PaperBLAST
PaperBLAST Hits for BRENDA::Q31RQ5 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (Synechococcus elongatus) (448 a.a., MAQPFVLLPT...)
Show query sequence
>BRENDA::Q31RQ5 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (Synechococcus elongatus)
MAQPFVLLPTEDSHQTLRIQPPSTGIGLRGRIRVPGDKSISHRALMLGAIASGETTIEGL
LLGEDPLSTAACFRAMGAEISELNSELVRVKGIGLQNLQEPLDVLNAGNSGTTIRLMMGL
LAGQRDRFFCVTGDESLRSRPMARVIQPLSQMGAEIRGRQGNTRAPLAISGRSLQPIRYV
SPIASAQVKSSILLAGLTCEGQTTVVEPALSRDHSERMFRAFGAKLTVNPEEISVTVEGP
AELTGQPVVVPGDISSAAFWLVAAAIVPDSDLLIENVGVNPTRTGILEALQQMEAQITLE
NERIVAGEPVADLRVRSSNLQAIEIGGSLIPRLIDEVPILAVAAAFAKGTTIIRDAEELR
VKESDRIAVMASELGRMGATISERPDGLEITGGAALTGATVDSYTDHRIAMSLAIAALQA
KGQTQIQQAEAAAVSYPDFVPTLQQLLG
Running BLASTp...
Found 216 similar proteins in the literature:
Q31RQ5 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Synechococcus elongatus (see paper)
Q5N2J9 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Synechococcus elongatus (see paper)
100% identity, 100% coverage
A0A139XBW5 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Scytonema hofmannii (see paper)
75% identity, 99% coverage
K9TX81 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Chroococcidiopsis thermalis (see paper)
74% identity, 100% coverage
K9QNN0 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Nostoc sp. (see paper)
72% identity, 100% coverage
A0A0P4UM05 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Leptolyngbya boryana (see paper)
73% identity, 98% coverage
A0A0D6KU47 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Tolypothrix sp. (see paper)
74% identity, 97% coverage
B1XM36 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Synechococcus sp. (see paper)
72% identity, 99% coverage
I4GHG6 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Microcystis aeruginosa (see paper)
72% identity, 97% coverage
K9VBD9 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Oscillatoria sp. (see paper)
73% identity, 96% coverage
B4VHX1 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Coleofasciculus chthonoplastes (see paper)
73% identity, 95% coverage
K9RIB8 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Rivularia sp. PCC 7116 (see paper)
72% identity, 93% coverage
K9TRT1 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Oscillatoria sp. (see paper)
71% identity, 97% coverage
slr0444 3-phosphoshikimate 1-carboxyvinyltransferase from Synechocystis sp. PCC 6803
72% identity, 98% coverage
- Proteomic analysis reveals resistance mechanism against biofuel hexane in Synechocystis sp. PCC 6803
Liu, Biotechnology for biofuels 2012 - “...17.3 kd protein mura rpon intergenic region precursor Sll1804 1.55 30S ribo somal protein S3 Slr0444 1.54 3-phosphoshikimate 1-carboxyvinyltransterase Sll1821 1.80 50S ribosomal protein L13 Sll1813 1.60 50S ribosomal protein L15 Ssl3436 2.58 2.06 1.72 1.71 50S ribosomal protein L29 Ssl1426 2.07 50S ribosomal protein L35...”
- Gene expression patterns of sulfur starvation in Synechocystis sp. PCC 6803
Zhang, BMC genomics 2008 - “...(ssr1480, ssl3441 ( infA ), Ribosomal protein ssr2799 ( rpl27 )) 15 sll1184( ho1 ), slr0444( aroA ), slr1176( agp ), sll0807( cfxE ), slr1545( rpoE ), Cytochrome b 6/f complex subunit smr0003( petM ), Photosystem I (smr0005( psaM ), ssl0563( psaC )), Photosystem II (sll1867(...”
B4WHQ7 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Synechococcus sp. (see paper)
68% identity, 89% coverage
F5A7Q0 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Bacillus cereus (see paper)
55% identity, 96% coverage
JS609_02206 3-phosphoshikimate 1-carboxyvinyltransferase from Bacillus subtilis
51% identity, 94% coverage
aroE / P20691 5-enolpyruvoylshikimate-3-phosphate synthase (EC 2.5.1.19) from Bacillus subtilis (strain 168) (see 3 papers)
BSU22600 3-phosphoshikimate 1-carboxyvinyltransferase from Bacillus subtilis subsp. subtilis str. 168
P20691 3-phosphoshikimate 1-carboxyvinyltransferase from Bacillus subtilis (strain 168)
51% identity, 94% coverage
- The Blueprint of a Minimal Cell: MiniBacillus
Reuß, Microbiology and molecular biology reviews : MMBR 2016 - “...monocytogenes aroB aroC aroD aroE BSU22700 BSU23080 BSU25660 BSU22600 No No No No 4.2.3.4 4.2.1.10 1.1.1.25 2.5.1.19 3CLH 1QFE 2EGG 3RMT Helicobacter pylori S....”
- First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...were obtained from: Bacillus subtilis (P20691), Streptococcus pneumoniae (Q9S400), Staphylococcus aureus (Q05615), Dichelobacter nodosus (Q46550), Halovibrio...”
- A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity
Peng, PloS one 2012 - “...proteins were obtained from Pseudomonas strain PG2982 (P56952), A. tumefaciens strain CP4 (Q9R4E4), Bacillus subtilis (P20691), S. aureus (Q05615), and S. pneumoniae (Q9S400). Growth of cells in the presence of glyphosate The growth curves of the E. coli aroA-deleted mutant ER2799 harboring plasmid p251-AroA-Ra or p251-AroA-Ec...”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...(Swiss-Prot Q9R4E4), Bacillus subtilis (Swiss-Prot P20691), Staphylococcus aureus (Swiss-Prot Q05615), Dichelobacter nodosus (Swiss-Prot Q46550), and...”
- Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Sun, Applied and environmental microbiology 2005 - “...tumefaciens CP4 (Q9R4E4), Bacillus subtilis (P20691), Staphylococcus aureus (Q05615), Dichelobacter nodosus (Q46550), and Streptococcus pneumoniae (Q9S400)....”
AROA1_HALH5 / Q9KCA6 3-phosphoshikimate 1-carboxyvinyltransferase 1; 5-enolpyruvylshikimate-3-phosphate synthase 1; EPSP synthase 1; EPSPS 1; EC 2.5.1.19 from Halalkalibacterium halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) (Bacillus halodurans)
55% identity, 94% coverage
- function: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256)
subunit: Monomer.
JS608_02738, JTE87_01546 3-phosphoshikimate 1-carboxyvinyltransferase from Bacillus amyloliquefaciens
52% identity, 94% coverage
- Multi-Omics Characterization of Host-Derived Bacillus spp. Probiotics for Improved Growth Performance in Poultry
Susanti, Frontiers in microbiology 2021 - “...PTA-86 Protein AroA(G) aro A 5.4.99.5 JS608_03349 JTE87_00934 JS609_02973 3-phosphoshikimate 1-carboxyvinyltransferase 1 aro A1 2.5.1.19 JS608_02738 JTE87_01546 JS609_02206 3-dehydroquinate synthase aro B 4.2.3.4 JS608_02748 JTE87_01536 JS609_02216 Chorismate synthase aro C 4.2.3.5 JS608_02749 JTE87_01535 JS609_02217 3-dehydroquinate dehydratase aro D 4.2.1.10 JS608_01242 JTE87_03044 JS609_02255 Shikimate dehydrogenase [NADP(+)] aro...”
- “...Protein AroA(G) aro A 5.4.99.5 JS608_03349 JTE87_00934 JS609_02973 3-phosphoshikimate 1-carboxyvinyltransferase 1 aro A1 2.5.1.19 JS608_02738 JTE87_01546 JS609_02206 3-dehydroquinate synthase aro B 4.2.3.4 JS608_02748 JTE87_01536 JS609_02216 Chorismate synthase aro C 4.2.3.5 JS608_02749 JTE87_01535 JS609_02217 3-dehydroquinate dehydratase aro D 4.2.1.10 JS608_01242 JTE87_03044 JS609_02255 Shikimate dehydrogenase [NADP(+)] aro E...”
A7Z612 3-phosphoshikimate 1-carboxyvinyltransferase from Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / LMG 26770 / FZB42)
52% identity, 94% coverage
K4120_10745 3-phosphoshikimate 1-carboxyvinyltransferase from Bacillus velezensis
52% identity, 94% coverage
- Production-optimized fermentation of antifungal compounds by bacillus velezensis LZN01 and transcriptome analysis
Hu, Microbial biotechnology 2024 - “...optimization group, including K4120_13830 (bifunctional 3deoxy7phosphoheptulonate synthase/chorismate mutase), K4120_01665 (shikimate kinase), K4120_04005 (shikimate dehydrogenase) and K4120_10745 (3phosphoshikimate 1carboxyvinyltransferase); serA (phosphoglycerate dehydrogenase), serC (3phosphoserine/phosphohydroxythreonine transaminase), K4120_07650 and K4120_01805 (pyridoxal phosphatedependent aminotransferase), K4120_15570 , K4120_15845 , K4120_13595 and K4120_17885 (SDR family oxidoreductase). However, the expression levels of most...”
EF1566 3-phosphoshikimate 1-carboxyvinyltransferase from Enterococcus faecalis V583
51% identity, 96% coverage
- Transcriptome, proteome, and metabolite analyses of a lactate dehydrogenase-negative mutant of Enterococcus faecalis V583
Mehmeti, Applied and environmental microbiology 2011 - “...to be downregulated significantly (EF1562, EF1564, EF1565, and EF1566). Among the three plasmids of the V583 strain, only pTEF2 carries genes that were...”
- Class IIa bacteriocin resistance in Enterococcus faecalis V583: the mannose PTS operon mediates global transcriptional responses
Opsata, BMC microbiology 2010 - “...diphosphate kinase EF1227 -0.01 1.06 conserved hypothetical protein EF1422 0.11 0.85 transcriptional regulator, Cro/CI family EF1566 -0.64 0.57 3-phosphoshikimate 1-carboxyvinyltransferase ( aroA ) EF1567 -0.39 0.52 shikimate kinase ( aroK ) EF1603 -0.15 1.01 sucrose-6-phosphate dehydrogenase ( scrB-1 ) EF1619 -0.33 2.31 carbon dioxide concentrating mechanism...”
- Comparison of OG1RF and an isogenic fsrB deletion mutant by transcriptional analysis: the Fsr system of Enterococcus faecalis is more than the activator of gelatinase and serine protease
Bourgogne, Journal of bacteriology 2006 - “...biosynthesis EF1561 EF1562 EF1563 EF1564 EF1565 EF1566 EF1567 EF1568 EF1569 aroE P-2-dehydro-3-deoxyheptonate aldolas aroB aroC Prephenate dehydrogenase aroA...”
IUJ47_RS10460 3-phosphoshikimate 1-carboxyvinyltransferase from Enterococcus faecalis
51% identity, 96% coverage
- Antibacterial Components and Modes of the Methanol-Phase Extract from Commelina communis Linn
Liu, Plants (Basel, Switzerland) 2023 - “...3-dehydroquinate synthase ( IUJ47_RS10445 ), a prephenate dehydrogenase ( IUJ47_RS10455 ), a 3-phosphoshikimate 1-carboxyvinyltransferase ( IUJ47_RS10460 ), a shikimate kinase ( IUJ47_RS10465 ), a chorismate synthase ( IUJ47_RS10450 ), and a prephenate dehydratase ( IUJ47_RS10470 ). Notably, nine DEGs in fatty acid biosynthesis were significantly inhibited...”
- “...biosynthesis IUJ47_RS11275 0.151 Type I 3-dehydroquinate dehydratase IUJ47_RS10445 0.154 3-dehydroquinate synthase IUJ47_RS10455 0.227 Prephenate dehydrogenase IUJ47_RS10460 0.262 3-phosphoshikimate 1-carboxyvinyltransferase IUJ47_RS10465 0.278 Shikimate kinase IUJ47_RS10450 0.3 Chorismate synthase IUJ47_RS10470 0.304 Prephenate dehydratase Carbon fixation IUJ47_RS00315 0.096 Flavocytochrome c pathways in prokaryotes IUJ47_RS01530 0.109 Acetyl-CoA carboxylase IUJ47_RS01515 0.158...”
A6TL04 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Alkaliphilus metalliredigens (see paper)
50% identity, 94% coverage
B8CWX4 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Halothermothrix orenii (see paper)
49% identity, 94% coverage
SSU0557 3-phosphoshikimate 1-carboxyvinyltransferase from Streptococcus suis P1/7
51% identity, 94% coverage
AROA_STRPN / Q9S400 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see 2 papers)
Q9S400 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Streptococcus pneumoniae (see paper)
49% identity, 94% coverage
- function: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256)
subunit: Homotetramer. - First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...Bacillus subtilis (P20691), Streptococcus pneumoniae (Q9S400), Staphylococcus aureus (Q05615), Dichelobacter nodosus (Q46550), Halovibrio variabilis (Q1H624),...”
- Crystal structure of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase from the ESKAPE pathogen Acinetobacter baumannii
Sutton, Acta crystallographica. Section F, Structural biology communications 2016 - “...Pseudomonas stutzeri A1501 (ABP79994), Streptococcus pneumoniae (Q9S400) and Staphylococcus aureus (Q05615).] (c) The sequence of the corresponding region...”
- A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity
Peng, PloS one 2012 - “...(P56952), A. tumefaciens strain CP4 (Q9R4E4), Bacillus subtilis (P20691), S. aureus (Q05615), and S. pneumoniae (Q9S400). Growth of cells in the presence of glyphosate The growth curves of the E. coli aroA-deleted mutant ER2799 harboring plasmid p251-AroA-Ra or p251-AroA-Ec are shown in Fig. 3 . Cells...”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...nodosus (Swiss-Prot Q46550), and Streptococcus pneumoniae (Swiss-Prot Q9S400). VOL. 76, 2010 A NOVEL aroA GENE FROM OCHROBACTRUM ANTHROPI 6003 AroA enzymes....”
- Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Sun, Applied and environmental microbiology 2005 - “...(Q05615), Dichelobacter nodosus (Q46550), and Streptococcus pneumoniae (Q9S400). VOL. 71, 2005 NOVEL AroA FROM AN EXTREMELY POLLUTED ENVIRONMENT incubated at...”
T303_04400 3-phosphoshikimate 1-carboxyvinyltransferase from Streptococcus thermophilus ASCC 1275
49% identity, 94% coverage
- Transcriptomic Insights Into the Growth Phase- and Sugar-Associated Changes in the Exopolysaccharide Production of a High EPS-Producing Streptococcus thermophilus ASCC 1275
Padmanabhan, Frontiers in microbiology 2018 - “.... At 5 h, genes involved in phenyl alanine biosynthesis (T303_04365, T303_04370, T303_04375, T303_04380, T303_04385, T303_04400, T303_04405, T303_04410) were found to be significantly upregulated in M17-G, but these genes were downregulated in M17-S and M17-L. Sucrose and lactose supplemented media had higher number of upregulated genes...”
1rf4A / Q9S400 Structural studies of streptococcus pneumoniae epsp synthase, tetrahedral intermediate bound state (see paper)
49% identity, 94% coverage
- Ligand: (3r,4s,5r)-5-{[(1r)-1-carboxy-2-fluoro-1-(phosphonooxy)ethyl]oxy}-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid (1rf4A)
lmo1923 similar to 5-enolpyruvylshikimate-3-phosphate synthase from Listeria monocytogenes EGD-e
47% identity, 94% coverage
- Integrative genomic analysis identifies isoleucine and CodY as regulators of Listeria monocytogenes virulence
Lobel, PLoS genetics 2012 - “...active during intracellular growth. Gene name LMRG identifier (lmo identifier) Enzyme description Pathway aroE 01070.6 (lmo1923) 3-phosphoshikimate 1-carboxyvinyltransferase Common pathway of aromatic compounds biosynthesis purH 02506.9 (lmo1765) IMP cyclohydrolase De novo purine biosynthesis rhaB 02420.6 (lmo2849) rhamnulokinase L-rhamnose utilization hisC 01072.6 (lmo1925) histidinol-phosphate aminotransferase Histidine biosynthesis...”
PP1770 prephenate dehydrogenase, putative/3-phosphoshikimate 1-carboxyvinyltransferase from Pseudomonas putida KT2440
51% identity, 55% coverage
- Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440
Molina-Henares, Microbial biotechnology 2009 - “...(indole3glycerolphosphate synthase), TrpA (tryptophan synthase alphasubunit), TrpB (tryptophan synthase subunit beta), PheA (chorismate mutase/prephenate dehydratase), PP1770 or TyrA (prephenate dehydrogenase, putative/3phosphoshikimate 1carboxyvinyltransferase), TyrB1 (aromaticaminoacid aminotransferase) and PhhA (phenylalanine4hydrolase). Figure 2 Detailed biosynthetic pathway for tryptophan biosynthesis. Details are as in the legend for Fig.1 . This...”
- “...restored by phenylalanine or tyrosine, the miniTn5 was inserted at the 84th codon of ORF PP1770 ( tyrA ). We then tested whether phenylpyruvate or 4hydroxyphenylpyruvate could replace the requirement for phenylalanine or tyrosine in all of the above pheA and tyrA mutants. As expected, all...”
- Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: genetic and physiological basis for improved production
Wierckx, Journal of bacteriology 2008 - “...PP0074 (aroE-1), PP3002 (aroE-2), PP1769 (pheA), PP2170, PP1770, PP4490 (phhA), PP1972 (tyrB-1), PP3590 (tyrB-2), and PP4621 (hmgA). Expression profiles of...”
- Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium
Barbe, Nucleic acids research 2004 - “...fusion is also present in P.aeruginosa (PA3164) and P.putida (PP1770) but not in P.syringae in which the ortholog to ACIAD2222 (PSPTO1748) aligns only on the...”
Q1H624 3-phosphoshikimate 1-carboxyvinyltransferase (Fragment) from Halovibrio variabilis
50% identity, 96% coverage
PA3164 still frameshift 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE prephenate dehydrogenase from Pseudomonas aeruginosa PAO1
49% identity, 58% coverage
- Reconciling high-throughput gene essentiality data with metabolic network reconstructions
Blazier, PLoS computational biology 2019 - “...PA2964 pabC 4-Amino-4-deoxychorismate lyase Cofactors and Vitamins PA2969 plsX Fatty acid biosynthesis protein PlsX Lipid PA3164 Frameshift 3-phosphoshikimate-carboxyvinyltransferase prephenate dehydrogenase Amino Acid PA3296 phoA Alkaline phosphatase Cofactors and Vitamins PA3333 fabH2 3-Oxoacyl-[acyl-carrier-protein] synthase III Lipid PA3633 ygbP 4-Diphosphocytidyl-2-C-methylerythritol synthase Lipid PA3659 dapC Succinyldiaminopimelate transaminase Amino Acid...”
- Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers
Yasuda, PloS one 2017 - “...1 PA3818 extragenic suppressor protein SuhB suhB NP_252507.1 1 PA0316 D-3-phosphoglycerate dehydrogenase serA NP_249007.1 1 PA3164 still frameshift 3-phosphoshikimate 1-carboxyvinyltransferase aroA AE_004091.2 2 PA3763 phosphoribosylformylglycinamidine synthase purL NP_252452.1 1 PA5015 pyruvate dehydrogenase aceE NP_253702.1 3 PA0413 component of chemotactic signal transduction system chpA NP_249104.1 2 PA3115...”
- Dissection of the cis-2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
Rahmani-Badi, Frontiers in microbiology 2015 - “...nadBE, dapB, trpE, aotJ, phhA, phaF, PA0006, PA0400, PA0440, PA0530, PA1339-PA1342, PA1638, PA2084, PA2108, PA2740, PA3164, PA3271, PA3538, PA3589, PA3871, PA4180, PA4672, PA4774, PA4977, PA5093, PA5522 Amino acid and Fatty acid metabolism gcdH Carbohydrate transport and metabolism rpe, eno, ppsA, tpiA, pykF, pgm, chiC, PA3430 Nucleotide...”
- Pseudomonas aeruginosa Genome Evolution in Patients and under the Hospital Environment
Lucchetti-Miganeh, Pathogens (Basel, Switzerland) 2014 - “...contains a right handed beta helix region that shares some similarity with pectate lyases. The PA3164 gene which in PAO1 strain seems to be a pseudogene due to frameshift is probably encoding a functional 3-phosphoshikimate 1-carboxyvinyltransferase prephenate dehydrogenase in both ST395 strains. Other discrete variations were...”
- A systems-level approach for investigating Pseudomonas aeruginosa biofilm formation
Xu, PloS one 2013 - “...21 1 1 PA1681( aroC ) 22 1 1 PA0548( tktA ) 23 1 1 PA3164( pseudogene ) 24 1 1 PA0330( rpiA ) 25 1 1 PA5038( aroB ) 26 2 1 PA4031( ppa ) 27 3 1 PA0904( lysC ) 28 4 1 PA2023(...”
- Regulatory and metabolic networks for the adaptation of Pseudomonas aeruginosa biofilms to urinary tract-like conditions
Tielen, PloS one 2013 - “...can be converted to chorismate in two sequential steps via the phenate dehydrogenase encoded by PA3164 and the chorismate dehydrogenase encoded by PA5184. Again, both genes were found to be induced. Subsequently, chorismate is converted by the anthranilate synthase PhnA to anthranilate, the direct precursor of...”
- A systems biology approach to drug targets in Pseudomonas aeruginosa biofilm
Sigurdsson, PloS one 2012 - “...metabolism UreB (PA4867) ArgB (PA5323) Aromatic amino acids AroC (PA1681), AroK (PA5039) AroB (PA5038), AroA (PA3164), AroE (PA0025) TrpA (PA0035), TrpB (PA0036), PheA (PA3166) Biosynthesis of cofactors, prosthetic groups and carriers, cell wall/ Lipopolysaccharide/ capsule UppS (PA3652) Biotin biosynthesis AccC (PA4848) Branched chain amino acid biosynthesis...”
- Identification of Pseudomonas aeruginosa genes involved in virulence and anaerobic growth
Filiatrault, Infection and immunity 2006 - “...norD PA1850 PA2637 PA2638 PA2639 PA2641 PA2642 PA2644 PA2645 PA2646 PA2648 PA2649 PA3164 1 1 2 2 1 4 2 1 1 5 1 1 PA3912 PA3913 PA3918 PA4049 PA5497 1 1 1 1 2...”
- “...PA2639 PA2641 PA2642 PA2644 PA2645 PA2646 PA2648 PA2649 PA3164 PA3912 PA3913 PA3918 PA4049 PA5497 Gene Descriptionb nirQ norC norB norD Regulatory protein NirQ...”
- More
PA14_23310 EPSP synthase/prephenate dehydrogenase from Pseudomonas aeruginosa UCBPP-PA14
49% identity, 58% coverage
ZMO1796 3-phosphoshikimate 1-carboxyvinyltransferase from Zymomonas mobilis subsp. mobilis ZM4
48% identity, 95% coverage
- Model-driven analysis of mutant fitness experiments improves genome-scale metabolic models of Zymomonas mobilis ZM4
Ong, PLoS computational biology 2020 - “...4 ZMO1198, ZMO1879, ZMO1903 Porphyrinogen Biosynthesis M2 0.859 3 CHORS_f, PSCVT_f, SHKK_f 3 ZMO0594, ZMO1693, ZMO1796 Chorismate biosynthesis M3 0.846 4 ASPCT_f, DHORTS_r , OMPDC_f, ORPT_r 3 ZMO0587, ZMO0791, ZMO1707 Uridine biosynthesis Modules associated with histidine and cofactor biosynthesis M10 0.746 10 ATPPRT_f, HISTD_f, HISTP_f ,...”
PSF113_1640 bifunctional prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase from Pseudomonas ogarae
50% identity, 57% coverage
Pfl01_4074 bifunctional cyclohexadienyl dehydrogenase/ 3-phosphoshikimate 1-carboxyvinyltransferase from Pseudomonas fluorescens Pf0-1
50% identity, 57% coverage
ELZ14_08510, PSEBR_a1552 bifunctional prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase from Pseudomonas brassicacearum
50% identity, 57% coverage
ACIAD2222 bifunctional protein [Includes: putative prephenate or cyclohexadienyl dehydrogenase; 3-phosphoshikimate 1-carboxyvinyltransferase (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS) (AroA)] from Acinetobacter sp. ADP1
47% identity, 55% coverage
YP_003433377 3-phosphoshikimate 1-carboxyvinyltransferase from Hydrogenobacter thermophilus TK-6
46% identity, 94% coverage
- Phylogenetic position of aquificales based on the whole genome sequences of six aquificales species
Oshima, International journal of evolutionary biology 2012 - “...aminopeptidase YP_003432408 methionyl-tRNA synthetase YP_003432463 fatty acid/phospholipid synthesis protein YP_003433015 carboxyl-terminal protease YP_003433058 rRNA methylase YP_003433377 3-phosphoshikimate 1-carboxyvinyltransferase YP_003433542 arginyl-tRNA synthetase YP_003431843 F0F1-type ATP synthase gamma subunit YP_003431889 signal recognition particle GTPase YP_003432507 ribosomal protein L22 YP_003432144 Holliday junction resolvase YP_003432824 DNA processing protein YP_003432257 GTP-binding...”
A1S_2276 hypothetical protein from Acinetobacter baumannii ATCC 17978
46% identity, 58% coverage
ABUW_1366 bifunctional prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase from Acinetobacter baumannii
46% identity, 55% coverage
A0A090C137 3-phosphoshikimate 1-carboxyvinyltransferase from Acinetobacter baumannii
AB57_2630 hypothetical protein from Acinetobacter baumannii AB0057
ABBFA_001168 3-phosphoshikimate 1-carboxyvinyltransferase from Acinetobacter baumannii AB307-0294
46% identity, 54% coverage
MCR_0888 bifunctional prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase from Moraxella catarrhalis BBH18
47% identity, 55% coverage
AROA_COXBU / Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Coxiella burnetii (see paper)
NP_819558 3-phosphoshikimate 1-carboxyvinyltransferase from Coxiella burnetii RSA 493
48% identity, 92% coverage
3slhD / Q83E11 1.70 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
48% identity, 92% coverage
- Ligands: glyphosate; shikimate-3-phosphate; (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid (3slhD)
E2P69_RS07515 3-phosphoshikimate 1-carboxyvinyltransferase from Xanthomonas perforans
48% identity, 95% coverage
XHV734_3365 3-phosphoshikimate 1-carboxyvinyltransferase from Xanthomonas hortorum pv. vitians
48% identity, 93% coverage
A6WUV8 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Brucella anthropi (see paper)
45% identity, 93% coverage
lp_2035 3-phosphoshikimate 1-carboxyvinyltransferase from Lactobacillus plantarum WCFS1
lp_2035 3-phosphoshikimate 1-carboxyvinyltransferase from Lactiplantibacillus plantarum WCFS1
43% identity, 96% coverage
- Molecular Responses of Lactobacilli to Plant Phenolic Compounds: A Comparative Review of the Mechanisms Involved
López, Antioxidants (Basel, Switzerland) 2021 - “...: alkaline shock protein p -CA + Human small intestine passage [ 92 ] lp_2034 lp_2035 lp_2037 tyrA ; prephenate dehydrogenase aroE ; 3-phosphoshikimate 1-carboxyvinyltransferase aroF ; chorismate synthase p -CA p -CA p -CA + + + Human small intestine passage [ 92 ] lp_1744...”
- Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study
Petrov, Genes 2021 - “...2.29 lp_3487, galM3 Galactose mutarotase +2.21 2.19 Ym1downregulated 8-1upregulated lp_2037, aroF Chorismate synthase 2.04 +2.61 lp_2035, aroE 3-P-shikimate 1-carboxyvinyltransferase 2.27 +2.52 lp_2113 Iron-sulfur cluster biosynthesis protein 2.36 +2.17 lp_1992 Hypothetical protein 2.02 +2.09 lp_2038 MFS transporter 2.17 +2.42 lp_1955 ABC transporter permease subunit 3.19 +5.81 *...”
- Transcriptional Reprogramming at Genome-Scale of Lactobacillus plantarum WCFS1 in Response to Olive Oil Challenge
Esteban-Torres, Frontiers in microbiology 2017 - “...H(+)-transporting two-sector ATPase, B subunit 0.95 3.04 lp_1449 Cell surface protein, CscB family 1.27 3.53 lp_2035 aroE 3-phosphoshikimate 1-carboxyvinyltransferase 1.34 3.71 lp_0265 pts5ABC PTS system trehalose-specific transporter subunit IIBC 2.16 3.23 lp_0007 gyrA DNA gyrase subunit A 0.69 2.93 Linear regression fit analysis was applied and...”
- “...In addition, other genes related to respiration metabolism such as six genes ( lp_2033, lp_2034, lp_2035, lp_2036, lp_2037, lp_1084 ) involved in the synthesis of chorismate, which is the menaquinone precursor molecule, and two genes involved in the terpenoid backbone biosynthesis which are also required for...”
- Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays
Saulnier, Applied and environmental microbiology 2007 - “...lp_0256 lp_0529 lp_1084 lp_1085 lp_1169 lp_2033 lp_2034 lp_2035 lp_2037 lp_2551 lp_2553 lp_2556 lp_2557 lp_2559 lp_2830 lp_3493 lp_3494 Cysteine synthase...”
TTHA0457 3-phosphoshikimate 1-carboxyvinyltransferase from Thermus thermophilus HB8
48% identity, 94% coverage
AROA_AGRSC / Q9R4E4 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; CP4 EPSP synthase; EC 2.5.1.19 from Agrobacterium sp. (strain CP4) (see 2 papers)
Q9R4E4 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Agrobacterium sp. (see paper)
46% identity, 92% coverage
- function: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256)
subunit: Monomer. - First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...Halovibrio variabilis (Q1H624), Agrobacterium sp. CP4 (Q9R4E4), Achromobacter sp. LBAA (P0A2Y5), Ochrobactrum anthropi (A6WUV8). P6BS-III is indicated with...”
- A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity
Peng, PloS one 2012 - “...Class II AroA proteins were obtained from Pseudomonas strain PG2982 (P56952), A. tumefaciens strain CP4 (Q9R4E4), Bacillus subtilis (P20691), S. aureus (Q05615), and S. pneumoniae (Q9S400). Growth of cells in the presence of glyphosate The growth curves of the E. coli aroA-deleted mutant ER2799 harboring plasmid...”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...P56952), A. tumefaciens CP4 (Swiss-Prot Q9R4E4), Bacillus subtilis (Swiss-Prot P20691), Staphylococcus aureus (Swiss-Prot Q05615), Dichelobacter nodosus...”
- Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Sun, Applied and environmental microbiology 2005 - “...strain PG2982 (P56952), Agrobacterium tumefaciens CP4 (Q9R4E4), Bacillus subtilis (P20691), Staphylococcus aureus (Q05615), Dichelobacter nodosus (Q46550), and...”
Q71LY8 3-phosphoshikimate 1-carboxyvinyltransferase from Glycine max
46% identity, 92% coverage
2gg6A / Q9R4E4 Cp4 epsp synthase liganded with s3p (see paper)
46% identity, 94% coverage
- Ligand: shikimate-3-phosphate (2gg6A)
BB562_07550 3-phosphoshikimate 1-carboxyvinyltransferase from Lactiplantibacillus pentosus
45% identity, 94% coverage
- Transcriptomic Profile and Probiotic Properties of Lactiplantibacillus pentosus Pre-adapted to Edible Oils
Alonso, Frontiers in microbiology 2021 - “...methionine metabolism); histidinol-phosphatase (BB562_04885; histidine metabolism); and genes coding for chorismate synthase (BB562_07540), 3-phosphoshikimate 1-carboxyvinyltransferase (BB562_07550), and shikimate dehydrogenase (BB562_11875), representing phenylalanine, tyrosine, and tryptophan biosyntheses, respectively. However, the down-regulated genes corresponded to asparagine synthase (BB562_02530; asparagine synthesis and glutamine hydrolysis; Supplementary Table 1 and Supplementary...”
- “...pathway, glucose-1-phosphate adenylyltransferase (BB562_00090) involved in amino sugar and nucleotide sugar metabolism, and 3-phosphoshikimate 1-carboxyvinyltransferase (BB562_07550) and shikimate dehydrogenase (BB562_11875) implicated in phenylalanine, tyrosine, and tryptophan biosynthesis ( Supplementary Table 2 and Supplementary Figure 3 ). Concerning other metabolic pathways ( Supplementary Figure 3 ), similar...”
BAAA_1000022 3-phosphoshikimate 1-carboxyvinyltransferase from Brucella abortus str. 2308 A
BAB1_0023 ATP/GTP-binding site motif A (P-loop):EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase):3-phosphoshikimate 1-carbo... from Brucella melitensis biovar Abortus 2308
45% identity, 93% coverage
Q46550 3-phosphoshikimate 1-carboxyvinyltransferase from Dichelobacter nodosus
46% identity, 92% coverage
- First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...(Q9S400), Staphylococcus aureus (Q05615), Dichelobacter nodosus (Q46550), Halovibrio variabilis (Q1H624), Agrobacterium sp. CP4 (Q9R4E4), Achromobacter sp. LBAA...”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...aureus (Swiss-Prot Q05615), Dichelobacter nodosus (Swiss-Prot Q46550), and Streptococcus pneumoniae (Swiss-Prot Q9S400). VOL. 76, 2010 A NOVEL aroA...”
- Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Sun, Applied and environmental microbiology 2005 - “...subtilis (P20691), Staphylococcus aureus (Q05615), Dichelobacter nodosus (Q46550), and Streptococcus pneumoniae (Q9S400). VOL. 71, 2005 NOVEL AroA FROM AN...”
C3MC80 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Sinorhizobium fredii (see paper)
45% identity, 94% coverage
AWH67_RS01510 3-phosphoshikimate 1-carboxyvinyltransferase from Bartonella bacilliformis
45% identity, 94% coverage
SAPIG1464 3-phosphoshikimate 1-carboxyvinyltransferase from Staphylococcus aureus subsp. aureus ST398
45% identity, 94% coverage
B488_RS00545 3-phosphoshikimate 1-carboxyvinyltransferase from Liberibacter crescens BT-1
44% identity, 93% coverage
SAOUHSC_01481 3-phosphoshikimate 1-carboxyvinyltransferase from Staphylococcus aureus subsp. aureus NCTC 8325
Q05615 3-phosphoshikimate 1-carboxyvinyltransferase from Staphylococcus aureus (strain NCTC 8325 / PS 47)
MW1354 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE from Staphylococcus aureus subsp. aureus MW2
45% identity, 94% coverage
- A new platform for ultra-high density Staphylococcus aureus transposon libraries
Santiago, BMC genomics 2015 - “...were significantly enriched in seven genes in the aromatic amino acid and menaquinol biosynthetic pathways (SAOUHSC_01481, aroB: SAOUHSC_01482, aroF: SAOUHSC_01483, menF: SAOUHSC_00982, menD: SAOUHSC_00983, aroE: SAOUHSC_01699, and aroA: SAOUHSC_01852) (Figure 7 A). In addition to producing phenylalanine and tyrosine, the aromatic amino acid pathway provides precursors...”
- First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...Streptococcus pneumoniae (Q9S400), Staphylococcus aureus (Q05615), Dichelobacter nodosus (Q46550), Halovibrio variabilis (Q1H624), Agrobacterium sp. CP4...”
- A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity
Peng, PloS one 2012 - “...from Pseudomonas strain PG2982 (P56952), A. tumefaciens strain CP4 (Q9R4E4), Bacillus subtilis (P20691), S. aureus (Q05615), and S. pneumoniae (Q9S400). Growth of cells in the presence of glyphosate The growth curves of the E. coli aroA-deleted mutant ER2799 harboring plasmid p251-AroA-Ra or p251-AroA-Ec are shown in...”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...(Swiss-Prot P20691), Staphylococcus aureus (Swiss-Prot Q05615), Dichelobacter nodosus (Swiss-Prot Q46550), and Streptococcus pneumoniae (Swiss-Prot Q9S400)....”
- Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Sun, Applied and environmental microbiology 2005 - “...(Q9R4E4), Bacillus subtilis (P20691), Staphylococcus aureus (Q05615), Dichelobacter nodosus (Q46550), and Streptococcus pneumoniae (Q9S400). VOL. 71, 2005 NOVEL...”
- Nucleotide substitution and recombination at orthologous loci in Staphylococcus aureus
Hughes, Journal of bacteriology 2005 - “...MW0518, MW0551, MW0764, MW0862, MW1038, MW1206, MW1328, MW1354, MW1355, MW1356, MW1738, MW1889, MW1895, MW1928, MW1932, MW1962, MW2132, MW2254, MW2320, MW2321,...”
SAV1464 3-phosphoshikimate 1-carboxyvinyltransferase from Staphylococcus aureus subsp. aureus Mu50
SA1297 3-phosphoshikimate 1-carboxyvinyltransferase from Staphylococcus aureus subsp. aureus N315
43% identity, 97% coverage
SE1153 3-phosphoshikimate 1-carboxyvinyltransferase from Staphylococcus epidermidis ATCC 12228
43% identity, 94% coverage
XF2324 3-phosphoshikimate 1-carboxyvinyltransferase from Xylella fastidiosa 9a5c
43% identity, 94% coverage
- Xylella fastidiosa gene expression analysis by DNA microarrays
Travensolo, Genetics and molecular biology 2009 - “...XF0267 pspB Serine protease -1.02 XF2219 hisD Histidinol dehydrogenase -0.81 XF0624 aroE Shikimate 5-dehydrogenase -0.75 XF2324 aroE 3-phosphoshikimate 1-carboxyvinyltransferase -0.71 XF1915 trpG Anthranilate synthase component II -0.64 Biosynthesis of cofactors, prosthetic groups and regulatory functions XF0956 thiL Thiamine-monophosphate kinase 0.93 XF2592 phoR Two-component system, sensor protein...”
aroA / AAA71897.1 3-phosphoshikimate-1-carboxyvinyltransferase from Staphylococcus aureus (see paper)
44% identity, 94% coverage
Cj0895c 3-phosphoshikimate 1-carboxyvinyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168
P0C630 3-phosphoshikimate 1-carboxyvinyltransferase from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
39% identity, 92% coverage
- In vivo and in silico determination of essential genes of Campylobacter jejuni
Metris, BMC genomics 2011 - “...cj1291c, cj1531, cj1535c FBA & the 2 transposon mutagenesis methods cj0231c, cj0545, cj0707, cj0810, cj0855, cj0895c, cj1644, cj1676 The "metabolic genes" refer to the genes that were included in the FBA model. This table gives the list of the genes shown in Figure 3. The genes...”
- “...number of disparate pathways ( cj0231c (NrdF), cj0545 (HemC), cj0707 (KdtA), cj0810 (NadE), cj0855 (FolD), cj0895c (AroA), cj1644 (IspA), cj1676 (MurB)). An analysis of the intersection genes, with respect to the KEGG, JCVI, and BioCyc databases, shows clusters of genes associated with: purine and pyrimidine metabolism,...”
- In Silico Screening and Analysis of Broad-Spectrum Molecular Targets and Lead Compounds for Diarrhea Therapy
Ugboko, Bioinformatics and biology insights 2019 - “...Q9PP65 UDP-N-acetylenolpyruvoylglucosamine reductase murB Q9PM01 Aminodeoxyfutalosine nucleosidase pfs Q0PC20 1-Deoxy-D-xylulose-5-phosphate reductoisomerase dxr Q9PMV3 3-Phosphoshikimate-1-carboxyvinyltransferase aroA P0C630 Ribose-5-phosphate isomerase rpiB Q0P9X4 Penicillin binding protein pbpC Q0PAL6 Chemotaxis protein cheA cheV Q0PBM1 Cb-type cytochrome C oxidase subunit I ccoN Q0P8C7 Protein translocase subunit SecD secD Q0P9G1 2 DNA...”
- Novel Drug Targets for Food-Borne Pathogen Campylobacter jejuni: An Integrated Subtractive Genomics and Comparative Metabolic Pathway Study
Mehla, Omics : a journal of integrative biology 2015 - “...Q0PC20 Q9PIK3 Q9PIK2 Q9PIK1 Q0PBJ3 Q0PBA5 Q9PI11 P0C632 P0C630 Q9PNT2 Q9PMV3 Q0P8N9 Q9PM41 Q9PIT2 Q0PAS0 Q0PB07 Q9PIM1 Q9PHU0 Q9PIZ5 Q9PP65 Q9PM01 Q9PNN7 Q0PA95...”
- “...Gene name ispH aroA 20 P0C632 21 P0C630 4-Hydroxytetrahydrodipicolinate reductase 26 Q9PIT2 27 Q0P9X4 rpiB Ribose 5-phosphate isomerase Chorismate synthase dapB...”
P56197 3-phosphoshikimate 1-carboxyvinyltransferase from Helicobacter pylori (strain ATCC 700392 / 26695)
HP0401 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) from Helicobacter pylori 26695
38% identity, 92% coverage
R7B7W1 3-phosphoshikimate 1-carboxyvinyltransferase from Eggerthella sp. CAG:298
37% identity, 97% coverage
TK0263 5-enolpyruvylshikimate-3-phosphate synthase from Thermococcus kodakaraensis KOD1
34% identity, 94% coverage
- A novel DNA nuclease is stimulated by association with the GINS complex
Li, Nucleic acids research 2011 - “...reductase TK2270 29 7456 2 30.2 Unknown TK2255 28 48783 2 3 Bifunctional phosphatase/dolichol-phosphate glucosyltransferase TK0263 27 43206 2 3.5 3-phosphoshikimate 1-carboxyvinyltransferase Proteins with at least two peptides matches are listed with their molecular weight, MASCOT score, and the percentage of the amino acid sequence covered...”
TON_1138 5-enolpyruvylshikimate-3-phosphate synthase from Thermococcus onnurineus NA1
32% identity, 94% coverage
- Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate
Jung, Scientific reports 2017 - “...1 ). The base substitution occurred at genes encoding aromatic amino acid permease (TON_0820), 3-phosphoshikimate-1-carboxyvinyltransferase (TON_1138), signal peptidase (TON_1555), F 420 -reducing hydrogenase subunit (TON_1561), formate transporter (TON_1573), hypothetical proteins (TON_0618, TON_1084, TON_1641, TON_RS08535) and noncoding regions between amino-acid transporter and biotin-protein ligase (TON_0901 -TON_0902) and...”
- “...strain. Locus_tag Location a Mutational change Product description TON_0820 G473A G158D Aromatic amino acid permease TON_1138 G993A G331G 3-Phosphoshikimate 1-carboxyvinyltransferase TON_1555 C485T P162L Signal peptidase TON_1573 G154A A52T Formate transporter TON_1641 C255T D85D Hypothetical protein TON_RS08535 G257A G86E Hypothetical protein TON_0618 T946 deletion Frame shift Hypothetical...”
CAC0895 5-enolpyruvylshikimate-3-phosphate synthase from Clostridium acetobutylicum ATCC 824
32% identity, 97% coverage
- Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis
Senger, Biotechnology and bioengineering 2008 - “...CAC1825, CAC0390, CAC0391, CAC3348/CAC0578. List 6: CAC0737/CAC1001/CAC1819/CAC2832/CAC0764/CAC1673/CAC1674. List 7: (from DAHP) CAC0894, CAC0713/CAC0899, CAC0897, CAC0898, CAC0895, CAC0896. List 8: CAC2680, CAC0944, CAC1348, CAC1730, CAC0726, CAC0819/CAC3221. List 9: CAC0936, CAC0943, CAC0942, CAC0940, CAC0939, CAC0938, CAC1369, CAC2727, CAC0937. List 10: CAC3162/CAC3163, CAC3161, CAC3159, CAC3160, CAC3157/CAC3158. List 11: CAC1234,...”
- Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum
Alsaker, Journal of bacteriology 2005 - “...(CAC0896 and CAC0898), t3 (CAC0897), and t4 (CAC0892 to CAC0895 and CAC899), and the tryptophan formation genes are grouped in clusters t4 (CAC3159 to CAC3163),...”
Q482G5 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Colwellia psychrerythraea (see paper)
30% identity, 94% coverage
SO_2404 3-phosphoshikimate 1-carboxyvinyltransferase from Shewanella oneidensis MR-1
30% identity, 96% coverage
PF1699 3-phosphoshikimate 1-carboxyvinyltransferase from Pyrococcus furiosus DSM 3638
31% identity, 98% coverage
SLI_1096 3-phosphoshikimate 1-carboxyvinyltransferase from Streptomyces lividans 1326
30% identity, 93% coverage
- Phylogenomic Analysis of Natural Products Biosynthetic Gene Clusters Allows Discovery of Arseno-Organic Metabolites in Model Streptomycetes
Cruz-Morales, Genome biology and evolution 2016 - “...last accessed on June 2, 2016. Mutagenesis Analysis Streptomyces coelicolor (SCO6819) and S. lividans 66 (SLI_1096) knock-out mutants were constructed using in-frame PCR-targeted gene replacement of their coding sequences with an apramycin resistance cassette ( acc(3) IV) ( Gust et al. 2003 ). The plasmid pIJ773...”
- “...orthologous region in S. lividans , this cosmid clone was also used for disruption of SLI_1096. The gene disruptions were performed using the Redirect system ( Gust et al. 2003 ). Double cross-over ex-conjugants were selected using apramycin resistance and kanamycin sensitivity as phenotypic markers. The...”
AROA_VIBCH / Q9KRB0 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Q9KRB0 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Vibrio cholerae serotype O1 (see paper)
3nvsA / Q9KRB0 1.02 angstrom resolution crystal structure of 3-phosphoshikimate 1- carboxyvinyltransferase from vibrio cholerae in complex with shikimate-3-phosphate (partially photolyzed) and glyphosate
C3LN54 3-phosphoshikimate 1-carboxyvinyltransferase from Vibrio cholerae serotype O1 (strain M66-2)
VC1732 3-phosphoshikimate 1-carboxyvinyltransferase from Vibrio cholerae O1 biovar eltor str. N16961
31% identity, 96% coverage
- function: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256)
subunit: Homotetramer. - Ligands: shikimate-3-phosphate; (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid; glyphosate; magnesium ion (3nvsA)
- Bioinformatics-based Characterization of the Sequence Variability of Zika Virus Polyprotein and Envelope Protein (E).
Polanco, Evolutionary bioinformatics online 2022 - “...P0C987, Q65215, Q8XD86, Q9GKL2, Q13B10, Q9ZBD8, E9D269, Q8ZRP8, P53678, P97784, P53677, P75464, Q32Q86, Q96PY5, C3LN54, Q9KRB0, P29365, Q29003, Q6G2QB, P05490, Q11QT1, P99117, P67472, A5IW31, A6U4Y6, A7X6Y6, A7TMJ6, P19833, Q9GZU1, P44961, Q99K10, B1JM25, P68022, P68025, P68023, P68024, P68021, P68020, P68019, P68016, P68018, P68017, Q632I5, P54886, F5HIN9, P16728,...”
- Bioinformatics-based Characterization of the Sequence Variability of Zika Virus Polyprotein and Envelope Protein (E).
Polanco, Evolutionary bioinformatics online 2022 - “...Q0BV25, P0C987, Q65215, Q8XD86, Q9GKL2, Q13B10, Q9ZBD8, E9D269, Q8ZRP8, P53678, P97784, P53677, P75464, Q32Q86, Q96PY5, C3LN54, Q9KRB0, P29365, Q29003, Q6G2QB, P05490, Q11QT1, P99117, P67472, A5IW31, A6U4Y6, A7X6Y6, A7TMJ6, P19833, Q9GZU1, P44961, Q99K10, B1JM25, P68022, P68025, P68023, P68024, P68021, P68020, P68019, P68016, P68018, P68017, Q632I5, P54886, F5HIN9,...”
- Identification of the TcpP-binding site in the toxT promoter of Vibrio cholerae and the role of ToxR in TcpP-mediated activation
Goss, Infection and immunity 2010 - “...were within genes, VC1542 (DNA ligase) and VC1732 (3-phosphoshikimate 1-carboxyvinyltransferase), whereas one was between the hypothetical open reading frames...”
SCO6819 3-phosphoshikimate 1-carboxyvinyltransferase from Streptomyces coelicolor A3(2)
30% identity, 94% coverage
- Coelimycin Synthesis Activatory Proteins Are Key Regulators of Specialized Metabolism and Precursor Flux in Streptomyces coelicolor A3(2)
Bednarz, Frontiers in microbiology 2021 - “...is predicted to be a polar arseno-polyketide and its synthesis was abolished by deletion of SCO6819 gene. In our studies, all detected proteins from this cluster, including SCO6819, were virtually absent from the proteomes of cpkN and cpkO strains. Our results also indicate that besides the...”
- The Inhibition of Antibiotic Production in Streptomyces coelicolor Over-Expressing the TetR Regulator SCO3201 IS Correlated With Changes in the Lipidome of the Strain
Zhang, Frontiers in microbiology 2020 - “...Probable anthranilate synthase component II 2.142 0 sco3214 Probable anthranilate synthase component I 1.762 1.719 sco6819 3-phosphoshikimate 1-carboxyvinyltransferase. 1.425 0 Aromatic amino acids degradation sco0199 Putative alcohol dehydrogenase 0 5.466 sco1204 Putative aldehyde dehydrogenase 1.344 0 sco1715 Putative homogentisate 1,2-dioxygenase 1.336 0 sco2700 Tyrosinase (monophenol monooxygenase)...”
- Phylogenomic Analysis of Natural Products Biosynthetic Gene Clusters Allows Discovery of Arseno-Organic Metabolites in Model Streptomycetes
Cruz-Morales, Genome biology and evolution 2016 - “...is available at http://evodivmet.langebio.cinvestav.mx/EvoMining/index.html , last accessed on June 2, 2016. Mutagenesis Analysis Streptomyces coelicolor (SCO6819) and S. lividans 66 (SLI_1096) knock-out mutants were constructed using in-frame PCR-targeted gene replacement of their coding sequences with an apramycin resistance cassette ( acc(3) IV) ( Gust et al....”
- “...In S. coelicolor and S. lividans , these recruited homologs are encoded by SLI_1096 and SCO6819, and they are marked with green crosses in fig. 2 C . These genes are situated only six genes upstream of the two-gene PKS system (SCO6826-7 and SLI_1088-9, respectively) used...”
- Decoding options and accuracy of translation of developmentally regulated UUA codon in Streptomyces: bioinformatic analysis
Rokytskyy, SpringerPlus 2016 - “...XNR_1345 tRNA(Gln,Lys,Glu) U34 2-thiouridylase MiaA SCO5791 XNR_1074 delta(2)-isopentenylpyrophosphatetRNA-adenosine transferase MiaB SCO5787 XNR_1078 tRNA-i(6)A37 methylthiotransferase AroA SCO6819 XNR_1588 5-Enolpyruvylshikimate-3-phosphate synthetase AroB SCO1494 XNR_5357 3-Dehydroquinate synthase AroC SCO1496 XNR_5355 Chorismate synthase AroE SCO1498 XNR_5354 Dehydroshikimatereductase, NAD(P)-binding AroD SCO1961 XNR_4909 3-Dehydroquinate dehydratase AroK SCO1495 XNR_5356 Shikimate kinase I TsaA...”
mycA4 / H9TE88 shimimate dehydratase/3,4-dihydroxycyclohexa-1,5-diene-1-carboxylate--CoA ligase from Streptomyces flaveolus (see 3 papers)
32% identity, 42% coverage
GIY62_15125 3-phosphoshikimate 1-carboxyvinyltransferase from Burkholderia plantarii
31% identity, 93% coverage
bglu_1g08780 Prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase from Burkholderia glumae BGR1
31% identity, 93% coverage
RS_RS04510 3-phosphoshikimate 1-carboxyvinyltransferase from Ralstonia pseudosolanacearum GMI1000
RSc0907 PROBABLE 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE (EPSP SYNTHASE) PROTEIN from Ralstonia solanacearum GMI1000
30% identity, 92% coverage
- A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv. vitians
Morinière, mSystems 2022 - “...pyrB Aspartate carbamoyltransferase ATU_RS06440 Dda3937_01284 XHV734_3113 udg UDP-glucose 6-dehydrogenase ATU_RS12570; ATU_RS19395 XHV734_3365 aroA 3-Phosphoshikimate 1-carboxyvinyltransferase RS_RS04510 XHV734_3366 pheA Chorismate mutase/prephenate dehydratase ATU_RS00480 XHV734_3367 serC 3-Phosphoserine/phosphohydroxythreonine aminotransferase RS_RS04490 XHV734_3398 metXS Homoserine O -succinyltransferase Psyr_0474 XHV734_3771 clpX ATPase and specificity subunit of ClpX-ClpP ATP-dependent serine protease Psyr_1748 RS_RS08650...”
- Genome-Wide Identification of Ralstonia solanacearum Genes Required for Survival in Tomato Plants
Su, mSystems 2021 - “...Genomes (KEGG) database ( Fig.S2 ). Four ( RS_RS14885 , RS_RS07885 , RS_RS13320 , and RS_RS04510 ) of these 10 genes are involved in the shikimate pathway, which synthesizes chorismite, an important biochemical intermediate for amino acid biosynthesis. Four genes are responsible for tryptophan biosynthesis, including...”
- Functional characterization of two 3-dehydroquinases of AroQ1 and AroQ2 in the shikimate pathway and expression of genes for the type III secretion system in Ralstonia solanacearum
Zhang, Frontiers in microbiology 2023 - “...is an important intermediate in the shikimate pathway. Furthermore, shikimate kinase AroK (RSc2970), 1-carboxyvinyltransferase AroA (RSc0907), and chorismate synthase AroC (RSc1566) control the rest three reactions to form chorismic acid, the final product in the shikimate pathway. Further experiments will be performed to confirm whether other...”
FTT_0588 3-phosphoshikimate 1-carboxyvinyltransferase from Francisella tularensis subsp. tularensis SCHU S4
29% identity, 94% coverage
FTN_1091 3-phosphoshikimate 1-carboxyvinyltransferase from Francisella tularensis subsp. novicida U112
29% identity, 94% coverage
- Francisella-arthropod vector interaction and its role in patho-adaptation to infect mammals
Akimana, Frontiers in microbiology 2011 - “...FTN_1058 Trigger factor (TF) protein tig FTN_1066 Metal ion transporter protein FTN_1073 DNA/RNA endonuclease G FTN_1091 3-Phosphoshikimate 1-carboxyvinyltransferase aroA FTN_1099 Transcriptional regulator, LysR family FTN_1115 Type IV pili nucleotide binding protein, ABC transporter, ATP-binding protein pilB FTN_1116 Type IV pili polytopic inner membrane protein pilC FTN_1135...”
- Molecular complexity orchestrates modulation of phagosome biogenesis and escape to the cytosol of macrophages by Francisella tularensis
Asare, Environmental microbiology 2010 - “...protein 2 * tnfn1_pw060420p01q152 FTN_0453 glycosyl transferase 5 tnfn1_pw060419p02q135 FTN_0560 ksgA dimethyladenosine transferase 3 tnfn1_pw060419p04q168 FTN_1091 aroA 3-phosphoshikimate 1-carboxyvinyltransferase 2 tnfn1_pw060418p03q185 FTN_1400 S-adenosylmethionine-dependent methyltransferase 5 tnfn1_pw060418p04q172 FTN_1418 manC mannose-1-phosphate guanylyltransferase 4 DNA Modification tnfn1_pw060510p04q169 FTN_0122 recA recombinase A protein 2 tnfn1_pw060328p06q179 FTN_0492 parC DNA topoisomerase IV...”
- “...family protein tnfn1_pw060418p01q153 FTN_1055 lon DNA-binding, ATP-dependent protease La tnfn1_pw060510p01q114 FTN_1073 DNA/RNA endonuclease G tnfn1_pw060419p04q168 FTN_1091 aroA 3-phosphoshikimate 1-carboxyvinyltransferase tnfn1_pw060328p08q188 FTN_1098 conserved hypothetical membrane protein tnfn1_pw060328p02q109 FTN_1107 metlQ methionine uptake transporter (MUT) family protein, membrane and periplasmic protein tnfn1_pw060328p02q175 FTN_1131 putA bifunctional proline dehydrogenase, pyrroline-5-carboxylate dehydrogenase...”
- Molecular bases of proliferation of Francisella tularensis in arthropod vectors
Asare, Environmental microbiology 2010 - “...protein 2 * tnfn1_pw060420p01q152 FTN_0453 glycosyl transferase 5 tnfn1_pw060419p02q135 FTN_0560 ksgA dimethyladenosine transferase 3 tnfn1_pw060419p04q168 FTN_1091 aroA 3-phosphoshikimate 1-carboxyvinyltransferase 2 tnfn1_pw060418p03q185 FTN_1400 S-adenosylmethionine-dependent methyltransferase 5 tnfn1_pw060418p04q172 FTN_1418 manC mannose-1-phosphate guanylyltransferase 4 DNA Modification tnfn1_pw060510p04q169 FTN_0122 recA recombinase A protein 2 tnfn1_pw060328p06q179 FTN_0492 parC DNA topoisomerase IV...”
- “...family protein tnfn1_pw060418p01q153 FTN_1055 lon DNA-binding, ATP-dependent protease La tnfn1_pw060510p01q114 FTN_1073 DNA/RNA endonuclease G tnfn1_pw060419p04q168 FTN_1091 aroA 3-phosphoshikimate 1- carboxyvinyltransferase tnfn1_pw060328p08q188 FTN_1098 conserved hypothetical membrane protein tnfn1_pw060328p02q109 FTN_1107 metlQ methionine uptake transporter (MUT) family protein, membrane and periplasmic protein tnfn1_pw060328p02q175 FTN_1131 putA bifunctional proline dehydrogenase, pyrroline-5-carboxylate...”
CKR_0704 hypothetical protein from Clostridium kluyveri NBRC 12016
29% identity, 94% coverage
P05466 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic from Arabidopsis thaliana
AT2G45300 3-phosphoshikimate 1-carboxyvinyltransferase / 5-enolpyruvylshikimate-3-phosphate / EPSP synthase from Arabidopsis thaliana
28% identity, 81% coverage
- Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns
Ojosnegros, International journal of molecular sciences 2023 - “...compounds 156554-185_2_ORF1 Q9S777 4CL3 4-COUMARATE-COA LIGASE 3 51.9 2 1 2 0 Secondary compounds 223603-118_1_ORF1 P05466 AT2G45300 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE 44.3 2 1 1 0 Oxido -reduction 133847-212_2_ORF2 Q9SID3 AT2G31350 HYDROXYACYLGLUTATHIONE HYDROLASE 2 33.1 6 1 1 6.51 10 131 Oxido -reduction 34437-432_2_ORF1 Q9M2W2 GSTL2 GLUTATHIONE S-TRANSFERASE...”
- Deciphering the structure of Arabidopsis thaliana 5-enol-pyruvyl-shikimate-3-phosphate synthase: An essential step toward the discovery of novel inhibitors to supersede glyphosate
Ruszkowski, Computational and structural biotechnology journal 2022 - “...Functional features of A. thaliana EPSPS Heterologous expression of A. thaliana EPSPS (AtEPSPS, Uniprot ID: P05466) in E. coli and affinity purification yielded an active enzyme, with a specific activity of 1775158 nkat mg 1 protein, a value notably higher than those reported for other plant...”
- “...the RNeasy Plant Mini Kit (Qiagen). The construct for the overexpression of AtEPSPS (Uniprot ID: P05466; locus At2g45300) was designed based on a comparative analysis of homologous sequences from plants and prediction of signal peptides using the TargetP 1.1 server [58] , [59] . The final...”
- Effects of Phosphonate Herbicides on the Secretions of Plant-Beneficial Compounds by Two Plant Growth-Promoting Soil Bacteria: A Metabolomics Investigation
Li, ACS environmental Au 2022 - “...Knowledgebase ( https://www.uniprot.org/ ). Specifically, we compared an EPSP synthase from the plant Arabidopsis thaliana (P05466) with two EPSP synthases from P. protegens Pf-5 (Q4K8N1, Q4K7P7) and two EPSP synthases from P. megaterium QM B1551 (D5DW56, D5DRE6). We also compared four glutamine synthetases from a wheat...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...Spot number Accession (UP), Gene locus (TAIR) AMINO ACID METABOLISM Synthesis 3-phosphoshikimate 1-carboxyvinyltransferase 51, 52 P05466, At2g45300 4-hydroxy-tetrahydrodipicolinate reductase 1 86 O80574, At2g44040 Acetolactate synthase small subunit 1 58 Q9FFF4, At5g16290 Acetylglutamate kinase 92 Q9SCL7, At3g57560 Acetylornithine aminotransferase 62, 63 Q9M8M7, At1g80600 Argininosuccinate synthase 51, 52...”
- First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...sequences were obtained from: Arabidopsis thaliana (P05466), Brassica napus (P17688), Petunia hybrida (P11043), Solanum lycopersicum (P10748), Bordetella...”
- Physiological and iTRAQ-based proteomic analyses reveal the function of exogenous γ-aminobutyric acid (GABA) in improving tea plant (Camellia sinensis L.) tolerance at cold temperature
Zhu, BMC plant biology 2019 - “...plants. Comparing treatments T3/T1 with T4/T1, the proteins Glycine-rich RNA-binding protein 2 (Q9SVM8), 3-phosphoshikimate 1-carboxyvinyltransferase (P05466), Enolase 1 (Q9C9C4) and Fructose-bisphosphate aldolase 3 (Q9ZU52) had the same expression patterns; and proteins Aldolase superfamily protein (F4IC59), Leucine aminopeptidase 2 (Q944P7), Thiol protease aleurain-like (Q8RWQ9), Reactive Intermediate Deaminase...”
- A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity
Peng, PloS one 2012 - “...Class I AroA proteins were obtained from E. coli (P07638), Aeromonas salmonicida (Q03321), Arabidopsis thaliana (P05466), N. tabacum (P23981), Petunia hybrida (P11043), Z. mays (CAA44974), and Bordetella pertussis (P12421). Class II AroA proteins were obtained from Pseudomonas strain PG2982 (P56952), A. tumefaciens strain CP4 (Q9R4E4), Bacillus...”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...salmonicida (Swiss-Prot Q03321), Arabidopsis thaliana (Swiss-Prot P05466), Nicotiana tabacum (Swiss-Prot P23981), Petunia hybrida (Swiss-Prot P11043), Zea mays...”
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- MurA-catalyzed synthesis of 5-enolpyruvylshikimate-3-phosphate confers glyphosate tolerance in bryophytes
Caygill, Proceedings of the National Academy of Sciences of the United States of America 2024 - “...amino acid sequences, with the chloroplast transit peptides removed, of EPSPS proteins from A. thaliana (AT2G45300) and M. polymorpha (Mp6g04140.1), and MpMurA (Mp5g14110.1) from M. polymorpha were used for reverse transcription, codon optimization, and synthesis of DNA by Twist Bioscience. Expression constructs were transformed into E....”
- Identification and Expression Analysis of EPSPS and BAR Families in Cotton
Li, Plants (Basel, Switzerland) 2023 - “...53 ]. Arabidopsis has two natural EPSPS loci, AtEPSPS1 ( AT1G48860 ) and AtEPSPS2 ( AT2G45300 ), which are highly expressed throughout development [ 54 ]. It was found that overexpression of the natural gene encoding 5-enolpyruvate oxalate synthase (EPSP) may increase the fertility of Arabidopsis...”
- “...reproductive ability of cotton, laying a foundation for further improving cotton. Among them, AtEPSPS2 ( AT2G45300 ) is the upregulated expression gene of Ca 2+ response, and Ca 2+ transient mediates the response to environmental stresses, including salt, drought, cold, heat, ultraviolet, etc., which is the...”
- Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns
Ojosnegros, International journal of molecular sciences 2023 - “...synthesize anthocyanins, flavonoids, isoflavonoids, coumarins, lignin, suberin, and phenols [ 60 ], and 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE (AT2G45300), involved in the synthesis of chorismate, which is the precursor of the amino acids phenylalanine, tryptophan, and tyrosine [ 61 ]. The proteins 4CL3 and the transferases GLUTATHIONE S-TRANSFERASE L2...”
- “...156554-185_2_ORF1 Q9S777 4CL3 4-COUMARATE-COA LIGASE 3 51.9 2 1 2 0 Secondary compounds 223603-118_1_ORF1 P05466 AT2G45300 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE 44.3 2 1 1 0 Oxido -reduction 133847-212_2_ORF2 Q9SID3 AT2G31350 HYDROXYACYLGLUTATHIONE HYDROLASE 2 33.1 6 1 1 6.51 10 131 Oxido -reduction 34437-432_2_ORF1 Q9M2W2 GSTL2 GLUTATHIONE S-TRANSFERASE L2...”
- Label-Free Quantitative Proteomics Reveal the Involvement of PRT6 in Arabidopsis thaliana Seed Responsiveness to Ethylene
Wang, International journal of molecular sciences 2022 - “...hydrolases-like superfamily protein 7.91 AT1G74260 Purine biosynthesis 4 1.61 AT3G57610 Adenylosuccinate synthase (purine synthesis) 1.50 AT2G45300 RNA 3-terminal phosphate cyclase/enolpyruvate transferase%2C alpha/beta 1.80 AT3G52150 RNA-binding (RRM/RBD/RNP motifs) family protein 1.37 AT3G19130 RNA-binding protein 47B 1.86 AT5G63420 RNA-metabolising metallo-beta-lactamase family protein 1.34 AT3G58510 DEA(D/H)-box RNA helicase family...”
- Transcriptome and HPLC Analysis Reveal the Regulatory Mechanisms of Aurantio-Obtusin in Space Environment-Induced Senna obtusifolia Lines
Mao, International journal of environmental research and public health 2022 - “...included AT5G66120 (catalyzes the second step in the shikimate pathway), CM1 (encodes chorismate mutase), and AT2G45300 (catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate) was the most relevant (0.997). DHQS exhibited a close functional partnership with DHS2 (phospho-2-dehydro-3-deoxyheptonate aldolase 2) and MEE32 (bifunctional 3-dehydroquinate dehydratase/shikimate...”
- “...partners: EMB1144 (chorismate synthase) and CM3 (chorismate mutase 3). SKM was most closely linked to AT2G45300, followed by MEE32. The most useful predicted partners for MenB were AAE14 (2-succinylbenzoate-CoA ligase) and AT3G15290 (3-hydroxybutyryl-CoA dehydrogenase-like protein). MenB was also closely linked to DHNAT2 (catalyzes the hydrolysis of...”
- Mapping of a novel clubroot disease resistance locus in Brassica napus and related functional identification
Jiang, Frontiers in plant science 2022 - “...LOC106438246 AT2G45220 88.27 Plant invertase/pectin methylesterase inhibitor superfamily LOC106438247 AT2G45260 85.80 myosin-4 protein (DUF641) LOC106438248 AT2G45300 89.35 RNA 3-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta LOC106438249 AT2G45310 85.23 UDP-D-glucuronate 4-epimerase 4 BNAA03G56780D AT2G45320 87.82 polyphosphatidylinositol phosphatase LOC106438251 /AT4G17910 87.25 transferases, transferring acyl groups LOC106441905 /AT1G10720 24.49 BSD domain-containing...”
- Molecular mechanisms of resistance to Myzus persicae conferred by the peach Rm2 gene: A multi-omics view
Le, Frontiers in plant science 2022 - “...1.8 8.40E-12 PYD2; Dihydropyrimidinase Prupe_8G070200 AT5G64370 84 Yes 2.0 1.30E-08 PYD3; Beta-ureidopropionase Shikimate pathway Prupe_2G143700 AT2G45300 77 Yes -1.8 2.50E-07 EPSPS; 3-phosphoshikimate 1-carboxyvinyltransferase Prupe_1G393400 AT5G10870 67 Yes 2.2 4.30E-09 CM2; Chorismate mutase 2 Prupe_1G281400 AT1G69370 66 Yes -3.2 8.40E-23 CM3; Chorismate mutase 3 Prupe_6G119200 AT1G08250 80...”
- Deciphering the structure of Arabidopsis thaliana 5-enol-pyruvyl-shikimate-3-phosphate synthase: An essential step toward the discovery of novel inhibitors to supersede glyphosate
Ruszkowski, Computational and structural biotechnology journal 2022 - “...Plant Mini Kit (Qiagen). The construct for the overexpression of AtEPSPS (Uniprot ID: P05466; locus At2g45300) was designed based on a comparative analysis of homologous sequences from plants and prediction of signal peptides using the TargetP 1.1 server [58] , [59] . The final polypeptide started...”
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BCc_191 3-phosphoshikimate 1-carboxyvinyltransferase from Buchnera aphidicola str. Cc (Cinara cedri)
24% identity, 94% coverage
C9EGX2 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Rahnella aquatilis (see paper)
28% identity, 96% coverage
BMA0430 prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase from Burkholderia mallei ATCC 23344
30% identity, 61% coverage
AROA_PETHY / P11043 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EC 2.5.1.19 from Petunia hybrida (Petunia) (see 5 papers)
29% identity, 81% coverage
- function: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate (PubMed:1939260). Involved in the accumulation of volatile benzoides in flowers, scent attracting pollinators (e.g. the night- active hawkmoth pollinator Manduca sexta) (PubMed:15805488).
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256) - First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...Brassica napus (P17688), Petunia hybrida (P11043), Solanum lycopersicum (P10748), Bordetella pertussis (P12421), Aeromonas salmonicida (Q03321), Rahnella...”
- A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity
Peng, PloS one 2012 - “...from E. coli (P07638), Aeromonas salmonicida (Q03321), Arabidopsis thaliana (P05466), N. tabacum (P23981), Petunia hybrida (P11043), Z. mays (CAA44974), and Bordetella pertussis (P12421). Class II AroA proteins were obtained from Pseudomonas strain PG2982 (P56952), A. tumefaciens strain CP4 (Q9R4E4), Bacillus subtilis (P20691), S. aureus (Q05615), and...”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...tabacum (Swiss-Prot P23981), Petunia hybrida (Swiss-Prot P11043), Zea mays (GenBank accession no. CAA44974), and Bordetella pertussis (Swiss-Prot P12421);...”
- Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Sun, Applied and environmental microbiology 2005 - “...thaliana (P05466), Nicotiana tabacum (P23981), Petunia hybrida (P11043), Zea mays (CAA44974), and Bordetella pertussis (P12421). Class II AroA proteins shown...”
Cbei_4575 3-phosphoshikimate 1-carboxyvinyltransferase from Clostridium beijerincki NCIMB 8052
26% identity, 97% coverage
M1CGC9 3-phosphoshikimate 1-carboxyvinyltransferase from Solanum tuberosum
28% identity, 81% coverage
- Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease
Xiao, BMC genomics 2020 - “...0.00 K7VPA4 Ribosomal protein L24 0.70 0.00 Q2XPW4 60S ribosomal protein L7A-like protein 0.69 0.00 M1CGC9 3-phosphoshikimate 1-carboxyvinyltransferase 0.78 0.00 M1BTT7 Ketol-acid reductoisomerase 0.78 0.00 M1AIT2 D-3-phosphoglycerate dehydrogenase 0.79 0.00 M1CZC0 ERBB-3 BINDING PR 0.81 0.00 A0A0M4KNM3 Carotenoid 9,10(9,10)-cleavage dioxygenase 1-like protein (Fragment) 0.77 0.00 Q9ZRB6...”
- “...suppression of proteins related to aromatic amino acid biosynthesis and metabolisms such as (phosphoshikimate 1-carboxyvinyltransferase (M1CGC9), ketol-acid reductoisomerase (M1BTT7), and D-3-phosphoglycerate dehydrogenase (M1AIT2). The phosphoshikimate 1-carboxyvinyltransferase is a key enzyme of the shikimate pathway, involved in the biosynthesis of multiple aromatic compounds, including chorismite, phenylalanine, tyrosine,...”
B0B7T5 3-phosphoshikimate 1-carboxyvinyltransferase from Chlamydia trachomatis serovar L2 (strain ATCC VR-902B / DSM 19102 / 434/Bu)
CTL0620 3-phosphoshikimate 1-carboxyvinyltransferase from Chlamydia trachomatis 434/Bu
29% identity, 93% coverage
Ot14g02430 RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta from Ostreococcus tauri
27% identity, 89% coverage
- Shikimate and phenylalanine biosynthesis in the green lineage
Tohge, Frontiers in plant science 2013 - “...SKL1 Sb08G018630 Zm01g26660 Bd2g03680 Os01g01302 SKL2 Mrcc02g03490 Ot07g01450 Sb01G027930 Zm01g22640 Bd3g34245 Os10g42700 ESPS Cr03g06830 Mrcc13g01100 Ot14g02430 Sb10G002230 Zm09g05500 Bd1g51660 Os06g04280 Osi06g03190 CS Cr01g12390 Mrcc05g01430 Ot02g06020 Sb01G040790 Zm01g10020 Bd1g67790 Os03g14990 Osi03g13340 Zm09g24540 CM Cr03g01600 Mrcc08g05060 Ot08g02860 Sb03G035460 Zm03g31000 Bd2g50800 Os01g55870 Osi01g52850 Sb04G005480 Zm05g21270 Bd3g06050 Os02g08410 Osi02g08160 Zm08g34320...”
P10748 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic from Solanum lycopersicum
28% identity, 81% coverage
- Dissemination of Linezolid Resistance Through Sex Pheromone Plasmid Transfer in Enterococcus faecalis.
Zou, Frontiers in microbiology 2020 - “...and proteomics ( Yan et al., 2018 ) analyses of a linezolid-resistant E. faecalis strain P10748 consistently revealed a significant co-upregulation of optrA gene with several genes involved in mating and pheromone response, implying a localization of this gene in a sex pheromone plasmid allowing highly...”
- “...faecalis clinical isolates, determine the whole plasmid genome sequence in the linezolid-resistant E. faecalis strain P10748, and identify mechanisms behind optrA transfer. Our results suggest, for the first time, that optrA -mediated linezolid resistance can be widely disseminated through sex pheromone plasmid transfer. Materials and Methods...”
- First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...Petunia hybrida (P11043), Solanum lycopersicum (P10748), Bordetella pertussis (P12421), Aeromonas salmonicida (Q03321), Rahnella aquatilis (C9EGX2), Escherichia...”
- Quantitative Proteomics Analysis of Membrane Proteins in Enterococcus faecalis With Low-Level Linezolid-Resistance.
Yan, Frontiers in microbiology 2018 - “...this study, we performed quantitative proteomics analysis of membrane proteins in an E. faecalis isolate (P10748) with low-level linezolid-resistance in comparison with two linezolid-susceptible strains 3138 and ATCC 29212, all of which have been previously investigated by whole transcriptome analysis. A total of 8,197 peptides associated...”
- “...P < 0.05. There were 14 significantly up-regulated and 6 significantly down-regulated proteins in strain P10748 compared to strains 3138 and ATCC 29212, which were in general positively correlated with transcription levels revealed in previous transcriptome studies. Our analysis suggests that the low-level linezolid-resistance in E....”
P23981 3-phosphoshikimate 1-carboxyvinyltransferase 1, chloroplastic from Nicotiana tabacum
28% identity, 81% coverage
- A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity
Peng, PloS one 2012 - “...proteins were obtained from E. coli (P07638), Aeromonas salmonicida (Q03321), Arabidopsis thaliana (P05466), N. tabacum (P23981), Petunia hybrida (P11043), Z. mays (CAA44974), and Bordetella pertussis (P12421). Class II AroA proteins were obtained from Pseudomonas strain PG2982 (P56952), A. tumefaciens strain CP4 (Q9R4E4), Bacillus subtilis (P20691), S....”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...thaliana (Swiss-Prot P05466), Nicotiana tabacum (Swiss-Prot P23981), Petunia hybrida (Swiss-Prot P11043), Zea mays (GenBank accession no. CAA44974), and...”
- Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Sun, Applied and environmental microbiology 2005 - “...(Q03321), Arabidopsis thaliana (P05466), Nicotiana tabacum (P23981), Petunia hybrida (P11043), Zea mays (CAA44974), and Bordetella pertussis (P12421). Class...”
YPO1390 3-phosphoshikimate 1-carboxyvinyltransferase from Yersinia pestis CO92
28% identity, 96% coverage
APJL_0697 3-phosphoshikimate 1-carboxyvinyltransferase from Actinobacillus pleuropneumoniae serovar 3 str. JL03
29% identity, 94% coverage
y2783 5-enolpyruvylshikimate-3-phosphate synthetase from Yersinia pestis KIM
28% identity, 96% coverage
aroA / CAH19218.1 3-phosphoshikimate 1-carboxyvinyltransferase from Pseudomonas putida (see paper)
Q683S1 3-phosphoshikimate 1-carboxyvinyltransferase from Pseudomonas putida
29% identity, 94% coverage
8umjA / A0A1D6NVZ6 Wild type epsp synthase complexed with glyphosate and shikimate-3- phosphate (see paper)
28% identity, 94% coverage
- Ligands: glyphosate; shikimate-3-phosphate (8umjA)
CT366 Phosphoshikimate 1-carboxyl vinyltransferase from Chlamydia trachomatis D/UW-3/CX
29% identity, 93% coverage
YPK_2670 3-phosphoshikimate 1-carboxyvinyltransferase from Yersinia pseudotuberculosis YPIII
28% identity, 96% coverage
- Fis Is Essential for Yersinia pseudotuberculosis Virulence and Protects against Reactive Oxygen Species Produced by Phagocytic Cells during Infection
Green, PLoS pathogens 2016 - “...numbers Accession numbers for the genes described in this study in NCBI are: aroA , YPK_2670; aroE , YPK_0321; purM , YPK_1253; rfaH , YPK_3937; wecC , YPK_4030; arnDT , YPK_1834-YPK_1835; dusB , YPK_0453; fis , YPK_0452; flgD , YPK_2423; psaEFABC , YPK_2761-YPK_2757; katG , YPK_3388;...”
- Identification of MrtAB, an ABC transporter specifically required for Yersinia pseudotuberculosis to colonize the mesenteric lymph nodes
Crimmins, PLoS pathogens 2012 - “...#2 aroB 1.29E-02 (4.48) 0.28 (2.62) YPK_0357 #1 and #2 purH 1.80E-02 (4.04) 0.66 (0.8) YPK_2670 #1 and #2 aroA 3.06E-02 (3.33) 0.44 (1.65) YPK_2047 #1 and #2 trpA 3.33E-02 (3.22) 0.61 (1.0) YPK_2528 #1 and #2 hisB 3.42E-02 (3.18) 0.87 (0.22) YPK_0356 #1 purD 9.53E-03...”
XP_008659331 3-phosphoshikimate 1-carboxyvinyltransferase 2 from Zea mays
28% identity, 83% coverage
K9PSG5 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Calothrix sp. (see paper)
28% identity, 93% coverage
U876_09965 3-phosphoshikimate 1-carboxyvinyltransferase from Aeromonas hydrophila NJ-35
28% identity, 96% coverage
LOC106438248 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic-like from Brassica napus
28% identity, 82% coverage
- Mapping of a novel clubroot disease resistance locus in Brassica napus and related functional identification
Jiang, Frontiers in plant science 2022 - “...family LOC106438246 AT2G45220 88.27 Plant invertase/pectin methylesterase inhibitor superfamily LOC106438247 AT2G45260 85.80 myosin-4 protein (DUF641) LOC106438248 AT2G45300 89.35 RNA 3-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta LOC106438249 AT2G45310 85.23 UDP-D-glucuronate 4-epimerase 4 BNAA03G56780D AT2G45320 87.82 polyphosphatidylinositol phosphatase LOC106438251 /AT4G17910 87.25 transferases, transferring acyl groups LOC106441905 /AT1G10720 24.49 BSD...”
A7Y7Y2 3-phosphoshikimate 1-carboxyvinyltransferase from Gossypium hirsutum
29% identity, 80% coverage
HI1589 3-phosphoshikimate-1-carboxyvinyltransferase (aroA) from Haemophilus influenzae Rd KW20
28% identity, 94% coverage
epsps-S / CAD01096.1 5-enolpyruvylshikimate-3-phosphate synthase, partial from Eleusine indica (see paper)
28% identity, 94% coverage
P17688 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic from Brassica napus
28% identity, 81% coverage
AT1G48860 3-phosphoshikimate 1-carboxyvinyltransferase, putative / 5-enolpyruvylshikimate-3-phosphate, putative / EPSP synthase, putative from Arabidopsis thaliana
Q9FVP6 3-phosphoshikimate 1-carboxyvinyltransferase from Arabidopsis thaliana
28% identity, 80% coverage
- Beneficial Effects of Phosphite in Arabidopsis thaliana Mediated by Activation of ABA, SA, and JA Biosynthesis and Signaling Pathways
Pérez-Zavala, Plants (Basel, Switzerland) 2024 - “...(AT5G66120), 3-dehydroquinate dehydratase/shikimate 5-dehydrogenase (MEE32/AT3G06350), shikimate kinase (SK1/AT2G21940 and SK2/AT4G39540), and 3-phosphoshikimate 1-carboxyvinyltransferase (EPSP/AT2G45300 and AT1G48860), to synthesize the SA precursor, chorismate, and isochorismate synthase (ICS1/AT1G74710, ICS2/AT1G8870) that produces isochorismate, which is the last enzymatic step required for SA synthesis. Up to 9 of the 11...”
- Identification and Expression Analysis of EPSPS and BAR Families in Cotton
Li, Plants (Basel, Switzerland) 2023 - “...In rice, LOCOs06g04280 is dominantly expressed in the root. In Arabidopsis , the expression of AT1G48860 in leaves was extremely significant. In tobacco, the accumulation of EPSPS transcripts was highest in mature leaves. After 14 days of herbicide stress, the expression of NtEPSPS was significantly upregulated,...”
- “...created using EPSPS II [ 53 ]. Arabidopsis has two natural EPSPS loci, AtEPSPS1 ( AT1G48860 ) and AtEPSPS2 ( AT2G45300 ), which are highly expressed throughout development [ 54 ]. It was found that overexpression of the natural gene encoding 5-enolpyruvate oxalate synthase (EPSP) may...”
- Transcriptomics and Metabolomics of Reactive Oxygen Species Modulation in Near-Null Magnetic Field-Induced Arabidopsis thaliana
Parmagnani, Biomolecules 2022 - “...h ( Figure 4 A), with the exception for DFR ( At5g42800 ), EPSPS ( At1g48860 ), CYP75B1 ( At5g07990 ), and a putative isoflavone reductase ( At1g75290 ), which upregulation-matched with the higher levels of dihydrogenistein-glucuronide ( 6 ), dihydroquercetin-glucoside ( 26 ), and dihydromyricetin-glucoside...”
- A Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis glyI4 Loss-of-Function Mutant
Proietti, Plants (Basel, Switzerland) 2021 - “...At3g53260), cinnamyl alcohol dehydrogenase 4 ( CAD4 , At3g19450), and 5-enolpyruvylshikimate-3-phosphate synthase ( EPSPS , At1g48860), in Col-8 and the glyI4 mutant. As shown in Figure 3 , the three genes were significantly downregulated in the glyI4 mutant compared to Col-8. It has frequently been observed...”
- “...(AGI) numbers of the studied genes are AT3g53260 ( PAL2 ), AT3g19450 ( CAD4 ), AT1g48860 ( EPSPS ), At4g05320 ( UBI10 ). Primers were the following: PAL2 (Fw: AGGCAGCGTTAAGGTTGAGT; Rv: GGTGACTCCGTAACTGTCAGTAC), CAD4 (Fw: GACACCATGATCGTCAATCAAAAG; Rv: TCAACGGACTATAAACCGTTACTC), EPSPS (Fw: TGCTAAATGGTTCTGAGATTCGTC; Rv: AGACCCGAGATTTCTCTAAT GGG), UBI10 (Fw: AAAGAGATAACAGGAACGGAAACATAGT;...”
- Insights into the Role of Transcriptional Gene Silencing in Response to Herbicide-Treatments in Arabidopsis thaliana
Markus, International journal of molecular sciences 2021 - “...their impact on RdDM and TGS regulation. Glyphosate is an inhibitor of 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE (EPSPS; AT1G48860); imazethapyr belongs to inhibitors of ACETOLACTATE SYNTHASE (ALS; AT3G48560), and finally, 2,4-D (2,4-dichlorophenoxyacetic acid) is a synthetic auxin, which mimics naturally the plant hormone indole-3-acetic acid (IAA), the principal natural...”
- Towards novel herbicide modes of action by inhibiting lysine biosynthesis in plants
Soares, eLife 2021 - “...Moreover, upon comparison to the known glyphosate herbicide target, 5- enol pyruvyl-shikimate 3-phosphate synthase ( At1G48860 ), expression of DHDPS1 is considerably lower at almost all developmental stages, while expression of DHDPS2 is comparable at all stages, except in the dry seeds ( Klepikova et al.,...”
- Insights into the function of NADPH thioredoxin reductase C (NTRC) based on identification of NTRC-interacting proteins in vivo
González, Journal of experimental botany 2019 - “...9 (9) Q9SK84 At1g22410 Class-II DAHP synthase-like protein C 2 6.45 7 (7) x Q9FVP6 At1g48860 EPSPS, 5-Enolpyruvylshikimate-3-phosphate synthase C 2 14.77 10 (9) D7MUW5 At5g54810 TRPB, Tryptophan synthase beta-subunit C, M 2 5.89 5 (5) x Q9LU63 At5g51110 PDL1, PCD/DCoH-like protein (4-alpha-hydroxytetrahydrobiopterin dehydratase activity) C...”
- Evaluation of glyphosate resistance in Arabidopsis thaliana expressing an altered target site EPSPS
Sammons, Pest management science 2018 - “...2 MATERIALS AND METHODS 2.1 Cloning Arabidopsis thaliana contains two 5enolpyruvylshikimate3phosphate (EPSP) synthases: At 1EPSPS (AT1G48860) and At 2EPSPS (AT2G45300) located on chromosomes 1 and 2, respectively, both expressing EPSPS with highly similar mature protein primary amino acid sequence. Using the nucleotide sequence information in the...”
- More
- Insights into the function of NADPH thioredoxin reductase C (NTRC) based on identification of NTRC-interacting proteins in vivo
González, Journal of experimental botany 2019 - “...2.4 9 (9) Q9SK84 At1g22410 Class-II DAHP synthase-like protein C 2 6.45 7 (7) x Q9FVP6 At1g48860 EPSPS, 5-Enolpyruvylshikimate-3-phosphate synthase C 2 14.77 10 (9) D7MUW5 At5g54810 TRPB, Tryptophan synthase beta-subunit C, M 2 5.89 5 (5) x Q9LU63 At5g51110 PDL1, PCD/DCoH-like protein (4-alpha-hydroxytetrahydrobiopterin dehydratase activity)...”
CPSIT_0767 3-phosphoshikimate 1-carboxyvinyltransferase from Chlamydia psittaci 6BC
29% identity, 96% coverage
Q3MAV9 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Nostoc sp. (see paper)
27% identity, 93% coverage
AroA / b0908 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Escherichia coli K-12 substr. MG1655 (see 12 papers)
aroA / P0A6D3 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Escherichia coli (strain K12) (see 14 papers)
AROA_ECOLI / P0A6D3 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Escherichia coli (strain K12) (see 9 papers)
P0A6D3 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Escherichia coli (see 12 papers)
2aa9A / P0A6D3 Epsp synthase liganded with shikimate (see paper)
b0908 3-phosphoshikimate 1-carboxyvinyltransferase from Escherichia coli str. K-12 substr. MG1655
NP_415428 3-phosphoshikimate 1-carboxyvinyltransferase from Escherichia coli str. K-12 substr. MG1655
ECs0991 5-enolpyruvylshikimate-3-phosphate synthetase from Escherichia coli O157:H7 str. Sakai
29% identity, 96% coverage
- function: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256)
subunit: Monomer. - Ligand: (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid (2aa9A)
- Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions
Orth, BMC systems biology 2012 - “...(F) IV dmlA (b1800) 6.00E-26 icd (b1136) 1.00E-26 leuB (b0073) 2.00E-15 R00732 (R) III aroA (b0908) 5.00E-32 murA (b3189) 7.00E-8 R00733 (R) III tyrA (b2600) 2.80E-2 R01393 (R) I global orphan R01618 (R) IV glgP (b3428) 2.10 R01713 (F) I global orphan R01731 (F) IV tyrB...”
- A PhoQ/P-regulated small RNA regulates sensitivity of Escherichia coli to antimicrobial peptides
Moon, Molecular microbiology 2009 - “...2.2 putative ATP-dependent RNA helicase ycaD b0898 2.6 1.3 2.2 2.0 Putative MFS transporter aroA b0908 3.0 2.0 2.0 1.3 5-enolpyruvylshikimate-3-phosphate synthetase ndh b1109 2.6 1.7 5.2 3.6 respiratory NADH dehydrogenase trpH b1266 2.1 2.1 2.1 1.0 putative enzymes ydgI b1605 2.2 1.2 3.1 2.8 putative...”
- Genome-scale analysis to the impact of gene deletion on the metabolism of E. coli: constraint-based simulation approach
Xu, BMC bioinformatics 2009 - “...b4006, b4177 s0001 b4244, b4245 SS NSP CAC GSM TTPM TIM genes b0639 b0720 b0907 b0908, b1260 b0914 b1098 b1136 b1261, b1262 s0001 b2827 b1263, b1264 b3648 b1693, b2329 s0001 b2599, b2600 b3389 SS AAM FM ACM HM CM genes b0928 b1415 b1415 b2019, b2020 b2750,...”
- Experimental and computational assessment of conditionally essential genes in Escherichia coli
Joyce, Journal of bacteriology 2006 - “...argA (b2818) argE (b3957) argH (b3960) aroA (b0908) aroB (b3389) aroC (b2329) aroD (b1693) aroE (b3281) cysC (b2750) cysD (b2752) cysE...”
- Combined, functional genomic-biochemical approach to intermediary metabolism: interaction of acivicin, a glutamine amidotransferase inhibitor, with Escherichia coli K-12
Smulski, Journal of bacteriology 2001 - “...flgD b3256 b4015 b4016 b1623 b0111 b0050 b0564 b0469 b0908 b2601 b1704 b3433 b0930 b1597 b3734 b3731 b3732 b3736 b3572 b0778 b1270 b1661 b2155 b3556 b1823 b1418...”
- A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
Cao, PloS one 2012 - GeneRIF: A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
- Acid-induced formation of molten globule states in the wild type Escherichia coli 5-enolpyruvylshikimate 3-phosphate synthase and its three mutated forms: G96A, A183T and G96A/A183T.
Haghani, The protein journal 2011 (PubMed)- GeneRIF: The results suggest that there is a direct relationship between stability of 5-enolpyruvylshikimate 3-phosphate synthase and pH stability of its folding intermediates
- Structural basis of glyphosate resistance resulting from the double mutation Thr97 -> Ile and Pro101 -> Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli.
Funke, The Journal of biological chemistry 2009 - GeneRIF: analysis of glyphosate resistance resulting from the double mutation Thr97 -> Ile and Pro101 -> Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli
- Catalytic residues and an electrostatic sandwich that promote enolpyruvyl shikimate 3-phosphate synthase (AroA) catalysis.
Berti, Biochemistry 2009 (PubMed)- GeneRIF: In an active site that is overwhelmingly positively charged, Asp-313 and Glu-341 side chains of aroA form an "electrostatic sandwich" around the positive charge at C2 in cationic intermediates/transition states, stabilizing them and promoting catalysis.
- A novel RPMXR motif among class II 5-enolpyruvylshikimate-3-phosphate synthases is required for enzymatic activity and glyphosate resistance.
Li, Journal of biotechnology 2009 (PubMed)- GeneRIF: We identified a novel RPMXR motif that is strictly conserved among class II EPSP synthases and is required for enzymatic acitvity and glyphosate resistance.
- Structural basis of glyphosate tolerance resulting from mutations of Pro101 in Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthase.
Healy-Fried, The Journal of biological chemistry 2007 (PubMed)- GeneRIF: glyphosate ternary complexes of P101S and P101L 5-enolpyruvylshikimate-3-phosphate synthase were determined to between 1.5- and 1.6-A resolution
- Protein language models are performant in structure-free virtual screening
Lam, Briefings in bioinformatics 2024 - “...[ 66 ]. For BIND, a batch size of one was used and the sequence P0A6D3 obtained from UniProt, and the ESM-2 embeddings compute time not included in the speed benchmark as only one embedding has to be calculated per protein, and can be reused for...”
- Genome-Guided Analysis of Seven Weed Species Reveals Conserved Sequence and Structural Features of Key Gene Targets for Herbicide Development
Shah, Frontiers in plant science 2022 - “...PDB 1YBH) from Arabidopsis thaliana . For EPSP synthase, the protein from Escherichia coli (UniProt P0A6D3; PDB 1G6S) was used; no protein structures for plant EPSP synthase have been resolved. For each set of protein sequences, multiple sequence alignment was performed using Cluster Omega ( Sievers...”
- Deciphering the structure of Arabidopsis thaliana 5-enol-pyruvyl-shikimate-3-phosphate synthase: An essential step toward the discovery of novel inhibitors to supersede glyphosate
Ruszkowski, Computational and structural biotechnology journal 2022 - “...mapped by a superposition with the structure of EPSPS from E. coli (EcEPSPS, Uniprot ID: P0A6D3) in complex with S3P and glyphosate (PDB ID: 1g6s [45] , Fig. 4 ). Due to the different state (open vs closed), individual domains of AtEPSPS were used to reveal...”
- Knowns and Unknowns of Vitamin B6 Metabolism in Escherichia coli
Tramonti, EcoSal Plus 2021 (secret) - First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...Rahnella aquatilis (C9EGX2), Escherichia coli (P0A6D3), Salmonella typhimurium (P07637), Pseudomonas fluorescens (A1Z0H6), Pseudomonas putida (Q683S1),...”
- Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate
Döring, Biosensors 2019 - “...The ORF of aroA coding for Escherichia (E.) coli EPSPS (EcEPSPS, gene ID: 945528, UniProt: P0A6D3) was PCR-amplified from E. coli DH10 (New England Biolabs GmbH, Frankfurt am Main, Germany). To create the fusion gene Ccg2_GS_EcEPSPS a glycine-serine linker sequence (G 4 S) 3 was adhered...”
- An optimized transit peptide for effective targeting of diverse foreign proteins into chloroplasts in rice
Shen, Scientific reports 2017 - “...OsOXO was predicted by SWISS-MODEL ( http://swissmodel.expasy.org/ ). The tertiary structure of EPSP (UniProt ID: P0A6D3), NPT-II (UniProt ID: P00552), cry1Ac (UniProt ID: P05068), EcKAT (UniProt ID: P21179), EcGCL (UniProt ID: P0AEP7), OsrbcS (UniProt ID: Q01NY7) and the 80 precursor proteins were obtained from the RCSB...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...TABLE 2--Continued pI/MW Accession no.b AroA P0A6D3 3-Phosphoshikimate 1carboxyvinyltransferase 5.37/46,095.78 AroC AroD AroF P12008 P05194 P00888 Chorismate...”
- Global transcriptional response of Escherichia coli O157:H7 to growth transitions in glucose minimal medium
Bergholz, BMC microbiology 2007 - “...ycaP 2.56 2 ECs0248 fadE 4.57 2 ECs0990 serC -3.29 1 ECs0253 dinJ 2.46 2 ECs0991 aroA -4.24 1 ECs0269 proB -2.11 1 ECs1007 mukB -2.12 1 ECs0383 yahO 4.53 2 ECs1013 asnS -2.74 1 ECs0384 prpR 3.63 2 ECs1014 pncB -2.37 1 ECs0390 codA -2.78...”
t1956 3-phosphoshikimate 1-carboxyvinyltransferase from Salmonella enterica subsp. enterica serovar Typhi Ty2
29% identity, 96% coverage
NP_459953 3-enolpyruvylshikimate-5-phosphate synthetase from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
P07637 3-phosphoshikimate 1-carboxyvinyltransferase from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
STM0978 3-enolpyruvylshikimate-5-phosphate synthetase from Salmonella typhimurium LT2
STM14_1106 3-phosphoshikimate 1-carboxyvinyltransferase from Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
29% identity, 96% coverage
- aroA-Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant.
Felgner, mBio 2016 - GeneRIF: DeltaaroA mutants display pleiotropic alterations in cellular physiology and lipid and amino acid metabolism, as well as increased sensitivity to penicillin, complement, and phagocytic uptake.
- First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...Escherichia coli (P0A6D3), Salmonella typhimurium (P07637), Pseudomonas fluorescens (A1Z0H6), Pseudomonas putida (Q683S1), Janibacter limosus (L7PA93). Class...”
- Persistence of vaccine origin Salmonella Typhimurium through the poultry production continuum, and development of a rapid typing scheme for their differentiation from wild type field isolates
Johnson, Poultry science 2024 - “...ST strain, the aroA gene was disrupted at position 1,062,727 bp in the LT2 genome (STM0978). In the Megan Vac 1 and Megan Egg strains, the crp gene was disrupted at position 3,615,908 bp in the LT2 genome (STM3466), and the cyaA gene was disrupted at...”
- Inorganic Polyphosphate Is Essential for Salmonella Typhimurium Virulence and Survival in Dictyostelium discoideum
Varas, Frontiers in cellular and infection microbiology 2018 - “...pgm Phosphoglucomutase STM14_1013 STM0863 dacC D-alanyl-D-alanine carboxypeptidase fraction C STM14_1056 STM0939 ybjD Hypothetical protein STM14_1106 STM0978 aroA 3-phosphoshikimate 1-carboxyvinyltransferase STM14_1259 STM1109 Putative periplasmic protein STM14_1309 STM1143 csgB Curlin minor subunit STM14_1432 Phage replication protein O STM14_1515 STM1255 Putative ABC transporter periplasmic binding protein STM14_1565 STM1290 gapA...”
- “...STM14_0992 STM0798 uvrB Excinuclease ABC subunit B STM14_1013 STM0863 dacC D-alanyl-D-alanine carboxypeptidase fraction C STM14_1106 STM0978 aroA 3-phosphoshikimate 1-carboxyvinyltransferase STM14_1353 STM1182 flgJ Peptidoglycan hydrolase STM14_1459/STM14_3188 STM2605 Prophage head-tail preconnector STM14_1515 STM1255 Putative ABC transporter periplasmic binding protein STM14_1565 STM1290 gapA Glyceraldehyde-3-phosphate dehydrogenase STM14_1723 STM1426 ribE Riboflavin...”
- Subtyping of Salmonella enterica Subspecies I Using Single-Nucleotide Polymorphisms in Adenylate Cyclase
Guard, Foodborne pathogens and disease 2016 - “...14-Sep DNA polymerization 4 pgk STM3069 1164 394 33 4 0.121 15-Sep Glycolysis 5 aroA STM0978 1284 441 46 37 0.804 14-Sep Tryptophan biosynthesis 6 manB STM2104 1371 411 66 60 0.909 14-Sep Colanic acid synthesis 7 atpD STM3865 1383 385 31 3 0.097 15-Sep ATP...”
- Identification of Novel Genes Mediating Survival of Salmonella on Low-Moisture Foods via Transposon Sequencing Analysis
Jayeola, Frontiers in microbiology 2020 - “...2.18 1.59 1.86 1.08 3.77 Others STM14_0896 ( gpmA ) 1.97 NC 2.90 NC NC STM14_1106 ( aroA ) 1.24 1.02 NC NC NC STM14_1370 ( fabF ) 1.55 NC 1.39 NC NC STM14_2056 ( rnb ) 1.94 1.41 ND*** 1.82 ND STM14_2341 ( flhD )...”
- Inorganic Polyphosphate Is Essential for Salmonella Typhimurium Virulence and Survival in Dictyostelium discoideum
Varas, Frontiers in cellular and infection microbiology 2018 - “...STM0698 pgm Phosphoglucomutase STM14_1013 STM0863 dacC D-alanyl-D-alanine carboxypeptidase fraction C STM14_1056 STM0939 ybjD Hypothetical protein STM14_1106 STM0978 aroA 3-phosphoshikimate 1-carboxyvinyltransferase STM14_1259 STM1109 Putative periplasmic protein STM14_1309 STM1143 csgB Curlin minor subunit STM14_1432 Phage replication protein O STM14_1515 STM1255 Putative ABC transporter periplasmic binding protein STM14_1565 STM1290...”
- “...TolB STM14_0992 STM0798 uvrB Excinuclease ABC subunit B STM14_1013 STM0863 dacC D-alanyl-D-alanine carboxypeptidase fraction C STM14_1106 STM0978 aroA 3-phosphoshikimate 1-carboxyvinyltransferase STM14_1353 STM1182 flgJ Peptidoglycan hydrolase STM14_1459/STM14_3188 STM2605 Prophage head-tail preconnector STM14_1515 STM1255 Putative ABC transporter periplasmic binding protein STM14_1565 STM1290 gapA Glyceraldehyde-3-phosphate dehydrogenase STM14_1723 STM1426 ribE...”
7tm5B / A0A0H3GV01 Crystal structure of shikimate-3-phosphate bound 3-phosphoshikimate 1- carboxyvinyltransferase from klebsiella pneumoniae
29% identity, 96% coverage
- Ligand: shikimate-3-phosphate (7tm5B)
B2IYE4 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Nostoc punctiforme (see paper)
27% identity, 96% coverage
TWT567 3-phosphoshikimate 1-carboxyvinyltransferase from Tropheryma whipplei str. Twist
28% identity, 87% coverage
epsps-R / CAD01095.1 5-enolpyruvylshikimate-3-phosphate synthase, partial from Eleusine indica (see paper)
27% identity, 94% coverage
CCA00723 3-phosphoshikimate 1-carboxyvinyltransferase from Chlamydophila caviae GPIC
30% identity, 96% coverage
A2T9R8 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Dunaliella salina (see paper)
28% identity, 82% coverage
Q93VK6 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Oryza sativa (see paper)
27% identity, 82% coverage
AROA_KLEPN / P24497 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Klebsiella pneumoniae (see paper)
29% identity, 96% coverage
- function: Target of the herbicide glyphosate. Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3- phosphate and inorganic phosphate.
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256)
subunit: Monomer.
Q8YMB5 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Nostoc sp. (see paper)
all5019 3-phosphoshikimate 1-carboxyvinyltransferase from Nostoc sp. PCC 7120
26% identity, 93% coverage
- Tradeoffs between phage resistance and nitrogen fixation drive the evolution of genes essential for cyanobacterial heterocyst functionality
Kolan, The ISME journal 2024 - “...56 ] that was observed previously. One of these substrains (RE1) had a mutation in all5019, a gene that encodes for 3-phosphoshikimate 1-carboxyvinyltransferase, which is involved in aromatic amino acids biosynthesis. The other substrain (RE3) has a mutation located upstream to all3346, which encodes for a...”
- “...these genes take part of is yet unknown, the Mch phenotype caused by mutations in all5019 and upstream of all3346 suggests that they are potential regulators of heterocyst differentiation. A similar phenotype was observed in three substrains (RB1, RB8, and RB20) carrying a single mutation in...”
B5XY87 3-phosphoshikimate 1-carboxyvinyltransferase from Klebsiella pneumoniae (strain 342)
28% identity, 96% coverage
Q9RVD3 3-phosphoshikimate 1-carboxyvinyltransferase from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
30% identity, 94% coverage
STH1419 3-phosphoshikimate-1-carboxyvinyltransferase from Symbiobacterium thermophilum IAM 14863
30% identity, 93% coverage
PSPTO1748 prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase family protein from Pseudomonas syringae pv. tomato str. DC3000
35% identity, 41% coverage
DVU0463 3-phosphoshikimate 1-carboxyvinyltransferase from Desulfovibrio vulgaris Hildenborough
30% identity, 93% coverage
ARO1 / P08566 pentafunctional AROM polypeptide (EC 4.2.3.4; EC 1.1.1.25; EC 2.7.1.71; EC 2.5.1.19; EC 4.2.1.10) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 3 papers)
ARO1_YEAST / P08566 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
NP_010412 pentafunctional protein ARO1p from Saccharomyces cerevisiae S288C
YDR127W Aro1p from Saccharomyces cerevisiae
28% identity, 25% coverage
- function: The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis
catalytic activity: 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3- dehydroquinate + phosphate (RHEA:21968)
catalytic activity: 3-dehydroquinate = 3-dehydroshikimate + H2O (RHEA:21096)
catalytic activity: shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+) (RHEA:17737)
catalytic activity: shikimate + ATP = 3-phosphoshikimate + ADP + H(+) (RHEA:13121)
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256)
cofactor: Zn(2+) (Binds 2 Zn(2+) ions per subunit.)
subunit: Homodimer. - Histoplasma capsulatum Relies on Tryptophan Biosynthesis To Proliferate within the Macrophage Phagosome
Shen, Infection and immunity 2023 (secret) - Genetic variation in Dip5, an amino acid permease, and Pdr5, a multiple drug transporter, regulates glyphosate resistance in S. cerevisiae.
Rong-Mullins, PloS one 2017 - GeneRIF: Sequence comparison between the target proteins, i.e., the plant EPSPS and the yeast orthologous protein Aro1, predicted that yeast would be resistant to glyphosate. However, the growth variation seen in the subset of yeast tested was not due to polymorphisms within Aro1, instead, it was caused by genetic variation in an ABC multiple drug transporter, Pdr5, and an amino acid permease, Dip5
- Protein-protein interaction analysis for functional characterization of helicases.
Zybailov, Methods (San Diego, Calif.) 2016 - “...25 866 9.6E-18 P53297 PAB1-binding protein 1 0.1 54 1851 1.5E-36 0.1 25 866 9.6E-18 P08566 Pentafunctional AROM polypeptide 4 47 40 1.3E-26 4 30 26 2.4E-16 P32445 Single-stranded DNA-binding protein RIM1 5 58 40 2.2E-32 5 29 20 5.8E-15 P25367 [PIN+] prion protein RNQ1 3...”
- Analysis of Protein-protein Interaction Interface between Yeast Mitochondrial Proteins Rim1 and Pif1 Using Chemical Cross-linking Mass Spectrometry.
Zybailov, Journal of proteomics & bioinformatics 2015 - “...25 866 9.6E-18 P53297 PAB1-binding protein 1 0.1 54 1851 1.5E-36 0.1 25 866 9.6E-18 P08566 Pentafunctional AROM polypeptide 4 47 40 1.3E-26 4 30 26 2.4E-16 P32445 Single-stranded DNA-binding protein RIM1 5 58 40 2.2E-32 5 29 20 5.8E-15 P25367 [PIN+] prion protein RNQ1 3...”
- Multiple horizontal gene transfer events and domain fusions have created novel regulatory and metabolic networks in the oomycete genome
Morris, PloS one 2009 - “...bisphosphatase Q9MB58 Q16877 P32604 Ps143339 amino acid biosynthesis Pentafunctional aromatic amino acid synthesis None None P08566 Ps155429 Aspartate biosynthesis Aspartate kinase-homoserine dehydrogenase Q9SA18 None P10869 Formation of some novel MF proteins preceded the split between diatoms and oomycetes To determine if the formation of MF proteins...”
- Genome bias influences amino acid choices: analysis of amino acid substitution and re-compilation of substitution matrices exclusive to an AT-biased genome
Paila, Nucleic acids research 2008 - “...of our matrix. A local alignment was generated for PFB0280w and the yeast AROM complex, P08566; known to have the EPSP and SK activities. The signature motifs for EPSP and SK were obtained for P08566, identical to the PROSITE pattern and for PFB0280w with a mismatch...”
- “...The sequences compared here are the P. falciparum hypothetical protein, PFB0280w and yeast multifunctional protein, P08566. ( a ) The alignment yielded with BLOSUM100, showing no alignment overlap for the motif regions, EPSP synthase I and shikimate kinase. ( b ) The alignment with BLOSUM50 showing...”
- Differential requirements for Gcn5 and NuA4 HAT activities in the starvation-induced versus basal transcriptomes
Zheng, Nucleic acids research 2023 - “...YBR218C YBR248C YBR249C YCL030C YCR005C YDL131W YDL182W YDR127W YDR354W YDR379C-A YDR380W YER052C YER055C YER069W YER175C YFL026W YGL032C YGL117W YGL186C...”
- “...YBR249C YCL030C YCR005C YDL025C YDL131W YDL182W YDL198C YDR127W YDR354W YDR379C-A YDR380W YER052C YER053C YER055C YER175C YFL014W YFL026W YGL032C YGL117W...”
- A novel ER membrane protein Ehg1/May24 plays a critical role in maintaining multiple nutrient permeases in yeast under high-pressure perturbation
Kurosaka, Scientific reports 2019 - “...0.90.1 1.20.4 1.20.5 TRP1 YDR007W 4.00.0 0.20.1 0.40.0 3.40.2 0.20.0 0.30.0 0.80.1 1.10.4 0.70.1 ARO1 YDR127W 3.80.2 0.10.0 0.50.0 2.40.2 0.20.0 0.30.1 0.60.0 1.10.1 0.80.2 TRP4 YDR354W 4.40.1 0.40.1 0.90.4 4.00.3 0.30.1 0.80.3 0.90.1 0.70.2 0.90.1 TRP5 YGL026C 4.30.2 0.40.1 0.50.2 4.00.2 0.30.0 0.50.2 0.90.1 0.70.1...”
- Yeast screen for modifiers of C9orf72 poly(glycine-arginine) dipeptide repeat toxicity
Chai, FEMS yeast research 2018 - “...and other molecular biosynthetic pathways (10/133) alt1 YLR089C YDR127W cho2 YGR157W dph6 elo3 YLR143W YLR372W ilv1 YER086W ino1 YJL153C ipk1 YDR315C met2...”
- Characterizing meiotic chromosomes' structure and pairing using a designer sequence optimized for Hi-C
Muller, Molecular systems biology 2018 - “...and B). Sequencing confirmed that 140kb within the targeted region, encompassing open reading frames (ORFs) YDR127w to YDR196c, was replaced by the redesigned sequence and that all the mutations were introduced at the correct positions corresponding to a total of ~2% divergence with the reference genomes...”
- RSC Chromatin-Remodeling Complex Is Important for Mitochondrial Function in Saccharomyces cerevisiae
Imamura, PloS one 2015 - “...AminoTransferase YJL071W ARG2 ARGinine requiring YJL088W ARG3 ARGinine requiring YER069W ARG5 , 6 ARGinine requiring YDR127W ARO1 AROmatic amino acid requiring YOR303W CPA1 Carbamyl Phosphate synthetase A YAL012W CYS3 CYStathionine gamma-lyase YEL046C GLY1 GLYcine requiring YDR158W HOM2 HOMoserine requiring YER052C HOM3 HOMoserine requiring YJR139C HOM6 HOMoserine...”
- Environmental systems biology of cold-tolerant phenotype in Saccharomyces species adapted to grow at different temperatures
Paget, Molecular ecology 2014 - “...Methionine metabolism 4.4.1.8 Cystathionine b lyase: peroxisomal YGL184w STR3 Methionine metabolism 4.4.1.8 3 dehydroquinate synthase YDR127w ARO1 Tyrosine tryptophan and phenylalanine metabolism 4.2.3.4 Fatty acid: CoA ligase: tetradecanoate YOR317w YIL009w YMR246w FAA1 FAA3 FAA4 Fatty acid biosynthesis 6.2.1.3 Fatty acid: CoA ligase: tetradecanoate: peroxisomal YER015w FAA2...”
- Human stefin B role in cell's response to misfolded proteins and autophagy
Polajnar, PloS one 2014 - “...- CSTB ). Systematic (standard) name of the gene Function of the corresponding protein R YDR127W (ARO1) Penta-functional aromatic protein, catalysing 5 steps in synthesis of aromatic amino acids 13.893 YLR324W (PEX30) Peroxisomal integral protein, involved in regulation of peroxisome number 12.558 YML048W (GSF2) Integral membrane...”
- The antifungal eugenol perturbs dual aromatic and branched-chain amino acid permeases in the cytoplasmic membrane of yeast
Darvishi, PloS one 2013 - “...Name Standard Name Average % colony size reduction Aromatic and branched-chain amino acid biosynthesis pathways YDR127W ARO1 95.1 YGL148W ARO2 97.6 YPR060C ARO7 91.6 YDR007W TRP1 93.1 YER086W ILV1 96.8 Transmembrane transport specifically amino acids transport YBR068C BAP2 94.3 YFL055W AGP3 71.2 YBR077C SLM4 90.7 YEL031W...”
- More
A1Z0H6 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Pseudomonas fluorescens (see paper)
29% identity, 96% coverage
BMA0235 3-phosphoshikimate-1-carboxyvinyltransferase, putative from Burkholderia mallei ATCC 23344
28% identity, 94% coverage
SCO5212 3-phosphoshikimate 1-carboxyvinyltransferase from Streptomyces coelicolor A3(2)
SLI_5501 3-phosphoshikimate 1-carboxyvinyltransferase from Streptomyces lividans 1326
29% identity, 97% coverage
- Phylogenomic Analysis of Natural Products Biosynthetic Gene Clusters Allows Discovery of Arseno-Organic Metabolites in Model Streptomycetes
Cruz-Morales, Genome biology and evolution 2016 - “...a major clade associated with central metabolism; this clade includes SLI_5501 from S. lividans and SCO5212 from S. coelicolor . More importantly, the phylogeny also includes a divergent clade with at least three sub-clades, two that include family members linked to the known BGCs of the...”
- Quantitative proteomics analysis of Streptomyces coelicolor development demonstrates that onset of secondary metabolism coincides with hypha differentiation
Manteca, Molecular & cellular proteomics : MCP 2010 - “...SCO3304 SCO2504 SCO3023 SCO5739 SCO1773 SCO1494 SCO5547 SCO4958 SCO0216 SCO5212 SCO2198 SCO1660 SCO3834 SCO5385 SCO1514 SCO3899 SCO3060 SCO4917 6 4 2 4 2 2 6 8...”
- Changes in the extracellular proteome caused by the absence of the bldA gene product, a developmentally significant tRNA, reveal a new target for the pleiotropic regulator AdpA in Streptomyces coelicolor
Kim, Journal of bacteriology 2005 - “...data for the abundance of 3-phosphoshikimate 1-carboxyvinyltransferase SCO5212 and the putative dehydrogenase SCO5262 in total protein extracts. Four proteins...”
- “...extracts, as do the putative 3-phosphoshikimate 1-carboxyvinyltransferase SCO5212 predicted to be involved in amino acid metabolism, the prolyl aminopeptidase...”
- Phylogenomic Analysis of Natural Products Biosynthetic Gene Clusters Allows Discovery of Arseno-Organic Metabolites in Model Streptomycetes
Cruz-Morales, Genome biology and evolution 2016 - “...actinobacterial AroA enzyme family shows a major clade associated with central metabolism; this clade includes SLI_5501 from S. lividans and SCO5212 from S. coelicolor . More importantly, the phylogeny also includes a divergent clade with at least three sub-clades, two that include family members linked to...”
XP_722769 Aro1p from Candida albicans SC5314
30% identity, 27% coverage
Ddes_0335 3-phosphoshikimate 1-carboxyvinyltransferase from Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774
26% identity, 86% coverage
- Coordinated response of the Desulfovibrio desulfuricans 27774 transcriptome to nitrate, nitrite and nitric oxide
Cadby, Scientific reports 2017 - “...0.00129 Ddes_0333 Major facilitator family membrane transport protein 1.79 0.00336 Ddes_0334 Prephenate dehydrogenase 1.85 0.00164 Ddes_0335 3-phosphoshikimate 1-carboxyvinyltransferase 2.29 4.16e-5 Ddes_0336 Chorismate mutase 2.47 3.53e-6 Ddes_0337 3-dehydroquinate synthase 1.87 0.00292 Ddes_0525 4Fe-4S ferredoxin family 1.94 0.00030 Ddes_0526 Pyridoxamine 5-phosphate oxidase-related FMN-binding 2.6 1.67e-6 Ddes_0527 Flavodoxin family...”
- “...1.59E-06 R Ddes_0290 alaS Alanyl-tRNA synthetase 1.49 0.0132 J Ddes_0334 Prephenate dehydrogenase 1.52 0.0206 E Ddes_0335 3-phosphoshikimate 1-carboxyvinyltransferase 1.35 0.0377 E Ddes_0336 Chorismate mutase 1.56 0.00875 E Ddes_0337 3-dehydroquinate synthase 1.45 0.0119 E Ddes_0338 Fructose-bisphosphate aldolase 1.34 0.0334 G Ddes_0339 Pyridoxal phosphate-dependent D-cysteine desulfhydrase family 1.67...”
DvMF_1748 3-phosphoshikimate 1-carboxyvinyltransferase from Desulfovibrio vulgaris str. Miyazaki F
28% identity, 92% coverage
P12421 3-phosphoshikimate 1-carboxyvinyltransferase from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
27% identity, 94% coverage
- First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...Solanum lycopersicum (P10748), Bordetella pertussis (P12421), Aeromonas salmonicida (Q03321), Rahnella aquatilis (C9EGX2), Escherichia coli (P0A6D3), Salmonella...”
- A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity
Peng, PloS one 2012 - “...Arabidopsis thaliana (P05466), N. tabacum (P23981), Petunia hybrida (P11043), Z. mays (CAA44974), and Bordetella pertussis (P12421). Class II AroA proteins were obtained from Pseudomonas strain PG2982 (P56952), A. tumefaciens strain CP4 (Q9R4E4), Bacillus subtilis (P20691), S. aureus (Q05615), and S. pneumoniae (Q9S400). Growth of cells in...”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...accession no. CAA44974), and Bordetella pertussis (Swiss-Prot P12421); class II AroA proteins shown are from Pseudomonas sp. strain PG2982 (Swiss-Prot P56952),...”
- Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Sun, Applied and environmental microbiology 2005 - “...(P11043), Zea mays (CAA44974), and Bordetella pertussis (P12421). Class II AroA proteins shown are from Pseudomonas sp. strain PG2982 (P56952), Agrobacterium...”
PAAG_07102 pentafunctional AROM polypeptide from Paracoccidioides lutzii Pb01
29% identity, 26% coverage
- Transcriptional and proteomic responses to carbon starvation in Paracoccidioides
Lima, PLoS neglected tropical diseases 2014 - “...* cysteine biosynthesis PAAG_07089 homocitrate synthase * lysine biosynthesis PAAG_04348 homoserine kinase * threonine biosynthesis PAAG_07102 pentafunctional AROM polypeptide * aromatic group biosynthesis PAAG_05929 sulfate adenylyltransferase * cysteine and methionine biosynthesis C-compound and carbohydrate metabolism PAAG_02975 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase * phenylacetate catabolic process PAAG_02769 pyruvate dehydrogenase...”
cg0873 3-phosphoshikimate 1-carboxyvinyltransferase from Corynebacterium glutamicum ATCC 13032
27% identity, 93% coverage
CHC_T00009255001 3-phosphoshikimate 1-carboxyvinyltransferase from Chondrus crispus
27% identity, 87% coverage
P23281 3-phosphoshikimate 1-carboxyvinyltransferase 2 (Fragment) from Nicotiana tabacum
29% identity, 73% coverage
- Physiological and differential protein expression analyses of the calcium stress response in the Drynaria roosii rhizome.
Wu, Heliyon 2024 - “...CYP71B10 1.747 0.016 Secondary metabolism ( 7 ) P21357 Phospho-2-dehydro-3-deoxyheptonate aldolase 1 SHKA 42.900 0.000 P23281 3-phosphoshikimate 1-carboxyvinyltransferase 2 EPSPS-2 2.601 0.039 Q9SSE7 Arogenate dehydratase/prephenate dehydratase 2 ADT2 2.848 0.002 W5XMH0 Cinnamate 4-hydroxylase C4H 2.225 0.008 B9VV87 Cinnamate 4-hydroxylase (Fragment) 1.608 0.032 A0A5P8I1Z5 Dihydroflavonol 4-reductase DFR5...”
- “...0.020 F4HVW5 Protein VASCULAR ASSOCIATED DEATH 1 VAD1 1.584 0.001 Secondary metabolism ( 5 ) P23281 3-phosphoshikimate 1-carboxyvinyltransferase 2 (Fragment) EPSPS-2 1.988 0.035 Q5EP59 Phenylalanine ammonia-lyase PAL 3.812 0.028 Q5EP63 Phenylalanine ammonia-lyase PAL 1.894 0.045 Q5EP60 Phenylalanine ammonia-lyase (Fragment) PAL 1.810 0.031 B9VV87 Cinnamate 4-hydroxylase (Fragment)...”
7tbuA / Q5AME2 Crystal structure of the 5-enolpyruvate-shikimate-3-phosphate synthase (epsps) domain of aro1 from candida albicans in complex with shikimate-3-phosphate (see paper)
31% identity, 88% coverage
- Ligand: shikimate-3-phosphate (7tbuA)
SS1G_13550, XP_001585666 hypothetical protein from Sclerotinia sclerotiorum 1980 UF-70
27% identity, 27% coverage
- Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans
Zhao, Frontiers in microbiology 2020 - “...DEGs involved in the biosynthesis of phenylalanine was significantly suppressed ( Figure 4 ). Gene SS1G_13550 is deduced to encode a multifunctional protein, including dehydroquinate synthase (EC 4.2.3.4) (DHQS), 3-dehydroquinate dehydratase (EC 4.2.1.10) (DHQD), shikimate dehydrogenase (E.C. 1.1.1.25) (SKDH), shikimate kinase (EC 2.7.1.71) (SHK) and 5-O-(1-Carboxyvinyl)-3-phosphoshikimate...”
- “...S. sclerotiorum at the early stage of mycoparasitism process by C. minitans , namely SS1G_12793, SS1G_13550, SS1G_03887, SS1G_08569 , and SS1G_00612 . C. minitans probably decreases the resistance of S. sclerotiorum by inhibiting the shikimate pathway and the phenylalanine biosynthesis to profit its parasitization. As a...”
- [Cloning and expression of arom gene of Sclerotinia sclerotiorum].
Yu, Wei sheng wu xue bao = Acta microbiologica Sinica 2006 (PubMed)- GeneRIF: Arom gene encodes a single polypeptide that catalyses steps two to six of the aromatic amino acid (phenylalanine, tyrosine and tryptophan) biosynthetic pathways.
Afu1g13740 pentafunctional polypeptide (AroM), putative from Aspergillus fumigatus Af293
27% identity, 26% coverage
- A Multifaceted Role of Tryptophan Metabolism and Indoleamine 2,3-Dioxygenase Activity in Aspergillus fumigatus-Host Interactions
Choera, Frontiers in immunology 2017 - “...Ortholog in mammals Chorismate biosynthesis AroF Aro3 Afu1g02110 DAHP synthase AroG Aro4 Afu7g04070 AroM Aro1 Afu1g13740 EPSP synthase AroB Aro2 Afu1g06940 Chorismate synthase Aromatic amino acid (AAA) biosynthesis TrpE Trp2 Afu6g12580 Anthranilate synthase TrpC Trp3 Afu1g13090 TrpD Trp4 Afu4g11980 Anthranilate phosphoribosyltransferase TrpC Trp1 Afu1g13090 Phosphoribosylanthranilate isomerase...”
- TrpE feedback mutants reveal roadblocks and conduits toward increasing secondary metabolism in Aspergillus fumigatus
Wang, Fungal genetics and biology : FG & B 2016 - “...and regulation of enzyme in A. fumigatus Enzymes are indicated by their gene designations: AroM (Afu1g13740), shikimate kinase (EC:2.7.1.71), EPSP synthase (EC:2.5.1.19); AroB (Afu1g06940), chorismate synthase (EC:4.2.3.5); TrpC (Afu1g13090), TrpE (Afu6g12580), anthranilate synthase (EC:4.1.3.27); TrpD (Afu4g11980), anthranilate phosphoribosyltransferase (EC:2.4.2.18); TrpC (Afu1g13090), phosphoribosylanthranilate isomerase (EC:5.3.1.24), indole-3-glycerol-phosphate synthase...”
- “...function Alias Protein Ortholog in S.cerevisiae GenBank accession EC number Protein function a Tryptophan biosynthesis Afu1g13740 AroM Aro1 XM_747651 2.7.1.71 Shikimate kinase Afu1g13740 AroM Aro1 2.5.1.19 EPSP b synthase Afu1g06940 AroB Aro2 XM_745350 4.2.3.5 Chorismate synthase Afu6g12580 TrpE Trp2 XM_746043 4.1.3.27 Anthranilate synthase Afu1g13090 TrpC Trp3...”
L7PA93 3-phosphoshikimate 1-carboxyvinyltransferase from Janibacter limosus
30% identity, 94% coverage
6hqvA / G0S061 Pentafunctional arom complex from chaetomium thermophilum (see paper)
28% identity, 27% coverage
- Ligands: nicotinamide-adenine-dinucleotide; zinc ion; glutamic acid; (4s,5r)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid; (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid (6hqvA)
G0S061 Pentafunctional AROM polypeptide from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
28% identity, 27% coverage
AO090012000502 No description from Aspergillus oryzae RIB40
28% identity, 26% coverage
ARO1_EMENI / P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
P07547 shikimate dehydrogenase (NADP+) (EC 1.1.1.25) from Aspergillus nidulans (see paper)
aromA pentafunctional AROM polypeptide; EC 1.1.1.25; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 4.2.3.4 from Emericella nidulans (see 4 papers)
27% identity, 26% coverage
- function: The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis
catalytic activity: 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3- dehydroquinate + phosphate (RHEA:21968)
catalytic activity: 3-dehydroquinate = 3-dehydroshikimate + H2O (RHEA:21096)
catalytic activity: shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+) (RHEA:17737)
catalytic activity: shikimate + ATP = 3-phosphoshikimate + ADP + H(+) (RHEA:13121)
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256)
cofactor: Zn(2+) Note=Binds 2 Zn(2+) ions per subunit
subunit: Homodimer. - CharProtDB Description: Putative pentafunctional AROM polypeptide with 3-dehydroquinate synthase, 3-dehydroquinate dehydratase, shikimate 5-dehydrogenase, shikimate kinase, and EPSP synthase activities; has a predicted role in aromatic amino acid biosynthesis; Source:AspGD
- Biosynthetic gene cluster of cetoniacytone A, an unusual aminocyclitol from the endosymbiotic Bacterium Actinomyces sp. Lu 9419.
Wu, Chembiochem : a European journal of chemical biology 2009 - “...(CGRB) Facilities of Oregon State University. ValA (DQ164098), acbC ( Y18523 ) and aroB ( P07547 ) sequences were retrieved from Genbank at the NCBI database. The complete genome sequence of Frankia alni ACN14a ( NC008278 ) and the sequence of BE-40644 gene cluster ( AB113568...”
Q03321 3-phosphoshikimate 1-carboxyvinyltransferase from Aeromonas salmonicida
26% identity, 96% coverage
- First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
Massot, 3 Biotech 2019 - “...Bordetella pertussis (P12421), Aeromonas salmonicida (Q03321), Rahnella aquatilis (C9EGX2), Escherichia coli (P0A6D3), Salmonella typhimurium (P07637),...”
- A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity
Peng, PloS one 2012 - “...I AroA protein. Class I AroA proteins were obtained from E. coli (P07638), Aeromonas salmonicida (Q03321), Arabidopsis thaliana (P05466), N. tabacum (P23981), Petunia hybrida (P11043), Z. mays (CAA44974), and Bordetella pertussis (P12421). Class II AroA proteins were obtained from Pseudomonas strain PG2982 (P56952), A. tumefaciens strain...”
- Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
Tian, Applied and environmental microbiology 2010 - “...no. P07638), Aeromonas salmonicida (Swiss-Prot Q03321), Arabidopsis thaliana (Swiss-Prot P05466), Nicotiana tabacum (Swiss-Prot P23981), Petunia hybrida...”
- Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
Sun, Applied and environmental microbiology 2005 - “...are from E. coli (P07638), Aeromonas salmonicida (Q03321), Arabidopsis thaliana (P05466), Nicotiana tabacum (P23981), Petunia hybrida (P11043), Zea mays...”
CNAG_03701 3-phosphoshikimate 1-carboxyvinyltransferase from Cryptococcus neoformans var. grubii H99
26% identity, 26% coverage
P0CM23 Pentafunctional AROM polypeptide from Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
P0CM22 Pentafunctional AROM polypeptide from Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)
CNB01990 aromatic amino acid family biosynthesis-related protein from Cryptococcus neoformans var. neoformans JEC21
26% identity, 26% coverage
- Comparative Proteomic Analysis of Rhizoctonia solani Isolates Identifies the Differentially Expressed Proteins with Roles in Virulence.
Prabhukarthikeyan, Journal of fungi (Basel, Switzerland) 2022 - “...exoribonuclease 2(Q4P149), Glyceraldehyde-3-phosphate dehydrogenase 1 (P32635), Inositol-pentakisphosphate 2-kinase (Q4P4C1) and Pentafunctional AROM polypeptide (P0CM22 and P0CM23) were responsible for translation, biosynthesis of sulfur-containing amino acids, protein biosynthesis, glycolysis, phosphorylation and catalytic activities in fungi, respectively [ 81 , 82 , 83 , 84 ]. These proteins...”
- “...methylesterase 1 0.298 Down-regulated Q4P4C1 Inositol-pentakisphosphate 2-kinase 0.307 Down-regulated P0CO60 Putative lipase ATG15 0.333 Down-regulated P0CM23 Pentafunctional AROM polypeptide 0.343 Down-regulated P0CO61 Putative lipase ATG15 0.353 Down-regulated P0CP58 Pescadillo homolog 0.427 Down-regulated Q4P0P0 Eukaryotic translation initiation factor 3 subunit C 0.440 Down-regulated I3ZNU9 Orsellinic acid synthase...”
- Comparative Proteomic Analysis of Rhizoctonia solani Isolates Identifies the Differentially Expressed Proteins with Roles in Virulence.
Prabhukarthikeyan, Journal of fungi (Basel, Switzerland) 2022 - “...(Q4P763), glycylpeptide N-tetradecanoyltransferase (P34809), sulfate adenylyltransferase (Q4P460), glyceraldehyde-3-phosphate dehydrogenase 1 (P32635) and pentafunctional AROM polypeptide (P0CM22), were randomly selected for gene expression analysis. The relative mRNA levels of each transcript were checked in RS15 and RS22. Each genes mRNA levels were normalized to the expression of...”
- “...(Q4P460), 5-3 exoribonuclease 2(Q4P149), Glyceraldehyde-3-phosphate dehydrogenase 1 (P32635), Inositol-pentakisphosphate 2-kinase (Q4P4C1) and Pentafunctional AROM polypeptide (P0CM22 and P0CM23) were responsible for translation, biosynthesis of sulfur-containing amino acids, protein biosynthesis, glycolysis, phosphorylation and catalytic activities in fungi, respectively [ 81 , 82 , 83 , 84 ]....”
- Comprehensive genome-scale metabolic model of the human pathogen Cryptococcus neoformans: A platform for understanding pathogen metabolism and identifying new drug targets
Tezcan, Frontiers in bioinformatics 2023 - “...CNF02630, CNC06150 Riboflavin Metabolism CND01510, CND06120, CNB01460, CNA07220, CNH01620, CNB03030 Histidine Metabolism CNA07990 Phenylalanine Metabolism CNB01990, CNH02650, CNI00560, CNA07880, CNF03410, CNM00820 Phenylalanine, Tyrosine and Tryptophan Biosynthesis CNG04250 Glycerophospholipid Metabolism Further on this issue, the double-gene deletion analysis and comparison with the human genes in the Recon3D...”
PAS_chr3_0506 Pentafunctional arom protein from Komagataella phaffii GS115
28% identity, 27% coverage
A0A2K1XRJ2 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Populus trichocarpa (see paper)
27% identity, 83% coverage
XP_449840 hypothetical protein from Candida glabrata CBS 138
27% identity, 24% coverage
- Molecular analysis and essentiality of Aro1 shikimate biosynthesis multi-enzyme in Candida albicans
Stogios, Life science alliance 2022 - “...ARO1 orthologs Aro1 sequences from C. auris (acc. No. XP_018172187.1 ), C. glabrata (acc. No. XP_449840 ), C. lusitaniae (acc. No. OVF04495.1 ), C. parapsilosis (acc. No. CCE44200 ), C. tropicalis (acc. No. XP_002545280.1 ), C. viswanathii (acc. No. RCK59526.1 ) were obtained from GenBank and...”
- “...Phusion polymerase (NEB). C. albicans DQD1 (acc. no. XP_714872 ), C. glabrata ARO1 (acc. no. XP_449840 ), and C. parapsilosis ARO1 (acc. no. CCE44200 ) were codon-optimized for E. coli expression and synthesized (Codex DNA). All genes were cloned into the pMCSG53 expression vector via Gibson...”
MSMEG_1890, WP_011727983 3-phosphoshikimate 1-carboxyvinyltransferase from Mycolicibacterium smegmatis MC2 155
MSMEG_1890 3-phosphoshikimate 1-carboxyvinyltransferase from Mycobacterium smegmatis str. MC2 155
28% identity, 96% coverage
Pc16g12160 uncharacterized protein from Penicillium rubens
26% identity, 26% coverage
BF0737 3-phosphoshikimate 1-carboxyvinyltransferase from Bacteroides fragilis YCH46
23% identity, 93% coverage
BIF_00329 3-phosphoshikimate 1-carboxyvinyltransferase from Bifidobacterium animalis subsp. lactis BB-12
27% identity, 90% coverage
- Updated Genome Sequence for the Probiotic Bacterium Bifidobacterium animalis subsp. lactis BB-12
Jensen, Microbiology resource announcements 2021 - “...BIF_02255 1720652 14bp29bp [BIF_02256] 1735437 17bp34bp Intergenic(31/+299) BIF_01975 / BIF_00492 1747481 1bp Intergenic(+38/+25) BIF_00693 / BIF_00329 1747488 +A Intergenic(+45/+18) BIF_00693 / BIF_00329 1747489 + CCCCTCACATTT Intergenic(+46/+17) BIF_00693 / BIF_00329 1755127 CG M12I(AT G AT C ) BIF_00612 1755177 +C Coding(2,001/2,019nt) BIF_00102 1755247 2bpGC Coding(1,9301,931/2,019nt) BIF_00102 1756978...”
7m0oA / B5HND8 Dgt-28 epsps (see paper)
27% identity, 92% coverage
- Ligand: phosphate ion (7m0oA)
TERG_03083 3-phosphoshikimate 1-carboxyvinyltransferase from Trichophyton rubrum CBS 118892
26% identity, 32% coverage
NMCC_1342 5-enolpyruvoylshikimate-3-phosphate synthase from Neisseria meningitidis 053442
27% identity, 81% coverage
HMPREF1058_RS13970 3-phosphoshikimate 1-carboxyvinyltransferase from Phocaeicola vulgatus CL09T03C04
22% identity, 92% coverage
XNR_1588 3-phosphoshikimate 1-carboxyvinyltransferase from Streptomyces albidoflavus
28% identity, 91% coverage
A3TGI7 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Janibacter sp. (see paper)
27% identity, 93% coverage
BT2186 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Bacteroides thetaiotaomicron VPI-5482
23% identity, 93% coverage
- mutant phenotype: # This protein is essential, but a close homolog (HMPREF1058_RS13970) is cofit with other chorismate synthesis genes. Furthermore, this gene (also known as BT_RS11065) complemented an aroA- mutant of E. coli (data of PMID:39375541)
AAFF35_21465 3-phosphoshikimate 1-carboxyvinyltransferase from Pedobacter sp. FW305-3-2-15-E-R2A2
25% identity, 93% coverage
B118_RS0101230 3-phosphoshikimate 1-carboxyvinyltransferase from Salinispora pacifica CNR114
28% identity, 91% coverage
Echvi_0122 3-phosphoshikimate 1-carboxyvinyltransferase from Echinicola vietnamensis DSM 17526
23% identity, 91% coverage
- GapMind: Automated Annotation of Amino Acid Biosynthesis
Price, mSystems 2020 - “...standard threonine synthase or ThrC (PGA1_c06310) Diverged Chorismate: AroA Echinicola vietnamensis KMM 6221, DSM 17526 Echvi_0122 may be a diverged AroA; it appears to be essential Diverged Cysteine: CysE Echinicola vietnamensis KMM 6221, DSM 17526 Echvi_0221 may be a diverged serine acetyltransferase; it appears to be...”
PG1944 3-phosphoshikimate 1-carboxyvinyltransferase from Porphyromonas gingivalis W83
24% identity, 93% coverage
P19670 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 from Bacillus subtilis (strain 168)
25% identity, 94% coverage
Mb3256 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE AROA (5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE) (EPSP SYNTHASE) (EPSPS) from Mycobacterium bovis AF2122/97
27% identity, 95% coverage
2o0bA / P9WPY5 Mycobacterium tuberculosis epsp synthase in complex with s3p (partially photolyzed)
27% identity, 95% coverage
- Ligand: shikimate-3-phosphate (2o0bA)
AROA_MYCTU / P9WPY5 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
P9WPY5 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) from Mycobacterium tuberculosis (see paper)
Rv3227 3-phosphoshikimate 1-carboxyvinyltransferase from Mycobacterium tuberculosis H37Rv
NP_217744 3-phosphoshikimate 1-carboxyvinyltransferase from Mycobacterium tuberculosis H37Rv
27% identity, 95% coverage
- function: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
catalytic activity: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate (RHEA:21256)
subunit: Monomer. - From Genome to Drugs: New Approaches in Antimicrobial Discovery
Serral, Frontiers in pharmacology 2021 - “...key biochemical intermediate, being a precursor for aromatic amino acids. Within this pathway, 3-phosphoshikimate 1-carboxyvinyltransferase (Rv3227) could be selected for further studies. We found that it is druggable, essential, and not present in humans, and appears overexpressed under different conditions that mimic infections. TABLE 2 Mtb...”
- “...Gut microbiome Essentiality Inositol-3-phosphate synthase (Ino1, Rv0046c) 0,946 Yes Low Low Low Yes 3-Phosphoshikimate 1-carboxyvinyltransferase (Rv3227) 0,696 Yes High Low Low Yes O-Acetylhomoserine aminocarboxypropyltransferase (Rv3340) 0,679 Yes Low Low High Yes 3-Oxoacyl-[acyl-carrier-protein] synthase 2 (Rv2246) 0,709 Yes Low Low Low Yes Octanoyltransferase (Rv2217 0,703 Yes Low...”
- Identification of Anti-tuberculosis Compounds From Aurone Analogs
Yang, Frontiers in microbiology 2020 - “...a HisTrap column via elution with a series of concentrations of imidazole. PCR amplified EPSP (Rv3227) and Cs (Rv2540c) coding sequences were cloned into pET28a(+). PCR was conducted using the primers Rv2540c-F tata catatg gtgttgcgctggatcacc and Rv2540c-R: tata ggatcc ttaaccggagacccgc to amplify Rv2540c from genomic DNA...”
- “...3 min at 72C, and then 10 min at 72C for final extension. To amplify Rv3227 from genomic DNA of Mtb CDC1551, PCR was conducted using the primers Rv3227-F: tata catatg gtgaagacatggccagcc and Rv3227-R: tata ggatcc actcgtcgtagtcgccgg with the following program: denaturation for 5 min at...”
- Integrative proteomic and glycoproteomic profiling of Mycobacterium tuberculosis culture filtrate
Tucci, PloS one 2020 - “...This work GlnA1 Rv2220 T36 NO This work AceE Rv2241 S32 NO This work AroA Rv3227 S349 NO This work SahH Rv3248c T473 NO This work Rv3273 Rv3273 S735 NO 10 This work Conserved hypotheticals Rv0311 Rv0311 S10 NO This work Rv0566c Rv0566c T52, S53 &...”
- Identification of attractive drug targets in neglected-disease pathogens using an in silico approach
Crowther, PLoS neglected tropical diseases 2010 - “...inha (nadh-dependent enoyl-acp reductase) 511 2 Rv2220 glutamine synthetase glna1 (glutamine synthase) (gs-i) 451 3 Rv3227 3-phosphoshikimate 1-carboxyvinyltransferase aroa (5-enolpyruvylshikimate-3-phosphate synthase) (epsp synthase) (epsps) 426 4 Rv3581c probable 2c-methyl-d-erythritol 2,4-cyclodiphosphate synthase ispf (mecps) 406 4 Rv2763c dihydrofolate reductase dfra (dhfr) (tetrahydrofolate dehydrogenase) 406 4 Rv2537c 3-dehydroquinate...”
- Genomic-scale prioritization of drug targets: the TDR Targets database
Agüero, Nature reviews. Drug discovery 2008 - “...150 Rv3803c Secr. Mpt51/Mpb51 antigen 85 complex C (FbpD) 160 Rv0983 Serine protease PepD 150 Rv3227 3-P-shikimate 1-Carboxyvinyltransferase (AroA) 160 Rv3427c Possible transposase 150...”
- targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis
Raman, BMC systems biology 2008 - “...best used at the post-identification analysis stage. For example, proteins such as TrpD (Rv2192c), AroA (Rv3227), RibC (Rv1412) do not appear to be expressed in any of the experiments considered. Comparison with Anti-targets An ideal target should not only have specific recognition to the drug directed...”
- Investigating the metabolic capabilities of Mycobacterium tuberculosis H37Rv using the in silico strain iNJ661 and proposing alternative drug targets
Jamshidi, BMC systems biology 2007 - “...78 2 LeuD Rv2987c 14 2 TrpD Rv2192c 10 4 DapB Rv2773c 2 3 AroA Rv3227 20 3 AroC Rv2540c 20 3 AroE Rv2537c 11 4 AroG Rv2178c 11 4 AroQ Rv2537c 11 4 ilvG (acetolactate synthase) Rv1820 3 3 ilvX (acetolactate synthase) Rv3509c 3 3...”
- Novel genetic polymorphisms that further delineate the phylogeny of the Mycobacterium tuberculosis complex
Huard, Journal of bacteriology 2006 - “...and aroA117 are located intermediate of Rv3226c and aroA (Rv3227); aroA285 is located within aroA. g Located intermediate of M. bovis mb1581 (Rv1556) and mmpL6...”
- EPSP synthase flexibility is determinant to its function: computational molecular dynamics and metadynamics studies.
Timmers, Journal of molecular modeling 2017 (PubMed)- GeneRIF: the new findings presented here indicate how the hydrophobic regions modulate the flexibility of MtEPSP synthase, and they highlight the importance of considering these dynamic features in drug design projects employing this enzyme as a target. Graphical abstract The flexibility of EPSP synthase as a function of the pincer angles.
3swgA / O67315 Aquifex aeolicus mura in complex with udp-n-acetylmuramic acid and covalent adduct of pep with cys124 (see paper)
26% identity, 89% coverage
- Ligand: (2r)-2-{[(2r,3r,4r,5s,6r)-3-(acetylamino)-2-{[(s)-{[(r)-{[(2r,3s,4r,5r)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2h)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2h-pyran-4-yl]oxy}propanoic acid (3swgA)
An08g06810 uncharacterized protein from Aspergillus niger
27% identity, 26% coverage
NBX27_08175 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Erysipelothrix rhusiopathiae
25% identity, 94% coverage
- Human Erysipelothrix rhusiopathiae infection via bath water - case report and genome announcement
Zautner, Frontiers in cellular and infection microbiology 2022 - “...MIC of 32.0 mg/L. Table5 Enzymes involved in peptidoglycan biosynthesis. Locus tag Gene Predicted function NBX27_08175 murA UDP-N-acetylglucosamine-1-carboxyvinyltransferase NBX27_02770 murB UDP-N-acetylmuramate dehydrogenase NBX27_02935 murC UDP-N-acetylmuramateL-alanine ligase or UDP-N-acetylmuramateL-serine ligase? NBX27_02805 murD UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase NBX27_04865 murE UDP-N-acetylmuramoylalanyl-D-glutamateL-alanine ligase NBX27_03210 murF UDP-N-acetylmuramoyl-tripeptideD-alanyl-D-alanine ligase NBX27_02800 mraY Phospho-N-acetylmuramoyl-pentapeptide-transferase NBX27_04480 murG...”
XP_002773648 3-dehydroquinate synthase, putative from Perkinsus marinus ATCC 50983
37% identity, 5% coverage
- The search for the missing link: a relic plastid in Perkinsus?
Fernández, International journal for parasitology 2011 - “...have identified several genes of the shikimate pathway, including chorismate synthase (XP_002773541) and a gene (XP_002773648) with a match to the pentafunctional arom protein, which catalyses five consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis, 3.4.6. ER-associated protein degradation (ERAD) system The ERAD pathway is...”
NMB0011 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Neisseria meningitidis MC58
26% identity, 90% coverage
Q5F5K6 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
26% identity, 90% coverage
lmo2552 highly similar to UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Listeria monocytogenes EGD-e
Q8Y4A2 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
LMRG_01695 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Listeria monocytogenes 10403S
24% identity, 94% coverage
- Listeria monocytogenes exposed to antimicrobial peptides displays differential regulation of lipids and proteins associated to stress response
Stincone, Cellular and molecular life sciences : CMLS 2022 - “...Fengycin Fengycin/nisin Fengycin/nisin gshAB lmo0927 dacA/lmo2120 lmo2555 lmo2552 4.65 Up Fengycin/nisin lmo1539 a 13 mutant, counteracting the complete loss of...”
- “...of both UDPN-acetylglucosamine 1-carboxyvinyltransferase (MurZ, lmo2552 gene) and putative glycerol uptake facilitator protein (GlpF-2, lmo1539 gene),...”
- Sugar Modification of Wall Teichoic Acids Determines Serotype-Dependent Strong Biofilm Production in Listeria monocytogenes
Park, Microbiology spectrum 2022 - “...acid glycosylation protein lmo2549 yfdG , partially similar 10 murZ UDP- N -acetylglucosamine 1-carboxyvinyltransferase 2 lmo2552 murAB , partially similar 11 ami Autolysin lmo2558 Absent 12 rmlT Putative glycosyltransferase lmo1080 Absent 13 rmlA Glucose-1-phosphate thymidylyltransferase 1 lmo1081 Absent 14 rmlC dTDP-4-dehydrorhamnose 3,5-epimerase lmo1082 Absent 15 rmlB...”
- Proteomic dataset of Listeria monocytogenes exposed to sublethal concentrations of free and nanoencapsulated nisin
Pinilla, Data in brief 2022 - “...of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template Q8Y4A2 lmo2552 murZ Nis / LNis UDP- N -acetylglucosamine 1-carboxyvinyltransferase; cell wall formation Q8Y8D4 lmo0974 dltA Nis / LNis D-alanine-poly(phosphoribitol) ligase subunit 1; catalyzes the first step in the D-alanylation of lipoteichoic...”
- Imbalance of peptidoglycan biosynthesis alters the cell surface charge of Listeria monocytogenes
Schulz, Cell surface (Amsterdam, Netherlands) 2022 - “...addition, we identified mutations that mapped to genes associated with peptidoglycan biosynthesis ( murZ ( lmo2552 ), reoM ( lmo1503), prpC ( lmo1821 ), pbpA1 ( lmo1892 )), peptidoglycan hydrolysis ( cwlO ( lmo2505, spl ), ftsX ( lmo2506 ), ftsE ( lmo2507 )) and wall...”
- Listeria monocytogenes utilizes the ClpP1/2 proteolytic machinery for fine-tuned substrate degradation at elevated temperatures
Balogh, RSC chemical biology 2022 - “...family transcriptional regulator a lmo2489 ( uvrB ) Q8Y4F5 UvrABC system protein B, excision nuclease lmo2552 ( murZ ) Q8Y4A2 UDP- N -acetylglucosamine 1-carboxyvinyltransferase 2 lmo2712 Q8Y3W7 Putative gluconate kinase (Pentose phosphate pathway) a a The functions of not annotated proteins were derived from BLAST searches....”
- Colonisation dynamics of Listeria monocytogenes strains isolated from food production environments
Gray, Scientific reports 2021 - “...system mannose-specific IIC component Lmo0783 fig|1639.4014.peg.1297 4.18 manX G PTS system mannose-specific IIAB component 2.7.1.191 lmo2552 fig|1639.4014.peg.192 3.7 murZ M UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2.5.1.7 Ribosome lmo1542 fig|1639.4024.peg.1293 3.82 rplU J LSU ribosomal protein L21p 7545 48h lmo0250 fig|1639.4024.peg.1620 3.36 rplJ J LSU ribosomal protein L10p (P0) lmo2629...”
- PrkA controls peptidoglycan biosynthesis through the essential phosphorylation of ReoM
Wamp, eLife 2020 - “...pSH246 bla erm bgaB gpsB (lmo1888) Rismondo et al., 2016 pJR68 bla erm bgaB murZ (lmo2552) Rismondo et al., 2017 pJR71 P help -lacO-murZ lacI neo Rismondo et al., 2017 pJR65 P help -lacO-reoM lacI neo this work pJR70 P help -lacO-reoY lacI neo this work...”
- “...1/2a strain Glaser et al., 2001 LMJR19 gpsB (lmo1888) Rismondo et al., 2016 LMJR104 murZ (lmo2552) Rismondo et al., 2017 LMJR116 attB:: P help -lacO-murA lacI neo Rismondo et al., 2017 LMJR123 murA (lmo2526) attB:: P help -lacO-murA lacI neo Rismondo et al., 2017 LMJR138 clpC...”
- Suppressor Mutations Linking gpsB with the First Committed Step of Peptidoglycan Biosynthesis in Listeria monocytogenes
Rismondo, Journal of bacteriology 2017 - “...to MurA (encoded by the lmo2526 gene), MurZ (lmo2552) is one of the two UDP-N-acetylglucosamine 1-carboxyvinyltransferases that mediate the transfer of an...”
- “...additional nucleotides inserted into the murZ gene (lmo2552; 153-fold coverage, 98.7% allele frequency), which encodes one of the two UDP-N-acetylglucosamine...”
- More
- Proteomic dataset of Listeria monocytogenes exposed to sublethal concentrations of free and nanoencapsulated nisin
Pinilla, Data in brief 2022 - “...activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template Q8Y4A2 lmo2552 murZ Nis / LNis UDP- N -acetylglucosamine 1-carboxyvinyltransferase; cell wall formation Q8Y8D4 lmo0974 dltA Nis / LNis D-alanine-poly(phosphoribitol) ligase subunit 1; catalyzes the first step in the D-alanylation of...”
- Listeria monocytogenes utilizes the ClpP1/2 proteolytic machinery for fine-tuned substrate degradation at elevated temperatures
Balogh, RSC chemical biology 2022 - “...lmo2489 ( uvrB ) Q8Y4F5 UvrABC system protein B, excision nuclease lmo2552 ( murZ ) Q8Y4A2 UDP- N -acetylglucosamine 1-carboxyvinyltransferase 2 lmo2712 Q8Y3W7 Putative gluconate kinase (Pentose phosphate pathway) a a The functions of not annotated proteins were derived from BLAST searches. Interestingly, the number of...”
- PASTA kinase-dependent control of peptidoglycan synthesis via ReoM is required for cell wall stress responses, cytosolic survival, and virulence in Listeria monocytogenes
Kelliher, PLoS pathogens 2021 - “...cond parent strain. Isolate Locus Name Mutation Variant Function/Description PCS1 Lmrg_01600 G2282263A E337K Hypothetical protein Lmrg_01695 murZ C2583617A Intergenic (39 bp upstream of start) MurA paralog PCS2 Lmrg_00741 oatA C1277896T Silent Peptidoglycan o -acetyltransferase Lmrg_01780 clpP G2496832A R112H Catalytic subunit of Clp protease Lmrg_01945 G2789642A A241T...”
- “...A241T ATP-binding subunit of multidrug efflux pump PCS6 Lmrg_00741 oatA C1277896T Silent Peptidoglycan o -acetyltransferase Lmrg_01695 murZ C2582699CT Frameshift, premature stop codon MurA paralog Lmrg_01945 G2789642A A241T ATP-binding subunit of multidrug efflux pump Lmrg_01919 C2822334A Silent Hypothetical protein PCS7 Lmrg_01780 clpP G2496732A D79N Catalytic subunit of...”
Q5GRP6 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Wolbachia sp. subsp. Brugia malayi (strain TRS)
25% identity, 68% coverage
Q9ZLI6 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Helicobacter pylori (strain J99 / ATCC 700824)
24% identity, 90% coverage
B5F9P4 UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) from Aliivibrio fischeri (see paper)
B6DVJ7 UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) from Aliivibrio fischeri (see paper)
26% identity, 89% coverage
3vcyA / B5F9P4 Structure of mura (udp-n-acetylglucosamine enolpyruvyl transferase), from vibrio fischeri in complex with substrate udp-n- acetylglucosamine and the drug fosfomycin. (see paper)
26% identity, 89% coverage
- Ligands: [(1r)-1-hydroxypropyl]phosphonic acid; uridine-diphosphate-n-acetylglucosamine (3vcyA)
MURA1_STRP2 / A0A0H2ZNL3 UDP-N-acetylglucosamine 1-carboxyvinyltransferase; Enoylpyruvate transferase; EPT; UDP-N-acetylglucosamine enolpyruvyl transferase; EC 2.5.1.7 from Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (see paper)
SPD_0967 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Streptococcus pneumoniae D39
24% identity, 94% coverage
- function: Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine (By similarity). Target for the antibiotic fosfomycin. Involved in heteroresistance to antibiotic fosfomycin. Heteroresistance is the ability of a clonal population to grow one or several subpopulations at a frequency of 10(-7) to 10(-3) in the presence of a higher antibiotic concentration than that predicted to be effective by measurement of the minimum inhibitory concentration (MIC) (PubMed:23571543).
catalytic activity: phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = UDP- N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + phosphate (RHEA:18681)
disruption phenotype: No heteroresistance to fosfomycin. - Negative regulation of MurZ and MurA underlies the essentiality of GpsB- and StkP-mediated protein phosphorylation in Streptococcus pneumoniae D39
Tsui, Molecular microbiology 2023 - “...Kristich, 2012 ). The two homologs in S. pneumoniae strains were annotated as MurZ (MurA2) (Spd_0967) and MurA (MurA1) ( Spn )(Spd_1764) ( Hoskins et al., 2001 ) ( Fig. 1 ). The MurA-family homolog, which is the sole enzyme present in Gram-negative bacteria ( Brown...”
- “...duplicated regions of sup gpsB -23 and sup gpsB - 8 - 10 contain murZ (spd_0967 ) ( Fig. 2A , 3 , and S1B S1C ). Since GpsB plays a role in activation of pneumococcal StkP Ser/Thr kinase activity ( Fleurie et al., 2014 ,...”
- Movement dynamics of divisome proteins and PBP2x:FtsW in cells of Streptococcus pneumoniae
Perez, Proceedings of the National Academy of Sciences of the United States of America 2019 - “...first committed step of PG synthesis ( 47 , 48 ). Deletion of murA1 ( spd_0967 ; also called murZ ) does not significantly alter growth, cell morphology, or FtsZ treadmilling velocity in C+Y, pH 7.1 ( SI Appendix , Fig. S26 C ). However, the...”
SMU_RS07130 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Streptococcus mutans UA159
23% identity, 94% coverage
- Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome of Streptococcus mutans
Qin, Microbiology spectrum 2024 - “...3.2 Glucose-6-phosphate isomerase, GPI SMU_RS02625 2.6 4 UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase, MurD SMU_RS08200 2.4 Isoprenyl transferase, UppS SMU_RS07130 2.3 UDP-N-acetylglucosamine 1-carboxyvinyltransferase, MurA2 SMU_RS04475 2 2 UDP-N-acetylmuramate dehydrogenase, MurB SMU_RS07620 2 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase, MurE Cell division SMU_RS02635 7 FtsQ-type POTRA domain-containing protein, FtsQ SMU_RS02645 6.5 Cell division protein, FtsZ...”
YP_003431998 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Hydrogenobacter thermophilus TK-6
24% identity, 94% coverage
- Phylogenetic position of aquificales based on the whole genome sequences of six aquificales species
Oshima, International journal of evolutionary biology 2012 - “...YP_003432936 ATP-dependent protease YP_003433556 UDP-N-acetylglucosamine pyrophosphorylase YP_003431738 putative metalloprotease YP_003431749 diaminopimelate decarboxylase YP_003431809 dihydrodipicolinate reductase YP_003431998 UDP-N-acetylglucosamine 1-carboxyvinyltransferase YP_003432481 ribosomal protein S20 YP_003432953 queuine tRNA-ribosyltransferase YP_003431834 ATP-dependent protease La YP_003431839 tRNA delta(2)-isopentenylpyrophosphate transferase YP_003431873 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase YP_003431915 ribonuclease III YP_003432036 riboflavin synthase alpha chain YP_003432044...”
B9H01_RS05665, ZY05719_05605 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Streptococcus suis
25% identity, 94% coverage
SSA_0791 UDP-N-acetylglucosamine-like protein, putative from Streptococcus sanguinis SK36
25% identity, 94% coverage
- Genome-wide essential gene identification in Streptococcus sanguinis
Xu, Scientific reports 2011 - “...knockouts in S. sanguinis . Double mutant Donor Product Name Acceptor CFU Gel Essential Ssx_0791::1494 SSA_0791 UDP-N-acetylglucosamine 1-carboxyvinyltransferase Ssx_1494 0 yes Ssx_0578::2195 SSA_0578 nicotinic acid mononucleotide adenylyltransferase Ssx_2195 0 yes Ssx_0352::1188 SSA_0352 ribonuclease HIII Ssx_1188 0 yes Ssx_2168::1827 SSA_2168 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase Ssx_1827 >5000 1-band No...”
MURA1_STRPI / B1IBM3 UDP-N-acetylglucosamine 1-carboxyvinyltransferase; Enoylpyruvate transferase; EPT; UDP-N-acetylglucosamine enolpyruvyl transferase; EC 2.5.1.7 from Streptococcus pneumoniae (strain Hungary19A-6) (see paper)
24% identity, 94% coverage
- function: Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine (By similarity). Target for the antibiotic fosfomycin.
catalytic activity: phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = UDP- N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + phosphate (RHEA:18681)
CV_0440 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Chromobacterium violaceum ATCC 12472
24% identity, 90% coverage
Q5M376 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311)
24% identity, 94% coverage
6q03A / Q18CL1 Crystal structure of mura from clostridium difficile in the presence of udp-n-acetyl-alpha-d-muramic acid with modified cys116 (s-[(1s)-1- carboxy-1-(phosphonooxy)ethyl]-l-cysteine)
24% identity, 94% coverage
- Ligand: (2r)-2-{[(2r,3r,4r,5s,6r)-3-(acetylamino)-2-{[(s)-{[(r)-{[(2r,3s,4r,5r)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2h)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2h-pyran-4-yl]oxy}propanoic acid (6q03A)
CDR20291_0122 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 from Clostridium difficile R20291
CD0123 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Clostridium difficile 630
24% identity, 94% coverage
- Proteomic Signatures of Clostridium difficile Stressed with Metronidazole, Vancomycin, or Fidaxomicin
Maaß, Cells 2018 - “...protein (HSP-70 cofactor) x CDIF630erm_04005 MnmG CDR20291_3535 tRNA uridine 5-carboxymethyl-aminomethyl modification enzyme x CDIF630erm_00239 MurA CDR20291_0122 UDP- N -acetylglucosamine 1-carboxyvinyltransferase x CDIF630erm_03828 Prs CDR20291_3351 ribose-phosphate pyrophosphokinase x CDIF630erm_00260 SecA1 CDR20291_0142 protein translocase subunit SecA1 x CDIF630erm_01292 MreB2 CDR20291_0982 rod shape-determining protein MreB x x CDIF630erm_03832 MurC...”
- Investigation of the Cross-talk Mechanism in Caco-2 Cells during Clostridium difficile Infection through Genetic-and-Epigenetic Interspecies Networks: Big Data Mining and Genome-Wide Identification
Li, Frontiers in immunology 2017 - “...genes ( 19 ). Seven C. difficile proteins (CD2664, CD2335, CD3550, CD0198, CD1225, CD0130, and CD0123) in the HPN of the early stage of CDI (Figure S3 in Supplementary Material) and three C. difficile proteins (CD2588, CD1816, and CD0130) in the HPN of the late stage...”
- “...difficile proteins (CD2664, CD2335, CD0067, CD3550, CD3540, CD0198, CD1255, CD2714, CD3256, CD1316, CD0095, CD2739, and CD0123) in the HPN of the early stage of CDI (Figure S3 in Supplementary Material) and 15 C. difficile proteins (CD3170, CD2588, CD2744, CD2771, CD2462, CD3304, CD2781, CD3540, CD1145, CD2461, CD2793,...”
PSPPH_4139 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Pseudomonas syringae pv. phaseolicola 1448A
26% identity, 94% coverage
- Transcriptional profile of P. syringae pv. phaseolicola NPS3121 at low temperature: physiology of phytopathogenic bacteria
Arvizu-Gómez, BMC microbiology 2013 - “...protein 1.72 PSPPH_3288 predicted periplasmic lipoprotein 2.05 PSPPH_3810 lipoprotein 1.71 PSPPH_3916 membrane protein, putative 1.86 PSPPH_4139 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.55 PSPPH_4669 acetyltransferase, GNAT family 1.73 PSPPH_4682 lipopolysaccharide biosynthesis protein, putative 3.03 PSPPH_5220 inner membrane protein, 60 kDa 1.61 Cluster 5: Genes involved in motility PSPPH_0730 type IV...”
- “...cell wall biogenesis and membrane synthesis were identified (Cluster 4). These include the murA gene (PSPPH_4139) that is involved in peptidoglycan synthesis (a major component of cell wall), the PSPPH_4682 gene involved in polysaccharide synthesis, as well as three genes PSPPH_4669, PSPPH_3226, and galU (PSPPH_2260) that...”
ACIAD0660 UDP-N-acetylglucosamine 1-carboxyvinyltransferase (Enoylpyruvate transferase) (UDP-N-acetylglucosamine enolpyruvyl transferase) (EPT) from Acinetobacter sp. ADP1
23% identity, 94% coverage
- iTRAQ-Based Comparative Proteomic Analysis of Acinetobacter baylyi ADP1 Under DNA Damage in Relation to Different Carbon Sources
Jiang, Frontiers in microbiology 2019 - “...Glutamate synthase subunit beta argJ ACIAD0650 5.75 8.47 10.67 8.87 UDP- N -acetylglucosamine 1-carboxyvinyltransferase murA ACIAD0660 1.33 1.25 1.32 1.29 O -methyltransferase protein bioC ACIAD0858 2.4 1.96 1.77 1.32 Acyl carrier protein acpP ACIAD0872 3.87 3.87 4.21 5.11 Glycerol kinase glpK ACIAD0930 2.33 3.63 3.53 1.39...”
- “...cis - trans isomerase fklB ACIAD0065 1.33 2.05 1.85 1.56 UDP- N -acetylglucosamine 1-carboxyvinyltransferase murA ACIAD0660 1.33 1.25 1.32 1.29 Peptidylprolyl isomerase ppiD ACIAD1409 2.33 2.38 1.5 1.69 Peptidyl-prolyl cis-trans isomerase precursor (PPIase) surA ACIAD2372 1.74 1.29 1.98 1.21 Lyase Threonine synthase thrC ACIAD0263 2.47 1.71...”
P56189 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Helicobacter pylori (strain ATCC 700392 / 26695)
HP0648 UDP-N-acetylglucosamine enolpyruvyl transferase (murZ) from Helicobacter pylori 26695
23% identity, 90% coverage
aroA / AAA21937.1 3-phosphoshikimate-1-carboxyvinyltransferase, partial from Aeromonas salmonicida (see paper)
24% identity, 65% coverage
BL107_11081 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Synechococcus sp. BL107
27% identity, 64% coverage
- A Sample-to-Sequence Protocol for Genus Targeted Transcriptomic Profiling: Application to Marine Synechococcus
Pitt, Frontiers in microbiology 2016 - “...2.06 0.19 BL107_06684 116,065,438 Sucrose-phosphate synthase 1.39 0.27 BL107_12250 116,065,819 ACSS; acetyl-CoA synthetase 1.10 0.30 BL107_11081 116,064,848 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.19 0.22 BL107_14110 116,066,191 crtW; beta-carotene ketolase (CrtW type) 1.85 0.37 21: INORGANIC ION TRANSPORT AND METABOLISM 21.2 NITROGEN METABOLISM BL107_06834 116,065,468 nirA; ferredoxin-nitrite reductase 3.94 0.18...”
A1S_0685 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Acinetobacter baumannii ATCC 17978
E5A72_RS10790 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Acinetobacter baumannii ATCC 17978
24% identity, 94% coverage
5u4hA / A0A0R0VXX6 1.05 angstrom resolution crystal structure of udp-n-acetylglucosamine 1-carboxyvinyltransferase from acinetobacter baumannii in covalently bound complex with (2r)-2-(phosphonooxy)propanoic acid.
24% identity, 94% coverage
- Ligand: (2r)-2-(phosphonooxy)propanoic acid (5u4hA)
HSISS4_01465 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Streptococcus salivarius
23% identity, 94% coverage
ZMO1724 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Zymomonas mobilis subsp. mobilis ZM4
26% identity, 70% coverage
P0DC46 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 from Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315)
23% identity, 93% coverage
- Pangenome and genomic taxonomy analyses of Leuconostoc gelidum and Leuconostoc gasicomitatum
Johansson, BMC genomics 2022 - “...Dellaglioa algida A0A0R1HHE0, Carnobacterium maltaromaticum K8E679, Enterococcus faecium A0A133CSM8, Lactococcus lactis Q9CIP4 and Streptococcus pyogenes P0DC46. Supplementary Fig. 2. Multiple alignment ofrepresentative FusA protein sequences from different genera of LAB and from Staphylococcus aureus : The FusAsequences used in the alignment are: Leuconostocgasicomitatum A0A175CRG2, Fructobacillus sp....”
NP_966909 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Wolbachia endosymbiont of Drosophila melanogaster
24% identity, 68% coverage
- Cloning, expression and characterization of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Wolbachia endosymbiont of human lymphatic filarial parasite Brugia malayi
Shahab, PloS one 2014 - “...(WP_009875898.1), Acinetobacter calcoaceticus (WP_005049013.1), Wolbachia endosymbiont of Onchocerca ochengi (YP_006555880.1), Wolbachia endosymbiont of Drosophila melanogaster (NP_966909.1), Wolbachia endosymbiont strain TRS of Brugia malayi (YP_198570.1), Wolbachia endosymbiont of Culex quinquefasciatus (WP_007302175.1), Rickettsia prowazekii (NP_220950.1) and Aquifex aeolicus (NP_213879.1). Viewing and re-annotation were done on FigTree v1.4 (...”
- “...2 ). It exhibits 88.94 and 80.71% homology with MurA of Wolbachia of Drosophila melanogaster (NP_966909) and Culex molestus (CDH88571) respectively. Varying degree of similarity was observed with others bacterial species such as, 41.05% with Escherichia coli (WP_023568349), 42.24% with Vibrio cholerae (WP_001887759), 40.57% with Haemophilius...”
Q99Z78 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 from Streptococcus pyogenes serotype M1
23% identity, 93% coverage
BSU36760 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Bacillus subtilis subsp. subtilis str. 168
P70965 UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 from Bacillus subtilis (strain 168)
25% identity, 94% coverage
KP1_4910 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Klebsiella pneumoniae NTUH-K2044
26% identity, 96% coverage
Q8RIQ1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) from Fusobacterium nucleatum subsp. nucleatum (see paper)
FN1520 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
20% identity, 94% coverage
- Proteomics of Fusobacterium nucleatum within a model developing oral microbial community
Hendrickson, MicrobiologyOpen 2014 - “...FN0060, FN0406, FN0525, FN0580, FN1155, FN1161, FN1211, FN1225, FN1326, FN1454, FN1455, FN1456, FN1457, FN1458, FN1461, FN1520, FN1991. Table 7 Fatty acid biosynthesis. FnPg versus Fn FnSg versus Fn FnPgSg versus Fn FnPgSg versus FnPg PgSg versus FnPg FnPgSg versus FnSg Total 11 11 10 10 11...”
- “...FN0177, FN0265, FN0562, FN1010, FN1155, FN1211, FN1225, FN1326, FN1451, FN1452, FN1455, FN1456, FN1457, FN1458, FN1461, FN1520, FN1978, FN2013, FN2017. Stress Studies with the other two organisms, Pg and Sg, found evidence of physiological support between the species (Kuboniwa etal. 2009 ; Hendrickson etal. 2012 ). DNA...”
- Probing antibacterial drugs for Fusobacterium nucleatum subsp. nucleatum ATCC 25586 targeting UDP-N-acetylglucosamine 1-carboxyltransferase
Saputri, Journal of advanced pharmaceutical technology & research 2023 - “...and constructed using AlphaFold 2 algorithm based on the protein sequence from the UniProt database (Q8RIQ1). Rigid molecular docking was employed to form ligandprotein complex, where the docking score or binding affinity was taken as the parameter for potential interaction. In general, the molecular docking methodology...”
- “...acid sequence of UDP-N-acetylglucosamine 1-carboxyltransferase was obtained from the UniProt database using the access codes Q8RIQ1. The three-dimensional (3D) structure of the protein was probed using the AlphaFold 2 algorithm. The protein sequence was entered in the sequence entry part of Google Colab Pro to carry...”
- Target identification in Fusobacterium nucleatum by subtractive genomics approach and enrichment analysis of host-pathogen protein-protein interactions
Kumar, BMC microbiology 2016 - “...with accession number S. No. Uniprot ID No. of Proteins KEGG pathway KEGG ID 1 Q8RIQ1, Q8RDQ3, Q8RDQ2, Q8RDQ1, Q8R635, Q8R5N5, Q8RDP8, Q8RDQ4, Q8RG00, Q8RFV2, Q8REF2, Q8REA2 12 Peptidoglycan biosynthesis fnu00550 2 Q8RFU2, Q8R691, Q8R6A2, Q8RE91, Q8RFA8 5 Lipopolysaccharide biosynthesis fnu00540 3 Q8RFB7, Q8RED6, Q8R612, Q8R609,...”
Q712I1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Streptomyces tendae
26% identity, 56% coverage
WP_038089003 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Acidihalobacter prosperus
26% identity, 71% coverage
- Multiple Osmotic Stress Responses in Acidihalobacter prosperus Result in Tolerance to Chloride Ions
Dopson, Frontiers in microbiology 2016 - “...Osmoregulation, Cell Envelope, and Its Integrity WP_038086993 Osmolarity response regulator, OmpR Unique d NA e WP_038089003 UDP-N-acetylglucosamine 1-carboxyvinyltransferase, murA Unique NA WP_038093711 ADP-L-glycero-D-mannoheptose-6-epimerase, RfaD Unique NA WP_038088939 D-alanineD-alanine ligase, DdL Unique NA WP_038089461 Ectoine ABC transporter solute-binding protein 55.3 1.6 WP_052064239 MlaC, ABC transporter 10.5 1.8...”
MURA_ENTCC / P33038 UDP-N-acetylglucosamine 1-carboxyvinyltransferase; Enoylpyruvate transferase; UDP-N-acetylglucosamine enolpyruvyl transferase; EPT; EC 2.5.1.7 from Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCTC 10005 / WDCM 00083 / NCDC 279-56) (see 2 papers)
WP_013098931 UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Enterobacter sp. SENG-6
25% identity, 92% coverage
4e7bA / P33038 E. Cloacae mura in complex with udp-glucose
25% identity, 92% coverage
- Ligand: uridine-5'-diphosphate-glucose (4e7bA)
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 789,361 different protein sequences to 1,256,019 scientific articles. Searches against EuropePMC were last performed on January 10 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory