PaperBLAST
PaperBLAST Hits for SwissProt::E0SCY1 Glycine betaine/choline transport system ATP-binding protein OusV (Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))) (400 a.a., MAIKLEVTHL...)
Show query sequence
>SwissProt::E0SCY1 Glycine betaine/choline transport system ATP-binding protein OusV (Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)))
MAIKLEVTHLYKIFGEHPERAFRLLEQGLSKDQIFEKTGLTVGVKDASLAIEEGEIFVIM
GLSGSGKSTLVRLLNRLIEPTRGQVLIDGEDISRLPDGALRAVRRKQISMVFQSFALMPH
LNILDNTAFGMDLAGVPRAEREQKALNALQQVGLETYAHAYPDELSGGMRQRVGLARALA
NDPDILLMDEAFSALDPLIRTEMQDELIKLQARRQRTIVFISHDLDEAMRIGDRIAIMHS
GEVIQVGTPDEILNNPANDYVRTFFRGVDISHVFTAKDIARRRPVAVIRKTPGVGPRSAL
RILQEEDREYGYVLERGRKFIGVVSIDSLKQALRGQQPLEQALLPAPAPVPASMSLNELI
SQVAQAPCAVPVVDENHEYLGIISKGMLLQALDKESTLND
Running BLASTp...
Found 250 similar proteins in the literature:
OUSV_DICD3 / E0SCY1 Glycine betaine/choline transport system ATP-binding protein OusV from Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) (see paper)
100% identity, 100% coverage
- function: Part of the OusB ABC transporter complex involved in glycine betaine and choline uptake. May also transport several other osmoprotectants such as ectoine, DMSA, DMSP, proline and carnitine. Probably responsible for energy coupling to the transport system.
subunit: The complex is composed of two ATP-binding proteins (OusV), two transmembrane proteins (OusW) and a solute-binding protein (OusX).
disruption phenotype: Uptake of glycine betaine and choline is completely abolished in the ousA-ousB double mutant.
PROV_SALTY / P17328 Glycine betaine/proline betaine transport system ATP-binding protein ProV from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
STM2809 ABC superfamily (atp_bind), glycine/betaine/proline transport protein from Salmonella typhimurium LT2
SEN2654 glycine betaine/l-proline transport ATP-binding protein from Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
79% identity, 100% coverage
ProU / b2677 glycine betaine ABC transporter ATP binding subunit ProV (EC 7.6.2.9) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
proV / P14175 glycine betaine ABC transporter ATP binding subunit ProV (EC 7.6.2.9) from Escherichia coli (strain K12) (see 6 papers)
PROV_ECOLI / P14175 Glycine betaine/proline betaine transport system ATP-binding protein ProV from Escherichia coli (strain K12) (see 4 papers)
TC 3.A.1.12.1 / P14175 ProV aka B2677, component of Glycine betaine/proline porter, ProU or ProVWX (also transports proline betaine, carnitine, dimethyl proline, homobetaine, γ-butyrobetaine and choline with low affinity) from Escherichia coli (see 6 papers)
proV / GB|AAC75724.1 glycine betaine/l-proline transport atp-binding protein prov from Escherichia coli K12 (see 6 papers)
EcolC_1029 glycine betaine/L-proline ABC transporter, ATPase subunit from Escherichia coli C str. ATCC 8739
A0A140N655 Quaternary amine transport ATP-binding protein from Escherichia coli (strain B / BL21-DE3)
NP_417163 glycine betaine ABC transporter ATP binding subunit ProV from Escherichia coli str. K-12 substr. MG1655
b2677 glycine betaine transporter subunit from Escherichia coli str. K-12 substr. MG1655
B21_RS13195 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Escherichia coli BL21(DE3)
79% identity, 100% coverage
- function: Part of the ProU ABC transporter complex involved in glycine betaine and proline betaine uptake (PubMed:23249124, PubMed:3305496, PubMed:7898450). Probably responsible for energy coupling to the transport system (Probable).
subunit: The complex is composed of two ATP-binding proteins (ProV), two transmembrane proteins (ProW) and a solute-binding protein (ProX). - substrates: Carnitine, Choline, Dimethylproline, Gamma-butyrobetaine, Glycine betaine, Homobetaine, Proline
tcdb comment: Contributes to the regulation of cell volume is response to osmolarity. A reconsituted system shows osmotic strength-gating (Gul and Poolman 2012) - A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli
Xiao, BMC biotechnology 2017 - “...the proU operon, including proX (EcolC_1027, encoding substrate-binding protein), proW (EcolC_1028, encoding permease), and proV (EcolC_1029, encoding ATP-binding protein), increased 3- to 4- fold in RpoBD645Y. These results were consistent with previous observations that proline accumulation in E. coli was due to enhanced transportation and not...”
- A novel approach to evaluate ELISA antibody coverage of host cell proteins-combining ELISA-based immunocapture and mass spectrometry
Pilely, Biotechnology progress 2020 - “...Ab A Ab B Ab C pI MW (kDa) Guanylate kinase A0A140NBE7 5.7 85,357 Aminotransferase A0A140N655 5.4 44,163 SmpA/OmlA domain protein A0A140NFD8 6.6 15,689 DNAdirected RNA polymerase subunit beta A0A140NGD5 5.2 34,775 Thioredoxin A0A140NCS9 5.5 36,649 Betagalactosidase A0A140NDN8 6.5 37,999 DNA polymerase III, delta prime subunit...”
- RNase III initiates rapid degradation of proU mRNA upon hypo-osmotic stress in Escherichia coli.
Kavalchuk, RNA biology 2012 (PubMed)- GeneRIF: The proU mRNA is processed by RNase III and this processing is osmoregulated.
- Biodistribution of 89Zr-DFO-labeled avian pathogenic Escherichia coli outer membrane vesicles by PET imaging in chickens
Li, Poultry science 2023 - “...Cell wall/membrane/envelope biogenesis Cell inner membrane 430 P43341 LPXH Function unknown Cell inner membrane 431 P14175 PROV Amino acid transport and metabolism Cell inner membrane 432 P11350 NARI Energy production and conversion Cell inner membrane 433 P0AAT4 YBDG Cell wall/membrane/envelope biogenesis Cell inner membrane 434 P0AE82...”
- Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA
Sikkema, Science advances 2020 - “...UniProt IDs for the alignment are as follows: Ll-OpuAA, Q9KIF7; Bs-OpuAA, P46920; Lm-GbuA, Q9RR46; Ec-ProV, P14175; Ec-MetN, P30750; Cs-ArtN, Q8RCC2; and Ec-MalK, P68187. ( B ) HTH region of the NBD in the occluded conformation. The basic residues of KRIK are shown in stick representation in...”
- Superhelical destabilization in regulatory regions of stress response genes
Wang, PLoS computational biology 2008 - “...rpoD (P00579), gyrA (P09097), topA (P06612), hupA (P02342), himA (P06984), crp (P03020), lacI (P03023), proV (P14175), tyrP (P18199), ompC (P06996), fadL (P10384), ilvY (P05827), ilvC (P00510), cysB (P06613), narX (P10956), narL (P10957), narP (P31802), narQ (P27896), fnr (P03019), ArcA (P03026), and ArcB (P22763). Author contributions. HW...”
- Analysis of pleiotropic transcriptional profiles: a case study of DNA gyrase inhibition
Jeong, PLoS genetics 2006 - “...(P02931) , parC (P20082) , parE (P20083) , pdhR (P06957) , pps (P23538) , proV (P14175) , proW (P14176) , proX (P14177), rbsD (P04982) , recA (P03017) , rfaZ (P27241) , speB (P16936) , sulA (P08846) , tolC (P02930), topA (P06612) , topB (P14294) , trxA...”
- A genomic sample sequence of the entomopathogenic bacterium Photorhabdus luminescens W14: potential implications for virulence
Ffrench-Constant, Applied and environmental microbiology 2000 - “...S. typhimurium Borrelia burgdorferi P06202 P31132 P23847 Q47624 P30858 P14175 P45022 Q47538 U32782 AJ223978 P30296 AE000792 2 (0) 1 (0) 1 (0) 1 (0) 1 (0) 1...”
- Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions
Youngquist, Journal of industrial microbiology & biotechnology 2017 - “...subunit b3735 atpH 1.8 2.510 3 F1 sector of membrane-bound ATP synthase, delta subunit Osmoregulation b2677 proV 3.2 4.510 3 glycine betaine transporter subunit -ATP-binding component of ABC superfamily b2678 proW 3.3 8.710 5 glycine betaine transporter subunit - membrane component of ABC superfamily b2679 proX...”
- Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium
Cortés-Tolalpa, Microbial cell factories 2014 - “...b1244 Polypeptide: murein tripeptide ABC transporter/peptide ABC transporter - putative membrane subunit Transport 44.3550 proV b2677 Polypeptide: glycine betaine/proline ABC transporter - ATP binding subunit Transport 34.7488 proW b2678 Polypeptide: glycine betaine/proline ABC transporter - membrane subunitPolypeptide: glycine betaine/proline ABC transporter - membrane subunit Transport 35.6683...”
- Combinatorial strategies for improving multiple-stress resistance in industrially relevant Escherichia coli strains
Lennen, Applied and environmental microbiology 2014 - “...b2320 b0073 b2839 b0052 b0008 b0072 b0876 b0388 b4461 b4488 b2677 leuL leuD ilvA leuA pdxB leuB lysR pdxA talB leuC ybjD aroL yfjD ilvG proV leu operon leader...”
- Computational prediction of the osmoregulation network in Synechococcus sp. WH8102
Mao, BMC genomics 2010 - “...b0314 BetT Proline transporter b1896 OtsA Trehalose synthetases with UDPG as the b1897 OtsB substrate b2677 ProV Betaine/proline transporter b2678 ProW b2679 ProX b2938 SpeA Arginine synthetase b3404 EnvZ Two-component system regulating OmpC and b3405 OmpR OmpF under osmotic stress b0929 OmpF Porin-encoding genes b2215 OmpC...”
- “...E. coli b0314 BetT SYNW0229 SYNW0229-0233 0.99 E. coli b4111 ProP SYNW2494 SYNW2494-2495 E. coli b2677 ProV SYNW1915 SYNW1915-1917 0.56 E. coli b2678 ProW SYNW1916 SYNW1915-1917 0.99 E. coli b2679 ProX SYNW1917 SYNW1915-1917 0.99 E. coli AB094497 GsmT SYNW1914 PCC7418 AB094498 SdmT SYNW1913 PCC7418 sll1566 GgpS...”
- Genome-wide transcriptional responses of Escherichia coli K-12 to continuous osmotic and heat stresses
Gunasekera, Journal of bacteriology 2008 - “...participating in the osmotic-stressstimulated uptake of GB/proline (proU, b2677 to b2679) and K (kdpFABC, b4513 and b0696 to b0698) were in this cluster. All...”
- “...both temperatures. The genes of the proU operon (proVWX, b2677 to b2679) and the otsAB operon (b1896 and b1897), which encode a transport system for the...”
- A semi-supervised method for predicting transcription factor-gene interactions in Escherichia coli
Ernst, PLoS computational biology 2008 - “...1 Confirmed with direct binding evidence in Salmonella typhimurium [67] PhoB b4068, yjcH 1 NagC b2677, proV 1 FhlA b1924, fliD 1 LexA b1061, dinI 1 Yes Yes Gel shift assay and site-directed mutagenesis [68] ; ChIP-chip evidence [12] OxyR b4367, fhuF 1 DNaseI footprinting evidence...”
- DNA microarray analyses of the long-term adaptive response of Escherichia coli to acetate and propionate
Polen, Applied and environmental microbiology 2003 - “...1.38 0.43* 0.69 1.27 0.67 0.69 1.55* 1.02 1.78* b2677 b2678 b2679 proV proW proX 2 2 2 ATP-binding component of transport system High-affinity transport system...”
- Cross-Kingdom Comparative Transcriptomics Reveals Conserved Genetic Modules in Response to Cadmium Stress
Chen, mSystems 2021 - “...Log 2 FC p adjust Protein function Gene_id Log 2 FC p adjust Protein function B21_RS13195 <0.001 Glycine betaine/ l -proline ABC transporter YDR135C 0.81 <0.001 ABC type transporter YCF1 CHLRE_13g604150v5 0.60 <0.001 ABC transporter B21_RS17315 5.07 <0.001 Zn/Cd/Hg/Pb-transporting ATPase YDR039C 0.57 <0.001 K/Na ion transmembrane...”
- “...conservation of orthologous genes from different kingdoms associated with Cd stress, an ycf1 homologous gene B21_RS13195 of E. coli BL21, which showed a similarity of 34.44% to the ycf1 gene of yeast ( Table1 and Fig.5 ), was selected and overexpressed in competent cells of S....”
c3230 Glycine betaine/L-proline transport ATP-binding protein proV from Escherichia coli CFT073
79% identity, 100% coverage
Z_RS18625 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Escherichia coli O157:H7 str. EDL933
ECs3540 ATP-binding component of glycine betaine / proline transport system from Escherichia coli O157:H7 str. Sakai
78% identity, 100% coverage
- Transcriptomic Analysis of Viable but Non-Culturable Escherichia coli O157:H7 Formation Induced by Low Temperature
Zhong, Microorganisms 2019 - “...1 Z_RS26780, Z_RS26335, Z_RS25895, Z_RS25370, Z_RS24525, Z_RS24370, Z_RS23610, Z_RS23465, Z_RS22905, Z_RS21350, Z_RS21150, Z_RS21140, Z_RS21025, Z_RS19885, Z_RS18625, Z_RS16005, Z_RS12610, Z_RS12490, Z_RS09475, Z_RS09120, Z_RS04060, Z_RS03890, Z_RS02910, Z_RS02490, Z_RS01960, sec F, pst S, pst A, nep I, lam B, gld A, gcv T, gcv H, fuc I, fgh A,...”
- Identification and characterization of molecular entities differentially expressed in bacteria genome upon treatment with glyphosate shock
Fabunmi, Heliyon 2023 - “...genes were found to be differentially expressed ( Table 2 ). Glycine betaine transporter ATP-binding (ECs3540) and proline ABC transporter periplasmic binding (proV) was reduced considerably after glyphosate shock. A unique structural function of an osmoregulated ABC transporter (OpuA) that reacts to water stress has been...”
- “...[ 26 ]. This suggests that glyphosate slows the growth of some bacteria by blocking ECs3540 and causing hyperosmotic stress. Only two of the 30 genes were upregulated when cells were exposed to glyphosate shock, while the other 28 were downregulated ( Fig. 5 ). However,...”
- Physiological Response of Escherichia coli O157:H7 Sakai to Dynamic Changes in Temperature and Water Activity as Experienced during Carcass Chilling
King, Molecular & cellular proteomics : MCP 2016 - “...and binding proteins, including those involved in the uptake of glycine betaine/proline ( proV / ECs3540 , proW / ECs3541 ), transport proteins for charged solutes such as glutamate ( ECs0692 , ECs0693 ), a putrescine transporter ATP-binding subunit ( ECs0935 ) and histidine transport system...”
- Canonical Single Nucleotide Polymorphisms (SNPs) for High-Resolution Subtyping of Shiga-Toxin Producing Escherichia coli (STEC) O157:H7
Griffing, PloS one 2015 - “...(0.82), C (0.18) [ 21 ] ECs0418 445669 T (0.85), C (0.15) [ 21 ] ECs3540 3529080 C (0.85), T (0.15) [ 21 ] ECS0580_776 639975 G (0.86), T (0.14) [ 21 ] ECS2006_725 1984857 G (0.87), A (0.13) [ 21 ] ECs0721 * 797116 **...”
- Gene expression induced in Escherichia coli O157:H7 upon exposure to model apple juice
Bergholz, Applied and environmental microbiology 2009 - “...ECs2103 ECs2302 ECs2443 ECs2510 ECs2569 ECs3061 ECs3354 ECs3540 ECs3541 ECs3543 ECs3819 ECs4013 ECs4250 ECs4352 ECs4422 ECs4450 ECs4646 ECs4839 ECs5086 ECs5093...”
GM298_06810 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Enterobacter sp. HSTU-ASh6
78% identity, 99% coverage
KPN_03008 ATP-binding component of transport system for glycine, betaine and proline from Klebsiella pneumoniae subsp. pneumoniae MGH 78578
79% identity, 99% coverage
A0A077T7Z1 ABC-type quaternary amine transporter (subunit 1/3) (EC 7.6.2.9) from Vibrio anguillarum serotype O1 (see paper)
67% identity, 99% coverage
WU75_16165 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Vibrio parahaemolyticus
64% identity, 98% coverage
BAU10_06505 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Vibrio alginolyticus
64% identity, 98% coverage
- Identification of the global regulatory roles of RraA via the integrative transcriptome and proteome in Vibrio alginolyticus
Chen, mSphere 2024 - “...and S4). Additionally, several genes encoding ABC transporters were upregulated (BAU10_06495 ousX , up 2.05-fold; BAU10_06505 ousV , up 2.39-fold; BAU10_09575 oppF , up 2.16-fold; BAU10_20535 artP , up 4.18-fold); and several ABC transporters were downregulated (BAU10_05985 oppA , down 3.37-fold; BAU10_18030, down 6.38-fold; BAU10_19275 glnP...”
- “...down 3.37-fold), OusX (BAU10_06495, down 3.81-fold), OppA (BAU10_05985, down 24.48-fold), AotP (BAU10_03995, down 2.23-fold), OusV (BAU10_06505, down 3.29-fold), ArtP (BAU10_20535, down 1.78-fold), BAU10_18335(down 17.77-fold), SdaC (BAU10_20390, down 4.55-fold), OppA(BAU10_09280, down 1.86-fold), OppF (BAU10_05965, down 12.57-fold), and GlnQ (BAU10_07585, down 1.54-fold), suggesting that RraA may play a...”
VP1726 ABC superfamily (glycine/betaine/proline transport protein) from Vibrio parahaemolyticus RIMD 2210633
64% identity, 98% coverage
- The organosulfur compound dimethylsulfoniopropionate (DMSP) is utilized as an osmoprotectant by Vibrio species
Gregory, Applied and environmental microbiology 2021 (secret) - Investigations of Dimethylglycine, Glycine Betaine, and Ectoine Uptake by a Betaine-Carnitine-Choline Transporter Family Transporter with Diverse Substrate Specificity in Vibrio Species
Gregory, Journal of bacteriology 2020 (secret) - CosR Is a Global Regulator of the Osmotic Stress Response with Widespread Distribution among Bacteria
Gregory, Applied and environmental microbiology 2020 (secret) - Modulation of responses of Vibrio parahaemolyticus O3:K6 to pH and temperature stresses by growth at different salt concentrations
Whitaker, Applied and environmental microbiology 2010 - “...betaine/carnitine/choline transporter 1 (BCCT1) gene (VP1723), proU1 (VP1726), BCCT3 gene (VPA0356), and proU2 (VPA1109) transport systems after inorganic acid...”
- Osmoadaptation among Vibrio species and unique genomic features and physiological responses of Vibrio parahaemolyticus
Naughton, Applied and environmental microbiology 2009 - “...of intragenic in-frame mutations in ectB (VP1721) and proU1 (VP1726 to VP1728) in V. parahaemolyticus RIMD2210633, and the sizes of the regions removed from the...”
VpaChn25_1714 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Vibrio parahaemolyticus
64% identity, 98% coverage
VF_0787 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Aliivibrio fischeri ES114
VF_0787 glycine betaine transporter subunit from Vibrio fischeri ES114
63% identity, 98% coverage
SPISAL_06410 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Spiribacter salinus M19-40
58% identity, 98% coverage
- Compatible Solute Synthesis and Import by the Moderate Halophile Spiribacter salinus: Physiology and Genomics
León, Frontiers in microbiology 2018 - “...in all likelihood involved in compatible solute uptake; the OpuA and ProU systems (SPISAL_06400 to SPISAL_06410, and SPISAL_05285 to SPISAL_05290, respectively) ( Figure 2 ). The ProU transporter has been intensively studied both in E. coli and in S. typhimurium and is a binding-protein-dependent ABC-type uptake...”
- “...). The second predicted ABC-type osmostress protectant uptake system of S. salinus M19-40 (SPISAL_06400 to SPISAL_06410) is related to the OpuA glycine betaine transporter from B. subtilis ( Kempf and Bremer, 1995 ). The substrate-binding protein (OpuAC) from the Gram-positive bacterium B. subtilis is a lipoprotein...”
WP_011813050 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Halorhodospira halophila
57% identity, 98% coverage
pRL100079 putative glycine betaine/L-proline transport ATP-binding protein from Rhizobium leguminosarum bv. viciae 3841
56% identity, 96% coverage
BMEII0548 GLYCINE BETAINE/L-PROLINE TRANSPORT ATP-BINDING PROTEIN PROV from Brucella melitensis 16M
54% identity, 98% coverage
- Differential expression of iron acquisition genes by Brucella melitensis and Brucella canis during macrophage infection
Eskra, PloS one 2012 - “...BCAN_B0755 sugar-binding protein - NC 5.25 BMEII0544 BCAN_B0753 SN-glycerol-3-phosphate transport ATP-binding protein ugpC NC 4.46 BMEII0548 BCAN_B0748 glycine betaine/L-proline transport ATP-binding protein proV NC 3.32 BMEII0929 BCAN_B0318 ribonucleotide-diphosphate reductase subunit beta nrdF NC 3.41 BMEII0930 BCAN_B0317 ribonucleotide-diphosphate reductase subunit alpha - NC 6.43 BMEII0931 BCAN_B0316 ribonucleotide...”
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...Taurine import ABC BMEII0108, tauB BruAb21123 BRA1187 BOV_A1090 BCAN_B1217 86 OTCN Glycine betaine/L-proline import ABC BMEII0548 BruAb20492 BRA0740 BOV_A0692 BCAN_B0748 OTCN Glycine betaine/L-proline import IM BMEII0549 BruAb20493 BRA0739 BOV_A0691 BCAN_B0747 OTCN Glycine betaine/L-proline import BP BMEII0550 BruAb20494 BRA0738 BOV_A0690 BCAN_B0746 87 OTCN Nitrate import BP BMEII0797...”
BOV_A0692 glycine betaine/L-proline ABC transporter, ATP-binding protein from Brucella ovis ATCC 25840
BME_RS12870 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Brucella melitensis bv. 1 str. 16M
54% identity, 98% coverage
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...BMEII0108, tauB BruAb21123 BRA1187 BOV_A1090 BCAN_B1217 86 OTCN Glycine betaine/L-proline import ABC BMEII0548 BruAb20492 BRA0740 BOV_A0692 BCAN_B0748 OTCN Glycine betaine/L-proline import IM BMEII0549 BruAb20493 BRA0739 BOV_A0691 BCAN_B0747 OTCN Glycine betaine/L-proline import BP BMEII0550 BruAb20494 BRA0738 BOV_A0690 BCAN_B0746 87 OTCN Nitrate import BP BMEII0797 BruAb20753 BRA0469 BOV_A0406...”
- Survival characteristics and transcriptome profiling reveal the adaptive response of the Brucella melitensis 16M biofilm to osmotic stress
Guo, Frontiers in microbiology 2022 - “...B. melitensis 16M biofilm. Specifically, upregulated genes involved in the ABC transporter pathways included BME_RS06085, BME_RS12870, BME_RS13630, BME_RS12880, BME_RS1 2875, BME_RS01965, BME_RS02190, BME_RS06090, BME _RS02065, BME_RS02355, BME_RS02180, BME_RS11830. The expressions of these genes using qRT-PCR were in good agreement with the RNA-seq results ( Figures 5AC...”
BCAN_B0748 glycine betaine/L-proline transport ATP binding subunit from Brucella canis ATCC 23365
54% identity, 98% coverage
- Differential expression of iron acquisition genes by Brucella melitensis and Brucella canis during macrophage infection
Eskra, PloS one 2012 - “...sugar-binding protein - NC 5.25 BMEII0544 BCAN_B0753 SN-glycerol-3-phosphate transport ATP-binding protein ugpC NC 4.46 BMEII0548 BCAN_B0748 glycine betaine/L-proline transport ATP-binding protein proV NC 3.32 BMEII0929 BCAN_B0318 ribonucleotide-diphosphate reductase subunit beta nrdF NC 3.41 BMEII0930 BCAN_B0317 ribonucleotide-diphosphate reductase subunit alpha - NC 6.43 BMEII0931 BCAN_B0316 ribonucleotide reductase...”
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...tauB BruAb21123 BRA1187 BOV_A1090 BCAN_B1217 86 OTCN Glycine betaine/L-proline import ABC BMEII0548 BruAb20492 BRA0740 BOV_A0692 BCAN_B0748 OTCN Glycine betaine/L-proline import IM BMEII0549 BruAb20493 BRA0739 BOV_A0691 BCAN_B0747 OTCN Glycine betaine/L-proline import BP BMEII0550 BruAb20494 BRA0738 BOV_A0690 BCAN_B0746 87 OTCN Nitrate import BP BMEII0797 BruAb20753 BRA0469 BOV_A0406 BCAN_B0471...”
BRA0740 glycine betaine/L-proline ABC transporter, ATP-binding protein from Brucella suis 1330
54% identity, 98% coverage
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...ABC BMEII0108, tauB BruAb21123 BRA1187 BOV_A1090 BCAN_B1217 86 OTCN Glycine betaine/L-proline import ABC BMEII0548 BruAb20492 BRA0740 BOV_A0692 BCAN_B0748 OTCN Glycine betaine/L-proline import IM BMEII0549 BruAb20493 BRA0739 BOV_A0691 BCAN_B0747 OTCN Glycine betaine/L-proline import BP BMEII0550 BruAb20494 BRA0738 BOV_A0690 BCAN_B0746 87 OTCN Nitrate import BP BMEII0797 BruAb20753 BRA0469...”
BAB2_0500 ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase:Glycine betaine/L-proline transport ATP-binding subunit from Brucella melitensis biovar Abortus 2308
BAbS19_II04710 ATP/GTP-binding site motif A (P-loop) from Brucella abortus S19
54% identity, 96% coverage
TC 3.A.1.12.9 / Q8U4S5 OtaA, component of The salt-induced glycine betaine OtaABC transporter from Methanosarcina mazei (see 2 papers)
51% identity, 86% coverage
- substrates: Glycine betaine
TVD_RS10560 glycine betaine/L-proline ABC transporter ATP-binding protein ProV from Thioalkalivibrio versutus
54% identity, 97% coverage
BC2791 Glycine betaine transport ATP-binding protein from Bacillus cereus ATCC 14579
50% identity, 97% coverage
OPUAA_BACSU / P46920 Glycine betaine transport ATP-binding protein OpuAA; Quaternary-amine-transporting ATPase; EC 7.6.2.9 from Bacillus subtilis (strain 168) (see 2 papers)
P46920 ABC-type quaternary amine transporter (EC 7.6.2.9) from Bacillus subtilis (see paper)
TC 3.A.1.12.2 / P46920 OPAA aka OpuAA, component of Glycine betaine OpuAA/AB/AC porter (also transports dimethylsulfonioacetate and dimethylsulfoniopropionate) from Bacillus subtilis (see 4 papers)
52% identity, 93% coverage
- function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine. Probably responsible for energy coupling to the transport system.
catalytic activity: a quaternary ammonium(out) + ATP + H2O = a quaternary ammonium(in) + ADP + phosphate + H(+) (RHEA:11036)
subunit: The complex is composed of two ATP-binding proteins (OpuAA), two transmembrane proteins (OpuAB) and a solute-binding protein (OpuAC). - substrates: Dimethylsulfonioacetate, Dimethylsulfoniopropionate, Glycine betaine
tcdb comment: The system has been reconstituted in nanodiscs and shows substrate-dependent ionic stringth-gated gating and energy coupling dependent on anionic lipids (Karasawa et al. 2013) - In Silico Safety Assessment of <i>Bacillus</i> Isolated from Polish Bee Pollen and Bee Bread as Novel Probiotic Candidates
Bin, International journal of molecular sciences 2024 - “...OpuCA O34992 opuE Osmoregulated proline transporter OpuE O06493 opuAA Glycine betaine transport ATP-binding protein OpuAA P46920 opuAB Glycine betaine transport system permease protein OpuAB P46921 opuAC Glycine betaine-binding protein OpuAC P46922 Adhesion lspA Lipoprotein signal peptidase Q45479 spo0A Stage 0 sporulation protein A P06534 Tuf Elongation...”
- Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA.
Sikkema, Science advances 2020 - “...on the OpuA sequence. UniProt IDs for the alignment are as follows: Ll-OpuAA, Q9KIF7; Bs-OpuAA, P46920; Lm-GbuA, Q9RR46; Ec-ProV, P14175; Ec-MetN, P30750; Cs-ArtN, Q8RCC2; and Ec-MalK, P68187. ( B ) HTH region of the NBD in the occluded conformation. The basic residues of KRIK are shown...”
- The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
Ferreira, PloS one 2017 - “...MsmX), or orange (not conserved in MsmX). Accession numbers: MsmX (P94360), MalK (P68187), and OpuAA (P46920). (TIF) Click here for additional data file. S1 Appendix Construction of plasmids and B. subtilis strains. (DOCX) Click here for additional data file. Lia Godinho constructed pLG31, Graa Susete constructed...”
- A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study
Albaum, Proteome science 2011 - “...0.067524 <0.000001 1605 P02968 <0.000001 0.000001 <0.000001 <0.000001 9369 P37809 <0.000001 0.000002 0.007523 <0.000001 1112 P46920 <0.000001 >0.99 <0.000001 <0.000001 1149 P09124 <0.000001 >0.99 >0.99 <0.000001 685 P35136 <0.000001 >0.99 0.000003 <0.000001 1498 P37808 <0.000001 >0.99 >0.99 <0.000001 1219 P94356 <0.000001 0.037934 0.414572 <0.000001 2053 P80877...”
- “...<0.000001 <0.000001 2382 P02968 <0.000001 0.000001 <0.000001 <0.000001 9369 O31501 0.000036 0.006965 <0.000001 <0.000001 1681 P46920 <0.000001 >0.99 <0.000001 <0.000001 1149 O34966 <0.000001 0.012613 0.000188 <0.000001 1837 P35136 <0.000001 >0.99 0.000003 <0.000001 1498 P37808 <0.000001 >0.99 >0.99 <0.000001 1219 P94356 <0.000001 0.037934 0.414572 <0.000001 2053 P27206...”
- High-salinity-induced iron limitation in Bacillus subtilis
Hoffmann, Journal of bacteriology 2002 - “...MOWSE scorec Sequence coverage (%)c P39071 P45743 P40871 P02968 P46920 P21883 2.0e04 4.6e12 9.0e07 4.5e08 2.4e06 2.5e09 27 48 38 50 30 33 6.9e07 2.1e04 4.0e06...”
A21D_02672 glycine betaine/L-proline ABC transporter ATP-binding protein from Virgibacillus dokdonensis
50% identity, 100% coverage
Hore_21510 glycine betaine/L-proline ABC transporter, ATPase subunit from Halothermothrix orenii H 168
52% identity, 97% coverage
PAMH27_5169 glycine betaine/L-proline ABC transporter ATP-binding protein from Pseudomonas aeruginosa MH27
52% identity, 98% coverage
Q9KIF7 Glycine betaine transport ATP-binding protein OpuAA from Lactococcus lactis subsp. lactis (strain IL1403)
50% identity, 96% coverage
- The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact
van, eLife 2024 - “...Gene ( Lactococcus lactis IL1403) OpuAA NA Q9RQ05 Gene ( Lactococcus lactis IL1403) OpuABC NA Q9KIF7 Strain, strain background ( Lactococcus lactis ) Opu401 10.1073/pnas.0603871103 ; Biemans-Oldehinkel et al., 2006 Lactococcus lactis NZ9000 with OpuA gene deleted Strain, strain background ( Escherichia coli ) BL21(DE3) NA...”
- The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact
van, 2023 - Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA
Sikkema, Science advances 2020 - “...is based on the OpuA sequence. UniProt IDs for the alignment are as follows: Ll-OpuAA, Q9KIF7; Bs-OpuAA, P46920; Lm-GbuA, Q9RR46; Ec-ProV, P14175; Ec-MetN, P30750; Cs-ArtN, Q8RCC2; and Ec-MalK, P68187. ( B ) HTH region of the NBD in the occluded conformation. The basic residues of KRIK...”
DVU2299 glycine/betaine/L-proline ABC transporter, ATP binding protein from Desulfovibrio vulgaris Hildenborough
52% identity, 97% coverage
TC 3.A.1.12.5 / Q9RQ06 BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport from Lactococcus lactis (see paper)
llmg_1048 glycine betaine/proline ABC transporter from Lactococcus lactis subsp. cremoris MG1363
50% identity, 96% coverage
SPSF3K_00166 glycine betaine/L-proline ABC transporter ATP-binding protein from Streptococcus parauberis
50% identity, 94% coverage
SRX10_000873 glycine betaine/L-proline ABC transporter ATP-binding protein from Lacticaseibacillus paracasei
52% identity, 96% coverage
Bcep1808_3472 glycine betaine/L-proline ABC transporter, ATPase subunit from Burkholderia vietnamiensis G4
49% identity, 98% coverage
- Differential Expression and PAH Degradation: What Burkholderia vietnamiensis G4 Can Tell Us?
Cauduro, International journal of microbiology 2020 - “...Bcep1808_3624, Bcep1808_5570, and Bcep1808_5571), oligopeptide/dipeptide ABC transport ATPase (Bcep1808_3703, Bcep1808_3703), glycine betaine/L-proline ABC transport ATPase (Bcep1808_3472), and ABC transporter (Bcep1808_5572) were all found to have a similar function. These proteins carry solutes and toxins through the membrane, regardless of the concentration gradient. Such movement occurs via...”
SSA_0386 Glycine-betaine ABC transporter, ATPase component, putative from Streptococcus sanguinis SK36
49% identity, 97% coverage
M5005_Spy_0157 glycine betaine transport ATP-binding protein from Streptococcus pyogenes MGAS5005
SPy0183 putative glycine betaine/proline ABC transporter (ATP-binding protein) from Streptococcus pyogenes M1 GAS
SpyM3_0143 putative glycine betaine/proline ABC transporter from Streptococcus pyogenes MGAS315
50% identity, 98% coverage
- TrxR, a new CovR-repressed response regulator that activates the Mga virulence regulon in group A Streptococcus
Leday, Infection and immunity 2008 - “...M5005_Spy_0624 M5005_Spy_0985 M5005_Spy_1758 M5005_Spy_0694 M5005_Spy_0157 M5005_Spy_0158 Ribosome-binding factor A, putative N utilization substance...”
- New Pathogenesis Mechanisms and Translational Leads Identified by Multidimensional Analysis of Necrotizing Myositis in Primates
Kachroo, mBio 2020 - “...Spy1826 12.0 29.2 Spy1853 hasC UTPglucose-1-phosphate uridylyltransferase Spy1827 5.9 10.5 Cytolysin/exotoxin Spy0139 nga NAD glycohydrolase Spy0183 5.2 35.1 Spy0140 ifs Immunity factor for SPN Spy0184 6.1 30.1 Spy0141 slo Streptolysin O Spy0185 5.7 27.8 Spy0351 spyA c3 family ADP-ribosyltransferase Spy0403 7.4 11.5 Spy0562 sagA Streptolysin S...”
- Novel conserved group A streptococcal proteins identified by the antigenome technology as vaccine candidates for a non-M protein-based vaccine
Fritzer, Infection and immunity 2010 - “...F:2, I:16, K:24, N:29, P:12 Spy0168 Spy0171 Spy0183 Spy0230 Spy0269 Hypothetical protein Hypothetical protein Putative glycine betaine/proline ABC transporter...”
- Defects in ex vivo and in vivo growth and sensitivity to osmotic stress of group A Streptococcus caused by interruption of response regulator gene vicR
Liu, Microbiology (Reading, England) 2006 - “...and up-regulated expression of 5 genes, including spy0183 and spy0184, which encode an osmoprotectant transporter OpuA. Consistent with microarray analysis, the...”
- “...control preference of nutrient utilization. NIH-PA Author Manuscript SPy0183 and SPy0184 share 67% and 58% identity in amino acid sequence with OpuAA and...”
- PolA1, a putative DNA polymerase I, is coexpressed with PerR and contributes to peroxide stress defenses of group A Streptococcus
Toukoki, Journal of bacteriology 2013 - “...was used to obtain cDNA for determining the spyM3_0143 initiation site, while primers 0146GSP1 and 0147GSP5 were used independently to generate cDNA for...”
- “...Fig. 1B, amplification of intergenic and coding sequences between spyM3_0143 and perR from cDNA (products 2, 3, 4, and 5) suggested the existence of a...”
LRHM_RS10305 glycine betaine/L-proline ABC transporter ATP-binding protein from Lacticaseibacillus rhamnosus GG
51% identity, 96% coverage
EF2641 glycine betaine/L-proline ABC transporter, ATP-binding subunit from Enterococcus faecalis V583
50% identity, 99% coverage
- Transcriptomic and functional analysis of NaCl-induced stress in Enterococcus faecalis
Solheim, PloS one 2014 - “...binding proteins EF0865 0,17 1,38 1,07 Glycine betaine/L-proline ABC transporter,ATP-binding subunit Transport and binding proteins EF2641 0,21 3,42 2,92 Glycine betaine/L-proline ABC transporter, glycine betaine/L-proline-binding/permease protein Transport and binding proteins EF2642 0,69 4,99 4,21 Glyoxylase family protein Unknown function EF2214 1,22 1,20 1,83 Log 2 ratios...”
- “...transporters predicted to be involved in the uptake of glycine betaine (EF0862 to -65 and EF2641 to -42) may suggest that this is the predominant compatible solute taken up by V583 when grown in BHI. Sodium extrusion by bacteria is generally attributed to antiport of Na...”
- Bicarbonate enhances expression of the endocarditis and biofilm associated pilus locus, ebpR-ebpABC, in Enterococcus faecalis
Bourgogne, BMC microbiology 2010 - “...of a mannitol PTS system. EF2642 also appeared to be expressed in an operon with EF2641, which was also activated (4.1-fold, P < 0.05). EF2641 and EF2642 encode a putative glycine betaine/L-proline ABC transporter ATP-binding protein and permease protein, respectively. Those results were confirmed by qRT-PCR...”
- “...with a decrease of 32-fold for EF0082 in the presence of bicarbonate while EF0411 and EF2641 expression levels increased in the presence of bicarbonate by 24-fold and 8.5-fold, respectively (results not shown). The ebpR-ebpABC locus did not appear to be affected in these conditions (late log...”
- Transcriptional response of Enterococcus faecalis V583 to erythromycin
Aakra, Antimicrobial agents and chemotherapy 2005 - “...protein protein protein protein protein EF2047 EF2058 EF2059 EF2226 EF2364 EF2377 EF2485 EF2509 EF2641 EF2642 0 0 0 0 0.73 0 0 0 0 0 0 0 0 0 0 0.63 0 0 0 0 0.90...”
7aheC / Q9KIF7 Opua inhibited inward facing (see paper)
49% identity, 98% coverage
- Ligand: (2r,3r,3as,5r,7ar,9r,10r,10as,12r,14ar)-2,9-bis(6-amino-9h-purin-9-yl)octahydro-2h,7h-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide (7aheC)
BTH_II0966 glycine betaine/L-proline ABC transporter, ATP-binding protein from Burkholderia thailandensis E264
48% identity, 68% coverage
LSA1696 Putative glycine/betaine/carnitine ABC transporter, ATP-binding subunit from Lactobacillus sakei subsp. sakei 23K
49% identity, 98% coverage
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...lipoprotein precursor -1.7 -1.1 LSA1695 lsa1695 Putative glycine/betaine/carnitine ABC transporter, membrane-spanning subunit -2.1 -2.0 -1.9 LSA1696 lsa1696 Putative glycine/betaine/carnitine ABC transporter, ATP-binding subunit -1.6 -0.9 LSA1870 lsa1870 Putative glycine betaine/carnitine/choline ABC transporter, ATP-binding subunit -0.6 -0.6 Protein modification LSA0865 lsa0865 Putative protein methionine sulfoxide reductase -0.6...”
Dde_0673 ATP-binding component of transport system for glycine, betaine and proline from Desulfovibrio desulfuricans G20
50% identity, 97% coverage
- Transcriptomics and Functional Analysis of Copper Stress Response in the Sulfate-Reducing Bacterium Desulfovibrio alaskensis G20
Tripathi, International journal of molecular sciences 2022 - “...to the class translocase and is involved in the translocation of inorganic cations. In SP2, Dde_0673 (glycine betaine/L-proline ABC transporter, ATPase subunit) was the most upregulated gene with three GO-MF terms assigned. In both SP1 and SP2, the most upregulated genes ( Figure 5 c,d) were...”
- “...(branched-chain amino acid transport system permease protein (LivH)). In addition, other ABC-type amino acid transporters (Dde_0673, Dde_3743, Dde_1430) were also upregulated in SP2. These genes code for glycine/betaine/L-proline ABC transporter (Dde_0673) and polar amino acid ABC transporter (Dde_3743 and Dde_1430). The same amino acid ABC transporters...”
PSPTO_3060 glycine betaine/L-proline ABC transporter, ATP-binding protein from Pseudomonas syringae pv. tomato str. DC3000
62% identity, 69% coverage
WP_009292952 glycine betaine/L-proline ABC transporter ATP-binding protein from Bacteroides fragilis
48% identity, 93% coverage
GBUA_LISM4 / Q9RR46 Glycine betaine/carnitine transport ATP-binding protein GbuA; EC 7.6.2.9 from Listeria monocytogenes serotype 1/2a (strain 10403S) (see 4 papers)
lmo1014 highly similar to glycine betaine ABC transporter (ATP-binding protein) from Listeria monocytogenes EGD-e
LMON_1024 glycine betaine/L-proline ABC transporter ATP-binding protein from Listeria monocytogenes EGD
48% identity, 97% coverage
- function: Part of the ABC transporter complex GbuABC involved in glycine betaine uptake. Responsible for energy coupling to the transport system. Involved, with BetL and OpuC, in osmoprotection and cryoprotection of Listeria. Can also uptake carnitine when carnitine is abundant in the growth medium.
catalytic activity: a quaternary ammonium(out) + ATP + H2O = a quaternary ammonium(in) + ADP + phosphate + H(+) (RHEA:11036)
subunit: The complex is composed of two ATP-binding proteins (GbuA), two transmembrane proteins (GbuB) and a solute-binding protein (GbuC).
disruption phenotype: Mutants are impaired in the transport of glycine betaine at elevated osmolarity and at decreased temperature. - Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA.
Sikkema, Science advances 2020 - “...OpuA sequence. UniProt IDs for the alignment are as follows: Ll-OpuAA, Q9KIF7; Bs-OpuAA, P46920; Lm-GbuA, Q9RR46; Ec-ProV, P14175; Ec-MetN, P30750; Cs-ArtN, Q8RCC2; and Ec-MalK, P68187. ( B ) HTH region of the NBD in the occluded conformation. The basic residues of KRIK are shown in stick...”
- The population structure and genetic diversity of Listeria monocytogenes ST9 strains based on genomic analysis
Song, Frontiers in microbiology 2022 - “...lmo2391, lmo0039, lmo0037, lmo0913, lmo0036 , and lmo1367 ), high salt concentration ( lmo2748, lmo2092, lmo1014, lmo1015, lmo1016, lmo1428, lmo1427, lmo1426 , and lmo1425 ), and biofilm formation ( lmo0673, lmo0435, lmo1460, lmo2504, lmo1288 , and inlL ), and SSI-1 containing five genes ( lmo0444lmo0448 )...”
- “...cspB, cspD, lmo0866, lmo1722, lmo1378 , and lmo0288 ), high salt concentration ( lmo2748, lmo2092, lmo1014, lmo1015, lmo1016, lmo1428, lmo1427, lmo1426 , and lmo1425 ), and SSI-1 ( lmo0444, lmo0445, lmo0446, lmo0447 , and lmo0448 ) are shown. Click here for additional data file. Supplementary Figure...”
- Different Shades of Listeria monocytogenes: Strain, Serotype, and Lineage-Based Variability in Virulence and Stress Tolerance Profiles
Muchaamba, Frontiers in microbiology 2021 - “...of genetic background- based SNPs or InDels c BetL lmo2092 Glycine betaine transporter 10 Gbu lmo1014 lmo1016 Glycine betaine transporter 5 OpuC lmo1425 lmo1428 Carnitine transporter 4 KdpED lmo2678 lmo2679 Kdp ATPase, two-component system for K+ uptake 36 KdpABC lmo2680 lmo2682 Potassium-transporting ATPase 21 ProBA lmo1259...”
- c-di-AMP modulates Listeria monocytogenes central metabolism to regulate growth, antibiotic resistance and osmoregulation
Whiteley, Molecular microbiology 2017 - “...; oppB , lmo2195 , LMRG_01637 ; relA , lmo1523 , LMRG_01447 ; gbuA , lmo1014 , LMRG_02114 ; pstA , lmo2692 , LMRG_02239 ; cbpB , lmo1009 , LMRG_02109 ; pycA , lmo1072 , LMRG_00534 ; citZ , lmo1567 , LMRG_01400 ; citC , lmo1566...”
- Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Hingston, PloS one 2017 - “...G5 Osmolyte and oligopeptide uptake LMON_1024 Glycine betaine ABC transport system, ATP-binding protein gbuA + lmo1014 8 0.15 0.56 0.82 1.68 -0.69 LMON_1025 Glycine betaine ABC transport system, permease protein gbuB + lmo1015 19 0.04 0.48 0.75 1.14 0.08 LMON_1026 Glycine betaine ABC transport system, glycine...”
- Cyclic di-AMP targets the cystathionine beta-synthase domain of the osmolyte transporter OpuC
Huynh, Molecular microbiology 2016 - “...CBS domain of the carnitine transporter component OpuCA. (A) Domain organization of OpuCA (Lmo1428), GbuA (Lmo1014), and BilE (Lmo1421). (B) Binding of OpuCA, GbuA, and BilE to c-di-AMP as measured by DRaCALA to full-length (FL), ATP-binding (ATP), or CBS domains. (C) Binding titration for the OpuCA...”
- Listeria monocytogenes shows temperature-dependent and -independent responses to salt stress, including responses that induce cross-protection against other stresses
Bergholz, Applied and environmental microbiology 2012 - “...LMOh7858_3052 LMOh7858_3076 lmo0201 lmo0211 lmo0433 lmo0955 lmo1014 lmo1022 lmo1428 lmo1473 lmo1580 lmo2067 lmo2069 lmo2092 lmo2196 lmo2748 lmo2754 lmo2785...”
- Transcriptome analysis of alkali shock and alkali adaptation in Listeria monocytogenes 10403S
Giotis, Foodborne pathogens and disease 2010 - “...Similar to betaine=carnitine=choline ABC transporter (membrane p) lmo1014 gbuA 10 2.4 2.77 Highly similar to glycine betaine ABC transporter (ATP-binding...”
- Deciphering the intracellular metabolism of Listeria monocytogenes by mutant screening and modelling
Schauer, BMC genomics 2010 - “...system enzyme I no 3.4 1.0 8 purS (lmo1771) phosphoribosylformylglycinamidine synthetase rf 11.0 1.0 gbuA (lmo1014) glycine betaine ABC transporter, ATP-binding protein rf 10.0 4.0 pyrE (lmo1831) orotatephosphoribosyltransferase no 5.5 2.5 8 lmo1416 hypothetical transporter no 5.3 1.0 metabolism of lipids 6 opuCA (lmo1428) glycine betaine/carnitine/choline...”
- More
- Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Hingston, PloS one 2017 - “...2 DE across five growth phases G1 G2 G3 G4 G5 Osmolyte and oligopeptide uptake LMON_1024 Glycine betaine ABC transport system, ATP-binding protein gbuA + lmo1014 8 0.15 0.56 0.82 1.68 -0.69 LMON_1025 Glycine betaine ABC transport system, permease protein gbuB + lmo1015 19 0.04 0.48...”
BP2057 putative ABC-transporter ATP-binding protein from Bordetella pertussis Tohama I
48% identity, 91% coverage
ArtHe_04675 glycine betaine/L-proline ABC transporter ATP-binding protein from Arthrobacter sp. Helios
47% identity, 90% coverage
- Transcriptional response of the xerotolerant Arthrobacter sp. Helios strain to PEG-induced drought stress
Hernández-Fernández, Frontiers in microbiology 2022 - “...transport genes seems to be present in the Helios strain genome sequence. The ArtHe_04665 - ArtHe_04675 cluster codes two ABC transporter permeases and one ATP binding protein, respectively. Glycine betaine can be synthesized from choline by a two-step pathway with betaine aldehyde as intermediate. The coding...”
- “...shown in Table 1 , Arthrobacter sp. Helios facing water shortage induced the expression of ArtHe_04675 and ArtHe_04665 , genes coding glycine betaine ABC transporters involved in the uptake of this compatible solute. The highest induction (>50 fold) was observed in the kdpA ( ArtHe_17390 )...”
Ddes_0824 glycine betaine/L-proline ABC transporter, ATPase subunit from Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774
46% identity, 97% coverage
HQ775_RS10910, HQ843_RS19795 glycine betaine/L-proline ABC transporter ATP-binding protein from Martelella sp. NC18
46% identity, 96% coverage
- Genomic Analysis of Halotolerant Bacterial Strains Martelella soudanensis NC18T and NC20
Lee, Journal of microbiology and biotechnology 2022 - “...HQ843_RS28025 pepQ K01271 Xaa-Pro dipeptidase HQ775_RS07200 HQ843_RS23495 proV K02000 glycine betaine transport system ATP-binding protein HQ775_RS10910 HQ843_RS09775, HQ843_RS05790, HQ843_RS19795 proW K02001 glycine betaine transport system permease protein HQ775_RS24955, HQ775_RS10905 HQ843_RS05795, HQ843_RS19800 proX K02002 glycine betaine transport system substrate-binding protein HQ775_RS10900 HQ843_RS19805 TC.BCT K03451 betaine/carnitine transporter, BCCT...”
- “...Xaa-Pro dipeptidase HQ775_RS07200 HQ843_RS23495 proV K02000 glycine betaine transport system ATP-binding protein HQ775_RS10910 HQ843_RS09775, HQ843_RS05790, HQ843_RS19795 proW K02001 glycine betaine transport system permease protein HQ775_RS24955, HQ775_RS10905 HQ843_RS05795, HQ843_RS19800 proX K02002 glycine betaine transport system substrate-binding protein HQ775_RS10900 HQ843_RS19805 TC.BCT K03451 betaine/carnitine transporter, BCCT family HQ775_RS23930 HQ843_RS06820...”
D7DRC5 Glycine betaine/L-proline ABC transporter, ATPase subunit from Methanococcus voltae (strain ATCC BAA-1334 / A3)
43% identity, 95% coverage
PA5094 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa PAO1
61% identity, 65% coverage
PA14_67270 putative ATP-binding component of ABC transporter from Pseudomonas aeruginosa UCBPP-PA14
61% identity, 65% coverage
AO356_09610 ABC transporter for L-Histidine, ATPase component from Pseudomonas fluorescens FW300-N2C3
61% identity, 65% coverage
- mutant phenotype: Specific phenotype on L-Histidine. This gene cluster also includes a permease of the nucleobase-cation-symport family (AO356_09625) that is important for histidine utilization but whose role is unclear.
PSPTO_5273 histidine ABC transporter, ATP-binding protein, putative from Pseudomonas syringae pv. tomato str. DC3000
61% identity, 65% coverage
- Data-Independent Acquisition Proteomics Unravels the Effects of Iron Ions on Coronatine Synthesis in Pseudomonas syringae pv. tomato DC3000
He, Frontiers in microbiology 2020 - “...iron ion fermentation culture conditions. Significant upregulation of the expression of the ATP-binding protein hisP, PSPTO_5273, PSPTO_5561, PSPTO_1258, PSPTO_4916, PSPTO_4551, PSPTO_3740, and PSPTO_3739 was detected during the fermentation process. The ATP-binding protein syrD of the ABC transporter was reported to play an important role in the...”
- Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function
Chen, Journal of bacteriology 2007 - “...the following DC3000 genes: PSPTO_0462, PSPTO_3060, PSPTO_4575, and PSPTO_5273. An internal fragment of each gene was amplified using the primers listed in...”
- “...PSPTO_0462 (AAO54006), PSPTO_3060 (AAO56549), PSPTO_4575 (AAO58021), and PSPTO_5273 (AAO58699) as well as ProV from E. coli (ProV_Ec; AAA24427) and OpuCA from...”
A0J47_RS18910 glycine betaine/L-proline ABC transporter ATP-binding protein from Photobacterium damselae subsp. damselae
44% identity, 93% coverage
SCO1621 glycine betaine transport ATP-binding protein from Streptomyces coelicolor A3(2)
56% identity, 70% coverage
- Pre-sporulation stages of Streptomyces differentiation: state-of-the-art and future perspectives
Yagüe, FEMS microbiology letters 2013 - “...SCO0132 SCO1433 SCO2737 SCO3876 SCO5188 SCO5973 SCO6720 SCO0163 SCO1504 SCO2763 SCO3886 SCO5275 SCO6010 SCO6742 SCO0166 SCO1621 SCO2767 SCO3934 SCO5277 SCO6047 SCO6814 SCO0255 SCO1671 SCO2952 SCO3947 SCO5280 SCO6193 SCO6981 SCO0322 SCO1707 SCO2969 SCO3958 SCO5339 SCO6259 SCO7008 SCO0491 SCO1719 SCO2975 SCO4075 SCO5383 SCO6295 SCO7051 SCO0493 SCO1742 SCO3005 SCO4116...”
- Uncovering genes with divergent mRNA-protein dynamics in Streptomyces coelicolor
Jayapal, PloS one 2008 - “...glycine degradation), a serine hydroxymethyltransferase (SCO5470, GlyA; serine-glycine interconversion) and a related glycine betaine transporter (SCO1621, OpuAA) all of which are required for generating one-carbon moieties to be used in several cellular processes like methyl transfer reactions and purine biosynthesis were negatively correlated with PC-1 (two...”
PBPRB1788 putative glycine betaine ABC transporter ATP-binding protein from Photobacterium profundum SS9
42% identity, 93% coverage
TC 3.A.1.12.6 / Q9KKE1 HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine from Rhizobium meliloti (Sinorhizobium meliloti) (see 2 papers)
SMc00670 HISTIDINE TRANSPORT ATP-BINDING ABC TRANSPORTER PROTEIN from Sinorhizobium meliloti 1021
58% identity, 65% coverage
LMRG_02114 glycine betaine/L-proline ABC transporter, ATP-binding protein from Listeria monocytogenes 10403S
48% identity, 83% coverage
- c-di-AMP modulates Listeria monocytogenes central metabolism to regulate growth, antibiotic resistance and osmoregulation
Whiteley, Molecular microbiology 2017 - “..., lmo2195 , LMRG_01637 ; relA , lmo1523 , LMRG_01447 ; gbuA , lmo1014 , LMRG_02114 ; pstA , lmo2692 , LMRG_02239 ; cbpB , lmo1009 , LMRG_02109 ; pycA , lmo1072 , LMRG_00534 ; citZ , lmo1567 , LMRG_01400 ; citC , lmo1566 , LMRG_01401...”
pRL120514 putative ATP-binding component of ABC transporter from Rhizobium leguminosarum bv. viciae 3841
46% identity, 83% coverage
NGR_c27420 glycine betaine/L-proline ABC transporter ATP-binding protein from Sinorhizobium fredii NGR234
NGR_c27420 putative glycine betaine/L-proline transport ATP binding subunit from Rhizobium sp. NGR234
57% identity, 65% coverage
- High-resolution transcriptomic analyses of Sinorhizobium sp. NGR234 bacteroids in determinate nodules of Vigna unguiculata and indeterminate nodules of Leucaena leucocephala
Li, PloS one 2013 - “...561055 56768 lipoprotein-releasing NGR_c10690-NGR_c10700 194328 90132 77128 lipopolysaccharide NGR_c00180,NGR_c09020-NGR_c09030 165342 6687 4478 glycine betaine/proline NGR_c27400- NGR_c27420 94250 2837 942 multiple sugar NGR_c33060- NGR_c33090; NGR_c30950-NGR_c30980 58455 1236 341 simple sugar NGR_c14510-NGR_c14540 97854 24356 19296 branched-chain amino acids NGR_c25150, NGR_c25170-NGR_c25200 9614353 1051230 1891296 peptides/nickel NGR_c24410-NGR_c24450 66370 25108 2195...”
- “...were induced in bacteroids (RPKM=19312 versus RPKM=524 in the free-living condition), while others ( NGR_c27400- NGR_c27420 ) were repressed (RPKM=942 versus RPKM=94250 in the free-living condition). Similar phenomena were observed for transporter proteins of simple and multiple sugars ( Table 3 ). Moreover, a number of...”
AO353_07790 ABC transporter for Carnitine, ATPase component from Pseudomonas fluorescens FW300-N2E3
43% identity, 95% coverage
- mutant phenotype: Specific phenotypes on Carnitine Hydrochloride. Note that both carnitine and choline are catabolized via glycine betaine and this is clustered with glycine betaine degradation genes. This ABC transporter might also transport choline or glycine betaine with a different SBP (AO353_07780).
TC 3.A.1.12.12 / Q9HTI8 Probable ATP-binding component of ABC transporter, component of The CbcWV/CbcX (choline)/CaiX (carnitine)/BetX (betaine) transporter with 3 binding receptors for distinct quaternary ammonium compounds. Only the ligand-bound receptor binds to the transporter with high affinity from Pseudomonas aeruginosa (see paper)
PA5376 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa PAO1
PA14_71000 putative lycine betaine/L-proline ABC transporter, ATP-binding subunit from Pseudomonas aeruginosa UCBPP-PA14
52% identity, 68% coverage
- substrates: Carnitine, Choline, Glycine betaine
- A Novel XRE-Type Regulator Mediates Phage Lytic Development and Multiple Host Metabolic Processes in Pseudomonas aeruginosa
Long, Microbiology spectrum 2022 - “...Fig.7A . The expression levels of potA , potB , potC , potD , and PA5376 were significantly downregulated in P8D, all of which belong to the ATP binding cassette (ABC) transporters in the transcriptome data. The SPD transport activity was determined by measuring [ 13...”
- In Vitro Activity of the Ultrabroad-Spectrum Beta-Lactamase Inhibitor QPX7728 in Combination with Multiple Beta-Lactam Antibiotics against Pseudomonas aeruginosa
Lomovskaya, Antimicrobial agents and chemotherapy 2021 - “...VIM-2; 32 64 32 16 8 PA5282 NF Up CTX-M-3 16 32 64 32 8 PA5376 FL Wt NDM-1 64 4 64 >64 >64 PA1068 NF Wt SPM-1 >64 4 64 >64 >64 PA5426 NF Up ND 32 32 64 2 32 PA5309 NF Up ND...”
- Differential requirements for processing and transport of short-chain versus long-chain O-acylcarnitines in Pseudomonas aeruginosa
Meadows, Microbiology (Reading, England) 2018 (secret) - Choline Catabolism in Burkholderia thailandensis Is Regulated by Multiple Glutamine Amidotransferase 1-Containing AraC Family Transcriptional Regulators
Nock, Journal of bacteriology 2016 - “...of catabolic genes, including gbcA-B, the dgc operon (PA5376, PA5377, dgcA, dgcB, PA5400, and PA5401), and the sarcosine oxidase genes, which together...”
- Characterization of the GbdR regulon in Pseudomonas aeruginosa
Hampel, Journal of bacteriology 2014 - “...in abundancec PA0843 PA0844 PA2761 PA2762 PA3236 PA5292 PA5376 PA5377 PA5378 PA5379 PA5396 PA5397 PA5398 PA5399 PA5401 PA5402 PA5410 PA5411 PA5415 PA5416 PA5417...”
- The ATP-binding cassette transporter Cbc (choline/betaine/carnitine) recruits multiple substrate-binding proteins with strong specificity for distinct quaternary ammonium compounds
Chen, Molecular microbiology 2010 - “...(1995) PA14 caiX PA14 PA5388 Wargo and Hogan (2009) PA5376:tn PA14 with transposon insertion in PA5376; Gm r Liberati et al. (2006) PA5377:tn PA14 with transposon insertion in PA5377; Gm r Liberati et al. (2006) PA5378:tn PA14 with transposon insertion in PA5378; Gm r Liberati et...”
- Roles of three transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa choline uptake for catabolism
Malek, Journal of bacteriology 2011 - “...only OpuC (PA14_13580 to PA14_13600) and CbcVWX (PA14_71000 to PA14_71030), but also three BCCTs, designated BetT1, BetT2, and BetT3 (PA14_70980, PA14_12990,...”
Pf6N2E2_4681 ABC transporter for Carnitine, ATPase component from Pseudomonas fluorescens FW300-N2E2
53% identity, 67% coverage
- mutant phenotype: Specific phenotype on Carnitine Hydrochloride. This ABC transporter might also take up choline or glycine-betaine, in conjunction with another SBP, Pf6N2E2_4683
AO356_13805 ABC transporter for Carnitine, ATPase component from Pseudomonas fluorescens FW300-N2C3
52% identity, 67% coverage
- mutant phenotype: Specific phenotype on Carnitine Hydrochloride. Likely to be a choline or glycine betaine transporter as well (with AO356_13855 as the SBP)
VIBHAR_06176 ATP-binding component of ABC transporter from Vibrio harveyi ATCC BAA-1116
42% identity, 97% coverage
PSPTO_0462 glycine/betaine/L-proline ABC transporter, ATP-binding subunit from Pseudomonas syringae pv. tomato str. DC3000
51% identity, 67% coverage
Psyr_4711 Glycine betaine/L-proline transport ATP-binding subunit from Pseudomonas syringae pv. syringae B728a
51% identity, 67% coverage
- Gene Expression Profiling in Viable but Nonculturable (VBNC) Cells of Pseudomonas syringae pv. syringae
Postnikova, Frontiers in microbiology 2015 - “...dependent transport system Polyamine metabolism and transport Psyr_4781 2.43 4Fe-4S ferredoxin QAC metabolism and transport Psyr_4711 2.49 Glycine betaine/L-proline transporter QAC metabolism and transport Nine up-regulated genes from the category Peptidoglycan/cell wall polymers (Supplementary Tables 1 , 3 ), including D-alanyl-D-alanine endopeptidase (Psyr_2414) and UDPdiphospho-muramoylpentapeptide beta-N-...”
- “...activity. One of the up-regulated genes in this category is a transporter for glycine betaine/L-proline (Psyr_4711). Glycine betaine acts as an osmoprotectant in plants, bacteria, and animals and its accumulation can reduce effect of stress and increase survival (Hanson et al., 1994 ). Induction of 4Fe-4S...”
- Genome-driven investigation of compatible solute biosynthesis pathways of Pseudomonas syringae pv. syringae and their contribution to water stress tolerance
Kurz, Applied and environmental microbiology 2010 - “...KURZ ET AL. (Psyr_4249), and CbcXWV (Psyr_4709 to Psyr_4711), responsible for the transport of the essential precursor choline, have already been identified by...”
C7CH17 Quaternary amine transport ATP-binding protein from Methylorubrum extorquens (strain DSM 6343 / CIP 106787 / DM4)
44% identity, 88% coverage
PP_0294 Choline / carnitine / glycine betaine ABC transporter, ATPase component from Pseudomonas putida KT2440
PP0294, PP_0294 glycine betaine/L-proline ABC transporter, ATP-binding subunit from Pseudomonas putida KT2440
PPUBIRD1_0323 choline ABC transporter ATP-binding protein from Pseudomonas putida BIRD-1
43% identity, 95% coverage
- mutant phenotype: Specific phenotype: utilization of Carnitine, Choline. Probably part of a glycine-betaine transporter as well (see PA5376 and TC 3.A.1.12.12)
- Gene expression reprogramming of Pseudomonas alloputida in response to arginine through the transcriptional regulator ArgR
Molina-Henares, Microbiology (Reading, England) 2024 - “...catabolism + + PP_4480 astA-II arginine catabolism + + PP_4486 argT arginine transport + + PP_0294 cbcV choline/betaine/carnitine transport + PP_0308 dipeptidase + PP_0310 dgcA dimethylglycine dehydrogenase + PP_0311_0312_0313 dgcB dimethylglycine dehydrogenase; flavoprotein subunits + PP_0315 gbcA glycine-betaine dioxygenase subunit + PP_0326 soxG sarcosine oxidase subunit...”
- UEG Week 2024 Poster Presentations
, United European gastroenterology journal 2024 - UEG Week 2023 Poster Presentations
, United European gastroenterology journal 2023 - Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing
Schmidt, Applied and environmental microbiology 2022 (secret) - 2-Aminoethylphosphonate utilization in Pseudomonas putida BIRD-1 is controlled by multiple master regulators
Murphy, Environmental microbiology 2022 - “...Low N upregulated 2.36 PPUBIRD1_1769 GlgX glycogen debranching enzyme NtrBC dependent, Low N upregulated 2.78 PPUBIRD1_0323 Glycine betaine/ l proline ABC transporter, ATPbinding subunit NtrBC dependent, Low N upregulated 2.58 PPUBIRD1_1785 NCS1 nucleoside transporter NtrBC dependent, Low N upregulated 2.44 PPUBIRD1_1182 LivG Leucine/isoleucine/valine ABC transporter, solutebinding...”
VPA1109 putative ATP-binding component of ABC transporter from Vibrio parahaemolyticus RIMD 2210633
42% identity, 97% coverage
WU75_10380 choline ABC transporter ATP-binding protein from Vibrio parahaemolyticus
42% identity, 97% coverage
- Identification of Antibacterial Components and Modes in the Methanol-Phase Extract from a Herbal Plant Potentilla kleiniana Wight et Arn
Tang, Foods (Basel, Switzerland) 2023 - “...in V. parahaemolyticus ATCC17802 ( p < 0.05). Of these, the DEGs ( proVXW , WU75_10380 , WU75_10390 , and WU75_10385 ), encoding a choline ABC transporter ATP-binding protein, a choline ABC transporter substrate-binding protein, and a choline ABC transporter permease subunit that are responsible for...”
- “...WU75_10390 proX 0.122 Glycine/betaine ABC transporter substrate-binding protein WU75_12775 oppC 0.133 Peptide ABC transporter permease WU75_10380 proV 0.138 ABC transporter ATP-binding protein WU75_09655 aotM 0.143 Amino acid ABC transporter permease WU75_09665 aotJ 0.144 Nickel transporter WU75_13090 yejA 0.151 Diguanylate cyclase WU75_12770 oppB 0.164 Oligopeptide transporter permease...”
TC 3.A.1.12.13 / A9CI32 ABC transporter, nucleotide binding/ATPase protein (Proline/glycine betaine), component of High affinity (2mμM) choline uptake porter. The choline binding receptor exhibits a venus fly trap mechanism of substrate binding. (ChoX binds acetyl choline and betaine with low affinity (80μM and 470μM, respectively) (Aktas et al., 2011) (most similar to 3.A.1.12.7) from Agrobacterium tumefaciens (strain C58 / ATCC 33970) (see 2 papers)
Atu2279 ABC transporter, nucleotide binding/ATPase protein (proline/glycine betaine) from Agrobacterium tumefaciens str. C58 (Cereon)
51% identity, 68% coverage
- substrates: Choline
- Agrobacterium tumefaciens Type IV and Type VI Secretion Systems Reside in Detergent-Resistant Membranes
Czolkoss, Frontiers in microbiology 2021 - “...FixG Nah atu1574 A9CIX0 Salicylate hydroxylase KefB atu1693 A9CIR7 Glutathione-regulated potassium-efflux system protein Atu2279 atu2279 A9CI32 ABC transporter, nucleotide binding/ATPase protein Atu2284 atu2284 A9CI31 Unknown Atu2550 atu2550 Q7CWT1 RND multidrug efflux membrane permease Atu2710 atu2710 Q7CWF5 Peptidase_M48 domain-containing protein Atu3507 atu3507 Q7CSM9 SH3b domain-containing protein Atu3546...”
- Agrobacterium tumefaciens Type IV and Type VI Secretion Systems Reside in Detergent-Resistant Membranes
Czolkoss, Frontiers in microbiology 2021 - “...fixation protein FixG Nah atu1574 A9CIX0 Salicylate hydroxylase KefB atu1693 A9CIR7 Glutathione-regulated potassium-efflux system protein Atu2279 atu2279 A9CI32 ABC transporter, nucleotide binding/ATPase protein Atu2284 atu2284 A9CI31 Unknown Atu2550 atu2550 Q7CWT1 RND multidrug efflux membrane permease Atu2710 atu2710 Q7CWF5 Peptidase_M48 domain-containing protein Atu3507 atu3507 Q7CSM9 SH3b domain-containing...”
- Choline uptake in Agrobacterium tumefaciens by the high-affinity ChoXWV transporter
Aktas, Journal of bacteriology 2011 - “...for a putative permease (Atu2280) and an ATPase (Atu2279) are encoded downstream of atu2281. Based on the functional analysis reported in this study, these...”
- “...Analysis Database (40), we identified atu2281, atu2280, and atu2279 encoding a putative choline ABC-transporter similar to the ChoXWV system from S. meliloti....”
SYNW1915 ABC transporter superfamily for glycine betaine/proline, ATP binding from Synechococcus sp. WH 8102
52% identity, 70% coverage
- Computational prediction of the osmoregulation network in Synechococcus sp. WH8102
Mao, BMC genomics 2010 - “...b0314 BetT SYNW0229 SYNW0229-0233 0.99 E. coli b4111 ProP SYNW2494 SYNW2494-2495 E. coli b2677 ProV SYNW1915 SYNW1915-1917 0.56 E. coli b2678 ProW SYNW1916 SYNW1915-1917 0.99 E. coli b2679 ProX SYNW1917 SYNW1915-1917 0.99 E. coli AB094497 GsmT SYNW1914 PCC7418 AB094498 SdmT SYNW1913 PCC7418 sll1566 GgpS SYNW1281 SYNW1279-1286...”
- “...mapped, which include the following: SYNW2169 (KtrA) does not have PFPF02080, the TrkA C-terminal domain; SYNW1915 (ProV) does not have PF00571, the CBS domain; SYNW2520 (SpsA) does not have PF00862, the sucrose synthase domain; SYNW0944 (idcC) does not have the PF03709, the Orn/Lys/Arg decarboxylase, N-terminal domain;...”
- Operon prediction by comparative genomics: an application to the Synechococcus sp. WH8102 genome
Chen, Nucleic acids research 2004 - “...(SYNW1283, (SYNW1340, (SYNW1415, (SYNW1797, (SYNW1857, (SYNW1915, (SYNW2393, (SYNW2438, (SYNW2479, (SYNW2485, Exactly found (SYNW0319, 0320, 0321, 0322)...”
TC 3.A.1.12.7 / Q92N35 ChoV, component of High affinity (3 μM) choline-specific uptake system from Rhizobium meliloti (Sinorhizobium meliloti) (see 2 papers)
SMc02739 PUTATIVE GLYCINE BETAINE TRANSPORT ATP-BINDING ABC TRANSPORTER PROTEIN from Sinorhizobium meliloti 1021
52% identity, 67% coverage
VVA0511 ABC-type proline/glycine betaine transport system, ATPase component from Vibrio vulnificus YJ016
54% identity, 63% coverage
OA04_25030 ATP-binding cassette domain-containing protein from Pectobacterium versatile
57% identity, 59% coverage
BAB1_1595 ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase from Brucella melitensis biovar Abortus 2308
BMEI0439 GLYCINE BETAINE/L-PROLINE TRANSPORT ATP-BINDING PROTEIN PROV from Brucella melitensis 16M
52% identity, 67% coverage
- Identification and characterization of a high-affinity choline uptake system of Brucella abortus
Herrmann, Journal of bacteriology 2013 - “...inner membrane protein (BAB1_1594) and an ATPase (BAB1_1595). Additionally, other important characteristics of this protein are the presence of an OpuAC...”
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...BOV_A0279 OSP Maltose import BP BMEII0945 BruAb20877 BRA0304 BCAN_B0305 82 OTCN Glycine betaine/L-proline import ABC BMEI0439, proV BruAb11568 BR1581 BOV_1526 BCAN_A1616 OTCN Glycine betaine/L-proline import IM BMEI0440, proW BruAb11567 BR1580 BOV_1525 BCAN_A1615 OTCN Glycine betaine/L-proline import BP BMEI0441, proX BruAb11566 BR1579 BOV_1524 BCAN_A1614 83 OTCN Choline...”
BOV_1526 putative glycine betaine/L-proline ABC transporter, ATP-binding protein from Brucella ovis ATCC 25840
52% identity, 67% coverage
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...BP BMEII0945 BruAb20877 BRA0304 BCAN_B0305 82 OTCN Glycine betaine/L-proline import ABC BMEI0439, proV BruAb11568 BR1581 BOV_1526 BCAN_A1616 OTCN Glycine betaine/L-proline import IM BMEI0440, proW BruAb11567 BR1580 BOV_1525 BCAN_A1615 OTCN Glycine betaine/L-proline import BP BMEI0441, proX BruAb11566 BR1579 BOV_1524 BCAN_A1614 83 OTCN Choline S S -dependent regulation...”
SMc04439 PUTATIVE GLYCINE BETAINE TRANSPORT ATP-BINDING ABC TRANSPORTER PROTEIN from Sinorhizobium meliloti 1021
55% identity, 57% coverage
BR1581 glycine betaine/L-proline ABC transporter, ATP-binding protein, putative from Brucella suis 1330
52% identity, 67% coverage
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...import BP BMEII0945 BruAb20877 BRA0304 BCAN_B0305 82 OTCN Glycine betaine/L-proline import ABC BMEI0439, proV BruAb11568 BR1581 BOV_1526 BCAN_A1616 OTCN Glycine betaine/L-proline import IM BMEI0440, proW BruAb11567 BR1580 BOV_1525 BCAN_A1615 OTCN Glycine betaine/L-proline import BP BMEI0441, proX BruAb11566 BR1579 BOV_1524 BCAN_A1614 83 OTCN Choline S S -dependent...”
HQ843_RS05790 choline ABC transporter ATP-binding protein from Martelella sp. NC20
49% identity, 70% coverage
- Genomic Analysis of Halotolerant Bacterial Strains Martelella soudanensis NC18T and NC20
Lee, Journal of microbiology and biotechnology 2022 - “...K01271 Xaa-Pro dipeptidase HQ775_RS07200 HQ843_RS23495 proV K02000 glycine betaine transport system ATP-binding protein HQ775_RS10910 HQ843_RS09775, HQ843_RS05790, HQ843_RS19795 proW K02001 glycine betaine transport system permease protein HQ775_RS24955, HQ775_RS10905 HQ843_RS05795, HQ843_RS19800 proX K02002 glycine betaine transport system substrate-binding protein HQ775_RS10900 HQ843_RS19805 TC.BCT K03451 betaine/carnitine transporter, BCCT family HQ775_RS23930...”
BB0146 glycine betaine, L-proline ABC transporter, ATP-binding protein (proV) from Borrelia burgdorferi B31
BB_0146 glycine betaine/L-proline ABC transporter ATP-binding protein from Borreliella burgdorferi B31
43% identity, 70% coverage
- Cyclic di-GMP modulates gene expression in Lyme disease spirochetes at the tick-mammal interface to promote spirochete survival during the blood meal and tick-to-mammal transmission
Caimano, Infection and immunity 2015 - “...bbi38 bbh13 bbj41 bb0373 bb0580 bb0694 bb0397 bb0442 bb0146 bb0116 bb0754 bb0170 bb0089 c-di-GMP Modulates Gene Expression in B. burgdorferi (14, 96) were...”
- Motility is crucial for the infectious life cycle of Borrelia burgdorferi
Sultan, Infection and immunity 2013 - “...flaB target gene is separated from adjacent fliD and bb0146 genes by 127 bp and 154 bp, respectively. The PflgBkan cassette containing two AgeI sites was...”
- Rrp1, a cyclic-di-GMP-producing response regulator, is an important regulator of Borrelia burgdorferi core cellular functions
Rogers, Molecular microbiology 2009 - “...Narasimhan et al . (2002) BB0140 acriflavine resistance protein ( acrB ) X 11.6 0.0136 BB0146 glycine betaine, L-proline ABC transporter, ATP-binding protein ( proV ) TP 80.1 0.0132 Narasimhan et al . (2002) BB0149 flagellar hook-associated protein 2 ( fliD ) F 7.87 0.0300 BB0151...”
- Complementation of a nonmotile flaB mutant of Borrelia burgdorferi by chromosomal integration of a plasmid containing a wild-type flaB allele
Sartakova, Journal of bacteriology 2001 - “...used for PCR. Primer 5 is located within proV (BB0146). Primer 4 is located in the intergenic region between proV and flaB (BB0147), 146 nucleotides downstream...”
- Distinction between Borrelia and Borreliella is more robustly supported by molecular and phenotypic characteristics than all other neighbouring prokaryotic genera: Response to Margos' et al. "The genus Borrelia reloaded" (PLoS ONE 13(12): e0208432)
Gupta, PloS one 2019 - “...BRE47 BB_0044 5 aa - - + Distinguishes two genera L-proline transport system ATP-binding protein BB_0146 1 aa - - + Distinguishes two genera Penicillin-binding protein BB_0136 1 aa - - + Distinguishes two genera Hypothetical protein Q7M131 BB_0125 1 aa + + - Distinguishes two...”
SPO2443 glycine betaine/L-proline ABC transporter ATP-binding protein from Ruegeria pomeroyi DSS-3
47% identity, 69% coverage
BCAN_A1616 ABC transporter related from Brucella canis ATCC 23365
45% identity, 81% coverage
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...BMEII0945 BruAb20877 BRA0304 BCAN_B0305 82 OTCN Glycine betaine/L-proline import ABC BMEI0439, proV BruAb11568 BR1581 BOV_1526 BCAN_A1616 OTCN Glycine betaine/L-proline import IM BMEI0440, proW BruAb11567 BR1580 BOV_1525 BCAN_A1615 OTCN Glycine betaine/L-proline import BP BMEI0441, proX BruAb11566 BR1579 BOV_1524 BCAN_A1614 83 OTCN Choline S S -dependent regulation of...”
HQ843_RS09775 choline ABC transporter ATP-binding protein from Martelella sp. NC20
50% identity, 72% coverage
- Genomic Analysis of Halotolerant Bacterial Strains Martelella soudanensis NC18T and NC20
Lee, Journal of microbiology and biotechnology 2022 - “...pepQ K01271 Xaa-Pro dipeptidase HQ775_RS07200 HQ843_RS23495 proV K02000 glycine betaine transport system ATP-binding protein HQ775_RS10910 HQ843_RS09775, HQ843_RS05790, HQ843_RS19795 proW K02001 glycine betaine transport system permease protein HQ775_RS24955, HQ775_RS10905 HQ843_RS05795, HQ843_RS19800 proX K02002 glycine betaine transport system substrate-binding protein HQ775_RS10900 HQ843_RS19805 TC.BCT K03451 betaine/carnitine transporter, BCCT family...”
SPO1549 glycine betaine/L-proline ABC transporter ATP-binding protein from Ruegeria pomeroyi DSS-3
50% identity, 68% coverage
- Bacterial transcriptional response to labile exometabolites from photosynthetic picoeukaryote Micromonas commoda
Ferrer-González, ISME communications 2023 - “...2.8 phosphonate ABC transporter, permease phnE -2 SPO1548 AAV94835.1 6.5 TMAO transporter, substrate-binding protein tmoX SPO1549 AAV94836.1 6.8 TMAO transporter, ATP-binding protein tmoW SPO1550 AAV94837.1 6.4 TMAO transporter, permease tmoV SPO2356 AAV95617.1 3.7 isethionate TRAP transporter, large permease iseM SPO2357* AAV95618.1 3.5 isethionate TRAP transporter, small...”
- Diel investments in metabolite production and consumption in a model microbial system
Uchimiya, The ISME journal 2022 - “...ABC transporter, periplasmic binding 38.0 [ 53 ] SPO1550 tmoV ABC transporter, permease protein 41.2 SPO1549 tmoW ABC transporter, ATP binding 43.1 Amine TMA SPO1551 tmm TMA monooxygenase 42.6 [ 25 ] Amino acid derivative Betaine SPO3186 opuD Glycine-betaine transporter 1.6 (n.s.) [ 25 ] Amino...”
TMOW_RUEPO / Q5LT65 Trimethylamine N-oxide transport system ATP-binding protein TmoW; TMAO transport system ATP-binding protein TmoW; EC 7.6.2.9 from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) (see paper)
50% identity, 68% coverage
- function: Part of the ABC transporter complex TmoXWV involved in trimethylamine N-oxide (TMAO) import (PubMed:24550299). Responsible for energy coupling to the transport system (Probable). Is specific for TMAO and essential for TMAO metabolism (PubMed:24550299).
catalytic activity: a quaternary ammonium(out) + ATP + H2O = a quaternary ammonium(in) + ADP + phosphate + H(+) (RHEA:11036)
subunit: The complex is probably composed of two ATP-binding proteins (TmoW), two transmembrane proteins (TmoV) and a solute-binding protein (TmoX).
disruption phenotype: The double mutant tmoXW shows significant reduced growth on TMAO as a sole nitrogen source (PubMed:24550299). Growth on trimethylamine (TMA), glycine betaine (GBT), choline and carnitine is unaffected (PubMed:24550299).
IEX10_RS05410 glycine betaine/L-proline ABC transporter ATP-binding protein from Amylibacter cionae
40% identity, 81% coverage
TMOW_PELUB / Q4FL37 Trimethylamine N-oxide transport system ATP-binding protein TmoW; TMAO transport system ATP-binding protein TmoW; EC 7.6.2.9 from Pelagibacter ubique (strain HTCC1062) (see paper)
45% identity, 70% coverage
- function: Part of the ABC transporter complex TmoXWV involved in trimethylamine N-oxide (TMAO) import (PubMed:35295296). Responsible for energy coupling to the transport system (Probable).
catalytic activity: a quaternary ammonium(out) + ATP + H2O = a quaternary ammonium(in) + ADP + phosphate + H(+) (RHEA:11036)
subunit: The complex is probably composed of two ATP-binding proteins (TmoW), two transmembrane proteins (TmoV) and a solute-binding protein (TmoX).
SPO1132 glycine betaine/L-proline ABC transporter ATP-binding protein from Ruegeria pomeroyi DSS-3
43% identity, 71% coverage
- Transcriptional changes underlying elemental stoichiometry shifts in a marine heterotrophic bacterium
Chan, Frontiers in microbiology 2012 - “...transcriptional regulator (carbon catabolite repression domain-containing) 14.5 SPO0970 Aquaporin Z ( apqZ ) 3.1 1.3 SPO1132 Glycine betaine/proline ABC transporter, ATP-binding protein 3.1 3.0 SPO1156 Imidazole glycerol phosphate synthase subunit ( hisF ) 3.0 3.5 SPO1463 TRAP dicarboxylate transporter, DctM subunit 8.3 SPO1645 Oligopeptide/dipeptide ABC transporter,...”
- “...F protein 3.2 SPO1063 Hypothetical protein 1.2 3.3 SPO1092 LysR family transcriptional regulator 3.0 1.8 SPO1132 Glycine betaine/proline ABC transporter, ATP-binding protein 3.1 3.0 SPO1138 AsnC family transcriptional regulator 6.2 4.7 SPO1156 hisF Imidazole glycerol phosphate synthase subunit 3.0 3.5 SPO1337 Hypothetical protein 3.9 SPO1446 Cyclase,...”
MED193_07698 glycine betaine/proline ABC transporter, ATP-binding protein from Roseobacter sp. MED193
42% identity, 74% coverage
- Bacterial catabolism of membrane phospholipids links marine biogeochemical cycles
Westermann, Science advances 2023 - “...BetA: choline dehydrogenase (MED193_21686); ChoW: ABC-type transporter, permease component (MED193_07693); ChoV: ABC-type transporter, ATP-binding component (MED193_07698); ChoX: ABC-type transporter, betaine/carnitine/choline binding protein (MED193_07703); RpiR: RpiR regulator (MED193_10021); GGAH: -glutamylglycine amidohydrolase (MED193_10026), EtoV: TRAP transporter, small permease component (MED193_10031); EtoW: TRAP transporter, large permease component (MED193_10036); EtoX:...”
CAC2850 Proline/glycine betaine ABC-type transport system, ATPase component from Clostridium acetobutylicum ATCC 824
34% identity, 91% coverage
Cbei_2871 glycine betaine/L-proline ABC transporter, ATPase subunit from Clostridium beijerincki NCIMB 8052
37% identity, 88% coverage
CBO1636 glycine betaine/carnitine/choline transport ATP-binding protein from Clostridium botulinum A str. ATCC 3502
35% identity, 96% coverage
CLIBASIA_01125 ATP-binding cassette domain-containing protein from Candidatus Liberibacter asiaticus str. psy62
44% identity, 67% coverage
- Molecular signatures between citrus and Candidatus Liberibacter asiaticus
Hu, PLoS pathogens 2021 - “...to synthesize PC. However, C Las encodes a predicted ABC transporter system for choline ( CLIBASIA_01125 ) and a phosphatidylcholine synthase ( CLIBASIA_02325 ) [ 75 ], suggesting that it is capable of utilizing extracellular choline. 4.2. Secretion-related genes C Las lacks type III and type...”
- The ABC transporters in Candidatus Liberibacter asiaticus
Li, Proteins 2012 - “...8e 68 52 CLIBASIA_02235 PF00528 (CL0404) CLIBASIA_02240 (PP) Thiamine (vitamin B 1 ) III(i) OTCN CLIBASIA_01125 ChoV S. meliloti 1e 114 60 CLIBASIA_01130 PF00528 (CL0404) CLIBASIA_01135 (PP) Choline (vitamin B p ) III(i) OTCN CLIBASIA_02415 NrtD S. elongatus 5e 42 39 CLIBASIA_02420 PF00528 (CL0404) N/A Possible...”
- “...rather than the annotated glycine-betaine substrate. Given the close relationship of the Ca. L. asiaticus CLIBASIA_01125 (NBD) component to that in S. meliloti as well as the similar operon arrangement (ChoX-ChoW-ChoV), we annotate these components as a Cho system. The highly similar TMD (ChoW, identity: 62%,...”
CD0900 glycine betaine/carnitine/choline ABC transporter, ATP-binding protein from Clostridium difficile 630
34% identity, 91% coverage
CPE0557 probable glycine betaine/carnitine/choline ABC transporter from Clostridium perfringens str. 13
36% identity, 87% coverage
SA2237 glycine betaine/carnitine/choline ABC transporter opuCA from Staphylococcus aureus subsp. aureus N315
SAV2448 glycine betaine/carnitine/choline ABC transporter from Staphylococcus aureus subsp. aureus Mu50
34% identity, 85% coverage
NWMN_2347 glycine betaine/L-proline transport ATP-binding subunit from Staphylococcus aureus subsp. aureus str. Newman
SACOL2453 amino acid ABC transporter, ATP-binding protein from Staphylococcus aureus subsp. aureus COL
34% identity, 86% coverage
- CodY in Staphylococcus aureus: a regulatory link between metabolism and virulence gene expression
Pohl, Journal of bacteriology 2009 - “...NWMN_1960 NWMN_1961 NWMN_1962 NWMN_1963 NWMN_1964 NWMN_1965 NWMN_1966 NWMN_2347 NWMN_2500 NWMN_2501 NWMN_2571 NWMN_2572 Description CodY IN S. AUREUS VOL. 191,...”
- Effect of a glucose impulse on the CcpA regulon in Staphylococcus aureus
Seidl, BMC microbiology 2009 - “...1.2 SA2236 NWMN_2346 opuCB probable glycine betaine/carnitine/choline ABC transporter (membrane part) OpuCB 1.9 1.1 *SA2237 NWMN_2347 opuCA glycine betaine/carnitine/choline ABC transporter (ATP-binding) OpuCA 2.6 1.0 SA2239 NWNM_2349 similar to amino acid transporter 2.2 1.1 SA2443 NWMN_2549 similar to accessory secretory protein Asp3 2.0 1.2 SA2444 NWMN_2550...”
- Transcriptomic and Metabolomic Analysis of a Fusidic Acid-Selected fusA Mutant of Staphylococcus aureus
Gupta, Antibiotics (Basel, Switzerland) 2022 - “...(SAV0727, SAV1380, and SAV1381) were upregulated in SH10001st-2, 8 other genes in this category (SACOL1476, SACOL2453, SAR2503, SAV0722, SAS0283, SAV2412, opuCA , and proP ) were downregulated ( Tables S1 and S2 ). At the same time, there were more amino acid biosynthesis genes downregulated (...”
- The second messenger c-di-AMP inhibits the osmolyte uptake system OpuC in Staphylococcus aureus
Schuster, Science signaling 2016 - “...c-di-AMP and the His-MBP-SACOL2453 fusion protein (fig. S1). SACOL2453 corresponds to protein SAUSA300_2393 in S. aureus strain FPR3757 and is annotated as...”
- “...ra81 3 A B O C C SA SA M BP O SACOL2453 (OpuCA) SACOL0460 (GuaB) SACOL0762 SACOL0921 SACOL1013 (MgtE) SACOL1621 (CcpN) SACOL1752 L2 45 3 SA L04 (Op 60 uC C O A...”
- Mode-of-action studies of the novel bisquaternary bisnaphthalimide MT02 against Staphylococcus aureus
Menzel, Antimicrobial agents and chemotherapy 2011 - “...SACOL1193 SACOL1195 SACOL1196 SACOL1319 SACOL2450 SACOL2452 SACOL2453 SACOL2475 Nucleotide metabolism; upregulated ORFs SACOL0791 Annotation Fold change dnaA...”
- A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome
Becher, PloS one 2009 - “...in the transport of amino acids or oligopeptides [from left to right: SACOL2451, SACOL2309, SACOL2452, SACOL2453, SACOL0993 (OppD), SACOL0992 (OppC), SACOL0994 (OppF), SACOL0995, SACOL1963, SACOL2450, SACOL0991 (OppB)], CPS (capsular polysaccharide) biosynthesis [SACOL0151 (Cap5P), SACOL0138 (Cap5C), SACOL0143 (Cap5H), SACOL0137 (Cap5B), SACOL0136 (Cap5A), SACOL0142 (Cap5G), SACOL0150 (Cap5O), SACOL0145...”
B4602_RS12935 betaine/proline/choline family ABC transporter ATP-binding protein from Staphylococcus aureus
34% identity, 86% coverage
Theba_2466 ABC transporter ATP-binding protein from Mesotoga prima MesG1.Ag.4.2
37% identity, 86% coverage
SAUSA300_2393 glycine betaine/carnitine/choline ABC transporter ATP-binding protein from Staphylococcus aureus subsp. aureus USA300_FPR3757
34% identity, 86% coverage
MW2372 glycine betaine/carnitine/choline ABC transporter opuCA from Staphylococcus aureus subsp. aureus MW2
34% identity, 85% coverage
OG1RF_10595 betaine/proline/choline family ABC transporter ATP-binding protein from Enterococcus faecalis OG1RF
EF0865 glycine betaine/carnitine/choline transporter, ATP-binding protein from Enterococcus faecalis V583
36% identity, 92% coverage
- ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis
Zheng, BMC microbiology 2020 - “...sbcC ) exonuclease SbcC 1.253 1.218 AEA95129.1 ( OG1RF_12442 ) FMN reductase 1.255 AEA93282.1 ( OG1RF_10595, opuAA ) glycine betaine/L-proline ABC superfamily ATP binding cassette transporter, ABC protein 1.253 AEA93239.1 ( OG1RF_10552, rplY ) 50S ribosomal protein L25 1.246 AEA93267.1 ( OG1RF_10580 ) PemK family transcriptional...”
- Transcriptomic and functional analysis of NaCl-induced stress in Enterococcus faecalis
Solheim, PloS one 2014 - “...binding proteins EF0864 0,29 1,37 1,34 Glycine betaine/carnitine/choline transporter, ATP-binding protein Transport and binding proteins EF0865 0,17 1,38 1,07 Glycine betaine/L-proline ABC transporter,ATP-binding subunit Transport and binding proteins EF2641 0,21 3,42 2,92 Glycine betaine/L-proline ABC transporter, glycine betaine/L-proline-binding/permease protein Transport and binding proteins EF2642 0,69 4,99...”
- Growth rate-dependent control in Enterococcus faecalis: effects on the transcriptome and proteome, and strong regulation of lactate dehydrogenase
Mehmeti, Applied and environmental microbiology 2012 - “...and binding proteinsa EF1741 EF3289 EF1050 EF0233 EF0865 Catabolite control protein A DNA-binding response regulator DNA-binding response regulator DNA direct...”
- “...transduction; EF0233 is involved in signal transcription; and EF0865 is involved in transport and binding. tween the results obtained with microarray and...”
G5T25_09120 betaine/proline/choline family ABC transporter ATP-binding protein from Enterococcus faecalis
36% identity, 92% coverage
NPW32_RS04150 methionine ABC transporter ATP-binding protein from Staphylococcus epidermidis
46% identity, 56% coverage
OPUBA_BACSU / Q45460 Choline transport ATP-binding protein OpuBA from Bacillus subtilis (strain 168) (see 2 papers)
TC 3.A.1.12.3 / Q45460 OPBA aka OpuBA aka PROV, component of Choline porter from Bacillus subtilis (see 3 papers)
BSU33730 choline ABC transporter (ATP-binding protein) from Bacillus subtilis subsp. subtilis str. 168
36% identity, 87% coverage
- function: Involved in a high affinity multicomponent binding-protein- dependent transport system for choline. Probably responsible for energy coupling to the transport system.
- substrates: Choline
- In Silico Safety Assessment of <i>Bacillus</i> Isolated from Polish Bee Pollen and Bee Bread as Novel Probiotic Candidates
Bin, International journal of molecular sciences 2024 - “...Q45462 opuBB Choline transport system permease protein OpuBB Q45461 opuBA Choline transport ATP-binding protein OpuBA Q45460 opuCD Glycine betaine/carnitine/choline transport system permease protein OpuCD O34742 opuCC Glycine betaine/carnitine/choline-binding protein OpuCC O32243 opuCB Glycine betaine/carnitine/choline transport system permease protein OpuCB O34878 opuCA Glycine betaine/carnitine/choline transport ATP-binding protein...”
- Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function
Chen, Journal of bacteriology 2007 - “...pv. tomato PSPTO_4575), AAA60492 (E. coli YehX), Q45460 (B. subtilis OpuBA), O34992 (B. subtilis OpuCA), CAC99506 (L. monocytogenes OpuCA), CAC46836 (S....”
- A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century
Bremer, Microbial biotechnology 2023 - “...manganese ABC transporter (ATPbinding protein) BSU38810 msmX yxkG multiple sugar (maltodextrins, galactans) transporter ATPbinding protein BSU33730 opuBA proV choline ABC transporter (ATPbinding protein) BSU33830 opuCA yvbE glycine betaine/carnitine/choline/choline ABC transporter (ATPbinding protein) BSU35700 tagH _ ATPbinding teichoic acid precursor transporter component BSU13220 thiW ykoD thiamine/HMP ABC...”
- Secondary structural entropy in RNA switch (Riboswitch) identification
Manzourolajdad, BMC bioinformatics 2015 - “...nt 3464551 3464750 reverse BSU33780 sdpI -1784 -40.60 - 0.3100 125.1740036 - 0.4450 1399 opuBA BSU33730 0.8446103930 200 nt 3684271 3684470 reverse BSU35780 lytD -456 -43.90 - 0.3450 125.0149994 - 0.3500 3399 tagD BSU35740 0.8443253636 200 nt 2770775 2770974 reverse BSU27160 cypB -3016 -41.20 - 0.3550...”
CBU_0178 ABC transporter, ATP-binding protein from Coxiella burnetii RSA 493
45% identity, 58% coverage
A21D_01627 betaine/proline/choline family ABC transporter ATP-binding protein from Virgibacillus dokdonensis
35% identity, 89% coverage
lp_1607 glycine betaine/carnitine/choline ABC transporter, ATP-binding protein from Lactobacillus plantarum WCFS1
35% identity, 88% coverage
lp_1607 betaine/proline/choline family ABC transporter ATP-binding protein from Lactiplantibacillus plantarum WCFS1
35% identity, 88% coverage
SERP0489 ABC transporter, ATP-binding protein from Staphylococcus epidermidis RP62A
45% identity, 56% coverage
NPW32_RS02740 betaine/proline/choline family ABC transporter ATP-binding protein from Staphylococcus epidermidis
36% identity, 87% coverage
OPUCA_BACSU / O34992 Glycine betaine/carnitine/choline transport ATP-binding protein OpuCA from Bacillus subtilis (strain 168) (see 4 papers)
TC 3.A.1.12.4 / O34992 OPCA aka OpuCA, component of Uptake system for choline, L-carnitine, D-carnitine, glycine betaine, proline betaine, crotonobetaine, γ-butyrobetaine, dimethylsulfonioacetate, dimethylsulfoniopropionate, ectoine and choline-O-sulfate from Bacillus subtilis (see 2 papers)
BSU33830 glycine betaine/carnitine/choline/choline sulfate ABC transporter (ATP-binding protein) from Bacillus subtilis subsp. subtilis str. 168
35% identity, 87% coverage
- function: Involved in a high affinity multicomponent binding-protein- dependent transport system for glycine betaine, carnitine and choline; probably responsible for energy coupling to the transport system.
subunit: The complex is composed of two ATP-binding proteins (OpuCA), two transmembrane proteins (OpuCB and OpuCD) and a solute-binding protein (OpuCC). - substrates: Carnitine, Choline, Choline-O-sulfate, Crotonobetaine, Dimethylsulfonioacetate, Dimethylsulfoniopropionate, Ectoine, Gamma-butyrobetaine, Glycine betaine, Proline betaine
- In Silico Safety Assessment of <i>Bacillus</i> Isolated from Polish Bee Pollen and Bee Bread as Novel Probiotic Candidates
Bin, International journal of molecular sciences 2024 - “...Glycine betaine/carnitine/choline transport system permease protein OpuCB O34878 opuCA Glycine betaine/carnitine/choline transport ATP-binding protein OpuCA O34992 opuE Osmoregulated proline transporter OpuE O06493 opuAA Glycine betaine transport ATP-binding protein OpuAA P46920 opuAB Glycine betaine transport system permease protein OpuAB P46921 opuAC Glycine betaine-binding protein OpuAC P46922 Adhesion...”
- A decade of research on the second messenger c-di-AMP
Yin, FEMS microbiology reviews 2020 - “...1.2 M NAa NA AAW38543 CAC99506 CAB15388 CAD45880 A0A160MQL0 Q7AP65 O34992 Q8E7C0 Schuster et al. 2016 Huynh et al. 2016 Gundlach et al. 2019 Devaux et al. 2018b...”
- A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study
Albaum, Proteome science 2011 - “...>0.99 0.000002 892 P27206 <0.000001 0.000004 0.000028 <0.000001 2232 P37871 <0.000001 0.399160 0.901907 <0.000001 1938 O34992 <0.000001 0.085647 <0.000001 <0.000001 1215 P37476 <0.000001 >0.99 <0.000001 <0.000001 3203 Q04747 <0.000001 <0.000001 0.000199 <0.000001 3460 O31709 <0.000001 >0.99 >0.99 0.000020 295 P35149 <0.000001 0.000169 >0.99 0.000693 751 P08065...”
- “...0.414572 <0.000001 2053 P27206 <0.000001 0.000004 0.000028 <0.000001 2232 P37871 <0.000001 0.399160 0.901907 <0.000001 1938 O34992 <0.000001 0.085647 <0.000001 <0.000001 1215 P37476 <0.000001 >0.99 <0.000001 <0.000001 3203 Q04747 <0.000001 <0.000001 0.000199 <0.000001 3460 P08750 <0.000001 >0.99 <0.000001 <0.000001 1109 P33166 <0.000001 <0.000001 0.067524 <0.000001 1605 P08065...”
- Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function
Chen, Journal of bacteriology 2007 - “...AAA24427) and OpuCA from B. subtilis (OpuCA_Bs; O34992) for comparison. Conserved nucleotide-binding protein motifs, including the Walker A (or P-loop), Q-loop,...”
- “...AAA60492 (E. coli YehX), Q45460 (B. subtilis OpuBA), O34992 (B. subtilis OpuCA), CAC99506 (L. monocytogenes OpuCA), CAC46836 (S. meliloti Prb), CAC48813 (S....”
- A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century
Bremer, Microbial biotechnology 2023 - “...multiple sugar (maltodextrins, galactans) transporter ATPbinding protein BSU33730 opuBA proV choline ABC transporter (ATPbinding protein) BSU33830 opuCA yvbE glycine betaine/carnitine/choline/choline ABC transporter (ATPbinding protein) BSU35700 tagH _ ATPbinding teichoic acid precursor transporter component BSU13220 thiW ykoD thiamine/HMP ABC transporter (ATPbinding subunit) BSU03140 tmrB _ ATPbinding tunicamycin...”
LSA1870 Putative glycine betaine/carnitine/choline ABC transporter, ATP-binding subunit from Lactobacillus sakei subsp. sakei 23K
46% identity, 55% coverage
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...membrane-spanning subunit -2.1 -2.0 -1.9 LSA1696 lsa1696 Putative glycine/betaine/carnitine ABC transporter, ATP-binding subunit -1.6 -0.9 LSA1870 lsa1870 Putative glycine betaine/carnitine/choline ABC transporter, ATP-binding subunit -0.6 -0.6 Protein modification LSA0865 lsa0865 Putative protein methionine sulfoxide reductase -0.6 LSA0866 msrA Protein methionine sulfoxide reductase -0.7 LSA0934 lplA Lipoate-protein...”
USA300HOU_0847 ABC superfamily ATP binding cassette transporter, ABC protein from Staphylococcus aureus subsp. aureus USA300_TCH1516
Q2FZZ2 Methionine import ATP-binding protein MetN 2 from Staphylococcus aureus (strain NCTC 8325 / PS 47)
SAUSA300_0796 ABC transporter, ATP-binding protein from Staphylococcus aureus subsp. aureus USA300_FPR3757
SACOL0882 ABC transporter, ATP-binding protein from Staphylococcus aureus subsp. aureus COL
45% identity, 56% coverage
- Exploring the transcriptome of Staphylococcus aureus in its natural niche
Chaves-Moreno, Scientific reports 2016 - “...the strong upregulation of two L-methionine ABC-transport systems ( met N1P1Q1, SACOL0504-0506 and met N2P2Q2 USA300HOU_0847 0849) previously observed in SNM 22 24 did not occur in vivo ( Fig. 3F ), indicating the strong upregulation of methionine biosynthetic genes and the respective transport systems to...”
- Antimicrobial Activity and Mechanism of Action of Dracocephalum moldavica L. Extracts Against Clinical Isolates of Staphylococcus aureus
Yu, Frontiers in microbiology 2019 - “...Glycerol phosphate lipoteichoic acid synthase LtaS1 18.68 0.000693 Q932F7 MSCRAMM family adhesin SdrE 14.6 0.002377 Q2FZZ2 Methionine ABC transporter ATP-binding MetN2 11.26 0.001239 Q2FWG4 Membrane insertion OxaA 10.54 0.000175 Q2G0Q9 Chaperonin (heat shock 33) HslO 10.52 0.000740 Q2G273 Urease accessory UreG 9.51 0.001393 Q2FYG5 Ubiquinone menaquinone...”
- Novel Methicillin-Resistant Staphylococcus aureus CC8 Clone Identified in a Hospital Setting in Armenia
Jamrozy, Frontiers in microbiology 2019 - “...and the insertion site was located downstream of an ABC-transporter operon (locus ID in USA300_FPR3757: SAUSA300_0796 to SAUSA300_0798). This corresponds to the insertion site of SaPI5 found in the USA300_FPR3757 reference genome, although the two SaPI elements shared low sequence similarity (98% sequence identity and 39%...”
- The ClpXP protease is dispensable for degradation of unfolded proteins in Staphylococcus aureus
Stahlhut, Scientific reports 2017 - “...3,5 6,6E-22 ilvB acetolactate synthase, large subunit 3,5 4,7E-20 comK competence transcription factor 3,4 2,5E-14 SAUSA300_0796 ABC transporter, ATP-binding protein 3,4 2,41E-15 SAUSA300_1029 iron transport associated domain protein 3,4 3,82E-14 SAUSA300_2417 putative transporter 3,4 2,2E-33 sarS staphylococcal accessory regulator 3,3 3,13E-09 asd aspartate semialdehyde dehydrogenase 3,3...”
- Cigarette Smoke Extract-Exposed Methicillin-Resistant Staphylococcus aureus Regulates Leukocyte Function for Pulmonary Persistence
Kulkarni, American journal of respiratory cell and molecular biology 2016 - “...SAUSA300_1726 SAUSA300_0846 SAUSA300_2537 SAUSA300_2436 SAUSA300_0796 SAUSA300_0207 SAUSA300_2389 SAUSA300_0360 SAUSA300_2609 SAUSA300_0557 SAUSA300_1814...”
- Virulence and Metabolism
Richardson, Microbiology spectrum 2019 (secret) - A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus
Chen, Molecular microbiology 2009 - “...6.3 -3.0 + ABC transporter SACOL0708 2.8 DAK2 domain protein SACOL0709 3.0 Conserved hypothetical protein SACOL0882 2.9 ABC transporter SACOL0884 2.1 -2.3 ABC transporter SACOL1476 13.8 Amino acid permiase SACOL1661 3.8 - Acetyl-CoA carboxylase, accC homologue SACOL1847 3.8 Conserved hypothetical protein SACOL1849 2.3 - Conserved hypothetical...”
GL331_01520 osmoprotectant ABC transporter ATP-binding protein OpuCA from Bacillus velezensis
35% identity, 87% coverage
SSP0451 ABC-type proline glycine betaine transport system ATPase component from Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305
DV527_RS02405 betaine/proline/choline family ABC transporter ATP-binding protein from Staphylococcus saprophyticus
36% identity, 87% coverage
GBAA2279 glycine betaine/L-proline ABC transporter, ATP-binding protein from Bacillus anthracis str. 'Ames Ancestor'
45% identity, 56% coverage
A9WPC5 Glycine betaine transport ATP-binding protein from Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)
41% identity, 66% coverage
G8O30_05615 ABC transporter ATP-binding protein from Mangrovibacillus cuniculi
43% identity, 56% coverage
- Insights Into the Cultivable Bacterial Fraction of Sediments From the Red Sea Mangroves and Physiological, Chemotaxonomic, and Genomic Characterization of Mangrovibacillus cuniculi gen. nov., sp. nov., a Novel Member of the Bacillaceae Family
Sefrji, Frontiers in microbiology 2022 - “...G8O30_05805, G8O30_05810, G8O30_05815), ectoine ( lysC , G8O30_09360), and ABC transporters of glycine betaine OpuABC (G8O30_05615, G8O30_05620, G8O30_05625), that suggest its ability to accumulate compatible solutes (e.g., ectoine, glycine betaine, and proline) to withstand the salt and osmotic stresses ( Oren, 2011 ). Beside these adaptations,...”
SAR0870 ABC transporter ATP-binding protein from Staphylococcus aureus subsp. aureus MRSA252
45% identity, 56% coverage
- Response of Staphylococcus aureus to subinhibitory concentrations of a sequence-selective, DNA minor groove cross-linking pyrrolobenzodiazepine dimer
Doyle, The Journal of antimicrobial chemotherapy 2009 - “...1 h. A high proportion of down-regulated genes encoded membrane proteins (e.g., SAR0182, yukA , SAR0870, opuD2) or proteins required for peptidoglycan synthesis, such as femA and murF ( Table 3 ). These changes had little or no impact on cell wall integrity or metabolism: overnight...”
- “...SAR0182 0.42 Putative membrane protein yukA 0.39 Putative membrane protein sucC 0.38 CoA synthetase protein SAR0870 0.41 ABC transporter ATP-binding protein SAR2558 0.41 Conserved hypothetical protein opuD2 0.42 Glycine betaine transporter 2 SAR0280 0.40 Putative membrane protein SAR1343 0.44 Amino acid permease COLB0262 0.41 Predicted CDS...”
BMMGA3_01135 ABC transporter ATP-binding protein from Bacillus methanolicus MGA3
46% identity, 56% coverage
SA0769 hypothetical protein from Staphylococcus aureus subsp. aureus N315
45% identity, 56% coverage
- Small-Molecule Antibiotics Inhibiting tRNA-Regulated Gene Expression Is a Viable Strategy for Targeting Gram-Positive Bacteria
Väre, Antimicrobial agents and chemotherapy 2020 - “...untreated and presented as a column graph). (C) Heatmap of some other 5UTR regulated genes: SA0769 and SA0011 are regulated by SAM riboswitches, SA1173 is a sigma B regulated CDS, SA0373 is regulated by a purine riboswitch, and SA1589 is a trans -encoded sRNA. (D to...”
- Characterization of RNA Helicase CshA and Its Role in Protecting mRNAs and Small RNAs of Staphylococcus aureus Strain Newman
Kim, Infection and immunity 2016 - “...sa0435 sa0468, ftsH sa0574, sarA sa0574, sarA saS023, sa0769 sa0892, sa0891 uvrC, sdhC codY, rpsB sa1590, ribD sa0372, xprT sa0543, sa0544 sa0601, sa0600 ssaA...”
- Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism
Bohn, Nucleic acids research 2010 - “...from premature transcriptional termination associated with riboswitch activities. RsaOM corresponds to the sequence upstream of SA0769 encoding a d -methionine transport system ATP-binding protein. Probing for this region revealed several small transcripts more expressed in the high salt stress condition ( Supplementary Data S4 ). The...”
- Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
Anderson, FEMS immunology and medical microbiology 2010 - “...SA0753 hypothetical protein sa_c8928s7841_a_at * 4.0 2.5 ND SA0755 hypothetical protein sa_c8200s7177_a_at 3.8 2.5 2.5 SA0769 hypothetical protein sa_c8226s7201_a_at 4.6 2.5 2.5 SA0776 hypothetical protein sa_c8228s7205_a_at * 4.1 2.5 2.5 SA0777 hypothetical protein sa_c8262s7237_a_at * 4.4 2.5 ND SA0790 hypothetical protein sa_c8288s7265_a_at 4.1 2.5 2.5 SA0800...”
- Effect of a glucose impulse on the CcpA regulon in Staphylococcus aureus
Seidl, BMC microbiology 2009 - “...SA0374 NWNM_0379 pbuX xanthine permease 7.2 1.1 *SA0655 NWNM_0669 fruA fructose specific permease 2.4 1.3 SA0769 NWNM_0780 D-methionine transport system ATP-binding protein 2.4 0.8 SA0770 NWNM_0781 D-methionine transport system permease 2.4 1.0 SA1270 NWNM_1347 similar to amino acid permease 2.0 1.1 SA2053 NWNM_2158 glucose uptake protein...”
- Microarray analysis of toxicogenomic effects of ortho-phenylphenol in Staphylococcus aureus
Jang, BMC genomics 2008 - “...amino acid ABC transporter, permease protein, glutamine transport system Inorganic ion transport and metabolism sa_c5431s4700_a_at SA0769 2.32E-06 -5.4 probable amino acid ABC transporter, ATP-binding protein Inorganic ion transport and metabolism sa_c8512s7471_a_at SA0770 1.42E-05 -6.3 permease protein of ABC transporter system Inorganic ion transport and metabolism sa_c8518s7475_a_at...”
- Overexpression of genes of the cell wall stimulon in clinical isolates of Staphylococcus aureus exhibiting vancomycin-intermediate- S. aureus-type resistance to vancomycin
McAleese, Journal of bacteriology 2006 - “...SA0217 SA0420 SA0421 SA0566 SA0587 SA0588 SA0589 SA0690 SA0769 SA0770 SA0927 SA0928 SA1016 SA2228 SA0270 SA0295 SA1777 SA1852 SA2097 SA2120 SA2194 SA2303 SA0077...”
DRA0137, DR_A0137 amino acid ABC transporter, ATP-binding protein from Deinococcus radiodurans R1
41% identity, 70% coverage
Atu0197 ABC transporter, nucleotide binding/ATPase protein (proline/glycine/betaine) from Agrobacterium tumefaciens str. C58 (Cereon)
41% identity, 76% coverage
TC 3.A.1.12.11 / Q8Q041 Glycine betaine transporter, ATP-binding protein, component of The glycine betaine uptake porter, GbpABCD from Methanosarcina mazei (see paper)
35% identity, 90% coverage
- substrates: Glycine betaine
BC2232 Glycine betaine transport ATP-binding protein from Bacillus cereus ATCC 14579
43% identity, 58% coverage
SERP2031 amino acid ABC transporter, ATP-binding protein from Staphylococcus epidermidis RP62A
34% identity, 84% coverage
PS417_27115 putrescine ABC transporter, ATPase component from Pseudomonas simiae WCS417
43% identity, 60% coverage
- mutant phenotype: Specific phenotype: utilization of Putrescine Dihydrochloride
A21D_03468 ABC transporter ATP-binding protein from Virgibacillus dokdonensis
38% identity, 71% coverage
PP0868, PP_0868 glycine betaine/carnitine/choline ABC transporter, ATP-binding protein, putative from Pseudomonas putida KT2440
36% identity, 90% coverage
- UEG Week 2024 Poster Presentations
, United European gastroenterology journal 2024 - UEG Week 2023 Poster Presentations
, United European gastroenterology journal 2023 - Global Transcriptional Responses to Osmotic, Oxidative, and Imipenem Stress Conditions in Pseudomonas putida
Bojanovič, Applied and environmental microbiology 2017 - “...function and locus tag Glycine betaine uptake PP_0868 PP_0869 PP_0870 PP_0871 PP_2914 PP_3957 PP_0229 PP_1944 PP_0989 PP_5193 Trehalose and glycogen...”
- Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function
Chen, Journal of bacteriology 2007 - “...pv. syringae B728a, PA3891 in P. aeruginosa PAO1, PP0868 in P. putida KT2440, PSPPH4276 in P. syringae pv. phaseolicola 1448A, PFL0868 in P. fluorescens...”
- “...monocytogenes OpuCA (4, 54); PSPTO_4575, Psyr4249, PA3891, and PP0868 (this study); YehX (lack of uptake in MKH13, which contains an intact yehX); Lmo1421 (3,...”
TC 3.A.1.12.8 / Q93A35 BilEA aka OpuBA protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) from Listeria monocytogenes (see 2 papers)
NP_464946 glycine/betaine ABC transporter ATP-binding protein from Listeria monocytogenes EGD-e
lmo1421 similar to glycine betaine/carnitine/choline ABC transporter (ATP-binding protein) from Listeria monocytogenes EGD-e
36% identity, 88% coverage
- substrates: Glycine betaine, Proline
- Enhancing bile tolerance improves survival and persistence of Bifidobacterium and Lactococcus in the murine gastrointestinal tract
Watson, BMC microbiology 2008 - “...(accession number NC_003210) uncovered the presence of two Open Reading Frames (ORFs) bilEA (accession number NP_464946 and GI number GI:16803461) and bilEB (accession number is NP_464947.1 and GI number is GI:16803462), oriented in the same direction and overlapping by five nucleotides. PCR primers with incorporated Xba...”
- Effects of intrinsic and extrinsic growth factors on virulence gene expression of foodborne pathogens in vitro and in food model systems; a review
Hosseini, Food science & nutrition 2024 - “...ham under HPP. The regulation of prfA , plcA , hly , sigB , and lmo1421 virulence genes was severely downregulated in one strain compared in another strain (PrezBaltar etal., 2021 ). L . monocytogenes can become more adaptable to HPP and other stressful conditions due...”
- Impact of High-Pressure Processing (HPP) on Listeria monocytogenes-An Overview of Challenges and Responses
Wiśniewski, Foods (Basel, Switzerland) 2023 - “...Boor K.J. Wiedmann M. SigmaB -dependent expression patterns of compatible solute transporter genes opuCA and lmo1421 and the conjugated bile salt hydrolase gene bsh in Listeria monocytogenes Microbiology 2003 149 3247 3256 10.1099/mic.0.26526-0 14600237 137. Bowman J.P. Bittencourt C.R. Ross T. Differential gene expression of Listeria...”
- “...5 2 2 strains 450 10 16 prfA , plcA , hly , sigB , lmo1421 [ 128 ] 600 5 3 1 strain 200 3 12 luxS Upregulation Trypticase soy broth (TSB) [ 15 ] 400 4 2 strains 200 8 8 sigB , hpf...”
- Human Listeriosis
Koopmans, Clinical microbiology reviews 2023 (secret) - Effect of high pressure processing on the inactivation and the relative gene transcription patterns of Listeria monocytogenes in dry-cured ham
Pérez-Baltar, Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire 2021 - Characterization of the biofilm phenotype of a Listeria monocytogenes mutant deficient in agr peptide sensing
Zetzmann, MicrobiologyOpen 2019 - “...be involved in resistance to cell wall and osmotic stress (Supplementary File S1). For example, lmo1421 , lmo1425 , lmo1426 , and lmo1427 encode for components of the ABC transporters OpuA and OpuC, which are required for transport of the compatible solutes glycine/betaine, carnitine, and ornithine...”
- Food-Associated Stress Primes Foodborne Pathogens for the Gastrointestinal Phase of Infection
Horn, Frontiers in microbiology 2018 - “...) and in salami, acidic ( B and lmo0669 ) and osmotic ( gbuA and lmo1421 ) stress-related genes were upregulated ( Mataragas et al., 2015 ). Table 2 Effect of food products on virulence potential of foodborne pathogens. Pathogen Food Virulence potential Reference Bacillus spp....”
- “...et al., 2010 Salami Acidic ( B and lmo0669 ) and osmotic ( gbuA and lmo1421 ) stress-related genes were upregulated Mataragas et al., 2015 Pear and Melon Increased adhesion and invasion of Caco-2 cells after 2 days of storage Cols-Med et al., 2017 Milk Increased...”
- Cyclic di-AMP targets the cystathionine beta-synthase domain of the osmolyte transporter OpuC
Huynh, Molecular microbiology 2016 - “...the carnitine transporter component OpuCA. (A) Domain organization of OpuCA (Lmo1428), GbuA (Lmo1014), and BilE (Lmo1421). (B) Binding of OpuCA, GbuA, and BilE to c-di-AMP as measured by DRaCALA to full-length (FL), ATP-binding (ATP), or CBS domains. (C) Binding titration for the OpuCA full-length OpuCA protein...”
- Differential gene expression profiling of Listeria monocytogenes in Cacciatore and Felino salami to reveal potential stress resistance biomarkers
Mataragas, Food microbiology 2015 (PubMed)- “...and ripening of Cacciatore, whereas gbuA and lmo1421 were up-regulated during the ripening of Felino and Cacciatore, respectively. sigB expression was...”
- “...gene expression profiling analysis suggested that sigB and lmo1421 or sigB and gbuA could be used as different types of stress resistance biomarkers to...”
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PA0302 polyamine transport protein PotG from Pseudomonas aeruginosa PAO1
Q9I6I9 Spermidine/putrescine import ATP-binding protein PotA from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
37% identity, 75% coverage
- Putrescine and Its Metabolic Precursor Arginine Promote Biofilm and c-di-GMP Synthesis in Pseudomonas aeruginosa
Liu, Journal of bacteriology 2022 (secret) - Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity
Krysenko, Medical sciences (Basel, Switzerland) 2022 - “...substrate-binding protein PotF (b0854) SpuE (PA0301) SCO5667 Polyamine ABC transporter substrate-binding protein PotG (b0855) SpuF (PA0302) SCO5668 Polyamine ABC-transporter integral membrane protein PotH (b0856) SpuG (PA0303) SCO5669 Polyamine ABC-transporter integral membrane protein PotI (b0857) SpuH (PA0304) SCO5670 -glutamyl-polyamine oxidoreductase PuuB (b1301) PauB3 (PA2776) SCO5671 -aminobutyrate aminotransferase...”
- Sequence diversity of the Pseudomonas aeruginosa population in loci that undergo microevolution in cystic fibrosis airways
Fischer, Access microbiology 2021 - “...spoT PA5338 2106 Guanosine-3,5-bis(diphosphate) 3-pyrophosphohydrolase spuE PA0301 1098 Polyamine transport protein 8 (spermidine-binding protein) spuF PA0302 1155 Polyamine transport protein PotG (ABC transporter ATPase) Strain panel and sequencing The strain panel consisted of 345 airway isolates from patients with CF, 25 airway isolates from patients with...”
- Differential transcription profiling of the phage LUZ19 infection process in different growth media
Brandão, RNA biology 2021 (secret) - Pseudomonas aeruginosa Presents Multiple Vital Changes in Its Proteome in the Presence of 3-Hydroxyphenylacetic Acid, a Promising Antimicrobial Agent
Ozdemir, ACS omega 2020 - “...response to hypochlorite redox and cell homeostasis Q9I6I9 spermidine/putrescine import ATP-binding protein PotA spuF potA, PA0302 spermidine transport, putrescine transport redox and cell homeostasis Q9HUX1 biosynthetic arginine decarboxylase speA PA4839 arginine catabolic process, putrescine biosynthetic process, spermidine biosynthetic process redox and cell homeostasis Q9X6R0 polyamine aminopropyltransferase1...”
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - Pseudomonas aeruginosa Presents Multiple Vital Changes in Its Proteome in the Presence of 3-Hydroxyphenylacetic Acid, a Promising Antimicrobial Agent
Ozdemir, ACS omega 2020 - “...reductase MsrA msrA PA5018 response to oxidative stress, response to hypochlorite redox and cell homeostasis Q9I6I9 spermidine/putrescine import ATP-binding protein PotA spuF potA, PA0302 spermidine transport, putrescine transport redox and cell homeostasis Q9HUX1 biosynthetic arginine decarboxylase speA PA4839 arginine catabolic process, putrescine biosynthetic process, spermidine biosynthetic...”
AMK58_16560 ABC transporter ATP-binding protein from Azospirillum brasilense
47% identity, 57% coverage
W6QXK5 Spermidine/putrescine import ATP-binding protein PotA from Ectopseudomonas oleovorans (strain CECT 5344)
43% identity, 60% coverage
HVX45_RS02390 osmoprotectant ABC transporter ATP-binding protein OsmV from Escherichia fergusonii
31% identity, 93% coverage
- OrthoRefine: automated enhancement of prior ortholog identification via synteny
Ludwig, BMC bioinformatics 2024 - “.... coli genes (b0652, b3271, b4106, & b4096), fives genes from E . fergusonii (HVX45_RS09410, HVX45_RS02390, HVX45_RS04025, HVX45_RS07420, & HVX45_RS11505), two genes from E . marmotae (GV529_RS14465 & GV529_RS05870), and one gene from E . albertii (JRC41_RS15115). Most of the genes were annotated as encoding ATP-binding...”
- “...& GV529_RS05870 and SOG19.1 which consisted of b3271, HVX45_RS11505, & GV529_RS14465, while excluding b4106, b4096, HVX45_RS02390, HVX45_RS04205, & HVX45_RS09410 as presumed paralogs (Fig. 4 ). The BLAST e-values and percent identity from OrthoFinders alignment supported the presence of these two natural subgroups in HOG19 (Additional file...”
ESA_01738 osmoprotectant ABC transporter ATP-binding protein OsmV from Cronobacter sakazakii ATCC BAA-894
32% identity, 93% coverage
- Characterization of the Desiccation Tolerance of Cronobacter sakazakii Strains
Du, Frontiers in microbiology 2018 - “...protein F: GACGCCGATTTTCCTTGAGAT R: GGCGACATCAGGATCACAAC proP (ESA_02131) Proline/glycine betaine transporter F: CGCCTGTCCTGCTGTTACTCTG R: CGAAGCCCGCAATAGAGCC opuCA (ESA_01738) glycine/betaine ABC transporter ATP-binding protein OpuCA F: GGACAAACAGCCGACGATTACA R: GCTTCTCCCGGAACGCATAT opuCC (ESA_01740) glycine/betaine ABC transporter substrate-binding protein OpuCC F: GCGGCTGTTATGACGATGCT R: GACGGCTCATGCCTTTTTTC otsA (ESA_01335) Betaine aldehyde dehydrogenase F: CGGTGAAATCGGCAATGAG R:...”
- “...2 ). After drying treatment, proline/glycine betaine transporter (ESA_02131), glycine/betaine ABC transporter ATP-binding protein OpuCA (ESA_01738), glycine/betaine ABC transporter substrate-binding protein OpuCC (ESA_01740), trehalose-6-phosphate synthase OtsA (ESA_01335), and choline dehydrogenase BetA (ESA_02049) showed a significantly upregulated expression ( p < 0.05). Compared with strain ENS 70819...”
FORC67_1458 betaine/proline/choline family ABC transporter ATP-binding protein from Listeria monocytogenes
33% identity, 87% coverage
- Multi-Target Antibacterial Mechanism of Moringin From Moringa oleifera Seeds Against Listeria monocytogenes
Wen, Frontiers in microbiology 2022 - “...FORC67_1632 FORC67_1632-F GGTACTGGAAGCGGGACAAA FORC67_1632-R TGGATGGTGCGCGTAATCAT FORC67_1456 FORC67_1456-F CAGGACTTGGAGGCAGTTCC FORC67_1456-R TAACCAAGCGCCACATCCAT FORC67_1457 FORC67_1457-F CGGCCATAACTTGCTTGTTCA FORC67_1457-R AACAGGAATTGCCCCACCAA FORC67_1458 FORC67_1458-F TAAACTGGCGGACCGTATCG FORC67_1458-R CGCATCTTCTACCGTACCCC FORC67_0722 FORC67_0722-F ACGATGCAATGTTCATGCGT FORC67_0722-R CTCTAAAACCCGGTCCGCTT FORC67_0533 FORC67_0533-F AAGTGCGTGCGGTACTAGAC FORC67_0533-R TCGCGAGTATTTCATCGATTGT The Effect of Moringin on the Cell Wall and Cell Membrane of Listeria monocytogenes Scanning Electron...”
lin1467 similar to glycine betaine/carnitine/choline ABC transporter (ATP-binding protein) from Listeria innocua Clip11262
33% identity, 87% coverage
- Comparative analysis of the sigma B-dependent stress responses in Listeria monocytogenes and Listeria innocua strains exposed to selected stress conditions
Raengpradub, Applied and environmental microbiology 2008 - “...for L. innocua lin0285 (rpoB), lin0289, lin0942 (fri), lin1467 (opuCA), lin1992 (cspD), lin2528 (gadA), lin2553 (gap), and lin2891 and for L. monocytogenes...”
- “...change the conclusions (Fig. 1 legend). Finally, lmo1428/ lin1467 (encoding OpuCA, a component of the carnitine transporter) were included in the qRT-PCR...”
LSA0616 Putative glycine/betaine/carnitine/choline ABC transporter, ATP-binding subunit from Lactobacillus sakei subsp. sakei 23K
34% identity, 88% coverage
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...-0.6 LSA0513 lsa0513 Putative stress-responsive transcriptional regulator -0.8 LSA0552 lsa0552 Organic hydroperoxide resistance protein 0.6 LSA0616 lsa0616 Putative glycine/betaine/carnitine/choline ABC transporter, ATP-binding subunit 0.9 LSA0617 lsa0617 Putative glycine/betaine/carnitine/choline ABC transporter, membrane-spanning subunit 1.3 LSA0618 lsa0618 Putative glycine/betaine/carnitine/choline ABC transporter, substrate-binding lipoprotein 0.6 LSA0619 lsa0619 Putative glycine/betaine/carnitine/choline...”
- Analysis of Lactobacillus sakei mutants selected after adaptation to the gastrointestinal tracts of axenic mice
Chiaramonte, Applied and environmental microbiology 2010 - “...to oxidative stresses. Five proteins (LSA0705, LSA0706, LSA0938, LSA0616, and LSA1366) are components of ABC TABLE 2. Growth characteristics of L. sakei 23K, L....”
- “...2.1 3.5 LSA0706 0.007 3.4 LSA0938 0.026 3.3 LSA0616 0.023 3.1 LSA0104 LSA0030 LSA1712 LSA1779 LSA1134 LSA1426 LSA0494 LSA1544 0.003 0.001 0.001 0.023 0.037...”
OPUCA_LISM4 / G2JZ44 Carnitine transport ATP-binding protein OpuCA; EC 7.6.2.9 from Listeria monocytogenes serotype 1/2a (strain 10403S) (see 4 papers)
OPUCA_LISMN / Q9KHT9 Carnitine transport ATP-binding protein OpuCA; EC 7.6.2.9 from Listeria monocytogenes (see paper)
LMON_1491 betaine/proline/choline family ABC transporter ATP-binding protein from Listeria monocytogenes EGD
Q7AP65 Quaternary amine transport ATP-binding protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
lmo1428 similar to glycine betaine/carnitine/choline ABC transporter (ATP-binding protein) from Listeria monocytogenes EGD-e
33% identity, 87% coverage
- function: Part of the ABC transporter complex OpuCABCD involved in carnitine uptake. Probably responsible for energy coupling to the transport system. Involved, with BetL and GbuABC, in osmoprotection and cryoprotection of Listeria. Can also mediate weak glycine betaine transport.
catalytic activity: a quaternary ammonium(out) + ATP + H2O = a quaternary ammonium(in) + ADP + phosphate + H(+) (RHEA:11036)
subunit: The complex is composed of two ATP-binding proteins (OpuCA), two transmembrane proteins (OpuCB and OpuCD) and a solute-binding protein (OpuCC). - function: Part of the ABC transporter complex OpuCABCD involved in carnitine uptake. Probably responsible for energy coupling to the transport system. Involved, with BetL and GbuABC, in osmoprotection and cryoprotection of Listeria.
catalytic activity: a quaternary ammonium(out) + ATP + H2O = a quaternary ammonium(in) + ADP + phosphate + H(+) (RHEA:11036)
subunit: The complex is composed of two ATP-binding proteins (OpuCA), two transmembrane proteins (OpuCB and OpuCD) and a solute-binding protein (OpuCC).
disruption phenotype: Mutants are impaired for growth at high osmolarity in brain heart infusion broth, fail to grow in a defined medium and show no detectable carnitine uptake. - Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Hingston, PloS one 2017 - “...activated L-carnitine/choline ABC transporter, permease protein OpuCB opuCB lmo1427 10 2.51 5.28 3.78 1.58 1.10 LMON_1491 Osmotically activated L-carnitine/choline ABC transporter, ATP-binding protein OpuCA opuCA lmo1428 10 2.63 5.54 3.94 1.30 -0.01 LMON_1932 Predicted membrane protein hemolysin III + lmo1864 14 2.21 1.58 1.78 1.84 1.06...”
- “...1.24 LMON_1490 L-carnitine/choline ABC transporter, permease protein opuCB lmo1427 10 2.51 5.28 3.78 1.58 1.10 LMON_1491 L-carnitine/choline ABC transporter, ATP-binding protein opuCA lmo1428 10 2.63 5.54 3.94 1.30 -0.01 LMON_1761 Hypothetical protein + lmo1694 10 3.78 5.06 2.55 -0.95 0.67 LMON_2234 Hypothetical protein lmo2158 12 3.80...”
- A decade of research on the second messenger c-di-AMP
Yin, FEMS microbiology reviews 2020 - “...M 1.2 M NAa NA AAW38543 CAC99506 CAB15388 CAD45880 A0A160MQL0 Q7AP65 O34992 Q8E7C0 Schuster et al. 2016 Huynh et al. 2016 Gundlach et al. 2019 Devaux et al....”
- Listeria monocytogenes Biofilm Adaptation to Different Temperatures Seen Through Shotgun Proteomics
Santos, Frontiers in nutrition 2019 - “...the carnitine transporter OpuC ( 25 ). Here, we have identified GbuB (Q7AP75) and OpuCA (Q7AP65). The non-identification of a BetL can be reasoned with the described more pronounced role of Gbu and OpuC as preferential cryoprotection systems in L. monocytogenes ( 49 ). Regarding the...”
- “...than betaine after cold temperature ( 48 ). Supporting this result, GbuB (Q7AP75) and OpuCA (Q7AP65) were in this study more abundant at 10C. Interestingly, OpuCA had one of the highest fold changes (FC = 4.91, Figure 8A ; FC = 5.56, Figure 8B ), one...”
- Ribosomal mutations enable a switch between high fitness and high stress resistance in Listeria monocytogenes
Koomen, Frontiers in microbiology 2024 - “...et al., 2010 ) and subunits of the known OpuC glycine betaine osmolyte transporter OpuCA (lmo1428) and OpuCC (lmo1426). SigB (lmo0895) itself was upregulated but did not pass the stringent cut-off values applied to the proteomics data (>1 or<1 log 10 (protein ratio), with adjusted -log...”
- The population structure and genetic diversity of Listeria monocytogenes ST9 strains based on genomic analysis
Song, Frontiers in microbiology 2022 - “...lmo0913, lmo0036 , and lmo1367 ), high salt concentration ( lmo2748, lmo2092, lmo1014, lmo1015, lmo1016, lmo1428, lmo1427, lmo1426 , and lmo1425 ), and biofilm formation ( lmo0673, lmo0435, lmo1460, lmo2504, lmo1288 , and inlL ), and SSI-1 containing five genes ( lmo0444lmo0448 ) to resist acidic,...”
- “...lmo1722, lmo1378 , and lmo0288 ), high salt concentration ( lmo2748, lmo2092, lmo1014, lmo1015, lmo1016, lmo1428, lmo1427, lmo1426 , and lmo1425 ), and SSI-1 ( lmo0444, lmo0445, lmo0446, lmo0447 , and lmo0448 ) are shown. Click here for additional data file. Supplementary Figure S4 PMSCs in...”
- Different Shades of Listeria monocytogenes: Strain, Serotype, and Lineage-Based Variability in Virulence and Stress Tolerance Profiles
Muchaamba, Frontiers in microbiology 2021 - “...BetL lmo2092 Glycine betaine transporter 10 Gbu lmo1014 lmo1016 Glycine betaine transporter 5 OpuC lmo1425 lmo1428 Carnitine transporter 4 KdpED lmo2678 lmo2679 Kdp ATPase, two-component system for K+ uptake 36 KdpABC lmo2680 lmo2682 Potassium-transporting ATPase 21 ProBA lmo1259 lmo1260 Proline biosynthesis 31 OppA lmo2196 Oligopeptide transporter...”
- Listeria monocytogenes σA Is Sufficient to Survive Gallbladder Bile Exposure
Boonmee, Frontiers in microbiology 2019 - “...transporter, permease protein OpuCD, subunit of predicted ATP-driven transporter complex of CARNITINE/choline 2.29 0.00 LMRG_00880 lmo1428 opuCA Osmotically activated L-carnitine/choline ABC transporter, ATP-binding protein OpuCA, subunit of predicted ATP-driven transporter complex of CARNITINE/choline 2.05 0.00 LMRG_00926 lmo1473 dnaK hrcA-grpE-dnaK-dnaJ Chaperone protein, DnaK 2.39 0.00 LMRG_01023 lmo1877...”
- Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Hingston, PloS one 2017 - “...2.51 5.28 3.78 1.58 1.10 LMON_1491 Osmotically activated L-carnitine/choline ABC transporter, ATP-binding protein OpuCA opuCA lmo1428 10 2.63 5.54 3.94 1.30 -0.01 LMON_1932 Predicted membrane protein hemolysin III + lmo1864 14 2.21 1.58 1.78 1.84 1.06 LMON_2054 Dihydroxy-acid dehydratase ilvD + lmo1983 4 * 3.52 3.89...”
- “...opuCB lmo1427 10 2.51 5.28 3.78 1.58 1.10 LMON_1491 L-carnitine/choline ABC transporter, ATP-binding protein opuCA lmo1428 10 2.63 5.54 3.94 1.30 -0.01 LMON_1761 Hypothetical protein + lmo1694 10 3.78 5.06 2.55 -0.95 0.67 LMON_2234 Hypothetical protein lmo2158 12 3.80 5.25 3.18 0.77 3.03 LMON_2306 Arsenate reductase...”
- Cyclic di-AMP targets the cystathionine beta-synthase domain of the osmolyte transporter OpuC
Huynh, Molecular microbiology 2016 - “...was assessed for bacterial growth density. Protein expression and purification for crystalization L. monocytogenes OpuCA (lmo1428) residues 247371 were subcloned into a pET28a vector with an N-terminal His-tag. This construct was then transformed into BL21 Star (DE3) cells. The cells were cultured in LB (LuriaBertani) medium...”
- “...with the CBS domain of the carnitine transporter component OpuCA. (A) Domain organization of OpuCA (Lmo1428), GbuA (Lmo1014), and BilE (Lmo1421). (B) Binding of OpuCA, GbuA, and BilE to c-di-AMP as measured by DRaCALA to full-length (FL), ATP-binding (ATP), or CBS domains. (C) Binding titration for...”
- Protein level identification of the Listeria monocytogenes sigma H, sigma L, and sigma C regulons
Mujahid, BMC microbiology 2013 - “...positively regulated by any of the alternative factors include Lmo1540, Lmo2610, Lmo1422, Lmo1421, Lmo1602, Lmo1426, Lmo1428, Lmo2205, Lmo2398, Lmo1601, Lmo0554, Lmo1634, Lmo0110, Lmo2558, Lmo0783, Lmo0134, and Lmo0098. Table 4 Proteins found to be differentially regulated by at least two of the three alternative sigma factors studied...”
- Listeria monocytogenes shows temperature-dependent and -independent responses to salt stress, including responses that induce cross-protection against other stresses
Bergholz, Applied and environmental microbiology 2012 - “...LMOh7858_3076 lmo0201 lmo0211 lmo0433 lmo0955 lmo1014 lmo1022 lmo1428 lmo1473 lmo1580 lmo2067 lmo2069 lmo2092 lmo2196 lmo2748 lmo2754 lmo2785 lmo2812 37C 7C...”
- More
LMRG_00880 glycine betaine/L-proline ABC transporter, ATP-binding protein from Listeria monocytogenes 10403S
33% identity, 85% coverage
- Listeria monocytogenes σA Is Sufficient to Survive Gallbladder Bile Exposure
Boonmee, Frontiers in microbiology 2019 - “...ABC transporter, permease protein OpuCD, subunit of predicted ATP-driven transporter complex of CARNITINE/choline 2.29 0.00 LMRG_00880 lmo1428 opuCA Osmotically activated L-carnitine/choline ABC transporter, ATP-binding protein OpuCA, subunit of predicted ATP-driven transporter complex of CARNITINE/choline 2.05 0.00 LMRG_00926 lmo1473 dnaK hrcA-grpE-dnaK-dnaJ Chaperone protein, DnaK 2.39 0.00 LMRG_01023...”
RHE_RS17470 ABC transporter ATP-binding protein from Rhizobium etli CFN 42
39% identity, 64% coverage
Sca_0455 ABC transporter ATP-binding protein from Staphylococcus carnosus subsp. carnosus TM300
SCA_0455 methionine ABC transporter ATP-binding protein from Staphylococcus carnosus subsp. carnosus TM300
44% identity, 56% coverage
PH0194 multiple sugar-binding transport ATP-binding protein from Pyrococcus horikoshii OT3
43% identity, 58% coverage
2d62A / O57933 Crystal structure of multiple sugar binding transport atp-binding protein
43% identity, 58% coverage
- Ligand: pyrophosphate 2- (2d62A)
lp_0368 ABC transporter ATP-binding protein from Lactiplantibacillus plantarum WCFS1
lp_0368 glycine betaine/carnitine/choline ABC transporter, ATP-binding protein from Lactobacillus plantarum WCFS1
42% identity, 60% coverage
SXYL_00488 betaine/proline/choline family ABC transporter ATP-binding protein from Staphylococcus xylosus
35% identity, 87% coverage
MSMEG_2926 glycine betaine/carnitine/choline transport ATP-binding protein opuCA from Mycobacterium smegmatis str. MC2 155
36% identity, 93% coverage
- Characterization of Mycobacterium smegmatis sigF mutant and its regulon: overexpression of SigF antagonist (MSMEG_1803) in M. smegmatis mimics sigF mutant phenotype, loss of pigmentation, and sensitivity to oxidative stress
Singh, MicrobiologyOpen 2015 - “...Permease bindingprotein component 5.78/3.65 GTTTN 16 GGGTA MSMEG_2925 Permease membrane component 5.77/5.79 GTTTN 16 GGGTA MSMEG_2926 Glycine betaine/carnitine/choline transport 5.77/4.41 GTTTN 16 GGGTA MSMEG_2927 a ABC transporter, permease protein OpuCB 4.94/4.06 GTTTN 16 GGGTA 39 MSMEG_2958 a Conserved hypothetical protein 3.79/5.19 GTTCN 15 GGGTA 24 MSMEG_3022...”
- Gene Expression, Bacteria Viability and Survivability Following Spray Drying of Mycobacterium smegmatis
Lauten, Materials (Basel, Switzerland) 2010 - “...expressed were genes involved in the acquisition or production of osmolytes and carotenoid antioxidants (e.g. MSMEG_2926 and MSMEG_3184; MSMEG_2345 and MSMEG_2346), two catalases (MSMEG_6213; MSMEG_6232), and the starvation-induced DNA protecting protein (MSMEG_6467). 2.3.4. Stationary Phase Comparison Stationary phase microarray data did not have any statistically significant...”
- “...permease binding-protein component 0.7 10.2 0.021 90% MSMEG_2925 permease membrane component 0.9 8.9 0.008 92% MSMEG_2926 glycine betaine/carnitine/choline transport ATP-binding protein opuCA 1.0 10.1 0.006 98% MSMEG_2958 conserved hypothetical protein 1.0 10.3 0.003 98% MSMEG_3022 transglycosylase associated protein 1.6 10.5 0.008 94% MSMEG_3184 malto-oligosyltrehalose trehalohydrolase treZ...”
- The SigF regulon in Mycobacterium smegmatis reveals roles in adaptation to stationary phase, heat, and oxidative stress
Hümpel, Journal of bacteriology 2010 - “...MSMEG_2346 MSMEG_2347 MSMEG_2376 MSMEG_2913 MSMEG_2925 MSMEG_2926 MSMEG_3184 MSMEG_3186 MSMEG_3304 MSMEG_3311 MSMEG_3418 MSMEG_3536 MSMEG_3541 MSMEG_3543...”
OSMV_SALTY / Q8ZPK4 Osmoprotectant import ATP-binding protein OsmV; EC 7.6.2.- from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
TC 3.A.1.12.14 / Q8ZPK4 ABC-type proline/glycine betaine transport system, component of OsmU (OsmVWXY) transporter for glycine betaine and choline-O-sulfate uptake. Induced by osmotic stress (0.3M NaCl) (Frossard et al., 2012). Also called OpuCA/CB1/CB2/CC from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
STM1491 ABC-type proline/glycine betaine transport systems, ATPase component from Salmonella typhimurium LT2
SL1344_RS07410 osmoprotectant ABC transporter ATP-binding protein OsmV from Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
32% identity, 93% coverage
- function: Part of the OsmU ABC transporter complex, which is involved in the uptake of osmoprotectants such as choline-O-sulfate and glycine betaine. Probably responsible for energy coupling to the transport system.
subunit: The complex is composed of two ATP-binding proteins (OsmV), two transmembrane proteins (OsmW and OsmY) and a solute-binding protein (OsmX).
disruption phenotype: Deletion of the osmU operon eliminates the residual osmoprotection by glycine betaine in a mutant that is lacking the ProP and the ProU systems. OsmU deletion has no effect on the utilization of glycine betaine as an osmoprotectant when ProP or ProU are functional. - substrates: Choline-O-sulfate, Glycine betaine
- General response of Salmonella enterica serovar Typhimurium to desiccation: A new role for the virulence factors sopD and sseD in survival
Maserati, PloS one 2017 - “...methyltransferase A 2.05 Transporters STM0006 yaaJ alanine/glycine transport protein 5.48 STM3986 trkH potassium transporter 3.70 STM1491 osmV proline/glycine betaine transport systems 2.91 STM1379 orf48 amino acid permease 2.51 STM2353 hisQ histidine/lysine/arginine/ornithine transport protein 2.37 STM1893 znuB high-affinity zinc transporter membrane protein 2.28 STM1806 nhaB sodium/proton antiporter...”
- Exposure of Salmonella enterica Serovar Typhimurium to Three Humectants Used in the Food Industry Induces Different Osmoadaptation Systems
Finn, Applied and environmental microbiology 2015 - “...upregulation of the osmoprotective transporter mechanisms encoded by the proP , proU , and osmU (STM1491 to STM1494) genes. Glycerol exposure elicited the downregulation of these osmoadaptive mechanisms but stimulated an increase in lipopolysaccharide and membrane protein-associated genes after 1 h. The most extensive changes in...”
- “...were downregulated following exposure to glycerol. These included the proP , proU , and osmU (STM1491 to STM1494) osmoprotectant transporter genes as well as genes for glutamate formation and trehalose biosynthesis (both via otsAB and conversion via glycogen). In contrast, at this time point the putP...”
- ProP is required for the survival of desiccated Salmonella enterica serovar typhimurium cells on a stainless steel surface
Finn, Applied and environmental microbiology 2013 - “...culture. The osmoprotectant transporters proP, proU, and osmU (STM1491 to STM1494) were highly upregulated by drying. Deletion of any one of these transport...”
- “...proVWX operon. The OsmU ABC transporter system (the STM1491 to STM1494 genes, known as osmVWXY) was also upregulated under desiccation stress (36). This system...”
- A glycine betaine importer limits Salmonella stress resistance and tissue colonization by reducing trehalose production
Pilonieta, Molecular microbiology 2012 - “...). A previous study used DNA microarrays to identify genes potentially regulated by RcsBCD, including STM1491 and STM1492 ( Erickson and Detweiler, 2006 ). STM1491 and STM1492 are part of a four-gene putative operon that has significant homology to Osmoprotectant uptake locus C (OpuC), within Gram-negative...”
- “...under high osmolarity ( Chen and Beattie, 2007 ). In addition, the OpuA ATPases, including STM1491, are characterized by tandem cystathionine--synthase (CBS) domains, which are required for and predictive of functional osmoregulatory transport ( Figure 1B ) ( Chen and Beattie, 2007 ; Biemans-Oldehinkel et al.,...”
- Identification of a third osmoprotectant transport system, the osmU system, in Salmonella enterica
Frossard, Journal of bacteriology 2012 - “...osmU deletion. The four genes of the osmU operon, STM1491 to STM1494, are located on the minus strand of the S. Typhimurium LT2 sequence (GenBank accession no....”
- “...from the 3= end of the last gene of the operon (STM1491). The 3= end of the clcB (STM1495) gene, which is the next gene upstream of the osmU operon, is at...”
- Isolation and preliminary characterization of amino acid substitution mutations that increase the activity of the osmoregulated ProP protein of Salmonella enterica serovar Typhimurium
Gasper, DNA and cell biology 2012 - “...high osmolarity (Gutierrez and Csonka, 1989), encoded by the STM1491 to STM1494 genes. This transporter, which does not take up proline, is absent from E. coli...”
- Rcs and PhoPQ regulatory overlap in the control of Salmonella enterica virulence
García-Calderón, Journal of bacteriology 2007 - “...r171 r150 r153 r172 melB yhhJ srfA PSLT071 yiaD STM1491 1.9 3.3 9.5 4.5 17.7 7.4 trg srfABC bapABCD yjbEFGH narGHJI siiABCDEF dcuBfumB STM2179- STM2178-...”
- “...STM2175 melAB yhiHyhhJ srfABC PSLT071 yiaD STM1494- STM1493- STM1492- STM1491 Insertion(s)a 53, 81 93 51 29 8, 79, 80 63, 92 29, 38 58 62, 66, 70,...”
- The Rcs phosphorelay system is specific to enteric pathogens/commensals and activates ydeI, a gene important for persistent Salmonella infection of mice
Erickson, Molecular microbiology 2006 - “...STM4239b STM4222 STM2983 STM4336b STM4240 STM3269 STM1285b STM1491 STM3443 STM1515 STM1492 STM3363 STM2311 STM2795 STM1589 STM1284 STM4451 STM0615 STM1952...”
- “...distinct putative ABC transporter operons were identified (STM1491, STM1492 and STM1951), one of which (STM1491-1494) is Salmonella spp.-specific. Four of the...”
- More
- Effects of colonization-associated gene yqiC on global transcriptome, cellular respiration, and oxidative stress in Salmonella Typhimurium
Fan, Journal of biomedical science 2022 - “...fdx , nifU , and yhgI ), osmoprotectant ABC transporter substrate-binding proteins (SL1344_RS07420, SL1344_RS07415, and SL1344_RS07410), NADH dehydrogenase ( ndh ), and some hypothetical proteins. Table 1 RNA-seq analysis revealed the 20 most significantly upregulated genes ( A ) and the 20 most significantly downregulated genes...”
A0A142UTH4 ABC-type polyamine transporter (subunit 4/4) (EC 7.6.2.11) from Streptococcus suis (see paper)
SSU0751 spermidine/putrescine ABC transporter ATP-binding protein from Streptococcus suis P1/7
42% identity, 58% coverage
SSP1997 putative ABC-type proline glycine betaine transport system ATPase component from Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305
42% identity, 58% coverage
SXYL_02128 ABC transporter ATP-binding protein from Staphylococcus xylosus
42% identity, 58% coverage
C289_0889 ABC transporter ATP-binding protein from Anoxybacillus ayderensis
43% identity, 54% coverage
- Analysis of anoxybacillus genomes from the aspects of lifestyle adaptations, prophage diversity, and carbohydrate metabolism
Goh, PloS one 2014 - “...revealed the presence of genes coding for spermidine synthases (C289_0840, F510_0514) and spermidine transport proteins (C289_0889, C289_0890, C289_0891, C289_0892; F510_563, F510_564, F510_565, F510_566). The enzymes were mapped to Kyoto Encyclopedia of Genes and Genomes, KEGG pathways (map 330), which indicated that spermidine and spermine contributed to...”
SMc00771 PROBABLE PUTRESCINE TRANSPORT ATP-BINDING PROTEIN from Sinorhizobium meliloti 1021
39% identity, 70% coverage
BC1324 2-aminoethylphosphonate transport ATP-binding protein phnT from Bacillus cereus ATCC 14579
36% identity, 72% coverage
SSUSC84_1444 ABC-type glycine betaine transport system ATP-binding protein from Streptococcus suis SC84
43% identity, 56% coverage
U876_00745 polyamine ABC transporter ATP-binding protein from Aeromonas hydrophila NJ-35
42% identity, 60% coverage
WP_099424991 methionine ABC transporter ATP-binding protein from Solibacillus sp. R5-41
43% identity, 57% coverage
E2P69_RS06565 ABC transporter ATP-binding protein from Xanthomonas perforans
42% identity, 66% coverage
BPSL1739 putative ABC transport system, ATP-binding protein from Burkholderia pseudomallei K96243
45% identity, 56% coverage
K3G22_05085 polyamine ABC transporter ATP-binding protein from Shewanella putrefaciens
44% identity, 57% coverage
RSAU_000696 ABC transporter ATP-binding protein from Staphylococcus aureus subsp. aureus 6850
41% identity, 58% coverage
SAUSA300_0706 putative osmoprotectant ABC transporter, ATP-binding protein from Staphylococcus aureus subsp. aureus USA300_FPR3757
NWMN_0690 osmoprotectant ABC transporter, ATP-binding protein from Staphylococcus aureus subsp. aureus str. Newman
41% identity, 58% coverage
- Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Genetic Factors Involved in Human Endothelial Cells Damage, an Important Phenotype Correlated with Persistent Endovascular Infection
Xiao, Antibiotics (Basel, Switzerland) 2022 - “...25.07 9.15 SAUSA300_1266 trpF N-(5-phosphoribosyl)anthranilate isomerase 25.05 7.12 SAUSA300_2251 hypothetical dehydrogenase family protein 25.00 3.65 SAUSA300_0706 hypothetical putative osmoprotectant ABC transporter ATP-binding protein 24.95 11.00 SAUSA300_0941 hypothetical putative ferrichrome ABC transporter 24.69 6.43 SAUSA300_0951 sspA V8 protease 24.55 8.41 SAUSA300_1875 hypothetical exonuclease 24.52 10.68 SAUSA300_0566 hypothetical...”
- Bacterial Membrane-Derived Vesicles Attenuate Vancomycin Activity against Methicillin-Resistant Staphylococcus aureus
Kumaraswamy, Microorganisms 2021 - “...protein SAUSA300_1690 Putative thioredoxin HMPREF0776_2533 Alpha-amylase SAUSA300_0289 Uncharacterized protein lysS LysinetRNA ligase pyrG CTP synthase SAUSA300_0706 Putative osmoprotectant ABC transporter, ATP-binding protein SA0224 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain protein recA Protein RecA HMPREF0776_0345 Accessory regulator family SAUSA300_2132 UPF0457 protein SAUSA300_2132 accC Acetyl-CoA carboxylase, biotin carboxylase HMPREF0776_2830...”
- Comparative Transcriptomics Reveals Discrete Survival Responses of S. aureus and S. epidermidis to Sapienic Acid
Moran, Frontiers in microbiology 2017 - “...transporters were upregulated in response to sapienic challenge in S. aureus (NWMN_2457, NWMN_2050, NWMN_2089 and NWMN_0690) and S. epidermidis (SETU_02263 and SETU_00248-00254). The transcriptional upregulation of these transporters may protect the cell from the effects of solute leakage and membrane depolarization caused by sapienic acid (...”
- Inactivation of the Ecs ABC transporter of Staphylococcus aureus attenuates virulence by altering composition and function of bacterial wall
Jonsson, PloS one 2010 - “...1.8 NWMN_0602 iron (chelated) ABC transporter permease protein 1.8 NWMN_0688 ABC transporter ATP-binding protein 0.5 NWMN_0690 ABC transporter 1.9 NWMN_0691 amino acid ABC transporter permease protein 1.9 NWMN_0696 di-/tripeptide ABC transporter 1.9 NWMN_0861 oligopeptide ABC transporter substrate-binding protein 0.4 NWMN_0863 oligopeptide ABC transporter ATP-binding protein 0.2...”
NE1870 potA; putative spermidine/putrescine transport system ATP-binding protein from Nitrosomonas europaea ATCC 19718
41% identity, 59% coverage
EF0674 glycine betaine/carnitine/choline ABC transporter, ATP-binding protein from Enterococcus faecalis V583
40% identity, 59% coverage
BCAL1823 putrescine ABC transporter ATP-binding protein from Burkholderia cenocepacia J2315
38% identity, 61% coverage
BCAS0128 ABC transporter ATP-binding protein from Burkholderia cenocepacia J2315
35% identity, 86% coverage
BCE1437 ABC transporter, ATP-binding protein from Bacillus cereus ATCC 10987
33% identity, 79% coverage
SA0677 hypothetical protein from Staphylococcus aureus subsp. aureus N315
SAV0722 similar to choline transport ATP-binding protein from Staphylococcus aureus subsp. aureus Mu50
41% identity, 58% coverage
- walK and clpP mutations confer reduced vancomycin susceptibility in Staphylococcus aureus
Shoji, Antimicrobial agents and chemotherapy 2011 - “...2.52 2.24 SA0110 SA0111 SA0209 SA0229 SA0639 SA0640 SA0677 SA0891 SA1213 SA1214 SA1979 SA2234 SA2235 SA2236 SA2237 clpP gene SA0723 clpP ATP-dependent Clp...”
- Transcriptome analysis of the responses of Staphylococcus aureus to antimicrobial peptides and characterization of the roles of vraDE and vraSR in antimicrobial resistance
Pietiäinen, BMC genomics 2009 - “...genes homologue SA0513 3.3 2.2 Conserved hypothetical protein SA0591 3.0 3.3 2.1 VCM Hypothetical protein SA0677 3.6 2.1 VCM Similar to choline transport ATP-binding protein SA0781 3.1 2.1 Similar to 2-nitropropane dioxygenase SA0817 5.0 2.7 Similar to NADH-dependent flavin oxidoreductase SA0825 spsA 2.6 3.5 2.0 VraSR,...”
- Transcriptomic and Metabolomic Analysis of a Fusidic Acid-Selected fusA Mutant of Staphylococcus aureus
Gupta, Antibiotics (Basel, Switzerland) 2022 - “...and SAV1381) were upregulated in SH10001st-2, 8 other genes in this category (SACOL1476, SACOL2453, SAR2503, SAV0722, SAS0283, SAV2412, opuCA , and proP ) were downregulated ( Tables S1 and S2 ). At the same time, there were more amino acid biosynthesis genes downregulated ( argF ,...”
OG1RF_RS02205 ABC transporter ATP-binding protein from Enterococcus faecalis OG1RF
40% identity, 59% coverage
- Omadacycline Efficacy against Enterococcus faecalis Isolated in China: In Vitro Activity, Heteroresistance, and Resistance Mechanisms
Lin, Antimicrobial agents and chemotherapy 2020 - “...resistance ( Fig. 2 and Fig. S4). Overexpression of six candidate genes, namely, OG1RF_RS00630, OG1RF_RS12140, OG1RF_RS02205, OG1RF_RS06145, OG1RF_RS06880, and OG1RF_RS11485, was correlated with the occurrence of OMC heteroresistance or resistance ( Fig. 2 ). TABLE 5 Transcriptional expression levels of 12 candidate differentially expressed genes between...”
- “...CoA-binding protein 3.37 (0.0003) 2.67 0.35 OG1RF_RS12140 ABC transporter ATP-binding protein 3.75 (<0.0001) 15.70 2.48 OG1RF_RS02205 ABC transporter ATP-binding protein 3.84 (<0.0001) 3.22 0.49 OG1RF_RS05865 Sulfate ABC transporter ATP-binding protein 4.21 (<0.0001) 8.54 1.24 OG1RF_RS06145 Molybdate ABC transporter substrate-binding protein 5.25 (<0.0001) 21.47 4.53 OG1RF_RS09080 Sugar...”
SO_1271 polyamine ABC transporter ATP-binding protein from Shewanella oneidensis MR-1
SO1271 broad range amino acid ABC transporter, ATP-binding protein from Shewanella oneidensis MR-1
43% identity, 57% coverage
SLUG_20840 ABC transporter ATP-binding protein from Staphylococcus lugdunensis N920143
42% identity, 58% coverage
SAR0775 ABC transporter ATP-binding protein from Staphylococcus aureus subsp. aureus MRSA252
42% identity, 56% coverage
- Global network analysis of drug tolerance, mode of action and virulence in methicillin-resistant S. aureus
Overton, BMC systems biology 2011 - “...SAR1657, SAR1658 (3/3 genes) - 0.027 + Osmoprotectant transport SAR2536-SAR2538 (3/6 genes) 3 genes: SAR2535, SAR0775, SAR0776 0.046 - Virulence factors (ESAT-6 system) SAR0279, SAR0281-0284, SAR0287, SAR0288 (7/12* genes) SAR0285, SAR0286, SAR0289, SAR0290, SAR0291 <1.9E -4 * - High-affinity metal ion transport (Fe) SAR0787-SAR0790 (4/5 genes)...”
- The Staphylococcus aureus response to unsaturated long chain free fatty acids: survival mechanisms and virulence implications
Kenny, PloS one 2009 - “...putative phosphopentomutase 2.45 1.31E-02 SAR0574 putative hexulose-6-phosphate synthase 2.36 1.80E-03 SAR0575 putative 6-phospho-3-hexuloisomerase 2.16 5.11E-03 SAR0775 Osmoprotectant ABC transporter 2.13 4.80E-03 SAR0776 Osmoprotectant ABC transporter, permease protein 2.99 3.00E-04 SAR0824 putative malolactic enzyme 2.59 9.27E-03 SAR0830 tpiA triosephosphate isomerase 2.22 3.39E-02 SAR0831 pgm putative phosphoglycerate mutase...”
Q3J3S3 Spermidine/putrescine import ATP-binding protein PotA from Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.)
42% identity, 65% coverage
SMa1466 Probable ABC transporter ATP binding protein from Sinorhizobium meliloti 1021
36% identity, 86% coverage
SACOL0781 osmoprotectant ABC transporter, ATP-binding protein, putative from Staphylococcus aureus subsp. aureus COL
41% identity, 58% coverage
gbs2088 unknown from Streptococcus agalactiae NEM316
SAK_2068 glycine betaine/carnitine/choline ABC transporter, ATP-binding protein from Streptococcus agalactiae A909
41% identity, 58% coverage
Psyr_4249 Glycine betaine/L-proline transport ATP-binding subunit from Pseudomonas syringae pv. syringae B728a
36% identity, 90% coverage
Q87WH5 ABC-type quaternary amine transporter (EC 7.6.2.9) from Pseudomonas syringae (see paper)
TC 3.A.1.12.10 / Q87WH5 OpuCA aka PSPTO4575, component of The OpuC transporter selective for glycine betaine > choline, acetylcholine, carnitine and proline betaine (contains tandem cystathionine-β-synthase (CBS) domains in the ABC component of OpuC that are required for osmoregulatory function (Chen and Beattie, 2007)) from Pseudomonas syringae pv. tomato (see paper)
PSPTO_4575 glycine betaine/carnitine/choline ABC transporter, ATP-binding protein from Pseudomonas syringae pv. tomato str. DC3000
36% identity, 90% coverage
- substrates: Acetylcholine, Carnitine, Choline, Glycine betaine, Proline betaine
- In planta transcriptomics reveals conflicts between pattern-triggered immunity and the AlgU sigma factor regulon
Wang, PloS one 2022 - “...PSPTO_0129 hemD b TCAGCAGCAGTCTGCCTTTA GTTGCTGAACCCACACTGAA PSPTO_1453 lsc-1 b TCTTTGGTGGTCGTGTAATGG GGTGTGACGCAGGTGTAATAA multiple 16S b ACGGGTACTTGTACCTGGTG CGTTTCCGAGCGTTATCCC PSPTO_4575 opuCA AACCGTCTGATCATGCCGAC TGGATCACGTAGCCGATGTTG PSPTO_1951 fliD TGCAGGGCAAAGGCATTACC TCGTCGAACTGAAGACCAGC PSPTO_4001 avrPto1 TCCAGTCAACTGCTGAGCG TCAGGCTTTGAGGTGCTTGG PSPTO_1243 algD c GAAGAACGGCGACCTGGAACTG CGGTGCTGCGAACCACGATAG a Gene IDs are from GenBank Pto genome NC_004578.1. b hemD , lsc-1 ,...”
- AlgU Controls Expression of Virulence Genes in Pseudomonas syringae pv. tomato DC3000
Markel, Journal of bacteriology 2016 - “...PSPTO_0462 PSPTO_0463 PSPTO_0464 PSPTO_1631 PSPTO_1632 PSPTO_1633 PSPTO_3176 PSPTO_4575 PSPTO_4576 PSPTO_4577 PSPTO_4578 osmC cbcV cbcW cbcX ggnC ggnB asnB lasB...”
- Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function
Chen, Journal of bacteriology 2007 - “...Rfr Spr DC3000 with PSPTO_5273::pKnockout-; Rfr Spr DC3000 with PSPTO_4575; Rfr 41 This work This work This work This work This work Suicide vector for rapid...”
- “...pKnockout- containing 9,018-bp PCR-amplified fragment with PSPTO_4575- PSPTO_4578 E. coli-Pseudomonas shuttle and cloning vector; oriVpVSI oriVp15A oriT; Kmr...”
RSP_2400 ABC putrescine transporter, ATPase subunit from Rhodobacter sphaeroides 2.4.1
46% identity, 55% coverage
- A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales
Alberge, mBio 2023 - “...domain-containing protein P/OM RSP_0251 Q3J1B1 OM efflux protein OM RSP_1033 Q3IZ17 OmpA family protein OM RSP_2400 Q3J3S3 Spermidine/putrescine import ATP-binding protein IM RSP_0240 PntA Q3J1C2 NAD(P) transhydrogenase subunit alpha IM RSP_1199 Q3IYK3 YkuD domain-containing protein (predicted LDT) P/OM RSP_2299 Q3J431 ATP synthase subunit beta 1 IM...”
- The sRNA SorY confers resistance during photooxidative stress by affecting a metabolite transporter in Rhodobacter sphaeroides
Adnan, RNA biology 2015 - “...interaction. The effect of SorY on mRNA levels was confirmed by real time RT-PCR for RSP_2400, RSP_0097, sufC , and adhI ( Fig.2A ). The microarray data for flgF could not be confirmed and RSP_0432 was not tested, since no function for this gene is predicted....”
- “...10 kcal/mol RSP no. Gene Description Ratio [log2 FC] Energy [kcal/mol] Position SorY Position mRNA RSP_2400 ABC putrescine transporter, ATPase subunit 0.76 11.39 35 51 13 2 RSP_0097 takP TRAP-T family transporter, DctP subunit bind prot 0.65 17.61 39 75 44 7 RSP_2401 putative 6-aminohexanoate-cyclic-dimer hydrolase...”
- Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides
Peuser, PloS one 2012 - “...1.00 1.72 1.08 Heme exporter protein D RSP_2399 0.51 0.71 1.73 1.23 ABC putrescine transporter RSP_2400 0.49 0.64 1.74 1.36 ABC putrescine transporter RSP_3571 znuA (2.98) 0.93 (0.26) (1.62) ABC zinc transporter Photo-synthesis RSP_026163 bch (1.17)1.38 2.35 2.73 0.480.53 0.670.85 Chlorophyllide reductase RSP_0277 bchP 0.95 1.81...”
KR75_16575 putrescine ABC transporter ATP-binding subunit PotG from Klebsiella variicola
43% identity, 57% coverage
- Molecular mechanism of endophytic bacteria DX120E regulating polyamine metabolism and promoting plant growth in sugarcane
Qin, Frontiers in plant science 2024 - “...spermidine/putrescine ABC transporter substrate-binding protein 5101970-5102389 KR75_16570 spermidine/putrescine ABC transporter substrate-binding protein PotF potF 3701527-3702639 KR75_16575 putrescine transporter ATP-binding subunit potG 3700230-3701396 KR75_16580 putrescine ABC transporter permease PotH PotH 3699266-3700219 KR75_16585 putrescine ABC transporter permease PotI PotI 3698424-3699269 KR75_17255 gamma-glutamylputrescine oxidoreductase 3536799-3539079 KR75_17260 aldehyde dehydrogenase PuuC...”
PSPTO_2664 spermidine/putrescine ABC transporter, ATP-binding protein from Pseudomonas syringae pv. tomato str. DC3000
43% identity, 56% coverage
AH67_02675 ABC transporter ATP-binding protein from Bifidobacterium pseudolongum PV8-2
41% identity, 59% coverage
SMU_973 ABC transporter ATP-binding protein from Streptococcus mutans UA159
41% identity, 58% coverage
- Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability
Naka, Frontiers in cellular and infection microbiology 2022 - “...system beta-glucoside-specific transporter subunit II 1028324 SMU_980 2.359 potA Spermidine/putrescine ABC transporter ATP-binding protein 1028319 SMU_973 2.272 glnQ Amino acid ABC transporter ATP-binding protein 1028758 SMU_1519 2.233 SMU_567 Glutamine ABC transporter permease 1029364 SMU_567 2.125 SMU_1078c ABC transporter ATP-binding protein 1028403 SMU_1078c 2.039 adcB ABC transporter...”
GALLO_1283 putative proline/glycine betaine ABC transporter ATP-binding protein from Streptococcus gallolyticus UCN34
43% identity, 55% coverage
TM1232 sugar ABC transporter, ATP-binding protein from Thermotoga maritima MSB8
43% identity, 54% coverage
1g291 / Q9YGA6 Malk (see paper)
41% identity, 58% coverage
- Ligands: magnesium ion; pyrophosphate 2- (1g291)
TC 3.A.1.11.7 / Q97Q42 Spermidine/putrescine import ATP-binding protein PotA, component of The spermidine/putrescine uptake porter, PotABCD from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
41% identity, 58% coverage
- substrates: Putrescine, Spermidine
MALK_THELN / Q9YGA6 Trehalose/maltose import ATP-binding protein MalK; EC 7.5.2.1 from Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) (see 5 papers)
41% identity, 58% coverage
- function: Part of the ABC transporter complex MalEFGK involved in trehalose/maltose import. Responsible for energy coupling to the transport system.
catalytic activity: D-maltose(out) + ATP + H2O = D-maltose(in) + ADP + phosphate + H(+) (RHEA:22132)
subunit: Homodimer. The complex is composed of two ATP-binding proteins (MalK), two transmembrane proteins (MalG and MalF) and a solute-binding protein (MalE). - Sugar alcohol degradation in Archaea: uptake and degradation of mannitol and sorbitol in Haloarcula hispanica
Ortjohann, Extremophiles : life under extreme conditions 2024 - “...prediction (AF-G0I055-F1-model_v4). UniProt accession: MscK, G0I055; MtlK, O30494; XacJ, D4GP38; XacK, D4GP39; RhcJ, D4GPB1; Tl-MalK, Q9YGA6; P68187 Mannitol and sorbitol are oxidized to fructose via two distinct dehydrogenases The msc cluster contains two genes, mscS (HAH_5138) and mscM (HAH_5147) , that encode putative dehydrogenases. The functional...”
TC 3.A.1.1.16 / Q8TZQ3 MalK aka PF1933, component of Maltooligosaccharide porter (Maltose is not a substrate, but maltotriose is.) from Pyrococcus furiosus
PF1933 putative sugar transport ATP-hydrolyzing from Pyrococcus furiosus DSM 3638
41% identity, 58% coverage
- substrates: Maltooligosaccharides
- Genome-wide binding analysis of the transcriptional regulator TrmBL1 in Pyrococcus furiosus
Reichelt, BMC genomics 2016 - “...- downstream singleton PF1874 glyceraldehyde-3-phosphate dehydrogenase - upstream singleton PF1920 triosephosphate isomerase - downstream operon PF1933 PF1934 PF1935 PF1936 PF1937 PF1938 putative sugar transport ATP-hydrolyzing hypothetical protein amylopullulanase malG-like sugar transport inner membrane protein malF-like sugar transport inner membrane protein malE-like sugar binding protein MD system...”
- Experimental and computational analysis of the secretome of the hyperthermophilic archaeon Pyrococcus furiosus
Schmid, Extremophiles : life under extreme conditions 2013 - “...4 11 No PF1961 18894163 Tungsten-containing formaldehyde ferredoxin oxidoreductase wor4 69 5 8 No Transport PF1933 18894131 Putative sugar transport ATP-hydrolyzing 41 5 13 No PF1936 18894134 Putative sugar transport inner membrane protein (malg-like) 45 4 12 6 Translation and transcription PF1375 18893486 Translation elongation factor...”
- Evolution of mal ABC transporter operons in the Thermococcales and Thermotogales
Noll, BMC evolutionary biology 2008 - “...the single operons of the former organisms. The mdx operon in P. furiosus (PF1938-1936 and PF1933) encodes a maltodextrin binding protein (MdxE Pf , PF1938) and is upregulated in response to growth on maltose and starch [ 10 , 11 ]. In P. furiosus , the...”
- “...of P. furiosus MalK homologs (ATP binding proteins) . Homologs of the P. furiosus MdxK (PF1933) were gathered using that sequence as query in BLASTP. A ML tree using an alignment of these sequences is shown here. Only bootstrap support values over 800 are shown. For...”
- Impact of substrate glycoside linkage and elemental sulfur on bioenergetics of and hydrogen production by the hyperthermophilic archaeon Pyrococcus furiosus
Chou, Applied and environmental microbiology 2007 - “...tested. It was interesting that the Mal II operon (PF1933 to PF1939) did not respond to glucoside linkage when S0 was present. A similar trend was observed...”
- “...transport, permease Putative sulfate transport, permease Mal II operon PF1933 PF1934 PF1935 PF1936 PF1937 PF1938 PF1939 3.9 8.3 8.4 8.1 NC 9.1 NC NC NC NC NC...”
- Disruption of a sugar transporter gene cluster in a hyperthermophilic archaeon using a host-marker system based on antibiotic resistance
Matsumi, Journal of bacteriology 2007 - “...in Pyrococcus furiosus (Mal-I, PF1739 to PF1744, and Mal-II, PF1933 to PF1938) (33), only one putative gene cluster is found on the T. kodakaraensis genome...”
- Transcriptional and biochemical analysis of starch metabolism in the hyperthermophilic archaeon Pyrococcus furiosus
Lee, Journal of bacteriology 2006 - “...(Mal-I) PF1740 PF1741 PF1742 PF1743 PF1744 PF1933 PF1935*d PF1936 PF1937 PF1938 Maltose/trehalose transport (Mal-I) Maltose/trehalose transport (Mal-I)...”
- “...longer maltooligosaccharides (29). The second transporter (Mal-II; PF1933, PF1936 to PF1938) is specific for maltooligosaccharides, but it does not transport...”
- Whole-genome DNA microarray analysis of a hyperthermophile and an archaeon: Pyrococcus furiosus grown on carbohydrates or peptides
Schut, Journal of bacteriology 2003 - “...transporter (Mal II) cluster in P. furiosus (PF1938 to PF1933) is also up-regulated in maltose-grown cells and is organized in a fashion similar to that of Mal...”
- Biochemical evidence for the presence of two alpha-glucoside ABC-transport systems in the hyperthermophilic archaeon Pyrococcus furiosus
Koning, Archaea (Vancouver, B.C.) 2002 - “...genes encoding the binding protein and two permeases (PF1933, PF1936-PF1938). Instead, two open reading frames (ORFs) separate the second permease and the...”
- “...clusters. Pyrococcus furiosus Escherichia coli MalE PF1938 PF1937 PF1936 PF1933 PF1739 PF1740 PF1741 PF1744 1 2 Identity. Similarity. 1 MalF MalG MalK 2 28 (46)...”
TON_1791 ABC-type maltdextrin transport system, ATPase component from Thermococcus onnurineus NA1
41% identity, 58% coverage
PF1744 trehalose/maltose transport ATP-hydrolyzing from Pyrococcus furiosus DSM 3638
41% identity, 58% coverage
- Evolution of mal ABC transporter operons in the Thermococcales and Thermotogales
Noll, BMC evolutionary biology 2008 - “...starch [ 10 , 11 ]. In P. furiosus , the mal operon (PF1739-1741 and PF1744) encodes MalE Pf , a protein that binds maltose and trehalose, but it does not appear to function as its major maltose transporter [ 10 - 13 ]. The orthologous...”
- Disruption of a sugar transporter gene cluster in a hyperthermophilic archaeon using a host-marker system based on antibiotic resistance
Matsumi, Journal of bacteriology 2007 - “...sugar transporters present in Pyrococcus furiosus (Mal-I, PF1739 to PF1744, and Mal-II, PF1933 to PF1938) (33), only one putative gene cluster is found on the...”
- Impact of substrate glycoside linkage and elemental sulfur on bioenergetics of and hydrogen production by the hyperthermophilic archaeon Pyrococcus furiosus
Chou, Applied and environmental microbiology 2007 - “...Membrane protein, DUF835 Mal I operon PF1739 PF1740 PF1741 PF1742 PF1743 PF1744 PF1745 PF1746 PF1747 PF1748 PF1749 1.6 NC NC 1.7 1.8 1.6 NC NC NC 1.9 NC 2.1 NC...”
- Transcriptional and biochemical analysis of starch metabolism in the hyperthermophilic archaeon Pyrococcus furiosus
Lee, Journal of bacteriology 2006 - “...protein (Mal-I) PF1740 PF1741 PF1742 PF1743 PF1744 PF1933 PF1935*d PF1936 PF1937 PF1938 Maltose/trehalose transport (Mal-I) Maltose/trehalose transport (Mal-I)...”
- “...coli. One is the Mal-I transporter (PF1739 to PF1741, PF1744), which in P. furiosus has been shown to recognize and transport maltose and trehalose but not...”
- Metabolic and evolutionary relationships among Pyrococcus Species: genetic exchange within a hydrothermal vent environment
Hamilton-Brehm, Journal of bacteriology 2005 - “...and malK, represented by PF1739 to PF1741 and PF1744, respectively), as well as a trehalose-degrading enzyme (PF1742) (1, 32, 36, 37, 44, 66). Interestingly,...”
- “...PF0798 PF1337 PF1339 PF1340 PF1737 PF1738 PF1339 PF1740 PF1741 PF1742 PF1743 PF1744 PF1745 PF1746 PF1747 PF1748 PF1749 PF1750 PF1751 * * * * * * * * * * * * *...”
- Biochemical evidence for the presence of two alpha-glucoside ABC-transport systems in the hyperthermophilic archaeon Pyrococcus furiosus
Koning, Archaea (Vancouver, B.C.) 2002 - “...1, Figure 1). One of the clusters (PF1739-PF1741, PF1744) is identical (99-100% amino acid sequence identity) to the trehalose/maltose transport operon found in...”
- “...Escherichia coli MalE PF1938 PF1937 PF1936 PF1933 PF1739 PF1740 PF1741 PF1744 1 2 Identity. Similarity. 1 MalF MalG MalK 2 28 (46) 35 (56) 33 (55) 47 (63) 28...”
MYA_2751 ABC transporter ATP-binding protein from Burkholderia sp. KJ006
37% identity, 62% coverage
D7004_11055 ABC transporter ATP-binding protein from Pedobacter jejuensis
37% identity, 64% coverage
WP_003127477 ABC transporter ATP-binding protein from Enterococcus gallinarum
42% identity, 55% coverage
VK055_4957 ABC transporter ATP-binding protein from Klebsiella pneumoniae subsp. pneumoniae
44% identity, 56% coverage
Atu4786 ABC transporter, nucleotide binding/ATPase protein (iron) from Agrobacterium tumefaciens str. C58 (Cereon)
41% identity, 58% coverage
- Comparative Transcriptome Analysis of Agrobacterium tumefaciens Reveals the Molecular Basis for the Recalcitrant Genetic Transformation of Camellia sinensis L
Jin, Biomolecules 2022 - “...( fbpA = atu0407 , afuA = atu4784 , afuB = atu4785 , afuC = atu4786 and others), maltose ( atu0391 , atu4559 , atu4450 ), oligogalacturonide ( atu3130 , atu3132 ), glucose ( atu3351 , atu3352 ), rhamnose ( atu34873490 ), sn-glycerol 3-phosphate ( ugpC...”
TK1775 ABC-type maltdextrin transport system, ATPase component from Thermococcus kodakaraensis KOD1
40% identity, 58% coverage
lmo0284 similar to ABC transporter (ATP-binding protein) from Listeria monocytogenes EGD-e
45% identity, 57% coverage
Rv2397c PROBABLE SULFATE-TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER CYSA1 from Mycobacterium tuberculosis H37Rv
41% identity, 58% coverage
- Benzene Amide Ether Scaffold is Active against Non-replicating and Intracellular Mycobacterium tuberculosis
Ahmed, ACS infectious diseases 2023 - “...fold change adjusted P -value Rv0560c Rv0560c benzoquinone methyltransferase 8.3 1.6810 58 7.7 3.5210 11 Rv2397c cysA1 sulfate ABC transporter ATP-binding protein 1.2 1.3310 3 0.5 4.0010 1 Rv0848 cysK2 cysteine synthase 4.1 3.3810 27 0.4 9.0010 1 Rv3580c cysS1 cysteine--tRNA ligase 4.2 1.9210 20 0.3...”
- Transcriptional Response of Mycobacterium tuberculosis to Cigarette Smoke Condensate
Willemse, Frontiers in microbiology 2021 - “...Lee et al., 2016 2.63 3.92E-10 MT2067 Rv2012 Conserved hypothetical protein 2.38 1.25E-05 MT2468 * Rv2397c cysA ABC import systems functioning as a sulfate importer Soni et al., 2020 1.01 4.03E-05 MT2469 * Rv2398c cysW Sulfur metabolism Zeng et al., 2013 1.03 2.05E-05 MT2719 Rv2641 cadI...”
- ATP-binding cassette (ABC) import systems of Mycobacterium tuberculosis: target for drug and vaccine development
Soni, Emerging microbes & infections 2020 - “...importers Sulphate importer (SubI-CysT-W-A/Rv2397c-Rv2400c) SBP: SubI (Rv2400c) TMD: CysT (Rv2399c) and CysW (Rv2398c) NBD: CysA (Rv2397c) (two copies) Induced expression of cysT and cysA1 , cysT , cysW , and subI genes in hydrogen peroxide and nutrient starvation conditions, respectively [ 6466 ] Molybdate importer (ModA/Rv1857)...”
- Two Faces of CwlM, an Essential PknB Substrate, in Mycobacterium tuberculosis
Turapov, Cell reports 2018 - “...involved in translation initiation; secretion and membrane proteins EspI ( Zhang etal., 2014 ) and Rv2397c ABC transporter; and conserved proteins of unknown function, Rv2406c and Rv2908. Most proteins had one phosphosite; however, PknB itself, CwlM, and EspI were phosphorylated on several amino acids. Table 1...”
- “...protein AG b IPADVIDAYHAA T 2.53 TrxB1 Rv1471 thioredoxin N AYEVEAGEAT T QNGR 2.45 CysA1 Rv2397c ABC transporter AG GG T EAGNLATSMMK 2.38 EthR Rv3855 transcription repressor N TTSAA S QASLPR 2.29 RpsC Rv0707 ribosomal protein Y AAGGEEAAPDAAAPVEAQSTE S 2.28 FadE10 Rv0873 acyl-CoA de hydrogenase N...”
- Chromosomal rearrangements and protein globularity changes in Mycobacterium tuberculosis isolates from cerebrospinal fluid
Saw, PeerJ 2016 - “...specific substrates; performs chaperone functions in the absence of CLP protease UM-CSF strains N meningitidis Rv2397c cysA1 Probable sulfate-transport ATP-binding protein ABC transporter cysA1 Active transport of multiple sulfur-containing compounds across the cell membrane; energy coupling to the transport system UM-CSF strains Mycobacteria Rv1837c glcB Malate...”
- “...two virulence homologs with this neuropathogen: Rv2457c, encoding ATP-dependent CLP protease ATP-binding subunit clpX and Rv2397c, encoding sulfate-transport ATP-binding protein ABC transporter CysA1. We failed to identify the genes for cell surface outer membrane Opa and Opc proteins that were previously reported to confer tissue tropism...”
- The Mycobacterium tuberculosis Rv2745c plays an important role in responding to redox stress
McGillivray, PloS one 2014 - “...kinase Rv3220c 3.158 3.583 3.231 3.324 10.014 Transport MT2468 cysA1 sulfate ABC transporter, ATP-binding protein Rv2397c 4.467 4.511 3.816 4.265 19.220 MT2470 cysT sulfate ABC transporter, permease protein Rv2399c 2.572 2.203 2.352 2.375 5.189 MT2469 cysW sulfate ABC transporter, permease protein Rv2398c 4.076 3.767 4.115 3.986...”
- “...sensor histidine kinase Rv3220c ND ND ND Transport MT2468 cysA1 sulfate ABC transporter, ATP-binding protein Rv2397c ND ND ND MT2470 cysT sulfate ABC transporter, permease protein Rv2399c ND ND ND MT2469 cysW sulfate ABC transporter, permease protein Rv2398c ND ND ND MT1519 ABC transporter, ATP-binding protein...”
- Identification of a lineage specific zinc responsive genomic island in Mycobacterium avium ssp. paratuberculosis
Eckelt, BMC genomics 2014 - “...- MAP3776c Rv2059 (51.45) MAV_0583 (46.3) <0.0001 13.81 hypothetical protein, putative permease P - MAP3775c Rv2397c (52.38) MAV_0582 (55.5) <0.0001 15.26 ATPase component of Mn/Zn ABC-type transporter P - MAP3774c Rv2060 (37.0) MAV_0581 (51.89) <0.0001 17.58 ABC-type Mn2+/Zn2+ transport system, permease component P - MAP3773c Rv2359...”
- Anti-mycobacterial activity of marine fungus-derived 4-deoxybostrycin and nigrosporin
Wang, Molecules (Basel, Switzerland) 2013 - “...transport and metabolism ctpG Rv1992c 1.60 0.05 Cellular processes: Inorganic ion transport and metabolism cysA Rv2397c 1.78 0.08 Cellular processes: Inorganic ion transport and metabolism Rv0282 - 0.47 0.04 Cellular processes: Posttranslational modification, protein turnover, chaperones Rv3673c - 1.78 0.09 Cellular processes: Posttranslational modification, protein turnover,...”
- More
TC 3.A.1.6.3 / P0A4W2 Sulfate/thiosulfate import ATP-binding protein CysA aka RV2397C aka MT2468 aka MTCY253.24, component of Sulfate porter from Mycobacterium tuberculosis (see 3 papers)
MT2468 sulfate ABC transporter, ATP-binding protein from Mycobacterium tuberculosis CDC1551
41% identity, 58% coverage
- substrates: Sulfate
- Transcriptional Response of Mycobacterium tuberculosis to Cigarette Smoke Condensate
Willemse, Frontiers in microbiology 2021 - “...unknown function Lee et al., 2016 2.63 3.92E-10 MT2067 Rv2012 Conserved hypothetical protein 2.38 1.25E-05 MT2468 * Rv2397c cysA ABC import systems functioning as a sulfate importer Soni et al., 2020 1.01 4.03E-05 MT2469 * Rv2398c cysW Sulfur metabolism Zeng et al., 2013 1.03 2.05E-05 MT2719...”
- The Mycobacterium tuberculosis Rv2745c plays an important role in responding to redox stress
McGillivray, PloS one 2014 - “...5.047 4.952 4.996 31.918 MT3316 sensor histidine kinase Rv3220c 3.158 3.583 3.231 3.324 10.014 Transport MT2468 cysA1 sulfate ABC transporter, ATP-binding protein Rv2397c 4.467 4.511 3.816 4.265 19.220 MT2470 cysT sulfate ABC transporter, permease protein Rv2399c 2.572 2.203 2.352 2.375 5.189 MT2469 cysW sulfate ABC transporter,...”
- “...hypothetical protein Rv1222 ND ND ND MT3316 sensor histidine kinase Rv3220c ND ND ND Transport MT2468 cysA1 sulfate ABC transporter, ATP-binding protein Rv2397c ND ND ND MT2470 cysT sulfate ABC transporter, permease protein Rv2399c ND ND ND MT2469 cysW sulfate ABC transporter, permease protein Rv2398c ND...”
- Reduced immunopathology and mortality despite tissue persistence in a Mycobacterium tuberculosis mutant lacking alternative sigma factor, SigH
Kaushal, Proceedings of the National Academy of Sciences of the United States of America 2002 - “...MT1516, and MT1517 (thioredoxins); MT2063 (ferredoxin); MT2468 and MT2469 (sulfate ABC transporters); MT1163 (citrate synthase); MT2541 (conserved hypothetical...”
Q8DRU8 Quaternary amine transport ATP-binding protein from Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
33% identity, 88% coverage
- Systematic analysis of lysine malonylation in Streptococcus mutans
Li, Frontiers in cellular and infection microbiology 2022 - “...P12655 scrA PTS system sucrose-specific EIIBCA component 46, 601 Q8CVC6 prsA Foldase protein PrsA 253 Q8DRU8 opuCa Putative osmoprotectant amino acid ABC transporter, ATP-binding protein 23 Q8DRV8 SMU_2104 APC family permease(predicted) 472 Q8DS76 SMU_1957 Putative PTS system, mannose-specific IID component 162, 211 Q8DSQ4 SMU_1719c UPF0154 protein...”
EF2652 spermidine/putrescine ABC transporter, ATP-binding subunit from Enterococcus faecalis V583
42% identity, 55% coverage
ABD05_RS05015 ABC transporter ATP-binding protein from Burkholderia pyrrocinia
36% identity, 62% coverage
RHE_RS10605 ABC transporter ATP-binding protein from Rhizobium etli CFN 42
40% identity, 60% coverage
- Rhizobium etli CFN42 proteomes showed isoenzymes in free-living and symbiosis with a different transcriptional regulation inferred from a transcriptional regulatory network
Taboada-Castro, Frontiers in microbiology 2022 - “...K10111 MM RHE_RS27505 malK, mtlK, thuK; multiple sugar transport system ATP-binding protein [EC:7.5.2.-] K10111 MM RHE_RS10605 malK, mtlK, thuK; multiple sugar transport system ATP-binding protein [EC:7.5.2.-] K10111 Bacteroid RHE_RS25965 malK, mtlK, thuK; multiple sugar transport system ATP-binding protein [EC:7.5.2.-] K03406 MM RHE_RS02080 mcp; methyl-accepting chemotaxis protein...”
- “...mtlK , thuK ; multiple sugar transport system ATP-binding protein [EC:3.6.3.-]; in MM, the proteins RHE_RS10605, RHE_RS14795, RHE_RS27505 were identified, and RHE_RS25965 was expressed in bacteroid ( Table 1 ). The msmX , msmK , malK , sugC , ggtA , msiK ; multiple sugar transport...”
YPTB1362 ABC type putrescine/spermidine transporter, ATP-binding protein potG from Yersinia pseudotuberculosis IP 32953
YPO1332 putrescine transport ATP-binding protein from Yersinia pestis CO92
NP_670149 ATP-binding component of putrescine ABC transport system from Yersinia pestis KIM
41% identity, 57% coverage
- Growth of Yersinia pseudotuberculosis in human plasma: impacts on virulence and metabolic gene expression
Rosso, BMC microbiology 2008 - “...hydrolase (pseudogene. stop) 1.578 (< 0.001) 0.669 (0.001) YPTB1352 (sdaC) YPO1321 serine transporteR 0.526 (0.028) YPTB1362 (potG) YPO1332 putrescine transport ATP-binding protein 0.728 (0.035) YPTB1375 (artM) YPO1349 arginine transport system permease protein 1.648 (0.014) 0.572 (0.007) YPTB1384 (poxB) YPO1358 pyruvate dehydrogenase [cytochrome] 1.521 (0.009) YPTB1411 (ansB)...”
- Investigation of and Response to 2 Plague Cases, Yosemite National Park, California, USA, 2015
Danforth, Emerging infectious diseases 2016 - “...YPO0894 980089 15-bp VNTR Gain YPO0968 1072143 SNP C T YPO0976 1084232 SNP G T YPO1332 1498571 SNP T C YPO1422 1617725 18-bp VNTR Loss YPO1705 1946021 SNP C T YPO2153 2423508 SNP C G YPO2253 2531428 SNP T A YPO2556 2871852 6-bp VNTR Gain YPO2840...”
- Growth of Yersinia pseudotuberculosis in human plasma: impacts on virulence and metabolic gene expression
Rosso, BMC microbiology 2008 - “...stop) 1.578 (< 0.001) 0.669 (0.001) YPTB1352 (sdaC) YPO1321 serine transporteR 0.526 (0.028) YPTB1362 (potG) YPO1332 putrescine transport ATP-binding protein 0.728 (0.035) YPTB1375 (artM) YPO1349 arginine transport system permease protein 1.648 (0.014) 0.572 (0.007) YPTB1384 (poxB) YPO1358 pyruvate dehydrogenase [cytochrome] 1.521 (0.009) YPTB1411 (ansB) YPO1386 putative...”
- Extensive antibody cross-reactivity among infectious gram-negative bacteria revealed by proteome microarray analysis
Keasey, Molecular & cellular proteomics : MCP 2009 - “...NP_670026 + 2806 Leucyl, phenylalanyl-tRNA-protein transferase, NP_670106 + + 2849 ATP-binding component of putrescine ABC, NP_670149 + + 2882 pH 6 antigen fimbrial subunit, NP_670182 + + 3096 Hypothetical protein, NP_670395 + + 3140 2,3,4,5-Tetrahydropyridine-2-carboxylate N -succinyltransferase, NP_404654 + + 3504 Suppressor of ftsI, NP_670801 +...”
SEQ_1129 spermidine/putrescine ABC transporter ATP-binding protein from Streptococcus equi subsp. equi 4047
40% identity, 62% coverage
AT5A_10100 ABC transporter ATP-binding protein from Agrobacterium tumefaciens 5A
42% identity, 57% coverage
- Introducing the ArsR-Regulated Arsenic Stimulon
Rawle, Frontiers in microbiology 2021 - “...2.6 AT5A_09690 HmuV Hemin import ATP-binding protein 3.8 AT5A_09695 Hemin ABC transporter transmembrane protein 6.7 AT5A_10100 FbpC Fe(3+) ions import ATP-binding protein 2.4 5.1 4.6 2.0 AT5A_10110 Iron ABC transporter substrate-binding protein -3.2 3.4 3.3 2.3 7.7 8.8 2.3 AT5A_10115 Iron ABC transporter substrate-binding protein 2.5...”
SERP0385 ABC transporter, ATP-binding protein from Staphylococcus epidermidis RP62A
37% identity, 70% coverage
lp_2352 amino acid ABC transporter, ATP-binding protein from Lactobacillus plantarum WCFS1
43% identity, 57% coverage
TON_0985 ABC-type iron(III) transport system, ATPase component from Thermococcus onnurineus NA1
41% identity, 58% coverage
SAG0244 amino acid ABC transporter, ATP-binding protein from Streptococcus agalactiae 2603V/R
SAK_0303 quaternary amine uptake (QAT) family ABC transporter, ATP-binding protein from Streptococcus agalactiae A909
33% identity, 88% coverage
Z1082 ATP-binding component of putrescine transport system from Escherichia coli O157:H7 EDL933
ECs0935 ATP-binding component of putrescine transport system from Escherichia coli O157:H7 str. Sakai
42% identity, 57% coverage
- Small RNA-binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli
Khan, The EMBO journal 2020 - “...in MG1655 derivative strains. The wildtype strain Z854, the qseF mutant Z1081, the qseE mutant Z1082, and the qseF rapZ double mutant Z1083 were assessed under normal growth (mock) and GlcN6P starvation (NvaFMDP) conditions, respectively. Bacteria were grown and analyzed as described for Fig 1 A....”
- Disruption of rcsB by a duplicated sequence in a curli-producing Escherichia coli O157:H7 results in differential gene expression in relation to biofilm formation, stress responses and metabolism
Sharma, BMC microbiology 2017 - “...transporter periplasmic protein 3.66 0.004 potF Z1081 Putrescine ABC transporter periplasmic-binding protein 4.42 4.0E-04 potG Z1082 Putrescine ABC transporter ATP-binding protein 4.00 9.0E-04 potH Z1083 Putrescine ABC transporter membrane protein 2.98 1.5E-02 potI Z1084 Putrescine ABC transporter membrane 2.92 9.0E-03 lolA Z1237 Periplasmic chaperone for translocation...”
- Characterization of genetic and phenotypic diversity of invasive nontypeable Haemophilus influenzae
Erwin, Infection and immunity 2005 - “...(455) .........................................1995; K. Muhlemann (29) R3042 (Z1082), R3046 (Z1030), R3049 (Z202), R3050 (Z343), R3052 (Z1210), R3055 (Z378),...”
- Physiological Response of Escherichia coli O157:H7 Sakai to Dynamic Changes in Temperature and Water Activity as Experienced during Carcass Chilling
King, Molecular & cellular proteomics : MCP 2016 - “...solutes such as glutamate ( ECs0692 , ECs0693 ), a putrescine transporter ATP-binding subunit ( ECs0935 ) and histidine transport system permease protein ( ECs3192 ). A number of genes involved in DNA metabolism were upregulated, including a DNA damage-inducible gene ( dinJ / ECs0253 ),...”
MSMEG_6521 sulfate/thiosulfate import ATP-binding protein CysA 2 from Mycobacterium smegmatis str. MC2 155
MSMEG_6521 ABC transporter ATP-binding protein from Mycolicibacterium smegmatis MC2 155
42% identity, 54% coverage
GBAA5222 ABC transporter, ATP-binding protein from Bacillus anthracis str. 'Ames Ancestor'
41% identity, 57% coverage
gbs0235 unknown from Streptococcus agalactiae NEM316
Q8E7C0 Quaternary amine transport ATP-binding protein from Streptococcus agalactiae serotype III (strain NEM316)
33% identity, 88% coverage
PotG / b0855 putrescine ABC transporter ATP binding subunit (EC 7.6.2.11; EC 7.6.2.16) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
PotG / P31134 putrescine ABC transporter ATP binding subunit (EC 7.6.2.11) from Escherichia coli (strain K12) (see 2 papers)
POTG_ECOLI / P31134 Putrescine transport ATP-binding protein PotG; EC 7.6.2.16 from Escherichia coli (strain K12) (see 2 papers)
TC 3.A.1.11.2 / P31134 PotG aka B0855, component of Putrescine porter from Escherichia coli (see 4 papers)
b0855 ATP-binding component of putrescine transport system from Escherichia coli str. K-12 substr. MG1655
42% identity, 57% coverage
- function: Part of the ABC transporter complex PotFGHI involved in putrescine uptake (PubMed:23719730, PubMed:8416922). Responsible for energy coupling to the transport system (PubMed:23719730). Imports putrescine for maintenance of the optimal concentration of polyamines necessary for cell growth in the presence of glucose (PubMed:23719730).
catalytic activity: putrescine(out) + ATP + H2O = putrescine(in) + ADP + phosphate + H(+) (RHEA:29995)
subunit: The complex is composed of two ATP-binding proteins (PotG), two transmembrane proteins (PotH and PotI) and a solute-binding protein (PotF). - substrates: Putrescine
- Putrescine Supplementation Limits the Expansion of pks+ Escherichia coli and Tumor Development in the Colon
Oliero, Cancer research communications 2024 - “...) which share a 99% similarity to the sequences from E. coli K12 (UniProt: P31133, P31134, P31135, and P0AFL1). First, the cytotoxic potential of this newly isolated pks+ E. coli strain was evaluated using the megalocytosis assay. At MOI 2.5, 5, and 10, the level of...”
- Characterization of a spermine/spermidine transport system reveals a novel DNA sequence duplication in Neisseria gonorrhoeae
Goytia, FEMS microbiology letters 2015 - “...at the residue level. e PotA (P69874) and PotG (P31134) from E. coli share 33% identities at the residue level. potHI::kan C'potHI) expressing potHI from a...”
- Whole-genome sequencing analysis of two heat-evolved Escherichia coli strains
McGuire, BMC genomics 2023 - “...b0789 cardiolipin synthase B FS 15/413 ybjL b0847 putative transport protein YbjL FS 81/561 potG b0855 putrescine ABC transporter ATP binding subunit FS 92/377 elfG b0941 putative fimbrial-like adhesin protein FS 140/356 narX b1222 sensory histidine kinase NarX FS 586/598 yciE b1257 DUF892 domain-containing protein YciE...”
- Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity
Krysenko, Medical sciences (Basel, Switzerland) 2022 - “...ABC transporter substrate-binding protein PotF (b0854) SpuE (PA0301) SCO5667 Polyamine ABC transporter substrate-binding protein PotG (b0855) SpuF (PA0302) SCO5668 Polyamine ABC-transporter integral membrane protein PotH (b0856) SpuG (PA0303) SCO5669 Polyamine ABC-transporter integral membrane protein PotI (b0857) SpuH (PA0304) SCO5670 -glutamyl-polyamine oxidoreductase PuuB (b1301) PauB3 (PA2776) SCO5671...”
- Polyamine transporters and polyamines increase furfural tolerance during xylose fermentation with ethanologenic Escherichia coli strain LY180
Geddes, Applied and environmental microbiology 2014 - “...3.0 3.1 1.0 ABC transporter, putrescine potF potG potH potI b0854 b0855 b0856 b0857 1.0 1.0 0.8 0.8 0.7 0.7 0.8 0.9 0.9 1.0 1.2 1.3 1.4 1.2 1.1 1.2 ABC...”
- Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium
Cortés-Tolalpa, Microbial cell factories 2014 - “...15.6801 hisP b2306 Lysine/arginine/ornithine ABC transporter/histidine ABC transporter - ATP binding subunit Transport 17.8485 potG b0855 Putrescine ABC transporter - ATP binding subunit Transport 18.0562 artJ b0860 Arginine ABC transporter - periplasmic binding protein Transport 120.3908 gadC b1492 Glutamic acid: 4-aminobutyrate antiporter Transport 28.9528 hisM b2307...”
- Computational prediction of the osmoregulation network in Synechococcus sp. WH8102
Mao, BMC genomics 2010 - “...factor b1126 PotA Putrescine/spermidine transporter b1125 PotB b1124 PotC b1123 PotD b0854 PotF Putrescine transporter b0855 PotG b0856 PotH b0857 PotI P. mari ABX75857 GpgS Glucosylglycerate synthetase ABX75858 GpgP sll0689 NhaS3 Na + /H + antiporter sll0493 KtrA Predominant K + transporter playing a major slr1509...”
- “...P.marina ABX75858 GpgP SYNW2434 P.marina slr0662 SpeA SYNW2359 0.99 PCC6803 sll0045 Sps SYNW2520 0.69 PCC6803 b0855 PotG SYNW1544 0.99 E. coli YP_225044 MtrA SYNW2246 C. glut YP_225045 MtrB SYNW0551 C. glut 1. DA: domain architecture When mapping the template networks onto WH8102 , we noticed that...”
CTN_1296 Sugar ABC transporter, ATP-binding protein from Thermotoga neapolitana DSM 4359
41% identity, 56% coverage
BMEI1727 GLYCINE BETAINE/L-PROLINE TRANSPORT ATP-BINDING PROTEIN PROV from Brucella melitensis 16M
44% identity, 51% coverage
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...BMEI1728, proW BruAb10219 BR0224 BOV_0213 BCAN_A0225 OTCN Choline S S -dependent regulation of yehZYXW ABC BMEI1727, proV BruAb10218 BR0223 BOV_0214 BCAN_A0226 84 OTCN Osmoprotectants, Taurine, Cyanante & Nitrate BP BMEI1737 BruAb10207 BR0211 BOV_0204 BCAN_A0215 OTCN Osmoprotectants, Taurine, Cyanante & Nitrate IM BMEI1739 BruAb10206 BR0213 BOV_0202 BCAN_A0213...”
CV4394 probable ABC transporter ATP-binding protein from Chromobacterium violaceum ATCC 12472
44% identity, 56% coverage
T1E_0341 ABC transporter ATP-binding protein from Pseudomonas putida DOT-T1E
39% identity, 63% coverage
- Insights into the susceptibility of Pseudomonas putida to industrially relevant aromatic hydrocarbons that it can synthesize from sugars
García-Franco, Microbial cell factories 2023 - “...were found in operons related to the transport of amino acids (i.e., T1E_2666, T1E_2667, T1E_3208, T1E_0341, T1E_3315) and potassium (T1E_2369) (Additional file 7 : Table S4). While some transporters were downregulated in the presence of trans- cinnamic acid (i.e., T1E_1254, T1E_3944, T1E_0320, T1E_0710, etc.), this was...”
SMDB11_RS03340 methionine ABC transporter ATP-binding protein from Serratia marcescens subsp. marcescens Db11
43% identity, 58% coverage
- RNA‑seq analyses of antibiotic resistance mechanisms in Serratia marcescens
Li, Molecular medicine reports 2019 - “...0.028103517 SMDB11_RS02545 13 SMDB11_RS01845 915.4805 SMDB11_RS02550 0.028097836 SMDB11_RS14100 13 SMDB11_RS05590 890.28687 SMDB11_RS00615 0.028080808 SMDB11_RS14095 12 SMDB11_RS03340 874.0195 SMDB11_RS00620 0.028075136 SMDB11_RS22005 10 SMDB11_RS14915 855.25476 SMDB11_RS00600 0.028075136 SMDB11_RS22020 10 SMDB11_RS02545 767.4091 SMDB11_RS02555 0.028075136 SMDB11_RS14105 10 SMDB11_RS00590 710.21094 SMDB11_RS15030 0.028058134 DEGs, differentially expressed genes....”
BPSS0466 putrescine ABC transport system, ATP-binding protein from Burkholderia pseudomallei K96243
38% identity, 61% coverage
SE0502 choline transport ATP-binding protein from Staphylococcus epidermidis ATCC 12228
37% identity, 70% coverage
SAK_1196 spermidine/putrescine ABC transporter, ATP-binding protein from Streptococcus agalactiae A909
SAG1111 spermidine/putrescine ABC transporter, ATP-binding protein from Streptococcus agalactiae 2603V/R
gbs1178 Unknown from Streptococcus agalactiae NEM316
40% identity, 58% coverage
- Investigation of the polyamine biosynthetic and transport capability of Streptococcus agalactiae: the non-essential PotABCD transporter
Khazaal, Microbiology (Reading, England) 2021 - “...neonate in 1934 (ST 7, CC 7) [ 40 ] A909 potABCD Isogenic potApotBpotCpotD ( sak_1196, sak_1195, sak_1194 and sak_1193 ) deletion mutant of A909 This study Plamids pG+host1 TS Replication-thermosensitive shuttle plasmid, Ery R [ 48 ] Liquid chemically defined media for growth of S....”
- Adaptive response of Group B streptococcus to high glucose conditions: new insights on the CovRS regulation network
Di, PloS one 2013 - “...histidine ABC transporter ( sag0947 to sag0949 ); a spermidine-putrescine transporter, potABCD ( sag1108 to sag1111 ), implicated in the pathogenesis of Streptococcus pneumoniae [29] ; the sag0290 to sag0292 operon (regulated up to 8-fold), encoding a putative polar amino acid ABC transporter; the sag1145 gene,...”
- CsrRS regulates group B Streptococcus virulence gene expression in response to environmental pH: a new perspective on vaccine development
Santi, Journal of bacteriology 2009 - “...SAG0949); a spermidine-putrescine transporter, PotABCD (SAG1108 to SAG1111); and the highly regulated SAG0290-to-SAG0292 operon (11-fold to 23- fold increased),...”
- A naturally occurring gene amplification leading to sulfonamide and trimethoprim resistance in Streptococcus agalactiae
Brochet, Journal of bacteriology 2008 - “...gbs1171 gbs1172 gbs1173 gbs1174 gbs1175 gbs1176 gbs1177 gbs1178 gbs1179 gbs1180 gbs1181 gbs1182 gbs1183 gbs1184 gbs1185 Description of product VOL. 190, 2008...”
LSEI_2628 ABC-type proline/glycine betaine transport system, ATPase component from Lactobacillus casei ATCC 334
41% identity, 56% coverage
- Effect of periodontal pathogens on the metatranscriptome of a healthy multispecies biofilm model
Frias-Lopez, Journal of bacteriology 2012 - “...LSEI_1207, LSEI_2446, LSEI_2447, LSEI_1261, LSEI_1345, LSEI_2628, LSEI_0308, LSEI_0991, LSEI_0999, LSEI_0704, LSEI_0022, LSEI_1814, and LSEI_2466), and also...”
PA3891 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa PAO1
36% identity, 86% coverage
- The ABC of Biofilm Drug Tolerance: the MerR-Like Regulator BrlR Is an Activator of ABC Transport Systems, with PA1874-77 Contributing to the Tolerance of Pseudomonas aeruginosa Biofilms to Tobramycin
Poudyal, Antimicrobial agents and chemotherapy 2018 - “...Probable transporter activity PA3888-91 PA3888 PA3889 PA3890 PA3891 PA4142-44 PA4142 PA4143 PA4144 Protein secretion by the type I secretion system, probable...”
- Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function
Chen, Journal of bacteriology 2007 - “...are available: Psyr4249 in P. syringae pv. syringae B728a, PA3891 in P. aeruginosa PAO1, PP0868 in P. putida KT2440, PSPPH4276 in P. syringae pv. phaseolicola...”
- “...from P. syringae pv. syringae strain B728a and PA3891 from P. aeruginosa PAO1 were functional, as predicted. DISCUSSION The uptake of exogenous compounds that...”
- Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: a transcriptome analysis
Schuster, Journal of bacteriology 2003 - “...PA3678 PA3688 PA3691 PA3692 PA3724 PA3734 PA3888 PA3890 PA3891 PA3904 PA3905 PA3906 PA3907 PA3908 PA3986 PA4078 PA4117 PA4129 PA4130 PA4131 PA4132 PA4133 PA4134...”
Rv3758c POSSIBLE OSMOPROTECTANT (GLYCINE BETAINE/CARNITINE/CHOLINE/L-PROLINE) TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER PROV from Mycobacterium tuberculosis H37Rv
NP_218275 glycine betaine/carnitine/choline/L-proline ABC transporter ATP-binding protein ProV from Mycobacterium tuberculosis H37Rv
42% identity, 59% coverage
- Rifampicin tolerance and growth fitness among isoniazid-resistant clinical Mycobacterium tuberculosis isolates from a longitudinal study
Vijay, eLife 2024 - “...Rv1266c, Rv1696, Rv1758, Rv1997, Rv2043c, Rv2329c, Rv2394, Rv2398c, Rv2400c, Rv2488c, Rv2545, Rv2689c, Rv3138, Rv3680, and Rv3758c previously reported to be associated with persistence, tolerance and survival within host had non-synonymous SNPs associated with changes in rifampicin tolerance ( Figure 7 , Supplementary file 1c with references)....”
- Rifampicin tolerance and growth fitness among isoniazid-resistant clinicalMycobacterium tuberculosisisolates: an in-vitro longitudinal study
Vijay, 2023 - ATP-binding cassette (ABC) import systems of Mycobacterium tuberculosis: target for drug and vaccine development
Soni, Emerging microbes & infections 2020 - “...Glycine betaine importer (ProXVWZ/Rv3759c-Rv3756c) SBP: ProX (Rv3759c) TMD: ProW (Rv3757c), and ProZ (Rv3756c) NBD: ProV (Rv3758c) (two copies) proXVWZ mutant shows decreased survival in macrophages [ 15 ] Import polyphenols instead of glycine betaine [ 32 ] Glutamine importer (Rv2563, GlnQ/Rv2564, Rv0072, Rv0073, and GlnH/Rv0411c) SBP:...”
- Mycobacterium tuberculosis sensor kinase DosS modulates the autophagosome in a DosR-independent manner
Gautam, Communications biology 2019 - “...by phagosomal acidification 25 (Fig. 2n ). On the contrary, the expression of proV ( rv3758c ), which plays a role in the maintenance of osmoregulation within the phagosome 26 , was down-regulated in MtbdosS compared to Mtb (Fig. 2n ). Higher expression of genes involved...”
- In-Vivo Gene Signatures of Mycobacterium tuberculosis in C3HeB/FeJ Mice
Gautam, PloS one 2015 - “...may play a role in DNA supercoiling, macromolecular crowding etc. required during hostile environment. proV (Rv3758c, 2.0 fold induction) mRNA levels were increased in Mtb : dosT infected mouse lungs. proV is involved in osmoregulation as bacteria in the phagosome begin to grow and has been...”
- “...only. In Mtb : dosT both, upregulated (Rv3236c, Rv0311, Rv0465c, Rv2044c etc.) and down-regulated (Rv0045c, Rv3758c, Rv0937c, Rv0060 etc.) genes over 80 days of hypoxia indicate differential gene expression compared to C3HeB/FeJ mouse. The comparative analysis reveals an overlap in many of the genes and their...”
- Development and analysis of an in vivo-compatible metabolic network of Mycobacterium tuberculosis
Fang, BMC systems biology 2010 - “...product of this gene catalyzes the reaction together with that of the TN gene nirB Rv3758c proV Transport Transport of choline, L -carnitine, glycine betaine, and proline The product of this gene catalyzes the transport of choline, L -carnitine, glycine betaine, and proline together with the...”
- In vivo inactivation of the mycobacterial integral membrane stearoyl coenzyme A desaturase DesA3 by a C-terminus-specific degradation process
Chang, Journal of bacteriology 2008 - “...Rv3544c ...................................Acyl-CoA dehydrogenase Rv3758c ...................................Probable ABC transporter Rv3810...”
- Glycine betaine uptake by the ProXVWZ ABC transporter contributes to the ability of Mycobacterium tuberculosis to initiate growth in human macrophages.
Price, Journal of bacteriology 2008 - GeneRIF: role of proXVWZ in allowing M. tuberculosis to obtain betaine from host macrophages and thereby contributes to early steps in colonizing this niche is reported
METN_BACSU / O32169 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Bacillus subtilis (strain 168) (see paper)
TC 3.A.1.24.2 / O32169 MetN (C) (aka YusC), component of The L- and D-methionine porter (also transports methionine sulfoxide from Bacillus subtilis subsp. subtilis str. 168 (see 2 papers)
BSU32750 methionine ABC transporter (ATP-binding protein) from Bacillus subtilis subsp. subtilis str. 168
42% identity, 58% coverage
- function: Part of the ABC transporter complex MetNPQ involved in methionine import (PubMed:14990259). Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in methionine sulfoxide transport (PubMed:14990259).
catalytic activity: L-methionine(out) + ATP + H2O = L-methionine(in) + ADP + phosphate + H(+) (RHEA:29779)
catalytic activity: D-methionine(out) + ATP + H2O = D-methionine(in) + ADP + phosphate + H(+) (RHEA:29767)
subunit: The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetP) and a solute-binding protein (MetQ). - substrates: Methionine, Methionine sulfoxide
- The Blueprint of a Minimal Cell: MiniBacillus
Reuß, Microbiology and molecular biology reviews : MMBR 2016 - “...permease BSU10010 No BSU32730 No 4GOT B. subtilis BSU32740 BSU32750 No No 3DHW 3DHW E. coli E. coli BSU39390 No BSU09130 No Yes BSU36900 Yes Yes BSU09460...”
VC1428 spermidine/putrescine ABC transporter, ATP-binding protein from Vibrio cholerae O1 biovar eltor str. N16961
37% identity, 60% coverage
- Impact of Gene Repression on Biofilm Formation of Vibrio cholerae
Pombo, Frontiers in microbiology 2022 - “...2 VC1373-1375 VC1374 Molecular chaperone DnaK-related protein 1 VC1396-1403 VC1403 Methyl-accepting chemotaxis protein 1 VC1424-1428 VC1428 PotA-putrescine/spermidine ABC transporter ATPase protein 1 VC1433-1434 VC1433 Universal stress protein UspE 1 VC1443 Hypothetical protein 1 VC1444-1445 VC1444 Hypothetical protein 1 VC1445 Sensor histidine kinase/response regulator 1 VC1446-1448 VC1447...”
STM0878 ABC superfamily (atp_bind), putrescine transporter from Salmonella typhimurium LT2
41% identity, 57% coverage
PA14_13580 putative ABC-type proline/glycine betaine transpor from Pseudomonas aeruginosa UCBPP-PA14
36% identity, 86% coverage
- Gene Expression Profiling of <i>Pseudomonas aeruginosa</i> Upon Exposure to Colistin and Tobramycin
Cianciulli, Frontiers in microbiology 2021 - “...protein 4.26 X estA PA14_67510 Esterase EstA 11.29 2.17 ABC transporters and Sha antiporter opuCA PA14_13580 ABC transporter ATP-binding protein 28.83 7.05 opuCB PA14_13590 ABC transporter permease 6 4.83 opuCD PA14_13600 ABC transporter substrate-binding protein 4.73 4.41 nppA2 PA14_41130 ABC transporter substrate-binding protein NppA2 1.97 X...”
- Roles of three transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa choline uptake for catabolism
Malek, Journal of bacteriology 2011 - “...due to the presence of not only OpuC (PA14_13580 to PA14_13600) and CbcVWX (PA14_71000 to PA14_71030), but also three BCCTs, designated BetT1, BetT2, and...”
llmg_1700 choline ABC transporter ATP binding protein from Lactococcus lactis subsp. cremoris MG1363
42% identity, 55% coverage
SG0859 putrescine transport ATP-binding protein PotG from Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
41% identity, 57% coverage
slr1455 sulfate transport system permease protein from Synechocystis sp. PCC 6803
P74548 Sulfate/thiosulfate import ATP-binding protein CysA from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)
40% identity, 60% coverage
- Site-2 Protease Slr1821 Regulates Carbon/Nitrogen Homeostasis during Ammonium Stress Acclimation in Cyanobacterium Synechocystis sp. PCC 6803
Lin, International journal of molecular sciences 2023 - “...), basic amino acid ( slr1735 ), lipopolysaccharide exporter ( slr0251 ), sulfate importer ( slr1455 ) and sodium/glucose cotransporter ( sll1087 ). Lower expression (MA/WTA) was observed in transporters for iron ( slr1295 ), zinc ( slr2045 ), bicarbonate ( slr1512 ), nickel ( sll0381-0383...”
- Ethylene causes transcriptomic changes in Synechocystis during phototaxis
Lacey, Plant direct 2018 - “...to manganese starvation, and Slr1452 that interacts with Slr1453 (also altered by ethylene), Slr1454, and Slr1455 to regulate sulfur uptake. Table 1 Synechocystis gene transcripts upregulated twofold or more by 1l/L ethylene Gene locus number Gene symbol (s) a Description Fold change False discovery rate b...”
- Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803
Pade, Biotechnology for biofuels 2016 - “...sll1249 , phoA ) was downregulated, whereas the sulfate transport operon was induced ( slr1452 slr1455 ). Nitrogen-related genes were also differentially transcribed. The sRNA NsiR4 [ 39 ], the mRNAs nblA1 and nblA2 , and the glutamine synthetase inactivating factors gifA and gifB were upregulated....”
- The nitrogen-regulated response regulator NrrA controls cyanophycin synthesis and glycogen catabolism in the cyanobacterium Synechocystis sp. PCC 6803
Liu, The Journal of biological chemistry 2014 - “...promoter regions of Synechocystis sigE (sll1689) and cysA (slr1455) genes (Fig. 2C). The sigE gene of Synechocystis lacks a predicted NrrA-binding site in the...”
- “...promoter regions of Synechocystis sigE (sll1689) and cysA (slr1455) genes, which lack a putative NrrA-binding site, were used as negative controls. A sequence...”
- Computational prediction of cAMP receptor protein (CRP) binding sites in cyanobacterial genomes
Xu, BMC genomics 2009 - “...slr1839 slr1838 sll1709 slr2082 slr2083 ssl2153 sll0084 slr1349 slr1350 slr1392 sll0537 sll0240 slr1452 slr1453 slr1454 slr1455 slr1457 slr1453 slr1488 sll0273 slr1950 slr1400 slr1805 slr0484 sll1708 sll1371 slr1489 IMS101 Tery_4669 Tery_2879 Tery_1324 Tery_4986 Tery_3858 Tery_0199 Tery_2779 Tery_1627 Tery_3423 Tery_2051 Tery_4912 Tery_1557 BP-1 tsr0033 tlr1171 tll0330 tlr2000 tlr2001...”
- Gene expression patterns of sulfur starvation in Synechocystis sp. PCC 6803
Zhang, BMC genomics 2008 - “...sll1869( cbaB ), Sulfate transport system (slr1452( sbpA ), slr1453( cysT ), slr1454( cysW ), slr1455( cysA )) 6 sll1165( mutS ), slr1511( fabH ), sll1076( ziaA ) 7 sll0306( rpoD ), slr0074( ycf24 ) 9 sll1459, sll1621, slr0143, slr1041, slr0537, sll0856( rpoE ), slr0853( rimI...”
- Comparative analysis of chloroplast genomes: functional annotation, genome-based phylogeny, and deduced evolutionary patterns
De, Genome research 2002 - “...algae 0.1373 sll1833 fts1 0.1373 slr1972 ycf 0.1333 slr1455 cysA 0.1333 slr1453 cysT 0.1064 sll0289 minD 0.1054 slr0772 chlB 0.1054 slr0749 chlL 0.0899 slr0750...”
- Gene discovery within the planctomycete division of the domain Bacteria using sequence tags from genomic DNA libraries
Jenkins, Genome biology 2002 - “...4.00E-05 Listeria monocytogenes (Q56405) B G185 BH001111 Sulfate transport ATP-binding protein CysA 6.00E-35 Synechocystis PCC6803 (P74548) B Regulation and signal transduction G99 BH001112 OmpR homolog 6.00E-11 Guillardia theta chloroplast (AF041468) E(c) G114 BH001113 Nitrogen regulation protein NtrB 2.00E-16 Bradyrhizobium sp.(P10578) B G217 BH001114 Transcriptional regulatory protein...”
BAB1_0224 ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase from Brucella melitensis biovar Abortus 2308
BR0223 ABC transporter, ATP-binding protein from Brucella suis 1330
BOV_0214 ABC transporter, ATP-binding protein from Brucella ovis ATCC 25840
BCAN_A0226 ABC transporter related from Brucella canis ATCC 23365
44% identity, 57% coverage
- Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria
Herrou, Journal of bacteriology 2017 - “...an ATP-binding cassette nucleotide 378 binding domain (bab1_0224), and a small subunit transmembrane permease 379 (bab1_0223) (Figure 1B). The three genes...”
- “...to the YehW transmembrane permease 395 domain (TMD), Bab1_0224 is 49% identical to YehX nucleotide binding domain protein 396 (NBD), Bab1_0225 is 41% identical...”
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...BR0224 BOV_0213 BCAN_A0225 OTCN Choline S S -dependent regulation of yehZYXW ABC BMEI1727, proV BruAb10218 BR0223 BOV_0214 BCAN_A0226 84 OTCN Osmoprotectants, Taurine, Cyanante & Nitrate BP BMEI1737 BruAb10207 BR0211 BOV_0204 BCAN_A0215 OTCN Osmoprotectants, Taurine, Cyanante & Nitrate IM BMEI1739 BruAb10206 BR0213 BOV_0202 BCAN_A0213 OTCN Osmoprotectants, Taurine,...”
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...BOV_0213 BCAN_A0225 OTCN Choline S S -dependent regulation of yehZYXW ABC BMEI1727, proV BruAb10218 BR0223 BOV_0214 BCAN_A0226 84 OTCN Osmoprotectants, Taurine, Cyanante & Nitrate BP BMEI1737 BruAb10207 BR0211 BOV_0204 BCAN_A0215 OTCN Osmoprotectants, Taurine, Cyanante & Nitrate IM BMEI1739 BruAb10206 BR0213 BOV_0202 BCAN_A0213 OTCN Osmoprotectants, Taurine, Cyanante...”
- ATP-Binding Cassette Systems of Brucella
Jenner, Comparative and functional genomics 2009 - “...BCAN_A0225 OTCN Choline S S -dependent regulation of yehZYXW ABC BMEI1727, proV BruAb10218 BR0223 BOV_0214 BCAN_A0226 84 OTCN Osmoprotectants, Taurine, Cyanante & Nitrate BP BMEI1737 BruAb10207 BR0211 BOV_0204 BCAN_A0215 OTCN Osmoprotectants, Taurine, Cyanante & Nitrate IM BMEI1739 BruAb10206 BR0213 BOV_0202 BCAN_A0213 OTCN Osmoprotectants, Taurine, Cyanante &...”
TC 3.A.1.1.22 / Q9X103 MalK; aka Sugar ABC transporter, ATP-binding protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins from Thermotoga maritima (see paper)
TM1276 sugar ABC transporter, ATP-binding protein from Thermotoga maritima MSB8
41% identity, 56% coverage
- substrates: Maltose, Maltotetraose, Maltotriose, Trehalose
- Uncoupling Fermentative Synthesis of Molecular Hydrogen from Biomass Formation in Thermotoga maritima
Singh, Applied and environmental microbiology 2018 - “...type) T. maritima; TM0460 (corresponds to THMB_0470) (W229UGA), TM1276 (corresponds to THMB_1301 of Tma100) (G148D and D345L), 16S rRNA (nucleotide A to G at...”
- “...TM0460 (corresponds to THMC_0470 of Tma200) (W229UGA), TM1276 (corresponds to THMC_1301 of Tma200) (V233S) and 3=TM1322 (corresponds to THMC_1346 of Tma200)...”
- Evolution of mal ABC transporter operons in the Thermococcales and Thermotogales
Noll, BMC evolutionary biology 2008 - “...Fig. 3 is unrelated to any archaeal ORFs. This cluster includes the Tt. maritima ORF TM1276 which, based upon gene expression data, has been suggested to encode a maltose transporter ABP [ 24 , 25 ]. Unfortunately this analysis does not provide additional support for that...”
- “...lack of a malK near these Thermotogales operons is not unusual. Two Tt. maritima ORFs, TM1276 and TM1232, were identified here as potential MalK homologs by phylogenetic analyses, but we did not observe up regulation of either ORF in response to growth on maltose [ 33...”
- An expression-driven approach to the prediction of carbohydrate transport and utilization regulons in the hyperthermophilic bacterium Thermotoga maritima
Conners, Journal of bacteriology 2005 - “...TM0595, TM0596, TM0598 TM0810-TM0812 TM1202-TM1204 TM1232-TM1235 TM1276 TM1836, TM1839 TM1853-TM1855 Unknown This study Unknown This study 47 Unknown...”
Gocc_1613 ABC transporter ATP-binding protein from Gaiella occulta
42% identity, 59% coverage
XAC3669 ABC transporter ATP-binding protein from Xanthomonas axonopodis pv. citri str. 306
45% identity, 54% coverage
SMSK321_1087 ABC transporter ATP-binding protein from Streptococcus mitis SK321
42% identity, 56% coverage
PotA / b1126 spermidine preferential ABC transporter ATP binding subunit (EC 7.6.2.11; EC 7.6.2.16) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
PotA / P69874 spermidine preferential ABC transporter ATP binding subunit (EC 7.6.2.11) from Escherichia coli (strain K12) (see 5 papers)
POTA_ECOLI / P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 6 papers)
TC 3.A.1.11.1 / P69874 Spermidine/putrescine import ATP-binding protein PotA aka B1126, component of Polyamine (putrescine/spermidine) uptake porter from Escherichia coli (see 9 papers)
potA / MB|P69874 spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 from Escherichia coli K12 (see 10 papers)
Z1831 ATP-binding component of spermidine/putrescine transport from Escherichia coli O157:H7 EDL933
NP_415644 spermidine preferential ABC transporter ATP binding subunit from Escherichia coli str. K-12 substr. MG1655
b1126 putrescine/spermidine ABC transporter ATPase protein from Escherichia coli str. K-12 substr. MG1655
S1208 ATP-binding component of spermidine/putrescine transport from Shigella flexneri 2a str. 2457T
41% identity, 58% coverage
- function: Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.
catalytic activity: ATP + H2O + polyamine-[polyamine-binding protein]Side 1 = ADP + phosphate + polyamineSide 2 + [polyamine-binding protein]Side 1.
subunit: The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD). - substrates: Putrescine, Spermidine
tcdb comment: Plays a role in biofilm formation (Zhang et al. 2013). Spermidine-preferring (Igarashi and Kashiwagi 1996) - Comparative genomics analysis and characterization of Shiga toxin-producing Escherichia coli O157:H7 strains reveal virulence genes, resistance genes, prophages and plasmids
Naidoo, BMC genomics 2023 - “...Z1626, Z1723, Z1766, Z1767, Z1768, Z1769, Z1811, Z1812, Z1813, Z1814, Z1815, Z1816, Z1825, Z1826, Z1830, Z1831, Z1832, Z1833, Z1834, Z1835, Z1836. The number or virulence genes present ranged from 15 to 46. The number or pathogen associated genes ranged from 19 to 40. The number or...”
- “...of 50.50%. Fourteen strains (Z852, Z903, Z1626, Z1766, Z1768, Z1769, Z1812, Z1815, Z1816, Z1825, Z1830, Z1831, Z1832 and Z1833) had 21 prophages (13 intact, 5 questionable and 3 incomplete) and percentage GC of 50.53%. The results for the PHASTER analysis are in Additionalfile 2 . Discussion...”
- Identification and functions of amino acid residues in PotB and PotC involved in spermidine uptake activity.
Higashi, The Journal of biological chemistry 2010 - GeneRIF: amino acid residues in PotB and PotC are involved in spermidine uptake activity, and spermidine passage from PotD to the cytoplasm is coupled to the ATPase activity of PotA through a structural change of PotA by its ATPase activity
- Biodistribution of 89Zr-DFO-labeled avian pathogenic Escherichia coli outer membrane vesicles by PET imaging in chickens
Li, Poultry science 2023 - “...Function unknown Cell inner membrane 403 P0ACV0 LPXL Cell wall/membrane/envelope biogenesis Cell inner membrane 404 P69874 POTA Amino acid transport and metabolism Cell inner membrane 405 P17443 MURG Cell wall/membrane/envelope biogenesis Cell inner membrane 406 P11557 DAMX Function unknown Cell inner membrane 407 P0AAE8 CADB Amino...”
- Characterization of a spermine/spermidine transport system reveals a novel DNA sequence duplication in Neisseria gonorrhoeae
Goytia, FEMS microbiology letters 2015 - “...share 16% identities at the residue level. e PotA (P69874) and PotG (P31134) from E. coli share 33% identities at the residue level. potHI::kan C'potHI)...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...polymerase I 5.40/103,118.12 5.37/108,419 PotA P69874 Spermidine/putrescine import ATP-binding protein 5.19/43,028.21 PotD P0AFK9 Spermidine/putrescine-binding...”
- Polyamine transporters and polyamines increase furfural tolerance during xylose fermentation with ethanologenic Escherichia coli strain LY180
Geddes, Applied and environmental microbiology 2014 - “...(15 mM furfural) ABC transporter, spermidine/putrescine potA potB potC potD b1126 b1125 b1124 b1123 0.8 1.1 0.9 0.8 1.0 1.3 1.1 1.3 0.8 0.7 0.5 1.2 2.2 3.0 3.1...”
- Computational prediction of the osmoregulation network in Synechococcus sp. WH8102
Mao, BMC genomics 2010 - “...b2215 OmpC b4111 ProP Betaine/proline transporter b2741 38 RNA polymerase, Sigma 38 (Sigma S) factor b1126 PotA Putrescine/spermidine transporter b1125 PotB b1124 PotC b1123 PotD b0854 PotF Putrescine transporter b0855 PotG b0856 PotH b0857 PotI P. mari ABX75857 GpgS Glucosylglycerate synthetase ABX75858 GpgP sll0689 NhaS3 Na...”
- Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones
Lee, Applied and environmental microbiology 2009 - “...poxB agp potB potA narY narZ b0384 b0405 b0871 b1002 b1125 b1126 b1467 b1468 7.5 4.3 6.1 6.1 4.0 4.6 6.1 8.0 2.5 2.6 1.6 4.9 2.5 2.5 3.2 4.3 Phosphate...”
- Adaptation in bacterial flagellar and motility systems: from regulon members to 'foraging'-like behavior in E. coli
Zhao, Nucleic acids research 2007 - “...Diadenosine tetraphosphatase Salvage of nucleosides and nucleotides 2.5 1.1 b4110 yjcZ Unknown Unknown 2.3 4.2 b1126 potA Spermidine/putrescine transport protein Transport of small molecules: 2.1 1.4 b4315 fimI Fimbrial protein, internal segment Surface structures 2.0 2.6 b0757 galK Galactokinase Degradation of small molecules 2.0 2.6 a...”
- Molecular basis for assembly of the shieldin complex and its implications for NHEJ
Liang, Nature communications 2020 - “...anti-HA (Sigma-Aldrich, H3663) and anti-GFP (Ray Antibody, RM1008). Doxorubicin (also named as Adriamycin, Selleck Chemicals, S1208), Anti-Flag M2 Affinity Gel (Sigma-Aldrich, A2220) and Hoechst 33342 (Biodee, DE0759) were purchased from the indicated sources. Plasmidsare listed in Supplementary Table 2 and Supplementary Table 3 . Cloning cDNAs...”
- Addendum
, Open forum infectious diseases 2019 - Analysis of the type 1 pilin gene cluster fim in Salmonella: its distinct evolutionary histories in the 5' and 3' regions
Boyd, Journal of bacteriology 1999 - “...the fimA and fimI loci; these were strains S1208 (serovar Enteritidis), S1280 (serovar Dublin), and S4993 (serovar Gallinarum). DNA sequencing of PCR-amplified...”
CV_1197 probable ABC-type proline/glycine betaine transport systems, ATPase component from Chromobacterium violaceum ATCC 12472
40% identity, 60% coverage
DSY3918 hypothetical protein from Desulfitobacterium hafniense Y51
38% identity, 75% coverage
Halsa_1783 ABC transporter ATP-binding protein from Halanaerobium hydrogeniformans
40% identity, 56% coverage
WP_048053012 ABC transporter ATP-binding protein from Pyrococcus horikoshii
38% identity, 58% coverage
PFLU1212 putative ABC transport system ATP-binding protein from Pseudomonas fluorescens SBW25
38% identity, 66% coverage
YehX / b2129 glycine betaine ABC transporter ATP binding subunit YehX from Escherichia coli K-12 substr. MG1655 (see 3 papers)
yehX / P33360 glycine betaine ABC transporter ATP binding subunit YehX from Escherichia coli (strain K12) (see 3 papers)
YEHX_ECOLI / P33360 Glycine betaine uptake system ATP-binding protein YehX; EC 7.4.2.- from Escherichia coli (strain K12) (see 2 papers)
b2129 predicted transporter subunit: ATP-binding component of ABC superfamily from Escherichia coli str. K-12 substr. MG1655
41% identity, 61% coverage
- function: Part of an ABC transporter complex involved in low-affinity glycine betaine uptake. Probably responsible for energy coupling to the transport system.
catalytic activity: glycine betaine(out) + ATP + H2O = glycine betaine(in) + ADP + phosphate + H(+) (RHEA:32783)
subunit: The complex is composed of two ATP-binding proteins (YehX), two transmembrane proteins (YehW and YehY) and a solute-binding protein (YehZ). - Data mining of enzymes using specific peptides.
Weingart, BMC bioinformatics 2009 - “...1 P07821 3.6.3 25 25 25 0 3.6.3.34 P0A9V1 3.6.3 7 7 7 0 3.6.3 P33360 3.6.3 13 13 13 0 3.6.3 P76469 4.1.2 9 9 9 0 4.1.2.n3 P77257 3.6.3.17 14 8 8 8 3.6.3 Q81IT9 3.6.1 58 58 58 0 3.6.1 L1 to L4...”
- RpoS-regulated genes of Escherichia coli identified by random lacZ fusion mutagenesis
Vijayakumar, Journal of bacteriology 2004 - “...gabP ugpE b0453 b2663 b3451 ugpC ydcS yehX b3450 b1440 b2129 narY appB IdcC aidB b1467 b0979 b0186 b4187 katE uspB b1732 b3494 osmY b4376 yhiV b3514 yhiU mltB...”
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory