PaperBLAST
PaperBLAST Hits for SwissProt::E4PMA5 Glucosylglycerol phosphorylase; GGoP; 2-O-alpha-D-glucopyranosylglycerol:phosphate alpha-D-glucosyltransferase; 2-O-alpha-D-glucosylglycerol phosphorylase (retaining); EC 2.4.1.359 (Marinobacter adhaerens (strain DSM 23420 / HP15)) (480 a.a., MLLKNAVQLI...)
Show query sequence
>SwissProt::E4PMA5 Glucosylglycerol phosphorylase; GGoP; 2-O-alpha-D-glucopyranosylglycerol:phosphate alpha-D-glucosyltransferase; 2-O-alpha-D-glucosylglycerol phosphorylase (retaining); EC 2.4.1.359 (Marinobacter adhaerens (strain DSM 23420 / HP15))
MLLKNAVQLICYPDRIGNNLKDLYTVVDTHLSEAIGGLHILPFFPSNADGGFSPLTHKEV
DPKVGTWDDIEAFTAKYDLCVDLTVNHISDESPEFTDFIANGFDSEYADLFVHVDKFGEI
SPDDMAKIHIRKEKEPFREVTLSDGTKTRVWCTFTEQQIDLNYESDLAYQLMESYIGFLT
SKGVNLLRLDAFGYTTKRIGTSCFLVEPEVYQILDWVNQVALKHGAECLPEVHDHTSYQY
AISRRNMHPYGFALPPLLLYSLLDANSTYLKNWLRMCPRNMVTVLDTHDGICIPDVEGVL
PDEKIKVLIDNIDARSADPIMRRSAANIHSVGAIYQLTCTFYDALMQNDDAYIAARAIQF
FTPGIPQVYYVGLLAGCNDHELMEQSGELRDINRHYYTLEEVEQDIQKPVVQRLLSLMKF
RSNYPAFDGHFELNYSNNSSVAMAWRHGDYYCHLFVDLNFKTVKVTYTDVETGETRHLEC
Running BLASTp...
Found 229 similar proteins in the literature:
gtfA / E4PMA5 glucosylglycerol phosphorylase (configuration-retaining) (EC 2.4.1.359) from Marinobacter adhaerens (strain DSM 23420 / HP15) (see paper)
GGOP_MARAH / E4PMA5 Glucosylglycerol phosphorylase; GGoP; 2-O-alpha-D-glucopyranosylglycerol:phosphate alpha-D-glucosyltransferase; 2-O-alpha-D-glucosylglycerol phosphorylase (retaining); EC 2.4.1.359 from Marinobacter adhaerens (strain DSM 23420 / HP15) (see paper)
E4PMA5 glucosylglycerol phosphorylase (configuration-retaining) (EC 2.4.1.359) from Marinobacter adhaerens (see paper)
HP15_2853 sucrose phosphorylase from Marinobacter adhaerens HP15
100% identity, 100% coverage
- function: Catalyzes the reversible phosphorolysis of 2-O-alpha-D- glucosylglycerol with retention of the anomeric configuration, forming alpha-D-glucose 1-phosphate and glycerol. Has most likely a catabolic role, either regulating the intracellular levels of glucosylglycerol, which acts as a compatible solute, or degrading it when the environmental conditions change. Cannot catalyze the phosphorolysis of sucrose or glucosylglycerate.
catalytic activity: 2-O-(alpha-D-glucopyranosyl)glycerol + phosphate = alpha-D- glucose 1-phosphate + glycerol (RHEA:56416) - Glucosylglycerol phosphorylase, a potential novel pathway of microbial glucosylglycerol catabolism
Cheng, Applied microbiology and biotechnology 2024 - “...for M. adhaerens HP15, respectively. The locus tag of ggp in M. adhaerens HP15 is HP15_2853 To finely regulate intracellular contents and avoid a net loss of carbon and energy, microbial cells are generally thought to recycle compatible solutes when the stress environment shifts to normal....”
- “...fusion enzyme showing synthase and phosphatase activity as well as a putative hydrolase gene ( HP15_2853 ) that is located downstream of ggpPS (Fig. 1 ) (Luo et al. 2022 ). Considering their predicted functions and genomic organization, we supposed that the hydrolase is involved in...”
- Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases
Li, International journal of molecular sciences 2022 - “...Limosilactobacillus reuteri LTH5448 -Glc1P d -fructose Sucrose [ 41 ] GH13_18 Retaining ADP98617.1 Glucosylglycerol phosphorylase (HP15_2853) Marinobacter adhaerens HP15 -Glc1P d -glycerol Glucosyl-glycerol: (- d -glucopyranosyl-(12)-glycerol) [ 42 ] GH13_18 Retaining ADH62582.1 Glucosylglycerate phosphorylase (MSGGaP;Mesil_0665) Meiothermus silvanus DSM 9946 -Glc1P d -glycerate Glucosyl-glycerate: (- d -glucopyranosyl-(12)-glycerate)...”
7xdqA / E4PMA5 Crystal structure of a glucosylglycerol phosphorylase mutant from marinobacter adhaerens
96% identity, 100% coverage
- Ligand: beta-d-glucopyranose (7xdqA)
FWJ25_14990 sucrose phosphorylase from Marinobacter salinexigens
90% identity, 100% coverage
- Glucosylglycerol phosphorylase, a potential novel pathway of microbial glucosylglycerol catabolism
Cheng, Applied microbiology and biotechnology 2024 - “...same procedure was applied to Marinobacter . Protein expression and purification The coding sequence ( FWJ25_14990 ) of M. salinexigens ZYF650 T GGP was synthesized by BGI (Beijing, China) after codon optimization (supplementary information), and cloned into pET-28b vector with an N-terminal His-tag. To overexpress ggp...”
- “...of GG degradation by GGP To explore the GG-degrading activity, the putative ggp gene ( FWJ25_14990 ) of M. salinexigens ZYF650 T , a deep sea isolate from the Mariana Trench (Ahmad et al. 2020 ), was heterologously expressed in E. coli with an N-terminal His-tag....”
GM661_00120 sucrose phosphorylase from Iocasia fonsfrigidae
44% identity, 99% coverage
- Iocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways
Zhang, Frontiers in microbiology 2021 - “..., and GM661_15065 ) were responsible for the metabolism of cellulose, and the alpha-amylase ( GM661_00120 , GM661_01725 , GM661_02625 , and GM661_03140 ) was responsible for the degradation of starch. In comparison, genes related to the tricarboxylic acid (TCA) cycle are incomplete, indicating the key...”
- “...the other three groups. On the other hand, expressions of genes encoding sucrose phosphorylase ( GM661_00120 ), polysaccharide deacetylase ( GM661_06475 ), peptidoglycan-binding domain-containing protein ( GM661_10570 ), helix-turn-helix domain-containing protein ( GM661_16185 ), and peptidoglycan-binding domain-containing protein ( GM661_16640 ) were markedly increased and these...”
SSU05_1555 Glycosidase from Streptococcus suis 05ZYH33
SSU1369 sucrose phosphorylase from Streptococcus suis P1/7
43% identity, 99% coverage
- The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis
Zhang, Frontiers in cellular and infection microbiology 2017 - “...transporter ATP-binding LOS 2.26 up 5.45E-13 LuxS SSU05_0420 S-ribosyl homocysteinase unknown 2.04 up 1.48E-11 GtfA SSU05_1555 Glycosidase unknown 2.94 up 0 SSU05_0053 Transcriptional regulator unknown 2.17 up 0 Analysis of phosphoproteomics To investigate the Ser/Thr protein kinase activity and the substrates of STK in stk and...”
- GidA, a tRNA Modification Enzyme, Contributes to the Growth, and Virulence of Streptococcus suis Serotype 2
Gao, Frontiers in cellular and infection microbiology 2016 - “...GAPDH SSU05_0155 Glyceraldehyde-3-phosphate dehydrogenase 0.7700 3 90.77 GlnA SSU05_0160 Glutamine synthetase 0.8024 15 48.21 GtfA SSU05_1555 Glycosidase 0.6384 2 6.22 IMPDH SSU05_2183 Inosine 5-monophosphate dehydrogenase 0.6649 1 45.60 PurA SSU05_1966 Adenylosuccinate synthase 0.7722 22 60.23 SadP SSU05_0272 Translation initiation factor 2 GTPase 0.6302 9 18.43 Sly...”
- Genetic diversity of Streptococcus suis isolates as determined by comparative genome hybridization
de, BMC microbiology 2011 - “...36.7 PTS IIABC RD27 SSU1330 - SSU1347 10.041 28 37.1 Phage proteins, hypothetical proteins RD28 SSU1369 - SSU1374 7.733 53 38.8 Sucrose phosphorylase, ABC transporter RD29 SSU1402 - SSU1407 5.018 29 (24) 41.2 Bacitracin export, transposase RD30 SSU1470 - SSU1476 10.163 52 35.4 Two-component regulatory system,...”
SSUBM407_1446 sucrose phosphorylase from Streptococcus suis BM407
43% identity, 99% coverage
- Rapid evolution of virulence and drug resistance in the emerging zoonotic pathogen Streptococcus suis
Holden, PloS one 2009 - “...Region 5 SSUBM407_0840 sensor histidine kinase 742777..744036 37 Region 6 SSUBM407_1445 glucan 1,6-alpha-glucosidase 1533333..1531717 16 SSUBM407_1446 sucrose phosphorylase 1534855..1533407 32 SSUBM407_1447 multiple sugar-binding transport system permease protein 1535755..1534925 33 SSUBM407_1448 multiple sugar-binding transport system permease protein 1536641..1535766 23 SSUBM407_1449 multiple sugar-binding protein precursor 1538008..1536773 16 Region...”
spp / D9TT09 sucrose 6F-phosphate phosphorylase (EC 2.4.1.329) from Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) (see paper)
SUCPP_THETC / D9TT09 Sucrose 6(F)-phosphate phosphorylase; Sucrose 6'-phosphate phosphorylase; SPP; EC 2.4.1.329 from Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) (Clostridium thermosaccharolyticum) (see 3 papers)
D9TT09 sucrose 6F-phosphate phosphorylase (EC 2.4.1.329) from Thermoanaerobacterium thermosaccharolyticum (see paper)
ADL69407.1 6?-P-sucrose phosphorylase (SPP;TtSPP;Tthe_1921) (EC 2.4.1.-) (see protein)
Tthe_1921 sucrose phosphorylase from Thermoanaerobacterium thermosaccharolyticum DSM 571
42% identity, 98% coverage
- function: Catalyzes the reversible phosphorolysis of sucrose 6(F)- phosphate into alpha-D-glucose 1-phosphate (Glc1P) and D-fructose 6- phosphate. May be involved in a new pathway for the degradation of sucrose, which could become phosphorylated on its fructose moiety during uptake via a PTS system. To a lesser extent, can also reversibly act on sucrose in vitro (PubMed:24599311). Is also able to catalyze transglycosylation reactions in vitro (PubMed:26074151).
catalytic activity: sucrose 6(F)-phosphate + phosphate = beta-D-fructose 6- phosphate + alpha-D-glucose 1-phosphate (RHEA:38863)
subunit: Monomer. - Structural Comparison of a Promiscuous and a Highly Specific Sucrose 6F-Phosphate Phosphorylase
Franceus, International journal of molecular sciences 2019 - “...4.3. Gene Cloning and Transformation The T. thermosaccharolyticum sucrose 6 F -phosphate phosphorylase (UniProt code D9TT09) was expressed as described in earlier work [ 18 ]. The amino acid sequence for the I. coccineus phosphorylase (UniProt code M5A566) was codon-optimized for E. coli, synthesized, and subcloned...”
- Chemoenzymatic Approach toward the Synthesis of 3-O-(α/β)-Glucosylated 3-Hydroxy-β-lactams
Decuyper, ACS omega 2018 - “...for mutant R134A of the T. thermosaccharolyticum sucrose 6-phosphate phosphorylase ( TtS PP_R134A; UniProt ID D9TT09), the A. caldus sucrose synthase (SuSy; UniProt ID A0A059ZV61) and the S. rebaudiana UDP-glucosyltransferase (UGT-76G1 Sr ; UniProt ID Q6VAB4) were ligated into expression vectors, transformed in Escherichia coli and...”
- Glucosylglycerate Phosphorylase, an Enzyme with Novel Specificity Involved in Compatible Solute Metabolism
Franceus, Applied and environmental microbiology 2017 - “...T. thermosaccharolyticum sucrose 6=-phosphate phosphorylase (UniProt ID D9TT09). molecule of ATP (3). In vitro, however, sucrose phosphorylase has attracted the...”
- “...D-glyceratea UniProt ID D7BAR0 G0GBS4 P76041 Q84HQ2 P10249 D9TT09 Organism M. silvanus S. thermophila E. coli B. adolescentis S. mutans T. thermosaccharolyticum...”
- Metabolic engineering of Thermoanaerobacterium aotearoense strain SCUT27 for biofuels production from sucrose and molasses
Dai, Biotechnology for biofuels and bioproducts 2023 - “...of the later effective genes, thus ignoring the presence of some useful genes. The gene Tthe_1921 is annotated as sucrose phosphorylase (EC 2.4.1.7), which could degrade sucrose to -D-glucose-1P and fructose [ 29 ]. In addition, Tthe_1923 is annotated as a kind of oligo-1,6-glucosidase [EC:3.2.1.10] with...”
- Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases
Li, International journal of molecular sciences 2022 - “...[ 50 ] GH13_18 ADL69407.1 Thermoanaerobacterium thermosaccharolyticum DSM 571 6S9V[A,B] Sucrose 6(F)-phosphate phosphorylase (SPP; TtSPP; Tthe_1921) (/)8 Monomer [ 50 ] GH65 Q7SIE1 Levilactobacillus brevis ATCC 8287 1H54[A] Maltose phosphorylase (/)6 Dimer [ 197 ] GH65 ADI00307.1 Bacillus selenitireducens MLS10 4KTP[A,B], 4KTR[A,B,C,D,E,F,G,H] 2-O--glucopyranosylglycerol: phosphate -glucosyltransferase/ 2-O--glucosylglycerol...”
A0A1Y3Q6Q6 sucrose phosphorylase (EC 2.4.1.7) from Thermobacillus sp. ZCTH02-B1 (see paper)
44% identity, 98% coverage
AAO21868.1 sucrose phosphorylase (LaSP;GtfA2;LBA1437) (EC 2.4.1.7) (see protein)
LBA1437 sucrose phosphorylase from Lactobacillus acidophilus NCFM
41% identity, 96% coverage
LLKF_2259 sucrose phosphorylase from Lactococcus lactis subsp. lactis KF147
44% identity, 89% coverage
SSA_1006 Dextransucrase, putative from Streptococcus sanguinis SK36
40% identity, 100% coverage
SPCG_RS09680 sucrose phosphorylase from Streptococcus pneumoniae CGSP14
40% identity, 99% coverage
- The transcriptomic response of Streptococcus pneumoniae following exposure to cigarette smoke extract
Manna, Scientific reports 2018 - “...SPCG_RS03585 6-phospho-beta-glucosidase 1.82 0.038 SPCG_RS09705 Alpha-galactosidase Aga 1.72 0.042 SPCG_RS01445 Glutamine-fructose-6-phosphate aminotransferase GlmS 1.54 0.005 SPCG_RS09680 Sucrose phosphorylase GtfA 1.33 0.037 SPCG_RS09470 Galactokinase GalK 1.26 0.007 Vitamin B6 biosynthesis SPCG_RS07540 Pyridoxal 5-phosphate synthase subunit PdxS 1.67 0.004 SPCG_RS07535 Pyridoxal 5-phosphate synthase subunit PdxT 1.41 0.007 Acetolactate...”
SP_1894 sucrose phosphorylase from Streptococcus pneumoniae TIGR4
40% identity, 99% coverage
- The Effect of Impaired Polyamine Transport on Pneumococcal Transcriptome
Nakamya, Pathogens (Basel, Switzerland) 2021 - “...permease SP_2110 malD 2.4 <0.0001 Maltodextrin ABC transporter permease SP_2108 malX 2.4 <0.0001 Maltose/maltodextrin-binding protein SP_1894 gtfA 2.3 <0.0001 Sucrose phosphorylase SP_1722 SP_1722 51.0 <0.0001 PTS sucrose system EIIBCA or EIIBC SP_0648 bgaA 7.0 <0.0001 Beta galactosidase SP_1898 aga 2.2 <0.0001 Alpha galactosidase SP_1721 scrK 10.0...”
- Molecular analysis of an enigmatic Streptococcus pneumoniae virulence factor: The raffinose-family oligosaccharide utilization system
Hobbs, The Journal of biological chemistry 2019 (secret) - Identification of Virulence-Associated Properties by Comparative Genome Analysis of Streptococcus pneumoniae, S. pseudopneumoniae, S. mitis, Three S. oralis Subspecies, and S. infantis
Kilian, mBio 2019 - “...DexS/alpha-phosphotrehalase/alpha-amylase, trehalose PTS system, IIABC components, trehalose operon transcriptional repressor (TreR) 100 100 10 35 SP_1894 Sucrose phosphorylase (GtfA) 100 0 10 76 SP_1895-7 Raffinose ABC transporter (RafG, RafF, RafE); upregulated in contact with lung epithelia ( 13 ) 100 0 0 0 SP_1898-9 Alpha-galactosidase (Aga),...”
- Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
Heß, Nature communications 2017 - “...Table 4 . Beside three variations in intergenic regions, our analysis identified three SNPs in sp_1894 and two SNPs in the pseudogene sp_rs12410 directly located in between tacL and sp_1894 , with a small overlap with the end of the ORF of sp_1894 . However, PCR...”
- “...DNA sequencing of these specific regions disproved two of these SNPs. The confirmed SNPs in sp_1894 , encoding GtfA (a transferase involved in glycosylation of pneumococcal serine-rich repeat adhesins with O -linked N -acetyl-D-glucosamine 24 ), are Ile370Val and a silent one (Glu432Glu). Furthermore, a 1bp...”
spr1709 Dextransucrase (sucrose 6-glucosyltransferase) from Streptococcus pneumoniae R6
40% identity, 99% coverage
- ATTENUATED VACCINE AND DIAGNOSTIC METHOD THEREOF FOR TREATING STREPTOCOCCUS PNEUMONIA USING MUTANT INCLUDING MUTATED SPR1709 GENE
RHEE, 2010
Atu6135 Sucrose phosphorylase-like protein from Agrobacterium tumefaciens str. C58 (Cereon)
41% identity, 93% coverage
Lreu_1542 sucrose phosphorylase from Limosilactobacillus reuteri subsp. reuteri
Lreu_1542 sucrose phosphorylase from Lactobacillus reuteri DSM 20016
40% identity, 93% coverage
LMOf2365_0271 sucrose phosphorylase from Listeria monocytogenes str. 4b F2365
39% identity, 97% coverage
M9ZS93 sucrose phosphorylase (EC 2.4.1.7) from Limosilactobacillus reuteri (see paper)
AGK37834.1 sucrose phosphorylase (ScrP) (EC 2.4.1.7) (see protein)
42% identity, 88% coverage
SUCP_STRMU / P10249 Sucrose phosphorylase; Glucosyltransferase-A; GTF-A; Sucrose glucosyltransferase; EC 2.4.1.7 from Streptococcus mutans serotype c (strain ATCC 700610 / UA159) (see paper)
AAN58596.1 sucrose phosphorylase (GtfA;SMU.881) (EC 2.4.1.7) (see protein)
gtfA sucrose phosphorylase; EC 2.4.1.7 from Streptococcus mutans (see 4 papers)
SMU_881, SMU_RS04095 sucrose phosphorylase from Streptococcus mutans 8ID3
40% identity, 98% coverage
- function: Intracellular catabolism of sucrose (PubMed:2971020). Being intracellular, probably not involved in synthesis of extracellular polysaccharides (Probable).
catalytic activity: sucrose + phosphate = D-fructose + alpha-D-glucose 1- phosphate (RHEA:24048) - Glucosylglycerate Phosphorylase, an Enzyme with Novel Specificity Involved in Compatible Solute Metabolism
Franceus, Applied and environmental microbiology 2017 - “...mesenteroides, UniProt ID Q59495; S. mutans, P10249); empty triangle, T. thermosaccharolyticum sucrose 6=-phosphate phosphorylase (UniProt ID D9TT09). molecule...”
- “...for D-glyceratea UniProt ID D7BAR0 G0GBS4 P76041 Q84HQ2 P10249 D9TT09 Organism M. silvanus S. thermophila E. coli B. adolescentis S. mutans T....”
- Induction of sucrose utilization genes from Bifidobacterium lactis by sucrose and raffinose
Trindade, Applied and environmental microbiology 2003 - “...Streptococcus mutans (level of identity, 41%; accession no. P10249), and Leuconostoc mesenteroides (level of identity, 39%; accession no. Q59495). On the basis...”
- Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability
Naka, Frontiers in cellular and infection microbiology 2022 - “...genes Gene name Description NCBI gene ID Locus tag Fold-change gtfA Sucrose phosphorylase GtfA 1028245 SMU_881 1.438 gbpD Glucan-binding protein D 1028158 SMU_772 1.322 SMU_1039c Lipopolysaccharide glycosyltransferase 1028363 SMU_1039c 1.250 SMU_1434c Glycosyltransferase 1028717 SMU_1434c 1.070 gbpA Glucan-binding protein GbpA 1029286 SMU_2112 1.029 SMU_833 Glycosyltransferase 1029490 SMU_833...”
- Identification of the antibacterial action mechanism of diterpenoids through transcriptome profiling
Moon, Frontiers in microbiology 2022 - “...EIIBC component pttB 2.94 2.56 2.43 SMU_RS04080 Extracellular solute-binding protein msmE 1.23 0.86 2.31 2.26 SMU_RS04095 Sucrose phosphorylase gtfA 0.98 1.69 1.71 SMU_RS04105 Alpha-glucosidase dexB 1.28 1.23 1.25 SMU_RS04075 Alpha-galactosidase aga 1.21 SMU_RS04085 Sugar ABC transporter permease msmF 1.76 0.33 0.45 SMU_RS04090 Carbohydrate ABC transporter permease...”
- Quantitative Proteomics Uncovers the Interaction between a Virulence Factor and Mutanobactin Synthetases in Streptococcus mutans
Rainey, mSphere 2019 - “...Multiple sugar-binding transport ATP-binding protein MsmK 1.68 0.000148 SMU_878 Multiple sugar-binding protein MsmE 1.69 0.001033 SMU_881 Sucrose phosphorylase GtfA 1.52 0.013336 DNA replication/cell division SMU_1967 Single-stranded DNA-binding protein Ssb2 1.60 0.006748 SMU_20 Cell shape-determining protein MreC 2.02 0.001907 SMU_2165 Putative SpoJ, chromosome segregation protein 1.75 0.000467...”
Q59495 sucrose phosphorylase (EC 2.4.1.7) from Leuconostoc mesenteroides (see paper)
BAA14344.1 sucrose phosphorylase (LmSPase) (EC 2.4.1.7) (see protein)
WP_010279952 sucrose phosphorylase from Leuconostoc pseudomesenteroides
40% identity, 97% coverage
2207198A sucrose phosphorylase (EC 2.4.1.7) (see protein)
42% identity, 87% coverage
B8Y3Y0 sucrose phosphorylase (EC 2.4.1.7) from uncultured bacterium (see paper)
39% identity, 95% coverage
AAX33736.1 sucrose phosphorylase (LmSP1) (EC 2.4.1.7) (see protein)
40% identity, 95% coverage
CAA30846.1 sucrose phosphorylase (GftA;SmSP) (EC 2.4.1.7) (see protein)
40% identity, 95% coverage
CAA80424.1 sucrose phosphorylase (EC 2.4.1.7) (see protein)
P33910 Sucrose phosphorylase from Agrobacterium vitis
41% identity, 95% coverage
ABS59292.1 sucrose phosphorylase (742sp) (EC 2.4.1.7) (see protein)
41% identity, 88% coverage
Sfri_3990 sucrose phosphorylase from Shewanella frigidimarina NCIMB 400
40% identity, 97% coverage
- Genomic encyclopedia of sugar utilization pathways in the Shewanella genus
Rodionov, BMC genomics 2010 - “...Shewanella frigidimarina NCIMB400, which contains two divergently transcribed genes, scrT II and scrP (Sfri_3989 and Sfri_3990, respectively) and the intergenic region, was amplified using the primers: 5'-gcgTTATTTAGCGTCTGCGGTCAACAAATG (forward-1) and 5'-gagTTATCCGCTGTGTTTAGCCAGTAAATC (reverse-1). For cloning the fragment containing scrT II only and the intergenic region, the primers of...”
- “...Vermont). Recombinant proteins of S. frigidimarina NCIMB400 ScrT II ( Sfri_3989 ) only, ScrP ( Sfri_3990 ) only, and ScrT II -ScrP were expressed in E. coli K-12 strain DH5 under the control of endogenous promoter in the intergenic region. The empty pBAD-TOPO vector was expressed...”
CHTY_000510 sucrose phosphorylase from Candidatus Phytoplasma meliae
39% identity, 88% coverage
scrP / GI|22550283 sucrose phosphorylase; EC 2.4.1.7 from Bifidobacterium animalis subsp. lactis (see paper)
40% identity, 88% coverage
7znpA Structure of amedsp
40% identity, 95% coverage
- Ligand: magnesium ion (7znpA)
scrP / Q84BY1 sucrose phosphorylase (EC 2.4.1.7) from Bifidobacterium longum (see paper)
AAO84039.1 sucrose phosphorylase (SplP) (EC 2.4.1.7) (see protein)
Q84BY1 ScrP from Bifidobacterium longum
38% identity, 88% coverage
Blon_0128 Sucrose phosphorylase from Bifidobacterium longum subsp. infantis ATCC 15697
38% identity, 88% coverage
- Microbial Glycoside Hydrolases in the First Year of Life: An Analysis Review on Their Presence and Importance in Infant Gut
Ioannou, Frontiers in microbiology 2021 - “...et al., 2013 GH13 Sucrose phosphorylase/inulinase EC 2.4.1.7 Glc1-2Fru B. longum subsp. infantis ATCC 15697 Blon_0128, Blon_1740, Blon_0282, Blon_2453 Kim et al., 2013 *The enzymes for which the gene name is not provided are recorded by their genetic locus. Human milk oligosaccharides are complex structures, and...”
- Proteomic analysis of Bifidobacterium longum subsp. infantis reveals the metabolic insight on consumption of prebiotics and host glycans
Kim, PloS one 2013 - “...used as carbon sources, a specific glycosyl hydrolase (GH) was induced ( Figure 2D ). Blon_0128 is a family 13 GH annotated as a sucrose phosphorylase. These enzymes cleave sucrose to glucose and fructose-6-phosphate. The NSAF of Blon_0128 in the soluble fraction is higher than insoluble...”
- “...activities of the family 32 GH includes inulinase (EC:3.2.1.7), levanase (EC:3.2.1.65), and exo-inulinase (EC:3.2.1.80). While Blon_0128 and Blon_2056 were expressed on both inulin and FOS, the expression of Blon_0787 was observed only in the proteome of FOS grown cells. The localization of Blon_0787 was determined in...”
PIB40_04580 sucrose phosphorylase from Bifidobacterium longum
38% identity, 88% coverage
- Bifidobacterium longum LBUX23 Isolated from Feces of a Newborn; Potential Probiotic Properties and Genomic Characterization
Reyes-Castillo, Microorganisms 2023 - “...such as GalA1 (PIB40_05300), Ldh (PIB40_00085 and PIB40_08485), Ppc (PIB40_04825), GlGP (PIB40_04785), Fba (PIB40_04610), TreY (PIB40_04580), AmyA (PIB40_01230), MalQ1 (PIB40_04535), LeuA (PIB40_04350), and several genes to code by glycosyl hydrolase. These results showed a great diversity of genes and glycosyl-hydrolases, which confer the ability to metabolize...”
BBMN68_1267 sucrose phosphorylase from Bifidobacterium longum subsp. longum BBMN68
39% identity, 88% coverage
- Resistant starch utilization by Bifidobacterium, the beneficial human gut bacteria
Jung, Food science and biotechnology 2023 - “...GH genes encoding sucrose phosphorylase (BBMN68_1267), 4--glucanotransferase (BBMN68_1259 and BBMN68_1607), and pullulanase-like glycosidases (BBMN68_732,...”
- Starch and starch hydrolysates are favorable carbon sources for bifidobacteria in the human gut
Liu, BMC microbiology 2015 - “...to glucose (Figure 3 ). Furthermore, BBMN68 contained genes encoding GH13 family sucrose phosphorylase ( BBMN68_1267 ), 4-alpha-glucanotransferase ( BBMN68_1259, BBMN68_1607 ), which produce glucose 1-phosphate from maltodextrins in starch degradation V pathway [ 16 , 17 ]. Finally, pullulanase-like glycosidases involved in starch degrading [...”
A5A8M5 sucrose phosphorylase (EC 2.4.1.7) from Bifidobacterium longum (see paper)
BAF62433.1 sucrose phosphorylase (Spl) (EC 2.4.1.7) (see protein)
38% identity, 88% coverage
BLLJ_0106 sucrose phosphorylase from Bifidobacterium longum subsp. longum JCM 1217
38% identity, 88% coverage
- Bacterial α-diglucoside metabolism: perspectives and potential for biotechnology and biomedicine
Garcia, Applied microbiology and biotechnology 2021 - “...Franceus and Desmet 2020 ). Briefly, a sucrose phosphorylase from Bifidobacterium longum JCM1217 (locus ID: BLLJ_0106) used phosphate to cleave sucrose and yielded -D-glucose-1-phosphate that was subsequently converted into -D-glucose-6-phosphate by -phosphoglucomutase from Thermococcus kodakarensis KOD1 (locus ID: TK1108). The -D-glucose-6-phosphate was then converted to -D-glucose-1-phosphate...”
AAD40317.1 sucrose phosphorylase (EC 2.4.1.7) (see protein)
38% identity, 93% coverage
BCAL_RS06670 sucrose phosphorylase from Bifidobacterium callitrichos DSM 23973
38% identity, 88% coverage
- The comparative genomics of Bifidobacterium callitrichos reflects dietary carbohydrate utilization within the common marmoset gut
Albert, Microbial genomics 2018 - “...-Glucosidase 3.2.1.20 COO72_RS09510 CPA40_RS01270 BCAL_RS0830 Amylosucrase 2.4.1.4 COO72_RS05900 CPA40_RS04855 BCAL_RS04290 Sucrose phosphorylase 2.4.1.7 COO72_RS10000 CPA40_RS07310 BCAL_RS06670 UTPglucose-1-phosphate uridylyltransferase 2.7.7.9 COO72_RS10475 CPA40_RS09980 BCAL_RS08755 -Amylase 3.2.1.1 COO72_RS11340 CPA40_RS02640 BCAL_RS02400 -Glucosidase 3.2.1.21 COO72_RS09740 CPA40_RS01270 BCAL_RS01445 Isoamylase 3.2.1.68 COO72_RS04240 CPA40_RS05215 BCAL_RS04710 Glucose-1-phosphate adenylyltransferase 2.7.7.27 COO72_RS01125 CPA40_RS05455 BCAL_RS09705 1,4--Glucan branching enzyme...”
AAN24362.1 sucrose phosphorylase (Spl;BL0536) (EC 2.4.1.7) (see protein)
BL0536 sucrose phosphorylase from Bifidobacterium longum NCC2705
Q8G6U7 Sucrose phosphorylase from Bifidobacterium longum (strain NCC 2705)
38% identity, 88% coverage
- Carbohydrate-controlled serine protease inhibitor (serpin) production in Bifidobacterium longum subsp. longum
Duboux, Scientific reports 2021 - “...and a fructose moiety. In contrast to lactose, hydrolysis of sucrose by sucrose phosphorylase ( BL0536 ; EC 2.4.1.7) results in the formation of fructose and glucose-1-phosphate in the cell. Intriguingly, specific regulatory roles have been suggested for intracellular glucose-1-phosphate, including its proposed role in the...”
- Mixed-species genomic microarray analysis of fecal samples reveals differential transcriptional responses of bifidobacteria in breast- and formula-fed infants
Klaassens, Applied and environmental microbiology 2009 - “...tag(s) BAD_0708 BAD_1412 BAD_1527 BAD_1605 BL0146 BL0421 BL0529 BL0536 BL0544 BL0597 BL0715 BL0716 BL0978 BL1104 BL1308 BL1518 BL1638 BL1639 BL1641 BL1643 and...”
- “...of xylans as well. Sucrose phosphorylase (encoded by the BL0536 gene) breaks down sucrose and is involved in energy metabolism. Overall, the expression of the...”
- Energetics of the Glycosyl Transfer Reactions of Sucrose Phosphorylase
Vyas, Biochemistry 2023 - “...Carl Roth or Sigma-Aldrich. Purified preparation of the Bifidobacterium longum sucrose phosphorylase (GeneBank: AAO84039.1; Uniprot: Q8G6U7 ) 48 was used ( Supporting Information , Methods S1.1 and 1.2). Using standard coupled-enzyme assay ( Supporting Information , Methods S1.3.1), the enzyme had a specific activity of 87...”
- “...no competing financial interest. Notes Protein/Enzyme used is Bifidobacterium longum sucrose phosphorylase (GeneBank: AAO84039.1; Uniprot: Q8G6U7 ). Acknowledgments Assistance by Dr. Martin Pfeiffer (protein modeling; docking) and Alexander Sigg (enzyme assays; transglycosylation reactions), both from the Institute of Biotechnology and Biochemical Engineering (TU Graz), is gratefully...”
SUCP_BIFAA / A0ZZH6 Sucrose phosphorylase; SP; SPase; EC 2.4.1.7 from Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) (see 3 papers)
AAO33821.1 sucrose phosphorylase (SucP;SP;BaSP) (EC 2.4.1.7) (see protein)
WP_011742626 sucrose phosphorylase from Bifidobacterium adolescentis
38% identity, 88% coverage
5c8bB / A0ZZH6 Structural insights into the redesign of a sucrose phosphorylase by induced loop repositioning (see paper)
38% identity, 88% coverage
- Ligand: beta-d-glucopyranose (5c8bB)
G4T4R6 sucrose 6F-phosphate phosphorylase (EC 2.4.1.329) from [Ruminococcus] gnavus (see paper)
32% identity, 94% coverage
WP_008399816 sucrose phosphorylase from Clostridium sp. L2-50
29% identity, 85% coverage
- Sucrose 6F-phosphate phosphorylase: a novel insight in the human gut microbiome
Tauzin, Microbial genomics 2019 - “...clinical status. Similarly, the homologous sequences from Blautia (ASM68470.1, 97.8% identity with MH0028_GL0007151) and Clostridium (WP_008399816, 98.4% identity with MH0126_GL0034640) present a relatively high prevalence and are found in 50.9 and 29.7% of individuals, respectively. Moreover, the sequence AAO33821.1 from Bifidobacterium adolescentis DSM 20083 (99.8% identity...”
YM304_32550 glycosidase from Ilumatobacter coccineus YM16-304
30% identity, 90% coverage
- Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases
Li, International journal of molecular sciences 2022 - “...Uncultured bacterium -Glc1P d -fructose Sucrose [ 49 ] GH13_18 Retaining BAN03569.1 Sucrose 6(F)-phosphate phosphorylase (YM304_32550) Ilumatobacter coccineus YM16-304 6S9U -Glc1P d -fructose-6-P Sucrose-6-phosphate [ 50 ] GH65 Inverting AAV43670.1 Maltose phosphorylase (MalP;LBA1870) Lactobacillus acidophilus NCFM -Glc1P d -glucose Maltose: (- d -glucosyl-(14)- d -glucose) [...”
- “...BaSP) (/)8 Dimer [ 196 ] GH13_18 BAN03569.1 Ilumatobacter coccineus YM16-304 6S9U[A] Sucrose 6(F)-phosphate phosphorylase (YM304_32550) (/)8 Monomer [ 50 ] GH13_18 ADL69407.1 Thermoanaerobacterium thermosaccharolyticum DSM 571 6S9V[A,B] Sucrose 6(F)-phosphate phosphorylase (SPP; TtSPP; Tthe_1921) (/)8 Monomer [ 50 ] GH65 Q7SIE1 Levilactobacillus brevis ATCC 8287 1H54[A]...”
SUCPP_ILUCY / A0A6C7EEG6 Sucrose 6(F)-phosphate phosphorylase; SPP; Sucrose 6'-phosphate phosphorylase; EC 2.4.1.329 from Ilumatobacter coccineus (strain NBRC 103263 / KCTC 29153 / YM16-304) (see paper)
A0A6C7EEG6 sucrose 6F-phosphate phosphorylase (EC 2.4.1.329) from Ilumatobacter coccineus (see paper)
30% identity, 89% coverage
- function: Catalyzes the reversible phosphorolysis of sucrose 6(F)- phosphate into alpha-D-glucose 1-phosphate (Glc1P) and D-fructose 6- phosphate (PubMed:31405215). May be involved in a new pathway for the degradation of sucrose, which could become phosphorylated on its fructose moiety during uptake via a PTS system (By similarity). Shows strict specificity since it does not catalyze reactions with alternative substrates (PubMed:31405215).
catalytic activity: sucrose 6(F)-phosphate + phosphate = beta-D-fructose 6- phosphate + alpha-D-glucose 1-phosphate (RHEA:38863)
subunit: Monomer.
6s9uA Crystal structure of sucrose 6f-phosphate phosphorylase from ilumatobacter coccineus (see paper)
30% identity, 89% coverage
- Ligand: phosphate ion (6s9uA)
bhn_I0652 alpha-amylase family glycosyl hydrolase from Butyrivibrio hungatei
28% identity, 83% coverage
SYNPCC7002_A2022 putative sucrose phosphorylase from Synechococcus sp. PCC 7002
28% identity, 70% coverage
LMOf2365_2831 sucrose phosphorylase from Listeria monocytogenes str. 4b F2365
29% identity, 71% coverage
CLL_A2124 putative sucrose phosphorylase from Clostridium botulinum B str. Eklund 17B
30% identity, 75% coverage
- Differentiating Botulinum Neurotoxin-Producing Clostridia with a Simple, Multiplex PCR Assay
Williamson, Applied and environmental microbiology 2017 - “...0.00 0.00 0.00 0.00 CLH_2632 ATP phosphoribosyltransferase regulatory subunit 0.00 0.01 0.99 0.00 0.01 0.01 CLL_A2124 Alpha amylase family protein 0.00 0.00 0.00 0.96 0.00 0.00 a BSR values are a measure of the relatedness of a given coding region sequence between two genomes. Boldface values...”
- “...T258_3337 C. sporogenes ATTGGAGTGGACATGCCTGGA CCCTTTAACCACCGCTTGTTGT 0.5 310 CLH_2632 Group II E AGGGACGCGGGATCTTGTTTTA TCCCATCACTCCCCACTAACTCA 0.4 375 CLL_A2124 Group II BEF GCTTGGACAACATTCAGTGAGGA GAATGCCTTCTGTTGGCCTCAT 1.5 480 16S rRNA gene a CCAGACTCCTACGGGAGGCAG CGTATTACCGCGGCTGCTG 0.05 180 a The 16S rRNA gene primers are from Chakravorty et al. ( 82 ). Isolate...”
AUP74_03226 sugar phosphorylase from Microbulbifer aggregans
29% identity, 70% coverage
- Complete genome sequence of Microbulbifer sp. CCB-MM1, a halophile isolated from Matang Mangrove Forest, Malaysia
Moh, Standards in genomic sciences 2017 - “...No AUP74_00394 Cyclomaltodextrinase Yes AUP74_00399 4-alpha-glucanotransferase No AUP74_00401 Alpha-amylase precursor Yes AUP74_00413 Sucrose phosphorylase No AUP74_03226 16 Glucan endo-1,3-beta-glucosidase A1 precursor No AUP74_01725 Beta-glucanase precursor Yes AUP74_01727 20 N,N-diacetylchitobiase precursor No AUP74_01890 23 Membrane-bound lytic murein transglycosylase F precursor Yes AUP74_00546 Membrane-bound lytic murein transglycosylase F...”
GGAP_ALLS1 / D7BAR0 Glucosylglycerate phosphorylase; GGa phosphorylase; GGaP; EC 2.4.1.352 from Allomeiothermus silvanus (strain ATCC 700542 / DSM 9946 / NBRC 106475 / NCIMB 13440 / VI-R2) (Thermus silvanus) (see paper)
D7BAR0 glucosylglycerate phosphorylase (EC 2.4.1.352) from Meiothermus silvanus (see paper)
30% identity, 75% coverage
- function: Catalyzes the reversible phosphorolysis of glucosylglycerate into alpha-D-glucose 1-phosphate (Glc1P) and D-glycerate. May be a regulator of intracellular levels of glucosylglycerate, a compatible solute that primarily protects organisms facing salt stress and very specific nutritional constraints. Has a very strict substrate specificity. Cannot catalyze the phosphorolysis of sucrose or synthesize sucrose from Glc1P and D-fructose.
catalytic activity: (2R)-2-O-(alpha-D-glucopyranosyl)-glycerate + phosphate = (R)- glycerate + alpha-D-glucose 1-phosphate (RHEA:55268)
GGAP_SPITZ / G0GBS4 Glucosylglycerate phosphorylase; GGa phosphorylase; GGaP; EC 2.4.1.352 from Spirochaeta thermophila (strain ATCC 700085 / DSM 6578 / Z-1203) (see paper)
Spith_0877 sugar phosphorylase from Spirochaeta thermophila DSM 6578
30% identity, 72% coverage
- function: Catalyzes the reversible phosphorolysis of glucosylglycerate into alpha-D-glucose 1-phosphate (Glc1P) and D-glycerate. May be a regulator of intracellular levels of glucosylglycerate, a compatible solute that primarily protects organisms facing salt stress and very specific nutritional constraints. Cannot catalyze the phosphorolysis of sucrose.
catalytic activity: (2R)-2-O-(alpha-D-glucopyranosyl)-glycerate + phosphate = (R)- glycerate + alpha-D-glucose 1-phosphate (RHEA:55268) - Glucosylglycerate Phosphorylase, an Enzyme with Novel Specificity Involved in Compatible Solute Metabolism
Franceus, Applied and environmental microbiology 2017 - “...UniProt identifier [ID] D7BAR0; S. thermophila, G0GBS4; E. coli, P76041); empty squares, Bifidobacterium-like sucrose phosphorylases (B. adolescentis, UniProt...”
- “...from family GH13_18 for D-glyceratea UniProt ID D7BAR0 G0GBS4 P76041 Q84HQ2 P10249 D9TT09 Organism M. silvanus S. thermophila E. coli B. adolescentis S. mutans...”
- Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases
Li, International journal of molecular sciences 2022 - “...Ruminococcus gnavus E1 -Glc1P d -fructose-6-P Sucrose-6-phosphate [ 44 ] GH13_18 Retaining AEJ61152.1 Glucosylglycerate phosphorylase (Spith_0877) Spirochaeta thermophila DSM 6578 -Glc1P d -glycerate Glucosyl-glycerate [ 28 ] GH13_18 Retaining CAA30846.1 Sucrose phosphorylase (GftA;SmSP) Streptococcus mutans INGBRITT/GS5 -Glc1P d -fructose Sucrose [ 45 ] GH13_18 Retaining AAN58596.1...”
BCV53_04180 sugar phosphorylase from Parageobacillus thermoglucosidasius
28% identity, 75% coverage
SYNW2435 putative sucrose phosphorylase from Synechococcus sp. WH 8102
26% identity, 77% coverage
- Computational prediction of the osmoregulation network in Synechococcus sp. WH8102
Mao, BMC genomics 2010 - “...and ProP (SYNW2494), respectively, which are possible candidates for the missing betaine osmolyte transporter subunits. SYNW2435 is added since it shares the same operon with GpgP (SYNW2434), which might be involved in the glucosylglycerate synthesis pathway. SYNW0552 and SYNW2247-2250 are added since they share the same...”
MED152_05075 glycosidase from Polaribacter sp. MED152
29% identity, 55% coverage
Ent638_2165 alpha amylase, catalytic region from Enterobacter sp. 638
28% identity, 71% coverage
- Transcriptional responses to sucrose mimic the plant-associated life style of the plant growth promoting endophyte Enterobacter sp. 638
Taghavi, PloS one 2015 - “...For cultures growing on sucrose, the expression of a putative sucrose phosphorylase gene (EC 2.4.1.7, Ent638_2165, ycjM ) was up-regulated 76 fold after 12 hours compared to 6 hours, pointing towards phosphorolysis as the preferred pathway for sucrose metabolism over hydrolysis by the scrKYAB operon during...”
- “...and S12 represent the condition lactate and sucrose after 6 and 12 hours growth, respectively. Ent638_2165 is a hypothetical gene that showed homology to ycjM , a putative sucrose phosphorylase. Energy metabolism . Among the various pathways of central energy metabolism in Enterobacter sp. 638, the...”
GgaP / b1309 glucosylglycerate phosphorylase (EC 2.4.1.352) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
ycjM / P76041 glucosylglycerate phosphorylase (EC 2.4.1.352) from Escherichia coli (strain K12) (see 4 papers)
GGAP_ECOLI / P76041 Glucosylglycerate phosphorylase; GGa phosphorylase; GGaP; EC 2.4.1.352 from Escherichia coli (strain K12) (see 2 papers)
P76041 glucosylglycerate phosphorylase (EC 2.4.1.352) from Escherichia coli (see paper)
AAC74391.2 sucrose phosphorylase (YcjM) (EC 2.4.1.7) (see protein)
b1309 putative polysaccharide hydrolase from Escherichia coli str. K-12 substr. MG1655
28% identity, 82% coverage
- function: Catalyzes the reversible phosphorolysis of glucosylglycerate into alpha-D-glucose 1-phosphate (Glc1P) and D-glycerate (also called (R)-glycerate) (PubMed:28754708, PubMed:29684280). May be a regulator of intracellular levels of glucosylglycerate, a compatible solute that primarily protects organisms facing salt stress and very specific nutritional constraints (PubMed:28754708). Cannot catalyze the phosphorolysis of sucrose (PubMed:28754708). Does not act on other sugars such as alpha-D-galactose 1-phosphate, alpha-D-mannose 1- phosphate or beta-D-glucose 1-phosphate; in vitro D-erythronate can substitute for D-glycerate with a much lower efficiency (PubMed:29684280).
catalytic activity: (2R)-2-O-(alpha-D-glucopyranosyl)-glycerate + phosphate = (R)- glycerate + alpha-D-glucose 1-phosphate (RHEA:55268)
disruption phenotype: No visible phenotype when grown on glucose. - Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12
Mukherjee, Biochemistry 2018 - “...following set of primers was used to amplify the DNA corresponding to ycjM (gi|90111247; UniProt P76041) from E. coli K-12 MG1655 genomic DNA: 5- AATACTGGATCCATGAAACAGAAAATT -3 5- ATAGTACTCGAGTTATTTAATCCACAT -3 The amplified gene and the pET30a (+) vector were digested with Bam HI and Xho I restriction...”
- Glucosylglycerate Phosphorylase, an Enzyme with Novel Specificity Involved in Compatible Solute Metabolism
Franceus, Applied and environmental microbiology 2017 - “...[ID] D7BAR0; S. thermophila, G0GBS4; E. coli, P76041); empty squares, Bifidobacterium-like sucrose phosphorylases (B. adolescentis, UniProt ID Q84HQ2; B....”
- “...family GH13_18 for D-glyceratea UniProt ID D7BAR0 G0GBS4 P76041 Q84HQ2 P10249 D9TT09 Organism M. silvanus S. thermophila E. coli B. adolescentis S. mutans T....”
- Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ
Gardner, PloS one 2017 - “...component of the ABC superfamily Q363Stop b1234 JW1235 A 1373590 T ycjM predicted glucosyltransferase Q554L b1309 JW1302 C 1966148 G flhA predicted flagellar export pore protein R206P b1879 JW1868 C 2005401 T fliC flagellar filament structural protein E115K b1923 JW1908 C 2045659 A serU tRNA-Ser b1975...”
- 18th Congress of the European Hematology Association, Stockholm, Sweden, June 13–16, 2013
, Haematologica 2013 - Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction
Nichols, Journal of bacteriology 1998 - “...b0940 yccE yceG b1160 fadR ig, kdsA-cha oppC trpC b1309 ydaH recT b1377 trg yddE ig, b1695-b1696 b1754 b1785 ig, b1820-b1821 eda uvrC b1972 ig, yeeF-b2015 gatZ...”
- Biochemistry and regulation of a novel Escherichia coli K-12 porin protein, OmpG, which produces unusually large channels
Fajardo, Journal of bacteriology 1998 - “...encompassing ompG and the psp operon. Ten uncharacterized genes (b1309 to 1317 and orf1) are present between ompG and the psp operon. These 10 ORFs as well...”
lmo2735 similar to Sucrose phosphorylase from Listeria monocytogenes EGD-e
28% identity, 75% coverage
lmo2840 similar to Sucrose phosphorylase from Listeria monocytogenes EGD-e
29% identity, 55% coverage
- Colonisation dynamics of Listeria monocytogenes strains isolated from food production environments
Gray, Scientific reports 2021 - “...lichenan-specific enzyme IIA component; PTS system beta-glucoside-specific IIA component; PTS system cellobiose-specific IIA component 2.7.1.205 lmo2840 fig|1639.4014.peg.393 4.82 YcjM G Sucrose glucosyltransferase/sucrose phosphorylase (ycjM) 2.4.1.7 lmo0034 fig|1639.4014.peg.444 5.61 G PTS system cellobiose-specific IIC component lmo0372 fig|1639.4014.peg.608 4.16 G Glycosyl hydrolase 1 family; 6-phospho-beta-glucosidase 3.2.1.86 lmo0374 fig|1639.4014.peg.610...”
- Prebiotic Oligosaccharides Potentiate Host Protective Responses against L. Monocytogenes Infection
Chen, Pathogens (Basel, Switzerland) 2017 - “..., lmo0347, lmo2696, lmo1057, lmo2648, lmo2836, lmo1463, lmo0877, lmo1182, lmo0383, lmo2101, lmo2749, lmo0372, lmo0917, lmo2771, lmo2840, lmo1915, lmo2362, lmo2095, lmo2835 Secondary metabolite degradation Taxiphyllin bioactivation 0.028 lmo0372, lmo0917, lmo2771 Nitrogen Containing Glucosides Degradation 0.028 Cyanogenic Glucosides Degradation 0.028 Nitrogen Containing Secondary Compounds Degradation 0.039 pathogens-06-00068-t002_Table 2...”
- The ABC transporter AnrAB contributes to the innate resistance of Listeria monocytogenes to nisin, bacitracin, and various beta-lactam antibiotics
Collins, Antimicrobial agents and chemotherapy 2010 - “...lmo2115 (NSL B8; ABC transporter permease), and lmo2840 (NSL G3; similar to sucrose phosphorylase ABC transporter substrate-binding protein). All except lmo2113...”
- “...(), lmo1694 (), lmo1976 (f), lmo2115 (), and lmo2840 (}) mutants (2% inoculum), in TSB-YE containing 200 g/ml Nisaplin. Error bars are standard deviations...”
B8H6N5 amylosucrase (EC 2.4.1.4) from Pseudarthrobacter chlorophenolicus (see 2 papers)
ACL41561.1 amylosucrase (AcAS;Achl_3605) (EC 2.4.1.4) (see protein)
30% identity, 33% coverage
A0A0A0BUC7 amylosucrase (EC 2.4.1.4) from Cellulomonas carbonis (see 3 papers)
29% identity, 30% coverage
A0A0H2X5U3 amylosucrase (EC 2.4.1.4) from Xanthomonas campestris pv. campestris (see paper)
AAM42629.1 sucrose hydrolase (SuxB;XCC3359) (EC 3.2.1.-) (see protein)
Q8P5I2 Amylosucrase or alpha amylase from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
XCC3359 amylosucrase or alpha amylase from Xanthomonas campestris pv. campestris str. ATCC 33913
XC_0805 amylosucrase or alpha amylase from Xanthomonas campestris pv. campestris str. 8004
30% identity, 33% coverage
- Comparative Metagenomics of Cellulose- and Poplar Hydrolysate-Degrading Microcosms from Gut Microflora of the Canadian Beaver (Castor canadensis) and North American Moose (Alces americanus) after Long-Term Enrichment.
Wong, Frontiers in microbiology 2017 - “...(UniProt entry S4XJL8). The structure of DUF3459 has been determined (UniProt entries B2IUW9, Q9RX51, Q8ZPF0, Q8P5I2, Q2PS28, M1E1F6, M1E1F3, H3K096), and as observed here, was previously shown to be part of multi-modular proteins comprising GH13 and CBM48 domains (UniProt entries W6LS46, R4KHQ4, C7RTS8). Although not frequently...”
- Whole-cell bioconversion using non-Leloir transglycosylation reactions: a review
Siziya, Food science and biotechnology 2023 - “...expressed the amylosucrase gene (NCBI-Protein ID: AAM42629; locus XCC3359) from X. campestris pv. campestris str. ATCC 33,913 (NCBI Taxonomy ID: 190485) into a...”
- Community proteogenomics reveals insights into the physiology of phyllosphere bacteria
Delmotte, Proceedings of the National Academy of Sciences of the United States of America 2009 - “...Notably, these genes represent orthologs of XCC3358 and XCC3359. XCC3358 was recently described as one of 72 TonB-dependent receptors in the phytopathogen...”
- Plant carbohydrate scavenging through tonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria
Blanvillain, PloS one 2007 - “...5A ). The XCC3358 gene is likely to form an operon with the downstream gene, XCC3359 ( Figure 6 ). The ortholog of the XCC3359 gene, named suh , has been characterized in Xanthomonas axonopodis pv. glycines ( Xag ), the causal agent of bacterial pustule...”
- “...1, see Materials and Methods and Figure 6 ). A deletion-mutant was also generated into XCC3359 ( XCC3359 1, see Materials and Methods and Figure 6 ). These two mutants showed an altered phenotype, i.e. delayed symptom development, similar to that observed with the pVO155 insertion...”
- Molecular and functional characterization of a unique sucrose hydrolase from Xanthomonas axonopodis pv. glycines
Kim, Journal of bacteriology 2004 - “...of X. campestris pv. campestris bears a structural gene, XCC3359, coding for a protein with a similar length (637 amino acid residues) that also appears...”
- Xanthomonas campestris Promotes Diffusible Signal Factor Biosynthesis and Pathogenicity by Utilizing Glucose and Sucrose from Host Plants
Zhang, Molecular plant-microbe interactions : MPMI 2019 (PubMed)- “...fructose, and in trans expression of one of these enzymes, CINV1 of A. thaliana or XC_0805 of X. campestris pv. campestris, enhanced DSF signal biosynthesis in X. campestris pv. campestris in the presence of sucrose. Taken together, our findings demonstrate that X. campestris pv. campestris applies...”
E2P69_RS15315 alpha-amylase family protein from Xanthomonas perforans
31% identity, 32% coverage
RB5196 alpha-amylase, amylosucrase from Pirellula sp. 1
25% identity, 60% coverage
- Life cycle analysis of the model organism Rhodopirellula baltica SH 1(T) by transcriptome studies
Wecker, Microbial biotechnology 2010 - “...the cell cycle is rather intriguing. The other three regulated genes encoding CAZymes (RB548, RB4894, RB5196) all belong to the family GH13, but have distinct activities. These enzymes are induced in the early or late stationary phase. RB5196 is highly similar to the amylosucrase from Neisseria...”
- Molecular and functional characterization of a unique sucrose hydrolase from Xanthomonas axonopodis pv. glycines
Kim, Journal of bacteriology 2004 - “...to 43% identity) to SUH. These proteins, designated RB5196, CC1135, and DR0933, whose expression and functions have yet to be tested (although their functions...”
- “...AAF10510), a putative Pirellula sp. -amylase or amylosucrase (RB5196; accession number CAD78342), and NpAS (accession number CAA09772). 416 KIM ET AL. that are...”
RB5196 alpha-amylase family glycosyl hydrolase from Rhodopirellula baltica SH 1
25% identity, 63% coverage
CC1135, CC_1135 amylosucrase from Caulobacter crescentus CB15
28% identity, 35% coverage
- A comprehensive genomic, transcriptomic and proteomic analysis of a hyperosmotic stress sensitive α-proteobacterium
Kohler, BMC microbiology 2015 - “...located in the chromosome of C. crescentus . One of these operons is CC_1133 - CC_1135, whose genes code for proteins involved in transport across the bacterial envelope and enzymes acting upon carbohydrates. Upstream of this operon is another gene (CC_1136) involved in transport, which was...”
- Molecular and functional characterization of a unique sucrose hydrolase from Xanthomonas axonopodis pv. glycines
Kim, Journal of bacteriology 2004 - “...43% identity) to SUH. These proteins, designated RB5196, CC1135, and DR0933, whose expression and functions have yet to be tested (although their functions were...”
- “...number AY359289), a putative C. crescentus amylosucrase (CC1135; accession number AAK23119), a putative D. radiodurans -amylase (DR0933; accession number...”
- Identification of long intergenic repeat sequences associated with DNA methylation sites in Caulobacter crescentus and other alpha-proteobacteria
Chen, Journal of bacteriology 2003 - “.... 3351954 3616712 . . . 3616825 CC0375 CC0655 CC0716 CC1135 CC2838 CC2867 CC3011 CC3071 CC3071 CC0562 CC0710 CC1019 CC2224 CC2833 CC3117 CC3367 51 18 54 178 54...”
B6F2G7 amylosucrase (EC 2.4.1.4) from Alteromonas stellipolaris (see paper)
28% identity, 31% coverage
NEISUBOT_05048 alpha-amylase family protein from Neisseria subflava NJ9703
31% identity, 30% coverage
- Versatile biotechnological applications of amylosucrase, a novel glucosyltransferase
Seo, Food science and biotechnology 2020 - “...genes from various microorganisms. Recently, the AS gene from Neisseria subflava ATCC 49275 (gene locus_tag: NEISUBOT_05048, protein ID: EFC51554.1, nsas ) was cloned, based on the sequence of the npas gene and its expression and enzyme characteristics were confirmed (Park et al., 2018a ). Most microbial...”
- “...51.5 Monomer Potocki de Montalk et al. ( 1999 ) NsAS Neisseria subflava ATCC 49275 NEISUBOT_05048 EFC51554.1 45 8.0 N.D N.D Park et al. ( 2018a ) SyAS Synechococcus sp. PCC 7002 FXWN01000001.1 SMQ77851.1 30 6.5 N.D N.D Perez-Cenci and Salerno ( 2014 ) N.D Not...”
AMYS_NEIPO / Q9ZEU2 Amylosucrase; EC 2.4.1.4 from Neisseria polysaccharea (see 7 papers)
Q9ZEU2 amylosucrase (EC 2.4.1.4) from Neisseria polysaccharea (see 19 papers)
AAT15258.1 amylosucrase (Ams;NpAS) (EC 2.4.1.4) (see protein)
30% identity, 32% coverage
- function: Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.
catalytic activity: [(1->4)-alpha-D-glucosyl](n) + sucrose = [(1->4)-alpha-D- glucosyl](n+1) + D-fructose (RHEA:24572)
subunit: Monomer. - Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1
Plaza-Vinuesa, Microbial cell factories 2019 - “...as CSR V [ 10 ]. The sequences from GH13_4 ( Neisseria polysaccharea , accession Q9ZEU2), GH13_16 ( Propionibacterium freudenreichii subsp. Shermanii , A1XGB1), GH13_17 ( Apis mellifera , Q25BT8), GH13_18 ( Bifidobacterium adolescentis , Q84HQ2), GH13_20 ( Lactobacillus plantarum WCFS1, F9URM8), GH13_21 ( Escherichia coli...”
- Replacement of the catalytic nucleophile aspartyl residue of dextran glucosidase by cysteine sulfinate enhances transglycosylation activity
Saburi, The Journal of biological chemistry 2013 - “...Q00250 P00690 P04746 P38940 P10342 P07762 P43379 Q9ZEU2 Neopullulanase Isoamylase Branching enzyme CGTasec Amylosucrase a Bold letters indicate the catalytic...”
3ueqA / Q9ZEU2 Crystal structure of amylosucrase from neisseria polysaccharea in complex with turanose (see paper)
30% identity, 32% coverage
- Ligands: alpha-d-glucopyranose; 3-o-alpha-d-glucopyranosyl-d-fructose (3ueqA)
AAM38333.1 sucrose hydrolase (XAC3490) (EC 3.2.1.-) (see protein)
XAC3490 amylosucrase or alpha amylase from Xanthomonas axonopodis pv. citri str. 306
29% identity, 32% coverage
- A cyclic di-GMP-binding adaptor protein interacts with a N5-glutamine methyltransferase to regulate the pathogenesis in Xanthomonas citri subsp. citri
Shi, Molecular plant pathology 2024 - “...were hrpB2 , hpaA , hrcC , hrpE , avrXacE1 , virK , engXCA , XAC3490 , XAC2853 , gumD , xpsD , xpsE , xpsF and ompW . As expected, RTqPCR analysis revealed significantly decreased mRNA levels of those 14 genes in both N5MapZ R10A...”
- “...including HrpB2 , HpaA , HrcC , HrpE , avrXacE1 , virK , engXCA , XAC3490 , XAC2853 , gumD , xpsD , xpsE , xpsF and ompW in the strain overexpressing N5MapZ R10A or N5MapZ R6AR10A compared to the wild type. The virulence of N5MapZHemK...”
- The Methyltransferase HemK Regulates the Virulence and Nutrient Utilization of the Phytopathogenic Bacterium Xanthomonas citri Subsp. citri
Shi, International journal of molecular sciences 2022 - “..., XAC0346 , XAC0612 , XAC0795 , XAC0933 , XAC2831 , XAC2833 , XAC2853 , XAC3490 and XAC3545 ) ( Figure 6 A, Supplementary Table S1 ). To further confirm whether HemK is required for the regulation of T3SS- and T2SS-associated gene expression, as revealed by...”
- “...and hrpE (T3SS regulators), avrXacE1 , avrBs2 and virK (virulence protein), XAC0612 (cellulase), XAC3545 (protease), XAC3490 (amylosucrase or alpha-amylase) and XAC2853 (cysteine protease). As expected, the qRT-PCR results display a significantly decreased mRNA level of these selected 11 genes in the hemK mutant compared with the...”
- Stringent response regulators (p)ppGpp and DksA positively regulate virulence and host adaptation of Xanthomonas citri
Zhang, Molecular plant pathology 2019 - “...protein (XAC3487), a sugar inner membrane transporter (XAC3488), a TonBdependent transporter (XAC3489) and an amylosucrase (XAC3490) (Fig. 6 B). The suxA , a TonBdependent transporter, suxB and suxC genes were downregulated in both dksA and spoT relA mutants compared to the wildtype Xcc (Fig. 6 B)....”
- Diffusible signal factor (DSF)-mediated quorum sensing modulates expression of diverse traits in Xanthomonas citri and responses of citrus plants to promote disease
Li, BMC genomics 2019 - “...carrier protein XAC3487 cebR 2.20 transcriptional regulator XAC3489 fyuA 1.49 TonB-dependent sucrose outer membrane transporter XAC3490 1.22 amylosucrase or alpha amylase XAC4195 ndvB/celAP 1.23 NdvB protein/ cellobionic acid phosphorylase XAC4355 1.34 Glyco_hydro like XAC4361 ttuB 1.51 MFS transporter Amino acid transport and metabolism XAC0336 metE 1.72...”
- Molecular and functional characterization of a unique sucrose hydrolase from Xanthomonas axonopodis pv. glycines
Kim, Journal of bacteriology 2004 - “...for several segments have corresponding nucleotide sequences in XAC3490, a proteincoding gene found in the genome of X. axonopodis pv. citri. Based on this...”
- “...four nucleotide sequence segments of a proteincoding gene, XAC3490, found in the X. axonopodis pv. citri genome, which has recently been sequenced (7)....”
PXO_RS19450 amylosucrase from Xanthomonas oryzae pv. oryzae PXO99A
31% identity, 32% coverage
- Melatonin Treatment Inhibits the Growth of Xanthomonas oryzae pv. oryzae
Chen, Frontiers in microbiology 2018 - “...Cellulase 37 PXO_RS05615 -3.88 Xylanase 38 PXO_RS01665 -2.32 Xylose isomerase 39 PXO_RS01605 -4.72 Beta-1,4-xylanase 40 PXO_RS19450 +2.54 Glycosidases 41 PXO_RS19890 -2.48 Glycosyl transferase 42 PXO_RS21065 -1.75 Mannose-1-phosphate guanyltransferase 43 PXO_RS23055 +1.61 Fucose permease 44 PXO_RS19900 -2.48 UDP-2,3-diacylglucosamine hydrolase 45 PXO_RS18550 -1.50 Ubiquinol oxidase subunit II 46...”
B6F2H1 amylosucrase (EC 2.4.1.4) from Alteromonas macleodii (see 2 papers)
BAG82876.1 amylosucrase (Amas) (EC 2.4.1.4) (see protein)
28% identity, 30% coverage
3czkA / Q6UVM5 Crystal structure analysis of sucrose hydrolase(suh) e322q-sucrose complex (see paper)
29% identity, 33% coverage
- Ligands: alpha-d-glucopyranose; beta-d-fructofuranose (3czkA)
Q6UVM5 sucrose alpha-glucosidase (EC 3.2.1.48) from Xanthomonas axonopodis (see 2 papers)
AAQ93678.1 sucrose hydrolase (Suh) (EC 3.2.1.-) (see protein)
29% identity, 32% coverage
XHV734_1015 alpha-amylase family protein from Xanthomonas hortorum pv. vitians
29% identity, 32% coverage
D7CVD0 amylosucrase (EC 2.4.1.4) from Truepera radiovictrix (see paper)
28% identity, 32% coverage
ams / G4T024 sucrose β-fructofuranosidase (EC 3.2.1.26; EC 2.4.1.4) from Methylotuvimicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z) (see 4 papers)
G4T024 amylosucrase (EC 2.4.1.4) from Methylotuvimicrobium alcaliphilum (see 3 papers)
25% identity, 61% coverage
Gbem_0136 trehalose synthase from Geobacter bemidjiensis Bem
26% identity, 32% coverage
B1XIU7 amylosucrase (EC 2.4.1.4) from Synechococcus sp. (see paper)
ACA98889.1 amylosucrase (AmsA;1986-orf;AMS;SYNPCC7002_A0885) (EC 2.4.1.4) (see protein)
24% identity, 64% coverage
5x7uA / D1CE96 Trehalose synthase from thermobaculum terrenum (see paper)
25% identity, 75% coverage
- Ligand: magnesium ion (5x7uA)
CPA40_RS04855 alpha-amylase family protein from Bifidobacterium callitrichos
26% identity, 48% coverage
- The comparative genomics of Bifidobacterium callitrichos reflects dietary carbohydrate utilization within the common marmoset gut
Albert, Microbial genomics 2018 - “...CPA40_RS06365 BCAL_RS06200 -Fructofuranosidase 3.2.1.26 COO72_RS02000 CPA40_RS06035 BCAL_RS10725 -Glucosidase 3.2.1.20 COO72_RS09510 CPA40_RS01270 BCAL_RS0830 Amylosucrase 2.4.1.4 COO72_RS05900 CPA40_RS04855 BCAL_RS04290 Sucrose phosphorylase 2.4.1.7 COO72_RS10000 CPA40_RS07310 BCAL_RS06670 UTPglucose-1-phosphate uridylyltransferase 2.7.7.9 COO72_RS10475 CPA40_RS09980 BCAL_RS08755 -Amylase 3.2.1.1 COO72_RS11340 CPA40_RS02640 BCAL_RS02400 -Glucosidase 3.2.1.21 COO72_RS09740 CPA40_RS01270 BCAL_RS01445 Isoamylase 3.2.1.68 COO72_RS04240 CPA40_RS05215 BCAL_RS04710 Glucose-1-phosphate adenylyltransferase...”
BCAL_RS04290 alpha-amylase family protein from Bifidobacterium callitrichos DSM 23973
26% identity, 48% coverage
- The comparative genomics of Bifidobacterium callitrichos reflects dietary carbohydrate utilization within the common marmoset gut
Albert, Microbial genomics 2018 - “...BCAL_RS06200 -Fructofuranosidase 3.2.1.26 COO72_RS02000 CPA40_RS06035 BCAL_RS10725 -Glucosidase 3.2.1.20 COO72_RS09510 CPA40_RS01270 BCAL_RS0830 Amylosucrase 2.4.1.4 COO72_RS05900 CPA40_RS04855 BCAL_RS04290 Sucrose phosphorylase 2.4.1.7 COO72_RS10000 CPA40_RS07310 BCAL_RS06670 UTPglucose-1-phosphate uridylyltransferase 2.7.7.9 COO72_RS10475 CPA40_RS09980 BCAL_RS08755 -Amylase 3.2.1.1 COO72_RS11340 CPA40_RS02640 BCAL_RS02400 -Glucosidase 3.2.1.21 COO72_RS09740 CPA40_RS01270 BCAL_RS01445 Isoamylase 3.2.1.68 COO72_RS04240 CPA40_RS05215 BCAL_RS04710 Glucose-1-phosphate adenylyltransferase 2.7.7.27...”
D1CE96 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Thermobaculum terrenum (see 4 papers)
25% identity, 75% coverage
A0A4P8XUU6 amylosucrase (EC 2.4.1.4) from Deinococcus radiopugnans (see paper)
26% identity, 32% coverage
Q1J0W0 amylosucrase (EC 2.4.1.4) from Deinococcus geothermalis (see 10 papers)
ABF44874.1 amylosucrase (DgAS;Dgeo_0572) (EC 2.4.1.4) (see protein)
26% identity, 32% coverage
3uerA / Q1J0W0 Crystal structure of amylosucrase from deinococcus geothermalis in complex with turanose (see paper)
26% identity, 32% coverage
- Ligands: alpha-d-fructofuranose; alpha-d-glucopyranose; beta-d-fructofuranose (3uerA)
SPV1_05592 Alpha amylase, catalytic region from Mariprofundus ferrooxydans PV-1
26% identity, 31% coverage
Q1J0Z5 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Deinococcus geothermalis (see paper)
26% identity, 48% coverage
I3NX86 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Deinococcus radiodurans (see 4 papers)
24% identity, 62% coverage
Q9RST7 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Deinococcus radiodurans (see 2 papers)
DR2036 trehalose synthase, putative from Deinococcus radiodurans R1
24% identity, 63% coverage
BL105A_1718 alpha-amylase family protein from Bifidobacterium longum
28% identity, 30% coverage
9ezlA / I3NX86 Crystal structure of trehalose synthase mutant n253h from deinococcus radiodurans (see paper)
27% identity, 36% coverage
- Ligands: calcium ion; magnesium ion (9ezlA)
Q9RVT9 amylosucrase (EC 2.4.1.4) from Deinococcus radiodurans (see 2 papers)
AAF10510.1 amylosucrase (AS;DrAS;DR0933) (EC 2.4.1.4) (see protein)
DR0933 alpha-amlyase from Deinococcus radiodurans R1
29% identity, 26% coverage
- A Novel Glycoside Hydrolase DogH Utilizing Soluble Starch to Maltose Improve Osmotic Tolerance in Deinococcus radiodurans
Gui, International journal of molecular sciences 2023 - “...TPP/TPS pathway and produces trehalose mainly through the TreY/TreZ ( dr0463/dr0464 ) and TreS ( dr0933 ) pathways [ 10 ]. The interrelation between trehalose, glycogen or maltose in vivo has been the most interesting subject in recent years [ 12 ]. The accumulation of metabolites,...”
- “...dr0463 ) Maltooligosyl trehalose synthase 5.2 treZ ( dr0464 ) Trehalose trehalohydrolase 3.5 treS ( dr0933 ) Trehalose synthase 5.7 dr1141 Pullulanase/amylase 3.9 ijms-24-03437-t002_Table 2 Table 2 Substrate specificity of DogH. Carbohydrate Monosaccharides and Bonds Present Cellobiose D-glucose--1,4-D-glucose a-cyclodextrin Cyclo-[-D-glucose--1,4-]6 Lactose D-galactose--1,4-D-glucose Maltose D-glucose--1,4-D-glucose Melibiose D-galactose--1,6-D-glucose...”
- Molecular and functional characterization of a unique sucrose hydrolase from Xanthomonas axonopodis pv. glycines
Kim, Journal of bacteriology 2004 - “...to SUH. These proteins, designated RB5196, CC1135, and DR0933, whose expression and functions have yet to be tested (although their functions were assigned as...”
- “...number AAK23119), a putative D. radiodurans -amylase (DR0933; accession number AAF10510), a putative Pirellula sp. -amylase or amylosucrase (RB5196; accession...”
- Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics
Makarova, Microbiology and molecular biology reviews : MMBR 2001 - “...probably uses an alternative pathway via trehalose synthase (DR0933), which has been recently characterized in Thermus (209). Trehalose plays a major role in...”
HTIA_0925 alpha-amylase family glycosyl hydrolase from Halorhabdus tiamatea SARL4B
25% identity, 27% coverage
Q1GY12 amylosucrase (EC 2.4.1.4) from Methylobacillus flagellatus (see 2 papers)
ABE50875.1 amylosucrase (MFAS;Mfla_2611) (EC 2.4.1.4) (see protein)
25% identity, 30% coverage
SCO3780 sugar hydrolase from Streptomyces coelicolor A3(2)
30% identity, 34% coverage
EF1349 glycosyl hydrolase, family 13 from Enterococcus faecalis V583
Q835M8 Alpha-amylase from Enterococcus faecalis (strain ATCC 700802 / V583)
EF_1349 alpha-glucosidase from Enterococcus faecalis V583
27% identity, 36% coverage
- Transcriptional response of Enterococcus faecalis V583 to erythromycin
Aakra, Antimicrobial agents and chemotherapy 2005 - “...EF2704 EF2705 EF2736 EF3113 EF0956 EF0957 EF1071 EF1347 EF1348 EF1349 EF1411 EF1543 EF1606 EF2646 EF0283 EF0848 EF1608 EF2691 EF2883 EF3112 EF2320 0 0 0 0 0 0...”
- “...EF0956 EF0957 EF1020 EF1068 EF1109 EF1211 EF1347 EF1348 EF1349 EF1526 EF1612 EF1613 EF1826 EF2589 EF2646 EF2863 EF2961 EF3157 EF0169 EF0514 EF0913 EF0302 EF0685...”
- Comparative proteomics analysis of biofilms and planktonic cells of Enterococcus faecalis and Staphylococcus lugdunensis with contrasting biofilm-forming ability
Cho, PloS one 2024 - “...EF_3207 tRNA-dihydrouridine synthase 19.22 21.65 0.89 Q832Z3 EF_0180 ABC transporter, permease protein 17.04 19.20 0.89 Q835M8 EF_1349 Alpha-amylase 16.73 18.92 0.88 Q835M9 EF_1348 Glucan 1,6-alpha-glucosidase, putative 17.35 19.66 0.88 S . lugdunensis A0A292DIE8 EQ812_06280 Cold shock protein CspA 25.22 22.34 1.13 A0A133Q308 EQ812_07575 Tautomerase 23.65 21.00...”
- Comparative proteomics analysis of biofilms and planktonic cells of Enterococcus faecalis and Staphylococcus lugdunensis with contrasting biofilm-forming ability
Cho, PloS one 2024 - “...tRNA-dihydrouridine synthase 19.22 21.65 0.89 Q832Z3 EF_0180 ABC transporter, permease protein 17.04 19.20 0.89 Q835M8 EF_1349 Alpha-amylase 16.73 18.92 0.88 Q835M9 EF_1348 Glucan 1,6-alpha-glucosidase, putative 17.35 19.66 0.88 S . lugdunensis A0A292DIE8 EQ812_06280 Cold shock protein CspA 25.22 22.34 1.13 A0A133Q308 EQ812_07575 Tautomerase 23.65 21.00 1.13...”
- “...permease protein, putative ( EF_2049 ), while glucan 1,6-alpha-glucosidase, putative ( EF_1348 ), Alpha-amylase ( EF_1349 ) and ABC transporter, permease protein ( EF_0180 ) were found to be more abundant in planktonic cell compared to in biofilm. Aside from the ABC transporter/permease proteins, the finding...”
OG1RF_11138 alpha-glucosidase from Enterococcus faecalis OG1RF
27% identity, 36% coverage
Rxyl_0315 Trehalose synthase-like protein from Rubrobacter xylanophilus DSM 9941
27% identity, 16% coverage
OJAG_12050 maltose alpha-D-glucosyltransferase from Oerskovia enterophila
23% identity, 75% coverage
Hsero_2326 maltose alpha-D-glucosyltransferase from Herbaspirillum seropedicae SmR1
28% identity, 15% coverage
Gmet_3469 Trehalose synthase/probable maltokinase-like from Geobacter metallireducens GS-15
27% identity, 17% coverage
PFREUD_10650 maltose alpha-D-glucosyltransferase from Propionibacterium freudenreichii subsp. shermanii CIRM-BIA1
27% identity, 35% coverage
BBMN68_1428, BL105A_1885 glycoside hydrolase family 13 protein from Bifidobacterium longum subsp. longum BBMN68
29% identity, 34% coverage
- Resistant starch utilization by Bifidobacterium, the beneficial human gut bacteria
Jung, Food science and biotechnology 2023 - “...encoding -amylase (BBMN68_650, BBMN68_1257), -glucosidase (BBMN68_1428 and BBMN68_1261), and -1, 6-glucosidase (BBMN68_1600 and BBMN68_1430) responsible for...”
- Application of Recombinase-Based In Vivo Expression Technology to Bifidobacterium longum subsp. longum for Identification of Genes Induced in the Gastrointestinal Tract of Mice
Koguchi, Microorganisms 2020 - “...1st L 67 BL105A_1857 Hypothetical protein 4th R, G 68 BL105A_1883 -Glucosidase 3rd G 69 BL105A_1885 Glycosidase 1st G 70 BL105A_1886 Permease protein of ABC transporter system for sugars 4th G 71 BL105A_1894 Raffinose transport system permease protein 2nd, 3rd G 72 BL105A_1910 Lipopolysaccharide kinase 3rd...”
- A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an IS3 Family Insertion Sequence, ISBlo11
Sakanaka, Applied and environmental microbiology 2018 - “...at least two other putative -glucosidases, BL105A_0107 and BL105A_1885, which have 96% and 99% amino acid identity to Bbr_0111 (Agl3) and Bbr_1857 (MelD),...”
- “...respectively, in B. breve UCC2003 (33). BL105A_1883 and BL105A_1885 are near each other, and a transposon insertion into BL105A_1883 was shown to reduce the...”
- Starch and starch hydrolysates are favorable carbon sources for bifidobacteria in the human gut
Liu, BMC microbiology 2015 - “...are responsible for degrading starch and starch hydrolysates. These genes encoded alpha-amylase (BBMN68_650, BBMN68_1257), alpha-glucosidase (BBMN68_1428, BBMN68_1261) and alpha-1, 6-glucosidase (BBMN68_1600, BBMN68_1430). It is speculated that these enzymes could completely degrade starch to glucose (Figure 3 ). Furthermore, BBMN68 contained genes encoding GH13 family sucrose phosphorylase...”
- “...BBMN68_1670 were 22-and 7-fold up-regulated, respectively (Figure 6 ). BBMN68_650 , BBMN68_1257 , BBMN68_1261 , BBMN68_1428 were also up-regulated when BBMN68 was grown on medium with maltodextrins with the sole carbon source. Our results showed that the predicted genes for degrading the starch and starch hydrolysates...”
Bbr_1857 glycoside hydrolase family 13 protein from Bifidobacterium breve UCC2003
29% identity, 34% coverage
- A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an IS3 Family Insertion Sequence, ISBlo11
Sakanaka, Applied and environmental microbiology 2018 - “...and 99% amino acid identity to Bbr_0111 (Agl3) and Bbr_1857 (MelD), respectively, in B. breve UCC2003 (33). BL105A_1883 and BL105A_1885 are near each other, and...”
- “...polar effects (Table S5). However, its homolog Bbr_1857 prefers trisaccharides rather than disaccharides (e.g., palatinose) and has lower hydrolytic activity...”
- Comparative genomics and genotype-phenotype associations in Bifidobacterium breve
Bottacini, Scientific reports 2018 - “...PF00128 OConnell et al . 23 Bbr_1856 Alpha-galactosidase X PF05691 OConnell et al . 23 Bbr_1857 Alpha-1,4-glucosidase PF00128 OConnell et al . 23 Bbr_1858 Permease protein of ABC transporter system for sugars PF00528 OConnell et al . 23 Bbr_1859 Permease protein of ABC transporter system for...”
- Transcription of two adjacent carbohydrate utilization gene clusters in Bifidobacterium breve UCC2003 is controlled by LacI- and repressor open reading frame kinase (ROK)-type regulators
O'Connell, Applied and environmental microbiology 2014 - “...Berkeley Bbr_1862 Bbr_1861 Bbr_1860 Bbr_1859 Bbr_1858 Bbr_1857 Bbr_1856 Regulation of Bifidobacterial Carbohydrate Metabolism growth on 1% melezitose or...”
- Metabolism of four α-glycosidic linkage-containing oligosaccharides by Bifidobacterium breve UCC2003
O'Connell, Applied and environmental microbiology 2013 - “...242 through 383 of the 620 codons of this gene), Bbr_1857 (designated melD) (456 bp, representing codons 92 to 183 of the 556 codons of this gene), Bbr_1860...”
- “...ID Bbr_0026 Bbr_0027 Bbr_0030 Bbr_0100 Bbr_1855 Bbr_1856 Bbr_1857 Bbr_1858 Bbr_1859 Bbr_1860 Bbr_1865 Bbr_1866 Bbr_1867 Bbr_1868 Bbr_1869 Gene name Function...”
A1XGB1 maltose alpha-D-glucosyltransferase from Propionibacterium freudenreichii subsp. shermanii
27% identity, 35% coverage
- Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1
Plaza-Vinuesa, Microbial cell factories 2019 - “...from GH13_4 ( Neisseria polysaccharea , accession Q9ZEU2), GH13_16 ( Propionibacterium freudenreichii subsp. Shermanii , A1XGB1), GH13_17 ( Apis mellifera , Q25BT8), GH13_18 ( Bifidobacterium adolescentis , Q84HQ2), GH13_20 ( Lactobacillus plantarum WCFS1, F9URM8), GH13_21 ( Escherichia coli , P21517), GH13_23 ( Xhantomonas campestris , Q76LB0),...”
4aieA / Q5FMB7 Structure of glucan-1,6-alpha-glucosidase from lactobacillus acidophilus ncfm (see paper)
25% identity, 35% coverage
- Ligand: calcium ion (4aieA)
Q5FMB7 glucan 1,6-alpha-glucosidase (EC 3.2.1.70) from Lactobacillus acidophilus (see paper)
AAV42157.1 oligo-α-1,6-glucosidase (LBA0264) (EC 3.2.1.10) (see protein)
LBA0264 glucan 1,6-alpha-glucosidase from Lactobacillus acidophilus NCFM
25% identity, 35% coverage
- In Vivo Transcriptome of Lactobacillus acidophilus and Colonization Impact on Murine Host Intestinal Gene Expression
Goh, mBio 2021 - “...primary chow ingredients. There was a preferential upregulation of catabolic machinery that targeted -1,4-/-1,6-glucoside (LBA1866-1867, LBA0264) and -glucoside (LBA1575-1576, LBA1706-1707) substrates in the ileum, reflecting the ability of L. acidophilus NCFM to harvest carbon source from starch components partially degraded by host enzymes at the upper...”
- Enzymology and structure of the GH13_31 glucan 1,6-α-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM
Møller, Journal of bacteriology 2012 - “...first 100 protein sequences from BLASTP searches using LBA0264 and LBA1872 were retrieved and complemented with lactobacillus sequences from CAZy (8) annotated...”
- “...carried out with the primer pairs targeting LBA0264 (encoding LaGH13_31) and genes in the maltooligosaccharide transport and catabolism gene cluster (LBA1866,...”
- Transcriptional analysis of prebiotic uptake and catabolism by Lactobacillus acidophilus NCFM
Andersen, PloS one 2012 - “...shown to be more active on isomaltooligosaccharides longer than isomaltose [42] . However, this locus (LBA0264, GH13_31) was not significantly upregulated in the current study. It is possible that this latter enzyme is induced on longer isomaltooligosaccharides, which may be transported via a different route. Such...”
- “...and polydextrose) and complementing the conventional route of degradation mediated by the putative -1,6 glucosidase (LBA0264) encoded in the genome of L. acidophilus NCFM. The important potential for GIT adaption of L. acidophilus NCFM by genetic loci encoding specific oligosaccharide utilization is further emphasized from genomic...”
ADD81256.1 α-amylase (Aamy1) (EC 3.2.1.20) (see protein)
28% identity, 30% coverage
A0A1I7MT66 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Micrococcus terreus (see paper)
25% identity, 45% coverage
PXO_03668 alpha-amylase family protein from Xanthomonas oryzae pv. oryzae PXO99A
28% identity, 18% coverage
5ykbD / I3NX86 The n253f mutant structure of trehalose synthase from deinococcus radiodurans reveals an open active-site conformation (see paper)
25% identity, 37% coverage
- Ligands: calcium ion; magnesium ion (5ykbD)
RradSPS_0192 maltose alpha-D-glucosyltransferase from Rubrobacter radiotolerans
26% identity, 16% coverage
GSU2361 alpha amylase family protein from Geobacter sulfurreducens PCA
26% identity, 17% coverage
BLGT_RS00840 glycoside hydrolase family 13 protein from Bifidobacterium longum subsp. longum GT15
26% identity, 31% coverage
SACE_6260 alpha-glucosidase from Saccharopolyspora erythraea NRRL 2338
26% identity, 34% coverage
BBMN68_1261 alpha-amylase family glycosyl hydrolase from Bifidobacterium longum subsp. longum BBMN68
26% identity, 31% coverage
- Resistant starch utilization by Bifidobacterium, the beneficial human gut bacteria
Jung, Food science and biotechnology 2023 - “...(BBMN68_650, BBMN68_1257), -glucosidase (BBMN68_1428 and BBMN68_1261), and -1, 6-glucosidase (BBMN68_1600 and BBMN68_1430) responsible for starch degradation,...”
- “...predicted starch pathway revealed that -glucosidase (BBMN68_1261), -1, 6-glucosidase (BBMN68_1600 and BBMN68_1430), -amylase (BBMN68_650), and two components of...”
- Starch and starch hydrolysates are favorable carbon sources for bifidobacteria in the human gut
Liu, BMC microbiology 2015 - “...responsible for degrading starch and starch hydrolysates. These genes encoded alpha-amylase (BBMN68_650, BBMN68_1257), alpha-glucosidase (BBMN68_1428, BBMN68_1261) and alpha-1, 6-glucosidase (BBMN68_1600, BBMN68_1430). It is speculated that these enzymes could completely degrade starch to glucose (Figure 3 ). Furthermore, BBMN68 contained genes encoding GH13 family sucrose phosphorylase (...”
- “...16 over-produced proteins, 4 were predicted to be involved in degrading starch and starch hydrolysates (BBMN68_1261, alpha-glucosidase; BBMN68_1430, alpha-glucosidase; BBMN68_1600, oligo-1,6-glucosidase; and BBMN68_650, alpha-amylase). In addition, 2 components of ABC-type sugar transporters (BBMN68_1403, BBMN68_1670) and a glyceraldehyde 3-phosphate dehydrogenase (BBMN68_254) showed more abundant. We then analyzed...”
BL0529 probable alpha-1,4-glucosidase; maltase-like enzyme from Bifidobacterium longum NCC2705
26% identity, 31% coverage
- The Pleiotropic Effects of Carbohydrate-Mediated Growth Rate Modifications in Bifidobacterium longum NCC 2705
Duboux, Microorganisms 2023 - “...1.834 0.000 4-alpha-glucanotransferase (amylomaltase) BL0528 malR2 0.349 0.664 Transcr. regulator of maltose/maltodextrin utilization, LacI family BL0529 aglA 0.925 0.063 Alpha-glucosidase BL0143 malF 0.713 0.222 Maltose/maltodextrin ABC transporter BL0144 malG 0.699 0.263 Maltose/maltodextrin ABC transporter...”
- Mixed-species genomic microarray analysis of fecal samples reveals differential transcriptional responses of bifidobacteria in breast- and formula-fed infants
Klaassens, Applied and environmental microbiology 2009 - “...Locus tag(s) BAD_0708 BAD_1412 BAD_1527 BAD_1605 BL0146 BL0421 BL0529 BL0536 BL0544 BL0597 BL0715 BL0716 BL0978 BL1104 BL1308 BL1518 BL1638 BL1639 BL1641 BL1643...”
- “...carbohydrate metabolism are predicted to encode an -1,4-glucosidase (BL0529) and an -galactosidase (BL1518), both of which are expressed to a greater degree in...”
- Differential transcriptional response of Bifidobacterium longum to human milk, formula milk, and galactooligosaccharide
González, Applied and environmental microbiology 2008 - “...BL0157 BL0671-BL0672 BL0433-BL0434 BL1545-BL1546 BL0976 BL0742 BL0284 BL0529 BL0544 BL0872 BL0966 BL0993-BL0994 BL1169-BL1170 BL1731-BL1732 MT FU 5.54 5.07 0...”
- “...milk. These included two putative glycosyl hydrolases (BL0529 and BL0544) (Tables 1 and 2) and an ATP-binding cassette (ABC)type transporter (BL1164-BL1165),...”
- Low-pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum
Sánchez, Applied and environmental microbiology 2007 - “...or 7a COGb Carbohydrate transport and metabolism WT ratiog rpe BL0529 BL1630 BL0988 BL0716 BL1644 BL0715 BL0978 BL1341 BL0753 50 60 55 25 34 65 39 30 36 3.49E...”
5jy7B / A0R6E0 Complex of mycobacterium smegmatis trehalose synthase with maltokinase (see paper)
30% identity, 33% coverage
- Ligand: calcium ion (5jy7B)
jpw_16785 maltose alpha-D-glucosyltransferase from Pseudomonas asiatica
28% identity, 17% coverage
- Genomic Analysis of Pseudomonas asiatica JP233: An Efficient Phosphate-Solubilizing Bacterium
Wang, Genes 2022 - “...OtsA/OtsB, TreS, TreY/TreZ, TreP, and TreT [ 70 ]. In the present study, the treS (jpw_16785), treY (jpw_16755) and treZ (jpw_16745) genes of the TreS and TreY/TreZ pathways were detected in the genome of strain JP233. More than 30 genes were identified in the P. putida...”
- “...pvdS extracytoplasmic-function sigma-70 factor jpw_17575 fpvI sigma-70 family RNA polymerase sigma factor jpw_17710 acetyltransferase Trehalose jpw_16785 treS trehalose synthase jpw_16755 treY malto-oligosyltrehalose synthase jpw_16745 treZ malto-oligosyltrehalose trehalohydrolase Acetoin and 2,3-butanediol jpw_12445 ilvB acetolactate synthase large subunit jpw_19870 ilvB acetolactate synthase large subunit jpw_22100 ilvH acetolactate synthase...”
TRES_MYCTU / P9WQ19 Trehalose synthase/amylase TreS; Maltose alpha-D-glucosyltransferase; MTase; EC 3.2.1.1; EC 5.4.99.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
P9WQ19 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Mycobacterium tuberculosis (see paper)
NP_214640 trehalose synthase/amylase TreS from Mycobacterium tuberculosis H37Rv
MT0134 alpha-amylase family protein from Mycobacterium tuberculosis CDC1551
Rv0126 TREHALOSE SYNTHASE TRES from Mycobacterium tuberculosis H37Rv
RVBD_0126 maltose alpha-D-glucosyltransferase from Mycobacterium tuberculosis H37Rv
27% identity, 34% coverage
- function: Catalyzes the reversible interconversion of maltose and trehalose by transglucosylation (PubMed:18505459). Also displays amylase activity, catalyzing the endohydrolysis of (1->4)-alpha-D- glucosidic linkages in glycogen and maltooligosaccharides such as maltoheptaose, to produce maltose which then can be converted to trehalose (PubMed:18505459). TreS plays a key role in the utilization of trehalose for the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P) (PubMed:18505459, PubMed:27513637). Might also function as a sensor and/or regulator of trehalose levels within the cell (PubMed:18505459). Thus, when trehalose levels in the cell become dangerously low, TreS could expedite the conversion of glycogen to maltose via its amylase activity and then convert the maltose to trehalose; but this enzyme also could expedite or promote the conversion of trehalose to glycogen when cytoplasmic trehalose levels become too high (PubMed:18505459).
catalytic activity: D-maltose = alpha,alpha-trehalose (RHEA:15145)
catalytic activity: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D- glucose units.
subunit: Homohexamer.
disruption phenotype: Cells lacking this gene are as virulent as wild- type in mice, however also show a profound impact on intracellular and capsular glucan (PubMed:20305657, PubMed:27513637). It is not possible to inactivate Rv3032 in a mutant lacking treS, suggesting the joint essentiality of the different alpha-(1->4)-glucans biosynthesis pathways involving these two genes (PubMed:20305657). Combined inactivation of glgM and treS results in absence of alpha-glucan (PubMed:27513637). Combined inactivation of treS and glgC results in absence of alpha-glucan content and is significantly attenuated for growth in the lung and spleen of BALB/C mice during both the acute and chronic phase of infection (PubMed:27513637). - Assessing the propensity of TB clinical isolates to form viable but non-replicating subpopulations
Coetzee, Scientific reports 2024 - “...acids, is essential for M. tuberculosis persistence in mice 60 , 61 . Rv0126 S112dx P9WQ19 treS E252Q 601 Trehalose synthase/amylase (MTase) M. tuberculosis requires TreS for virulence in a mouse model and drug-tolerance 62 , 63 . Rv1194c S169dx O05296 rv1194c F114L 421 Conserved protein...”
- The unfoldase ClpC1 of Mycobacterium tuberculosis regulates the expression of a distinct subset of proteins having intrinsically disordered termini
Lunge, The Journal of biological chemistry 2020 (secret) - Systematic Analysis of Metabolic Pathway Distributions of Bacterial Energy Reserves
Wang, G3 (Bethesda, Md.) 2019 - “...branching enzyme (GH13) 728 P07762 # PF02922, PF00128, PF02806 Mycobacterium tuberculosis treS Trehalose synthase/amylase 601 P9WQ19 ! PF00128, PF16657 Mycobacterium tuberculosis pep2 Maltokinase 455 Q7DAF6 ! PF18085 Mycobacterium tuberculosis glgE Alpha-1,4-glucan: maltose-1-phosphate Maltosyltransferase 701 P9WQ17 ! PF00128, PF11896 Mycobacterium tuberculosis Rv3032 Glycogen synthase 414 P9WMY9 PF13439,...”
- Bioinformatics Analysis of Metabolism Pathways of Archaeal Energy Reserves
Wang, Scientific reports 2019 - “...debranching enzyme 657 P15067 PF02922 PF00128 PF02806 Mycobacterium tuberculosis B treS TreS Trehalose synthase/amylase 601 P9WQ19 PF00128 PF16657 Mycobacterium tuberculosis B pep2 Pep2 Maltokinase 455 Q7DAF6 / Picrophilus torridus A glgE GlgE Alpha-1,4-glucan: maltose-1-phosphate maltosyltransferase 630 Q6L2Z8 PF11896 PF00128 Mycobacterium tuberculosis B Rv3032 Rv3032 Glycogen synthase...”
- Comparison of the terrestrial cyanobacterium Leptolyngbya sp. NIES-2104 and the freshwater Leptolyngbya boryana PCC 6306 genomes
Shimura, DNA research : an international journal for rapid publication of reports on genes and genomes 2015 - “...long C-terminal extension compared with TreS of Mycobacterium tuberculosis H37Rv (NCBI reference sequence accession number: NP_214640). Enzymatic characterization of the treS gene product will be needed to better understand trehalose-related metabolism in these cyanobacteria. Table 2. Trehalose phosphorylase enzymes in two Leptolyngbya strains Length, amino acid...”
- Transcriptional Profiling of Mycobacterium tuberculosis Exposed to In Vitro Lysosomal Stress
Lin, Infection and immunity 2016 - “...0.8 0.1 1.1 1.3 0.1 1.9 1.1 0.4 0.6 MT0134 MT0254 MT0265 MT0289 MT0365 MT0366 MT0367 MT0397 Rv0126 Rv0240 Rv0251c Rv0277c Rv0350 Rv0351 Rv0352 Rv0384c treS...”
- Targeting Mycobacterium tuberculosis Persistence through Inhibition of the Trehalose Catalytic Shift
Kalera, ACS infectious diseases 2024 - “...d -trehalose analogues block the Mtb trehalose catalytic shift through inhibition of trehalose synthase TreS (Rv0126), which catalyzes the isomerization of trehalose to maltose. Out of a focused eight-member compound panel constructed by chemoenzymatic synthesis, the natural product 2-trehalosamine exhibited the highest potency and significantly potentiated...”
- “...10 , 14 17 The trehalose catalytic shift depends on the Mtb trehalose synthase TreS (Rv0126), 18 which catalyzes the isomerization of trehalose to maltose, thus serving as a link between trehalose metabolism and CCM ( Figure 1 B). Although TreS is not strictly essential for...”
- Assessing the propensity of TB clinical isolates to form viable but non-replicating subpopulations
Coetzee, Scientific reports 2024 - “...of fatty acids, is essential for M. tuberculosis persistence in mice 60 , 61 . Rv0126 S112dx P9WQ19 treS E252Q 601 Trehalose synthase/amylase (MTase) M. tuberculosis requires TreS for virulence in a mouse model and drug-tolerance 62 , 63 . Rv1194c S169dx O05296 rv1194c F114L 421...”
- Genome-wide screen identifies host loci that modulate Mycobacterium tuberculosis fitness in immunodivergent mice
Meade, G3 (Bethesda, Md.) 2023 - “...24.850 3.37 18.284 35.583 1.22E02 hp089 Rv3841 bfrB 11 47.049 3.28 35.903 80.586 1.67E02 hp090 Rv0126 treS 11 65.541 3.56 64.895 81.165 3.85E02 hp091 Rv2116 lppK 11 73.243 4.46 73.194 81.165 1.67E02 hp092 Rv0765c Rv0765c 11 106.661 4.70 106.040 107.457 2.58E02 hp093 Rv1850 ureC 11 107.742...”
- Genomic analysis of Mycobacterium brumae sustains its nonpathogenic and immunogenic phenotype
Renau-Mínguez, Frontiers in microbiology 2022 - “...brumae . Locus Gene name Start End Strand Function Protein identity (%) Query coverage (%) Rv0126 treS 152,324 154,129 Forward Lipid Synthesis 82.7 94.7 Rv0129c fbpC 156,578 157,600 Reverse Secreted antigen 71 96.2 Rv0198c zmp1 234,516 236,507 Reverse Metallo-protease 75.6 100 Rv0148 Rv0148 174,833 175,693 Forward...”
- “...PIM, TDM, TMM, and other PL production, we detected 13 genes in M . brumae (Rv0126, Rv0129c, Rv0982, Rv1166, Rv1236-Rv1238, Rv1564c, Rv2188, Rv2610c, Rv2869c, Rv3264c, and Rv3793). Intraspecific variability To assess the intraspecific variation among the different M . brumae strains, we analyzed the genomes of...”
- Insights into the molecular determinants involved in Mycobacterium tuberculosis persistence and their therapeutic implications
Joshi, Virulence 2021 - “...cytochrome biogenesis Low oxygen, nitric oxide [ 88 , 89 ] Virulence, detoxification, and adaptation Rv0126 treS Trehalose synthase Biosynthesis of trehalose - [ 45 ] Rv2031c hspX Heat shock protein Proposed role in the maintenance of long-term viability or replication during latent or initial infections,...”
- “...in isoniazid treated chronically infected mice [ 88 , 89 ] Virulence, detoxification and adaptation Rv0126 ( treS ) Deletion mutant shows increased rate of mice survival [ 45 ] Rv2031c ( hspX ) Deletion mutant shows increased bacterial growth upon tuberculosis infection in mice as...”
- An Overview of Genetic Information of Latent Mycobacterium tuberculosis
Hamidieh, Tuberculosis and respiratory diseases 2021 - “...Genes involved in cell wall changes Rv0470c pcaA Cyclopropane mycolic acid synthase 3 864 560,848 Rv0126 treS Trehalose synthase 1,806 152,354 Signal transduction Rv3133c dosR Two-comp. resp. reg 654 3,499,262 Rv3132c dosS Two-comp. resp. reg 1,737 3,497,529 Rv2027c dosT Two-comp. resp. reg 1,722 2,272,787 Rv0757 phoP...”
- SEVERITY OF TB CLASSIFIED BY MODIFIED BANDIM TB SCORING ASSOCIATES WITH THE SPECIFIC SEQUENCE OF ESXA GENES IN MDR-TB PATIENTS
Dewi, African journal of infectious diseases 2020 - “...et al., 2016). Jhingan et al. also added virulence-related proteins, such as Rv2780 (L-alanine dehydrogenase), Rv0126 (TreS), Rv2299 (HtpG), Rv903c (PrrA), Rv3133c (Devr) and Rv0042c, were secreted at low levels in M. tuberculosis JAL (Jhingan et al., 2016). These studies reinforced the assertion that the esxA...”
- More
- Host-pathogen genetic interactions underlie tuberculosis susceptibility in genetically diverse mice
Smith, eLife 2022 - “...77.63 81.03 Hip24 mce4A RVBD_3499 c mod16 7.91 4.12E-02 10 78.88 81.36 88.25 Hip25 treS RVBD_0126 mod7 7.94 3.04E-02 11 20.80 36.14 44.06 Hip26 pckA RVBD_0211 mod3 7.67 4.74E-02 11 85.95 89.78 91.75 Hip27 aspB RVBD_3565 mod7 8.32 3.66E-02 11 114.69 116.99 117.08 Hip28 rv1227c RVBD_1227...”
treS / A0R6E0 trehalose synthase subunit (EC 5.4.99.16) from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (see 4 papers)
TRES_MYCS2 / A0R6E0 Trehalose synthase/amylase TreS; Maltose alpha-D-glucosyltransferase; MTase; EC 3.2.1.1; EC 5.4.99.16 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 6 papers)
A0R6E0 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Mycolicibacterium smegmatis (see 3 papers)
MSMEG_6515 trehalose synthase from Mycobacterium smegmatis str. MC2 155
30% identity, 32% coverage
- function: Catalyzes the reversible interconversion of maltose and trehalose by transglucosylation (PubMed:15511231, PubMed:18505459, PubMed:20118231, PubMed:21840994). Maltose is the preferred substrate (PubMed:15511231, PubMed:18505459). To a lesser extent, also displays amylase activity, catalyzing the endohydrolysis of (1->4)-alpha-D- glucosidic linkages in glycogen and maltooligosaccharides such as maltoheptaose, to produce maltose which then can be converted to trehalose (PubMed:18505459). TreS plays a key role in the utilization of trehalose for the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P) (PubMed:20118231, PubMed:27513637). Might also function as a sensor and/or regulator of trehalose levels within the cell. Thus, when trehalose levels in the cell become dangerously low, TreS can expedite the conversion of glycogen to maltose via its amylase activity and then convert the maltose to trehalose; but this enzyme also can expedite or promote the conversion of trehalose to glycogen when cytoplasmic trehalose levels become too high. Is also able to catalyze the hydrolytic cleavage of alpha-aryl glucosides, as well as alpha-glucosyl fluoride in vitro.
catalytic activity: D-maltose = alpha,alpha-trehalose (RHEA:15145)
catalytic activity: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D- glucose units.
subunit: Homohexamer.
disruption phenotype: Cells lacking this gene do not accumulate increased amounts of glycogen in the presence of trehalose and show only a small effect in alpha-glucan (PubMed:20118231, PubMed:27513637). Combined inactivation of treS with glgB or glgC completely blocks alpha-glucan production (PubMed:27513637). - Crystal structure of the TreS:Pep2 complex, initiating α-glucan synthesis in the GlgE pathway of mycobacteria
Kermani, The Journal of biological chemistry 2019 - “...in mycobacteria. Experimental procedures Cloning of M. smegmatis Pep2, TreS The DNA sequences of TreS (MSMEG_6515) and Pep2 (MSMEG_6514) were cloned from M. smegmatis genomic DNA by polymerase chain reaction (PCR) using the following primers: TreS forward, GATCGATC CATATG GAGGAGCACACGCAGGGCAG, and TreS reverse, GATCGATC AAGC TT...”
- Protein Composition of Mycobacterium smegmatis Differs Significantly Between Active Cells and Dormant Cells With Ovoid Morphology
Trutneva, Frontiers in microbiology 2018 - “...found several enzymes responsible for the synthesis and accumulation of glycogen (MSMEG_4918), trehalose (MSMEG_6514 and MSMEG_6515 unique; Supplementary Table S2 ) and polyphosphates (MSMEG_2391). All these enzymes were found in the dormant cell proteome only (unique proteins). Degradation Processes The proteome of dormant Msm is enriched...”
- Characterization of Mycobacterium smegmatis sigF mutant and its regulon: overexpression of SigF antagonist (MSMEG_1803) in M. smegmatis mimics sigF mutant phenotype, loss of pigmentation, and sensitivity to oxidative stress
Singh, MicrobiologyOpen 2015 - “...Hypothetical protein 3.17/2.95 GTCGN 17 GGGCC 1008 MSMEG_6514 Trehalose synthasefused maltokinase 1.98/2.75 GTGTN 16 GGGTA MSMEG_6515 Trehalose synthase 2.03/2.58 GTGTN 16 GGGTA 10 MSMEG_6606 Hypothetical protein 3.15/2.45 GTTCN 14 GGGCA MSMEG_6607 Hypothetical protein 3.51/2.52 GTTCN 14 GGGCA MSMEG_6608 Hypothetical protein 4.87/2.97 GTTCN 14 GGGCA MSMEG_6609 Hypothetical...”
- “...profile of nonpolar lipids in mutant strain. Concomitant with these findings trehalose biosynthesis genes (MSMEG_6514, MSMEG_6515) and mycocerosic acid synthase genes (MSMEG_6765 to MSMEG_6767) were found to be significantly downregulated in sigF strain (Table 1 ). MSMEG_6515 encodes for trehalose synthase which enables the conversion of...”
- The SigF regulon in Mycobacterium smegmatis reveals roles in adaptation to stationary phase, heat, and oxidative stress
Hümpel, Journal of bacteriology 2010 - “...dps1 ACAACGATCTGCATCTGACG (forward) GTCACGCTCGACGGAGTAGT (reverse) MSMEG_6515 treS GGCGACTTCTACGTCTGGAG (forward) CGGGTTGTCGTAGTTGAGGT (reverse) strains in both...”
- “...MSMEG_6213 (mcat), MSMEG_6232 (katA), MSMEG_6467 (dps1), and MSMEG_6515 (treS) were determined by both microarray analysis (solid bars) and quantitative...”
- A genomic view of sugar transport in Mycobacterium smegmatis and Mycobacterium tuberculosis
Titgemeyer, Journal of bacteriology 2007 - “...homologs (msmeg_3184, msmeg_3576, msmeg_4916, msmeg_4917, msmeg_4696, and msmeg_6515) of MalL of B. subtilis, which hydrolyzes maltose, longer maltodextrines up...”
4lxfB Crystal structure of m. Tuberculosis tres (see paper)
27% identity, 37% coverage
- Ligand: calcium ion (4lxfB)
ACSP50_5263 maltose alpha-D-glucosyltransferase from Actinoplanes sp. SE50/110
28% identity, 32% coverage
A8QX00 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Actinoplanes sp. (see paper)
28% identity, 32% coverage
8uzhB / P9WQ19,Q12306 Sumo fused trehalose synthase (tres) of mycobacterium tuberculosis (see paper)
27% identity, 32% coverage
- Ligand: calcium ion (8uzhB)
Q88FN0 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Pseudomonas putida (see 2 papers)
PP_4059 trehalose synthase, putative from Pseudomonas putida KT2440
28% identity, 17% coverage
PPUTLS46_012155 maltose alpha-D-glucosyltransferase from Pseudomonas putida LS46
28% identity, 17% coverage
JNO42_02760 maltose alpha-D-glucosyltransferase from Pseudomonas putida
28% identity, 17% coverage
AAA57313.1 α-glucosidase (AglA) (EC 3.2.1.20) (see protein)
AAZ54871.1 α-glucosidase (Tfu_0833) (EC 3.2.1.20) (see protein)
Q60027 Alpha-1,4-glucosidase from Thermomonospora curvata
29% identity, 29% coverage
- Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1.
Plaza-Vinuesa, Microbial cell factories 2019 - “...Xhantomonas campestris , Q76LB0), GH_29 ( Bacillus subtilis , P39795), GH13_30 ( Thermonospora curvata , Q60027), GH13_31 ( Bacillus cereus , P21332), GH13_34 ( Xenopus leavis , Q7ZYR3), GH13_35 ( Xenopus leavis , Q32NL8), GH13_36 ( Paenibacillus polymyxa E681, E0RLH8) subfamilies and Lp_0179 (NI, not included...”
LOC107993621 maltase 1 from Apis cerana
24% identity, 49% coverage
Q6L2Z7 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Picrophilus torridus (see 2 papers)
28% identity, 30% coverage
XAC0155 trehalose synthase from Xanthomonas axonopodis pv. citri str. 306
28% identity, 18% coverage
- The dual nature of trehalose in citrus canker disease: a virulence factor for Xanthomonas citri subsp. citri and a trigger for plant defence responses
Piazza, Journal of experimental botany 2015 - “...to trehalose by trehalose synthase (EC 5.4.99.16), which is encoded by the treS gene (locus XAC0155) ( Nishimoto et al. , 1996 ). Fig. 1. Analysis of the expression levels of otsA , ostB , glgY , and TS in XccWT by RT-qPCR assays. (A) Representation...”
- “...1996 ). In Xcc, these two activities reside in the same bifunctional enzyme encoded by XAC0155, with an N-terminal treS domain and C-terminal maltokinase domain. The resulting -M1P is the substrate for synthesis of linear -1,4-glucan chains by glucan synthase (glgE; XAC0154), which are then branched...”
- Structure determination of a sugar-binding protein from the phytopathogenic bacterium Xanthomonas citri
Medrano, Acta crystallographica. Section F, Structural biology communications 2014 - “...(XAC0427), maltooligosyltrehalose synthase (XAC0429), trehalose synthase (XAC0155) and trehalose 6P synthase (XAC3211) has been identified in the X....”
XP_001660907 maltase 2 from Aedes aegypti
29% identity, 29% coverage
P72235 Trehalose synthase from Pimelobacter sp. (strain R48)
29% identity, 32% coverage
FPSE_01774 hypothetical protein from Fusarium pseudograminearum CS3096
24% identity, 39% coverage
- Response of Fusarium pseudograminearum to Biocontrol Agent Bacillus velezensis YB-185 by Phenotypic and Transcriptome Analysis
Zhang, Journal of fungi (Basel, Switzerland) 2022 - “...( FPSE_10579 , FPSE_05068 , FPSE_07610 , FPSE_08639 , FPSE_08884 , FPSE_08802 ), amylase ( FPSE_01774 , FPSE_06584 , FPSE_05690 ), endo-beta-1,4-glucanase ( FPSE_01902 ), endoglucanase ( FPSE_00619) , endo-1,4-beta-xylanase ( FPSE_05752 , FPSE_07423 ), and laccase ( FPSE_07047 , FPSE_08810 ) were all significantly down-regulated...”
- “...1.51 * gene4905 FPSE_08884 lipase_3 0.26 1.21 * gene9418 FPSE_08802 lipase_GDSL_2 1.02 4.36 * gene1287 FPSE_01774 alpha-amylase 0.88 1.25 * gene6132 FPSE_06584 alpha-amylase 0.45 6.94 * gene3826 FPSE_05690 alpha-amylase 1.37 * 3.29 * gene5852 FPSE_01902 endo-beta-1,4-glucanase D 0.54 5.95 * gene2343 FPSE_00619 endoglucanase-4 0.38 2.26 *...”
M1PA89 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Rhodococcus opacus (see paper)
29% identity, 30% coverage
A0A4S2BJW1 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Pseudomonas stutzeri (see paper)
26% identity, 16% coverage
BIF_00496 glycoside hydrolase family 13 protein from Bifidobacterium animalis subsp. lactis BB-12
26% identity, 32% coverage
- Updated Genome Sequence for the Probiotic Bacterium Bifidobacterium animalis subsp. lactis BB-12
Jensen, Microbiology resource announcements 2021 - “...544491 20bp35bp Coding(1,6051,624/1,653nt) BIF_01621 551925 3bp17bp Coding(3133/1,518nt) BIF_02058 551931 1bp Coding(37/1,518nt) BIF_02058 555527 62bp77bp Intergenic(+44/+177) BIF_00496 / BIF_01682 574023 + AACCCGCCC Intergenic(880/+30) BIF_16SrRNA12 / BIF_01549 601285 1bp Coding(2/771nt) BIF_00943 607329 +AC Coding(148/159nt) BIF_02197 607331 GC A50P(GC G CC C ) BIF_02197 627558 1bp Intergenic(+54/170) BIF_01669 /...”
A1IHL0 alpha-glucosidase (EC 3.2.1.20) from Apis cerana japonica (see paper)
BAF44218.1 α-glucosidase I (JBgI) (EC 3.2.1.20) (see protein)
jbgI / BAF44218.1 alpha-glucosidase isozyme I from Apis cerana japonica (see paper)
26% identity, 40% coverage
- Characterization of Glycoside Hydrolase Families 13 and 31 Reveals Expansion and Diversification of α-Amylase Genes in the Phlebotomine Lutzomyia longipalpis and Modulation of Sandfly Glycosidase Activities by Leishmania Infection
da, Frontiers in physiology 2021 - “...oryzae (Uniprot P0C1B3) ( Toda et al., 1982 ), maltase from Apis cerana japonica (Uniprot A1IHL0) ( Wongchawalit et al., 2006 ), amino acid transport protein (UniProt Q07837) ( Bertran et al., 1993 ) from Homo sapiens , glucan branching enzyme from E. coli (UniProt P07762)...”
- Essential (Mg, Fe, Zn and Cu) and Non-Essential (Cd and Pb) Elements in Predatory Insects (Vespa crabro and Vespa velutina): A Molecular Perspective
Andreani, International journal of molecular sciences 2020 - “...15 12 Apis mellifera A0A088A777 Sodium channel protein 292,184 17 2 2 Apis mellifera 2 A1IHL0 Alpha-glucosidase isozyme 66,623 52 2 1 Apis cerana japonica A0A088AUT8 Trehalase 77,540 37 2 2 Apis mellifera BIP_DROME Endoplasmic reticulum chaperone BiP 72,330 59 3 3 Drosophila melanogaster 3 A0A088AHC8...”
O06458 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Thermus thermophilus (see 3 papers)
27% identity, 18% coverage
TM1840 alpha-amylase from Thermotoga maritima MSB8
26% identity, 33% coverage
- Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2
Xu, BioMed research international 2015 - “...heterologous overexpression. Coding regions of Csac_1076 and Csac_1078 were fused to the signal peptide of TM1840 ( amyA ) and TM0070 ( xynB ), resulting in three chimeric enzymes, namely, TM1840-Csac_1078, TM0070-Csac_1078, and TM0070-Csac_1076, which were carried by Thermotoga-E. coli shuttle vectors pHX02, pHX04, and pHX07,...”
- “...of foreign proteins in T. sp. strain RQ2. A literature search revealed that T. maritima TM1840 (amylase A, AmyA) [ 19 21 ] and TM0070 (xylanase B, XynB) [ 20 ] have been experimentally confirmed to be secretive proteins. The former is anchored on the toga...”
- Genes for the major structural components of Thermotogales species' togas revealed by proteomic and evolutionary analyses of OmpA and OmpB homologs
Petrus, PloS one 2012 - “...toga [5] . Of the hydrolytic enzymes isolated from T. maritima , an amylase (AmyA, TM1840) and a xylanase (XynA, TM0061) have been definitively associated with the toga [4] , [5] . Only two other proteins, OmpA1 (previously called Omp) [6] , [7] and OmpB (previously...”
- “...OmpF in response to different environmental signals. To date, only three toga proteins (the amylase TM1840, xylanase TM0061 and OmpA1 TM0477) have been successfully annotated in the T. maritima genome sequence through cloning and sequencing of their genes [4] [6] , [12] , [13] . Using...”
- The genus Thermotoga: recent developments
Frock, Environmental technology 2010 - “...added to these processes, as it is a requisite cofactor for -amylase function. The -amylase (TM1840) from T. maritima has been expressed in tobacco cell cultures with a significant increase in thermostability compared to the E. coli -produced version, due to the intrinsic calcium levels in...”
- Evolution of mal ABC transporter operons in the Thermococcales and Thermotogales
Noll, BMC evolutionary biology 2008 - “...TM1834 TM1834 TM0690 TM1836 TM1835 TM1835 TM1837 TM1836 TM1836 TM1838 TM1203 TM1203 TM1839 TM1839 TM1839 TM1840 TM1840 TM1840 TM1841 TM1841(split?) TM1841 TM1842 X TM1842 TM1843 X TM1843 TM1844 TM1844 TM1844 TM1845 TM1845 TM1845 Bold, transporter ORFs; Italics, has at least one homolog in this region in...”
- Responses of wild-type and resistant strains of the hyperthermophilic bacterium Thermotoga maritima to chloramphenicol challenge
Montero, Applied and environmental microbiology 2007 - “...TM0545-0555a NC 2.52 2.82 NC Sugar utilization TM1068 TM1195 TM1219-1223a TM1835 TM1840 TM1845 NC NC NC NC NC NC 2.1 2.1 2.22 2.0 2.8 2.2 1.9 2.0 2.02 2.9 3.3...”
- “...Val, Leu, Ile biosynthesis Sugar utilization TM1068 TM1195 TM1219-1223a TM1835 TM1840 TM1845 NC NC NC NC NC NC 1.6 1.72 3.72 4.22 NC NC NC NC 3.62 3.92 2.22...”
- Several archaeal homologs of putative oligopeptide-binding proteins encoded by Thermotoga maritima bind sugars
Nanavati, Applied and environmental microbiology 2006 - “...1.5 9.5 1 TM1834 TM1835 TM1840 TM1845 -Glucosidase (cytoplasmic) Cyclomaltodextrinase (cytoplasmic) -Amylase (extracellular) Pullulanase (extracellular) TM1851...”
- The Thermotoga maritima phenotype is impacted by syntrophic interaction with Methanococcus jannaschii in hyperthermophilic coculture
Johnson, Applied and environmental microbiology 2006 - “...TM1746-49 TM1751 TM1752 TM1834 TM1835 TM1836 TM1839 TM1840 TM1848 TM1851 TM1853-55 Annotation Laminarinase -Glucan ABC transporter subunits Xylanase Xylan ABC...”
- Substrate specificities and expression patterns reflect the evolutionary divergence of maltose ABC transporters in Thermotoga maritima
Nanavati, Journal of bacteriology 2005 - “...TM1839. The starch group included TM0364, TM1069, TM1835, TM1839, TM1840, and TM1845. The genes in the guar gum group were TM1192, TM1204, TM1227, and TM1624....”
- “...419 TM1069 Transcriptional regulator; DeoR family 26c TM1839 TM1840 Maltose ABC transporter; PBP; MalE2 -Amylase, AmyA 63c 238 and 54c TM1069 Transcriptional...”
- More
XP_001689202 alpha-glucosidase from Anopheles gambiae
26% identity, 30% coverage
CAA72194.1 α-amylase (AmyA;TM1840;Tmari_1848) (Amy13A) (EC 3.2.1.1) (see protein)
amyA / CAA72194.1 alpha-amylase from Thermotoga maritima (see paper)
P96107 Alpha-amylase from Thermotoga maritima
26% identity, 33% coverage
F9URM8 Maltogenic alpha-amylase from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
lp_2757 glucan 1,4-alpha-maltohydrolase from Lactobacillus plantarum WCFS1
29% identity, 17% coverage
- Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1.
Plaza-Vinuesa, Microbial cell factories 2019 - “...lowercase letters). The gene lp_2757 (1,722bp) encoding a protein annotated as maltogenic -amylase (UniProtKB code: F9URM8) was amplified by using primers 1800 (5- TAACTTTAAGAAGGAGATATACAT atgcaactagctggaattaggcacc) and 1801 ( GCTATTAATGATGATGATGATGATG agccacggttaattcaaagcctcc). Purified PCR products were inserted into the pURI3-Cter vector using a restriction enzyme- and ligation-free cloning...”
- “...Apis mellifera , Q25BT8), GH13_18 ( Bifidobacterium adolescentis , Q84HQ2), GH13_20 ( Lactobacillus plantarum WCFS1, F9URM8), GH13_21 ( Escherichia coli , P21517), GH13_23 ( Xhantomonas campestris , Q76LB0), GH_29 ( Bacillus subtilis , P39795), GH13_30 ( Thermonospora curvata , Q60027), GH13_31 ( Bacillus cereus , P21332),...”
- Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1
Plaza-Vinuesa, Microbial cell factories 2019 - “...included in GH13 family. Among these, proteins annotated as maltose-forming -amylase (Lp_0179) and maltogenic -amylase (Lp_2757) were included. Results In this study, Lp_0179 and Lp_2757 L. plantarum -amylases were structurally and biochemically characterized. Lp_2757 displayed structural features typical of GH13_20 subfamily which were absent in Lp_0179....”
- “...carbohydrates, Lp_0179 only hydrolysed maltopentaose and dextrin, demonstrating that is an exotype glucan hydrolase. However, Lp_2757 was also able to hydrolyze cyclodextrins and other non-cyclic oligo- and polysaccharides, revealing a great preference towards -1,4-linkages typical of maltogenic amylases. Conclusions The substrate range as well as the...”
- Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays
Saulnier, Applied and environmental microbiology 2007 - “...lp_2096 lp_2097 lp_2151 lp_2152 lp_2153 lp_2154 lp_2371 lp_2757 lp_2684 lp_2776 lp_2794 lp_2920 lp_2969 lp_3010 lp_3011 lp_3045 lp_3092 lp_3170 1p_3279 lp_3284...”
HZ99_RS03675 maltose alpha-D-glucosyltransferase from Pseudomonas fluorescens
27% identity, 16% coverage
- Combined Transcriptome and Proteome Analysis of RpoS Regulon Reveals Its Role in Spoilage Potential of Pseudomonas fluorescens
Liu, Frontiers in microbiology 2019 - “...GlgX 0.299 (2.46E-05) 0.747 (4.89E-02) Down/Down HZ99_RS03670 WP_038441402.1 1,4-alpha-glucan branching protein GlgB 0.210 (1.57E-07) Down/ HZ99_RS03675 WP_038441404.1 Maltose alpha-D-glucosyltransferase TreS 0.298 (1.60E-05) Down/ HZ99_RS03680 WP_038441406.1 Alpha-1,4-glucanmaltose-1-phosphate maltosyltransferase GlgE 0.247 (6.95E-07) HZ99_RS10715 WP_038442912.1 Phosphoethanolamine transferase OpgE 0.237 (2.95E-02) 0.553 (1.08E-02) Down/Down HZ99_RS19405 WP_038445324.1 Maltodextrin phosphorylase GlgP 0.190...”
CPA40_RS02640 glycoside hydrolase family 13 protein from Bifidobacterium callitrichos
27% identity, 33% coverage
- The comparative genomics of Bifidobacterium callitrichos reflects dietary carbohydrate utilization within the common marmoset gut
Albert, Microbial genomics 2018 - “...Sucrose phosphorylase 2.4.1.7 COO72_RS10000 CPA40_RS07310 BCAL_RS06670 UTPglucose-1-phosphate uridylyltransferase 2.7.7.9 COO72_RS10475 CPA40_RS09980 BCAL_RS08755 -Amylase 3.2.1.1 COO72_RS11340 CPA40_RS02640 BCAL_RS02400 -Glucosidase 3.2.1.21 COO72_RS09740 CPA40_RS01270 BCAL_RS01445 Isoamylase 3.2.1.68 COO72_RS04240 CPA40_RS05215 BCAL_RS04710 Glucose-1-phosphate adenylyltransferase 2.7.7.27 COO72_RS01125 CPA40_RS05455 BCAL_RS09705 1,4--Glucan branching enzyme 2.4.1.18 COO72_RS03710 CPA40_RS0112 BCAL_RS04065 4--Glucanotransferase 2.4.1.25 COO72_RS08655 CPA40_RS05200 BCAL_RS04695 Glycogen...”
8ibkA / A0A2Z5WH92 Crystal structure of bacillus sp. Ahu2216 gh13_31 alpha-glucosidase e256q/n258g in complex with maltotriose (see paper)
27% identity, 36% coverage
- Ligands: alpha-d-glucopyranose; calcium ion (8ibkA)
MOC_1982 maltose alpha-D-glucosyltransferase from Methylobacterium oryzae CBMB20
23% identity, 16% coverage
PA14_36730 putative trehalose synthase from Pseudomonas aeruginosa UCBPP-PA14
27% identity, 16% coverage
- Impact of Growth Rate on the Protein-mRNA Ratio in Pseudomonas aeruginosa
Zhang, mBio 2023 - “...( coxA , coxB , and PA14_01310 ), glycogen biosynthesis ( glgB , glgP , PA14_36730 , and PA14_36740 ), and sulfur metabolism ( PA14_19540 , ssuB , ssuD , and PA14_19590 ). FIG1 Differentially expressed mRNAs and proteins show modest overlap. Venn diagrams show the...”
- Gene Expression Profiling of <i>Pseudomonas aeruginosa</i> Upon Exposure to Colistin and Tobramycin
Cianciulli, Frontiers in microbiology 2021 - “...44.57 10.58 PA14_36630 PA14_36630 Glycosyl hydrolase 25.56 6.53 glgB PA14_36710 Glycogen branching protein 38.34 15.21 PA14_36730 PA14_36730 Trehalose synthase 30.78 16.68 PA14_36740 PA14_36740 Hypothetical protein 4.77 5.04 glgP PA14_36840 Glycogen phosphorylase 5.98 2.93 PA14_36850 PA14_36850 Hypothetical protein 2.26 2.9 Pathogenicity and virulence tssL1 PA14_00925 Hypothetical protein...”
- “...( PA14_36570 , PA14_36580 , PA14_36590 , PA14_36605 , PA14_36620 , PA14_36630 , glgB , PA14_36730 ) were down-regulated after exposure to tobramycin ( Table 2 and Supplementary Table 1 ). Tobramycin Affects the Expression of Functions Involved in Pathogenicity, Virulence and Transport The comparative transcriptome...”
PA2152 probable trehalose synthase from Pseudomonas aeruginosa PAO1
PLES_31741 putative trehalose synthase from Pseudomonas aeruginosa LESB58
27% identity, 16% coverage
- Trehalose and α-glucan mediate distinct abiotic stress responses in Pseudomonas aeruginosa
Woodcock, PLoS genetics 2021 - “...glgE PA2151 Maltosyltransferase Extension of -glucan using M1P as the donor 2.4.99.16 treS / pep2 PA2152 Trehalose-maltose isomerase/maltokinase Isomerisation between trehalose and maltose/phosphorylation of maltose to M1P using ATP 5.4.99.16/2.7.1.175 glgB PA2153 Glycogen branching enzyme Introduction of -1,6-linked branches into -glucan 2.4.1.18 glgA PA2165 Glycogen synthase...”
- “...PCR using GoTaq (Promega) and primers listed in S2 Table . The pME3087-derivative vector p PA2152 lacks the sacB counter-selection gene. In this case, we used tetracycline enrichment to select for double crossover candidates following the method described in [ 78 ] followed by PCR confirmation...”
- Mobilization of Iron Stored in Bacterioferritin Is Required for Metabolic Homeostasis in Pseudomonas aeruginosa
Punchi, Pathogens (Basel, Switzerland) 2020 - “...by pointing to the enrichment of enzymes involved in the conversion of glycogen to glucose (PA2152 and TreA) [ 72 , 73 , 74 ] in the bfd cells. Glycogen is a major intracellular reserve polymer of the -1-4-linked glucose monomers that accumulates in bacterial cells...”
- “...75 , 76 , 77 , 78 , 79 ]. As such, the enrichment of PA2152 and TreA in the bfd cells suggests alterations in carbon metabolism. Previous studies comparing the metabolic flux of exponential phase Pseudomonas putida cells grown in iron replete vs. iron limiting...”
- High-throughput detection of RNA processing in bacteria
Gill, BMC genomics 2018 - “...aruI , PA0475, PA0558, PA1025 5 n/a 5,224,5685,224,795 PA4656.1 228 pant430 Not identified PA2038, PA3517, PA2152, pslE , PA2472, PA2750 4 3 5,283,9605,284,110 PA4704.1 1 151 prrF1 prrF1 prrF2 12 217 5,284,1725,284,319 PA4704.2 1 148 prrF2 prrF2 prrF1 20 141 5,283,9605,284,319 prrH 1 360 prrH prrH...”
- Comparative systems biology analysis to study the mode of action of the isothiocyanate compound Iberin on Pseudomonas aeruginosa
Tan, Antimicrobial agents and chemotherapy 2014 - “...et al. TABLE 3 (Continued) Gene PA2144 PA2151 PA2152 PA2153 PA2159 PA2160 PA2162 PA2163 PA2164 PA2167 PA2169 PA2170 PA2177 PA2193 PA2194 PA2195 PA2261 PA2300...”
- Pseudomonas aeruginosa Genome Evolution in Patients and under the Hospital Environment
Lucchetti-Miganeh, Pathogens (Basel, Switzerland) 2014 - “...gene. Whether this disturbs cgr -dependent cupA regulation in this strain requires functional studies. The PA2152 gene encoding a protein with maltose alpha-D-glucosyltransferase activity probably involved in trehalose biosynthesis in PAO1 strain [ 59 ] exhibits premature stop codons in both ST395 strains, possibly suggesting that...”
- Analysis of the Pseudomonas aeruginosa regulon controlled by the sensor kinase KinB and sigma factor RpoN
Damron, Journal of bacteriology 2012 - “...PA2144 PA2145 PA2146 PA2147 PA2148 PA2149 PA2150 PA2151 PA2152 PA2153 PA2154 PA2155 PA2156 PA2157 PA2158 PA2159 PA2160 PA2161 PA2162 PA2163 PA2164 PA2165 PA2167...”
- Drosophila melanogaster-based screening for multihost virulence factors of Pseudomonas aeruginosa PA14 and identification of a virulence-attenuating factor, HudA
Kim, Infection and immunity 2008 - “...California, Berkeley Genes upregulated in hudR PA0254 PA0475 PA2152 PA2935 PA3039 PA0190 PA2376 PA2534 PA0798 PA2517 PA4127 PA0816 PA1942 PA4155 PA4349 PA1145...”
- Microarray analysis of the osmotic stress response in Pseudomonas aeruginosa
Aspedon, Journal of bacteriology 2006 - “...confirmed GeneChip data for two up-regulated genes (pscC and PA2152) and one down-regulated gene (PA2260) (data not shown). These 66 genes are referred to here...”
- “...involved in the synthesis of the osmoprotectants trehalose (PA2152) and NAGGN (PA3459 to PA3461) (Table 1). Furthermore, the expression profile and genetic...”
- More
- Recombination is a key driver of genomic and phenotypic diversity in a Pseudomonas aeruginosa population during cystic fibrosis infection
Darch, Scientific reports 2015 - “...3378321 pslJ synonymous 7 0.002 3478510 PLES_31551 - hypothetical Leu to Phe 2 1.098 3502354 PLES_31741 trehalose synthase Leu to Val 2 1.049 3973880 Intergenic PLES_35761 - cysB NA 5 4015334 pcrV Asp to Asn 2 1.104 4026225 pscT Asn to Lys 4 1.088 4539068 PLES_41121...”
SSGG_05057 maltose alpha-D-glucosyltransferase from Streptomyces filamentosus NRRL 15998
27% identity, 36% coverage
- Transcriptional analysis of the effect of exogenous decanoic acid stress on Streptomyces roseosporus
Liao, Microbial cell factories 2013 - “...3 ). Expression of genes coding for the putative maltose ABC transporter (SSGG_01377), and Tres (SSGG_05057) which catalyzes the conversion of maltose into Trehalose was elevated. In addition, gene expression of alpha-amylase (SSGG_05058) was induced, which degrade starch to provide the maltose for synthesis of trehalose....”
- “...to control Locus Gene description Log 2 stress/control SSGG_01377 putative maltose ABC transporter permease 2.46 SSGG_05057 trehalose synthase 3.09 SSGG_05058 alpha-amylase 2.12 SSGG_03685 fructose-bisphosphate aldolase 1.37 SSGG_06343 phosphopyruvate hydratase 1.63 SSGG_01114 pyruvate kinase 1.30 SSGG_02477 phosphopyruvate hydratase 1.53 Genes encoding proteins involved in the TCA cycle...”
BCAL_RS02400 glycoside hydrolase family 13 protein from Bifidobacterium callitrichos DSM 23973
27% identity, 33% coverage
- The comparative genomics of Bifidobacterium callitrichos reflects dietary carbohydrate utilization within the common marmoset gut
Albert, Microbial genomics 2018 - “...phosphorylase 2.4.1.7 COO72_RS10000 CPA40_RS07310 BCAL_RS06670 UTPglucose-1-phosphate uridylyltransferase 2.7.7.9 COO72_RS10475 CPA40_RS09980 BCAL_RS08755 -Amylase 3.2.1.1 COO72_RS11340 CPA40_RS02640 BCAL_RS02400 -Glucosidase 3.2.1.21 COO72_RS09740 CPA40_RS01270 BCAL_RS01445 Isoamylase 3.2.1.68 COO72_RS04240 CPA40_RS05215 BCAL_RS04710 Glucose-1-phosphate adenylyltransferase 2.7.7.27 COO72_RS01125 CPA40_RS05455 BCAL_RS09705 1,4--Glucan branching enzyme 2.4.1.18 COO72_RS03710 CPA40_RS0112 BCAL_RS04065 4--Glucanotransferase 2.4.1.25 COO72_RS08655 CPA40_RS05200 BCAL_RS04695 Glycogen phosphorylase...”
VZ55_RS04715 alpha-glucosidase from Gluconobacter oxydans
27% identity, 42% coverage
SVEN_5096 maltose alpha-D-glucosyltransferase from Streptomyces venezuelae ATCC 10712
27% identity, 34% coverage
D1ABU6 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Thermomonospora curvata (see 2 papers)
27% identity, 31% coverage
A0A2Z4RCL2 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Pseudomonas putida (see paper)
26% identity, 16% coverage
ArtHe_17800 maltose alpha-D-glucosyltransferase from Arthrobacter sp. Helios
27% identity, 29% coverage
BAE86926.1 α-glucosidase I (Hbg1;HBG-I;Loc409889) (EC 3.2.1.20) (see protein)
NP_001035326 alpha glucosidase 2 precursor from Apis mellifera
Q25BT8 alpha-glucosidase from Apis mellifera
28% identity, 38% coverage
- Nosema ceranae escapes fumagillin control in honey bees
Huang, PLoS pathogens 2013 - “...Corresponded protein in honey bee genome 1, 2, 3, 13, 14, 22, 23, 29, 30 NP_001035326 alpha glucosidase 2 [ Apis mellifera ] 4 XP_393509 PREDICTED: mitochondrial-processing peptidase subunit beta-like [ Apis mellifera ] 5 XP_392899 PREDICTED: 60 kDa heat shock protein, mitochondrial-like [ Apis mellifera...”
- Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1
Plaza-Vinuesa, Microbial cell factories 2019 - “...accession Q9ZEU2), GH13_16 ( Propionibacterium freudenreichii subsp. Shermanii , A1XGB1), GH13_17 ( Apis mellifera , Q25BT8), GH13_18 ( Bifidobacterium adolescentis , Q84HQ2), GH13_20 ( Lactobacillus plantarum WCFS1, F9URM8), GH13_21 ( Escherichia coli , P21517), GH13_23 ( Xhantomonas campestris , Q76LB0), GH_29 ( Bacillus subtilis , P39795),...”
- Comprehensive enzymatic analysis of the amylolytic system in the digestive fluid of the sea hare, Aplysia kurodai: Unique properties of two α-amylases and two α-glucosidases
Tsuji, FEBS open bio 2014 - “...DrMAL1 from Drosophila virilis (UnitProtKB, O16098 ); -glucosidase HbAGL1 (UnitProtKB, Q17058 ) and HbAGL2 (UniProtKB, Q25BT8 ) from honey bee, Apis mellifera ; oligo-1,6-glucosidase GtOG from Geobacillus thermoglucosidasius (UnitProtKB, P29094 ); and sucrase PaSUC from pea aphid, Acrythosiphon pisum (UniProtKB, Q0H3F1 ). The amino-terminal sequence of...”
PputUW4_02800 maltose alpha-D-glucosyltransferase from Pseudomonas sp. UW4
26% identity, 16% coverage
- The complete genome sequence of the plant growth-promoting bacterium Pseudomonas sp. UW4
Duan, PloS one 2013 - “...identified. The TreS pathway involves the conversion of maltose to trehalose by trehalose synthase (TreS) (PputUW4_02800). In the TreY-TreZ pathway, maltodextrin is first converted to maltooligosyltrehalose by maltooligosyltrehalose synthase (TreY) (PputUW4_02792), and then to trehalose by maltooligosyltrehalose trehalohydrolase (TreZ) (PputUW4_02790). When searching the orthologs in the...”
CAA34072.1 α-amylase 3 (AmyC) (EC 3.2.1.1) (see protein)
23% identity, 44% coverage
S1RS09 Neopullulanase from Enterococcus cecorum DSM 20682 = ATCC 43198
30% identity, 17% coverage
- Long-term Immunity of a Microneedle Array Patch of SARS-CoV-2 S1 Protein Subunit Vaccine Irradiated by Gamma Rays in Mice.
Kim, bioRxiv : the preprint server for biology 2024 - “...expression in mammalian cells [ 32 , 33 ] and cloned into pAdlox. Similarly, for S1RS09, spanning amino acids 1 to 661 and equipped with the Bam HI-RS09 (APPHALS, TLR4 agonist)-EPEA, synthesis and cloning into pAdlox were performed using the same method [ 31 ]. For...”
- “...(Delta rS1, rS1RS09, rS1f, and rS1fRS09) were successfully purified for subsequent experiments. 3.2. Identification of S1RS09 as an optimal immunogen To compare the immunogenicity of four Delta S1 proteins, BALB/c mice (68 weeks old, n = 5) were immunized intramuscularly (IM) with 45 g of either...”
B1PK99 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Meiothermus ruber (see 4 papers)
27% identity, 18% coverage
BTHUR0008_RS01820 alpha-glucosidase from Bacillus thuringiensis serovar berliner ATCC 10792
24% identity, 43% coverage
BAD_RS08195 alpha-amylase family glycosyl hydrolase from Bifidobacterium adolescentis ATCC 15703
26% identity, 32% coverage
- Combining of transcriptome and metabolome analyses for understanding the utilization and metabolic pathways of Xylo-oligosaccharide in Bifidobacterium adolescentis ATCC 15703
Yang, Food science & nutrition 2019 - “...BAD_RS07400 2.44 BGAL16 Betagalactosidase 70.88 13.06 ko01100/ko00052/ko00600/ko00511 BAD_RS01040 2.42 gnd 6phosphogluconate dehydrogenase 240.52 45.07 ko01100/ko01110/ko01130/ko01120//ko01200/ko00030/ko00480 BAD_RS08195 2.20 malL Alphaamylase 3,359.55 733.05 ko01100/ko00500/ko00052 BAD_RS02270 2.08 xynB Betaxylosidase 1,066.15 251.3 ko01100/ko00500/ko00052 BAD_RS08325 1.97 LacZ Betagalactosidase 98.72 25.2 ko01100/ko00052/ko00600/ko00511 BAD_RS02150 1.68 fucO Lactaldehyde reductase 5,736.74 1,795.21 ko01120/ko00630/ko00640 BAD_RS08455 1.38...”
- “...the action of xylose isomerase and xylulose kinase. The betaxylosidase (encoded BAD_RS02270) and alphaamylase (encoded BAD_RS08195) involved in KEGG pathway (ko00500 and ko00052) were upregulated. The upregulated genes were associated with some metabolites, including xylulose kinase, xylosidase, xylose isomerase, xylose proton symporter, which may pertain to...”
P14899 Alpha-amylase 3 from Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
23% identity, 43% coverage
Q5SL15 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Thermus thermophilus (see paper)
26% identity, 18% coverage
VP0711 trehalose-6-phosphate hydrolase from Vibrio parahaemolyticus RIMD 2210633
VP_RS03410 alpha,alpha-phosphotrehalase from Vibrio parahaemolyticus RIMD 2210633
22% identity, 39% coverage
SCO2228 alpha-glucosidase from Streptomyces coelicolor A3(2)
28% identity, 32% coverage
- Acarbose May Function as a Competitive Exclusion Agent for the Producing Bacteria
Tanoeyadi, ACS chemical biology 2023 - “...from S. coelicolor A(3)2, both in size and domain organization, whereas GacE1 is similar to SCO2228 (GH13_30) (another putative -amylase from S. coelicolor A3(2)). Therefore, GacE2 and TresE may be derived from the same ancestral protein as SCO7020 and GacE1 may come from the same origin...”
- “...to be sensitive to acarbose ( Figure 6b ). On the other hand, GacE1 and SCO2228 lack a signal peptide, suggesting that they are not secreted into the environment. While the size of AcbE, AcbZ, TresZ, GacZ1, and GacZ2 (~1050 aa) are similar to SCO7019 (993...”
- The Inhibition of Antibiotic Production in Streptomyces coelicolor Over-Expressing the TetR Regulator SCO3201 IS Correlated With Changes in the Lipidome of the Strain
Zhang, Frontiers in microbiology 2020 - “...Starch and sucrose metabolism sco0765 Secreted endoglucanase 0 1.092 sco1879 Putative secreted pectinesterase 9.994 0 sco2228 Alpha-glucosidase 1.867 2.593 sco2649 Putative 4-alpha-glucanotransferase 1.955 1.337 sco3780 Putative threalose 6 phosphate hydrolase 1.297 0 sco4285 Putative sugar kinase 1.308 0 sco4288 Putative threalose 6 phosphate phosphatase 2.402 0...”
- GlnR and PhoP Directly Regulate the Transcription of Genes Encoding Starch-Degrading, Amylolytic Enzymes in Saccharopolyspora erythraea
Xu, Applied and environmental microbiology 2016 - “...-7020 [aml and amlB]) and maltose-utilizing cluster genes (SCO2228 to SCO2232 [aglA-malGFE and malR]) in Streptomyces coelicolor A3(2) was found to possess...”
- “...-7020 [aml and amlB]) and maltose-utilizing cluster genes (SCO2228 to SCO2232 [aglAmalGFE and malR]) in Streptomyces coelicolor A3(2) harbor the putative GlnR...”
I3WCP4 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Pseudomonas sp. P8005 (see paper)
26% identity, 15% coverage
SACE_1668 alpha-glucosidase from Saccharopolyspora erythraea NRRL 2338
26% identity, 35% coverage
Achr_e950 alpha-glucosidase from Azotobacter chroococcum NCIMB 8003
25% identity, 37% coverage
C289_2139 alpha-glucosidase from Anoxybacillus ayderensis
26% identity, 36% coverage
- A high molecular-mass Anoxybacillus sp. SK3-4 amylopullulanase: characterization and its relationship in carbohydrate utilization
Kahar, International journal of molecular sciences 2013 - “...enzymes include amylopullulanase, ApuASK (C289_2785), -amylase, ASKA (C289_0468), -glucosidase (C289_0469), type I pullulanase (C289_2260), glycosidase (C289_2139), and oligo-1,6-glucosidase (C289_0857, C289_1909, and C289_2139). Several putative sugar transporters (C289_0465, C289_0466, C289_0467, C289_0603, C289_0763, C289_0764, C289_0765, C289_0778, C289_0779, C289_0780, C289_1015, C289_1174, C289_1392, C289_1394, C289_1910, C289_1911, and C289_1912) were also...”
LMOf2365_0270 oligo-1,6-glucosidase from Listeria monocytogenes str. 4b F2365
25% identity, 41% coverage
Q7WUI5 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Thermus thermophilus (see 2 papers)
27% identity, 17% coverage
A0A1S7D3W6 alpha-amylase (EC 3.2.1.1) from Microbacterium foliorum (see paper)
26% identity, 33% coverage
CNF00610 alpha-glucosidase from Cryptococcus neoformans var. neoformans JEC21
25% identity, 31% coverage
PSPTO2761 alpha-amylase family protein from Pseudomonas syringae pv. tomato str. DC3000
26% identity, 16% coverage
Q47SE5 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Thermobifida fusca (see paper)
27% identity, 31% coverage
LOC111049618 maltase A3 from Nilaparvata lugens
25% identity, 30% coverage
- Transcriptome and metabolome analyses reveal the responses of brown planthoppers to RH resistant rice cultivar
Li, Frontiers in physiology 2022 - “...LOC111046284, insulin-like peptide; LOC111050834, uncharacterized protein; LOC111053763, acetylcholine receptor; LOC111045895, keratin-associated protein; LOC111048221, ATP-citrate synthase; LOC111049618, maltase; LOC111054483, sodium channel protein; LOC111064163, heat shock protein; LOC111052384, pistil-specific extensin-like protein; LOC111043756, uncharacterized protein; LOC111050339, uncharacterized protein; LOC111052663, putative serine protease. The values are presented as the mean...”
Psyr_2490 Alpha amylase, catalytic region from Pseudomonas syringae pv. syringae B728a
26% identity, 16% coverage
MXAN_3684 trehalose synthase from Myxococcus xanthus DK 1622
28% identity, 31% coverage
ID870_08450 alpha,alpha-phosphotrehalase from Streptococcus agalactiae CJB111
26% identity, 35% coverage
- Identification of a DNA-cytosine methyltransferase that impacts global transcription to promote group B streptococcal vaginal colonization
Manzer, mBio 2023 - “...that were most dysregulated were those involved in sugar metabolism or transport, including treP/C (ID870_08455, ID870_08450) , dhaK/L/M (ID870_01490ID870_01480) , lrgA/B (ID870_08490, ID870_08485) , lacA/B (ID870_00285, ID870_00290) , argF (ID870_10075) , arcC (ID870_10080) , and multiple genes belonging to pts sugar transporters. At the early stationary...”
- “...name Description EXP fold change ES fold change Metabolism ID870_08455 treP Trehalose phosphorylase 69.17 2.75 ID870_08450 treC Trehalose-6-phosphate hydrolase 66.48 3.00 ID870_00285 lacA Galactose-6-phosphate subunit A 4.12 5.40 ID870_00290 lacB Galactose-6-phosphate subunit B 3.42 2.82 ID870_01490 dhaK Dihydroxyacetone kinase subunit K 9.46 18.97 ID870_01485 dhaL Dihydroxyacetone...”
VpaChn25_0669 alpha,alpha-phosphotrehalase from Vibrio parahaemolyticus
23% identity, 32% coverage
F1QEA9 Neutral and basic amino acid transport protein rBAT from Danio rerio
28% identity, 23% coverage
SAK_0258 alpha amylase family protein from Streptococcus agalactiae A909
26% identity, 35% coverage
BC0413 Exo-alpha-1,4-glucosidase from Bacillus cereus ATCC 14579
26% identity, 35% coverage
EfmE1162_1270 alpha-glucosidase from Enterococcus faecium E1162
26% identity, 35% coverage
- A LacI-family regulator activates maltodextrin metabolism of Enterococcus faecium
Zhang, PloS one 2013 - “...the prediction that MdxR regulates the transcription of this cluster of genes. Three other genes (EfmE1162_1270, EfmE1162_1401, and EfmE1162_1402, which are not located in the immediate vicinity of the mdxABCD - pulA gene cluster were also expressed at lower levels in the mdxR deletion mutant. EfmE1162_1270...”
- “...in the breakdown of maltodextrin in B. subtilis 168 [ 18 ] which suggests that EfmE1162_1270 and EfmE1162_1401 also have a role in the metabolism of maltodextrins in E. faecium . EfmE1162_1402 is predicted to encode a -glucosidase that hydrolyzes the -1,4 bonds of sugars like...”
DKG71_32885 glycoside hydrolase family 13 protein from Streptomyces sp. NEAU-S7GS2
25% identity, 34% coverage
- Antifungal, Plant Growth-Promoting, and Genomic Properties of an Endophytic Actinobacterium Streptomyces sp. NEAU-S7GS2
Liu, Frontiers in microbiology 2019 - “...plates ( Supplementary Figures S4C,D ), and two glucanase (-1,3-glucanase: DKG71_10585; endoglucanase: DKD71_15870), one -amylase (DKG71_32885) and four glucoamylase (DKG71_10575, DKG71_10960, DKD71_31240, DKG71_31870) genes were identified in the genome of NEAU-S7GS2. Although there are 48 protease (such as DKG71_41140, DKG71_39880, DKG71_37495) and five chitinase (DKG71_41330, DKG71_38830,...”
BAA12704.1 α-glucosidase / exo-α-1,4-glucosidase (EC 3.2.1.20) (see protein)
25% identity, 35% coverage
BWI76_RS03235 Neopullulanase (EC 3.2.1.135) from Klebsiella michiganensis M5al
cymH / Q48398 cyclodextrinase (EC 3.2.1.54) from Klebsiella oxytoca (see 4 papers)
CAA60007.1 cyclodextrinase (CymH) (EC 3.2.1.54) (see protein)
Q48398 CymH protein from Klebsiella oxytoca
29% identity, 16% coverage
- mutant phenotype: Specifically important for utilizing a-Cyclodextrin. Automated validation from mutant phenotype: the predicted function (3.2.1.135) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
- Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1
Plaza-Vinuesa, Microbial cell factories 2019 - “...GH13_20 representatives from Alicyclobacillus acidocaldarius (AAC) (accession Q9WX32), Thermoactinomyces vulgaris (TVU) (Q08751), Klebsiella oxytoca (KOX) (Q48398), Bacillus sp. I-5 (BSP) (Q59226), Lysinibacillus sphaericus (LSP) (Q08341), Anoxybacillus flavithermus (AFL) (Q5BLZ6), Geobacillus caldoxylosilyticus (GCA) (CoLZ63), Thermus sp. IM6501 (O69007), Bacillus stearothermophilus (BST) (P38940), and Lactobacillus plantarum WCFS1 (LPL)...”
5m99A / A0A059TXD8 Functional characterization and crystal structure of thermostable amylase from thermotoga petrophila, reveals high thermostability and an archaic form of dimerization (see paper)
25% identity, 35% coverage
- Ligand: calcium ion (5m99A)
2ze0A / Q33E90 Alpha-glucosidase gsj (see paper)
24% identity, 37% coverage
- Ligand: calcium ion (2ze0A)
EF1347 glycosyl hydrolase, family 13 from Enterococcus faecalis V583
29% identity, 18% coverage
- Transcriptional response of Enterococcus faecalis V583 to erythromycin
Aakra, Antimicrobial agents and chemotherapy 2005 - “...EF2305 EF2704 EF2705 EF2736 EF3113 EF0956 EF0957 EF1071 EF1347 EF1348 EF1349 EF1411 EF1543 EF1606 EF2646 EF0283 EF0848 EF1608 EF2691 EF2883 EF3112 EF2320 0 0...”
- “...EF0900 EF0939 EF0956 EF0957 EF1020 EF1068 EF1109 EF1211 EF1347 EF1348 EF1349 EF1526 EF1612 EF1613 EF1826 EF2589 EF2646 EF2863 EF2961 EF3157 EF0169 EF0514 EF0913...”
- Resistance to linezolid: characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci
Prystowsky, Antimicrobial agents and chemotherapy 2001 - “...of VRE, 5 of which were Enterococcus faecium (EF208, EF1347, EF1401, EF1509, and EF1644), 4 of which were Enterococcus faecalis (F118, F177, F217, and F317),...”
- “...1 E. gallinarum Z393 1 1 2 2 4 8 8 8 8 8 8 8 8 E. faecium EF208 EF1347 EF1401 EF1509 EF1644 1 1 1 1 1 2 2 2 2 2 2 4 2 2 2 2 4 2 2 2 2 4 2 2 2 4 4 2 2 2 4 4...”
KAR53_01435 alpha,alpha-phosphotrehalase from Periweissella ghanensis
24% identity, 34% coverage
FORC16_3686 alpha-glucosidase from Vibrio vulnificus
23% identity, 38% coverage
A5ILB0 alpha-amylase (EC 3.2.1.1) from Thermotoga petrophila (see paper)
25% identity, 33% coverage
Dtur_1675 alpha amylase catalytic region from Dictyoglomus turgidum DSM 6724
21% identity, 43% coverage
- The Complete Genome Sequence of Hyperthermophile Dictyoglomus turgidum DSM 6724™ Reveals a Specialized Carbohydrate Fermentor
Brumm, Frontiers in microbiology 2016 - “...-amylase COCOR_00322 39.7% Dtur_0857 GH 53 -galactanase TRQ7_08325 56.5% Dtur_1586 GH 5 cellulase BSONL12_10711 41.5% Dtur_1675 GH 13 -amylase CAAU_0986 51.6% Dtur_1715 GH 10 xylanase Pmob_0231 46.9% Dtur_1729 GH 43 -xylosidase Csac_1560 67.9% Dtur_1739 GH 51 -xylosidase Calhy_1625 58.9% Dtur_1740 GH 39 -xylosidase TRQ7_03440 38.3% Table...”
- “.... The genome of D. turgidum codes for three annotated extracellular -amylases (Dtur_0675; Dtur_0676, and Dtur_1675) as well as four annotated intracellular -amylases (Dtur_0770; Dtur_0794; Dtur_0895, and Dtur_0896) and six annotated -glucosidases (Dtur_0157; Dtur_0171; Dtur_0320; Dtur_0384; Dtur_0490, and Dtur_1749). These intracellular enzymes may function in both...”
palQ / Q9AI62 palatinase (EC 3.2.1.219) from Erwinia rhapontici (see paper)
PALQ_ERWRD / Q9AI62 Palatinase; EC 3.2.1.219 from Erwinia rhapontici (Pectobacterium rhapontici) (see paper)
AAK28737.1 oligo-α-glucosidase / palatinase (PalQ) (EC 3.2.1.10|3.2.1.-) (see protein)
24% identity, 36% coverage
- function: Catalyzes the hydrolysis of palatinose (PubMed:11274100). Shows a strict specificity toward palatinose, and cannot release glucose from the disaccharides sucrose, maltose, trehalose and melibiose (PubMed:11274100). Involved in the degradation of palatinose, a sucrose isomer that is formed as a reserve material under conditions of excess carbon availability, sequestered in a form unavailable to competitors such as fungi or the host plant, and whose consumption appears to be postponed until the preferentially metabolized carbon source (e.g. sucrose) is depleted (PubMed:11274100).
catalytic activity: 6-O-alpha-D-glucopyranosyl-D-fructose + H2O = alpha-D-glucose + D-fructose (RHEA:68808)
Q33E90 alpha-glucosidase (EC 3.2.1.20) from Geobacillus sp. HTA-462 (see paper)
BAE48285.1 α-glucosidase (Gsj) (EC 3.2.1.20) (see protein)
24% identity, 35% coverage
6y9tA / Q5FI02 Family gh13_31 enzyme (see paper)
26% identity, 31% coverage
- Ligand: calcium ion (6y9tA)
Gocc_0097 maltose alpha-D-glucosyltransferase from Gaiella occulta
31% identity, 29% coverage
lp_3627 alpha-glucosidase from Lactobacillus plantarum WCFS1
lp_3627 alpha-glucosidase from Lactiplantibacillus plantarum WCFS1
25% identity, 35% coverage
LBA1872 oligo-1,6-glucosidase from Lactobacillus acidophilus NCFM
26% identity, 30% coverage
- An 1,4-α-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus
Andersen, Applied and environmental microbiology 2020 (secret) - Enzymology and structure of the GH13_31 glucan 1,6-α-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM
Møller, Journal of bacteriology 2012 - “...sequences from BLASTP searches using LBA0264 and LBA1872 were retrieved and complemented with lactobacillus sequences from CAZy (8) annotated as GH13_31....”
- “...transport and catabolism gene cluster (LBA1866, LBA1867, and LBA1872) (34), and L. acidophilus NCFM 16S rRNA (LBA2071) transcripts, whose expression is always...”
- Microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in Lactobacillus acidophilus
Azcarate-Peril, Applied and environmental microbiology 2005 - “...transcriptional repressor (fructose operon) LBA1870 maltose phosphorylase LBA1872 oligo-1,6-glucosidase 1.31 0.07 0.17 0.98 1.00 0.92 0.67 0.97 3.22 0.17 0.43...”
VC0911 trehalose-6-phosphate hydrolase from Vibrio cholerae O1 biovar eltor str. N16961
23% identity, 32% coverage
- Cross-Platform Transcriptomic Data Integration, Profiling, and Mining in Vibrio cholerae
Qin, Microbiology spectrum 2023 - “...most significantly upregulated at the overall transcriptome level were vc2698 , vca1031 , vc0910 , vc0911 , and vc2699 . The gene vc2698 ( aspA ), an aspartate ammonia-lyase, was identified in a previous study ( 11 ) as being responsible for the reversible conversion of...”
- “...vc0910 ( treB ) was identified as phosphotransferase system (PTS) trehalose transporter subunit IIBC and vc0911 ( treC ) was identified as ,-phosphotrehalase; both of these belong to the PTS system, which is essential for the colonization of V. cholerae , especially in the host (...”
- Transcriptional profiling of Vibrio cholerae O1 following exposure to human anti- lipopolysaccharide monoclonal antibodies
Baranova, Pathogens and disease 2020 - “...changed following ZAC-3 IgG treatment. log2a VCA1028 VC0911 VCA0247 VC1649 VC0910 VCA0860 VCA0945 VCA0908 VCA0944 VC0606 VCA0136 VCA0137 VC0089 VC1778 VC0795...”
- RadD Contributes to R-Loop Avoidance in Sub-MIC Tobramycin
Negro, mBio 2019 - “...46 9.0 0.022 VC1044 Unknown 622 53 11.7 0.038 VC0300 Unknown 271 16 16.9 0.015 VC0911 treA Trehalose metabolism 331 31 10.8 0.032 VC2669 Tyrosine metabolism 462 78 5.9 0.000 VC0395 gtaB Carbohydrate metabolism 446 53 8.4 0.043 VC0964 crr Carbohydrate metabolism 341 72 4.7 0.004...”
Exig_2537 alpha amylase catalytic region from Exiguobacterium sibiricum 255-15
24% identity, 35% coverage
- Engineering of Thermal Stability in a Cold-Active Oligo-1,6-Glucosidase from Exiguobacterium sibiricum with Unusual Amino Acid Content
Berlina, Biomolecules 2021 - “...expression of several predicted glucosidases was found to be influenced by temperature including Exig_1739 and Exig_2537 genes encoding for the putative alpha-amylases [ 32 ]. Expression of the Exig_1739 gene increased at 2.5 C, while the expression of the Exig_2537 gene was downregulated at this temperature...”
- “...revealed decreased number of arginine residues and more glycine, lysine, and isoleucine as compared to Exig_2537. Such features are characteristic of cold-active enzymes in comparison with mesophilic and thermophilic homologs [ 44 ]. These data suggest that E. sibiricum genome contains genes encoding mesophilic and psychrophilic...”
- Architecture of thermal adaptation in an Exiguobacterium sibiricum strain isolated from 3 million year old permafrost: a genome and transcriptome approach
Rodrigues, BMC genomics 2008 - “...For instance, the alpha-amylase encoded by the gene Exig_1739 was up regulated at -2.5C, while Exig_2537 was down regulated at -2.5C and slightly up-regulated at 10C and 40C when compared to 28C [see Additional file 1 : Table S2]. The analysis of these proteins showed that...”
- “...residues (27) and more glycine (37 residues), lysine (25 residues), and isoleucine residues (35) than Exig_2537 (35, 32, 15, 27 residues, respectively). These protein features are some of the characteristic changes found in cold-active enzymes relative to their mesophilic and thermophilic counterparts in alpha-amylases and in...”
treA / P39795 trehalose-6-phosphate hydrolase (EC 3.2.1.93) from Bacillus subtilis (strain 168) (see paper)
TREC_BACSU / P39795 Trehalose-6-phosphate hydrolase; Alpha,alpha-phosphotrehalase; Phospho-alpha-(1-1)-glucosidase; EC 3.2.1.93 from Bacillus subtilis (strain 168) (see 2 papers)
CAB12610.1 trehalose-6-phosphate hydrolase (TreA;BSU07810) (EC 3.2.1.93) (see protein)
BSU07810 trehalose-6-phosphate hydrolase from Bacillus subtilis subsp. subtilis str. 168
25% identity, 39% coverage
- function: Hydrolyzes trehalose-6-phosphate to glucose and glucose 6- phosphate. Can also very effectively hydrolyze p-nitrophenyl-alpha-D- glucopyranoside, but not lactose, maltose, sucrose or sucrose-6- phosphate. Trehalose is also hydrolyzed, but to a much smaller extent than trehalose-6-phosphate.
catalytic activity: alpha,alpha-trehalose 6-phosphate + H2O = D-glucose 6- phosphate + D-glucose (RHEA:23008) - Unravelling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1
Plaza-Vinuesa, Microbial cell factories 2019 - “...Escherichia coli , P21517), GH13_23 ( Xhantomonas campestris , Q76LB0), GH_29 ( Bacillus subtilis , P39795), GH13_30 ( Thermonospora curvata , Q60027), GH13_31 ( Bacillus cereus , P21332), GH13_34 ( Xenopus leavis , Q7ZYR3), GH13_35 ( Xenopus leavis , Q32NL8), GH13_36 ( Paenibacillus polymyxa E681, E0RLH8)...”
- Reconstruction of the Regulatory Network for Bacillus subtilis and Reconciliation with Gene Expression Data
Faria, Frontiers in microbiology 2016 - “...D -fructose-1, 6-bisphosphate, Glucose-6-Phosphate, phosphate, D-trehalose-6-phosphate 0.807 PTS system, trehalose-specific IIB component (EC 2.7.1.69) treA, BSU07810 D -fructose-1, 6-bisphosphate, Glucose-6-Phosphate, phosphate, D-trehalose-6-phosphate 0.814 Trehalose-6-phosphate hydrolase (EC 3.2.1.93) treR, BSU07820 D -fructose-1, 6-bisphosphate, Glucose-6-Phosphate, phosphate, D-trehalose-6-phosphate 0.734 Trehalose operon transcriptional repressor yfkO, BSU07830 D isulfide_stress_conditions 0.706 Oxygen-insensitive...”
- The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis
Eichenberger, PLoS biology 2004 - “...spoVK (BSU17420), spoVM (BSU15810), spsA (BSU37910), spsG (BSU37850), spsJ (BSU37830), tenI (BSU11660), thiS (BSU11680), treA (BSU07810), treR (BSU07820), uxuA (BSU12340), ybaN (BSU01570), ybaS (BSU01590), ycgF (BSU03090), ycgM (BSU03200), ycgN (BSU03210), ydcI (BSU04780), ydhF (BSU05730), yeeA (BSU06760), yeeB (BSU06770), yeeC (BSU06780), yefA (BSU06730), yefB (BSU06740), yefC (BSU06750),...”
CNAG_05913 alpha-glucosidase from Cryptococcus neoformans var. grubii H99
24% identity, 33% coverage
- Identification of Pathogen Genomic Differences That Impact Human Immune Response and Disease during Cryptococcus neoformans Infection
Gerstein, mBio 2019 - “...CNAG_04535 2.79 0.095 CNAG_04922 9.97 0.0016 CNAG_05662 ( itr4 ) 6.22 0.013 CNAG_05663 0.61 0.43 CNAG_05913 0.07 0.79 CNAG_05937 0.09 0.77 CNAG_06169 0.13 0.72 CNAG_06332 4.05 0.044 CNAG_06490 1.02 0.31 CNAG_06574 ( app1 ) 9 0.0027 CNAG_06704 5.83 0.016 CNAG_06876 0.05 0.82 CNAG_06986 7 0.0082 CNAG_07703...”
- “...strains in the following genes deleted: Experiment 1 (E1)CNAG_00363, CNAG_02176, CNAG_04373, CNAG_04535, CNAG_04922, CNAG_05662, CNAG_05663, CNAG_05913, CNAG_06169, CNAG_06332, CNAG_06574, CNAG_06704, CNAG_06876, and CNAG_07837; Experiment 2 (E2)CNAG_05973, CNAG_06490, CNAG_06986; Experiment 3 (E3)CNAG_07703 ( 35 ). For E1, five C57BL/6 mice per group were anesthetized by intraperitoneal pentobarbital...”
LLKF_1606 glucan 1,6-alpha-glucosidase from Lactococcus lactis subsp. lactis KF147
22% identity, 39% coverage
MAL1_DROME / P07190 Maltase A1; Larval visceral protein H; EC 3.2.1.20 from Drosophila melanogaster (Fruit fly) (see paper)
AAF59089.3 α-glucosidase H (LvpH; RE72980p) (EC 3.2.1.20) (see protein)
27% identity, 32% coverage
- catalytic activity: Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D- glucose residues with release of alpha-D-glucose.
- Temporal regulation of proteome profile in the fruit fly, Drosophila melanogaster
Subramanian, PeerJ 2016 - “...10 21 3 32 N-glycanase Q28YQ7 73443 8.15 26 1 3 4 41 Maltase H P07190 66344 4.75 82 5 10 5 44 Vacuolar ATP synthase catalytic subunit A Q27331 68259 5.23 148 13 22 6 51 Vacuolar ATP synthase subunit B P31409 54515 5.25 131...”
- “...18 41 165 ATP synthase subunit P35381 59384 9.09 196 5 14 42 166 Maltase P07190 66344 4.75 92 7 15 43 170 Pyruvate kinase O62619 57404 7.13 35 2 4 44 171 Glycerol-3-phosphate dehydrogenase Q27556 38298 6.33 27 6 20 45 173 ATP synthase subunit...”
LACR_1848 alpha-glucosidase from Lactococcus lactis subsp. cremoris SK11
26% identity, 38% coverage
Dret_0035 trehalose synthase from Desulfohalobium retbaense DSM 5692
26% identity, 15% coverage
- Complete genome sequence of Desulfohalobium retbaense type strain (HR(100))
Spring, Standards in genomic sciences 2010 - “...malto-oligosyltrehalose synthase (Dret_0039) and malto-oligosyltrehalose trehalohydrolase (Dret_0037) are expressed. On the other hand, the gene Dret_0035 encodes a trehalose synthase that can transform maltose directly into trehalose and vice versa, so that an excess of trehalose can be converted to glycogen again. The second osmotic solute...”
AAK28739.1 α-glucosidase (PalZ) (EC 3.2.1.20) (see protein)
26% identity, 35% coverage
Q835M9 Glucan 1,6-alpha-glucosidase, putative from Enterococcus faecalis (strain ATCC 700802 / V583)
EF1348 glucan 1,6-alpha-glucosidase, putative from Enterococcus faecalis V583
EF_1348 alpha-glucosidase from Enterococcus faecalis V583
24% identity, 40% coverage
- Comparative proteomics analysis of biofilms and planktonic cells of Enterococcus faecalis and Staphylococcus lugdunensis with contrasting biofilm-forming ability
Cho, PloS one 2024 - “...Q832Z3 EF_0180 ABC transporter, permease protein 17.04 19.20 0.89 Q835M8 EF_1349 Alpha-amylase 16.73 18.92 0.88 Q835M9 EF_1348 Glucan 1,6-alpha-glucosidase, putative 17.35 19.66 0.88 S . lugdunensis A0A292DIE8 EQ812_06280 Cold shock protein CspA 25.22 22.34 1.13 A0A133Q308 EQ812_07575 Tautomerase 23.65 21.00 1.13 A0A292DIK1 metE 5-methyltetrahydropteroyltriglutamatehomocysteine S-methyltransferase 23.57...”
- Use of recombinase-based in vivo expression technology to characterize Enterococcus faecalis gene expression during infection identifies in vivo-expressed antisense RNAs and implicates the protease Eep in pathogenesis
Frank, Infection and immunity 2012 - “...days 2h EF0082 EF0246 EF0402 EF0721 EF0722 EF0798 EF1348 EF1591 EF1755 EF1809 EF1826 EF1918 EF1962 EF1978 EF2207 EF2380 EF2570 EF2668 EF2744 EF2889 EF3008...”
- Transcriptional response of Enterococcus faecalis V583 to erythromycin
Aakra, Antimicrobial agents and chemotherapy 2005 - “...EF2305 EF2704 EF2705 EF2736 EF3113 EF0956 EF0957 EF1071 EF1347 EF1348 EF1349 EF1411 EF1543 EF1606 EF2646 EF0283 EF0848 EF1608 EF2691 EF2883 EF3112 EF2320 0 0 0...”
- “...EF0939 EF0956 EF0957 EF1020 EF1068 EF1109 EF1211 EF1347 EF1348 EF1349 EF1526 EF1612 EF1613 EF1826 EF2589 EF2646 EF2863 EF2961 EF3157 EF0169 EF0514 EF0913 EF0302...”
- Comparative proteomics analysis of biofilms and planktonic cells of Enterococcus faecalis and Staphylococcus lugdunensis with contrasting biofilm-forming ability
Cho, PloS one 2024 - “...EF_0180 ABC transporter, permease protein 17.04 19.20 0.89 Q835M8 EF_1349 Alpha-amylase 16.73 18.92 0.88 Q835M9 EF_1348 Glucan 1,6-alpha-glucosidase, putative 17.35 19.66 0.88 S . lugdunensis A0A292DIE8 EQ812_06280 Cold shock protein CspA 25.22 22.34 1.13 A0A133Q308 EQ812_07575 Tautomerase 23.65 21.00 1.13 A0A292DIK1 metE 5-methyltetrahydropteroyltriglutamatehomocysteine S-methyltransferase 23.57 21.29...”
- “...and ABC transporter and permease protein, putative ( EF_2049 ), while glucan 1,6-alpha-glucosidase, putative ( EF_1348 ), Alpha-amylase ( EF_1349 ) and ABC transporter, permease protein ( EF_0180 ) were found to be more abundant in planktonic cell compared to in biofilm. Aside from the ABC...”
LBA1871 neopullulanase from Lactobacillus acidophilus NCFM
25% identity, 21% coverage
AAX84031.1 α-amylase (AmyF) (EC 3.2.1.1) (see protein)
25% identity, 30% coverage
SMU_2037 alpha,alpha-phosphotrehalase from Streptococcus mutans UA159
25% identity, 35% coverage
CAA02858.1 palatinase / trehalulose hydrolase (MutA) (EC 3.2.1.10|3.2.1.-) (see protein)
25% identity, 35% coverage
cg2529 trehalose synthase (maltose alpha-D-glucosyltransferase) from Corynebacterium glutamicum ATCC 13032
27% identity, 31% coverage
C1DMP8 isomaltulose synthase (EC 5.4.99.11) from Azotobacter vinelandii (see paper)
Avin_08330 sucrose isomerase from Azotobacter vinelandii AvOP
26% identity, 33% coverage
A0A1R4FYB1 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Corynebacterium glutamicum (see 2 papers)
27% identity, 30% coverage
Csac_0408 alpha amylase, catalytic region from Caldicellulosiruptor saccharolyticus DSM 8903
22% identity, 31% coverage
- Caldicellulosiruptor saccharolyticus transcriptomes reveal consequences of chemical pretreatment and genetic modification of lignocellulose
Blumer-Schuette, Microbial biotechnology 2017 - “...SigP TM AA CAZy Family Cellulose versus Xylan versus KGM P X CC KGM P Csac_0408 Amylase Y N 514 GH13 4.3 Csac_0678 Endoglucanase/endo1,4xylanase Y N 756 GH5, CBM28 12.8 11.4 8.8 Csac_0689 Pullulanase Y N 1136 CBM41, CBM48,GH13, CBM20 10.7 2.4 4.4 Csac_0696 Endo1,4xylanase Y...”
- “...are involved in glucan hydrolysis GH family 13, 15 and GT family 35 (Csac_0130, Csac_0203, Csac_0408, Csac_0426 and Csac_0429, see TableS7). In contrast, genes involved in the synthesis of glycogen were downregulated, supporting the assumption that the microbe is relying on energy stores for growth. Additionally,...”
- Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus
van, Applied and environmental microbiology 2008 - “...enzymes, such as an -amylase precursor (Csac_0408), an oligo-1,6-glucosidase (Csac_2428), a pullulanase (Csac_0689), a 4--glucanotransferase (Csac_0203), and a...”
- “...of the gene encoding the -amylase precursor mentioned above (Csac_0408), where it overlaps the putative MalR binding site, and once in the middle of the gene...”
MAC_00567 maltose permease from Metarhizium acridum
22% identity, 49% coverage
- MaAts, an Alkylsulfatase, Contributes to Fungal Tolerances against UV-B Irradiation and Heat-Shock in Metarhizium acridum
Song, Journal of fungi (Basel, Switzerland) 2022 - “...cell wall biosynthesis were down-regulated, such as phospholipase A gene (MAC_06774), a putative maltase gene (MAC_00567), a putative oxidoreductase gene (MAC_01213), and so on ( Table S3 ). It is worth noting that two DEGs, the beta-glucosidase gene (MAC_00623) and a putative C2H2 finger domain protein...”
- “...up-regulated in MaAts . In addition, ten cell wall-associated protein genes (MAC_06774 [ 63 ], MAC_00567 [ 64 ], MAC_01213 [ 65 ], MAC_01513 [ 66 ], MAC_02366 [ 67 ], MAC_02951 [ 68 ], MAC_04850 [ 69 ], MAC_06773 [ 70 ], MAC_00235 [ 71...”
Achr_33080 alpha-glucosidase from Azotobacter chroococcum NCIMB 8003
25% identity, 36% coverage
Tthe_1923 alpha-glucosidase from Thermoanaerobacterium thermosaccharolyticum DSM 571
23% identity, 35% coverage
BBB58_RS13090 glycoside hydrolase family 13 protein from Exiguobacterium profundum
33% identity, 15% coverage
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 789,361 different protein sequences to 1,256,019 scientific articles. Searches against EuropePMC were last performed on January 10 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory