PaperBLAST
PaperBLAST Hits for sp|Q9K8V5|ARGD_HALH5 Acetylornithine aminotransferase OS=Halalkalibacterium halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=argD PE=3 SV=1 (384 a.a., MSHLFPTYAK...)
Show query sequence
>sp|Q9K8V5|ARGD_HALH5 Acetylornithine aminotransferase OS=Halalkalibacterium halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=argD PE=3 SV=1
MSHLFPTYAKWDVTITSGKGTKLYDNQGNEYLDFVSGIAVCNLGHCHPKVVAAVEEQLHS
FWHVSNLFHIPIQENVAALLTEQSGMDAVFFCNSGAEANEAAIKLARKATGKHEIVTFTQ
SFHGRTLGTMSATGQDKIKTGFGPMLETFHHVPFNDIAALKQVVNEQTAAIVLEVIQGEG
GVNLIDPEFAASVNHVCQEHGILLIIDEIQTGIGRTGTAFAFQQYELTPDIITVAKGLGN
GFPVGAMLGKQHLIDAFSAGSHGSTFGGNPLAMAAAQAVLTEVFQPNFLQAVQEKGKQLL
SGLNEALSGLEIVKEIRGNGLLVGIELQEEGAPFIKQLREKGLLVLNAGPNVIRLLPPLV
VTSEELHEAVTQLKEVLDQASVHA
Running BLASTp...
Found 250 similar proteins in the literature:
ATE50_RS13990 acetylornithine transaminase from Bacillus velezensis
60% identity, 99% coverage
GL331_10540 acetylornithine transaminase from Bacillus velezensis
60% identity, 99% coverage
GBAA4352 acetylornithine aminotransferase from Bacillus anthracis str. 'Ames Ancestor'
60% identity, 99% coverage
BSU11220 acetylornithine aminotransferase from Bacillus subtilis subsp. subtilis str. 168
P36839 Acetylornithine aminotransferase from Bacillus subtilis (strain 168)
60% identity, 98% coverage
- rocF affects the production of tetramethylpyrazine in fermented soybeans with Bacillus subtilis BJ3-2
Liu, BMC biotechnology 2022 - “...Up BSU02350 68.903 174.218 1.34 9.74E-04 3.42E-02 Up BSU31050 64.173 161.213 1.33 1.13E-03 3.79E-02 Up BSU11220 71.588 176.107 1.3 1.30E-03 4.18E-02 Up BSU15510 1928.76 14.424 7.05 1.22E-13 2.22E-10 Down BSU15520 2240.655 18.756 6.89 1.97E-13 2.40E-10 Down BSU15500 1042.241 8.719 6.88 5.66E-13 4.13E-10 Down BSU15530 2030.044 18.342...”
- A Survey of Pyridoxal 5'-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis
Richts, Frontiers in molecular biosciences 2019 - “...PDB no. b Function Pathway Regulation Paralogs/Protein familiy c References METABOLISM Amino acid biosynthesis ArgD BSU11220 No 2.6.1.11 2EH6 ( Aquifex aeolicus , 45%) Acetylornithine aminotransferase Arginine biosynthesis SigA, AhrC (), CodY (), YlxR () PLP AAT class III family AAT, ArgD subfamily Czaplewski et al.,...”
- Modular architecture of metabolic pathways revealed by conserved sequences of reactions
Muto, Journal of chemical information and modeling 2013 - “...PF0940 pyruvate 2-oxobutanoate M00535 (bth) BT_1858 BT_1860+BT_1859 BT_1857 RM002 2-aminoadipate lysine M00028 (bsu) BSU11200 BSU11210+BSU11190 BSU11220 glutamate ornithine M00031 (ttr) Tter_0315+Tter_0316 Tter_0320 Tter_0319 Tter_0321 Tter_0317 a KEGG organism codes are shown in parentheses: pfu (T00075), Pyrococcus furiosus DSM 3638; bth (T00122), Bacteroides thetaiotaomicrometer VPI-5482; bsu (T00010),...”
- Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis
Liu, Journal of bacteriology 2010 - “...B. subtilis 2, BSU13580; B. subtilis 3, BSU11220; B. subtilis 4, BSU03900; Bacteroides, BF2666; Bordetella 1, BPP2543; Bordetella 2, BPP1996; Synechocystis,...”
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...P77646, P05707, P37769, P0A9P9, P37760, P32055, P09147, P67910, P37759, P0AC88, P27830), Class III Aminotransferase family (P36839, P94427, P53555, P71084, P30949, P33189, O34662, P38021, P22256, P50457, P12995, P23893, P48247, P18335, P77581, O30508, Q9I606, Q9I6 M4, Q9I693, Q9I700, Q9I6J2, Q9I6R7, Q9HV04, Q9HTP1, Q9HWU0, Q9HT50, P48247, Q9I168, P12677, Q82...”
lmo1588 highly similar to N-acetylornithine aminotransferase from Listeria monocytogenes EGD-e
53% identity, 98% coverage
- Integrative genomic analysis identifies isoleucine and CodY as regulators of Listeria monocytogenes virulence
Lobel, PLoS genetics 2012 - “...rhaB 02420.6 (lmo2849) rhamnulokinase L-rhamnose utilization hisC 01072.6 (lmo1925) histidinol-phosphate aminotransferase Histidine biosynthesis argD 01379.6 (lmo1588) acetylornithine aminotransferase Arginine biosynthesis ilvC 01134.6 (lmo1986) ketol-acid reductoisomerase Branched chain amino acids biosynthesis Metabolic requirements influence the expression of major virulence genes Our analyses established that four anabolic pathways...”
KO21_RS08195 acetylornithine transaminase from Listeria monocytogenes
53% identity, 98% coverage
Q71Z79 Acetylornithine aminotransferase from Listeria monocytogenes serotype 4b (strain F2365)
53% identity, 98% coverage
UAI_00297 acetylornithine transaminase from Enterococcus malodoratus ATCC 43197
52% identity, 98% coverage
T303_03440 acetylornithine transaminase from Streptococcus thermophilus ASCC 1275
52% identity, 98% coverage
Teth39_0226 acetylornithine and succinylornithine aminotransferase from Thermoanaerobacter ethanolicus ATCC 33223
47% identity, 95% coverage
SGO_1566 Acetylornithine aminotransferase (ACOAT) from Streptococcus gordonii str. Challis substr. CH1
49% identity, 99% coverage
SSA_0760 Acetylornithine aminotransferase, putative from Streptococcus sanguinis SK36
48% identity, 99% coverage
- Glucose Phosphotransferase System Modulates Pyruvate Metabolism, Bacterial Fitness, and Microbial Ecology in Oral Streptococci
Zeng, Journal of bacteriology 2023 (secret) - Transcriptome, Phenotypic, and Virulence Analysis of Streptococcus sanguinis SK36 Wild Type and Its CcpA-Null Derivative (ΔCcpA)
Bai, Frontiers in cellular and infection microbiology 2019 - “...a 1,238.80 394.95 1.96 0.00000 c184_g210 SSA_0094 1,330.37 327.41 2.23 0.00000 c184_G204 b , c SSA_0760 74.75 6.52 3.50 0.00000 c184_g309 SSA_1052 1,412.90 243.16 2.55 0.00000 c184_G315 b , c , m SSA_0757 44.03 4.06 3.35 0.00000 c184_G202 b , c , m SSA_0758 56.88 5.36...”
- Systematic study of genes influencing cellular chain length in Streptococcus sanguinis
Evans, Microbiology (Reading, England) 2014 - “...shorter chains in their mutants with upregulated gene expressions in Ssx_1972. These genes were SSA_0759, SSA_0760, SSA_1715, SSA_1897, SSA_2117, SSA_2141, SSA_2159 and SSA_2337. All positively and negatively related genes should contribute to the cellular chain length of Ssx_1972. However, there were also downregulated genes showing shorter...”
- “...Y SSA_2077 Hypothetical protein SSA_2077 0.4932 0.0512 Y SSA_0759 argB Acetylglutamate kinase 3.1950 0.6376 Y SSA_0760 Acetylornithine aminotransferase 9.4869 4.9372 Y SSA_1715 serC Phosphoserine aminotransferase 2.0630 1.0319 Y SSA_1897 Hypothetical protein SSA_1897 2.2604 0.6261 Y SSA_2117 rmuC DNA recombination protein RmuC 2.5179 0.7197 Y SSA_2141 argH...”
GSU0151 acetylornithine aminotransferase from Geobacter sulfurreducens PCA
46% identity, 95% coverage
- Silica immobilization of Geobacter sulfurreducens for constructing ready-to-use artificial bioelectrodes
Estevez-Canales, Microbial biotechnology 2018 - “...biosynthetic process 3,92 FabG2 GSU1140 Methylaccepting sensory transducer class 34H. Signal transducer activity 3,83 mcp34H3 GSU0151 Acetylornithine aminotransferase. Arginine biosynthetic process 3,73 argD GSU0841 Sigma54dependenttranscriptional response regulator 3,68 GSU1607 Serine hydroxymethyltransferase. Glycine biosynthetic process 3,63 glyA GSU1602 MalonylCoA carrier protein transacylase. Fatty acid biosynthetic process 3,6...”
- “...include genes involved in the synthesis and metabolism of compatible solutes such as amino acids (GSU0151, GSU1607, GSU2371, GSU0153, GSU2874) and sugar alcohol (GSU3125), which were overexpressed between 4.8 and threefold. Another bacterial common response to environmental changes, including osmotic stress, is the modification of the...”
OKIT_0630 acetylornithine transaminase from Oenococcus kitaharae DSM 17330
48% identity, 99% coverage
CC0584 succinylornithine transaminase, putative from Caulobacter crescentus CB15
46% identity, 95% coverage
CA_C2388, CEA_G2403 aspartate aminotransferase family protein from Clostridium acetobutylicum ATCC 824
CAC2388 N-acetylornithine aminotransferase from Clostridium acetobutylicum ATCC 824
44% identity, 97% coverage
- Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach
Yoo, Biotechnology for biofuels 2016 (no snippet) - A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum
Yoo, mBio 2015 - “...to 40-fold) of genes involved in serine biosynthesis (CA_C0014-CA_C0015), seryl-tRNA synthesis (CA_C0017), and arginine biosynthesis (CA_C2388) was detected at the mRNA level and confirmed by the proteomic analysis, in agreement with a previous metabolomic study in batch culture ( 67 ), which reported higher intracellular concentrations...”
- “...synthesis and histidyl-tRNA synthesis (CA_C0935-CA_C0943) and 5-fold upregulation of a gene involved in arginine biosynthesis (CA_C2388) were also detected at the mRNA level and confirmed by the proteomic analysis. These genes were also shown to be upregulated under solventogenic conditions (this study) and in response to...”
- Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in Clostridium acetobutylicum EA 2018
Hu, BMC genomics 2011 - “...CA_C2377 -4.81354 -4.50847 -4.89131 -5.25303 -5.6598 -6.94396 oligopeptide ABC-type transporter, periplasmic binding component (frameshift) CEA_G2403 CA_C2388 -3.653 6.58659 9.490741 7.214547 3.839977 1.277684 acetylornithine aminotransferase CEA_G2405 CA_C2390 -4.029 7.044851 9.332151 8.593887 5.225523 1.729156 N-acetyl-gamma-glutamyl-phosphate reductase CEA_G2406 CA_C2391 -3.63924 6.659273 9.430206 8.041522 4.889782 1.658329 bifunctional ornithine acetyltransferase/N-acetylglutamate synthase...”
- “...CEA_G2392 CA_C2377 -4.81354 -4.50847 -4.89131 -5.25303 -5.6598 -6.94396 oligopeptide ABC-type transporter, periplasmic binding component (frameshift) CEA_G2403 CA_C2388 -3.653 6.58659 9.490741 7.214547 3.839977 1.277684 acetylornithine aminotransferase CEA_G2405 CA_C2390 -4.029 7.044851 9.332151 8.593887 5.225523 1.729156 N-acetyl-gamma-glutamyl-phosphate reductase CEA_G2406 CA_C2391 -3.63924 6.659273 9.430206 8.041522 4.889782 1.658329 bifunctional ornithine acetyltransferase/N-acetylglutamate...”
- Capturing the response of Clostridium acetobutylicum to chemical stressors using a regulated genome-scale metabolic model
Dash, Biotechnology for biofuels 2014 - “...CAC3020 Bifunctional ornithine acetyltransferase/N-acetylglutamate synthase (OACT/AGS) Med CAC2391 or CAC3020 Bifunctional ornithine acetyltransferase/N-acetylglutamate synthase High CAC2388 or CAC0368 4-aminobutyrate aminotransferase acetylornithine/N-succinyldiaminopimelate aminotransferase (GABAT/ARGD) If regulatory bounds were imposed exclusively on the reactions in the core set, the flux ranges would match those of the model with...”
- Meta-analysis and functional validation of nutritional requirements of solventogenic Clostridia growing under butanol stress conditions and coutilization of D-glucose and D-xylose
Heluane, Applied and environmental microbiology 2011 - “...I CAC2024 0.009199846 E E E E E CAC2388 CAC2389 CAC2390 CAC2723 CAC2805 2.72258E05 0.000250975 0.003281643 0.002038606 0.000640894 E E E CAC3164 CAC3348 CAC3618...”
- “...I E E E I I P P CAC2235 CAC2388 CAC2389 CAC2390 CAC2634 CAC2708 CAC2711 CAC2712 CAC3164 CAC3170 CAC3348 CAC3462 CAC3596 CAC3680 CAC3681 0.40506 1.51803 1.07553...”
- The role of PerR in O2-affected gene expression of Clostridium acetobutylicum
Hillmann, Journal of bacteriology 2009 - “...CAC3659 Arginine biosynthesis CAC0316 CAC0376 CAC0973 CAC0974 CAC2388 CAC2389 CAC2390 CAC2391 Protein function COG class O2 sufB1 sufB2 Fe-regulated ABC-type...”
- “...CAC3076, CAC3657 to CAC3659, CAC0973 and CAC0974, and CAC2388 to CAC2391 are genes that presumably form a transcriptional unit, respectively. b As identified...”
- Genome-scale model for Clostridium acetobutylicum: Part I. Metabolic network resolution and analysis
Senger, Biotechnology and bioengineering 2008 - “...BLASTP. Bit-scores suggested orthology ( Pearson, 1996 ) of both enzymes (of both organisms) with CAC2388. When further analyzed in the BRENDA enzyme database, multiple researchers have noted that acetylornithine transaminase (EC 2.6.1.11) may exhibit very similar enzymatic activity to ornithine-oxo-acid transferase (EC 2.6.1.13) ( Billheimer...”
- “...and Meyer, 1985 ), we propose the following biochemical reaction catalyzed by the enzyme of CAC2388, which is currently annotated as an acetylornithine transaminase (ArgD, EC 2.6.1.11) in C. acetobutylicum : (3) L ornithine + pyruvate ( ArgD , RocD , EC 2.6.11 , EC 2.6.1.13...”
- Genome-scale model for Clostridium acetobutylicum: Part II. Development of specific proton flux states and numerically determined sub-systems
Senger, Biotechnology and bioengineering 2008 - “...of pyruvate and L-ornithine to L-glutamate and L-alanine by the acetylornithine transaminase (ArgD, EC 2.6.1.11, CAC2388) and (ii) biosynthesis of L-glutamate through the L-arginine biosynthesis pathway in the presence of a large intracellular L-glutamate solute pool. Thus, L-alanine is a product of L-glutamate biosynthesis by ArgD....”
- “...was resolved by varying the ratio of Reaction 1 (through acetylornithine transaminase (ArgD, EC 2.6.1.11, CAC2388)) to Reaction 4 (L-glutamate biosynthesis through L-arginine biosynthesis pathway). Reaction 2 is catalyzed by the alanine racemace (EC 5.1.1.1, CAC0492) and D-alanine transaminase (EC 2.6.2.21, CAC0792) drives Reaction 3. Not...”
STH2881 N-acetylornithine aminotransferase from Symbiobacterium thermophilum IAM 14863
45% identity, 93% coverage
B1XNF8 acetylornithine transaminase (EC 2.6.1.11); 4-aminobutyrate-2-oxoglutarate transaminase (EC 2.6.1.19) from Synechococcus sp. PCC 7002 (see paper)
SYNPCC7002_A0326 acetylornithine aminotransferase from Synechococcus sp. PCC 7002
44% identity, 90% coverage
tlr1328 N-acetylornithine aminotransferase from Thermosynechococcus elongatus BP-1
46% identity, 90% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...Synechococcus Yellowstone sp. JA-2-3Ba (2-13) CYB_1744 CYB_1419, CYB_2128 CYB_0516, CYB_0715, CYB_1893 Thermosynechococcus elongatus BP-1 n.d. Tlr1328, Tlr0408, Tll1935 Tlr0416, Tlr0221 Synechocystis sp. PCC 6803 Sll1077, Sll0228 Slr1022 Sll1561, Slr0370, Slr0091 Gloeobacter violaceus PCC 7421 n.d. Glr0547, Glr3849, Gll2223 Glr2755, Glr3848, Gll1504, Gll2805 Nostoc sp. PCC 7120...”
- “...Synechococcus Yellowstone sp. JA-2-3Ba (2-13) CYB_0250 CYB_1419, CYB_2128 CYB_0516, CYB_0715, CYB_1893 Thermosynechococcus elongatus BP-1 Tll0507 Tlr1328, Tlr0408, Tll1935 Tlr0416, Tlr0221 Synechocystis sp. PCC 6803 Sll1336 Slr1022 Sll1561, Slr0370, Slr0091 Gloeobacter violaceus PCC 7421 Glr1758 Glr0547, Glr3849, Gll2223 Glr2755, Glr3848, Gll1504, Gll2805 Nostoc sp. PCC 7120 Alr4495...”
Atu0428 bifunctional N-succinyldiaminopimelate-aminotransferase/acetylornithine transaminase protein from Agrobacterium tumefaciens str. C58 (Cereon)
Q8UI71 Acetylornithine aminotransferase from Agrobacterium fabrum (strain C58 / ATCC 33970)
46% identity, 93% coverage
- Coordinated Regulation of Species-Specific Hydroxycinnamic Acid Degradation and Siderophore Biosynthesis Pathways in Agrobacterium fabrum
Baude, Applied and environmental microbiology 2016 - “...of 12 other genes (atu0043, atu0080, atu0413, atu0428, atu1226, atu1250, atu1765, atu1766, atu3140, atu3356, atu3778, and atu3997). These genes encode proteins...”
- Diverse protein regulations on PHA formation in Ralstonia eutropha on short chain organic acids
Lee, International journal of biological sciences 2009 - “...cystathionine gamma-lyase 2 29 (21) Q59829 Streptomyces coelicolor Cysteine biosynthesis Acetylornitine aminotransferase 2 21 (17) Q8UI71 Agrobacterium tumefaciens Arginine biosynthesis Pyrimidine biosynthesis Quinolinate synthetase A 2 24 (17) Q9F364 Streptomyces coelicolor NAD biosynthesis Bifunctional purine biosynthesis protein purH 7 33 (19) Q9JZM7 Neisseria meningitidis Nucleotide biosynthesis...”
2eh6A / O66442 Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
45% identity, 98% coverage
- Ligand: pyridoxal-5'-phosphate (2eh6A)
DDA3937_RS19450 aspartate aminotransferase family protein from Dickeya dadantii 3937
44% identity, 92% coverage
CwatDRAFT_5161 Acetylornithine and succinylornithine aminotransferase from Crocosphaera watsonii WH 8501
43% identity, 91% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 RS9917_06190 RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera watsonii WH 8501 n.d. CwatDRAFT_5161 CwatDRAFT_0865, CwatDRAFT_0842, CwatDRAFT_0969 Trichodesmium erythraeum IMS 101 TeryDRAFT_4567 TeryDRAFT_3251 TeryDRAFT_2672 TeryDRAFT_3296, TeryDRAFT_3923 Freshwater species Synechococcus elongatus sp. PCC 6301 n.d. Syc0599_c, Syc1466_c Syc1030_d Synechococcus elongatus sp. PCC 7942 n.d. Synpcc7942_0943,...”
- “...WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 n.d. RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera watsonii WH 8501 CwatDRAFT_0830 CwatDRAFT_5161 CwatDRAFT_0865, CwatDRAFT_0842, CwatDRAFT_0969 Trichodesmium erythraeum IMS 101 TeryDRAFT_2282 TeryDRAFT_3251 TeryDRAFT_2672 TeryDRAFT_3296, TeryDRAFT_3923 Freshwater species Synechococcus elongatus sp. PCC 6301 n.d. Syc0599_c, Syc1466_c Syc1030_d Synechococcus elongatus sp. PCC 7942 n.d. Synpcc7942_0943,...”
slr1022 / P73133 bifunctional acetylornithine transaminase/4-aminobutyrate—2-oxoglutarate transaminase (EC 2.6.1.19; EC 2.6.1.11) from Synechocystis sp. (strain PCC 6803 / Kazusa) (see 4 papers)
ARGD_SYNY3 / P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see 2 papers)
P73133 acetylornithine transaminase (EC 2.6.1.11); 4-aminobutyrate-2-oxoglutarate transaminase (EC 2.6.1.19) from Synechococcus sp. PCC 6803 (see paper)
slr1022 N-acetylornithine aminotransferase from Synechocystis sp. PCC 6803
45% identity, 88% coverage
- function: Catalyzes the reversible conversion of N-acetylornithine to N-acetylglutamate-5-semialdehyde (PubMed:36982927). In vitro, also shows very low ornithine aminotransferase (OAT) and gamma-aminobutyrate aminotransferase (GABA-AT) activity, catalyzing the conversion of ornithine (Orn) to glutamate-5-semialdehyde and of gamma-aminobutyric acid (GABA) to succinate semialdehyde (PubMed:36982927). It has been shown to function as a GABA-AT and contributes to closing the tricarboxylic acid cycle of Synechocystis sp. PCC6803 via the GABA shunt (PubMed:24989231). However, the catalytic efficiency toward N- acetylornithine is 2500-fold and 10700-fold higher than that toward ornithine and gamma-aminobutyrate, respectively, indicating that the protein mainly functions as an N-acetylornithine aminotransferase (PubMed:36982927).
catalytic activity: N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L- glutamate 5-semialdehyde + L-glutamate (RHEA:18049)
cofactor: pyridoxal 5'-phosphate (Binds 1 pyridoxal phosphate per subunit.)
subunit: Homodimer.
disruption phenotype: Mutant lacking this gene cannot grow in BG11 medium without the addition of citrulline or arginine (PubMed:24989231). The level of succinate in the mutant is reduced to 30-35% of that in the wild-type strain (PubMed:24989231). - Arginine inhibits the arginine biosynthesis rate-limiting enzyme and leads to the accumulation of intracellular aspartate in Synechocystis sp. PCC 6803
Katayama, Plant molecular biology 2024 - “...the GT strain, respectively (Fig. 2 c). The expression levels of the gene encoding argD (slr1022) and aspC (sll0402) were not significantly different among the GT, ArgGOX, and ArgHOX strains (Fig. 2 c). We also measured RNA expression of the argB gene encoding NAGK that is...”
- “...Liu J Li Z Kinetic characterization and catalytic mechanism of N -acetylornithine aminotransferase encoded by slr1022 gene from Synechocystis sp. PCC 6803 Int J Mol Sci 2023 24 5853 10.3390/ijms24065853 36982927 Liu D Yang C The nitrogen-regulated response regulator NrrA controls cyanophycin synthesis and glycogen catabolism...”
- Kinetic Characterization and Catalytic Mechanism of N-Acetylornithine Aminotransferase Encoded by slr1022 Gene from Synechocystis sp. PCC6803
Li, International journal of molecular sciences 2023 - “...36982927 10.3390/ijms24065853 ijms-24-05853 Article Kinetic Characterization and Catalytic Mechanism of N -Acetylornithine Aminotransferase Encoded by slr1022 Gene from Synechocystis sp. PCC6803 Li Zhi-Min 1 Bai Fumei 2 Wang Xiaoqin 2 Xie Congcong 2 Wan Yuting 2 Li Yating 2 Liu Jianping 2 3 * https://orcid.org/0000-0001-7036-9282 Li...”
- “...of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/ ). The enzyme encoded by slr1022 gene from Synechocystis sp. PCC6803 was reported to function as N -acetylornithine aminotransferase, -aminobutyric acid aminotransferase, and ornithine aminotransferase, which played important roles in multiple metabolic pathways. Among these functions,...”
- Chlorophyll biosynthesis under the control of arginine metabolism
Kiss, Cell reports 2023 - “...of the Synechocystis model strains The argD and cphB strains were constructed by replacing the slr1022 and slr2001 genes by erythromycin or spectinomycin resistance cassettes, respectively. The primers in the megaprimer PCR method used for mutagenesis are listed in TableS5 . The segregation of argD locus...”
- “...Y. Liu J. Li Z. Kinetic characterization and catalytic mechanism of N-acetylornithine aminotransferase encoded by slr1022 gene from Synechocystis sp. PCC 6803 Int. J. Mol. Sci. 24 2023 5853 10.3390/ijms24065853 36982927 33 Baers L.L. Breckels L.M. Mills L.A. Gatto L. Deery M.J. Stevens T.J. Howe C.J....”
- Phycobilisome breakdown effector NblD is required to maintain the cellular amino acid composition during nitrogen starvation
Krauspe, Journal of bacteriology 2021 (secret) - Improved sugar-free succinate production by Synechocystis sp. PCC 6803 following identification of the limiting steps in glycogen catabolism
Hasunuma, Metabolic engineering communications 2016 - “...2.48 2.52 2.28 NAD(P)H-quinone oxidoreductase subunit H sll0080 argC 2.47 2.48 2.61 N -acetyl-gamma-glutamyl-phosphate reductase slr1022 argD 2.45 2.78 3.44 acetylornithine aminotransferase ssr3383 apcC 2.44 2.45 1.66 phycobilisome LC linker polypeptide sll0920 ppc 2.38 2.87 2.47 phosphoenolpyruvate carboxylase slr0665 acnB 2.33 2.77 2.16 bifunctional aconitate hydratase...”
- Natural and Synthetic Variants of the Tricarboxylic Acid Cycle in Cyanobacteria: Introduction of the GABA Shunt into Synechococcus sp. PCC 7002
Zhang, Frontiers in microbiology 2016 - “...Purification, and Identification Open reading frames sll1641 ( gadA ), encoding the glutamate decarboxylase, and slr1022 ( argD ), encoding the N- acetylornithine aminotransferase of Synechocystis 6803, as well as open reading frame SYNPCC7002_A0326 ( argD ), encoding the N- acetylornithine aminotransferase of Synechococcus 7002, were...”
- The proteome and lipidome of Synechocystis sp. PCC 6803 cells grown under light-activated heterotrophic conditions
Plohnke, Molecular & cellular proteomics : MCP 2015 - “...shunt were detected, and the N-acetylornithine aminotransferase (Slr1022) was significantly increased in LAHG cultures. Increased abundance of proteins involved...”
- The γ-aminobutyric acid shunt contributes to closing the tricarboxylic acid cycle in Synechocystis sp. PCC 6803
Xiong, Molecular microbiology 2014 (PubMed)- “...series of mutant strains, sll1981, slr0370, slr1022 and combinations thereof, deficient in 2-oxoglutarate decarboxylase (Sll1981), succinate semialdehyde...”
- “...(Slr0370), and/or in -aminobutyrate metabolism (Slr1022) were constructed. Like in Pseudomonas aeruginosa, N-acetylornithine aminotransferase, encoded by...”
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alr1080 N-acetylornithine aminotransferase from Nostoc sp. PCC 7120
45% identity, 88% coverage
- The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box
Oliveira, Life (Basel, Switzerland) 2015 - “...Alr1050 Glucose-6-phosphate isomerase N 2 , NO 3 , NH 4 + Energy metabolism Anabaena Alr1080 Acetylornithine aminotransferase N 2 Amino acid biosynthesis - Alr1299 Phosphoribosylglycinamide formyltransferase 2 N 2 Purines, pyrimidines, nucleosides, and nucleotides - Alr1310 Alr1310 protein NH 4 + Conserved hypothetical protein -...”
- “...and processing (All2533, Alr0237, Alr0880, Alr0996, Alr1381, All4287, Alr1742, Alr4853, All2315, Alr1004, Alr1313, Alr2771, Alr0608, Alr1080, Alr2328 Alr4907, All1683, All4464), sugar breakdown and processing (Alr3608, Alr4448, All3964, All0167, All0168, All0875, Alr0169, Alr2190, All4539) and phosphor scavenge and transport (All0207, All2843, All4575, Alr4238, Alr4976). Detection of these...”
- Inactivation of agmatinase expressed in vegetative cells alters arginine catabolism and prevents diazotrophic growth in the heterocyst-forming cyanobacterium Anabaena
Burnat, MicrobiologyOpen 2014 - “...decarboxylase ( all3401 ); 2, agmatinase ( alr2310 ); 3, arginase; 4, ornithine transaminase ( alr1080 ); 5, 1 pyrroline-5-carboxylate reductase ( alr0488 ); 6, proline oxidase ( alr0540 ); 7, ornithine carbamoyltransferase ( alr4907 ); 8, arginine deiminase; 9, argininosuccinate synthetase ( alr4798 ); 10,...”
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...7421 n.d. Glr0547, Glr3849, Gll2223 Glr2755, Glr3848, Gll1504, Gll2805 Nostoc sp. PCC 7120 Alr2310 Alr2398, Alr1080, All0396 Alr0540, Alr3771, All3556, All5022 Nostoc punctiforme PCC 73102 Npun02002114 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena variabilis ATCC 29413 Ava_0127 Ava_0214, Ava_3730, Ava_2839 Ava_2942, Ava_1554, Ava_3534, Ava_2258 N.d....”
- “...7421 Glr1758 Glr0547, Glr3849, Gll2223 Glr2755, Glr3848, Gll1504, Gll2805 Nostoc sp. PCC 7120 Alr4495 Alr2398, Alr1080, All0396 Alr0540, Alr3771, All3556, All5022 Nostoc punctiforme PCC 73102 Npun02001803 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena variabilis ATCC 29413 Ava_2273 Ava_0214, Ava_3730, Ava_2839 Ava_2942, Ava_1554, Ava_3534, Ava_2258 N.d....”
AZOBR_RS19025 acetylornithine/N-succinyldiaminopimelate aminotransferase [EC:2.6.1.11 2.6.1.17] from Azospirillum brasilense Sp245
45% identity, 96% coverage
- mutant phenotype: Annotated as this by KEGG. Bifunctional argD. SEED has only the acetylornithine activity. (essential)
AZOBR_RS19025 aspartate aminotransferase family protein from Azospirillum baldaniorum
45% identity, 96% coverage
BAU10_13850 aspartate aminotransferase family protein from Vibrio alginolyticus
45% identity, 93% coverage
C6AZH0 Acetylornithine aminotransferase from Rhizobium leguminosarum bv. trifolii (strain WSM1325)
45% identity, 93% coverage
Ava_3730 Acetylornithine and succinylornithine aminotransferase from Anabaena variabilis ATCC 29413
45% identity, 88% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...73102 Npun02002114 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena variabilis ATCC 29413 Ava_0127 Ava_0214, Ava_3730, Ava_2839 Ava_2942, Ava_1554, Ava_3534, Ava_2258 N.d. = not detected. Table 7 Database entries of genes from 24 cyanobacterial genomes encoding putative L-arginine amidinotransferases (C1), L-ornithine transaminases (C2), and 1 pyrroline-5-carboxylate...”
- “...73102 Npun02001803 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena variabilis ATCC 29413 Ava_2273 Ava_0214, Ava_3730, Ava_2839 Ava_2942, Ava_1554, Ava_3534, Ava_2258 N.d. = not detected. Table 8 Database entries of genes from 24 cyanobacterial genomes encoding putative L-arginine deiminases (D1), L-ornithine transcarbamoylases (D2), carbamate kinases (D3),...”
Sant_0398 aspartate aminotransferase family protein from Sodalis praecaptivus
43% identity, 93% coverage
ECA4065 aspartate aminotransferase family protein from Pectobacterium atrosepticum SCRI1043
43% identity, 92% coverage
- Global Gene Expression Analysis of Cross-Protected Phenotype of Pectobacterium atrosepticum
Gorshkov, PloS one 2017 - “...under starvation genes belonging to different pathways were chosen for the verification: up-regulatedECA0999, ECA3238, ECA4082, ECA4065, ECA0049, ECA1065, ECA1216, ECA3920, ECA4477, ECA1059, ECA1166, ECA1540, ECA2013, ECA2873, ECA2226; down-regulatedECA3054, ECA3682, ECA4006, ECA4513, ECA1141, ECA1232, ECA4461, ECA1407, ECA2651, ECA4079, ECA0260, ECA0255, ECA0238, ECA3343, ECA3814. Total RNA was extracted...”
Cthe_1866 acetylornithine aminotransferase from Clostridium thermocellum ATCC 27405
45% identity, 92% coverage
- Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain
Linville, BMC microbiology 2014 - “...PM has two mutations involved with glutamate catabolism; a possible gain in function in argD (Cthe_1866, E55G) and a possible loss in function in proB (Cthe_1766, A149T) [ 17 ]. These two mutations seem to be a beneficial shift from proline production to glutamate and arginine...”
- Industrial robustness: understanding the mechanism of tolerance for the Populus hydrolysate-tolerant mutant strain of Clostridium thermocellum
Linville, PloS one 2013 - “...Cthe_0948 (1, CDS ) , Cthe_0949 (1, CDS ) , Cthe_1766 (1, CDS ) , Cthe_1866 (1, CDS ), Cthe_2529 (1, CDS) Production of various amino acids 2 5 Cthe_2602 (2, NC ) * , Cthe_2603 (2, CDS), Cthe_2607 (1, CDS) Single transcription unit Cthe_2602-9: Subunits...”
- “...mutations involved with glutamate catabolism. A mutation to a gene in the arginine pathway, argD (Cthe_1866 E55G) resulted in a pfam score of +1180. This common mutation has been previously observed in the C. thermocellum DSM 1313 strain [ 3 ]. Another mutation to a gene...”
ACICU_01135 ornithine/acetylornithine aminotransferase from Acinetobacter baumannii ACICU
45% identity, 91% coverage
VpaChn25_2721 aspartate aminotransferase family protein from Vibrio parahaemolyticus
44% identity, 93% coverage
- Genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (VpaCspA and VpaCspD) in Vibrio parahaemolyticus CHN25 during low-temperature survival
Zhu, BMC genomics 2017 - “...Hypothetical protein VpaChn25_2581 3.3917 Arginine deiminase VpaChn25_2719 2.8035 Succinylglutamic semialdehyde dehydrogenase VpaChn25_2720 3.9099 Arginine/ornithine succinyltransferase VpaChn25_2721 2.1486 Bifunctional N-succinyldiaminopimelate-aminotransferase/acetylornithine transaminase protein Valine, leucine and isoleucine degradation VpaChn25A_0480 3.4643 Acetyl-CoA acetyltransferase VpaChn25A_0555 2.023 Acyl-CoA carboxylase alpha chain VpaChn25A_0557 2.0591 Acyl-CoA carboxyltransferase beta chain VpaChn25A_0561 4.081 Aldehyde dehydrogenase...”
- “...cyclodeaminase VpaChn25_1544 0.3291 NAD-glutamate dehydrogenase VpaChn25_2719 0.3969 Succinylglutamic semialdehyde dehydrogenase VpaChn25_2720 0.482 Arginine /ornithine succinyltransferase VpaChn25_2721 0.2687 Bifunctional N-succinyldiaminopimelate-aminotransferase / acetylornithine transaminase protein Alanine, aspartate and glutamate metabolism VpaChn25_0345 0.2743 Glucosamine-fructose-6-phosphate aminotransferase VpaChn25_0438 2.461 Glutamate synthase subunit beta VpaChn25_0439 2.6963 Glutamate synthase, large subunit VpaChn25_1114 0.407...”
4jevB / P40732 N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
44% identity, 93% coverage
- Ligand: 3-[o-phosphonopyridoxyl]--amino-benzoic acid (4jevB)
ARGD_SALTY / P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
STM3468 acetylornithine transaminase (NAcOATase and DapATase) from Salmonella typhimurium LT2
44% identity, 92% coverage
- function: Involved in both the arginine and lysine biosynthetic pathways.
catalytic activity: N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L- glutamate 5-semialdehyde + L-glutamate (RHEA:18049)
catalytic activity: N-succinyl-(2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2-succinylamino-6-oxoheptanedioate + L-glutamate (RHEA:11960)
cofactor: pyridoxal 5'-phosphate (Binds 1 pyridoxal phosphate per subunit.)
subunit: Homodimer. - Intraspecies variation in the emergence of hyperinfectious bacterial strains in nature
Heithoff, PLoS pathogens 2012 - “...2.71 3.50 N-acetylglutamate synthase [91] . STM4122 argB 0.56 1.64 2.74 Acetylglutamate kinase [91] . STM3468 argD 1.26 2.84 4.31 Bifunctional N-succinyldiaminopimelate-aminotransferase/acetylornithine transaminase protein [91] . STM3290 argG 0.17 1.54 1.24 Arginosuccinate synthase [91] . STM4123 argH 0.20 1.53 2.10 Arginosuccinate lyase [91] . STM4469 argI...”
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...M4, Q9I693, Q9I700, Q9I6J2, Q9I6R7, Q9HV04, Q9HTP1, Q9HWU0, Q9HT50, P48247, Q9I168, P12677, Q82 MM1, P21267, P40732, Q8ZPV2, Q8ZLX7), Thiamine Diphosphate Decarboxylase family (P96591, P37251, P23970, Q04789, P42415, P07003, P0AEP7, P08142, P00892, P00893, P17109, P0AFI0, Q9HTQ7, Q9I3L0, Q9HVA0, Q9I3S7, Q9HUI8, Q9HUR2, Q9I280, Q9I207, Q9HYA3, Q9HWK1, Q8ZQF0, Q8ZNE8,...”
BAB1_0331 Acetylornithine and succinylornithine aminotransferase:Aminotransferase class-III from Brucella melitensis biovar Abortus 2308
BMEI1621 aspartate aminotransferase family protein from Brucella melitensis bv. 1 str. 16M
44% identity, 94% coverage
AWY96_RS23480 aspartate aminotransferase family protein from Serratia plymuthica
43% identity, 93% coverage
SEN3295 acetylornithine aminotransferase from Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
44% identity, 92% coverage
BPSL2390 succinylornithine transaminase from Burkholderia pseudomallei K96243
42% identity, 93% coverage
STM14_4175 aspartate aminotransferase family protein from Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
44% identity, 92% coverage
W6QU95 Acetylornithine aminotransferase from Pseudomonas pseudoalcaligenes (strain CECT 5344)
44% identity, 93% coverage
PBPRA0289 putative acetylornithine aminotransferase from Photobacterium profundum SS9
42% identity, 92% coverage
TTHA1755 acetylornithine/acetyl-lysine aminotransferase from Thermus thermophilus HB8
43% identity, 94% coverage
- Lysine propionylation is a prevalent post-translational modification in Thermus thermophilus
Okanishi, Molecular & cellular proteomics : MCP 2014 - “...TTHA1248 TTHA1262 TTHA0906 TTHA0980 TTHA1524 TTHA1698 TTHA1755 TTHA1781 TTHA1928 Carbohydrate transport and metabolism TTHA0002 TTHA0003 TTHA0106 TTHA0108...”
- Evolution of lysine biosynthesis in the phylum deinococcus-thermus
Nishida, International journal of evolutionary biology 2012 - “...TTC0043 TTC1012 TTC1393 TTC1396 TTC1541* TTC1542* TTC1543* TTC1546* TTC1547* TTC1550* Thermus thermophilus HB8 TTHA0411 TTHA1378 TTHA1755 TTHA1757 TTHA1903* TTHA1904* TTHA1907* TTHA1910* TTHA1911* TTHA1914* Thermus scotoductus TSC_c05810 TSC_c20650 TSC_c03550 TSC_c3520 TSC_c01940* TSC_c01930* TSC_c01920* TSC_c01890* TSC_c01880* TSC_c01850* Meiothermus ruber Mrub_0871 Mrub_2738 Mrub_0027 Mrub_2721* Mrub_2723* Mrub_2724* Mrub_2727* Mrub_2728* Meiothermus...”
Synpcc7942_0943 acetylornithine and succinylornithine aminotransferases from Synechococcus elongatus PCC 7942
44% identity, 88% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...Synechococcus elongatus sp. PCC 6301 n.d. Syc0599_c, Syc1466_c Syc1030_d Synechococcus elongatus sp. PCC 7942 n.d. Synpcc7942_0943, Synpcc7942_0031 Synpcc7942_0489 Synechococcus Yellowstone sp. JA-3-3-AB CYA_0859 CYA_1537, CYA_0689 CYA_0364 Synechococcus Yellowstone sp. JA-2-3Ba (2-13) CYB_1744 CYB_1419, CYB_2128 CYB_0516, CYB_0715, CYB_1893 Thermosynechococcus elongatus BP-1 n.d. Tlr1328, Tlr0408, Tll1935 Tlr0416, Tlr0221...”
- “...Synechococcus elongatus sp. PCC 6301 n.d. Syc0599_c, Syc1466_c Syc1030_d Synechococcus elongatus sp. PCC 7942 n.d. Synpcc7942_0943, Synpcc7942_0031 Synpcc7942_0489 Synechococcus Yellowstone sp. JA-3-3-AB n.d. CYA_1537, CYA_0689 CYA_0364 Synechococcus Yellowstone sp. JA-2-3Ba (2-13) CYB_0250 CYB_1419, CYB_2128 CYB_0516, CYB_0715, CYB_1893 Thermosynechococcus elongatus BP-1 Tll0507 Tlr1328, Tlr0408, Tll1935 Tlr0416, Tlr0221...”
BCAL1059 succinylornithine transaminase from Burkholderia cenocepacia J2315
42% identity, 95% coverage
- DNA Methylation Epigenetically Regulates Gene Expression in Burkholderia cenocepacia and Controls Biofilm Formation, Cell Aggregation, and Motility
Vandenbussche, mSphere 2020 - “...0.014 DnaA chromosomal replication initiation protein BCAL0509 1.129 0.473 1.175 0.199 MetK S -adenosylmethionine synthetase BCAL1059 1.129 0.662 0.767 0.457 ArgD bifunctional N -succinyldiaminopimelate-aminotransferase/acetylornithine transaminase protein BCAL1457 1.343 0.309 1.793 0.056 LysR family regulatory protein BCAL1515 1.790 0.032 1.869 0.012 SucA 2-oxoglutarate dehydrogenase E1 component BCAL2465...”
- The Small RNA ncS35 Regulates Growth in Burkholderia cenocepacia J2315
Kiekens, mSphere 2018 - “...2,3-dioxygenase Amino acid transport and metabolism BCAL1055 2.6 2.1 1.7 Histidine transport system permease protein BCAL1059 2.3 Succinylornithine transaminase BCAL1060 2.6 Arginine N -succinyltransferase BCAL1064 2.3 Succinylglutamate desuccinylase BCAL2933 4.0 4.0 2.4 d -Amino acid dehydrogenase Carbohydrate transport and metabolism BCAL1548 2.2 3.3 2.2 Sugar ABC...”
- Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315
Sass, BMC genomics 2015 - “...999245 ATG ATG shorter 1041114 - BCAL0952 Complement (1040227..1040955) 1041075 ATG ATG longer 1147555 + BCAL1059 1147734..1148927 1147695 ATG ATG longer 1161150 - BCAL1069 Complement (1159161..1160903) 1160996 ATG ATG longer 1207883 - BCAL1102 Complement (1207594..1207914) 1207848 GTG ATG shorter 1390938 - BCAL1277 Complement (1388805..1390997) 1390868 ATG...”
- The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111
Schmid, PloS one 2012 - “...protein 4.6 1.4 2.7 0.9 CCE53285 BCAL0831 Phasin family protein 2.9 0.7 3.2 1.4 CCE51557 BCAL1059 Bifunctional N-succinyldiaminopimelate-aminotransferase/acetylornithine transaminase protein 2.8 1.6 0.5 0.8 CCE50475 BCAL2352 Carbonic anhydrase 4.3 1.0 7.0 1.1 CCE49840 BCAL3041 Protein involved in carbohydrate transport 4.5 2.0 0.5 1.6 CCE51244 BCAL3285 Nitric...”
- Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment
O'Grady, Frontiers in cellular and infection microbiology 2011 - “...protein 1.81 BCAL1057 Histidine ABC transporter ATP-binding protein 1.98 BCAL1058 AraC family regulatory protein 2.22 BCAL1059 Succinylornithine transaminase 1.81 BCAL1060 Putative arginine N -succinyltransferase, alpha 1.63 BCAL1061 Putative arginine N -succinyltransferase, beta 1.86 BCAL1062 Succinylglutamic semialdehyde dehydrogenase 1.90 BCAL1063 Succinylarginine dihydrolase 2.58 BCAL1064 Putative succinylglutamate desuccinylase...”
- Use of suppression-subtractive hybridization to identify genes in the Burkholderia cepacia complex that are unique to Burkholderia cenocepacia
Bernier, Journal of bacteriology 2005 - “...BCAM2097 BCAM0648 BCAM0647 BCAL2851 BCAM0276 BCAL1598 BCAL1021 BCAL1059 BCAL1159 BCAL1185 BCAL0060 BCAM2312 BCAL1787 BCAL1783 1 1 1 1 Plasmid Plasmid Plasmid...”
YPTB1959 succinylornithine aminotransferase from Yersinia pseudotuberculosis IP 32953
44% identity, 91% coverage
ECs4210 acetylornithine delta-aminotransferase from Escherichia coli O157:H7 str. Sakai
Z4720 acetylornithine delta-aminotransferase from Escherichia coli O157:H7 EDL933
43% identity, 91% coverage
- Global transcriptional response of Escherichia coli O157:H7 to growth transitions in glucose minimal medium
Bergholz, BMC microbiology 2007 - “...- 3.24 2 ECs4758 metR -3.26 1 ECs4191 fusA -3.27 1 ECs4760 - 3.29 3 ECs4210 argD -2.36 1 ECs4774 fadB 6.58 2 ECs4212 fic 4.44 2 ECs4783 dsbA -2.15 1 ECs4213 yhfG 4.13 2 ECs4785 yihG 2.17 2 ECs4232 aroK -3.21 1 ECs4792 glnA -5.34...”
- Clonal and antigenic analysis of serogroup A Neisseria meningitidis with particular reference to epidemiological features of epidemic meningitis in the People's Republic of China
Wang, Infection and immunity 1992 - “...Z4313, Z4314, Z4315, Z4316, Z4317 I, IIa Z4713, Z4715, Z4716, Z4717, Z4718, Z4719, Z4720, Z4721, Z4722 I, IIa B375 29 30 31 32 33 34 35 J III 46 47 48 D D D D C...”
BPP2543 acetylornithine aminotransferase from Bordetella parapertussis 12822
44% identity, 96% coverage
UTI89_C3862 acetylornithine aminotransferase from Escherichia coli UTI89
43% identity, 91% coverage
Dtu / b3359 N-acetylornithine aminotransferase / N-succinyldiaminopimelate aminotransferase (EC 2.6.1.11; EC 2.6.1.17) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
argD / P18335 N-acetylornithine aminotransferase / N-succinyldiaminopimelate aminotransferase (EC 2.6.1.11; EC 2.6.1.17) from Escherichia coli (strain K12) (see 12 papers)
ARGD_ECOLI / P18335 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Escherichia coli (strain K12) (see paper)
b3359 bifunctional acetylornithine aminotransferase/ succinyldiaminopimelate aminotransferase from Escherichia coli str. K-12 substr. MG1655
NP_417818 N-acetylornithine aminotransferase/N-succinyldiaminopimelate aminotransferase from Escherichia coli str. K-12 substr. MG1655
43% identity, 91% coverage
- function: Involved in both the arginine and lysine biosynthetic pathways.
catalytic activity: N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L- glutamate 5-semialdehyde + L-glutamate (RHEA:18049)
catalytic activity: N-succinyl-(2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2-succinylamino-6-oxoheptanedioate + L-glutamate (RHEA:11960)
cofactor: pyridoxal 5'-phosphate (Binds 1 pyridoxal phosphate per subunit.)
subunit: Homodimer. - Improved Metabolic Models for E. coli and Mycoplasma genitalium from GlobalFit, an Algorithm That Simultaneously Matches Growth and Non-Growth Data Sets
Hartleb, PLoS computational biology 2016 - “...-77 b1702 PPS b2383 2x10 -22 2x10 -22 b3176 PGAMT b2048 3x10 -16 1x10 -18 b3359 SDPTA b1748 1x10 -180 1x10 -180 Solving FNp: Removing biomass components Removing metabolites from the biomass reaction can convert FNp to TPp, as all genes involved in the production (or,...”
- Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium
Cortés-Tolalpa, Microbial cell factories 2014 - “...N-acetylglutamate kinase Amino acid metabolism 45.8143 argC b3958 N-acetylglutamylphosphate reductase Amino acid metabolism 53.4932 argD b3359 N-succinyldiaminopimelate-aminotransferase/acetylornithine transaminase Amino acid metabolism 21.9053 argE b3957 Acetylornithine deacetylase Amino acid metabolism 18.7485 argG b3172 Argininosuccinate synthase Amino acid metabolism 65.2192 argH b3960 Argininosuccinate lyase Amino acid metabolism 23.7859...”
- Quantitative assessment of thermodynamic constraints on the solution space of genome-scale metabolic models
Hamilton, Biophysical journal 2013 - “...serA Nonlethal Lethal Nonlethal Nonlethal Nonlethal Nonlethal b3359 argD Nonlethal Lethal Nonlethal Lethal Nonlethal Lethal b3429 glgA Nonlethal Lethal...”
- Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions
Orth, BMC systems biology 2012 - “...none identified folP (b3177) none identified yrbG (b3196) none identified kdsC (b3198) none identified argD (b3359) astC (b1748) 1.00E-146 gabT (b2662) 3.00E-64 puuE (b1302) 3.00E-54 patA (b3073) 4.00E-52 hemL (b0154) 3.00E-32 cysG (b3368) none identified ilvE (b3770) pabC (b1096) 8.00E-8 dapF (b3809) none identified argC (b3958)...”
- “...This indicates that uncharacterized isozymes are possible for aspC (b0928), pabC (b1096), aldA (b1415), argD (b3359), and hemE (b3397). Another solution suggested that false negatives for aspC strains could be corrected by adding the existing model reaction ASP1DC (aspartate 1-decarboxylase) in reverse. No literature evidence was...”
- Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis
Liu, Journal of bacteriology 2010 - “...Chlamydia, CT390; E. coli 1, b2379; E. coli 2, b3359; Gloeobacter, glr4108; B. subtilis 1, BSU37690; B. subtilis 2, BSU13580; B. subtilis 3, BSU11220; B....”
- Genome-scale analysis to the impact of gene deletion on the metabolism of E. coli: constraint-based simulation approach
Xu, BMC bioinformatics 2009 - “...b0524 b2478 b0414, b0415, b0417 b3771 b0091 b0914 b2838 b0420, b0421, b0423 b3774 b0954 b0915 b3359 b0475, b0750, b0907 b1093 b0918 b3433 b1096, b1208, b1210 b1094 b1094 b3809 b1277, b1662, b1740 b1288 b1215 s0001 b1812, b2103, b2153 b2323 b3198 b2315, b2320, b2400 b3176 b3633 b2515, b2530,...”
- Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12
White-Ziegler, Journal of bacteriology 2007 - “...transport and metabolism argD b3359 Acetylornithine delta-aminotransferase aroF b2601 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase (DAHP synthetase),...”
- Experimental and computational assessment of conditionally essential genes in Escherichia coli
Joyce, Journal of bacteriology 2006 - “...(b3927) cysW (b2423) argB (b3959); argC (b3958); argD (b3359); argG (b3172) aspC (b0928) ilvY (b3773); ilvE (b3770); lrp (b0889) aldA (b1415) coaA (b3974); coaE...”
- More
- Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Link, Electrophoresis 1997 (PubMed)- GeneRIF: N-terminus verified by Edman degradation on mature peptide
- Knowns and Unknowns of Vitamin B6 Metabolism in Escherichia coli
Tramonti, EcoSal Plus 2021 (secret) - Prediction of Metabolic Pathway Involvement in Prokaryotic UniProtKB Data by Association Rule Mining
Boudellioua, PloS one 2016 - “...Methanosarcinaceae, TAXON: Methanosarcina, IPR: IPR017896, IPR:IPR017900, IPR: IPR004460, IPR:IPR004137, IPR: IPR009051, IPR: IPR011254, IPR: IPR016099 P18335 PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4, PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2, 6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 2/3, TAXON: Bacteria, TAXON: Proteobacteria,...”
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...Aminotransferase family (P36839, P94427, P53555, P71084, P30949, P33189, O34662, P38021, P22256, P50457, P12995, P23893, P48247, P18335, P77581, O30508, Q9I606, Q9I6 M4, Q9I693, Q9I700, Q9I6J2, Q9I6R7, Q9HV04, Q9HTP1, Q9HWU0, Q9HT50, P48247, Q9I168, P12677, Q82 MM1, P21267, P40732, Q8ZPV2, Q8ZLX7), Thiamine Diphosphate Decarboxylase family (P96591, P37251, P23970, Q04789,...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...5.21/27,292.02 4.52/30,891 (DIGE 4.5-6.5) ArgD P18335 5.80/43,635.78 5.75/41,241 (DIGE 4.5-6.5) 5.19/33,559 (5-6) ArgF P06960 Acetylornithine/...”
- Novel archaeal alanine:glyoxylate aminotransferase from Thermococcus litoralis
Sakuraba, Journal of bacteriology 2004 - “...P25409 NP_418478a P04694 P06986 P07172 P16246 P17736 P17731 P18335 P18544 P04181 P07991 P28269 P22256 P17649 P14010 P19938 P00510 P21549 P23721 P19689 P10658...”
1vefA / Q5SHH5 Acetylornithine aminotransferase from thermus thermophilus hb8
43% identity, 96% coverage
- Ligand: pyridoxal-5'-phosphate (1vefA)
PMT0331 Acetylornithine and succinylornithine aminotransferases from Prochlorococcus marinus str. MIT 9313
45% identity, 89% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...P9211_10217 P9211_07012 Prochlorococcus marinus MIT 9312 PMT9312_1779 PMT9312_1397, PMT9312_1565 PMT9312_0337 Prochlorococcus marinus MIT 9313 PMT2214 PMT0331, PMT1493 PMT0191 Prochlorococcus marinus MED 4 PMM1686 PMM1301, PMM1472 PMM0331 Prochlorococcus marinus NATL 2A PMN2A_1287 PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605 Syncc9605_1082, Syncc9605_2591 Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp....”
- “...P9211_10217 P9211_07012 Prochlorococcus marinus MIT 9312 n.d. PMT9312_1397, PMT9312_1565 PMT9312_0337 Prochlorococcus marinus MIT 9313 n.d. PMT0331, PMT1493 PMT0191 Prochlorococcus marinus MED 4 n.d. PMM1301, PMM1472 PMM0331 Prochlorococcus marinus NATL 2A n.d. PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605 n.d. Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC...”
lysJ / Q93R93 L-2-aminoadipate semialdehyde transaminase monomer (EC 2.6.1.118) from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
LYSJ_THET2 / Q93R93 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
Q93R93 [amino group carrier protein]-gamma-(L-lysyl)-L-glutamate aminotransferase (EC 2.6.1.118) from Thermus thermophilus (see 2 papers)
TTC1393 No description from Thermus thermophilus HB27
43% identity, 94% coverage
- function: Catalyzes the transfer of the amino group of L-glutamate to [LysW]-aminoadipate 6-semialdehyde, generating [LysW]-gamma-L-lysine (Probable). In vitro, can use N(2)-acetyl-L-ornithine and N(2)-acetyl- L-lysine (PubMed:11489859).
catalytic activity: [amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L- glutamate + 2-oxoglutarate = [amino-group carrier protein]-C- terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + L-glutamate (RHEA:41952)
cofactor: pyridoxal 5'-phosphate (Binds 1 pyridoxal phosphate per subunit.)
subunit: Homodimer.
disruption phenotype: Auxotroph for lysine. - Evolution of lysine biosynthesis in the phylum deinococcus-thermus
Nishida, International journal of evolutionary biology 2012 - “...Enzyme 6 Enzyme 7 Enzyme 8 Enzyme 9 Enzyme 10 Thermus thermophilus HB27 TTC0043 TTC1012 TTC1393 TTC1396 TTC1541* TTC1542* TTC1543* TTC1546* TTC1547* TTC1550* Thermus thermophilus HB8 TTHA0411 TTHA1378 TTHA1755 TTHA1757 TTHA1903* TTHA1904* TTHA1907* TTHA1910* TTHA1911* TTHA1914* Thermus scotoductus TSC_c05810 TSC_c20650 TSC_c03550 TSC_c3520 TSC_c01940* TSC_c01930* TSC_c01920* TSC_c01890*...”
- Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea
Pfeiffer, Genes 2021 - “...] 27566549 only for Arg, not for Lys biosynthesis 2a HVO_0043 argD no 45% TT_C1393 Q93R93 [ 130 ] 11489859 for Arg, not for Lys biosynthesis 2a HVO_0043 (cont.) yes/no 40% Saci_0755 Q4JAP8 [ 126 ] 23434852 only for Arg, not for Lys biosynthesis 2a HVO_0043...”
- GapMind: Automated Annotation of Amino Acid Biosynthesis
Price, mSystems 2020 - “...that is involved in lysine biosynthesis ([LysW]-aminoadipate semialdehyde transaminase from Thermus thermophilus ; Swiss-Prot entry Q93R93 ), which creates some uncertainty about its role. The final gap was argininosuccinate synthase (ArgH). BT3760 was identified as a moderate-confidence candidate because it is less than 40% identical to...”
YPO1962 succinylornithine aminotransferase from Yersinia pestis CO92
43% identity, 91% coverage
- Differential Gene Expression Patterns of Yersinia pestis and Yersinia pseudotuberculosis during Infection and Biofilm Formation in the Flea Digestive Tract
Chouikha, mSystems 2019 - “...response regulator 3.2 3.9 YPTB1934 YPO1936 Aminotransferase 2.0 7.8 YPTB1937 YPO1939 Glutaminase 4.8 3.3 YPTB1959 YPO1962 argD Succinylornithine/acetylornithine transaminase 2.2 2.2 YPTB3107 YPO0863 YaeF/YiiX family of permuted papain-like enzymes 2.5 2.1 YPTB3361 YPO0700 Fimbrial protein 2.1 2.4 YPTB3534 YPO3699 PliI-like periplasmic lysozyme inhibitor 2.3 9.7 YPTB3582...”
- Studying Salmonellae and Yersiniae host-pathogen interactions using integrated 'omics and modeling
Ansong, Current topics in microbiology and immunology 2013 - “...missing genes. A search for paralogs of YPO0170, the missing gene in lysine biosynthesis, uncovered YPO1962, a potential open reading frame with 59 % nucleotide identity that might have the same catalytic ability; however, there were no apparent paralogs for fabI, the missing gene in fatty...”
- An experimentally-supported genome-scale metabolic network reconstruction for Yersinia pestis CO92
Charusanti, BMC systems biology 2011 - “...step in the nine-step lysine biosynthesis pathway, but argD (YPO0170) is disrupted in YP CO92. YPO1962 has the greatest homology to YPO0170 within the Y. pestis CO92 genome, and we hypothesize here that YPO1962 can replace the function of YPO0170. Note: although each reaction in each...”
- “...genome that have homology to both YPO0170 fragments. The gene with the greatest homology was YPO1962 with 59% nucleotide identity, and we have assumed in the model that YPO1962 can replace YPO0170 in the lysine biosynthetic pathway (Figure 2B ). Phenotype simulations Once all critical gaps...”
- Characterization of Zur-dependent genes and direct Zur targets in Yersinia pestis
Li, BMC microbiology 2009 - “...to +52 YPO1963 astA -44 to -26 AAAGTTACGTCGTAACGTT 8.2 -165 to +124 -165 to +124 YPO1962 astC -478 to -460 AATATTATTACATAACCGT 4.4 -498 to -2 --- YPO2374 rovA --- --- 0 -493 to -87 --- YPO2367 gst --- --- 0 --- -571 to -217 , The...”
bglu_1g10100 Succinylornithine transaminase from Burkholderia glumae BGR1
42% identity, 93% coverage
- Identification of potential genetic components involved in the deviant quorum-sensing signaling pathways of Burkholderia glumae through a functional genomics approach
Chen, Frontiers in cellular and infection microbiology 2015 - “...production . Name of random mutants Locus of inserted genes Function of disrupted genes LSUPB186 bglu_1g10100 Succinylornithine transaminase LSUPB187 bglu_2g10840 * putative LysM domain-containing protein LSUPB182 bglu_1g02180 Diguanylate cyclase LSUPB183 bglu_1g01780 * Flagellar transcriptional activator FlhD LSUPB184 bglu_2g07160 * Catechol 1,2-dioxygenase LSUPB185 bglu_1g00380 General secretory pathway...”
- RNAseq-based Transcriptome Analysis of Burkholderia glumae Quorum Sensing
Kim, The plant pathology journal 2013 - “...crossover recombination was used for mutagenesis of BGR1. For mutagenesis, the part of the astC (bglu_1g10100) was amplified and inserted into pVIK112. To insert the part of the target gene to pVIK112, EcoR I sequence was added to forward primer and Kpn I sequence was added...”
- “...in QS-dependent pathways such as AST pathway and phosphate transport. We selected each gene, astC (bglu_1g10100) and pstA (bglu_1g11470) corresponding to a key gene in the AST pathway and in phosphate transport, respectively. We did not observe any phenotypic differences between BGR1 and pstA mutant strains....”
YPTS_2011 succinylornithine transaminase family protein from Yersinia pseudotuberculosis PB1/+
YPK_2229 succinylornithine transaminase family protein from Yersinia pseudotuberculosis YPIII
43% identity, 84% coverage
- Impact of MgtC on the Fitness of Yersinia pseudotuberculosis
Li, Pathogens (Basel, Switzerland) 2023 - “...transporter substrate-binding protein 6.125791 1.59 10 6 YPTS_3843 Malate synthase A 5.255804 1.16 10 10 YPTS_2011 Aspartate aminotransferase family protein 5.083721 1.85 10 7 YPTS_3842 Isocitrate lyase 5.081315 4.39 10 9 YPTS_2313 Acid shock protein 3.712933 8.92 10 24 YPTS_0225 Glycerol-3-phosphate dehydrogenase subunit GlpB 3.838456 7.86...”
- Genome-Scale Mapping Reveals Complex Regulatory Activities of RpoN in Yersinia pseudotuberculosis
Mahmud, mSystems 2020 - “...3.5 5.2 TTGGAATAGTTGATGCAT 9.94 54,70 YPK_2070 39 IGS14 2473234 4.3 NA 4.5 GTGGCACGAAAGCTGCTG 10 54,24,38,70 YPK_2229 E,S 12, 38, 52 IGS15 2990747 16.9 7.7 8.0 ATGGCATCTTATTTGCTC + 10.51 54,38,70 YPK_2707 6, 31 IGS16 3172389 16.6 14.6 11.7 TTGGCATGGCAATTGCGC 10.63 NA YPK_2873 NA IGS17 3205379 15.4 15.3...”
- “...with orthologs in E. coli , S . Typhimurium, and V. cholerae , for example, YPK_2229, YPK_2908, and YPK_1600, with orthologs in E. coli and S. Typhimurium ( Table3 ). There were also many novel binding sites identified in Y. pseudotuberculosis , where a majority were...”
CYA_1537 acetylornithine transaminase from Synechococcus sp. JA-3-3Ab
44% identity, 90% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...Syc1030_d Synechococcus elongatus sp. PCC 7942 n.d. Synpcc7942_0943, Synpcc7942_0031 Synpcc7942_0489 Synechococcus Yellowstone sp. JA-3-3-AB CYA_0859 CYA_1537, CYA_0689 CYA_0364 Synechococcus Yellowstone sp. JA-2-3Ba (2-13) CYB_1744 CYB_1419, CYB_2128 CYB_0516, CYB_0715, CYB_1893 Thermosynechococcus elongatus BP-1 n.d. Tlr1328, Tlr0408, Tll1935 Tlr0416, Tlr0221 Synechocystis sp. PCC 6803 Sll1077, Sll0228 Slr1022 Sll1561,...”
- “...Syc1030_d Synechococcus elongatus sp. PCC 7942 n.d. Synpcc7942_0943, Synpcc7942_0031 Synpcc7942_0489 Synechococcus Yellowstone sp. JA-3-3-AB n.d. CYA_1537, CYA_0689 CYA_0364 Synechococcus Yellowstone sp. JA-2-3Ba (2-13) CYB_0250 CYB_1419, CYB_2128 CYB_0516, CYB_0715, CYB_1893 Thermosynechococcus elongatus BP-1 Tll0507 Tlr1328, Tlr0408, Tll1935 Tlr0416, Tlr0221 Synechocystis sp. PCC 6803 Sll1336 Slr1022 Sll1561, Slr0370,...”
NGO_0646 aspartate aminotransferase family protein from Neisseria gonorrhoeae FA 1090
41% identity, 95% coverage
PP_4481 aspartate aminotransferase family protein from Pseudomonas putida KT2440
PP4481 acetylornithine aminotransferase from Pseudomonas putida KT2440
42% identity, 92% coverage
PGA1_c24230 aspartate aminotransferase family protein from Phaeobacter inhibens DSM 17395
42% identity, 96% coverage
WH5701_07406 acetylornithine aminotransferase from Synechococcus sp. WH 5701
46% identity, 87% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...sp. WH 7805 WH7805_06086, WH7805_09974 WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416 Synechococcus sp. WH 5701 WH5701_03684, WH5701_03860 WH5701_07406, WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 RS9917_06190 RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera watsonii WH 8501 n.d. CwatDRAFT_5161 CwatDRAFT_0865, CwatDRAFT_0842, CwatDRAFT_0969 Trichodesmium erythraeum IMS 101 TeryDRAFT_4567 TeryDRAFT_3251 TeryDRAFT_2672 TeryDRAFT_3296, TeryDRAFT_3923 Freshwater species...”
- “...SYNW1956 Synechococcus sp. WH 7805 n.d. WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416 Synechococcus sp. WH 5701 n.d. WH5701_07406, WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 n.d. RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera watsonii WH 8501 CwatDRAFT_0830 CwatDRAFT_5161 CwatDRAFT_0865, CwatDRAFT_0842, CwatDRAFT_0969 Trichodesmium erythraeum IMS 101 TeryDRAFT_2282 TeryDRAFT_3251 TeryDRAFT_2672 TeryDRAFT_3296, TeryDRAFT_3923 Freshwater species...”
DET1258 acetylornithine aminotransferase from Dehalococcoides ethenogenes 195
43% identity, 95% coverage
VC2618 acetylornithine aminotransferase from Vibrio cholerae O1 biovar eltor str. N16961
42% identity, 93% coverage
- cAMP Receptor Protein Controls Vibrio cholerae Gene Expression in Response to Host Colonization
Manneh-Roussel, mBio 2018 - “...1.5E03 GGTGATGGTCGCCACT pyrB No 2743349 2743361.5 8.1E04 ATCGCGTCACATCACA VC2561<> cpdB No 2787939 2787903.5 3.0E04 TGAGATAAACCCCACA VC2618 Yes 2845246 2845280.5 5.7E07 TGTGATTTTCATCACG VC2677 No 2864757 2864763.5 9.2E04 ATAGATAAAACTCTCA VC2698<> aspA Yes 2933468 2933432.5 7.5E04 TTTGATTATCATCAAC 16sg ND 2936869 2936904.5 3.1E04 TTCGATACCAAGCACA 23Sh ND ChromosomeII 12067 12085.5 5.4E08...”
- Conservation of the binding site for the arginine repressor in all bacterial lineages
Makarova, Genome biology 2001 - “...VC2316 -15 3.82 AaaGAATAAAAAgTCATT VC2390-89 -52 3.57 ttTGcAaAAtAATTtATT VC2508 -72 3.72 ttaaAATAtttATTCAcT -51 3.35 AtaGcATtttcATgCtTT VC2618 -120 3.83 AaTaAATgtAAATaCAaT VC2644-42 -76 3.00 AacacATAttAAaTCAcT -55 4.00 AGTGAATAAAAAaaCAaT VC2645 -79 3.50 AtTGttTttttATTCAcT -58 3.27 AGTGAtTtAAtATgtgTT VCA0757-60 -74 3.00 ttTtggTttttATaCATT -53 3.85 AtTGcATAAAAATaCgTT B. subtilis argCJBD -64 5.22 ATtAATTTtTATTCAT...”
TM1785 acetylornithine aminotransferase from Thermotoga maritima MSB8
43% identity, 97% coverage
- Acetylornithine aminotransferase TM1785 performs multiple functions in the hyperthermophile Thermotoga maritima
Miyamoto, FEBS letters 2021 (PubMed)- “...of d-amino acid metabolism remains limited. Herein, we identified and characterized T. maritima acetylornithine aminotransferase TM1785. The enzyme was most active towards acetyl-l-ornithine, but also utilized l-glutamate, l-ornithine and acetyl-l-lysine as amino donors, and 2-oxoglutarate was the preferred amino acceptor. TM1785 also displayed racemase activity towards...”
- “...Catalytic efficiency (kcat /Km ) was highest for aminotransferase activity and lowest for racemase activity. TM1785 is a novel acetylornithine aminotransferase associated with l-arginine biosynthesis that possesses two additional distinct activities....”
2ordA / Q9X2A5 Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
43% identity, 95% coverage
- Ligand: pyridoxal-5'-phosphate (2ordA)
PMI_RS13935 aspartate aminotransferase family protein from Proteus mirabilis HI4320
PMI2821 acetylornithine/succinyldiaminopimelate aminotransferase from Proteus mirabilis HI4320
43% identity, 93% coverage
SAR11_0502 Acetylornithine aminotransferase from Candidatus Pelagibacter ubique HTCC1062
43% identity, 97% coverage
CYB_1419 acetylornithine transaminase from Synechococcus sp. JA-2-3B'a(2-13)
44% identity, 86% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...Synpcc7942_0489 Synechococcus Yellowstone sp. JA-3-3-AB CYA_0859 CYA_1537, CYA_0689 CYA_0364 Synechococcus Yellowstone sp. JA-2-3Ba (2-13) CYB_1744 CYB_1419, CYB_2128 CYB_0516, CYB_0715, CYB_1893 Thermosynechococcus elongatus BP-1 n.d. Tlr1328, Tlr0408, Tll1935 Tlr0416, Tlr0221 Synechocystis sp. PCC 6803 Sll1077, Sll0228 Slr1022 Sll1561, Slr0370, Slr0091 Gloeobacter violaceus PCC 7421 n.d. Glr0547, Glr3849,...”
- “...Synpcc7942_0489 Synechococcus Yellowstone sp. JA-3-3-AB n.d. CYA_1537, CYA_0689 CYA_0364 Synechococcus Yellowstone sp. JA-2-3Ba (2-13) CYB_0250 CYB_1419, CYB_2128 CYB_0516, CYB_0715, CYB_1893 Thermosynechococcus elongatus BP-1 Tll0507 Tlr1328, Tlr0408, Tll1935 Tlr0416, Tlr0221 Synechocystis sp. PCC 6803 Sll1336 Slr1022 Sll1561, Slr0370, Slr0091 Gloeobacter violaceus PCC 7421 Glr1758 Glr0547, Glr3849, Gll2223...”
WH7805_05656 acetylornithine aminotransferase from Synechococcus sp. WH 7805
44% identity, 93% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...Synechococcus sp. WH 8102 SYNW1412, SYNW2422 SYNW1634, SYNW0629 SYNW1956 Synechococcus sp. WH 7805 WH7805_06086, WH7805_09974 WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416 Synechococcus sp. WH 5701 WH5701_03684, WH5701_03860 WH5701_07406, WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 RS9917_06190 RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera watsonii WH 8501 n.d. CwatDRAFT_5161 CwatDRAFT_0865, CwatDRAFT_0842, CwatDRAFT_0969 Trichodesmium...”
- “...Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH 8102 n.d. SYNW1634, SYNW0629 SYNW1956 Synechococcus sp. WH 7805 n.d. WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416 Synechococcus sp. WH 5701 n.d. WH5701_07406, WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 n.d. RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera watsonii WH 8501 CwatDRAFT_0830 CwatDRAFT_5161 CwatDRAFT_0865, CwatDRAFT_0842, CwatDRAFT_0969 Trichodesmium erythraeum...”
YE2469 succinylornithine aminotransferase from Yersinia enterocolitica subsp. enterocolitica 8081
42% identity, 92% coverage
- Comparison of Yersinia enterocolitica DNA Methylation at Ambient and Host Temperatures
Van, Epigenomes 2023 - “...from TSS R putative cyclic-di-GMP phosphodiesterase. 2435785 55 from TSS 2435803 73 from TSS 2656973 YE2469 369 from TSS MT ArgM, bifunctional succinylornithine transaminase/acetylornithine transaminase 2857854 YE2639 318 from TSS M putative transporter protein 3351041 YE3081 ORF MT WbcD, putative 6-deoxy-D-Gul transferase YE3080 191 from TSS...”
TSC_c03550 [LysW]-aminoadipate semialdehyde transaminase LysJ from Thermus scotoductus SA-01
42% identity, 93% coverage
- Evolution of lysine biosynthesis in the phylum deinococcus-thermus
Nishida, International journal of evolutionary biology 2012 - “...HB8 TTHA0411 TTHA1378 TTHA1755 TTHA1757 TTHA1903* TTHA1904* TTHA1907* TTHA1910* TTHA1911* TTHA1914* Thermus scotoductus TSC_c05810 TSC_c20650 TSC_c03550 TSC_c3520 TSC_c01940* TSC_c01930* TSC_c01920* TSC_c01890* TSC_c01880* TSC_c01850* Meiothermus ruber Mrub_0871 Mrub_2738 Mrub_0027 Mrub_2721* Mrub_2723* Mrub_2724* Mrub_2727* Mrub_2728* Meiothermus silvanus Mesil_2567 Mesil_1337 Mesil_0348 Mesil_0347 Mesil_0435* Mesil_0436* Mesil_0438* Mesil_0441* Mesil_0442* Oceanithermus profundus...”
ACIAD1284 succinylornithine transaminase (also has acetylornitine transaminase activity, PLP-dependent) (carbon starvation protein C) from Acinetobacter sp. ADP1
43% identity, 94% coverage
- Characterization of a novel β-alanine biosynthetic pathway consisting of promiscuous metabolic enzymes
Perchat, The Journal of biological chemistry 2022 - “...l -glutamate formation with l -glutamate dehydrogenase. We detected activity for three -transaminases: ACIAD3446 (GabT), ACIAD1284 (AstC), and ACIAD1210 (Dat). Specific activities were 94, 3.4, and 5.9nmolmin 1 mg 1 , respectively (see the Experimental procedures section). GabT, AstC, and Dat were purified for further characterization,...”
- iTRAQ-Based Comparative Proteomic Analysis of Acinetobacter baylyi ADP1 Under DNA Damage in Relation to Different Carbon Sources
Jiang, Frontiers in microbiology 2019 - “...0.8 0.74 Gamma-glutamyltranspeptidase precursor ggt ACIAD0929 0.34 0.77 0.5 0.41 Bifunctional succinylornithine transaminase/acetylornithine transaminase argD ACIAD1284 0.4 0.32 0.15 0.26 Arginine N -succinyltransferase astA ACIAD1286 0.48 0.63 0.76 0.63 Succinylglutamate desuccinylase astE ACIAD1289 0.17 0.82 0.69 0.66 Amidase amdA ACIAD1618 0.68 0.06 0.58 0.27 Acetyl-CoA acetyltransferase...”
B8CGH5 acetylornithine transaminase from Thalassiosira pseudonana
THAPSDRAFT_270136 n-acetylornithine aminotransferase from Thalassiosira pseudonana CCMP1335
42% identity, 78% coverage
SPISAL_06590 acetylornithine transaminase from Spiribacter salinus M19-40
44% identity, 94% coverage
- Compatible Solute Synthesis and Import by the Moderate Halophile Spiribacter salinus: Physiology and Genomics
León, Frontiers in microbiology 2018 - “...of the S. salinus M19-40 genome. In this way, we found three EctB-related proteins: SPISAL_02400, SPISAL_06590, and SPISAL_07805 ( Len et al., 2013 ; Lopez-Perez et al., 2013 ). To distinguish the potential EctB enzyme from the two other transaminases, we used the orthology IDs from...”
- “...SPISAL_07805 protein belongs to the orthology group K01845-hemL that comprises enzymes involved in heme biosynthesis. SPISAL_06590 belongs the orthology group K00821-argD comprises enzymes involved in arginine biosynthesis, and indeed the gene encoding SPISAL_06590 is flanked by a gene that is annotated in the S. salinus M19-40...”
ARGD_ARATH / Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
NP_178175 HOPW1-1-interacting 1 from Arabidopsis thaliana
AT1G80600 WIN1 (HOPW1-1-INTERACTING 1); N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase/ catalytic/ pyridoxal phosphate binding / transaminase from Arabidopsis thaliana
43% identity, 82% coverage
- function: Involved in the biosynthesis of citrulline (By similarity). Essential gene that modulates defense response to pathogenic bacteria, conferring susceptibility and repressing salicylic acid (SA) accumulation.
catalytic activity: N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L- glutamate 5-semialdehyde + L-glutamate (RHEA:18049)
cofactor: pyridoxal 5'-phosphate
subunit: Interacts with the P.syringae pv. maculicola effector HopW1-1 (via C-terminus). - The Arabidopsis TUMOR PRONE5 gene encodes an acetylornithine aminotransferase required for arginine biosynthesis and root meristem maintenance in blue light.
Frémont, Plant physiology 2013 - GeneRIF: TUMOR PRONE5 (TUP5) gene of Arabidopsis codes for acetylornithine aminotransferase that catalyzes the fourth step of arginine biosynthesis and is required for root meristem maintenance in blue light. [TUP5]
- Overexpression of AtSHN1/WIN1 provokes unique defense responses.
Sela, PloS one 2013 - GeneRIF: Overexpression of AtSHN1/WIN1 provokes unique defense responses.
- Identification of SNPs Associated with Drought Resistance in Hybrid Populations of Picea abies (L.) H. Karst.-P. obovata (Ledeb.).
Vasileva, Genes 2024 - “...ornithine aminotransferase (GenBank accession XM_058003929). This protein is involved in amino acid metabolism (UniProt accession Q9M8M7), which explains its high conservatism. Functionally, ornithine aminotransferase links the stress response and nitrogen metabolism and is potentially an important element in maintaining homeostasis and regulating amino acid interconversions in...”
- Proteome-wide analysis of hydrogen peroxide-induced protein carbonylation in Arabidopsis thaliana
Fangue-Yapseu, Frontiers in plant science 2022 - “...2425.17 2 2 2 2 2 2 2 3 3 8 AT1G80600 Acetylornithine aminotransferase (WIN1) Q9M8M7 48.83 Plastid/ Mitochondrion (R)VFFCNSGTEANEAAIK(F) (K)IAAVFVEPIQGEGGIYSATK(E) (R)SACDAAGSLLVFDEVQCGLGR(T) (R)DSGLLILTAGK(G) (R)IVPPLVISEEEIER(A) (K)EYLDCASGIAVNALGHGDPDWLR(A) (K)VIVGTYAR(A) 1756.81 2049.08 2224.03 1086.63 1621.90 2528.18 877.50 2 2 2 2 2 3 2 18 AT2G37220 RNA-binding protein CP29B Q9ZUU4...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...small subunit 1 58 Q9FFF4, At5g16290 Acetylglutamate kinase 92 Q9SCL7, At3g57560 Acetylornithine aminotransferase 62, 63 Q9M8M7, At1g80600 Argininosuccinate synthase 51, 52 Q9SZX3, At4g24830 ATP phosphoribosyltransferase 1 62 Q9S762, At1g58080 Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase 30 Q9SQT8, At3g06350 Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase 64, 66 Q9SIE1, At2g22250...”
- Changes in annual transcriptome dynamics of a clone of Japanese cedar (Cryptomeria japonica D. Don) planted under different climate conditions
Nose, PloS one 2023 - “...CUST_5816_PI429951308 reCj20419:MSWR:isotig20271 AT5G52920 0.0 PKP-BETA1 plastidic pyruvate kinase beta subunit 1 temp-0h -0.90 CUST_6317_PI429951308 reCj20922:-SWR:isotig20774 AT1G80600 0.0 WIN1 HOPW1-1-interacting 1 temp-min -0.89 CUST_5166_PI429951308 reCj19763:MSWR:isotig19615 AT3G10050 0.0 OMR1 L-O-methylthreonine resistant 1 temp-7d -0.87 CUST_7163_PI429951308 reCj21779:MSWR:isotig21631 AT4G13430 0.0 IIL1 isopropyl malate isomerase large subunit 1 temp-7d -0.86 CUST_11543_PI429951308...”
- Proteome-wide analysis of hydrogen peroxide-induced protein carbonylation in Arabidopsis thaliana
Fangue-Yapseu, Frontiers in plant science 2022 - “...895.520 1699.92 1079.57 2548.24 2425.17 2 2 2 2 2 2 2 3 3 8 AT1G80600 Acetylornithine aminotransferase (WIN1) Q9M8M7 48.83 Plastid/ Mitochondrion (R)VFFCNSGTEANEAAIK(F) (K)IAAVFVEPIQGEGGIYSATK(E) (R)SACDAAGSLLVFDEVQCGLGR(T) (R)DSGLLILTAGK(G) (R)IVPPLVISEEEIER(A) (K)EYLDCASGIAVNALGHGDPDWLR(A) (K)VIVGTYAR(A) 1756.81 2049.08 2224.03 1086.63 1621.90 2528.18 877.50 2 2 2 2 2 3 2 18 AT2G37220...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...subunit 1 58 Q9FFF4, At5g16290 Acetylglutamate kinase 92 Q9SCL7, At3g57560 Acetylornithine aminotransferase 62, 63 Q9M8M7, At1g80600 Argininosuccinate synthase 51, 52 Q9SZX3, At4g24830 ATP phosphoribosyltransferase 1 62 Q9S762, At1g58080 Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase 30 Q9SQT8, At3g06350 Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase 64, 66 Q9SIE1, At2g22250 Branched-chain-amino-acid...”
- “...N-acetylglutamyl-phosphate reductase (AGPR, At2g19940, spots 70 and 71). In the next step, acetylornithine aminotransferase (WIN1, At1g80600), detected in spots 62 and 63, transfers an amino group from a second glutamate to form N 2 -acetylornithine. Subsequently, the produced ornithine is further used by ornithine transcarbamomylase (OTC,...”
- Quantitative Proteomic Analysis of the Response to Zinc, Magnesium, and Calcium Deficiency in Specific Cell Types of Arabidopsis Roots
Fukao, Proteomes 2016 - “...9 0.41 0.58 N.D. N.D. AT1G78800 UDP-Glycosyltransferase superfamily protein 0.82 0.74 0.34 0.58 1.00 0.89 AT1G80600 HOPW1-1-interacting 1 0.36 0.62 0.83 0.72 0.69 0.64 AT2G04305 Magnesium transporter CorA-like family protein N.D. 0.32 0.46 N.D. AT2G17130 isocitrate dehydrogenase subunit 2 0.47 0.41 0.44 0.63 0.63 0.89 AT2G28190...”
- Physiological implications of arginine metabolism in plants
Winter, Frontiers in plant science 2015 - “...demonstrated by complementation studies as well as immunoblot analysis. The TUMOR PRONE5 ( TUP5 , At1g80600) gene of Arabidopsis was demonstrated to encode a NAOAT ( Frmont et al., 2013 ). Characterization of the gene and its mutant lines showed a strongly reduced free arginine content...”
- DYn-2 Based Identification of Arabidopsis Sulfenomes
Akter, Molecular & cellular proteomics : MCP 2015 - “...AT1G22410 AT5G16290 AT3G53580 AT2G29690 AT2G22250 AT1G80600 AT1G58080 AT1G48850 AT2G31810 AT2G45300 SERINE HYDROXYMETHYLTRANSFERASE 3 THREONINE SYNTHASE...”
- Jacks of metal/metalloid chelation trade in plants-an overview
Anjum, Frontiers in plant science 2015 - “...(2 mM) At5g19530 Induction of Spm/Spd synthase family protein Cs tolerance Sharma and Dietz, 2006 At1g80600 Induction of acetylornithine aminotransferase, mitochondrial, putative/acetylornithine transaminase, putative/AOTA At5g46180 Induction of Orn aminotransferase, putative/Orn-oxo-acid aminotransferase Arabidopsis thaliana Pb(NO 3 ) 2 (25, 50 ppm) At5g19530 Induced Spm/Spd synthase family protein...”
lpg2968 N-acetylornithine aminotransferase ArgD from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
42% identity, 97% coverage
PD0654 succinylornithine aminotransferase from Xylella fastidiosa Temecula1
44% identity, 91% coverage
Syncc9605_0858 acetylornithine and succinylornithine aminotransferases from Synechococcus sp. CC9605
43% identity, 88% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...PMM0331 Prochlorococcus marinus NATL 2A PMN2A_1287 PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605 Syncc9605_1082, Syncc9605_2591 Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC 9902 Syncc9902_2230 Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH 8102 SYNW1412, SYNW2422 SYNW1634, SYNW0629 SYNW1956 Synechococcus sp. WH 7805 WH7805_06086, WH7805_09974 WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416...”
- “...PMM1472 PMM0331 Prochlorococcus marinus NATL 2A n.d. PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605 n.d. Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC 9902 n.d. Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH 8102 n.d. SYNW1634, SYNW0629 SYNW1956 Synechococcus sp. WH 7805 n.d. WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416 Synechococcus sp....”
D4I307 Acetylornithine/succinyldiaminopimelate aminotransferase from Erwinia amylovora (strain CFBP1430)
EAMY_3415 bifunctional acetylornithine delta-aminotransferase / N-succinyldiaminopimelate aminotransferase from Erwinia amylovora CFBP1430
41% identity, 93% coverage
- Novel Virulence Factors Deciphering Klebsiella pneumoniae KpC4 Infect Maize as a Crossing-Kingdom Pathogen: An Emerging Environmental Threat
Huang, International journal of molecular sciences 2022 - “...pump E. amylovora Q7WTQ9 Fire blight Apple KpC4_4696 argD 78.71 N-acetylornithine aminotransferase enzyme E. amylovora D4I307 Fire blight Pear/apple KpC4_0520 rsmAXoo 82.14 RNA-binding protein Xanthomonas oryzae pv. Oryzae E2J5T5 Bacterial leaf blight Rice KpC4_2459 iutA 75.93 Siderophore-mediated iron acquisition Pantoea stewartii H3RJF2 Stewart wilt of sweet...”
- Evaluating Eucalyptus leaf colonization by Brasilonema octagenarum (Cyanobacteria, Scytonemataceae) using in planta experiments and genomics
Alvarenga, PeerJ 2020 - “...and starvation response + + D4HXR8 AcrD resistance-nodulation-cell division transport + + + + + D4I307 ArgD N-acetylornithine aminotransferase + + + F5HCK8 IPMDH 3-isopropylmalate dehydrogenase + + + + + G4ML75 MET6 methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase + + + G4MTK2 MoARG1 arginine biosynthesis + + + +...”
- “...translated protein sequences from the Brasilonema genomes and proteins deposited in PHI-base ( C5A9K4 , D4I307 , F5HCK8 , G4ML75 , G4MTK2 , Q4UTV7 , Q4UUL4 , Q8XSV8 , Q8Y0J2 and Q9C1T0 ) are likely to indicate enzymes with roles in primary metabolism ( Cooley et...”
- The stringent response regulator (p) ppGpp mediates virulence gene expression and survival in Erwinia amylovora
Yang, BMC genomics 2020 - “...8.35 EAMY_0146 argH , argininosuccinate lyase 1.88 10.84 EAMY_0145 argG , argininosuccinate synthase 1.83 11.69 EAMY_3415 argD , 4-aminobutyrate aminotransferase 1.83 10.43 EAMY_2082 argS , arginyl-tRNA synthetase 1.37 8.58 EAMY_1631 astB , succinylarginine dihydrolase 3.05 6.66 EAMY_1630 astD , NAD-dependent aldehyde dehydrogenase 1.98 6.45 EAMY_1628 astC...”
A7ME32 Acetylornithine/succinyldiaminopimelate aminotransferase from Cronobacter sakazakii (strain ATCC BAA-894)
42% identity, 90% coverage
aruC / O30508 succinylornithine transaminase subunit (EC 2.6.1.13; EC 2.6.1.11; EC 2.6.1.81) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
ARUC_PSEAE / O30508 Succinylornithine transaminase/acetylornithine aminotransferase; ACOAT; SOAT; Succinylornithine aminotransferase; EC 2.6.1.11; EC 2.6.1.81 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
O30508 succinylornithine transaminase (EC 2.6.1.81) from Pseudomonas aeruginosa (see paper)
PA0895 N-succinylglutamate 5-semialdehyde dehydrogenase from Pseudomonas aeruginosa PAO1
41% identity, 92% coverage
- function: Transaminates both N(2)-acetylornithine and N(2)- succinylornithine
catalytic activity: N(2)-succinyl-L-ornithine + 2-oxoglutarate = N-succinyl-L- glutamate 5-semialdehyde + L-glutamate (RHEA:16953)
catalytic activity: N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L- glutamate 5-semialdehyde + L-glutamate (RHEA:18049)
cofactor: pyridoxal 5'-phosphate (Binds 1 pyridoxal phosphate per subunit.)
subunit: Homodimer. - Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...(P36839, P94427, P53555, P71084, P30949, P33189, O34662, P38021, P22256, P50457, P12995, P23893, P48247, P18335, P77581, O30508, Q9I606, Q9I6 M4, Q9I693, Q9I700, Q9I6J2, Q9I6R7, Q9HV04, Q9HTP1, Q9HWU0, Q9HT50, P48247, Q9I168, P12677, Q82 MM1, P21267, P40732, Q8ZPV2, Q8ZLX7), Thiamine Diphosphate Decarboxylase family (P96591, P37251, P23970, Q04789, P42415, P07003,...”
- Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa
Kawalek, Microbiology spectrum 2023 - “...CoIP by BACTH, confirmed several enzymes as ParB partners ( Fig.3B ). These included AruC (PA0895), a part of the catabolic arginine succinyltransferase pathway ( 48 ), as well as Pae CTP-synthase PA3637 (PyrG), and others ( Table1 , Fig.3B ). While it is tempting to...”
- “...uncovered numerous novel proteins interacting with ParB ( Table1 ). Those encompass metabolic proteins AruC (PA0895), an N2-succinylornithine 5-aminotransferase ( 48 ), GlyA3 (PA4602), NadE (PA4920), and ArcB (PA5172) ( Fig.3B ). Moreover, interactions were observed with PyrG (PA3637) encoding a CTP synthetase ( 75 )....”
- The Breadth and Molecular Basis of Hcp-Driven Type VI Secretion System Effector Delivery
Howard, mBio 2021 - “...Represses H1-T6SS 25.9 PA5220 5.3 1.32 Hypothetical 30.5 PA4748 TpiA 5.3 2.46 Triosephosphate isomerase 25.6 PA0895 AruC 5 1.73 Succinylornithine transaminase/acetylornithine aminotransferase 43.7 PA3471 MaeA 4.8 2.41 NAD-dependent malic enzyme 62.4 PA1588 SucC 4.8 2.73 Succinate-CoA ligase (ADP-forming) subunit beta 41.5 PA0317 4.8 2.02 d -2-Hydroxyglutarate...”
- Traditional Chinese Medicine Tanreqing Inhibits Quorum Sensing Systems in Pseudomonas aeruginosa
Yang, Frontiers in microbiology 2020 - “...Threonine dehydratase, biosynthetic PA0390 metX 1.7 Homoserine O -acetyltransferase PA0782 putA 1.8 Proline dehydrogenase PutA PA0895 aruC 1.4 N2-succinylornithine 5-aminotransferase (SOAT) PA0896 aruF 1.2 Subunit I of arginine N2-succinyltransferase PA0897 aruG 1.2 Subunit II of arginine N2-succinyltransferase PA1818 cadA 1.2 Lysine decarboxylase PA3082 gbt 1.2 Glycine...”
- Parallel evolutionary paths to produce more than one Pseudomonas aeruginosa biofilm phenotype
Thöming, NPJ biofilms and microbiomes 2020 - “...PA14_47400 PA1300 2.8 PA14_52690 PA0897 aruG 3.0 PA14_33260 PA2426 pvdS 3.0 PA14_49690 PA1137 3.8 PA14_52720 PA0895 argD 2.4 PA14_33270 PA2425 pvdG 4.0 PA14_50050 PA1108 2.6 PA14_56370 PA4336 2.9 PA14_33280 PA2424 pvdL 3.8 PA14_51460 PA0993 cupC2 3.2 PA14_56780 PA4366 sodB 2.6 PA14_33500 PA2413 pvdH 3.0 PA14_52960 PA0874...”
- Pseudomonas aeruginosa mutants defective in glucose uptake have pleiotropic phenotype and altered virulence in non-mammal infection models
Raneri, Scientific reports 2018 - “...rpoD 1.1 0.4 0.5 sigma factor D PA0893 argR 0.6 0.6 0.4 transcriptional regulator ArgR PA0895 aruC 0.5 0.3 0.2 acetylornithine aminotransferase PA2259 ptxS 2.9 0.5 1.4 transcriptional regulator PtxS PA2264 PA2264 3.4 0.5 1.5 conserved hypothetical protein c PA2320 gntR 3.7 0.3 1.1 GntR transcriptional...”
- Inhibition of Pseudomonas aeruginosa swarming motility by 1-naphthol and other bicyclic compounds bearing hydroxyl groups
Oura, Applied and environmental microbiology 2015 - “...Chemotaxis transducer Chemotaxis transducer Chemotaxis transducer Metabolism PA0895 PA1562 PA1984 PA2007 PA2008 PA2009 PA2014 PA2015 PA2016 PA2247 PA2248 PA2249...”
- Pseudomonas aeruginosa ceftolozane-tazobactam resistance development requires multiple mutations leading to overexpression and structural modification of AmpC
Cabot, Antimicrobial agents and chemotherapy 2014 - “...Intergenic PA0747 PA0811 yicE mgtA chpA PA0877 PA0887 PA0895 PA0902 PA3179 PA3187 PA3206 PA3346 PA3516 PA3614 PA3624 7, P1.2, TOL-TAZr 7, PmS.1, TOL-TAZr 7,...”
- Bis-(3'-5')-cyclic dimeric GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa
Chua, Antimicrobial agents and chemotherapy 2013 - “...PA3117 PA0764 PA3313 PA3190 PA4624 PA5046 PA5312 PA3330 PA0895 PA4450 PA3327 PA0482 PA3769 PA3977 PA2119 PA0552 PA4687 PA2413 PA4448 PA5172 PA3686 PA3922 PA2385...”
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BWI76_RS11670 Succinylornithine transaminase (EC 2.6.1.81) from Klebsiella michiganensis M5al
41% identity, 91% coverage
- mutant phenotype: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (SUCCORNTRANSAM-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
PA14_52720 N-succinylglutamate 5-semialdehyde dehydrogenase from Pseudomonas aeruginosa UCBPP-PA14
41% identity, 92% coverage
Tter_0321 acetylornithine and succinylornithine aminotransferase from Thermobaculum terrenum ATCC BAA-798
39% identity, 96% coverage
- Modular architecture of metabolic pathways revealed by conserved sequences of reactions
Muto, Journal of chemical information and modeling 2013 - “...RM002 2-aminoadipate lysine M00028 (bsu) BSU11200 BSU11210+BSU11190 BSU11220 glutamate ornithine M00031 (ttr) Tter_0315+Tter_0316 Tter_0320 Tter_0319 Tter_0321 Tter_0317 a KEGG organism codes are shown in parentheses: pfu (T00075), Pyrococcus furiosus DSM 3638; bth (T00122), Bacteroides thetaiotaomicrometer VPI-5482; bsu (T00010), Bacillus subtilis 168; ttr (T01134), Thermobaculum terrenum ATCC...”
A8J933 acetylornithine transaminase (EC 2.6.1.11) from Chlamydomonas reinhardtii (see paper)
XP_001698091 uncharacterized protein from Chlamydomonas reinhardtii
40% identity, 83% coverage
PS417_21710 Succinylornithine transaminase (EC 2.6.1.81) from Pseudomonas simiae WCS417
42% identity, 92% coverage
- mutant phenotype: Specifically important for: L-Arginine. succinylornithine transaminase is involved in L-arginine degradation via N-succinylarginine. Also this gene is cofit with other putative steps in that pathway (N-succinylarginine dihydrolase, succinylglutamate-semialdehyde dehydrogenase, succinylglutamate desuccinylase). In contrast, neither of the functions proposed by KEGG have an obvious relationship to arginine catabolism.
KP1_2498 putative succinylornithine transaminase from Klebsiella pneumoniae NTUH-K2044
41% identity, 94% coverage
Pro1375 Ornithine/acetylornithine aminotransferase from Prochlorococcus marinus str. SS120
42% identity, 94% coverage
FGSG_01573 acetylornithine aminotransferase from Fusarium graminearum PH-1
42% identity, 83% coverage
Pf1N1B4_3440 Succinylornithine transaminase (EC 2.6.1.81) from Pseudomonas fluorescens FW300-N1B4
43% identity, 92% coverage
- mutant phenotype: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (SUCCORNTRANSAM-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
B7GEJ6 acetylornithine transaminase from Phaeodactylum tricornutum (strain CCAP 1055/1)
40% identity, 77% coverage
XF1427 succinylornithine aminotransferase from Xylella fastidiosa 9a5c
Q9PDF2 Acetylornithine aminotransferase from Xylella fastidiosa (strain 9a5c)
XF_RS06015 acetylornithine transaminase from Xylella fastidiosa 9a5c
43% identity, 91% coverage
- In vitro Determination of Extracellular Proteins from Xylella fastidiosa
Mendes, Frontiers in microbiology 2016 - “...SecP 4 60kDa chaperonin 57.8 XF0615 XF0494 Q9PFP2 No No 0.077 Acetylornithine aminotransferase (ACOAT) 43.7 XF1427 XF1180 Q9PDF2 No No 0.097 Adenylosuccinate lyase (ASL) 50.6 XF1553 XF1289 P44797 No No 0.076 Amidohydrolase family protein 47.3 XF2472 XF2144 O66851 No No 0.069 Aminopeptidase (peptidase M28 family protein)...”
- Analysis of the biofilm proteome of Xylella fastidiosa
Silva, Proteome science 2011 - “...5.0 211 I.C.8 XF0389 two-component system, regulatory protein [Xylella fastidiosa 9a5c] 25364 5.27 404 I.D XF1427 bifunctional N-succinyldiaminopimelate-aminotransferase/acetylornithine transaminase protein [Xylella fastidiosa 9a5c] 44116 5.47 104 II.A.1 XF0114 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase [Xylella fastidiosa 9a5c] 32566 6.0 136 II.A.2 XF1822 ketol-acid reductoisomerase [Xylella fastidiosa 9a5c] 40369 6.17 127...”
- Xylella fastidiosa gene expression analysis by DNA microarrays
Travensolo, Genetics and molecular biology 2009 - “...11.04 Gluconolactonase precursor -0.60 Biosynthesis of amino acids and proteins XF2465 metA Homoserine O-acetyltransferase 0.71 XF1427 argM Succinylornithine aminotransferase 0.69 XF1944 dcp Peptidyl-dipeptidase 0.64 XF1189 lon ATP-dependent serine proteinase La 0.62 XF1116 lysA Bifunctional diaminopimelate -2.17 XF0267 pspB Serine protease -1.02 XF2219 hisD Histidinol dehydrogenase -0.81...”
- In vitro Determination of Extracellular Proteins from Xylella fastidiosa
Mendes, Frontiers in microbiology 2016 - “...60kDa chaperonin 57.8 XF0615 XF0494 Q9PFP2 No No 0.077 Acetylornithine aminotransferase (ACOAT) 43.7 XF1427 XF1180 Q9PDF2 No No 0.097 Adenylosuccinate lyase (ASL) 50.6 XF1553 XF1289 P44797 No No 0.076 Amidohydrolase family protein 47.3 XF2472 XF2144 O66851 No No 0.069 Aminopeptidase (peptidase M28 family protein) 57.7 XF0820...”
- Overexpression of mqsR in Xylella fastidiosa Leads to a Priming Effect of Cells to Copper Stress Tolerance
Carvalho, Frontiers in microbiology 2021 - “...three ribosomal subunits (XF_RS00715, XF_RS00285, XF_RS09575), the msrB (XF_RS03590) and yuxK (XF_RS04035), the aminotransferase astC (XF_RS06015, also known as argM or cstC ), and two hypothetical proteins (XF_RS03860, XF_RS05645). Interestingly though, the ribosomal protein (XF_RS12125) was the only one showing an opposite behavior, being induced in...”
NCU05410 acetylornithine aminotransferase from Neurospora crassa OR74A
41% identity, 83% coverage
- RNAseq and targeted metabolomics implicate RIC8 in regulation of energy homeostasis, amino acid compartmentation, and asexual development in <i>Neurospora crassa</i>
Quinn, mBio 2024 - “...(File S2), suggesting possible post-transcriptional regulation. Although the gna-3 mutant had reduced expression of arg-5 (NCU05410; File S2), arginine levels were normal, and glutamate (124%) and N-acetylglutamate (172%) amounts were actually elevated ( Fig. 3 ; File S3). The relative amounts of several metabolites involved in...”
- “...wild type. arg-14 = NCU07682, acetylglutamate synthase; arg-4 = NCU10468, N-acetylornithine-glutamate acetyl transferase; arg-5 = NCU05410, acetylornithine aminotransferase; arg-6 = NCU00567, acetylglutamate kinase; arg-2 = NCU07732, carbamoyl-phosphate synthase small subunit; arg-3 = NCU02677, carbamoyl-phosphate synthase large subunit; arg-12 = NCU01667, ornithine transcarbamylase; arg-1 = NCU02639, argininosuccinate...”
- Permissiveness and competition within and between Neurospora crassa syncytia
Mela, Genetics 2023 - “...numbers for the loci in this study are as follows: his-3 NCU04393; csr-1 NCU00726; arg-5 NCU05410; arg-12 NCU01667; ro-3 NCU03483; ro-10 NCU10696; tol NCU03378; cwr-1 NCU03180; cwr-2 NCU03182; rcd-1 NCU05712; so NCU02794; and fl NCU08726. Culturing and crossing, using Vogel's media ( Vogel 1964 ) and...”
- Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa
Watters, G3 (Bethesda, Md.) 2018 - “...or Biogenisis/ Cellular Process/Localization/Metabolic Process NCU07617 11254 dense aconidiate-3 acon-3 Morph/Hyph Biological Regulation/Developmental Process/ Reproduction NCU05410 16183 dense arginine-5 arg-5 E. coli CS Orth Binding Cellular Process/Metabolic Process NCU02114 11571 dense G1/S-specific cyclin Cln1 Morph/Hyph Binding Cellular Process NCU00144 11340 dense hypothetical protein Transc Factors NCU03120...”
- Use of 1H nuclear magnetic resonance to measure intracellular metabolite levels during growth and asexual sporulation in Neurospora crassa
Kim, Eukaryotic cell 2011 - “...pathways NCU01295/NCU10468 NCU00567 2.6.1.11 6.3.5.5 EC no. NCU05410 NCU07732 2.1.3.3 3.5.3.1 2.3.3.14 Broad gene no.a NCU01667 NCU02333 NCU05526 4.2.1.36...”
PMN2A_0867 Aminotransferase class-III pyridoxal-phosphate:Acetylornithin... from Prochlorococcus marinus str. NATL2A
42% identity, 90% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...PMT1493 PMT0191 Prochlorococcus marinus MED 4 PMM1686 PMM1301, PMM1472 PMM0331 Prochlorococcus marinus NATL 2A PMN2A_1287 PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605 Syncc9605_1082, Syncc9605_2591 Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC 9902 Syncc9902_2230 Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH 8102 SYNW1412, SYNW2422 SYNW1634, SYNW0629 SYNW1956 Synechococcus...”
- “...PMT1493 PMT0191 Prochlorococcus marinus MED 4 n.d. PMM1301, PMM1472 PMM0331 Prochlorococcus marinus NATL 2A n.d. PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605 n.d. Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC 9902 n.d. Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH 8102 n.d. SYNW1634, SYNW0629 SYNW1956 Synechococcus sp. WH...”
HP15_3042 acetylornithine aminotransferase (EC 2.6.1.11); succinylornithine aminotransferase (EC 2.6.1.81) from Marinobacter adhaerens HP15
41% identity, 93% coverage
- mutant phenotype: Important for fitness in many minimal media experiments, and mostly rescued by addition of arginine, which suggests a role as acetylornithine aminotransferase. Important for utilization of arginine as a nitrogen source, which suggests a role as succinylornithine aminotransferase in the AST pathway. 65% identical to O30508 (AruC) from P. aeruginosa, which is reported to have these two activities.
Syncc9902_1534 Acetylornithine and succinylornithine aminotransferase from Synechococcus sp. CC9902
41% identity, 94% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...Synechococcus sp. CC 9605 Syncc9605_1082, Syncc9605_2591 Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC 9902 Syncc9902_2230 Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH 8102 SYNW1412, SYNW2422 SYNW1634, SYNW0629 SYNW1956 Synechococcus sp. WH 7805 WH7805_06086, WH7805_09974 WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416 Synechococcus sp. WH 5701 WH5701_03684, WH5701_03860 WH5701_07406, WH5701_15376 WH5701_06196...”
- “...PMN2A_1709 Synechococcus sp. CC 9605 n.d. Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC 9902 n.d. Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH 8102 n.d. SYNW1634, SYNW0629 SYNW1956 Synechococcus sp. WH 7805 n.d. WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416 Synechococcus sp. WH 5701 n.d. WH5701_07406, WH5701_15376 WH5701_06196 Synechococcus sp. RS...”
PSHAa0194 acetylornithine transaminase (NAcOATase and DapATase), PLP-dependent from Pseudoalteromonas haloplanktis TAC125
40% identity, 93% coverage
- Modelling microbial metabolic rewiring during growth in a complex medium
Fondi, BMC genomics 2016 - “...motif found upstream of its genes Cluster name Motif name Genes Weblogo Cluster 2 ArgR PSHAa0194, PSHAa0698,PSHAa2175, PSHAa2287, PSHAa2290, PSHAa2291, PSHAa2292, PSHAb0333, PSHAb0428, PSHAb0543 Cluster 3 CcpA PSHAa0189, PSHAa0609, PSHAa0740, PSHAa1167, PSHAa1648, PSHAa1649, PSHAa1650, PSHAa1651, PSHAa2167, PSHAb0082, PSHAb0345 Cluster 6 GalR PSHAa0603, PSHAa0871, PSHAa1364, PSHAa1767, PSHAa2301,...”
A8A0U0 Succinylornithine transaminase from Escherichia coli O9:H4 (strain HS)
43% identity, 89% coverage
A0A140N9B6 succinylornithine transaminase (EC 2.6.1.81) from Escherichia coli (see paper)
42% identity, 89% coverage
PSHAb0428 putative acetylornithine transaminase from Pseudoalteromonas haloplanktis TAC125
40% identity, 93% coverage
- Modelling microbial metabolic rewiring during growth in a complex medium
Fondi, BMC genomics 2016 - “...name Motif name Genes Weblogo Cluster 2 ArgR PSHAa0194, PSHAa0698,PSHAa2175, PSHAa2287, PSHAa2290, PSHAa2291, PSHAa2292, PSHAb0333, PSHAb0428, PSHAb0543 Cluster 3 CcpA PSHAa0189, PSHAa0609, PSHAa0740, PSHAa1167, PSHAa1648, PSHAa1649, PSHAa1650, PSHAa1651, PSHAa2167, PSHAb0082, PSHAb0345 Cluster 6 GalR PSHAa0603, PSHAa0871, PSHAa1364, PSHAa1767, PSHAa2301, PSHAb0295 Cluster 2. This cluster, as described...”
- Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125
Médigue, Genome research 2005 - “...Continued Proteins size Locus Against osmotic shock PSHAb0426 PSHAb0427 PSHAb0428 Helicases [DEA(DH)] PSHAa0109 Gene name Chr. 489 2 345 402 2 2 463 dbpA 1...”
4adbB / P77581 Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
42% identity, 91% coverage
- Ligand: pyridoxal-5'-phosphate (4adbB)
AstC / b1748 succinylornithine transaminase (EC 2.6.1.81; EC 2.6.1.11) from Escherichia coli K-12 substr. MG1655 (see 8 papers)
astC / P77581 succinylornithine transaminase (EC 2.6.1.81; EC 2.6.1.11) from Escherichia coli (strain K12) (see 6 papers)
ASTC_ECOLI / P77581 Succinylornithine transaminase; SOAT; Carbon starvation protein C; Succinylornithine aminotransferase; EC 2.6.1.81 from Escherichia coli (strain K12) (see paper)
astC / RF|NP_416262.1 succinylornithine transaminase; EC 2.6.1.81 from Escherichia coli K12 (see 5 papers)
A7ZML6 Succinylornithine transaminase from Escherichia coli O139:H28 (strain E24377A / ETEC)
b1748 succinylornithine transaminase, PLP-dependent from Escherichia coli str. K-12 substr. MG1655
42% identity, 89% coverage
- function: Catalyzes the transamination of N(2)-succinylornithine and alpha-ketoglutarate into N(2)-succinylglutamate semialdehyde and glutamate. Can also act as an acetylornithine aminotransferase. Is involved in the utilization of arginine as a nitrogen source, via the AST pathway, and seems also to play a role in ornithine catabolism (PubMed:9696779).
catalytic activity: N(2)-succinyl-L-ornithine + 2-oxoglutarate = N-succinyl-L- glutamate 5-semialdehyde + L-glutamate (RHEA:16953)
cofactor: pyridoxal 5'-phosphate
disruption phenotype: Cells lacking this gene lose the ability to grow on arginine as the nitrogen source. The disruption of this gene also impairs ornithine catabolism. - Peptidylarginine Deiminase Inhibitors Reduce Bacterial Membrane Vesicle Release and Sensitize Bacteria to Antibiotic Treatment
Kosgodage, Frontiers in cellular and infection microbiology 2019 - “...protein L13 rplM 99365 135.62 P0AA45 Ribosomal small subunit pseudouridine synthase A rsuA 50727 162.27 A7ZML6 Succinylornithine transaminase astC 232079 73.58 B7MPT2 Curved DNA binding protein cbpA 6123.2 90.68 P69911 Glutamate decarboxylase beta gadB 387378 72.46 A7ZY39 Cyclic pyranopterin monophosphate synthase accessory protein moaC 17232 67.03...”
- Toward Single Bacterium Proteomics
Végvári, Journal of the American Society for Mass Spectrometry 2023 - “...PdeC pdeC P32701 [3643] [K].SEVNNQLR.[T] 1189.64 595.33 +2 41.77 4 177.29 25.00% succinylornithine transaminase astC P77581 [353360] [K].QISQEAAK.[A] 1333.77 667.39 +2 45.17 1 189.61 6.25% elongation factor Tu2 tufB P0CE48 [178188] [K].ALEGDAEWEAK.[I] 1676.89 559.64 +3 63.80 10 127.64 50.00% 11 2-iminobutanoate/2-iminopropanoate deaminase ridA P0AF93 [5258] [R].QSLDNVK.[A]...”
- Knowns and Unknowns of Vitamin B6 Metabolism in Escherichia coli
Tramonti, EcoSal Plus 2021 (secret) - Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...family (P36839, P94427, P53555, P71084, P30949, P33189, O34662, P38021, P22256, P50457, P12995, P23893, P48247, P18335, P77581, O30508, Q9I606, Q9I6 M4, Q9I693, Q9I700, Q9I6J2, Q9I6R7, Q9HV04, Q9HTP1, Q9HWU0, Q9HT50, P48247, Q9I168, P12677, Q82 MM1, P21267, P40732, Q8ZPV2, Q8ZLX7), Thiamine Diphosphate Decarboxylase family (P96591, P37251, P23970, Q04789, P42415,...”
- Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure
LaVoie, BMC genomics 2018 - “...b1684 sufA Scaffold protein for assembly of iron-sulfur clusters 32 39 5 n.s. 3 n.s. b1748 astC Succinylornithine transaminase; carbon starvation protein 30 31 25 34 36 n.s. b4663 azuC Function unknown; membrane-associated 27 25 36 41 49 n.s. b0484 copA Copper-, silver-translocating P-type ATPase efflux...”
- “...n.s. b3686 ibpB Chaperone, heat-inducible protein of HSP20 family 402 124 281 37 211 9 b1748 astC Succinylornithine transaminase; carbon starvation protein 30 31 25 34 36 n.s. b3469 zntA Zn(II), Cd(II), and Pb(II) translocating P-type ATPase 40 19 8 29 22 n.s. b2582 trxC Thioredoxin...”
- Improved Metabolic Models for E. coli and Mycoplasma genitalium from GlobalFit, an Algorithm That Simultaneously Matches Growth and Non-Growth Data Sets
Hartleb, PLoS computational biology 2016 - “...PPS b2383 2x10 -22 2x10 -22 b3176 PGAMT b2048 3x10 -16 1x10 -18 b3359 SDPTA b1748 1x10 -180 1x10 -180 Solving FNp: Removing biomass components Removing metabolites from the biomass reaction can convert FNp to TPp, as all genes involved in the production (or, if the...”
- Genome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding
Bonocora, PLoS genetics 2015 - “...b1488 ddpX 34 10.511 P OS17 1830088 7 C TGGC ACGAACCC TGC A 1830087 - b1748 astC 62 11.294 C OS18 1864820 6 A TGGC ATGAGAG TTGC T 1864820 + b1783 yeaG 93 10.573 P OS19 2060018 24 C TGGC AAGCATC TTGC A 2060021 - b1988...”
- 18th Congress of the European Hematology Association, Stockholm, Sweden, June 13–16, 2013
, Haematologica 2013 - Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions
Orth, BMC systems biology 2012 - “...folP (b3177) none identified yrbG (b3196) none identified kdsC (b3198) none identified argD (b3359) astC (b1748) 1.00E-146 gabT (b2662) 3.00E-64 puuE (b1302) 3.00E-54 patA (b3073) 4.00E-52 hemL (b0154) 3.00E-32 cysG (b3368) none identified ilvE (b3770) pabC (b1096) 8.00E-8 dapF (b3809) none identified argC (b3958) none identified...”
- Promoter and regulon analysis of nitrogen assimilation factor, sigma54, reveal alternative strategy for E. coli MG1655 flagellar biosynthesis
Zhao, Nucleic acids research 2010 - “...b3686 ibpB g small heat shock protein Adaptations, atypical conditions 4.4 2.1 185 168 aaccTGGTAAATGGTTTGCTGTatat b1748 astC g succinylornithine transaminase, also has acetylornitine transaminase activity, PLP-dependent Amino acid biosynthesis: Arginine 3.1 2.1 88 71 tggcTGGCACGAACCCTGCAATctac b3421 rtcB g conserved hypothetical protein Unknown 2.1 2.1 54 37...”
- Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones
Lee, Applied and environmental microbiology 2009 - “...functionc b1469 b1482 b1739 b1628 b1744 b1745 b1746 b1747 b1748 b2000 b2097 b2210 b2465 b2661 b2662 b2663 b2724 b2873 b3012 b3049 b3175 b3230 b3311 b3426 b3447...”
- A semi-supervised method for predicting transcription factor-gene interactions in Escherichia coli
Ernst, PLoS computational biology 2008 - “...CRP b1498, ydeN 1 Yes Also implicated based on conserved motif analysis in [10] IHF b1748, astC 1 DNaseI footprinting evidence [58] Fis b3864, spf 1 ChIP-chip signal peak in promoter region that did not meet stringent threshold [25] FNR b1256, ompW 1 1 Yes LacZ...”
- More
ECs2454 acetylornithine delta-aminotransferase from Escherichia coli O157:H7 str. Sakai
Z2780 acetylornithine delta-aminotransferase from Escherichia coli O157:H7 EDL933
42% identity, 89% coverage
PMM1301 Acetylornithine and succinylornithine aminotransferases from Prochlorococcus marinus sp. MED4
41% identity, 90% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...PMT9312_1565 PMT9312_0337 Prochlorococcus marinus MIT 9313 PMT2214 PMT0331, PMT1493 PMT0191 Prochlorococcus marinus MED 4 PMM1686 PMM1301, PMM1472 PMM0331 Prochlorococcus marinus NATL 2A PMN2A_1287 PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605 Syncc9605_1082, Syncc9605_2591 Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC 9902 Syncc9902_2230 Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp....”
- “...PMT9312_1565 PMT9312_0337 Prochlorococcus marinus MIT 9313 n.d. PMT0331, PMT1493 PMT0191 Prochlorococcus marinus MED 4 n.d. PMM1301, PMM1472 PMM0331 Prochlorococcus marinus NATL 2A n.d. PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605 n.d. Syncc9605_0858, Syncc9605_2052, Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC 9902 n.d. Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH...”
An15g02360 uncharacterized protein from Aspergillus niger
39% identity, 79% coverage
WP_038545752 aspartate aminotransferase family protein from Synechococcus sp. KORDI-100
41% identity, 95% coverage
ACIAD1231 acetylornithine aminotransferase from Acinetobacter sp. ADP1
41% identity, 92% coverage
Q688Q8 acetylornithine transaminase from Oryza sativa subsp. japonica
40% identity, 81% coverage
SAUSA300_0187 ornithine--oxo-acid transaminase from Staphylococcus aureus subsp. aureus USA300_FPR3757
42% identity, 94% coverage
- CcpA regulates arginine biosynthesis in Staphylococcus aureus through repression of proline catabolism
Nuxoll, PLoS pathogens 2012 - “...2.3.1.35/2.3.1.1; argB (acetylglutamate kinase), SAUSA300_0184, EC 2.7.2.8; argC (N-acetyl-gamma-glutamyl-phosphate-reductase), SAUSA300_0186, EC 1.2.1.38; argD (acetylornithine transaminase), SAUSA300_0187, EC 2.6.1.11; argF (ornithine carbamoyl transferase), SAUSA300_1062, EC 2.1.3.3; argG (argininosuccinate synthase), SAUSA300_0864, EC 6.3.4.5; and argH (argininosuccinate lyase), SAUSA300_0863, EC 4.3.2.1. However, as previously reported and confirmed in this...”
SA0179 ornithine aminotransferase from Staphylococcus aureus subsp. aureus N315
41% identity, 94% coverage
Pchl3084_2275 aspartate aminotransferase family protein from Pseudomonas chlororaphis subsp. aureofaciens 30-84
41% identity, 92% coverage
BCAL2701 aminotransferase class-III from Burkholderia cenocepacia J2315
41% identity, 93% coverage
HMPREF0010_00392 aspartate aminotransferase family protein from Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841
41% identity, 91% coverage
RS9917_02041 Acetylornithine and succinylornithine aminotransferase from Synechococcus sp. RS9917
42% identity, 93% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...WH7805_06416 Synechococcus sp. WH 5701 WH5701_03684, WH5701_03860 WH5701_07406, WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 RS9917_06190 RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera watsonii WH 8501 n.d. CwatDRAFT_5161 CwatDRAFT_0865, CwatDRAFT_0842, CwatDRAFT_0969 Trichodesmium erythraeum IMS 101 TeryDRAFT_4567 TeryDRAFT_3251 TeryDRAFT_2672 TeryDRAFT_3296, TeryDRAFT_3923 Freshwater species Synechococcus elongatus sp. PCC 6301 n.d. Syc0599_c, Syc1466_c...”
- “...WH7805_13803 WH7805_06416 Synechococcus sp. WH 5701 n.d. WH5701_07406, WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 n.d. RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera watsonii WH 8501 CwatDRAFT_0830 CwatDRAFT_5161 CwatDRAFT_0865, CwatDRAFT_0842, CwatDRAFT_0969 Trichodesmium erythraeum IMS 101 TeryDRAFT_2282 TeryDRAFT_3251 TeryDRAFT_2672 TeryDRAFT_3296, TeryDRAFT_3923 Freshwater species Synechococcus elongatus sp. PCC 6301 n.d. Syc0599_c, Syc1466_c...”
BPSL0926 aminotransferase class-III from Burkholderia pseudomallei K96243
42% identity, 93% coverage
MSMEG_3773 acetylornithine aminotransferase from Mycobacterium smegmatis str. MC2 155
MSMEG_3773 acetylornithine transaminase from Mycolicibacterium smegmatis MC2 155
43% identity, 96% coverage
- Elucidating the role of c-di-AMP in Mycobacterium smegmatis: Phenotypic characterization and functional analysis
Chaudhary, Heliyon 2023 - “...argF (MSMEG_3772) Ornithine carbamoyltransferase 1.12 0.033 15 argB (MSMEG_3774) Acetylglutamate kinase 1.17 0.025 16 argD (MSMEG_3773) Acetylornithine aminotransferase 1.25 0.049 Table 2 Genes under several main categories are listed which are significantly downregulated in M. smegmatis pde strain compared to M. smegmatis WT along with their...”
- Multiform antimicrobial resistance from a metabolic mutation
Schrader, Science advances 2021 - “...the arginine biosynthesis pathway: one in argA ( MSMEG_2691 ) and eight in argD ( MSMEG_3773 ) ( Fig. 2, A and B , and table S2). In addition to a large increase in survival upon exposure to kanamycin, the mutants had increased survival when exposed...”
- Protein Composition of Mycobacterium smegmatis Differs Significantly Between Active Cells and Dormant Cells With Ovoid Morphology
Trutneva, Frontiers in microbiology 2018 - “...synthesis (MSMEG_0953, MSMEG_0956, and MSMEG_2780), leucine synthesis (MSMEG_6271 and MSMEG_2379) and arginine synthesis (MSMEG_3769, MSMEG_3770, MSMEG_3773, and MSMEG_3774). Dormant cells evidently accumulate storage materials: we found several enzymes responsible for the synthesis and accumulation of glycogen (MSMEG_4918), trehalose (MSMEG_6514 and MSMEG_6515 unique; Supplementary Table S2 )...”
NWMN_0129 ornithine aminotransferase from Staphylococcus aureus subsp. aureus str. Newman
41% identity, 94% coverage
SYNW1634 putative N-acetylornithine aminotransferase from Synechococcus sp. WH 8102
41% identity, 88% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...Syncc9605_0497 Synechococcus sp. CC 9902 Syncc9902_2230 Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH 8102 SYNW1412, SYNW2422 SYNW1634, SYNW0629 SYNW1956 Synechococcus sp. WH 7805 WH7805_06086, WH7805_09974 WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416 Synechococcus sp. WH 5701 WH5701_03684, WH5701_03860 WH5701_07406, WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 RS9917_06190 RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera...”
- “...Syncc9605_0659 Syncc9605_0497 Synechococcus sp. CC 9902 n.d. Syncc9902_1534, Syncc9902_0620 Syncc9902_1838 Synechococcus sp. WH 8102 n.d. SYNW1634, SYNW0629 SYNW1956 Synechococcus sp. WH 7805 n.d. WH7805_05656, WH7805_12388, WH7805_13803 WH7805_06416 Synechococcus sp. WH 5701 n.d. WH5701_07406, WH5701_15376 WH5701_06196 Synechococcus sp. RS 9917 n.d. RS9917_02041, RS9917_05240 RS9917_02641 Crocosphaera watsonii WH...”
AFUA_2G12470, Afu2g12470 acetylornithine aminotransferase from Aspergillus fumigatus Af293
38% identity, 77% coverage
- Arginine Auxotrophy Affects Siderophore Biosynthesis and Attenuates Virulence of Aspergillus fumigatus
Dietl, Genes 2020 - “...synthase/ornithine acetyltransferase; Arg2 (AFUA_2G11490), acetylglutamate synthase; ArgEF (AFUA_6G02910), bifunctional acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; Arg8 (AFUA_2G12470), acetylornithine aminotransferase; ArgB (AFUA_4G07190; in red), ornithine carbamoyltransferase; AmcA (AFUA_8G02760), mitochondrial ornithine exporter; AgaA (AFUA_3G11430), arginase; Cap1 (AFUA_5G06780), carbamoyl-phosphate synthase. Dashed lines indicate metabolic routes involving more than one enzyme....”
- Characterization of the Link between Ornithine, Arginine, Polyamine and Siderophore Metabolism in Aspergillus fumigatus
Beckmann, PloS one 2013 - “...of the three ornithine/arginine biosynthetic genes encoding acetylglutamate synthase (AFUA_2g11490), ArgEF (AFUA_6g02910) and acetylornithine aminotransferase (AFUA_2g12470) in A. fumigatus D141 ( Figure 6 ), as previously shown for strain ATCC 46645 [22] . CpcA-deficiency slightly upregulated the transcript levels of these genes during iron starvation (...”
- HapX-mediated adaption to iron starvation is crucial for virulence of Aspergillus fumigatus
Schrettl, PLoS pathogens 2010 - “...(B): 1, acetylglutamate synthetase (Afu2g11490) 2, acetylglutamate kinase and glutamate-5-semialdehyde dehydrogenase (Afu6g02910); 3, acetylornithine aminotransferase (Afu2g12470); 4, arginine biosynthesis bifunctional enzyme (Afu5g08120); 5, carbamoylphosphate synthase(Afu5g06780); 6, ornithine carbamoyltransferase (Afu4g07190); 7, arginase (Afu3g11430); 8, ornithine aminotransferase (Afu4g09140); 9, ornithine decarboxylase (Afu4g08010); 10, pyrroline carboxylate dehydrogenase (Afu6g08750); 11,...”
- The Aspergillus fumigatus transcriptional regulator AfYap1 represents the major regulator for defense against reactive oxygen intermediates but is dispensable for pathogenicity in an intranasal mouse infection model
Lessing, Eukaryotic cell 2007 - “...MetH/D (AFUA_4G07360)....................2.39 Aminotransferase (AFUA_2G12470) .........................................1.82 Peptidyl cis-trans isomerase...”
HMPREF0389_01570 aspartate aminotransferase family protein from Filifactor alocis ATCC 35896
41% identity, 94% coverage
- FACIN, a Double-Edged Sword of the Emerging Periodontal Pathogen Filifactor alocis: A Metabolic Enzyme Moonlighting as a Complement Inhibitor
Jusko, Journal of immunology (Baltimore, Md. : 1950) 2016 - “...available in www.ncbi.nlm.nih.gov/gene/ with following NCBI accession numbers HMPREF0389_01649 (encoding NAD-specific glutamate dehydrogenase, GluD) and HMPREF0389_01570 (encoding acetylornithine transaminase, FACIN) were amplified from genomic DNA by PCR. PCR products were purified and cloned into the pET-28a expression vector (Novagen) using NcoI/XhoI (GluD) or XbaI/XhoI (FACIN) restriction...”
- “...HMPREF0389_01649 NAD-specific glutamate dehydrogenase 794/1.94 40(33) 47231 3 HMPREF0389_00745 Acetyl-CoA acetyltransferase 196/0.47 9(5) 41067 4 HMPREF0389_01570 Acetylornithine aminotransferase 849/1.72 36(32) 44653 4 HMPREF0389_01649 NAD-specific glutamate dehydrogenase 145/0.22 4(4) 47231 5 HMPREF0389_00744 Butyryl-CoA dehydrogenase 665/1.15 18(17) 41459 5 HMPREF0389_00567 Glyceraldehyde-3-phosphate dehydrogenase 102/0.18 2(2) 37599 a Accession numbers...”
- Filifactor alocis--a new emerging periodontal pathogen
Aruni, Microbes and infection 2015 - “...in arginine metabolism and citrulline synthesis, such as arginine deiminase (HMPREF 0389_01584), acetyl ornithine transferase (HMPREF0389_01570) [ 20 ], aminotransferases (HMPREF0389_01352 and HMPREF0389_01353), amino-transferase family protein (HMPREF 0389_00349), arginine tRNA ligase (HMPREF0389_00390), and arginine decarboxylase (HMPREF0389_00102) ( http://www.ncbi.nlm.nih.gov/bioproject/46625 ), indicate that the nutritional needs of the...”
- “...have citrullination-induced systemic implications. It is also important to note that the proteins ornithine transaminase (HMPREF0389_01570), acetyl glutamate kinase (HMPREF0389_01569), glutamate racemase (HMPRE F0389_00100) and aminotransferase (HMPREF0389_00478) involved in ornithine biosynthesis were identified in F. alocis . In fact, arginine deiminase (HMPREF0389_01584) involved in ornithine catabolism...”
- Filifactor alocis: The Newly Discovered Kid on the Block with Special Talents
Aruni, Journal of dental research 2014 - “...(HMPREF 0389_01584), acetyl ornithine transferase (HMPREF0389_01570) (Aruni et al., 2012), aminotransferases (HMPREF0389_01352 and HMPREF0389_01353),...”
- “...to note that protein ornithine transaminase (HMPREF0389_01570), acetyl glutamate kinase (HMPREF0389_ 01569), glutamate racemase (HMPREF0389_ 00100), and...”
- Proteome analysis of coinfection of epithelial cells with Filifactor alocis and Porphyromonas gingivalis shows modulation of pathogen and host regulatory pathways
Aruni, Infection and immunity 2014 - “...(HMPREF0389_ 01584), acetyl ornithine transferase (HMPREF0389_01570) (36), aminotransferase (HMPREF0389_01352 and HMPREF0389_ 01353), amidotransferase family...”
- Proteome variation among Filifactor alocis strains
Aruni, Proteomics 2012 - “...proteases and peptidase. It is important to note that protein spots corresponding to ornithine transaminase (HMPREF0389_01570), acetyl glutamate kinase (HMPREF0389_01569), glutamate racemase (HMPREF0389_00100), and amidotransferase (HMPREF0389_00478) involved in ornithine biosynthesis were identified. These proteins were found both in the membrane and the extracellular fraction of the...”
- “...coA acetyl transferase 41/41.3 819/1.74 54 4.235 C 0.110M Thiolase domain 0.89 Nonsecretory protein 23. HMPREF0389_01570 Acetyl ornithine transaminase 43.9/44 550/1.22 52 0.203 C 0.432 CM AAT superfamily domain 0.89 Nonsecretory protein 24. HMPREF0389_00225 Transcriptional regulatory protein 48.3/47.9 64/0.09 5 0.128 CM No conserved domains 0.68...”
Q5JFW3 Putative [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
TK0275 N2-acetyl-lysine aminotransferase from Thermococcus kodakaraensis KOD1
41% identity, 98% coverage
- Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea
Pfeiffer, Genes 2021 - “...] 23434852 only for Arg, not for Lys biosynthesis 2a HVO_0043 (cont.) yes/no 42% TK0275 Q5JFW3 [ 125 ] 27566549 only for Arg, not for Lys biosynthesis 2a HVO_0042 argE no 36% TT_C1396 Q8VUS5 [ 124 ] [ 131 ] 19620981 28720495 for Arg, not for...”
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...TK0186 G6 (2-Aminoadipate transaminase) TK0250 G7 (Histidinol-phosphate aminotransferase) TK0864 G8 (Threonine- O -3-phosphate decarboxylase) II TK0275 LysW--L-lysine aminotransferase Yoshida et al. (2016) TK1211 Leu/Met racemase Leu, Met *** Zheng et al. (2021) TK2101 Ornithine -aminotransferase l -Orn, l -Lys, ( d -Orn, d -Lys) 2-OG, 2-oxoadipate,...”
- Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea
Pfeiffer, Genes 2021 - “...126 ] 23434852 only for Arg, not for Lys biosynthesis 2a HVO_0043 (cont.) yes/no 42% TK0275 Q5JFW3 [ 125 ] 27566549 only for Arg, not for Lys biosynthesis 2a HVO_0042 argE no 36% TT_C1396 Q8VUS5 [ 124 ] [ 131 ] 19620981 28720495 for Arg, not...”
- Lysine Biosynthesis of Thermococcus kodakarensis with the Capacity to Function as an Ornithine Biosynthetic System
Yoshida, The Journal of biological chemistry 2016 - “...lysW lysX TK0278 B lysY lysZ lysJ lysK TK0277 TK0276 TK0275 TK0274 COOH HOOC HOOC NH2 COOH NH2 glutamate -aminoadipate TK0278 LysW, ATP ADP ATP, LysW ADP TK0276...”
- “...ADP TK0277 NADPH NADP+ NADPH NADP+ TK0275 glutamate/AAA 2-oxoglutarate/2-oxoadipate glutamate/AAA 2-oxoglutarate/2-oxoadipate TK0274 LysW H2N COOH H2N NH2...”
Dgeo_1416 acetylornithine and succinylornithine aminotransferases from Deinococcus geothermalis DSM 11300
40% identity, 91% coverage
- Evolution of lysine biosynthesis in the phylum deinococcus-thermus
Nishida, International journal of evolutionary biology 2012 - “...Deinococcus radiodurans DR_1674 DR_0794 DR_1413 DR_1420 DR_0963 DR_2194 DR_1614 DR_1610 DR_1238 Deinococcus geothermalis Dgeo_2084 Dgeo_1458 Dgeo_1416 Dgeo_1391 Dgeo_0678 Dgeo_0685 Dgeo_1151* Dgeo_1154* Dgeo_1156* Dgeo_1257 Deinococcus deserti Deide_09240 Deide_16910 Deide_17960 Deide_10430 Deide_10350 Deide_13430* Deide_13460* Deide_13470* Deide_13980 Deinococcus maricopensis Deima_0046 Deima_1545 Deima_2454 Deima_2593 Deima_1346* Deima_1349* Deima_1350* Deima_1353* Deima_1355* Deima_1358*...”
lysJ / Q4JAP8 [amino group carrier protein]-C-terminal-L-glutamyl-γ-L-lysine aminotransferase (EC 2.6.1.118; EC 2.6.1.124) from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see 2 papers)
Saci_0755 acetylornithine aminotransferase from Sulfolobus acidocaldarius DSM 639
Q4JAP8 [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
39% identity, 97% coverage
- Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea
Pfeiffer, Genes 2021 - “...[ 130 ] 11489859 for Arg, not for Lys biosynthesis 2a HVO_0043 (cont.) yes/no 40% Saci_0755 Q4JAP8 [ 126 ] 23434852 only for Arg, not for Lys biosynthesis 2a HVO_0043 (cont.) yes/no 42% TK0275 Q5JFW3 [ 125 ] 27566549 only for Arg, not for Lys biosynthesis...”
- Early Response of Sulfolobus acidocaldarius to Nutrient Limitation
Bischof, Frontiers in microbiology 2018 - “...genes of the arginine biosynthesis pathway were decreased with the only exception of lysJ ( saci_0755 ) (Figure 6 and Supplementary Table S2 ). Further, transcript levels of argG ( saci_1617 , argininosuccinate synthase), argH (argininosuccinate lyase), speA ( saci_1363 , arginine decarboxylase), speB [ saci_0863...”
- Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea
Pfeiffer, Genes 2021 - “...130 ] 11489859 for Arg, not for Lys biosynthesis 2a HVO_0043 (cont.) yes/no 40% Saci_0755 Q4JAP8 [ 126 ] 23434852 only for Arg, not for Lys biosynthesis 2a HVO_0043 (cont.) yes/no 42% TK0275 Q5JFW3 [ 125 ] 27566549 only for Arg, not for Lys biosynthesis 2a...”
8ht4B / Q59282 Crystal structure of acetylornithine aminotransferase complex with plp from corynebacterium glutamicum (see paper)
44% identity, 95% coverage
- Ligand: pyridoxal-5'-phosphate (8ht4B)
cg1583 acetylornithine aminotransferase from Corynebacterium glutamicum ATCC 13032
NCgl1343 acetylornithine transaminase from Corynebacterium glutamicum ATCC 13032
44% identity, 95% coverage
- Acetohydroxyacid synthase, a novel target for improvement of L-lysine production by Corynebacterium glutamicum
Blombach, Applied and environmental microbiology 2009 - “...acid biosynthesis cg1290 cg1436 cg1437 cg1487 cg1580 cg1582 cg1583 cg1584 cg1739 426 BLOMBACH ET AL. ACKNOWLEDGMENTS We thank Lothar Eggeling for providing...”
- Citrate utilization by Corynebacterium glutamicum is controlled by the CitAB two-component system through positive regulation of the citrate transport genes citH and tctCBA
Brocker, Journal of bacteriology 2009 - “...cg1380 cg3226 cg3227 cg3308 cg0810 cg1628 cg1581 cg1582 cg1583 cg3026 cg3027 cg3028 cg3029 cg1156 cg1451 cg0576 cg2894 cg1364 cg2157 cg1151 cg1386 cg0653 cg1147...”
- Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis
Follmann, BMC genomics 2009 - “...kinase 0 1.84 n.d. 1.1 - -0.8 3.1 - 0.0 - - - ArgR 33 cg1583 v argD Acetylornithine aminotransferase 0 1.66 n.d. 1.9 0.9 0.2 - - - - - - ArgR 34 cg1584 v argF Ornithine carbamoyltransferase 0 1.75 -0.12 1.2 0.4 -0.2 -...”
- Adaptation of Corynebacterium glutamicum to ammonium limitation: a global analysis using transcriptome and proteome techniques
Silberbach, Applied and environmental microbiology 2005 - “...cg0303 cg0873 cg1129 cg1333 cg1334 cg1338 cg1580 cg1581 cg1582 cg1583 cg1584 cg1585 cg1586 cg1588 cg1697 cg2391 cg2900 codAb ureA ureB ureC ureE ureF ureG ureD...”
- High-yield production of L-serine through a novel identified exporter combined with synthetic pathway in Corynebacterium glutamicum
Zhang, Microbial cell factories 2020 - “...transporter periplasmic component NCgl1302 10.05 1.96 5.13 Aldo/keto reductase NCgl1344 286.87 55.96 5.12 Ornithine carbamoyltransferase NCgl1343 280.65 57.24 4.9 Acetylornithine aminotransferase NCgl0746 43.30 9.04 4.7 Hypothetical protein NCgl1342 134.70 29.07 4.63 Acetylglutamate kinase NCgl2946 672.93 155.87 4.31 Hypothetical protein NCgl1022 89.53 21.28 4.20 Cysteine sulfinate desulfinase...”
- Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum
Wu, Frontiers in bioengineering and biotechnology 2019 - “...), N-acetyl-gamma-glutamyl-phosphate reductase (encoded by argC / NCgl1340 ), acetylornithine aminotransferase (encoded by argD / NCgl1343 ), and ornithine acetyltransferase (encoded by argJ / NCgl1341 ). Additionally, L-ornithine can be converted to L-citrulline and L-arginine through the enzymatic activities of ornithine carbamoyltransferase (encoded by argF /...”
- Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis
Liu, Journal of bacteriology 2010 - “...Methanothermobacter, MTH52; Corynebacterium 1, NCgl1343; Corynebacterium 2, NCgl1058; Desulfitobacterium, Dhaf1761; Arabidopsis, AT4G33680; Syntrophobacter,...”
- Citrate utilization by Corynebacterium glutamicum is controlled by the CitAB two-component system through positive regulation of the citrate transport genes citH and tctCBA
Brocker, Journal of bacteriology 2009 - “...NCgl2817 NCgl2881 NCgl0676 NCgl1383 NCgl1341 NCgl1342 NCgl1343 NCgl2635 NCgl2636 NCgl2637 NCgl2638 NCgl0975 NCgl1235 NCgl0471 NCgl2523 NCgl1161 NCgl1892...”
- Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum
Marienhagen, Journal of bacteriology 2005 - “...II IV I, II III IV I, II III IV NCgl1343 NCgl1500 NCgl2020 NCgl2123 NCgl2227 NCgl2355 NCgl2491 NCgl2510 NCgl2515 NCgl2747 a b pdxR III III argD sufS hisC ilvE...”
- Adaptation of Corynebacterium glutamicum to ammonium limitation: a global analysis using transcriptome and proteome techniques
Silberbach, Applied and environmental microbiology 2005 - “...NCgl1132 NCgl1133 NCgl1137 NCgl1340 NCgl1341 NCgl1342 NCgl1343 NCgl1344 NCgl1345 NCgl1346 NCgl1347 NCgl1446 NCgl2098 NCgl2528 cg0104 cg0113 cg0114 cg0115...”
- Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine
Lange, Applied and environmental microbiology 2003 - “...carbamoyltransferase argF (C. glutamicum) e 2626 NCgl1343 Acelylornithine aminotransferase argD (C. glutamicum) e 2627 NCgl1342 Acetylglutamate kinase argB...”
Deide_16910 putative bifunctional protein: acetylornithine transaminase/acetyl-lysine aminotransferase from Deinococcus deserti VCD115
41% identity, 91% coverage
- Evolution of lysine biosynthesis in the phylum deinococcus-thermus
Nishida, International journal of evolutionary biology 2012 - “...Deinococcus geothermalis Dgeo_2084 Dgeo_1458 Dgeo_1416 Dgeo_1391 Dgeo_0678 Dgeo_0685 Dgeo_1151* Dgeo_1154* Dgeo_1156* Dgeo_1257 Deinococcus deserti Deide_09240 Deide_16910 Deide_17960 Deide_10430 Deide_10350 Deide_13430* Deide_13460* Deide_13470* Deide_13980 Deinococcus maricopensis Deima_0046 Deima_1545 Deima_2454 Deima_2593 Deima_1346* Deima_1349* Deima_1350* Deima_1353* Deima_1355* Deima_1358* Deinococcus proteolyticus Deipr_0213 Truepera radiovictrix Trad_2841 Trad_1401* Trad_1404* Trad_1399* Trad_1395* Trad_1392*...”
Cj0227 acetylornithine aminotransferase from Campylobacter jejuni subsp. jejuni NCTC 11168
38% identity, 95% coverage
- In vivo and in silico determination of essential genes of Campylobacter jejuni
Metris, BMC genomics 2011 - “...genes FBA only cj0024, cj0026c, cj0066c, cj0075c, cj0127c, cj0146c, cj0172c, cj0187c, cj0194, cj0196c, cj0197c, cj0205, cj0227, cj0237, cj0240c, cj0273, cj0274, cj0286c, cj0288c, cj0296c, cj0297c, cj0298c, cj0306c, cj0307, cj0321, cj0326, cj0332c, cj0360, cj0384c, cj0405, cj0432c, cj0433c, cj0434, cj0435, cj0437, cj0443, cj0453, cj0490, cj0541, cj0542, cj0559, cj0576, cj0580c,...”
- Identification of Campylobacter jejuni genes involved in its interaction with epithelial cells
Novik, Infection and immunity 2010 - “...(cj0342c [uvrA]), amino acid metabolism and transport (cj0227 [argD] and cj1097, a putative transmembrane transport protein), purine biosynthesis (cj1208, a...”
- Key role of Mfd in the development of fluoroquinolone resistance in Campylobacter jejuni
Han, PLoS pathogens 2008 - “...(NADPH) small subunit 0.0007 0.067622 1.74 NT Cj0123c putative tRNA-dihydrouridine synthase 0.0020 0.076881 1.87 2.1 Cj0227 argD, acetylornithine aminotransferase 0.0208 0.151677 1.69 NT Cj0283c cheW , chemotaxis protein 0.0125 0.130143 1.52 NT Cj0415 putative GMC oxidoreductase subunit 0.0191 0.148174 1.53 NT Cj0490- ald , putative aldehyde...”
- Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barré syndrome by high-throughput AFLP analysis
Godschalk, BMC microbiology 2006 - “...153984 154075 Cj0150c, probable aminotransferase 5 - - + + + + 86 212438 212383 Cj0227, argD, probable acetylornithine aminotransferase 6 + + + + + - 420 573124 572971 Cj0612c, cft, probable ferritin; Cj0613, pstS, possible periplasmic phosphate binding protein 7 - - + +...”
PAAG_06217 acetylornithine aminotransferase from Paracoccidioides lutzii Pb01
39% identity, 78% coverage
- Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
Bailão, PLoS neglected tropical diseases 2014 - “...Amino acid biosynthesis 2 PAAG_03138 alanine-glyoxylate aminotransferase 381.01 7.33 1.40 2.6.1.44 Amino acid metabolism 2 PAAG_06217 acetylornithine aminotransferase 313.28 11.25 1.72 2.6.1.11 Arginine biosynthesis 1 PAAG_06506 aspartate aminotransferase 253.11 6.50 1.42 2.6.1.1 Aspartate and glutamate metabolism 1 PAAG_06835 cystathionine gamma-lyase 257.79 7.00 *** 4.4.1.1 Cysteine biosynthesis...”
3nx3A / Q9PIR7 Crystal structure of acetylornithine aminotransferase (argd) from campylobacter jejuni
38% identity, 97% coverage
- Ligand: magnesium ion (3nx3A)
CJJ81176_0252 acetylornithine aminotransferase from Campylobacter jejuni subsp. jejuni 81-176
38% identity, 96% coverage
ECH_0886 acetylornithine/succinyldiaminopimelate aminotransferase from Ehrlichia chaffeensis str. Arkansas
ECH_RS03645 aspartate aminotransferase family protein from Ehrlichia chaffeensis str. Arkansas
39% identity, 97% coverage
- Proteome Analysis Revealed Changes in Protein Expression Patterns Caused by Mutations in Ehrlichia chaffeensis
Kondethimmanahalli, Frontiers in cellular and infection microbiology 2019 - “...(ECH_0734), malate dehydrogenase (ECH_0175), bifunctional ornithine acetyltransferase/N-acetylglutamate synthase (ECH_0676), ATP synthase subunit (ECH_1088), acetylornithine transaminase (ECH_0886), 5'-nucleotidase SurE (ECH_0791), and leucine-tRNA ligase (ECH_0794) were also upregulated in the ECH_0379 mutant ( Figure 5B ). Four hypothetical proteins (ECH_0697; ECH_0836; ECH_0010; ECH_0531) were also among the highly...”
- “...dehydrogenase 1.48 ECH_0676 ECH_RS02840 Ornithine acetyltransferase/N-acetylglutamate synthase 1.35 ECH_1088 ECH_RS04415 ATP synthase subunit B 1.46 ECH_0886 ECH_RS03645 Acetylornithine transaminase 1.36 ECH_0170 ECH_RS00715 variable length PCR target protein 2.04 ECH_0791 ECH_RS03285 5-nucleotidase SurE 1.29 ECH_0794 ECH_RS03300 LeucinetRNA ligase 1.35 ECH_0697 ECH_RS02910 Hypothetical protein 1.48 ECH_0836 ECH_RS03455 Hypothetical...”
- Penicillin-binding protein of Ehrlichia chaffeensis: cytokine induction through MyD88-dependent pathway
Rahman, The Journal of infectious diseases 2012 - “...aa) and the full-length diaminopimelate aminotransferase (DPA, Ech_0886) were amplified by polymerase chain reaction (PCR) using E. chaffeensis chromosomal DNA...”
- Proteome Analysis Revealed Changes in Protein Expression Patterns Caused by Mutations in Ehrlichia chaffeensis
Kondethimmanahalli, Frontiers in cellular and infection microbiology 2019 - “...1.48 ECH_0676 ECH_RS02840 Ornithine acetyltransferase/N-acetylglutamate synthase 1.35 ECH_1088 ECH_RS04415 ATP synthase subunit B 1.46 ECH_0886 ECH_RS03645 Acetylornithine transaminase 1.36 ECH_0170 ECH_RS00715 variable length PCR target protein 2.04 ECH_0791 ECH_RS03285 5-nucleotidase SurE 1.29 ECH_0794 ECH_RS03300 LeucinetRNA ligase 1.35 ECH_0697 ECH_RS02910 Hypothetical protein 1.48 ECH_0836 ECH_RS03455 Hypothetical protein...”
SXYL_00238 ornithine--oxo-acid transaminase from Staphylococcus xylosus
38% identity, 94% coverage
PODANS_3_9430 uncharacterized protein from Podospora anserina S mat+
40% identity, 82% coverage
- CLPP-Null Eukaryotes with Excess Heme Biosynthesis Show Reduced L-arginine Levels, Probably via CLPX-Mediated OAT Activation
Key, Biomolecules 2024 - “...PODANS_5_6860 (GSA 2,1-aminomutase), but also PODANS_7_10880 (ornithine delta-aminotransferase or OAT), the aminotransferases PODANS_6_1290, PODANS_7_5560, and PODANS_3_9430, according to the STRING Heidelberg webserver [ 24 ]. In mice, the closest HemL homolog is OAT, but it also has significant homology with PHYKPL (5-phosphohydroxy-L-lysine phospholyase), ETNPPL (ethanolamine-phosphate phospholyase),...”
ASTC_SALTY / Q8ZPV2 Succinylornithine transaminase; SOAT; Succinylornithine aminotransferase; EC 2.6.1.81 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
STM14_1582 aspartate aminotransferase family protein from Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
STM1303 succinylornithine transaminase, also has acetylornitine transaminase activity from Salmonella typhimurium LT2
42% identity, 89% coverage
- function: Catalyzes the transamination of N(2)-succinylornithine and alpha-ketoglutarate into N(2)-succinylglutamate semialdehyde and glutamate. Can also act as an acetylornithine aminotransferase.
catalytic activity: N(2)-succinyl-L-ornithine + 2-oxoglutarate = N-succinyl-L- glutamate 5-semialdehyde + L-glutamate (RHEA:16953)
cofactor: pyridoxal 5'-phosphate - Probing the ArcA regulon under aerobic/ROS conditions in Salmonella enterica serovar Typhimurium
Morales, BMC genomics 2013 - “...- 125 GTTAACCAGATGTTA 2.00E-06 L-lactate permease + 136 GTTAACTATTTGTTG 5.20E-06 - 173 GTTAATTTAATGAAA 1.90E-05 STM1303 STM14_1582 argD 2.63 1.11 2.95 - 40 GTTATTTATATGTTA 2.80E-05 bifunctional succinylornithine transaminase + 112 GTTTATGCAATGTTA 5.70E-05 A location of the binding sequence is in bp upstream of the translation start site...”
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...Q9I693, Q9I700, Q9I6J2, Q9I6R7, Q9HV04, Q9HTP1, Q9HWU0, Q9HT50, P48247, Q9I168, P12677, Q82 MM1, P21267, P40732, Q8ZPV2, Q8ZLX7), Thiamine Diphosphate Decarboxylase family (P96591, P37251, P23970, Q04789, P42415, P07003, P0AEP7, P08142, P00892, P00893, P17109, P0AFI0, Q9HTQ7, Q9I3L0, Q9HVA0, Q9I3S7, Q9HUI8, Q9HUR2, Q9I280, Q9I207, Q9HYA3, Q9HWK1, Q8ZQF0, Q8ZNE8, Q8ZR85,...”
- Use of a promiscuous, constitutively-active bacterial enhancer-binding protein to define the σ⁵⁴ (RpoN) regulon of Salmonella Typhimurium LT2
Samuels, BMC genomics 2013 - “...glnH 16 + 20.8 897079 897062 TGGCATGATTTTTTCATT STM1285 yeaG 4.1 + 21.5 1363884 1363867 TGGCATGAGAGTTGCTTT STM1303 astC 4.0 + 21.7 1382105 1382122 TGGCACGAATGCTGCAAT STM1690 pspA 23 + 20.3 1782486 1782469 TGGCACGCAAATTGTATT STM2355 argT 3.5 + 16.2 2466359 2466376 TGGCATAAGACCTGCATG STM2360 4.8 + 23.6 2472731 2472714 TGGCATGCCTTTTGCTTT...”
- Probing the ArcA regulon under aerobic/ROS conditions in Salmonella enterica serovar Typhimurium
Morales, BMC genomics 2013 - “...6.83 - 125 GTTAACCAGATGTTA 2.00E-06 L-lactate permease + 136 GTTAACTATTTGTTG 5.20E-06 - 173 GTTAATTTAATGAAA 1.90E-05 STM1303 STM14_1582 argD 2.63 1.11 2.95 - 40 GTTATTTATATGTTA 2.80E-05 bifunctional succinylornithine transaminase + 112 GTTTATGCAATGTTA 5.70E-05 A location of the binding sequence is in bp upstream of the translation start...”
- Global transcriptional analysis of dehydrated Salmonella enterica serovar Typhimurium
Gruzdev, Applied and environmental microbiology 2012 - “...argE (STM4120) argC (STM4121) argB (STM4122) argD (STM1303) argI (STM4469) (Continued on following page) 7868 aem.asm.org Applied and Environmental Microbiology...”
- Mass spectrometry-based quantitative proteomic analysis of Salmonella enterica serovar Enteritidis protein expression upon exposure to hydrogen peroxide
Kim, BMC microbiology 2010 - “...29% STM1296 Putative oxidoreductase ydjA 20.13 6.75 29% STM1302 Exonuclease III xthA 30.79 6.19 23% STM1303 Succinylornithine transaminase astC 43.72 6.13 34% STM1310 NAD synthetase nadE 30.57 5.36 27% STM1378 Pyruvate kinase I pykF 50.66 5.66 31% STM1431 Superoxide dismutase sodB 21.35 5.58 35% STM1544 PhoPQ-regulated...”
- “...dehydrogenase gapA 31 3% STM1296 Putative oxidoreductase ydjA -30 5% STM1302 Exonuclease III xthA 0% STM1303 Succinylornithine transaminase astC 41 7% STM1310 NAD synthetase nadE 9 1% STM1378 Pyruvate kinase I pykF 87 12% STM1431 Superoxide dismutase sodB 110 20% STM1544 PhoPQ-regulated protein pqaA 19 2%...”
t1182 succinylornithine transaminase from Salmonella enterica subsp. enterica serovar Typhi Ty2
42% identity, 89% coverage
WPa_0783 aspartate aminotransferase family protein from Wolbachia endosymbiont of Culex quinquefasciatus Pel
36% identity, 97% coverage
arg1 / RF|NP_588255.1 acetylornithine aminotransferase; EC 2.6.1.11 from Schizosaccharomyces pombe (see paper)
O74548 Probable acetylornithine aminotransferase, mitochondrial from Schizosaccharomyces pombe (strain 972 / ATCC 24843)
SPCC777.09c acetylornithine aminotransferase from Schizosaccharomyces pombe
38% identity, 86% coverage
- CharProtDB Source (per GeneDB): GeneDB_Spombe
- Proteasome regulation of petite-negativity in fission yeast.
Amberg, bioRxiv : the preprint server for biology 2024 - “...Q01475 sar1 0.33 0.047 ADP-ribosylation factor Sar1 O59816 lat1 0.33 0.014 dihydrolipoamide S-acetyltransferase E2, Lat1 O74548 arg1 0.32 0.020 acetylornithine aminotransferase O13844 SPAC19G12.05 0.32 0.041 mitochondrial carrier, citrate Q9UU76 puf6 0.31 0.024 pumilio family RNA-binding protein Puf6 Q09794 ura1 0.31 0.011 carbamoyl-phosphate synthase (glutamine hydrolyzing), aspartate...”
- Thiol-independent action of mitochondrial thioredoxin to support the urea cycle of arginine biosynthesis in Schizosaccharomyces pombe
Song, Eukaryotic cell 2008 - “...parentheses (arg6, SPBC725.14; arg11, SPAC4G9.09c; arg1, SPCC777.09c; arg3, and SPAC4G9.10; arg12, SPBC428.05c; arg7, SPBC1773.14; car1, SPBP26C9.02c). (B)...”
- mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG
Soleimanpour-Lichaei, Molecular cell 2007 - “...and analyzed by Southern blotting with either the 1.3 kb arg1Sp nuclear gene ORF (accession SPCC777.09c) or the pDG3 plasmid containing the full S.pombe mitochondrial DNA cloned on pBR322 as described in Chiron etal. (2005) . De Novo Mitochondrial Protein Synthesis Protein translation in human cells...”
- NOVEL AUXOTROPHIC FISSION YEAST
KUNO, 2006
MAP_RS06930 acetylornithine transaminase from Mycobacterium avium subsp. paratuberculosis K-10
43% identity, 91% coverage
PMT9312_1397 acetylornithine and succinylornithine aminotransferases from Prochlorococcus marinus str. MIT 9312
40% identity, 90% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...Pro0374 Prochlorococcus marinus str. MIT 9211 P9211_09067 P9211_02002, P9211_10217 P9211_07012 Prochlorococcus marinus MIT 9312 PMT9312_1779 PMT9312_1397, PMT9312_1565 PMT9312_0337 Prochlorococcus marinus MIT 9313 PMT2214 PMT0331, PMT1493 PMT0191 Prochlorococcus marinus MED 4 PMM1686 PMM1301, PMM1472 PMM0331 Prochlorococcus marinus NATL 2A PMN2A_1287 PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605...”
- “...Pro0374 Prochlorococcus marinus str. MIT 9211 n.d. P9211_02002, P9211_10217 P9211_07012 Prochlorococcus marinus MIT 9312 n.d. PMT9312_1397, PMT9312_1565 PMT9312_0337 Prochlorococcus marinus MIT 9313 n.d. PMT0331, PMT1493 PMT0191 Prochlorococcus marinus MED 4 n.d. PMM1301, PMM1472 PMM0331 Prochlorococcus marinus NATL 2A n.d. PMN2A_0867, PMN2A_1003 PMN2A_1709 Synechococcus sp. CC 9605...”
glr3849 4-aminobutyrate aminotransferase from Gloeobacter violaceus PCC 7421
37% identity, 85% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...sp. PCC 6803 Sll1077, Sll0228 Slr1022 Sll1561, Slr0370, Slr0091 Gloeobacter violaceus PCC 7421 n.d. Glr0547, Glr3849, Gll2223 Glr2755, Glr3848, Gll1504, Gll2805 Nostoc sp. PCC 7120 Alr2310 Alr2398, Alr1080, All0396 Alr0540, Alr3771, All3556, All5022 Nostoc punctiforme PCC 73102 Npun02002114 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena...”
- “...Synechocystis sp. PCC 6803 Sll1336 Slr1022 Sll1561, Slr0370, Slr0091 Gloeobacter violaceus PCC 7421 Glr1758 Glr0547, Glr3849, Gll2223 Glr2755, Glr3848, Gll1504, Gll2805 Nostoc sp. PCC 7120 Alr4495 Alr2398, Alr1080, All0396 Alr0540, Alr3771, All3556, All5022 Nostoc punctiforme PCC 73102 Npun02001803 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena...”
BCG_1694 putative Acetylornithine aminotransferase argD from Mycobacterium bovis BCG str. Pasteur 1173P2
NP_216171 acetylornithine aminotransferase from Mycobacterium tuberculosis H37Rv
Rv1655 acetylornithine aminotransferase from Mycobacterium tuberculosis H37Rv
43% identity, 93% coverage
- Comparison of the transcriptome, lipidome, and c-di-GMP production between BCGΔBCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages
Flores-Valdez, Scientific reports 2024 - “...Rv2995c 3-isopropylmalate dehydrogenase leuB 0.636 0.02535765 BCG_1657 BCG_1657 Rv1619 Conserved membrane protein 0.6359 0.029269187 argD BCG_1694 Rv1655 Probable Acetylornithine aminotransferase argD 0.635 0.010948399 ArgR regulon fadD33 BCG_1407 Rv1345 Possible polyketide synthase fadD33 0.6338 0.007716787 BCG_1724c BCG_1724c Rv1686c Probable conserved integral membrane protein ABC transporter 0.6334 0.03708873...”
- InbR, a TetR family regulator, binds with isoniazid and influences multidrug resistance in Mycobacterium bovis BCG
Yang, Scientific reports 2015 - “...15 BCG_1692 2.18 Rv1653 argJ bifunctional ornithine acetyltransferase/N-acetylglutamate synthase BCG_1693 2.34 Rv1654 argB acetylglutamate kinase BCG_1694 2.34 Rv1655 argD acetylornithine aminotransferase NA BCG_1695 1.32 Rv1656 argF ornithine carbamoyltransferase BCG_1696 1.13 Rv1657 argR arginine repressor 16 BCG_2047c 1.94 2.94 Rv2028c hypothetical protein NA BCG_2048c 1.84 2.64 Rv2029c...”
- ArgD of Mycobacterium tuberculosis is a functional N-acetylornithine aminotransferase with moonlighting function as an effective immune modulator.
Nehvi, International journal of medical microbiology : IJMM 2022 (PubMed)- GeneRIF: ArgD of Mycobacterium tuberculosis is a functional N-acetylornithine aminotransferase with moonlighting function as an effective immune modulator.
- Comparison of the transcriptome, lipidome, and c-di-GMP production between BCGΔBCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages
Flores-Valdez, Scientific reports 2024 - “...3-isopropylmalate dehydrogenase leuB 0.636 0.02535765 BCG_1657 BCG_1657 Rv1619 Conserved membrane protein 0.6359 0.029269187 argD BCG_1694 Rv1655 Probable Acetylornithine aminotransferase argD 0.635 0.010948399 ArgR regulon fadD33 BCG_1407 Rv1345 Possible polyketide synthase fadD33 0.6338 0.007716787 BCG_1724c BCG_1724c Rv1686c Probable conserved integral membrane protein ABC transporter 0.6334 0.03708873 BCG_2589...”
- The efflux pumps Rv1877 and Rv0191 play differential roles in the protection of Mycobacterium tuberculosis against chemical stress
Sao, Frontiers in microbiology 2024 - “...only in the rv1878 mutant. These were rv1652 ( argC ), rv1653 ( argJ ), rv1655 ( argD ) which are all involved in the biosynthesis of arginine from glutamate ( Tiwari et al., 2018 ) and rv0858c encoding N-succinyldiaminopimelate aminotransferase (DapC; Weyand et al., 2006...”
- ArgD of Mycobacterium tuberculosis is a functional N-acetylornithine aminotransferase with moonlighting function as an effective immune modulator
Nehvi, International journal of medical microbiology : IJMM 2022 (PubMed)- “...an essential enzyme acetyl ornithine aminotransferase ArgD (Rv1655) of arginine biosynthetic pathway which plays crucial role in M. tuberculosis growth and...”
- “...India: LE Tracking ElsevierWaived http://creativecommons.org/licenses/by/4.0/ Rv1655 Arginine metabolism Drug target Macrophage activation NF B signaling...”
- Transcriptional regulation and drug resistance in Mycobacterium tuberculosis
Miotto, Frontiers in cellular and infection microbiology 2022 - “...( Schrader etal., 2021 ). These mutations mapping in the rv2747 ( argA ) and rv1655 ( argD ) genes were associated with increased survival during RIF exposure, as well as with minimum inhibitory concentration (MIC) increase to clarithromycin, again related to the up-regulation of whiB7...”
- Quantitative proteomics reveals that dormancy-related proteins mediate the attenuation in mycobacterium strains
Wang, Virulence 2021 - “...were significantly upregulated in the BCG strain, namely, ArgC (Rv1652), ArgJ (Rv1653), ArgB (Rv1654), ArgD (Rv1655), ArgF (Rv1656), ArgR (Rv1657), ArgG (Rv1658), and ArgH (Rv1659), which may indicate that the H37Rv and H37Ra strains experience a deficiency in arginine during the stationary phase. The glyoxylate cycle...”
- Multiform antimicrobial resistance from a metabolic mutation
Schrader, Science advances 2021 - “...would confer HS upon exposure to aminoglycosides in Mtb , we constructed an argD ( rv1655 ) Mtb mutant (fig. S6A). We chose to construct the argD mutant since we had obtained a larger diversity of mutations in this gene in Msm compared to argA ,...”
- “...cassette between sequences homologous to the ~500-bp regions upstream and downstream of the argD gene (Rv1655). Mtb H37Rv cells were first transformed with an episomal plasmid containing the phage-derived RecET recombinase (pNit-recET-sacB-kanR) ( 81 ) and grown to mid-log phase in 7H9 supplemented with kanamycin (25...”
- Comprehensive analysis of protein acetyltransferases of human pathogen Mycobacterium tuberculosis
Xie, Bioscience reports 2019 - “...mycobacteria, even Rhodococcus and Streptomyces. For example, the adjacent genes of Rv1653c including Rv1652, Rv1654, Rv1655, and Rv1656 are highly conserved in M. leprae TN, M. smegmatis str. mc 2 155, M. avium 104, Rhodococcus sp.RHA1, and Streptomyces avermitilis MA-4680 (Supplementary Figure S2). However, the adjacent...”
- Trehalose-6-Phosphate-Mediated Toxicity Determines Essentiality of OtsB2 in Mycobacterium tuberculosis In Vitro and in Mice
Korte, PLoS pathogens 2016 - “...Transformed p-value Upregulated Rv1258c Rv1258c 27.12 0.00010 Rv0662c vapB7 23.29 0.00005 Rv1257c Rv1257c 19.22 0.00025 Rv1655 argD 15.99 0.00018 Rv2164c Rv2164c 15.92 0.00041 Rv1652 argC 15.72 0.00006 Rv2165c Rv2165c 15.54 0.00230 Rv1654 argB 14.48 0.00017 Rv1656 argF 13.38 0.00001 Rv1987 Rv1987 12.46 0.00009 Downregulated Rv2628 Rv2628...”
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SLUN_RS38465 acetylornithine transaminase from Streptomyces lunaelactis
44% identity, 90% coverage
- Lunaemycins, New Cyclic Hexapeptide Antibiotics from the Cave Moonmilk-Dweller Streptomyces lunaelactis MM109T
Martinet, International journal of molecular sciences 2023 - “...StenM, Figure 1 , Table 1 ), and lun12 for the N 2 -acetyl-L-ornithine:2-oxoglutarate aminotransferase (SLUN_RS38465 homologous to StenJ, Figure 1 , Table 1 ). BGC 28a thus contains all genes required for the enzymatic conversion of glutamic acid and glutamine to L-Piz, further supporting the...”
- “...Lun11 342 StenM, N-acetyl-gamma-glutamyl-phosphate reductase (ArgC), stenothricin BGC from Streptomyces roseosporus (EFE73305.1) 81/100 L-ornithine synthesis SLUN_RS38465 WP_108155197.1 Lun12 417 StenJ, N(2)-acetyl-L-ornithine:2-oxoglutarate aminotransferase (ArgD), stenothricin BGC from Streptomyces roseosporus NRRL 15998 (EFE73302.1) 72/94 L-ornithine synthesis SLUN_RS38470 WP_108155198.1 Lun13 300 StenK, N-acetylglutamate kinase (ArgB), stenothricin BGC from Streptomyces...”
7nn4A / P9WPZ7 Crystal structure of mycobacterium tuberculosis argd with prosthetic group pyridoxal 5'-phosphate and 3-hydroxy-2-naphthoic acid.
43% identity, 95% coverage
- Ligand: 3-hydroxynaphthalene-2-carboxylic acid (7nn4A)
DR0794, DR_0794 acetylornithine aminotransferase from Deinococcus radiodurans R1
39% identity, 80% coverage
- Evolution of lysine biosynthesis in the phylum deinococcus-thermus
Nishida, International journal of evolutionary biology 2012 - “...Ocepr_1779* Marinithermus hydrothermalis Marky_1533 Marky_0665* Marky_0663* Marky_0666* Marky_0667* Marky_0668* Marky_0671* Marky_0672* Marky_0673* Deinococcus radiodurans DR_1674 DR_0794 DR_1413 DR_1420 DR_0963 DR_2194 DR_1614 DR_1610 DR_1238 Deinococcus geothermalis Dgeo_2084 Dgeo_1458 Dgeo_1416 Dgeo_1391 Dgeo_0678 Dgeo_0685 Dgeo_1151* Dgeo_1154* Dgeo_1156* Dgeo_1257 Deinococcus deserti Deide_09240 Deide_16910 Deide_17960 Deide_10430 Deide_10350 Deide_13430* Deide_13460* Deide_13470* Deide_13980...”
- Functional and evolutionary relationship between arginine biosynthesis and prokaryotic lysine biosynthesis through alpha-aminoadipate
Miyazaki, Journal of bacteriology 2001 - “...LysJ showed considerable identity to that of the DR0794 gene product from Deinococcus radiodurans (61% identity) (36), which is consistent with the...”
- “...analyzed, LysJ was found to be closely related to DR0794 of D. radiodurans (61% identity), which is a bacterium closely related taxonomically to T. thermophilus...”
SA0818 ornithine--oxo-acid transaminase from Staphylococcus aureus subsp. aureus N315
Q6GAW9 Ornithine aminotransferase 2 from Staphylococcus aureus (strain MSSA476)
SAV0957 ornithine--oxo-acid transaminase from Staphylococcus aureus subsp. aureus Mu50
SAOUHSC_00894 ornithine aminotransferase, putative from Staphylococcus aureus subsp. aureus NCTC 8325
SAUSA300_0860 Ornithine aminotransferase from Staphylococcus aureus subsp. aureus USA300_FPR3757
SACOL0960 ornithine aminotransferase from Staphylococcus aureus subsp. aureus COL
CH51_RS04860 ornithine--oxo-acid transaminase from Staphylococcus aureus
37% identity, 94% coverage
- Exposure of clinical MRSA heterogeneous strains to β-lactams redirects metabolism to optimize energy production through the TCA cycle
Keaton, PloS one 2013 - “...SA2318 8.4 hypothetical protein Similar to L-serine dehydratase (catalyses deamination of serine to form pyruvate). SA0818 rocD 4.2 ornithine-oxo-acid transaminase Involved in urea cycle and metabolism of amino groups. SA2341 rocA 4.8 1-pyrroline-5-carboxylate dehydrogenase Involved in L-proline degradation into L-glutamate SA1585 3 hypothetical protein Similar to...”
- Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
Anderson, FEMS immunology and medical microbiology 2010 - “...6.2 2.5 2.5 prfA SA2110 peptide chain release factor 1 sa_c8340s7313_at 3.4 2.5 2.5 prfB SA0818 peptide chain release factor 2 sa_c10084s8804_a_at 5.4 5 5 queA SA1695 S-adenosylmethionine:tRNA ribosyltransferase-isomerase sa_c1398s1173_at 3.4 2.5 2.5 rbfA SA1289 ribosome-binding factor A sa_c1316s1090_a_at * 3.8 2.5 2.5 rbgA SA1260 ribosomal...”
- Transcriptional signature following inhibition of early-stage cell wall biosynthesis in Staphylococcus aureus
O'Neill, Antimicrobial agents and chemotherapy 2009 - “...SA0422 SA0430 SA0431 SA0480 SA0481 SA0482 SA0612 SA0707 SA0818 SA0835 SA0845 SA0846 SA0847 SA0848 SA0849 SA0871 SA0883 SA0886 SA0958 SA1019 SA1163 SA1164 SA1165...”
- Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus
Donat, Journal of bacteriology 2009 - “...molecules SA0008 hutH Histidine ammonia lyase SA0818 rocD Ornithine aminotransferase SA0819 gudB NAD-specific glutamate dehydrogenase SA1348 bfmBAA...”
- “...prsA rocA rocD vraS SA0922 SA0925 SA0909 SA1691 SA0252 SA1659 SA2341 SA0818 SA1701 3.0 1.8 2.3 2.5 2.8 2.9 3.5 2.5 2.5 3.0 2.0 3.5 4.0 3.0 3.4 9.0 3.0 10.0 a...”
- Very low ethanol concentrations affect the viability and growth recovery in post-stationary-phase Staphylococcus aureus populations
Chatterjee, Applied and environmental microbiology 2006 - “...glutamate: ornithine by the ornithine aminotransferase (SA0818) and the 1-pyrroline-5-carboxylate dehydrogenase (SA2341) and proline by the proline...”
- Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci
Dunman, Journal of bacteriology 2001 - “...system IIB components SA2336 SA2425 SA0819 SA0818 SA1585 Conserved hypothetical Conserved hypothetical Conserved hypothetical Conserved hypothetical Conserved...”
- Multi-Omics Analysis Reveals Anti-Staphylococcus aureus Activity of Actinomycin D Originating from Streptomyces parvulus
Lin, International journal of molecular sciences 2021 - “...(Q8NXF3), 4,4-diaponeurosporen-aldehyde dehydrogenase (Q2FWX9), aminomethyltransferase (Q6GGG20), glycine cleavage system H protein (Q6GII3), ornithine aminotransferase 2 (Q6GAW9), 2-oxoglutarate dehydrogenase E1 component (Q6GGZ5), dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Q6GGZ6), formimidoylglutamase (Q6GEA1), and urocanate hydratase (Q6GEA4) were down-regulated, and all the proteins related to proline metabolism were...”
- The SAV1322 gene from Staphylococcus aureus: genomic and proteomic approaches to identification and characterization of gene function
Kim, BMC microbiology 2016 - “...A039 gi|15923948 NAD-specific glutamate dehydrogenase 970 gudB SAV0958 Down A046 gi|15923947 Ornithine--oxo-acid transaminase 1027 rocD SAV0957 Down A045 gi|15925103 Serine hydroxymethyltransferase 365 glyA SAV2113 Down A049 gi|15924192 Carbamoyl phosphate synthase small subunit 923 pyrAA SAV1202 Up A051 gi|15924159 Ornithine carbamoyltransferase 556 argF SAV1169 Up A060 gi|15924160...”
- Two novel point mutations in clinical Staphylococcus aureus reduce linezolid susceptibility and switch on the stringent response to promote persistent infection
Gao, PLoS pathogens 2010 - “...valine, leucine and isoleucine biosynthesis enzymes showed increased expression, as did genes such as rocD (SAV0957) and argJ (SAV0183) linked to ornithine and arginine production. Striking too was the up-regulation of the Opp3 oligopeptide transport system (SAV0986SAV0994). Opp3 facilitates the acquisition 48 aa-long peptides from the...”
- Microbial Metabolic Genes Crucial for S. aureus Biofilms: An Insight From Re-analysis of Publicly Available Microarray Datasets
Nassar, Frontiers in microbiology 2020 - “...sao00910 Nitrogen metabolism (KEGG) sao01100 Metabolic pathways (KEGG) sao01120 Microbial metabolism in diverse environments (KEGG) SAOUHSC_00894 rocD Upregulated Upregulated GO:0006561 proline biosynthetic process GO:0055129 L-proline biosynthetic process GO:0008652 cellular amino acid biosynthetic process sao00330 Arginine and proline metabolism (KEGG) sao01100 Metabolic pathways (KEGG) sao01110 Biosynthesis of...”
- Proteomic and Metabolomic Analyses of a Tea-Tree Oil-Selected Staphylococcus aureus Small Colony Variant
Torres, Antibiotics (Basel, Switzerland) 2019 - “...flavodoxin uncharacterized 2.5 SAOUHSC_00833 nitroreductase uncharacterized 1.9 SAOUHSC_00850 SUF system FeS assembly protein uncharacterized 1.6 SAOUHSC_00894 RocD ornithine-oxo-acid transaminase amino acid metabolism 1.8 SAOUHSC_00921 FabF 3-Oxoacyl-synthase lipid metabolism 1.8 SAOUHSC_00942 RelQ GTP pyrophosphokinase nucleotide metabolism 1.4 SAOUHSC_01178 CoaBC bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate-cysteine ligase cofactors and secondary metabolites...”
- The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus
Rochat, Nucleic acids research 2018 - “...11.9 SAOUHSC_00875 ndh2 Pyridine nucleotide-disulfide oxidoreductase 11.9 SAOUHSC_01017 purH Phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase 10.9 SAOUHSC_00894 rocD Ornithine aminotransferase 10.7 SAOUHSC_00819 cspC Cold shock domain protein 10.4 Full results are available under GEO accession number GSE106327. Figure 3. Combining experiments to uncover RsaE-targets. Venn diagram showing...”
- Increased mutability of Staphylococci in biofilms as a consequence of oxidative stress
Ryder, PloS one 2012 - “...Biofilms/Planktonic SAOUHSC_00502 ctsR Transcriptional regulator CtsR 2.20 up SAOUHSC_00526 Putative ribosomal protein L7Ae-like 2.30 up SAOUHSC_00894 rocD2 Ornithine aminotransferase 2 2.61 up SAOUHSC_00895 Glutamate dehydrogenase, NAD-specific, putative 2.36 up SAOUHSC_01002 qoxA Probable quinol oxidase subunit 2 2.00 up SAOUHSC_01042 Dihydrolipoamide S-acetyltransferase component of pyruvate dehydrogenase complex...”
- Human Urine Alters Methicillin-Resistant Staphylococcus aureus Virulence and Transcriptome
Paudel, Applied and environmental microbiology 2021 (secret) - A Novel Mechanism of Inactivating Antibacterial Nitro Compounds in the Human Pathogen Staphylococcus aureus by Overexpression of a NADH-Dependent Flavin Nitroreductase
El-Hossary, Antimicrobial agents and chemotherapy 2018 - “...SAUSA300_1939 SAUSA300_1930 SAUSA300_1943 SAUSA300_1936 SAUSA300_0860 SAUSA300_01030 SAUSA300_0117 SAUSA300_1715 SAUSA300_1713 SAUSA300_1714 SAUSA300_0504...”
- Heparin Mimics Extracellular DNA in Binding to Cell Surface-Localized Proteins and Promoting Staphylococcus aureus Biofilm Formation
Mishra, mSphere 2017 - “...SAUSA300_1367 Cytidylate kinase 3 3 16 None SAUSA300_1696 d -Alanine aminotransferase 6 5 22 None SAUSA300_0860 Ornithine aminotransferase 6 6 21 None SAUSA300_1880 Glutamyl-tRNA amidotransferase 6 6 15 None SAUSA300_0234 Putative flavohemoprotein 11 7 25 None SAUSA300_0135 Superoxide dismutase 6 9 43 None SAUSA300_2067 Serine hydroxymethyltransferase...”
- In vitro and in vivo models of Staphylococcus aureus endophthalmitis implicate specific nutrients in ocular infection
Sadaka, PloS one 2014 - “...antiterminator, BglGfamily 28.3 (1.4) 28.3 (1.6) ig_SACOL0913 Intergenic region downstream ofSACOL0913 15.2 (1.9) 6.3 (2.4) SACOL0960 rocD2 Ornithine aminotransferase 10.0 (2.0) 3.5 (1.2) SACOL1360 Aspartate kinase 10.6 (5.9) 6.0 (1.3) SACOL1734 gapA2 Glyceraldehyde-3-phosphate dehydrogenase 10.4 (4.9) 6.8 (4.9) SACOL1784 acuA Acetoin utilization protein AcuA 13.7 (3.6)...”
- Staphylococcus aureus PhoU Homologs Regulate Persister Formation and Virulence
Shang, Frontiers in microbiology 2020 - “...2.03 ND CH51_RS12955 Biotin transporter BioY 6.42 ND Proline metabolism CH51_RS00845 Acetylglutamate kinase 2.01 ND CH51_RS04860 Ornithine aminotransferase 2 2.15 ND CH51_RS16020 Hypothetical protein 7.01 ND CH51_RS08555 Pyrroline-5-carboxylate reductase 2.49 ND Lysine biosynthesis CH51_RS07635 4-Hydroxy-tetrahydrodipicolinate reductase 2.12 ND CH51_RS07655 Diaminopimelate decarboxylase 2.37 ND CH51_RS13980 DUF2338 domain-containing...”
HVO_0043 acetylornithine aminotransferase from Haloferax volcanii DS2
40% identity, 95% coverage
- Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea
Pfeiffer, Genes 2021 - “...46% TK0277 Q5JFW1 [ 125 ] 27566549 only for Arg, not for Lys biosynthesis 2a HVO_0043 argD no 45% TT_C1393 Q93R93 [ 130 ] 11489859 for Arg, not for Lys biosynthesis 2a HVO_0043 (cont.) yes/no 40% Saci_0755 Q4JAP8 [ 126 ] 23434852 only for Arg, not...”
- Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery
Kiljunen, BMC biology 2014 - “...argC N-acetyl-gamma-glutamyl-phosphate reductase H295/12-27 Arginine 44,905; - HVO_0045, argC N-acetyl-gamma-glutamyl-phosphate reductase H295/10-36 Arginine 43,144; + HVO_0043, argD Acetylornithine aminotransferase H295/17-48 Arginine 2,374,426; - HVO_2508, carA Carbamoyl-phosphate synthase small subunit H295/13-54 Histidine 1,198,248; + HVO_0431 HAD-superfamily hydrolase H295/12-18 Histidine 1,198,248; + HVO_0431 HAD-superfamily hydrolase H295/21-77 Proline 786,914;...”
- Genetic and proteomic analyses of a proteasome-activating nucleotidase A mutant of the haloarchaeon Haloferax volcanii
Kirkland, Journal of bacteriology 2008 - “...protein HVO_0043 .........................................................Aminotransferase (gabT, argD)...”
A0A0E1X760 Ornithine aminotransferase from Staphylococcus aureus subsp. aureus MN8
37% identity, 92% coverage
DA471_RS02540 ornithine--oxo-acid transaminase from Staphylococcus aureus
37% identity, 94% coverage
PAS_chr4_0645 Acetylornithine aminotransferase from Komagataella phaffii GS115
38% identity, 87% coverage
- Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
Li, Genes 2019 - “...enzymes EC5.4.99.5, EC5.3.1.6, EC3.5.3.1, EC2.3.1.1, and EC2.7.2.11 ( Figure S5 , Table S4 ). Besides, PAS_chr4_0645, PAS_chr4_0974, PAS_chr1-4_0489, and PAS_chr2-2_0288 encoding enzymes EC2.6.1.11, EC2.6.1.1, EC4.4.1.1, and EC3.5.3.1 were only up-regulated in PI2 other than PI1. In general, most of the genes involved in the amino acid...”
- “...were detected, and those were all down-regulated ( Figure S5 ). The expression level of PAS_chr4_0645 (EC2.6.1.11) and PAS_chr1-4_0160 (EC3.6.1.31/3.5.4.19/1.1.1.23) participating in the arginine and histidine biosynthesis process decreased further in PI2 in comparison to PI1 ( Table S5 ). In conclusion, our transcriptomic study indicated...”
NJ7G_0260 aspartate aminotransferase family protein from Natrinema sp. J7-2
41% identity, 93% coverage
LIMLP_08770 aspartate aminotransferase family protein from Leptospira interrogans serovar Manilae
39% identity, 93% coverage
- Leptospira interrogans biofilm transcriptome highlights adaption to starvation and general stress while maintaining virulence
Davignon, NPJ biofilms and microbiomes 2024 - “...(LIMLP_08670, FC 1.6) and LIMLP_06420 (FC 1.8), four showed downregulation, argJ (LIMLP_16730, FC 1.9), argD (LIMLP_08770, FC 1.5), argB (LIMLP_18295, FC 1.6), and glnA (LIMLP_11985, FC 2.0). A similar profile was observed in histidine metabolism, with one gene being upregulated ( hisI /LIMLP_02360, FC 2.4), and...”
SERP0545 ornithine aminotransferase from Staphylococcus epidermidis RP62A
37% identity, 94% coverage
Ava_0214 Aminotransferase class-III from Anabaena variabilis ATCC 29413
38% identity, 86% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...PCC 73102 Npun02002114 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena variabilis ATCC 29413 Ava_0127 Ava_0214, Ava_3730, Ava_2839 Ava_2942, Ava_1554, Ava_3534, Ava_2258 N.d. = not detected. Table 7 Database entries of genes from 24 cyanobacterial genomes encoding putative L-arginine amidinotransferases (C1), L-ornithine transaminases (C2), and 1...”
- “...PCC 73102 Npun02001803 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena variabilis ATCC 29413 Ava_2273 Ava_0214, Ava_3730, Ava_2839 Ava_2942, Ava_1554, Ava_3534, Ava_2258 N.d. = not detected. Table 8 Database entries of genes from 24 cyanobacterial genomes encoding putative L-arginine deiminases (D1), L-ornithine transcarbamoylases (D2), carbamate kinases...”
Deima_2454 aspartate aminotransferase family protein from Deinococcus maricopensis DSM 21211
38% identity, 90% coverage
- Evolution of lysine biosynthesis in the phylum deinococcus-thermus
Nishida, International journal of evolutionary biology 2012 - “...Deinococcus deserti Deide_09240 Deide_16910 Deide_17960 Deide_10430 Deide_10350 Deide_13430* Deide_13460* Deide_13470* Deide_13980 Deinococcus maricopensis Deima_0046 Deima_1545 Deima_2454 Deima_2593 Deima_1346* Deima_1349* Deima_1350* Deima_1353* Deima_1355* Deima_1358* Deinococcus proteolyticus Deipr_0213 Truepera radiovictrix Trad_2841 Trad_1401* Trad_1404* Trad_1399* Trad_1395* Trad_1392* Trad_1390* Trad_1389* Trad_1388* Enzyme 1, -aminoadipate aminotransferase. Enzyme 2, Homoisocitrate dehydrogenase. Enzyme...”
PF1685 acetylornithine aminotransferase from Pyrococcus furiosus DSM 3638
38% identity, 96% coverage
SXYL_01965 ornithine--oxo-acid transaminase from Staphylococcus xylosus
36% identity, 94% coverage
alr2398 4-aminobutyrate aminotransferase from Nostoc sp. PCC 7120
37% identity, 86% coverage
- Bioinformatic evaluation of L-arginine catabolic pathways in 24 cyanobacteria and transcriptional analysis of genes encoding enzymes of L-arginine catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Schriek, BMC genomics 2007 - “...PCC 7421 n.d. Glr0547, Glr3849, Gll2223 Glr2755, Glr3848, Gll1504, Gll2805 Nostoc sp. PCC 7120 Alr2310 Alr2398, Alr1080, All0396 Alr0540, Alr3771, All3556, All5022 Nostoc punctiforme PCC 73102 Npun02002114 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena variabilis ATCC 29413 Ava_0127 Ava_0214, Ava_3730, Ava_2839 Ava_2942, Ava_1554, Ava_3534, Ava_2258...”
- “...PCC 7421 Glr1758 Glr0547, Glr3849, Gll2223 Glr2755, Glr3848, Gll1504, Gll2805 Nostoc sp. PCC 7120 Alr4495 Alr2398, Alr1080, All0396 Alr0540, Alr3771, All3556, All5022 Nostoc punctiforme PCC 73102 Npun02001803 Npun02005728, Npun02001164, Npun02001509 Npun02003702, Npun02006572, Npun02002895, Npun02002692 Anabaena variabilis ATCC 29413 Ava_2273 Ava_0214, Ava_3730, Ava_2839 Ava_2942, Ava_1554, Ava_3534, Ava_2258...”
PAB2440 acetylornithine aminotransferase from Pyrococcus abyssi GE5
38% identity, 95% coverage
OAT_BACSU / P38021 Ornithine aminotransferase; OAT; Ornithine--oxo-acid aminotransferase; EC 2.6.1.13 from Bacillus subtilis (strain 168) (see 2 papers)
BSU40340 ornithine--oxo-acid transaminase from Bacillus subtilis subsp. subtilis str. 168
37% identity, 93% coverage
- function: Catalyzes the interconversion of ornithine to glutamate semialdehyde.
catalytic activity: a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid (RHEA:13877)
cofactor: pyridoxal 5'-phosphate
disruption phenotype: Cells lacking this gene do not grow in minimal medium containing either arginine or ornithine as sole nitrogen source. - rocF affects the production of tetramethylpyrazine in fermented soybeans with Bacillus subtilis BJ3-2
Liu, BMC biotechnology 2022 - “...Down BSU37760 2661.551 110.143 4.59 4.99E-07 6.28E-05 Down BSU01630 639.373 31.384 4.34 2.85E-06 3.06E-04 Down BSU40340 9319.344 465.809 4.32 2.35E-06 2.60E-04 Down BSU18310 796.701 42.378 4.23 3.91E-06 3.97E-04 Down BSU38560 1224.887 65.094 4.23 4.36E-06 4.23E-04 Down BSU40320 6670.251 373.473 4.16 6.14E-06 5.47E-04 Down BSU18300 795.359 44.677...”
- A Survey of Pyridoxal 5'-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis
Richts, Frontiers in molecular biosciences 2019 - “...Threonine utilization Unknown BioF (45%)/ PLP AAT class II family Nicolas et al., 2012 RocD BSU40340 No 2.6.1.13 3RUY ( B. anthracis , 76%) Ornithine transaminase Arginine, ornithine and citrulline utilization SigL, Spo0A (), CodY (), AhrC (+), RocR (+) PLP AAT class III family, OAT...”
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...P67910, P37759, P0AC88, P27830), Class III Aminotransferase family (P36839, P94427, P53555, P71084, P30949, P33189, O34662, P38021, P22256, P50457, P12995, P23893, P48247, P18335, P77581, O30508, Q9I606, Q9I6 M4, Q9I693, Q9I700, Q9I6J2, Q9I6R7, Q9HV04, Q9HTP1, Q9HWU0, Q9HT50, P48247, Q9I168, P12677, Q82 MM1, P21267, P40732, Q8ZPV2, Q8ZLX7), Thiamine Diphosphate...”
UH47_09195 ornithine--oxo-acid transaminase from Staphylococcus pseudintermedius
38% identity, 93% coverage
BT3758 N-succinylornithine aminotransferase (EC 2.6.1.81) from Bacteroides thetaiotaomicron VPI-5482
BT3758 acetylornithine aminotransferase from Bacteroides thetaiotaomicron VPI-5482
40% identity, 97% coverage
- mutant phenotype: Important for fitness in most defined media, except when arginine is provided. Similar to acetylornithine aminotransferases (such as argD from B. subtilis), but Bacteroidetes use succinylated intermediates for arginine synthesis (see PMC7311316).
- GapMind: Automated Annotation of Amino Acid Biosynthesis
Price, mSystems 2020 - “...mutants of this gene were at low abundance in the starting samples. Third, GapMind identified BT3758 as a potential aminotransferase for converting N -acylglutamate semialdehyde to N -acylornithine but with moderate confidence, because it was less than 40% identical to any characterized enzyme. BT3758 also is...”
AM326 acetylornithine aminotransferase from Anaplasma marginale str. St. Maries
39% identity, 96% coverage
PH1716 acetylornithine aminotransferase from Pyrococcus horikoshii OT3
37% identity, 96% coverage
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...aminotransferase Kynurenine 2-OG, OAA, 2-oxobutyrate, 2-oxo-4-methylthiobutyrate Okada et al. (2012) PH0377 G8 (Histidinol-phosphate aminotransferase) II PH1716 (Long hypothetical acetylornithine aminotransferase) PH1501 (Long hypothetical aminotransferase) PH1423 Ornithine -aminotransferase l -Orn, l -Lys, d -Orn, d -Lys, (5-aminovalerate, 6-aminohexanoate, GABA) 2-OG, (Pyr) Kawakami et al. (2022) PH0782 Alanine/serine...”
- The primordial metabolism: an ancestral interconnection between leucine, arginine, and lysine biosynthesis
Fondi, BMC evolutionary biology 2007 - “...as well as in the AAA variant of lysine biosynthesis, and that ORF PH1720, PH1718, PH1716, and PH1715 (Figure 3b ) might be involved in the biosynthesis of both lysine (through the AAA one) and arginine. Even though it cannot be ruled a priori out the...”
- Functional and evolutionary relationship between arginine biosynthesis and prokaryotic lysine biosynthesis through alpha-aminoadipate
Miyazaki, Journal of bacteriology 2001 - “...unlike in Pyrococcus horikoshii, which possesses argDE homologs (PH1716 and PH1715) in a tandem manner at a position distal to the putative lysine biosynthetic...”
- “...in amino acid sequence to a putative Pyrococcus homolog, PH1716. It should be noted that LysJ is more closely related to ArgD homologs from archaea than to...”
DRA0029, DR_A0029 4-aminobutyrate aminotransferase from Deinococcus radiodurans R1
39% identity, 80% coverage
Igni_0944 N2-acetyl-L-lysine aminotransferase from Ignicoccus hospitalis KIN4/I
37% identity, 97% coverage
- Multi-omics analysis provides insight to the Ignicoccus hospitalis-Nanoarchaeum equitans association
Rawle, Biochimica et biophysica acta. General subjects 2017 - “...culture STRING interaction map ( Figure 2 ). I. hospitalis Culture Gene Identifier Annotation Type Igni_0944 N2-acetyl-L-lysine aminotransferase t N2-Acetyl-L-Lysine m Igni_0945 Hypothetical protein t Igni_0445 Succinate dehydrogenase iron-sulfur subunit m Igni_0085 Succinyl-CoA synthetase (ADP-forming) alpha subunit t Igni_0086 Succinyl-CoA synthetase (ADP-forming) beta subunit t Succinate...”
ARG8 / P18544 acetylornithine transaminase (EC 2.6.1.11) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 9 papers)
ARGD_YEAST / P18544 Acetylornithine aminotransferase, mitochondrial; ACOAT; EC 2.6.1.11 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
YOL140W Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine from Saccharomyces cerevisiae
37% identity, 88% coverage
- function: catalyzes the conversion of N-acetylglutamate-gamma- semialdehyde (NAGSA) to N-acetylornithine in arginine biosynthesis.
catalytic activity: N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L- glutamate 5-semialdehyde + L-glutamate (RHEA:18049)
cofactor: pyridoxal 5'-phosphate - The Formation of Neochromosomes during Experimental Evolution in the Yeast Saccharomyces cerevisiae
Thierry, Genes 2021 - “...with the Y. lipolytica gene ( YALI0F16291g ) replacing KRS1 of S. cerevisiae and with YOL140w marking the left arm of chromosome XV (BYAT581-120/3 not shown). The 770 kb band hybridizes with probes YDL057w marking the left end of chromosome IV (BYAT581-60 is weak but visible)...”
- “...replaced by the band migrating at ca. 585 kb showing brighter hybridizations with probes YDR030c, YOL140W , and YALI0F16291g but no hybridization with the probe YDL057w. The presence of a 4.1 kb amplified fragment using primers AT354 and AT361 for PCR amplification on DNA from strains...”
- Intracellular trafficking of the pyridoxal cofactor. Implications for health and metabolic disease
Whittaker, Archives of biochemistry and biophysics 2016 - “...aminotransferase AGAT 2.6.1.44 Agx1 YFL030W Alanine transaminase ALT 2.6.1.2 Alt1 YLR089C Acetylornithine aminotransferase 2.6.1.11 Arg8 YOL140W Kynurenine aminotransferase KAT 2.6.1.7 Bna3 YJL060W L-serine (L-threonine) deaminase (catabolic) 4.3.1.19 Cha1 YCL064C Glycine decarboxylase complex GCC 2.1.2.10 Gcv2 YMR189W 5-Aminolevulinate synthase ALAS 2.3.1.37 Hem1 YDR232W Threonine deaminase TD 4.3.1.19...”
- Identification of conserved gene clusters in multiple genomes based on synteny and homology
Sarkar, BMC bioinformatics 2011 - “...to correctly distinguish the single orthog gene clusters from the YGR019w UGA1 group. For the YOL140w ARG8 Acetylornithine aminotransferase group, both SONS and SYNS algorithms provide similar conserved gene clusters. However, SONS erroneously mixes some genes of this group with YGR019w UGA1 cluster and YNR058w BIO3...”
- Metabolic pathway relationships revealed by an integrative analysis of the transcriptional and metabolic temperature stress-response dynamics in yeast
Walther, Omics : a journal of integrative biology 2010 - “...2.6.1.41, 1779000_at YJR148W, 2.6.1.42, 1777344_at YOL140W, 2.6.1.11, 1772375_at YPR145W, 6.3.5.4, 1772615_at YJL045W, 1.3.5.1, 1774888_at 3.38E-04 3.49E-04...”
- Full dynamic range proteome analysis of S. cerevisiae by targeted proteomics
Picotti, Cell 2009 - “...YJL026W 40968192 8 5 YKR048C, YER006W, YML086C, YKR001C, YER003C 20484096 9 5 YFL014W, YDR129C, YPL235W, YOL140W, YMR170C YEL031W, YHR107C, YPR118W, YJR051W 10242048 10 10 YDL021W, YML100W, YKL150W, YEL031W, YGL202W, YDL017W, YGR080W, YPL049C, YGL248W, YEL011W YMR170C, YCL017C 5121024 11 10 YHR107C, YGL100W, YBR208C, YPR118W, YJL172W, YBR283C, YCR088W,...”
- Modulation of yeast genome expression in response to defective RNA polymerase III-dependent transcription
Conesa, Molecular and cellular biology 2005 - “...YMR062C YMR094W YMR095C YMR096W YMR097C YMR108W YMR189W YNL104C YOL058W YOL140W PYC2 ILV6 HIS4 ARO3 HOM2 PIC2 ARG5,6 TMT1 ARG4 BAT1 HIS5 ARG3 CPA2 SRY1 ECM40...”
- Novel archaeal alanine:glyoxylate aminotransferase from Thermococcus litoralis
Sakuraba, Journal of bacteriology 2004 - “...NP_418478a P04694 P06986 P07172 P16246 P17736 P17731 P18335 P18544 P04181 P07991 P28269 P22256 P17649 P14010 P19938 P00510 P21549 P23721 P19689 P10658 1708172Aa...”
Cabys_3334 ornithine--oxo-acid transaminase from Caldithrix abyssi DSM 13497
40% identity, 93% coverage
BL1061 acetylornithine aminotransferase from Bifidobacterium longum NCC2705
39% identity, 83% coverage
SCO1577 acetylornithine aminotransferase from Streptomyces coelicolor A3(2)
42% identity, 94% coverage
- Transcriptomic analysis of a classical model of carbon catabolite regulation in Streptomyces coelicolor
Romero-Rodríguez, BMC microbiology 2016 - “...oxidoreductase SCO0556 Hypothetical protein SCO1570 argH, SCL24.06c Argininosuccinate lyase [EC:4.3.2.1] SCO1576 argR, SCL24.12c Arginine repressor SCO1577 argD, SCL24.13c Acetonitrile aminotransferase [EC:2.6.1.11] SCO1578 argB, SCL24.14c Acetylglutamate kinase [EC:2.7.2.8] SCO1579 argJ, SCL24.15c Putative glutamate N-acetyltransferase [EC:2.3.1.1 2.3.1.35] SCO1580 argC N-Acetyl-gamma-glutamyl-phosphate reductase [EC:1.2.1.38] SCO1815 fabG, SCI28.09c Probable 3-oxacyl-(acyl-carrier-protein) reductase...”
bll2855 ornithine aminotransferase from Bradyrhizobium japonicum USDA 110
37% identity, 92% coverage
BB341_RS23960, SCLAV_0798 acetylornithine transaminase from Streptomyces clavuligerus
41% identity, 93% coverage
Deide_09040 putative 4-aminobutyrate aminotransferase, aminotransferase class-III from Deinococcus deserti VCD115
37% identity, 82% coverage
- Proteomics-based refinement of Deinococcus deserti genome annotation reveals an unwonted use of non-canonical translation initiation codons
Baudet, Molecular & cellular proteomics : MCP 2010 - “.../ z range of an N-terminally TMPP-labeled peptide, TIAIKPR, corresponding to the N terminus of Deide_09040, which exemplifies the most intense of these reporter ions in most spectra with signals from m / z 573.16, 646.13, and 674.03 ions. We used mainly these three reporter ions...”
- “...during fragmentation of unmodified peptides. The MS/MS spectrum of the TMPP-modified peptide TIAIKPR assigned to Deide_09040 is shown in B . The reporter ion TMPP-CHCO + and the corresponding a1 - and b1 -specific ions are labeled in bold red . Correction of Protein N Termini...”
Echvi_3848 N-succinylornithine aminotransferase (EC 2.6.1.81) from Echinicola vietnamensis KMM 6221, DSM 17526
42% identity, 97% coverage
- mutant phenotype: Important for fitness in most defined media, except when arginine or citrulline is provided. Similar to acetylornithine aminotransferases (such as argD from B. subtilis), but Bacteroidetes use succinylated intermediates for arginine synthesis (see PMC7311316).
BBIF_1100 acetylornithine transaminase from Bifidobacterium bifidum S17
41% identity, 84% coverage
- Proteomic Profiling of Bifidobacterium bifidum S17 Cultivated Under In Vitro Conditions
Wei, Frontiers in microbiology 2016 - “...262 29.1 BBIF_0863 ilvE2 Branched-chain amino acid aminotransferase 375 41.5 BBIF_0870 bbif_0870 Aminotransferase 522 58.1 BBIF_1100 argD Acetylornithine aminotransferase 429 45.4 BBIF_1175 bbif_1175 Aspartate aminotransferase 394 43.5 BBIF_1428 serC Phosphoserine aminotransferase 380 40.6 BBIF_1519 bbif_1519 N-succinyldiaminopimelate aminotransferase 392 41.5 BBIF_1610 aspC2 Aspartate aminotransferase 409 44.5 a...”
5eqcA / S8EY38 Structure of the ornithine aminotransferase from toxoplasma gondii crystallized in presence of oxidized glutathione reveals partial occupancy of plp at the protein active site
35% identity, 87% coverage
- Ligands: alpha-d-glucopyranose; beta-d-glucopyranose; pyridoxal-5'-phosphate (5eqcA)
S8EY38 ornithine aminotransferase (EC 2.6.1.13) from Toxoplasma gondii (see paper)
TGME49_069110, TGME49_269110, XP_002365604 ornithine aminotransferase, mitochondrial precursor, putative from Toxoplasma gondii ME49
35% identity, 84% coverage
- From TgO/GABA-AT, GABA, and T-263 Mutant to Conception of Toxoplasma
Lykins, iScience 2024 - “...mutation could have functional significance ( Figure10 ). Additionally, MacRae etal. 42 predicted Tg OAT (TGME49_269110) was the most likely candidate for the GABA-AT that converts GABA to succinate semialdehyde, and TGME49_057480 as the NADP-dependent succinate semialdehyde dehydrogenase that converts succinate semialdehyde to succinate, which then...”
- “...were utilized. At Dartmouth, KU80 knockout background, 191 the Type II Tg O/GABA-AT gene locus (TGME49_269110) was deleted in the Pru ku80 hxgprt (this paper). T. gondii Pru ( https://www.nature.com/articles/ncomms10147#article-info ) EGS strain T.gondii is ATCC PRA-396 . RH strain T.gondii ( https://www.nature.com/articles/ncomms10147#article-info ). Me49 strain...”
- CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in Toxoplasma gondii
Lykins, Frontiers in cellular and infection microbiology 2018 - “...IDs: Tg PGM: TGME49_297060, Tg NDK: TGME49_295350, Tg RPE: TGME49_247670, Tg RPI: TGME49_239310, Tg OAT: TGME49_269110. Results Structural genomic pipeline for Toxoplasma The NIAID Structural Genomics Centers selected 265 proteins for the Toxoplasma Structural Genomic Pipeline in three phases (Figure 1 ). Phase I and II...”
- Unique substrate specificity of ornithine aminotransferase from Toxoplasma gondii.
Astegno, The Biochemical journal 2017 (PubMed)- GeneRIF: Ornithine aminotransferase from Toxoplasma gondii dispalys unique substrate specificity.
- Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle
Possenti, BMC genomics 2013 - “...28 26 4.03E-12 TGME49_059180 cystathionine beta-synthase, putative 4.2.1.22 amino acid metabolism n.a. n.a. n.a. 8.37E-13 TGME49_069110 ornithine aminotransferase, putative 2.6.1.13 amino acid metabolism 394 123 247 2.54E-12 TGME49_078910 O-acetylserine (thiol) lyase, putative 2.5.1.47 amino acid metabolism 20 3 5 2.39E-10 TGME49_112930 cystathionine beta-lyase, putative 4.4.1.1 amino...”
- “...for the synthesis of 6 non essential amino acids of T. gondii , i.e., proline (TGME49_069110), alanine (TGME49_115260, TGME-49_003500), threonine (TGME49_016640), cysteine (TG-ME49_059180, TGME49_078910, TGME49_112930), lysine (TGME49_005420) and tyrosine (TGME49_08-7510, TGME49_012740). The two forms of alanine dehydrogenase (EC 1.4.1.1), which catalyses the unidirectional conversion of pyruvate...”
Dgeo_0989 aminotransferase class-III from Deinococcus geothermalis DSM 11300
39% identity, 83% coverage
TTC0553 No description from Thermus thermophilus HB27
39% identity, 89% coverage
Rru_A3466 Ornithine aminotransferase from Rhodospirillum rubrum ATCC 11170
36% identity, 89% coverage
T643_RS11485 4-aminobutyrate--2-oxoglutarate transaminase from Klebsiella pneumoniae MRSN 1319
39% identity, 86% coverage
- Multidrug resistant pathogens respond differently to the presence of co-pathogen, commensal, probiotic and host cells
Chan, Scientific reports 2018 - “...total RNA. For K . pneumoniae , 2 downregulated ( acnB , T643_RS19850; gabT _2, T643_RS11485) and 3 upregulated (aminotransferase, T643_RS18930; cimH , T643_RS19470; PTS system fructose IIA component, T643_RS18905) genes were tested. For A . baumannii , 3 downregulated ( papD , T634_RS02055; mmsA _1,...”
SPISAL_02400 aspartate aminotransferase family protein from Spiribacter salinus M19-40
35% identity, 87% coverage
- Compatible Solute Synthesis and Import by the Moderate Halophile Spiribacter salinus: Physiology and Genomics
León, Frontiers in microbiology 2018 - “...search of the S. salinus M19-40 genome. In this way, we found three EctB-related proteins: SPISAL_02400, SPISAL_06590, and SPISAL_07805 ( Len et al., 2013 ; Lopez-Perez et al., 2013 ). To distinguish the potential EctB enzyme from the two other transaminases, we used the orthology IDs...”
- “...ornithine-carbamoyl-transferase (ArgF; SPISAL_06595). Bona fide EctB enzymes belong to the orthology group K00836-ectB but the SPISAL_02400 protein was not listed under this identification number in KEGG database. Instead, it was affiliated with two orthology groups: K00823-puuE (comprising 4-aminobutyrate aminotransferases) and K00821-argD. Since EctB is a L-2,4-diaminobutyrate-2-oxoglutarate...”
CAC0368 4 animobutyrate aminotransferase from Clostridium acetobutylicum ATCC 824
36% identity, 83% coverage
J7QVE2 ornithine aminotransferase from Methylocystis sp. (strain SC2)
35% identity, 86% coverage
SCO1223 ornithine aminotransferase from Streptomyces coelicolor A3(2)
37% identity, 91% coverage
- Impact of Phosphate Availability on Membrane Lipid Content of the Model Strains, Streptomyces lividans and Streptomyces coelicolor
Lejeune, Frontiers in microbiology 2021 - “...of PhoR/PhoP and we confirmed that it was indeed the case ( Figure 2A ). sco1223 encodes a ornithine aminotransferase, related to RocD of Bacillis subtilis , involved in the conversion of pyrroline-5-carboxylate, originating from glutamate or proline degradation, into ornithine. sco1222 encodes a protein of...”
- “...Level of expression of sco0921 and sco0920 (black and white histograms, respectively) and sco1222 and sco1223 (dark and light gray histograms, respectively) in the three strains. S. lividans TK24 was taken as reference equal to 1. Means values are shown as histograms with error bars representing...”
- Coelimycin Synthesis Activatory Proteins Are Key Regulators of Specialized Metabolism and Precursor Flux in Streptomyces coelicolor A3(2)
Bednarz, Frontiers in microbiology 2021 - “...in comparison to M145 strain ( Figure 3A ). Interestingly, we have detected SCO1222 and SCO1223, proteins probably belonging to one transcriptional unit having 66 and 82% identity, respectively, to the corresponding proteins of salinomycin BGC from Streptomyces albus ( Medema et al., 2015 ). These...”
- “...( Alam et al., 2011 ). As for now, the pathway that involves SCO1222 and SCO1223 has still not been resolved. Transcriptional Profiling of Coelimycin Biosynthetic Gene Cluster In order to gain an insight into the mechanism of coelimycin synthesis regulation, we employed dense time-point luciferase...”
- Gamma-Glutamylpolyamine Synthetase GlnA3 Is Involved in the First Step of Polyamine Degradation Pathway in Streptomyces coelicolor M145
Krysenko, Frontiers in microbiology 2017 - “...Perez-Redondo et al., 2012 ). Further genetic analysis of SCO5655 and other potential aminotransferase homologs (SCO1223, SCO1284, and SCO6769) would be required to determine whether a PatA-dependent pathway is functional. The regulation and importance of glutamylation and aminotransferase pathways may dependent on polyamine concentration or another...”
- Uncovering genes with divergent mRNA-protein dynamics in Streptomyces coelicolor
Jayapal, PloS one 2008 - “...correlated with PC-1 of both proteins and mRNA. Genes involved in arginine-glutamate conversion like RocD (SCO1223) and ArgH (SCO1570) displayed a prominent correlation with PC-1 only at the protein level. In contrast, a glycine cleavage system (SCO1378/5472, GcvP/T; glycine degradation), a serine hydroxymethyltransferase (SCO5470, GlyA; serine-glycine...”
PA14_03450 4-aminobutyrate aminotransferase from Pseudomonas aeruginosa UCBPP-PA14
37% identity, 84% coverage
davT / Q9I6M4 5-aminovalerate aminotransferase (EC 2.6.1.48) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
DAVT_PSEAE / Q9I6M4 5-aminovalerate aminotransferase DavT; 5-aminovalerate transaminase; Delta-aminovalerate aminotransferase; EC 2.6.1.48 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
Q9I6M4 5-aminovalerate transaminase (EC 2.6.1.48) from Pseudomonas aeruginosa (see paper)
PA0266 4-aminobutyrate aminotransferase from Pseudomonas aeruginosa PAO1
37% identity, 84% coverage
- function: Catalyzes the conversion of 5-aminovalerate to 5- oxopentanoate.
catalytic activity: 5-aminopentanoate + 2-oxoglutarate = 5-oxopentanoate + L- glutamate (RHEA:10212)
cofactor: pyridoxal 5'-phosphate - Differential transcription profiling of the phage LUZ19 infection process in different growth media
Brandão, RNA biology 2021 (secret) - The development of a new parameter for tracking post-transcriptional regulation allows the detailed map of the Pseudomonas aeruginosa Crc regulon
Corona, Scientific reports 2018 - “...Hypothetical protein 1,39 0,96 1,33 Other siaB PA0171 Hypothetical protein 1,5 1,44 2,6 Catabolism gabT PA0266 Delta-aminovalerate aminotransferase 1,69 0,21 1,1 Catabolism spuA PA0297 Probable glutamine amidotransferase 0,39 0,6 2,11 Catabolism spuB PA0298 Glutamylpolyamine synthetase 0,51 0,53 2,01 Catabolism spuC PA0299 Polyamine:pyruvate transaminase 0,57 0,31 1,46...”
- A systems-level approach for investigating Pseudomonas aeruginosa biofilm formation
Xu, PloS one 2013 - “...PA0228 ( pcaF ) acetate metabolism Rxn#5 : 4-Aminobutanoate + 2-Oxoglutarate L-Glutamate + Succinic semialdehyde PA0266 ( gabT ) glutamate production Rxn#6 : 2 S-Adenosyl-L-methionine + Uroporphyrinogen III 2 S-Adenosyl-L-homocysteine + H+ + Dihydrosirohydrochlorin PA0510 ( nirE ) coenzyme B12 synthesis Rxn#7 : Ferrocytochrome c +...”
- Regulatory and metabolic networks for the adaptation of Pseudomonas aeruginosa biofilms to urinary tract-like conditions
Tielen, PloS one 2013 - “...which do not possess a Fur-binding box, as for example the cyo -operon (PA1317PA1321), gabT (PA0266), or PA4633 encoding a potential chemotaxis transducer. These genes were found up regulated in the experiment performed by Ochsner and coworker but not in our experiments [66] . Moreover, they...”
- Structure and activity of the Pseudomonas aeruginosa hotdog-fold thioesterases PA5202 and PA2801
Gonzalez, The Biochemical journal 2012 - “...deletion on the expression of three groups of P. aeruginosa genes involved in ketone-body metabolism (PA0266, PA0447, PA1999, PA2003, PA2011, and PA5015), pyocyanin biosynthesis (PA4209, PA4210, PA4211, and PA4214), and pyocyanin-induced shock response (PA2274, PA4205, and PA4206) ( Table 3 ). After three hours of growth,...”
- “...encoded or gene name Relative expression 2 PA5202 (LB) wild type (LB--KBA) PA5202 (LB--KBA) I PA0266 4-aminobutyrate aminotransferase 1.6 0.1 0.60 0.01 0.80 0.01 PA0447 Glutaryl-CoA dehydrogenase 0.60 0.01 0.30 0.01 0.20 0.02 PA1999 3-ketoacid-CoA transferase 1.0 0.1 1.0 0.1 0.70 0.01 PA2003 3-Hydroxybutyrate dehydrogenase 1.4...”
- Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1
Yao, Journal of bacteriology 2011 - “...transaminase Transcriptional regulator; LysR family PA0265 PA0266 gabD gabT 448 721 6,923 9,244 4,434 6,136 Succinate semialdehyde dehydrogenase...”
- The novel Pseudomonas aeruginosa two-component regulator BfmR controls bacteriophage-mediated lysis and DNA release during biofilm development through PhdA
Petrova, Molecular microbiology 2011 - “...PA5210 - - - probable secretion pathway ATPase PA4938 purA - - - adenylosuccinate synthetase PA0266 gabT - - - 4-aminobutyrate aminotransferase Protein detected on a silver stained 2D-PAGE gel; - Protein not detected on a silver stained 2D-PAGE gel. Table 2 COMSTAT analysis a of...”
- The novel two-component regulatory system BfiSR regulates biofilm development by controlling the small RNA rsmZ through CafA
Petrova, Journal of bacteriology 2010 - “...factor IF-2 Exoribonuclease RNase R Metabolism PA0266 PA1546 gabT hemN gltA sdhB 4-Aminobutyrate aminotransferase Oxygen-independent coproporphyrinogen III...”
- “...PA1905/PA4215 PA1904 PA4498 PA1914 PA2300 PA4217 PA1800 PA2385 PA0266 PA3326 Gene 5280 PETROVA AND SAUER J. BACTERIOL. TABLE 4. Comparison of the bfiS-dependent...”
- More
- Protein Interaction Networks of Catalytically Active and Catalytically Inactive PqsE in Pseudomonas aeruginosa
Taylor, mBio 2022 - “...cysD cysD 1.092340172 Q02H29 murC murC 1.224317298 P27726 gap gapA 1.354759487 Q51363 nadB nadB 1.354759487 Q9I6M4 davT PA14_03450 1.411195433 B7UYA0 ackA ackA 1.486004021 P30718 groL groL 1.708396442 Q02H54 groS groS 1.76611194 P20581 pqsE pqsE 2.005782353 P54291 rhlI rhlI 9.965784285 TABLE2 Log 2 fold change comparison between...”
BC0355 4-aminobutyrate aminotransferase from Bacillus cereus ATCC 14579
35% identity, 79% coverage
- Temporal dynamics of stress response in Halomonas elongata to NaCl shock: physiological, metabolomic, and transcriptomic insights
Yu, Microbial cell factories 2024 - “...of POD, CAT, and GST enzyme were measured using commercial kits (BC0025, BC0095, BC0205, and BC0355, Solarbio), following the manufacturers recommendations. RNA extraction and transcriptome sequencing Harvested cells from 5min before shock (1% NaCl condition), 5 and 10min after shock for both 8% and 13% NaCl...”
- Physiological and biochemical characteristics and microbial responses of Medicago sativa (Fabales: Fabaceae) varieties with different resistance to atrazine stress
Li, Frontiers in microbiology 2024 - “...665 Enzyme activity determination and physiological indicator detection According to the kit manuals (BC0090, BC0170, BC0355, BC0200, BC0020; Beijing Solaibao Technology Co., Ltd., China), the activities of peroxidase (POD), superoxide dismutase (SOD), Glutathione S -transferase (GST), catalase (CAT), and malondialdehyde (MDA) were determined. Subsequently, oxidative damage...”
- Bacillus cereus ATCC 14579 RpoN (Sigma 54) Is a Pleiotropic Regulator of Growth, Carbohydrate Metabolism, Motility, Biofilm Formation and Toxin Production
Hayrapetyan, PloS one 2015 - “...by Sigma 54 in B . thuringiensis [ 20 , 21 , 47 ]. GabT (BC0355) and Lysine 2.3-aminomutase (BC2251) were predicted to be in the regulon of Sigma 54 in B . cereus (this study, Table C in S1 File ). Both of these genes...”
STM2792 4-aminobutyrate aminotransferase from Salmonella typhimurium LT2
STM14_3368 4-aminobutyrate--2-oxoglutarate transaminase from Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
36% identity, 84% coverage
- Inorganic Polyphosphate Is Essential for Salmonella Typhimurium Virulence and Survival in Dictyostelium discoideum
Varas, Frontiers in cellular and infection microbiology 2018 - “...STM14_2999 STM2441 cysA Sulfate/thiosulfate transporter subunit STM14_3031 STM2472 maeB Malic enzyme STM14_3058 Hypothetical protein STM14_3368 STM2792 gabT 4-aminobutyrate aminotransferase STM14_3463 STM2866 sprB Transcriptional regulator STM14_3529 STM2926 pcm Protein-L-isoaspartate O-methyltransferase STM14_3594 STM2980 ygdE Putative RNA 2'-O-ribose methyltransferase STM14_3709 STM3069 pgk Phosphoglycerate kinase STM14_3969 STM3286 infB Translation initiation...”
- Inorganic Polyphosphate Is Essential for Salmonella Typhimurium Virulence and Survival in Dictyostelium discoideum
Varas, Frontiers in cellular and infection microbiology 2018 - “...thiolase STM14_2999 STM2441 cysA Sulfate/thiosulfate transporter subunit STM14_3031 STM2472 maeB Malic enzyme STM14_3058 Hypothetical protein STM14_3368 STM2792 gabT 4-aminobutyrate aminotransferase STM14_3463 STM2866 sprB Transcriptional regulator STM14_3529 STM2926 pcm Protein-L-isoaspartate O-methyltransferase STM14_3594 STM2980 ygdE Putative RNA 2'-O-ribose methyltransferase STM14_3709 STM3069 pgk Phosphoglycerate kinase STM14_3969 STM3286 infB Translation...”
PH1501 aminotransferase from Pyrococcus horikoshii OT3
34% identity, 86% coverage
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...2-oxo-4-methylthiobutyrate Okada et al. (2012) PH0377 G8 (Histidinol-phosphate aminotransferase) II PH1716 (Long hypothetical acetylornithine aminotransferase) PH1501 (Long hypothetical aminotransferase) PH1423 Ornithine -aminotransferase l -Orn, l -Lys, d -Orn, d -Lys, (5-aminovalerate, 6-aminohexanoate, GABA) 2-OG, (Pyr) Kawakami et al. (2022) PH0782 Alanine/serine racemase Ala, Ser *** Kawakami...”
- TK1211 Encodes an Amino Acid Racemase towards Leucine and Methionine in the Hyperthermophilic Archaeon Thermococcus kodakarensis
Zheng, Journal of bacteriology 2021 (secret) - A Novel PLP-Dependent Alanine/Serine Racemase From the Hyperthermophilic Archaeon Pyrococcus horikoshii OT-3
Kawakami, Frontiers in microbiology 2018 - “...(BAR) from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3. Three genes, PH0782 , PH1423 , and PH1501 , encoding homologs exhibiting about 45% sequence identity with BAR were present in the P. horikoshii genome. In this study, we detected pyridoxal 5-phosphate (PLP)-dependent amino acid racemase activity in...”
- “...PLP-dependent enzyme. Three other genes within the P. horikoshii genome, PH0782 , PH1423 , and PH1501 , encode proteins that are also annotated as GABA-ATs and exhibit high similarity to BAR. This suggests these homologs also function as amino acid racemases. We previously reported that BAR...”
HD73_0366 4-aminobutyrate--2-oxoglutarate transaminase from Bacillus thuringiensis serovar kurstaki str. HD73
35% identity, 83% coverage
- Identification of metabolism pathways directly regulated by sigma(54) factor in Bacillus thuringiensis
Peng, Frontiers in microbiology 2015 - “...Table 3 Genes controlled by 54 in B. thuringiensis HD73 . Genes ID Annotation Fold-change HD73_0366 4-aminobutyrate aminotransferase 9.165 HD73_0367 Sensory box sigma-54 dependent DNA-binding response regulator 2.860 HD73_0368 Succinic semialdehyde dehydrogenase 2.410 HD73_0369 Quaternary ammonium compound-resistance protein 2.377 HD73_0560 Biotin carboxyl carrier protein - HD73_0561...”
- “...work. We previously characterized the expression of the GABA pathway gene cluster in which gabT (HD73_0366) is controlled by 54 and activated by GabR (Zhu et al., 2010 ), which specifically bound to a repeat region 58 bp upstream of the start codon (Peng et al.,...”
t2687 4-aminobutyrate aminotransferase from Salmonella enterica subsp. enterica serovar Typhi Ty2
35% identity, 84% coverage
A0A3B6MXE9 ornithine aminotransferase (EC 2.6.1.13) from Triticum aestivum (see paper)
37% identity, 79% coverage
ESA_01203 hypothetical protein from Enterobacter sakazakii ATCC BAA-894
36% identity, 81% coverage
FUT48_13600 4-aminobutyrate--2-oxoglutarate transaminase from Pseudomonas sp. JG-B
36% identity, 84% coverage
GabT / b2662 4-aminobutyrate aminotransferase GabT (EC 2.6.1.19; EC 2.6.1.48; EC 2.6.1.11) from Escherichia coli K-12 substr. MG1655 (see 29 papers)
gabT / P22256 4-aminobutyrate aminotransferase GabT (EC 2.6.1.19; EC 2.6.1.48; EC 2.6.1.11) from Escherichia coli (strain K12) (see 28 papers)
GABT_ECOLI / P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 6 papers)
NP_417148 4-aminobutyrate aminotransferase GabT from Escherichia coli str. K-12 substr. MG1655
b2662 4-aminobutyrate aminotransferase from Escherichia coli str. K-12 substr. MG1655
36% identity, 85% coverage
- function: Pyridoxal phosphate-dependent enzyme that catalyzes transamination between primary amines and alpha-keto acids. Catalyzes the transfer of the amino group from gamma-aminobutyrate (GABA) to alpha-ketoglutarate (KG) to yield succinic semialdehyde (SSA) and glutamate (PubMed:15723541, PubMed:30498244). Thereby functions in a GABA degradation pathway that allows some E.coli strains to utilize GABA as a nitrogen source for growth (PubMed:12446648). Also catalyzes the conversion of 5-aminovalerate to glutarate semialdehyde, as part of a L-lysine degradation pathway that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate (PubMed:30498244).
catalytic activity: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (RHEA:23352)
catalytic activity: 5-aminopentanoate + 2-oxoglutarate = 5-oxopentanoate + L- glutamate (RHEA:10212)
cofactor: pyridoxal 5'-phosphate
subunit: Homotetramer.
disruption phenotype: Cells lacking this gene are not able to utilize GABA as a nitrogen source, in contrast to wild-type, but grow normally with arginine, ornithine, putrescine and agmatine (PubMed:12446648). Cells show only 68% of the wild-type GABA aminotransferase activity (PubMed:20639325). - Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: isolation and characterization of a phosphinothricin-specific transaminase from Escherichia coli.
Schulz, Applied and environmental microbiology 1990 - GeneRIF: N-terminus verified by Edman degradation on complete protein
- Evolutionary origin and functional diversification of aminotransferases
Koper, The Journal of biological chemistry 2022 - “...enzyme ( Fig.5 B ). Based on the crystal structure of E.coli GABA AT (gabT, P22256) ( 140 ) ( Fig.5 ), an additional S112 residue interacts with the phosphate group, and V241 and Q242 interact with the pyridine ring of PLP ( Fig.5 C )....”
- Knowns and Unknowns of Vitamin B6 Metabolism in Escherichia coli
Tramonti, EcoSal Plus 2021 (secret) - Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...P37759, P0AC88, P27830), Class III Aminotransferase family (P36839, P94427, P53555, P71084, P30949, P33189, O34662, P38021, P22256, P50457, P12995, P23893, P48247, P18335, P77581, O30508, Q9I606, Q9I6 M4, Q9I693, Q9I700, Q9I6J2, Q9I6R7, Q9HV04, Q9HTP1, Q9HWU0, Q9HT50, P48247, Q9I168, P12677, Q82 MM1, P21267, P40732, Q8ZPV2, Q8ZLX7), Thiamine Diphosphate Decarboxylase...”
- Novel archaeal alanine:glyoxylate aminotransferase from Thermococcus litoralis
Sakuraba, Journal of bacteriology 2004 - “...P16246 P17736 P17731 P18335 P18544 P04181 P07991 P28269 P22256 P17649 P14010 P19938 P00510 P21549 P23721 P19689 P10658 1708172Aa P12995 P22805 Accession number...”
- Predicting functional family of novel enzymes irrespective of sequence similarity: a statistical learning approach.
Han, Nucleic acids research 2004 - “...2.7 Intestinalguanylate cyclase (P70106) EC 4.6 2.60E12 E1->F1;E2->F1 Glutamate-1-semialdehyde aminotransferase (Q06774) EC 5.4 4-aminobutyrate aminotransferase (P22256) EC 2.6 5.70E32 E1->F1;E2->F2 + Exodeoxyribonuclease (P37454) EC 3.1 DNA- (apurinic or apyrimidinic site) lyase (P43138) EC 4.2 1.60E96 E1->F1;E2->F2 + E1-> F1 or E2 -> F2 indicates that enzyme...”
- Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions
Orth, BMC systems biology 2012 - “...identified yrbG (b3196) none identified kdsC (b3198) none identified argD (b3359) astC (b1748) 1.00E-146 gabT (b2662) 3.00E-64 puuE (b1302) 3.00E-54 patA (b3073) 4.00E-52 hemL (b0154) 3.00E-32 cysG (b3368) none identified ilvE (b3770) pabC (b1096) 8.00E-8 dapF (b3809) none identified argC (b3958) none identified argB (b3959) none...”
- Homotaurine metabolized to 3-sulfopropanoate in Cupriavidus necator H16: enzymes and genes in a patchwork pathway
Mayer, Journal of bacteriology 2009 - “...2-oxoglutarate aminotransferase; GABA transaminase) (EC 2.6.1.19; b2662), whose structure has been published (33); and the dehydrogenase GabD...”
- Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones
Lee, Applied and environmental microbiology 2009 - “...b1745 b1746 b1747 b1748 b2000 b2097 b2210 b2465 b2661 b2662 b2663 b2724 b2873 b3012 b3049 b3175 b3230 b3311 b3426 b3447 b3453 b1488 b3540 b3541 b3542 b3588...”
- In silico atomic tracing by substrate-product relationships in Escherichia coli intermediary metabolism
Arita, Genome research 2003 - “...(EC 2.6.1.19), coded in the genes goaG (b1302) and gabT (b2662). However, this enzyme uses L-Glu as an amino acceptor, and L-Ala was shown not to be an acceptor...”
1sf2A / P22256 Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
36% identity, 85% coverage
- Ligands: sulfate ion; pyridoxal-5'-phosphate (1sf2A)
A1S_3281 4-aminobutyrate aminotransferase PLP-dependent from Acinetobacter baumannii ATCC 17978
39% identity, 87% coverage
K4A9B8 Ornithine aminotransferase from Setaria italica
37% identity, 78% coverage
hpnO / B3QHB5 aminobacteriohopanetriol synthase from Rhodopseudomonas palustris (strain TIE-1) (see paper)
37% identity, 77% coverage
PHATRDRAFT_27726 ornithine aminotransferase from Phaeodactylum tricornutum CCAP 1055/1
35% identity, 81% coverage
A0A3B6LSQ4 ornithine aminotransferase (EC 2.6.1.13) from Triticum aestivum (see paper)
37% identity, 79% coverage
8r2pD / P38507,P42588 Yzwideal x16 a scaffold for cryo-em of small proteins of interest crystallizing in space group 19 (p 21 21 21)
35% identity, 74% coverage
- Ligand: pyridoxal-5'-phosphate (8r2pD)
PatA / b3073 putrescine aminotransferase (EC 2.6.1.29) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
patA / P42588 putrescine aminotransferase (EC 2.6.1.82; EC 2.6.1.29) from Escherichia coli (strain K12) (see 15 papers)
PAT_ECOLI / P42588 Putrescine aminotransferase; PAT; PATase; Cadaverine transaminase; Diamine transaminase; Putrescine transaminase; Putrescine--2-oxoglutaric acid transaminase; Putrescine:2-OG aminotransferase; EC 2.6.1.82; EC 2.6.1.29 from Escherichia coli (strain K12) (see 8 papers)
P42588 putrescine-2-oxoglutarate transaminase (EC 2.6.1.82) from Escherichia coli (see 4 papers)
b3073 putrescine:2-oxoglutaric acid aminotransferase, PLP-dependent from Escherichia coli str. K-12 substr. MG1655
NP_417544 putrescine aminotransferase from Escherichia coli str. K-12 substr. MG1655
ETEC_3343 putrescine aminotransferase from Escherichia coli ETEC H10407
35% identity, 83% coverage
- function: Catalyzes the aminotransferase reaction from putrescine to 2- oxoglutarate, leading to glutamate and 4-aminobutanal, which spontaneously cyclizes to form 1-pyrroline (PubMed:12617754, PubMed:3510672). This is the first step in one of two pathways for putrescine degradation, where putrescine is converted into 4- aminobutanoate (gamma-aminobutyrate or GABA) via 4-aminobutanal, which allows E.coli to grow on putrescine as the sole nitrogen source (PubMed:22636776, PubMed:3510672). Also functions as a cadaverine transaminase in a a L-lysine degradation pathway to succinate that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate (PubMed:12617754, PubMed:30498244). Is also able to transaminate spermidine, in lower extent, but not ornithine. Alpha-ketobutyrate and pyruvate can also act as amino acceptors, although much less efficiently (PubMed:12617754).
catalytic activity: an alkane-alpha,omega-diamine + 2-oxoglutarate = an omega- aminoaldehyde + L-glutamate (RHEA:18217)
catalytic activity: putrescine + 2-oxoglutarate = 1-pyrroline + L-glutamate + H2O (RHEA:12268)
catalytic activity: cadaverine + 2-oxoglutarate = 5-aminopentanal + L-glutamate (RHEA:61624)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer.
disruption phenotype: Cells lacking this gene show a high decrease in putrescine aminotransferase activity, but are still able to grow with putrescine as the sole nitrogen source. However, a mutant lacking both patA and either puuA, puuB or puuC cannot grow with putrescine as the sole nitrogen source. - Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity
Krysenko, Medical sciences (Basel, Switzerland) 2022 - “...permease PuuP (b1296)/PlaP (b2014) PA5510 SCO5057 Lysine/ornithine decarboxylase-like enzyme - - SCO5651 Pyruvate-polyamine aminotransferase PatA (b3073) SpuC (PA0299) SCO5655 Lrp/AsnC family transcriptional regulator - - SCO5656 -aminobutyraldehyde or -glutamyl--amino-butyraldehyde dehydrogenase PatD (b1444)/PuuC (b1300) BetB (PA5373)/PAO219 SCO5657 Polyamine-binding lipoprotein PotF (b0854) SpuD (PA0300) SCO5658 -aminobutyraldehyde dehydrogenase or...”
- Genome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding
Bonocora, PLoS genetics 2015 - “...- b2887 ygfT 36 4.529 OS33 3217457 10 G TGGC GCAATCCC TGC A 3217461 + b3073 patA 36 8.711 C OS34 3416975 29 C TGGC ACTACTT TTGC T 3416975 + b3268 yhdW 70 12.521 P OS35 3556144 17 C TGGC ACGACGG TTGC A 3556148 - b3421...”
- Genome-scale analysis of escherichia coli FNR reveals complex features of transcription factor binding
Myers, PLoS genetics 2013 - “...None [18] 1,030,741 yccA b0970 Putative Transport Protein 1 71.5 o None None 3,217,259 ygjG b3073 Putrescine Aminotransferase 1 96.5 None None 1,014,801 rmf b0953 Ribosome Modulation Factor 1 98.5 + o None None 4,231,491 lysC b4024 Aspartate Kinase III 2 115.5 + o None None...”
- Pathway and enzyme redundancy in putrescine catabolism in Escherichia coli
Schneider, Journal of bacteriology 2012 - “...are indicated in the bottom half, except for patA (b3073). The solid arrows indicate the genes and the direction of transcription, drawn to scale. There are...”
- Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions
Orth, BMC systems biology 2012 - “...(b3198) none identified argD (b3359) astC (b1748) 1.00E-146 gabT (b2662) 3.00E-64 puuE (b1302) 3.00E-54 patA (b3073) 4.00E-52 hemL (b0154) 3.00E-32 cysG (b3368) none identified ilvE (b3770) pabC (b1096) 8.00E-8 dapF (b3809) none identified argC (b3958) none identified argB (b3959) none identified hemE (b3997) none identified ubiC...”
- Promoter and regulon analysis of nitrogen assimilation factor, sigma54, reveal alternative strategy for E. coli MG1655 flagellar biosynthesis
Zhao, Nucleic acids research 2010 - “...for HycE of hydrogenlyase 3 Energy metabolism, carbon: Anaerobic respiration 2.4 1.3 46 29 acacTGGCACAATTATTGCTTGtagc b3073 ygjG putative acetylornithine aminotransferase, PLP-dependent Amino acid biosynthesis: Arginine 7.1 1.2 35 18 ggagTGGCGCAATCCCTGCAATactt a b no. indicates Blattner number. b It is possible that one gene has several different...”
- Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity
Weber, Journal of bacteriology 2005 - “...ycaC yfcF ygjG yhfW yjcS yjgB yncG b0453 b0897 b2301 b3073 b3380 b4083 b4269 b1454 ysgA b3830 Regulation (11) bolA b0435 chaB csiR b1217 b2664 gadW b3515 gadX...”
- Sulfur and nitrogen limitation in Escherichia coli K-12: specific homeostatic responses
Gyaneshwar, Journal of bacteriology 2005 - “...oval in panel B are ompF (b0929), yeaG (b1783), and ygiG (b3073). The gene in the green oval for glutathione is cbl, which is also included in the genes in the...”
- More
- Two mechanisms for putrescine-dependent transcriptional expression of the putrescine aminotransferase gene, ygjG, in Escherichia coli.
Kim, Archives of microbiology 2014 (PubMed)- GeneRIF: rpoS is required for putrescine-dependent ygjG-lacZ expression, but the effect of putrescine on this expression is not caused by simple modulation of RpoS synthesis
- Crystallization and preliminary X-ray crystallographic analysis of YgjG from Escherichia coli.
Yeo, Acta crystallographica. Section F, Structural biology and crystallization communications 2012 - GeneRIF: crystal belonged to the primitive orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 121.1, b = 129.5, c = 131.3 A, and is estimated to contain four molecules of YgjG per asymmetric unit
- Molecular cloning and characterization of Escherichia coli K12 ygjG gene.
Samsonova, BMC microbiology 2003 - GeneRIF: Expression of E. coli K12 ygjG coding region revealed sigma54-depended ORF which encodes a 459-amino acid protein with putrescine:2-OG aminotransferase activity.
- Halomonas elongata: a microbial source of highly stable enzymes for applied biotechnology
Benítez-Mateos, Applied microbiology and biotechnology 2023 - “...composition of a halotolerant aminotransferase enzyme (Uniprot: E1V913) and its homolgous from E. coli (Uniprot: P42588). Asp and Glu residues are highlighted in red, while Phe, Leu, and Ile are depicted in white Apart from their negatively charged exposed residues, the composition of bulky hydrophobic residues...”
- Knowns and Unknowns of Vitamin B6 Metabolism in Escherichia coli
Tramonti, EcoSal Plus 2021 (secret) - Crystallization and preliminary X-ray crystallographic analysis of YgjG from Escherichia coli
Yeo, Acta crystallographica. Section F, Structural biology and crystallization communications 2012 - “...of YgjG The gene encoding YgjG (UniProt accession No. P42588) was amplified by polymerase chain reaction (PCR) with Pfu DNA polymerase from chromosomal DNA of...”
- Molecular cloning and characterization of Escherichia coli K12 ygjG gene
Samsonova, BMC microbiology 2003 - “...gene of 1290 bp (denoted downstream as ORF1) which codes 429-amino acid polypeptide (SWISS-PROT Database, P42588) is situated 126 nucleotides downstream of the mRNA start. Analysis of the nucleotide sequence revealed the other ORF (denoted as ORF2) encoding 459-amino acid protein with UUG initiation codon (Fig....”
- Integrated Metabolic Modeling, Culturing, and Transcriptomics Explain Enhanced Virulence of Vibrio cholerae during Coinfection with Enterotoxigenic Escherichia coli
Abdel-Haleem, mSystems 2020 - “...Co-culture2993, however, are able to degrade putrescine into glutamate by putrescine transaminase ( patA : ETEC_3343) or into glutamate and succinate through the gamma-glutamyl putrescine synthetase ( puuA : ETEC_1401)/oxidoreductase ( puuB : ETEC_1405) pathway, both being absent in the V. cholerae genome. Similarly, V. cholerae...”
B1A0U3 ornithine aminotransferase (EC 2.6.1.13) from Pisum sativum (see 2 papers)
36% identity, 79% coverage
ECs3955 putrescine--2-oxoglutarate aminotransferase from Escherichia coli O157:H7 str. Sakai
35% identity, 81% coverage
- Whole-Transcriptome Analysis of Verocytotoxigenic Escherichia coli O157:H7 (Sakai) Suggests Plant-Species-Specific Metabolic Responses on Exposure to Spinach and Lettuce Extracts
Crozier, Frontiers in microbiology 2016 - “...; 38121-fold induced), heavy-metal resistance (e.g., cusBX ; 5681-fold induced), putrescine metabolism (e.g., ygjG , ECs3955; 5265-fold induced), multidrug eux (e.g., sugE ; 33-fold induced) and osmotic stress ( proVW ; 25-fold). In addition, a cluster of genes (ECs1653-1655; 1428-fold) of unknown function was strongly induced...”
- Global transcriptional response of Escherichia coli O157:H7 to growth transitions in glucose minimal medium
Bergholz, BMC microbiology 2007 - “...ygiS -2.33 1 ECs3278 gltX -2.47 1 ECs3948 rpsU -3.14 1 ECs3286 cysK -3.68 1 ECs3955 ygjG 5.55 2 ECs3293 cysA -2.71 1 ECs3963 ygjL 4.06 2 ECs3302 yfeX -2.24 1 ECs3979 yqjC 2.12 2 ECs3322 - 2.10 2 ECs3980 yqjD 2.28 2 ECs3323 - 2.15...”
A0A3B6KM96 ornithine aminotransferase (EC 2.6.1.13) from Triticum aestivum (see paper)
37% identity, 79% coverage
Sant_1573 putrescine aminotransferase from Sodalis praecaptivus
37% identity, 82% coverage
WP_000069444 4-aminobutyrate--2-oxoglutarate transaminase from Shigella dysenteriae
35% identity, 85% coverage
- The scale-free nature of protein sequence space
Buchholz, PloS one 2018 - “...dehydrogenase subunit Gammaproteo-bacteria WP_044256366 266 oTA putrescine aminotransferase Enterobacter cloacae WP_042715413 381 aspartate aminotransferase Shigella WP_000069444 378 SDR GDP-mannose 4,6-dehydratase Helicobacter pylori WP_058338748 312 Dimensions of protein sequence networks As a further network property, the fractal network dimension D f was evaluated by counting the number...”
ACICU_03477 4-aminobutyrate--2-oxoglutarate transaminase from Acinetobacter baumannii ACICU
YP_001848133 4-aminobutyrate aminotransferase from Acinetobacter baumannii ACICU
39% identity, 82% coverage
- Transcriptome Remodeling of Acinetobacter baumannii during Infection and Treatment
Wright, mBio 2017 - “...insertion in a gene encoding another membrane protein (ACICU_03345). ABUH66276 has a nonsynonymous mutation in ACICU_03477, a predicted 4-aminobuytrate transaminase, and the expression of adjacent ACICU_03476 to ACICU_03479, part of the 4-aminobutyrate catabolic pathway, is significantly increased. IS-associated deletion of ACICU_01775 to ACICU_01779 was present in...”
- Norspermidine is not a self-produced trigger for biofilm disassembly
Hobley, Cell 2014 - “...B.subtilis NCIB3610 [6051-HGW] GabT (YP_007532319); BmegGabT, B.megaterium QM B1551 GabT (YP_003564524); AbauGabT, A.baumannii ACICU GabT (YP_001848133); BsubRocD, B.subtilis NCIB3610 RocD (YP_007536035); BsubArgD, B.subtilis NCIB3610 ArgD (YP_007533057); AbauDABAAT, A.baumannii ACICU DABA AT (YP_001847319); BmegDABAAT, B.megaterium QM B1551 DABA AT (YP_003564515); VchoDABAAT, V.cholerae O1 str. EM-1676A DABA AT...”
EX895_002715 hypothetical protein from Sporisorium graminicola
34% identity, 54% coverage
- Molecular Mechanisms Involved in the Multicellular Growth of Ustilaginomycetes
Martínez-Soto, Microorganisms 2020 - “...reilianum a,b Sporisorium graminicola a Testicularia cyperi a,b,d Acetylornithine aminotransferase OAT UMAG_05671 UHOR_07330 UBRO_07330 sr15996 EX895_002715 BCV70DRAFT_201257 Ornithine decarboxylas ODC UMAG_01048 UHOR_01580 UBRO_01580 sr12348 EX895_006099 BCV70DRAFT_212462 Polyamine oxidase PAO UMAG_05850 UHOR_08347 UBRO_08347 sr16473 EX895_008244 BCV70DRAFT_173429 Chimeric spermidine synthase/saccharopine reductase SPE UMAG_05818 UHOR_08297 UBRO_08297 sr16440 EX895_003211 BCV70DRAFT_199161...”
PY17X_0109000 ornithine aminotransferase, putative from Plasmodium yoelii
34% identity, 88% coverage
XP_006280404 ornithine aminotransferase, mitochondrial from Capsella rubella
35% identity, 78% coverage
blr3010 blr3010 from Bradyrhizobium japonicum USDA 110
37% identity, 77% coverage
- Pleiotropic Effects of PhaR Regulator in Bradyrhizobium diazoefficiens Microaerobic Metabolism
Quelas, International journal of molecular sciences 2024 - “...production from glutamate. Moreover, ornithine concentration may also be increased due to downregulation of rocD (blr3010), which encodes an ornithine aminotransferase (EC 2.6.1.13) that removes ornithine from this pathway towards proline synthesis ( Table S3 ). Increased flux to ornithine might favor citruline synthesis, the next...”
- “...the top ten candidates, either repressed (blr4358, blr4582, blr4687, blr6331) or activated by PhaR (blr2131, blr3010, bll3872), was next verified by DNA electrophoretic mobility shift assays (EMSAs) ( Figure 6 ). The promoter region of the genuine PhaR target phaP1 (bll5155) was also included in the...”
GoaG / b1302 4-aminobutyrate aminotransferase PuuE (EC 2.6.1.19; EC 2.6.1.48) from Escherichia coli K-12 substr. MG1655 (see 12 papers)
puuE / P50457 4-aminobutyrate aminotransferase PuuE (EC 2.6.1.19; EC 2.6.1.48) from Escherichia coli (strain K12) (see 11 papers)
PUUE_ECOLI / P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see 2 papers)
puuE / BAD88710.1 gamma-aminobutyrate aminotransferase from Escherichia coli K-12 (see paper)
NP_415818 4-aminobutyrate aminotransferase PuuE from Escherichia coli str. K-12 substr. MG1655
b1302 GABA aminotransferase, PLP-dependent from Escherichia coli str. K-12 substr. MG1655
36% identity, 86% coverage
- function: Catalyzes the transfer of the amino group from gamma- aminobutyrate (GABA) to alpha-ketoglutarate (KG) to yield succinic semialdehyde (SSA). PuuE is important for utilization of putrescine as the sole nitrogen or carbon source.
catalytic activity: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (RHEA:23352)
cofactor: pyridoxal 5'-phosphate
disruption phenotype: Cells show only 35% of the wild-type activity. - Knowns and Unknowns of Vitamin B6 Metabolism in Escherichia coli
Tramonti, EcoSal Plus 2021 (secret) - Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...P0AC88, P27830), Class III Aminotransferase family (P36839, P94427, P53555, P71084, P30949, P33189, O34662, P38021, P22256, P50457, P12995, P23893, P48247, P18335, P77581, O30508, Q9I606, Q9I6 M4, Q9I693, Q9I700, Q9I6J2, Q9I6R7, Q9HV04, Q9HTP1, Q9HWU0, Q9HT50, P48247, Q9I168, P12677, Q82 MM1, P21267, P40732, Q8ZPV2, Q8ZLX7), Thiamine Diphosphate Decarboxylase family...”
- Genetic organization of the mecA region in methicillin-susceptible and methicillin-resistant strains of Staphylococcus sciuri
Wu, Journal of bacteriology 1998 - “...mecA region (98) CTORF179 of K1 mecA region (99) P50457 Y09223 Y09223 S. sciuri subsp. rodentius K8 mecA region ORF142 NTORF101 Unknown-function protein of B....”
- Spot 42 RNA regulates putrescine catabolism in Escherichia coli by controlling the expression of puuE at the post-transcription level.
Sun, Journal of microbiology (Seoul, Korea) 2021 (PubMed)- GeneRIF: Spot 42 RNA regulates putrescine catabolism in Escherichia coli by controlling the expression of puuE at the post-transcription level.
- A systems approach discovers the role and characteristics of seven LysR type transcription factors in Escherichia coli
Rodionova, Scientific reports 2022 - “...Gamma-glutamyl-gamma-aminobutyrate hydrolase PuuD 6.21E05 337 1.9 b1299 puuR HTH-type transcriptional regulator PuuR 9.24E05 113 1.3 b1302 puuE 4-aminobutyrate aminotransferase PuuE 3.16E04 161 1.6 b1495 nuoK NADH-quinone oxidoreductase subunit K 4.59E07 73 1.9 b1496 yddA Inner membrane ABC transporter ATP-binding protein YddA 1.25E06 63 2.3 b1526 yneJ...”
- Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity
Krysenko, Medical sciences (Basel, Switzerland) 2022 - “...SCO5670 -glutamyl-polyamine oxidoreductase PuuB (b1301) PauB3 (PA2776) SCO5671 -aminobutyrate aminotransferase gabT-like or puuE-like GabT (b2662)/PuuE (b1302) GabT (PA266) SCO5676 Succinate-semialdehyde dehydrogenase gabD-like GabD (b2661) GabD (PA0265) SCO5679 Amino acids/polyamine permease PuuP (b1296) PA5510/PAO322 SCO5977 Hydrolase - - SCO6960 Amidohydrolase - - SCO6961 -glutamyl-polyamine synthetase PuuA (b1297)...”
- 18th Congress of the European Hematology Association, Stockholm, Sweden, June 13–16, 2013
, Haematologica 2013 - Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions
Orth, BMC systems biology 2012 - “...none identified kdsC (b3198) none identified argD (b3359) astC (b1748) 1.00E-146 gabT (b2662) 3.00E-64 puuE (b1302) 3.00E-54 patA (b3073) 4.00E-52 hemL (b0154) 3.00E-32 cysG (b3368) none identified ilvE (b3770) pabC (b1096) 8.00E-8 dapF (b3809) none identified argC (b3958) none identified argB (b3959) none identified hemE (b3997)...”
- Extensive genomic diversity in pathogenic Escherichia coli and Shigella Strains revealed by comparative genomic hybridization microarray
Fukiya, Journal of bacteriology 2004 - “...The two regions were JW1289 to JW1295 (b1296 to b1302) and JW2466 to JW2476 (b2481 to b2491). The former region included genes encoding a putative amino acid...”
- “...(JW1289, b1296, ycjJ) and 4-aminobutyrate aminotransferase (JW1295, b1302, goaG). The latter consisted of the hyf operon coding for hydrogenase-4 for anaerobic...”
- In silico atomic tracing by substrate-product relationships in Escherichia coli intermediary metabolism
Arita, Genome research 2003 - “...transaminase (EC 2.6.1.19), coded in the genes goaG (b1302) and gabT (b2662). However, this enzyme uses L-Glu as an amino acceptor, and L-Ala was...”
B8ANL7 Ornithine aminotransferase from Oryza sativa subsp. indica
36% identity, 80% coverage
OAT_PLAF7 / Q6LFH8 Ornithine aminotransferase; PfOAT; Ornithine--oxo-acid aminotransferase; EC 2.6.1.13 from Plasmodium falciparum (isolate 3D7) (see 2 papers)
Q6LFH8 ornithine aminotransferase (EC 2.6.1.13) from Plasmodium falciparum (see 2 papers)
PF3D7_0608800, XP_966078 ornithine aminotransferase from Plasmodium falciparum 3D7
34% identity, 88% coverage
- function: The enzyme has a very narrow substrate specificity and can only catalyze the transamination of alpha-ketoglutarate with ornithine or N-acetylornithine and, to a lesser extent, of glutamate-5- semialdehyde with glutamate and alanine.
catalytic activity: a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid (RHEA:13877)
catalytic activity: L-ornithine + 2-oxoglutarate = L-glutamate 5-semialdehyde + L- glutamate (RHEA:25160)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Photoaffinity probe-based antimalarial target identification of artemisinin in the intraerythrocytic developmental cycle of Plasmodium falciparum
Gao, iMeta 2024 - “...validation of the targeting of artesunate (ATS) to Plasmodium falciparum ornithine aminotransferase ( PfOAT ) (PF3D7_0608800). (A) ATS increases the level of H 2 O 2 of P. falciparum . (B) ATS decreases the level of GSH of P. falciparum . (C) Schematic diagram of protein...”
- “...proteins (Figure 6C ). A notable example is P. falciparum ornithine aminotransferase ( PfOAT , PF3D7_0608800), which directly affects polyamine synthesis by regulating ornithine levels and is involved in the regulation of glucose metabolism and cell proliferation [ 40 , 41 ]. The active site Cys...”
- Identification of antimalarial targets of chloroquine by a combined deconvolution strategy of ABPP and MS-CETSA
Gao, Military Medical Research 2022 - “...The genes of L-lactate dehydrogenase ( Pf LDH, PF3D7_1324900 ), ornithine aminotransferase ( Pf OAT, PF3D7_0608800 ), pyruvate kinase ( Pf PyrK, PF3D7_0626800 ), phosphoglycerate kinase ( Pf PGK, PF3D7_0922500 ) and triosephosphate isomerase ( Pf TPI, PF3D7_1439900 ) of P. falciparum 3D7 were synthesized commercially...”
- “...involved in glycolysis and energy metabolism including Pf LDH ( PF3D7_1324900 ), Pf OAT ( PF3D7_0608800 ), Pf PyrK ( PF3D7_0626800 ), Pf PGK ( PF3D7_0922500 ) and Pf TPI ( PF3D7_1439900 ). To start with, we retrieved the structures of these 5 proteins from PDB...”
- A nuclear redox sensor modulates gene activation and var switching in Plasmodium falciparum
Heinberg, Proceedings of the National Academy of Sciences of the United States of America 2022 - “...found three proteins: thioredoxin peroxidase I ( Pf TPx-1, PF3D7_1438900), ornithine aminotransferase ( Pf OAT, PF3D7_0608800), and elongation factor 2 (PF3D7_1451100) ( Table 1 , boldface). Given the reasons below, we chose to focus on Pf TPx-1. Table 1. Nuclear proteins identified by affinity chromatography in...”
- “...* * PF3D7_1438900 Thioredoxin peroxidase 1 50-fold 42-fold Metabolism PF3D7_0513300 Purine nucleoside phosphorylase * * PF3D7_0608800 Ornithine aminotransferase * * PF3D7_0922500 Phosphoglycerate kinase * * PF3D7_1324900 L-lactate dehydrogenase * * PF3D7_1444800 Fructose-bisphosphate aldolase * * PF3D7_1462800 Glyceraldehyde-3-phosphate dehydrogenase * * Translation PF3D7_0309600 60S acidic ribosomal protein...”
- Peroxide Antimalarial Drugs Target Redox Homeostasis in Plasmodium falciparum Infected Red Blood Cells
Siddiqui, ACS infectious diseases 2022 - “...probe compounds, such as translationally controlled tumor protein (PF3D7_0511000), 72 actin 1 (PF3D7_1246200), ornithine aminotransferase (PF3D7_0608800), and the glycolytic enzymes, lactate dehydrogenase (PF3D7_1324900), enolase (PF3D7_1015900), and glyceraldehyde-3-phosphate dehydrogenase (PF3D7_1462800), 21 23 were confirmed to be alkylation targets for AA2 and OZ727 but were not alkylated by...”
- Identification of sulfenylation patterns in trophozoite stage Plasmodium falciparum using a non-dimedone based probe
Schipper, Molecular and biochemical parasitology 2021 - “...Purine nucleoside phosphorylase 26.9 71.4% / 69.8% / 82.0% C71 C141 C208 + + 3 PF3D7_0608800 Ornithine aminotransferase 46.0 65.9% / 54.1% / 40.6% C63 + + 4 PF3D7_0621200 Pyridoxine biosynthesis protein PDX1 33.0 63.1% / 62.5% / 63.1% C16 C177 + + 5 PF3D7_0810800 Dihydropteroate...”
- “...plasmoredoxin (Plrx) [ 14 ]. PlasmoDB Accession n Protein name Trx Grx Plrx SOH 1 PF3D7_0608800 Ornithine aminotransferase + + + + 2 PF3D7_0626800 Pyruvate kinase + + + + 3 PF3D7_0708400 Heat shock protein 90 + + + + 4 PF3D7_0802200 1-cys peroxiredoxin + +...”
- Integration of population and functional genomics to understand mechanisms of artemisinin resistance in Plasmodium falciparum
Oberstaller, International journal for parasitology. Drugs and drug resistance 2021 - “...membrane protein 1, PfEMP1 Exported protein, host-cell remodeling PF3D7_1229800 3 Myosin J, putative Vesicular trafficking PF3D7_0608800 3 Ornithine aminotransferase Stress response PF3D7_1119900 3 Protein transport protein SEC16, putative Vesicular trafficking PF3D7_0935900 3 Ring-exported protein 1 Exported protein, host-cell remodeling PF3D7_1145600 3 TMEM65 domain-containing protein, putative Stress...”
- Estimation of parasite age and synchrony status in Plasmodium falciparum infections
Ciuffreda, Scientific reports 2020 - “...analysed to select four genes matching either early ( PF3D7_0301800, PF3D7_1002000 ) or late ( PF3D7_0608800 , PF3D7_1035800 ) gene expression over the first 24h post erythrocyte-invasion (ring stage). To assess their validity as parasite age markers in RT-qPCR, their gene expression relative to the reference...”
- “...highest relative gene expression in the early ring stages, PF3D7_1002000 at early-mid ring stage, while PF3D7_0608800 and PF3D7_1035800 presented highest relative gene expression at the late ring stage of the parasite. Relative gene expression in the Pf2004 line was similar to that of HB3 for three...”
- Insights into the intracellular localization, protein associations and artemisinin resistance properties of Plasmodium falciparum K13
Gnädig, PLoS pathogens 2020 - “...7 (3,4) PF3D7_1037100 Pyruvate kinase 2 PyKII Apicoplast / Isoprenoid metobolismmetabolism 1.19% 4 8 (4,4) PF3D7_0608800 Ornithine aminotransferase OAT Ornithine metabolism 1.18% 3 5 (2,3) PF3D7_1302100 Gamete antigen 27/25 Pfs27/25 Early marker of gametocyte development 1.13% 4 8 (3,5) PF3D7_1327800 Ribose-phosphate pyrophosphokinase, putative -- Pentose phosphate...”
- More
- Global analysis of protein expression and phosphorylation of three stages of Plasmodium falciparum intraerythrocytic development
Pease, Journal of proteome research 2013 - “...26 173 0.20033 0.12982 0.16985 Q8I2J4 profilin 1.94174 10 23 31 0.20973 0.12672 0.16355 Ring Q6LFH8 ornithine aminotransferase 4.9966 10 181 123 0.26665 0.14896 0.08438 Q8IDQ9 phosphoethanolamine N -methyltransferase 2.9399 10 151 148 0.25202 0.14008 0.10789 Q8IJA9 adenosine deaminase 2.7421 10 138 108 0.25039 0.13255 0.11706...”
- Identification of proteins targeted by the thioredoxin superfamily in Plasmodium falciparum
Sturm, PLoS pathogens 2009 - “...9 S -adenosylmethionine synthetase Q7K6A4 PFI1090w 44.8 30 c, +++ 6.28 21 6 Ornithine aminotransferase Q6LFH8 PFF0435w 46.1 18 c, +++ 6.47 16 5 HAP protein/Plasmepsin III Q8IM15 PF14_0078 51.7 48 tm (api), +++ 8.04 16 8 S -adenosyl-L-homocysteine hydrolase Q7K6A6 PFE1050w 53.8 30 c, +++...”
- “...5 S -adenosylmethionine synthetase Q7K6A4 PFI1090w 44.8 30 c, +++ 6.28 9 3 Ornithine aminotransferase Q6LFH8 PFF0435w 46.1 18 c, +++ 6.47 14 6 S -adenosyl-L-homocysteine hydrolase Q7K6A6 PFE1050w 53.8 30 c, +++ 5.64 14 4 Hexokinase Q6LF74 PFF1155w 55.3 12 c, +++ 6.72 11 4...”
Q89LG2 Acetylornithine aminotransferase 2 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)
blr4582 acetylornithine aminotransferase from Bradyrhizobium japonicum USDA 110
39% identity, 95% coverage
- Pleiotropic Effects of PhaR Regulator in Bradyrhizobium diazoefficiens Microaerobic Metabolism
Quelas, International journal of molecular sciences 2024 - “...blr4257blr4264 blr4358 - Q89M35 similar to N-acetyl-gamma-glutamyl-phosphate reductase 8.7 2.6 119 CCGCCAAGATCAAGGCCGCGAA - blr4582 argD2 Q89LG2 acetylornithine aminotransferase 48.9 18.2 286 259 243 170 AGCTGCGCATCC CGCTCGGCGGGC CGCGAAGCGCCC CGCCGCGCAGCT - blr4680 - Q89L68 hypothetical protein 2.8 1.6 235 228 32 CGCCATGCGCTA CGCTACGCGGAC GGCCTCGCGCGA - blr4687 asd Q89L61...”
- Pleiotropic Effects of PhaR Regulator in Bradyrhizobium diazoefficiens Microaerobic Metabolism
Quelas, International journal of molecular sciences 2024 - “...In this regard, the products of blr4358 encoding an N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) and of blr4582 ( argD2 ) encoding one of the two annotated acetylornithine/N-succinyldiaminopimelate aminotransferases (EC 2.6.1.11/2.6.1.17) were also more abundant in the phaR mutant ( Table S3 ). These enzymes catalyze the third...”
- “...interaction of purified PhaR with a selection of the top ten candidates, either repressed (blr4358, blr4582, blr4687, blr6331) or activated by PhaR (blr2131, blr3010, bll3872), was next verified by DNA electrophoretic mobility shift assays (EMSAs) ( Figure 6 ). The promoter region of the genuine PhaR...”
- Bradyrhizobium japonicum senses iron through the status of haem to regulate iron homeostasis and metabolism
Yang, Molecular microbiology 2006 - “...transporter ATP-binding protein bll3192 2.54 0.35 ABC transporter substrate-binding protein bll3193 2.41 0.25 Unknown protein blr4582 14.38 7.22 Acetylornithine aminotransferase blr5037 5.07 0.71 Delta-aminolevulinic acid dehydratase bll5796 3.35 0.47 Fumarase, class I bll5913 4.35 0.90 Cytochrome c blr5962 2.17 0.41 ABC transporter ATP-binding protein blr6742 4.02...”
ABZJ_03667 4-aminobutyrate--2-oxoglutarate transaminase from Acinetobacter baumannii MDR-ZJ06
39% identity, 82% coverage
- Colistin Resistance in Acinetobacter baumannii MDR-ZJ06 Revealed by a Multiomics Approach
Hua, Frontiers in cellular and infection microbiology 2017 - “...0.688359 0.000139 539 384144680 ABZJ_03434 flavoprotein 10 7 15.52 55720.24 9.12 0.685088 0.00015 150 384144913 ABZJ_03667 4-aminobutyrate aminotransferase 55 17 50.23 45976.96 5.81 0.679784 0.000169 1306 384144561 ABZJ_03315 kinase sensor component of a two component signal transduction system 1 1 3.07 62690.76 6.3 0.672652 0.000198 600...”
ECs3523 4-aminobutyrate aminotransferase activity from Escherichia coli O157:H7 str. Sakai
35% identity, 85% coverage
OAT_ORYSJ / Q10G56 Ornithine aminotransferase, mitochondrial; Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase; EC 2.6.1.13 from Oryza sativa subsp. japonica (Rice) (see paper)
36% identity, 79% coverage
XP_007214014 ornithine aminotransferase, mitochondrial from Prunus persica
36% identity, 79% coverage
XP_010494787 ornithine aminotransferase, mitochondrial from Camelina sativa
34% identity, 78% coverage
PP0372, PP_0372 acetylornithine aminotransferase, putative from Pseudomonas putida KT2440
39% identity, 90% coverage
- UEG Week 2024 Poster Presentations
, United European gastroenterology journal 2024 - UEG Week 2023 Poster Presentations
, United European gastroenterology journal 2023 - Transcriptomic Profiling Suggests That Promysalin Alters the Metabolic Flux, Motility, and Iron Regulation in Pseudomonas putida KT2440
Giglio, ACS infectious diseases 2018 - “...fliC to be decreased in promysalin-treated samples compared to the control. Finally, we looked at PP_0372 ( aruC ), involved in ornithine metabolism and up-regulated in our RNA-Seq data, and the expression of PP_0372 was increased (approximately 5-fold). Collectively, the results of the qRT-PCR data showed...”
- Comparative Transcriptome Analysis of Pseudomonas putida KT2440 Revealed Its Response Mechanisms to Elevated Levels of Zinc Stress
Peng, Frontiers in microbiology 2018 - “...- Methionine ABC transporter ATP-binding protein PP_0269 42.33 2.45 61.33 3.95 Glutamate synthase large subunit PP_0372 aruC 95.5 0.12 137.5 6.21 Acetylornithine aminotransferase PP_0699 4.93 0.04 4.8 0.12 LysE family transporter PP_0999 arcC 0.27 0.03 0.23 0.09 Carbamate kinase PP_1110 5.81 1.28 Serine acetyltransferase PP_1400 kgtP...”
NP_001306851 ornithine aminotransferase, mitochondrial from Jatropha curcas
36% identity, 78% coverage
Z3960 4-aminobutyrate aminotransferase activity from Escherichia coli O157:H7 EDL933
35% identity, 85% coverage
Z2486 4-aminobutyrate aminotransferase from Escherichia coli O157:H7 EDL933
36% identity, 86% coverage
argD / CAB82482.1 acetylornithine aminotransferase from Streptomyces clavuligerus (see 3 papers)
40% identity, 94% coverage
XP_011007419 ornithine aminotransferase, mitochondrial from Populus euphratica
35% identity, 78% coverage
A0A674DA32 ornithine aminotransferase (EC 2.6.1.13) from Salmo trutta (see paper)
36% identity, 73% coverage
XP_025460070 ornithine aminotransferase from Aspergillus niger CBS 101883
34% identity, 86% coverage
- Biological Roles of Ornithine Aminotransferase (OAT) in Plant Stress Tolerance: Present Progress and Future Perspectives
Anwar, International journal of molecular sciences 2018 - “...(ABS76054.1), Mycobacterium avium (AAS04411.1), Aspergillus nidulans (Q92413), Saccharomyces cerevisiae (P07991), Neurospora crassa (Q7RX93), Aspergillus lacticoffeatus (XP_025460070), Arabidopsis thaliana (OAO92185), Brassica napus (NP_001303219.1), Glycine max (XP_003531161.1), and Brachypodium distachyon (KQK13994.1). ( B ) Differences in the Glu pathway of Pro synthesis among prokaryotic and eukaryotic organisms. -GK:...”
PBANKA_010740, PBANKA_0107400 ornithine aminotransferase, putative from Plasmodium berghei ANKA
34% identity, 88% coverage
- PhenoMapping: a protocol to map cellular phenotypes to metabolic bottlenecks, identify conditional essentiality, and curate metabolic models
Chiappino-Pepe, STAR protocols 2021 - “...levels Essential gene RNA-seq a Blood phenotype b Liver phenotype c Bottleneck reactions, Alt 1 PBANKA_0107400 Dispensable dispensable UP_R00667_c PBANKA_0516900 Essential no info UP_R00670_c|UP_R00178_c PBANKA_1346500 Dispensable essential UP_R07761_r a Essential gene when transcriptomics data are integrated. b PlasmoGEM phenotypes for blood-stage development of P.berghei . c...”
- “...data are integrated or RNA-gene. Example: in Table 3 , an example of RNA-gene is PBANKA_0107400. 13. Identify each alternative set of bottleneck reaction levels linked to the RNA-gene. Example: PhenoMapping maps the essentiality of the gene to a high flux through cytosolic reaction R00667 (...”
- Stage-Specific Changes in Plasmodium Metabolism Required for Differentiation and Adaptation to Different Host and Vector Environments
Srivastava, PLoS pathogens 2016 - “...a putative GABA/glutamate transaminase which can recycle glutamate, were also obtained ( oat - , PBANKA_010740) and these parasites grew only slightly less quickly than wild type. However, attempts to generate clonal populations of a mutant ( trp - , PBANKA_030670) lacking a putative GABA transporter...”
RLV_7110 4-aminobutyrate--2-oxoglutarate transaminase from Rhizobium leguminosarum bv. viciae
38% identity, 85% coverage
- A host-specific diaminobutyrate aminotransferase contributes to symbiotic performance, homoserine metabolism, and competitiveness in the Rhizobium leguminosarum/Pisum sativum system
Ballesteros-Gutiérrez, Frontiers in microbiology 2023 - “...contrast, the transcript levels of the gene encoding an 4-aminobutyrate aminotransferase (GabT, encoded in locus Rlv_7110), a protein that showed comparable levels in both legume hosts in a previous analysis ( Durn et al., 2021 ), were similar in both hosts (0.85-fold 0.11). Transcripts of the...”
- Proteome Analysis Reveals a Significant Host-Specific Response in Rhizobium leguminosarum bv. viciae Endosymbiotic Cells
Durán, Molecular & cellular proteomics : MCP 2021 - “...RLV_1842 nitrogenase_molybdenum-iron_protein_alpha_chain_NifD 495 264.2 n.d. RLV_1843 putative_nitrogenase_iron_protein_NifH 298 200.0 n.d. RLV_3292 photosystem_reaction_center_subunit_H 202 193.9 22.7 RLV_7110 4-aminobutyrate_aminotransferase 427 192.2 40.0 RLV_1844 diaminobutyrate_aminotransferase 425 182.2 n.d. RLV_4155 histone-like DNA binding protein HU 92 181.5 822.2 RLV_6681 dihydrolipoamide_succinyltransferase 422 177.4 103.2 RLV_6296 heat-shock_protein_IbpA 157 169.4 n.d. RLV_1384 universal_stress_protein...”
- “...proteins also include homologues to two enzymes involved in C/N metabolism, namely gamma-aminobutyrate aminotransferase (GABA-AT, RLV_7110) and succinate semialdehyde dehydrogenase (RLV_7109). These two enzymes have been described in the process of incorporation of GABA from the plant to yield glutamate and succinate ( 46 ). Interestingly,...”
ArtHe_01125 aspartate aminotransferase family protein from Arthrobacter sp. Helios
37% identity, 79% coverage
Q2Z0F8 ornithine aminotransferase (EC 2.6.1.13) from Agaricus bisporus (see paper)
34% identity, 79% coverage
XP_006398303 ornithine aminotransferase, mitochondrial from Eutrema salsugineum
34% identity, 74% coverage
KPHS_46340 putrescine aminotransferase from Klebsiella pneumoniae subsp. pneumoniae HS11286
36% identity, 80% coverage
- Use of a combined antibacterial synergy approach and the ANNOgesic tool to identify novel targets within the gene networks of multidrug-resistant Klebsiella pneumoniae
Lee, mSystems 2024 - “...and forms an integrative network with KPHS_02470 (glucose-6-phosphate), KPHS_09830 (hypothetical protein), KPHS_09840 (hypothetical protein), and KPHS_46340 (putrescine). Notably, a lysine riboswitch is associated with KPHS_02470. ( F ) Decreased levels of KPHS_31520 (phosphoenolpyruvate synthase) and KPHS_31750 (6-phosphofructokinase), along with increased levels of KPHS_51710 (triosephosphate isomerase), KPHS_31180...”
- “...KPHS_23680 (enoyl-CoA hydratase), which is involved in phenylalanine metabolism, is linked to KPHS_23740. Downregulation of KPHS_46340 (putrescine aminotransferases) and KPHS_43350 (acetyl-CoA acetyltransferase) results in a connection with the target gene KPHS_28070, which is a novel noncoding sRNA. KPHS_02470 (glucose-6-phosphate isomerase) is a positively expressed lysine riboswitch-associated...”
OAT_EMENI / Q92413 Ornithine aminotransferase; Ornithine--oxo-acid aminotransferase; EC 2.6.1.13 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
otaA ornithine aminotransferase; EC 2.6.1.13 from Emericella nidulans (see paper)
35% identity, 81% coverage
- catalytic activity: a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid (RHEA:13877)
cofactor: pyridoxal 5'-phosphate - CharProtDB Description: Ornithine transaminase, involved in utilization of arginine as a proline source; expression and activity are arginine-induced; transcription is subject to carbon-catabolite and nitrogen-metabolite repression; regulated by CreA and AreA; Source:AspGD
- Biological Roles of Ornithine Aminotransferase (OAT) in Plant Stress Tolerance: Present Progress and Future Perspectives.
Anwar, International journal of molecular sciences 2018 - “...follows: Bacillus subtilis (NP-391914.1), Streptomyces avermitilis (Q82HT8), Bacillus velezensis (ABS76054.1), Mycobacterium avium (AAS04411.1), Aspergillus nidulans (Q92413), Saccharomyces cerevisiae (P07991), Neurospora crassa (Q7RX93), Aspergillus lacticoffeatus (XP_025460070), Arabidopsis thaliana (OAO92185), Brassica napus (NP_001303219.1), Glycine max (XP_003531161.1), and Brachypodium distachyon (KQK13994.1). ( B ) Differences in the Glu pathway...”
- Estimating the nucleotide diversity in Ceratodon purpureus (Ditrichaceae) from 218 conserved exon-primed, intron-spanning nuclear loci.
McDaniel, Applications in plant sciences 2013 - “...JY262700 AW098585 similar to SW:FKB7_WHEAT Q43207 70 KD PEPTIDYLPROLYL ISOMERASE JY263155 AW098587 similar to SW:OAT_EMENI Q92413 ORNITHINE AMINOTRANSFERASE 547 0.017842 JY262902, JY263051, JY263247, JY262740 AW098597 similar to TR:Q56987 Q56987 HYPOTHETICAL 23.2 KD PROTEIN 254 0 JY263175, JY263334 AW098620 similar to TR:Q9ZRI8 Q9ZRI8 FORMATE DEHYDROGENASE 606 0...”
Q8GUA8 Ornithine aminotransferase from Medicago truncatula
36% identity, 79% coverage
KR75_05020 putrescine aminotransferase from Klebsiella variicola
36% identity, 80% coverage
- Molecular mechanism of endophytic bacteria DX120E regulating polyamine metabolism and promoting plant growth in sugarcane
Qin, Frontiers in plant science 2024 - “...4567649-4568509 KR75_20310 spermidine acetyltransferase speG 2908943-2909503 Degradation and transporters KR75_04635 bifunctional glutathionylspermidine amidase/synthase gss 712705-714570 KR75_05020 putrescine aminotransferase ygjG 633588-634967 KR75_09810 Fe 3+ /spermidine/putrescine ABC transporter ATP-binding protein 5129965-5130996 KR75_09825 spermidine/putrescine ABC transporter permease 5127048-5127905 KR75_09940 spermidine/putrescine ABC transporter substrate-binding protein 5101970-5102389 KR75_16570 spermidine/putrescine ABC transporter...”
VP1771 4-aminobutyrate aminotransferase from Vibrio parahaemolyticus RIMD 2210633
34% identity, 89% coverage
RL0102 putative 4-aminobutyrate aminotransferase from Rhizobium leguminosarum bv. viciae 3841
38% identity, 85% coverage
- BacA is essential for bacteroid development in nodules of galegoid, but not phaseoloid, legumes
Karunakaran, Journal of bacteriology 2010 - “...category is the gene encoding 4-aminobutyrate transferase gabT (RL0102), which was highly elevated (35-fold, P 0.001) in 7-day bacteroids in 3841 (22, 37) but...”
- Pathway of gamma-aminobutyrate metabolism in Rhizobium leguminosarum 3841 and its role in symbiosis
Prell, Journal of bacteriology 2009 - “...The GABA:2-oxoglutarate aminotransferase gene was annotated as gabT (RL0102) because of its identity to the previously identified gabT gene in R. leguminosarum...”
- “...AMS plus glucose and NH4Cl (AMS-glucose-NH4Cl). The gabT gene (RL0102) was induced by GABA 7.6-fold (P value of 0.03), and the gabD2 gene was induced by GABA...”
- Transcriptomic analysis of Rhizobium leguminosarum biovar viciae in symbiosis with host plants Pisum sativum and Vicia cracca
Karunakaran, Journal of bacteriology 2009 - “...in -aminobutyrate metabolism, gabD2 (RL0101) and gabT (RL0102), were induced two- and sevenfold, respectively. However, both were more strongly elevated at...”
- “...pRL90031 pRL90039 pRL90046 pRL90047b pRL90194 pRL90322 RL0018b RL0054b RL0102 RL0540 RL0680b RL0702 RL0913b Gene name metX thiC fdxB nifN nifE nifK nifD nifH...”
XP_015085834 ornithine aminotransferase, mitochondrial isoform X2 from Solanum pennellii
36% identity, 79% coverage
RSc0029 PROBABLE 4-AMINOBUTYRATE AMINOTRANSFERASE PROTEIN from Ralstonia solanacearum GMI1000
37% identity, 84% coverage
- The GABA transaminase GabT is required for full virulence of Ralstonia solanacearum in tomato
Xian, microPublication biology 2021 - “...1A ). The genome of R. solanacearum contains a single copy of the gabT gene (RSc0029), which is located upstream of the gabD gene (RSc0028) in the same operon ( Figure 1B ). Interestingly, a gabT knockout mutant was unable to use GABA as a nutrient,...”
- “...To complement gabT in the gabT mutant (Xian et al. , 2020), the gabT gene (RSc0029) with its promoter (300 bp upstream of ATG of the gabT gene) was amplified by PCR, cloned into pENTR-TOPO, and then introduced into pRCT-GWY (Monteiro et al. , 2012) by...”
VpaChn25_1376 4-aminobutyrate--2-oxoglutarate transaminase from Vibrio parahaemolyticus
34% identity, 89% coverage
- Genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (VpaCspA and VpaCspD) in Vibrio parahaemolyticus CHN25 during low-temperature survival
Zhu, BMC genomics 2017 - “...dehydrogenase VpaChn25A_0565 7.3736 3-hydroxyisobutyrate dehydrogenase VpaChn25A_1033 2.7479 Methylmalonate-semialdehyde dehydrogenase VpaChn25A_1036 2.1029 Acyl-CoA dehydrogenase Propanoate metabolism VpaChn25_1376 6.0834 4-aminobutyrate aminotransferase VpaChn25_1635 6.3526 PrpE protein VpaChn25_1638 8.5787 Methylcitrate synthase VpaChn25_1639 6.7065 2-methylisocitrate lyase VpaChn25_2798 3.959 Acetyl-CoA synthetase ABC transporters VpaChn25A_0128 0.4376 ABC transporter substrate-binding protein VpaChn25A_0130 0.428 ABC...”
- “...2.1782 Glutamate synthase subunit beta VpaChn25_0437 2.0222 Glutamate synthase subunit alpha VpaChn25_1375 3.4554 Succinate-semialdehyde dehydrogenase VpaChn25_1376 3.0672 4-aminobutyrate aminotransferase VpaChn25_2552 0.4804 Glutaminase VpaChn25_2583 2.0986 Aspartate carbamoyltransferase VpaChn25_2584 2.7419 Aspartate carbamoyltransferase VpaChn25_2784 0.4744 Aspartate ammonia-lyase Arginine and proline metabolism VpaChn25_1371 2.2205 Aldehyde dehydrogenase VpaChn25_1372 2.779 Oxidoreductase VpaChn25_1373...”
A0A5B8KQ12 4-aminobutyrate-2-oxoglutarate transaminase (EC 2.6.1.19) from Agrobacterium tumefaciens (see paper)
37% identity, 85% coverage
WP_042715413 putrescine aminotransferase from Enterobacter mori
36% identity, 80% coverage
- The scale-free nature of protein sequence space
Buchholz, PloS one 2018 - “...Proteobacteria WP_001303675 259 DC pyruvate dehydrogenase subunit Gammaproteo-bacteria WP_044256366 266 oTA putrescine aminotransferase Enterobacter cloacae WP_042715413 381 aspartate aminotransferase Shigella WP_000069444 378 SDR GDP-mannose 4,6-dehydratase Helicobacter pylori WP_058338748 312 Dimensions of protein sequence networks As a further network property, the fractal network dimension D f was...”
An04g04130 uncharacterized protein from Aspergillus niger
33% identity, 81% coverage
- The International Space Station Environment Triggers Molecular Responses in Aspergillus niger
Blachowicz, Frontiers in microbiology 2022 - “...1.11 5.81E-03 An16g07920 Hypothetical protein 1.08 2.87E-02 An04g01230 EcmA Cell wall organization protein 1.02 8.38E-04 An04g04130 Ornithine transaminase 1.03 1.31E-02 An16g07110 Ach1 Acetyl-CoA hydrolase 1.06 2.25E-02 An04g04870 Superoxide dismutase 1.08 4.71E-03 An01g03740 XyrA D-xylose reductase 1.08 2.59E-02 An07g08990 PkiA Pyruvate kinase 1.12 2.92E-03 An08g00970 Rps28 Ribosomal...”
- “...-value An01g14960 Asparaginase 2.21 6.46E-04 An12g00160 Malate dehydrogenase 1.27 2.72E-03 An14g06010 Chorismate mutase 1.01 1.50E-02 An04g04130 Ornithine transaminase 1.03 1.31E-02 An16g02970 Glycine / Serine hydroxymethyltransferase 1.05 2.74E-04 An11g09510 Aspartate semialdehyde dehydrogenase 1.06 2.35E-03 An01g06530 Branched-chain-amino acid transaminase activity 1.18 2.49E-02 An15g05770 Hydrogen sulfide / sulfur amino...”
Echvi_0577 Ornithine aminotransferase (EC 2.6.1.13) from Echinicola vietnamensis KMM 6221, DSM 17526
34% identity, 90% coverage
- mutant phenotype: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (ORNITHINE-GLU-AMINOTRANSFERASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 789,361 different protein sequences to 1,256,019 scientific articles. Searches against EuropePMC were last performed on January 10 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory