PaperBLAST
PaperBLAST Hits for SwissProt::D4GYE4 PTS system fructose-specific EIIA component; EIIA-Fru; Fructose-specific phosphotransferase enzyme IIA component (Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)) (154 a.a., MDVTDISTIT...)
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>SwissProt::D4GYE4 PTS system fructose-specific EIIA component; EIIA-Fru; Fructose-specific phosphotransferase enzyme IIA component (Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii))
MDVTDISTITPLELISLEEPPATKEGAIEFLLDLAVDAGRVDDRDAALDALLEREGEATT
GVGFGIGIPHAKTDAVSKPTVAFARSAEGIDFDAMDDKPAKLLFMILVPAAGGEDHLQIL
SALSRSLMHEDVREKLLEAESKQTVQDVLAEVVE
Running BLASTp...
Found 239 similar proteins in the literature:
PTFA_HALVD / D4GYE4 PTS system fructose-specific EIIA component; EIIA-Fru; Fructose-specific phosphotransferase enzyme IIA component from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
TC 4.A.2.1.15 / D4GYE4 Sugar phosphotransferase system IIA component, component of Fructose-specific PTS, PtfABC from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (see paper)
HVO_1498 sugar hosphotransferase system IIA component from Haloferax volcanii DS2
100% identity, 100% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II PtfABC PTS system is involved in fructose transport.
- substrates: fructose
tcdb comment: The transcriptional regulation of the fructose PTS in the very similar organism, Haloferax mediterranei, has shown that GlpR is a transcriptional activator (Cai et al. 2014) - Fructose degradation in the haloarchaeon Haloferax volcanii involves a bacterial type phosphoenolpyruvate-dependent phosphotransferase system, fructose-1-phosphate kinase, and class II fructose-1,6-bisphosphate aldolase
Pickl, Journal of bacteriology 2012 - “...EIIB (encoded by HVO_1496 [ptsI], HVO_1497 [ptsH1], HVO_1498 [ptfA], and HVO_1495 [ptfB], respectively) and the transmembrane component EIIC (encoded by...”
TC 4.A.2.1.14 / D2RXA7 Putative PTS IIA-like nitrogen-regulatory protein PtsN, component of Fructose Enzyme II complex (IIAFru - IIBFru - IICFru) (based on homology) from Haloterrigena turkmenica (strain ATCC 51198 / DSM 5511 / NCIMB 13204 / VKM B-1734) (see paper)
69% identity, 94% coverage
TC 4.A.2.1.26 / Q5V5X4 Sugar hosphotransferase system IIA component, component of Fructose-specific Enzyme I-HPr-Enzyme IIABC complex, all encoded within a single operon with genes in the order: ptsC (IIC), ptsA (IIA), ptsH (HPr), ptsI (Enzyme I) and ptsB (IIB) from Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
68% identity, 94% coverage
Halsa_0019 PTS sugar transporter subunit IIA from Halanaerobium hydrogeniformans
44% identity, 94% coverage
- Going from microbial ecology to genome data and back: studies on a haloalkaliphilic bacterium isolated from Soap Lake, Washington State
Mormile, Frontiers in microbiology 2014 - “...fructose ( FruA ) are also present. Halanaerobium hydrogeniformans has two putative nitrogen-related PTS genes, Halsa_0019 and Halsa_2283. Nitrogen-related PTS genes are found in Gram-negative bacteria, can regulate carbon and nitrogen metabolism, are required for virulence by some bacteria, and can play a role in potassium...”
- “...In addition, when a BLAST search was performed on the amino acid sequence encoded by Halsa_0019, a 79 and 76% identity was found with a fructose-specific PTS from Halanaerobium saccharolyticum and Halanaerobium praevalens , respectively. The role for Halsa_2283 isnt as apparent as for Halsa_0019. The...”
TKV_c23140 PTS sugar transporter subunit IIA from Thermoanaerobacter kivui
46% identity, 79% coverage
SBI_04453 fructose-specific PTS transporter subunit EIIC from Streptomyces bingchenggensis BCW-1
52% identity, 18% coverage
Csac_2440 putative PTS IIA-like nitrogen-regulatory protein PtsN from Caldicellulosiruptor saccharolyticus DSM 8903
42% identity, 89% coverage
CAC0233 PTS system, IIA component from Clostridium acetobutylicum ATCC 824
45% identity, 84% coverage
- Proteomic analyses of the phase transition from acidogenesis to solventogenesis using solventogenic and non-solventogenic Clostridium acetobutylicum strains
Jang, Applied microbiology and biotechnology 2014 (PubMed)- “...phase. Of these, four increased proteins (AdhE1, CAC0233, CtfB and phosphocarrier protein HPr) and six decreased proteins (butyrate kinase, ferredoxin:pyruvate...”
- “...whereas the levels of a fructose-specific PTS IIA (CAC0233) were elevated. Interestingly, three protein spots corresponding to HPr were barely observed during...”
- DENSE: efficient and prior knowledge-driven discovery of phenotype-associated protein functional modules
Hendrix, BMC systems biology 2011 - “...by DENSE is presented. Proteins involved in this cluster include E1 proteins (CAC0231), EII enzymes (CAC0233 and CAC0234), a transcriptional regulator involved in sugar metabolism (CAC0231), and fructose 1-phosphate kinase (CAC0232). The EII proteins and fructose 1-phosphate kinase are shown to interact with each protein in...”
- “...5 Description of acid tolerent cluster identified by DENSE STRING ID Protein ID Protein Description CAC0233 - PTS system, IIA component CAC0231 - Transcriptional regulator of sugar metabolism CAC3087 - Phosphoenolpyruvate-protein kinase (PTS system enzyme I) CAC0232 - 1-phosphofructokinase (fructose 1-phosphate kinase) CAC0234 - PTS system...”
- A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat culture
Janssen, Applied microbiology and biotechnology 2010 - “...12.1 3.7 5.5 4.4 K,G CAC0232 fruB 1-phosphofructokinase 2.2 1.9 11.8 2.9 4.7 4.8 G CAC0233 PTS system, IIA component 2.5 2.0 18.2 2.3 6.2 8.0 G,T CAC0234 PTS system, fructoso-specific IIBC component 2.2 2.1 8.1 3.0 3.9 2.9 G CAC0360 Transcriptional regulator 3.1 2.6 6.5...”
B5X54_RS01270 PTS sugar transporter subunit IIA from Caldicellulosiruptor bescii
41% identity, 92% coverage
- Gene targets for engineering osmotolerance in Caldicellulosiruptor bescii
Sander, Biotechnology for biofuels 2020 - “...conditions Locus tag Annotation log 2 (ORCB002/JWCB005) Glycerol Glucose NaCl B5X54_RS01265 1-Phosphofructokinase 4.2 0.8 4.5 B5X54_RS01270 PTS fructose transporter subunit IIA 4.5 0.7 4.5 B5X54_RS01275 PTS fructose transporter subunit IIBC 4.8 0.6 4.3 B5X54_RS01280 HPr family phosphocarrier protein 4.1 0.8 3.7 B5X54_RS01285 Phosphoenolpyruvate-protein phosphotransferase 4.5 0.6...”
BAGQ_1558 PTS fructose transporter subunit IIABC from Bacillus velezensis
44% identity, 20% coverage
TC 4.A.2.1.4 / P71012 The fructose porter, FruA (fructose-1-P forming IIABC) (Delobbe et al. 1975) FruA is 39% identical to 4.A.2.1.1). fructose can be metabolized to Fru-1-P via this system as well as Fru-6-P by another PTS system from Bacillus subtilis (see paper)
43% identity, 20% coverage
D8ID87 PTS system, fructose-specific, IIABC component from Brachyspira pilosicoli (strain ATCC BAA-1826 / 95/1000)
37% identity, 24% coverage
- The Exposed Proteomes of Brachyspira hyodysenteriae and B. pilosicoli
Casas, Frontiers in microbiology 2016 - “...system glucose subfamily IIA subunit 90 D8IBR5 ptsG BRAPL PTS system fructose-specific IIABC component 88 D8ID87 fruA BRAPL Transcriptional regulator XRE family 86 J9TSW8 B2904_orf609 BRAPL PTS system fructose specific transporter subunit IIABC 83 K0JLH4 WESB_2470 BRAPL Methyl-accepting protein 80 D8IBS9 , C0R060 BP951000_0603, tar5 C...”
WESB_2470 fructose-specific PTS transporter subunit EIIC from Brachyspira pilosicoli WesB
K0JLH4 PTS system fructose-specific transporter subunit IIABC from Brachyspira pilosicoli WesB
36% identity, 24% coverage
- The Exposed Proteomes of Brachyspira hyodysenteriae and B. pilosicoli
Casas, Frontiers in microbiology 2016 - “...XRE family 86 J9TSW8 B2904_orf609 BRAPL PTS system fructose specific transporter subunit IIABC 83 K0JLH4 WESB_2470 BRAPL Methyl-accepting protein 80 D8IBS9 , C0R060 BP951000_0603, tar5 C Unchar J9UB61 79 J9UB61 B2904_orf310 BRAPL Transcriptional regulator CarD family 71 D8ID15 carD BRAPL * The members column lists protein...”
- The Exposed Proteomes of Brachyspira hyodysenteriae and B. pilosicoli
Casas, Frontiers in microbiology 2016 - “...regulator XRE family 86 J9TSW8 B2904_orf609 BRAPL PTS system fructose specific transporter subunit IIABC 83 K0JLH4 WESB_2470 BRAPL Methyl-accepting protein 80 D8IBS9 , C0R060 BP951000_0603, tar5 C Unchar J9UB61 79 J9UB61 B2904_orf310 BRAPL Transcriptional regulator CarD family 71 D8ID15 carD BRAPL * The members column lists...”
C289_1174 PTS fructose transporter subunit IIABC from Anoxybacillus ayderensis
41% identity, 24% coverage
- A high molecular-mass Anoxybacillus sp. SK3-4 amylopullulanase: characterization and its relationship in carbohydrate utilization
Kahar, International journal of molecular sciences 2013 - “...Several putative sugar transporters (C289_0465, C289_0466, C289_0467, C289_0603, C289_0763, C289_0764, C289_0765, C289_0778, C289_0779, C289_0780, C289_1015, C289_1174, C289_1392, C289_1394, C289_1910, C289_1911, and C289_1912) were also found in the Blast2GO annotation. The draft genome was submitted to the National Center for Biotechnology Information (NCBI) Bioproject with accession no....”
OG1RF_10454 fructose-specific PTS transporter subunit EIIC from Enterococcus faecalis OG1RF
EF0717 PTS system, fructose-specific family, IIABC components from Enterococcus faecalis V583
45% identity, 19% coverage
- Global Regulation of Gene Expression by the MafR Protein of Enterococcus faecalis
Ruiz-Cruz, Frontiers in microbiology 2015 - “...2 FC of mutant strain versus wild-type strain was about 4). The latter genes were OG1RF_10454 ( fruA , PTS family fructose porter, IIABC component), OG1RF_10455 ( fruk2 , 1-phosphofructokinase), and OG1RF_10456 ( lacR , lactose PTS family porter repressor), which constitute an operon ( lacR-fruk2-fruA...”
- The Intraperitoneal Transcriptome of the Opportunistic Pathogen Enterococcus faecalis in Mice
Muller, PloS one 2015 - “...(EF0709 and EF0710, respectively) are significantly downregulated (-4.9-fold) as well as a fructose specific PTS (EF0717; -15.7-fold) ( S4 Table and Fig 2 ). This suggests that PTS sugars may be scarce in the mouse peritoneum, a conclusion supported by the fact that expression of only...”
- The transcriptome of the nosocomial pathogen Enterococcus faecalis V583 reveals adaptive responses to growth in blood
Vebø, PloS one 2009 - “...during growth in blood and mannitol (EF0411-12) during growth in YTB. The fructose PTS system (EF0717), was up-regulated in blood, but down-regulated in YTB. The PTS mannose/fructose/sorbose (EF3029) showed a reduced expression during growth in both blood and YTB. The observed changes in substrate utilization and...”
- Survey of genomic diversity among Enterococcus faecalis strains by microarray-based comparative genomic hybridization
Aakra, Applied and environmental microbiology 2007 - “...carbohydrates ORF (gene) EF1131 (araD) EF1159 EF1160 EF0694 EF0717 EF1167 (fba) EF1504 EF1834 (lacB) EF1835 (lacA) EF1416 (pgi) EF1129 EF1928 EF1929 (glpK)...”
- Transcriptional response of Enterococcus faecalis V583 to erythromycin
Aakra, Antimicrobial agents and chemotherapy 2005 - “...EF1645 EF2066 EF2259 EF2653 EF2852 EF3272 EF0021 EF0022 EF0717 EF0958 EF1051 EF1261 EF1516 EF2978 EF2979 EF0846 EF1170 EF3214 EF0188 EF0636 EF0744 EF0760 EF0789...”
SE0472 PTS system fructose-specific IIABC component from Staphylococcus epidermidis ATCC 12228
F1613_RS05160 PTS fructose transporter subunit IIABC from Staphylococcus epidermidis
40% identity, 23% coverage
- Identification of antigenic components of Staphylococcus epidermidis expressed during human infection
Pourmand, Infection and immunity 2006 - “...hypothetical protein Dehydrogenase 40 465589-467653 SE0472 (591-650) SE0473 (1-390) Phosphotransferase system fructose-specific IIABC component...”
- Genome-wide transcription response of Staphylococcus epidermidis to heat shock and medically relevant glucose levels
Benjamin, Frontiers in microbiology 2024 - “...kinase), lacB , and lacA ), ribose transport ( rbsU , rbsD ), fructose utilization (F1613_RS05160 (PTS fructose transporter subunit IIABC), pfkB , and F1613_RS05150 (DeoR/GlpR family DNA-binding transcription regulator)), proline catabolism (F1613_RS09950 (proline dehydrogenase)), the glyoxalase pathway (F1613_RS05685 (glyoxalase)), the succinate dehydrogenase complex (F1613_RS07025 (succinate...”
- “...role in the utilization of fructose, (F1613_RS05150 (DeoR/GlpR family DNA-binding transcription regulator), pfkB , and F1613_RS05160 (PTS fructose transporter subunit IIABC)) ( Ge et al., 2024 ; Supplementary Table S6 ). We found that L-serine ammonia-lyase ironsulfur-dependent subunits alpha and beta ( sdaAA and sdaAB ),...”
SA0655 fructose specific permease from Staphylococcus aureus subsp. aureus N315
SAV0700 fructose specific permease from Staphylococcus aureus subsp. aureus Mu50
45% identity, 20% coverage
- Sulfide Homeostasis and Nitroxyl Intersect via Formation of Reactive Sulfur Species in Staphylococcus aureus
Peng, mSphere 2017 - “...ATP:guanido phosphotransferase NWMN_0487 SA0483 clpC 12.7 ClpC protease, ATP-binding subunit NWMN_0587 SA0572 15.5 Hypothetical NWMN_0669 SA0655 fruA 14.2 Fructose permease (NWMN_06670669, frc operon) NWMN_0815 SA0806 17.5 8.4 NADH-dependent pyridine nucleotide disulfide oxidoreductase (short-chain dehydrogenase/reductase [SDR]) Yes (229) NWMN_0826 SA0817 13.1 NADH-dependent flavin oxidoreductase NWMN_0845 SA0835 28.2...”
- Global transcriptome analysis of Staphylococcus aureus biofilms in response to innate immune cells
Scherr, Infection and immunity 2013 - “...SA1182 SA1435 SA1364 SA2553 SA1814 SA2303 SA1693 SA0655 SA1109 SA2635 SA1844 SA0308 SA2195 SA0921 SA1876 SA1309 Rhodanese-like domain protein L-Lactate permease...”
- Transcriptional signature following inhibition of early-stage cell wall biosynthesis in Staphylococcus aureus
O'Neill, Antimicrobial agents and chemotherapy 2009 - “...SA0268 SA0269 SA0270 SA0325 SA0423 SA0497 SA0498 SA0654 SA0655 SA0820 SA0905 SA0912 SA0913 SA0951 SA1056 SA1160 SA1502 SA1665 SA1850 SA1921 SA1979 SA2016 SA2093...”
- Characterization of the oxygen-responsive NreABC regulon of Staphylococcus aureus
Schlag, Journal of bacteriology 2008 - “...genes SA0187 SA0211 SA0212 SA0318 SA0580 SA0653 SA0654 SA0655 SA1014 SA1675 SA1699 SA2167 SA2209 SA2242 SA2424 SA2425 SA2426 SA2427 SA2428 fruB fruA scrA hlgB...”
- Genome-wide analysis of ruminant Staphylococcus aureus reveals diversification of the core genome
Ben, Journal of bacteriology 2008 - “...gain SA0166 Cytoplasm SA0172 Membrane SA0294 Membrane/SP SA0655 Membrane SA1156 Membrane SA0848 Cytoplasm SAR1761 Membrane/SP SA1592 Membrane SA1958 Cytoplasm...”
- Reporter metabolite analysis of transcriptional profiles of a Staphylococcus aureus strain with normal phenotype and its isogenic hemB mutant displaying the small-colony-variant phenotype
Seggewiss, Journal of bacteriology 2006 - “...SA0186 SA0639 0.00 0.00 0.01 2.19 0.38 2.04 SA0531 SA0417 SA0655 SA0374 SA2053 SA2434 0.01 0.01 0.01 0.01 0.01 0.02 2.20 2.15 2.25 0.39 2.06 2.31 a b...”
- Two novel point mutations in clinical Staphylococcus aureus reduce linezolid susceptibility and switch on the stringent response to promote persistent infection
Gao, PLoS pathogens 2010 - “...SAS1448 maltose operon transcriptional repressor 7.5 SAV0247 gatC probable PTS galactitol-specific enzyme IIC component 2.0 SAV0700 fruA PTS system, fructose-specific IIBC component 2.2 SAV1507 malA alpha-D-1,4-glucosidase 5.7 SAV1508 malR maltose operon transcriptional repressor 8.0 SAV2377 scrA PTS system, sucrose-specific IIBC component 3.0 SAV2506 gntK gluconokinase 8.5...”
- The ORFeome of Staphylococcus aureus v 1.1
Brandner, BMC genomics 2008 - “...Oligopeptide transport ATP-binding protein 530 AA SAV2029 60 kDa chaperonin 538 AA [ 41 ] SAV0700 Fructose specific permease 652 AA SAV2157 Putative uncharacterized protein 710 AA SAV1905 ATP-dependent DNA helicase pcrA 730 AA [ 42 ] Figure 3 Y2H screen for homodimers . (A) Yeast...”
EP10_002551 PTS fructose transporter subunit IIABC from Geobacillus icigianus
45% identity, 24% coverage
- The Transcriptomic Response of Cells of the Thermophilic Bacterium <i>Geobacillus icigianus</i> to Terahertz Irradiation
Peltek, International journal of molecular sciences 2024 - “...(EP10_000465) [ 24 ], the operon encoding the system of transport and utilization of fructose (EP10_002551, EP10_002552, and EP10_002553), a lactic-acid synthesis gene (EP10_003019), and genes of enzymes related to the biosynthesis of bacterial cell wall components (EP10_002881) [ 25 , 26 , 27 ] and...”
- “...the genes of the PTS system, glycolysis enzyme phosphofructokinase FruB, and a TF called FruR (EP10_002551, EP10_002552, and EP10_002553) (which is a positive regulator of genes of pentose phosphate pathway enzymes and a repressor of glycolysis enzyme genes) indicates, on the one hand, an attempt by...”
EQG65_03560, RDJ18_RS03920, SAUSA300_RS03675 fructose-specific PTS transporter subunit EIIC from Staphylococcus aureus
NWMN_0669 fructose specific permease from Staphylococcus aureus subsp. aureus str. Newman
44% identity, 20% coverage
- Multiomics analysis of Staphylococcus aureus ST239 strains resistant to virulent Herelleviridae phages
Kornienko, Scientific reports 2024 - “...glucose and fructose into the cell at both early and late stages (RDJ18_RS00835, RDJ18_RS01075, RDJ18_RS01200, RDJ18_RS03920, RDJ18_RS13495, RDJ18_RS14335) (Table S4 ). The key genes encoding enzymes involved in glycolysis exhibited upregulation: pyruvate kinase (RDJ18_RS06570), L-lactate dehydrogenase (RDJ18_RS01065), phosphoglycerate kinase (RDJ18_RS04310), triose-phosphate isomerase (RDJ18_RS04315), and 2,3-bisphosphoglycerate-independent phosphoglycerate...”
- Unveiling the fructose metabolism system in Staphylococcus aureus: insights into the regulatory role of FruR and the FruRKT operon in bacterial fitness
Ge, BMC microbiology 2024 - “...gene but few significant down-regulated genes, including the proved target genes sa03670 (SAUSA300_RS03670) and sa03675 (SAUSA300_RS03675) from the fruRKT operon (Fig. 6 ). Other than that, five genes encoding RNA polymerase sigma factor SigS, sirohydrochlorin chelatase, and function unknown protein were found down-regulated in the fruR...”
- Antibacterial Activity and Components of the Methanol-Phase Extract from Rhizomes of Pharmacophagous Plant Alpinia officinarum Hance
Fu, Molecules (Basel, Switzerland) 2022 - “...GMPE treatment. Interestingly, in the PTS, two DEGs encoding fructose transporter subunit II C ( EQG65_03560 ) and 1-phosphofructokinase ( EQG65_03555 ) were highly upregulated by 24.254- and 28.586-fold, respectively. The greatly activated monosaccharide transporter and metabolism were probably more favorable to the bacterium response to...”
- Sulfide Homeostasis and Nitroxyl Intersect via Formation of Reactive Sulfur Species in Staphylococcus aureus
Peng, mSphere 2017 - “...16.1 ATP:guanido phosphotransferase NWMN_0487 SA0483 clpC 12.7 ClpC protease, ATP-binding subunit NWMN_0587 SA0572 15.5 Hypothetical NWMN_0669 SA0655 fruA 14.2 Fructose permease (NWMN_06670669, frc operon) NWMN_0815 SA0806 17.5 8.4 NADH-dependent pyridine nucleotide disulfide oxidoreductase (short-chain dehydrogenase/reductase [SDR]) Yes (229) NWMN_0826 SA0817 13.1 NADH-dependent flavin oxidoreductase NWMN_0845 SA0835...”
CD630_22690 PTS fructose transporter subunit IIABC from Clostridioides difficile 630
CD2269 PTS system, IIbc component from Clostridium difficile 630
41% identity, 21% coverage
- The repertoire of ABC proteins in Clostridioides difficile
Pipatthana, Computational and structural biotechnology journal 2021 - “...motif and are not associated with transport activity [2] . Eleven proteins (cd630_03880, cd630_04690, cd630_08160, cd630_22690, cd630_30750, cd630_30860, cd630_30970, cd630_31160, cd630_31250, cd630_31340, and cd630_31370) annotated as phosphoryl transfer protein subunit IIABC were also omitted from further analyses. Although the phosphotransferase system (PTS) is involved in the...”
- The Clostridium difficile Protease Cwp84 Modulates both Biofilm Formation and Cell-Surface Properties
Pantaléon, PloS one 2015 - “...system, sugar-family CD630_08770 ABC-type transport system, sugar-family CD630_11840 FabF CD630_14790 FeoB1 CD630_14840 SsuA CD630_18220 Bcp CD630_22690 FruABC CD630_23880 Putative peptidoglycan-binding/hydrolysing CD630_26670 PtsG-BC CD630_27350 Cwp14 CD630_27840 Cwp6 CD630_27860 Cwp5 CD630_27980 Cwp9 CD630_28010 Conserved hypothetical protein CD630_30300 PTS system, glucose-like IIBC component CD630_31920 Cwp21 CD630_32840 Serine protease, HrtA...”
- Increased sporulation underpins adaptation of Clostridium difficile strain 630 to a biologically-relevant faecal environment, with implications for pathogenicity
Ternan, Scientific reports 2018 - “...of the sorbitol specific IIB component, srlEa , (CD0765) was increased as was expression of CD2269 encoding the fructose specific IIABC component, fruABC , as were genes encoding the IIA and IIB components of the glucose PTS transport system (CD2512, CD2510, respectively). Expression of the IIC...”
- Pleiotropic role of the RNA chaperone protein Hfq in the human pathogen Clostridium difficile
Boudry, Journal of bacteriology 2014 - “...Hfq-depleted strain while genes encoding a fructose PTS (CD2269) were upregulated. The principal source of energy in C. difficile comes from carbohydrate and...”
LMOf2365_2305 PTS system, fructose-specific, IIABC component from Listeria monocytogenes str. 4b F2365
42% identity, 20% coverage
- Role of FruR transcriptional regulator in virulence of Listeria monocytogenes and identification of its regulon
Abdelhamed, PloS one 2022 - “...Genes with significantly changed expression in fruR strain. Locus Tag Protein name Fold changes Upregulated LMOf2365_2305 PTS fructose transporter subunit IIC (FruA) 8.30 LMOf2365_2306 1-phosphofructokinase (FruB) 9.35 LMOf2365_0272 hypothetical protein 1.56 LMOf2365_0568 tagatose 1,6-diphosphate aldolase 1.77 LMOf2365_0604 GntR family transcriptional regulator 1.73 LMOf2365_0588 Magnesium transporter CorA...”
- Transcriptomics of Listeria monocytogenes Treated With Olive Leaf Extract
Liu, Frontiers in microbiology 2021 - “...addition of OLE included the phosphotransferase transport system (PTS), with the expression of 4 genes (LMOf2365_2305, LMOf2365_2664, LMOf2365_2665, and LMOf2365_2762), being differentially regulated at the 3.5-h treatment period (Log 2 FC=5.71, 1.36, 1.47, and 1.13, respectively). At 24h post-treatment, 9 PTS-related genes (LMOf2365_0030, LMOf2365_0113, LMOf2365_0572, LMOf2365_0922,...”
LMOf6854_2394 PTS system, fructose-specific, IIABC component from Listeria monocytogenes str. 1/2a F6854
42% identity, 20% coverage
SAR0753 PTS transport system, fructose-specific IIABC component from Staphylococcus aureus subsp. aureus MRSA252
44% identity, 19% coverage
- The Staphylococcus aureus response to unsaturated long chain free fatty acids: survival mechanisms and virulence implications
Kenny, PloS one 2009 - “...3.03 3.00E-04 SAR0523 SNO glutamine amidotransferase family protein 2.70 3.70E-04 SAR0752 putative phosphofructokinase 2.38 3.27E-02 SAR0753 fruA fructose-specific PTS system component 2.50 3.78E-02 SAR0766 glutamine amidotransferase class-I protein 2.04 6.16E-04 SAR1088 putative pyruvate carboxylase 2.5 8.78E-04 SAR1450 tdcB putative threonine dehydratase 2.22 5.47E-03 SAR1451 ald2 alanine...”
- “...SAR0210 putative oxidoreductase 9.09 3.75E-03 SAR0527 putative phosphotransferase 4.55 7.03E-08 SAR0752 putative phosphofructokinase 2.44 3.20E-02 SAR0753 fruA fructose-specific PTS system component 3.45 1.21E-02 SAR1274 glpF putative glycerol uptake facilitator protein 3.70 3.36E-03 SAR1275 glpK glycerol kinase 4.17 6.42E-04 SAR1276 glpD glycerol-3-phosphate dehydrogenase 7.69 4.83E-06 SAR2244 mtlA...”
Q8Y4U6 FruA protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
lmo2335 highly similar to phosphotransferase system (PTS) fructose-specific enzyme IIABC component from Listeria monocytogenes EGD-e
42% identity, 20% coverage
- Proteomic dataset of Listeria monocytogenes exposed to sublethal concentrations of free and nanoencapsulated nisin
Pinilla, Data in brief 2022 - “...Nis / LNis Annotation not available Q8Y8C6 lmo0982 lmo0982 Nis / LNis Annotation not available Q8Y4U6 lmo2335 fruA Nis / LNis FruA protein; sugar transporter, phosphoenolpyruvate-dependent phosphotransferase system Q7AP82 lmo0685 lmo0685 Nis / LNis Flagellar motor protein MotA; with MotB forms the ion channels that couple...”
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...lmo2137 PTS fructose transporter subunit IIA lmo2259 3.02 Yes/up lmo2259 PTS beta-glucoside transporter subunit IIA lmo2335 5.14 Yes/down fruA PTS fructose transporter subunit IIABC lmo2373 1.30 Yes/up lmo2373 PTS beta-glucoside transporter subunit IIB lmo2649 5.48 Yes/down ulaA PTS system ascorbate transporter subunit IIC lmo2650 5.75 Yes/down...”
- Proteomic dataset of Listeria monocytogenes exposed to sublethal concentrations of free and nanoencapsulated nisin
Pinilla, Data in brief 2022 - “.../ LNis Annotation not available Q8Y8C6 lmo0982 lmo0982 Nis / LNis Annotation not available Q8Y4U6 lmo2335 fruA Nis / LNis FruA protein; sugar transporter, phosphoenolpyruvate-dependent phosphotransferase system Q7AP82 lmo0685 lmo0685 Nis / LNis Flagellar motor protein MotA; with MotB forms the ion channels that couple flagellar...”
- Virulence gene repression promotes Listeria monocytogenes systemic infection
Pombinho, Gut microbes 2020 (secret) - Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes
Hain, BMC genomics 2012 - “...transporting PTS systems ( lmo0021-lmo0024 , lmo0781-lmo0784 , lmo1997-lmo2002 ), two fructose specific systems ( lmo2335 and lmo2733 ), two galacitol specific systems ( lmo0503 , lmo0507 , lmo0508 and lmo2665-lmo2667 ), two beta-glucoside systems (the partial system lmo0373-lmo0374 and lmo0874-lmo0876 ), and two cellobiose specific...”
- Transcriptomic and phenotypic analyses identify coregulated, overlapping regulons among PrfA, CtsR, HrcA, and the alternative sigma factors sigmaB, sigmaC, sigmaH, and sigmaL in Listeria monocytogenes
Chaturongakul, Applied and environmental microbiology 2011 - “...lmo1849, lmo1993, lmo2040, lmo2159, lmo2161, lmo2163, lmo2193, lmo2335, lmo2557, lmo2597, and lmo2785) were not included. The number of B-dependent genes...”
- Pyruvate carboxylase plays a crucial role in carbon metabolism of extra- and intracellularly replicating Listeria monocytogenes
Schär, Journal of bacteriology 2010 - “...lmo2308 lmo2309 lmo2310 lmo2311 lmo2312 lmo2315 lmo2322 lmo2335 (fruA) lmo2336 (fruB) lmo2337 lmo2343 lmo2344 lmo2345 lmo2437d lmo2522 lmo2539 (glyA) lmo2590b...”
- “...glucose control are several genes with unknown functions, lmo2335 to lmo2337 (fruRAB), encoding a PTSFru; panBCD (lmo1900 to lmo1902), involved in panthotenate...”
Ldb2040 PTS system, fructose-specific enzyme IIABC component from Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
37% identity, 23% coverage
BC3718 PTS system, fructose-specific IIABC component from Bacillus cereus ATCC 14579
43% identity, 19% coverage
T256_05730 fructose-specific PTS transporter subunit EIIC from Pediococcus pentosaceus SL4
46% identity, 19% coverage
SXYL_00278 PTS fructose transporter subunit IIABC from Staphylococcus xylosus
36% identity, 21% coverage
- Transcriptomic analysis of Staphylococcus xylosus in the presence of nitrate and nitrite in meat reveals its response to nitrosative stress
Vermassen, Frontiers in microbiology 2014 - “...Phosphotransferase system (PTS) maltose-specific enzyme IICB component 2.3 SXYL_00253 PTS system, glucose-specific IIBC component 0.5 SXYL_00278 PTS system, fructose-specific IIABC components 3.4 SXYL_00626 PTS system arbutin-like IIBC component 1.5 SXYL_01351 malA Alpha-D-1,4-glucosidase 1.9 SXYL_01960 pgi Glucose-6-phosphate isomerase 2.2 SXYL_00426 fbp Fructose-1,6-bisphosphatase 1.8 SXYL_00039 iolJ Fructose-bisphosphate aldolase,...”
- “...a crucial role in redox metabolism (Kleijn et al., 2006 ). Four genes (SXYL_02255, SXYL_00253, SXYL_00278, SXYL_00626) encoding phosphotransferase systems (PTS) that catalyze the concomitant uptake and phosphorylation of carbohydrates were modulated (Table 1 ). The two genes paralogs (SXYL_02255, SXYL_00626) coding for a PTS for...”
CTK_C06320 fructose-specific PTS transporter subunit EIIC from Clostridium tyrobutyricum
39% identity, 22% coverage
- Deciphering Clostridium tyrobutyricum Metabolism Based on the Whole-Genome Sequence and Proteome Analyses
Lee, mBio 2016 - “...ATPase domain 3 0.99 4.67 2 CTK_C29270 Transcription elongation factor GreA 2 0.65 3.90 3 CTK_C06320 Fructose-specific PTS (phosphotransferase system) system enzyme IIABC component 4 3.16 3.70 4 CTK_C06300 Predicted transcriptional regulator, DeoR family 2 3.23 3.63 5 CTK_C06310 1-Phosphofructokinase 5 3.27 3.33 6 CTK_C21760 Anaerobic-type...”
- “...major route for glucose transport in C.tyrobutyricum . In addition to glucose transporters, fructose-specific PTS (CTK_C06320; enzyme IIABC [EIIABC]) was abundantly expressed at the early exponential phase (C2), but its expression level decreased dramatically at the later phases ( Table3 ). This transient expression of the...”
LBA1777 PTS system, fructose-specific enzyme II from Lactobacillus acidophilus NCFM
39% identity, 22% coverage
CPE0584 PTS system from Clostridium perfringens str. 13
36% identity, 24% coverage
- RNA-seq analysis of virR and revR mutants of Clostridium perfringens
Low, BMC genomics 2016 - “...protein 1.53 1.93 Others b cpe2562 cspB protease 3.87 4.49 cpe0855 rubY rubrerythrin 1.42 1.66 cpe0584 fruA PTS fructose transporter 1.48 1.83 cpe0638 pstC phosphate ABC transporter permease 1.27 6.50 cpe0094 nirC nitrite transporter 1.75 5.13 a As defined by FDR <0.01; log 2 fold change>1...”
SE2228 PTS system fructose-specific II component from Staphylococcus epidermidis ATCC 12228
SERP2260 PTS system, fructose-specific IIABC components from Staphylococcus epidermidis RP62A
SERP_RS11135 fructose-specific PTS transporter subunit EIIC from Staphylococcus epidermidis RP62A
34% identity, 22% coverage
- Identification of antigenic components of Staphylococcus epidermidis expressed during human infection
Pourmand, Infection and immunity 2006 - “...Conserved hypothetical protein 26 2283238-2285302 SE2228 (105-646) SE2229 (1-141) Phosphotransferase system fructose-specific II component Mannose-6-phosphate...”
- Skin-to-blood pH shift triggers metabolome and proteome global remodelling in Staphylococcus epidermidis
Gonçalves, Frontiers in microbiology 2022 - “...Carbamate kinase SERP2352 arcC Delta-1-pyrroline-5-carboxylate dehydrogenase SERP2128 rocA Phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) Fructose-specific IIABC components SERP2260 Sucrose-specific IIBC components SERP1900/68 Glucose-specific EIICBA component SERP2114 ptsG 6-Phospho-beta-galactosidase SERP1789 lacG Tagatose 1,6-diphosphate aldolase SERP1792 lacD Membrane Transporters: Hemin, Iron and Mn Multidrug resistance efflux pump SERP1767 SepA Flotillin-like...”
- “...their translocation across the cell membrane. Four proteins from this system (PtsG, SERP1900, SERP1968 and SERP2260) specific for glucose, sucrose and fructose transport, and enzymes generating phosphoenolpyruvate from oxaloacetate (PckA) and pyruvate (PpdK and its regulator SERP2169) support the fuelling of glycolysis in the N77 condition....”
- The Vancomycin Resistance-Associated Regulatory System VraSR Modulates Biofilm Formation of Staphylococcus epidermidis in an ica-Dependent Manner
Wu, mSphere 2021 - “...genes such as serp2114 (phosphotransferase [PTS] glucose EIICBA component), serp2343 (PTS mannose transporter subunit IIA), serp2260 (fructose-specific IIABC components), and lacF (PTS lactose-specific phosphotransferase IIA component) ( Table2 ). TABLE2 Transcription levels of genes involved in antibiotic tolerance and biofilm formation of S. epidermidis vraSR Gene...”
- AI-2-dependent gene regulation in Staphylococcus epidermidis
Li, BMC microbiology 2008 - “...operon transcriptional repressor 0.37 6.03 SERP2100 Ribokinase 0.49 2.71 SERP2101 Ribose transport protein 0.42 3.14 SERP2260 PTS system, fructose-specific IIABC components 0.29 8.11 SERP2261 Mannose-6-phosphate isomerase 0.34 5.9 SERP2312 Malate:quinone oxidoreductase 2.64 0.33 Nucleotide metabolism SERP0067 Xanthine phosphoribosyltransferase 3.17 0.4 SERP0068 Xanthine permease 3.35 0.39 SERP0652...”
- The Vancomycin Resistance-Associated Regulatory System VraSR Modulates Biofilm Formation of Staphylococcus epidermidis in an ica-Dependent Manner
Wu, mSphere 2021 - “...mannose transporter subunit IIA 0.41 ND SERP_RS11515 NC_002976.3 (23899822390557) Dihydroxyacetone kinase subunit L 0.46 ND SERP_RS11135 NC_002976.3 (22894202291360) PTS system, fructose-specific IIABC components 0.27 ND SERP_RS09555 NC_002976.3 (19283961929985) PTS alpha-glucoside transporter subunit IIBC 0.17 ND lacF NC_002976.3 (18381491838463) PTS system lactose-specific phosphotransferase IIA component 0.40 ND...”
Cbei_1844 PTS system, fructose subfamily, IIC subunit from Clostridium beijerincki NCIMB 8052
42% identity, 20% coverage
X276_17630 fructose-specific PTS transporter subunit EIIC from Clostridium beijerinckii NRRL B-598
42% identity, 20% coverage
PTN3B_BACSU / O31645 PTS system mannose-specific EIIBCA component; EIIBCA-Man; EII-Man; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see 2 papers)
O31645 protein-Npi-phosphohistidine-D-mannose phosphotransferase (EC 2.7.1.191) from Bacillus subtilis (see paper)
TC 4.A.2.1.6 / O31645 The mannose porter (ManP) (37% identical to 4.A.2.1.1). It is encoded in an operon with 3 genes: from Bacillus subtilis (see 5 papers)
NP_389083 phosphotransferase system (PTS) mannose-specific enzyme IIBCA component from Bacillus subtilis subsp. subtilis str. 168
BSU12010 phosphotransferase system (PTS) mannose-specific enzyme IIBCA component from Bacillus subtilis subsp. subtilis str. 168
44% identity, 16% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannose transport
catalytic activity: D-mannose(out) + N(pros)-phospho-L-histidyl-[protein] = D- mannose 6-phosphate(in) + L-histidyl-[protein] (RHEA:49232)
disruption phenotype: Cells lacking this gene are unable to grow with mannose as the sole carbon source. Deletion of manP results in constitutive expression from both the manP and manR promoters, indicating that the phosphotransferase system (PTS) component EII-Man has a negative effect on regulation of the mannose operon and manR. - substrates: Mannose
tcdb comment: manP-manA-yjdD, where manP codes for the IIBCA mannose transporter, manA codes for a mannose-6-P isomerase and YjdD codes for a 5-formyltetrahydrofolate cyclo-ligase, characterized in B. anthrasis. Expression of the operon is regulated by ManP and ManR, an activator, in response to external mannose (Sun and Altenbuchner 2010) - Characterization of a mannose utilization system in Bacillus subtilis.
Sun, Journal of bacteriology 2010 - GeneRIF: The phosphoenolpyruvate (PEP)-dependent phosphorylation by the histidine protein HPr via His15 plays an essential role in transcriptional activation of P(manP) and P(manR).
- Comparison of Bacillus subtilis transcriptome profiles from two separate missions to the International Space Station
Morrison, NPJ microgravity 2019 - “...2.52 2.61 1.17 1.10 Oligopeptide ABC transporter, inactive pseudogene in strain 168 CodY, ScoC, TnrA BSU12010 manP 2.38 2.67 1.38 1.47 PTS system-mannose-specific transporter subunit EIIBCA ManR, SigA BSU15960 sivC 2.89 3.06 2.67 2.71 Inhibitor of entry into sporulation via KinB or KinC AbrB, SigD BSU18000...”
NCgl1861 fructose-specific PTS transporter subunit EIIC from Corynebacterium glutamicum ATCC 13032
cg2120 sugar specific PTS system, fructose/mannitol-specific transport protein from Corynebacterium glutamicum ATCC 13032
43% identity, 18% coverage
- The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum
Gaigalat, BMC molecular biology 2007 - “...- 264 28.0 Putative transcriptional regulator protein, DeoR-family cg2119 NCgl1860 fruK 330 34.0 1-phosphofructokinase cg2120 NCgl1861 ptsF 688 70.5 Fructose-specific PTS component, enzyme IIABC cg2121 NCgl1862 ptsH 89 9.1 Phosphocarrier protein HPr, PTS component non clustered cg1537 NCgl1305 ptsG 683 72.6 Glucose-specific PTS component, enzyme IIBCA...”
- “...Hypothetical protein cg2925 NCgl2553 ptsS 3.39 11.87 Phosphotransferase system (PTS), sucrose-specific enzyme IIBCA component cg2120 NCgl1861 ptsF 3.27 12.04 Phosphotransferase system (PTS), fructose-specific enzyme IIABC component cg3107 NCgl2709 adhA 3.23 13.00 Alcohol dehydrogenase cg2118 NCgl1859 - 3.00 12.00 Transcriptional regulator protein, DeoR-family cg1537 NCgl1305 ptsG 2.70...”
- The DeoR-type regulator SugR represses expression of ptsG in Corynebacterium glutamicum
Engels, Journal of bacteriology 2007 - “...the gene of the fructose-specific enzyme II permease ptsF (NCgl1861) and might therefore be involved in the regulation of sugar uptake via the PTS and/or the...”
- Two-component systems of Corynebacterium glutamicum: deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response
Kocan, Journal of bacteriology 2006 - “...cg2846 NCgl1444 NCgl1482 NCgl1521 NCgl1646 NCgl1859 NCgl1861 NCgl1915 NCgl1917 NCgl1959 NCgl2001 NCgl2127 NCgl2128 NCgl2393 NCgl2409 NCgl2434 NCgl2439 NCgl2483...”
- Substrate-dependent cluster density dynamics of Corynebacterium glutamicum phosphotransferase system permeases
Martins, Molecular microbiology 2019 - “...the glucosespecific membrane integral EIIBCA transporter, while ptsS (cg2925) encodes the sucrosespecific EIIBCA, and ptsF (cg2120) the fructosespecific EIIABC (Fig. 1 ). A fourth putative PTS belongs to the Lascorbatetype family and is encoded by rmpC (cg3365) (not depicted in the graphic), but no function has...”
- Elastase digests: new ammunition for shotgun membrane proteomics
Rietschel, Molecular & cellular proteomics : MCP 2009 - “...6 74 71 65 62 72 46 22 10 Cg0446 Cg2705 Cg3009 Cg2181 7 47 3 Cg2120 8 9 10 39 8 44 47 46 22 Cg2409 Cg0587 Cg2911 11 12 13 14 15 41 39 30 29 28 22 2 17 9 26...”
- The global repressor SugR controls expression of genes of glycolysis and of the L-lactate dehydrogenase LdhA in Corynebacterium glutamicum
Engels, Journal of bacteriology 2008 - “...gene product, and/or deduced function cg2925 cg2119 cg2120 cg2118 cg2117 cg2121 cg3219 ptsS, sucrose-specific enzyme II of the PTS pfkB, 1-phosphofructokinase...”
- The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Corynebacterium glutamicum
Gaigalat, BMC molecular biology 2007 - “...NCgl1859 - 264 28.0 Putative transcriptional regulator protein, DeoR-family cg2119 NCgl1860 fruK 330 34.0 1-phosphofructokinase cg2120 NCgl1861 ptsF 688 70.5 Fructose-specific PTS component, enzyme IIABC cg2121 NCgl1862 ptsH 89 9.1 Phosphocarrier protein HPr, PTS component non clustered cg1537 NCgl1305 ptsG 683 72.6 Glucose-specific PTS component, enzyme...”
- “...to this, all genes of the fructose-PTS gene cluster from cg2117 ( ptsI ) to cg2120 ( ptsH ) showed significantly higher mRNA levels during growth on glucose and fructose. The levels of the genes ptsI and ptsH did not differ between cells grown on glucose...”
- Two-component systems of Corynebacterium glutamicum: deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response
Kocan, Journal of bacteriology 2006 - “...NCgl1209 NCgl1210 NCgl1232 cg1695 cg1737 cg1785 cg1930 cg2118 cg2120 cg2181 cg2183 cg2234 cg2283 cg2422 cg2423 cg2726 cg2743 cg2777 cg2782 cg2843 cg2844 cg2845...”
- Global gene expression during stringent response in Corynebacterium glutamicum in presence and absence of the rel gene encoding (p)ppGpp synthase
Brockmann-Gretza, BMC genomics 2006 - “...1.79 sigB RNA polymerase sigma factor K cg2114 1.59 lexA LexA repressor transcriptional regulator KT cg2120 1.53 ptsF PTS system, fructose/mannitol-specific enzyme II G cg2291 1.53 pyk pyruvate kinase G cg2398 1.55 plsC 1-acyl-SN-glycerol-3-phosphate acetyltransferase I cg2403 2.61 qcrB cytochrome B C cg2405 1.69 qcrC cytochrome...”
tagM / A0A1Y0YTF1 tagatose-specific PTS enzyme IIA component from Bacillus licheniformis (see 2 papers)
TC 4.A.2.1.23 / T5HNP5 PTS fructose transporter subunit IIA, component of The tagatose-1-P-forming tagatose phosphorylating Enzyme IIA/IIBC, TagM/L (Van der Heiden et al. 2015). The product is phosphorylated by tagatose-1-P kinase (TagK), and then cleaved by tagatose-1,6-bisphosphate aldolase (GatY) from Bacillus licheniformis CG-B52
38% identity, 84% coverage
HGB56_07735 PTS sugar transporter subunit IIA from Lactiplantibacillus plantarum
39% identity, 94% coverage
- The Carbohydrate Metabolism of Lactiplantibacillus plantarum
Cui, International journal of molecular sciences 2021 - “...systems (PTSs) responsible for fructose transport, which were encoded by fruA (HGB56_02940), fruA1-fruA2-fruB (HGB56_07725, HGB56_07730, HGB56_07735), pts9ABCD (HGB56_11950, HGB56_11955, HGB56_11960) and pts10AB (HGB56_11975, HGB56_11980) genes, respectively ( Supplementary Table S3-4 ). Fructose enters cells in the form of 6-phosphate-fructose through these PTSs, then 6-phosphate-fructose is transformed...”
lmo0426 similar to PTS fructose-specific enzyme IIA component from Listeria monocytogenes EGD-e
35% identity, 97% coverage
- Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...lmo0399 PTS fructose transporter subunit IIB lmo0400 9.39 Yes/down lmo0400 PTS fructose transporter subunit IIC lmo0426 1.75 Yes/up 2.63 Yes/down lmo0426 PTS fructose transporter subunit IIA lmo0427 1.65 Yes/up lmo0427 PTS fructose transporter subunit IIB lmo0428 1.95 Yes/up 2.82 Yes/down lmo0428 PTS fructose transporter subunit IIC...”
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - New Insights into the Lactic Acid Resistance Determinants of Listeria monocytogenes Based on Transposon Sequencing and Transcriptome Sequencing Analyses
Liu, Microbiology spectrum 2023 - “...fructose transporter subunit IIB 0.1191 4.36E-10 lmo0400 lmo0400 PTS fructose transporter subunit IIC 0.0261 8.89E-14 lmo0426 lmo0426 PTS fructose transporter subunit IIA 0.2280 8.76E-07 lmo0427 lmo0427 PTS fructose transporter subunit IIB 0.2581 1.04E-03 lmo0428 lmo0428 PTS fructose transporter subunit IIC 0.2212 5.17E-26 lmo0503 lmo0503 PTS fructose...”
- YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes
Cheng, Gut microbes 2021 - “...Yes lmo2797 PTS mannitol-specific enzyme IIA 6.64 Yes lmo2099 PTS mannitol/fructose-specific enzyme IIA 5.23 Yes lmo0426 PTS fructose-specific enzyme IIA 5.07 Yes lmo2667 PTS galactitol-specific enzyme IIA 4.87 Yes lmo2098 PTS galactitol-specific enzyme IIA 4.85 Yes lmo0425 PTS mannitol/fructose-specific enzyme IIA 4.11 Yes lmo2668 PTS mannitol/fructose-specific...”
- Colonisation dynamics of Listeria monocytogenes strains isolated from food production environments
Gray, Scientific reports 2021 - “...system cellobiose-specific IIA component 2.7.1.205 lmo0427 fig|1639.4014.peg.2115 4.77 G PTS system, Lactose/Cellobiose specific IIB subunit lmo0426 fig|1639.4014.peg.2116 4.02 G PEP-dependent sugar PTS, EIIA 2 lmo0024 fig|1639.4014.peg.434 4.99 G PTS system mannose/fructose/sorbose family IID component lmo0034 fig|1639.4014.peg.444 5.61 G PTS system cellobiose-specific IIC component lmo0374 fig|1639.4014.peg.610 3.66...”
- Transcriptomic and phenotypic analyses identify coregulated, overlapping regulons among PrfA, CtsR, HrcA, and the alternative sigma factors sigmaB, sigmaC, sigmaH, and sigmaL in Listeria monocytogenes
Chaturongakul, Applied and environmental microbiology 2011 - “...Foster City, CA), for the genes lmo0402, lmo0422, lmo0426, lmo1849, and lmo2648. Further qRT-PCR assays were performed to confirm key cross-regulation between...”
- “...One gene in each predicted C-dependent operon (lmo0402, lmo0426, lmo1849, and lmo2648), as well as a single gene with an apparent upstream C-dependent promoter...”
- The transcriptional response of Listeria monocytogenes during adaptation to growth on lactate and diacetate includes synergistic changes that increase fermentative acetoin production
Stasiewicz, Applied and environmental microbiology 2011 - “...ABC transporter genes (fructose transporter genes lmo0357, lmo0426, and lmo2137 fruA), prophage genes (lmo2283, lmo2284, lmo2286, and lmo2293), zinccontaining...”
- Glycerol metabolism and PrfA activity in Listeria monocytogenes
Joseph, Journal of bacteriology 2008 - “...lmo2002), cellobiose (lmo2683 to lmo2685 and lmo2708), fructose (lmo0426 to lmo0428), and galactitol (lmo2665 to lmo2667); the gene for a conjugated bile acid...”
lmo0357 similar to PTS system, enzyme IIA component from Listeria monocytogenes EGD-e
34% identity, 95% coverage
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...4.42 Yes/down lmo0299 PTS beta-glucoside transporter subunit IIB lmo0301 lmo0301 PTS beta-glucoside transporter subunit IIA lmo0357 lmo0357 PTS sugar transporter subunit IIA lmo0358 1.89 Yes/down lmo0358 PTS fructose transporter subunit IIBC lmo0373 2.47 Yes/down lmo0373 PTS beta-glucoside transporter subunit IIC lmo0374 2.43 Yes/down lmo0374 PTS beta-glucoside...”
- New Insights into the Lactic Acid Resistance Determinants of Listeria monocytogenes Based on Transposon Sequencing and Transcriptome Sequencing Analyses
Liu, Microbiology spectrum 2023 - “...beta-glucoside transporter subunit IIB 0.0042 7.70E-10 lmo0301 lmo0301 PTS beta-glucoside transporter subunit IIA 0.0549 2.20E-06 lmo0357 lmo0357 PTS sugar transporter subunit IIA 0.3374 1.68E-03 lmo0358 lmo0358 PTS fructose transporter subunit IIBC 0.2495 2.06E-04 lmo0374 lmo0374 PTS beta-glucoside transporter subunit IIB 0.2696 4.37E-03 lmo0399 lmo0399 PTS fructose...”
- The transcriptional response of Listeria monocytogenes during adaptation to growth on lactate and diacetate includes synergistic changes that increase fermentative acetoin production
Stasiewicz, Applied and environmental microbiology 2011 - “...sugar ABC transporter genes (fructose transporter genes lmo0357, lmo0426, and lmo2137 fruA), prophage genes (lmo2283, lmo2284, lmo2286, and lmo2293),...”
- Probing the pan-genome of Listeria monocytogenes: new insights into intraspecific niche expansion and genomic diversification
Deng, BMC genomics 2010 - “...LIII Gene 1 Annotation LIII 2 L. innocua 3 Operon 4 Carbohydrate transport and metabolism lmo0357 Similar to PTS system, enzyme IIA component IIIA + 059 lmo0358 Similar to PTS system, fructose-specific enzyme IIBC component IIIA + 059 lmo0359 Similar to D-fructose-1,6-biphosphate aldolase" IIIA + -...”
CD3015 PTS system, IIa component from Clostridium difficile 630
35% identity, 95% coverage
XNRR2_0028 fructose-specific PTS transporter subunit EIIC from Streptomyces albidoflavus
49% identity, 17% coverage
- Superior production of heavy pamamycin derivatives using a bkdR deletion mutant of Streptomyces albus J1074/R2
Gläser, Microbial cell factories 2021 - “...l -valine, cells exhibited a down-regulation of sugar transport genes encoding: the fructose-specific IIA/B/C component (XNRR2_0028) and the phosphor carrier protein HPr (XNRR2_0029) of the phosphotransferase system (PTS), and a sugar transporter with presumed function as mannitol permease (XNRR2_0970) (Additional file 1 : Table S3). Additionally,...”
- “...Notably, the repressing effects of l -valine on sugar uptake was diminished. The PTS genes (XNRR2_0028, XNRR2_0029), and the mannitol permease encoding gene (XNRR2_0970) were not downregulated in bkdR , different to the wild type. In addition, other PTS components (XNRR2_5450, XNRR2_5451) were slightly upregulated (Additional...”
CAETHG_0142, CLJU_RS10110, CLJU_c20590 PTS fructose transporter subunit IIABC from Clostridium ljungdahlii DSM 13528
37% identity, 21% coverage
- Interactive Regulation of Formate Dehydrogenase during CO2 Fixation in Gas-Fermenting Bacteria
Zhang, mBio 2020 - “...mutations at acetylated lysine sites on CcpA DNA-binding activity. Binding to the promoter of the CLJU_c20590 gene was determined by EMSA. Download FIGS5, TIF file, 1.6 MB . Copyright 2020 Zhang et al. 2020 Zhang et al. This content is distributed under the terms of the...”
- “...485-bp DNA fragment covering the promoter region (which contains a CcpA-binding cre site) of the CLJU_c20590 gene was obtained by PCR amplification and then used as the DNA probe in EMSA ( Fig.3C ). As shown in Fig.3D , compared to the original CcpA, CcpA Dat...”
- Transcriptomic profiles of Clostridium ljungdahlii during lithotrophic growth with syngas or H2 and CO2 compared to organotrophic growth with fructose
Aklujkar, Scientific reports 2017 - “...to organotrophic growth on fructose encode the fructose transport phosphotransferase system-specific membrane protein ( fruA CLJU_RS10110), the enzyme fructose-1-phosphate 6-kinase ( fruK CLJU_RS10115) that is only required for utilization of exogenous fructose (Fig. 5 ), and the fructose-1-phosphate-responsive transcriptional regulator ( fruR CLJU_RS10120, Supplementary Table S2...”
- Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia
Brown, Biotechnology for biofuels 2014 - “...2062, 2479, T 7 Fumarate reductase CAETHG_0344, 1032, 2961. Glycolysis/Gluconeogenesis. PTS Fructose phosphotransferase system (PTS) CAETHG_0142, 0676-73, G 1 Fructokinase (Fk) /Fructose-6-phosphate isomerase CAETHG_0166, 0156, G 2 1-phosphofructokinase (Pfk1) CAETHG_0143, G 3 6-phosphofructokinase (Pfk6) CAETHG_648, 2439, G 4 Fructose bisphosphate aldolase (Aldo) CAETHG_2382, G 5 Triose-phosphate...”
- Clostridium ljungdahlii represents a microbial production platform based on syngas
Köpke, Proceedings of the National Academy of Sciences of the United States of America 2010 - “...mannitol-specific phosphotransferase (PTS) systems (encoded by CLJU_c20590 and CLJU_c26050-70) and can be further converted by 1-phosphofructokinase (encoded by...”
D9QAX8 PTS transporter subunit EIIA from Corynebacterium pseudotuberculosis (strain C231)
39% identity, 20% coverage
- Changes in protein abundance are observed in bacterial isolates from a natural host
Rees, Frontiers in cellular and infection microbiology 2015 - “...transport and metabolism D9QAU4 rnd CpC231_1194 Ribonuclease D 1.427 * Translation ribosomal structure and biogenesis D9QAX8 ptsF CpC231_1229 PTS system fructose-specific EIIABC component 0.108 2.542 * 2.665 * Carbohydrate transport and metabolism D9QBD3 ftsQ CpC231_1388 Cell division protein FtsQ 1.030 * 0.029 Cell wall/membrane/envelope biogenesis D9QBF3...”
TPASS_20085 PTS sugar transporter subunit IIA from Treponema pallidum subsp. pallidum SS14
TP0085 PTS system, nitrogen regulatory IIA component (ptsN-1) from Treponema pallidum subsp. pallidum str. Nichols
36% identity, 95% coverage
LMOf2365_0442 PTS system, fructose-specific, IIA component from Listeria monocytogenes str. 4b F2365
34% identity, 97% coverage
- Contributions of a LysR Transcriptional Regulator to Listeria monocytogenes Virulence and Identification of Its Regulons
Abdelhamed, Journal of bacteriology 2020 (secret) - LMOf2365_0442 Encoding for a Fructose Specific PTS Permease IIA May Be Required for Virulence in L. monocytogenes Strain F2365
Liu, Frontiers in microbiology 2017 - “...Microbiol Front. Microbiol. Frontiers in Microbiology 1664-302X Frontiers Media S.A. 5581801 10.3389/fmicb.2017.01611 Microbiology Original Research LMOf2365_0442 Encoding for a Fructose Specific PTS Permease IIA May Be Required for Virulence in L. monocytogenes Strain F2365 Liu Yanhong 1 * Yoo Brian B. 2 Hwang Cheng-An 3 Suo...”
- “...causes listeriosis, which is a major public health concern due to the high fatality rate. LMOf2365_0442, 0443 , and 0444 encode for fructose-specific EIIABC components of phosphotransferase transport system (PTS) permease that is responsible for sugar transport. In previous studies, in-frame deletion mutants of a putative...”
- Construction of Listeria monocytogenes mutants with in-frame deletions in the phosphotransferase transport system (PTS) and analysis of their growth under stress conditions
Liu, Journal of food science 2013 (PubMed)- “...survival mechanisms under these stress conditions. LMOf2365_0442, 0443, and 0444 encode for phosphotransferase transport system (PTS) permease...”
- “...genes, deletion mutants of the PTS genes (LMOf2365_0442, LMOf2365_0443, and LMOf2365_0444) and the downstream gene LMOf2365_0445 were created in L....”
EHLA_0331 PTS fructose transporter subunit IIABC from Anaerobutyricum hallii
41% identity, 20% coverage
Halsa_2283 PTS sugar transporter subunit IIA from Halanaerobium hydrogeniformans
30% identity, 96% coverage
- Going from microbial ecology to genome data and back: studies on a haloalkaliphilic bacterium isolated from Soap Lake, Washington State
Mormile, Frontiers in microbiology 2014 - “...FruA ) are also present. Halanaerobium hydrogeniformans has two putative nitrogen-related PTS genes, Halsa_0019 and Halsa_2283. Nitrogen-related PTS genes are found in Gram-negative bacteria, can regulate carbon and nitrogen metabolism, are required for virulence by some bacteria, and can play a role in potassium homeostasis (...”
- “...with a fructose-specific PTS from Halanaerobium saccharolyticum and Halanaerobium praevalens , respectively. The role for Halsa_2283 isnt as apparent as for Halsa_0019. The gene in the same operon, Halsa_2284, was not identified. In addition, when a BLAST search was performed on the amino acid sequence encoded...”
OKIT_0249 PTS sugar transporter subunit IIA from Oenococcus kitaharae DSM 17330
38% identity, 82% coverage
TC 4.A.2.1.20 / Q3K0G6 Chromosomal fructose Enzyme IIABC (Fru1) of 654 aas; in an operon with fructose-1-P kinase from Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
SAK_1377 PTS system, fructose-specific IIABC component from Streptococcus agalactiae A909
36% identity, 20% coverage
- substrates: fructose
- The Role of Regulator Catabolite Control Protein A (CcpA) in Streptococcus agalactiae Physiology and Stress Response
Roux, Microbiology spectrum 2022 - “..., galactitol PTS, IIA, IIC, and IIB subunits; SAK_0915 , PTS system, -glucoside-specific IIABC component; SAK_1377 , PTS system, fructose-specific IIABC component; SAK_1702 , PTS system, sucrose-specific IIABC component; SAK_1893-1895 , PTS system, glucose-specific IIABC component; SAK_1908-1911 , mannose/fructose/sorbose PTS, IID, IIC, IIB, and IIA subunits,...”
- A Vaginal Tract Signal Detected by the Group B Streptococcus SaeRS System Elicits Transcriptomic Changes and Enhances Murine Colonization
Cook, Infection and immunity 2018 - “...sak_0524 sak_0525 sak_0526 sak_0528 sak_0529 sak_0530 sak_1377 sak_1702 sak_1759 sak_1825 sak_1833 sak_1834 sak_1835 sak_1893 sak_1894 sak_1895 sak_1908...”
- “...sak_0594 sak_0981 sak_0982 sak_0980 sak_0983 sak_0985 sak_1377 sak_1759 sak_1911 sak_1910 sak_1909 sak_1908 sak_1751 sak_0666 Two-component systems sak_0467...”
LCA_RS05240 fructose-specific PTS transporter subunit EIIC from Latilactobacillus sakei subsp. sakei 23K
37% identity, 20% coverage
- Transcriptome Analysis Reveals the Role of Sucrose in the Production of Latilactobacillus sakei L3 Exopolysaccharide
Wang, International journal of molecular sciences 2024 - “...5 and Table 3 , the PTS-S (LCA_RS08985) and PTS fructose transporter subunit IIC (PTS-F, LCA_RS05240), assigned to the phosphotransferase system (PTS) relating to the transport and metabolism of sugars from the extracellular to the intracellular sides of cells [ 24 ], were significantly up-regulated, indicating...”
- “...Figure 12 Validation of selected DEGs from sucrose metabolism using RT-qPCR. ( a ) PTS-F (LCA_RS05240); ( b ) PTS-S (LCA_RS08985); ( c ) sucrose-6-phosphate hydrolase (LCA_RS08990). ijms-25-07185-t001_Table 1 Table 1 Clean read quality metrics. Sample Total Raw Reads (Mb) Total Clean Reads (Mb) Total Clean...”
stu0401 fructose PTS system component IIABC, truncated from Streptococcus thermophilus LMG 18311
39% identity, 50% coverage
SSU0768 fructose-specific phosphotransferase system (PTS), IIABC component from Streptococcus suis P1/7
B9H01_RS04040 fructose-specific PTS transporter subunit EIIC from Streptococcus suis
39% identity, 20% coverage
- FlpS, the FNR-Like Protein of Streptococcus suis Is an Essential, Oxygen-Sensing Activator of the Arginine Deiminase System
Willenborg, Pathogens (Basel, Switzerland) 2016 - “...maltose degradation were upregulated. In the exp growth phase the predicted PTS for fructose ( SSU0768 ), trehalose ( SSU0217 ) and glucose/mannose ( SSU1583 ), and in the stat growth phase the PTS for predicted transport of mannose ( SSU0199-201 ), galactose ( SSU0403-0406 ),...”
- Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig
Zhang, BMC veterinary research 2023 - “...change Fructose and mannose metabolism B9H01_RS08575 B9H01_RS08575 K02795 manY 0.79238 B9H01_RS04035 pfkB K00882 fruK 0.877493 B9H01_RS04040 B9H01_RS04040 K02770 fruA 0.819678 B9H01_RS08580 B9H01_RS08580 K02796 manZ 0.832418 Glycerolipid metabolism B9H01_RS07520 B9H01_RS07520 K07407 galA 0.666382 B9H01_RS03575 glpK K00864 glpK 0.85005 ABC transporters B9H01_RS09200 B9H01_RS09200 K17319 lplB 0.682764 B9H01_RS09195 B9H01_RS09195...”
- “...B9H01_RS01065 B9H01_RS01065 K03475 ulaA 0.720561893 B9H01_RS04035 pfkB K00882 fruK 0.87749257 B9H01_RS01165 B9H01_RS01165 K02761 celB 0.749690724 B9H01_RS04040 B9H01_RS04040 K02770 fruA 0.819677837 B9H01_RS08580 B9H01_RS08580 K02796 manZ 0.832418165 B9H01_RS09285 B9H01_RS09285 K02810 scrA 0.800577967 The GO analysis of differentially expressed genes was used to annotate the 117 up-regulated genes. Translation,...”
SACOL2663 PTS system, fructose-specific IIABC components from Staphylococcus aureus subsp. aureus COL
NWMN_2540 PTS system, fructose-specific IIABC component from Staphylococcus aureus subsp. aureus str. Newman
38% identity, 16% coverage
- Virulence and Metabolism
Richardson, Microbiology spectrum 2019 (secret) - Changes in the Staphylococcus aureus transcriptome during early adaptation to the lung
Chaffin, PloS one 2012 - “...in vitro conditions. Trehalose, glycerol-3-phosphate, fructose and ascorbate-specific transport systems ( treP , glpT , SACOL2663 and ulaA ) had up to 47.0 fold lower transcription in vivo. Genes with high expression in in vitro but lowest expression in vivo also included SACOL-2521, a protein, similar...”
- “...systems. These included the glucose-specific PTS IIABC transporter component ptsG , fructose/(mannose)-specific PTS IIABC component SACOL2663, maltose/maltodextrin ABC permease malK and glycerol-3-phosphate transporter gene, glpT . Also upregulated were the phosphate transporter ptsS , sodium:solute symporter family gene SACOL0311 and putative transport gene SACOL2521. Like the...”
- A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome
Becher, PloS one 2009 - “...(Gpm), SACOL1320 (GlpK), SACOL1319 (GlpF), SACOL0200, SACOL0407 (GlpT)], or consumption of alternative sugars [SACOL1393 (GlcT), SACOL2663, SACOL0516, SACOL0178, SACOL0224, SACOL2316]. Of the proteins down-regulated in stationary phase numerous could be assigned to the category of iron uptake facilitation [SACOL2010, SACOL2277, SACOL2167, SACOL0799, SACOL0099 (SirA), SACOL0704] or...”
- A defect in menadione biosynthesis induces global changes in gene expression in Staphylococcus aureus
Kohler, Journal of bacteriology 2008 - “...of trehalose (SACOL0516), mannitol (SACOL2146), and fructose (SACOL2663) and also of glucose (ptsI, SACOL0175, SACOL2552) (see Table S2 in the supplemental...”
- Inactivation of the Ecs ABC transporter of Staphylococcus aureus attenuates virulence by altering composition and function of bacterial wall
Jonsson, PloS one 2010 - “...permease 4.5 NWMN_2458 cation transporter E1-E2 family ATPase 1.8 2.6 NWMN_2521 permease domain-containing protein 2.0 NWMN_2540 PTS system fructose-specific IIABC component 0.5 NWMN_2592 sodium sulfate symporter 1.8 NWMN_2593 RarD protein 3.5 opp1F peptide ABC transporter ATP-binding protein 2.2 opuCD amino acid ABC transporter permease protein 2.0...”
SA2434 hypothetical protein from Staphylococcus aureus subsp. aureus N315
38% identity, 16% coverage
- Exposure of clinical MRSA heterogeneous strains to β-lactams redirects metabolism to optimize energy production through the TCA cycle
Keaton, PloS one 2013 - “...system (PTS) SA2320 glcA 4.6 PTS system glucose-specific EIICBA component Phosphotransferase transport system (PTS, glucose-specific). SA2434 4.2 PTS system EIIBC component Putative phosphotransferase transport system (PTS), EIIBC component. SA0186 ptsG 4 PTS system glucoside-specific IICBA component Phosphotransferase transport system (PTS), glucoside specific. SA0325 glpT 2.7 glycerol-3-phosphate...”
- “...mannose specific PTS (SA2527), glucose specific PTS ( glcA , SA0183), putative fructose specific PTS (SA2434), glucoside specific PTS ( ptsG , SA2326) and glucose specific PTS (SA1566). A group of hypothetical proteins predicted to be related to carbohydrate metabolism were downregulated during HeR/HoR selection, including...”
- Global transcriptome analysis of Staphylococcus aureus biofilms in response to innate immune cells
Scherr, Infection and immunity 2013 - “...SA2173 SA1339 SA1038 SA2007 SA0671 SA1576 SA2434 SA1941 Glucosamine-6-phosphate isomerase, putative Phytoene dehydrogenase CBS domain protein Pyridine...”
- Effect of a glucose impulse on the CcpA regulon in Staphylococcus aureus
Seidl, BMC microbiology 2009 - “...*SA2295 NWNM_2403 gntR gluconate operon transcriptional repressor 1.5 3.2 SA2321 NWMN_2432 hypothetical protein 0.1 2.5 SA2434 NWNM_2540 PTS system, fructose-specific IIABC component 1.2 0.4 SA2435 NWNM_2541 pmi mannose-6-phosphate isomerase 1.2 0.4 1 Genes with parallel glucose-mediated regulation in wild-type and mutant but with different ratios, genes...”
- “...PTS system, mannitol specific IIBC component 6.4 0.2 *SA2293 NWNM_2401 gntP gluconate permease 0.7 2.5 SA2434 NWNM_2540 PTS system, fructose-specific IIABC component 1.2 0.4 a Cellular main roles are in accordance with the N315 annotation of the DOGAN website [ 26 ] and/or the KEGG website...”
- Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus
Donat, Journal of bacteriology 2009 - “...SA2079 SA2132 SA2143 SA2200 SA2202 SA2253 SA2302 SA2434 vraF vraG oppB oppD oppF oppA mtlF fhuD2 Change (fold)a Xanthine phosphoribosyltransferase...”
- Reporter metabolite analysis of transcriptional profiles of a Staphylococcus aureus strain with normal phenotype and its isogenic hemB mutant displaying the small-colony-variant phenotype
Seggewiss, Journal of bacteriology 2006 - “...0.00 0.01 2.19 0.38 2.04 SA0531 SA0417 SA0655 SA0374 SA2053 SA2434 0.01 0.01 0.01 0.01 0.01 0.02 2.20 2.15 2.25 0.39 2.06 2.31 a b Description PTS enzyme II,...”
- Global regulation of Staphylococcus aureus genes by Rot
Saïd-Salim, Journal of bacteriology 2003 - “...2612 2860 2926 3017 3078 3373 3768 SA2218 SA1437 SA2434 SA1725 SA1093 SA0989 SA0663 SA0127 SA0165 SA0904 SA0850 4061 4069 4530 5131 SA1007 SA0123 SA0710 SA2297...”
SAS2527 putative PTS transport system, IIABC component from Staphylococcus aureus subsp. aureus MSSA476
38% identity, 16% coverage
SPV1_10214 PTS IIA-like nitrogen-regulatory protein PtsN from Mariprofundus ferrooxydans PV-1
34% identity, 70% coverage
- Mariprofundus ferrooxydans PV-1 the first genome of a marine Fe(II) oxidizing Zetaproteobacterium
Singer, PloS one 2011 - “...M. ferrooxydans [1] , however PV-1 possesses a predicted operon (SPV1_t10271, SPV1_10194, SPV1_10199, SPV1_10204, SPV1_10209, SPV1_10214, SPV1_10219, SPV1_10224, SPV1_10229, SPV1_10234, SPV1_, SPV1_10239) encoding for a phosphoenolypyruvate-dependent sugar phosphotransferase system (PTS), which is the major carbohydrate transport system in bacteria [37] . The PTS enzyme II is...”
SGO_1113 PTS system, fructose specific IIABC components from Streptococcus gordonii str. Challis substr. CH1
37% identity, 20% coverage
- Transcriptome analysis of Streptococcus gordonii Challis DL1 indicates a role for the biofilm-associated fruRBA operon in response to Candida albicans
Jesionowski, Molecular oral microbiology 2016 - “...4.0 0.002 SGO_1111 Phosphotransferase system repressor fruR Energy metabolism/Biosynthesis and degradation of polysaccharides 3.4 0.002 SGO_1113 PTS system, fructose specific IIABC components fruA Regulatory functions/Other 3.4 0.001 SGO_0702 hypothetical protein Hypothetical 3.1 0.002 SGO_1109 aspartate carbamoyltransferase catalytic subunit pyrB Purines, pyrimidine, nucleosides, and nucleotides/Pyrimidine ribonucleotide biosynthesis...”
- “...Avg. Expression Potentially Up-regulated by Microarray analysis SGO_1111 fruR 6.5 1.98 SGO_1112 fruB 9.05 0.64 SGO_1113 fruA 3.75 0.92 SGO_0701 hup 0.95 0.21 SGO_0702 Hypothetical 1.95 0.35 Potentially Down-regulated by Microarray analysis SGO_1593 arcA 0.6 0.28 SGO_1592 arcB 0.6 0.28 SGO_1757 glmS 0.5 0.14 a Values...”
llmg_1568 PTS system, fructose specific IIBC components from Lactococcus lactis subsp. cremoris MG1363
33% identity, 22% coverage
- Further Elucidation of Galactose Utilization in Lactococcus lactis MG1363
Solopova, Frontiers in microbiology 2018 - “...llmg_0446 msmK 22.3 16.1 Multiple sugar-binding transport ATP-binding protein llmg_1569 fruC 4.5 1.1 Tagatose-6-phosphate kinase llmg_1568 fruA 3.8 1.3 PTS system, fructose specific IIBC components llmg_1570 fruR 3.1 1.3 Fructose operon transcriptional repressor Column Growth phase I shows fold upregulation of genes in cells harvested during...”
- Towards enhanced galactose utilization by Lactococcus lactis
Neves, Applied and environmental microbiology 2010 - “...llmg_0867 llmg_0963 llmg_1045 (ptbA) llmg_1244 llmg_1426 (yleD) llmg_1568 (fruA) llmg_pseudo54 (yidB) Familya VOL. 76, 2010 GALACTOSE METABOLISM IN L. LACTIS...”
BSQ49_00330 fructose-specific PTS transporter subunit EIIC from Liquorilactobacillus hordei
36% identity, 20% coverage
KQ76_13845, RDJ18_RS14335 fructose-specific PTS transporter subunit EIIC from Staphylococcus aureus
SAR2720 putative PTS transport system, IIABC component from Staphylococcus aureus subsp. aureus MRSA252
38% identity, 16% coverage
- Multiomics analysis of Staphylococcus aureus ST239 strains resistant to virulent Herelleviridae phages
Kornienko, Scientific reports 2024 - “...fructose into the cell at both early and late stages (RDJ18_RS00835, RDJ18_RS01075, RDJ18_RS01200, RDJ18_RS03920, RDJ18_RS13495, RDJ18_RS14335) (Table S4 ). The key genes encoding enzymes involved in glycolysis exhibited upregulation: pyruvate kinase (RDJ18_RS06570), L-lactate dehydrogenase (RDJ18_RS01065), phosphoglycerate kinase (RDJ18_RS04310), triose-phosphate isomerase (RDJ18_RS04315), and 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (RDJ18_RS04320)....”
- Unveiling Co-Infection in Cystic Fibrosis Airways: Transcriptomic Analysis of Pseudomonas aeruginosa and Staphylococcus aureus Dual-Species Biofilms
Magalhães, Frontiers in genetics 2022 - “...transport and metabolism 118.54 8.20E-04 KQ76_13345 Membrane protein [R] General functional prediction only 95.65 1.08E-02 KQ76_13845 PTS mannose transporter subunit IIABC [G] Carbohydrate transport and metabolism 78.44 0.00 KQ76_11410 6-Phospho-beta-galactosidase [G] Carbohydrate transport and metabolism 48.05 6.29E-09 KQ76_13300 Hypothetical protein [S] Function unknown 45.09 8.44E-12 KQ76_13275...”
- The Staphylococcus aureus response to unsaturated long chain free fatty acids: survival mechanisms and virulence implications
Kenny, PloS one 2009 - “...tagatose-bisphosphate aldolase 3.13 6.59E-03 SAR2262 putative uridylyltransferase 2.27 8.35E-03 SAR2579 gtaB putative uridylyltransferase 2.63 6.84E-03 SAR2720 putative PTS system component 4.17 3.54E-03 SAR2721 pmi mannose-6-phosphate isomerase 3.57 2.76E-03 Cell Wall Synthesis SAR0228 putative glutamine amidotransferase class-I 2.13 2.08E-03 SAR0257 lytS autolysin sensor kinase protein 3.33 3.00E-03...”
- “...and phosphorylation of glucose, mannose, maltose and galactitol, namely SAR0235 , SAR1777 ( pfkA ), SAR2720 , SAR2721 ( pmi ), SAR0242 and SAR0263 were also down-regulated. This could indicate an alteration of central metabolism via the action of the linoleic acid. Here, in concert with...”
RSAU_002484 fructose-specific PTS transporter subunit EIIC from Staphylococcus aureus subsp. aureus 6850
38% identity, 16% coverage
DQM59_RS06275 fructose-specific PTS transporter subunit EIIC from Streptococcus mutans
37% identity, 19% coverage
- Adaptation to simulated microgravity in Streptococcus mutans
Fernander, NPJ microgravity 2022 - “...MG2 MG3 MG4 Annotation Description 42 0.06 G88A rpoC 63 0.20 G123C ccpA 0.12 Y636* DQM59_RS06275 0.12 A78V DQM59_RS04610 (eno) 1.00 G88A rpoC 0.06 E105G rpoC 0.12 E55D DQM59_RS10195 (sprV) 0.11 L22I ylqF 100 0.14 intergenic (+181/+120) asnS / DQM59_RS04320 0.20 G77R DQM59_RS00400 0.84 intergenic (381/244)...”
str0401 PTS fructose-specific enzyme IIABC components, truncated from Streptococcus thermophilus CNRZ1066
42% identity, 69% coverage
VC1826 PTS system, fructose-specific IIABC component from Vibrio cholerae O1 biovar eltor str. N16961
35% identity, 23% coverage
- A mannose-sensing AraC-type transcriptional activator regulates cell-cell aggregation of Vibrio cholerae
Lee, NPJ biofilms and microbiomes 2022 - “...carbohydrates, including glucose, fructose, mannose, and N-acetylglucosamine 12 15 . The PTS transporter encoded by VC1826 has been annotated as a putative fructose PTS transporter as it comprises the fructose-family EIIABC domains. However, recent reports suggest that VC1826 is a mannose PTS transporter as the deletion...”
- “...13 . According to the transcriptome analysis of V. cholerae , the transcription level of VC1826 was significantly higher than that of genes encoding other PTS transporters during infection in rabbits and mice 16 . Moreover, a transposon insertion mutation in VC1826 decreases virulence in the...”
- Vibrio cholerae FruR facilitates binding of RNA polymerase to the fru promoter in the presence of fructose 1-phosphate
Yoon, Nucleic acids research 2021 - “...then channeled towards glycolysis following the activity of 1-phosphofructokinase (FruK) to yield fructose 1,6-bisphosphate (FBP). VC1826 was also shown to transport fructose ( 28 ). ( B ) Growth curves of in-frame deletion mutants of fruB , fruK , fruA , and fruR in M9 medium...”
- “...(TECAN). ( C ) The relative mRNA expression of fru operon genes, fruR , and VC1826 in the wild-type V. cholerae N16961 (left) and a fruR mutant (right) grown on fructose. The mRNA expression levels of indicated genes are shown as relative values (log 2 scale)...”
- Identification of Small Molecule Inhibitors of the Pathogen Box against Vibrio cholerae
Kim, Microbiology spectrum 2021 - “...was genes involved in carbon metabolism/transport. A few of the most downregulated genes, vc1820 , vc1826 , and vc1821 , are involved in the uptake of fructose. Iron homeostasis/transport showed the most upregulated number of genes along with ribosomal proteins ( Fig.2A ). These include vca0676...”
- Cra and cAMP Receptor Protein Have Opposing Roles in the Regulation of fruB in Vibrio cholerae
Beck, Journal of bacteriology 2021 - “...-specific components in V. cholerae are encoded by genes located at two distinct loci. First, VC1826 encodes an EIIABC fusion protein that is capable of both fructose and mannose transport ( 7 , 11 ). VCA0516, VCA0517, and VCA0518 make up the second locus encoding PTS...”
- Systematic genetic dissection of PTS in Vibrio cholerae uncovers a novel glucose transporter and a limited role for PTS during infection of a mammalian host
Hayes, Molecular microbiology 2017 - “...sources as well as redundancy amongst PTS transporters. For example, we find that VC2013 and VC1826 are independently capable of supporting growth in minimal medium supplemented with mannose as the sole carbon source ( Fig. 1D and Table 1 ) ( Houot et al ., 2010a...”
- “...such, also supports growth on glucose as the sole carbon source ( Table 1 ). VC1826 on the other hand is also capable of supporting growth when fructose is supplied as the sole carbon source ( Fig. 1E and Table 1 ). Thus, this analysis has...”
- A novel phase variant of the cholera pathogen shows stress-adaptive cryptic transcriptomic signatures
Lambert, BMC genomics 2016 - “...phosphotransferase system (PTS), including VCA0653 ( srcA ), which encodes a sucrose-specific PTS component and VC1826, which encodes a putative fructose-specific PTS component, were both down-regulated at least 30 fold on the N16961 to N16961R switch and then up-regulated in the N16961R to N16961SD switch (Additional...”
- Mannitol and the mannitol-specific enzyme IIB subunit activate Vibrio cholerae biofilm formation
Ymele-Leki, Applied and environmental microbiology 2013 - “...EII components of the PTS. In particular, VC1826 encoding the IIABC mannose permease (EIIMan), mtlA (VCA1045) encoding the IIABC mannitolspecific transporter...”
- Phase variable O antigen biosynthetic genes control expression of the major protective antigen and bacteriophage receptor in Vibrio cholerae O1
Seed, PLoS pathogens 2012 - “...hereafter designated manA-2 . manA-2 is located immediately downstream of a mannose permease encoded by VC1826 [46] , but is not linked to the O1 antigen biosynthetic cluster. manA and manA-2 are 65% identical at the nucleotide level over 70% of their sequence, and at the...”
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USA300HOU_2641 possible PTS family fructose/mannitol (fru) porter component IIABC from Staphylococcus aureus subsp. aureus USA300_TCH1516
38% identity, 16% coverage
SSA_1082 PTS system, fructose specific II ABC components, putative from Streptococcus sanguinis SK36
36% identity, 20% coverage
SCO3196 fructose-specific permease from Streptomyces coelicolor A3(2)
41% identity, 18% coverage
F5VVS9 PTS system protein fructose-specific EIIABC component from Streptococcus oralis SK255
37% identity, 20% coverage
SMUGS5_03875 fructose-specific PTS transporter subunit EIIC from Streptococcus mutans GS-5
39% identity, 17% coverage
- D‑Tagatose inhibits the growth and biofilm formation of Streptococcus mutans
Hasibul, Molecular medicine reports 2018 - “...EII-glu/man-F ctaaagctgaccgtatcattgttg 111 EII-glu/man-R attggtacaacattggccttgaca SMUGS5_09220 Maltose/Glucose-specific PTS subunit IIABC EII-mal/glu-F acacaatatctttggcggtaaga 105 EII-mal/glu-R cgaaaccagagataataccaacg SMUGS5_03875 Fructose-specific PTS subunit IIABC EII-fru-F tgatacttttaaggctgggatca 116 EII-fru-R aaaagaaccgttgcttcctttac SMUGS5_08275 Sucrose-specific PTS subunit IIABC EII-sucF ggtgtctttggtgctctgtattc 113 EII-suc-R gtcaccatgaccagtaccatttt SMUGS5_R09892 16S ribosomal RNA 16SF cctacgggaggcagcagtag 101 16SR caacagagctttacgatccgaaa...”
HMPREF0397_0483, HMPREF0397_RS07860 fructose-specific PTS transporter subunit EIIC from Fusobacterium nucleatum subsp. nucleatum ATCC 23726
36% identity, 20% coverage
LIC12955 nitrogen regulatory IIA protein from Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
28% identity, 99% coverage
- Core genome sequencing and genotyping of Leptospira interrogans in clinical samples by target capture sequencing
Grillova, BMC infectious diseases 2023 - “...were distributed in 22 different genes (Table 3 ). SNPs in genes LIC11311, LIC13481 and LIC12955 were conserved in most if not all samples (Table 3 ); other SNPs were sample specific. Of the identified mutations in the core genes, 9 were synonymous and 13 were...”
- “...623 20 623 Genes showing mutations LIC11335 LIC11374 LIC12764 LIC11455 LIC11604 LIC11518 LIC11570 LIC11791 LIC12870 LIC12955 LIC10974 LIC13481 Sample groL fliR ilvB mfd pulD recG rplB fruA fadA ycfH 1 12 30 721 2 666 30 721 3 721 4 30 721 8 1373 30 721...”
FN1441 PTS system, fructose-specific IIABC component from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
42% identity, 16% coverage
- Fusobacterium nucleatum extracellular vesicles are enriched in colorectal cancer and facilitate bacterial adhesion
Zheng, Science advances 2024 - “...on recipient cell surfaces. Given its ability to facilitate F. nucleatum autoaggregation through interaction with FN1441, the presence of FomA on CRC cell surfaces presents a target for bacterial adhesion. Collectively, the findings unveil a mechanism used by EVs to prepare a niche conducive for bacterial...”
- “...that FomA mediates autoaggregation of F. nucleatum likely through its interaction with another surface protein FN1441, the retention of FomA on the surface of CRC cells serves as an adhesive target for F. nucleatum . Together, we revealed that the enrichment of FnEVs in CRC promotes...”
- Oral Pathobiont-Derived Outer Membrane Vesicles in the Oral-Gut Axis
Catalan, International journal of molecular sciences 2024 - “...surface upon membrane fusion, enhancing Fn adhesion and autoaggregation by binding to the surface protein FN1441 (FruA). This adhesion was significantly reduced when using OMVs from a FomA-deficient Fn mutant strain [ 25 ]. FomA-containing Fn OMVs are also enriched in clinical CRC samples, further promoting...”
- Proteomics of Fusobacterium nucleatum within a model developing oral microbial community
Hendrickson, MicrobiologyOpen 2014 - “...Thompson 1990 ) and the ATCC 25586 genome encodes for a fructose-specific PTS component homolog (FN1441). Because of the amino acid dependence, glucose and galactose were not utilized strongly in the absence of amino acids. In the presence of amino acids, F. nucleatum ATCC 10953 utilized...”
TC 4.A.2.1.7 / Q1LZ59 The fructose inducible fructose/xylitol porter, FruI from Streptococcus mutans UA159 (see paper)
Q8DUN3 Inducible fructose permease from Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
SMU_872 fructose-specific PTS transporter subunit EIIC from Streptococcus mutans UA159
39% identity, 17% coverage
- substrates: Xylitol, fructose
- Strategic protein target analysis for developing drugs to stop dental caries
Horst, Advances in dental research 2012 - “...Q00749; Fig. 2b), fructose phosphotransferase (Q8DUN3), purine nucleoside phosphorylase (Q8DTU4), glycogen synthase (Q8CWX0), signal recognition particle...”
- Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability
Naka, Frontiers in cellular and infection microbiology 2022 - “...subunit IIBC 1027880 SMU_312 3.057 SMU_313 PTS system sorbitol-specific transporter subunit IIA 1029420 SMU_313 2.925 SMU_872 PTS system fructose-specific transporter subunit IIABC 1028238 SMU_872 2.920 copA Copper-transporting ATPase 1028623 SMU_426 2.886 nrgA Ammonium transporter 1028890 SMU_1658 2.865 SMU_1006 ABC transporter ATP-binding protein 1028342 SMU_1006 2.842 SMU_998...”
- Protein Interactomes of Streptococcus mutans YidC1 and YidC2 Membrane Protein Insertases Suggest SRP Pathway-Independent- and -Dependent Functions, Respectively
Lara, mSphere 2021 - “...protease HtrA (SMU_2164) Putative carbonic anhydrase precursor (SMU_1595) Putative PTS system, fructose-specific enzyme IIABC component (SMU_872) Putative PTS system, mannose-specific component IID (SMU_1879) Putative deacetylase (SMU_623c) Hypothetical protein (SMU_591c)* Hemolysin (SMU_1693) Conserved hypothetical protein, glycosyl transferase (SMU_834) Putative ABC transporter, ATP-binding protein ComA (SMU_286) Hypothetical protein...”
- MGcV: the microbial genomic context viewer for comparative genome analysis
Overmars, BMC genomics 2013 - “...the view shows that the operon showing the strongest response (consisting of SMU_870, SMU_871 and SMU_872) is not preceded by a putative binding-site and therefore probably is regulated indirectly. In fact, this operon encodes a PTS system for which no functional relation with nitrogen is described....”
VPA1420 PTS system, fructose-specific IIABC component from Vibrio parahaemolyticus RIMD 2210633
33% identity, 24% coverage
- Insights into Vibrio parahaemolyticus CHN25 response to artificial gastric fluid stress by transcriptomic analysis
Sun, International journal of molecular sciences 2014 - “...IIABC component VPA1422 Chn25A_1310 2.2688 Nitrogen regulatory IIA component VPA1421 Chn25A_1309 2.3425 Fructose-specific IIB component VPA1420 Chn25A_1308 2.8724 Fructose-specific IIABC component VPA0501 Chn25A_1196 2.6299 Mannitol-specific enzyme II component VPA0500 Chn25A_1197 2.6362 Mannitol-specific enzyme II component VPA0297 Chn25A_0302 8.029 fructose-specific IIBC component VPA0298 Chn25A_0303 2.8846 Fructose-specific IIA...”
NMB0736 PTS system, nitrogen regulatory IIA protein from Neisseria meningitidis MC58
34% identity, 95% coverage
- Transcriptomic buffering of cryptic genetic variation contributes to meningococcal virulence
Ampattu, BMC genomics 2017 - “...misR Response regulator 0.89 1.09 n. s n. s. 1.64 n. s. 1.30 n. s. NMB0736 ptsN Nitrogen regulator IIA 1.41 n. s. n. a. (e) n. a. n. a. n. a. n. a. n. a. NMB1267 - Tyrosine-phosphatase 1.50 n. s. n. s. n. s....”
- The Phosphocarrier Protein HPr Contributes to Meningococcal Survival during Infection
Antunes, PloS one 2016 - “...components, one of the mannose and the other of the fructose PTS family (NMB2046 and NMB0736, respectively). However, the PTS of meningococcus is incomplete because it lacks both the B and C domains necessary for the uptake of carbohydrates by this system. HPr not only plays...”
- Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis
Ren, BMC structural biology 2005 - “...distribution, and reproduction in any medium, provided the original work is properly cited. Background The NMB0736 gene of Neisseria meningitidis serogroup B strain MC58 encodes the putative nitrogen regulatory protein, IIA Ntr (abbreviated to NM-IIA Ntr ). The homologous protein present in Escherichia coli is implicated...”
- “...putative nitrogen regulatory protein IIA Ntr of N. meningitidis (abbreviated to NM-IIA Ntr ) (Gene NMB0736), the sequence of which is highly conserved amongst the Neisseria spp. In Escherichia coli , the IIA Ntr gene ( ptsN ) is located within the sigma-54 factor coding operon,...”
blr0725 nitrogen regulatory IIA protein from Bradyrhizobium japonicum USDA 110
38% identity, 78% coverage
lp_2097 fructose-specific PTS transporter subunit EIIC from Lactiplantibacillus plantarum WCFS1
lp_2097 fructose PTS, EIIABC from Lactobacillus plantarum WCFS1
40% identity, 20% coverage
- Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study
Petrov, Genes 2021 - “...in Ym1 FC * in 8-1 lp_0230, pts2CB PTS mannitol transporter subunit IICBA +6.48 3.22 lp_2097, fruA PTS transporter subunit EIIA +4.35 +2.48 lp_3654, pts38C PTS sorbitol transporter subunit IIC +2.29 NC lp_0286, pts6C PTS cellobiose transporter subunit IIC +2.55 NC lp_2531, pts18CBA PTS transporter subunit...”
- Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts
Santamaría, Frontiers in microbiology 2019 - “...lp_0286 , pts20A or lp_2780 , pts20B or lp_2781 ) and fructose ( pts16ABC or lp_2097 ) were all induced in presence of OLE. In addition, the maltose proton transporter ( malT or lp_1729 ) was also up-regulated. In contrast, two PTS systems involved in the...”
- Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays
Saulnier, Applied and environmental microbiology 2007 - “...lp_1273 lp_1274 lp_1398 lp_1481 lp_1498 lp_1499 lp_2096 lp_2097 lp_2151 lp_2152 lp_2153 lp_2154 lp_2371 lp_2757 lp_2684 lp_2776 lp_2794 lp_2920 lp_2969 lp_3010...”
HGB56_02940 fructose-specific PTS transporter subunit EIIC from Lactiplantibacillus plantarum
40% identity, 20% coverage
- The Carbohydrate Metabolism of Lactiplantibacillus plantarum
Cui, International journal of molecular sciences 2021 - “...LP-F1 contained multiple phosphotransferase systems (PTSs) responsible for fructose transport, which were encoded by fruA (HGB56_02940), fruA1-fruA2-fruB (HGB56_07725, HGB56_07730, HGB56_07735), pts9ABCD (HGB56_11950, HGB56_11955, HGB56_11960) and pts10AB (HGB56_11975, HGB56_11980) genes, respectively ( Supplementary Table S3-4 ). Fructose enters cells in the form of 6-phosphate-fructose through these PTSs,...”
RL0425 putative nitrogen regulatory IIA protein from Rhizobium leguminosarum bv. viciae 3841
38% identity, 80% coverage
SEQ_1299 fructose-specific phosphotransferase system (PTS), IIABC component from Streptococcus equi subsp. equi 4047
37% identity, 18% coverage
SUB0750 fructose-specific phosphotransferase system (PTS), IIABC component from Streptococcus uberis 0140J
36% identity, 18% coverage
Pcar_1936 phosphotransferase system enzyme IIA, regulates nitrogen metabolism from Pelobacter carbinolicus str. DSM 2380
38% identity, 71% coverage
L21SP2_0865 cation:proton antiporter from Salinispira pacifica
38% identity, 17% coverage
- Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat
Ben, Standards in genomic sciences 2015 - “...encoding phosphocarrier proteins (L21SP2_2173 and L21SP2_3294), enzyme I (L21SP2_1343 and L21SP2_3295), and enzyme II (L21SP2_0100, L21SP2_0865, L21SP2_3408, and L21SP2_3409) of a putative PTS transport mechanism were detected. In addition, genes encoding a TRAP-type dicarboxylate transporter (L21SP2_1277 and L21SP2_1278) were present. The assimilation of peptides, which had...”
Fisuc_1572 putative PTS IIA-like nitrogen-regulatory protein PtsN from Fibrobacter succinogenes subsp. succinogenes S85
33% identity, 84% coverage
- Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85
Wu, Scientific reports 2017 - “...these 46 genes, the most up-regulated genes were Fisuc_1985 (1.4 fold at pH 5.65) and Fisuc_1572 (1.3 fold at pH 5.65). Fisuc_1985 is an annotated glycosidase that is involved in glycosaminoglycan degradation and beta-Lactam resistance. Conversely, an orthologous gene (Fisuc_1751) in the same category that was...”
SPy0855 putative fructose-specific enzyme II, PTS system BC component from Streptococcus pyogenes M1 GAS
36% identity, 20% coverage
LA_0632 phosphoenolpyruvate-dependent enzyme IIA component of the PTS system from Leptospira interrogans serovar lai str. 56601
29% identity, 84% coverage
VSAL_II0894 fructose-specific PTS transporter subunit EIIC from Aliivibrio salmonicida LFI1238
VSAL_II0894 PTS system, IIBC component from Vibrio salmonicida LFI1238
33% identity, 22% coverage
Spy49_0674 PTS system, fructose-specific IIABC component from Streptococcus pyogenes NZ131
36% identity, 20% coverage
NGK_0461 PTS system, nitrogen regulatory IIA protein from Neisseria gonorrhoeae NCCP11945
B4RK01 PTS system, nitrogen regulatory IIA protein from Neisseria gonorrhoeae (strain NCCP11945)
33% identity, 95% coverage
- Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability
Diao, Frontiers in microbiology 2020 - “...WHOF_00223 Glycosyltransferase PglE G 2 N 3.85 A0A1P8DWD0 mtrR MtrR K 0 N 2.96 B4RK01 NGK_0461 PTS system, nitrogen regulatory IIA protein GT 0 N 1.79 B4RK88 NGK_0548 Putative oxidoreductase, NAD(P)Hflavin C 0 N 7.12 D6H8D7 NGMG_01320 Uncharacterized protein C 0 N 1.65 D6H8V1 NGMG_01484 Succinate...”
- Comparative Proteomics of Extended-Spectrum Cephalosporin-Resistant Neisseria gonorrhoeae Isolates Demonstrates Altered Protein Synthesis, Metabolism, Substance Transport, and Membrane Permeability
Diao, Frontiers in microbiology 2020 - “...A0A1D3J066 WHOF_00223 Glycosyltransferase PglE G 2 N 3.85 A0A1P8DWD0 mtrR MtrR K 0 N 2.96 B4RK01 NGK_0461 PTS system, nitrogen regulatory IIA protein GT 0 N 1.79 B4RK88 NGK_0548 Putative oxidoreductase, NAD(P)Hflavin C 0 N 7.12 D6H8D7 NGMG_01320 Uncharacterized protein C 0 N 1.65 D6H8V1 NGMG_01484...”
YP_003171105 PTS system, mannitol/fructose-specific IIABC component from Lactobacillus rhamnosus GG
40% identity, 19% coverage
- Growth Mode and Carbon Source Impact the Surfaceome Dynamics of Lactobacillus rhamnosus GG
Savijoki, Frontiers in microbiology 2019 - “...secretion (MFP/HlyD), amino acid (OppA)/metal ion intake/output proteins (YP_003172665.1, YP_003169947.1, YP_003169947.1, YP_003170942.1, YP_003172169.1, YP_003170075.1, and YP_003171105) and two penicillin-binding proteins (PbP2 and PbP2X-like/FtsI). In addition, two phosphoglycerol transferase (MdoB) paralogs of different sizes (78 and 84 kDa) and an r-protein, RplX, were detected as candidate moonlighting...”
VPAR_RS08070 fructose-specific PTS transporter subunit EIIC from Veillonella parvula DSM 2008
35% identity, 21% coverage
A0KI59 protein-N(pi)-phosphohistidine--D-fructose phosphotransferase from Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049)
36% identity, 23% coverage
ECs4826 fructose-specific PTS system enzyme IIA component from Escherichia coli O157:H7 str. Sakai
33% identity, 75% coverage
L21SP2_0100 PTS sugar transporter subunit IIA from Salinispira pacifica
36% identity, 42% coverage
- Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat
Ben, Standards in genomic sciences 2015 - “...genes encoding phosphocarrier proteins (L21SP2_2173 and L21SP2_3294), enzyme I (L21SP2_1343 and L21SP2_3295), and enzyme II (L21SP2_0100, L21SP2_0865, L21SP2_3408, and L21SP2_3409) of a putative PTS transport mechanism were detected. In addition, genes encoding a TRAP-type dicarboxylate transporter (L21SP2_1277 and L21SP2_1278) were present. The assimilation of peptides, which...”
Dpo_3c02590 PTS sugar transporter subunit IIA from Desulfotignum phosphitoxidans DSM 13687
34% identity, 66% coverage
GMI18_RS08780 fructose-specific PTS transporter subunit EIIC from Spiroplasma citri
36% identity, 17% coverage
SP_0877 fructose-specific PTS transporter subunit EIIC from Streptococcus pneumoniae TIGR4
36% identity, 18% coverage
- A Nonadjuvanted Whole-Inactivated Pneumococcal Vaccine Induces Multiserotype Opsonophagocytic Responses Mediated by Noncapsule-Specific Antibodies
David, mBio 2022 - “...20,514.81,842.74 5,411.27557.36 1.923 SP_0629 VanY domain-containing protein 17,791.131,923.57 11,916.271,183.09 0.578 SP_0845 Lipoprotein 17,436.81,509.69 18,895.932,571.16 0.116 SP_0877 fruA PTS system, fructose specific IIABC components 16,762.471,323.25 12,392.272,455.32 0.436 SP_1368 psr Psr protein 16,422.132,437.2 20,221.273,117.34 0.300 SP_0730 spxB Pyruvate oxidase 15,773.81,840.67 3,601.27212.76 2.131 SP_1872 piuA Iron-compound ABC transporter, iron-compound-binding...”
- Polyamine Synthesis Effects Capsule Expression by Reduction of Precursors in Streptococcus pneumoniae
Ayoola, Frontiers in microbiology 2019 - “...Choline binding protein I 1.5 pcpA SP_2136 Choline binding protein PcpA 2.3 Carbon utilization pathways SP_0877 SP_0877 PTS system, fructose-specific IIABC components 2.6 SP_0645 SP_0645 Putative PTS system, IIA component 3.1 SP_0646 SP_0646 Putative PTS system, IIB component 2.6 SP_0647 SP_0647 Putative PTS system, IIC component...”
- “...in metabolic pathways catalyzing the conversion of galactose ( SP_0645-7 , lacDCBA ), fructose ( SP_0877 ), fucose ( fucA , SP_2167 ), and GlcNAc1P ( nagA , nagB ) to G3P with concomitant higher expression of transketolase C and N subunits ( tktC , tktN...”
- ManLMN is a glucose transporter and central metabolic regulator in Streptococcus pneumoniae
Fleming, Molecular microbiology 2016 - “...transporter (SP_0061-3; PTS) and one predicted fructose transporter (SP_0877; PTS), encoded in the TIGR4 genome. There is only one predicted lactose transporter...”
- “...(SP_0061-3), the lac PTS (SP_1185-6) and the fru PTS (SP_0877). In contrast to the manLMN deletion strain, inactivation of the GlcNAc/gal, lac or fru PTS had no...”
Q8DWE6 protein-Npi-phosphohistidine-D-fructose phosphotransferase (subunit 1/2) (EC 2.7.1.202) from Streptococcus mutans serotype c (see paper)
TC 4.A.2.1.8 / Q1LZ68 Constitutive fructose permease aka FruD, component of The constitutive fructose porter FruC/FruD from Streptococcus mutans UA159 (see paper)
SMU_115 PTS sugar transporter subunit IIA from Streptococcus mutans UA159
29% identity, 90% coverage
FrvA / b3900 putative PTS enzyme IIA component FrvA from Escherichia coli K-12 substr. MG1655 (see paper)
TC 4.A.2.1.25 / P32155 Fructose-like phosphotransferase enzyme IIA component, component of Fructose-like PTS Enzyme II complex, FrvA (IIA of 148 aas) - FrvB (IIBC of 483 aas and 9 predicted TMSs) from Escherichia coli (strain K12)
33% identity, 75% coverage
TC 4.A.2.1.16 / Q9RMF5 Fructose-specific Enzyme IIABC from Spiroplasma citri
36% identity, 17% coverage
BH00180 Nitrogen regulatory IIA from Bartonella henselae str. Houston-1
37% identity, 74% coverage
ZMO0037 putative PTS IIA-like nitrogen-regulatory protein PtsN from Zymomonas mobilis subsp. mobilis ZM4
40% identity, 79% coverage
SPD_0773 PTS system, fructose specific IIABC components from Streptococcus pneumoniae D39
36% identity, 18% coverage
- Glutamate Dehydrogenase (GdhA) of Streptococcus pneumoniae Is Required for High Temperature Adaptation
Gazioglu, Infection and immunity 2021 (secret) - Arachidonic Acid Stress Impacts Pneumococcal Fatty Acid Homeostasis
Eijkelkamp, Frontiers in microbiology 2018 - “...fructose phosphotransferase system (PTS) repressor (SPD_0771), phosphofructokinase (SPD_0772) and fructose PTS system enzyme II ABC (SPD_0773), was significantly upregulated (Table S2 ), corroborating previous studies of YycFG (Ng et al., 2003 ). Transcription of the yycF and yycG genes increased by 1.3- and 1.2-fold upon AA...”
- Panproteome-wide analysis of antibody responses to whole cell pneumococcal vaccination
Campo, eLife 2018 - “...0.039 (0.0850.071) 0.615 (0.1171.452) 0.283 (0.2310.730) 1.986 (1.4542.437) Both FruA Fructose PTS transporter protein FruA SPD_0773 1.044 (0.5681.389) 0.029 (0.125 - 0.002) 0.480 (0.1180.817) 0.335 (0.3220.360) 0.591 (0.4060.770) Linear mixed effects GalT Galactose-1-phosphate uridylyltransferase GalT SPD_1633 0.295 (0.2110.494) 0.024 (0.0550.047) 0.110 (0.220 - 0.034) 0.068 (0.0820.012)...”
- Autoinducer 2 Signaling via the Phosphotransferase FruA Drives Galactose Utilization by Streptococcus pneumoniae, Resulting in Hypervirulence
Trappetti, mBio 2017 - “...SPD_0771 lacR1 Lactose phosphotransferase system repressor 1.9 0.3 0.6 SPD_0772 fruB 1-Phosphofructokinase 1.9 0.3 0.5 SPD_0773 fruA PTS, fructose-specific IIABC components 1.7 0.4 0.7 c SPD_0900 asd Aspartate-semialdehyde dehydrogenase 1.6 SPD_0901 dapA Dihydrodipicolinate synthase 1.6 SDP_1004 gapN Glyceraldehyde-3-phosphate dehydrogenase 0.6 SPD_1256 Peptidase, U32 family protein 0.6...”
- Ascorbic acid-dependent gene expression in Streptococcus pneumoniae and the activator function of the transcriptional regulator UlaR2
Afzal, Frontiers in microbiology 2015 - “...protein, IID component 2.2 spd_0502 PTS system, beta-glucosides-specific IIABC components 2.3 spd_0503 6-Phospho-beta-glucosidase, BglA-2 2.9 spd_0773 PTS system, fructose specific IIABC components 2.4 spd_0888 Adhesion lipoprotein, AdcAII/LmB 3.2 spd_0889 Pneumococcal histidine triad protein D, PhtD 3.6 spd_0890 Pneumococcal histidine triad protein E, PhtE 4.4 spd_1038 Pneumococcal...”
- Transcriptional and metabolic effects of glucose on Streptococcus pneumoniae sugar metabolism
Paixão, Frontiers in microbiology 2015 - “...1.21 SPD_0561 PTS system, IIC component, putative 1.55 SPD_0661 exp5 PTS system, IIABC components 0.61 SPD_0773 PTS system, fructose specific IIABC components 0.36 SPD_1039 ptsI Phosphoenolpyruvate-protein phosphotransferase 0.49 SPD_1040 ptsH Phosphocarrier protein HPr 0.56 SPD_1047 lacE-2 PTS system, lactose-specific IIBC components 0.78 SPD_1057 PTS system, IIB...”
spr0780 Fructose specific-phosphotransferase system IIBC component from Streptococcus pneumoniae R6
36% identity, 18% coverage
A0J47_RS05670 fructose-specific PTS transporter subunit EIIC from Photobacterium damselae subsp. damselae
31% identity, 23% coverage
VIBHAR_05188 PTS system fructose-specific IIABC component from Vibrio harveyi ATCC BAA-1116
33% identity, 24% coverage
CCNA_03710 nitrogen regulatory IIA protein from Caulobacter crescentus NA1000
34% identity, 75% coverage
MSMEG_0085 PTS system, Fru family protein, IIABC components from Mycobacterium smegmatis str. MC2 155
38% identity, 16% coverage
RSUY_28680, RSUY_RS14025 PTS IIA-like nitrogen regulatory protein PtsN from Ralstonia solanacearum
35% identity, 70% coverage
- Transcriptomes of Ralstonia solanacearum during Root Colonization of Solanum commersonii
Puigvert, Frontiers in plant science 2017 - “...RSp0419 2.75083 siderophore biosynthesis protein RSUY_RS17050 RSUY_34890 RSp0421 2.94313 siderophore biosynthesis protein Nitrogen metabolism RSUY_RS14025 RSUY_28680 RSc0406 2.27 ptsN PTS IIA-like nitrogen-regulatory protein PtsN RSUY_RS17995 RSUY_36860 RSp0980 2.24 narL DNA-binding response regulator RSUY_RS11470 RSUY_23380 RSc2031 3.3167 ureE urease accessory protein UreE Transporters RSUY_RS20760 RSUY_42660 RSp1283 2.005142...”
- “...RSUY_34870 RSp0419 2.75083 siderophore biosynthesis protein RSUY_RS17050 RSUY_34890 RSp0421 2.94313 siderophore biosynthesis protein Nitrogen metabolism RSUY_RS14025 RSUY_28680 RSc0406 2.27 ptsN PTS IIA-like nitrogen-regulatory protein PtsN RSUY_RS17995 RSUY_36860 RSp0980 2.24 narL DNA-binding response regulator RSUY_RS11470 RSUY_23380 RSc2031 3.3167 ureE urease accessory protein UreE Transporters RSUY_RS20760 RSUY_42660 RSp1283...”
RSc0406 PUTATIVE NITROGEN REGULATORY IIA (ENZYME IIA-NTR) (PHOSPHOTRANSFERASE ENZYME II, A COMPONENT) TRANSCRIPTION REGULATOR PROTEIN from Ralstonia solanacearum GMI1000
35% identity, 70% coverage
- Transcriptomes of Ralstonia solanacearum during Root Colonization of Solanum commersonii
Puigvert, Frontiers in plant science 2017 - “...2.75083 siderophore biosynthesis protein RSUY_RS17050 RSUY_34890 RSp0421 2.94313 siderophore biosynthesis protein Nitrogen metabolism RSUY_RS14025 RSUY_28680 RSc0406 2.27 ptsN PTS IIA-like nitrogen-regulatory protein PtsN RSUY_RS17995 RSUY_36860 RSp0980 2.24 narL DNA-binding response regulator RSUY_RS11470 RSUY_23380 RSc2031 3.3167 ureE urease accessory protein UreE Transporters RSUY_RS20760 RSUY_42660 RSp1283 2.005142 porin...”
U876_15985 fructose-specific PTS transporter subunit EIIC from Aeromonas hydrophila NJ-35
37% identity, 20% coverage
BAB1_0160 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2:PTS IIA-like nitrogen-regulatory protein PtsN from Brucella melitensis biovar Abortus 2308
BMEI1786 NITROGEN REGULATORY IIA PROTEIN from Brucella melitensis 16M
33% identity, 97% coverage
- Transposon Sequencing of Brucella abortus Uncovers Essential Genes for Growth In Vitro and Inside Macrophages
Sternon, Infection and immunity 2018 - “...Change in R200 valuec ORFb BAB2_0678 BAB1_1517 BAB2_0118 BAB2_0143 BAB1_0160 BAB1_0638 Gene name rirA vtlR vjbR deoR1 ptsN glnE 2 h p.i. 5 h p.i. 24 h...”
- Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome
Lamontagne, BMC genomics 2010 - “...BAB1_0918 GatB/Yqey BAB1_1553 YchF BAB2_0177 YafB BAB1_0159 S30EA BAB1_0924 AccC BAB1_1613 Unknown BAB2_0186 Fumarate hydratase BAB1_0160 PtsN-like BAB1_0933 PCRF 2 BAB1_1645 DhaK-1 BAB2_0187 Unknown BAB1_0191 GABAtrnsam BAB1_0943 TyrS BAB1_1646 DhaK-2 BAB2_0191 HAD-like, BAB1_0204 AdhP BAB1_0949 SufC BAB1_1655 GabD subfamily IIA BAB1_0215 ThiE BAB1_0955 DeaD BAB1_1669 PAS...”
- Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics
He, Frontiers in cellular and infection microbiology 2012 - “...14979322 141 feuQ BMEI1336 Mice, macrophages, HeLa 14979322 142 ftcR BMEII0158 Mice 17056750 143 glnL BMEI1786 Macrophages 14979322 144 nodV BMEII0052 Mice, macrophages, HeLa 14979322 145 ntrY BMEI0867 Mice 10678941 146 pstP BMEI0190 Macrophages, HeLa 14979322 147 spotT BMEI1296 Macrophages, HeLa 14979322 148 vsrB BMEI1606 Mice,...”
SMc01141 PROBABLE NITROGEN REGULATORY IIA PROTEIN from Sinorhizobium meliloti 1021
37% identity, 68% coverage
FryA / b2383 putative PTS multiphosphoryl transfer protein FryA from Escherichia coli K-12 substr. MG1655 (see 4 papers)
fryA / RF|NP_416884 multiphosphoryl transfer protein 1 from Escherichia coli K12 (see paper)
b2383 fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component from Escherichia coli str. K-12 substr. MG1655
31% identity, 17% coverage
H16_A0384 phosphotransferase system mannitol/fructose-specific IIA domain protein (Ntr-type) from Ralstonia eutropha H16
H16_A0384 PTS IIA-like nitrogen regulatory protein PtsN from Cupriavidus necator H16
38% identity, 69% coverage
- The Modification of Regulatory Circuits Involved in the Control of Polyhydroxyalkanoates Metabolism to Improve Their Production
Velázquez-Sánchez, Frontiers in bioengineering and biotechnology 2020 - “...genes of the PTS Ntr , but has a homologous to ptsN (IIA Ntr , H16_A0384) ( Kaddor and Steinbchel, 2011 ; Kaddor et al., 2012 ). Inactivation of ptsN increased PHB accumulation, as in A vinelandii , and P . putida ( Kaddor and Steinbchel,...”
- Global changes in the proteome of Cupriavidus necator H16 during poly-(3-hydroxybutyrate) synthesis from various biodiesel by-product substrates
Sharma, AMB Express 2016 - “...). In REG-GB cultures, a serine kinase of the Hpr protein (H16_A0383) and phosphotransferase system (H16_A0384), related to alternate PEP-PTS, were identified, but not expressed at significantly greater levels at 48hpi with any of the substrates. Expression of proteins related to the -oxidation pathway As expected,...”
- Implications of various phosphoenolpyruvate-carbohydrate phosphotransferase system mutations on glycerol utilization and poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha H16
Kaddor, AMB Express 2011 - “...strain in media containing glycerol as a sole carbon source. Otherwise, the deletion of gene H16_A0384 ( ptsN , nitrogen regulatory EIIA component of PTS) seemed to largely compensate the effect of the deleted ptsMHI operon (49%, w/w, PHB). The involvement of the PTS homologous proteins...”
- “...H16_A0325* Histidine phosphocarrier protein HPr ptsI H16_A0326* Enzyme I component H16_A2203 H16_A2203* HPr-related phosphocarrier protein H16_A0384 ( ptsN ) H16_A0384* Nitrogen regulatory EIIA Ntr component; Mannitol/fructose-specific EIIA Mtl component nagF H16_A0311* Phosphocarrier protein, N -Acetylglucosamine-specific EI Nag -HPr Nag -EIIA Nag components nagE H16_A0312* N -Acetylglucosamine-specific...”
- Effects of homologous phosphoenolpyruvate-carbohydrate phosphotransferase system proteins on carbohydrate uptake and poly(3-Hydroxybutyrate) accumulation in Ralstonia eutropha H16
Kaddor, Applied and environmental microbiology 2011 - “...reduced PHB production in mutants lacking the H16_A2203, H16_A0384, frcACB, or nagFEC genes. In contrast, mutant H16 H16_A0384 accumulated 11.5% (wt/wt) more...”
- “...IIA component (EIIANtr) encoded by H16_A0384; an N-acetylglucosamine-specific IIBC protein-N(pi)-phosphohistidine-sugar phosphotransferase (EIIBCNag) encoded by...”
BR0161 PTS system, nitrogen regulatory IIA component from Brucella suis 1330
34% identity, 97% coverage
LJ0146 phosphoenolpyruvate-dependent sugar phosphotransferase system EIIABC, probable fructose specific from Lactobacillus johnsonii NCC 533
31% identity, 20% coverage
lmo2137 similar to PTS system, fructose-specific enzyme IIA component from Listeria monocytogenes EGD-e
30% identity, 96% coverage
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...2.61 Yes/down lmo2135 PTS fructose transporter subunit IIC lmo2136 lmo2136 PTS fructose transporter subunit IIB lmo2137 lmo2137 PTS fructose transporter subunit IIA lmo2259 3.02 Yes/up lmo2259 PTS beta-glucoside transporter subunit IIA lmo2335 5.14 Yes/down fruA PTS fructose transporter subunit IIABC lmo2373 1.30 Yes/up lmo2373 PTS beta-glucoside...”
- New Insights into the Lactic Acid Resistance Determinants of Listeria monocytogenes Based on Transposon Sequencing and Transcriptome Sequencing Analyses
Liu, Microbiology spectrum 2023 - “...fructose transporter subunit IIC 0.2246 1.88E-04 lmo2136 lmo2136 PTS fructose transporter subunit IIB 0.1801 8.24E-03 lmo2137 lmo2137 PTS fructose transporter subunit IIA 0.0825 1.19E-04 lmo2373 lmo2373 PTS beta-glucoside transporter subunit IIB 0.4827 5.21E-25 lmo2649 ulaA PTS system ascorbate transporter subunit IIC 0.0017 0.00E+00 lmo2650 lmo2650 MFS...”
- The transcriptional response of Listeria monocytogenes during adaptation to growth on lactate and diacetate includes synergistic changes that increase fermentative acetoin production
Stasiewicz, Applied and environmental microbiology 2011 - “...(fructose transporter genes lmo0357, lmo0426, and lmo2137 fruA), prophage genes (lmo2283, lmo2284, lmo2286, and lmo2293), zinccontaining alcohol dehydrogenase...”
- Probing the pan-genome of Listeria monocytogenes: new insights into intraspecific niche expansion and genomic diversification
Deng, BMC genomics 2010 - “...IIIA + - lmo2136 Similar to PTS system, fructose-specific enzyme IIB component IIIA + - lmo2137 Similar to PTS system, fructose-specific enzyme IIA component IIIA + - lmo2143 Similar to mannose-6-phosphate isomerase IIIA - - lmo2733 Similar to PTS system, fructose-specific IIABC component IIIA + 494...”
- “...but decayed or lost in LIII. For example, the fructose-like PTS components ( lmo2133 --- lmo2137 ) are conserved in all LI and LII genomes but completely lost in LIIIB and LIIIC (Figure 1 ). This operon was postulated to have been acquired by L. monocytogenes...”
AFE_3023 PTS sugar transporter subunit IIA from Acidithiobacillus ferrooxidans ATCC 23270
40% identity, 61% coverage
LMRG_00091 hypothetical protein from Listeria monocytogenes 10403S
lmo0398 similar to phosphotransferase system enzyme IIA from Listeria monocytogenes EGD-e
33% identity, 92% coverage
- Listeria monocytogenes 10403S Alternative Sigma-54 Factor σL Has a Negative Role on Survival Ability Under Bile Exposure
Boonmee, Frontiers in microbiology 2021 - “...regulated genes, 174 genes were defined as negatively regulated by L , of which the LMRG_00091- LMRG_00094 operon encoding fructose-specific PTS components showed lower transcript levels as low as 39 FCs. LMRG_01669 - LMRG_01672 involved in myo-inositol degradation, LMRG_02570 encoding a cellobiose-specific PTS component, and LMRG_01248-LMRG_01249...”
- “...sigL upregulated genes that were suppressed or downregulated. Among the upregulated gene in L background, LMRG_00091 gene, identified as L negatively regulated gene, was also upregulated as high as 61 FC. To identify potential reporter genes for L activities, selected sets of L -dependent genes were...”
- Listeria monocytogenes σA Is Sufficient to Survive Gallbladder Bile Exposure
Boonmee, Frontiers in microbiology 2019 - “...validation were (i) rpoB and bglA as housekeeping genes and (ii) gadT2 , plcA , LMRG_00091 , LMRG_01149 , LMRG_01669 , and LMRG_02283 for sigma A function. A list of TaqMan primers and probes designed by PrimerQuest (IDT DNA, Coralville, IA, United States) are shown in...”
- “...2.22 0.02 LMRG_01919 lmo2778 Hypothetical protein 2.05 0.03 LMRG_02808 lmo2132 Cyclic nucleotide-binding protein 2.08 0.00 LMRG_00091 lmo0398 LMRG_00091-LMRG_00095 PTS system, IIA component 2.51 0.01 LMRG_00092 lmo0399 PTS system, IIB component 3.52 0.00 LMRG_00352 lmo0665 LMRG_00351-LMRG_00353 Hypothetical protein 2.32 0.01 LMRG_00353 lmo0666 Domain-containing protein 2.12 0.01 LMRG_00369...”
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...lmo0373 PTS beta-glucoside transporter subunit IIC lmo0374 2.43 Yes/down lmo0374 PTS beta-glucoside transporter subunit IIB lmo0398 3.27 Yes/down 3.27 Yes/down lmo0398 PTS sugar transporter subunit IIA lmo0399 7.90 Yes/down lmo0399 PTS fructose transporter subunit IIB lmo0400 9.39 Yes/down lmo0400 PTS fructose transporter subunit IIC lmo0426 1.75...”
- Listeria monocytogenes σA Is Sufficient to Survive Gallbladder Bile Exposure
Boonmee, Frontiers in microbiology 2019 - “...0.02 LMRG_01919 lmo2778 Hypothetical protein 2.05 0.03 LMRG_02808 lmo2132 Cyclic nucleotide-binding protein 2.08 0.00 LMRG_00091 lmo0398 LMRG_00091-LMRG_00095 PTS system, IIA component 2.51 0.01 LMRG_00092 lmo0399 PTS system, IIB component 3.52 0.00 LMRG_00352 lmo0665 LMRG_00351-LMRG_00353 Hypothetical protein 2.32 0.01 LMRG_00353 lmo0666 Domain-containing protein 2.12 0.01 LMRG_00369 lmo0681...”
- Phosphotransferase system-dependent extracellular growth of listeria monocytogenes is regulated by alternative sigma factors σL and σH
Wang, Applied and environmental microbiology 2014 - “...regulation of other pts genes, including lmo0021, lmo0027, lmo0398 to lmo0400, lmo2733, lmo0631, and lmo2665 (13, 14). The growth of L. monocytogenes is...”
- Refinement of the Listeria monocytogenes σB regulon through quantitative proteomic analysis
Mujahid, Microbiology (Reading, England) 2013 - “...transcription of this gene. The Group D proteins Lmo0398 and Lmo0399 (annotated as fructose-specific PTS system IIA and IIB components) are also encoded by...”
- “...in the microarray study with the prfA* strain. lmo0398 and lmo0399 were previously proposed to play a role in sB-dependent regulation of carbohydrate metabolism...”
- Transcriptomic and phenotypic analyses identify coregulated, overlapping regulons among PrfA, CtsR, HrcA, and the alternative sigma factors sigmaB, sigmaC, sigmaH, and sigmaL in Listeria monocytogenes
Chaturongakul, Applied and environmental microbiology 2011 - “...inhibits expression of the fructose phosphotransferase system lmo0398 operon under salt stress but positively regulates transcription of these genes during...”
- Listeria monocytogenes {sigma}B has a small core regulon and a conserved role in virulence but makes differential contributions to stress tolerance across a diverse collection of strains
Oliver, Applied and environmental microbiology 2010 - “...4). Microarray analyses suggested that B-dependent transcription of lmo0398, which is similar to a gene encoding a phosphotransferase system (PTS) enzyme IIA...”
- “...lineages. Using TaqMan qRT-PCR, we found that transcription of lmo0398 was B dependent in both lineages I and II, as shown by significantly higher transcript...”
- Modulation of stress and virulence in Listeria monocytogenes
Chaturongakul, Trends in microbiology 2008 - “...38 ]. Several operons contributing to carbohydrate metabolism are B -dependent, including lmo0784 lmo0781 and lmo0398 lmo0400 , which encode mannose-specific and fructose-specific phosphotransferase systems, respectively [ 25 ]. In addition, the presence of a putative B -dependent promoter upstream of lmo2460 , which encodes a...”
- More
BTH_I0484 PTS IIA-like nitrogen-regulatory protein PtsN from Burkholderia thailandensis E264
41% identity, 60% coverage
- Combining functional and structural genomics to sample the essential Burkholderia structome
Baugh, PloS one 2013 - “...36.4, 86.6 Yes BURPS1710b_0753 Ribose-phosphate pyrophosphokinase (prsA) 3DAH B. pseudomallei BTH_I0474 P11908 47.5, 98.1 Yes BTH_I0484 PTS IIA-like nitrogen-regulatory protein PtsN 3URR B. thailandensis BTH_I0484 Yes BTH_I0732 Ornithine carbamoyltransferase 4F2G B. thailandensis BTH_I0732 P00480 39.8, 96.1 Yes BURPS1710b_1080 Adenylate kinase (adk) 3GMT B. pseudomallei BTH_I0739 P54819...”
AZC_0344 PTS IIA-like nitrogen-regulatory protein from Azorhizobium caulinodans ORS 571
43% identity, 69% coverage
- The genome of the versatile nitrogen fixer Azorhizobium caulinodans ORS571
Lee, BMC genomics 2008 - “...listed in Table 2 (data not shown). Table 2 Nitrogen fixation-related genes Identifier Gene Description AZC_0344 pts N PTS IIA-like nitrogen-regulatory protein AZC_1036 nif X Nitrogenase MoFe cofactor biosynthesis AZC_1037 nif N Nitrogenase MoFe cofactor biosynthesis AZC_1038 nif E Nitrogenase MoFe cofactor biosynthesis AZC_1039 nif K...”
Q5A_022765 PTS IIA-like nitrogen regulatory protein PtsN from Serratia inhibens PRI-2C
32% identity, 95% coverage
PA14_57960 nitrogen regulatory IIA protein from Pseudomonas aeruginosa UCBPP-PA14
Q9HVV4 Nitrogen regulatory protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PA4464 nitrogen regulatory IIA protein from Pseudomonas aeruginosa PAO1
32% identity, 86% coverage
- Loss of the Two-Component System TctD-TctE in Pseudomonas aeruginosa Affects Biofilm Formation and Aminoglycoside Susceptibility in Response to Citric Acid
Taylor, mSphere 2019 - “...2.02 PA14_18360 2.02 PA14_09980 dkgB 2,5-Diketo- d -gluconate reductase B 2.06 PA14_47420 2.13 PA14_55590 2.19 PA14_57960 ptsN Nitrogen regulatory IIA protein 2.21 PA14_00990 2.22 PA14_56980 2.23 PA14_52070 2.24 PA14_10170 fepB Ferrienterobactin-binding periplasmic protein precursor 2.70 a Where no gene name is provided, it is an uncharacterized...”
- Protein interactions in human pathogens revealed through deep learning
Humphreys, Nature microbiology 2024 - “...to WbpG 38 , a hypothesis our interaction finding supports. The PtsHPtsN ( Q9HVV2 - Q9HVV4 ) pair with the highest support by STRING (score=959) failed to generate a positive Co-IP signal (Supplementary Fig. 13 ); PtsH is a histidine-phosphorylatable phosphocarrier protein encoded adjacent to PtsN,...”
- Differential transcription profiling of the phage LUZ19 infection process in different growth media
Brandão, RNA biology 2021 (secret) - The Breadth and Molecular Basis of Hcp-Driven Type VI Secretion System Effector Delivery
Howard, mBio 2021 - “...4.4 2.02 Bifunctional purine biosynthesis protein 57.7 PA2623 Icd 4.2 3.12 Isocitrate dehydrogenase (NADP) 45.6 PA4464 PtsN 4 1.30 Nitrogen regulatory protein 16.7 PA2015 LiuA 4 2.25 Putative isovaleryl-CoA dehydrogenase 42.2 PA4483 GatA 3.9 1.39 Glutamyl-tRNA(Gln) amidotransferase subunit A 51.9 PA3183 Zwf 3.9 2.06 Glucose-6-phosphate 1-dehydrogenase...”
- Overexpression of the Small RNA PA0805.1 in Pseudomonas aeruginosa Modulates the Expression of a Large Set of Genes and Proteins, Resulting in Altered Motility, Cytotoxicity, and Tobramycin Resistance
Coleman, mSystems 2020 - “...PA4315 mvaT Transcriptional regulator mvat, P16 subunit 1.26 2.6E02 PA4396 Two-component response regulator 2.15 4.8E11 PA4464 ptsN Nitrogen-regulatory IIA protein 1.74 1.6E10 PA4493 roxR Regulatory protein 1.26 2.5E02 PA4546 pilS Two-component sensor 1.28 3.1E03 PA4600 nfxB Transcriptional regulator 1.25 4.7E02 PA4601 morA Motility regulator 1.54 1.2E12...”
- Negative control of quorum sensing by RpoN (sigma54) in Pseudomonas aeruginosa PAO1
Heurlier, Journal of bacteriology 2003 - “...in PCR. The assignment of pstN to open reading frame PA4464 is based on sequence comparison with the rpoN gene cluster in E. coli (28). RpoN REPRESSES QUORUM...”
y0157 phosphotransferase system enzyme IIA from Yersinia pestis KIM
34% identity, 80% coverage
VK055_1467 phosphoenolpyruvate--protein phosphotransferase from Klebsiella pneumoniae subsp. pneumoniae
30% identity, 17% coverage
KP1_1993 phosphoenolpyruvate-protein phosphotransferase from Klebsiella pneumoniae NTUH-K2044
KP1_RS09395 phosphoenolpyruvate--protein phosphotransferase from Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
30% identity, 17% coverage
KPK_RS16810 phosphoenolpyruvate--protein phosphotransferase from Klebsiella variicola
30% identity, 17% coverage
- Identification of Pathogenicity-Associated Loci in Klebsiella pneumoniae from Hospitalized Patients
Martin, mSystems 2018 - “..., VK055_1477 , KP1_RS09345 Amino acid ABC transporter substrate-binding protein 4 (10.5) 28 (36.8) 0.005286 KPK_RS16810 , VK055_1467 , KP1_RS09395 e PTS fructose transporter subunit IIA 5 (13.1) 30 (39.4) 0.006209 KPK_RS16815, VK055_1468, KP1_RS09390 PTS fructose transporter subunit IIC 5 (13.1) 30 (39.4) 0.006209 KPK_RS16825, VK055_1470,...”
- “...with bloodstream infection or pneumonia. d Patients with asymptomatic rectal colonization. e Representative gene for KPK_RS16810, -16815, -16825, -16830, -16840, -16845, and -16850. f Representative gene for KPK_RS16820 and -16835. g Representative gene for KP1_RS12820 to -12840. h Representative gene for KP1_RS26720, -26700, -26655, and -26650....”
VPA1424 PTS system, fructose-specific IIABC component from Vibrio parahaemolyticus RIMD 2210633
34% identity, 21% coverage
- Fis Connects Two Sensory Pathways, Quorum Sensing and Surface Sensing, to Control Motility in Vibrio parahaemolyticus
Tague, Frontiers in microbiology 2021 - “...inputs. Default settings were used to obtain putative Fis binding sites. A 130-bp sequence of VPA1424 regulatory region was used as a negative control that contains no Fis binding sites. A 229-bp sequence of gyrA regulatory region was used as a positive control for Fis binding,...”
- “...177-bp fragment of P qrr5 regulatory regions were used as probes. A 130-bp probe of VPA1424 regulatory region was used as a negative control and a 229-bp probe of P gyrA regulatory region was used as a positive control. Analysis of Fis binding to flagellum gene...”
- Quorum Sensing Regulators Are Required for Metabolic Fitness in Vibrio parahaemolyticus
Kalburge, Infection and immunity 2017 - “...binding sites (probability 90%), which included mannose (VPA1424 to VPA1425), arabinose (VPA1673 to VPA1671), and glycogen (VPA1620) transport and/or metabolism...”
- “...region for a mannose transport and metabolism cluster (VPA1424 to VPA1425). The glucose-specific phosphotransferase system (PTS) VPA1667 probe was included as a...”
- Gene expression of Vibrio parahaemolyticus growing in laboratory isolation conditions compared to those common in its natural ocean environment
García, BMC microbiology 2017 - “...207 -7.5 3.5E - 248 Hypothetical protein VP1072 1 142 -7.2 1.1E - 186 Helicase VPA1424 2 492 7.2 0 Fructose transport Overall differential expression To compare the expression of all the differentially expressed genes, they were grouped by gene ontology term using enrichGO from clusterProfiler...”
- Insights into Vibrio parahaemolyticus CHN25 response to artificial gastric fluid stress by transcriptomic analysis
Sun, International journal of molecular sciences 2014 - “...glucose-, fructose-, mannitol-, cellobiose-, ascorbate-, and N -acetylglucosamine-specific enzyme II components. One of these genes, VPA1424 encoding fructose-specific enzyme II ABC subunits, was strongly up-regulated for 27.97-fold in mRNA level, suggesting extremely active transport and utilization of the fructose in the AGF stress. In addition, interestingly,...”
- “...Encoded Protein RIMD2210633 CHN25 LGP cells Phosphotransferase system (PTS) VPA1667 Chn25A_1555 0.0384 Glucose-specific IIBC component VPA1424 Chn25A_1312 27.9696 Fructose-specific IIABC component VPA1422 Chn25A_1310 2.2688 Nitrogen regulatory IIA component VPA1421 Chn25A_1309 2.3425 Fructose-specific IIB component VPA1420 Chn25A_1308 2.8724 Fructose-specific IIABC component VPA0501 Chn25A_1196 2.6299 Mannitol-specific enzyme II...”
A0KI69 protein-N(pi)-phosphohistidine--D-fructose phosphotransferase from Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049)
AHA_1431 pts system, fructose-specific iiabc component from Aeromonas hydrophila subsp. hydrophila ATCC 7966
34% identity, 14% coverage
PP0950 phosphotransferase system, nitrogen regulatory IIA protein from Pseudomonas putida KT2440
32% identity, 86% coverage
ETEC_4216 phosphoenolpyruvate--protein phosphotransferase from Escherichia coli ETEC H10407
30% identity, 17% coverage
FrwA / b3947 putative PTS multiphosphoryl transfer protein PtsA from Escherichia coli K-12 substr. MG1655 (see 4 papers)
b3947 fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component from Escherichia coli str. K-12 substr. MG1655
P32670 Multiphosphoryl transfer protein 2 from Escherichia coli (strain K12)
30% identity, 17% coverage
- The two-component system histidine kinase EnvZ contributes to Avian pathogenic Escherichia coli pathogenicity by regulating biofilm formation and stress responses
Fu, Poultry science 2023 - “...Multidrug/betaine/choline efflux transporter EmrE 2.61 PTS system b2703 srlE PTS system, glucitol/sorbitol-specific IIBC component 2.21 b3947 ptsA Putative PTS multi-phosphoryl transfer protein PtsA 2.20 b2704 srlB PTS system, glucitol/sorbitol-specific IIA component 2.00 b2702 srlA PTS system, glucitol/sorbitol-specific IIC2 component 1.19 b2167 fruA PTS system, fructose-specific IIBC...”
- Chemical and Metabolic Controls on Dihydroxyacetone Metabolism Lead to Suboptimal Growth of Escherichia coli
Peiro, Applied and environmental microbiology 2019 - “...This study dhaKLM b1200, b1199, b1198 BW25113 dhaKLM :: frt This study ptsA :: kan b3947 BW25113 ptsA :: kan 49 ptsA b3947 BW25113 ptsA :: frt This study WT+p131 BW25113 + pSEVA131 This study WT+p234 BW25113 + pSEVA234 This study fsaA +++ b0825 BW25113 +...”
- Escherichia coli toxin/antitoxin pair MqsR/MqsA regulate toxin CspD
Kim, Environmental microbiology 2010 - “...-ribose high-affinity transport system rbsC b3750 3.0 1.0 1.3 d -ribose high-affinity transport system ptsA b3947 1.6 4.0 1.2 PEP-protein phosphotransferase system enzyme I phnM b4095 1.5 4.6 1.6 Phosphonate metabolism phnL b4096 1.8 4.0 1.6 ATP-binding component of phosphonate transport phnH b4100 1.6 4.6 1.4...”
- Stationary-phase quorum-sensing signals affect autoinducer-2 and gene expression in Escherichia coli
Ren, Applied and environmental microbiology 2004 - “...by the fresh E. coli stationary-phase supernatanta Expression ratio b3947 21.4 btuB tnaL b3966 b3707 14.2 10.6 ompC b2215 4.1 tnaA cysA b3708 b2422 3 2.6 fliK...”
- “...supernatanta Gene b no. Expression ratio ptsA b3947 3.9 btuB ompC b3966 b2215 3.0 2.5 a Description PEP-protein phosphotransferase system enzyme...”
- Identification and mapping of self-assembling protein domains encoded by the Escherichia coli K-12 genome by use of lambda repressor fusions
Mariño-Ramírez, Journal of bacteriology 2004 - “...b3724 b0951 b4201 b0467 b0386 b0333 b1304 b1303 b3763 b3947 b0523 b4389 b0892 b1350 b0472 b1349 b2784 b3903 b0415 b0030 b1234 b3422 b1861 b1860 b3591 b4300...”
- A functional update of the Escherichia coli K-12 genome
Serres, Genome biology 2001 - “...the encoded proteins ranging from 1 to 147 amino acids, the latter in PtsA (Bnum b3947, Magnum ec_6103). The other Magnum-identified candidate CDSs include retired Bnums (six Magnums), CDSs located between the boundaries of Bnums (506 Magnums), and CDSs overlapping existing Bnums (730 Magnums). Among the...”
- The Rhizobium etli rpoN locus: DNA sequence analysis and phenotypical characterization of rpoN, ptsN, and ptsA mutants
Michiels, Journal of bacteriology 1998 - “...phosphoenolpyruvate-protein phosphotransferase PtsA (enzyme I; accession no. P32670) from amino acid 111 to 226. The conserved phosphorylation site (H189) is...”
- The umpA gene of Escherichia coli encodes phosphatidylglycerol:prolipoprotein diacylglyceryl transferase (lgt) and regulates thymidylate synthase levels through translational coupling
Gan, Journal of bacteriology 1995 - “...in E. coli has been previously identified (YijH; SWISS-PROT P32670), but the functions of these two E. coli ptsI homologs are not known. Two bases after the...”
TC 4.A.2.1.13 / Q0S1N2 The fructose-specific PTS Enzyme IIABC FruA from Rhodococcus sp. (strain RHA1) (see paper)
40% identity, 18% coverage
Acife_2901 PTS sugar transporter subunit IIA from Acidithiobacillus ferrivorans SS3
38% identity, 66% coverage
- Acidithiobacillus ferrivorans SS3 presents little RNA transcript response related to cold stress during growth at 8 °C suggesting it is a eurypsychrophile
Christel, Extremophiles : life under extreme conditions 2016 - “...Mechanosensitive ion channel protein 16 52 + Acife_2898 PTS sugar transporter subunit 1558 760 NS Acife_2901 PTS sugar transporter 1558 760 NS 5ABC transporter (various) d 28ABC transporter (various) d NS Oxidative stress response Acife_0490 Cobalt transporter 41 137 NS Acife_1063 Cobalt transporter 190 7 Acife_1434...”
- “...a mechanosensitive ion channel protein (Acife_2037), glycine betaine transporter (Acife_1383), and PTS sugar transporters (Acife_2898, Acife_2901) could potentially introduce osmo- and cryoprotectants into the cell (Pflger and Mller 2004 ). Additionally, sucrose synthase and sucrose-phosphate synthase (Acife_1677 and Acife_1678) may synthesize compatible solutes found in cold-grown...”
NO343_01610 fructose-specific PTS transporter subunit EIIC from Mycoplasma capricolum subsp. capricolum
34% identity, 18% coverage
STM4110 General PTS family, enzyme I from Salmonella typhimurium LT2
30% identity, 17% coverage
L21SP2_3409 PTS sugar transporter subunit IIA from Salinispira pacifica
35% identity, 57% coverage
- Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat
Ben, Standards in genomic sciences 2015 - “...(L21SP2_2173 and L21SP2_3294), enzyme I (L21SP2_1343 and L21SP2_3295), and enzyme II (L21SP2_0100, L21SP2_0865, L21SP2_3408, and L21SP2_3409) of a putative PTS transport mechanism were detected. In addition, genes encoding a TRAP-type dicarboxylate transporter (L21SP2_1277 and L21SP2_1278) were present. The assimilation of peptides, which had a stimulating effect...”
VC2531 PTS system, nitrogen regulatory IIA component from Vibrio cholerae O1 biovar eltor str. N16961
29% identity, 83% coverage
CD0206 PTS system, IIa component from Clostridium difficile 630
32% identity, 86% coverage
lmo0631 similar to PTS system, fructose-specific IIA component from Listeria monocytogenes EGD-e
35% identity, 74% coverage
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...lmo0543 PTS sorbitol transporter subunit IIBC lmo0544 4.74 Yes/down lmo0544 PTS sorbitol transporter subunit IIC lmo0631 6.83 Yes/down lmo0631 PTS fructose transporter subunit IIA lmo0632 3.13 Yes/down lmo0632 PTS fructose transporter subunit IIC lmo0633 lmo0633 PTS fructose transporter subunit IIB lmo0738 lmo0738 PTS beta-glucoside transporter subunit...”
- Phosphotransferase system-dependent extracellular growth of listeria monocytogenes is regulated by alternative sigma factors σL and σH
Wang, Applied and environmental microbiology 2014 - “...genes, including lmo0021, lmo0027, lmo0398 to lmo0400, lmo2733, lmo0631, and lmo2665 (13, 14). The growth of L. monocytogenes is positively affected by the...”
- Probing the pan-genome of Listeria monocytogenes: new insights into intraspecific niche expansion and genomic diversification
Deng, BMC genomics 2010 - “...fructose-specific enzyme IIBC component IIIA + 059 lmo0359 Similar to D-fructose-1,6-biphosphate aldolase" IIIA + - lmo0631 Similar to PTS system, fructose-specific IIA component IIIA - - lmo0632 Similar to PTS system, fructose-specific IIC component IIIA + - lmo0633 Similar to PTS system, fructose-specific IIB component IIIA...”
OG1RF_10433 fructose PTS transporter subunit IIA from Enterococcus faecalis OG1RF
30% identity, 82% coverage
TC 4.A.2.1.22 / Q3JZE3 PTS system, fructose-specific, IIA component, component of D-allose/D-ribose transporting Enzyme II complex (Fru2; IIA/IIB/IIC) (Patron et al. 2017). This system is similar to Frz of E. coli (TC#4.A.2.1.9) which is involved in environmental sensing, host adaptation and virulence from Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)
ID870_00995 PTS sugar transporter subunit IIA from Streptococcus agalactiae CJB111
31% identity, 86% coverage
- substrates: D-allose, D-ribose
tcdb comment: The regulatory mechanism has been studied (Patron et al. 2017) - Genomic Analyses Identify Manganese Homeostasis as a Driver of Group B Streptococcal Vaginal Colonization
Burcham, mBio 2022 - “...mannose/fructose/ N -acetylgalactosamine-transporter subunit IIB 7.30 ID870_00990 PTS fructose transporter subunit IIB 9.43 19.55 21.30 ID870_00995 PTS sugar transporter subunit IIA 7.71 6.94 6.60 ID870_01290 PTS glucose transporter subunit IIA 3.29 3.53 3.21 ID870_01665 Transporter substrate-binding domain-containing protein 6.37 6.37 5.75 4.38 ID870_07535 PTS mannose/fructose/sorbose transporter...”
LGG_02647 PTS system, fructose-specific IIABC component from Lactobacillus rhamnosus GG
27% identity, 22% coverage
T256_00805 fructose-specific PTS transporter subunit EIIC from Pediococcus pentosaceus SL4
26% identity, 22% coverage
ESA_03303 hypothetical protein from Enterobacter sakazakii ATCC BAA-894
ESA_03303 fructose-specific PTS transporter subunit EIIC from Cronobacter sakazakii ATCC BAA-894
36% identity, 19% coverage
L21SP2_3408 PTS sugar transporter subunit IIA from Salinispira pacifica
31% identity, 50% coverage
- Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat
Ben, Standards in genomic sciences 2015 - “...phosphocarrier proteins (L21SP2_2173 and L21SP2_3294), enzyme I (L21SP2_1343 and L21SP2_3295), and enzyme II (L21SP2_0100, L21SP2_0865, L21SP2_3408, and L21SP2_3409) of a putative PTS transport mechanism were detected. In addition, genes encoding a TRAP-type dicarboxylate transporter (L21SP2_1277 and L21SP2_1278) were present. The assimilation of peptides, which had a...”
MGA_0508 PTS system fructose-specific transporter subunit EIIABC from Mycoplasma gallisepticum str. R(low)
29% identity, 18% coverage
- Comparative genomic analyses of attenuated strains of Mycoplasma gallisepticum
Szczepanek, Infection and immunity 2010 - “...the fructose/mannose-specific IIABC component gene fruA (MGA_0508) (60) of the phosphoenolpyruvate:fructose phosphotransferase system (PTS) are likely inactive,...”
- “...promoter region Intergenic, VlhA3.09 promoter region MGA_0508, fructose-specific PTS component CRISPR CRISPR Intergenic, MGA_0536 ATPase and MGA_0537 hsdM...”
NR795_17750 PTS IIA-like nitrogen regulatory protein PtsN from Pseudescherichia sp. L3
32% identity, 79% coverage
pRL110376 putative regulator from Rhizobium leguminosarum bv. viciae 3841
35% identity, 77% coverage
c4487 Putative phosphotransferase system (PTS), fructose-specific from Escherichia coli CFT073
28% identity, 96% coverage
TC 4.A.2.1.9 / Q1R4S9 Putative phosphotransferase system (PTS), fructose-specific, component of The FrzABC PTS putative transporter (promotes bacterial fitness under stress conditions and promotes fimbrial (fim) gene expression indirectly (Rouquet et al., 2009). Might transport D-tagatose, D-psicose and/or D-sorbose, or a disaccharide of these (Rouquet et al. 2009); involved in environmental sensing, host adaptation and virulence from Escherichia coli (strain UTI89 / UPEC) (see paper)
28% identity, 96% coverage
- substrates: Sorbose, Tagatose, psicose
KP1_4926 sugar-specific PTS family enzyme IIA component from Klebsiella pneumoniae NTUH-K2044
32% identity, 80% coverage
GM298_07545 PTS IIA-like nitrogen regulatory protein PtsN from Enterobacter sp. HSTU-ASh6
35% identity, 64% coverage
VC1822 PTS system, fructose-specific IIABC component from Vibrio cholerae O1 biovar eltor str. N16961
35% identity, 23% coverage
- A Genome-Wide Screen Reveals that the Vibrio cholerae Phosphoenolpyruvate Phosphotransferase System Modulates Virulence Gene Expression
Wang, Infection and immunity 2015 - “...fpr (VCA0518) C6706 VC1820 C6706 VCA0245 C6706 VC1822 C6706 VC2013 C6706 VC0995 C6706 VCA1045 C6706 VC0910 PtsI(H189A) VC0964(H91D) PtsH(H15A) Lab collection...”
- “...pCVD442 carrying an unmarked, in-frame deletion in VC1822 pCVD442 carrying an unmarked, in-frame deletion in VC2013 pCVD442 carrying an unmarked, in-frame...”
- Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germfree mouse intestine
Houot, Infection and immunity 2010 - “...of each of the following targeted genes (VC0672, VC1820, VC1822, VC1826, VCA1045, VCA0245, and VC1283) was amplified by PCR using the primer sets listed in...”
- “...fragment fragment of of of of of of of VC1820 VC1822 VC1826 VCA1045 VCA0245 VC0672 VC1283 the coding sequence of VC0965 the coding sequence of VC0966 a VC0966...”
- Genes induced late in infection increase fitness of Vibrio cholerae after release into the environment
Schild, Cell host & microbe 2007 - “...hypothetical protein 6.6 ND 4.3 53.9 8.2 12.6 ND VC1822 PTS system, fructose-specific IIABC component ( frwABC ) 20.4 ND 19.9 85.3 4.2 4.3 ND VC1906...”
MPN078 fructose-permease IIBC component from Mycoplasma pneumoniae M129
33% identity, 14% coverage
PtsN / b3204 phosphotransferase system enzyme IIA(Ntr) from Escherichia coli K-12 substr. MG1655 (see 22 papers)
ptsN / AAB60165.1 Enzyme IIANtr from Escherichia coli (see 3 papers)
NP_417671 phosphotransferase system enzyme IIA(Ntr) from Escherichia coli str. K-12 substr. MG1655
b3204 sugar-specific enzyme IIA component of PTS from Escherichia coli str. K-12 substr. MG1655
32% identity, 79% coverage
- Proteome analysis of an Escherichia coli ptsN-null strain under different nitrogen regimes.
Gravina, Journal of proteomics 2018 (PubMed)- GeneRIF: ptsN is required for fitness under minimal medium and for the proper balance between RpoS and sigma 70 activities in such way that, in the absence of ptsN, RpoS-dependent genes are preferentially expressed.
- Growth Inhibition by External Potassium of Escherichia coli Lacking PtsN (EIIANtr) Is Caused by Potassium Limitation Mediated by YcgO.
Sharma, Journal of bacteriology 2016 - GeneRIF: Overproduction of YcgO yields a potassium-sensitive phenotype with similarities to that displayed by the DeltaptsN mutation.
- Reciprocal regulation of the autophosphorylation of enzyme INtr by glutamine and α-ketoglutarate in Escherichia coli.
Lee, Molecular microbiology 2013 - GeneRIF: Glutamine and alpha-ketoglutarate regulate the phosphorylation state of the PTSNtr by direct effects on enzyme INtr autophosphorylation.
- Characteristic phenotypes associated with ptsN-null mutants in Escherichia coli K-12 are absent in strains with functional ilvG.
Reaves, Journal of bacteriology 2011 - GeneRIF: Here the authors show that all of the nutrient utilization phenotypes attributed to ptsN are manifested selectively in strains lacking functional ilvG.
- Potassium mediates Escherichia coli enzyme IIA(Ntr) -dependent regulation of sigma factor selectivity.
Lee, Molecular microbiology 2010 (PubMed)- GeneRIF: PtsN controls sigma(70) and sigma(S) selectivity by regulating the intracellular K(+) level.
- Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIA(Ntr) in Escherichia coli.
Lüttmann, Molecular microbiology 2009 (PubMed)- GeneRIF: Dephosphorylated IIANtr enhances activity of the kdp promoter by direct binding of dephosphorylated IIA(Ntr) to the sensor kinase KdpD.
- The extracytoplasmic stress factor, sigmaE, is required to maintain cell envelope integrity in Escherichia coli.
Hayden, PloS one 2008 - GeneRIF: overexpression of ptsN reduces stress in the cell envelope, thereby promoting survival in the absence of sigma(E).
- Requirements for the phosphorylation of the Escherichia coli EIIANtr protein in vivo.
Zimmer, FEMS microbiology letters 2008 (PubMed)- GeneRIF: EIIANtr is readily phosphorylated at the histidine 73.
- More
- In silico prediction and functional validation of sigma28-regulated genes in Chlamydia and Escherichia coli
Yu, Journal of bacteriology 2006 - “...name b0132 b0763 b1760 yadD modA ynjH b1915 b3204 b3490 b4083 yecF ptsN yhiL yjcS Function Hypothetical protein Molybdate ABC transporter NADPH thioredoxin...”
GJQ69_00895 PTS sugar transporter subunit IIA from Caproicibacterium lactatifermentans
29% identity, 76% coverage
- Revealing the Characteristics of Glucose- and Lactate-Based Chain Elongation for Caproate Production by Caproicibacterium lactatifermentans through Transcriptomic, Bioenergetic, and Regulatory Analyses
Wang, mSystems 2022 - “...and biomass synthesis. The enzyme II (EII) components of sugar phosphotransferase system (PTS)-encoding genes (GJQ69_00890, GJQ69_00895, GJQ69_00900, and GJQ69_01220) and a phosphocarrier protein (HPr)-encoding gene (GJQ69_00905) were significantly upregulated with glucose as carbon source, suggesting that glucose was taken up by PTS rather than ATP-binding cassette...”
- “...is located downstream of a PTS operon which consists of genes encoding PTS enzyme II (GJQ69_00895, GJQ69_00900) and a phosphoryl carrier protein (HPr, GJQ69_00905) ( Fig.9A ). Genes GJQ69_00895 and GJQ69_00900 encode PTS subunits IIA and IIBC, respectively, both of which showed more than 4-fold increased...”
MG_062 PTS system, fructose-specific IIABC component from Mycoplasma genitalium G37
P47308 PTS system fructose-specific EIIABC component from Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
33% identity, 15% coverage
SARI_03955 hypothetical protein from Salmonella enterica subsp. arizonae serovar 62:z4,z23:--
35% identity, 22% coverage
SMU_1183, SMU_RS05445 PTS sugar transporter subunit IIA from Streptococcus mutans UA159
33% identity, 91% coverage
- Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability
Naka, Frontiers in cellular and infection microbiology 2022 - “...Cation-transporting P-type ATPase PacL 1028803 SMU_1563 2.540 mtlA2 PTS system mannitol-specific transporter subunit IIA 1028483 SMU_1183 2.524 SMU_675 PTS system transporter protein EI 1028092 SMU_675 2.503 ptnA PTS system mannose-specific transporter subunit IIAB 1029081 SMU_1877 2.447 opuBa Choline transporter ABC transporter ATP-binding protein 1028412 SMU_1096 2.421...”
- Identification of the antibacterial action mechanism of diterpenoids through transcriptome profiling
Moon, Frontiers in microbiology 2022 - “...0.83 2.76 1.55 1.79 SMU_RS08595 PTS mannose/fructose/sorbose transporter subunit IIC ptnC 1.08 3.16 1.87 2.14 SMU_RS05445 PTS sugar transporter subunit IIA mtlA2 3.10 1.12 2.38 2.39 SMU_RS05455 PTS mannitol transporter subunit IICBA mtlA1 3.34 1.65 1.41 SMU_RS01555 PTS glucitol/sorbitol transporter subunit IIA 0.88 1.68 1.79 1.85...”
- Transcriptional Profiling Reveals the Importance of RcrR in the Regulation of Multiple Sugar Transportation and Biofilm Formation in Streptococcus mutans
Gong, mSystems 2021 - “...Fig.5 ), which were classified by two sugar transporter operons, the mannitol-specific PTS system ( SMU_1183 , SMU_1184c , and SMU_1185 ) and the trehalose-specific PTS system ( SMU_2037 and SMU_2038 ) ( Fig.8A ). The promoter regions ( P SMU_1185 and P SMU_2038 ) were...”
- “...was used to verify whether RcrR could upregulate two PTS operons. The expression levels of SMU_1183 , SMU_1184c , SMU_1185 , SMU_2037 , and SMU_2038 in the rcrR -overexpressing strain were found to be upregulated 2- to 7-fold compared with the wild-type strain by quantitative reverse...”
CDW43_RS15625 PTS sugar transporter subunit IIA from Methylophaga nitratireducenticrescens
29% identity, 91% coverage
TP0755 PTS system, nitrogen regulatory IIA component (ptsN-2) from Treponema pallidum subsp. pallidum str. Nichols
31% identity, 81% coverage
BCAM0545 putative PTS transport system from Burkholderia cenocepacia J2315
27% identity, 60% coverage
1j6tA / P00550 Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
34% identity, 90% coverage
- Ligand: phosphite ion (1j6tA)
PMPD1_0116 PTS mannitol transporter subunit IICBA from Paramixta manurensis
34% identity, 20% coverage
BB0629 PTS system, fructose-specific IIABC component (fruA-2) from Borrelia burgdorferi B31
27% identity, 22% coverage
- BosR and PlzA reciprocally regulate RpoS function to sustain Borrelia burgdorferi in ticks and mammals
Grassmann, The Journal of clinical investigation 2023 - “...bb0166 / malQ , bb0367 , bb0557 / ptsH-2 , bb0559 / crr , and bb0629 / fruA-2 ) encode components of the phosphoenolpyruvate-dependent sugar phosphotransferase system the spirochetes central pathway for carbohydrate transport ( 42 , 43 ) and could be involved in uptake of...”
- N-acetylmannosamine (ManNAc) supports the growth of Borrelia burgdorferi in the absence of N-acetylglucosamine (GlcNAc)
Schneider, FEMS microbiology letters 2018 (PubMed)- “...encoding a subunit of the chitobiose transporter, bb0629 (fruA-2) encoding a putative carbohydrate transporter and bb0644 (nanE) encoding a putative...”
- “...in this study. Symbol bb0147 flaB bb0374 pdeB bb0628 bb0629 fruA-2 bb0644 nanE bba30 bbb04 bbd22 bbg28 chbC Primer No. NC374 NC375 NC510 NC511 NC506 NC507 NC508...”
- Global Tn-seq analysis of carbohydrate utilization and vertebrate infectivity of Borrelia burgdorferi
Troy, Molecular microbiology 2016 - “...of ten genes annotated to encode EII PTS transporter subunits ( bb0116, bb0367, bb0408, bb0559, bb0629, bb0645, bbb04, bbb05, bbb06, bbb29 ) suggests that this method of importing carbohydrates plays a significant role in the enzootic cycle. Transposon mutants with insertions in eight of these genes...”
- “...burgdorferi genome predicted to encode transporters in the PTS Fructose-Mannitol (Fru) family: bb0408 (fruA1) and bb0629 (fruA2) . Both of these genes are predicted to encode all three EII domains of a PTS transporter. The PTS Fru family includes a number of fructose, mannose and mannitol...”
- Phosphoenolpyruvate Phosphotransferase System Components Modulate Gene Transcription and Virulence of Borrelia burgdorferi
Khajanchi, Infection and immunity 2015 - “...T08P02E04 T04TC008 T05TC295 BB0723 BB0723 BB0645 BB0645 BB0408 BB0629 BB0629 BB0629 BB0629 BB0116 BBB29 BBB05 cyaB cyaB ptsG ptsG fruA1 fruA2 fruA2 fruA2 fruA2...”
- Cyclic di-GMP modulates gene expression in Lyme disease spirochetes at the tick-mammal interface to promote spirochete survival during the blood meal and tick-to-mammal transmission
Caimano, Infection and immunity 2015 - “...(bb0116) glpF (bb0240) glpK (bb0241) glpD (bb0243) bb0323 bb0629 bosR (bb0647) bb0680 rpoS (bb0771) spoVG (bb0785) arcA (bb0841) bba07 ospA (bba15) dbpA (bba24)...”
- “...(PTS) sugar transporter (EII) components for mannose (bb0629), chitobiose (bbb04 to bbb06), and GlcNAc (bbb29 [malX-2]) Infection and Immunity August 2015...”
- Stage-specific global alterations in the transcriptomes of Lyme disease spirochetes during tick feeding and following mammalian host adaptation
Iyer, Molecular microbiology 2015 - “...Presumably, GlcNAc can also be transported into the cell via these EII components. BB0408 and BB0629 initially were annotated as fructose-specific ( Fraser et al. , 1997 ), but fructose does not support B. burgdorferi growth in vitro ( von Lackum and Stevenson, 2005 ; Gherardini...”
- “...9B ), a finding confirmed by qRT-PCR ( Table 1 ). The putative mannose-specific EIIABC (BB0629) also is induced in feeding ticks (23-fold [P=0.0003] in fed larvae; 10-fold [P=.01] in fed nymphs) relative to DMCs. Glucose should be abundantly available in a mammalian environment and during...”
- Transposon mutagenesis as an approach to improved understanding of Borrelia pathogenesis and biology
Lin, Frontiers in cellular and infection microbiology 2014 - “...al., 2012 ) FlgI BB0772 Non-infectious PHOSPHOTRANSFERASE SYSTEM ptsG BB0645 Non-infectious FruA1 BB0408 Non-infectious FruA2 BB0629 Variable infectivity MalX1 BB0116 Intermediate infectivity MalX2 BBB29 Intermediate infectivity ChbB BBB06 Non-infectious ABC TRANSPORT PROTEINS, BORRELIA EFFLUX SYSTEM (Bes), AND OTHER TRANSPORT SYSTEMS ProX BB0144 Intermediate infectivity MglA BB0318...”
- Analysis of an ordered, comprehensive STM mutant library in infectious Borrelia burgdorferi: insights into the genes required for mouse infectivity
Lin, PloS one 2012 - “...bb0408 , fructose-specific IIABC component). Inconsistent results were obtained with two mutants in fruA-2 ( bb0629 ), precluding interpretation in the absence of additional information. In terms of other transporter systems (Fig. 8D in File S1), mutation of the genes encoding lactose permease ( lctP ),...”
- More
Sde_3180 response regulator receiver sensor signal transduction histidine kinase from Saccharophagus degradans 2-40
32% identity, 63% coverage
BB0447 Na+/H+ antiporter (napA) from Borrelia burgdorferi B31
30% identity, 15% coverage
- Weak Organic Acids Decrease Borrelia burgdorferi Cytoplasmic pH, Eliciting an Acid Stress Response and Impacting RpoN- and RpoS-Dependent Gene Expression
Dulebohn, Frontiers in microbiology 2017 - “...a favorable pH i . These include: (i) putative coupled proton exchange with cations (BB0637BB0638, BB0447, BB0724BB0725, BB0380) and Na + /H + antiporters (BB0637BB0638); (ii) transporting neutralizing buffering molecules (polyamines: BB0639BB0640); (iii) amino acid deaminases (BB0841BB0843); (iv) and proton export via a V o V...”
- Two Different Virulence-Related Regulatory Pathways in Borrelia burgdorferi Are Directly Affected by Osmotic Fluxes in the Blood Meal of Feeding Ixodes Ticks
Bontemps-Gallo, PLoS pathogens 2016 - “...2+ transport system ( bb0164 ), the three Na + /H + antiporter systems ( bb0447 and nhaC-1 , nhaC-2 ) and the Mg 2+ uptake system ( mgtE , bb0380 ). The expression of both nhaC-1 and nhaC-2 increased 10-fold at 250 mOsm osmolarity, suggesting...”
- “...during nymph feeding ( Fig 8B ). The three Na + /H + antiporters ( bb0447 , nhaC-1 , nhaC-2 ) were induced during the feeding, increasing 8.6-fold, 2.1-fold and 2.7-fold respectively ( Fig 8B ). The expression of bb0447 and nhaC-1 in replete ticks returned...”
- Stage-specific global alterations in the transcriptomes of Lyme disease spirochetes during tick feeding and following mammalian host adaptation
Iyer, Molecular microbiology 2015 - “...cyclic-di-GMP phosphodiesterase, PdeA bb0384 basic membrane protein C, BmpC + + bb0400 hypothetical protein + bb0447 Na+/H+ antiporter, NapA + bb0467 Multicopper polyphenol oxidase (laccase), putative + + bb0548 DNA polymerase I, PolA + + bb0563 hypothetical protein + + bb0565 purine-binding chemotaxis protein, CheW-2 +...”
- Analysis of the RpoS regulon in Borrelia burgdorferi in response to mammalian host signals provides insight into RpoS function during the enzootic cycle
Caimano, Molecular microbiology 2007 - “...bbq65 bba01 bbg11 bbg30 bbb09 bbl29 bb0400 bbg26 bbi43 bb0447 bb0548 bbq58 bb0116 Descriptiona Gene ID Table 1. cont. lp56 lp54 lp28-2 lp28-2 cp26 cp32-8...”
- “...contrast, only a handful of borrelial genes (bb0116, bb0447, bb0548, bb0728, bb0812 and bba34) with predicted roles in spirochete metabolism and/or physiology...”
- Profiling of temperature-induced changes in Borrelia burgdorferi gene expression by using whole genome arrays
Ojaimi, Infection and immunity 2003 - “...product (homologous gene) Paralogous family Fold induction BBS12 BB0447 BBN25 BB0218 BBO25 BB0237 BBH02 BBQ03 BB0377 BBA04 BBK32 BBL23 BB0751 BB0017 BBQ28 BBS14...”
ECs4475 mannitol-specific PTS system enzyme IIABC components from Escherichia coli O157:H7 str. Sakai
34% identity, 22% coverage
SF3633 mannitol-specific PTS system enzyme IIABC components from Shigella flexneri 2a str. 301
S4135 PTS system, mannitol-specific enzyme IIABC components from Shigella flexneri 2a str. 2457T
SF5M90T_3850 PTS mannitol transporter subunit IICBA from Shigella flexneri 5a str. M90T
34% identity, 22% coverage
- Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery
Yang, Nucleic acids research 2005 - “...permease II, A component SDY3143 SBO3055 SSO3086 mtlA PTS system, mannitol permease II, ABC components SF3633 S4135 SBO3597 SSO3809 mtlD Mannitol-1-phosphate dehydrogenase SF3634 S4134 SBO3598 SSO3808 d -sorbitol srlA PTS system, glucitol/sorbitol-specific II, C component SDY2898 SF2725 a S2916 a SBO2816 SSO2846 srlE PTS system, glucitol/sorbitol-specific...”
- Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery
Yang, Nucleic acids research 2005 - “...II, A component SDY3143 SBO3055 SSO3086 mtlA PTS system, mannitol permease II, ABC components SF3633 S4135 SBO3597 SSO3809 mtlD Mannitol-1-phosphate dehydrogenase SF3634 S4134 SBO3598 SSO3808 d -sorbitol srlA PTS system, glucitol/sorbitol-specific II, C component SDY2898 SF2725 a S2916 a SBO2816 SSO2846 srlE PTS system, glucitol/sorbitol-specific II,...”
- RNA-seq analysis of the influence of anaerobiosis and FNR on Shigella flexneri
Vergara-Irigaray, BMC genomics 2014 - “...1.74 SF5M90T_2897 fba fructose-bisphosphate aldolase, class II 1.56 SF5M90T_2404 ptsH PTS system protein HPr 1.56 SF5M90T_3850 mtlA PTS system, mannitol-specific enzyme IIABC components 1.52 SF5M90T_1640 ydhC putative transport protein 1.51 0.69 SF5M90T_2359 beta-fructosidase 1.49 0.72 SF5M90T_3496 tpiA triosephosphate isomerase 1.45 SF5M90T_2405 ptsI PEP-protein phosphotransferase system enzyme...”
Z_RS23610 PTS mannitol transporter subunit IICBA from Escherichia coli O157:H7 str. EDL933
34% identity, 22% coverage
- Transcriptomic Analysis of Viable but Non-Culturable Escherichia coli O157:H7 Formation Induced by Low Temperature
Zhong, Microorganisms 2019 - “...kdg T, brn Q IT, OAT, SSTTA, ITTA 1 Z_RS26780, Z_RS26335, Z_RS25895, Z_RS25370, Z_RS24525, Z_RS24370, Z_RS23610, Z_RS23465, Z_RS22905, Z_RS21350, Z_RS21150, Z_RS21140, Z_RS21025, Z_RS19885, Z_RS18625, Z_RS16005, Z_RS12610, Z_RS12490, Z_RS09475, Z_RS09120, Z_RS04060, Z_RS03890, Z_RS02910, Z_RS02490, Z_RS01960, sec F, pst S, pst A, nep I, lam B, gld A,...”
b3599 Mannitol phosphotransferase system, IIA/IIB/IIC components (EC 2.7.1.197) from Escherichia coli BW25113
MtlC / b3599 mannitol-specific PTS enzyme IICBA component (EC 2.7.1.197; EC 2.7.1.198) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
mtlA / P00550 mannitol-specific PTS enzyme IICBA component (EC 2.7.1.197; EC 2.7.1.198) from Escherichia coli (strain K12) (see 24 papers)
PTM3C_ECOLI / P00550 PTS system mannitol-specific EIICBA component; EIICBA-Mtl; EII-Mtl; EC 2.7.1.197 from Escherichia coli (strain K12) (see 6 papers)
P00550 protein-Npi-phosphohistidine-D-mannitol phosphotransferase (EC 2.7.1.197) from Escherichia coli (see 7 papers)
TC 4.A.2.1.2 / P00550 Mannitol porter (MtlA) (mannitol-1-P forming), the mannitol IICBA complex from Escherichia coli (see 9 papers)
mtlA / GB|AAC76623.1 PTS system mannitol-specific EIICBA component; EC 2.7.1.-; EC 2.7.1.69 from Escherichia coli K12 (see 9 papers)
NP_418056 mannitol-specific PTS enzyme IICBA component from Escherichia coli str. K-12 substr. MG1655
b3599 fused mannitol-specific PTS enzymes: IIA components/IIB components/IIC components from Escherichia coli str. K-12 substr. MG1655
W8U272 PTS system mannitol-specific EIICBA component from Escherichia coli
ETEC_3841 PTS mannitol transporter subunit IICBA from Escherichia coli ETEC H10407
34% identity, 22% coverage
- mutant phenotype: Specific phenotype: utilization of D-Mannitol
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in D-mannitol transport (PubMed:2123863, PubMed:368051, PubMed:6427236). Also able to use D-mannonic acid (PubMed:6427236).
catalytic activity: D-mannitol(out) + N(pros)-phospho-L-histidyl-[protein] = D- mannitol 1-phosphate(in) + L-histidyl-[protein] (RHEA:33363)
subunit: Homodimer. - substrates: mannitol
tcdb comment: The enzyme-transporter has been alterred genetically, sequenced, purified, reconstituted and characterized (Jacobson et al. 1983, Leonard and Saier 1983, Lee and Saier 1983, Manayan et al. 1988). Intramolecular phosphoryl transfer between the A and B domains of IIMtl is rate-limited by chemistry and not by the rate of formation or dissociation of a stereospecific complex in which the active sites are optimally apposed (Suh et al. 2007). Substrates, in addition to D-mannitol, include D-glucitol (D-sorbitol), D-2-amino-2-deoxymannitol, D-2-deoxymannitol and D-arabitol (D-arabinitol) (Jacobson et al. 1983) - A genomic reappraisal of symbiotic function in the aphid/Buchnera symbiosis: reduced transporter sets and variable membrane organisations
Charles, PloS one 2011 - “...[I] Multimeric complex (EIIA/CB) 0 (c)11 (i) Crr:BU063PtsG:BU356 Crr :BUSg_060PtsG:BUSg_344 Crr:BBp_059PtsG:BBp_326 -- 4 PTS-FM 4.A.2.1.2 P00550 - mannitol [I] Homo-dimer (EIICBA) 8 (i) MtlA:BU572 MtlA:BUSg_552 MtlA:BBp_517 - 8 PTS-EI 8.A.7.1.1 P08839 - glucose/mannitol PTS energy coupling Prot Homodimer 0 (c) PtsI:BU064 PtsI:BUsg_061 PtsI:BBp_060 - 8 PTS-HPr...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...methionine sulfoxide reductase 4.99/23,183.84 MtlA P00550 PTS system mannitol-specific EIICBA component 6.05/67,972.26 MtnN P0AF12...”
- A genomic sample sequence of the entomopathogenic bacterium Photorhabdus luminescens W14: potential implications for virulence
Ffrench-Constant, Applied and environmental microbiology 2000 - “...E. coli E. coli Enterobacter aerogenes H. influenzae E. coli AE001166 P00550 U65015 P31448 P04983 P04984 P18812 P45247 P77565 1 (0) 1 (0) 1 (0) 3 (0) 1 (0) 2...”
- Intramolecular domain-domain association/dissociation and phosphoryl transfer in the mannitol transporter of Escherichia coli are not coupled.
Suh, Proceedings of the National Academy of Sciences of the United States of America 2007 - GeneRIF: Intramolecular phosphoryl transfer between the A and B domains of II(Mtl) is rate-limited by chemistry and not by the rate of formation or dissociation of a stereospecific complex in which the active sites are optimally apposed.
- The two-component system histidine kinase EnvZ contributes to Avian pathogenic Escherichia coli pathogenicity by regulating biofilm formation and stress responses
Fu, Poultry science 2023 - “...fruA PTS system, fructose-specific IIBC component 1.93 b1737 celB PTS system, cellobiose-specific IIC component 1.59 b3599 mtlA Fused mannitol-specific PTS enzymes: IIA components/IIB components/IIC components 1.61 b1738 celA PTS system, cellobiose-specific IIB component; PEP-dependent phosphotransferase enzyme IV 1.65 In total, 269 DEGs were annotated in the...”
- Characterization and potential mechanisms of highly antibiotic tolerant VBNC Escherichia coli induced by low level chlorination
Ye, Scientific reports 2020 - “...toxic-protein b4687 shoB toxic membrane protein toxic-protein b2975 glcA glycolate transporter transporter 57 , 58 b3599 mtlA mannitol-specific PTS enzyme: IIA, IIB and IIC components transporter b1743 spy periplasmic ATP-independent protein refolding chaperone, stress-induced stress-induced protein 59 b0221 fadE acyl coenzyme A dehydrogenase stress-induced protein 60...”
- Complete Genome Sequence of ER2796, a DNA Methyltransferase-Deficient Strain of Escherichia coli K-12
Anton, PloS one 2015 - “...37023093703085 between 37294513729452 100330; adds 1 aa extension (ER2796_3671) mtlA2 (Fs) mtlA2 2 frameshift mtlA (b3599) between 37446963744697 37710633771064 (GG) 254637 ER2796_3708 (aa 1253 + 60 aa), and ER2796_3709 (from internal start at aa 306) rph WT e +1 frameshift rph (b3643) 3787535 (C) between 38139023813903...”
- Evolutionary comparisons suggest many novel cAMP response protein binding sites in Escherichia coli
Brown, Proceedings of the National Academy of Sciences of the United States of America 2004 - “...(b1904) yjcB (b4060); yjcC (b4061) nupG (b2964) mtlA (b3599); yibI (b3598) b1458 (b1458) tnaL (b3707) qseA (b3243); yhcR (b3242) yeaA (b1778); b1777 (b1777);...”
- Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis
Løbner-Olesen, Proceedings of the National Academy of Sciences of the United States of America 2003 - “...b4058 b0779 b4044 b4393 b1061 b0243 b1530 b3748 b1037 b2133 b3599 b3702 1.8 (2.1) 1.9 (3.0) 1.3 (0.9) 1.8 (3.0) 1.3 (2.8) 1.3 (2.3) 1.0 (1.3) 1.2 (0.9) 1.2...”
- Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field
Liu, Frontiers in microbiology 2019 - “...41.6 30 1.7653 0.0156 V6FUE1 Pimeloyl-[acyl-carrier protein] methyl ester esterase, GN=bioH 30.1 4 1.7541 0.0492 W8U272 Fused mannitol-specific PTS enzymes: IIA components/IIB components/IIC components, GN=mtlA 36.1 14 1.7486 0.0103 A0A1 3KKE8 Transketolase, GN=EATG_02582 37.5 1 1.7098 0.0264 W8ZZB2 Regulator of ribonuclease activity A, GN=menG 50.3 4...”
- The molecular basis for control of ETEC enterotoxin expression in response to environment and host
Haycocks, PLoS pathogens 2015 - “...) 4153055 TGTGA TCTGAA TCACA ETEC_3840 yibI TGTGA TCTACAG CA TG 4153191 TGTGA TTGATA TCACA ETEC_3841 mtlA TGTGA TGAACG TCAC G 4158433 n.d. ETEC_3846 lldP 4196869 TG CA A TCGATA TCACA ETEC_3886 dinD 4251326 CT T ACTCCTGC TCACA ETEC_3938 ETEC specific 4266125 G GTGA TGGCATC C...”
XOO1282 nitrogen regulatory IIA protein from Xanthomonas oryzae pv. oryzae KACC10331
31% identity, 66% coverage
- Combined Analysis of the Time-Resolved Transcriptome and Proteome of Plant Pathogen Xanthomonas oryzae pv. oryzae
Kim, Frontiers in microbiology 2021 - “...al., 2013 ) such as GlnB ( Xoo0213 ), GlnB ( Xoo4487 ), PtsN ( Xoo1282 ), and DraG ( Xoo1743 ) were upregulated at 560 min and PtsN ( Xoo1282 ) was slightly upregulated at 30 min ( Supplementary Figure 11 ). In the time-supervised...”
- “...nitrogen metabolism-related genes. Genes like GlnB ( Xoo0213 ), GlnB ( Xoo4487 ), PtsN ( Xoo1282 ), and DraG ( Xoo1743 ) were upregulated at 560 min in transcriptome data. In proteome data, PtsN ( Xoo1282 ) was slightly upregulated at 30 min. Click here for...”
SPAB_04578 hypothetical protein from Salmonella enterica subsp. enterica serovar Paratyphi B str. SPB7
STM3685 PTS family, mannitol-specific enzyme IIABC components from Salmonella typhimurium LT2
SPA3536 mannitol-specific enzyme II of phosphotransferase system from Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150
33% identity, 22% coverage
- Core Proteomic Analysis of Unique Metabolic Pathways of Salmonella enterica for the Identification of Potential Drug Targets
Uddin, PloS one 2016 - “...49 senb NC_022241 2lp4_A 227 84.1 161505779 SARI_03955 50 ses NC_010067 1j6t_A 146 97.9 161616759 SPAB_04578 50 spq NC_010102 1j6t_A 146 97.9 161504756 SARI_02879 65 ses NC_010067 4kqr_B 549 45.7 161612466 SPAB_00156 65 spq NC_010102 4kqr_B 549 45.7 161503040 SARI_01104 67 ses NC_010067 4kqr_B 539 43.0...”
- Global transcriptional analysis of dehydrated Salmonella enterica serovar Typhimurium
Gruzdev, Applied and environmental microbiology 2012 - “...(STM3535) mglB (STM2190) pps (STM1349) mtlA (STM3685) Glycogen synthase Galactose transport protein Phosphoenolpyruvate synthase Mannitol-specific enzyme IIABC...”
- Genome wide evolutionary analyses reveal serotype specific patterns of positive selection in selected Salmonella serotypes
Soyer, BMC evolutionary biology 2009 - “...and binding proteins" STM1679 oppA Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein 1605 Ch# (0.0121) - STM3685 mtlA PTS system, mannitol-specific IIC component subfamily, putative PTS system IIA component, putative 1914 TO (0.0035) - STM3258 - 462 Ty# (0.0157) 124, 139, 143, 144, 147 STM3626 oppF Oligopeptide...”
- Analysis of Salmonella enterica serotype paratyphi A gene expression in the blood of bacteremic patients in Bangladesh
Sheikh, PLoS neglected tropical diseases 2010 - “...0.0274 2.39 0.0294 SPA2035 kdpA Potassium-transporting ATPase A chain 6.80 0.0032 5.58 0.0268 2.95 0.0499 SPA3536 mtlA Mannitol-specific enzyme II of phosphotransferase system 6.24 0.0035 1.11 0.0285 4.39 0.0272 Unclassified SPA0411 eutC Ethanolamine ammonia-lyase light chain 8.68 0.0217 3.42 0.0295 1.50 0.0450 Unknown function SPA0409 eutA...”
YPO4068 PTS system, mannitol-specific IIABC component from Yersinia pestis CO92
32% identity, 21% coverage
- Investigation of and Response to 2 Plague Cases, Yosemite National Park, California, USA, 2015
Danforth, Emerging infectious diseases 2016 - “...YPO3481 3886839 SNP C T YPO3490 3898668 SNP T C YPO3828 4296699 8-bp INDEL Insertion YPO4068 4587603 SNP G T *INDEL,insertion/deletion; MLST,multilocus sequence typing; ORF,open reading frame; SNP,single-nucleotide polymorphism; VNTR,variable number of tandem repeats; , none.PacBio (Pacific Biosciences, Menlo Park, CA, USA) sequencing of patient isolates...”
EAE_06200 PTS mannitol transporter subunit IICBA from Klebsiella aerogenes KCTC 2190
33% identity, 21% coverage
- Transcriptional effects of melatonin on the gut commensal bacterium Klebsiella aerogenes
Graniczkowska, Genomics 2022 - “...nM melatonin as compared to control cells Gene ID Product Description Growth stage fold change EAE_06200 MtlA mannitol transporter IIBC component exponential 1.835 EAE_18025 MalX maltose transporter exponential 1.788 EAE_03850 DhaK dihydroxyacetone kinase subunit exponential 2.633 EAE_03855 DhaL dihydroxyacetone kinase subunit exponential 2.292 EAE_10515 CelB lactose/cellobiose-specific...”
EF0412 PTS system, mannitol-specific IIA component from Enterococcus faecalis V583
OG1RF_10297 PTS sugar transporter subunit IIA from Enterococcus faecalis OG1RF
32% identity, 97% coverage
- Genomewide Profiling of the Enterococcus faecalis Transcriptional Response to Teixobactin Reveals CroRS as an Essential Regulator of Antimicrobial Tolerance
Darnell, mSphere 2019 - “...2555 11.6 PTS mannitol transporter subunit IICB EF3139 138 10.9 PTS sugar transporter subunit IIC EF0412 2554 10.2 PTS mannitol transporter subunit IIA EF3141 136 9.9 2-Hydroxyacid dehydrogenase EF2965 2466 9.9 PTS sugar transporter subunit IIB EF3142 135 9.7 6-Phosphogluconate dehydrogenase EF0413 2553 9.7 Mannitol-1-phosphate 5-dehydrogenase...”
- Functional studies of E. faecalis RNase J2 and its role in virulence and fitness
Gao, PloS one 2017 - “...protein -11.48 0.013 ef3178 peptidase, M20/M25/M40 family -3.08 0.017 ef0411 PTS system component 3.70 0.074 ef0412 PTS system component 5.56 0.081 ef0413 mannitol-1-phosphate 5-dehydrogenase 3.85 0.155 ef0470 ribonucleoside-diphosphate reductase 2 2.44 0.106 ef0471 ribonucleoside-diphosphate reductase 2 2.94 0.011 ef0472 nrdI protein 2.86 0.017 ef0473 ribonucleoside-diphosphate reductase...”
- Comparison of OG1RF and an isogenic fsrB deletion mutant by transcriptional analysis: the Fsr system of Enterococcus faecalis is more than the activator of gelatinase and serine protease
Bourgogne, Journal of bacteriology 2006 - “...Late log arcA argF-1 arcC-1 PTS system EF0411 EF0412 EF0413 EF0955 EF0956 EF0957 EF0958 EF3210 EF3211 EF3212 EF3213 Mannitol-specific IIBC components mltF mtlD...”
- Parallel Genomics Uncover Novel Enterococcal-Bacteriophage Interactions
Chatterjee, mBio 2020 - “...that the levels of Fsr-activated genes involved in the phosphotransferase sugar transport system (OG1RF_10296 and OG1RF_10297) are reduced, whereas negatively regulated genes in the fsr regulon, such as eutB , eutC , and eutH genes involved in ethanolamine utilization, are derepressed during VPE25 infection ( Fig.7B...”
XC_1309 nitrogen regulatory IIA protein from Xanthomonas campestris pv. campestris str. 8004
32% identity, 66% coverage
- HprKXcc is a serine kinase that regulates virulence in the Gram-negative phytopathogen Xanthomonas campestris
Li, Environmental microbiology 2019 - “...two EIIAlike proteins EIIA Man (PtsN Man , XC_1306) and EIIA Ntr (PtsN Ntr , XC_1309). Interestingly, the cluster also encodes an PtsH (HPr) (Ser) kinase homologue (XC_1308, herein named HprK Xcc ), but no the CcpA homologue. To gain insight into the role of these...”
- “...( XC_1304 ), ptsH ( XC_1305 ), ptsN Man ( XC1306 ), ptsN Ntr ( XC_1309 ) and hprK Xcc ( XC_1308 ) were constructed by using the suicide vector pK18mob sacB (see Methods) and the respective strains were designated ptsI, ptsH, ptsN Man , ptsN...”
VV2_1357 Phosphotransferase system mannitol from Vibrio vulnificus CMCP6
29% identity, 77% coverage
B1ZQW4 DAC domain-containing protein from Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1)
31% identity, 26% coverage
HMPREF0010_00382 phosphoenolpyruvate--protein phosphotransferase from Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841
28% identity, 14% coverage
- Delineating the Plausible Molecular Vaccine Candidates and Drug Targets of Multidrug-Resistant Acinetobacter baumannii
Mujawar, Frontiers in cellular and infection microbiology 2019 - “...EC measures, while HMPREF0010_01789 from EC and three candidates from BC, namely, HMPREF0010_03553, HMPREF0010_01114, and HMPREF0010_00382, were unique. It is important to note that sorting of the whole genome proteins as per the four centrality measures yielded 2, 0, 14, and 15, respectively, of which 0,...”
- Constraint-based modeling identifies new putative targets to fight colistin-resistant A. baumannii infections
Presta, Scientific reports 2017 - “...yes no yes no no no HMPREF0010_03273 glucose-6-phosphate isomerase yes no yes no no no HMPREF0010_00382 phosphotransferase system fructose-specific EI/HPr/EIIA component yes no yes no no no HMPREF0010_03275 UTP-glucose-1-phosphate uridylyltransferase yes no yes no no no HMPREF0010_01305 alphaalpha-trehalose-phosphate synthase (UDP-forming) yes no yes no no no...”
VPA0298 PTS system, fructose-specific IIA component from Vibrio parahaemolyticus RIMD 2210633
26% identity, 94% coverage
- Insights into Vibrio parahaemolyticus CHN25 response to artificial gastric fluid stress by transcriptomic analysis
Sun, International journal of molecular sciences 2014 - “...II component VPA0500 Chn25A_1197 2.6362 Mannitol-specific enzyme II component VPA0297 Chn25A_0302 8.029 fructose-specific IIBC component VPA0298 Chn25A_0303 2.8846 Fructose-specific IIA component VPA0231 Chn25A_0232 2.0079 Phosphotransferase enzyme II, A component VPA0230 Chn25A_0231 5.501 Putative sugar phosphotransferase component II B VP2637 Chn25_2566 2.2518 Cellobiose-specific IIB component VP2636 Chn25_2565...”
- “...PTS VP2674 Chn25_2602 2.2237 Phosphocarrier protein NPr VPA0297 Chn25A_0302 0.4786 PTS system, fructose-specific IIBC component VPA0298 Chn25A_0303 0.4004 PTS system, fructose-specific IIA component VPA1424 Chn25A_1312 0.3946 PTS system, fructose-specific IIABC component Quaternary ammonium group transport VPA1111 Chn25A_0571 0.453 Putative glycine betaine-binding ABC transporter Aromatic compound catabolic...”
VSAL_II0090 PTS system, fructose-specific IIA/FPR component from Vibrio salmonicida LFI1238
VSAL_II0090 fused PTS fructose transporter subunit IIA/HPr protein from Aliivibrio salmonicida LFI1238
30% identity, 34% coverage
CIRMBP1228_02731 PTS sugar transporter subunit IIA from Enterococcus cecorum
29% identity, 85% coverage
BMMGA3_RS01075 PTS sugar transporter subunit IIA from Bacillus methanolicus MGA3
33% identity, 80% coverage
- Characterization of D-Arabitol as Newly Discovered Carbon Source of Bacillus methanolicus
López, Frontiers in microbiology 2019 - “...PTS system mannitol-specific EIICB component b -4.22 BMMGA3_RS01070 mtlR b Transcriptional regulator MtlR b -4.62 BMMGA3_RS01075 mtlF b Mannitol-specific phosphotransferase enzyme IIA component b -3.62 BMMGA3_RS01080 mtlD b Mannitol-1-phosphate 5-dehydrogenase b -3.87 BMMGA3_RS07325 Transcriptional antiterminator BglG 2.99 BMMGA3_RS07330 atlA c IIA arabitol PTS component c 3.06...”
D8MLM5 PTS system mannitol-specific EIICBA component from Erwinia billingiae (strain Eb661)
30% identity, 21% coverage
TC 4.A.2.1.19 / M7QAG8 PTS system tagatose-specific IIA-TPr component, component of The tagatose-specific PTS transporter/kinase, TagIIA-TPr/TagIIB'BC (tagatose-1-P forming) from Klebsiella pneumoniae ATCC BAA-2146
32% identity, 50% coverage
- substrates: Tagatose
tcdb comment: TagIIA-TPr is a fusion of a IIA domain fused N-terminal to an HPr domain. TagIIB'BC has and inactive IIB' domain fused N-terminal to the active C-terminal IIBC domains. This arrangement resembles that for the E. coli fructose Enzyme II complex
VC1820 PTS system, fructose-specific IIA component from Vibrio cholerae O1 biovar eltor str. N16961
27% identity, 77% coverage
- A mannose-sensing AraC-type transcriptional activator regulates cell-cell aggregation of Vibrio cholerae
Lee, NPJ biofilms and microbiomes 2022 - “...differentially expressed between the two strains (Supplementary Table 1 ). Interestingly, the expression of all VC1820 to VC1827 genes (Supplementary Fig. 8a ) was significantly increased in the WT cells grown on mannose or fructose, but not in the manR mutant cells. Genes VC1820 to VC1824...”
- “...EIIB, and nitrogen regulatory EIIA component, respectively. To confirm that ManR regulates the transcription of VC1820 to VC1824 genes, qRT-PCR and EMSA were performed. As expected, the expression of these genes increased in the WT strain in the presence of mannose and fructose, however, not in...”
- Identification of Small Molecule Inhibitors of the Pathogen Box against Vibrio cholerae
Kim, Microbiology spectrum 2021 - “...downregulated group was genes involved in carbon metabolism/transport. A few of the most downregulated genes, vc1820 , vc1826 , and vc1821 , are involved in the uptake of fructose. Iron homeostasis/transport showed the most upregulated number of genes along with ribosomal proteins ( Fig.2A ). These...”
- Population Analysis of Vibrio cholerae in Aquatic Reservoirs Reveals a Novel Sister Species (Vibrio paracholerae sp. nov.) with a History of Association with Humans
Islam, Applied and environmental microbiology 2021 - “...capable of utilizing this sugar ( 3 ). The gene cluster encompassing manP to manA (VC1820 to VC1827 in the N16961 genome AE003852.1), including the well-known mannose-6 phosphate isomerase ( manA ) gene required for this process ( 29 ), was present in all the tested...”
- A Genome-Wide Screen Reveals that the Vibrio cholerae Phosphoenolpyruvate Phosphotransferase System Modulates Virulence Gene Expression
Wang, Infection and immunity 2015 - “...ptsH C6706 ptsP (VC0672) C6706 fpr (VCA0518) C6706 VC1820 C6706 VCA0245 C6706 VC1822 C6706 VC2013 C6706 VC0995 C6706 VCA1045 C6706 VC0910 PtsI(H189A)...”
- “...mediated by particular EIIA family proteins (VC0964, VC1820, VCA0245, VC1822, and VCA1045) or EIIB/EIIC proteins (VC2013, VC0995, and VC0910) (42) (Fig....”
- Isolation and characterization of the new mosaic filamentous phage VFJ Φ of Vibrio cholerae
Wang, PloS one 2013 - “...+ + 1.510 5 VC2065 O1, El Tor, Inaba 1984 Shannxi + + + ND VC1820 O1, El Tor, Inaba 2001 Xinjiang + + + 3.310 7 VC3246 O1, El Tor, Ogawa 1977 Shandong + + 4.310 6 VC4421 O1, El Tor, Ogawa 1993 Liaoning +...”
- Mannitol and the mannitol-specific enzyme IIB subunit activate Vibrio cholerae biofilm formation
Ymele-Leki, Applied and environmental microbiology 2013 - “...IIABC mannitolspecific transporter (EIIMtl) (21, 35), and VC1820 and -1821 encoding hypothetical EIIA and EIIBC components, respectively, were the most highly...”
- Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germfree mouse intestine
Houot, Infection and immunity 2010 - “...of each of the following targeted genes (VC0672, VC1820, VC1822, VC1826, VCA1045, VCA0245, and VC1283) was amplified by PCR using the primer sets listed...”
- “...fragment fragment fragment of of of of of of of VC1820 VC1822 VC1826 VCA1045 VCA0245 VC0672 VC1283 the coding sequence of VC0965 the coding sequence of VC0966 a...”
- Three pathogenicity islands of Vibrio cholerae can excise from the chromosome and form circular intermediates
Murphy, Journal of bacteriology 2008 - “...Strain 1587 also contains an additional deletion of ORFs VC1820 to VC1828. In V. cholerae strain V51, a clinical isolate recovered in 1987 in the United States,...”
- “...Also, strain 1587 contains an additional deletion of ORFs VC1820 to VC1828, which suggests that isolates from South America may have a different origin. In V....”
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VFMJ11_A1060 bifunctional fructose-specific PTS IIA/HPr protein from Vibrio fischeri MJ11
VFMJ11_A1060 fused PTS fructose transporter subunit IIA/HPr protein from Aliivibrio fischeri MJ11
31% identity, 36% coverage
PTFAH_VIBCH / Q9KM70 Multiphosphoryl transfer protein; MTP from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see 2 papers)
VCA0518 PTS system, fructose-specific IIA/FPR component from Vibrio cholerae O1 biovar eltor str. N16961
30% identity, 33% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FruAB PTS system is involved in fructose transport.
disruption phenotype: Does not affect growth on glucose. Mutant shows a slight growth retardation on fructose. - Cra and cAMP Receptor Protein Have Opposing Roles in the Regulation of fruB in Vibrio cholerae
Beck, Journal of bacteriology 2021 - “...capable of both fructose and mannose transport ( 7 , 11 ). VCA0516, VCA0517, and VCA0518 make up the second locus encoding PTS Fru proteins ( 12 ). VCA0518 ( fruB ) encodes the fructose-specific EIIA domain and FPr, which exist as fusion proteins. VCA0517 (...”
- “...in minimal media. FIG 2 Map of the fruBKA locus and fruB promoter transcriptional fusions. VCA0518, VCA0517, and VCA0516 ( fruB , fruK , and fruA, respectively) encode components of the fructose-specific PTS in V. cholerae . VCA0519 ( cra ) encodes a putative transcriptional repressor....”
- Vibrio cholerae FruR facilitates binding of RNA polymerase to the fru promoter in the presence of fructose 1-phosphate
Yoon, Nucleic acids research 2021 - “...fructose PTS (PTS Fru ) consists of the membrane-spanning transporter FruA (VCA0516) and cytosolic FruB (VCA0518). It phosphorylates fructose to fructose 1-phosphate (F1P) during its translocation across the membrane. F1P is then converted to fructose 1,6-bisphosphate (FBP) by the 1-phosphofructokinase FruK (VCA0517). In V. cholerae ,...”
- Transcriptomics reveals a cross-modulatory effect between riboflavin and iron and outlines responses to riboflavin biosynthesis and uptake in Vibrio cholerae
Sepúlveda-Cisternas, Scientific reports 2018 - “...reductase 1.175 VCA0516 ptsIIB PTS system fructose-specific IIBC component 2.838 VCA0517 fruK 1-phosphofructokinase 1.948 1.919 VCA0518 ptsIIA PTS system fructose-specific IIA/FPR component 1.778 1.113 VCA0523 cqsA aminotransferase class II 2.585 VCA0540 formate transporter 1 putative 2.633 4.612 VCA0550 hypothetical protein 1.096 VCA0551 hypothetical protein 1.394 VCA0592...”
- “...ribD or ribN . However, three genes were differentially affected by the mutations. VCA0517 and VCA0518, encoded in an operon of a phosphotransferase system for fructose transport, were upregulated roughly 3.5 times in the ribD strain but downregulated 3.7 and 2.1 times respectively in the ribN...”
- A Genome-Wide Screen Reveals that the Vibrio cholerae Phosphoenolpyruvate Phosphotransferase System Modulates Virulence Gene Expression
Wang, Infection and immunity 2015 - “...C6706 cpdA ptsH C6706 ptsP (VC0672) C6706 fpr (VCA0518) C6706 VC1820 C6706 VCA0245 C6706 VC1822 C6706 VC2013 C6706 VC0995 C6706 VCA1045 C6706 VC0910 PtsI(H189A)...”
- “...pCVD442 carrying an unmarked, in-frame deletion in VCA0518 pCVD442 carrying an unmarked, in-frame deletion in VCA1820 pCVD442 carrying an unmarked, in-frame...”
- Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germfree mouse intestine
Houot, Infection and immunity 2010 - “...VC2533 pWM91 carrying an unmarked, in-frame deletion in VCA0518 pWM91 carrying an unmarked, in-frame deletion in VC2531 pWM91 carrying an unmarked, in-frame...”
- “...at 12 12 This study the coding sequence of VCA0518 a VCA0518 variant encoding an H-to-A mutation at This study This study a fragment of VCA0518 encoding...”
- Mining regulatory 5'UTRs from cDNA deep sequencing datasets
Livny, Nucleic acids research 2010 - “...Cold-shock domain-contain protein RLE0357(6) 29 VCA0819 Co-chaperonin GroES RLE0003(75) 30 VCA1075 Hypothetical protein RLE0037 31 VCA0518 Bifunctional fructose-specific PTS protein RLE0062(8) 32 VC2431 DNA topoisomerase IV subunit B RLE0226, SAM 33 VC2738 Phosphoenolpyruvate carboxykinase RLE0239(5) 34* VC1923 Trigger factor RLE0241(7) 35* VC1046 3-ketoacyl-CoA thiolase RLE0244(2) 36...”
- Proteins involved in difference of sorbitol fermentation rates of the toxigenic and nontoxigenic Vibrio cholerae El Tor strains revealed by comparative proteome analysis
Wang, BMC microbiology 2009 - “...more than five peptides matched and sequence coverage greater than 15%. Sequencing of the gene VCA0518 The gene VCA0518 (designated in the genome of N16961, GenBank Accession Number NC002506 ), which corresponds to the fructose-specific IIA/FPR component (PTS system, FIIA), was amplified from all tested strains...”
- “...B, mannitol-1-P dehydrogenase; C, pyruvate dehydrogenase; D, pyruvate formate-lyase 1 activating enzyme. Sequencing of the VCA0518 gene Due to the observed differences on the 2-DE gels (the VCA0518 gene product, FIIA component), the VCA0518 gene from all toxigenic and nontoxigenic strains studied were amplified and sequenced...”
WU75_14970 fused PTS fructose transporter subunit IIA/HPr protein from Vibrio parahaemolyticus
30% identity, 35% coverage
VPA0813 PTS system, fructose-specific IIA/FPR component from Vibrio parahaemolyticus RIMD 2210633
30% identity, 35% coverage
VpaChn25_0356 PTS mannitol transporter subunit IICBA from Vibrio parahaemolyticus
30% identity, 21% coverage
AT730_RS12975 PTS mannitol transporter subunit IICBA from Vibrio alginolyticus
30% identity, 21% coverage
- DUF1127-containing protein and ProQ had opposite effects on biofilm formation in Vibrio alginolyticus
Feng, BMC microbiology 2024 - “...UCUGUAGUAGAACGUACCGUUGUGAGUAUCAGCAUCUCGAAUA AT730_RS18585 retention module-containing protein sRNACandidate_2-12 0.50328 CGAAAUGUGACCAAGCAGAUCAUCCAUGUCGAUCAUUGAGUUAUUGAGAUCAGAGA AT730_RS18585 retention module-containing protein sRNACandidate_1-13 0.51842 CCACUUUUUACUUUACCAUCGAUGUA AT730_RS12975 mannitol PTS system EIICBA or EIICB component sRNACandidate_1-16 -3.6341 GCGAAUAUAUUCAUUUGGACAUCU AT730_RS10195 lysine decarboxylase sRNACandidate_2-15 -1.2173 CCCGGCCAGUCUCGAUUUCUACAAAGAAGUGG AT730_RS22505 glycosidase sRNACandidate_1-17 -0.7302 CAGUAUUUCUCUGGAUUCGUUUCGUGUCGCUGGC AT730_RS06365 multiple antibiotic resistance protein sRNACandidate_2-18 -1.0441 CCAAUAAAGGUCAUCGCCCAAUCACUGCUAUCAGACCAC AT730_RS22515...”
VP0370 PTS system, mannitol-specific IIABC component from Vibrio parahaemolyticus RIMD 2210633
30% identity, 21% coverage
LMOf6854_2852 PTS system, fructose-specific, IIABC component from Listeria monocytogenes str. 1/2a F6854
LMRG_01963 PTS system, fructose-specific, IIABC component from Listeria monocytogenes 10403S
33% identity, 22% coverage
- In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection
Camejo, PLoS pathogens 2009 - “...transcription regulator LMOf6854_2788 LMOf2365_2652 LMOh7858_2935 2,31 lmo2733 lmo2733 similar to PTS system, fructose-specific IIABC component LMOf6854_2852 LMOf2365_2720 LMOh7858_2997 2,35 lmo2736 lmo2736 unknown protein LMOf6854_2855 LMOf2365_2723 LMOh7858_3000 2,48 lmo1081 lmo1081 similar to glucose-1-phosphate thymidyl transferase LMOf6854_1134 8,25 5,99 5,17 lmo1082 lmo1082 similar to dTDP-sugar epimerase LMOf6854_1135 47,21...”
- Home Alone: Elimination of All but One Alternative Sigma Factor in Listeria monocytogenes Allows Prediction of New Roles for σB
Liu, Frontiers in microbiology 2017 - “...GATT GATT GTTT NF ATTT NF 10 signal TGGAAT TGGAAT TGGAAT TGGAGT NF TGGAAT NF LMRG_01963 35 signal GTTT GTTT GTTT NF NF NF NF 10 signal AGCAAT GGCAAT GGCAAT NF NF NF NF LMRG_01913 35 signal GTTT GTTT GTTT GTTT GTTT GTTT NF 10 signal...”
- Deep RNA sequencing of L. monocytogenes reveals overlapping and extensive stationary phase and sigma B-dependent transcriptomes, including multiple highly transcribed noncoding RNAs
Oliver, BMC genomics 2009 - “...proteins 2.63 4.87 1.79 LMRG_01151 lmo2003 similar to transcription regulator GntR family 14.67 3.15 0.32 LMRG_01963 lmo2733 similar to PTS system, fructose-specific IIABC component 7.95 1.35 0.32 Noncoding ND putative ncRNA, sbrE 186.09 2359.89 20.95 B -dependent genes with Average GEI 25 in 10403S Noncoding ND...”
llmg_0024 PTS system, mannitol-specific IIA component from Lactococcus lactis subsp. cremoris MG1363
33% identity, 88% coverage
ACIAD1990 phosphotransferase system, fructose-specific EI/HPr/EIIA components from Acinetobacter sp. ADP1
28% identity, 14% coverage
CD630_23320, CDIF630erm_02571 PTS sugar transporter subunit IIA from Clostridioides difficile 630
32% identity, 84% coverage
- Iron Regulation in Clostridioides difficile
Berges, Frontiers in microbiology 2018 - “...deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase 1.39 0.35 1.81 1.02 CD630_23310 CDIF630erm_02570 mtlD Mannitol-1-phosphate 5-dehydrogenase -6.28 -2.60 -5.79 -1.91 CD630_23320 CDIF630erm_02571 mtlF PTS system mannitol-specific transporter subunit IIA -5.98 OFF -5.53 -1.54 CD630_23330 CDIF630erm_02572 mtlR PTS operon transcription antiterminator -5.78 OFF -6.17 OFF CD630_23340 CDIF630erm_02573 mtlA PTS system mannitol-specific transporter...”
- “...reductase 1.39 0.35 1.81 1.02 CD630_23310 CDIF630erm_02570 mtlD Mannitol-1-phosphate 5-dehydrogenase -6.28 -2.60 -5.79 -1.91 CD630_23320 CDIF630erm_02571 mtlF PTS system mannitol-specific transporter subunit IIA -5.98 OFF -5.53 -1.54 CD630_23330 CDIF630erm_02572 mtlR PTS operon transcription antiterminator -5.78 OFF -6.17 OFF CD630_23340 CDIF630erm_02573 mtlA PTS system mannitol-specific transporter subunit...”
SP_0396 PTS sugar transporter subunit IIA from Streptococcus pneumoniae TIGR4
spr0358 Mannitol-specific enzyme IIA component from Streptococcus pneumoniae R6
SPD_0362 PTS system, mannitol-specific enzyme IIA from Streptococcus pneumoniae D39
31% identity, 86% coverage
CGSSpSV35_0555 PTS sugar transporter subunit IIA from Streptococcus pneumoniae SV35
31% identity, 86% coverage
- In vivo capsular switch in Streptococcus pneumoniae--analysis by whole genome sequencing
Hu, PloS one 2012 - “...(PTS) putative transcriptional regulator CGSSpSV35_0554 phosphotransferase system (PTS) putative mannitol-specific phosphotransferase system (PTS), IIA component CGSSpSV35_0555 phosphotransferase system (PTS) mannitol-1-phosphate 5-dehydrogenase CGSSpSV35_0556 phosphotransferase system (PTS) ABC-type polar amino acid transport system, ATPase component CGSSpSV35_0557 bacteriocin locus pncR CGSSpSV35_0662 bacteriocin locus pncT CGSSpSV35_0663 bacteriocin locus bacteriocin blpM...”
- “...transcriptional regulator CGSSpSV35_0554 phosphotransferase system (PTS) from SV35-T23 putative mannitol-specific phosphotransferase system (PTS), IIA component CGSSpSV35_0555 phosphotransferase system (PTS) from SV35-T23 mannitol-1-phosphate 5-dehydrogenase CGSSpSV35_0556 phosphotransferase system (PTS) from SV35-T23 ABC-type polar amino acid transport system, ATPase component CGSSpSV35_0557 bacteriocin locus from SV35-T23 pncR CGSSpSV35_0662 bacteriocin locus...”
GALLO_0995 Putative PTS system, mannitol-specific, IIA component from Streptococcus gallolyticus UCN34
SGGBAA2069_c09700, SGGB_0984 PTS sugar transporter subunit IIA from Streptococcus gallolyticus subsp. gallolyticus ATCC 43143
29% identity, 91% coverage
A0J47_RS08260 fused PTS fructose transporter subunit IIA/HPr protein from Photobacterium damselae subsp. damselae
28% identity, 36% coverage
AZOBR_RS32325 Fructose PTS system (E-I, HPr, and E-IIA components) from Azospirillum brasilense Sp245
31% identity, 14% coverage
- mutant phenotype: Specifically important for utilizing D-Fructose. This gene includes components E-I (PEP-protein phoshpotransferase), HPr, and E-IIA. Components E-IIB and E-IIC are in the adjacent gene AZOBR_RS32335.
TTE0181 Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) from Thermoanaerobacter tengcongensis MB4
27% identity, 81% coverage
Q45420 Mannitol-specific phosphotransferase enzyme IIA component from Geobacillus stearothermophilus
29% identity, 91% coverage
PTFAH_HAEIN / P44715 Multiphosphoryl transfer protein; MTP; Diphosphoryl transfer protein; DTP; Phosphotransferase FPr protein; Pseudo-HPr from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see paper)
HI0448 PTS system, fructose-specific IIA/FPr component (fruB) from Haemophilus influenzae Rd KW20
30% identity, 24% coverage
lmo2733 similar to PTS system, fructose-specific IIABC component from Listeria monocytogenes EGD-e
33% identity, 22% coverage
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...cellbiose transporter subunit IIA lmo2708 2.68 Yes/down 7.10 Yes/down lmo2708 PTS cellbiose transporter subunit IIC lmo2733 1.13 Yes/up 1.24 Yes/down lmo2733 PTS fructose transporter subunit IIABC lmo2762 2.93 Yes/down lmo2762 PTS cellbiose transporter subunit IIB lmo2763 2.32 Yes/down lmo2763 PTS cellbiose transporter subunit IIC lmo2765 2.78...”
- New Insights into the Lactic Acid Resistance Determinants of Listeria monocytogenes Based on Transposon Sequencing and Transcriptome Sequencing Analyses
Liu, Microbiology spectrum 2023 - “...cellobiose transporter subunit IIA 0.0828 6.78E-50 lmo2708 lmo2708 PTS cellobiose transporter subunit IIC 0.4097 3.16E-10 lmo2733 lmo2733 PTS fructose transporter subunit IIABC 0.4917 9.23E-05 lmo2762 lmo2762 PTS cellobiose transporter subunit IIB 0.0644 3.57E-06 lmo2763 lmo2763 PTS cellobiose transporter subunit IIC 0.1640 1.13E-16 lmo2765 lmo2765 PTS cellobiose...”
- YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes
Cheng, Gut microbes 2021 - “...Yes lmo2685 PTS cellbiose-specific enzyme IIB 2.16 Yes lmo2683 PTS cellbiose-specific enzyme IIB 2.09 Yes lmo2733 PTS fructose-specific enzyme IIABC 2.00 Yes lmo0914 PTS cellbiose-specific enzyme IIB 1.79 Yes lmo2373 PTS beta-glucoside-specific enzyme IIB 1.65 Yes lmo2772 PTS glucose/sucrose-specific enzyme IIB 1.52 Yes lmo1035 PTS beta-glucoside-specific...”
- Phosphotransferase system-dependent extracellular growth of listeria monocytogenes is regulated by alternative sigma factors σL and σH
Wang, Applied and environmental microbiology 2014 - “...pts genes, including lmo0021, lmo0027, lmo0398 to lmo0400, lmo2733, lmo0631, and lmo2665 (13, 14). The growth of L. monocytogenes is positively affected by the...”
- Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes
Hain, BMC genomics 2012 - “...systems ( lmo0021-lmo0024 , lmo0781-lmo0784 , lmo1997-lmo2002 ), two fructose specific systems ( lmo2335 and lmo2733 ), two galacitol specific systems ( lmo0503 , lmo0507 , lmo0508 and lmo2665-lmo2667 ), two beta-glucoside systems (the partial system lmo0373-lmo0374 and lmo0874-lmo0876 ), and two cellobiose specific systems (the...”
- Probing the pan-genome of Listeria monocytogenes: new insights into intraspecific niche expansion and genomic diversification
Deng, BMC genomics 2010 - “...associated with fructose-specific PTS enzyme II components ( lmo0357-0358 , lmo0631-0633 , lmo2135-2137 , and lmo2733 ). We surveyed the distribution of 978 annotated PTS genes and their homologs in all 26 L. monocytogenes genomes, and found 965 (99%) PTS genes are conserved in all LI...”
- “...fructose-specific enzyme IIA component IIIA + - lmo2143 Similar to mannose-6-phosphate isomerase IIIA - - lmo2733 Similar to PTS system, fructose-specific IIABC component IIIA + 494 lmo2734 Similar to sugar hydrolase IIIA + 494 lmo2735 Similar to Sucrose phosphorylase IIIA + 494 lmo2736 Hypothetical protein IIIA...”
- In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection
Camejo, PLoS pathogens 2009 - “...LMOf2365_2290 LMOh7858_2400 4,62 3,99 lmo2672 lmo2672 weakly similar to transcription regulator LMOf6854_2788 LMOf2365_2652 LMOh7858_2935 2,31 lmo2733 lmo2733 similar to PTS system, fructose-specific IIABC component LMOf6854_2852 LMOf2365_2720 LMOh7858_2997 2,35 lmo2736 lmo2736 unknown protein LMOf6854_2855 LMOf2365_2723 LMOh7858_3000 2,48 lmo1081 lmo1081 similar to glucose-1-phosphate thymidyl transferase LMOf6854_1134 8,25 5,99...”
- “...) -specific PTS encoding genes [63] were up regulated in vivo . Inversely, fructose ( lmo2733 ), galactitol ( lmo2665 ) and mannitol ( lmo2649 ) -specific PTS encoding genes appeared down regulated. Among the genes involved in bacterial ion uptake systems, a potassium-transporting ATPase encoding...”
- More
LMOf2365_2720 PTS system, fructose-specific, IIABC component from Listeria monocytogenes str. 4b F2365
LMOh7858_2997 PTS system, fructose-specific, IIABC component from Listeria monocytogenes str. 4b H7858
36% identity, 19% coverage
- In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection
Camejo, PLoS pathogens 2009 - “...regulator LMOf6854_2788 LMOf2365_2652 LMOh7858_2935 2,31 lmo2733 lmo2733 similar to PTS system, fructose-specific IIABC component LMOf6854_2852 LMOf2365_2720 LMOh7858_2997 2,35 lmo2736 lmo2736 unknown protein LMOf6854_2855 LMOf2365_2723 LMOh7858_3000 2,48 lmo1081 lmo1081 similar to glucose-1-phosphate thymidyl transferase LMOf6854_1134 8,25 5,99 5,17 lmo1082 lmo1082 similar to dTDP-sugar epimerase LMOf6854_1135 47,21 24,06...”
- In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection
Camejo, PLoS pathogens 2009 - “...LMOf6854_2788 LMOf2365_2652 LMOh7858_2935 2,31 lmo2733 lmo2733 similar to PTS system, fructose-specific IIABC component LMOf6854_2852 LMOf2365_2720 LMOh7858_2997 2,35 lmo2736 lmo2736 unknown protein LMOf6854_2855 LMOf2365_2723 LMOh7858_3000 2,48 lmo1081 lmo1081 similar to glucose-1-phosphate thymidyl transferase LMOf6854_1134 8,25 5,99 5,17 lmo1082 lmo1082 similar to dTDP-sugar epimerase LMOf6854_1135 47,21 24,06 21,01...”
C289_1394 PTS sugar transporter subunit IIA from Anoxybacillus ayderensis
27% identity, 91% coverage
- A high molecular-mass Anoxybacillus sp. SK3-4 amylopullulanase: characterization and its relationship in carbohydrate utilization
Kahar, International journal of molecular sciences 2013 - “...sugar transporters (C289_0465, C289_0466, C289_0467, C289_0603, C289_0763, C289_0764, C289_0765, C289_0778, C289_0779, C289_0780, C289_1015, C289_1174, C289_1392, C289_1394, C289_1910, C289_1911, and C289_1912) were also found in the Blast2GO annotation. The draft genome was submitted to the National Center for Biotechnology Information (NCBI) Bioproject with accession no. PRJNA174378. 2.2....”
TC 4.A.2.1.5 / C0H3V2 Mannitol-specific phosphotransferase enzyme IIA component, component of The mannitol porter (MtlA) (mannitol-1-P forming), MtlAF from Bacillus subtilis (strain 168)
BSU03982 phosphotransferase system (PTS) mannitol-specific enzyme IIA component from Bacillus subtilis subsp. subtilis str. 168
28% identity, 91% coverage
- substrates: mannitol
tcdb comment: The system is encoded by the mannitol catabolic operon, mtlAFD, and is regulated by the transcription factor, MtlR (Joyet et al. 2015). MtlR contains an N-terminal helix-turn-helix motif followed by an Mga-like domain, two PTS regulatory domains (PRDs), an Enzyme IIBGat-like domain and an Enzyme IIAMtl-like domain, the last four of which can be phosphorylated by the PTS. MtlR proteins are also found in Geobacillus stearothermophilus and Lactobacillus casei, but the mechanisms of their action are different (Joyet et al. 2015). The dephosphorlyated form of the protein activates transcription (Heravi and Altenbuchner 2014) - Linking Bacillus cereus Genotypes and Carbohydrate Utilization Capacity
Warda, PloS one 2016 - “...in any of the B . cereus strains. These include the mannitol cassette mtlAFD (BSU03981, BSU03982, BSU03900), the sorbitol/glucitol cassette gutRBP-fruC (BSU06140-BSU06170), the arabinan/arabinose cassette abnA-araABDLMNPQ-apfA (BSU28720-BSU28810), the L-rhamnose cassette rhaAMBR(EW) (BSU31180-BSU31220), and the oligomannoside cassette gmuBACDREFG (BSU05810-BSU05880). In agreement with this lack of genes, the...”
- T-REx: Transcriptome analysis webserver for RNA-seq Expression data
de, BMC genomics 2015 - “...green CodY R61K1 B_R61K T2 etc R61K2 B_R61K T2 BSU03981 red CcpA R61H1 C_R61K T2 BSU03982 red CcpA R61H2 C_R61K T2 BSU03990 red CcpA null1 Null T2 BSU04160 red CcpA null2 Null T2 BSU04470 red CcpA etc A) File describing the experiments and containing information of...”
VV1_0638 Mannitol/fructose-specific phosphotransferase system, IIA domain from Vibrio vulnificus CMCP6
28% identity, 22% coverage
- SOLiD sequencing of four Vibrio vulnificus genomes enables comparative genomic analysis and identification of candidate clade-specific virulence genes
Gulig, BMC genomics 2010 - “...Product Gene cog VV1_0251 hypothetical protein VV1_0251 - COG3094S VV1_0411 choline-glycine betaine transporter - COG1292M VV1_0638 mannitol/fructose-specific phosphotransferase system IIA protein - COG2213G VV1_0639 mannitol-1-phosphate 5-dehydrogenase - COG0246G VV1_0640 mannitol repressor protein mtlR COG3722K VV1_0641 D-fructose-6-phosphate amidotransferase - COG0449M VV1_0834 DMT family permease - - VV1_0835...”
- “...a complete mannitol catabolic pathway encoding the mannitol/fructose-specific phosphotransferase system IIA protein ( mtlABC , VV1_0638), mannitol-1-phosphate 5-dehydrogenase ( mtlD , VV1_0639), and a specific mannitol repressor ( mtlR , VV1_0640). The significance of these genes to virulence is unknown. Interestingly, by examining 465 V. vulnificus...”
VVMO6_02633 PTS mannitol transporter subunit IICBA from Vibrio vulnificus MO6-24/O
28% identity, 21% coverage
- Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains
Guerrero, PloS one 2019 - “...+ - VVA0333 + - VV1_1751 + + MO6-24/0 VVA0362 + + VV1_2031 - - VVMO6_02633 + + VVA0389 + - VV1_2037 - - VVMO6_02634 + + VVA0390 + - VV1_2038 - - VVMO6_02635 + + VVA0392 + - VV1_2061 + + VVMO6_03282 + - VVA0393...”
- Pyrosequencing-based comparative genome analysis of Vibrio vulnificus environmental isolates
Morrison, PloS one 2012 - “...activity+ * 2 VVMO6_03526 two-component system sensor protein GO:0004673 protein histidine kinase activity+ * 1 VVMO6_02633 PTS system, mannitol-specific IIC component GO:0016301 kinase activity+ * 1 VVMO6_02634 Mannitol-1-phosphate 5-dehydrogenase GO:0008926 mannitol-1-phosphate 5-dehydrogenase activity * 1 VVMO6_02635 Mannitol operon repressor N/A N/A YJ016 2 VVA0202 Transcriptional regulator...”
- “...each strain, with the exception of a small number of genes in V. vulnificus MO6-24/O (VVMO6_02633, VVMO6_02634, and VVMO6_02635). Based on the location of the E-genotype differentiating genes in the LCB alignments ( Figure 2 ), we were able to approximate the likely chromosomal location of...”
TC 4.A.2.1.12 / Q9KKQ7 The mannitol/glucitol transporter, MtlA (IICBAMtl) from Vibrio cholerae (see paper)
VCA1045 PTS system, mannitol-specific IIABC component from Vibrio cholerae O1 biovar eltor str. N16961
30% identity, 21% coverage
- substrates: Gluticol, mannitol
- Transcription of cis Antisense Small RNA MtlS in Vibrio cholerae Is Regulated by Transcription of Its Target Gene, mtlA
Zhang, Journal of bacteriology 2019 - “...V. cholerae life cycle. FIG 1 The mtl gene locus in V. cholerae . (A) VCA1045, VCA1046, and VCA1047 ( mtlA , mtlD , and mtlR , respectively) are three unique genes involved in the transport and/or metabolism of mannitol. MtlS is an antisense sRNA relative...”
- A Genome-Wide Screen Reveals that the Vibrio cholerae Phosphoenolpyruvate Phosphotransferase System Modulates Virulence Gene Expression
Wang, Infection and immunity 2015 - “...VCA0245 C6706 VC1822 C6706 VC2013 C6706 VC0995 C6706 VCA1045 C6706 VC0910 PtsI(H189A) VC0964(H91D) PtsH(H15A) Lab collection Lab collection 56 This study This...”
- “...VC0995 pCVD442 carrying an unmarked, in-frame deletion in VCA1045 pCVD442 carrying an unmarked, in-frame deletion in VC0910 pCVD442 carrying intact VC0965 with...”
- Roles of the sodium-translocating NADH:quinone oxidoreductase (Na+-NQR) on vibrio cholerae metabolism, motility and osmotic stress resistance
Minato, PloS one 2014 - “...up VCA0827 pterin-4-alpha-carbinolamine dehydratase 1.542 up 2.797 up VCA0948 hypothetical protein 2.456 up 1.554 up VCA1045 PTS system, mannitol-specific IIABC component 1.732 up 2.479 up VCA1046 mannitol-1-phosphate 5-dehydrogenase 1.997 up 1.712 up 10.1371/journal.pone.0097083.t002 Table 2 Genes down-regulated in the nqrA-F mutant based on microarray analysis. VC...”
- Mannitol and the mannitol-specific enzyme IIB subunit activate Vibrio cholerae biofilm formation
Ymele-Leki, Applied and environmental microbiology 2013 - “...Kmr, Apr pWM91 carrying an unmarked, in-frame deletion in VCA1045 (mtlA); Apr pWM91 carrying an unmarked, in-frame deletion in VCA1047 (mtlR); Apr 48 This study...”
- “...This study pFLAG-CTC carrying the coding sequence of VCA1045 pFLAG-CTC carrying a fragment of VCA1045 encoding positions 507 to 649 pFLAG-CTC carrying a variant...”
- The Vibrio cholerae mannitol transporter is regulated posttranscriptionally by the MtlS small regulatory RNA
Mustachio, Journal of bacteriology 2012 - “...mannitolspecific enzyme IIABC (EIIMtl) component (MtlA; VCA1045), a mannitol-1-phosphate dehydrogenase (MtlD; VCA1046), and a mannitol operon transcriptional...”
- Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germfree mouse intestine
Houot, Infection and immunity 2010 - “...of the following targeted genes (VC0672, VC1820, VC1822, VC1826, VCA1045, VCA0245, and VC1283) was amplified by PCR using the primer sets listed in Table 2,...”
- “...of of of of of of of VC1820 VC1822 VC1826 VCA1045 VCA0245 VC0672 VC1283 the coding sequence of VC0965 the coding sequence of VC0966 a VC0966 variant encoding an...”
- Experimental discovery of sRNAs in Vibrio cholerae by direct cloning, 5S/tRNA depletion and parallel sequencing
Liu, Nucleic acids research 2009 - “...2 B). This candidate sRNA is transcribed antisense to the 5-untranslated region (UTR) of mtlA (VCA1045), which encodes a transporter for mannitol, a naturally occurring six-carbon sugar alcohol. Using the sRNA-Seq data, we were able to map the 5 and 3-ends of IGR7; the transcript begins...”
- Identification of novel stage-specific genetic requirements through whole genome transcription profiling of Vibrio cholerae biofilm development
Moorthy, Molecular microbiology 2005 - “...VC1456 VC1457 VC2698 VC2699 VCA0817 VCA0818 VCA0820 VCA1045 VCA1046 Toxin co-regulated pilus biosynthesis protein P Toxin co-regulated pilus biosynthesis...”
LMRG_02196 PTS system IIA 2 domain protein from Listeria monocytogenes 10403S
lmo2651 similar to mannitol-specific PTS enzyme IIA component from Listeria monocytogenes EGD-e
LMON_2674 PTS sugar transporter subunit IIA from Listeria monocytogenes EGD
26% identity, 89% coverage
- An advanced bioinformatics approach for analyzing RNA-seq data reveals sigma H-dependent regulation of competence genes in Listeria monocytogenes
Liu, BMC genomics 2016 - “...() 1 Yes (81nt) coiA 8 lytG () 2 Yes (220nt) lytG 9 LMRG_02194 () LMRG_02196 , LMRG_02195 , LMRG_02193 , LMRG_02192 , LMRG_02191 , LMRG_02190 1 No No a Each fragment is composed of one or more 51nt overlapping windows b This indicates which genes...”
- Listeria monocytogenes GshF contributes to oxidative stress tolerance via regulation of the phosphoenolpyruvate-carbohydrate phosphotransferase system
Chen, Microbiology spectrum 2023 - “...5.48 Yes/down ulaA PTS system ascorbate transporter subunit IIC lmo2650 5.75 Yes/down lmo2650 MFS transporter lmo2651 2.43 Yes/down 5.06 Yes/down lmo2651 PTS mannitol transporter subunit IIA lmo2665 2.15 Yes/down lmo2665 PTS galacticol transporter subunit IIC lmo2666 - 3.38 Yes/down lmo2666 PTS galacticol transporter subunit IIB lmo2667...”
- DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress
Chen, Applied and environmental microbiology 2023 (secret) - New Insights into the Lactic Acid Resistance Determinants of Listeria monocytogenes Based on Transposon Sequencing and Transcriptome Sequencing Analyses
Liu, Microbiology spectrum 2023 - “...ulaA PTS system ascorbate transporter subunit IIC 0.0017 0.00E+00 lmo2650 lmo2650 MFS transporter 0.0101 6.86E-156 lmo2651 lmo2651 PTS mannitol transporter subunit IIA 0.0615 2.08E-143 lmo2665 lmo2665 PTS galacticol transporter subunit IIC 0.0033 0.00E+00 lmo2666 lmo2666 PTS galacticol transporter subunit IIB 0.0026 0.00E+00 lmo2667 lmo2667 PTS galacticol...”
- YjbH mediates the oxidative stress response and infection by regulating SpxA1 and the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) in Listeria monocytogenes
Cheng, Gut microbes 2021 - “...Yes lmo0425 PTS mannitol/fructose-specific enzyme IIA 4.11 Yes lmo2668 PTS mannitol/fructose-specific enzyme IIA 3.83 Yes lmo2651 PTS mannitol-specific enzyme IIA 3.78 Yes lmo0916 PTS lactose/cellobiose-specific enzyme IIA 3.06 Yes lmo0918 PTS mannitol/fructose-specific enzyme IIA 3.02 Yes lmo0402 PTS mannitol/fructose-specific enzyme IIA 2.95 Yes lmo0503 PTS fructose-specific...”
- Transcriptomic Analysis of the Adaptation of Listeria monocytogenes to Lagoon and Soil Matrices Associated with a Piggery Environment: Comparison of Expression Profiles
Vivant, Frontiers in microbiology 2017 - “...This included plant residues such as cellobiose ( lmo2708, lmo2762-63 and lmo2765 ), mannitol ( lmo2651 ), maltose and maltodextrin ( lmo2122-26 ), rhamnose, and rhamnulose ( lmo2847-49 ), residues of arthropods and fungi such as chitin ( lmo0105 and lmo1883 ) and residues of algae...”
- Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Hingston, PloS one 2017 - “...specific IIB subunit lmo2650 2 -2.19 -3.61 -1.53 -1.74 8.51 LMON_2674 PTS system, IIA component lmo2651 2 -2.41 -4.12 -1.54 -1.80 9.19 LMON_2706 PTS system, cellobiose-specific IIB component + lmo2683 17 -1.46 -1.30 1.32 -2.04 10.66 LMON_2707 PTS system, cellobiose-specific IIC component + lmo2684 17 -1.57...”
- Listeria monocytogenes 6-Phosphogluconolactonase mutants induce increased activation of a host cytosolic surveillance pathway
Crimmins, Infection and immunity 2009 - “...lmo0179 lmo0027 lmo1407 lmo1849 lmo1847 lmo1848 lmo0997 lmo2785 lmo2648 lmo2651 lmo2650 lmo2647 lmo0558 Fold changeb 71.6 9.8 8.1 7.2 6.9 4.4 3.8 3.3 3.2 3.0...”
- Glycerol metabolism and PrfA activity in Listeria monocytogenes
Joseph, Journal of bacteriology 2008 - “...lmo2114 lmo2115 arpJ lmo2390 lmo2469 glyA lmo2580 lmo2650b lmo2651 lmo2684 lmo2685 cydD cydC serS GC-B 5424 JOSEPH ET AL. J. BACTERIOL. incubated in...”
- Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Hingston, PloS one 2017 - “...8.78 LMON_2673 PTS system, lactose/cellobiose specific IIB subunit lmo2650 2 -2.19 -3.61 -1.53 -1.74 8.51 LMON_2674 PTS system, IIA component lmo2651 2 -2.41 -4.12 -1.54 -1.80 9.19 LMON_2706 PTS system, cellobiose-specific IIB component + lmo2683 17 -1.46 -1.30 1.32 -2.04 10.66 LMON_2707 PTS system, cellobiose-specific IIC...”
Tsac_2317 sigma 54-interacting transcriptional regulator from Thermoanaerobacterium saccharolyticum JW/SL-YS485
28% identity, 13% coverage
TTE0180 NtrC family Transcriptional regulator, ATPase domain from Thermoanaerobacter tengcongensis MB4
31% identity, 14% coverage
- Domain architectures of sigma54-dependent transcriptional activators
Studholme, Journal of bacteriology 2003 - “...by SwissProt/trEMBL accession numbers, except for XAC3643, TTE0180, and CPE23358, which are identified by their genome-specific open reading frame identifiers....”
- “...P. putida (Q52169 and O87788), T. tencongensis (TTE0180), and Xanthomonas campestris pv. Citri (XAC3643). VOL. 185, 2003 EBPs THAT ARE TWO-COMPONENT...”
VPA0231 phosphotransferase enzyme II, A component from Vibrio parahaemolyticus RIMD 2210633
WU75_00460 PTS sugar transporter subunit IIA from Vibrio parahaemolyticus
31% identity, 58% coverage
LicR / VIMSS821935 LicR regulator of Beta-glucosides utilization, effector HPr, phosphocarrier protein; LicB, lichenan-specific enzyme IIB PTS component (activator) from Bacillus clausii KSM-K16
28% identity, 20% coverage
BTF1_01615 BglG family transcription antiterminator from Bacillus thuringiensis HD-789
26% identity, 21% coverage
- NagRBt Is a Pleiotropic and Dual Transcriptional Regulator in Bacillus thuringiensis
Cao, Frontiers in microbiology 2018 - “...acacatctatacaact 12.12 17.301 21.967 0.785 BTF1_01610 Outer surface protein 156 agatgtatagacttgt 9.8 20.046 6.046 0.280 BTF1_01615 licR BigG family transcription antiterminator / / / 7.751 NT BTF1_06840 nupC nucleoside transporter NupC / / / 4.738 NT BTF1_06845 pdp Pyrimidine-nucleoside phosphorylase / / / 14.614 NT BTF1_06850...”
LPL9_RS14730 PTS sugar transporter subunit IIA from Lacticaseibacillus paracasei
27% identity, 92% coverage
Psest_3355 fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components from Pseudomonas stutzeri RCH2
30% identity, 11% coverage
- mutant phenotype: Specifically important for utilizing D-Fructose.
ECO26_3081 fused fructose-specific PTS enzymes: IIA component /HPr component from Escherichia coli O26:H11 str. 11368
29% identity, 35% coverage
llmg_0865 transcriptional antiterminator, BglG family from Lactococcus lactis subsp. cremoris MG1363
31% identity, 14% coverage
P23388 Multiphosphoryl transfer protein from Rhodobacter capsulatus
29% identity, 15% coverage
c0336 PTS system, mannitol (Cryptic)-specific IIA component from Escherichia coli CFT073
31% identity, 58% coverage
BC0806 Transcription antiterminator, BglG family from Bacillus cereus ATCC 14579
26% identity, 21% coverage
- Biochar immobilized plant growth-promoting rhizobacteria enhanced the physicochemical properties, agronomic characters and microbial communities during lettuce seedling
Guan, Frontiers in microbiology 2023 - “...phosphatase (ACP), and alkaline phosphatase (ALP) with the catalog number of BC0240, BC0120, BC0150, BC0100, BC0806, BC0880, BC0140, and BC0280, respectively. Agronomic and physicochemical properties of lettuce The plants were collected by gently shaking off the root soil and washing with distilled water. The surface moisture...”
BC_0806 BglG family transcription antiterminator from Bacillus cereus ATCC 14579
26% identity, 21% coverage
- Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579
Laouami, PloS one 2014 - “...0,471 1,480 0,004 NP_831256.1 ResD BC_1477 Transcriptional regulatory protein 0,356 0,008 0,599 0,119 NP_830596.1 - BC_0806 BigG family transcription antiterminator 0,485 0,276 1,922 0,000 Virulence-related proteins NP_834769.1 HlyI BC_5101 Hemolysin I 0,152 0,438 2,611 0,001 NP_832488.1 Npr2 BC_2735 Bacillolysin 1,089 0,193 2,120 0,013 NP_833332.1 HhoA BC_3600...”
TC 4.A.5.1.5 / D4GUP9 PTS system, galactitol-specific enzyme II, A component, putative, component of Galactitol enzyme II complex, IIAGat - IIBGat - IICGat (based on homology) from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (see paper)
HVO_2102 PTS system, galactitol-specific enzyme II, A component, putative from Haloferax volcanii DS2
34% identity, 77% coverage
LBCZ_0223 BglG family transcription antiterminator from Lacticaseibacillus casei DSM 20011 = JCM 1134 = ATCC 393
24% identity, 21% coverage
- Genomic adaptation of the Lactobacillus casei group
Toh, PloS one 2013 - “...LRHM_0149 LRHM_0156 6.1 carbohydrate transporter, two-componentsystem LBPC_0331 LBPC_0359 30.6 PTS transporter, amino acid ABC transporter LBCZ_0223 LBCZ_0252 33.8 carbohydrate utilization gene cluster LRHM_0172 LRHM_0177 6.6 taurine ABC transporter LBPC_0470 LBPC_0499 33.4 hypothetical protein LBCZ_0277 LBCZ_0286 8.9 conserved hypothetical protein LRHM_0256 LRHM_0268 14.8 myo-inositol catabolism protein LBPC_0579...”
STM3256 putative phosphotransferase system mannitol/fructose-specific IIA domain from Salmonella typhimurium LT2
35% identity, 37% coverage
- Comparative genomics of Salmonella enterica serovar Montevideo reveals lineage-specific gene differences that may influence ecological niche association
Nguyen, Microbial genomics 2018 - “...[ 46 ]. In addition, a tagatose utilization island tag , which spans STM3251 to STM3256 in Typhimurium [ 47 ], was found but restricted to members of clades I and II ( Fig. 2 ). Clades I and II are also distinguished by the presence...”
- Genetic Characterization of the Galactitol Utilization Pathway of Salmonella enterica Serovar Typhimurium
Nolle, Journal of bacteriology 2017 - “...function in tagatose uptake and metabolization (STM3254 to STM3256) that are absent from the second cluster. The second gat gene cluster, which is flanked...”
- “...of S. enterica serotype 4,[5],12:i: strain CVM23701 lacks STM3256, gatZ, gatA, gatB, and gatC. Thus, the reduced gat gene cluster in both strains sufficiently...”
- Short-term signatures of evolutionary change in the Salmonella enterica serovar typhimurium 14028 genome
Jarvik, Journal of bacteriology 2010 - “...a 5.1-kb deletion that removed at least four genes (STM3256 to STM3259) from the 14028s genome. Aside from genes encoded within prophages, there are no other...”
- Salmonella serovar identification using PCR-based detection of gene presence and absence
Arrach, Journal of clinical microbiology 2008 - “...STM3028, STM3036, STM3082, STM3120, STM3253, STM3254, STM3256, STM3257, STM3260, STM3355, STM3356, STM3357, STM3518, STM3529, STM3548, STM3549, STM3599,...”
- Genomic comparisons of Salmonella enterica serovar Dublin, Agona, and Typhimurium strains recently isolated from milk filters and bovine samples from Ireland, using a Salmonella microarray
Reen, Applied and environmental microbiology 2005 - “...SARB strain Du2. Similarly, region XXI (STM3251 to STM3256), a gene cluster involved in sugar metabolism, was 1619 GENOMIC COMPARISONS OF SALMONELLA STRAINS...”
Z3427 PTS system, fructose-specific IIA/fpr component from Escherichia coli O157:H7 EDL933
28% identity, 35% coverage
FruB / b2169 fructose-specific PTS multiphosphoryl transfer protein FruB from Escherichia coli K-12 substr. MG1655 (see 4 papers)
FruB / P69811 fructose-specific PTS multiphosphoryl transfer protein FruB from Escherichia coli (strain K12) (see 2 papers)
PTFAH_ECOLI / P69811 Multiphosphoryl transfer protein; MTP; Diphosphoryl transfer protein; DTP; Phosphotransferase FPr protein; Pseudo-HPr from Escherichia coli (strain K12) (see paper)
TC 4.A.2.1.1 / P69811 PTFA aka FRUB aka FRUF aka FPR aka B2169, component of Fructose porter (FruAB) (fructose-1-P forming) from Escherichia coli (see 5 papers)
b2169 fused fructose-specific PTS enzymes: IIA component/HPr component from Escherichia coli str. K-12 substr. MG1655
ECs3061 fructose-specific PTS system IIA component from Escherichia coli O157:H7 str. Sakai
28% identity, 35% coverage
- function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FruAB PTS system is involved in fructose transport.
- substrates: fructose
- CyuR is a dual regulator for L-cysteine dependent antimicrobial resistance in Escherichia coli
Rodionova, Communications biology 2024 - “...- 2.8 13 yebK b1853 DNA-binding transcriptional repressor 127 4.61 aAAaAAATaaCAACttTTTtaTC - - 14 fruB b2169 Fructose-specific PTS 192 4.58 GGAaAAATgGCAAaAAaTTgTgC 1.1 - 15 setB b2170 Sugar export transporter 198 4.58 GcAcAAtTTtTTGCcATTTtTCC 1.4 1.4 16 yfaL b2233 Putative autotransporter adhesin 277 4.79 GGcgAAATgGTTGtttTcTATTC - 2.4 17...”
- Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium
Cortés-Tolalpa, Microbial cell factories 2014 - “...Transport 19.9369 fecB b4290 Ferric dicitrate ABC transporter - periplasmic binding protein Transport 14.5864 fruB b2169 Fructose PTS permease - FruB subunit Transport 5.8528 glpT b2240 GlpT glycerol-3-P MFS transporter Transport 8.6190 lamB b4036 Phage lambda receptor protein; maltose high-affinity receptor Transport 18.0411 livK b3458 Leucine...”
- Antitoxin DinJ influences the general stress response through transcript stabilizer CspE
Hu, Environmental microbiology 2012 - “...2.6 DNA repair Endonuclease yeeS b2002 2.6 CP4-44 prophage; predicted DNA repair protein Transport fruB b2169 9.8 Fructosephosphotransferase enzyme III yddL b1472 4.0 Transport, OmpCFN porin family, N-terminal fragment Metabolism fruK b2168 4.0 Fructose-1-phosphate kinase; 1-phosphofructokinase ybdK b0581 4.3 Glutathione biosynthesis, cysteine ligase activity Cells at...”
- Directed evolution of ionizing radiation resistance in Escherichia coli
Harris, Journal of bacteriology 2009 - “...CB1000 contains a 3-bp in-frame deletion in the fruB (b2169; included in Table S4 in the supplemental material) coding sequence, and CB3000 carries a 93-bp...”
- Escherichia coli gene expression responsive to levels of the response regulator EvgA
Masuda, Journal of bacteriology 2002 - “...basR b4115 yjdE Decreased expression b0553 nmpC b2167 b2168 b2169 fruA fruK fruB b2943 galP Mean Outer membrane porin protein; locus of qsr prophage PTS system,...”
- Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations
Henderson, Genetics 1975 - “...is assumed that b2262 leaves REL intact while b1165, b2169 and b1319 delete at least enough of REL to render it inoperative in Int-promoted recombination. We...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...Theoreticalc Experimentald 4.85/38,938 (DIGE 4.5-6.5) P69811 Multiphosphoryl transfer protein 4.77/39,647.83 FtsA P0ABH0 Cell division protein 5.84/45,329.97...”
- Gene expression induced in Escherichia coli O157:H7 upon exposure to model apple juice
Bergholz, Applied and environmental microbiology 2009 - “...ECs1746 ECs2087 ECs2103 ECs2302 ECs2443 ECs2510 ECs2569 ECs3061 ECs3354 ECs3540 ECs3541 ECs3543 ECs3819 ECs4013 ECs4250 ECs4352 ECs4422 ECs4450 ECs4646 ECs4839...”
- Global transcriptional response of Escherichia coli O157:H7 to growth transitions in glucose minimal medium
Bergholz, BMC microbiology 2007 - “...ECs2463 ynjE putative thiosulfate sulfur transferase 3.47 1 ECs2614 yecH orf, hypothetical protein 3.09 6 ECs3061 fruB PTS system, fructose-specific IIA/fpr component 2.02 1 ECs3084 dsbE disulfide oxidoreductase 2.04 1 ECs3088 ccmC heme exporter protein C 2.19 1 ECs3090 ccmA ATP binding protein of heme exporter...”
- “...yeiT 2.14 3 ECs1037 rmf 2.24 2 ECs3060 fruK -2.13 1 ECs1072 O157 -2.13 5 ECs3061 fruB -2.38 1 ECs1091 O157 3.24 2 ECs3136 yfaX -2.04 1 ECs1137 yccC -2.27 1 ECs3149 menC -2.06 1 ECs1138 yccY -2.78 1 ECs3180 ackA -2.33 6 ECs1139 yccZ -2.74...”
t3176 phosphoryl transfer protein of PTS system from Salmonella enterica subsp. enterica serovar Typhi Ty2
35% identity, 37% coverage
c2704 PTS system, fructose-specific IIA/FPr component from Escherichia coli CFT073
28% identity, 35% coverage
CPn0061 PTS IIA Protein + HTH DNA-Binding Domain from Chlamydophila pneumoniae CWL029
28% identity, 45% coverage
- Growth of Chlamydia pneumoniae Is Enhanced in Cells with Impaired Mitochondrial Function
Käding, Frontiers in cellular and infection microbiology 2017 - “...1,977 Cpn0624 gapA glyceraldehyde-3-phosphate dehydrogenase 2,291 3,241 TRANSPORTER Cpn0023 yjjK ABC transporter ATPase 872 1,532 Cpn0061 ptsN_2 PTS IIA protein %2B HTH DNA-binding domain 410 733 Cpn0231 tauB nitrate%2Firon ABC transporter ATPase 934 1,470 Cpn0290 Na-dependent transporter 215 395 Cpn0486 proline permease 208 665 Cpn0536 dagA_1...”
SF5M90T_2187 fused PTS fructose transporter subunit IIA/HPr protein from Shigella flexneri 5a str. M90T
28% identity, 35% coverage
- RNA-seq analysis of the influence of anaerobiosis and FNR on Shigella flexneri
Vergara-Irigaray, BMC genomics 2014 - “...eda keto-hydroxyglutarate-aldolase/keto-deoxy-phosphogluconate aldolase 0.97 SF5M90T_580 galK galactokinase 0.95 0.62 SF5M90T_2913 tktA transketolase 1 isozyme 0.80 SF5M90T_2187 fruB PTS system fructose-specific transporter subunit IIA/HPr protein -1.33 SF5M90T_2186 fruK fructose-1-phosphate kinase -1.75 SF5M90T_3161 ptsO phosphocarrier protein NPr -1.76 SF5M90T_1637 putative transport protein -1.93 1.58 SF5M90T_2185 fruA PTS system,...”
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory