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PaperBLAST

PaperBLAST Hits for SwissProt::A0A0H2VAP9 D-serine transporter DsdX; D-serine-specific permease (Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)) (445 a.a., MHSQIWVVST...)

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Find functional residues: SitesBLAST

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Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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Found 77 similar proteins in the literature:

DSDX_ECOL6 / A0A0H2VAP9 D-serine transporter DsdX; D-serine-specific permease from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) (see paper)
c2900 DsdX permease from Escherichia coli CFT073
100% identity, 100% coverage

UTI89_C4956 hypothetical protein from Escherichia coli UTI89
99% identity, 100% coverage

DsdC / b2365 D-serine transporter from Escherichia coli K-12 substr. MG1655 (see 4 papers)
DSDX_ECOLI / P08555 D-serine transporter DsdX from Escherichia coli (strain K12) (see 2 papers)
TC 2.A.8.1.5 / P08555 The D-serine transporter, DsdX (KM=60µM) (may also transport D-threonine which inhibits D-serine uptake) (Anfora and Welch, 2006). Eliminating the dsdX gene renders the cell more sensitive to chloramphenicol from Escherichia coli (strain K12) (see 9 papers)
b2365 predicted transporter from Escherichia coli str. K-12 substr. MG1655
BW25113_2365 D-serine transporter DsdX from Escherichia coli BW25113
99% identity, 100% coverage

STM3801 putative Gnt family transport protein from Salmonella typhimurium LT2
SEEHRA37_02334 D-serine transporter DsdX from Salmonella enterica subsp. enterica serovar Heidelberg str. SARA37
94% identity, 100% coverage

PAU_02599 dsdx permease from Photorhabdus asymbiotica
81% identity, 100% coverage

PMI0186 DsdX permease from Proteus mirabilis HI4320
79% identity, 100% coverage

HWQ47_08850 D-serine transporter DsdX from Shewanella sp. MTB7
75% identity, 100% coverage

PfGW456L13_3649 D-serine permease from Pseudomonas fluorescens GW456-L13
49% identity, 97% coverage

RR42_RS28835 gluconate:H+ symporter (gntT) from Cupriavidus basilensis FW507-4G11
40% identity, 93% coverage

BCAL3365 putative gluconate permease from Burkholderia cenocepacia J2315
43% identity, 96% coverage

AbA118F_1623 gluconate:H+ symporter from Acinetobacter baumannii
42% identity, 96% coverage

ACIAD0544 high-affinity gluconate permease (GntP family) from Acinetobacter sp. ADP1
41% identity, 98% coverage

PA14_34630 gluconate permease from Pseudomonas aeruginosa UCBPP-PA14
PA2322 gluconate permease from Pseudomonas aeruginosa PAO1
39% identity, 99% coverage

YPO3954 putative gluconate permease from Yersinia pestis CO92
37% identity, 97% coverage

PP3417, PP_3417 gluconate transporter from Pseudomonas putida KT2440
39% identity, 96% coverage

ZMO1756 gluconate transporter from Zymomonas mobilis subsp. mobilis ZM4
36% identity, 95% coverage

SA2293 gluconate permease from Staphylococcus aureus subsp. aureus N315
SAUSA300_2442 gluconate permease from Staphylococcus aureus subsp. aureus USA300_FPR3757
SACOL2514 gluconate transporter, permease protein from Staphylococcus aureus subsp. aureus COL
SA_RS13150 gluconate:H+ symporter from Staphylococcus aureus subsp. aureus N315
36% identity, 98% coverage

SERP2057 gluconate transporter, permease protein from Staphylococcus epidermidis RP62A
SE2044 gluconate permease from Staphylococcus epidermidis ATCC 12228
36% identity, 98% coverage

CH51_RS14180 gluconate:H+ symporter from Staphylococcus aureus
36% identity, 98% coverage

BPHYT_RS16725 gluconate:H+ symporter (gntT) from Burkholderia phytofirmans PsJN
39% identity, 91% coverage

YhgC / b3415 high-affinity gluconate transporter from Escherichia coli K-12 substr. MG1655 (see 5 papers)
gntT / P39835 high-affinity gluconate transporter from Escherichia coli (strain K12) (see 4 papers)
GNTT_ECOLI / P39835 High-affinity gluconate transporter; Gluconate permease; Gnt-I system from Escherichia coli (strain K12) (see paper)
TC 2.A.8.1.4 / P39835 D-Gluconate:H+ symporter, GntT from Escherichia coli (see 9 papers)
A0A140N385 Gluconate transporter from Escherichia coli (strain B / BL21-DE3)
b3415 gluconate transporter, high-affinity GNT I system from Escherichia coli str. K-12 substr. MG1655
37% identity, 97% coverage

Z4770 high-affinity transport of gluconate / gluconate permease from Escherichia coli O157:H7 EDL933
37% identity, 97% coverage

TC 2.A.8.1.1 / P12012 D-Gluconate:H+ symporter of 448 aas and 11 TMSs from Bacillus subtilis (see 3 papers)
37% identity, 97% coverage

BWI76_RS26615 gluconate:H+ symporter from Klebsiella michiganensis M5al
37% identity, 97% coverage

STM3512 GntP family, high-affinity gluconate permease in GNT I system from Salmonella typhimurium LT2
37% identity, 97% coverage

SXYL_00159 gluconate:H+ symporter from Staphylococcus xylosus
38% identity, 98% coverage

CA_C3605 gluconate:H+ symporter from Clostridium acetobutylicum ATCC 824
35% identity, 98% coverage

SAR2582 putative gluconate permease from Staphylococcus aureus subsp. aureus MRSA252
36% identity, 98% coverage

GOX2188 Gluconate permease from Gluconobacter oxydans 621H
37% identity, 95% coverage

GBAA3429 gluconate permease from Bacillus anthracis str. 'Ames Ancestor'
37% identity, 98% coverage

BAS0165 gluconate permease from Bacillus anthracis str. Sterne
BA0163 gluconate permease from Bacillus anthracis str. Ames
AW20_2625 gluconate permease GntP from Bacillus anthracis str. Sterne
36% identity, 94% coverage

RHA1_ro02363 gluconate permease family protein from Rhodococcus sp. RHA1
37% identity, 94% coverage

PVLB_13665 GntP family permease from Pseudomonas sp. VLB120
39% identity, 96% coverage

STM4482 GntP family, L-idonate transport protein from Salmonella typhimurium LT2
33% identity, 97% coverage

YjgT / b4265 L-idonate/5-ketogluconate/gluconate transporter from Escherichia coli K-12 substr. MG1655 (see paper)
idnT / P39344 L-idonate/5-ketogluconate/gluconate transporter from Escherichia coli (strain K12) (see 5 papers)
IDNT_ECOLI / P39344 Gnt-II system L-idonate transporter; L-Ido transporter; L-idonate transporter; 5-keto-D-gluconate transporter; L-idonate/5-ketogluconate/gluconate transporter from Escherichia coli (strain K12) (see 5 papers)
TC 2.A.8.1.2 / P39344 L-Idonate/D-Gluconate:H+ symporter, IdnT, GntW, YjgT from Escherichia coli (see 5 papers)
b4265 L-idonate and D-gluconate transporter from Escherichia coli str. K-12 substr. MG1655
33% identity, 95% coverage

SR35_21910 gnt-II system L-idonate transporter from Escherichia coli
33% identity, 95% coverage

SCO1680 gluconate permease from Streptomyces coelicolor A3(2)
36% identity, 95% coverage

SXYL_02351 gluconate:H+ symporter from Staphylococcus xylosus
36% identity, 92% coverage

U876_22450 gluconate transporter from Aeromonas hydrophila NJ-35
36% identity, 95% coverage

llmg_2467 gluconate transport protein from Lactococcus lactis subsp. cremoris MG1363
33% identity, 90% coverage

BKKJ1_0207 GntP family permease from Bifidobacterium catenulatum subsp. kashiwanohense
35% identity, 94% coverage

YPTB3287 putative GntP family gluconate transporter from Yersinia pseudotuberculosis IP 32953
32% identity, 99% coverage

YjiB / b4321 fructuronate transporter from Escherichia coli K-12 substr. MG1655 (see 6 papers)
gntP / P0AC94 fructuronate transporter from Escherichia coli (strain K12) (see 6 papers)
TC 2.A.8.1.3 / P0AC94 D-Fructuronate/D-gluconate:H+ symporter, GntP from Escherichia coli (see 5 papers)
b4321 fructuronate transporter from Escherichia coli str. K-12 substr. MG1655
NP_418741 fructuronate transporter from Escherichia coli str. K-12 substr. MG1655
Z5919 gluconate transport system permease 3 from Escherichia coli O157:H7 EDL933
ETEC_4633 gluconate permease GntP from Escherichia coli ETEC H10407
31% identity, 98% coverage

PP0652, PP_0652 gluconate transporter from Pseudomonas putida KT2440
31% identity, 91% coverage

YjhF / b4296 KpLE2 phage-like element; putative transporter YjhF from Escherichia coli K-12 substr. MG1655 (see 4 papers)
33% identity, 93% coverage

PPUBIRD1_0697 GntP family permease from Pseudomonas putida BIRD-1
32% identity, 88% coverage

SCO4991 sugar transport integral membrane protein from Streptomyces coelicolor A3(2)
35% identity, 84% coverage

LKW31_01605 gluconate transporter from Pantoea agglomerans
37% identity, 95% coverage

GntU / b4476 low-affinity gluconate transporter from Escherichia coli K-12 substr. MG1655 (see 4 papers)
gntU / P0AC96 low-affinity gluconate transporter from Escherichia coli (strain K12) (see 4 papers)
TC 2.A.8.1.8 / P0AC96 Low-affinity (212 from Escherichia coli (strain K12) (see 7 papers)
Z4804 low affinity gluconate permease from Escherichia coli O157:H7 EDL933
35% identity, 95% coverage

STM3541 low affinity gluconate permease from Salmonella typhimurium LT2
35% identity, 98% coverage

PFREUD_22850 gluconate:H+ symporter from Propionibacterium freudenreichii subsp. shermanii CIRM-BIA1
33% identity, 94% coverage

STM2913 putative permease from Salmonella typhimurium LT2
33% identity, 87% coverage

STM14_3515 GntP family permease from Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
33% identity, 87% coverage

SEEHRA37_18800 GntP family permease from Salmonella enterica subsp. enterica serovar Heidelberg str. SARA37
33% identity, 87% coverage

MSMEG_3789 inner membrane permease YgbN from Mycobacterium smegmatis str. MC2 155
31% identity, 94% coverage

HI1015 gluconate permease (gntP) from Haemophilus influenzae Rd KW20
33% identity, 84% coverage

PMI2672 low-affinity gluconate transporter from Proteus mirabilis HI4320
31% identity, 99% coverage

SO1771 permease, GntP family from Shewanella oneidensis MR-1
33% identity, 95% coverage

FN0554 D-serine permease from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
32% identity, 94% coverage

APL_1665 gluconate permease from Actinobacillus pleuropneumoniae L20
31% identity, 96% coverage

cg3216 gluconate permease from Corynebacterium glutamicum ATCC 13032
31% identity, 89% coverage

VCA0904 permease from Vibrio cholerae O1 biovar eltor str. N16961
34% identity, 95% coverage

YgbN / b2740 putative transporter YgbN from Escherichia coli K-12 substr. MG1655 (see 4 papers)
b2740 predicted transporter from Escherichia coli str. K-12 substr. MG1655
Q46892 Inner membrane permease YgbN from Escherichia coli (strain K12)
30% identity, 92% coverage

PA1051 probable transporter from Pseudomonas aeruginosa PAO1
PA14_50770 probable transporter from Pseudomonas aeruginosa UCBPP-PA14
32% identity, 95% coverage

ESA_02715 hypothetical protein from Enterobacter sakazakii ATCC BAA-894
33% identity, 89% coverage

ESA_02715 GntP family permease from Cronobacter sakazakii ATCC BAA-894
33% identity, 90% coverage

NMV_2230, NMY220_1858 GntP family permease from Neisseria meningitidis 8013
30% identity, 92% coverage

VC0286 gluconate permease, putative from Vibrio cholerae O1 biovar eltor str. N16961
30% identity, 92% coverage

Q7UET7 Gluconate permease gntP from Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)
26% identity, 86% coverage

NJ7G_3288 GntP family permease from Natrinema sp. J7-2
27% identity, 92% coverage

Q7UNE5 Probable Gnt-II system L-idonate transporter from Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)
31% identity, 37% coverage

VSAL_I2593 gluconate permease from Vibrio salmonicida LFI1238
VSAL_I2593 GntP family permease from Aliivibrio salmonicida LFI1238
26% identity, 56% coverage

VV1_1655 H+/gluconate symporter from Vibrio vulnificus CMCP6
26% identity, 51% coverage

MA0021 high-affinity gluconate transporter (gluconate permease) from Methanosarcina acetivorans C2A
28% identity, 36% coverage

CPE0860 probable gluconate permease from Clostridium perfringens str. 13
21% identity, 80% coverage

New Search

For advice on how to use these tools together, see Interactive tools for functional annotation of bacterial genomes.

Statistics

The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.

How It Works

PaperBLAST builds a database of protein sequences that are linked to scientific articles. These links come from automated text searches against the articles in EuropePMC and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot, BRENDA, CAZy (as made available by dbCAN), BioLiP, CharProtDB, MetaCyc, EcoCyc, TCDB, REBASE, the Fitness Browser, and a subset of the European Nucleotide Archive with the /experiment tag. Given this database and a protein sequence query, PaperBLAST uses protein-protein BLAST to find similar sequences with E < 0.001.

To build the database, we query EuropePMC with locus tags, with RefSeq protein identifiers, and with UniProt accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use queries of the form "locus_tag AND genus_name" to try to ensure that the paper is actually discussing that gene. Because EuropePMC indexes most recent biomedical papers, even if they are not open access, some of the links may be to papers that you cannot read or that our computers cannot read. We query each of these identifiers that appears in the open access part of EuropePMC, as well as every locus tag that appears in the 500 most-referenced genomes, so that a gene may appear in the PaperBLAST results even though none of the papers that mention it are open access. We also incorporate text-mined links from EuropePMC that link open access articles to UniProt or RefSeq identifiers. (This yields some additional links because EuropePMC uses different heuristics for their text mining than we do.)

For every article that mentions a locus tag, a RefSeq protein identifier, or a UniProt accession, we try to select one or two snippets of text that refer to the protein. If we cannot get access to the full text, we try to select a snippet from the abstract, but unfortunately, unique identifiers such as locus tags are rarely provided in abstracts.

PaperBLAST also incorporates manually-curated protein functions:

Except for GeneRIF and ENA, the curated entries include a short curated description of the protein's function. For entries from BioLiP, the protein's function may not be known beyond binding to the ligand. Many of these entries also link to articles in PubMed.

For more information see the PaperBLAST paper (mSystems 2017) or the code. You can download PaperBLAST's database here.

Changes to PaperBLAST since the paper was written:

Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.

Secrets

PaperBLAST cannot provide snippets for many of the papers that are published in non-open-access journals. This limitation applies even if the paper is marked as "free" on the publisher's web site and is available in PubmedCentral or EuropePMC. If a journal that you publish in is marked as "secret," please consider publishing elsewhere.

Omissions from the PaperBLAST Database

Many important articles are missing from PaperBLAST, either because the article's full text is not in EuropePMC (as for many older articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an article that characterizes a protein's function but is missing from PaperBLAST, please notify the curators at UniProt or add an entry to GeneRIF. Entries in either of these databases will eventually be incorporated into PaperBLAST. Note that to add an entry to UniProt, you will need to find the UniProt identifier for the protein. If the protein is not already in UniProt, you can ask them to create an entry. To add an entry to GeneRIF, you will need an NCBI Gene identifier, but unfortunately many prokaryotic proteins in RefSeq do not have corresponding Gene identifers.

References

PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.

Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.

Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.

UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.

BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.

The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.

The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.

CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.

The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.

The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.

REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.

Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory