655
P0ACC9 Putative colanic acid biosynthesis acetyltransferase WcaB from Escherichia coli (strain K12)
ECs2863 putative transferase from Escherichia coli O157:H7 str. Sakai
33% identity, 48% coverage
- Regulation of cell division, biofilm formation, and virulence by FlhC in Escherichia coli O157:H7 grown on meat
Sule, Applied and environmental microbiology 2011 - “...0.35 0.10 0.0047 4.03 109 Biofilm (2) b0196 b2058 rcsF* wcaB* Regulator in colanic acid synthesis Putative transferase 0.38 0.44 0.0025 0.0087 Miscellaneous...”
- Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli
Reid, Biochemistry 2021 - “...P71241, WcaI: P32057, WcaE: P71239, WcaC: P71237, WcaA: P77414, WcaL: P71243, WcaF: P0ACD2 , WcaB: P0ACC9, WcaK: P71242, WcaM: P71244, WzxC: P77377, WcaD: P71238 ABBREVIATIONS BPP Bactoprenyl diphosphate BP Bactoprenyl phosphate Rcs Regulation of capsule synthesis SIM Selected ion monitoring REFERENCES [1] Whitfield C , and...”
- Identification of the Functional Roles of Six Key Proteins in the Biosynthesis of Enterobacteriaceae Colanic Acid
Scott, Biochemistry 2019 - “...Cps2E: Q9ZII5, WcaI: P32057, WcaE: P71239, WcaC: P71237, WcaA: P77414, WcaL: P71243, WcaF: P0ACD2, WcaB: P0ACC9, WcaK: P71242, WcaM: P71244 Funding Sources Funding supporting this work was received through grants from the National Institutes of Health General Medical Sciences grant numbers: R01GM123251 and R15GM114773. Supporting Information...”
- Physiological Response of Escherichia coli O157:H7 Sakai to Dynamic Changes in Temperature and Water Activity as Experienced during Carcass Chilling
King, Molecular & cellular proteomics : MCP 2016 - “..., wcaF / ECs2859 , wcaE / ECs2860 , wcaC / ECs2862 , wcaB / ECs2863 , wcaA / ECs2864 , wzc / ECs2865, and yjbF / ECs5010 ). Cluster I also contained a number of genes involved in energy metabolism, including genes of the TCA...”
PGN_0230 probable serine acetyltransferase from Porphyromonas gingivalis ATCC 33277
35% identity, 52% coverage
BPSL2775 putative colanic acid biosynthesis acetyltransferase from Burkholderia pseudomallei K96243
32% identity, 53% coverage
- Predicting toxins found in toxin-antitoxin systems with a role in host-induced Burkholderia pseudomallei persistence
Ross, Scientific reports 2020 - “...toxins described in the next section and not previously investigated. Of the toxins BPSL0034, BPSL1494, BPSL2775, BPSL2851, BPSS0899, BPSS0698, BPSS0899, BPSS1321, and BPSS2196 that were tested, 75% of the over-expressed genes led to a reduction in growth, indicating their functionality (Supplemental Figure S2 ). Figure 1...”
- “...BPSL1494 100.00*** 383.08*** 100.00*** 26.34** BPSS1321 100.00*** 100.00*** 14.29* 0.00* BPSS0899 100.59*** 100.00*** 185.71*** 134.16*** BPSL2775 100.30*** 1.54* 100.00*** 1.65* BPSS2196 366.03 327.69 450.00 302.06 Cluster 2 BPSS1816 100.00 0.00 100.00 0.00 BPSL2527 100.59 100.00 232.14 102.06 BPSS1014 100.44 100.00 100.00 59.67** BPSS1584 99.41 290.77 189.29...”
- Transcriptomic analysis of longitudinal Burkholderia pseudomallei infecting the cystic fibrosis lung
Price, Microbial genomics 2018 - “...this pattern of upregulation in both CF11 isolates was maintained for CPS-IV loci BPSL2769 and BPSL2775 BPSL2781 even when compared with the initial CF6, CF8, CF9 and CF10 isolates (2.0- to 4.0-fold; 4 to 16). DE of other virulence-associated loci In addition to CPS-I and LPS,...”
PF0796 galactoside o-acetyltransferase from Pyrococcus furiosus DSM 3638
37% identity, 38% coverage
FGSG_10069 hypothetical protein from Fusarium graminearum PH-1
31% identity, 18% coverage
EC042_RS02000 galactoside O-acetyltransferase from Escherichia coli 042
31% identity, 48% coverage
FOXG_05432 hypothetical protein from Fusarium oxysporum f. sp. lycopersici 4287
35% identity, 17% coverage
- Gene Regulatory Network Inference and Gene Module Regulating Virulence in Fusarium oxysporum
Cano, Frontiers in microbiology 2022 - “...in the GRN. From these analyzes, five TFs were identified as hubs, including FOXG_04688 and FOXG_05432, which regulate 2,404 and 1,864 target genes, respectively. In addition, 16 communities were identified in the GRN, where the largest contains 1,923 genes and the smallest contains 227 genes. Finally,...”
- “...Betweenness centrality Eigenvector centrality 1 FOXG_04688 (0.2382739) * FOXG_14504 (0.00538830) FOXG_04688 (0.001342) FOXG_02500 (0.05238574) 2 FOXG_05432 (0.18485237) FOXG_08042 (0.00537961) FOXG_05432 (0.0010579) FOXG_03418 (0.05093478) 3 FOXG_01037 (0.11987755) FOXG_02500 (0.00537207) FOXG_01037 (0.0009947) FOXG_01626 (0.0498954) 4 FOXG_04255 (0.0987459) FOXG_12790 (0.00530916) FOXG_05265 (0.0009814) FOXG_00928 (0.0498882) 5 FOXG_05265 (0.09045126) FOXG_03680 (0.0052953)...”
Lreu_0613 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase from Limosilactobacillus reuteri subsp. reuteri
Lreu_0613 tetrahydrodipicolinate succinyltransferase domain-containing protein from Lactobacillus reuteri DSM 20016
30% identity, 53% coverage
- In Silico Genomic and Metabolic Atlas of Limosilactobacillus reuteri DSM 20016: An Insight into Human Health
Smythe, Microorganisms 2022 - “...connections can be made. While reviewing this pathway through UniProt, it was noted that gene Lreu_0613 was labeled as 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase in the KEGG database but as 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase in the UniProt database. The almost identical strain L. reuteri JCM 1112 had an identical amino acid...”
- Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri
Santos, Applied and environmental microbiology 2009 - “...lreu_0445 lreu_0502 lreu_0610 lreu_0611 lreu_0612 lreu_0613 lreu_1553 lreu_1791 lreu_1792 Succinyl-diaminopimelate desuccinylase (EC 3.5.1.18) Cystathionine...”
XC_3619 acetyl transferase/isomerase from Xanthomonas campestris pv. campestris str. 8004
34% identity, 36% coverage
- PilG and PilH antagonistically control flagellum-dependent and pili-dependent motility in the phytopathogen Xanthomonas campestris pv. campestris
Qi, BMC microbiology 2020 - “...assay (Fig. 5 c). PilG confirmed directly interaction with XC_0940, XC_1061, XC_1163, XC_1359, XC_2284 and XC_3619. When PilH was tested it showed interactions with XC_1050, XC_3262, XC_1185, XC_1355, XC_1378, XC_3597 and XC_4031. Interestingly, both PilG and PilH proteins interacted with FilN by using bacterial two hybrid...”
- “...the MS-coupled co-IP experiments. We found that PilG could interact with PilN, PilR, PilU, CheA, XC_3619 and XC_1163. While PilH interacted with PilI, ColS, PhoP, XC_1355, XC_1378, XC_3262 and XC_3597. Importantly, our previous work has shown that mutation of genes encoding PilN (XC_0940), PilU (XC_1359), PilR...”
1kruA / P07464 Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
31% identity, 48% coverage
- Ligands: coenzyme a; 1-methylethyl 1-thio-beta-d-galactopyranoside (1kruA)
LacA / b0342 galactoside O-acetyltransferase (EC 2.3.1.18) from Escherichia coli K-12 substr. MG1655 (see 2 papers)
lacA / P07464 galactoside O-acetyltransferase (EC 2.3.1.18) from Escherichia coli (strain K12) (see 19 papers)
THGA_ECOLI / P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 5 papers)
P07464 galactoside O-acetyltransferase (EC 2.3.1.18) from Escherichia coli (see paper)
NP_414876 galactoside O-acetyltransferase from Escherichia coli str. K-12 substr. MG1655
b0342 galactoside O-acetyltransferase from Escherichia coli str. K-12 substr. MG1655
31% identity, 48% coverage
- function: Catalyzes the CoA-dependent transfer of an acetyl group to the 6-O-methyl position of a range of galactosides, glucosides, and lactosides (PubMed:14009486, PubMed:4630409, PubMed:7592843). May assist cellular detoxification by acetylating non-metabolizable pyranosides, thereby preventing their reentry into the cell (Probable).
catalytic activity: a beta-D-galactoside + acetyl-CoA = a 6-acetyl-beta-D- galactoside + CoA (RHEA:15713)
subunit: Homotrimer. - The amino acid sequence of thiogalactoside transacetylase of Escherichia coli.
Fowler, Biochimie 1985 (PubMed)- GeneRIF: N-terminus verified by Edman degradation on complete protein
- Quantitative Shotgun Proteomic Analysis of Bacteria after Overexpression of Recombinant Spider Miniature Spidroin, MaSp1
Randene, International journal of molecular sciences 2024 - “...and lactose metabolism. Gene Protein Acc. # FCR p -Value Function lacA Galactoside O-acetyltransferase * P07464 +OLR 7.89 10 19 Lactose metabolism lacZ Beta-galactosidase * P00722 +OLR +270.33 1.00 10 20 Lactose metabolism N/A MaSp1 NTD-2x-CTD N/A +OLR +12.09 1.00 10 20 Target protein ybgl GTP...”
- “...= fold-change ratio. Gene Protein Acc. # p -Value FCR Function lacA Galactoside O-acetyltransferase * P07464 1.03 10 14 +OLR Lactose metabolism qorB Quinone oxidoreductase 2 P39315 8.38 10 4 +OLR Electron transport in respiratory chain cnoX Chaperedoxin P77395 8.38 10 4 +OLR Protein deaggregation dadX...”
- A Continuous Assay Set to Screen and Characterize Novel Protein N-Acetyltransferases Unveils Rice General Control Non-repressible 5-Related N-Acetyltransferase2 Activity
Asensio, Frontiers in plant science 2022 - “...bring unambiguous answer through the PDH-coupled assay. The sequence was derived from galactoside O-acetyltransferase (THGA, P07464). We could verify that the derived N-terminal decapeptide was indeed a substrate of OsGNAT2 with catalytic efficiency similar to the other decapeptides ( Table 2 ). We reported that plastid...”
- Identification of a Novel Therapeutic Target against XDR Salmonella Typhi H58 Using Genomics Driven Approach Followed Up by Natural Products Virtual Screening
Jalal, Microorganisms 2021 - “...1 Colanic acid biosynthesis acetyltransferase Serine acetyltransferase Q0WKM4 DB02078 Serine acetyltransferase P43886 DB01992 Galactoside o-acetyltransferase P07464 DB01862 2 Shikimate dehydrogenase Shikimate dehydrogenase P15770 DB03461; DB04447 Shikimate dehydrogenase P43876 DB02363 3 Pantoate-beta-alanine ligase Pantothenate synthetase Q5SHF5 DB03570 Pantothenate synthetase P0A5R0 DB01930; DB02596 4 Multidrug efflux RND transporter...”
- Analysis of a capsular polysaccharide biosynthesis locus of Bacteroides fragilis
Comstock, Infection and immunity 1999 - “...3-362) 1-364) 10-100) 10-236) D55213 P55254 S23343 P37745 P07464 P39856 G47677 S23344 U46859 AB004860 Q46634 U46859 U73857 U73857 A47677 Q48457 X85787 AF004325...”
- A workflow for annotating the knowledge gaps in metabolic reconstructions using known and hypothetical reactions
Vayena, Proceedings of the National Academy of Sciences of the United States of America 2022 - “...alternative pathway to compensate for the knockout of argG (b3172). The second added gene, LacA (b0342), encodes the enzyme galactoside O-acetyltransferase, which catalyzes the transfer of an acetyl group from acetyl-CoA to the 6-hydroxyl of some galactopyranosides ( 27 ). This enzyme is known to act...”
- Physiological studies of Escherichia coli strain MG1655: growth defects and apparent cross-regulation of gene expression
Soupene, Journal of bacteriology 2003 - “...level of expression of the lac operon (b0344 to b0342) (but see below). In addition, it resulted in elevated levels of expression of the nagBACD (b0678 to...”
- “...11, 2017 by University of California, Berkeley b0344 b0343 b0342 GROWTH DEFECTS OF E. COLI MG1655 VOL. 185, 2003 5619 TABLE 4. Activity of -amylase in MG1655...”
- DNA microarray analyses of the long-term adaptive response of Escherichia coli to acetate and propionate
Polen, Applied and environmental microbiology 2003 - “...1.84* 1.63* 2.10* 2.14* 2.07* 0.78 0.94 0.87 b0342 b0343 b0344 lacA lacY lacZ 1 1 1 Thiogalactoside acetyltransferase Galactoside permease (M protein)...”
- Genome-wide expression profiling in Escherichia coli K-12
Richmond, Nucleic acids research 1999 - “...by IPTG Bnumbera Nameb Radioactivec Microarrayd Gene producte b0342 lacA 79 43 Thiogalactoside acetyltransferase b0344 lacZ 62 49 -D-galactosidase b0343 lacY 55...”
Rfer_0690 WxcM-like from Rhodoferax ferrireducens DSM 15236
34% identity, 37% coverage
Q6F4D7 serine O-acetyltransferase (EC 2.3.1.30) from Thermus thermophilus HB8 (see paper)
30% identity, 39% coverage
SPD_1923 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase, putative from Streptococcus pneumoniae D39
spr1907 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase-related protein from Streptococcus pneumoniae R6
32% identity, 37% coverage
- Dynamic Pneumococcal Genetic Adaptations Support Bacterial Growth and Inflammation during Coinfection with Influenza
Smith, Infection and immunity 2021 - “...protein SPD_1354 Conserved hypothetical protein SPD_1414 Oxalate:formate antiporter SPD_1468 Phosphoglycerate mutase SPD_1782 ksgA Dimethyladenosine transferase SPD_1923 2,3,4,5-Tetrahydropyridine-2-carboxylate N -succinyltransferase, putative SPD_2047 cbiO1 Cobalt ABC transporter, ATP-binding protein CbiO1 SPD_2048 cbiO2 Cobalt ABC transporter, ATP-binding protein CbiO2 Total 17 23 a Pneumococcal fitness genes identified at 12...”
- Inactivation of Transcriptional Regulator FabT Influences Colony Phase Variation of Streptococcus pneumoniae
Zhang, mBio 2021 - “...mutS DNA mismatch repair protein MutS 0.54 0.041 SPD_1922 hipO N -acetyldiaminopimelate deacetylase 0.59 0.043 SPD_1923 dapD 2,3,4,5-Tetrahydropyridine-2,6-dicarboxylate N -acetyltransferase 0.85 0.004 SPD_1926 tyrS Tyrosine-tRNA ligase 1.07 0.002 SPD_1927 ctpC Heavy metal translocating P-type ATPase 0.96 0.000 SPD_1931 Membrane protein 0.74 0.020 SPD_1984 ybbK Putative stomatin/prohibitin-family...”
- Redefining the Small Regulatory RNA Transcriptome in Streptococcus pneumoniae Serotype 2 Strain D39
Sinha, Journal of bacteriology 2019 - “...1873276-1873324 Spd_sr106b 1892400-1892550 () spd_1924, spd_1923 Spd_sr107 Spd_sr108b Spd_sr109b 1903127-1903247 () malP, spd_1931 1913211-1913442 () spd_1939,...”
- Reactive Oxygen Species Contribute to the Bactericidal Effects of the Fluoroquinolone Moxifloxacin in Streptococcus pneumoniae
Ferrándiz, Antimicrobial agents and chemotherapy 2016 - “...acid biosynthesis: Gln Amino acid biosynthesis: Ser spr1907 (dapD) spr0443c-0444 (glnRA) spr1095 (metY) spr0823 (but)d spr1324 (apbE)d spr0530 (fba) spr1902...”
SP_2097 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase, putative from Streptococcus pneumoniae TIGR4
32% identity, 37% coverage
A8AUL9 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase from Streptococcus gordonii (strain Challis / ATCC 35105 / BCRC 15272 / CH1 / DL1 / V288)
SGO_0158 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase, putative from Streptococcus gordonii str. Challis substr. CH1
32% identity, 37% coverage
- Intracellular Ser/Thr/Tyr phosphoproteome of the oral commensal Streptococcus gordonii DL1
Robertsson, BMC microbiology 2020 - “...dehydrogenase ald (SGO_0708) A8AW50 6.1/5.29 45,000/38960 1378 19 63% daph a) 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase dapH (SGO_0158) A8AUL9 5.2/5.06 22,000/24255 291 5 25% glya Serine hydroxymethyltransferase glyA, (SGO_1151) A8AXC8 5.4/5.07 48,000/45566 583 8 28% sed Homoserine dehydrogenase hom (SGO_0801) A8AWE0 5.0/4.86 45,000/46154 245 3 11% Nucleotide metabolism adk...”
- Intracellular Ser/Thr/Tyr phosphoproteome of the oral commensal Streptococcus gordonii DL1
Robertsson, BMC microbiology 2020 - “...Alanine dehydrogenase ald (SGO_0708) A8AW50 6.1/5.29 45,000/38960 1378 19 63% daph a) 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase dapH (SGO_0158) A8AUL9 5.2/5.06 22,000/24255 291 5 25% glya Serine hydroxymethyltransferase glyA, (SGO_1151) A8AXC8 5.4/5.07 48,000/45566 583 8 28% sed Homoserine dehydrogenase hom (SGO_0801) A8AWE0 5.0/4.86 45,000/46154 245 3 11% Nucleotide metabolism...”
c0457 Galactoside O-acetyltransferase from Escherichia coli CFT073
34% identity, 40% coverage
PSPTO1070 lipopolysaccharide biosynthesis protein from Pseudomonas syringae pv. tomato str. DC3000
33% identity, 36% coverage
- Protein domains and architectural innovation in plant-associated Proteobacteria
Studholme, BMC genomics 2005 - “...include hrpZ , PSPTO3210, glf , PSPTO4696, hopPtoS (1,2 & 3), PSPTO2259, PSPTO0400, avrF and PSPTO1070. The GC content of flanking genes frequently reflected that of the novel gene, most strikingly for glf , PSPTO2441, PSPTO4696, hopPtoS (1,2 &3), PSPTO4699, PSPTO1070 & PSPTO2632, which were each...”
ECs0395 thiogalactoside acetyltransferase from Escherichia coli O157:H7 str. Sakai
34% identity, 40% coverage
B2RGM6 UDP-2-acetamido-3-amino-2,3-dideoxy-glucuronate N-acetyltransferase (EC 2.3.1.201) from Porphyromonas gingivalis (see paper)
PGN_0002 hypothetical protein from Porphyromonas gingivalis ATCC 33277
33% identity, 41% coverage
- PGN_0297 is an essential component of the type IX secretion system (T9SS) in Porphyromonas gingivalis: Tn-seq analysis for exhaustive identification of T9SS-related genes
Naito, Microbiology and immunology 2019 - “...T9SSrelated genes previously reported Locus No. of Tn mutant clones Gene name Product Reference 1 PGN_0002 5 wbpD Wbp pathway protein WbpD 29 2 PGN_0022 38 porU T9SS component protein PorU 2 3 5intergenic region of PGN_0023 1 porV/pg27/lptO T9SS component protein PorV/Pg27/LptO 18 , 19...”
- Porphyromonas gingivalis and related bacteria: from colonial pigmentation to the type IX secretion system and gliding motility
Nakayama, Journal of periodontal research 2015 - “...Wbp pathway, which involves WbpA (PGN_0613 [UgdA], PGN_1243), WbpB (PGN_0168), WbpE (PGN_1236 [PorR]) and WbpD (PGN_0002), is a sugar substrate required for the biosynthesis of A-LPS ( 38 ). The P. gingivalis strain HG66, typically used for gingipain purification, exhibits no pigmentation on blood agar. This...”
- Involvement of the Wbp pathway in the biosynthesis of Porphyromonas gingivalis lipopolysaccharide with anionic polysaccharide
Shoji, Scientific reports 2014 - “...the WbpA, WbpB, WbpE, WbpD and WbpI proteins. We then determined the effect of the PGN_0002 gene, a wbpD homolog, on the biosynthesis of A-LPS. A PGN_0002-deficient mutant demonstrated an A-LPS biosynthesis deficiency. Taken together with previous studies, the present results suggest that the final product...”
- “...wbpA homologs, wbpB (PGN_0168) was a wbpB homolog, porR (PGN_1236) was a wbpE homolog, and PGN_0002 was a wbpD homolog; no wbpI homologs were found. Our study revealed that the A-LPS deficiency of strain HG66 was the result of a nonsense mutation in the wbpB gene,...”
NP_347413 Acetyltransferase (the isoleucine patch superfamily) from Clostridium acetobutylicum ATCC 824
49% identity, 20% coverage
- Rickettsia phylogenomics: unwinding the intricacies of obligate intracellular life
Gillespie, PloS one 2008 - “...QCD-32g58; HP CdifQ_04002574 50.1 3.00E-05 NP_246134 Pasteurella multocida subsp. multocida str. Pm70; VatB 50.1 3.00E-05 NP_347413 Clostridium acetobutylicum ATCC 824; Acetyltransferase (the Ile patch superfamily) 50.1 4.00E-05 YP_083964 Bacillus cereus E33L; virginiamycin A acetyltransferase 50.1 4.00E-05 YP_734654 Shewanella sp. MR-4; streptogramin A acetyl transferase 50.1 4.00E-05...”
PG0002 hexapeptide transferase family protein from Porphyromonas gingivalis W83
PG_0002 acyltransferase from Porphyromonas gingivalis W83
32% identity, 41% coverage
lpg0754 acetyltransferase from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
36% identity, 38% coverage
- Small Regulatory RNA and Legionella pneumophila
Faucher, Frontiers in microbiology 2011 - “...lpr0021 744637 744929 292 lpg0691 Weissenmayer et al. ( 2011 ) lpr0025 825408 825265 143 lpg0754 Weissenmayer et al. ( 2011 ) lpr0026 837669 837871 202 lpg0766 Weissenmayer et al. ( 2011 ) lpr0028 871501 871409 92 lpg0796 Weissenmayer et al. ( 2011 ) lpr0029 1046275...”
- Identification of Legionella pneumophila-specific genes by genomic subtractive hybridization with Legionella micdadei and identification of lpnE, a gene required for efficient host cell entry
Newton, Infection and immunity 2006 - “...E10/2B5 2E6 2C12 G7 C3, B5 D12, E12 yvf, lpg0754, waaM, lpg0774 LPS and lipidA biosynthesis lpg2699 lepB lpg2222 ladC lpg2977, lpg1176 pleD, lpg1357 Autolysin...”
- “...and B11, representing neighboring orf21 (lyrF) and orf22 (lpg0754), were L. pneumophila specific. This piece of data is consistent with a recent hybridization...”
PARA_02860 acetyltransferase from Haemophilus parainfluenzae T3T1
24% identity, 50% coverage
- Haemophilus parainfluenzae expresses diverse lipopolysaccharide O-antigens using ABC transporter and Wzy polymerase-dependent mechanisms
Young, International journal of medical microbiology : IJMM 2013 - “...2004 ). Putative nnaA nnaD genes are present within the T3T1 OAg locus ( PARA_02830 PARA_02860 ), sharing high levels of sequence similarity with the genes for polysialic acid capsule biosynthesis in Mannheimia haemolytica serotype A2 strains ( Adlam et al., 1987 ). In addition, PARA_02800...”
- “...The acetylation of the sialic acid residue might be carried out by either NnaD (NeuD; PARA_02860) or the putative O-acetyltransferase PARA_02820. H. influenzae can also decorate its LPS with Neu5Ac, which it obtains from the environment using a tripartite ATP-independent periplasmic (TRAP) transporter encoded by the...”
FNP_1107 acetyltransferase from Fusobacterium polymorphum ATCC 10953
28% identity, 37% coverage
- Ubiquitous sialometabolism present among oral fusobacteria
Yoneda, PloS one 2014 - “...and Lipase DGSL2 family (NeuA) CMP-Neu5Ac synthesis FNP_1106 NeuB family; SAF domain (NeuB) Neu5Ac synthesis FNP_1107 Bacterial transferase hexapeptide; sialic acid O-acetyltransferase (NeuD) Neu5Ac synthesis/modification ssp. vincentii * FNV1764 UDP-N-acetylglucosamine 2-epimerase (NeuC) ManNAc synthesis FNV1765 CTP transferase 3 and Lipase DGSL2 family (NeuA) CMP-Neu5Ac synthesis FNV1766...”
- “...novo sialic acid synthesis capabilities. These three organisms also encode putative neuD sialic acid O-acetyltransferases (FNP_1107, FNV1767, and FPOG_00225) that are likely involved in adding O-acetylation to the sialic acid molecules [43] , [44] . This activity appears to be missing from the ssp. nucleatum neu...”
- Genome sequence of Fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium
Karpathy, PloS one 2007 - “...FNP_1105 1446154 1444898 neuA CMP-N-acetylneuraminate cytidylyltransferase FNP_1106 1447200 1446157 neuB possible N-acetyl neuramic acid synthetase FNP_1107 1447822 1447205 N-acetylneuraminate synthase FNP_1108 1449226 1447934 oligosaccharidyl-lipid/polysaccharide flippase FNP_1109 1450280 1449300 possible lipooligosaccharide sialyltransferase FNP_1205 1556498 1557478 waaE possible ADP-heptose synthase FNP_1807 2161658 2162707 waaF2 LPS heptosyltransferase II FNP_1808...”
WP_011172737 acyltransferase from Thermus thermophilus
29% identity, 37% coverage
7s3uA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d-glucose (see paper)
34% identity, 38% coverage
- Ligands: coenzyme a; [(3r,4s,5s,6r)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2r,3s,5r)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate (7s3uA)
EHI_096770 acetyltransferase, putative from Entamoeba histolytica HM-1:IMSS
37% identity, 34% coverage
- Screening and Structural Characterization of Heat Shock Response Elements (HSEs) in <i>Entamoeba histolytica</i> Promoters
Dorantes-Palma, International journal of molecular sciences 2024 - “...elements, either individually or in combination. Prominent in this group are genes such as acetyltransferase (EHI_096770), Acetyltransferase GNAT family (EHI_137930), and Serine O-acetyltransferase (EHI_021570), all involved in acetyl group transfer, and Vacuolar protein sorting 26A (EHI_162540), which plays a role in protein folding ( Supplementary Table...”
- “...with 21 genes, and Transferase (PC00220) with 39 genes. Within this category, genes like Acetyltransferase (EHI_096770), involved in O-acyltransferase activity, and Methionine Gamma-Lyase (EHI_144610), with functions in binding to heterocyclic or cyclic organic compounds and lyase activity, are found. Similar to the first category, Ehpgp5 is...”
- Global analysis of gene expression in response to L-Cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica
Husain, BMC genomics 2011 - “...involved in chitin biosynthetic pathway (Table 1 ). Two other genes encoding putative acetyltransferases (103.m00159, EHI_096770) were also induced 3-5 fold upon L-cysteine deprivation (Table 1 ; Additional file 2 ). These acetyltransferases contain maltose/galactose-O-acetyltransferase domains, and are known to be involved in the acetylation of...”
- “...the pathways that these acetyltransferases are involved in, are not known in E. histolytica . EHI_096770 was also induced upon H 2 O 2 -mediated oxidative (4 fold) or DPTA-NONOate-mediated nitrosative stress (2.7 fold) in E. histolytica [ 23 ]. A gene encoding cyst wall specific...”
4mzuF / Q12KT8 Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
30% identity, 34% coverage
- Ligands: coenzyme a; thymidine-5'-diphosphate; thymine (4mzuF)
Q5HCZ5 galactoside O-acetyltransferase (EC 2.3.1.18) from Staphylococcus aureus (see paper)
SAOUHSC_02871 hypothetical protein from Staphylococcus aureus subsp. aureus NCTC 8325
SAUSA300_2492 acetyltransferase family protein from Staphylococcus aureus subsp. aureus USA300_FPR3757
SACOL2570 galactoside O-acetyltransferase from Staphylococcus aureus subsp. aureus COL
25% identity, 45% coverage
- Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure
Yang, Frontiers in microbiology 2021 - “...2,072,412 20.49 / / Non-coding region 0 g/mL 2,644,158 26.92 F < - > F SAOUHSC_02871 Hypothetical protein 0 g/mL 2,728,755 18.98 V < - > I SAOUHSC_02967 Arginine/ornithine antiporter 0 g/mL 2,748,895 19.82 T < - > S SAOUHSC_02982 Hypothetical protein 2 g/mL 92,210 17.59...”
- “...SNP at position 1942424 is located in SAOUHSC_02036, which encodes a phage structural protein. The SAOUHSC_02871 gene (containing SNP 2644158) encodes an acetyltransferase. FIGURE 3 Manhattan plots of bivariate functional mapping. Manhattan plot of the significance test based on the associations between phenotypic plasticity of microbial...”
- Inferring multilayer interactome networks shaping phenotypic plasticity and evolution
Yang, Nature communications 2021 - “...Q25261 (at SAOUHSC_00020 ), Q193712 (at SAOUHSC_00176 ), Q92210 (at SAOUHSC_00085 ), and Q2644158 (at SAOUHSC_02871 ), have significant net effects mainly due to favorable accumulated promotion from regulators, although the strength of promotion and number of regulators differ from QTL to QTL (Fig. S 4C...”
- Loratadine inhibits Staphylococcus aureus virulence and biofilm formation
Zheng, iScience 2022 - “...C751T R251H (nonsyn) SA113_GM000812 SAOUHSC_00,694 Q2G0B1; HTH-type transcriptional regulator MgrA G275T R92L (nonsyn) SA113_GM001371 SAOUHSC_02,871 Q5HCZ5; Putative acetyltransferase SACOL2570 G412A G138S (nonsyn) SA113_GM002521 SAOUHSC_01,877 O35008; Uncharacterized protein YtqA T786C I262I (syn) Note: S.aureus was serially subcultured in TSB containing500M loratadine. Mutations in two clones (81-1, 81-6)...”
- Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Genetic Factors Involved in Human Endothelial Cells Damage, an Important Phenotype Correlated with Persistent Endovascular Infection
Xiao, Antibiotics (Basel, Switzerland) 2022 - “...compound ABC transporter iron compound-binding protein SirA 11.83 7.84 SAUSA300_0899 mecA adaptor protein 11.58 10.37 SAUSA300_2492 hypothetical acetyltransferase family protein 11.55 7.80 SAUSA300_1433 hypothetical putative phage regulatory protein 11.41 8.17 SAUSA300_1244 mscL large conductance mechanosensitive channel protein 11.32 7.21 SAUSA300_0049 hypothetical hypothetical protein 11.30 0.62 SAUSA300_1667...”
- Cigarette Smoke Extract-Exposed Methicillin-Resistant Staphylococcus aureus Regulates Leukocyte Function for Pulmonary Persistence
Kulkarni, American journal of respiratory cell and molecular biology 2016 - “...SAUSA300_0889 SAUSA300_2175 SAUSA300_0076 SAUSA300_2617 SAUSA300_2492 SAUSA300_2638 SAUSA300_0206 SAUSA300_0784 SAUSA300_2326 SAUSA300_0075 SAUSA300_0952...”
- Loratadine inhibits Staphylococcus aureus virulence and biofilm formation
Zheng, iScience 2022 - “...SA113_GM000812 SAOUHSC_00,694 Q2G0B1; HTH-type transcriptional regulator MgrA G275T R92L (nonsyn) SA113_GM001371 SAOUHSC_02,871 Q5HCZ5; Putative acetyltransferase SACOL2570 G412A G138S (nonsyn) SA113_GM002521 SAOUHSC_01,877 O35008; Uncharacterized protein YtqA T786C I262I (syn) Note: S.aureus was serially subcultured in TSB containing500M loratadine. Mutations in two clones (81-1, 81-6) from 81 generations...”
- The emerging roles of GCN5L1 in mitochondrial and vacuolar organelle biology
Wu, Biochimica et biophysica acta. Gene regulatory mechanisms 2021 - “...suggesting that these regions may be involved in substrate binding. A structural analysis of the SACOL2570 acetyltransferase from Staphylococcus aureus determined that the XAT hexapeptide repeat was an integral component of the substrate binding pockets [ 54 ]. In contrast, GCN5L1 has three XAT hexapeptide repeat...”
- “...Zheng H , Chruszcz M , Minor W , Biophysical analysis of the putative acetyltransferase SACOL2570 from methicillin-resistant Staphylococcus aureus , J Struct Funct Genomics , 14 ( 2013 ) 97 108 . 23963951 [55] Kanehisa M , Goto S , Sato Y , Furumichi M...”
- GCN5L1/BLOS1 Links Acetylation, Organelle Remodeling, and Metabolism
Scott, Trends in cell biology 2018 - “...formation of the correct tertiary structure required for this process. A structural analysis of the SACOL2570 acetyltransferase from Staphylococcus aureus determined that the XAT hexapeptide repeat was integral to the folding of the LH domains that form substrate binding pockets, suggesting that XAT hexapeptide repeat may...”
- “...37 19 6689 96 9578552 27 Luo HB 2013 Biophysical analysis of the putative acetyltransferase SACOL2570 from methicillin-resistant Staphylococcus aureus J Struct Funct Genomics 14 3 97 108 23963951 28 Kanehisa M 2012 KEGG for integration and interpretation of large-scale molecular data sets Nucleic Acids Res...”
- Biophysical analysis of the putative acetyltransferase SACOL2570 from methicillin-resistant Staphylococcus aureus
Luo, Journal of structural and functional genomics 2013 - “...functional genomics 1345-711X 1570-0267 23963951 3923901 10.1007/s10969-013-9158-6 NIHMS520349 Article Biophysical analysis of the putative acetyltransferase SACOL2570 from methicillin-resistant Staphylococcus aureus Luo Hai-Bin Department of Molecular Physiology and Biological Physics, University of Virginia, 1340 Jefferson Park Avenue, Charlottesville, VA 22908, USA; School of Pharmaceutical Sciences, Sun Yat-Sen...”
- “...CoA-bound crystal structures of a member of the galactoside acetyltransferase superfamily from methicillin-resistant S. aureus SACOL2570 which was recently shown to be down regulated in S. aureus grown in the presence of fusidic acid, an antibiotic used to treat MRSA infections. SACOL2570 forms a homotrimerin solution,...”
sll1636 ferripyochelin binding protein from Synechocystis sp. PCC 6803
51% identity, 18% coverage
- Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution
Matsusako, Biotechnology for biofuels 2017 - “...Ins 389860 CCC CCCC sll1025 Frameshift, stop codon 86 Hypothetical protein Ins 549361 CGG CGGG sll1636 ( fbp ) Frameshift, stop codon 84 Ferripyochelin-binding protein SNP 755131 CTG TTG sll1924 ( sycrp2 ) LeuLeu 99 cAMP receptor protein Ins 1041211 GGG GGGG slr1233 ( frdA )...”
- Identification of the direct regulon of NtcA during early acclimation to nitrogen starvation in the cyanobacterium Synechocystis sp. PCC 6803
Giner-Lamia, Nucleic acids research 2017 - “...the peroxide stress response transcriptional regulator PerR ( slr1738 ), and the LexA repressor ( sll1636 ). Furthermore, many genes coding for metal importer systems were downregulated, such as the ATPases for copper ( ctaA and pacS ), cobalt ( coaT ) and zinc ( ziaA...”
- Loss of the SPHF homologue Slr1768 leads to a catastrophic failure in the maintenance of thylakoid membranes in Synechocystis sp. PCC 6803
Bryan, PloS one 2011 - “...al ., 2003. An analysis of the adjacent ORFs, Sll1638, encoding a hypothetical protein, and Sll1636, which encodes a ferripyochelin binding protein, using the web-tool NNPP, suggests that each have their own promoter and, are likely to be transcribed independently. Hence, insertional inactivation of Slr1768 should...”
5u2kA / Q5HCZ5 Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
26% identity, 44% coverage
- Ligand: coenzyme a (5u2kA)
PP0385 conserved hypothetical protein from Pseudomonas putida KT2440
30% identity, 51% coverage
MMP0350 Bacterial transferase hexapeptide repeat from Methanococcus maripaludis S2
29% identity, 38% coverage
- Post-Translational Modifications Aid Archaeal Survival
Gong, Biomolecules 2020 - “...identified in H. volcanii [ 86 , 87 ]. In addition, a putative acetyltransferase gene, MMP0350, has been identified in Methanococcus maripaludis , which affects the proper assembly of both archaellum and pili. This suggests that acetylation may also be involved in the motility regulation of...”
- “...S.Y.M. Kanbe M. Chaban B. Aizawa S.-I. Jarrell K.F. Identification of a putative acetyltransferase gene, MMP0350, which affects proper assembly of both flagella and pili in the archaeon Methanococcus maripaludis J. Bacteriol. 2008 190 5300 5307 10.1128/JB.00474-08 18539748 89. Van Wolferen M. Ajon M. Driessen A.J.M....”
- Emerging facets of prokaryotic glycosylation
Schäffer, FEMS microbiology reviews 2017 - “...Me. maripaludis . Among the genes involved in sugar biosynthesis was the putative acetyltransferase gene mmp0350 , which appears to attach an acetyl group to the second sugar of the glycan, a diacetylated glucuronic acid ( VanDyke et al. 2009 ; Jarrell, Jones and Nair 2010...”
- “...is necessary for archaellum assembly ( Siu et al. 2015 ). Methanococcus maripaludis , the mmp0350 - mmp0353 and mmp0357 genes are proposed to be functionally equivalent to the Pseudomonas aeruginosa wbpABEDI genes involved in converting UDP-GlcNAc to UDP-2,3-diacetamido-2,3-dideoxy- d -mannuronic acid, a serotype O5-specific sugar...”
- Evidence that biosynthesis of the second and third sugars of the archaellin Tetrasaccharide in the archaeon Methanococcus maripaludis occurs by the same pathway used by Pseudomonas aeruginosa to make a di-N-acetylated sugar
Siu, Journal of bacteriology 2015 - “...of N-glycan sugars. Mutants carrying deletions in mmp0350, mmp0351, mmp0352, or mmp0353 were nonarchaellated and synthesized archaellins modified with a 1-sugar...”
- “...strain confirmed a glycan with only GalNAc, suggesting mmp0350 to mmp0353 were all involved in biosynthesis of the second sugar (GlcNAc3NAcA). The mmp0357...”
- N-linked glycosylation in Archaea: a structural, functional, and genetic analysis
Jarrell, Microbiology and molecular biology reviews : MMBR 2014 - “...by a glycan comprising a single sugar in the mmp0350, mmp0351, mmp0352, and mmp0353 deletion strains, all of which are nonarchaellated, is seen. The assignment...”
- “...MMP0353 (a dehydrogenase), MMP0351 (an aminotransferase), MMP0350 (an acetyltransferase), and MMP0357 (an epimerase) share substantial sequence similarity with...”
- Identification of genes involved in the biosynthesis of the third and fourth sugars of the Methanococcus maripaludis archaellin N-linked tetrasaccharide
Ding, Journal of bacteriology 2013 - “...to this genetic locus, another cluster of genes (mmp0350 to mmp0359) has been identified and shown by deletion analysis and mass spectrometry to include...”
- “...KF. 2008. Identification of putative acetyltransferase gene, MMP0350, which affects proper assembly of both flagella and pili in the archaeon Methanococcus...”
- Agl16, a thermophilic glycosyltransferase mediating the last step of N-Glycan biosynthesis in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius
Meyer, Journal of bacteriology 2013 - “...first made in Methanococcus maripaludis, where deletion of MMP0350, encoding a putative acetyltransferase, was proposed to be responsible for the acetylation of...”
- S-layer glycoproteins and flagellins: reporters of archaeal posttranslational modifications
Jarrell, Archaea (Vancouver, B.C.) 2010 - “...of the entire fla operon. Other genes identified as involved in the glycan synthesis include MMP0350, the product of which is likely responsible for addition of one of the two acetyl groups found on the second sugar [ 117 ] and MMP1085 which encodes a protein...”
- “...the native N-linked glycan [ 116 ]. Finally, deletion of a single gene, that is, MMP0350 , assigned as an acetyltransferase necessary for the biosynthesis of the second sugar of the M. maripaludis N-linked glycan, resulted in defects in both flagellation and piliation. Since the glycan...”
- Biosynthesis and role of N-linked glycosylation in cell surface structures of archaea with a focus on flagella and s layers
Jarrell, International journal of microbiology 2010 - “...The first major finding reported in the biosynthetic pathway using this technique was the gene MMP0350. The gene, which shows strong sequence similarity to known acetyltransferases, appears to attach an acetyl group to the second sugar of the glycan, a diacetylated glucuronic acid. Lack of this...”
- “...transferring the second sugar or in aglB, is nonflagellated [ 19 ]. An acetyltransferase gene, MMP0350, likely responsible for addition of an acetyl group to the second sugar (the diacetylated glucuronic acid) [ 19 , 128 ], results in flagellin with apparently a single sugar added,...”
- More
SA2342 hypothetical protein from Staphylococcus aureus subsp. aureus N315
26% identity, 45% coverage
MW1285 tetrahydrodipicolinate acetyltransferase from Staphylococcus aureus subsp. aureus MW2
Q7A2S0 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase from Staphylococcus aureus (strain Mu50 / ATCC 700699)
SA1229 tetrahydrodipicolinate acetyltransferase from Staphylococcus aureus subsp. aureus N315
SAV1397 tetrahydrodipicolinate acetyltransferase from Staphylococcus aureus subsp. aureus Mu50
SAUSA300_1290 tetrahydrodipicolinate acetyltransferase from Staphylococcus aureus subsp. aureus USA300_FPR3757
SACOL1432 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase from Staphylococcus aureus subsp. aureus COL
E5491_RS07270, SAPIG1398, USA300HOU_RS07115 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase from Staphylococcus aureus subsp. aureus ST398
30% identity, 44% coverage
- The SaeR/S gene regulatory system is essential for innate immune evasion by Staphylococcus aureus
Voyich, The Journal of infectious diseases 2009 - “...A 2.25 ES MW1283 dapA dihydrodipicolinate synthase 2.37 ES MW1284 dapB dihydrodipicolinate reductase 2.28 ES MW1285 dapD tetrahydrodipicolinate acetyltransferase 2.15 ES MW1286 - hippurate hydrolase 2.24 ES MW1517 glyS glycyl-tRNA synthetase 2.08 ES MW1721 - transaldolase 2.01 ES MW1842 - glutamyl-tRNAGln amidotransferase subunit C 2.43 ES...”
- Genetic and metabolic signatures of Salmonella enterica subsp. enterica associated with animal sources at the pangenomic scale
Vila, BMC genomics 2019 - “...IlvY 4,116,897 c.317C>A p.Leu106Gln P0A2Q2 Fish Gene 2.310 8 dapH 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase N/A N/A N/A Q7A2S0 Fish Gene 3.310 3 cgkA Kappa-carrageenase N/A N/A N/A P43478 Metabolic pathways mainly impacted by mutations associated with animal sources (i.e. GOEA) Based on the mutations associatedby microbial GWAS (Table...”
- “...Q7CPA1 SNP in aspA GO:0008797 aspartate ammonia-lyase activity 1 0.01 6 110 7 MF fish Q7A2S0 gene dapH GO:0047200 tetrahydrodipicolinate N-acetyltransferase activity 1 0.01 8 110 7 MF fish P43478 gene cgkA GO:0033918 kappa-carrageenase activity 1 0.01 6 110 7 MF Discussion Restricted and unrestricted animal...”
- Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
Anderson, FEMS immunology and medical microbiology 2010 - “...6.0 2.5 ND sarZ SA2384 staphylococcal accessory protein Z sa_c1214s994_a_at * 5.8 2.5 15 sun SA1229 Sun protein sa_c10502s10951cv_s_at * 2.8 2.5 2.5 tcaR SA2353 transcriptional regulator sa_c791s594_at * 8.1 2.5 stable SA1107 transcriptional regulator sa_c3623s3103_a_at * 8.7 2.5 2.5 SA1904 putative transcriptional regulator sa_c446s279_a_at *...”
- “...* 7.7 2.5 ND sarY SA2289 staphylococcal accessory regulator sa_c1214s994_a_at * 7.5 2.5 2.5 sun SA1229 Sun protein sa_c10502s10951cv_s_at 3.6 2.5 2.5 tcaR SA2353 transcriptional regulator sa_c3928s3395_a_at 2.2 2.5 ND SA0179 phosphosugar-binding transcriptional regulator sa_c7020s6143_a_at 2.9 2.5 ND SA0404 transcriptional regulator sa_c7120s6232_a_at 3.4 2.5 ND SA0432...”
- Transcriptional signature following inhibition of early-stage cell wall biosynthesis in Staphylococcus aureus
O'Neill, Antimicrobial agents and chemotherapy 2009 - “...SA1163 SA1164 SA1165 SA1166 SA1170 SA1226 SA1227 SA1228 SA1229 SA1230 SA1231 SA1235 SA1236 SA1238 SA1254 SA1255 SA1256 SA1257 SA1532 SA1545 SA1546 SA1691 SA1858...”
- Microarray analysis of toxicogenomic effects of ortho-phenylphenol in Staphylococcus aureus
Jang, BMC genomics 2008 - “...Decisively SA1225 ( lys C)-SA1226 ( asd )-SA1227 ( dap A)-SA1228 ( dap B) and SA1229 ( dap D) show fold highest decreases as -54.6, -21.5, -27.3, -31.4, and -23.5 folds at 20 min and -7.7, -4.3, -5.2, -5.1, and -4.4 folds at 60 min in...”
- “...CTT GGG TCA GTG GCA TAC ACA-3' 5'-TGG GTG CAA CAG GAT TAG TAG GCA-3' SA1229 -23.5 -4.4 -7.4( 2.0) -1.2( 0.2) 5'-TGG GTG CAA CAG GAT TAG TAG GCA-3' 5'-TTC AAC TTC TTG CCC TGC AGA ACG-3' SA2093 6.8 9.0 15.7( 1.2) 4.6( 0.5) 5'-TTC...”
- Characterization of the oxygen-responsive NreABC regulon of Staphylococcus aureus
Schlag, Journal of bacteriology 2008 - “...Berkeley Downregulated genes SA0691 SA1225 SA1226 SA1227 SA1228 SA1229 SA1759 SA2176 SA2183 SA2184 SA2185 SA2186 SA2187 SA2188 SA2189 SA2384 Gene name VOL. 190,...”
- Studies on the mechanism of telavancin decreased susceptibility in a laboratory-derived mutant
Song, Microbial drug resistance (Larchmont, N.Y.) 2013 - “...SAV1330 thrB homoserine kinase homolog SAV1397 dapD tetrahydrodipicolinate acetyltransferase SAV0012 NA putative homoserineo-acetyltransferase Subcategory Fold...”
- Further insights into the mode of action of the lipoglycopeptide telavancin through global gene expression studies
Song, Antimicrobial agents and chemotherapy 2012 - “...Locus ID Amino acid biosynthesis SAV1328 SAV1395 SAV1396 SAV1397 SAV1394 SACOL1364 SAV1329 Song et al. 3162 aac.asm.org bacitracin (48), and the cationic...”
- Transposon sequencing identifies genes impacting Staphylococcus aureus invasion in a human macrophage model
Lo, Infection and immunity 2023 (secret) - In vitro and in vivo models of Staphylococcus aureus endophthalmitis implicate specific nutrients in ocular infection
Sadaka, PloS one 2014 - “...dehydrogenase 14.2 (1.4) SACOL1430 dapA Dihydrodipicolinate synthase 13.5 (1.2) SACOL1431 dapB Dihydrodipicolinate reductase 14.4 (1.2) SACOL1432 dapD 2,3,4,5-tetrahydropyridine-2,6-dicarboxylateN-succinyltransferase 13.0 (1.1) ig_SACOL1432-3 Intergenic region between dapD andSACOL1433 26.4 (1.8) SACOL1433 M20/M25/M40 family peptidase 9.2 (1.3) SACOL1434 Alanine racemase 9.2 (1.2) SACOL1449 sucA 2-oxoglutarate dehydrogenase E1 component 5.2...”
- Investigations into sigmaB-modulated regulatory pathways governing extracellular virulence determinant production in Staphylococcus aureus
Shaw, Journal of bacteriology 2006 - “...same insertion site was found for each of the SACOL1432 (ykuQ), SACOL1433 (hipO), and SACOL1441 (telA) clones, suggesting that these were likely to be siblings....”
- “...of California, Berkeley SACOL0482 SACOL1006 SACOL1428 SACOL1432 SACOL1433 SACOL1435 SACOL1438 SACOL1439 SACOL1441 SACOL1443 SACOL1445 SACOL1947 SACOL2057 a VOL....”
- New Insights into the Biological Functions of Essential TsaB/YeaZ Protein in Staphylococcus aureus
Guo, Antibiotics (Basel, Switzerland) 2024 - “...E5491_RS04085 9 3 down-down thrB E5491_RS06900 6 0 down-down leuC E5491_RS11545 5 0 down-down dapH E5491_RS07270 4 0 down-down tsaB/yeaZ E5491_RS11500 4 groEL E5491_RS11380 4 2 down-down yidC E5491_RS11755 4 13 up-up ilvA E5491_RS11555 4 0 down-down dapB E5491_RS07265 3 0 down-down metE E5491_RS01730 3 3...”
- High-Throughput Mutagenesis Reveals a Role for Antimicrobial Resistance- and Virulence-Associated Mobile Genetic Elements in Staphylococcus aureus Host Adaptation
Ba, Microbiology spectrum 2023 - “...Hypothetical protein S Pig 1384832-1385536 SAPIG2159 prmC Modification methylase hemk family protein J Pig 2235773-2236606 SAPIG1398 dapH_3 2,3,4,5-Tetrahydropyridine-2,6-dicarboxylate N -acetyltransferase E Pig 1472561-1473280 SAPIG1387 pstB Phosphate ABC superfamily ATP-binding cassette transporter, ABC protein P Pig 1460322-1461173 SAPIG0966 DNA segregation ATPase ftsk/spoIIIE D Pig 1013337-1014722 SAPIG1366 Prephenate...”
- Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA
Xu, Frontiers in microbiology 2016 - “...1.91E-02 Dihydrodipicolinate reductase USA300HOU_RS13735 1.82 1.46E-02 Transcriptional regulator USA300HOU_RS08760 rplU 1.79 1.10E-02 Ribosomal protein L21 USA300HOU_RS07115 dapD 1.75 2.17E-02 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase USA300HOU_RS07100 asd 1.74 2.52E-02 Aspartate-semialdehyde dehydrogenase USA300HOU_RS06985 trpB 1.72 4.42E-02 Tryptophan synthase beta subunit USA300HOU_RS06055 pyrE 1.71 2.74E-02 Orotate phosphoribosyltransferase USA300HOU_RS06045 carB 1.68 1.67E-02 Carbamoyl-phosphate...”
rrnAC1106 unknown from Haloarcula marismortui ATCC 43049
52% identity, 18% coverage
- Genome information management and integrated data analysis with HaloLex
Pfeiffer, Archives of microbiology 2008 - “...Shortened HQ1497A, OE2934R rrnAC1042 1,806 1,851 Shortened rrnAC1570, HQ3533A rrnAC1083 1,965 2,010 Shortened NP4322A, OE2871F rrnAC1106 519 420 Extended NP4198A, OE2985F, HQ2561A rrnAC1107 1,476 1,176 Extended NP4904A, HQ1686A rrnAC1115 270 324 Shortened NP4036A, OE2903R, HQ2458A rrnAC1138 849 507 Extended OE2020F, NP1592A rrnAC1169 1,311 867 Extended NP3742A,...”
Dd586_0507 phosphonate metabolim protein, transferase hexapeptide repeat family from Dickeya dadantii Ech586
46% identity, 22% coverage
wbiO / A0A0A8J6G1 acetyl-CoA:α-L-Fuc-(1→2)-β-D-Gal-(1→3)-α-D-GalNAc-(1→3)-α-D-GalNAc-PP-Und 3/4-OIVacetyltransferase from Escherichia coli (see 2 papers)
wzxO127 / A0A0A8J6G1 Escherichia coli O:127 antigen repeat unit flippase from Escherichia coli (see 8 papers)
41% identity, 22% coverage
NWMN_1308 tetrahydrodipicolinate acetyltransferase from Staphylococcus aureus subsp. aureus str. Newman
30% identity, 44% coverage
- Comparative Transcriptome Analysis Reveals Differentially Expressed Genes Related to Antimicrobial Properties of Lysostaphin in Staphylococcus aureus
Yan, Antibiotics (Basel, Switzerland) 2022 - “...Table 2 presents the 15 InDels identified in mutant 1801_2010 compared to Newman_WT. Two genes (NWMN_1308 and NWMN_1410) contained 5 InDels . The remaining 10 InDels were distributed in 5 important intergenic regions: 4 of them were in the intergenic region between the NWMN_0810 and NWMN_0811...”
- “...Insertion Intergenic region (between NWMN_0810 and NWMN_0811) DNAseq mutant reads 1,441,311 ACCC ACC 1 Deletion NWMN_1308 ( dapD ) :tetrahydrodipicolinate acetyltransferase 1,440,676 1,441,395 DNAseq mutant reads 1,441,331 CA CAA 1 Insertion NWMN_1308 ( dapD ) :tetrahydrodipicolinate acetyltransferase 1,440,676 1,441,395 DNAseq mutant reads 1,578,835 GAAA GAA 1...”
- Structure-guided microbial targeting of antistaphylococcal prodrugs
Miller, eLife 2021 - “...PCR Primer TGGGACGAAGTAATTACAGTT Sequence-based reagent NWMN_1192 (pgsA) _R This paper PCR Primer ATATCCCCCTTGTATCGTTT Sequence-based reagent NWMN_1308 (dapD) _F This paper PCR Primer TCTATTCGTGGAGGTACGAT Sequence-based reagent NWMN_1308 (dapD) _R This paper PCR Primer ATCGTATGTGAGCCATTACC Sequence-based reagent NWMN_1410_F This paper PCR Primer CGATAAACCTAAACCACTCG Sequence-based reagent NWMN_1410_R This paper...”
- CodY in Staphylococcus aureus: a regulatory link between metabolism and virulence gene expression
Pohl, Journal of bacteriology 2009 - “...NWMN_1284 NWMN_1304 NWMN_1305 NWMN_1306 NWMN_1307 NWMN_1308 NWMN_1311 NWMN_1348 NWMN_1616 NWMN_1617 NWMN_1749 NWMN_1750 NWMN_1960 NWMN_1961 NWMN_1962 NWMN_1963...”
SAT_STABO / G0YF19 Sophorolipid acetyltransferase; Carboxyhydrate transacetylase; EC 2.3.1.- from Starmerella bombicola (Yeast) (Candida bombicola) (see 4 papers)
46% identity, 21% coverage
- function: Catalyzes the optional acetylation of acidic sophorolipids (SLs) at the 6' and/or 6'' carbon atom of the sophorose head group to produce acetylated acidic SLs in sophorolipid biosynthesis (PubMed:21702032, PubMed:23516968, PubMed:26298016). Has no activity toward lactonic sophorolipids, confirmimg that acetylation must precede lactonization (PubMed:26298016).
disruption phenotype: Produces purely unacetylated sophorolipids. Yields mainly lactonic SLs, in addition to minor amounts of acidic SLs.
VF_A1032 acetyltransferase from Vibrio fischeri ES114
29% identity, 37% coverage
BC4754 putative acetyltransferase/acyltransferase from Bacillus cereus ATCC 14579
38% identity, 28% coverage
WP_000469854 acyltransferase from Escherichia coli
32% identity, 33% coverage
Tmz1t_3784 transferase hexapeptide repeat containing protein from Thauera sp. MZ1T
29% identity, 52% coverage
wbpD / G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-glucuronate N-acetyltransferase monomer (EC 2.3.1.201) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
WBPD_PSEAE / G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-glucuronate N-acetyltransferase (EC 2.3.1.201) from Pseudomonas aeruginosa (see paper)
NP_251846 UDP-2-acetamido-3-amino-2, 3-dideoxy-D-glucuronate N-acetyltransferase from Pseudomonas aeruginosa PAO1
PA3156 probable acetyltransferase WbpD from Pseudomonas aeruginosa PAO1
28% identity, 38% coverage
- function: Plays a role in the biosynthesis of B-band O antigen for serotype O5. Catalyzes the transfer of an acetyl group to the C-3 amino position of UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate (UDP-D-GlcNAc3NA).
catalytic activity: UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + acetyl-CoA = UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + CoA + H(+) (RHEA:33587)
subunit: Homotrimer.
disruption phenotype: Cells lacking this gene are incapable of producing either long-chain B-band O antigen (> or = 2 repeating units) or semi-rough LPS (lipid A-core + one O antigen repeat). - Biosynthesis of UDP-GlcNAc(3NAc)A by WbpB, WbpE, and WbpD: enzymes in the Wbp pathway responsible for O-antigen assembly in Pseudomonas aeruginosa PAO1.
Larkin, Biochemistry 2009 - GeneRIF: Biosynthesis of UDP-GlcNAc(3NAc)A by WbpD enzyme in the Wbp pathway responsible for O-antigen assembly in Pseudomonas aeruginosa PAO1
- Evidence that WbpD is an N-acetyltransferase belonging to the hexapeptide acyltransferase superfamily and an important protein for O-antigen biosynthesis in Pseudomonas aeruginosa PAO1.
Wenzel, Molecular microbiology 2005 (PubMed)- GeneRIF: WbpD belongs to the hexapeptide acyltransferase (HexAT) superfamily of enzymes and involved in the fourth step of biosynthesis of UDP-d-Man(2NAc3NAc)A.
- Genomic Characterization of Arcobacter butzleri Isolated From Shellfish: Novel Insight Into Antibiotic Resistance and Virulence Determinants
Fanelli, Frontiers in microbiology 2019 - “...6V uppP Undecaprenyl-diphosphatase (bacitracin resistance) P60932 Ab 55 Ab 6V wbpD Group B chloramphenicol acetyltransferase G3XD01 Ab 55 Ab 6V The UDP-4-amino-4-deoxy- L -arabinose-oxoglutarate aminotransferase arnB gene was instead identified in both Ab 55 and Ab 6V, while it is not present in A. butlzeri RM4018....”
- Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853
Cao, BMC genomics 2017 - “...LPS biosynthesis protein WbpG PA3153 N/A N/A O-antigen translocase PA3154 N/A N/A B-band O-antigen polymerase PA3156 N/A N/A UDP-2-acetamido-3-amino-2,3-dideoxy-d-glucuronic acid N-acetyltransferase, WbpD PA3157 N/A N/A Probable acetyltransferase, WbpC PA3158 N/A N/A UDP-2-acetamido-2-deoxy-d-glucuronic acid 3-dehydrogenase, WbpB PA3159 N/A N/A UDP-N-acetyl-d-glucosamine 6-Dehydrogenase,WbpA PA3160 ACG06_09160 54.95 O-antigen chain length...”
- Indole and 7-hydroxyindole diminish Pseudomonas aeruginosa virulence
Lee, Microbial biotechnology 2009 - “...wzy 3.5 1.0 1.3 Bband Oantigen polymerase PA3155 wbpE 2.8 1.5 1.2 Probable aminotransferase WbpE PA3156 wbpD 2.3 1.4 1.1 Probable acetyltransferase PA3234 yjcG 4.3 2.0 1.1 Probable sodium:solute symporter PA3337 rfaD 2.0 2.5 1.1 ADP l glycero d mannoheptose 6epimerase PA3450 lsfA 6.1 1.4 1.1...”
- Defining the Pseudomonas aeruginosa SOS response and its role in the global response to the antibiotic ciprofloxacin
Cirz, Journal of bacteriology 2006 - “...PA3145 PA3146 PA3147 PA3148 PA3149 PA3150 PA3153 PA3155 PA3156 PA3157 PA3158 PA3159 PA3160 Fold changea Gene 7106 CIRZ ET AL. J. BACTERIOL. TABLE 1--Continued...”
fdtD / Q12KT8 bifunctional ketoisomerase / N-acetyltransferase FdtD subunit (EC 5.3.2.3; EC 2.3.1.197) from Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) (see paper)
31% identity, 30% coverage
CWATWH0003_3512 acyltransferase from Crocosphaera watsonii WH 0003
28% identity, 42% coverage
fdtC / Q6T1W7 dTDP-3-amino-3,6-dideoxy-α-D-galactopyranose 3-N-acetyltransferase (EC 2.3.1.197) from Aneurinibacillus thermoaerophilus (see 2 papers)
FDTC_ANETH / Q6T1W7 dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose 3-N-acetyltransferase; dTDP-D-Fucp3N acetylase; EC 2.3.1.197 from Aneurinibacillus thermoaerophilus (see paper)
Q6T1W7 dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose 3-N-acetyltransferase (EC 2.3.1.197) from Aneurinibacillus thermoaerophilus (see paper)
28% identity, 52% coverage
- function: Catalyzes the transfer of an acetyl group to dTDP-D-Fucp3N to form dTDP-D-Fucp3NAc in the biosynthesis of dTDP-3-acetamido-3,6- dideoxy-alpha-D-galactose, a glycan chain of the S-layer.
catalytic activity: dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + acetyl-CoA = dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactopyranose + CoA + H(+) (RHEA:32095)
SPD_0949 bacterial transferase hexapeptide (three repeats), putative from Streptococcus pneumoniae D39
32% identity, 42% coverage
- The five homologous CiaR-controlled Ccn sRNAs of Streptococcus pneumoniae modulate Zn-resistance
De, PLoS pathogens 2024 - “...protein 2.16 3.27 SPD_0947 hypothetical protein 2.69 3.97 SPD_0948 nikS nikkomycin biosynthesis protein 3.73 4.29 SPD_0949 N-acetylneuraminate synthase 2.38 4.85 SPD_0950 mefE macrolide ABCE transporter subunit 2.44 3.99 SPD_1045 degenerate DUF3884 domain protein 4.73 6.81 SPD_1046 lacG-2 6-phospho-b-galactosidase 3.56 7.28 SPD_1047 lacE-2 lactose PTS transporter subunit...”
- A Quorum-Sensing System That Regulates Streptococcus pneumoniae Biofilm Formation and Surface Polysaccharide Production
Junges, mSphere 2017 - “...4.87E-03 Membrane protein, putative SPD_0950 ABJ53827.1 17.18 188.70 10.98 5.05E-13 Transporter major facilitator family protein SPD_0949 ABJ54337.1 12.78 100.06 7.83 7.31E-07 Bacterial transferase hexapeptide (three repeats), putative a NCBI protein coding gene. b GenBank. c mRNA log 2 reads. The third and fourth columns show the...”
- “...operon, with no candidate terminators, and the MicrobesOnline database predicts an operon from SPD_0940 to SPD_0949 ( 26 ). While our data cannot exclude the possibility that they actually form a separate operon, it was clear that upon sSHP stimulation, the transcript extended all the way...”
4isxA / Q18A66 The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
45% identity, 20% coverage
- Ligand: acetyl coenzyme *a (4isxA)
VSAL_II0300 hypothetical protein from Vibrio salmonicida LFI1238
VSAL_II0300 acyltransferase from Aliivibrio salmonicida LFI1238
30% identity, 42% coverage
CD0872 maltose O-acetyltransferase from Clostridium difficile 630
45% identity, 20% coverage
KBNAB1_0725 CatB-related O-acetyltransferase from Acinetobacter baumannii
ABAYE0798 chloramphenicol acetyltransferase from Acinetobacter baumannii AYE
43% identity, 18% coverage
- Complete genome of the multidrug-resistant Acinetobacter baumannii strain KBN10P02143 isolated from Korea
Lee, Memorias do Instituto Oswaldo Cruz 2016 - “...streptomycin 3-adenylyltransferase (KBNAB1_1343, KBNAB1_2641), dihydropteroate synthase (KBNAB1_2639 for sulfonamide resistance), dihydrofolate reductase (KBNAB1_3423), chloramphenicol acetyltransferase (KBNAB1_0725, KBNAB1_1342), macrolide 2-phosphotransferase (KBNAB1_1352) and macrolide efflux protein (KBNAB1_1351). The presence of these genes shows that the strain would be capable of overcoming the stimuli induced by diverse antibiotics (...”
- Genomic analysis of Acinetobacter baumannii A118 by comparison of optical maps: identification of structures related to its susceptibility phenotype
Ramirez, Antimicrobial agents and chemotherapy 2011 - “...other A. baumannii strains (strain AYE, locus tag ABAYE0798; strain AB0057, locus tag AB57_3104; strain ATCC 17978, locus tag A1S_2691) (Fig. 2b). These results...”
- “...acetyltransferase amino acid sequences. Strain AYE, locus tag ABAYE0798; strain AB0057, locus tag AB57_3104; strain ATCC 17978, locus tag A1S_2691. VOL. 55,...”
RL0815 putative O-acetyl transferase from Rhizobium leguminosarum bv. viciae 3841
53% identity, 18% coverage
- Rhizobium determinants of rhizosphere persistence and root colonization
Knights, The ISME journal 2024 - “...of the Gram-negative outer membrane. Six enzymes of a LPS biosynthesis cluster, encoded by RL0813, RL0815, RL0818, RL0822, gmd (RL0825), and fcl (RL0826), were essential for colonization across plant species. Other LPS biosynthesis enzymes important for root colonization include a glycosyl transferase (RL1470), an LPS assembly...”
SAR2635 putative acetyltransferase from Staphylococcus aureus subsp. aureus MRSA252
25% identity, 44% coverage
SPAC18B11.09c N-acetyltransferase (predicted) from Schizosaccharomyces pombe
41% identity, 21% coverage
- A single m6A modification in U6 snRNA diversifies exon sequence at the 5' splice site
Ishigami, Nature communications 2021 - “...of the Z -score of each intron. The red and blue arrows represent plots of SPAC18B11.09c intron #1 and sec71 intron #1, respectively. The black line represents an equal value of IRS. c Semi-quantitative RT-PCR analyses of two introns with large IRS difference. The upper and...”
- “...equal value of IRS. b Mutation study with a minigene construct encoding intron #1 of SPAC18B11.09c . Splicing efficiency was measured by RT-PCR using minigene-specific primers, followed by non-denaturing PAGE analysis. The IRS was quantified from the signal intensity ratio of the PCR products with and...”
MUL_4655 serine acetyltransferase CysE_1 from Mycobacterium ulcerans Agy99
34% identity, 36% coverage
D2S363 Transferase hexapeptide repeat containing protein from Haloterrigena turkmenica (strain ATCC 51198 / DSM 5511 / JCM 9101 / NCIMB 13204 / VKM B-1734 / 4k)
42% identity, 22% coverage
EPSM_BACSU / P71063 UDP-N-acetylbacillosamine N-acetyltransferase; EC 2.3.1.203 from Bacillus subtilis (strain 168) (see 3 papers)
30% identity, 41% coverage
- function: Catalyzes the conversion of UDP-2,4,6-trideoxy-2-acetamido-4- amino glucose to UDP-2,4,6-trideoxy-2,4-diacetamido glucose, commonly known as UDP-N,N'-diacetylbacillosamine (UDP-diNAcBac).
catalytic activity: UDP-N-acetylbacillosamine + acetyl-CoA = UDP-N,N'- diacetylbacillosamine + CoA + H(+) (RHEA:34159)
subunit: Forms oligomers.
disruption phenotype: Insertion mutant shows decreased efficiency of biofilm formation. Disruption affects swarming motility. - EpsM from Bacillus subtilis 168 has UDP-2,4,6-trideoxy-2-acetamido-4-amino glucose acetyltransferase activity in vitro
Kaundinya, Biochemical and biophysical research communications 2018 (PubMed)- “...V. Abstract Bacillus subtilis 168 EpsM (UniProt id P71063) has been electronically annotated as putative acetyltransferase in the UniProt database. The gene...”
- “...Abstract Bacillus subtilis 168 EpsM (UniProt id P71063) has been electronically annotated as putative acetyltransferase in the UniProt database. The gene...”
A1S_0054 WbbJ protein from Acinetobacter baumannii ATCC 17978
26% identity, 42% coverage
PF0768 acetyl / acyl transferase related protein from Pyrococcus furiosus DSM 3638
27% identity, 47% coverage
SSUR61_2113 DapH/DapD/GlmU-related protein from Streptococcus suis R61
30% identity, 40% coverage
AFUA_3G03380, Afu3g03380 sugar O-acetyltransferase, putative from Aspergillus fumigatus Af293
42% identity, 21% coverage
- Regulation of Secondary Metabolism by the Velvet Complex Is Temperature-Responsive in Aspergillus
Lind, G3 (Bethesda, Md.) 2016 - “..., Afu3g03310 , Afu3g03315 , Afu3g03320 , Afu3g03330 , Afu3g03340 , Afu3g03350 , Afu3g03370 , Afu3g03380 , Afu3g03390 , Afu3g03400 , Afu3g03410 , Afu3g03420 , Afu3g03430 , Afu3g03440 , Afu3g03445 , Afu3g03450 , Afu3g03460 Inglis et al. (2013) Cluster 13 Hexadehydroastechrome (HAS) cluster Afu3g12890 , Afu3g12900...”
- Regulation of sulphur assimilation is essential for virulence and affects iron homeostasis of the human-pathogenic mould Aspergillus fumigatus
Amich, PLoS pathogens 2013 - “...AFUA_7G06140 - D -glucoside glucohydrolase 26.38 2,2E-17 AFUA_7G06130 plasma membrane protein Pth11-like, putative 23.16 3,1E-27 AFUA_3G03380 sugar O-acetyltransferase, putative 17,14 3,6E-50 AFUA_3G03390 * siderophore biosynthesis lipase/esterase 14.89 4,6E-50 AFUA_2G07680 * L -ornithine N 5 -oxygenase SidA (SM) 14.22 2,2E-74 AFUA_3G07640 plasma membrane H + -ATPase 14.01...”
- Sub-telomere directed gene expression during initiation of invasive aspergillosis
McDonagh, PLoS pathogens 2008 - “...Afu2g10910 maltose permease 0.55716766 Afu6g13230 Nit protein 2 2.6084555 Afu1g03280 maltose permease Secreted Proteins 3.2031907 Afu3g03380 maltose O-acetyltransferase 7.40411592 Afu5g14190 beta-glucanase 2.5614034 Afu8g07070 maltase 5.898216645 Afu1g17510 lipase/esterase 4.678081 Afu7g06380 maltase 5.644563577 Afu2g09380 cutinase 3.4802952 Afu4g00150 MFS maltose transporter 5.496020587 Afu8g07090 extracellular proline-serine rich protein 2.2425193 Afu8g07240...”
CAC2381 Tetrahydrodipicolinate N-succinyltransferase from Clostridium acetobutylicum ATCC 824
31% identity, 45% coverage
- Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis
Senger, Biotechnology and bioengineering 2008 - “...CAC3171, CAC1479. List 3: CAC3169/CAC3176/ CAC3652, CAC0091, CAC3170/CAC3604, CAC1479, CAC3038. List 4: CAC0998, CAC2378/CAC3600, CAC2379, CAC2381, CAC2380, CAC2723, CAC2624, CAC0608. List 5: CAC1825, CAC0390, CAC0391, CAC3348/CAC0578. List 6: CAC0737/CAC1001/CAC1819/CAC2832/CAC0764/CAC1673/CAC1674. List 7: (from DAHP) CAC0894, CAC0713/CAC0899, CAC0897, CAC0898, CAC0895, CAC0896. List 8: CAC2680, CAC0944, CAC1348, CAC1730, CAC0726,...”
C5O78_00405 acyltransferase from Treponema phagedenis
29% identity, 41% coverage
9jywA / A0A223E5F5 Crystal structure of the gamma-carbonic anhydrase from the polyextremophilic bacterium aeribacillus pallidus
34% identity, 33% coverage
CCAN12_760043 acetyltransferase from Capnocytophaga canimorsus
30% identity, 35% coverage
EF2957 hexapeptide-repeat containing-acetyltransferase from Enterococcus faecalis V583
27% identity, 47% coverage
BHE19_RS15305 acetyltransferase from Flavobacterium tructae
25% identity, 60% coverage
BL03105 DapH/DapD/GlmU-related protein from Bacillus licheniformis DSM 13 = ATCC 14580
45% identity, 18% coverage
SF5M90T_1724 CatB-related O-acetyltransferase from Shigella flexneri 5a str. M90T
SF1773 putative acetyltransferase from Shigella flexneri 2a str. 301
46% identity, 19% coverage
- RNA-seq analysis of the influence of anaerobiosis and FNR on Shigella flexneri
Vergara-Irigaray, BMC genomics 2014 - “...-2.60 SF5M90T_275 putative crossover junction endodeoxyribonuclease 2.84 SF5M90T_2418 muQ N-acetylmuramic acid 6-phosphate etherase 2.77 -0.93 SF5M90T_1724 putative acetyltransferase 2.06 -2.41 SF5M90T_2435 putative amino acid antiporter 2.03 1.90 1.58 SF5M90T_2301 yfbT putative phosphatase 2.00 SF5M90T_773 ybhG putative membrane protein 1.78 1.02 SF5M90T_1227 hns DNA-binding protein 1.69 -1.24...”
- Virulence and Stress Responses of Shigella flexneri Regulated by PhoP/PhoQ
Lin, Frontiers in microbiology 2017 - “...tctgc GGTTTt t Hypothetical protein ubiC Chromosome a GGTTcA acagc GtTTTA c Chorismate pyruvate lyase SF1773 Chromosome g aGTTTA atggc GGTTaA g Acetyltransferase yjjM Chromosome t GtTTTA aatcg GGTTTt a Hypothetical protein lpdA Chromosome t TGTTTA aaaat TGTTaA c Dihydrolipoamide dehydrogenase cbpA Chromosome c TGTTTA aaata...”
KP1_3712 galactoside O-acetyltransferase from Klebsiella pneumoniae NTUH-K2044
45% identity, 16% coverage
HVO_1658 acetyltransferase homolog from Haloferax volcanii DS2
47% identity, 19% coverage
Clocel_1978 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase from Clostridium cellulovorans 743B
27% identity, 48% coverage
- Clostridium cellulovorans Proteomic Responses to Butanol Stress
Costa, Frontiers in microbiology 2021 - “...biosynthesis of a number of amino acids including proline and arginine (Clocel_2734, Clocel_3150, Clocel_1668), lysine (Clocel_1978, Clocel_3115), methionine (Clocel_1764, Clocel_2896, Clocel_3040), and branched chain amino acids (BCAA) (Clocel_1324, Clocel_1325, Clocel_0493) ( Table 1 and Supplementary Table 2 ). In addition, up-regulated proteins include four aminotransferases (Clocel_1948,...”
BL1719 probable sugar O-acetyltransferase (thiogalactoside acetyltransferase from Bifidobacterium longum NCC2705
45% identity, 17% coverage
- Investigation of Effects of Novel <i>Bifidobacterium longum</i> ssp. <i>longum</i> on Gastrointestinal Microbiota and Blood Serum Parameters in a Conventional Mouse Model
Rätsep, Microorganisms 2024 - “...7 ]. Recently, we have reported a novel Bifidobacterium longum ssp. longum strain BIOCC 1719 (BL1719) of healthy child origin [ 11 ]. We characterised the metabolic diversity of the strain by determining bioactive compounds like B vitamins, essential amino acids, and fatty acids that the...”
- “...can enrich human nutrition. The aim of this study was to assess the biosafety of BL1719 on a mouse model and to elucidate its potential impact on the gut microbiota and the metabolic profile of the host when administered in live form at a specific dosage....”
- A Novel <i>Bifidobacterium longum</i> ssp. <i>longum</i> Strain with Pleiotropic Effects
Rätsep, Microorganisms 2024 - “...2.1. Origin of the Strain The microorganism Bifidobacterium longum strain BIOCC 1719 (DSM 34239) (hereinafter BL1719) was isolated from a stool sample of a 2-month-old full-term breastfed Estonian child in 2020. The strain was identified by MALDI-TOF MS (Bruker MicroFlex Biotyper, Billerica, MA, USA) as Bifidobacterium...”
- “...analysis. The strain was characterised regarding safety and functional properties (patent pending). 2.2. Screening of BL1719 Metabolites in Different Growth Media 2.2.1. Growth Media and Cultivation Conditions A suspension from the 24 h old BL1719 culture was inoculated in a final inoculation dose of 5.5 log...”
A9IH93 UDP-2-acetamido-3-amino-2,3-dideoxy-glucuronate N-acetyltransferase (EC 2.3.1.201) from Bordetella petrii (see paper)
24% identity, 52% coverage
YJL218W Putative protein of unknown function, similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene from Saccharomyces cerevisiae
P40892 Putative acetyltransferase YJL218W from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
29% identity, 39% coverage
- Exploring Saccharomycotina Yeast Ecology Through an Ecological Ontology Framework
Harrison, Yeast (Chichester, England) 2024 - “...is annotated in the S. cerevisiae genome as an uncharacterized ORF with the systematic name YJL218W. In yeasts isolated from animals, 87% (295/339) have a copy of this gene compared to only 66% (495/747) of nonanimal yeasts. Previous work has shown that Oaf1p/Pip2p induces this gene...”
- “...of oleate as a singular carBon source ( Rottensteiner et al. 2003 ). Moreover, the YJL218W deletion strain of S. cerevisiae had decreased cell membrane integrity and reduced capacity to grow in high salt concentrations ( Li et al. 2022 ). In a general framework, the...”
- Systematic profiling of ale yeast protein dynamics across fermentation and repitching
Garge, G3 (Bethesda, Md.) 2024 - “...and YKL091C , YLR177W , YDR391C , YER034W , YKL063C , YER152C , YML020W , YJL218W , YNL011C , YGR266W , YBR137W , YBL055C in cluster 7c from Fig. 2 , see Supplementary Table 7 ). Further studies characterizing these sets of proteins will shed light...”
- Deletion of YJL218W reduces salt tolerance of Saccharomyces cerevisiae
Li, Journal of basic microbiology 2022 (PubMed)- “...The YJL218W open reading frame may be involved in peroxisomal biogenesis. However, whether it mediates salt tolerance is unclear. We found that after knockdown of YJL218W in Saccharomyces cerevisiae (S. cerevisiae), its...”
- “...tolerance was reduced and cell death was increased. Transcriptome sequencing and analysis further revealed that YJL218W knockdown mediated significant changes in the expression of 1432 messenger RNA (mRNAs), of which 603 were upregulated. KEGG enrichment analysis and polymerase chain reaction (PCR) assay indicated that YJL218W mediated...”
- Complete genome sequence and analysis of a Saccharomyces cerevisiae strain used for sugarcane spirit production
Costa, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 2021 - “...(MST28) YAR027W (UIP3) YAR031W (PRM9) YGL053W (PRM8) YJL218W YJL217W (REE1) YLR155C (ASP3-1) YLR157C (ASP3-2) YLR157C (ASP3-3) YLR160C (ASP3-4) YGR288W (MAL13)...”
- Identification of the Genetic Requirements for Zinc Tolerance and Toxicity in Saccharomyces cerevisiae
Zhao, G3 (Bethesda, Md.) 2020 - “...YHR112C YKL044W MMO1 YNL204C SPS18 YDL041W YHR151C MTC6 YLR149C YPL261C YDR203W YJL211C YLR232W YPR123C YDR417C YJL218W YMR265C a Gene names were listed alphabetically according to their systemic names. b The mutations for 48 genes that were reported sensitive to zinc previously were underlined. Gene Ontology (GO)...”
- Transcriptome Profile of Yeast Strain Used for Biological Wine Aging Revealed Dynamic Changes of Gene Expression in Course of Flor Development
Mardanov, Frontiers in microbiology 2020 - “...(Dn4) at the mature biofilm stage as compared to the early stage. Five genes, namely YJL218W (acetyltransferase), AIF1 (apoptosis-inducing factor 1), ENB1 (siderophore iron transporter), REE1 (regulator of enolase expression), CSS3 (hypothetical protein) were found to be expressed only at the mature biofilm stage. FIGURE 1...”
- The Swr1 chromatin-remodeling complex prevents genome instability induced by replication fork progression defects
Srivatsan, Nature communications 2018 - “...htz1 mutant, none of these genes showed increased expression, and two GCR-suppressing genes, PHR1 and YJL218W , showed a minor ~40% reduction in expression. Defects in PHR1 and YJL218W result in a minor increase in GCR patch score in the sGCR assay, which is of borderline...”
- Pathways and Mechanisms that Prevent Genome Instability in Saccharomyces cerevisiae
Putnam, Genetics 2017 - “...KCH1 YJL173C RFA3 YDL162C YDL162C YDR499W LCD1 YJR068W RFC2 YHL026C YHL026C YDR439W LRS4 YOL094C RFC4 YJL218W YJL218W YBL023C MCM2 YBR087W RFC5 YKR023W YKR023W YLR274W MCM5 YLR453C RIF2 YMR284W YKU70 YBR136W MEC1 YPR018W RLF2 YMR106C YKU80 YLR288C MEC3 YDR255C RMD5 YML002W YML002W YIL128W MET18 YPL024W RMI1 YML020W...”
- More
- Regulation and adaptive evolution of lactose operon expression in Lactobacillus delbrueckii.
Lapierre, Journal of bacteriology 2002
ABLAC_08080 CatB-related O-acetyltransferase from Acinetobacter baumannii LAC-4
41% identity, 18% coverage
XCV2008 putative acetyltransferase from Xanthomonas campestris pv. vesicatoria str. 85-10
37% identity, 36% coverage
3mqgC / A9IH93 Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with acetyl-coa (see paper)
24% identity, 52% coverage
- Ligands: uridine-5'-monophosphate; acetyl coenzyme *a; uridine-5'-diphosphate (3mqgC)
NP_246134 VatB from Pasteurella multocida subsp. multocida str. Pm70
47% identity, 17% coverage
CAG_GEOKA / Q5KW03 Carbonic anhydrase; Gamma-carbonic anhydrase; Cag; EC 4.2.1.1 from Geobacillus kaustophilus (strain HTA426) (see 2 papers)
GK2848 hypothetical protein from Geobacillus kaustophilus HTA426
39% identity, 30% coverage
- function: Reversible hydration of carbon dioxide.
catalytic activity: hydrogencarbonate + H(+) = CO2 + H2O (RHEA:10748)
cofactor: Mg(2+) Zn(2+) (Either Mg(2+) or Zn(2+) can occupy the active site.)
subunit: Homotrimer. - Structural and functional characterization of a putative carbonic anhydrase from Geobacillus kaustophilus reveals its cambialistic function
Sridharan, Biochemical and biophysical research communications 2021 (PubMed)- “...structural and functional analysis of a hypothetical protein GK2848 from Geobacillus kaustophilus. The analysis revealed that it belongs to the -class of CA...”
- “...authors thank C. Kuroishi for cloning of the gk2848 gene. This work was supported by the RIKEN Structural Genomic/Proteomics Initiative (RSGI) , the National...”
- Structural Organization of Enzymes of the Phenylacetate Catabolic Hybrid Pathway
Grishin, Biology 2015 - “...Enoyl-CoA hydratase; Crotonase superfamily PaaH 3-hydroxyadipyl-CoA dehydrogenase 3MOG E. coli 3-hydroxyacyl-CoA dehydrogenase PaaY 2-hydroxycyclohepta-1,4,6-triene-1-carboxyl-CoA thioesterase GK2848 3VNP Geobacillus kaustophilus PaaX Transcriptional repressor PaaX-like protein 3LO9 Jannaschia sp. PaaX-like proteins containing helix-turn-helix motif All chemical reactions within this pathway have been defined, intermediates identified and enzyme functions...”
- Crystallization, characterization and preliminary X-ray crystallographic analysis of GK2848, a putative carbonic anhydrase of Geobacillus kaustophilus
Ragunathan, Acta crystallographica. Section F, Structural biology and crystallization communications 2013 - “...and preliminary X-ray crystallographic analysis of GK2848, a putative carbonic anhydrase of Geobacillus kaustophilus GK2848, a hypothetical protein...”
- “...and belonged to the orthorhombic space group P21212. GK2848 bears sequence homology to carbonic anhydrases of various bacterial species, indicating that it...”
SAG0913 chloramphenicol acetyltransferase from Streptococcus agalactiae 2603V/R
43% identity, 18% coverage
A1S_2691 antibiotic acetyltransferase from Acinetobacter baumannii ATCC 17978
41% identity, 18% coverage
YP_009329047 Hexapeptide transferase from Cedratvirus A11
44% identity, 18% coverage
- The Viral Fraction Metatranscriptomes of Lake Baikal
Potapov, Microorganisms 2022 - “...26 Macrocin O-methyltransferase YP_009329463 6.4 10 25 PD-(D/E)XK nuclease YP_009329328 2.5 10 17 Hexapeptide transferase YP_009329047 4.9 10 12 5nucleotidase/apyrase YP_009329013 8.0 10 18 Putative serine/threonine-protein kinase/receptor YP_009329205 9.7 10 10 microorganisms-10-01937-t002_Table 2 Table 2 AMG genes identified in metatranscriptomes of Lake Baikal. AMG KO Name...”
MGAS2096_Spy1118 Virginiamycin A acetyltransferase from Streptococcus pyogenes MGAS2096
44% identity, 19% coverage
3vnpA / Q5KW03 Crystal structure of hypothetical protein (gk2848) from geobacillus kaustophilus
39% identity, 30% coverage
- Ligand: magnesium ion (3vnpA)
PGN_0913 putative acetyltransferase from Porphyromonas gingivalis ATCC 33277
34% identity, 38% coverage
- Post-translational regulation of a Porphyromonas gingivalis regulator
Li, Journal of oral microbiology 2018 - “...these, six were annotated as follows: a probable serine acetyltransferase (PGN_0230); a metal binding acetyltransferase (PGN_0913); an alginate O-acetyltransferase (PGN_0943); a lipid phospholipid acetyltransferase (PGN_1142); a probable 1-acyl-sn-glycerol 3-phosphate acetyltransferase (PGN_1384); and a lysophospholipid acetyltransferase (PGN_2086). The remaining three (PGN_0932, PGN_1389, PGN_1729) were annotated as GNAT...”
PG1211 hexapeptide transferase family protein from Porphyromonas gingivalis W83
33% identity, 38% coverage
- Metabolome variations in the Porphyromonas gingivalis vimA mutant during hydrogen peroxide-induced oxidative stress
McKenzie, Molecular oral microbiology 2015 - “...PG1145 2.0970461 Long-chain-fatty-acidCoA ligase, putative PG1201 12.248168 Conserved hypothetical protein, degenerate PG1202 2.0986403 Hypothetical protein PG1211 2.0124948 Hexapeptide transferase family protein PG1233 5.4165149 Hypothetical protein PG1235 2.0797322 Epimerase/reductase, putative PG1236 5.5059223 Hypothetical protein PG1294 2.0615625 Ferrous iron transport protein B PG1300 2.082116 Conserved hypothetical protein PG1305...”
- “...PG0199 TatD family protein PG0721 NLP/P60 family protein PG1124 DUF 80 domain protein (exosortase like) PG1211 Hexa peptide transferase PG1383 Amino acid exporter PG0562 Potassium uptake/transport protein-Trk PG0134 Magnesium transporter protein PG2164 Peptidyl polycis-trans isomerase PG0962 Prolyl trna synthetase PG1583 Auto transporter BatB Upregulated 15 min...”
YP_001555452 WxcM-like protein from Shewanella baltica OS195
32% identity, 30% coverage
- Implication of lateral genetic transfer in the emergence of Aeromonas hydrophila isolates of epidemic outbreaks in channel catfish
Hossain, PloS one 2013 - “...fdtB Shewanella baltica OS195 81 YP_001555451.1 ORF22 24796.25233 dTDP-D-Fucp3N acetyltransferase fdtC Shewanella baltica OS195 85 YP_001555452.1 ORF23 25235.25657 dTDP-6-deoxy-3,4-keto-hexulose isomerase fdtA Shewanella putrefaciens 200 72 ADV52549.1 ORF24 25668.26534 glucose-1-phosphate thymidylyltransferase rmlA Shewanella putrefaciens 200 79 NP_718742 ORF25 26531.27619 dTDP-glucose-4-6-dehydratase rmlB Aeromonas hydrophila 93 AAM22544.1 ORFd 28234.31383...”
- “...fdtB Shewanella baltica OS195 81 YP_001555451 ORF22 24796.25233 dTDP-D-Fucp3N acetyltransferase fdtC Shewanella baltica OS195 85 YP_001555452 ORF23 25235.25657 dTDP-6-deoxy-3,4-keto-hexulose isomerase fdtA Shewanella putrefaciens 200 72 ADV52549 ORF24 25668.26534 glucose-1-phosphate thymidylyltransferase rmlA Shewanella putrefaciens 200 79 ADV52548 ORF25 26531.27619 dTDP-glucose-4-6-dehydratase rmlB Aeromonas hydrophila 93 AAM22544 ORFd 28234.31383...”
CNAG_05023 maltose O-acetyltransferase from Cryptococcus neoformans var. grubii H99
27% identity, 43% coverage
- HDAC genes play distinct and redundant roles in Cryptococcus neoformans virulence
Brandão, Scientific reports 2018 - “...0.583727866 CNAG_03929 CAS42 Solute carrier family 35, member C2 0.140514137 CNAG_04312 MAN1 Mannose-6-phosphate isomerase 0.199177397 CNAG_05023 CAS91 Putative maltose o-acetyltransferase 0.396029552 CNAG_05081 PDE1 Phosphodiesterase 0.17141564 CNAG_05139 UGT1 Solute carrier family 35 (UDP-sugar transporter), member A1/2/3 0.135099801 CNAG_05144 CAN2 Carbonic anhydrase 0.881416777 CNAG_05222 NRG1 Transcriptional regulator Nrg1...”
LQ939_RS12565 DapH/DapD/GlmU-related protein from Pantoea alhagi
28% identity, 37% coverage
GLRG_01112 acetyltransferase from Colletotrichum graminicola M1.001
26% identity, 40% coverage
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 789,361 different protein sequences to 1,256,019 scientific articles. Searches against EuropePMC were last performed on January 10 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory