PaperBLAST
PaperBLAST Hits for CharProtDB::CH_123431 N-acetylglucosamine kinase (Candida albicans) (493 a.a., MTETSISGLR...)
Show query sequence
>CharProtDB::CH_123431 N-acetylglucosamine kinase (Candida albicans)
MTETSISGLRGPKSMYFMEIVDVSSQESSVLSSIVESFTSAVSASNLGVYSDEVLCDIKS
SLKENSPITMLPNYNVSPTGDEHGQYLVIDLGGSTLRIAVVDISKPHPNLSRSERITIVV
EKSWIIGNDFKRIDGEFFKYIGSKINEILMGQNVIDVKSVINTGITWSFPLETTDYNRGK
IKHVSKGYTVGEDIYDKDLKMVLEDTLRQEYGLTLDVQSILNDSLAVYSAGCFIDSKMKL
AMVLGTGINMCCSLKRSSDIHPSKMLADATLFNCELSLFGQNLCKDFATKYDIIIDKRFA
GFSHHFKTFMEPDPITKTLFQPHELMTSGRYLPELTRLVVVDLIEAGEIFQNVDHQQMYQ
EYGGFSGELMCFVHENDDYDDIHDKLCKAYGWTTVGLSDIVCLKEVVSCIIKRAAFIVAN
AIIAFFKLLGSDELGGDVTIGYVGSVLNYFHKYRRLIVEYVNNAEEAKGIKVDLKLIENS
SIIGAAIGAAYHK
Running BLASTp...
Found 301 similar proteins in the literature:
HXK1 N-acetylglucosamine kinase from Candida albicans (see 3 papers)
100% identity, 100% coverage
- CharProtDB CGD description: N-acetylglucosamine (GlcNAc) kinase; involved in GlcNAc utilization; required for wild-type hyphal growth and mouse virulence; gene expression is GlcNAc-induced; induced in response to alpha pheromone in SpiderM medium
HXK1_CANAL / Q59RW5 N-acetylglucosamine kinase 1; GlcNAc kinase 1; Hexokinase 1; EC 2.7.1.59; EC 2.7.1.1 from Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) (see 10 papers)
XP_712429 hexokinase 1 from Candida albicans SC5314
99% identity, 100% coverage
- function: Component of the N-acetylglucosamine catabolic cascade that phosphorylates N-acetylglucosamine (GlcNAc), and allows the unique ability to utilise GlcNAc as carbon source. Converts GlcNAc to GlcNAc- 6-P. Also able to phosphorylate glucose, glucosamine (GlcN), and mannose. Galactose, fructose, N-acetylmannosamine (ManNAc), mannosamine (ManN), galactosamine (GalN), and N-acetylgalactosamine (GalNAc) are not phosphorylated by HXK1. GlcNAc metabolism is closely associated with virulence and morphogenesis, and is involved in the cell wall synthesis. Acts both as a repressor and an activator of genes involved in maintaining cellular homeostasis. Contributes to white-opaque morphological transition and plays a role as a filamentation repressor.
catalytic activity: N-acetyl-D-glucosamine + ATP = N-acetyl-D-glucosamine 6- phosphate + ADP + H(+) (RHEA:17417)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
catalytic activity: D-glucosamine + ATP = D-glucosamine 6-phosphate + ADP + H(+) (RHEA:10948)
subunit: Interacts with histone deacetylase SIR2 under filamentation- inducing conditions.
disruption phenotype: Greatly retards the growth of cells using GlcNAc as the sole carbon source, increases resistance against farnesol, and attenuates the virulence in a mouse systemic infection model. Leads to derepression of opaque specific gene expression, as well as to constitutive filamentous growth and hyperfilamentation in filamentation-inducing conditions. - Role of the N-acetylglucosamine kinase (Hxk1) in the regulation of white-gray-opaque tristable phenotypic transitions in C. albicans.
Cao, Fungal genetics and biology : FG & B 2016 (PubMed)- GeneRIF: Our findings suggest that the GlcNAc kinase, Hxk1, may function as a morphological regulator independent on its catabolic role in C. albicans.
AFUA_7G04040 hexokinase, putative from Aspergillus fumigatus Af293
26% identity, 81% coverage
- Effects of amino acids on the lignocellulose degradation by Aspergillus fumigatus Z5: insights into performance, transcriptional, and proteomic profiles
Miao, Biotechnology for biofuels 2019 - “...AFUA_5G07190, AFUA_6G08700, AFUA_6G14490, and AFUA_7G06140) in the proteome and transcriptome results remained consistent. Hexokinase (AFUA_2G05910, AFUA_7G04040), phosphoglucomutase (AFUA_3G11830), triosephosphate isomerase (AFUA_5G13450), phosphoglycerate mutase (AFUA_3G09290), phosphoglycerate kinase (AFUA_1G10350), enolase (AFUA_6G06770), and pyruvate kinase (AFUA_6G07430) also showed consistency between the two omics in the process of participating in...”
PADG_00393 uncharacterized protein from Paracoccidioides brasiliensis Pb18
28% identity, 69% coverage
- Transcriptional profiling of a fungal granuloma reveals a low metabolic activity of Paracoccidioides brasiliensis yeasts and an actively regulated host immune response
Borges, Frontiers in cellular and infection microbiology 2023 - “...domain transcription factor nscR DOWN(8) -1,00975 0,008026 Energy metabolism PADG_11844 aconitate hydratase DOWN(D) -1,52072 0,01042 PADG_00393 hexokinase [EC:2.7.1.1] DOWN(12) -1,38879 0,000134 PADG_03602 acyl-CoA dehydrogenase DOWN(D) -1,32085 0,000697 PADG_05290 Cytochrome b5 heme-binding domain-containing protein DOWN(D) -1,07453 0,005483 Cell cylce PADG_03022 PLK/PLK1 protein kinase DOWN(D) -1,25446 0,002207 PADG_11709...”
- “...energy metabolism were also repressed in the granulomatous yeasts, such as glucan 1,3-beta-glucosidase (PADG_07615), hexokinase (PADG_00393), acyl-CoA dehydrogenase (PADG_03602) and aconitate hydratase (PADG_11844). We also identified the downregulation of genes related to oxidative phosphorylation, such as cytochrome b5 (PADG_05290) and NADH dehydrogenase (ubiquinone) 1 alpha subcomplex...”
hxkC non-catalytic atypical hexokinase from Emericella nidulans (see paper)
28% identity, 72% coverage
- CharProtDB Description: Non-catalytic atypical hexokinase involved in regulation of extracellular proteases in response to carbon starvation; associates with mitochondria; Source:AspGD
An13g00510 uncharacterized protein from Aspergillus niger
26% identity, 85% coverage
- A Special Phenotype of Aconidial Aspergillus niger SH2 and Its Mechanism of Formation via CRISPRi
Yu, Journal of fungi (Basel, Switzerland) 2022 - “...semi-quantitative RT-PCR results indicated that the expression of hfbD (An08g09880), Ngt1 (An16g09020) An12g00480, and Hxk1 (An13g00510) reduced when the Dac1 (An16g09040) gene was restrained by the CRISPRi system ( Figure 5 E). Meanwhile, when the Dac1 (An16g09040) gene was restrained, the transformant grew slowly, and the...”
- “...A. After analysis, we found that the GlcNAc metabolic process genes, including Ngt1 (An16g09020), Hxk1 (An13g00510), and Dac1 (An16g09040), were greatly expressed in the spore-like propagule ( Table S12 ). Therefore, for the next study, we will focus on conidia, the MAPK signaling pathway, and GlcNAc...”
XP_504178 uncharacterized protein from Yarrowia lipolytica
27% identity, 90% coverage
TRIREDRAFT_79677 hexokinase from Trichoderma reesei QM6a
24% identity, 95% coverage
G4NB55 Phosphotransferase from Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
25% identity, 74% coverage
- The N-Acetylglucosamine Kinase from Yarrowia lipolytica Is a Moonlighting Protein.
Flores, International journal of molecular sciences 2021 - “...characterized [ 22 , 23 , 24 ]. In addition, a protein from Magnaporthe oryzae (G4NB55) annotated as a hexokinase-like enzyme in the databases but predicted to be a NAGA kinase by a phylogenetic analysis [ 19 ] was used. Table 1 shows the results of...”
- “...of the NAG genes could be separated. Expression of the putative kinase of M. oryzae (G4NB55) in the Ylnag5 mutant allowed growth in NAGA and completely repressed expression of the NAG genes in a glucose medium. These results identify this protein as a bona fide NAGA...”
CH_124038 putative hexokinase-1 [Neurospora crassa] from Magnaporthe grisea 70-15 (see paper)
25% identity, 75% coverage
TERG_03229 uncharacterized protein from Trichophyton rubrum CBS 118892
24% identity, 77% coverage
- The Antidepressant Sertraline Affects Cell Signaling and Metabolism in Trichophyton rubrum
Galvão-Rocha, Journal of fungi (Basel, Switzerland) 2023 - “...). This repressive effect on glycolysis pathway genes counteracts the initial step performed by hexokinase (TERG_03229), which is induced. Metabolic reprogramming induced the expression of glyoxylate cycle-associated genes (TERG_11639, TERG_01281, TERG_08288, and TERG_08287) and glycerol biosynthesis genes (TERG_07273, TERG_12172, and TERG_12173). Additionally, SRT modulated ethanol- and...”
- StuA-Regulated Processes in the Dermatophyte Trichophyton rubrum: Transcription Profile, Cell-Cell Adhesion, and Immunomodulation
Bitencourt, Frontiers in cellular and infection microbiology 2021 - “...2.20 2.66 1.93 TERG_03223 - N-acetylglucosamine-6-phosphate deacetylase -3.10 -3.04 TERG_03226 - glucosamine-6-phosphate deaminase -1.94 -2.08 TERG_03229 - hexokinase -1.65 -1.77 TERG_05576 - cell wall glucanase -1.61 TERG_05625 - class V chitinase -2.51 -2.13 -1.60 TERG_06189 - endo-1,3(4)-beta-glucanase -2.32 -1.99 TERG_06242 - glucanase -3.67 -3.14 TERG_06638 -...”
LOC105223186 hexokinase type 2 from Bactrocera dorsalis
28% identity, 71% coverage
Q9W330 Phosphotransferase from Drosophila melanogaster
NP_524848 hexokinase A, isoform A from Drosophila melanogaster
27% identity, 76% coverage
- Apocrine secretion in Drosophila salivary glands: subcellular origin, dynamics, and identification of secretory proteins
Farkaš, PloS one 2014 - “...Q27268 48.7 RNA splicing nuclear Heparan sulfate 2-O-sulfotransferase P25722 41.3 enzyme Golgi, membrane Hexokinase A Q9W330 59.2 metabolism cytoplasmic Histone acetyltransferase Tip60 Q960X4 61.2 transcription nuclear Hsp70/Hsp90 organizing protein Q9VPN5 55.7 co-chaperone cytoplasmic, endoplasmic reticulum Hsc70Cb Q9VUC1 88.5 co-chaperone cytoplasmic, endoplasmic reticulum IGF-II mRNA-binding protein Q8IR99...”
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...hexokinase (HK) domains from Macrobrachium nipponense (HK, KY270495), Litopenaeus vannamei (HK, ABO21409), Drosophila melanogaster (HKA-A, NP_524848), and Homo sapiens (GK 1, AAB97682). The amino acids implicated in glucose (G), glucose-6-phosphate (G6P), ATP, and Mg2+ binding sites are indicated in boxes. Figure 3 Phylogenetic tree based on...”
NP_001259384 hexokinase A, isoform C from Drosophila melanogaster
27% identity, 84% coverage
XP_002053418 hexokinase type 2 from Drosophila virilis
24% identity, 84% coverage
- Genomic signatures of globally enhanced gene duplicate accumulation in the megadiverse higher Diptera fueling intralocus sexual conflict resolution
Bao, PeerJ 2020 - “...XP_002050845, XP_002050869, XP_002051123, XP_002051268, XP_002051368, XP_002051379, XP_002051380, XP_002051381, XP_002051422, XP_002051833, XP_002051868, XP_002052164, XP_002052597, XP_002052598, XP_002052822, XP_002053418, XP_002053420, XP_002053421, XP_002053422, XP_002053434, XP_002053611, XP_002054324, XP_002054373, XP_002054903, XP_002055038, XP_002055293, XP_002055407, XP_002055451, XP_002056569, XP_002056572, XP_002056889, XP_002057022, XP_002057023, XP_002057025, XP_002057046, XP_002057047, XP_002057312, XP_002057775, XP_002057863, XP_002058101, XP_002058167, XP_002058764, XP_002059026, XP_002059558, XP_002059924, XP_002062810,...”
XP_004521010 hexokinase type 2 from Ceratitis capitata
27% identity, 70% coverage
- Genomic signatures of globally enhanced gene duplicate accumulation in the megadiverse higher Diptera fueling intralocus sexual conflict resolution
Bao, PeerJ 2020 - “...XP_002058101, XP_002058167, XP_002058764, XP_002059026, XP_002059558, XP_002059924, XP_002062810, XP_002065107, XP_003249498, XP_003250408, XP_003436731, XP_004518508, XP_004519116, XP_004519322, XP_004520566, XP_004521010, XP_004523446, XP_004523968, XP_004524766, XP_004526337, XP_004529383, XP_004529412, XP_004530158, XP_004531038, XP_004531266, XP_004533385, XP_004533719, XP_004535304, XP_004536134, XP_004536932, XP_004537692, XP_005187487, XP_005190203, XP_005190609, XP_006557645, XP_006558427, XP_006560286, XP_006563718, XP_006564634, XP_006564913, XP_006565977, XP_008191172, XP_008194238, XP_008199565, XP_008201416, XP_008201713,...”
XP_013192105 hexokinase type 2 isoform X2 from Amyelois transitella
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional...”
XP_026319325 hexokinase type 2 isoform X1 from Hyposmocoma kahamanoa
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...AdHK (ETN63660), AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure...”
XP_030568037 hexokinase type 2 from Drosophila novamexicana
26% identity, 76% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...numbers of different insect HKs are as follows: CmHK (MN612078), DaHK (XP_017870606), DnaHK (XP_030244412), DnoHK (XP_030568037), DbHK (XP_017853033), FoHK (XP_026290299), AsHK (KFB44789), AdHK (ETN63660), AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668),...”
XP_017870606 hexokinase type 2 from Drosophila arizonae
26% identity, 75% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...are clustered. GenBank accession numbers of different insect HKs are as follows: CmHK (MN612078), DaHK (XP_017870606), DnaHK (XP_030244412), DnoHK (XP_030568037), DbHK (XP_017853033), FoHK (XP_026290299), AsHK (KFB44789), AdHK (ETN63660), AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650),...”
XP_028166512 hexokinase type 2 isoform X3 from Ostrinia furnacalis
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C. medinalis were generated by SWISS-MODEL and...”
XP_022834977 hexokinase type 2 isoform X2 from Spodoptera litura
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...(XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C. medinalis were generated by SWISS-MODEL and visualized with PyMOL 2.3.4 [ 66 ]. Red represents -helices, yellow indicates -pleated...”
XP_021197237 hexokinase type 2 isoform X3 from Helicoverpa armigera
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C. medinalis were generated by SWISS-MODEL and visualized with PyMOL 2.3.4 [ 66 ]. Red represents -helices,...”
XP_021941686 LOW QUALITY PROTEIN: hexokinase type 2-like from Zootermopsis nevadensis
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...DnoHK (XP_030568037), DbHK (XP_017853033), FoHK (XP_026290299), AsHK (KFB44789), AdHK (ETN63660), AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396),...”
XP_004932650 hexokinase type 2 isoform X1 from Bombyx mori
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of...”
XP_030244412 hexokinase type 2 from Drosophila navojoa
26% identity, 75% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...GenBank accession numbers of different insect HKs are as follows: CmHK (MN612078), DaHK (XP_017870606), DnaHK (XP_030244412), DnoHK (XP_030568037), DbHK (XP_017853033), FoHK (XP_026290299), AsHK (KFB44789), AdHK (ETN63660), AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573),...”
XP_028033573 hexokinase type 2 isoform X2 from Bombyx mandarina
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions...”
PAS_chr3_1192 uncharacterized protein from Komagataella phaffii GS115
27% identity, 92% coverage
- Translational arrest due to cytoplasmic redox stress delays adaptation to growth on methanol and heterologous protein expression in a typical fed-batch culture of Pichia pastoris
Edwards-Jones, PloS one 2015 - “...glyceraldehyde-3-phosphate, significant changes in hexose metabolism were not expected. Therefore, transient induction of hexokinase (PAS_chr1-4_0447, PAS_chr3_1192, PAS_chr1-4_0561 and PAS_chr4_0624), phosphoglucomutase (PAS_chr 1-4_0264), glucose-6-phosphate dehydrogenase (PAS_chr 2-1_0308) suggest that during adaptation to growth on methanol, metabolic reserves of glycogen, trehalose and cell wall ,D glucans (see below)...”
- “...induced, consistent with the latter, while at 2h the relevant ( and ) hexokinases (PAS_chr14_0447, PAS_chr3_1192) were up-regulated in TRY1 -3 compared to GS115, indicating that the cells were trying to actively scavenge and import this glucose. By 4h, both GS115 and TRY1 -1 were recovering...”
XP_013138142 hexokinase type 2 isoform X4 from Papilio polytes
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C. medinalis were...”
XP_022115019 hexokinase type 2 isoform X4 from Pieris rapae
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C. medinalis were generated by SWISS-MODEL and visualized with...”
XP_017853033 hexokinase type 2 from Drosophila busckii
26% identity, 73% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...different insect HKs are as follows: CmHK (MN612078), DaHK (XP_017870606), DnaHK (XP_030244412), DnoHK (XP_030568037), DbHK (XP_017853033), FoHK (XP_026290299), AsHK (KFB44789), AdHK (ETN63660), AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584),...”
Q9NFT7 Hexokinase type 2 from Drosophila melanogaster
25% identity, 84% coverage
Hxk1 hexokinase 1; EC 2.7.1.1 from Entamoeba histolytica (see paper)
HXK1 / GI|1070151 hexokinase 1; EC 2.7.1.1 from Entamoeba histolytica (see paper)
EHI_098290 hexokinase from Entamoeba histolytica HM-1:IMSS
24% identity, 87% coverage
XP_008201713 hexokinase type 2 isoform X1 from Tribolium castaneum
24% identity, 84% coverage
- Genomic signatures of globally enhanced gene duplicate accumulation in the megadiverse higher Diptera fueling intralocus sexual conflict resolution
Bao, PeerJ 2020 - “...XP_004537692, XP_005187487, XP_005190203, XP_005190609, XP_006557645, XP_006558427, XP_006560286, XP_006563718, XP_006564634, XP_006564913, XP_006565977, XP_008191172, XP_008194238, XP_008199565, XP_008201416, XP_008201713, XP_019891905, XP_020713613, XP_021693932, XP_310951, XP_311387, XP_316164, XP_391836, XP_391836, XP_392478, XP_392899.2, XP_395280, XP_562185, XP_002056217, XP_966771, XP_967309,XP_967987, XP_968248,XP_969151, XP_969226, XP_969619, XP_970645, XP_971201, XP_971630, XP_972413, XP_972464, XP_973533, XP_975253, XP_EFA08711, AAEL002886, AAEL007001, AAEL007235, AAEL007392,...”
XP_030023668 hexokinase type 2 isoform X1 from Manduca sexta
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK...”
XP_026733584 hexokinase type 2 isoform X2 from Trichoplusia ni
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C. medinalis were generated by SWISS-MODEL and visualized with PyMOL 2.3.4 [ 66 ]. Red...”
XP_023938410 hexokinase type 2 isoform X4 from Bicyclus anynana
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C. medinalis were generated by SWISS-MODEL and visualized with PyMOL 2.3.4...”
XP_023727905 hexokinase type 2 isoform X5 from Cryptotermes secundus
24% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...DnaHK (XP_030244412), DnoHK (XP_030568037), DbHK (XP_017853033), FoHK (XP_026290299), AsHK (KFB44789), AdHK (ETN63660), AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410),...”
A0A0P0I1H5 hexokinase (EC 2.7.1.1) from Helicoverpa armigera (see paper)
25% identity, 84% coverage
XP_014362584 hexokinase type 2 isoform X4 from Papilio machaon
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C....”
XP_026496396 hexokinase type 2 isoform X4 from Vanessa tameamea
24% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C. medinalis were generated by SWISS-MODEL and visualized with PyMOL 2.3.4 [ 66...”
HXK2 / P04807 hexokinase 2 (EC 2.7.1.4; EC 2.7.1.1; EC 2.7.1.7) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 177 papers)
HXKB_YEAST / P04807 Hexokinase-2; Hexokinase PII; Hexokinase-B; EC 2.7.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
P04807 hexokinase (EC 2.7.1.1) from Saccharomyces cerevisiae (see 3 papers)
NP_011261 hexokinase 2 from Saccharomyces cerevisiae S288C
YGL253W Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene from Saccharomyces cerevisiae
25% identity, 70% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (PubMed:332086). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:332086).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
subunit: Homodimer - Comparative Analysis of Mass-Spectrometry-Based Proteomic Methods for Protein Target Discovery Using a One-Pot Approach.
Cabrera, Journal of the American Society for Mass Spectrometry 2020 - “...(P07280) NH NH NH H Pyruvate Decarboxylase (P06169) NH NH NH H Hexokinase-A; Hexokinase-B (P04806; P04807) NH NH H NH 40S ribosomal protein S15 (Q01855) NH NH H NH Cleavage factor IB (Q99383) NH H NH NH a Known CsA binding protein. Table 3. Summary of...”
- Protein kinase Ymr291w/Tda1 is essential for glucose signaling in saccharomyces cerevisiae on the level of hexokinase isoenzyme ScHxk2 phosphorylation*
Kaps, The Journal of biological chemistry 2015 - “...exhibiting a calculated molecular mass of 107 kDa (Uniprot P04807). The appearance of a protein band that corresponds within the analytical limits of native gel...”
- A model of yeast glycolysis based on a consistent kinetic characterisation of all its enzymes
Smallbone, FEBS letters 2013 - “...172000 HXK Glk1p P17709 136000 5.5 21100 HXK Hxk1p P04806 50500 1.1 40800 HXK Hxk2p P04807 185000 0.6 114000 PDC Pdc1p P06169 3220000 0.8 8970 PDC Pdc5p P16467 37200 19.1 471000 PDC Pdc6p P26263 19700 1.7 1520 PFK Pfk1p P16861 141000 0.7 89800 PFK Pfk2p P16862...”
- Sequence and structural analyses of nuclear export signals in the NESdb database
Xu, Molecular biology of the cell 2012 - “...HHT V RL I R V 117 + 1P65 CCCCCHHHHHHHHHH 0.52 0.60 NT Hxk2 120 P04807 304 MSSGYY L GE I LR L A L 318 + 1IG8 HHCHHHHHHHHHHHH 0.06 0.05 NT Topo II 121 P11388 1013 DTVLD I LRD F FE L R L 1027...”
- “...A IT L WQF L LQ L 14 + 1HBX CCCCHHHHCCCCC 0.32 0.23 Hxk2 120 P04807 19 VPKE L MQQ I EN F EK I 33 1IG8 CCHHHHHHHHHHHHH 0.39 0.22 Topo II g 121 P11388 1051 QARF I LEK I DGK I I I 1065 +...”
- Regulation of xylose metabolism in recombinant Saccharomyces cerevisiae
Salusjärvi, Microbial cell factories 2008 - “...2.0 2.9 2.2 P15992 Hsp26p_b YBR072W heat shock protein -0.6 -0.2 2.2 2.0 2.8 2.2 P04807 Hxk2p_a YGL253W hexokinase 2.6 2.9 10.7 -0.5 8.1 -3.4 P04807 Hxk2p_b YGL253W hexokinase 0.7 2.9 -1.1 -0.5 -1.8 -3.4 P28241 Idh2p YOR136W isocitrate dehydrogenase (NAD+) -0.9 -0.5 0.7 0.2 1.7...”
- Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.
Lesko, PLoS genetics 2023 - GeneRIF: Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.
- Snf1p/Hxk2p/Mig1p pathway regulates hexose transporters transcript levels, affecting the exponential growth and mitochondrial respiration of Saccharomyces cerevisiae.
Carrillo-Garmendia, Fungal genetics and biology : FG & B 2022 (PubMed)- GeneRIF: Snf1p/Hxk2p/Mig1p pathway regulates hexose transporters transcript levels, affecting the exponential growth and mitochondrial respiration of Saccharomyces cerevisiae.
- Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation.
Müller, Scientific reports 2022 - GeneRIF: Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation.
- Spontaneous mutations that confer resistance to 2-deoxyglucose act through Hxk2 and Snf1 pathways to regulate gene expression and HXT endocytosis.
Soncini, PLoS genetics 2020 - GeneRIF: Spontaneous mutations that confer resistance to 2-deoxyglucose act through Hxk2 and Snf1 pathways to regulate gene expression and HXT endocytosis.
- Reproductive Potential of Yeast Cells Depends on Overall Action of Interconnected Changes in Central Carbon Metabolism, Cellular Biosynthetic Capacity, and Proteostasis.
Maslanka, International journal of molecular sciences 2020 - GeneRIF: Reproductive Potential of Yeast Cells Depends on Overall Action of Interconnected Changes in Central Carbon Metabolism, Cellular Biosynthetic Capacity, and Proteostasis.
- Vma3p protects cells from programmed cell death through the regulation of Hxk2p expression.
Konarzewska, Biochemical and biophysical research communications 2017 (PubMed)- GeneRIF: Taken together, we have proposed a working model to describe how Vma3p protects cells against apoptosis through the regulation of HXK2 expression.
- Hexokinase 2 Is an Intracellular Glucose Sensor of Yeast Cells That Maintains the Structure and Activity of Mig1 Protein Repressor Complex.
Vega, The Journal of biological chemistry 2016 - GeneRIF: Hxk2 constitutes an intracellular glucose sensor that operates by changing its conformation in response to cytoplasmic glucose levels that regulate its interaction with Mig1 and thus its recruitment to the repressor complex of theSUC2promoter.
- Involvement of Aif1 in apoptosis triggered by lack of Hxk2 in the yeast Saccharomyces cerevisiae.
Amigoni, FEMS yeast research 2016 (PubMed)- GeneRIF: Deletion of AIF1 in hxk2Delta cells enhances survival after induction of apoptosis with both H2O2 and acetic acid, rescues the reduction of both growth rate and cell size, abrogates both H2O2 and acetic acid-induced ROS accumulation and decreases cell death, suggesting that Aif1 might be involved in both H2O2 and acetic acid-induced cell death in hxk2Delta cells.
- More
- Metabolic flux sampling predicts strain-dependent differences related to aroma production among commercial wine yeasts
Scott, Microbial cell factories 2021 - “...of secondary metabolites (Ehrlich pathway), Fatty acid degradation GLK1 HXK1 HXK2 EMI2 YLR446W YCL040W YFR053C YGL253W YDR516C Hexokinase (D-glucose:ATP) r_0534 Glycolysis, Gluconeogenesis, Fructose and mannose metabolism, Galactose metabolism, Amino sugar and nucleotide sugar metabolism, Carbon metabolism, Biosynthesis of secondary metabolites AAT2 YLR027C Aspartate transaminase r_0216 Alanine,...”
- Global analysis of Saccharomyces cerevisiae growth in mucin
Mercurio, G3 (Bethesda, Md.) 2021 - “...2.18e-08 3.60e-06 15 YJR105W ADO1 7.96 2.92e-07 1.45e-05 16 YBR131W CCZ1 7.94 7.46e-08 6.12e-06 17 YGL253W HXK2 7.89 6.13e-07 2.33e-05 18 YGR033C TIM21 7.88 7.36e-07 2.64e-05 19 YAR020C PAU7 7.80 2.65e-05 2.65e-04 20 YNR018W RCF2 7.79 1.80e-07 1.05e-05 21 YIL051C MMF1 7.59 3.62e-06 7.28e-05 22 YOR079C...”
- Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae
Cao, Microbial cell factories 2021 - “...subunit VIII 1.38 Glycolysis/gluconeogenesis YGR254W Eno1 Phosphopyruvate hydratase ENO1 1.52 YJL052W Tdh1 Glyceraldehyde-3-phosphate dehydrogenase 1.84 YGL253W Hxk2 Hexokinase 2 1.53 YDL021W Gpm2 Phosphoglycerate mutase family 1.41 YJR009C Tdh2 Glyceraldehyde-3-phosphate dehydrogenase 1.69 YGL062W Pyc1 Pyruvate carboxylase 1 1.31 Pentose-phosphate shunt, oxidative branch YHR183W Gnd1 Phosphogluconate dehydrogenase 1.64...”
- NoLogo: a new statistical model highlights the diversity and suggests new classes of Crm1-dependent nuclear export signals
Liku, BMC bioinformatics 2018 - “...in the 19ScNESs test set (These consisted of YBL105C, YDL207W, YDL229W, YER040W, YER165W, YFL039C, YGL071W, YGL253W, YJL128C, YJL187C, YKL012W, YLR074C, YLR347C, YPR093C, YPR119W; See Additionalfile 1 : Table S1 or Additionalfile 1 : Figure S1). Estimation of PSSM models for S. cerevisiae NESs For the PSSM...”
- The replicative lifespan-extending deletion of SGF73 results in altered ribosomal gene expression in yeast
Mason, Aging cell 2017 - “...168211 1955 285 YBL027W RPL19B Ribosomal 60S subunit protein L19B chrVII 23427 23542 1886.400024 451 YGL253W HXK2 Hexokinase 2 chrVII 254368 254492 1819.050049 211 YGL135W RPL1B Ribosomal 60S subunit protein L1B chrIV 321879 321984 1733.550049 295 YDL075W RPL31A Ribosomal 60S subunit protein L31A chrXIV 559344 559470...”
- Hexokinase plays a critical role in deoxynivalenol (DON) production and fungal development in Fusarium graminearum
Zhang, Molecular plant pathology 2016 (secret) - Systematic chemical-genetic and chemical-chemical interaction datasets for prediction of compound synergism
Wildenhain, Scientific data 2016 - “...heterochromatin Null mutant is viable, loss of silencing at telomeres, sensitive to many drugs S000005372 YGL253W HXK2 + HEX1|HKB|SCI2 Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and...”
- Protein kinase Ymr291w/Tda1 is essential for glucose signaling in saccharomyces cerevisiae on the level of hexokinase isoenzyme ScHxk2 phosphorylation*
Kaps, The Journal of biological chemistry 2015 - “...confirmed by adding the FASTA file of the Ygl253w amino acid sequence containing a serine-toalanine exchange at position 15 leaving all other settings...”
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XP_004520566 hexokinase type 2 from Ceratitis capitata
26% identity, 84% coverage
- Genomic signatures of globally enhanced gene duplicate accumulation in the megadiverse higher Diptera fueling intralocus sexual conflict resolution
Bao, PeerJ 2020 - “...XP_002057863, XP_002058101, XP_002058167, XP_002058764, XP_002059026, XP_002059558, XP_002059924, XP_002062810, XP_002065107, XP_003249498, XP_003250408, XP_003436731, XP_004518508, XP_004519116, XP_004519322, XP_004520566, XP_004521010, XP_004523446, XP_004523968, XP_004524766, XP_004526337, XP_004529383, XP_004529412, XP_004530158, XP_004531038, XP_004531266, XP_004533385, XP_004533719, XP_004535304, XP_004536134, XP_004536932, XP_004537692, XP_005187487, XP_005190203, XP_005190609, XP_006557645, XP_006558427, XP_006560286, XP_006563718, XP_006564634, XP_006564913, XP_006565977, XP_008191172, XP_008194238, XP_008199565, XP_008201416,...”
U3IVG9 hexokinase from Anas platyrhynchos platyrhynchos
25% identity, 48% coverage
AFUA_2G05910, Afu2g05910 hexokinase Kxk, putative from Aspergillus fumigatus Af293
24% identity, 84% coverage
- Proteomic characterization of Aspergillus fumigatus isolated from air and surfaces of the International Space Station
Blachowicz, Fungal genetics and biology : FG & B 2019 - “...compared to clinical isolates. At minimum twofold increased abundance of phosphoketolase (AFUA_3G00370) and hexokinase HxkA (AFUA_2G05910) 64 was observed. Among the decreased in abundance proteins were hydrolase Exg17 (AFUA_6G14490) 65 and 1,3-glucan modifying enzyme Sun1 (AFUA_7G05450) 66 . 4. DISCUSSION The proteome of A. fumigatus has...”
- “...Glucose-6-phosphate 1-epimerase 0.32 1.46 2.61 7.00E-05 AFUA_7G01830 Ugp1 UTP-glucose-1 -phosphate uridylyltransferase 0.00 1.35 2.11 4.43E-05 AFUA_2G05910 HxkA Hexokinase 0.01 1.20 2.12 8.68E-07 AFUA_7G05450 Sun1 Beta-1,3-glucan modifying enzyme 0.61 1.87 2.15 4.13E-02 AFUA_1G06910 Arabinogalactan endo-1,4-beta- galactosidase activity 0.43 1.53 2.04 9.05E-06 AFUA_6G05030 Polysaccharide deacetylase 0.09 1.21 1.23...”
- Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions
Blachowicz, Frontiers in microbiology 2019 - “...dehydrogenase GldB (AFUA_4G11730) ( Teutschbein et al., 2010 ), phosphoglycerate kinase (AFUA_1G10350), and hexokinase HxkA (AFUA_2G05910) ( Albrecht et al., 2010 ). Among the proteins involved in carbohydrate metabolism that exhibited decreased abundance were malate and alcohol dehydrogenases [AFUA_6G05210 ( Teutschbein et al., 2010 ), AFUA_5G06240...”
- “...AFUA_8G01490 1.32 1.64E-02 Cellobiohydrolase AFUA_4G11730 GldB 1.08 3.70E-02 Glycerol dehydrogenase AFUA_1G10350 1.03 4.74E-03 Phosphoglycerate kinase AFUA_2G05910 HxkA 1.01 1.07E-02 Hexokinase AFUA_1G06960 1.00 3.76E-02 Pyruvate dehydrogenase complex subunit alpha AFUA_6G05210 -1.18 1.36E-04 Malate dehydrogenase involved in the citric acid cycle AFUA_5G06240 AlcC -1.22 1.83E-04 Alcohol dehydrogenase AFUA_4G08410...”
- Effects of amino acids on the lignocellulose degradation by Aspergillus fumigatus Z5: insights into performance, transcriptional, and proteomic profiles
Miao, Biotechnology for biofuels 2019 - “...AFUA_1G17410, AFUA_5G07190, AFUA_6G08700, AFUA_6G14490, and AFUA_7G06140) in the proteome and transcriptome results remained consistent. Hexokinase (AFUA_2G05910, AFUA_7G04040), phosphoglucomutase (AFUA_3G11830), triosephosphate isomerase (AFUA_5G13450), phosphoglycerate mutase (AFUA_3G09290), phosphoglycerate kinase (AFUA_1G10350), enolase (AFUA_6G06770), and pyruvate kinase (AFUA_6G07430) also showed consistency between the two omics in the process of participating...”
- Transcriptomic and proteomic analyses of the Aspergillus fumigatus hypoxia response using an oxygen-controlled fermenter
Barker, BMC genomics 2012 - “...6-phosphate by hexokinase or glucokinase. There are three hexokinases annotated in the KEGG database (Afu2g00450, Afu2g05910, and Afu6g03980) in A. fumigatus , and these tended to be transcriptionally reduced or unchanged in response to hypoxia, whereas the two glucokinases (Afu6g02230 and Afu2g16330) were induced at later...”
- “...culture medium at later time-points. We chose to look at Afu6g02230 ( glkA ) and Afu2g05910 ( hxkA ) as these are thought to be the main active enzymes in A. fumigatus for this part of glycolysis [ 32 ]. As predicted by the microarray data,...”
- Integrative analysis of the heat shock response in Aspergillus fumigatus
Albrecht, BMC genomics 2010 - “...kinase PgkA) 0.12 0.42 1.08 0.80 No AFUA_1G13500 (transketolase TktA) 1.07 0.54 -0.62 -0.01 Yes AFUA_2G05910 (hexokinase Kxk) 1.39 1.27 0.35 0.73 Yes AFUA_2G10660 (mannitol-1-phosphate dehydrogenase) 1.70 1.67 1.09 1.46 No AFUA_3G08660 (isocitrate dehydrogenase Idp1) 1.19 0.95 0.08 0.42 Yes AFUA_4G04680 (FGGY-family carbohydrate kinase) 1.12 0.88...”
- “...transduction AFUA_1G05610 (protein phosphotase 2a 65 kd regulatory subunit) -0.60/0.88 (90) -0.54/0.96 (90) Carbohydrate metabolism AFUA_2G05910 (hexokinase Kxk) -0.95/0.91 (90) -0.83/0.96 (90) AFUA_2G10660 (mannitol-1-phosphate dehydrogenase) -0.13/0.51 (15) 0.03/0.57 (15) AFUA_3G08660 (isocitrate dehydrogenase Idp1) -0.76/0.91 (90) -0.77/0.93 (90) AFUA_4G11080 (acetyl-coenzyme A synthetase FacA) -0.79/0.97 (90) -0.71/0.93 (90)...”
XP_022677484 hexokinase 1 from Kluyveromyces marxianus DMKU3-1042
25% identity, 87% coverage
XP_013173406 hexokinase type 2 isoform X4 from Papilio xuthus
25% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK (XP_022834977). Figure 4 Three-dimensional structure of CmHK. Predictions for CmHK from C. medinalis were generated by...”
XP_002053421 hexokinase type 2 from Drosophila virilis
26% identity, 84% coverage
- Genomic signatures of globally enhanced gene duplicate accumulation in the megadiverse higher Diptera fueling intralocus sexual conflict resolution
Bao, PeerJ 2020 - “...XP_002051123, XP_002051268, XP_002051368, XP_002051379, XP_002051380, XP_002051381, XP_002051422, XP_002051833, XP_002051868, XP_002052164, XP_002052597, XP_002052598, XP_002052822, XP_002053418, XP_002053420, XP_002053421, XP_002053422, XP_002053434, XP_002053611, XP_002054324, XP_002054373, XP_002054903, XP_002055038, XP_002055293, XP_002055407, XP_002055451, XP_002056569, XP_002056572, XP_002056889, XP_002057022, XP_002057023, XP_002057025, XP_002057046, XP_002057047, XP_002057312, XP_002057775, XP_002057863, XP_002058101, XP_002058167, XP_002058764, XP_002059026, XP_002059558, XP_002059924, XP_002062810, XP_002065107, XP_003249498,...”
FGSG_08399 hexokinase-1 from Fusarium graminearum PH-1
25% identity, 81% coverage
3o5bA / P33284 Crystal structure of dimeric klhxk1 in crystal form vii with glucose bound (open state) (see paper)
23% identity, 86% coverage
- Ligand: beta-d-glucopyranose (3o5bA)
6jj7A / Q8LQ68 Crystal structure of oshxk6-glc complex
23% identity, 95% coverage
- Ligand: beta-d-glucopyranose (6jj7A)
HXK_KLULA / P33284 Hexokinase; EC 2.7.1.1 from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) (see paper)
P33284 hexokinase (EC 2.7.1.1) from Kluyveromyces lactis (see 2 papers)
23% identity, 86% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (PubMed:12882981). Has higher affinity for D-glucose (PubMed:12882981). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6- phosphate (PubMed:12882981).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
subunit: Monomer and homodimer. The monomeric form is active, the homodimeric form inactive.
CCM_06280 hexokinase from Cordyceps militaris CM01
23% identity, 98% coverage
- Metabolic comparison of aerial and submerged mycelia formed in the liquid surface culture of Cordyceps militaris
Suparmin, MicrobiologyOpen 2019 - “...and only CCM_08260 (4.182) was upregulated in the aerial mycelia. The expression of hexokinase (HK, CCM_06280), glyceraldehyde 3phosphate dehydrogenase (GAPDH, CCM_04549), and phosphoglycerate mutase (PGM, CCM_04218, CCM_09191) was also significantly upregulated following 12days of culture in the submerged mycelia, and the expression level was reduced at...”
- “...by oxidoreductase (CCM_01934), followed by alcohol dehydrogenase (ADH; CCM_09633), aldehyde dehydrogenase (ALDH; CCM_02203), hexokinase (HK; CCM_06280), alcohol dehydrogenase (ADH; CCM_02484), succinate dehydrogenase (SDH; CCM_07146), glucose6phosphate1dehydrogenase (G6PDH; CCM_06983), 5nucleotidase (5NT; CCM_00622), and adenylate cyclase, putative (ADCY; CCM_02396) in the submerged mycelia. In contrast, in the aerial mycelia,...”
XP_970645 hexokinase type 2 isoform X1 from Tribolium castaneum
24% identity, 89% coverage
- Genomic signatures of globally enhanced gene duplicate accumulation in the megadiverse higher Diptera fueling intralocus sexual conflict resolution
Bao, PeerJ 2020 - “...XP_310951, XP_311387, XP_316164, XP_391836, XP_391836, XP_392478, XP_392899.2, XP_395280, XP_562185, XP_002056217, XP_966771, XP_967309,XP_967987, XP_968248,XP_969151, XP_969226, XP_969619, XP_970645, XP_971201, XP_971630, XP_972413, XP_972464, XP_973533, XP_975253, XP_EFA08711, AAEL002886, AAEL007001, AAEL007235, AAEL007392, AAEL008128, AAEL009387, AAEL010777, AAEL013980, AEGA01006707, AEGA01013763, AEGA01014751, AEGA01022594, AEGA01022595, AEGA01030422, AGAP000565RA, AGAP002277, AGAP004002, AGAP004002, AGAP004657, AGAP004657, AGAP007871, AGAP009584, AGAP009833,...”
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...(EDX07186)), D. yakuba (HKA (EDX02859) and HKC (EDW91124)), Apis mellifera HKA-A (XP_392350), Tribolium castaneum (HK (XP_970645) and (XP_966410)), Aedes aegypti HK (AAU05129), Locusta migratoria (ACM78948), Glossina morsitans morsitans (ADD20426), and Culex quinquefasciatus (XP_001850122). Glucokinases: X. laevis (AAI70499), Oncorhynchus mykiss (NP_001117721), Sparus aurata (AAC33585), H. sapiens (GK1...”
HXK6_ORYSJ / Q8LQ68 Hexokinase-6; Hexokinase-2; EC 2.7.1.1 from Oryza sativa subsp. japonica (Rice) (see 2 papers)
Q8LQ68 hexokinase (EC 2.7.1.1) from Oryza sativa Japonica Group (see paper)
24% identity, 87% coverage
- function: Fructose and glucose phosphorylating enzyme (PubMed:16552590). Functions as a glucose sensor for plant growth and photosynthesis (PubMed:19010999).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825) - Comparative proteomics analyses of intraspecific differences in the response of <i>Stipa purpurea</i> to drought
Li, Plant diversity 2016 - “...Salt tolerant protein Triticum aestivum T P14009 0.63 620 14kDa proline-rich protein Daucus carota R Q8LQ68 0.54 158 Hexokinase-6-like Setaria italica D P20143 0.42 468 Photosystem I reaction center subunit VI, chloroplastic-like Solanum lycopersicum P Q9M573 0.62 87 60S ribosomal protein L31-like isoform 1 Brachypodium distachyon...”
HXKB_CANAL / P83776 Hexokinase-2; Cytoplasmic antigenic protein 3; Hexokinase PII; Hexokinase-B; EC 2.7.1.1 from Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) (see paper)
25% identity, 97% coverage
XP_011493158 hexokinase type 2 isoform X2 from Aedes aegypti
26% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...DaHK (XP_017870606), DnaHK (XP_030244412), DnoHK (XP_030568037), DbHK (XP_017853033), FoHK (XP_026290299), AsHK (KFB44789), AdHK (ETN63660), AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019),...”
M0R3X7 hexokinase from Rattus norvegicus
XP_038955090 hexokinase HKDC1 isoform X1 from Rattus norvegicus
26% identity, 49% coverage
B4E0J9 hexokinase from Homo sapiens
25% identity, 55% coverage
XP_026748941 hexokinase type 2 isoform X4 from Galleria mellonella
24% identity, 84% coverage
- Characterization, Knockdown and Parental Effect of Hexokinase Gene of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) Revealed by RNA Interference
Shakeel, Genes 2020 - “...AsHK (KFB44789), AdHK (ETN63660), AaHK (XP_011493158), CsHK (XP_023727905), ZnHK (XP_021941686), CqHK (XP_001850122), ApHK (ATA67117), GmHK (XP_026748941), HkHK (XP_026319325), AtHK (XP_013192105), BmHK (XP_004932650), BmaHK (XP_028033573), MsHK (XP_030023668), PmHK (XP_014362584), PpHK (XP_013138142), PxHK (XP_013173406), OfHK (XP_028166512), PrHK (XP_022115019), BaHK (XP_023938410), VtHK (XP_026496396), TnHK (XP_026733584), HaHK (XP_021197237), and SlHK...”
HKDC1_MOUSE / Q91W97 Hexokinase HKDC1; Hexokinase domain-containing protein 1; EC 2.7.1.1 from Mus musculus (Mouse) (see 4 papers)
NP_663394 hexokinase HKDC1 from Mus musculus
26% identity, 49% coverage
- function: Catalyzes the phosphorylation of hexose to hexose 6- phosphate, although at very low level compared to other hexokinases (By similarity). Has low glucose phosphorylating activity compared to other hexokinases (By similarity). Involved in glucose homeostasis and hepatic lipid accumulation (PubMed:30543855). Required to maintain whole-body glucose homeostasis during pregnancy; however additional evidences are required to confirm this role (PubMed:25648650, PubMed:27459389).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
disruption phenotype: Embryonic lethality. - HKDC1 promotes tumor immune evasion in hepatocellular carcinoma by coupling cytoskeleton to STAT1 activation and PD-L1 expression.
Zhang, Nature communications 2024 - GeneRIF: HKDC1 promotes tumor immune evasion in hepatocellular carcinoma by coupling cytoskeleton to STAT1 activation and PD-L1 expression.
- Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity.
Pusec, Scientific reports 2023 - GeneRIF: Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity.
- Proteomics identifies the developmental regulation of HKDC1 in liver of pigs and mice.
Martinez-Garza, American journal of physiology. Regulatory, integrative and comparative physiology 2023 - GeneRIF: Proteomics identifies the developmental regulation of HKDC1 in liver of pigs and mice.
- The Role of Hexokinase Domain Containing Protein-1 in Glucose Regulation During Pregnancy.
Zapater, Current diabetes reports 2021 - GeneRIF: The Role of Hexokinase Domain Containing Protein-1 in Glucose Regulation During Pregnancy.
- Hepatic HKDC1 Expression Contributes to Liver Metabolism.
Pusec, Endocrinology 2019 - GeneRIF: expression in hepatocytes results in defective mitochondrial function and altered hepatocellular metabolism
- Hepatic hexokinase domain containing 1 (HKDC1) improves whole body glucose tolerance and insulin sensitivity in pregnant mice.
Khan, Biochimica et biophysica acta. Molecular basis of disease 2019 - GeneRIF: Hepatic HKDC1 contributes to whole body glucose disposal, insulin sensitivity, and aspects of nutrient balance during pregnancy.
- Whole-exome sequencing revealed HKDC1 as a candidate gene associated with autosomal-recessive retinitis pigmentosa.
Zhang, Human molecular genetics 2018 - GeneRIF: Taken together, our data demonstrated that HKDC1 is associated with autosomal recessively inherited Retinitis pigmentosa.
G5B183 hexokinase from Heterocephalus glaber
25% identity, 47% coverage
XP_001386689 Hexokinase from Scheffersomyces stipitis CBS 6054
24% identity, 91% coverage
HKDC1_HUMAN / Q2TB90 Hexokinase HKDC1; Hexokinase domain-containing protein 1; EC 2.7.1.1 from Homo sapiens (Human) (see 4 papers)
Q2TB90 hexokinase (EC 2.7.1.1) from Homo sapiens (see paper)
NP_079406 hexokinase HKDC1 from Homo sapiens
25% identity, 47% coverage
- function: Catalyzes the phosphorylation of hexose to hexose 6- phosphate, although at very low level compared to other hexokinases (PubMed:30517626). Has low glucose phosphorylating activity compared to other hexokinases (PubMed:30517626). Involved in glucose homeostasis and hepatic lipid accumulation. Required to maintain whole-body glucose homeostasis during pregnancy; however additional evidences are required to confirm this role (By similarity).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825) - Pro-Inflammatory Characteristics of Extracellular Vesicles in the Vitreous of Type 2 Diabetic Patients.
Shan, Biomedicines 2024 - “...variable 24-1 TRBV24-1 0.008733 8 Q8WXI7 Mucin-16 MUC16 0.012206 9 O95171 Sciellin SCEL 0.024892 10 Q2TB90 Hexokinase HKDC1 0.025755 biomedicines-12-02053-t006_Table 6 Table 6 Proteins significantly lower in diabetic human vitreous samples. # Accession Number Protein Symbol p Value 1 P04406 Glyceraldehyde-3-phosphate dehydrogenase GAPDH 0.046 2 P12277...”
- Gramicidin S and Melittin - Potential anti-viral therapeutic peptides to treat SARS-CoV-2 infection
Ghalib, 2021 - Global proteome profiling of human livers upon ischemia/reperfusion treatment.
Cai, Clinical proteomics 2021 - “...1 OAS1 0.225 0.00002 Activate of latent RNase L and induce viral RNA degradation 2 Q2TB90 Hexokinase domain containing 1 HKDC1 0.361 0.00000 A member of the hexokinase involved in glucose metabolism 3 Q96AM1 MAS related GPR family member F MRGPRF 0.426 0.00006 Regulate nociceptor function...”
- Identification of p90 Ribosomal S6 Kinase 2 as a Novel Host Protein in HBx Augmenting HBV Replication by iTRAQ-Based Quantitative Comparative Proteomics
Yan, Proteomics. Clinical applications 2018 - “...AI APOA1 Lipid metabolism P02647 128 37756 22.5 0.586 17 Putative hexokinase HKDC1 Glycolysis transferase Q2TB90 103 123259 7.1 0.59 18 6phosphofructokinase, liver type PFKL Glycolysis transferase P17858 803 97322 24 0.6 19 Acylcoenzyme A thioesterase 9 ACOT9 AcylCoA metabolic process Q9Y305 198 54973 26.1 0.605...”
- Quantitative proteomics reveals that distant recurrence-associated protein R-Ras and Transgelin predict post-surgical survival in patients with Stage III colorectal cancer
Xu, Oncotarget 2016 - “...1.0 1.1 2.1 1.7 ND ND ND ND ND ND ND ND ND ND 49.2 Q2TB90 Putative hexokinase HKDC1 GN=HKDC1 2.4 ND 1.5 ND 1.0 1.0 2.4 1.9 ND ND ND ND ND ND ND ND ND ND 24.9 O14653 Golgi SNAP receptor complex member 2...”
- A quantitative proteomic analysis of long-term memory.
Rosenegger, Molecular brain 2010 - “...protein Q6BDS2 65.7 159.49 10 8 1.16 < 0.01 1.25 < 0.01 Putative hexokinase HKDC1 Q2TB90 53.72 102.52 2 2 1.19 0.01 1.39 < 0.01 Uncharacterized protein C2orf54 Q08AI8 49.33 49.57 11 31 1.12 0.05 1.16 0.01 Adenylate cyclase type 8 P40145 87.68 140.12 35 36...”
- CircVMP1 promotes glycolysis and disease progression by upregulating HKDC1 in colorectal cancer.
Chen, Environmental toxicology 2024 (PubMed)- GeneRIF: CircVMP1 promotes glycolysis and disease progression by upregulating HKDC1 in colorectal cancer.
- HKDC1 promotes tumor immune evasion in hepatocellular carcinoma by coupling cytoskeleton to STAT1 activation and PD-L1 expression.
Zhang, Nature communications 2024 - GeneRIF: HKDC1 promotes tumor immune evasion in hepatocellular carcinoma by coupling cytoskeleton to STAT1 activation and PD-L1 expression.
- Hepatitis B virus X protein promotes tumor glycolysis by downregulating lncRNA OIP5-AS1/HKDC1 in HCC.
Shi, Cellular signalling 2024 (PubMed)- GeneRIF: Hepatitis B virus X protein promotes tumor glycolysis by downregulating lncRNA OIP5-AS1/HKDC1 in HCC.
- HKDC1 promotes autophagy and proliferation in pancreatic adenocarcinoma through interaction with PARP1 and poly(ADP-ribosyl)ation.
Pang, Cellular signalling 2024 (PubMed)- GeneRIF: HKDC1 promotes autophagy and proliferation in pancreatic adenocarcinoma through interaction with PARP1 and poly(ADP-ribosyl)ation.
- HKDC1 reprograms lipid metabolism to enhance gastric cancer metastasis and cisplatin resistance via forming a ribonucleoprotein complex.
Zhao, Cancer letters 2023 (PubMed)- GeneRIF: HKDC1 reprograms lipid metabolism to enhance gastric cancer metastasis and cisplatin resistance via forming a ribonucleoprotein complex.
- HKDC1 in Gastric Cancer: A New Diagnostic, Prognostic Biomarker, and Novel Therapeutic Target.
Hu, Annals of clinical and laboratory science 2023 (PubMed)- GeneRIF: HKDC1 in Gastric Cancer: A New Diagnostic, Prognostic Biomarker, and Novel Therapeutic Target.
- The HOXC-AS2/miR-876-5p/HKDC1 axis regulates endometrial cancer progression in a high glucose-related tumor microenvironment.
Guo, Cancer science 2022 - GeneRIF: The HOXC-AS2/miR-876-5p/HKDC1 axis regulates endometrial cancer progression in a high glucose-related tumor microenvironment.
- Integrated Multiomics Reveals Glucose Use Reprogramming and Identifies a Novel Hexokinase in Alcoholic Hepatitis.
Massey, Gastroenterology 2021 - GeneRIF: Integrated Multiomics Reveals Glucose Use Reprogramming and Identifies a Novel Hexokinase in Alcoholic Hepatitis.
- More
LOTGIDRAFT_233221 hypothetical protein from Lottia gigantea
23% identity, 78% coverage
Q59TZ8 Phosphotransferase from Candida albicans (strain SC5314 / ATCC MYA-2876)
25% identity, 95% coverage
xprF hexokinase-like protein from Emericella nidulans (see 2 papers)
24% identity, 59% coverage
- CharProtDB Description: Non-catalytic hexokinase-like protein involved in regulation of extracellular proteases in response to carbon starvation; localized in nucleus; Source:AspGD
PGTG_18333 hexokinase from Puccinia graminis f. sp. tritici CRL 75-36-700-3
25% identity, 88% coverage
XP_006557645 hexokinase type 2 isoform X1 from Apis mellifera
24% identity, 82% coverage
- Genomic signatures of globally enhanced gene duplicate accumulation in the megadiverse higher Diptera fueling intralocus sexual conflict resolution
Bao, PeerJ 2020 - “...XP_004526337, XP_004529383, XP_004529412, XP_004530158, XP_004531038, XP_004531266, XP_004533385, XP_004533719, XP_004535304, XP_004536134, XP_004536932, XP_004537692, XP_005187487, XP_005190203, XP_005190609, XP_006557645, XP_006558427, XP_006560286, XP_006563718, XP_006564634, XP_006564913, XP_006565977, XP_008191172, XP_008194238, XP_008199565, XP_008201416, XP_008201713, XP_019891905, XP_020713613, XP_021693932, XP_310951, XP_311387, XP_316164, XP_391836, XP_391836, XP_392478, XP_392899.2, XP_395280, XP_562185, XP_002056217, XP_966771, XP_967309,XP_967987, XP_968248,XP_969151, XP_969226, XP_969619, XP_970645,...”
XP_001928917 putative hexokinase HKDC1 from Sus scrofa
24% identity, 48% coverage
A9U0F4 hexokinase (EC 2.7.1.1) from Physcomitrium patens (see paper)
24% identity, 82% coverage
A9RIW3 hexokinase (EC 2.7.1.1) from Physcomitrium patens (see paper)
22% identity, 82% coverage
B8LRJ7 Phosphotransferase from Picea sitchensis
23% identity, 80% coverage
AT1G47840 HXK3 (HEXOKINASE 3); ATP binding / fructokinase/ glucokinase/ hexokinase from Arabidopsis thaliana
NP_175220 hexokinase 3 from Arabidopsis thaliana
23% identity, 84% coverage
- HXK, SnRK1, and TOR signaling in plants: Unraveling mechanisms of stress response and secondary metabolism
Eom, Science progress 2024 - “...locus) SnRK1 (Genomic locus) TOR (NCBI accession number) References Arabidopsis AtHXK1 (AT4G29130) AtHXK2 (AT2G19860) AtHXK3 (AT1G47840) AtHXL1 (AT1G50460) AtHXL2 (AT3G20040) AtHXL3 (AT4G37840) AtSnRK1.1 (AT3G01090) AtSnRK1.2 (AT3G29160) AtSnRK1.3 (AT5G39440) NP_175425.2 22 , 106 , 107 Rice OsHXK1 (LOC_Os07g26540.1) a OsHXK2 (LOC_Os05g45590.1) OsHXK3 (LOC_Os01g71320.1) OsHXK4 (LOC_Os07g09890.1) OsHXK5 (LOC_Os05g44760.1)...”
- Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis
Rudenko, Antioxidants (Basel, Switzerland) 2023 - “...glutathione At4g29130 Hexokinase 1, HXK1, ATHXK1, GIN2 Hexose phosphorylation activity At2g19860 Hexokinase 2, HXK2, ATHXK2 At1g47840 Hexokinase 3, HXK3 At4g24620 Phosphoglucose Isomerase, PGI, Glucose-6-phosphate isomerase Transformation of d-glucose-6-phosphate into d-fructose-6-phosphate At3g02570 At1g67070 Man-6-phosphate Isomerase, Phosphomannose isomerase, PMI D-mannose-6-P formation from d-fructose-6-phosphate [ 264 ] At2g45790 Phosphomannomutase,...”
- Effect of different drying techniques on rose (Rosa rugosa cv. Plena) proteome based on label-free quantitative proteomics
Yue-Han, Heliyon 2023 - “...cytochrome-c oxidase 0.06 At3g12260 A0A2P6SA49 Putative NADH: ubiquinone reductase (H (+)-translocating) 0.23 Carbon metabolism (ath01200) At1g47840 A0A2P6PZ58 Phoshphotransferase 3.57 FBA8 A0A2P6RBK8 Fructose-bisphosphate aldolase 2.77 FBA3 A0A2P6R1E9 Fructose-bisphosphate aldolase 2.58 ENO1 A0A2P6P3B3 Phosphopyruvate hydratase 2.67 SHM2 A0A2P6PG69 Serine hydroxymethyltransferase 3.08 rbcL A0A513X9G1 Ribulose bisphosphate carboxylase large chain...”
- Salt-Specific Gene Expression Reveals Elevated Auxin Levels in Arabidopsis thaliana Plants Grown Under Saline Conditions
Cackett, Frontiers in plant science 2022 - “...1.3 AT1G35910 TREHALOSE-6-PHOSPHATE PHOSPHATASE D (TPPD) 2.3 2.1 AT1G43160 ETHYLENE RESPONSIVE FACTOR113 (RAP2.6) 1.7 1.2 AT1G47840 HEXOKINASE 3 (HXK3) 1.4 1.2 AT1G70170 MATRIX METALLOPROTEINASE (MMP) 0.8 1.7 AT2G01900 INOSITOL POLYPHOSPHATE PHOSPHATIDYLINOSITOL 5-PHOSPHATASE9 (T5PTASE9) 1.9 1.7 AT2G02820 MYB DOMAIN PROTEIN 88 (MYB88) 1.2 0.5 AT2G15390 FUCOSYLTRANSFERASE 4...”
- 4D genetic networks reveal the genetic basis of metabolites and seed oil-related traits in 398 soybean RILs
Han, Biotechnology for biofuels and bioproducts 2022 - “...[ 114 ] GmHXK New mQTL-O30 17 Marker169306, Marker169306 1.34 2.58 6.82 Oxalacetic acid Glyma17g37720 AT1G47840 gmx00500 Troncoso-Ponce et al. [ 115 ] GmAAPT New mQTL-O2 2 Marker1193792, Marker1193792 0.91~ 0.70 4.32~6.56 4.85~7.30 Pyruvate Glyma02g14211 AT1G13560 gmx00564 Bai et al. [ 16 ] GmFUM Known mQTL-O14...”
- An expanded role for the transcription factor WRINKLED1 in the biosynthesis of triacylglycerols during seed development
Kuczynski, Frontiers in plant science 2022 - “...thaliana the most likely candidate to be a WRI1 target is the plastidic isoform HXK3 (AT1G47840). We have identified an AW-box site upstream of this gene which is conserved in four of 12 species of this study ( Supplementary Table 7 ). However, this AW-site was...”
- Integrative Analysis of Transcriptomic and Proteomic Changes Related to Cytoplasmic Male Sterility in Spring Stem Mustard (Brassica juncea var. tumida Tsen et Lee)
Shen, International journal of molecular sciences 2022 - “...1 AT5G19220 UP DAP BjuA023577 K00703 Starch synthase, chloroplastic/amyloplastic AT5G24300 DOWN DAP BjuA037309 K00844 Phosphotransferase AT1G47840 DOWN DAP BjuB028137 K01810 Glucose-6-phosphate isomerase AT5G42740 UP DAP BjuA006306 K00134 Glyceraldehyde-3-phosphate dehydrogenase AT1G13440 DOWN DAP BjuA032999 K00927 Phosphoglycerate kinase AT1G79550 DOWN DAP BjuB022100 K00927 Phosphoglycerate kinase AT1G79550 DOWN DAP...”
- Transmembrane transport and stress response genes play an important role in adaptation of Arabidopsis halleri to metalliferous soils
Sailer, Scientific reports 2018 - “...Site-type AT1G18880 2351 35 2 Nitrate transporter 1.9/NFP2.9 SLC-mediated transmembrane transport 3.13 1.54 2.47 0.91 AT1G47840 2800 78 2 Hexokinase 3 SLC-mediated transmembrane transport 0.89 0.46 1.61 3.14 AT3G15380 3484 80 1 Choline transporter-like 1 SLC-mediated transmembrane transport 1.04 1.80 0.90 2.79 AT3G23550 2385 61 2...”
- More
- The plastid hexokinase pHXK: a node of convergence for sugar and plastid signals in Arabidopsis.
Zhang, FEBS letters 2010 (PubMed)- GeneRIF: plastid HXK enzyme activities, location, function in signal transduction, and cross talk to plastid GUN1 protein (a key signaling factor) were investigated. pHXK may be a node of convergence for sugar-mediated and PGE-derived signals.
NP_001096201 hexokinase-1 from Xenopus tropicalis
24% identity, 47% coverage
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...G. morhua HK1b (ABS89273), Danio rerio (AAH67330), Schizosaccharomyces pombe (CAA90848), Xenopus laevis (NP_001096656), Xenopus tropicalis (NP_001096201), Homo sapiens (AAH08730), Rattus norvegicus (NP_036866), Mus musculus (NP_001139572), and Bos taurus (AAA51661). Hexokinases 2: H. sapiens (AAH21116), R. norvegicus (NP_036867), M. musculus (AAH54472), D. rerio (AAH45496), and S. pombe...”
F7BA85 hexokinase from Equus caballus
24% identity, 47% coverage
FPSE_04405 hypothetical protein from Fusarium pseudograminearum CS3096
23% identity, 98% coverage
- Histone H3 N-Terminal Lysine Acetylation Governs Fungal Growth, Conidiation, and Pathogenicity through Regulating Gene Expression in Fusarium pseudograminearum
Jiang, Journal of fungi (Basel, Switzerland) 2024 - “...( Supplementary Tables S4S6 ) [ 38 ]. Additionally, dihydroxyacid dehydratase (FPSE_05147, FpILV3A ), hexokinase (FPSE_04405, FpHXK1 ), and acetohydroxyacid synthase (FPSE_00996, FpILV6 ), which play vital roles in growth, conidiation, and pathogenicity, are all downregulated in the FpH3 K9R , FpH3 K14R , and FpH3...”
CNBG_4382 hexokinase from Cryptococcus deuterogattii R265
24% identity, 76% coverage
Hxk2 hexokinase 2; EC 2.7.1.1 from Entamoeba histolytica (see paper)
HXK2 / GI|1070153 hexokinase 2; EC 2.7.1.1 from Entamoeba histolytica (see paper)
23% identity, 92% coverage
LOC105040520 hexokinase-2 from Elaeis guineensis
22% identity, 84% coverage
- Transcriptomic analysis of mesocarp tissue during fruit development of the oil palm revealed specific isozymes related to starch metabolism that control oil yield
Apriyanto, Frontiers in plant science 2023 - “...with oil yield are shown. These genes are EgSUT1 (LOC105041710), EgPGI (LOC105060694), EgFRK1.2 (LOC105035371), EgHK2.2 (LOC105040520), EgAPL1.1 (LOC105047182), EgAPS1.1 (LOC105060488), EgSS1 (LOC105040918), EgAMY3 (LOC105047709), EgBAM3 (LOC105043800), and EgAGL (LOC105059813). The (A) total starch, (B) sucrose, (C) maltotriose, and (D) maltose contents during fruit development are depicted...”
- “...that were positively correlated with oil yield are shown. These genes are EgFRK1.2 (LOC105035371), EgHK2.2 (LOC105040520), EgPGI (LOC105060694), EgPFK3.1 (LOC105040103), EgFBA1.1 (LOC105052993), EgFBA1.2 (LOC105059022), EgTPI1.1 (LOC105045658), EgG3PDH1.1 (LOC105034336), EgPGK3 (LOC105059872), EgPGAM1.1 (LOC105052340), EgENO2 (LOC105053561), and EgPK1.1 (LOC105038179). The abundances of (A) fructose and (B) glucose during...”
I1RAH1 hexokinase (EC 2.7.1.1) from Fusarium graminearum (see paper)
FGSG_00500 hexokinase from Fusarium graminearum PH-1
23% identity, 98% coverage
- Impact of Five Succinate Dehydrogenase Inhibitors on DON Biosynthesis of Fusarium asiaticum, Causing Fusarium Head Blight in Wheat
Xu, Toxins 2019 - “...pyruvic acid content. Here, we determined the relative expression levels of three key genes hexokinase (FGSG_00500), 6-phosphate fructokinase (FGSG_09456), and pyruvate kinase (FGSG_07528) in the glycolysis pathway. We found that fluopyram, flutolanil, boscalid, and fluxapyroxad caused down-regulated expression of hexokinase and 6-phosphate fructokinase but up-regulated the...”
- “...qRT-PCR for inter-reference actin Actin-qR TGCTTGGAGATCCACATTTG FGSG_00500-qF CGACCTCCACGACAACA qRT-PCR for determining the expression of hexokinase (FGSG_00500) FGSG_00500-qR GATAGCAGCAACGCCACA FGSG_09456-qF GATAGATTGGAGAGCCGAGAGA qRT-PCR for determining the expression of 6-phosphate fructokinase (FGSG_09456) FGSG_09456-qR GAGGTGCTGGATACACTTGATG FGSG_07528-qF GAGATCCGAACTGGTAAGACTC qRT-PCR for determining the expression of pyruvate kinase (FGSG_07528) FGSG_07528-qR CGTCAGAAGCGGTAGCATAA TRI1 -GFP-F...”
- Comparative acetylome analysis reveals the potential roles of lysine acetylation for DON biosynthesis in Fusarium graminearum
Zhou, BMC genomics 2019 - “...and development [ 28 ] GzZC230 FGSG_07133 Transcription factor DON, virulence [ 28 ] FgHXK1 FGSG_00500 Hexokinase DON, virulence and development [ 33 ] FgSKN7 FGSG_06359 Transcription factor DON, virulence and development [ 34 ] FaMyo2B FGSG_07469 Myosin protein Virulence and development [ 35 ] FgArb1...”
- Redox signalling from NADPH oxidase targets metabolic enzymes and developmental proteins in Fusarium graminearum
Fernando, Molecular plant pathology 2019 - “...as phosphoglycerate kinase (PGK, FGSG_03992), a hexokinase (FGSG_00500), a glucokinase (FGSG_08774), a pyruvate decarboxylase (FGSG_09834) and a pyruvate kinase...”
- “...(FGSG_07528) (Table S5). Genetic analyses have shown that FGSG_00500 is important for virulence and mycotoxin production in F. graminearum (Zhang et al., 2016)...”
- Hexokinase plays a critical role in deoxynivalenol (DON) production and fungal development in Fusarium graminearum
Zhang, Molecular plant pathology 2016 (secret)
HXK1_MOUSE / P17710 Hexokinase-1; Hexokinase type I; HK I; Hexokinase, tumor isozyme; EC 2.7.1.1 from Mus musculus (Mouse) (see 5 papers)
P17710 hexokinase (EC 2.7.1.1) from Mus musculus (see paper)
24% identity, 44% coverage
- function: Catalyzes the phosphorylation of various hexoses, such as D- glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6- phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
catalytic activity: D-glucosamine + ATP = D-glucosamine 6-phosphate + ADP + H(+) (RHEA:10948)
subunit: Monomer (By similarity). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (PubMed:23055941). Interacts with VDAC1. The HK1-VDAC1 complex interacts with ATF2 (By similarity). Interacts (via N-terminal spermatogenic cell-specific region) with PFKM isoform 2 and isoform 3 (via C-terminus) (PubMed:19889946). Interacts with SMAD5 (By similarity). - Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning
Dowling, European journal of translational myology 2024 - “...Protein name Gene Peptides Coverage (%) Molecular mass (kDa) (i) Glycolysis, gluconeogenesis and glycogen metabolism P17710 Hexokinase-1 Hk1 19 21.5 108.3 O08528 Hexokinase-2 Hk2 32 43.6 102.5 P47857 Phosphofructokinase, muscle Pfkm 32 54.6 85.3 P05064 Fructose-bisphosphate aldolase ALDOA Aldoa 37 89.3 39.4 P17751 Triosephosphate isomerase Tpi1...”
- Abnormal Regulation of Mitochondrial Sphingolipids during Aging and Alzheimer's Disease.
Crivelli, ASN neuro 2024 - “...of the following proteins: Q9D3D9, P02535, Q9DC61, P26645, Q99KE1, Q8VEM8, Q9Z1P6, P63040, Q91ZZ3, P19783, and P17710 which were analyzed for gene enrichment ( Figure 3B ). The main function of the individual proteins is reported in Supplementary Table 2 . The results of the analysis highlighted...”
- Proteomic Analysis Identifies Multiple Mechanisms of 5-Fluorouracil-Induced Gut Mucositis in Mice.
Ivanov, Cancers 2024 - “...Glutamate dehydrogenase 1, mitochondrial P26443 Glud1 2.0 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 P47856 Gfpt1 5.9 Hexokinase-1 P17710 Hk1 2.8 Hydroxymethylglutaryl-CoA synthase, mitochondrial P54869 Hmgcs2 8.6 Isocitrate dehydrogenase [NADP] cytoplasmic O88844 Idh1 2.5 Isocitrate dehydrogenase [NADP], mitochondrial P54071 Idh2 2.6 N-acetylneuraminic acid synthase (sialic acid synthase) Q99J77 Nans...”
- Micro-RNA and Proteomic Profiles of Plasma-Derived Exosomes from Irradiated Mice Reveal Molecular Changes Preventing Apoptosis in Neonatal Cerebellum.
Pazzaglia, International journal of molecular sciences 2022 - “...0.204 Q6ZWV3 60S ribosomal protein L10 Rpl10 0.210 P62267 40S ribosomal protein S23 Rps23 0.244 P17710 Hexokinase-1 Hk1 5.598 P19783 Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Cox4i1 6.534 Q64521 Glycerol-3-phosphate dehydrogenase, mitochondrial Gpd2 6.570 Q64314 Hematopoietic progenitor cell antigen CD34 Cd34 8.780 Q9Z126 Platelet...”
- Effects of semaglutide on vascular structure and proteomics in high-fat diet-induced obese mice.
Yue, Frontiers in endocrinology 2022 - “...regulatory subunit 1 Psmd1 0.8788 1.1211 Q8R0G9 Nuclear pore complex protein Nup133 Nup133 0.8849 1.1136 P17710 Hexokinase-1 Hk1 1.1147 0.9062 Q91VK4 Integral membrane protein 2C Itm2c 1.1441 0.8824 Q91WP0 Mannan-binding lectin serine protease 2 Masp2 1.1178 0.8794 Q921R8 Solute carrier family 41 member 3 Slc41a3 1.2734...”
- Search for Highly Divergent Tandem Repeats in Amino Acid Sequences
Rudenko, International journal of molecular sciences 2021 - “...Below, we present two examples of such sequences from Swiss-Prot. 1. Hexokinase-1 from Mus musculus (P17710; sequence length: 974 amino acids). The enzyme is the first in the glycolysis pathway, where it catalyzes the phosphorylation of hexoses; it consists of two sub-units and is ubiquitously expressed...”
- “...Multiple alignment of 64-character repeats found in the sequence of Mus musculus hexokinase-1 (P17710). No. Sequence of a Period 1 NMIMMTN..MNK.KN.MTINKNK.NKMNKKNIKMNKKTKNTMKTI.KKKTK.KKNN.IMKKMKNK.NMTM.NNNKNNT 2 NTKITNK..TNK.KM.KNKNN.K..ITMKKTN.KKTINTMMMKMT.MMKKKKININ.IKN.M.N..NMKT.TKKKKNI 3 NMMIMKNKKTKK.TKKMKKNMKK.MMMKTITK.NKKKKKNTNKKN.NNNNK.ITTN.NNT.K.K..KKKK.NKNNKNI 4 NTTKMMK..TKK.TK.TTKMNNK.NNTIKKIK.TTKNKTTKMNTI.TMTKT.MKNK.NKK.M.N..KITM.NKNK.NI 5 NKTKKMK..K.K.KM..NKMTNK.KITKMKNK.TKKNMKMINNNT.KKKKTNTMTKKNNK.M.TKKNMKKKTKNMTTN 6 KNKNMKN..NKK.NI.MNKNKKKKNKKKKKNM.KMTNMKNKMKMN.NKKKT.K.NKI.M.N..MKNI.NMMIMMN 7 KMMKKKTNT.KM.IKKNTNK.NKMKKKN.KNKKKIMKKT.NMMNK.TTNK.NMI.M.K..NMNKKNTKM 8 MMKMNTN..TNK.KM.MTNNNMK.NKMNKKNIKMNKKTKNTMKTI.KKKTK.KKNN.IMK.K.K..KMKM.NKMMMNK 9 TNMNMKI..NKKKTK.NNKNKTT.KITMKKNN.KNTIKTINKKMKKMNKKK.ININ.IKN.M.N..NNKT.NKKKKNI 10 NMKIMKN..TNK.KM.TKKNKKMTKK.MMMTTITK.TKKKKKNTNKKN.NNNNK.ITTK.NNT.K.K..KKKK.NKNNKNI 11...”
- Proteomic Analysis of Cardiac Adaptation to Exercise by High Resolution Mass Spectrometry
Al-Menhali, Frontiers in molecular biosciences 2021 - “...mitochondrial Acadm 160.0 5.1 0.0170 Q924X2 Carnitine O-palmitoyltransferase 1, muscle isoform Cpt1b 46.2 3.9 0.0189 P17710 Hexokinase-1 Hk1 33.7 5.5 0.0198 P51881 ADP/ATP translocase 2 Slc25a5 585.8 63.6 0.0200 Q8BH80 Vesicle-associated membrane protein, associated protein B and C Vapb 367.4 41.3 0.0198 Q9D6J6 NADH dehydrogenase [ubiquinone]...”
- PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming.
Feng, International journal of oncology 2020 - “...Slc2a1, Glut-1, Glut1 Solute carrier family 2, facilitated glucose transporter member 1 0.86 - 0.3308 P17710 Hk1 Hexokinase-1 0.91 - 0.1620 O08528 Hk2 Hexokinase-2 0.83 - 0.2196 Q3TRM8 Hk3 Hexokinase-3 NA NA NA Q8VDL4 Adpgk ADP-dependent glucokinase 0.87 - 0.2782 P06745 Gpi Glucose-6-phosphate isomerase 1.05 -...”
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A9S9F8 hexokinase (EC 2.7.1.1) from Physcomitrium patens (see paper)
22% identity, 81% coverage
D2GXH8 hexokinase (Fragment) from Ailuropoda melanoleuca
24% identity, 47% coverage
- Effects of Cryptorchidism on the Semen Quality of Giant Pandas from the Perspective of Seminal Plasma Proteomics
Qian, Genes 2024 - “...a proteinprotein interaction (PPI) network identified nine key proteins that may play crucial roles, including D2GXH8 (hexokinase Fragment), D2HSQ6 (protein tyrosine phosphatase), and G1LHZ6 (Calmodulin 2). Conclusions: We suspect that the high abundance of D2HSQ6 in cryptorchid individuals is associated with metabolic pathways, especially the insulin...”
- “...development and the species fertility [ 71 ]. The DEPs enriched by this pathway include D2GXH8 (hexokinase fragment) D2HSQ6, G1LH26 (Calmodulin 2), G1LYF2 (Fructose-bisphosphatase), G1MDP5 (Acetyl-CoA carboxylase), G1LEE1 (Hormone-sensitive lipase), G1M5J6 (Serine/threonine protein phosphatase), G1M3I9 (cAMP-dependent protein kinase), and G1MFD2 (hexokinase). Among them, D2HSQ6 was significantly...”
J9VWU8 Phosphotransferase from Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
24% identity, 77% coverage
G3UVV4 hexokinase from Mus musculus
24% identity, 47% coverage
hxkA hexokinase; EC 2.7.1.1 from Emericella nidulans (see paper)
24% identity, 84% coverage
- CharProtDB Description: Putative hexokinase with a predicted role in carbohydrate metabolism; Source:AspGD
A0A1D6MAK9 hexokinase (EC 2.7.1.1) from Zea mays (see paper)
23% identity, 87% coverage
NP_034568 hexokinase-1 isoform HK1-sb from Mus musculus
24% identity, 46% coverage
- Hemokinin-1 is a mediator of chronic restraint stress-induced pain.
Borbély, Scientific reports 2023 - GeneRIF: Hemokinin-1 is a mediator of chronic restraint stress-induced pain.
- A genome-wide CRISPR activation screen reveals Hexokinase 1 as a critical factor in promoting resistance to multi-kinase inhibitors in hepatocellular carcinoma cells.
Sofer, FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 (PubMed)- GeneRIF: A genome-wide CRISPR activation screen reveals Hexokinase 1 as a critical factor in promoting resistance to multi-kinase inhibitors in hepatocellular carcinoma cells.
- Hexokinase 1 cellular localization regulates the metabolic fate of glucose.
De, Molecular cell 2022 - GeneRIF: Hexokinase 1 cellular localization regulates the metabolic fate of glucose.
- Effect of Increased IL-1β on Expression of HK in Alzheimer's Disease.
Han, International journal of molecular sciences 2021 - GeneRIF: Effect of Increased IL-1beta on Expression of HK in Alzheimer's Disease.
- Fubp1 supports the lactate-Akt-mTOR axis through the upregulation of Hk1 and Hk2.
Kang, Biochemical and biophysical research communications 2019 (PubMed)- GeneRIF: Fubp1 upregulates the mRNA levels of two hexokinase genes, Hk1 and Hk2. We suggest that Fubp1 contributes to cell survival through supporting lactate-Akt-mTOR axis.
- Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism.
Lundgaard, Nature communications 2015 - GeneRIF: Hexokinase expression is highly enriched in neurons compared to astrocytes.
- The regulation of apoptosis by the downstream regulatory element antagonist modulator/potassium channel interacting protein 3 (DREAM/KChIP3) through interactions with hexokinase I.
Craig, Biochemical and biophysical research communications 2013 - GeneRIF: The interaction of DREAM with hexokinase I may be important in the regulation of neuronal apoptosis.
- The effect of standard chow and reduced hexokinase II on growth, cardiac and skeletal muscle hexokinase and low-flow cardiac ischaemia-reperfusion injury.
Smeele, Laboratory animals 2011 (PubMed)- GeneRIF: Standard chow affects body weight, heart weight and HK activity and HKI expression in the heart, without altering HKII expression.
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M0RAQ6 hexokinase from Rattus norvegicus
24% identity, 48% coverage
Q6GQU1 hexokinase from Mus musculus
NP_001139572 hexokinase-1 isoform HK1 from Mus musculus
24% identity, 47% coverage
- Proteomics analysis identifies phosphorylation-dependent alpha-synuclein protein interactions.
McFarland, Molecular & cellular proteomics : MCP 2008 - Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...(CAA90848), Xenopus laevis (NP_001096656), Xenopus tropicalis (NP_001096201), Homo sapiens (AAH08730), Rattus norvegicus (NP_036866), Mus musculus (NP_001139572), and Bos taurus (AAA51661). Hexokinases 2: H. sapiens (AAH21116), R. norvegicus (NP_036867), M. musculus (AAH54472), D. rerio (AAH45496), and S. pombe HK2 (CAA63488). Hexokinase 3: M. musculus (NP_001028417), R. norvegicus...”
V5KAK3 hexokinase (EC 2.7.1.1) from Branchiostoma japonicum (see paper)
24% identity, 89% coverage
XP_444978 hypothetical protein from Candida glabrata CBS 138
25% identity, 70% coverage
A0A0D3G422 Phosphotransferase from Oryza barthii
24% identity, 74% coverage
F1SUF2 hexokinase from Sus scrofa
24% identity, 47% coverage
- Proteomic Profiling Reveals Specific Molecular Hallmarks of the Pig Claustrum
Pirone, Molecular neurobiology 2023 - “...HSPH1 heat shock protein 70 family HSPH1 28 25 25 96,699 5.29 0.002 1.6 397 F1SUF2 Hexokinase HK1 36 33 24 83,569 6.96 0.016 1.4 407 F1RWX8 Ubiquitin-like modifier-activating enzyme 1 UBA1 15 16 16 117,757 5.55 0.00004 1.7 415 F1SDW6 Oxoglutarate dehydrogenase like OGDHL 26...”
- “...Ratio CLA/IN 345 F1S0N2 ATP-citrate synthase ACLY 21 11 11 57,558 7.12 0.025 2.1 397 F1SUF2 Hexokinase HK1 36 33 24 83,569 6.96 0.01 0.69 546 F1SCS1 DEAD-box helicase 1 DDX1 14 10 10 77,025 6.80 0.013 0.588 665 F1RRS3 Vesicle-fusing ATPase NSF 10 7 7...”
LOC107938100 hexokinase-2, chloroplastic from Gossypium hirsutum
25% identity, 93% coverage
NP_001309294 hexokinase-1 isoform a from Homo sapiens
23% identity, 46% coverage
- DDX24 promotes tumor progression by mediating hexokinase-1 induced glycolysis in gastric cancer.
Ni, Cellular signalling 2024 (PubMed)- GeneRIF: DDX24 promotes tumor progression by mediating hexokinase-1 induced glycolysis in gastric cancer.
- MiR-34a-HK1 signal axis retards bone marrow mesenchymal stem cell senescence via ameliorating glycolytic metabolism.
Sun, Stem cell research & therapy 2024 - GeneRIF: MiR-34a-HK1 signal axis retards bone marrow mesenchymal stem cell senescence via ameliorating glycolytic metabolism.
- Expanding the neurodevelopmental phenotype associated with HK1 de novo heterozygous missense variants.
Poole, European journal of medical genetics 2023 (PubMed)- GeneRIF: Expanding the neurodevelopmental phenotype associated with HK1 de novo heterozygous missense variants.
- Targeting hexokinase 1 alleviates NLRP3-mediated inflammation in apical periodontitis: A laboratory investigation.
Qian, International endodontic journal 2023 (PubMed)- GeneRIF: Targeting hexokinase 1 alleviates NLRP3-mediated inflammation in apical periodontitis: A laboratory investigation.
- Intermittent Hypoxia Promotes TAM-Induced Glycolysis in Laryngeal Cancer Cells via Regulation of HK1 Expression through Activation of ZBTB10.
Yang, International journal of molecular sciences 2023 - GeneRIF: Intermittent Hypoxia Promotes TAM-Induced Glycolysis in Laryngeal Cancer Cells via Regulation of HK1 Expression through Activation of ZBTB10.
- Hemokinin-1 is a mediator of chronic restraint stress-induced pain.
Borbély, Scientific reports 2023 - GeneRIF: Hemokinin-1 is a mediator of chronic restraint stress-induced pain.
- Proteomic analyses identify HK1 and ATP5A to be overexpressed in distant metastases of lung adenocarcinomas compared to matched primary tumors.
Pasternack, Scientific reports 2023 - GeneRIF: Proteomic analyses identify HK1 and ATP5A to be overexpressed in distant metastases of lung adenocarcinomas compared to matched primary tumors.
- Hexokinase-linked glycolytic overload and unscheduled glycolysis in hyperglycemia-induced pathogenesis of insulin resistance, beta-cell glucotoxicity, and diabetic vascular complications.
Rabbani, Frontiers in endocrinology 2023 - GeneRIF: Hexokinase-linked glycolytic overload and unscheduled glycolysis in hyperglycemia-induced pathogenesis of insulin resistance, beta-cell glucotoxicity, and diabetic vascular complications.
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F2DDR9 Phosphotransferase from Hordeum vulgare subsp. vulgare
23% identity, 92% coverage
HXKG_KLULA / Q6CUZ3 Glucokinase-1; Glucose kinase 1; GLK-1; Hexokinase GLK1; EC 2.7.1.2; EC 2.7.1.1 from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) (see paper)
Q6CUZ3 glucokinase (EC 2.7.1.2) from Kluyveromyces lactis (see paper)
25% identity, 88% coverage
- function: Glukokinase specific for aldohexoses. Phosphorylates glucose and mannose, but not fructose.
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
B6U4K6 Phosphotransferase from Zea mays
23% identity, 87% coverage
- Comparative Proteomic Analysis of the Response of Maize (Zea mays L.) Leaves to Long Photoperiod Condition
Wu, Frontiers in plant science 2016 - “...that Q9ZR52 (CK2 alpha) were downregulated at the three-leaf stage, as well as C0P8K7 (AtHXK1), B6U4K6 (AtHXK1) and B4F864 (NF-YB12; NF-YB13) in the six-leaf stage. Interestingly, the amount of B4FBL9 ( ELF5 ) decreased at both stages while B4FVS0 (ATFYPP3) and Q1A5Y4 (PHYB; PHYD) have no...”
- “...related to plant circadian rhythms have been identified. overexpression of Arabidopsis hexokinases (AtHXK1, C0P8K7, and B6U4K6) in tomato plants may reduce photosynthesis, inhibit growth and accelerate senescence (Dai et al., 1999 ), These results indicate that the activity of endogenous hexokinase is not a factor limiting...”
B4DG62 hexokinase from Homo sapiens
23% identity, 48% coverage
METBIDRAFT_36346 hypothetical protein from Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993
23% identity, 86% coverage
hxk / GI|134057515 hexokinase; EC 2.7.1.1 from Aspergillus niger (see paper)
hxk / CAA08922.1 hexokinase from Aspergillus niger (see paper)
An02g14380 hexokinase hxk-Aspergillus niger from Aspergillus niger
25% identity, 84% coverage
- Comprehensively dissecting the hub regulation of PkaC on high-productivity and pellet macromorphology in citric acid producing Aspergillus niger
Zheng, Microbial biotechnology 2022 - “...detail (Fig. 4 ). Along with the increase of pkaC expression, except hexokinase gene hxkA (An02g14380) and ribose 5phosphate isomerase gene rpiB , the expressions of most of the genes in the glycolysis and pentose phosphate pathway were upregulated, especially the two genes using glucose 6phosphate...”
- Genetic Interaction of Aspergillus nidulans galR, xlnR and araR in Regulating D-Galactose and L-Arabinose Release and Catabolism Gene Expression
Kowalczyk, PloS one 2015 - “...enzymes: XyrA = An01g03740, LadB = An16g01710, XhrA = An16g01650, SdhA = An07g01290, HxkA = An02g14380. Filamentous fungi use several pathways for D-galactose catabolism. The best studied pathway is the Leloir pathway, which is present in prokaryotic and eukaryotic organisms [ 14 ]. In A ....”
- Trancriptional landscape of Aspergillus niger at breaking of conidial dormancy revealed by RNA-sequencing
Novodvorska, BMC genomics 2013 - “...13.63 An09g03140 alcohol dehydrogenase 11.59 132.35 3.76 35.19 An16g05420 glucose-6-phosphate isomerase 11.61 23.29 2.44 9.54 An02g14380 hxkA , hexokinase 6.26 24.59 5.16 4.76 An18g01670 pfkA , 6-phosphofructokinase 64.25 42.44 12.52 3.38 An02g07470 fructose-bisphosphate aldolase 22.15 242.31 13.33 18.17 An08g02260 pgkA , phosphoglycerate kinase 19.01 32.17 15.68...”
HK1 / P19367 hexokinase-1 (EC 2.7.1.1) from Homo sapiens (see 7 papers)
HXK1_HUMAN / P19367 Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I; Hexokinase-A; EC 2.7.1.1 from Homo sapiens (Human) (see 17 papers)
23% identity, 47% coverage
- function: Catalyzes the phosphorylation of various hexoses, such as D- glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6- phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
catalytic activity: D-glucosamine + ATP = D-glucosamine 6-phosphate + ADP + H(+) (RHEA:10948)
subunit: Monomer (PubMed:10686099). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with VDAC1 (PubMed:22304920). The HK1-VDAC1 complex interacts with ATF2 (PubMed:22304920). Interacts (via N-terminal spermatogenic cell- specific region) with PFKM (via C-terminus) (By similarity). Interacts with SMAD5 (PubMed:28675158). - Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2
D'Ambrosio, Journal of neuroinflammation 2024 - “...31 P15104 GLUL, GLNS Glutamine synthetase 32 P09471 GNAO1 Guanine nucleotide-binding protein G(o) subunit 33 P19367 HK1 Hexokinase-1 34 P04908 H2AC4 Histone H2A type 1-B/E 35 P33778 H2BC3 Histone H2B type 1-B 36 P01762 IGHV3-11 Immunoglobulin heavy variable 311 37 P01614 IGKV2D-40 Immunoglobulin kappa variable 2D-40...”
- “...protein HSP 90- 23 P68871 HBB Hemoglobin subunit 24 P05546 SERPIND1 Heparin cofactor 2 25 P19367 HK1 Hexokinase-1 26 Q14764 MVP Major vault protein 27 Q02978 SLC25A11 Mitochondrial 2-oxoglutarate/malate carrier protein 28 P02686 MBP Myelin basic protein 29 P12882 MYH1 Myosin-1 30 Q9UKX2 MYH2 Myosin-2 31...”
- Explorative study of stimulated saliva proteome in head and neck cancer patients pre- and post-treatment
Almhöjd, Heliyon 2024 - “...Heterogeneous nuclear ribonucleoprotein K 1.100.21 0.780.19 0.030 P22626 Heterogeneous nuclear ribonucleoproteins A2/B1 1.570.63 0.820.42 0.039 P19367 Hexokinase-3 1.340.44 0.860.21 0.041 P0DOX5 Immunoglobulin gamma-1 heavy chain 1.000.25 0.590.21 0.022 Q7Z4W1 L-xylulose reductase 1.030.22 0.660.23 0.035 P26038 Moesin 1.160.38 0.690.20 0.026 P43490 Nicotinamide phosphoribosyltransferase 1.330.62 0.640.17 0.035 Q6XQN6...”
- Comparison of Microalgae <i>Nannochloropsis oceanica</i> and <i>Chlorococcum amblystomatis</i> Lipid Extracts Effects on UVA-Induced Changes in Human Skin Fibroblasts Proteome
Atalay, Marine drugs 2024 - “...(P00387), r = 0.98562; glutathione S-transferase P (P09211)galectin-1 (P09382), r = 0.97623; ATP-citrate synthase (P53396)hexokinase-1 (P19367), r = 0.97282; and peroxiredoxin-4 (Q13162)glutathione S-transferase omega-1 (P78417), r = 0.97046. On top of this, the proteins with the strongest negative correlation coefficients are as follows: signal transducer and...”
- “...C3 botulinum toxin substrate 1 0.87 0.21 0.12 3.08 0.48 1.06 0.79 0.35 0.80 0.33 P19367 Hexokinase-1 5.14 2.36 6.48 2.70 6.17 2.62 0.76 0.40 1.04 0.05 P60903 Protein S100-A10 0.78 0.36 0.56 0.83 0.32 1.65 0.48 1.07 1.48 0.56 Q9BTV4 Transmembrane protein 43 0.99 0.01...”
- MultifacetedProtDB: a database of human proteins with multiple functions.
Bertolini, Nucleic acids research 2024 - “...EC number (row Enzymes with only 1 EC in Table 2 ) is Hexokinase-1 (UniProt: P19367, gene: HK1). Its catalytic activity is described by EC: 2.7.1.1 (Hexokinase, Transferase class); as a second function it recognises bacterial peptidoglycans, playing a role in innate immunity and inflammation. It...”
- mTORC1-Driven Protein Translation Correlates with Clinical Benefit of Capivasertib within a Genetically Preselected Cohort of PIK3CA-Altered Tumors.
Sobsey, Cancer research communications 2024 - “...Glycogen synthase kinase-3 beta HADHB TP-Beta P55084 Trifunctional enzyme subunit beta, mitochondrial HK1 HK I P19367 Hexokinase-1 HNRNP-F hnRNP F P52597 Heterogeneous nuclear ribonucleoprotein F HNRNP-L hnRNP L P14866 Heterogeneous nuclear ribonucleoprotein L IGKC IGKC P01834 Immunoglobulin kappa constant IGKV4 IGKV4-1 P06312 Immunoglobulin kappa variable 41...”
- Integrating proteomics and explainable artificial intelligence: a comprehensive analysis of protein biomarkers for endometrial cancer diagnosis and prognosis.
Yasar, Frontiers in molecular biosciences 2024 - “...of proteins with accession Q01844 and high values of proteins with accession P04179, P07942, O00151, P19367 increase the risk of premenopausal in EC patients. However, in compliance with Figure 4B , the five most important proteins in predicting age are proteins with accession P04179, P07942, O00151,...”
- “...postmenopausal and premenopausal classification in endometrium cancer patients can be said P04179, P07942, O00151, Q01844, P19367. The expression of superoxide dismutase (P04179), which is directly related to tumor progression, invasion and angiogenesis and is synthesized by the SOD2 gene, is higher in premenopausal EC patients than...”
- Elucidating the impact of parthanatos-related microRNAs on the tumoral immune microenvironment and clinical outcome in low-grade gliomas.
Zhu, Discover oncology 2024 - “...N-Acetyltransferase 10 ENSG00000135372 Protein Coding Q9H0A0 47 GC11P034105 2.111518621 HK1 Hexokinase 1 ENSG00000156515 Protein Coding P19367 58 GC10P069269 2.005834579 NCF1 Neutrophil Cytosolic Factor 1 ENSG00000158517 Protein Coding P14598 55 GC07P084363 2.005834579 NAMPT Nicotinamide Phosphoribosyltransferase ENSG00000105835 Protein Coding P43490 56 GC07M106248 1.868104577 GPX4 Glutathione Peroxidase 4 ENSG00000167468...”
- First-in-Class Dual EZH2-HSP90 Inhibitor Eliciting Striking Antiglioblastoma Activity In Vitro and In Vivo.
Sharma, Journal of medicinal chemistry 2024 - “...OLA1 37.59 1.745 P24534 EEF1B2 75.42 1.792 O43242 PSMD3 36.87 1.801 Q15942 ZYX 24.18 1.903 P19367 HK1 88.39 1.921 P61204 ARF3 41.00 1.944 P07900 HSP90AA1 958.86 1.958 Q12792 TWF1 39.76 1.995 P11142 HSPA8 959.46 2.096 Q56VL3 OCIAD2 41.53 2.150 P50454 SERPINH1 113.69 2.186 O00299 CLIC1 74.34...”
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NP_036866 hexokinase-1 isoform 2 from Rattus norvegicus
24% identity, 47% coverage
- The HDAC Inhibitor Butyrate Impairs β Cell Function and Activates the Disallowed Gene Hexokinase I.
Bridgeman, International journal of molecular sciences 2021 - GeneRIF: The HDAC Inhibitor Butyrate Impairs beta Cell Function and Activates the Disallowed Gene Hexokinase I.
- α-Synuclein fibrillation products trigger the release of hexokinase I from mitochondria: Protection by curcumin, and possible role in pathogenesis of Parkinson's disease.
Dehghani, Biochimica et biophysica acta. Biomembranes 2020 (PubMed)- GeneRIF: alpha-Synuclein fibrillation products trigger the release of hexokinase I from mitochondria: Protection by curcumin, and possible role in pathogenesis of Parkinson's disease.
- Mitochondrial bound hexokinase type I in normal and streptozotocin diabetic rat retina.
Ramírez-Pérez, Mitochondrion 2020 (PubMed)- GeneRIF: Mitochondrial bound hexokinase type I in normal and streptozotocin diabetic rat retina.
- Mitochondria-Bound Hexokinase (mt-HK) Activity Differ in Cortical and Hypothalamic Synaptosomes: Differential Role of mt-HK in H2O2 Depuration.
Cavalcanti-de-Albuquerque, Molecular neurobiology 2018 (PubMed)- GeneRIF: specific roles of mitochondria bound-HK and SDH on the metabolism of each brain region, are reported.
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...(AAH67330), Schizosaccharomyces pombe (CAA90848), Xenopus laevis (NP_001096656), Xenopus tropicalis (NP_001096201), Homo sapiens (AAH08730), Rattus norvegicus (NP_036866), Mus musculus (NP_001139572), and Bos taurus (AAA51661). Hexokinases 2: H. sapiens (AAH21116), R. norvegicus (NP_036867), M. musculus (AAH54472), D. rerio (AAH45496), and S. pombe HK2 (CAA63488). Hexokinase 3: M. musculus...”
- In-silico and in-vitro investigation on the phenylalanine metabolites' interactions with hexokinase of Rat's brain mitochondria.
Ziamajidi, Journal of bioenergetics and biomembranes 2017 (PubMed)- GeneRIF: results suggest that phenylpyruvic acid is the cause of the reduction of glucose consumption by decreasing hexokinase activity. Therefore, production of ATP declines in brain cells.
- Up-regulation of hexokinase1 in the right ventricle of monocrotaline induced pulmonary hypertension.
Zhang, Respiratory research 2014 - GeneRIF: There was an increase in hexokinase1 expression in right ventricle from pulmonary hypertension rats.
- The application of selective reaction monitoring confirms dysregulation of glycolysis in a preclinical model of schizophrenia.
Martins-de-Souza, BMC research notes 2012 - GeneRIF: Data show statistically significant altered levels of Tpi1 and alteration of Hk1, Aldoc, Pgam1 and Gapdh with borderline significance in phencyclidine (PCP) rats compared to controls.
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HXK1_RAT / P05708 Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I; Hexokinase-A; EC 2.7.1.1 from Rattus norvegicus (Rat) (see 4 papers)
P05708 hexokinase (EC 2.7.1.1) from Rattus norvegicus (see 3 papers)
24% identity, 47% coverage
- function: Catalyzes the phosphorylation of various hexoses, such as D- glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6- phosphate, respectively) (PubMed:13211595, PubMed:3579310, PubMed:5871820). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:13211595, PubMed:5871820). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (By similarity). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (By similarity).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
catalytic activity: D-glucosamine + ATP = D-glucosamine 6-phosphate + ADP + H(+) (RHEA:10948)
subunit: Monomer (By similarity). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with VDAC1. The HK1-VDAC1 complex interacts with ATF2 (By similarity). Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity). Interacts with SMAD5 (By similarity). - Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma.
Yu, EBioMedicine 2022 - “...cell lines (ARP1 and RPMI8226), and the recombinant proteins for PSME3 (P03968, Solarbio) or PSME4 (P05708, Solarbio) were incubated with I3MO-D-biotin (200 M) at 4C overnight, respectively. Then, streptavidin MagBeads (#L00424, GenScript) 36 were added to cell lysates and rocked gently at room temperature for extra...”
- Myocardial proteomic profile in pulmonary arterial hypertension.
Hołda, Scientific reports 2020 - “...Myh7 Myosin-7 1.40 P60711 Actb Actin, cytoplasmic 1 1.19 P85108 Tubb2a Tubulin beta-2A chain 1.40 P05708 Hk1 Hexokinase-1 1.18 Q5XIE0 Anp32e Acidic leucine-rich nuclear phosphoprotein 32 family member E 1.35 Q5XFX0 Tagln2 Transgelin-2 1.17 P09006 Serpina3n Serine protease inhibitor A3N 1.35 Q63041 A1m Alpha-1-macroglobulin 1.17 P25235...”
- Investigation on Intestinal Proteins and Drug Metabolizing Enzymes in Simulated Microgravity Rats by a Proteomics Method.
Liu, Molecules (Basel, Switzerland) 2020 - “...(ALDH1A3) 3.37 0.0121 P07379 Cytosolic phosphoenolpyruvate carboxykinase (PCK1) 3.08 0.0122 P27881 Hexokinase-2 (HK2) 2.58 0.0214 P05708 Hexokinase-1 (HK1) 2.40 0.0031 P11884 mitochondrial Aldehyde dehydrogenase (ALDH2) 2.33 0.0005 P30839 Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 2.17 0.0005 P30835 ATP-dependent 6-phosphofructokinase, liver type (PFK) 0.49 0.0078 P00884...”
- Proteomic analysis of cortical neuronal cultures treated with poly-arginine peptide-18 (R18) and exposed to glutamic acid excitotoxicity
MacDougall, Molecular brain 2019 - “...Gapdh P04797 Glyceraldehyde-3-phosphate dehydrogenase 1.028 11.700 10.280 Gpi Q6P6V0 Glucose-6-phosphate isomerase 1.318 1.754 3.435 Hk1 P05708 Hexokinase-1 1.138 2.051 b 1.888 Ldha P04642 L-lactate dehydrogenase A chain 1.786 1.028 1.675 b Pkm P11980 Pyruvate kinase PKM 1.009 1.787 1.941 Taldo1 Q9EQS0 Transaldolase 1.259 1.837 2.109 Mitochondrial...”
- iTRAQ-Based Proteomics Analysis Reveals the Effect of Rhubarb in Rats with Ischemic Stroke.
Lin, BioMed research international 2018 - “...zeta/delta 77 1.90 45 P06761 Hspa5 57.41 55.20 78 kDa glucose-regulated protein 57 1.55 46 P05708 Hk1 76.09 47.17 Hexokinase-1 80 1.50 47 Q63198 Cntn1 77.28 48.29 Contactin-1 77 1.74 48 D3ZMI4 Epb41l1 82.1 34.24 Band 4.1-like protein 1 52 1.77 49 P09951 Syn1 91.64 72.30...”
- “...F1M378 rno04720: Long-term potentiation 4 0.004732 P62161, A0A0H2UHV6, P63086, P11275 rno01200: Carbon metabolism 4 0.025472 P05708, Q6P6R2, P14604, P08461 rno04722: Neurotrophin signaling pathway 4 0.028277 P61589, P62161, P63086, P11275 rno05020: Prion diseases 3 0.012162 P06761, A0A0G2K0M8, P63086 rno05031: Amphetamine addiction 3 0.044482 P62161, A0A0H2UHV6, P11275 rno05214:...”
- A Spectrum of Neural Autoantigens, Newly Identified by Histo-Immunoprecipitation, Mass Spectrometry, and Recombinant Cell-Based Indirect Immunofluorescence.
Scharf, Frontiers in immunology 2018 - “...ionotropic, delta-2 ( GRID2_RAT ) Q63226 61 164 36 114.0 7 Hexokinase-1 ( HXK1_RAT ) P05708 61 154 31 103.5 8 Homer protein homolog 3 ( HOME3_RAT ) Q9Z2X5 61 54 a 29 39.8 9 Inositol 1,4,5-trisphosphate receptor type 1 ( ITPR1_RAT ) P29994 61 357...”
- Proteomic Analysis of the Spatio-temporal Based Molecular Kinetics of Acute Spinal Cord Injury Identifies a Time- and Segment-specific Window for Effective Tissue Repair
Devaux, Molecular & cellular proteomics : MCP 2016 - “...number O35077 O35760 O88350 M0R590 P04905 F1LND7 P05544 P05708 P08009 P10860 P10959 P11030 P11980 P12346 P12785 P13233 P13668 G3V9G4 P17425 P24155 P24329 P28037...”
- “...Q5M7T5 Q5XI38 Q62669 Q68FY4 Q6MGC4 Q7TQ70 Cluster 7 P05708 P12346 P30904 Q6P502 D4ACB8 D4AEH9 F7F7H4 G3V721 Protein name Plectin Vimentin Transgelin Heat shock...”
- Changes in the Adult GluN2B Associated Proteome following Adolescent Intermittent Ethanol Exposure.
Swartzwelder, PloS one 2016 - “...2.08 0.00 Heat shock protein HSP 90-beta (HSP90AB1) P34058 36 73 2.03 0.00 Hexokinase-1 (HK1) P05708 37 59 1.59 0.03 Microtubule-associated protein 1A (MAP1A) P34926 5 19 3.80 0.00 Microtubule-associated protein 6 (MAP6) Q63560 2 17 8.50 0.00 Myelin basic protein S (Mbp) P02688 119 79...”
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A1Z0M7 hexokinase (EC 2.7.1.1) from Botrytis cinerea (see paper)
23% identity, 98% coverage
A0A3B5Z289 Phosphotransferase from Triticum aestivum
22% identity, 92% coverage
- Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in Wheat
Zhang, International journal of molecular sciences 2021 - “...phytocyanin domain-containing protein (A0A3B6HWQ3), Reticulon-like protein (A0A3B6A0V1), S-adenosylmethionine synthase (A0A3B6NSH7), Fn3_like domain-containing protein (A0A3B6DMR3), Phosphotransferase (A0A3B5Z289), Cinnamyl alcohol dehydrogenase (D7PGW03), and histone H2B (A0A3B6KCM0) proteins and their cognate genes showed exactly the same expression patterns in Y and P anthers, respectively. However, Mitochondrial fission 1 protein...”
- “...was carried out in related genes of sucrose synthase (A0A3B6JH89), sucrose-6F-phosphate phosphohydrolase SPP2 (Q9AXK5), Phosphotransferase (A0A3B5Z289), and starch synthase (B3V9H7) ( Table S9 ). Compared with the maintainer line, the decrease in Q9AXK5 was 0.097-fold, A0A3B6JH89 was 0.835-fold, A0A3B5Z289 was 0.088-fold, and B3V9H7 was 0.504-fold in...”
HXK1 / P04806 hexokinase 1 (EC 2.7.1.4; EC 2.7.1.1; EC 2.7.1.7) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 107 papers)
HXKA_YEAST / P04806 Hexokinase-1; Hexokinase PI; Hexokinase-A; EC 2.7.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
P04806 hexokinase (EC 2.7.1.1) from Saccharomyces cerevisiae (see 4 papers)
YFR053C Hexokinase isoenzyme 1, a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves the hexokinase Hxk2p from Saccharomyces cerevisiae
NP_116711 hexokinase 1 from Saccharomyces cerevisiae S288C
24% identity, 88% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (PubMed:332086). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:332086).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
subunit: Homodimer - The regulatory mechanism of the yeast osmoresponse under different glucose concentrations
Shen, iScience 2023 - “...Ypr1-GFP 6B 20 YPR160W Gph1-GFP 2M 3 YHR043C Dog2-GFP 7D 18 YOR185C Gsp2-GFP 11B 5 YFR053C Hxk1-GFP 4J 17 YLR113W Hog1-GFP 5A 4 msn2msn4 strain Hog1-GFP From Yihan Lins Lab https://www.nature.com/articles/s41467-021-23425-0 msn2msn4 strain Gpp1-GFP From Yihan Lins Lab msn2msn4 strain Hsp12-GFP From Yihan Lins Lab msn2msn4...”
- Physiological and Molecular Characterization of an Oxidative Stress-Resistant Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering
Kocaefe-Özşen, Frontiers in microbiology 2022 - “...STE12, SUA7, TEC1, XBP1 YIL057C RGI2 Respiratory growth induced 7.69 FKH2, MSN2, MSN4, RAP1, SPT10 YFR053C HXK1 HeXoKinase 7.63 BUR6, CYC8, GCN4, GCN5, HSF1, IXR1, MED2, MED4, MSN2, MSN4, RAP1, SFP1, SIN4, SPN1, SPT6, STP1, SUA7, TUP1, YAP1 YBR072W HSP26 Heat shock protein 7.32 HCM1, SNF2,...”
- DebaryOmics: an integrative -omics study to understand the halophilic behaviour of Debaryomyces hansenii
Navarrete, Microbial biotechnology 2022 - “...HXK1 Hexokinase1. Catalyses the phosphorylation of hexose, such as Dglucose and Dfructose, to hexose 6phosphate YFR053C TPS1 Alphatrehalosephosphate synthase. Catalyses the production of trehalose from glucose6phosphate YBR126C DEHA2E21956g NO NO TPS2 Trehalosephosphatase. Phosphatase catalytic subunit of the trehalose synthase complex that catalyses the production of trehalose...”
- Metabolic flux sampling predicts strain-dependent differences related to aroma production among commercial wine yeasts
Scott, Microbial cell factories 2021 - “...Biosynthesis of secondary metabolites (Ehrlich pathway), Fatty acid degradation GLK1 HXK1 HXK2 EMI2 YLR446W YCL040W YFR053C YGL253W YDR516C Hexokinase (D-glucose:ATP) r_0534 Glycolysis, Gluconeogenesis, Fructose and mannose metabolism, Galactose metabolism, Amino sugar and nucleotide sugar metabolism, Carbon metabolism, Biosynthesis of secondary metabolites AAT2 YLR027C Aspartate transaminase r_0216...”
- Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
Suarez-Diez, Scientific reports 2020 - “...), involved in cell separation 71 , and the genes YCL040W ( GLK1 ) and YFR053C ( HXK1 ), related to carbohydrate metabolic process. The above described genes, upregulated in the presence of lower (160mgL 1 ) and higher (800mgL 1 ) GN concentrations, are the...”
- A New Pathway for Mannitol Metabolism in Yeasts Suggests a Link to the Evolution of Alcoholic Fermentation
Gonçalves, Frontiers in microbiology 2019 - “...the construction of the hxk1 glk1 mutant, HXK1 and GLK1 from S. cerevisiae (YCL040W and YFR053C) were used to search for the respective orthologs in St. bombicola PYCC 5882 genome. A similar strategy as aforementioned was employed, starting with the construction of the glk1:ZEO mutant followed...”
- Transcriptional profiling reveals molecular basis and novel genetic targets for improved resistance to multiple fermentation inhibitors in Saccharomyces cerevisiae
Chen, Biotechnology for biofuels 2016 - “...YGR282C, YJL153C, YJR096W , YKL201C, YLL026W, YLR286C , YLR300W, YOL086C, YOR099W, YPL053C, YPR160W, YPR165W YEL040W, YFR053C, YGL156W, YGR043C, YGR256W, YJR096W , YLL026W , YLR286C , YML100W, YMR105C, YOR120W, YPR160W YBR001C, YBR105C, YCR005C, YDL055C, YER001W, YFL053W, YFR053C, YGL156W, YGL179C, YGR043C, YGR256W, YGR279C, YGR282C, YHR104W, YIL045W, YIL107C, YJL153C,...”
- Hexokinase plays a critical role in deoxynivalenol (DON) production and fungal development in Fusarium graminearum
Zhang, Molecular plant pathology 2016 (secret) - More
- Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation.
Müller, Scientific reports 2022 - GeneRIF: Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation.
- Comparative Analysis of Mass-Spectrometry-Based Proteomic Methods for Protein Target Discovery Using a One-Pot Approach.
Cabrera, Journal of the American Society for Mass Spectrometry 2020 - “...S19-A (P07280) NH NH NH H Pyruvate Decarboxylase (P06169) NH NH NH H Hexokinase-A; Hexokinase-B (P04806; P04807) NH NH H NH 40S ribosomal protein S15 (Q01855) NH NH H NH Cleavage factor IB (Q99383) NH H NH NH a Known CsA binding protein. Table 3. Summary...”
- biochem4j: Integrated and extensible biochemical knowledge through graph databases.
Swainston, PloS one 2017 - “...HXKA_YEAST) [ 7 ] String name Name String names Synonyms String[] uniprot Uniprot id (e.g. P04806) [ 7 ] String Reaction balance Flag indicating whether the reaction is balanced bool bigg.reaction BiGG Models id [ 17 ] String ec Enzyme Classification id String id Unique id...”
- Relationship between Metabolic Fluxes and Sequence-Derived Properties of Enzymes
Zikmanis, International scholarly research notices 2014 (no snippet) - A model of yeast glycolysis based on a consistent kinetic characterisation of all its enzymes
Smallbone, FEBS letters 2013 - “...1020000 GPM Gpm1p P00950 2200000 0.6 172000 HXK Glk1p P17709 136000 5.5 21100 HXK Hxk1p P04806 50500 1.1 40800 HXK Hxk2p P04807 185000 0.6 114000 PDC Pdc1p P06169 3220000 0.8 8970 PDC Pdc5p P16467 37200 19.1 471000 PDC Pdc6p P26263 19700 1.7 1520 PFK Pfk1p P16861...”
A9U189 hexokinase (EC 2.7.1.1) from Physcomitrium patens (see paper)
23% identity, 90% coverage
G7PF52 hexokinase from Macaca fascicularis
23% identity, 46% coverage
1qhaA / P19367 Human hexokinase type i complexed with atp analogue amp-pnp (see paper)
23% identity, 48% coverage
- Ligands: alpha-d-glucopyranose; 6-o-phosphono-alpha-d-glucopyranose; magnesium ion; phosphoaminophosphonic acid-adenylate ester (1qhaA)
G1MFD2 Hexokinase-2 from Ailuropoda melanoleuca
23% identity, 46% coverage
- Effects of Cryptorchidism on the Semen Quality of Giant Pandas from the Perspective of Seminal Plasma Proteomics
Qian, Genes 2024 - “...degree proteins included D2HSQ6 (protein tyrosine phosphatase, PTPs), G1LHU6 (Pyruvate kinase), G1MDP5 (Acetyl-CoA carboxylase), and G1MFD2 (hexokinase) ( Figure 5 A). Of these, only D2HSQ6 were highly expressed in the crytorchid group. In addition, we mapped the related proteins to the insulin signal pathway ( Figure...”
- “...G1MDP5 (Acetyl-CoA carboxylase), G1LEE1 (Hormone-sensitive lipase), G1M5J6 (Serine/threonine protein phosphatase), G1M3I9 (cAMP-dependent protein kinase), and G1MFD2 (hexokinase). Among them, D2HSQ6 was significantly upregulated in the seminal plasma of the cryptorchid group, indicating that the protein-related function was promoted in cryptorchidism. Earlier studies have shown that an...”
TERG_11518 uncharacterized protein from Trichophyton rubrum CBS 118892
24% identity, 38% coverage
P27595 Hexokinase-1 from Bos taurus
24% identity, 47% coverage
NP_001096656 hexokinase 1 L homeolog from Xenopus laevis
24% identity, 47% coverage
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...morhua HK1a (ABS89272), G. morhua HK1b (ABS89273), Danio rerio (AAH67330), Schizosaccharomyces pombe (CAA90848), Xenopus laevis (NP_001096656), Xenopus tropicalis (NP_001096201), Homo sapiens (AAH08730), Rattus norvegicus (NP_036866), Mus musculus (NP_001139572), and Bos taurus (AAA51661). Hexokinases 2: H. sapiens (AAH21116), R. norvegicus (NP_036867), M. musculus (AAH54472), D. rerio (AAH45496),...”
VDAG_04087 hexokinase from Verticillium dahliae VdLs.17
23% identity, 91% coverage
- Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae
Xiong, BMC genomics 2014 - “...MS formation (Additional file 12 : Figure S9). For example, two key enzyme encoding genes VDAG_04087 (hexokinase) and VDAG_01206 (pyruvate kinase) in glycolysis were significantly up-regulated during MS1-MS4 stages, indicative of an up-regulation of glycolysis during MS formation. Conversely, two genes that are critical for gluconeogensis...”
- “...Among the carbohydrate metabolic processes, several genes involved in energy production were enriched; such as VDAG_04087 (hexokinase), VDAG_01206 (pyruvate kinase), and VDAG_03029 (enolase). These results suggested that these differentially expressed genes are involved in a broad range of physiological functions, especially in proteolysis, protein modification, during...”
Q5W5U3 hexokinase (EC 2.7.1.1) from Bos taurus (see paper)
24% identity, 46% coverage
XP_024350444 Hexokinase from Echinococcus granulosus
25% identity, 87% coverage
EMI2_YEAST / Q04409 Putative glucokinase-2; Early meiotic induction protein 2; Glucose kinase 2; GLK-2; EC 2.7.1.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
YDR516C Emi2p from Saccharomyces cerevisiae
24% identity, 84% coverage
- function: Putative glucokinase involved in phosphorylation of aldohexoses and glucose uptake (By similarity). Involved in sporulation. Required for the full activation of the early meiotic inducer IME1.
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825) - Metabolic flux sampling predicts strain-dependent differences related to aroma production among commercial wine yeasts
Scott, Microbial cell factories 2021 - “...secondary metabolites (Ehrlich pathway), Fatty acid degradation GLK1 HXK1 HXK2 EMI2 YLR446W YCL040W YFR053C YGL253W YDR516C Hexokinase (D-glucose:ATP) r_0534 Glycolysis, Gluconeogenesis, Fructose and mannose metabolism, Galactose metabolism, Amino sugar and nucleotide sugar metabolism, Carbon metabolism, Biosynthesis of secondary metabolites AAT2 YLR027C Aspartate transaminase r_0216 Alanine, aspartate...”
- The Emi2 Protein of Saccharomyces cerevisiae is a Hexokinase Expressed under Glucose Limitation
Umekawa, Journal of applied glycoscience 2020 - “...enzymes. Analysis of the whole genome sequences of S. cerevisiae has revealed another hexokinase-like gene, YDR516C , with the standard name EMI2 . 7) However, there is no experimental evidence that the Emi2 protein possesses the enzyme activity for hexose phosphorylation. EMI2 was first identified as...”
- “...S. cerevisiae utilizes these three hexokinases for glycolysis. 4) However, there is another hexokinase-like gene, YDR516C , with the standard name EMI2 , in the S. cerevisiae genome. The ORF of EMI2 comprises 1,500 bp and encodes a protein of 500 amino acids. For comparison, we...”
- Identification of Genes in Saccharomyces cerevisiae that Are Haploinsufficient for Overcoming Amino Acid Starvation
Bae, G3 (Bethesda, Md.) 2017 - “...a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination M , C, v YDR516C EMI2 Nonessential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation M + , E, v YGL168W HUR1 Protein of unknown...”
- Canonical modeling of the multi-scale regulation of the heat stress response in yeast
Fonseca, Metabolites 2012 - “...(adapted from [ 1 ]). ESR * Genes Non-ESR* Paralogs Function HXK1 HXK2 Hexokinase GLK1 YDR516C Glucokinase PGM2 PGM1 Phosphoglucomutase PFK26 PFK27 2-phosphofructokinase FBP26 FBP1 Fructose-2,6-bisphosphatase GPM2 GPM1, GPM3 Phosphoglycerate mutase GSY2 GSY1 Glycogen synthase GLG1 GLG2 Glycogen initiator GND2 GND1 6-phosphogluconate dehydrogenase GPD1 GPD2 Glycerol...”
- Exploring the mode of action of antimicrobial peptide MUC7 12-mer by fitness profiling of Saccharomyces cerevisiae genomewide mutant collection
Lis, Antimicrobial agents and chemotherapy 2009 - “...DIP5 SNT309 Increased fitness YBL101C YJR056C YDR276C YDR516C YBL089W YDR067C YIL125W YDR346C YJR009C YDR310C YJR014W YJL110C YMR109W ECM21 YJR056C PMP3 EMI2...”
- Proteome-wide identification of proteins and their modifications with decreased ambiguities and improved false discovery rates using unique sequence tags
Shen, Analytical chemistry 2008 - “...YCL040W) cannot be distinguished from R.SAYLAAVPIA AILIK.T (from YDR516C) due to I and L being isobaric. Similarly, the peptide S.SSANK.L (from YKR092) cannot...”
- “...(from YCL040W) cannot be distinguished from R.SAYLAAVPIAAILIK.T (from YDR516C) as they have the same mass sequence due to I and L being isobaric. Use...”
- Transcriptome analysis of a respiratory Saccharomyces cerevisiae strain suggests the expression of its phenotype is glucose insensitive and predominantly controlled by Hap4, Cat8 and Mig1
Bonander, BMC genomics 2008 - “...transport YNR002C ATO2 3.8 b YKL217W JEN1 10.3 b YPL134C ODC1 6.3 b Transcriptional regulation YDR516C EMI2 3.6 b,c YEL066W HPA3 2.2 b Other YEL070W DSF1 9.4 b,c YGR243W FMP43 4.9 b The table is arranged as for Table 2. Factor changes denoted ' a '...”
- Selective inhibition of yeast regulons by daunorubicin: a transcriptome-wide analysis
Rojas, BMC genomics 2008 - “...YBL051C YMR074C AAT2 GPM1 PRB1 YDR453C YJU3 ARG8 ANB1 RPC40 YBL057C YMR085W ACO1 GPM2 PRY1 YDR516C YML056C ARO4 ARL1 RPG1 YBR012W-B YMR130W ADE12 GRE2 PRY3 YDR539W AYR1 BFR1 RPL13B YCL019W YMR158C-B ADE17 GRE3 PSA1 YFR017C CAR2 CAF20 RPL32 YCR082W YNL054W-B ADH1 GSF2 PST1 YGL121C CDC91 CBF5...”
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GLK1 / P17709 glucokinase (EC 2.7.1.1) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see paper)
HXKG_YEAST / P17709 Glucokinase-1; Glucose kinase 1; GLK-1; EC 2.7.1.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
6pdtA / P17709 Cryoem structure of yeast glucokinase filament (see paper)
NP_009890 glucokinase from Saccharomyces cerevisiae S288C
NP_009890, YCL040W Glk1p from Saccharomyces cerevisiae
23% identity, 86% coverage
- function: Two isoenzymes, hexokinase-1 and hexokinase-2, can phosphorylate keto- and aldohexoses in yeast, whereas a third isoenzyme, GLK, is specific for aldohexoses. All glucose phosphorylating enzymes are involved in glucose uptake.
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
subunit: Monomer - Ligands: alpha-d-glucopyranose; adenosine-5'-triphosphate (6pdtA)
- A model of yeast glycolysis based on a consistent kinetic characterisation of all its enzymes
Smallbone, FEBS letters 2013 - “...0.7 2610 FBA Fba1p P14540 4030000 1020000 GPM Gpm1p P00950 2200000 0.6 172000 HXK Glk1p P17709 136000 5.5 21100 HXK Hxk1p P04806 50500 1.1 40800 HXK Hxk2p P04807 185000 0.6 114000 PDC Pdc1p P06169 3220000 0.8 8970 PDC Pdc5p P16467 37200 19.1 471000 PDC Pdc6p P26263...”
- Polymerization in the actin ATPase clan regulates hexokinase activity in yeast.
Stoddard, Science (New York, N.Y.) 2020 - GeneRIF: Yeast containing nonpolymerizing Glk1 were less fit when growing on sugars and more likely to die when refed glucose. Glk1 polymerization arose independently from other actin-related filaments and may allow yeast to rapidly modulate glucokinase activity as nutrient availability changes.
- DebaryOmics: an integrative -omics study to understand the halophilic behaviour of Debaryomyces hansenii
Navarrete, Microbial biotechnology 2022 - “...Catalyses the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism YCL040W DEHA2E06556g NO NO HXK1 Hexokinase1. Catalyses the phosphorylation of hexose, such as Dglucose and Dfructose, to hexose 6phosphate YFR053C TPS1 Alphatrehalosephosphate synthase. Catalyses the production of trehalose from glucose6phosphate YBR126C...”
- Metabolic flux sampling predicts strain-dependent differences related to aroma production among commercial wine yeasts
Scott, Microbial cell factories 2021 - “...metabolism, Biosynthesis of secondary metabolites (Ehrlich pathway), Fatty acid degradation GLK1 HXK1 HXK2 EMI2 YLR446W YCL040W YFR053C YGL253W YDR516C Hexokinase (D-glucose:ATP) r_0534 Glycolysis, Gluconeogenesis, Fructose and mannose metabolism, Galactose metabolism, Amino sugar and nucleotide sugar metabolism, Carbon metabolism, Biosynthesis of secondary metabolites AAT2 YLR027C Aspartate transaminase...”
- Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
Suarez-Diez, Scientific reports 2020 - “...coding gene YLR286C ( CTS1 ), involved in cell separation 71 , and the genes YCL040W ( GLK1 ) and YFR053C ( HXK1 ), related to carbohydrate metabolic process. The above described genes, upregulated in the presence of lower (160mgL 1 ) and higher (800mgL 1...”
- A New Pathway for Mannitol Metabolism in Yeasts Suggests a Link to the Evolution of Alcoholic Fermentation
Gonçalves, Frontiers in microbiology 2019 - “...States). For the construction of the hxk1 glk1 mutant, HXK1 and GLK1 from S. cerevisiae (YCL040W and YFR053C) were used to search for the respective orthologs in St. bombicola PYCC 5882 genome. A similar strategy as aforementioned was employed, starting with the construction of the glk1:ZEO...”
- Identification of hexose kinase genes in Kluyveromyces marxianus and thermo-tolerant one step producing glucose-free fructose strain construction
Zhang, Scientific reports 2017 - “...72.4% identity with ScHXK2 (GenBank AAA34699). KmGLK1 (GenBank KX270228) shares 59.6% identity with ScGLK1 (GenBank NP_009890). To identify the function of hypothetical KmHXK1 or KmGLK1 , correspondent gene disrupted strains were constructed. After the disruption cassettes containing a ScURA3 gene were transformed into strain YHJ010 (...”
- Laboratory Evolution of a Biotin-Requiring Saccharomyces cerevisiae Strain for Full Biotin Prototrophy and Identification of Causal Mutations
Bracher, Applied and environmental microbiology 2017 - “...single-copy reference genes [YAL001C (TFC3), YBL015W (ACH1), YCL040W (GLK1), YDL029W (ARP2), YEL012W (UBC8), YER049W (TPA1), YBR196C (PGI1), YER178W (PDA1),...”
- Methods for causal inference from gene perturbation experiments and validation
Meinshausen, Proceedings of the National Academy of Sciences of the United States of America 2016 - “...18 19 20 Cause Effect SIE YMR104C YPL273W YCL040W YLL019C YMR186W YDR074W YMR173W YGR162W YOR027W YJL115W YOR153W YLR270W YOR153W YJL141C YAL059W YLR263W...”
- Transcriptional profiling reveals molecular basis and novel genetic targets for improved resistance to multiple fermentation inhibitors in Saccharomyces cerevisiae
Chen, Biotechnology for biofuels 2016 - “...(GO ID) Genes Annotated to the GO term Carbohydrate metabolic process (GO:0005975) YBR001C, YBR105C, YCL018W, YCL040W, YCR005C, YDL174C, YDL193W, YER062C, YFL053W, YGL134W, YGR254W, YHR046C, YHR174W, YJR096W, YKL201C, YLL026W, YML100W, YMR135C, YMR145C, YNR001C, YOL032W, YOL059W, YOL086C, YOL136C, YOR299W Response to chemical (GO:0042221) YBR006W, YBR101C, YCR021C, YDL124W, YDR135C,...”
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NP_001075245 hexokinase-2 from Equus caballus
23% identity, 46% coverage
HXK7_ORYSJ / Q1WM16 Hexokinase-7; Hexokinase-6; EC 2.7.1.1 from Oryza sativa subsp. japonica (Rice) (see 2 papers)
Q1WM16 hexokinase (EC 2.7.1.1) from Oryza sativa Japonica Group (see 2 papers)
22% identity, 74% coverage
- function: Fructose and glucose phosphorylating enzyme (PubMed:16552590). Functions in sugar signaling via a glycolysis- dependent manner under aerobic conditions, but its signaling role is suppressed when oxygen is deficient (PubMed:25951042).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825) - Carbon and nitrogen partitioning of transgenic rice T2A-1 (Cry2A*) with different nitrogen treatments
Ling, Scientific reports 2019 - “...2.84 1 1 1.755 0.003 Putative uncharacterized protein B8B9C4 55.4 1 15 1.719 0.004 Hexokinase-7 Q1WM16 8.42 2 3 1.660 0.007 Os08g0501132 protein (Fragment) A0A0P0XHL9 8.6 1 1 1.636 0.009 Putative uncharacterized protein B8A7D9 7.14 1 1 1.627 0.010 Putative uncharacterized protein B8BJ02 10.06 1 1...”
U3IR68 hexokinase from Anas platyrhynchos platyrhynchos
23% identity, 47% coverage
NP_989432 hexokinase-1 from Gallus gallus
23% identity, 47% coverage
HXK1_SPIOL / Q9SEK3 Hexokinase-1; SoHxK1; EC 2.7.1.1 from Spinacia oleracea (Spinach) (see paper)
Q9SEK3 hexokinase (EC 2.7.1.1) from Spinacia oleracea (see paper)
24% identity, 86% coverage
PADG_03813 hexokinase from Paracoccidioides brasiliensis Pb18
C1G977 Phosphotransferase from Paracoccidioides brasiliensis (strain Pb18)
24% identity, 84% coverage
- Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine
Almeida-Paes, Journal of fungi (Basel, Switzerland) 2020 - “...as some enzymes from glycolysis and citrate cycle. However, the key enzyme of glycolysis, hexokinase (PADG_03813), was less abundant in melanized cells of Pb 18 strain, as well as phosphofructokinase (PADG_00192 and PAAG_01583), in melanized cells of both strains studied ( Figure 2 ). Although the...”
- Differential Metabolism of a Two-Carbon Substrate by Members of the Paracoccidioides Genus
Baeza, Frontiers in microbiology 2017 - “...Pb EPM83, suggesting increased gluconeogenesis. Regulatory enzymes of glycolysis, phosphofructokinase-I (PAAG_01583; PABG_03640; PADG_00192) and hexokinase (PADG_03813), were repressed in Pb 01, Pb 03, Pb 339, and Pb EPM83. The reciprocal regulation of phosphofructokinase-I and fructose 1,6-biphosphatase clearly indicate increased gluconeogenesis and decreased glycolysis in the isolates...”
- Macrophage Interaction with Paracoccidioides brasiliensis Yeast Cells Modulates Fungal Metabolism and Generates a Response to Oxidative Stress
Parente-Rocha, PloS one 2015 - “...number 1 Protein description Score 2 Fold change 3 Glycolysis PADG_01896 Phosphoglycerate kinase 10933.7 0.20 PADG_03813 Hexokinase 1005.71 * PADG_00192 6-phosphofructokinase 1158.94 * PADG_00668 Fructose bisphosphate aldolase 19990.67 0.37 Protein synthesis PADG_04057 Translation initiation factor eIF3 4000.27 * PADG_00932 Translation initiation factor eIF3 478.38 * PADG_01891...”
- Extracellular vesicles from virulent P. brasiliensis induce TLR4 and dectin-1 expression in innate cells and promote enhanced Th1/Th17 response
Montanari, Virulence 2024 - “...UP(D) 6,671233 5,33E05 C1GBI8 Ribose-5-phosphate isomerase UP(D) 5,144232 3,44E05 C1G2P3 Enoyl-CoA hydratase UP(D) 4,382212 3,44E05 C1G977 Hexokinase UP (12S) 4,342947 0,032162 C1GCI8 2-methylcitrate synthase, mitochondrial UP(D) 3,832717 4,39E05 C1GL12 Glycogen debranching enzyme UP(D) 2,979608 0,000124 Amino acid metabolism C1G3Q0 Methylthioribulose-1-phosphate dehydratase UP(D) 4,193475 5,07E05 C1GF86 Cys-Gly...”
- “...of proteins related to energy metabolism, such as cytochrome C (C1GBI8), NADPH2:quinone reductase (C1GL50), hexokinase (C1G977), and mitochondrial 2-methylcitrate synthase (C1GCI8) were also found in the proteome of vEVs. We also detected a higher abundance of proteins in the vEVs that participate in amino acid metabolism...”
5hg1A / P52789 Crystal structure of human hexokinase 2 with cmpd 1, a c-2-substituted glucosamine (see paper)
23% identity, 49% coverage
- Ligands: 2-deoxy-2-{[(2e)-3-(3,4-dichlorophenyl)prop-2-enoyl]amino}-alpha-d-glucopyranose; 6-o-phosphono-beta-d-glucopyranose (5hg1A)
XP_017176890 hexokinase-2 isoform X1 from Mus musculus
23% identity, 54% coverage
E9Q5B5 Hexokinase-2 from Mus musculus
23% identity, 49% coverage
HXK2_MOUSE / O08528 Hexokinase-2; Hexokinase type II; HK II; EC 2.7.1.1 from Mus musculus (Mouse) (see 3 papers)
O08528 hexokinase (EC 2.7.1.1) from Mus musculus (see 2 papers)
NP_038848 hexokinase-2 from Mus musculus
23% identity, 48% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
subunit: Monomer (By similarity). Interacts with TIGAR; the interaction increases hexokinase activity in a hypoxia- and HIF1A-dependent manner (By similarity).
disruption phenotype: Embryonic lethality around E7.5 stage probably caused by the absence of hexokinase activity. - Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-beta signaling
Cummins, Biochimica et biophysica acta 2010 - “...NIDDM [ 51 56 ] NP_034404 glycerol-3-phosphate dehydrogenase 2 (GPD2) 4.95 NIDDM [ 57 ] NP_038848 hexokinase 2 (HK2) 5.28 Experimentally-induced diabetes, regulates D-glucose, regulated by glucose-6-phosphate [ 58 , 59 ] NP_001074 681 insulin receptor substrate 2 (IRS2) 9.64 Mediates effects of insulin [ 60...”
- Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning
Dowling, European journal of translational myology 2024 - “...Molecular mass (kDa) (i) Glycolysis, gluconeogenesis and glycogen metabolism P17710 Hexokinase-1 Hk1 19 21.5 108.3 O08528 Hexokinase-2 Hk2 32 43.6 102.5 P47857 Phosphofructokinase, muscle Pfkm 32 54.6 85.3 P05064 Fructose-bisphosphate aldolase ALDOA Aldoa 37 89.3 39.4 P17751 Triosephosphate isomerase Tpi1 13 67.9 26.7 P16858 Glyceraldehyde-3-phosphate dehydrogenase...”
- ISG15 Is Required for the Dissemination of Vaccinia Virus Extracellular Virions
Bécares, Microbiology spectrum 2023 - “...ribosomal protein L10-like Rpl10l 1.16 2.13 Q8BK63 Casein kinase I isoform alpha Csnk1a1 1.12 2.30 O08528 Hexokinase-2 Hk2 1.01 2.91 P62830 60S ribosomal protein L23 Rpl23 0.96 2.95 Q9D8E6 60S ribosomal protein L4 Rpl4 0.83 2.30 Q9QYI3 DnaJ homolog subfamily C member 7 Dnajc7 0.80 3.11...”
- Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia.
Wang, Experimental and therapeutic medicine 2022 - “...a Up Q8BJ03 COX15 Cytochrome c oxidase assembly protein COX15 homolog 1.878 0.017 a Up O08528 HK2 Hexokinase-2 1.912 0.015 a Up P48036 ANXA5 Annexin A5 1.951 0.049 a Up Q60994 ADIPOQ Adiponectin 1.951 0.031 a Up P07724 ALB Serum albumin 1.969 0.031 a Up P52503...”
- Comparative Proteomic Analysis of tPVAT during Ang II Infusion.
Liang, Biomedicines 2021 - “...protein 1 (Q64373), ACSVL (O35488), acetyl-CoA carboxylase 1 (Q5SWU9), 60S ribosomal protein L22 (P67984), hexokinase-2 (O08528), superoxide dismutase [Cu-Zn] (P08228), eukaryotic translation initiation factor 4E (P63073), high mobility group protein B1 (P63158), eukaryotic translation initiation factor 2 subunit 2 (Q99L45), lysine-specific histone demethylase 1A (Q6ZQ88), and...”
- “...hyaluronic acid receptor 1 P97452 BOP1 Ribosome biogenesis protein BOP1 Q8VE11 MTMR6 Myotubularin-related protein 6 O08528 HK2 Hexokinase-2 Q91V76 C11orf54 Ester hydrolase C11orf54 homolog Q9Z1T1 Ap3B1 AP-3 complex subunit beta-1 Q64008 Rab34 Ras-related protein Rab-34 Q920A5 SCPEP1 Retinoid-inducible serine carboxypeptidase P30993 C5aR1 C5a anaphylatoxin chemotactic receptor...”
- PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming.
Feng, International journal of oncology 2020 - “...2, facilitated glucose transporter member 1 0.86 - 0.3308 P17710 Hk1 Hexokinase-1 0.91 - 0.1620 O08528 Hk2 Hexokinase-2 0.83 - 0.2196 Q3TRM8 Hk3 Hexokinase-3 NA NA NA Q8VDL4 Adpgk ADP-dependent glucokinase 0.87 - 0.2782 P06745 Gpi Glucose-6-phosphate isomerase 1.05 - 0.2221 P12382 Pfkl ATP-dependent 6-phosphofructokinase, liver...”
- Does reversible cysteine oxidation link the Western diet to cardiac dysfunction?
Behring, FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 - Effects of cadmium and mercury on the upper part of skeletal muscle glycolysis in mice.
Ramírez-Bajo, PloS one 2014 - “...Molecular modelling analysis Models for the 3D structures of mouse HK, isoenzyme II (UniprotKb entry O08528) and PFK (UniprotKb entry P47857) where obtained from the SwissModel repository [32] using the template structures 2NZT (human HK II, 92% sequence identity) and 3O8L (rabbit muscle PFK, 96% sequence...”
- Peroxiredoxin 3 is a key molecule regulating adipocyte oxidative stress, mitochondrial biogenesis, and adipokine expression.
Huh, Antioxidants & redox signaling 2012 - More
CNH01400 hexokinase from Cryptococcus neoformans var. neoformans JEC21
24% identity, 77% coverage
XP_002059924 hexokinase type 2 from Drosophila virilis
24% identity, 89% coverage
- Genomic signatures of globally enhanced gene duplicate accumulation in the megadiverse higher Diptera fueling intralocus sexual conflict resolution
Bao, PeerJ 2020 - “...XP_002056572, XP_002056889, XP_002057022, XP_002057023, XP_002057025, XP_002057046, XP_002057047, XP_002057312, XP_002057775, XP_002057863, XP_002058101, XP_002058167, XP_002058764, XP_002059026, XP_002059558, XP_002059924, XP_002062810, XP_002065107, XP_003249498, XP_003250408, XP_003436731, XP_004518508, XP_004519116, XP_004519322, XP_004520566, XP_004521010, XP_004523446, XP_004523968, XP_004524766, XP_004526337, XP_004529383, XP_004529412, XP_004530158, XP_004531038, XP_004531266, XP_004533385, XP_004533719, XP_004535304, XP_004536134, XP_004536932, XP_004537692, XP_005187487, XP_005190203, XP_005190609, XP_006557645, XP_006558427,...”
HK2 / P52789 hexokinase-2 (EC 2.7.1.1) from Homo sapiens (see 4 papers)
HXK2_HUMAN / P52789 Hexokinase-2; Hexokinase type II; HK II; Hexokinase-B; Muscle form hexokinase; EC 2.7.1.1 from Homo sapiens (Human) (see 7 papers)
P52789 hexokinase (EC 2.7.1.1) from Homo sapiens (see 10 papers)
NP_000180 hexokinase-2 isoform 1 from Homo sapiens
23% identity, 46% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
subunit: Monomer (By similarity). Interacts with TIGAR; the interaction increases hexokinase activity in a hypoxia- and HIF1A-dependent manner (PubMed:23185017). - PDLIM1 interacts with HK2 to promote gastric cancer progression through enhancing the Warburg effect via Wnt/β-catenin signaling.
Lei, Cell and tissue research 2024 (PubMed)- GeneRIF: PDLIM1 interacts with HK2 to promote gastric cancer progression through enhancing the Warburg effect via Wnt/beta-catenin signaling.
- The effect and mechanism of hexokinase-2 on cisplatin resistance in lung cancer cells A549.
Xie, Environmental toxicology 2024 (PubMed)- GeneRIF: The effect and mechanism of hexokinase-2 on cisplatin resistance in lung cancer cells A549.
- A gain of function mutation in AKT1 increases hexokinase 2 and diminishes oxidative stress in meningioma.
Singh, Cytokine 2024 (PubMed)- GeneRIF: A gain of function mutation in AKT1 increases hexokinase 2 and diminishes oxidative stress in meningioma.
- Activation of the MEK/ERK Pathway Mediates the Inhibitory Effects of Silvestrol on Nasopharyngeal Carcinoma Cells via RAP1A, HK2, and GADD45A.
Yu, Frontiers in bioscience (Landmark edition) 2024 (PubMed)- GeneRIF: Activation of the MEK/ERK Pathway Mediates the Inhibitory Effects of Silvestrol on Nasopharyngeal Carcinoma Cells via RAP1A, HK2, and GADD45A.
- CCT6A facilitates lung adenocarcinoma progression and glycolysis via STAT1/HK2 axis.
Yu, Journal of translational medicine 2024 - GeneRIF: CCT6A facilitates lung adenocarcinoma progression and glycolysis via STAT1/HK2 axis.
- HK2 and LDHA upregulation mediate hexavalent chromium-induced carcinogenesis, cancer development and prognosis through miR-218 inhibition.
Wang, Ecotoxicology and environmental safety 2024 (PubMed)- GeneRIF: HK2 and LDHA upregulation mediate hexavalent chromium-induced carcinogenesis, cancer development and prognosis through miR-218 inhibition.
- Hexokinase 2 nonmetabolic function-mediated phosphorylation of IκBα enhances pancreatic ductal adenocarcinoma progression.
Tong, Cancer science 2024 - GeneRIF: Hexokinase 2 nonmetabolic function-mediated phosphorylation of IkappaBalpha enhances pancreatic ductal adenocarcinoma progression.
- RNA-binding protein SORBS2 increases human trophoblast cell migration via stabilizing HK2 mRNA in preeclampsia.
Song, Reproduction (Cambridge, England) 2024 (PubMed)- GeneRIF: RNA-binding protein SORBS2 increases human trophoblast cell migration via stabilizing HK2 mRNA in preeclampsia.
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- Impact of Nannochloropsis oceanica and Chlorococcum amblystomatis Extracts on UVA-Irradiated on 3D Cultured Melanoma Cells: A Proteomic Insight
Gęgotek, Cells 2024 - “...(copine-1 (Q99829), angiopoietin 4 (ANGPTL-4, Q9Y5C1), and 2-HS-glycoprotein (P02765)), apoptosis regulation (defensin 4A (O15263), hexokinase-2 (P52789)), proteolysis (proteasome 26S subunits 6 and 7 (H0YJC0, P35998)), and gene expression and cell proliferation (polyadenylate-binding protein 4 (PABPC4, Q13310), hypoxia-inducible factor 1-alpha (HIF 1, Q9NWT6), forkhead box 1 protein...”
- AAMP and MTSS1 Are Novel Negative Regulators of Endothelial Barrier Function Identified in a Proteomics Screen.
Podieh, Cells 2024 - “...0.0047 10,000 DAPK3 Death-associated protein kinase 3 O43293 autophagy, cytsoekeletal organization 0.0081 10,000 HK2 Hexokinase-2 P52789 glycolysis 0.0081 10,000 RPRD1B Regulation of nuclear pre-mRNA domain-containing protein 1B Q9NQG5 transcription 0.0081 10,000 PJA2 E3 ubiquitin-protein ligase Praja-2 O43164 ubiquitination 0.0081 10,000 ID3 DNA-binding protein inhibitor ID-3 Q02535...”
- Longitudinal Assessment of Nasopharyngeal Biomarkers Post-COVID-19: Unveiling Persistent Markers and Severity Correlations.
Redondo-Calvo, Journal of proteome research 2024 - “...GOGA3 Golgi subfamily A member 3 1.69 2.85 GOLGA3 Q08378 HXK2 hexokinase-2 3.20 2.87 HK2 P52789 AP3M1 AP-3 complex subunit mu-1 5.88 2.87 AP3M1 Q9Y2T2 DAG1 dystroglycan1 2.00 2.88 DAG1 Q14118 UGGG1 UDP-glucose:glycoprotein glucosyltransferase 1 2.80 2.88 UGGT1 Q9NYU2 GPD1L glycerol-3-phosphate dehydrogenase 1-like protein 3.59 2.88...”
- Exploration of the effect and mechanism of Scutellaria barbata D. Don in the treatment of ovarian cancer based on network pharmacology and in vitro experimental verification
Zhang, Medicine 2023 - “...Interferon regulatory factor 1 P10914 135 ERBB3 Receptor tyrosine-protein kinase erbB-3 P21860 136 HK2 Hexokinase-2 P52789 137 GSTM1 Glutathione S-transferase Mu 1 P09488 Figure 3. (A) Venn diagram shows ovarian cancer-related targets among the 4 databases. (B) Venn diagram of common intersecting targets genes in SBD...”
- Proteomic and computational analyses followed by functional validation of protective effects of trigonelline against calcium oxalate-induced renal cell deteriorations.
Peerapen, Computational and structural biotechnology journal 2023 - “...nuclear ribonucleoproteins A2/B1 O88569 HNRNPA2B1 252.84 41.1 13/13 37.40 101.202.34 92.463.01 0.9136 0.0356 1.7923 Hexokinase-2 P52789 HK2 4.80 3.2 2/2 102.38 4.640.59 2.210.88 0.4773 0.0353 1.8302 Lamin-B1 P14733 LMNB1 198.28 35.7 4/26 66.79 113.412.73 105.362.08 0.9290 0.0320 1.8425 Matrin-3 P43243 MATR3 40.77 11.8 1/6 94.62 14.270.48...”
- Designed Multifunctional Peptides for Intracellular Targets
Juretić, Antibiotics (Basel, Switzerland) 2022 - “...225 ]. The pentadecapeptide M(1)IASHLLAYFFTELN(15) is the VDAC-binding N-terminal domain of human HK2 (Uniprot entry P52789), acting as a surrogate peptide for HK2. HK2-VDAC association helps keep mitochondrial permeability transition pores in closed conformation when bound to the ATPsynthasome complex [ 226 ]. Mitochondria die together...”
- Integrated Brain Metabolomics and Network Pharmacology Analysis to Reveal the Improvement Effect of Bai Chan Ting on Parkinson's Disease.
Zhang, BioMed research international 2022 - “...P09467 Fructose-1 30 PLA2G1B P04054 Phospholipase A2 31 CYP3A4 P08684 Cytochrome P450 3A4 32 HK2 P52789 Hexokinase-2 33 HSPA5 P11021 Endoplasmic reticulum chaperone BiP 34 DAO P14920 D-amino-acid oxidase 35 GAA P10253 Lysosomal alpha-glucosidase 36 NOX4 Q9NPH5 NADPH oxidase 4 37 MAPK10 P53779 Mitogen-activated protein kinase...”
- Investigation of the active ingredients and pharmacological mechanisms of Porana sinensis Hemsl. Against rheumatoid arthritis using network pharmacology and experimental validation.
Hu, PloS one 2022 - “...0.48974359 2 44 P60568 IL2 0.00067511 0.48974359 2 45 P19367 HK1 0.00067511 0.48974359 2 46 P52789 HK2 0.00067511 0.48974359 2 47 P30542 ADORA1 0.01223823 0.49481865 6 48 P0DMS8 ADORA3 0.0151816 0.49739583 8 49 P10275 AR 0.00067511 0.48974359 2 50 P35869 AHR 0.00111812 0.49100257 3 51 P35354...”
- More
HXK4_ORYSJ / Q6Z398 Hexokinase-4, chloroplastic; Hexokinase II; EC 2.7.1.1 from Oryza sativa subsp. japonica (Rice) (see paper)
Q6Z398 hexokinase (EC 2.7.1.1) from Oryza sativa (see paper)
22% identity, 81% coverage
- function: Fructose and glucose phosphorylating enzyme.
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
Q8L5G8 Phosphotransferase from Zea mays
23% identity, 90% coverage
E9PB90 Hexokinase-2 from Homo sapiens
23% identity, 48% coverage
5hfuB / P52789 Crystal structure of human hexokinase 2 with cmpd 27, a 2-amido-6- benzenesulfonamide glucosamine (see paper)
23% identity, 47% coverage
- Ligand: ~{n}-[(2~{s},3~{r},4~{r},5~{s},6~{r})-6-[[(4-cyanophenyl)sulfonylamino]methyl]-2,4,5-tris(oxidanyl)oxan-3-yl]-3-phenyl-benzamide (5hfuB)
A9SV13 hexokinase (EC 2.7.1.1) from Physcomitrium patens (see paper)
20% identity, 85% coverage
NP_001079298 glucokinase (hexokinase 4) L homeolog from Xenopus laevis
25% identity, 58% coverage
F6Y855 Hexokinase-2 from Macaca mulatta
23% identity, 47% coverage
Q53QX9 Phosphotransferase (Fragment) from Homo sapiens
23% identity, 74% coverage
HXK1_SCHPO / Q09756 Hexokinase-1; EC 2.7.1.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
Q09756 hexokinase (EC 2.7.1.1) from Schizosaccharomyces pombe (see paper)
hxk1 / RF|NP_592948.1 hexokinase 1; EC 2.7.1.1; EC 2.7.1.2; EC 2.7.1.4; EC 2.7.1.7 from Schizosaccharomyces pombe (see 2 papers)
SPAC24H6.04 hexokinase 1 (PMID 8549830) from Schizosaccharomyces pombe
23% identity, 93% coverage
- function: Catalyzes the phosphorylation of hexose (six-carbon sugars) to hexose 6-phosphate (PubMed:8549830, PubMed:9790975). Phosphorylates D-fructose, D-mannose and, to a lower extent, D-glucose (PubMed:8549830, PubMed:9790975). Compared to hxk2, has low affinity for D-glucose (PubMed:8549830, PubMed:9790975).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
subunit: Monomer. - CharProtDB Source (per GeneDB): GeneDB_Spombe
- Pro-aging effects of glucose signaling through a G protein-coupled glucose receptor in fission yeast
Roux, PLoS genetics 2009 - “...pombe genome project nomenclature git3 + (SPCC1753.02c); gpa2 + / git8 + (SPAC23H3.13c); hxk1 + (SPAC24H6.04); hxk2 + (SPAC4F8.07c); fbp1 + (SPBC1198.14c); sod1 + (SPAC821.10c); sod2 + (SPAC1486.01); gpx1 + (SPBC32F12.03c); top1 + (SPBC1703.14c); unnamed a chloride channel (SPBC1703.14c) Supporting Information Figure S1 Growth curves of...”
LOC542510 uncharacterized protein LOC542510 from Zea mays
K7VX77 Phosphotransferase from Zea mays
23% identity, 87% coverage
XP_004533719 hexokinase-2 from Ceratitis capitata
23% identity, 84% coverage
- Genomic signatures of globally enhanced gene duplicate accumulation in the megadiverse higher Diptera fueling intralocus sexual conflict resolution
Bao, PeerJ 2020 - “...XP_004518508, XP_004519116, XP_004519322, XP_004520566, XP_004521010, XP_004523446, XP_004523968, XP_004524766, XP_004526337, XP_004529383, XP_004529412, XP_004530158, XP_004531038, XP_004531266, XP_004533385, XP_004533719, XP_004535304, XP_004536134, XP_004536932, XP_004537692, XP_005187487, XP_005190203, XP_005190609, XP_006557645, XP_006558427, XP_006560286, XP_006563718, XP_006564634, XP_006564913, XP_006565977, XP_008191172, XP_008194238, XP_008199565, XP_008201416, XP_008201713, XP_019891905, XP_020713613, XP_021693932, XP_310951, XP_311387, XP_316164, XP_391836, XP_391836, XP_392478, XP_392899.2, XP_395280,...”
HXK_SCHMA / Q26609 Hexokinase; EC 2.7.1.1 from Schistosoma mansoni (Blood fluke) (see paper)
Smp_043030 hexokinase from Schistosoma mansoni
23% identity, 93% coverage
1bdgA / Q26609 Hexokinase from schistosoma mansoni complexed with glucose (see paper)
23% identity, 93% coverage
- Ligand: alpha-d-glucopyranose (1bdgA)
A0A220NLA7 hexokinase (EC 2.7.1.1) from Macrobrachium nipponense (see paper)
23% identity, 87% coverage
TRIREDRAFT_73665 hexokinase from Trichoderma reesei QM6a
23% identity, 98% coverage
XP_021723531 hexokinase-1-like from Chenopodium quinoa
24% identity, 86% coverage
- Salinity Effects on Guard Cell Proteome in Chenopodium quinoa
Rasouli, International journal of molecular sciences 2021 - “...EULS3 2.0 * XP_021754221 MMK2-like 1.6 * XP_021759066 GDPDL3-like 1.5 * XP_021738483 OST1 1.3 * XP_021723531 hexokinase-1-like 0.9 * XP_021715572 clathrin heavy chain 1-like 0.8 * XP_021723433 plasma membrane ATPase 4-like 0.5 * XP_021761237 calcium sensing receptor, chloroplastic-like 0.5 * XP_021734330 protein flowering locus t-like 0.3...”
PAAG_01015 hexokinase from Paracoccidioides lutzii Pb01
23% identity, 84% coverage
- Interaction of Isocitrate Lyase with Proteins Involved in the Energetic Metabolism in Paracoccidioides lutzii
Freitas, Journal of fungi (Basel, Switzerland) 2020 - “...protein # 0.649 PAAG_07729 isocitrate dehydrogenase * 0.638 PAAG_11349 peroxisomal biogenesis factor 6 & 0.626 PAAG_01015 Hexokinase & 0.624 PAAG_08449 malate dehydrogenase * 0.621 PAAG_0058 fumarate hydratase * 0.607 PAAG_04856 acetyl-CoA hydrolase 0.605 PAAG_08057 cytochrome c oxidase * 0.602 * Proteins specific from yeast protein extract;...”
- Transcriptional and proteomic responses to carbon starvation in Paracoccidioides
Lima, PLoS neglected tropical diseases 2014 - “...* cobalamin (vitamin B12) biosynthesis PAAG_01519 inositol monophosphatase * secondary metabolism ENERGY Glycolysis and gluconeogenesis PAAG_01015 hexokinase * glycolysis Electron transport and membrane-associated energy conservation PAAG_07285 vacuolar ATP synthase catalytic subunit A * ATP hydrolysis coupled proton transport PAAG_06288 vacuolar ATP synthase subunit B * ATP...”
A9TLJ2 hexokinase (EC 2.7.1.1) from Physcomitrium patens (see paper)
22% identity, 85% coverage
3b8aX / P04806 Crystal structure of yeast hexokinase pi in complex with glucose (see paper)
24% identity, 88% coverage
- Ligand: beta-d-glucopyranose (3b8aX)
Q9SEK2 Hexokinase-1 from Nicotiana tabacum
23% identity, 86% coverage
- Low temperature modifies seedling leaf anatomy and gene expression in Hypericum perforatum.
Su, Frontiers in plant science 2022 - “...Dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex, chloroplastic -0.21 ENO1 P42896 Enolase 0.69 HXK1 Q9SEK2 Hexokinase-1 0.37 HXK2 P93834 Hexokinase-2 0.29 PFP-ALPHA Q41140 Pyrophosphatefructose 6-phosphate 1-phosphotransferase subunit alpha 0.26 PFP-BETA Q41141 Pyrophosphatefructose 6-phosphate 1-phosphotransferase subunit beta 0.33 Fatty acid metabolism (11) CUT1 Q9XF43 3-ketoacyl-CoA synthase...”
- iTRAQ-Based Proteomic Analysis Reveals Potential Regulation Networks of IBA-Induced Adventitious Root Formation in Apple.
Lei, International journal of molecular sciences 2018 - “...9 Q9SCV3 77.39 16.6 5 1.71 MDP0000237069 -galactosidase Q9FXT4 25.52 8.4 2 1.71 MDP0000823956 Hexokinase Q9SEK2 78.16 13.7 3 1.65 MDP0000294262 6-phosphofructokinase Q41141 82.18 8.1 1 0.4 MDP0000254412 6-phosphofructokinase Q8VYN6 79.88 5.5 1 1.55 MDP0000835914 Glyceraldehyde-3-phosphate dehydrogenase P12859 88.47 16.2 5 1.62 MDP0000527995 Glyceraldehyde-3-phosphate dehydrogenase P12858...”
LOC103843922 hexokinase-like 1 protein from Brassica rapa
22% identity, 83% coverage
LOC105671942 hexokinase type 2 from Linepithema humile
24% identity, 83% coverage
- Social environment affects the transcriptomic response to bacteria in ant queens
Viljakainen, Ecology and evolution 2018 - “...beta E chain (LOC105675407) 1.10 3.55E04 5.92E02 LOC105667297 Protein LTV1 homolog (LOC105667297) 1.08 4.42E04 7.27E02 LOC105671942 Hexokinase2like (LOC105671942) 1.08 8.18E05 2.05E02 LOC105677187 Serine protease easterlike (LOC105677187) 1.05 1.09E04 2.33E02 LOC105677615 Alphatocopherol transfer proteinlike (LOC105677615) 1.38 3.07E05 9.22E03 LOC105675717 Defensing2 (LOC105675717) 1.39 4.68E04 7.52E02 LOC105679818 Cytosolic 10formyltetrahydrofolate...”
- “...4.27E04 3.08E02 LOC105676184 Troponin C 1.09 2.32E04 1.95E02 LOC105678432 AcetylCoA carboxylase (LOC105678432) 1.11 9.56E04 6.02E02 LOC105671942 Hexokinase2like (LOC105671942) 1.12 9.69E04 6.03E02 LOC105672608 Facilitated trehalose transporter Tret1like (LOC105672608) 1.14 7.47E04 4.92E02 LOC105678761 Serine protease inhibitor 3/4like (LOC105678761) 1.17 4.04E04 2.98E02 LOC105675773 Peptidoglycanrecognition protein SC2like (LOC105675773) 1.20 9.78E06...”
B8K2W7 hexokinase (EC 2.7.1.1) from Helianthus annuus (see paper)
24% identity, 90% coverage
Q3UE51 hexokinase (Fragment) from Mus musculus
24% identity, 45% coverage
HXK2_RAT / P27881 Hexokinase-2; Hexokinase type II; HK II; Hexokinase-B; EC 2.7.1.1 from Rattus norvegicus (Rat) (see paper)
P27881 hexokinase (EC 2.7.1.1) from Rattus norvegicus (see 2 papers)
NP_036867 hexokinase-2 from Rattus norvegicus
23% identity, 46% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (PubMed:5871820). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:5871820). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (By similarity).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
subunit: Monomer (By similarity). Interacts with TIGAR; the interaction increases hexokinase activity in a hypoxia- and HIF1A-dependent manner (By similarity). - ITRAQ Based Proteomics Reveals the Potential Mechanism of Placental Injury Induced by Prenatal Stress.
Li, International journal of molecular sciences 2024 - “...Isoform 2 of Hydroxyacylglutathione hydrolase, mitochondrial (Hagh) D4A3K5 Histone H1.1 (Hist1h1a) D3ZBN0 Histone H1.5 (Hist1h1b) P27881 Hexokinase-2 (Hk2) Q6P747 Heterochromatin protein 1-binding protein 3 (Hp1bp3) Q6AYS8 Estradiol 17-beta-dehydrogenase 11 (Hsd17b11) Q5I0N4 Estradiol 17-beta-dehydrogenase 2 (Hsd17b2) P0DMW0 Heat shock 70 kDa protein 1A (Hspa1a) F1LTJ5 Uncharacterized protein...”
- Investigation on Intestinal Proteins and Drug Metabolizing Enzymes in Simulated Microgravity Rats by a Proteomics Method.
Liu, Molecules (Basel, Switzerland) 2020 - “...dehydrogenase family 1 member A3 (ALDH1A3) 3.37 0.0121 P07379 Cytosolic phosphoenolpyruvate carboxykinase (PCK1) 3.08 0.0122 P27881 Hexokinase-2 (HK2) 2.58 0.0214 P05708 Hexokinase-1 (HK1) 2.40 0.0031 P11884 mitochondrial Aldehyde dehydrogenase (ALDH2) 2.33 0.0005 P30839 Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 2.17 0.0005 P30835 ATP-dependent 6-phosphofructokinase, liver...”
- Inflammation and apoptosis accelerate progression to irreversible atrophy in denervated intrinsic muscles of the hand compared with biceps: proteomic analysis of a rat model of obstetric brachial plexus palsy.
Yu, Neural regeneration research 2020 - “...* , P12369 * , G3V8R5 * , Q9Z2S9 * , P19357, M0RD75, D3ZM60, P13286, P27881, P62142, F1LLZ7, A2RRU1, P97531, G3V8V3, Q4G050, A0A0G2K9C8, Q6P727, Q9Z1N1 0.002 Pyruvate metabolism 17 A0A0G2K5G8 * , P52873 * , Q6P7Q4, P11980, P04636, A0A0G2K4C6, P35745, F7FKI5, P17764, O88989, P42123, P08461, P14408,...”
- “...B0BN46 < 0.001 HIF-1 pathway 14 Q3HSE5 * , Q9ET45 * , P15429, M0RD75, A0A0G2K7M1, P27881, P07323, M0R660, Q4G050, F7FKI5, P49432, E9PTN6, P11275, G3V679, E9PTV9 0.006 Calcium pathway 22 F1LV12 * , F1LMY4, P63329, Q304F3, Q64578, P13286, A0A0G2K5J1, F1LPJ2, Q4PP99, B3XZL8, F1LLZ7, A0A0H2UHV6, G3V731, P81155, A0A0G2K9C8,...”
- Phenobarbital induces alterations in the proteome of hepatocytes and mesenchymal cells of rat livers.
Klepeisz, PloS one 2013 - “...shock-related 70 kDa protein 2 (Heat shock protein 70.2) multicellular organismal development, response to stress P27881 Hexokinase-2 (Hexokinase type II) (HK II) cellular glucose homeostasis, apoptotic mitochondrial changes Q920F3 KH domain-containing, RNA-binding, signal transduction-associated protein 2 (SLM-1) regulation of transcription, DNA-dependent O08816 Neural Wiskott-Aldrich syndrome protein...”
- Extent of mitochondrial hexokinase II dissociation during ischemia correlates with mitochondrial cytochrome c release, reactive oxygen species production, and infarct size on reperfusion.
Pasdois, Journal of the American Heart Association 2012 - “...present in the center of the hydrophobic Nterminal peptide (MIASHMIACL in rat from accession number P27881) required for mitochondrial binding. 15 It is likely that this histidine needs to be uncharged to insert into the OMM, and this would be its predominant ionization state at physiological...”
- Chronic morphine alters the presynaptic protein profile: identification of novel molecular targets using proteomics and network analysis.
Abul-Husn, PloS one 2011 - “...Rat Brain Hexokinase Type I Complex With Glucose And Inhibitor Glucose-6-Phosphate P05708 HK2 Hexokinase 2 P27881 VDAC2 Voltage-dependent anion-selective channel protein 2 P81155 VDAC3 Voltage-dependent anion-selective channel protein 3 Q9R1Z0 Upregulated (7) Mitochondrial-related ATP5C1 ATP synthase gamma chain, mitochondrial P35435 ATP5B ATP synthase subunit beta, mitochondrial...”
- A proteomic survey of rat cerebral cortical synaptosomes
Witzmann, Proteomics 2005 - “...Q9WTT6 O70351 Q63147 P35429 P55063 P14659 P34058 P05708 P27881 P41565 P97607 P47858 P30835 P47860 P39069 P11275 P08413 P15791 P11730 P07335 P09605 P25809 Q9Z0J8...”
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...(NP_036866), Mus musculus (NP_001139572), and Bos taurus (AAA51661). Hexokinases 2: H. sapiens (AAH21116), R. norvegicus (NP_036867), M. musculus (AAH54472), D. rerio (AAH45496), and S. pombe HK2 (CAA63488). Hexokinase 3: M. musculus (NP_001028417), R. norvegicus (NP_071515), H. sapiens (NP_002106), Macaca mulatta (XP_001086179), and Nicotiana sylvestris (AAT77513). Invertebrate...”
C5YUA1 Phosphotransferase from Sorghum bicolor
Sb09g005840 No description from Sorghum bicolor
22% identity, 80% coverage
NP_524674 hexokinase C from Drosophila melanogaster
Q7JYW9 Phosphotransferase from Drosophila melanogaster
25% identity, 89% coverage
NP_001233957 hexokinase from Solanum lycopersicum
23% identity, 86% coverage
LOC110729879 hexokinase-2-like from Chenopodium quinoa
25% identity, 87% coverage
- Molecular mechanisms regulating glucose metabolism in quinoa (Chenopodium quinoa Willd.) seeds under drought stress
Wang, BMC plant biology 2024 - “...with CqINV (LOC110717458), CqFK4 (LOC110718125), CqCELB (LOC29490), Cqbg1x (LOC110695967, LOC110685748), CqSUS (LOC110689796, LOC110727927), and CqHK2 (LOC110729879), respectively (PCC0.6). Fructose-6-phosphate, D-glucose-6-phosphate, and D-glucose-1-phosphate were highly positively correlated with CqINV (LOC110717458), and PCCs were 0.98, 0.91, 0.93, respectively. UDP-glucose and D-glucose-1-phosphate were highly positively correlated with CqSUS (LOC110689796),...”
LOC107876107 hexokinase-2 from Capsicum annuum
23% identity, 90% coverage
- Fine mapping of a thrips resistance QTL in Capsicum and the role of diterpene glycosides in the underlying mechanism
van, TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 2021 - “...Profilin-2 24.5324.54 Mbp LOC107874799 Putative protein TPRXL 24.5424.55 Mbp LOC107876106 Protein IQ-DOMAIN 31-like 24.5624.56 Mbp LOC107876107 Hexokinase-2 24.5624.57 Mbp LOC107876108 Nuclear poly(A) polymerase 4-like 24.7324.74 Mbp LOC107876109 Organic cation/carnitine transporter 7-like 24.8124.82 Mbp LOC107876110 Organic cation/carnitine transporter 7-like 24.8324.84 Mbp LOC107874800 Uncharacterized LOC107874800 24.9524.95 Mbp LOC107874801...”
Q6X271 hexokinase (EC 2.7.1.1) from Physcomitrium patens (see paper)
22% identity, 83% coverage
XP_002459072 hexokinase-3 from Sorghum bicolor
25% identity, 61% coverage
- Hexose kinases and their role in sugar-sensing and plant development
Granot, Frontiers in plant science 2013 - “...( 2008 ), Cheng et al. ( 2011 ) Sorghum ( Sorghum bicolor ) SbHXK3 XP_002459072 Type B/M No Glc sensing role Karve et al. ( 2010 ) SbHXK8 XP_002455027 C Karve et al. ( 2010 ) Wheat (Triticum aestivum) HXK AY974231 ND Controls triose-phosphate/phosphate translocation...”
CTRG_00414 hexokinase from Candida tropicalis MYA-3404
24% identity, 97% coverage
- Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol
Wang, Frontiers in microbiology 2020 - “...in Figure 8A , during the conversion of glucose to chitin, the expression levels of CTRG_00414, CTRG_00601, CTRG_01436, CTRG_03651, CTRG_03585, CTRG_05721, and CTRG_05949 were markedly increased. In addition, most of the genes in the chitin degradation pathway (chitin to chitobiose or N-Acetyl-D-glucosamine), including CTRG_05456 and CTRG_05827...”
VDAG_01913 glucokinase from Verticillium dahliae VdLs.17
23% identity, 79% coverage
M0ZRE9 Phosphotransferase from Solanum tuberosum
23% identity, 86% coverage
O74996 hexokinase (EC 2.7.1.1) from Yarrowia lipolytica (see paper)
hxk1 / CAA09674.1 hexokinase from Yarrowia lipolytica (see paper)
hxk1 / CAA09675.1 Hexokinase from Yarrowia lipolytica (see paper)
25% identity, 90% coverage
O54892 Hexokinase-2 from Rattus norvegicus
23% identity, 46% coverage
B7QB25 Phosphotransferase (Fragment) from Ixodes scapularis
22% identity, 90% coverage
NGK1_YEAST / Q06204 N-acetylglucosamine kinase; EC 2.7.1.59 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
NP_013551 hexokinase from Saccharomyces cerevisiae S288C
YLR446W Putative protein of unknown function with similarity to hexokinases; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene from Saccharomyces cerevisiae
23% identity, 90% coverage
- function: N-acetylglucosamine (GlcNAc) kinase that transfers a phosphate onto GlcNAc to generate GlcNAc-6-phosphate, which can be a precursor for glycan synthesis (PubMed:36216916). Can utilize a broad range of NTPs, namely ATP, CTP, GTP, ITP, and UTP, as phosphoryl donors (PubMed:36216916). Shows only low glucose phosphorylation activity (PubMed:36216916). NGK1 may play a role under a certain condition as an alternative supply pathway GlcNAc-6-P as a precursor of UDP-GlcNAc (Probable).
catalytic activity: N-acetyl-D-glucosamine + ATP = N-acetyl-D-glucosamine 6- phosphate + ADP + H(+) (RHEA:17417)
catalytic activity: N-acetyl-D-glucosamine + GTP = N-acetyl-D-glucosamine 6- phosphate + GDP + H(+) (RHEA:74647)
catalytic activity: N-acetyl-D-glucosamine + CTP = N-acetyl-D-glucosamine 6- phosphate + CDP + H(+) (RHEA:74651)
catalytic activity: ITP + N-acetyl-D-glucosamine = IDP + N-acetyl-D-glucosamine 6- phosphate + H(+) (RHEA:74655)
catalytic activity: N-acetyl-D-glucosamine + UTP = N-acetyl-D-glucosamine 6- phosphate + UDP + H(+) (RHEA:74659)
cofactor: Mg(2+) Mn(2+) Co(2+) - Ngk1 kinase-mediated N-acetylglucosamine metabolism promotes UDP-GlcNAc biosynthesis in Saccharomyces cerevisiae.
Nishikawa, FEBS letters 2024 (PubMed)- GeneRIF: Ngk1 kinase-mediated N-acetylglucosamine metabolism promotes UDP-GlcNAc biosynthesis in Saccharomyces cerevisiae.
- Rap1 overexpression boosts triterpenoid saponin production in yeast by enhancing precursor supply and heterologous gene expression
Byun, Microbial cell factories 2025 (no snippet) - Genomic, transcriptomic, and metabolic characterization of 2-Phenylethanol-resistant Saccharomyces cerevisiae obtained by evolutionary engineering
Holyavkin, Frontiers in microbiology 2023 - “...NQM1 CRH1 XKS1 SOL4 SCW4 BGL2 IMA1 MAL12 YHR210C PKP1 SGA1 YIR007W IMA5 MDH1 CTS1 YLR446W PGM2 PGM3 CAT8 SOL1 MDH2 GAL4 ATH1 GPH1 GDB1 36 94 Cellular response to oxidative stress [GO:0034599] PRX1 UGA2 GRX7 ZTA1 YBP1 GRX1 GRX6 YDL124W YPR1 TSA2 GRX2 HSP12 MTL1...”
- “...GSY2 PGM2 GAC1 GDB1 8 12 Glycolysis [GO:0006096] GLK1 GPM2 EMI2 HXK1 ENO1 TDH1 FBA1 YLR446W ERR3 PYK2 ERR1 ERR2 12 28 Oxidationreduction process [GO:0055114] COX1 COX2 COX3 BDH1 BDH2 PRX1 UGA2 ETR1 ZTA1 ARA1 HBN1 GPD1 NDE2 YDL124W GDH2 PAM1 YPR1 TSA2 SER3 MET13 ARI1...”
- Saccharomyces cerevisiae responds similarly to co-culture or to a fraction enriched in Metschnikowia pulcherrima extracellular vesicles
Mejias-Ortiz, Microbial biotechnology 2023 - “...overexpressed while only two of them, that are not an integral part of the pathway (YLR446W and YMR110C), appear downregulated. Although this pathway is labelled as Glycolysis/Gluconeogenesis the results indicate that mostly the glycolytic pathway is upregulated. Certainly, MDH2 (YOL126C) coding for the enzyme catalysing the...”
- Identification and biochemical characterization of a novel N-acetylglucosamine kinase in Saccharomyces cerevisiae
Umekawa, Scientific reports 2022 - “...on the presence of two other hexokinase-like genes (but whose functions were unknown): EMI2 and YLR446W . In our previous work, the Emi2 protein was identified as a fourth new hexokinase of S. cerevisiae in addition to the previously known ones: Hxk1, Hxk2, and Glk1 13...”
- “...whether it is an additional new hexokinase in this model organism. Unexpectedly, we found that YLR446W encodes a novel GlcNAc kinase that shows low sequence similarity to known GlcNAc kinases from other organisms. Results The YLR446W protein is a kinase with low glucose phosphorylation activity The...”
- Genome-scale modeling drives 70-fold improvement of intracellular heme production in Saccharomyces cerevisiae
Ishchuk, Proceedings of the National Academy of Sciences of the United States of America 2022 - “...production ( SI Appendix, Fig. S3 ). Additional deletion of FAA4 , FDH1 , and YLR446w resulted only in a small improvement of heme production ( SI Appendix, Figs. S4 and S5 ). The deletion of GCV2 improved heme production in combination with only some genes...”
- Metabolic flux sampling predicts strain-dependent differences related to aroma production among commercial wine yeasts
Scott, Microbial cell factories 2021 - “...Tyrosine metabolism, Biosynthesis of secondary metabolites (Ehrlich pathway), Fatty acid degradation GLK1 HXK1 HXK2 EMI2 YLR446W YCL040W YFR053C YGL253W YDR516C Hexokinase (D-glucose:ATP) r_0534 Glycolysis, Gluconeogenesis, Fructose and mannose metabolism, Galactose metabolism, Amino sugar and nucleotide sugar metabolism, Carbon metabolism, Biosynthesis of secondary metabolites AAT2 YLR027C Aspartate...”
- A novel yeast hybrid modeling framework integrating Boolean and enzyme-constrained networks enables exploration of the interplay between signaling and metabolism
Österberg, PLoS computational biology 2021 - “...enzyme-constrained and hybrid model. In both conditions the FCCs obtained for hexokinases by the ecModel (YLR446W for respiration and HXK1 in fermentation) showed a value equal to 1, the highest value in their respective distributions, indicating that the overall glucose uptake rate is mostly governed by...”
- Transcriptional Comparison Investigating the Influence of the Addition of Unsaturated Fatty Acids on Aroma Compounds During Alcoholic Fermentation
Yan, Frontiers in microbiology 2019 - “...TIP1 Major cell wall mannoprotein with possible lipase activity 0.46 YLR094C GIS3 GIg1-2 suppressor 0.50 YLR446W Putative hexokinase 0.43 YLL013C PUF3 Protein of the mitochondrial outer surface 0.50 YGL169W SUA5 Protein involved in threonylcarbamoyl adenosine biosynthesis 0.49 YPL119C DBP1 Putative ATP-dependent RNA helicase of DEAD-box protein...”
- More
LOC18035909 hexokinase-1 from Citrus x clementina
22% identity, 91% coverage
- Transcriptome analysis of Harumi tangor fruits: Insights into interstock-mediated fruit quality
Liao, Frontiers in plant science 2022 - “...( PKp3 , LOC18043314), inactive beta-amylase ( BMY3 , LOC18032416), and hexokinase ( HXK1 , LOC18035909). In contrast, the expression levels of several other genes were >2-fold higher in the HPP1 group, including genes encoding endoglucanases (LOC18048123, LOC18052685, LOC18033025), PG (LOC18031830, LOC18047316), BGLU13 (LOC18044658), XYL4 (LOC18041989),...”
- “...IDH-III (LOC18037111), fructokinase gene FRK4 (LOC18046804), alkaline/neutral invertase gene CINV2 (CICLE_v10003734mg, LOC18042730), hexokinase gene HXK1 (LOC18035909), and sucrase/ferredoxin-like gene (LOC18051223) were downregulated in the HPP group at 45, 90, and 180 DAF and upregulated in the HPP group at 135 DAF. The sucrose-phosphate synthase gene SPS3F...”
- Combined Transcriptome and Metabolome Analyses Reveal Candidate Genes Involved in Tangor (Citrus reticulata × Citrus sinensis) Fruit Development and Quality Formation
Bi, International journal of molecular sciences 2022 - “...b). Among the DEGs associated with starch and sucrose metabolism, the sucrose degradation-related gene HXK1 (LOC18035909) and the starch degradation-related genes PTPKIS1 (LOC18043211) and AMY1 (LOC18043125) were up-regulated throughout development and ripening, and the starch degradation-related gene BMY5 (LOC18053108) was down-regulated throughout fruit development. Three of...”
- Comparative transcriptomics of wild and commercial Citrus during early ripening reveals how domestication shaped fruit gene expression
Borredá, BMC plant biology 2022 - “...the domestication process. For instance, the citron allele of one hexokinase gene ( HK , LOC18035909) was preferentially expressed in lemon pulp, while in sweet orange the pummelo allele was the one overexpressed. The pummelo allele of a GABA transporter (LOC18035651), which might play a role...”
PAS_chr4_0624 Non-essential protein of unknown function required for transcriptional induction from Komagataella phaffii GS115
25% identity, 86% coverage
- Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
Li, Genes 2019 - “...comparison of gene expression between PI1 and PI2, only the log 2 (fold change) of PAS_chr4_0624 and PAS_chr1-4_0561 (EC2.7.1.1) was over 1, and the expression of other genes involved in the central carbon metabolic pathway did not show significant variation based on our screening criteria (...”
- Translational arrest due to cytoplasmic redox stress delays adaptation to growth on methanol and heterologous protein expression in a typical fed-batch culture of Pichia pastoris
Edwards-Jones, PloS one 2015 - “...in hexose metabolism were not expected. Therefore, transient induction of hexokinase (PAS_chr1-4_0447, PAS_chr3_1192, PAS_chr1-4_0561 and PAS_chr4_0624), phosphoglucomutase (PAS_chr 1-4_0264), glucose-6-phosphate dehydrogenase (PAS_chr 2-1_0308) suggest that during adaptation to growth on methanol, metabolic reserves of glycogen, trehalose and cell wall ,D glucans (see below) were being degraded....”
GRMZM2G051806 hexokinase-1 from Zea mays
21% identity, 82% coverage
- Genetic Dissection of Phosphorus Use Efficiency in a Maize Association Population under Two P Levels in the Field
Li, International journal of molecular sciences 2021 - “...metabolomic data of extreme inbred lines; through this, three potential candidate genes GRMZM2G039588, GRMZM5G841893, and GRMZM2G051806 were found, and several were confirmed in RILs. Similarly, Wang et al. [ 31 ] used 13 root-related phenotypes at different P levels at the seedling stage to perform GWAS...”
- The utility of metabolomics as a tool to inform maize biology
Medeiros, Plant communications 2021 - “...GWAS Lpez-Malvar etal. (2019) Mechanisms of phosphorus deficiency in leaves and roots LC-MS and GC-MS GRMZM2G051806 GRMZM2G025854 GRMZM2G039588 GRMZM2G050570 GRMZM5G841893 GWAS Luo etal. (2019) Tocopherol content in leaves and kernels ELISA and LC porb2 QTL and association mapping Zhan etal. (2019) Antibiotic biosynthesis (zealexin) GC-MS and...”
- “...detector; GRMZM2G008714, pyruvate kinase; GRMZM2G025854, phosphoglucomutase; GRMZM2G039588, glucose-6-phosphate 1-epimerase; GRMZM2G045171, sucrose synthase; GRMZM2G050570, threonine synthase; GRMZM2G051806, hexokinase; GRMZM2G082780, phosphoenolpyruvate carboxylase 4; GRMZM2G088235, urease protein; GRMZM2G110929, GLABRA2 expression modulator; GRMZM2G180625, glyceraldehyde-3-phosphate dehydrogenase; GRMZM2G437912, paralog gene encoding a prephenate dehydratase; GRMZM5G841893, FAD-dependent urate hydroxylase; GRMZM5G852704, ethylene-responsive transcription factor...”
- Metabolite profiling and genome-wide association studies reveal response mechanisms of phosphorus deficiency in maize seedling
Luo, The Plant journal : for cell and molecular biology 2019 - “...(lowPi/control) ratio Leaf of inbred lineB73 under Pi deficiency/log 2 (lowPi/control) ratio Glucose glucose6phosphate Hexokinase GRMZM2G051806 Primary root fresh weightCK PZE106074800FWPR 6 129981085 1.07E5 0.06156 2.184 3.135 3.318 1.402 Glucose glucose6phosphate Phosphoglucomutase GRMZM2G025854 Total root fresh weightINDEX PZE103171255 3 218258052 1.65E6 0.07265 1.317 3.601 0.488 Glucose...”
- “...upregulated in leaves and downregulated in roots (Figure 8 a and b). GRMZM2G050570, GRMZM2G039588 and GRMZM2G051806 all showed a decreasing or balanced tendency after 14days of Pi treatment, except GRMZM2G039588 in line 9782 roots at 14days (Figure 8 ch). GRMZM5G841893 was barely expressed in leaves but...”
A0A1D6NCX2 hexokinase (EC 2.7.1.1) from Zea mays (see paper)
24% identity, 77% coverage
Q59RR7 Phosphotransferase from Candida albicans (strain SC5314 / ATCC MYA-2876)
26% identity, 70% coverage
Q96VP5 hexokinase (EC 2.7.1.1) from Ogataea angusta (see paper)
22% identity, 86% coverage
Hxk2 / Q6Q8A5 hexokinase (EC 2.7.1.1; EC 2.7.1.4; EC 2.7.1.7) from Nicotiana tabacum (see paper)
HXK2_TOBAC / Q6Q8A5 Hexokinase-2, chloroplastic; NtHxK2; EC 2.7.1.1 from Nicotiana tabacum (Common tobacco) (see paper)
Q6Q8A5 hexokinase (EC 2.7.1.1) from Nicotiana tabacum (see paper)
23% identity, 88% coverage
- function: Fructose and glucose phosphorylating enzyme.
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
HXK_BRUMA / A0A0K0JFP3 Hexokinase; BmHK; EC 2.7.1.1 from Brugia malayi (Filarial nematode worm) (see paper)
24% identity, 67% coverage
- function: Active against glucose, fructose, mannose, maltose and galactose.
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
HXK2_SOLTU / Q9SQ76 Hexokinase-2; StHK2; EC 2.7.1.1 from Solanum tuberosum (Potato) (see paper)
Q9SQ76 hexokinase (EC 2.7.1.1) from Solanum tuberosum (see paper)
23% identity, 86% coverage
- function: Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from plastids to cytosol. Seems neither to be involved in cell sugar sensing nor in carbohydrate metabolism in tuber.
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
XP_015157917 hexokinase-4 isoform X1 from Gallus gallus
22% identity, 59% coverage
HXK3_ORYSJ / Q2KNB4 Hexokinase-3; Hexokinase-8; Protein SHORT AND NARROW GRAIN 1; EC 2.7.1.1 from Oryza sativa subsp. japonica (Rice) (see 2 papers)
23% identity, 90% coverage
- function: Fructose and glucose phosphorylating enzyme (PubMed:16552590). Involved in the regulation of cell expansion in spikelet hulls, grain size, and gibberellin biosynthesis and homeostasis (PubMed:35941236).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
disruption phenotype: Decreased grain length, grain width, and grain filling (PubMed:35941236). Delayed seed germination and retarded seedling growth (PubMed:35941236).
HCAG_03191 glucokinase from Histoplasma mississippiense (nom. inval.)
23% identity, 82% coverage
XP_046759294 hexokinase-2 isoform X1 from Gallus gallus
24% identity, 27% coverage
A9SJG4 hexokinase (EC 2.7.1.1) from Physcomitrium patens (see paper)
21% identity, 85% coverage
2nztB / P52789 Crystal structure of human hexokinase ii (see paper)
23% identity, 49% coverage
- Ligands: alpha-d-glucopyranose; 6-o-phosphono-beta-d-glucopyranose (2nztB)
HCBG_05633 glucokinase glkA from Histoplasma capsulatum G186AR
22% identity, 85% coverage
- Insights Into Histoplasma capsulatum Behavior on Zinc Deprivation
Assunção, Frontiers in cellular and infection microbiology 2020 - “...Deprivation Decreases Fermentation in H. capsulatum The proteomic analysis showed that enzymes such as hexokinase (HCBG_05633), enolase (HCBG_00056), pyruvate carboxylase (HCBG_00107), and alcohol dehydrogenase (HCBG_05406) were repressed in zinc deprivation. Such results indicate that anaerobic metabolism is suppressed at low zinc levels. Thus, to confirm these...”
- “...also observed ( Supplementary Tables 02 05 ). Specifically, in glycolysis, two regulatory enzymes: hexokinase (HCBG_05633) and phosphofrutokinase-1 (HCBG_08430), were repressed, indicating that the glycolytic pathway was oxidizing glucose at a reduced rate. These results corroborate with Medina and Nicholas (1957) who showed a 70% reduction...”
LOC101216058 hexokinase-2, chloroplastic from Cucumis sativus
21% identity, 89% coverage
HXK5_ORYSJ / Q5W676 Hexokinase-5; Hexokinase I; EC 2.7.1.1 from Oryza sativa subsp. japonica (Rice) (see 3 papers)
Q5W676 hexokinase (EC 2.7.1.1) from Oryza sativa (see paper)
NP_001407676 hexokinase-5 from Oryza sativa Japonica Group
22% identity, 94% coverage
- function: Fructose and glucose phosphorylating enzyme (PubMed:16552590). Functions as a glucose sensor for plant growth and photosynthesis (PubMed:19010999). Is essential for pollen development, germination, and tube growth (PubMed:31671177). Its activity is necessary for the starch utilization pathway during pollen germination and tube growth, as well as for starch biosynthesis during pollen maturation (PubMed:31671177).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
disruption phenotype: Male sterility of pollen grains due to impaired synthesis and utilization of starch in pollen grains. - Deficiency of rice hexokinase HXK5 impairs synthesis and utilization of starch in pollen grains and causes male sterility.
Lee, Journal of experimental botany 2020 (PubMed)- GeneRIF: Deficiency of rice hexokinase HXK5 impairs synthesis and utilization of starch in pollen grains and causes male sterility.
- Physiological and Proteome Analysis of the Effects of Chitosan Oligosaccharides on Salt Tolerance of Rice Seedlings
Qian, International journal of molecular sciences 2024 - “...1.8.1.4 K00382 B9FMK1 1.8.1.4 K00382 A0A0D9V0H3 Root 5.4.2.2 K01835 A0A0E0D5L3 2.7.1.1 K00844 A0A0D9YES9 2.7.1.1 K00844 Q5W676 5.3.1.9 K01810 A0A0E0M263 2.7.1.1 K00844 A0A0D3ETH5 5.1.3.3 K01785 A0A0E0HWM4 3.1.3.11 K03841 A0A0D3G850 2.7.1.11 K24182 A0A0D9Y4F5 2.7.1.11 K24182 J3N2I3 4.2.1.11 K01689 I1QSV0 2.7.1.11 K24182 A0A0D9Y4F5 4.2.1.11 K01689 B8AK24 2.7.1.11 K24182 A0A0P0WBF4...”
- Quantitative Proteomic Analysis Provides Insights into Rice Defense Mechanisms against Magnaporthe oryzae
Lin, International journal of molecular sciences 2018 - “...1.53 0.87 1.50 S4TZU3 Os02g0621700 magnesium ion binding 1.23 1.49 0.79 1.31 response to chemical Q5W676 HXK5 fructose and glucose phosphorylating enzyme 1.06 1.27 0.79 1.43 Q2QYK6 chalcone isomerase chalcone isomerase activity 1.15 1.4 0.94 1.59 Q852M0 GDH1 glutamate dehydrogenase activity 1.37 1.49 0.83 1.45 Q8S718...”
4qs7A / Q42525 Arabidopsis hexokinase 1 (athxk1) structure in glucose-bound form (see paper)
23% identity, 93% coverage
- Ligand: beta-d-glucopyranose (4qs7A)
NCU00575 glucokinase from Neurospora crassa OR74A
22% identity, 55% coverage
- Deletion of the col-26 Transcription Factor Gene and a Point Mutation in the exo-1 F-Box Protein Gene Confer Sorbose Resistance in Neurospora crassa
Hirai, Journal of fungi (Basel, Switzerland) 2022 - “...sor -4(DS(r)) A NCU02582 mat A ; sor -4(DS(r)) 1741 FGSC a hxk -2 a NCU00575 mat a ; hxk -2:: Hyg r 15921 FGSC a hxk -2 A NCU00575 mat A ; hxk -2:: Hygr 15920 FGSC a prk -10 a NCU04566 mat a ;...”
- RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa
Li, Biotechnology for biofuels 2021 - “...1 : Table S6), and included some glycolytic proteins such as two hexokinases (EMP-1 and NCU00575), two 6-phosphofructo-2-kinases (NCU01178 and NCU01728), glyceraldehyde 3-phosphate dehydrogenase (GPD-1), and alcohol dehydrogenase-1 (ADH-1). Highly phosphorylated proteins not belonging to these GO terms included pyruvate dehydrogenase E1 component subunit (ACE-2), transketolase...”
- HAM-5 functions as a MAP kinase scaffold during cell fusion in Neurospora crassa
Jonkers, PLoS genetics 2014 - “...or as potential phosphorylation targets of MAK-2 (this study; Table S1 ), including a glucokinase (NCU00575), SUC (pyruvate decarboxylase), an aminotransferase (NCU03500), a trehalose-phosphatase (NCU05041), CAMK-4 calcium/calmodulin-dependent kinase-4, and four hypothetical proteins (NCU00627, NCU00935, NCU006247 and NCU08330) [46] . The identification of MAK-2 phosphorylation targets provides...”
A9S3H4 hexokinase (EC 2.7.1.1) from Physcomitrium patens (see paper)
23% identity, 73% coverage
O93314 Phosphotransferase from Oncorhynchus mykiss
NP_001117721 glucokinase from Oncorhynchus mykiss
24% identity, 56% coverage
- Genomic profiling reveals an alternate mechanism for hepatic tumor promotion by perfluorooctanoic acid in rainbow trout
Tilton, Environmental health perspectives 2008 - “...protein (AF281344; O. mykiss ) 0.10 0.33 1.67 * 0.49 0.45 0.75 OmyOSU875 TC98662 Glucokinase (O93314; O. mykiss ) 0.03 0.60 1.87 * 0.45 0.49 1.92 * OmyOSU873 TC98662 Glucokinase (O93314; O. mykiss ) 0.06 0.58 1.65 * 0.38 0.39 1.66 * OmyOSU249 TC106837 Succinate dehydrogenase,...”
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...(ACM78948), Glossina morsitans morsitans (ADD20426), and Culex quinquefasciatus (XP_001850122). Glucokinases: X. laevis (AAI70499), Oncorhynchus mykiss (NP_001117721), Sparus aurata (AAC33585), H. sapiens (GK1 (NP_000153), GK2 (NP_277042), and GK3 (NP_277043)), Cyprinus carpio (AAC33587), B. taurus (NP_001095772), R. norvegicus (NP_036697), M. musculus (NP_034422), Saccharomyces cerevisiae (AAA53536), Escherichia coli (AP_002988),...”
- Apparent low ability of liver and muscle to adapt to variation of dietary carbohydrate:protein ratio in rainbow trout (Oncorhynchus mykiss).
Skiba-Cassy, The British journal of nutrition 2013 (PubMed)- GeneRIF: In this study, the level of GK mRNA was significantly higher in fish fed the low-carbohydrate (20%)/high protein diet (50%).
- Immunohistochemical localization of glucokinase in rainbow trout brain.
Polakof, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2009 (PubMed)- GeneRIF: This study using immunoreactive techniques, we have demonstrated in those specific areas of the rainbow trout brain previously described as glucosensor the presence of glucokinase in different cell types.
Q3TRM8 Hexokinase-3 from Mus musculus
NP_001028417 hexokinase-3 isoform 1 from Mus musculus
22% identity, 47% coverage
- Proteomic Analysis of Protective Effects of Dl-3-n-Butylphthalide against mpp + -Induced Toxicity via downregulating P53 pathway in N2A Cells
Zhao, Proteome science 2023 - “...with sequence similarity 213, member A \"" 0.013245 Q3TW96 Uap1l1 UDP-N-acetylhexosamine pyrophosphorylase-like protein 1 0.000244 Q3TRM8 Hk3 Hexokinase-3 0.001053 Q3TKR3 Nlrp4c \NACHT, LRR and PYD domains-containing protein 4C \"" 0.004146 Q3TH01 H2-K1 \H-2 class I histocompatibility antigen, K-K alpha chain \"" 0.000264 Q3TGW2 Eepd1 Endonuclease/exonuclease/phosphatase family...”
- Integrative proteomic profiling of lung tissues and blood in acute respiratory distress syndrome.
Gong, Frontiers in immunology 2023 - “...Ltf 3.345018494 2.1092754 P10810 Cd14 3.332715545 3.5366451 P11247 Mpo 3.147273468 1.4498862 P22777 Serpine1 2.971851335 3.4348432 Q3TRM8 Hk3 2.892972242 1.4145415 P31725 S100a9 2.869175648 1.2725338 P41245 Mmp9 2.755070104 1.472634 Q91WP6 Serpina3n 2.561340179 2.0043568 O70138 Mmp8 2.507102878 1.7046071 Q3UZZ4 Olfm4 2.484007335 1.5571941 P12246 Apcs 2.453424421 2.3012683 P15947 Klk1 2.447389196...”
- Proteomics Landscape of Host-Pathogen Interaction in Acinetobacter baumannii Infected Mouse Lung
Li, Frontiers in genetics 2021 - “...2.20 P97369 Ncf4 Neutrophil cytosolic factor 4 1.80 2.19 Q61362 Chil1 Chitinase-like 1 1.71 2.16 Q3TRM8 Hk3 Hexokinase 3 1.43 2.13 P70236 Map2k6 Mitogen-activated protein kinase kinase 6 0.82 1.93 Q61878 Prg2 Proteoglycan 2, bone marrow 0.61 1.91 Q09014 Ncf1 Neutrophil cytosolic factor 1 1.30 1.72...”
- Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes
Chorfa, International journal of molecular sciences 2021 - “...6.45 2 (3.10) 132.20 LDHC * P00342 28,302 7.08 1.30 0.83 3 (17.76) 323.87 HK3 Q3TRM8 102,184 5.23 0.39 7.61 3 (3.15) 163.23 Energy metabolism PRDX5 P99029 20,423 6.00 1.91 4.29 5 (17.60) 313.59 CAR1 P13634 28,550 6.59 1.72 3.44 9 (52.17) 686.90 CAR2 P00920 29,294...”
- A proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages.
Ramirez, PloS one 2021 - “...beta A Central metabolism enzymes eno1/3 P17182 Alpha-enolase A kpym/2 P52480 pyruvate kinase A hxk3/2 Q3TRM8 Hexokinase-3 NA pgam/2 Q9DBJ1 Phosphoglycerate mutase 1 A pgam/3 Q9DBJ1 Phosphoglycerate mutase 1 A pgd/2 Q9DCD0 6-phosphogluconate dehydrogenase, decarboxylating A tpis/3 P17751 Triosephosphate isomerase A Detoxification proteins aldr/2 P45376 Aldose...”
- “...in protein forms for several metabolic enzymes, such as acyl-CoA dehydrogenase (acadl P51174), hexokinase (hxk3 Q3TRM8), phosphoglycerate mutase (PGAM Q9DBJ1), pyruvate kinase (KPYM P52480) or triose phosphate isomerase (TPIS P17751). Indeed, the pathway carbon metabolism appeared in the pathway analyses. We thus tested the activity of...”
- Using a new HSPC senescence model in vitro to explore the mechanism of cellular memory in aging HSPCs.
Dong, Stem cell research & therapy 2021 - “...(model group/young group) Accession Description Gene name Fold change ratio G3UVV4 Hexokinase 1 Hk1 1.2 Q3TRM8 Hexokinase-3 Hk3 1.57 P12382 ATP-dependent 6-phosphofructokinase Pfkl 0.88 Q9WUA3 ATP-dependent 6-phosphofructokinase platelet type Pfkp 1.66 P52480 pyruvate kinase Pkm 1.96 P35486 Pyruvate dehydrogenase E1 component subunit alpha Pdha1 0.95 O70370...”
- PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming.
Feng, International journal of oncology 2020 - “...0.86 - 0.3308 P17710 Hk1 Hexokinase-1 0.91 - 0.1620 O08528 Hk2 Hexokinase-2 0.83 - 0.2196 Q3TRM8 Hk3 Hexokinase-3 NA NA NA Q8VDL4 Adpgk ADP-dependent glucokinase 0.87 - 0.2782 P06745 Gpi Glucose-6-phosphate isomerase 1.05 - 0.2221 P12382 Pfkl ATP-dependent 6-phosphofructokinase, liver type 0.77 - 0.2985 P47857 Pfkm...”
- Host proteome correlates of vaccine-mediated enhanced disease in a mouse model of respiratory syncytial virus infection.
van, Journal of virology 2015 - More
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...M. musculus (AAH54472), D. rerio (AAH45496), and S. pombe HK2 (CAA63488). Hexokinase 3: M. musculus (NP_001028417), R. norvegicus (NP_071515), H. sapiens (NP_002106), Macaca mulatta (XP_001086179), and Nicotiana sylvestris (AAT77513). Invertebrate hexokinases: D. melanogaster (HKA-A (AAF46507), HKC (AAF58160), and HKA-B (AAN09253)), D. simulans (HKA (ABW93133) and HKC...”
PAAG_06172, XP_002791907 glucokinase from Paracoccidioides lutzii Pb01
22% identity, 88% coverage
- Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Rodrigues, Journal of fungi (Basel, Switzerland) 2020 - “...3 XP_002789970 XP_010763627 ATP synthase subunit beta Mitochondrial 0.4385 0.6881 25 0.2767 0.7620 21 8 XP_002791907 XP_010763342 Hexokinase Cytoplasmic 0.3520 0.8065 20 0.3815 0.8873 21 6 XP_015700721 XP_010758595 HSP75-like protein Cytoplasmic 0.8588 0.7374 21 0.8632 0.7372 22 11 XP_002789172 XP_010761260 Uncharacterized protein (M20_dimer domain) Mitochondrial 0.6981...”
- Proteomic profile response of Paracoccidioides lutzii to the antifungal argentilactone
Prado, Frontiers in microbiology 2015 - “...AcuF PAAG_08203 2953.75 0.554 Pyruvate dehydrogenase E1 component alpha subunit PAAG_08295 904.41 0.748 Glucokinase glkA PAAG_06172 746.76 * Phosphoglucomutase PAAG_02011 2057.00 0.482 Phosphoglycerate kinase PAAG_02869 3428.25 0.619 Pyruvate kinase PAAG_06380 9829.55 0.657 Enolase PAAG_00771 39472.25 0.779 Phosphofructokinase subunit PAAG_01583 587.46 * Pyruvate dehydrogenase E1 component beta...”
- Transcriptional and proteomic responses to carbon starvation in Paracoccidioides
Lima, PLoS neglected tropical diseases 2014 - “...51 kDa subunit # ubiquinone ENERGY Glycolysis and gluconeogenesis PAAG_01583 6-phosphofructokinase subunit beta # glycolysis PAAG_06172 glucokinase # glycolysis Tricarboxylic-acid pathway PAAG_00588 fumarate hydratase # TCA cycle PAAG_04597 malate dehydrogenase # TCA cycle Electron transport and membrane-associated energy conservation PAAG_02656 NADH-ubiquinone oxidoreductase 51 kDa subunit #...”
LOC100242358 hexokinase-1 from Vitis vinifera
22% identity, 84% coverage
NP_001193321 hexokinase-3 isoform 3 from Mus musculus
22% identity, 50% coverage
LOC107435837 hexokinase-2, chloroplastic from Ziziphus jujuba
21% identity, 78% coverage
- ZjHXK5 and ZjHXK6 negatively regulate the sugar metabolism of Ziziphus jujuba Mill
Tong, Frontiers in plant science 2024 - “...localization ZjHXK1 LOC107435312 1494 498 Chr1:46678375-46683614+ C4488H7481N1497O1904S282 53.75 1 5.84 32.08 96.18 -0.001 Chloroplast ZjHXK2 LOC107435837 1479 493 Chr6:1387393-1390742- C4428H7376N1482O1868S300 52.98 0 5.52 37.08 93.98 0.034 Chloroplast ZjHXK3 LOC107425017 1473 491 Chr8:12801933-12805086- C4415H7356N1476O1863S263 54.10 1 6.11 35.38 94.09 -0.125 Chloroplast ZjHXK4 LOC107435723 1497 499 Chr12:14728722-14735939- C4463H7428N1500O1887S287...”
G1SD81 hexokinase from Oryctolagus cuniculus
22% identity, 32% coverage
- Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury
Wang, Neural regeneration research 2023 - “...Aldehyde dehydrogenase 0.574050367 0.032825781 800414.6667 459478.3333 A0A5F9DSK5 MECP2 Methyl-CpG-binding protein 2 0.777293892 0.021816219 1161047.333 902475 G1SD81 HK3 Hexokinase 0.520826636 0.04963183 61307421.33 31930538 G1SFZ8 UBQLN1 Ubiquilin 1 0.691693251 0.003030462 4741433.667 3279617.667 G1SK22 RPS27A 40S ribosomal protein S27a 0.643182234 0.001162964 359093.6667 230962.6667 G1TCQ4 GNPDA1 Glucosamine-6-phosphate isomerase 0.66348163 0.024337241...”
M1ALJ6 Phosphotransferase from Solanum tuberosum
21% identity, 82% coverage
- Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease
Xiao, BMC genomics 2020 - “...M1BC24 Phospho-2-dehydro-3-deoxyheptonate aldolase 1.24 0.02 G9IHI3 Apoplastic invertase 0.80 0.00 F2Q9V9 Glyceraldehyde-3-phosphate dehydrogenase 0.82 0.01 M1ALJ6 Phosphotransferase 0.83 0.03 M1BQC2 Pectinesterase 0.82 0.00 Q38JH8 S-adenosylmethionine synthase 2 0.73 0.00 M1CD27 Methylthioribose-1-phosphate isomerase 0.79 0.01 M1BTK3 Potassium transporter 0.74 0.01 M1BM79 Ammonium transporter 0.76 0.00 E14 specific...”
- “...cellular processes were repressed in MA-Phy. For example, apoplastic invertase (G9IHI3), glyceraldehyde-3-phosphate dehydrogenase (F2Q9V9/GAPDH), phosphotransferase (M1ALJ6), and pectinesterase (M1BQC2). The apoplastic invertase is a member of the glycoside hydrolase family that catalyzes the hydrolysis of sucrose into fructose and glucose, (Niki et al., 1998). The GAPDH...”
XP_001086179 hexokinase-3 isoform X1 from Macaca mulatta
23% identity, 47% coverage
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...HK2 (CAA63488). Hexokinase 3: M. musculus (NP_001028417), R. norvegicus (NP_071515), H. sapiens (NP_002106), Macaca mulatta (XP_001086179), and Nicotiana sylvestris (AAT77513). Invertebrate hexokinases: D. melanogaster (HKA-A (AAF46507), HKC (AAF58160), and HKA-B (AAN09253)), D. simulans (HKA (ABW93133) and HKC (EDX07186)), D. yakuba (HKA (EDX02859) and HKC (EDW91124)), Apis...”
HK3 / P52790 hexokinase-3 (EC 2.7.1.1) from Homo sapiens (see 4 papers)
HXK3_HUMAN / P52790 Hexokinase-3; Hexokinase type III; HK III; Hexokinase-C; EC 2.7.1.1 from Homo sapiens (Human) (see paper)
P52790 hexokinase (EC 2.7.1.1) from Homo sapiens (see paper)
NP_002106 hexokinase-3 from Homo sapiens
22% identity, 46% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (PubMed:8717435). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:8717435).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825) - Glioma hexokinase 3 positively correlates with malignancy and macrophage infiltration.
Liang, Metabolic brain disease 2024 (PubMed)- GeneRIF: Glioma hexokinase 3 positively correlates with malignancy and macrophage infiltration.
- Hexokinase 3 enhances myeloid cell survival via non-glycolytic functions.
Seiler, Cell death & disease 2022 - GeneRIF: Hexokinase 3 enhances myeloid cell survival via non-glycolytic functions.
- Hexokinase 3 dysfunction promotes tumorigenesis and immune escape by upregulating monocyte/macrophage infiltration into the clear cell renal cell carcinoma microenvironment.
Xu, International journal of biological sciences 2021 - GeneRIF: Hexokinase 3 dysfunction promotes tumorigenesis and immune escape by upregulating monocyte/macrophage infiltration into the clear cell renal cell carcinoma microenvironment.
- HK3 overexpression associated with epithelial-mesenchymal transition in colorectal cancer.
Pudova, BMC genomics 2018 - GeneRIF: Upregulation of HK3 is associated with EMT in CRC and may be a crucial metabolic adaptation for rapid proliferation, survival, and metastases of CRC cells
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...and S. pombe HK2 (CAA63488). Hexokinase 3: M. musculus (NP_001028417), R. norvegicus (NP_071515), H. sapiens (NP_002106), Macaca mulatta (XP_001086179), and Nicotiana sylvestris (AAT77513). Invertebrate hexokinases: D. melanogaster (HKA-A (AAF46507), HKC (AAF58160), and HKA-B (AAN09253)), D. simulans (HKA (ABW93133) and HKC (EDX07186)), D. yakuba (HKA (EDX02859) and...”
- Human gingiva transcriptome during wound healing.
Wang, Journal of clinical periodontology 2017 (PubMed)- GeneRIF: HK3 expression is significantly upregulated in human masticatory mucosa during wound healing
- High 18F-FDG uptake in PMAH correlated with normal expression of Glut1, HK1, HK2, and HK3.
Cavalcante, Acta radiologica (Stockholm, Sweden : 1987) 2016 (PubMed)- GeneRIF: The transport of glucose across the cell membrane by glucose transporters (GLUTs) and intracellular phosphorylation by hexokinases (HKs) are the initial steps of the glycolytic pathway.
- Identification of key genes affecting disease free survival time of pediatric acute lymphoblastic leukemia based on bioinformatic analysis.
Gao, Blood cells, molecules & diseases 2015 (PubMed)- GeneRIF: Through glycolysis and arachidonic acid metabolism, HK3 and PTGS2 might play important roles in pediatric ALL and its prognosis, and thus, might be potential targets for therapeutic intervention to suppress pediatric ALL.
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- Combined Salivary Proteome Profiling and Machine Learning Analysis Provides Insight into Molecular Signature for Autoimmune Liver Diseases Classification
Guadalupi, International journal of molecular sciences 2023 - “...decreased in AIHp compared to HCs, namely Arginase (P05089), Junction plakoglobin (P14923), Desmoplakin (P15924), Hexokinase-3 (P52790), Desmocollin-1 (Q08554) and Hornerin (Q86YZ3); (ii) one protein increased in AIHp compared to HCs: BPI fold-containing family A member 2 (Q96DR5); (iii) seven proteins increased in PBCp compared to HCs,...”
- “...PBC > HC 0.1899 0.0009 P23528 Cofilin-1 * 0.0679 0.0007 PBC > HC 0.5177 0.0062 P52790 Hexokinase-3 0.0013 AIH < HC 0.0987 0.0819 0.0053 Q08554 Desmocollin-1 * <0.0001 AIH < HC 0.0064 0.0114 <0.0001 Q86YZ3 Hornerin * <0.0001 AIH < HC <0.0001 PBC < HC 0.4824...”
- Oxidized low-density lipoprotein causes ribosome reduction and inhibition of protein synthesis in retinal pigment epithelial cells.
Giorgianni, Biochemistry and biophysics reports 2022 - “...Transketolase 1.7 P37837 Transaldolase 1.8 Q01813 ATP-dependent 6-phosphofructokinase, platelet 1.8 P17858 ATP-dependent 6-phosphofructokinase, liver 2.3 P52790 Hexokinase-3 1.5 P14618 Pyruvate kinase 1.6 Lipid metabolism P37268 Q16850 Squalene synthase Lanosterol 14-alpha demethylase 1.9 2.2 Translation initiation Q9BY44 Q14152 P55010 P60842 P05198 Eukaryotic translation initiation factor 2A Eukaryotic...”
- Radiobiological effects of wound fluid on breast cancer cell lines and human-derived tumor spheroids in 2D and microfluidic culture.
Jeibouei, Scientific reports 2022 - “...O15144, P60709, Q00610, O15511, P02751 hsa05230:Central carbon metabolism in cancer 4 2.919708 0.041693 P16234, P14618, P52790, P11413 Table 2 KEGG pathway of upregulated proteins in tumor margin tissue treated with irradiated WF by DAVID enrichment database. Term Count % P-value Genes hsa01200:Carbon metabolism 11 14.10256 8.49E09...”
- Reprogramming of Trypanosoma cruzi metabolism triggered by parasite interaction with the host cell extracellular matrix.
Mattos, PLoS neglected tropical diseases 2019 - “...T . cruzi -HK (TcCLB.508951.20), T . brucei (Tb927.10.2010) and the three isoforms of human-HK (P52790; P19367; P52789), performed by ClustalW platform ( S2B Fig ). HK/GK activities from MTy and Ty homogenates were measured spectrophotometrically in the presence of a coupling system containing glucose-6-phosphate dehydrogenase....”
- “...(red), PFR (green) and nuclei (blue) in trypomastigotes. (B) Sequence aligment between isoforms of human-HKs (P52790; P19367; P52789) and Tc-HK (Q4D3P5/TcCLB.508951.20). The arrows indicate the phospho-residues and orange box indicates the phosphopeptide identified after phosphoproteome analysis. (TIF) Click here for additional data file. S3 Fig Correlation...”
- Recent advancements in understanding mammalian O-mannosylation
Sheikh, Glycobiology 2017 - “...CRPPA (ISPD) HK O94777 Q9P2X0 Q9UPQ8 A4D126 P19367 P52790 P52789 P34949 O15305 Q9Y5P6 MPI PMM2 GMPPB a Core glycan Subcellular localization Dystroglycanopathy...”
- Pleiotropic effects of 4-hydroxynonenal on oxidative burst and phagocytosis in neutrophils
Chacko, Redox biology 2016 - “...5 Transketolase P06744 12 39.07 6 Phosphoglycerate kinase 1 P06737 11 26.09 7 Glucose-6-phosphate isomerase P52790 9 25.35 8 Glucose-6-phosphate 1-dehydrogenase P00338 16 44.58 9 Glycogen phosphorylase, liver form P07195 6 23.05 10 Transaldolase P40925 2 12.87 11 Hexokinase-3 Q96G03 2 18.95 12 Triosephosphate isomerase P00558...”
- Toward the Proteome of the Human Peripheral Blood Eosinophil.
Straub, Proteomics. Clinical applications 2009 - “...3 260 Rho GDP-dissociation inhibitor 2 P52566 5.10/5.61 23.03/49.74 12 80 6.80E-19 3 261 Hexokinase-3 P52790 5.27/5.57 100.51/166.43 20 26 2.30E-21 1 262 F-actin capping protein alpha-1 subunit P52907 5.45/5.74 33.07/64.26 16 69 1.70E-15 2 263 Biliverdin reductase A precursor P53004 6.06/6.35 33.69/69.46 13 42 5.80E-34...”
- Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages.
Chertova, Journal of virology 2006
G7P6Z7 hexokinase from Macaca fascicularis
23% identity, 47% coverage
3hm8A / P52790 Crystal structure of thE C-terminal hexokinase domain of human hk3
22% identity, 86% coverage
- Ligands: alpha-d-glucopyranose; 6-o-phosphono-beta-d-glucopyranose (3hm8A)
An12g08610 glucokinase glkA-Aspergillus niger from Aspergillus niger
23% identity, 86% coverage
HXKG_ASPNG / Q92407 Glucokinase; Glucose kinase; GLK; Hexokinase glkA; EC 2.7.1.2; EC 2.7.1.1 from Aspergillus niger (see paper)
23% identity, 86% coverage
XP_004135675 hexokinase-1 from Cucumis sativus
24% identity, 86% coverage
XP_002455027 hexokinase-8 from Sorghum bicolor
24% identity, 57% coverage
- Hexose kinases and their role in sugar-sensing and plant development
Granot, Frontiers in plant science 2013 - “...SbHXK3 XP_002459072 Type B/M No Glc sensing role Karve et al. ( 2010 ) SbHXK8 XP_002455027 C Karve et al. ( 2010 ) Wheat (Triticum aestivum) HXK AY974231 ND Controls triose-phosphate/phosphate translocation Sun et al. ( 2006 ) LYCOPHYTES Spike moss ( Selaginella mollendorffii ) SmHXK3...”
XP_006253665 hexokinase-3 isoform X1 from Rattus norvegicus
22% identity, 47% coverage
HXK3_RAT / P27926 Hexokinase-3; Hexokinase type III; HK III; Hexokinase-C; EC 2.7.1.1 from Rattus norvegicus (Rat) (see paper)
NP_071515 hexokinase-3 from Rattus norvegicus
22% identity, 47% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D- fructose 6-phosphate, respectively) (PubMed:5871820). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:5871820).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825) - Analysis of the Effect of Intestinal Ischemia and Reperfusion on the Rat Neutrophils Proteome
Tahir, Frontiers in molecular biosciences 2018 - “...0.392550565 0.309307957 Hypoxanthine-guanine phosphoribosyltransferase EC 2.4.2.8 Q64531 Hprt1 4 0.024144311 0.083055294 0.058910983 Hexokinase-3 EC 2.7.1.1 P27926 Hk3 4 0.016624745 0.117983675 0.10135893 6-phosphofructokinase, liver type EC 2.7.1.11 P12382 Pfkl 4 0.071999766 0.176198903 0.104199137 N-acetylgalactosamine kinase EC 2.7.1.157 Q5XIG6 Galk2 4 0.038659611 0.179037398 0.140377787 Pyruvate kinase PKM EC...”
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...D. rerio (AAH45496), and S. pombe HK2 (CAA63488). Hexokinase 3: M. musculus (NP_001028417), R. norvegicus (NP_071515), H. sapiens (NP_002106), Macaca mulatta (XP_001086179), and Nicotiana sylvestris (AAT77513). Invertebrate hexokinases: D. melanogaster (HKA-A (AAF46507), HKC (AAF58160), and HKA-B (AAN09253)), D. simulans (HKA (ABW93133) and HKC (EDX07186)), D. yakuba...”
B3KRA9 Phosphotransferase from Homo sapiens
22% identity, 72% coverage
HXK3_ARATH / Q9LPS1 Hexokinase-3; Hexokinase-like 1; EC 2.7.1.1 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
AT1G50460 HKL1 (HEXOKINASE-LIKE 1); ATP binding / fructokinase/ glucokinase/ hexokinase from Arabidopsis thaliana
24% identity, 72% coverage
- function: Fructose and glucose phosphorylating enzyme (By similarity). May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol (By similarity). Plays a role in plant growth and development, perhaps by mediating cross-talk between glucose and hormone response pathways (PubMed:19706780). Involved in root hair cell development by mediating certain aspects of cross talk between glucose and ethylene response pathways (PubMed:22366209).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
disruption phenotype: Dwarf plants with reduced roots and leaves growth (PubMed:19706780). Reduced sensitivity of seedlings to glucose repression of development (PubMed:19706780). - HXK, SnRK1, and TOR signaling in plants: Unraveling mechanisms of stress response and secondary metabolism
Eom, Science progress 2024 - “...(Genomic locus) TOR (NCBI accession number) References Arabidopsis AtHXK1 (AT4G29130) AtHXK2 (AT2G19860) AtHXK3 (AT1G47840) AtHXL1 (AT1G50460) AtHXL2 (AT3G20040) AtHXL3 (AT4G37840) AtSnRK1.1 (AT3G01090) AtSnRK1.2 (AT3G29160) AtSnRK1.3 (AT5G39440) NP_175425.2 22 , 106 , 107 Rice OsHXK1 (LOC_Os07g26540.1) a OsHXK2 (LOC_Os05g45590.1) OsHXK3 (LOC_Os01g71320.1) OsHXK4 (LOC_Os07g09890.1) OsHXK5 (LOC_Os05g44760.1) OsHXK6 (LOC_Os01g53930.1)...”
- Investigating the Role of Known Arabidopsis Iron Genes in a Stress Resilient Soybean Line
O'Rourke, International journal of molecular sciences 2024 - “...is involved in plant defense by encoding pathogenesis related proteins; and HKL1 ( Glyma.11G15800 , At1G50460 ), which is a negative regulator of plant growth and a positive regulator of immune responses [ 58 , 59 , 60 , 61 , 62 ]. None of these...”
- Identification of Specific Pathogen-Infected sRNA-Mediated Interactions between Turnip Yellows Virus and Arabidopsis thaliana
Yu, Current issues in molecular biology 2022 - “...469.215 12 13167 22 AT2G18800.1, AT5G58210.3, AT5G58210 CCGAAGCAGAACTGAAGAGCCT 0.400 360.825 11 10126 22 AT1G24620.1, AT1G50460.2, AT1G50460 AGCTAGTGGGGGTTCTGACAC 0.000 53.845 0 1511 21 AT5G45455.1, AT5G08390.1, AT2G47490.1 GATGATCGCCATAGCACTTGAT 0.035 52.235 1 1466 22 AT1G70520.1, AT3G51220.1 CGCTACTATCATCGCCGGGGGT 0.100 52.095 3 1462 22 AT5G35604.1, AT3G19430.2, AT4G13730 TACGGATGAGCAAGTGCTGGACT 0.090 50.830 2...”
- Integrative Analysis of Transcriptomic and Proteomic Changes Related to Cytoplasmic Male Sterility in Spring Stem Mustard (Brassica juncea var. tumida Tsen et Lee)
Shen, International journal of molecular sciences 2022 - “...K00963 UTP--glucose-1-phosphate uridylyltransferase - DOWN DEG BjuB005071 K07024 sucrose-6-phosphatase -- DOWN DEG BjuB047043 K00844 hexokinase AT1G50460 DOWN DEG BjuA021412 K00844 hexokinase - DOWN DEG novel.400 K00850 6-phosphofructokinase 1 AT4G26270 DOWN DEG BjuB040267 K00873 pyruvate kinase AT3G49160 DOWN DAP BjuA041438 K00975 Glucose-1-phosphate adenylyltransferase large subunit 1 AT5G19220...”
- “...K00963 UTP--glucose-1-phosphate uridylyltransferase - DOWN DEG BjuO002531 K01193 beta-fructofuranosidase AT2G36190 DOWN DEG BjuB047043 K00844 hexokinase AT1G50460 DOWN DEG BjuA021412 K00844 hexokinase - DOWN DEG novel.6397 K00850 6-phosphofructokinase 1 AT5G56630 UP DEG BjuB016068 K00850 6-phosphofructokinase 1 - DOWN DAP BjuB038082 K00703 Starch synthase, chloroplastic/amyloplastic AT5G24300 DOWN DAP...”
- Comparative Analysis of the Transcriptomes of Persisting and Abscised Fruitlets: Insights into Plant Hormone and Carbohydrate Metabolism Regulated Self-Thinning of Pecan Fruitlets during the Early Stage
Zhang, Current issues in molecular biology 2021 - “...CIL1347S0008 Glucan endo-1,3-beta-glucosidase 6 3.2.1.39 At5g58090 6.006 5.41 10 32 CIL1359S0017 Hexokinase-3-like [Juglans regia] 2.7.1.1 At1g50460 1.211 3.43 10 3 MSTRG.14644 Hexokinase-3-like isoform X3 2.7.1.1 At1g50460 2.125 2.03 10 7 CIL1459S0007 Hexokinase-3-like isoform X2 2.7.1.1 At1g50460 2.411 5.93 10 7 CIL0282S0003 Hexokinase-1-like isoform X2 2.7.1.1 HXK1...”
- Molecular Targets and Biological Functions of cAMP Signaling in Arabidopsis
Xu, Biomolecules 2021 - “...that is tightly regulated to allow optimal development under non-favorable conditions [ 188 ]); HKL1 (AT1G50460) interacts with the glucose sensor HXK1 and mediates glucose and ethylene responses [ 189 ]; and PMT6/PLT6 (AT4G36670) was suggested to mediate long distance transport of glucose import [ 190...”
- “...AtKUP7 Nitrate NPF5.12 (AT1G72140), NPF7.2/NRT1.8 (AT4G21680), NRT2.6 (AT3G45060), NPF2.7/NAXT1(AT3G45650), CLC-b (AT3G27170) Sugar SWEET16 (AT3G16690), HKL1 (AT1G50460), PMT6/PLT6 (AT4G36670) Lipid MIOX2 (AT2G19800), ITPK3 (AT4G08170), SFH14 (AT5G56160) Light BG1 (AT5G12050), ERD7 (AT2G17840), KNAT4 (AT5G11060), DFL2 (AT4G03400), PKS1 (AT2G02950), PKS2 (AT1G14280), BRN2 (AT1G03457), TEM1 (AT1G25560), BBX30 (AT4G15248) CO 2...”
- Global Transcriptomic Profile Analysis of Genes Involved in Lignin Biosynthesis and Accumulation Induced by Boron Deficiency in Poplar Roots
Su, Biomolecules 2019 - “...1.21 AT5G05170 Potri.006G052600 Pt-CESA4.1 potri.018g088300 Hexokinase 1.38 AT2G26310 potri.001g190400 Hexokinase 1.50 AT4G29130 potri.009g050000 Hexokinase 1.16 AT1G50460 Flavonols Synthase Potri.003G176900 chalcone synthase 1.05 AT5G13930 Potri.003G176800 chalcone synthase 1.03 AT5G13930 Potri.003G176700 chalcone synthase 1.03 AT5G13930 potri.006g219600 chalcone isomerase 1.09 AT2G26310 potri.009g069100 flavonoid 3,5-hydroxylase 1.97 AT5G07990 biomolecules-09-00156-t003_Table 3 Table...”
- Complex Relationships between Chromatin Accessibility, Sequence Divergence, and Gene Expression in Arabidopsis thaliana
Alexandre, Molecular biology and evolution 2018 - “...associated with a DHS deletion or insertion near AT1G50460, AT2G16280, and AT5G54470. cDNA preparations of Bur-0/Col-0 pairs with substantially higher levels of...”
- “...of largest effect size were AT5G54470, AT2G16280, and AT1G50460 (fig. 5C). For example, AT5G54470, known to be induced by cold (Mikkelsen and Thomashow 2009),...”
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A3EYB3 hexokinase (EC 2.7.1.1) from Felis catus (see paper)
23% identity, 78% coverage
HXK4_RAT / P17712 Hexokinase-4; HK4; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-D; EC 2.7.1.1 from Rattus norvegicus (Rat) (see 6 papers)
P17712 hexokinase (EC 2.7.1.1) from Rattus norvegicus (see paper)
23% identity, 78% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively) (PubMed:12513690, PubMed:24187134, PubMed:6477520). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant (PubMed:6477520). Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues (By similarity). Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell (By similarity). In pancreas, plays an important role in modulating insulin secretion (By similarity). In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage (By similarity). Required to provide D-glucose 6- phosphate for the synthesis of glycogen (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:12513690, PubMed:6477520).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
subunit: Monomer (By similarity). Interacts with MIDN; the interaction occurs preferentially at low glucose levels and results in inhibition of hexokinase activity (PubMed:24187134). Interacts with GCKR; leading to sequestration in the nucleus (PubMed:10456334, PubMed:16542652). - iTRAQ-based quantitative proteomics analysis of the hepatoprotective effect of melatonin on ANIT-induced cholestasis in rats.
Wang, Experimental and therapeutic medicine 2021 - “...1.5513 0.0001 P14173 Dopa decarboxylase (DDC) 1.6658 0.0224 P15083 Polymeric immunoglobulin receptor (PIGR) 0.6010 0.0010 P17712 Glucokinase (GCK) 1.9240 0.0404 P17988 Sulfotransferase family 1A member 1 (SULT1A1) 0.4074 0.0080 P18162 Acyl-CoA synthetase long-chain family member 1 (ACSL1) 1.6682 0.0318 P19225 Cytochrome P450, family 2, subfamily c,...”
- Proteomics Analysis of the Spinal Dorsal Horn in Diabetic Painful Neuropathy Rats With Electroacupuncture Treatment.
Yu, Frontiers in endocrinology 2021 - “...0.000162 F7EUK4 T-kininogen 2 Kng1l1 4.212 2.17E-05 0.449 4.18E-05 Q5YIR9 Clec4a1 3.793 1.69E-08 0.225 0.001378 P17712 Glucokinase Gck 3.655 6.97E-07 0.311 0.003456 P01048 T-kininogen 1 Map1 3.457 3.28E-06 0.528 4.31E-05 A0A0H2UHM3 Haptoglobin Hp 2.313 0.035243 0.451 0.000338 M0RAV0 2.242 0.000317 0.646 0.001018 F1M5A4 Katanin p60 ATPase-containing...”
NP_036697 hexokinase-4 isoform 2 from Rattus norvegicus
22% identity, 79% coverage
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...(GK1 (NP_000153), GK2 (NP_277042), and GK3 (NP_277043)), Cyprinus carpio (AAC33587), B. taurus (NP_001095772), R. norvegicus (NP_036697), M. musculus (NP_034422), Saccharomyces cerevisiae (AAA53536), Escherichia coli (AP_002988), and Trypanosoma cruzi GK1 (XP_821474). Figure 4 Three-dimensional (3D) structures of M. nipponense hexokinase (HK) predicted using human hexokinases II as...”
XP_808992 hexokinase, putative from Trypanosoma cruzi
Q4D3P5 Phosphotransferase from Trypanosoma cruzi (strain CL Brener)
22% identity, 87% coverage
- Subcellular proteomics of Trypanosoma cruzi reservosomes
Sant'Anna, Proteomics 2009 - “...to GlcNAc terminal chains N 69 Enolase XP_819700 Glycolysis, phosphopyruvate hydratase activity N 70 Hexokinase XP_808992 Carbohydrate metabolic process N 71 Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB XP_811964 Protein amino acid N-linked glycosylation N 72 Beta galactofuranosyl glycosyltransferase XP_810392 N 73 Lectin XP_807732 Mannose binding N 74 Glucokinase 1...”
- Commentary: Systems Biology Approach to Model the Life Cycle of Trypanosoma cruzi
Carrea, Frontiers in cellular and infection microbiology 2017 - “...Name Uniprot ID of the Homologous Enzyme in T. cruzi Moonlighting Function (Organism) Reference Hexokinase Q4D3P5 Glucose signaling - Porine binding - Apoptosis - Intracellular glucose sensor ( A. thaliana ) Moore et al., 2003 Apoptosis ( E. coli ) Sukumaran et al., 2010 Transcriptional regulation...”
- Systems Biology Approach to Model the Life Cycle of Trypanosoma cruzi
Carrea, PloS one 2016 - “...transporter activity, and Q4E589 has catalytic activity. The other six genes code for: an hexokinase (Q4D3P5), a -1-pyrroline-5-carboxylate dehydrogenase (Q4DRT8), a quinone oxidoreductase (Q4DHH8), a glutamate dehydrogenase (Q4DWV8), a peptidyl-prolyl cis-trans isomerase (Q4E4L9), and a metaciclina II (Q4E2M3). 10.1371/journal.pone.0146947.g006 Fig 6 Life cycle module. (a) Architecture...”
- “...Uniprot ID Putative function k coefficient 195 6382 Q4DTV8 hydrolase activity 3 -0.1805 195 6588 Q4D3P5 hexokinase 3 -0.1805 214 121 Q4DRT8 delta-1-pyrroline-5-carboxylate dehydrogenase 2 0.4251 257 519 Q4DHH8 quinone oxidoreductase 2 0.2256 259 8762 Q4DVU8 transporter activity 2 0.3033 260 4053 Q4E589 catalytic activity 2...”
HXK1_ORYSJ / Q8LH82 Hexokinase-1; EC 2.7.1.1 from Oryza sativa subsp. japonica (Rice) (see 2 papers)
Q8LH82 hexokinase (EC 2.7.1.1) from Oryza sativa (see paper)
NP_001409054 hexokinase-1 from Oryza sativa Japonica Group
24% identity, 84% coverage
C0P8K7 Phosphotransferase from Zea mays
21% identity, 67% coverage
- Comparative Proteomic Analysis of the Response of Maize (Zea mays L.) Leaves to Long Photoperiod Condition
Wu, Frontiers in plant science 2016 - “...64.28%), except that Q9ZR52 (CK2 alpha) were downregulated at the three-leaf stage, as well as C0P8K7 (AtHXK1), B6U4K6 (AtHXK1) and B4F864 (NF-YB12; NF-YB13) in the six-leaf stage. Interestingly, the amount of B4FBL9 ( ELF5 ) decreased at both stages while B4FVS0 (ATFYPP3) and Q1A5Y4 (PHYB; PHYD)...”
- “...additional proteins related to plant circadian rhythms have been identified. overexpression of Arabidopsis hexokinases (AtHXK1, C0P8K7, and B6U4K6) in tomato plants may reduce photosynthesis, inhibit growth and accelerate senescence (Dai et al., 1999 ), These results indicate that the activity of endogenous hexokinase is not a...”
HXK1 / Q42525 fructokinase (EC 2.7.1.4; EC 2.7.1.1) from Arabidopsis thaliana (see 2 papers)
HXK1_ARATH / Q42525 Hexokinase-1; Protein GLUCOSE INSENSITIVE 2; EC 2.7.1.1 from Arabidopsis thaliana (Mouse-ear cress) (see 10 papers)
Q42525 hexokinase (EC 2.7.1.1) from Arabidopsis thaliana (see 4 papers)
AT4G29130 HXK1 (HEXOKINASE 1); ATP binding / fructokinase/ glucokinase/ hexokinase from Arabidopsis thaliana
NP_194642 hexokinase 1 from Arabidopsis thaliana
22% identity, 93% coverage
- function: Fructose and glucose phosphorylating enzyme (PubMed:7610198). May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol (PubMed:16920781). Acts as a sugar sensor which may regulate sugar-dependent gene repression or activation (PubMed:12690200, PubMed:26528314, PubMed:9014361). Mediates the effects of sugar on plant growth and development independently of its catalytic activity or the sugar metabolism (PubMed:12690200, PubMed:9014361). May regulate the execution of program cell death in plant cells (PubMed:16920781). Promotes roots and leaves growth (PubMed:26528314). Together with sugar, is involved in the regulation of the expression of aquaporin genes, and reduces leaf water conductance, to coordinate sugar levels with the loss of water through transpiration (PubMed:28390076). Regulates cell proliferation and expansion early during leaf development (PubMed:30511331). Involved in sucrose-induced leaf growth stimulation independently of GPT2 (PubMed:30511331). May participate to the stimulation of hypocotyl elongation under long-day (LD) conditions (PubMed:34155329). Forms a nuclear complex with CLF and EZA1/SWN to target common glucose- responsive genes and regulate glucose signaling (PubMed:35394700). Is required for CLF- and EZA1/SWN-mediated histone H3 trimethylation on 'Lys-27' (H3K27me3) and glucose-mediated gene repression (PubMed:35394700).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
subunit: Interacts with RPT5B in nucleus. Interacts with RHIP1 (PubMed:26528314). Interacts with KING1 in mitochondria (PubMed:30511331). Interacts with CLF (via SANT domain) and EZA1/SWN (via SANT domain) in nucleus (PubMed:35394700).
disruption phenotype: Plants display a glucose-insensitive phenotype which allows them to grow on high glucose concentration medium (>6% glucose) (PubMed:12690200, PubMed:26528314, PubMed:30511331). Dwarf plants with reduced roots and leaves growth (PubMed:26528314, PubMed:30511331). - HXK, SnRK1, and TOR signaling in plants: Unraveling mechanisms of stress response and secondary metabolism
Eom, Science progress 2024 - “...maize. Plant HXK (Genomic locus) SnRK1 (Genomic locus) TOR (NCBI accession number) References Arabidopsis AtHXK1 (AT4G29130) AtHXK2 (AT2G19860) AtHXK3 (AT1G47840) AtHXL1 (AT1G50460) AtHXL2 (AT3G20040) AtHXL3 (AT4G37840) AtSnRK1.1 (AT3G01090) AtSnRK1.2 (AT3G29160) AtSnRK1.3 (AT5G39440) NP_175425.2 22 , 106 , 107 Rice OsHXK1 (LOC_Os07g26540.1) a OsHXK2 (LOC_Os05g45590.1) OsHXK3 (LOC_Os01g71320.1)...”
- Starch metabolism in guard cells: At the intersection of environmental stimuli and stomatal movement
Dang, Plant physiology 2024 - “...AT1G11260 STP4 Gramene: At3g19930 STP4 Araport: At3g19930 AMY3 Gramene: AT1G69830 AMY3 Araport: AT1G69830 HXK1 Gramene: AT4G29130 HXK1 Araport: AT4G29130 BIK1 Gramene: AT2G39660 BIK1 Araport: AT2G39660 SUS3 Gramene: AT4G02280 SUS3 Araport: AT4G02280 KAT1 Gramene: AT1G04710 KAT1 Araport: AT1G04710 SLAH3 Gramene: AT5G24030 SLAH3 Araport: AT5G24030 adg1 Gramene: AT5G48300...”
- L-gulono-γ-lactone Oxidase, the Key Enzyme for L-Ascorbic Acid Biosynthesis
Gad, Current issues in molecular biology 2024 - “...enzymes responsible for vitamin C biosynthesis in A . thaliana from glucose are hexokinase 1 (At4g29130, HXK1), phosphoglucose isomerase (At4g24620, PGI), phosphomannose isomerase 2 (At1g67070, DIN9, PMI2), phosphomannomutase (At2g45790, PMM), GDP-mannose pyrophosphorylase 1 (At2g39770, VTC1, GMP1), GDP-d-mannose epimerase (At5g28840, GME), GDP-L-galactose phosphorylase (At4g26850, VTC2, GGP), L-galactose...”
- Changes in cell wall composition due to a pectin biosynthesis enzyme GAUT10 impact root growth
Dash, Plant physiology 2023 - “...AT1G01820 GAUT14 Gramene: AT5G15470 GAUT14 Araport: AT5G15470 mur1 Gramene: AT3G51160 mur1 Araport: AT3G51160 HXK1 Gramene: AT4G29130 HXK1 Araport: AT4G29130 GAUT13 Gramene: AT3G01040 GAUT13 Araport: AT3G01040 MUR3 Gramene: AT2G20370 MUR3 Araport: AT2G20370 References Baluska F , Volkmann D , Barlow PW . Specialized zones of development in...”
- Three consecutive cytosolic glycolysis enzymes modulate autophagic flux
Lee, Plant physiology 2023 - “...paper: ATG1 Gramene: AT3G51790 ATG1 Araport: AT3G51790 GAPC1 Gramene: AT3G04120 GAPC1 Araport: AT3G04120 HXK1 Gramene: AT4G29130 HXK1 Araport: AT4G29130 PGK3 Gramene: AT1G79550 PGK3 Araport: AT1G79550 ATG101 Gramene: AT5G66930 ATG101 Araport: AT5G66930 CyTPI Gramene: AT3G55440 CyTPI Araport: AT3G55440 ATG6 Gramene: AT3G61710 ATG6 Araport: AT3G61710 References Avin-Wittenberg T...”
- Probing the physiological role of the plastid outer-envelope membrane using the oemiR plasmid collection
Schwenkert, G3 (Bethesda, Md.) 2023 - “...TGATTATACGGCAGGAGGCAG Sun et al. (2011) Froehlich et al. (2003) Other 24 HXK1 Glucose-responsive sensor hexokinase At4g29130 TATTACCGAAAAATGGCGCTG Jang et al. (1997) Simm et al. (2013) Signal anchored 25 CRL OEP80 insertion At5g51020 TTATAGTCGTCAAATGCGCTC Asano et al. (2004) , Yoshimura et al (2023) ( Simm et al....”
- Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis
Rudenko, Antioxidants (Basel, Switzerland) 2023 - “...At5g27380 Glutathione Synthetase 2, ATGSH2, GSH2, GSHB Binding -glutamylcysteine and glycine together to form glutathione At4g29130 Hexokinase 1, HXK1, ATHXK1, GIN2 Hexose phosphorylation activity At2g19860 Hexokinase 2, HXK2, ATHXK2 At1g47840 Hexokinase 3, HXK3 At4g24620 Phosphoglucose Isomerase, PGI, Glucose-6-phosphate isomerase Transformation of d-glucose-6-phosphate into d-fructose-6-phosphate At3g02570 At1g67070...”
- Sugar status in preexisting leaves determines systemic stomatal development within newly developing leaves
Bao, Proceedings of the National Academy of Sciences of the United States of America 2023 - “...Genome Initiative or GenBank/EMBL databases under the following accession numbers: AT3G20770 (EIN3) ( 46 ), AT4G29130 (HXK1) ( 47 ), and AT1G22710 (SUC2) ( 48 ).All study data are included in the article and/or SIAppendix . 1 J. Sheen , Sugars as signaling molecules . Curr....”
- More
- Complex genetic interaction between glucose sensor HXK1 and E3 SUMO ligase SIZ1 in regulating plant morphogenesis.
Rawat, Plant signaling & behavior 2024 - GeneRIF: Complex genetic interaction between glucose sensor HXK1 and E3 SUMO ligase SIZ1 in regulating plant morphogenesis.
- The role of HEXOKINASE1 in Arabidopsis leaf growth.
Van, Plant molecular biology 2019 (PubMed)- GeneRIF: HXK1 regulates cell proliferation and expansion early during leaf development, and that HXK1 is involved in sucrose-induced leaf growth stimulation independent of GPT2.
- Subcellular dynamics of proteins and metabolites under abiotic stress reveal deferred response of the Arabidopsis thaliana hexokinase-1 mutant gin2-1 to high light.
Küstner, The Plant journal : for cell and molecular biology 2019 (PubMed)- GeneRIF: Combining proteome and metabolome analysis at subcellular resolution revealed that the hexokinase-1 mutant of Arabidopsis, gin2-1 , cannot respond adequately to sudden changes of light intensity, thus explaining its high light sensitivity.
- Glucose triggers stomatal closure mediated by basal signaling through HXK1 and PYR/RCAR receptors in Arabidopsis.
Li, Journal of experimental botany 2018 - GeneRIF: Glucose-triggered stomatal closure may be dependent on basal signaling through PYR/RCAR receptors and hexokinase1 (HXK1).
- Sugar and hexokinase suppress expression of PIP aquaporins and reduce leaf hydraulics that preserves leaf water potential.
Kelly, The Plant journal : for cell and molecular biology 2017 (PubMed)- GeneRIF: HXK1 affects the expression of PIPaquaporins genes and reduce leaf water conductance, to coordinate sugar levels with the loss of water through transpiration.
- Measuring whole plant CO2 exchange with the environment reveals opposing effects of the gin2-1 mutation in shoots and roots of Arabidopsis thaliana.
Brauner, Plant signaling & behavior 2015 - GeneRIF: The CO2 metabolism of the gin2-1 mutation in A. thaliana is reported.
- Biochemical and structural study of Arabidopsis hexokinase 1.
Feng, Acta crystallographica. Section D, Biological crystallography 2015 (PubMed)- GeneRIF: The structure of HXK1 was determined both in its inactive unliganded form and in its active glucose-bound form at resolutions of 1.8 and 2.0 A, respectively.
- Involvement of Arabidopsis Hexokinase1 in Cell Death Mediated by Myo-Inositol Accumulation.
Bruggeman, The Plant cell 2015 - GeneRIF: role in cell death mediated by myo-inositol accumulation
- More
- Disclosing proteins in the leaves of cork oak plants associated with the immune response to Phytophthora cinnamomi inoculation in the roots: A long-term proteomics approach
Coelho, PloS one 2021 - “...0.041 -0.3 Fructose-bisphosphate aldolase 8, cytosolic ALFC8/FBA8 F4KDZ4 2.902 1.688 0.026 -0.3 Malate dehydrogenase F4KDZ4/PMDH2 Q42525 0.553 0.340 0.026 -0.3 Hexokinase-1 HXK1 P27140 7.947 6.602 0.041 -0.2 Beta carbonic anhydrase 1, chloroplastic BCA1 A0A1P8BG37 3.388 2.750 0.041 -0.2 Photosystem II stability/assembly factor, chloroplast A0A1P8BG37 Q9SAU2 1.863...”
- “...catabolic process (6) 6 O49299 Probable phosphoglucomutase, cytoplasmic 1 (PGMC1/PGM1) Cytoplasm Carbohydrate metabolism 0.2 4.6 Q42525 Hexokinase-1 (HXK1) Cytoplasm Nucleous Glycolysis; Stress signalling -0.3 4.6 Q9SAU2 D-ribulose-5-phosphate-3-epimerase (Q9SAU2/RPE) Chloroplast Photosynthesis -0.2 Cellular metabolic compound salvage (3) 3 P10795 Ribulose bisphosphate carboxylase small chain 1A (RBS1A/RBCS1A) Chloroplast...”
- The invertebrate Caenorhabditis elegans biosynthesizes ascorbate
Patananan, Archives of biochemistry and biophysics 2015 - “...Uniprot ID Reaction C. elegans Protein % AA identity E-value Mutual best hit Hexokinase * Q42525 D-glucoseD-glucose-6-P F14B4.2 * 37% 1310 79 Yes Phosphoglucose isomerase * P34795 D-glucose-6-PD-fructose-6-P Y87G2A.8 ** 47% 1310 151 Yes Phosphomannose isomerase * Q9M884 D-fructose-6-PD-mannose-6-P ZK632.4 *** 34% 6310 71 Yes Phosphomannomutase...”
GRMZM2G171373 uncharacterized protein LOC100279587 from Zea mays
21% identity, 65% coverage
- Involvement of Phospholipase C in Photosynthesis and Growth of Maize Seedlings
Wei, Genes 2022 - “...[ 34 , 40 ]. We found increased gene expression of pyruvate kinases, such as GRMZM2G171373 and GRMZM2G150098 ( Table S3 ). This suggests that inhibition of phospholipase C activity has less effect on the glycolytic process. In a mitochondrion, PC and PE are the major...”
- Using high-throughput multiple optical phenotyping to decipher the genetic architecture of maize drought tolerance
Wu, Genome biology 2021 - “...), GRMZM2G045404 ( ibr5 ), sugar metabolism: GRMZM2G111324 ( ogh17 ), GRMZM2G318780 ( scs3 ), GRMZM2G171373 ( hk1 ) and peroxide metabolism: GRMZM2G162688 ( sip ), GRMZM5G872256 ( gs1 ). In addition, many significant peaks were repeatedly detected for genes encoding TFs that regulate multiple biological...”
- Genome-Wide Association Study of Maize Aboveground Dry Matter Accumulation at Seedling Stage
Lu, Frontiers in genetics 2020 - “...of these, 18 genes ( GRMZM2G105571 , Zm00001d027469 , Zm00001d043622 , GRMZM2G353553 , GRMZM2G167856 , GRMZM2G171373 , GRMZM2G092120 , CKX10 , GRMZM2G035217 , GRMZM2G128644 , GRMZM2G314064 , GRMZM2G407825 , GRMZM6G865522 , GRMZM2G045732 , pco092737 , Zm00001d002111 , GRMZM2G042412 , and GRMZM2G061537 ) were associated with a...”
- “...[ GRMZM2G105571 ( Li et al., 2018 ), GRMZM2G167856 ( Li et al., 2016 ), GRMZM2G171373 ( Aguilera-Alvarado et al., 2019 ), CKX10 ( Smehilova et al., 2009 ), GRMZM2G128644 ( Song et al., 2016 ), GRMZM2G314064 ( Jiang et al., 2012 ), GRMZM2G407825 ( Yan...”
I1RGY2 hexokinase (EC 2.7.1.1) from Fusarium graminearum (see paper)
24% identity, 72% coverage
Q8ST54 hexokinase (EC 2.7.1.1) from Trypanosoma cruzi (see paper)
22% identity, 87% coverage
LOC101215511 hexokinase-3 from Cucumis sativus
26% identity, 56% coverage
- The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber
Zhao, Plants (Basel, Switzerland) 2021 - “...LOC101213767 CGTGTGCTAAGGAAGGCGGAAG CsSuSy1-R CAGTGTCACCCCACCCTCTCTC CsSuSy6-F LOC101216865 TCCAACCGCCACAACTTCATCAC CsSuSy6-R CCATTCCCACTCTGCCCAAGC CsHK1-F LOC101218300 CGCCATGACCGTCGAGATGC CsHK1-R TTTGTACCGCCGAGATCCAATGC CsHK3-F LOC101215511 CACGGTCCTAGTCAGTCGGAGAG CsHK3-R GCCATAGCATCAACCACCTGTCTC CsUDP1-F LOC101206505 TCCAGAGTTCCTTGCTGAGGGTAC CsUDP1-R AAGCCTGAATTGCCTTGAGACCATC CsUDP1-like-F LOC116401645 AGTTAATGCCATTTCCGCCCTCTG CsUDP1-like-R TCTTGTATCCGTACCAACCGAATGC CsG6P1-F LOC101222586 AGGTGCGATTGCTAATCCAGATGAG CsG6P1-R TGCGACTTCAAGAACGAGTTAGGTG CsG6P1-like-F LOC101210696 AGGGTGGAGGTTTAGGGTTTAGGG CsG6P1-like-R GCCGCTCGTTCATTCCATTGTTC a NCBI database...”
LOC107435312 hexokinase-1-like from Ziziphus jujuba
22% identity, 85% coverage
- ZjHXK5 and ZjHXK6 negatively regulate the sugar metabolism of Ziziphus jujuba Mill
Tong, Frontiers in plant science 2024 - “...(kDa) TMs PI Instability Index Aliphatic Index Grand Average of Hydropathicity Subcellular predictive localization ZjHXK1 LOC107435312 1494 498 Chr1:46678375-46683614+ C4488H7481N1497O1904S282 53.75 1 5.84 32.08 96.18 -0.001 Chloroplast ZjHXK2 LOC107435837 1479 493 Chr6:1387393-1390742- C4428H7376N1482O1868S300 52.98 0 5.52 37.08 93.98 0.034 Chloroplast ZjHXK3 LOC107425017 1473 491 Chr8:12801933-12805086- C4415H7356N1476O1863S263...”
FPSE_02221 hypothetical protein from Fusarium pseudograminearum CS3096
23% identity, 72% coverage
- Transcriptomic Profiling of Fusarium pseudograminearum in Response to Carbendazim, Pyraclostrobin, Tebuconazole, and Phenamacril
Zhang, Journal of fungi (Basel, Switzerland) 2023 - “...(NADP-dependent alcohol dehydrogenase 7, ADH7 ), FPSE_11514 (enolase, ENO ), FPSE_09735 (pyruvate decarboxylase, PDC ), FPSE_02221 (hexokinase, HK ), FPSE_07501 (pyruvate kinase, PK ), FPSE_11514 (acetyl-coenzyme A synthetase, ACS ), and FPSE_07708 (transketolase, TKLIA ) may play important roles in these carbon pathways. Amino sugar and...”
- “...1.61 1.32 1.40 - 1 FPSE_07681 ACS Acetyl-coenzyme A synthetase 3.09 - 1.84 - 1 FPSE_02221 HK Hexokinase 3.55 - - - 1 FPSE_09736 ppsA Phosphoenolpyruvate synthase 3.69 - - 2.25 1 FPSE_07708 TKLlA, Transaldolase 1.26 - - - 1 FPSE_09193 CHS8 Chitin synthase 8 -...”
F2CW03 Phosphotransferase from Hordeum vulgare subsp. vulgare
22% identity, 79% coverage
LOC103837987 hexokinase-1 from Brassica rapa
23% identity, 86% coverage
LOC103449780 hexokinase-2-like from Malus domestica
23% identity, 86% coverage
LOC106439029 hexokinase-3 from Brassica napus
24% identity, 85% coverage
- Comparative Cytological and Transcriptome Analyses of Anther Development in Nsa Cytoplasmic Male Sterile (1258A) and Maintainer Lines in Brassica napus Produced by Distant Hybridization
Xing, International journal of molecular sciences 2022 - “...levels in the pre-anther abortion period, namely LOC106421218 , LOC106399638 , LOC106346734 , LOC106440633 , LOC106439029 , LOC106413118 , and LOC106356810 ( Figure 9 C). Seven genes in Class V had abnormally low expression levels, namely LOC106422956 , LOC106454372 , LOC106350934 , LOC106428191 , LOC106382995 ,...”
NP_001315646 hexokinase-1-like from Malus domestica
23% identity, 86% coverage
LOC103832872 hexokinase-3 from Brassica rapa
24% identity, 76% coverage
NP_001095772 hexokinase-4 from Bos taurus
22% identity, 78% coverage
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...(AAC33585), H. sapiens (GK1 (NP_000153), GK2 (NP_277042), and GK3 (NP_277043)), Cyprinus carpio (AAC33587), B. taurus (NP_001095772), R. norvegicus (NP_036697), M. musculus (NP_034422), Saccharomyces cerevisiae (AAA53536), Escherichia coli (AP_002988), and Trypanosoma cruzi GK1 (XP_821474). Figure 4 Three-dimensional (3D) structures of M. nipponense hexokinase (HK) predicted using human...”
Afu6g02230, XP_747854 glucokinase GlkA, putative from Aspergillus fumigatus Af293
23% identity, 86% coverage
- Aspergillus fumigatus Strain-Specific Conidia Lung Persistence Causes an Allergic Broncho-Pulmonary Aspergillosis-Like Disease Phenotype
Jones, mSphere 2021 - “...nucleosome assembly protein Afu5g09210 2 Autophagic (vacuolar) serine protease Afu5g13300 2 Putative extracellular aspartic endopeptidase Afu6g02230 2 Putative glucokinase Afu6g02280 2 Allergen Asp f 3 Afu6g09690 2 Glutathione S -transferase gliG Afu6g09740 2 Thioredoxin reductase gliT Afu8g07080 2 Putative secreted metalloprotease Afu1g02820 1 Putative NADH-quinone oxidoreductase...”
- Identifying candidate Aspergillus pathogenicity factors by annotation frequency
Pennerman, BMC microbiology 2020 - “...A. fumigatus Af293 (XP_746328.1, UPF0075 family protein; XP_747575.1, polysaccharide deactylase family protein; XP_747679.1, putative hexokinase; XP_747854.1, putative glucokinase GlkA; XP_747946.1, putative LysM domain protein; XP_748231.1, hypothetical protein AFUA_5G0110; XP_749104.1, putative hexokinase; XP_749181.1, putative hexokinase; XP_749720.1, putative hexokinase Kxk; XP_751374.1, putative C6 transcription factor; XP_752897.1, polysaccharide deactylase...”
- “...to the initial level. Non-significant upregulation was commonly observed for the gene encoding the glucokinase XP_747854 ( p -value=0.18). Genes encoding these proteins were not in the PHI-base. Fig. 4 Gene expression during co-culture with human immune cells. Expression of A. fumigatus (Af) amino-sugar processing genes...”
- Transcriptomic and proteomic analyses of the Aspergillus fumigatus hypoxia response using an oxygen-controlled fermenter
Barker, BMC genomics 2012 - “...tended to be transcriptionally reduced or unchanged in response to hypoxia, whereas the two glucokinases (Afu6g02230 and Afu2g16330) were induced at later time points when glucose levels began to drop. Thus, it is unclear whether these glucose responsive transcripts were altered due to hypoxia or changes...”
- “...the glucose level in the culture medium at later time-points. We chose to look at Afu6g02230 ( glkA ) and Afu2g05910 ( hxkA ) as these are thought to be the main active enzymes in A. fumigatus for this part of glycolysis [ 32 ]. As...”
HXK2_ORYSJ / Q2KNB9 Hexokinase-2; Hexokinase-3; EC 2.7.1.1 from Oryza sativa subsp. japonica (Rice) (see paper)
Q2KNB9 hexokinase (EC 2.7.1.1) from Oryza sativa (see paper)
23% identity, 63% coverage
- function: Fructose and glucose phosphorylating enzyme.
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
F1KVA2 Phosphotransferase from Ascaris suum
23% identity, 89% coverage
3idhA / P35557 Human pancreatic glucokinase in complex with glucose (see paper)
24% identity, 48% coverage
- Ligand: alpha-d-glucopyranose (3idhA)
NP_277042 hexokinase-4 isoform 2 from Homo sapiens
24% identity, 48% coverage
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...Glucokinases: X. laevis (AAI70499), Oncorhynchus mykiss (NP_001117721), Sparus aurata (AAC33585), H. sapiens (GK1 (NP_000153), GK2 (NP_277042), and GK3 (NP_277043)), Cyprinus carpio (AAC33587), B. taurus (NP_001095772), R. norvegicus (NP_036697), M. musculus (NP_034422), Saccharomyces cerevisiae (AAA53536), Escherichia coli (AP_002988), and Trypanosoma cruzi GK1 (XP_821474). Figure 4 Three-dimensional (3D)...”
Afu2g00450 hexokinase, putative from Aspergillus fumigatus Af293
22% identity, 78% coverage
GCK / P35557 glucokinase (EC 2.7.1.1) from Homo sapiens (see 13 papers)
HXK4_HUMAN / P35557 Hexokinase-4; HK4; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-D; EC 2.7.1.1 from Homo sapiens (Human) (see 36 papers)
P35557 hexokinase (EC 2.7.1.1) from Homo sapiens (see 11 papers)
Q53Y25 hexokinase (EC 2.7.1.1) from Homo sapiens (see paper)
NP_000153 hexokinase-4 isoform 1 from Homo sapiens
24% identity, 48% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively) (PubMed:11916951, PubMed:15277402, PubMed:17082186, PubMed:18322640, PubMed:19146401, PubMed:25015100, PubMed:7742312, PubMed:8325892). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant (By similarity). Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues (PubMed:11916951, PubMed:15277402, PubMed:18322640, PubMed:25015100, PubMed:8325892). Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell (By similarity). In pancreas, plays an important role in modulating insulin secretion (By similarity). In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage (By similarity). Required to provide D-glucose 6-phosphate for the synthesis of glycogen (PubMed:8878425). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:7742312).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
subunit: Monomer (PubMed:15016359, PubMed:19362831, PubMed:23957911). Interacts with MIDN; the interaction occurs preferentially at low glucose levels and results in inhibition of hexokinase activity (PubMed:24187134). Interacts with GCKR; leading to sequestration in the nucleus (PubMed:10456334). - A Case of Mody 2 - Associated Hyperglycemia Diagnosed as Gestational Diabetes.
Chakarova, Balkan journal of medical genetics : BJMG 2022 - “...valine with methionine at codon 62 (V62M) of the amino acid sequence of the protein (NP_000153.1). This variant (rs1064793998) is extremely rare and is not reported in large population databases, such as gnomAD, ExAC and GenomeAsia 100K. V62M in GCK has been found, however, in multiple...”
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...quinquefasciatus (XP_001850122). Glucokinases: X. laevis (AAI70499), Oncorhynchus mykiss (NP_001117721), Sparus aurata (AAC33585), H. sapiens (GK1 (NP_000153), GK2 (NP_277042), and GK3 (NP_277043)), Cyprinus carpio (AAC33587), B. taurus (NP_001095772), R. norvegicus (NP_036697), M. musculus (NP_034422), Saccharomyces cerevisiae (AAA53536), Escherichia coli (AP_002988), and Trypanosoma cruzi GK1 (XP_821474). Figure 4...”
- The liver proteome of individuals with a natural UGT2B17 complete deficiency.
Rouleau, Scientific reports 2025 - MSC secretome from amniotic fluid halts IL-1β and TNF-α inflammation via the ERK/MAPK pathway, promoting cartilage regeneration in OA in vitro.
Klaymook, Journal of stem cells & regenerative medicine 2024 - Comparison of Variants of Uncertain Significance in Three Regions of the Human Glucokinase Protein Using In Vitro and In Silico Analyses.
Gay, Cureus 2024 - “...these specific variants in patients. Materials and methods GCK variant plasmid construction The FASTA file P35557 obtained from UniProt [ 19 ] was used as the template for the cDNA [ 20 ]. Previous publications show that GCK tolerates an amino-terminal tag [ 21 , 22...”
- A Network Pharmacology Method Combined with Molecular Docking Verification to Explore the Therapeutic Mechanisms Underlying Simiao Pill Herbal Medicine against Hyperuricemia
Qian, BioMed research international 2023 - “...Q96P20 31 RAPGEF4 Q8WZA2 71 APOE P02649 32 KHK P50053 72 ACAT1 P24752 33 GCK P35557 73 APOB P04114 34 NGF P01138 74 COG2 Q14746 35 LCN2 P80188 75 TNFRSF11B O00300 36 NPPA P01160 76 ALDH2 P05091 37 NPPB P16860 77 G6PD P11413 38 HNF1A P20823...”
- Preclinical Evaluation of a Novel Small Molecule LCC-21 to Suppress Colorectal Cancer Malignancy by Inhibiting Angiogenic and Metastatic Signatures.
Mokgautsi, Cells 2023 - “...kinase 1 Catenin 1 CDK1 CTNNB1 P06493 P36861 CHEMBL308 CHEMBL309 Kinase Kinase MYC proto-oncogene MYC P35557 CHEMBL3820 Enzyme Matrix metalloproteinase 7 MMP7 P14780 CHEMBL321 Enzyme cells-12-00266-t002_Table 2 Table 2 Analytical summary table showing interactions of LCC-21 with VEGFA/CTNNB1/MMP7/CD44 . VEGFA-LCC-21 Complex ( = 8.1 kcal/mol) CTNNB1-LCC-21...”
- Suppression of Pathological Ocular Neovascularization by a Small Molecular Multi-Targeting Kinase Inhibitor, DCZ19903
Ding, Translational vision science & technology 2022 - “...P35916 CHEMBL1955 Kinase 0.082221517 ACACB O00763 CHEMBL4829 Ligase 0.082221517 PIK3CA P42336 CHEMBL4005 Enzyme 0.082221517 GCK P35557 CHEMBL3820 Enzyme 0.082221517 TACR2 P21452 CHEMBL2327 Family A G protein-coupled receptor 0.082221517 KDR P35968 CHEMBL279 Kinase 0.082221517 JAK1 P23458 CHEMBL2835 Kinase 0.082221517 MAPKAPK2 P49137 CHEMBL2208 Kinase 0.082221517 ADORA2A P29274 CHEMBL251...”
- Exploring the mechanism of Alisma orientale for the treatment of pregnancy induced hypertension and potential hepato-nephrotoxicity by using network pharmacology, network toxicology, molecular docking and molecular dynamics simulation
Liao, Frontiers in pharmacology 2022 - “...131 Nuclear receptor subfamily 1 group I member 3 NR1I3 Q14994 Toxic 54 Hexokinase-4 GCK P35557 Treat 132 Serine/threonine-protein kinase pim-1 PIM1 P11309 Toxic 55 Glutathione S-transferase Mu 1 GSTM1 P09488 Treat 133 Serine/threonine-protein kinase PLK1 PLK1 P53350 Toxic 56 Glutathione S-transferase P GSTP1 P09211 Treat...”
- Use of Network Pharmacology to Explore the Mechanism of Gegen (Puerariae lobatae Radix) in the Treatment of Type 2 Diabetes Mellitus Associated with Hyperlipidemia
Yuan, Evidence-based complementary and alternative medicine : eCAM 2021 - “...Fatty acid-binding protein, adipocyte 2539 G6PD P11413 Glucose-6-phosphate 1-dehydrogenase 2641 GCG P01275 Pro-glucagon 2645 GCK P35557 Hexokinase-4 2690 GHR P10912 Growth hormone receptor 3156 HMGCR P04035 3-Hydroxy-3-methylglutaryl-Coenzyme A reductase 3162 HMOX1 P09601 Heme oxygenase 1 3290 HSD11B1 P28845 Corticosteroid 11-beta-dehydrogenase isozyme 1 3553 IL1B P01584 Interleukin-1...”
- More
F4YGN8 Phosphotransferase (Fragment) from Hordeum vulgare subsp. vulgare
23% identity, 75% coverage
TRIREDRAFT_80231 uncharacterized protein from Trichoderma reesei QM6a
23% identity, 84% coverage
HXK4_MOUSE / P52792 Hexokinase-4; HK4; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-D; EC 2.7.1.1 from Mus musculus (Mouse) (see 6 papers)
NP_034422 hexokinase-4 isoform 1 from Mus musculus
23% identity, 49% coverage
- function: Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively) (PubMed:8530440). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant (By similarity). Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues (PubMed:8530440, PubMed:9867845). Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell (PubMed:8530440, PubMed:9867845). In pancreas, plays an important role in modulating insulin secretion (PubMed:8530440). In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage (PubMed:9867845). Required to provide D-glucose 6-phosphate for the synthesis of glycogen (PubMed:9867845). Mediates the initial step of glycolysis by catalyzing phosphorylation of D- glucose to D-glucose 6-phosphate (By similarity).
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
catalytic activity: D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+) (RHEA:11028)
subunit: Monomer (By similarity). Interacts with MIDN; the interaction occurs preferentially at low glucose levels and results in inhibition of hexokinase activity (By similarity). Interacts with GCKR; leading to sequestration in the nucleus (PubMed:10713097).
disruption phenotype: Embryonic lethality caused by the absence of hexokinase activity (PubMed:7665557). Conditional deletion in pancreatic beta-cells causes severe diabetes shortly after birth leading to lethality within a week (PubMed:8530440, PubMed:9867845). Deficient islets show defective insulin secretion in response to glucose (PubMed:8530440). Conditional deletion in liver leads to mild hyperglycemia; however, mice display pronounced defects in both glycogen synthesis and glucose turnover rates during a hyperglycemic clamp (PubMed:9867845). - (Re-)Viewing Role of Intracellular Glucose Beyond Extracellular Regulation of Glucose-Stimulated Insulin Secretion by Pancreatic Cells
Firdos,, ACS omega 2024 - “...kDa subunit a isoform 4 840 H. sapiens Q9HBG4 GCK hexokinase 4 465 M. musculus P52792 GCK hexokinase 4 465 H. sapiens 1V4S /P35557 SLC2A2 solute carrier family 2, facilitated glucose transporter member 2 523 M. musculus P14246 SLC2A2 solute carrier family 2, facilitated glucose transporter...”
- Role of Fas-associated death domain-containing protein (FADD) phosphorylation in regulating glucose homeostasis: from proteomic discovery to physiological validation
Yao, Molecular & cellular proteomics : MCP 2013 - “...liver G6PT HXK4 PPCKC TPI1 KPYR ALDOA GYS2 P35576 P52792 Q9Z2V4 P17751 P53657 P05064 Q8VCB3 40,473 52,089 69,355 32,192 62,309 39,356 80,871 -1.97 4.65 -2.34...”
- Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
Sun, International journal of molecular sciences 2017 - “...(NP_277042), and GK3 (NP_277043)), Cyprinus carpio (AAC33587), B. taurus (NP_001095772), R. norvegicus (NP_036697), M. musculus (NP_034422), Saccharomyces cerevisiae (AAA53536), Escherichia coli (AP_002988), and Trypanosoma cruzi GK1 (XP_821474). Figure 4 Three-dimensional (3D) structures of M. nipponense hexokinase (HK) predicted using human hexokinases II as template models. Different...”
- Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-beta signaling
Cummins, Biochimica et biophysica acta 2010 - “...[ 48 ] NP_038523 carnitine palmitoyltransferase 1A (CPT1A) 3.3 IDDM [ 49 , 50 ] NP_034422 Glucokinase (GCK) MODY, NIDDM [ 51 56 ] NP_034404 glycerol-3-phosphate dehydrogenase 2 (GPD2) 4.95 NIDDM [ 57 ] NP_038848 hexokinase 2 (HK2) 5.28 Experimentally-induced diabetes, regulates D-glucose, regulated by glucose-6-phosphate...”
HXK10_ORYSJ / Q2KNB5 Hexokinase-10; Hexokinase-7; EC 2.7.1.1 from Oryza sativa subsp. japonica (Rice) (see paper)
Q2KNB5 hexokinase (EC 2.7.1.1) from Oryza sativa (see paper)
25% identity, 54% coverage
- function: Fructose and glucose phosphorylating enzyme.
catalytic activity: a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+) (RHEA:22740)
catalytic activity: D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+) (RHEA:16125)
catalytic activity: D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+) (RHEA:17825)
XP_006514507 hexokinase-4 isoform X2 from Mus musculus
23% identity, 49% coverage
- MAP4K3/GLK inhibits Treg differentiation by direct phosphorylating IKKβ and inducing IKKβ-mediated FoxO1 nuclear export and Foxp3 downregulation.
Chi, Theranostics 2022 - GeneRIF: MAP4K3/GLK inhibits Treg differentiation by direct phosphorylating IKKbeta and inducing IKKbeta-mediated FoxO1 nuclear export and Foxp3 downregulation.
- Biphasic dynamics of beta cell mass in a mouse model of congenital hyperinsulinism: implications for type 2 diabetes.
Tornovsky-Babeay, Diabetologia 2021 - GeneRIF: Biphasic dynamics of beta cell mass in a mouse model of congenital hyperinsulinism: implications for type 2 diabetes.
- Glucokinase is required for high-starch diet-induced β-cell mass expansion in mice.
Tsuchida, Journal of diabetes investigation 2021 - GeneRIF: Glucokinase is required for high-starch diet-induced beta-cell mass expansion in mice.
- Ablation of glucokinase-expressing tanycytes impacts energy balance and increases adiposity in mice.
Rohrbach, Molecular metabolism 2021 - GeneRIF: Ablation of glucokinase-expressing tanycytes impacts energy balance and increases adiposity in mice.
- Berberine alleviates hyperglycemia by targeting hepatic glucokinase in diabetic db/db mice.
Li, Scientific reports 2019 - GeneRIF: Berberine alleviates hyperglycemia by targeting hepatic glucokinase in diabetic db/db mice.
- α-cell glucokinase suppresses glucose-regulated glucagon secretion.
Basco, Nature communications 2018 - GeneRIF: Glucokinase governs an alpha-cell metabolic pathway that suppresses secretion at or above normoglycemic levels; abnormal suppression of glucagon secretion deregulates hepatic glucose metabolism and, over time, induces a pre-diabetic phenotype.
- Octanoic acid potentiates glucose-stimulated insulin secretion and expression of glucokinase through the olfactory receptor in pancreatic β-cells.
Leem, Biochemical and biophysical research communications 2018 (PubMed)- GeneRIF: Long-term treatment with octanoic acid increased cellular glucose uptake in MIN6 cells by up-regulating the expression of glucokinase (GK).
- Impaired β-cell glucokinase as an underlying mechanism in diet-induced diabetes.
Lu, Disease models & mechanisms 2018 - GeneRIF: These data also imply an etiological role of GCK in diet-induced diabetes.
- More
HCAG_03661 hexokinase from Histoplasma mississippiense (nom. inval.)
23% identity, 49% coverage
Ccel_3221 hexokinase from Clostridium cellulolyticum H10
Ccel_3221 hexokinase family protein from Ruminiclostridium cellulolyticum H10
25% identity, 60% coverage
- Handling Several Sugars at a Time: a Case Study of Xyloglucan Utilization by Ruminiclostridium cellulolyticum
Kampik, mBio 2021 - “...included in the present study. Analysis of the genome suggested that the genes at loci Ccel_3221 and Ccel_3238 encode the sole hexokinase and galactokinase, respectively ( Fig.1b ). Regarding the catabolism of xylose, four genes (at loci Ccel_0500, Ccel_0941, Ccel_1925, and Ccel_3429) potentially encode a xylose...”
- “...of xylose, galactose or cellobiose at high concentrations. Inactivation of its encoding gene at locus Ccel_3221 was successfully conducted using the ClosTron technology ( 25 ) (see Fig.S3 ). The resulting mutant strains, MTL3221 and MTL3221(p0) carrying an empty expression vector, were expectedly unable to grow...”
- Structure and regulation of the cellulose degradome in Clostridium cellulolyticum
Xu, Biotechnology for biofuels 2013 - “...to late-log phase (Figure 4 D); iii) The NTA of putative glucokinase genes (Ccel_0700 and Ccel_3221, the first enzyme in the Embden-Meyerhof pathway) under glucose were 36~58% lower than under other soluble sugars such as xylose and cellobiose (Additional file 3 : Table S2); iv) Under...”
Q4VT69 Phosphotransferase (Fragment) from Leishmania infantum
22% identity, 86% coverage
E9BF15 Phosphotransferase from Leishmania donovani
A4HZB2 Phosphotransferase from Leishmania infantum
LINJ_21_0310 putative hexokinase from Leishmania infantum JPCM5
LDBPK_210300 hexokinase, putative from Leishmania donovani
22% identity, 86% coverage
- Comprehensive proteomic analysis of autophagosomes derived from Leishmania-infected macrophages
Nandan, PloS one 2023 - “...E9BE16 ADP/ATP translocase LDBPK_190710 E9BE66 Glycosomal Malate dehydrogenase LDBPK_201350 E9BET4 Calpain-like cysteine peptidase, putative LDBPK_210300 E9BF15 Hexokinase LDBPK_240140 E9BGM6 Glycosomal membrane like protein LDBPK_251210 E9BHM5 ATP synthase subunit beta LDBPK_261220 E9BID4 Heat shock protein 70-related protein LDBPK_072500 E9BJI0 Phosphoenolpyruvate carboxykinase (ATP) LDBPK_282430 E9BJW3 Glycosomal membrane protein,...”
- Potential selection of antimony and methotrexate cross-resistance in Leishmania infantum circulating strains
Bernardo, PLoS neglected tropical diseases 2024 - “...synthetase 47.87 45.39 2.48 A4I5D2 LINF 300015700 Ubiquitin conjugation factor E4 Bputative 48.48 46.13 2.35 A4HZB2 LINF 210007900 Hexokinaseputative 51.94 49.74 2.2 Q95NF5 LINF 150019000 Tryparedoxin peroxidase 49.76 47.58 2.18 A4I931 LINF 330024300 3-ketoacyl-CoA reductaseputative 44.72 42.56 2.16 E9AHH9 LINF 280015700 Putative ribosomal protein S20 45.77...”
- “...ADPATP carrier protein 1 43.94 43.78 0.16 A4HX65 LINF 170005000 Uncharacterized protein 43.19 43.04 0.15 A4HZB2 LINF 210007900 Hexokinaseputative 51.84 51.77 0.07 A4ICK8 LINF 360018400 Fructose bisphosphate aldolase 46.7 46.65 0.05 A4HW34 LINF 140015200 Glutathione synthetase 50.28 50.23 0.05 A4I7K4 LINF 320009100 Putative ATP-dependent RNA helicase...”
- Antileishmanial Activity of Cinnamic Acid Derivatives against Leishmania infantum
de, Molecules (Basel, Switzerland) 2023 - “...trans isomerase (PPIase) A4I004 CYP6 Peptidyl-prolyl cis - trans isomerase (PPIase) A4IC14 CYP40 Cyclophilin 40 A4HZB2 PPT Phosphotransferase A4I3K7 PAH Phenylalanine-4-hydroxylase molecules-28-02844-t004_Table 4 Table 4 Docking scores for the top-scored conformer. Target PLP (a) Z_PLP (b) GS (c) Z_GS (d) CS (e) Z_CS (f) ASP (g)...”
- Exploring direct and indirect targets of current antileishmanial drugs using a novel thermal proteomics profiling approach
Ibarra-Meneses, Frontiers in cellular and infection microbiology 2022 - “...(threonyl and isoleucyl), several ribosomal proteins (L24, L34 S2, S4, S15 and S18), a phosphotransferase (A4HZB2), a malic enzyme (A4I0R5), a fumarate hydratase (A2CIN2), and a citrate synthase (A4HXU5). On the other hand, the 5 significant proteins found in the MF200.5 melting proteome included a centromere/microtubule...”
- “...L24 49.7 40.4 9.3 No 0 A4I0R5 LINF_240012700 Malic enzyme 48.5 40.3 8.2 No 0 A4HZB2 LINF_210007900 Phosphotransferase 55.3 47.6 7.7 No 0 A4IDD6 LINF_360022900 Proteasome subunit beta type-5 59.9 52.8 7.1 No 0 A4ICP1 LINF_360015100 Putative 40S ribosomal protein S18 52.0 45.6 6.3 No 0...”
- Unravelling the proteomic signature of extracellular vesicles released by drug-resistant Leishmania infantum parasites
Douanne, PLoS neglected tropical diseases 2020 - “...(0.295) no 2 LINJ_27_1710 Glycosomal phosphoenol pyruvate carboxykinase, putative 66.3 no no (0.279) no 3 LINJ_21_0310 Phosphotransferase 52.0 no no (0.454) no 4 LINJ_11_1000 Pyruvate, phosphate dikinase 34.3 no no (0.442) no 5 LINJ_36_1320 Fructose-bisphosphate aldolase 32.0 no no (0.547) no 6 LINJ_35_1190 Putative NADH-dependent fumarate...”
- Comprehensive proteomic analysis of autophagosomes derived from Leishmania-infected macrophages
Nandan, PloS one 2023 - “...LDBPK_190200 E9BE16 ADP/ATP translocase LDBPK_190710 E9BE66 Glycosomal Malate dehydrogenase LDBPK_201350 E9BET4 Calpain-like cysteine peptidase, putative LDBPK_210300 E9BF15 Hexokinase LDBPK_240140 E9BGM6 Glycosomal membrane like protein LDBPK_251210 E9BHM5 ATP synthase subunit beta LDBPK_261220 E9BID4 Heat shock protein 70-related protein LDBPK_072500 E9BJI0 Phosphoenolpyruvate carboxykinase (ATP) LDBPK_282430 E9BJW3 Glycosomal membrane...”
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory