PaperBLAST
PaperBLAST Hits for SwissProt::O25792 Nucleoside permease NupC (Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)) (418 a.a., MIFSSLFSVV...)
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>SwissProt::O25792 Nucleoside permease NupC (Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori))
MIFSSLFSVVGMAVLFLIAWVFSSNKRAINYRTIVSAFVIQVALGALALYVPLGREMLQG
LASGIQSVISYGYEGVRFLFGNLAPNAKGDQGIGGFVFAINVLAIIIFFASLISLLYYLK
IMPLFINLIGGALQKCLGTSRAESMSAAANIFVAHTEAPLVIKPYLKSMSDSEIFAVMCV
GMASVAGPVLAGYASMGIPLPYLIAASFMSAPGGLLFAKIIYPQNETISSHADVSIEKHV
NAIEAIANGASTGLNLALHVGAMLLAFVGMLALINGLLGVVGGFLGMEHLSLGLILGTLL
KPLAFMLGIPWSQAGIAGEIIGIKIALNEFVGYMQLLPYLGDNPPLILSEKTKAIITFAL
CGFANLSSVAMLIGGLGSLVPKKKDLIVRLALKAVLVGTLSNFMSATIAGLFIGLNAH
Running BLASTp...
Found 99 similar proteins in the literature:
nupC / O25792 purine nucleobase/nucleoside transporter from Helicobacter pylori (strain ATCC 700392 / 26695) (see 2 papers)
NUPC_HELPY / O25792 Nucleoside permease NupC from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
HP1180 pyrimidine nucleoside transport protein (nupC) from Helicobacter pylori 26695
100% identity, 100% coverage
- function: Involved in purine nucleosides uptake. Could also be involved in uptake of nucleobases.
disruption phenotype: Mutant is deficient for transport of inosine, guanosine or adenosine. - Delineation of the pH-Responsive Regulon Controlled by the Helicobacter pylori ArsRS Two-Component System
Loh, Infection and immunity 2021 (secret) - The Role of a Dipeptide Transporter in the Virulence of Human Pathogen, Helicobacter pylori
Xu, Frontiers in microbiology 2021 - “...gene 1.0557 HP1114 uvrB 1.6187 HP1162 Predicted gene 2.3026 HP1164 trxB 1.1085 HP1177 hopQ 1.657 HP1180 nupC 1.273 HP1181 Multidrug transporter 1.2283 HP1186 arsR 1.2884 HP1212 atpE 1.662 HP1283 Predicted gene 1.4378 HP1286 Predicted gene 1.6787 HP1288 Predicted gene 2.66 HP1289 Predicted gene 1.3261 HP1323 rnhB...”
- Efficiency of purine utilization by Helicobacter pylori: roles for adenosine deaminase and a NupC homolog
Miller, PloS one 2012 - “...Using mutant analysis we show that a strain lacking the gene encoding a NupC homolog (HP1180) was growth-retarded in a defined medium supplemented with certain purines. This strain was attenuated for uptake of radiolabeled adenosine, guanosine, and inosine, showing a role for this transporter in uptake...”
- “...is the mechanism by which purines are transported into the cell. The H. pylori protein HP1180 is homologous to the bacterial concentrative nucleoside transporter (CNT) NupC, a H + /nucleoside symporter. The best-studied bacterial NupC proteins (from Escherichia coli and Bacillus subtilis ) transport pyrimidine rather...”
- Characterization of the ArsRS regulon of Helicobacter pylori, involved in acid adaptation
Pflock, Journal of bacteriology 2006 - “...4.06 HP1129 JHP1057 2.81 HP1130 JHP1058 3.19 HP1174 HP1180 JHP1101 JHP1106 5.65 4.17 HP1175 JHP1102 2.12 HP0681 HP0682 HP0688* HP0689 HP0709 HP0947 HP1288*...”
- Expanded metabolic reconstruction of Helicobacter pylori (iIT341 GSM/GPR): an in silico genome-scale characterization of single- and double-deletion mutants
Thiele, Journal of bacteriology 2005 - “...HP1100 HP1108 to HP1111 HP1108 to HP1111 HP1118 HP1180 HP1257 HP1385 HP1399 HP1418 HP1491 HP1495 HP1505 pyrE fbp rocF murB tal ribD L-Asparagine, L-aspartate,...”
- “...PPM2) ......................................................................................................HP0574 (RPI) HP1180 (ADNt2, CYTDt2, DADNt2, DCYTt2,...”
- pH-regulated gene expression of the gastric pathogen Helicobacter pylori
Merrell, Infection and immunity 2003 - “...0.6 0.8 0.3 0.4 0.3 HP1562 JHP1470 0.7 0.3 0.3 0.3 HP1180 HP0653 HP0916 HP1512 HP0485 HP1193 JHP1106 JHP0598 0.4 0.8 0.7 0.5 1.1 0.7 0.2 0.5 0.3 0.3 0.4 0.4 0.3...”
- Helicobacter pylori physiology predicted from genomic comparison of two strains
Doig, Microbiology and molecular biology reviews : MMBR 1999 - “...it is likely that the putative transporter (JHP1106/ HP1180) is specific for nucleosides, which would allow H. pylori to obtain purines via the salvage...”
- Metabolism and genetics of Helicobacter pylori: the genome era
Marais, Microbiology and molecular biology reviews : MMBR 1999 - “...but no gene orthologous to upp was found. HP1180 has similarity to nupC, coding for a pyrimidine nucleoside transport protein in B. subtilis; however,...”
jhp1106 putative nucleoside transporter from Helicobacter pylori J99
96% identity, 100% coverage
NF38_02480 NupC/NupG family nucleoside CNT transporter from Haemophilus influenzae
HI0519 transport protein, putative from Haemophilus influenzae Rd KW20
57% identity, 99% coverage
- Inactivation of the Thymidylate Synthase thyA in Non-typeable Haemophilus influenzae Modulates Antibiotic Resistance and Has a Strong Impact on Its Interplay with the Host Airways
Rodríguez-Arce, Frontiers in cellular and infection microbiology 2017 - “...under TxS challenge (Chatterjee et al., 2008 ; Kriegeskorte et al., 2014 ). HI0519 and NF38_02480 are annotated as nupC in the RdKW20 and NTHi375 genomes, respectively (Fleischmann et al., 1995 ; Mell et al., 2014 ). Expression of the nupC gene in H. influenzae was...”
- Inactivation of the Thymidylate Synthase thyA in Non-typeable Haemophilus influenzae Modulates Antibiotic Resistance and Has a Strong Impact on Its Interplay with the Host Airways
Rodríguez-Arce, Frontiers in cellular and infection microbiology 2017 - “...for growth under TxS challenge (Chatterjee et al., 2008 ; Kriegeskorte et al., 2014 ). HI0519 and NF38_02480 are annotated as nupC in the RdKW20 and NTHi375 genomes, respectively (Fleischmann et al., 1995 ; Mell et al., 2014 ). Expression of the nupC gene in H....”
- Characterizations of highly expressed genes of four fast-growing bacteria
Karlin, Journal of bacteriology 2001 - “...and orf (E. coli yeiM and yeiJ, H. influenzae HI0519, and B. subtilis yutK) c The RNA helicase DeaD gene has multiple homologs in each genome, but...”
JYG29_07705 NupC/NupG family nucleoside CNT transporter from Vibrio alginolyticus
59% identity, 97% coverage
- Autographiviridae phage HH109 uses capsular polysaccharide for infection of Vibrio alginolyticus
Li, iScience 2024 - “...revealed that seven genes ( ugd , wecA , wzc , wzb , argE , JYG29_07705 , and JYG29_22310 ) were involved in transposon insertions in those eleven mutants, with two genes ( ugd and wecA ) represented in multiple mutants ( FigureS1 C). To further...”
- “...of the wild-type strain ( Figure1 ). However, deletions of JYG29_22310 , argE , and JYG29_07705 did not affect bacterial lysis caused by the phage HH109 infection ( FigureS1 D), which was inconsistent with the result of transposon insertion, indicating that polar effect occurred due to...”
VP2437 NupC family protein from Vibrio parahaemolyticus RIMD 2210633
58% identity, 97% coverage
TC 2.A.41.2.11 / Q9KPL5 Concentrative nucleoside transporter, CNT, of 418 aas and 12 TMSs. A repeat-swapped model of VcCNT predicts that nucleoside transport occurs via a mechanism involving an elevator-like substrate binding domain movement across the membrane from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
VC2352 NupC family protein from Vibrio cholerae O1 biovar eltor str. N16961
VC_2352 NupC/NupG family nucleoside CNT transporter from Vibrio cholerae O1 biovar El Tor str. N16961
56% identity, 98% coverage
- substrates: Nucleosides
- Cryo-EM structure of the human concentrative nucleoside transporter CNT3.
Zhou, PLoS biology 2020 - “...hCNT1 (UniProt no. O00337), hCNT2 (UniProt no. O43868), hCNT3 (UniProt no. Q9HAS3), vcCNT (UniProt no. Q9KPL5), and CNTnw (UniProt no. G4CRQ5). Sequences were aligned with ClustalW. Invariant and highly conserved residues are shaded yellow and gray, respectively. Residues responsible for substrate binding and sodium binding are...”
- A simple mechanism for integration of quorum sensing and cAMP signalling inV. cholerae
Walker, 2023 - A simple mechanism for integration of quorum sensing and cAMP signalling in Vibrio cholerae
Walker, eLife 2023 - “...1533703 (-) 1990133 VC1851 1990076.5 atattgagtaatcaattagtaa 1990031 (+) 2364721 (VC2212) 2364680.5 ctattaacagttttatttataa 2364774 (+) 2509878 VC2352 2509882.5 ttagtgacagatgcgtcattaa 2509790 (-) 2667349 VC2486 2667368.5 taattattaatttgaacaatag 2667206 (-) chromosome II 163808 VCA0148 163810.5 taattgattattgtgtaactat 163852 (-) 214589 (VCA0198) 214582.5 taattgataactttgacagtat 213494 (+) 237008 VCA0218<>VCA0219 R 237019.5 taaataatatgaatatcagtaa 237053...”
- “...in vivo, we observed repression in six cases in vitro ( hapR , VC0585 , VC2352 , VCA0219 , VCA0663, and VCA0960 ) (lanes 742). Conversely, the murQP promoter (P murQP ) subject to repression by HapR in vivo, generated no transcript in vitro (lanes 16)....”
- RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae
Peschek, Nature communications 2020 - “...nine targets ( crvA , irpA , rbmA , rbmD , vpsL , vpsU , vc2352 , vca0075 , and vca0864 ), while we were unable to validate direct regulation of bapI , rbmC , rbmF , rbsD , and vca0043 (Fig. 3a and Supplementary Fig....”
- “...and rbmD show only poor conservation (Supplementary Fig. 7 ). Additional targets of VadR are vc2352 (encoding a NupC-type nucleoside transporter 45 ), irpA (encoding an iron-regulated membrane protein carrying a peptidase domain 46 ), vca0864 (encoding a methyl-accepting chemotaxis protein 47 ), and vca0075 (function...”
- Transcriptomics reveals a cross-modulatory effect between riboflavin and iron and outlines responses to riboflavin biosynthesis and uptake in Vibrio cholerae
Sepúlveda-Cisternas, Scientific reports 2018 - “...ribD riboflavin-specific deaminase 1.385 VC2272 nrdR conserved hypothetical protein 1.858 VC2323 conserved hypothetical protein 1.227 VC2352 NupC family protein 1.381 1.164 VC2357 hypothetical protein 1.362 VC2361 grcA formate acetyl transferase-related protein 1.163 1.092 VC2363 thrB homoserine kinase 1.009 VC2364 thrA aspartokinase I/homoserine dehydrogenase threonine-sensitive 1.391 VC2367...”
- Nucleoside uptake in Vibrio cholerae and its role in the transition fitness from host to environment
Gumpenberger, Molecular microbiology 2016 (PubMed)- “...via identification and characterization of VCA0179, VC1953 and VC2352 representing the three nucleoside transport systems in V. cholerae. Based on our results...”
- “...crystal structure of the V. cholerae NupCfamily protein VC2352 has been solved (Johnson et al., 2012). Interestingly, in silico simulations and functional...”
- Vibrio cholerae phosphatases required for the utilization of nucleotides and extracellular DNA as phosphate sources
McDonough, Molecular microbiology 2016 - “...throughout the VC2174 coding region and two unique insertions were in the 5 end of VC2352 ( FigureS2 ). An in frame deletion of VC2174 in the wild type background results in white colonies on LB XP plates, validating the transposon screen results. However, a clean...”
- “...on LB XP plates, even when the VC2352 deletion was moved into the phoX strain. VC2352, encoding a NupChomolog, appears to be the dominant nucleoside transporter in V.cholerae [Gumpenberger etal . [accompanying manuscript from separate group)]. Our work suggests that expression of a truncated VC2352 somehow...”
- CytR Is a Global Positive Regulator of Competence, Type VI Secretion, and Chitinases in Vibrio cholerae
Watve, PloS one 2015 - “...annotated in V . cholerae as putative inner membrane nucleoside uptake transporter nupC , ( vc2352 ) [ 23 ]. The E . coli homolog of ycdZ , a putative inner membrane Nup protein [ 24 ] was also repressed, while the other two putative nupC...”
- Regulation of rugosity and biofilm formation in Vibrio cholerae: comparison of VpsT and VpsR regulons and epistasis analysis of vpsT, vpsR, and hapR
Beyhan, Journal of bacteriology 2007 - “...of three genes encoding NupC family proteins (VC1953, VC2352, and VCA0179) that are predicted to be involved in nucleoside transport was higher in the...”
- The Structure and Mechanism of Drug Transporters
Roberts, Methods in molecular biology (Clifton, N.J.) 2021 - “...5A9H 2.06 - EC domain ( 207 ) X 5A9I 2.84 - EC domain SLC28 VC_2352 vcCNT V. cholerae X 3TIJ 2..44 I with uridine and Na + ( 167 ) VC_2352 vcCNT V. cholerae X 4PDA 2.61 I with cytidine and Na + ( 227...”
4pb1A / Q9KPL5 Structure of vccnt-7c8c bound to ribavirin (see paper)
56% identity, 98% coverage
- Ligand: 1-(beta-d-ribofuranosyl)-1h-1,2,4-triazole-3-carboxamide (4pb1A)
WP_009116906 NupC/NupG family nucleoside CNT transporter from Neisseria wadsworthii
G4CRQ5 Nucleoside permease from Neisseria wadsworthii 9715
57% identity, 97% coverage
- Visualizing multistep elevator-like transitions of a nucleoside transporter
Hirschi, Nature 2017 - “...CNT NW can be found in the National Center for Biotechnology Information under accession code WP_009116906. Atomic coordinates and structure factors for the reported crystal structures are deposited in the Protein Data Bank under accession codes 5L26 (substrate-bound inward-facing, CNT NW ), 5L27 (intermediate-1, CNT NW...”
- Cryo-EM structure of the human concentrative nucleoside transporter CNT3.
Zhou, PLoS biology 2020 - “...(UniProt no. O43868), hCNT3 (UniProt no. Q9HAS3), vcCNT (UniProt no. Q9KPL5), and CNTnw (UniProt no. G4CRQ5). Sequences were aligned with ClustalW. Invariant and highly conserved residues are shaded yellow and gray, respectively. Residues responsible for substrate binding and sodium binding are indicated by blue circles, purple...”
U876_03055 NupC/NupG family nucleoside CNT transporter from Aeromonas hydrophila NJ-35
59% identity, 96% coverage
5l2aA / G4CRQ5 Structure of cntnw n149s,f366a in an outward-facing state (see paper)
57% identity, 97% coverage
- Ligand: 2-{[(4-o-alpha-d-glucopyranosyl-beta-d-glucopyranosyl)oxy]methyl}-2-octyldecyl 4-o-alpha-d-glucopyranosyl-beta-d-glucopyranoside (5l2aA)
WP_004146034 NupC/NupG family nucleoside CNT transporter from Klebsiella pneumoniae
54% identity, 96% coverage
- Elucidation of the Gemcitabine Transporters of Escherichia coli K-12 and Gamma-Proteobacteria Linked to Gemcitabine-Related Chemoresistance
Iosifidou, International journal of molecular sciences 2024 - “...and YegT (P76417) (UniProt numbers given in parentheses), K. pneumoniae KpNupC (A0A2W0KM59), KpNupC2 (WP_002898911), KpvcCNT (WP_004146034), and KpNupG (WP_038806797), and C. freundii CfNupC (A0A336NW46), CfPsuT (D2TRJ2), CfNupG (A0A7D6VR53), CfXapB (A0A7W3D7V4), and CfYegT (A0A7D6VSQ9) (trEMBL or NCBI accession numbers in parentheses). Other sequences of NHS homologs shown...”
YPTB0580 putative Na+ dependent nucleoside transporter-family protein from Yersinia pseudotuberculosis IP 32953
YPK_3628 nucleoside transporter from Yersinia pseudotuberculosis YPIII
57% identity, 97% coverage
- Yersinia pseudotuberculosis BarA-UvrY Two-Component Regulatory System Represses Biofilms via CsrB
Schachterle, Frontiers in cellular and infection microbiology 2018 - “...hldD ADP-L-glycero-D-manno-heptose-6-epimerase 233986 YPTB0194 uvrD helicase 420511 YPTB0355 Putative phage inhibition, colistin resistance protein 683674 YPTB0580 Putative Na+ dependent nucleoside transporter-family protein 702455 YPTB0594 Putative Ca++ transporting P-type ATPase 729594 YPTB0614 nhaR transcriptional activator protein 757451 intergenic, adjacent to djlA colanic acid regulator 903733 YPTB0750 barA...”
- Functional versatility of Zur in metal homeostasis, motility, biofilm formation, and stress resistance in Yersinia pseudotuberculosis
Gu, Microbiology spectrum 2024 - “...and YPK_0382), simple sugar (YPK_24082411), glutamate/aspartate (YPK_3010), oligopeptide (YPK_2067 and YPK_2068), and nucleoside (YPK_1438 and YPK_3628) transport systems were upregulated by Zur. This suggests Zurs role in nutrient acquisition in Y. pseudotuberculosis . Although similar regulation of amino acid and oligopeptide transport systems was revealed, Zur...”
Shewana3_1039 nucleoside permease (cytidine, inosine, uridine, or thymidine) from Shewanella sp. ANA-3
53% identity, 98% coverage
- mutant phenotype: Specifically important for utilization of cytidine, inosine, uridine, and thymidine.
SO3706, SO_3706 NupC family protein from Shewanella oneidensis MR-1
51% identity, 97% coverage
VC1953 NupC family protein from Vibrio cholerae O1 biovar eltor str. N16961
52% identity, 98% coverage
- A dual-function RNA balances carbon uptake and central metabolism in Vibrio cholerae
Venkat, The EMBO journal 2021 - “..., treB , nagE , ptsH , ptsI for VcdR and vca0053 , vc2761 , vc1953 , vc1449 , lamB for VcdP) in wildtype, vcdRP , and VcdRP overexpression cells (Fig EV2C ). In all cases, we observed that genes differentially controlled by VcdR or VcdP...”
- Nucleoside uptake in Vibrio cholerae and its role in the transition fitness from host to environment
Gumpenberger, Molecular microbiology 2016 (PubMed)- “...uptake via identification and characterization of VCA0179, VC1953 and VC2352 representing the three nucleoside transport systems in V. cholerae. Based on...”
- “...and characterized the three NupC family proteins VCA0179, VC1953 and VC2352 to be required for NS transport. Our data indicate that all three NupC-homologs...”
- CytR Is a Global Positive Regulator of Competence, Type VI Secretion, and Chitinases in Vibrio cholerae
Watve, PloS one 2015 - “...24 ] was also repressed, while the other two putative nupC homologs ( vca0179 and vc1953 ) were not. Gumpenberger and coworkers have recently reported that all three nupC homologs are repressed by CytR in V . cholerae [ 25 ]. However, these experiments were performed...”
- Regulation of rugosity and biofilm formation in Vibrio cholerae: comparison of VpsT and VpsR regulons and epistasis analysis of vpsT, vpsR, and hapR
Beyhan, Journal of bacteriology 2007 - “...expression of three genes encoding NupC family proteins (VC1953, VC2352, and VCA0179) that are predicted to be involved in nucleoside transport was higher in...”
SO1214 NupC family protein from Shewanella oneidensis MR-1
51% identity, 95% coverage
VDA_001789 NupC/NupG family nucleoside CNT transporter from Photobacterium damselae subsp. damselae CIP 102761
50% identity, 98% coverage
- Exposure of the Opportunistic Marine Pathogen Photobacterium damselae subsp. damselae to Human Body Temperature Is a Stressful Condition That Shapes the Transcriptome, Viability, Cell Morphology, and Virulence
Matanza, Frontiers in microbiology 2020 - “...MotB 4.1 9.0774E129 ChrI VDA_002607 Flagellin protein FlaB 4.5 2.4193E132 ChrI Porins, permeases, and transporters VDA_001789 Nucleoside permease NupC 10.7 0 ChrI VDA_001677 Sulfate permease 6.5 0 ChrI VDA_002944 Probable low-affinity inorganic phosphate transporter 6.8 0 ChrI VDA_002943 Phosphate transport regulator 7.4 0 ChrI Transcriptional regulators...”
- “...with E. coli pitA (identity 33%), are down-regulated at 37C. As well, nucleoside permeases NupC (VDA_001789 and VDA_002313) were also down-regulated at 37C. A previous study ( Matanza and Osorio, 2018 ) showed that NupC VDA_001789 presented a high up-regulation at 25C compared to 15C. These...”
- Transcriptome changes in response to temperature in the fish pathogen Photobacterium damselae subsp. damselae: Clues to understand the emergence of disease outbreaks at increased seawater temperatures
Matanza, PloS one 2018 - “...the annotation in the RM-71 genome. Gene ID Product/Function Fold Change p-value Location Nutrient acquisition/metabolism VDA_001789 Nucleoside permease NupC 5.1 2.2182E-122 ChrI VDA_001833 GPR1/FUN34/yaaH putative acetate transporter 3.6 5.07132E-17 ChrI VDA_002532 Porin 5.1 1.08929E-77 ChrI VDA_003254 Porin 3.0 3.53973E-49 ChrI VDA_001005 Porin 2.5 3.2873E-69 ChrII VDA_003133...”
- “...transporters and porins were upregulated at 25C ( Table 1 ). The nucleoside permease NupC (VDA_001789) was the most upregulated gene at 25C. A V . cholerae nupC deletion mutant was impaired for nucleoside acquisition leading to diminished fitness in nutrient-limited environments [ 44 ]. Growth...”
QR722_RS15920 NupC/NupG family nucleoside CNT transporter from Aliiglaciecola sp. LCG003
51% identity, 98% coverage
VFMJ11_1398 NupC/NupG family nucleoside CNT transporter from Aliivibrio fischeri MJ11
VFMJ11_1398 Na+ dependent nucleoside transporter family protein from Vibrio fischeri MJ11
51% identity, 98% coverage
A0J47_RS19570 NupC/NupG family nucleoside CNT transporter from Photobacterium damselae subsp. damselae
47% identity, 98% coverage
AR442_06895 NupC/NupG family nucleoside CNT transporter from Bacillus velezensis
46% identity, 98% coverage
CG479_RS18930 NupC/NupG family nucleoside CNT transporter from Bacillus cytotoxicus
49% identity, 94% coverage
O32115 Uncharacterized transporter YutK from Bacillus subtilis (strain 168)
BSU32180 putative Na+(H+)/nucleoside cotransporter from Bacillus subtilis subsp. subtilis str. 168
47% identity, 98% coverage
VCA0179 NupC family protein from Vibrio cholerae O1 biovar eltor str. N16961
45% identity, 98% coverage
- Nucleoside uptake in Vibrio cholerae and its role in the transition fitness from host to environment
Gumpenberger, Molecular microbiology 2016 (PubMed)- “...nucleoside uptake via identification and characterization of VCA0179, VC1953 and VC2352 representing the three nucleoside transport systems in V. cholerae....”
- “...V. cholerae and characterized the three NupC family proteins VCA0179, VC1953 and VC2352 to be required for NS transport. Our data indicate that all three...”
- CytR Is a Global Positive Regulator of Competence, Type VI Secretion, and Chitinases in Vibrio cholerae
Watve, PloS one 2015 - “...protein [ 24 ] was also repressed, while the other two putative nupC homologs ( vca0179 and vc1953 ) were not. Gumpenberger and coworkers have recently reported that all three nupC homologs are repressed by CytR in V . cholerae [ 25 ]. However, these experiments...”
- Mining regulatory 5'UTRs from cDNA deep sequencing datasets
Livny, Nucleic acids research 2010 - “...17 VCA0142 C4-dicarboxylate transport transcriptional regulatory protein MOCO RNA motif(1), GEMM RNA motif(2) RLE0123(2) 18 VCA0179 NupC family protein Purine(11) 19 VCA0278 Serine hydroxymethyltransferase Glycine(8) RLE0085(7) 20 VCA0287 Threonyl-tRNA synthetase T-box(60) RLE020 (5) C1 * VC2490 2-isopropylmalate synthase T-box(11), Leu leader(28), ydaO-yuaA(1) 21 VC0007 50S ribosomal...”
- Regulation of rugosity and biofilm formation in Vibrio cholerae: comparison of VpsT and VpsR regulons and epistasis analysis of vpsT, vpsR, and hapR
Beyhan, Journal of bacteriology 2007 - “...genes encoding NupC family proteins (VC1953, VC2352, and VCA0179) that are predicted to be involved in nucleoside transport was higher in the RvpsT mutant....”
BCAS0730 putative Na+ dependent nucleoside transporter family protein from Burkholderia cenocepacia J2315
46% identity, 93% coverage
YP_001672959 Na+ dependent nucleoside transporter domain protein from Shewanella halifaxensis HAW-EB4
49% identity, 99% coverage
SO4296 NupC family protein from Shewanella oneidensis MR-1
47% identity, 97% coverage
BMB171_RS26110 NupC/NupG family nucleoside CNT transporter from Bacillus thuringiensis BMB171
46% identity, 94% coverage
- NupR Responding to Multiple Signals Is a Nucleoside Permease Regulator in Bacillus thuringiensis BMB171
Qin, Microbiology spectrum 2022 - “...Nucleoside transporter NupC family 5.358 AATGGTCAGACGTCATTCGT Pro BMB171_RS25250 Nucleoside transporter NupC family 5.034 AATGGTCAGACGTTCAACGT Pro BMB171_RS26110 Nucleoside transporter NupC family 5.397 GGAGGTCAAACGTCTAATGA Pro BMB171_RS26735 Bacillolysin 1.452 AGTGGTATGACAACTCAAAA Pro BMB171_RS26950 HAD superfamily hydrolase 1.707 AATAGTAAGACATCAAACCT Pro BMB171_RS18795 GntR family transcriptional regulator CGACCTCTGATGTAAGACTT Pro BMB171_RS26765 Collagen adhesion protein...”
NupX / b2161 putative nucleoside transporter from Escherichia coli K-12 substr. MG1655 (see 2 papers)
nupX / P33021 putative nucleoside transporter from Escherichia coli (strain K12) (see paper)
NUPX_ECOLI / P33021 Putative nucleoside permease NupX from Escherichia coli (strain K12) (see paper)
TC 2.A.41.2.10 / P33021 Nucleoside permease NupX from Escherichia coli (strain K12) (see 4 papers)
44% identity, 98% coverage
- substrates: Nucleosides
- Elucidation of the Gemcitabine Transporters of Escherichia coli K-12 and Gamma-Proteobacteria Linked to Gemcitabine-Related Chemoresistance
Iosifidou, International journal of molecular sciences 2024 - “...sequences. The genes mobilized correspond to the coding sequences of E. coli NupC (P0AFF2), NupX (P33021), YeiM/PsuT (P33024), NupG (P0AFF4), XapB (P45562), and YegT (P76417) (UniProt numbers given in parentheses), K. pneumoniae KpNupC (A0A2W0KM59), KpNupC2 (WP_002898911), KpvcCNT (WP_004146034), and KpNupG (WP_038806797), and C. freundii CfNupC (A0A336NW46),...”
S2375 putative transport system permease protein from Shigella flexneri 2a str. 2457T
44% identity, 98% coverage
ECs3053 putative transport system permease protein from Escherichia coli O157:H7 str. Sakai
44% identity, 98% coverage
D2TRJ2 Nucleoside permease from Citrobacter rodentium (strain ICC168)
44% identity, 98% coverage
- Elucidation of the Gemcitabine Transporters of Escherichia coli K-12 and Gamma-Proteobacteria Linked to Gemcitabine-Related Chemoresistance
Iosifidou, International journal of molecular sciences 2024 - “...KpNupC (A0A2W0KM59), KpNupC2 (WP_002898911), KpvcCNT (WP_004146034), and KpNupG (WP_038806797), and C. freundii CfNupC (A0A336NW46), CfPsuT (D2TRJ2), CfNupG (A0A7D6VR53), CfXapB (A0A7W3D7V4), and CfYegT (A0A7D6VSQ9) (trEMBL or NCBI accession numbers in parentheses). Other sequences of NHS homologs shown in Figure 3 A are Cf-Yeg-x (A0A0D7M2I3), Cf-Yeg-1 (A0A7W3HTK5), and...”
PsuT / b2164 putative pseudouridine transporter from Escherichia coli K-12 substr. MG1655 (see 4 papers)
P33024 Putative pseudouridine transporter from Escherichia coli (strain K12)
45% identity, 98% coverage
- Elucidation of the Gemcitabine Transporters of Escherichia coli K-12 and Gamma-Proteobacteria Linked to Gemcitabine-Related Chemoresistance
Iosifidou, International journal of molecular sciences 2024 - “...genes mobilized correspond to the coding sequences of E. coli NupC (P0AFF2), NupX (P33021), YeiM/PsuT (P33024), NupG (P0AFF4), XapB (P45562), and YegT (P76417) (UniProt numbers given in parentheses), K. pneumoniae KpNupC (A0A2W0KM59), KpNupC2 (WP_002898911), KpvcCNT (WP_004146034), and KpNupG (WP_038806797), and C. freundii CfNupC (A0A336NW46), CfPsuT (D2TRJ2),...”
S2378 putative transport system permease protein from Shigella flexneri 2a str. 2457T
45% identity, 98% coverage
SSKA14_2274 NupC/NupG family nucleoside CNT transporter from Stenotrophomonas maltophilia
40% identity, 94% coverage
NP_113852 sodium/nucleoside cotransporter 2 from Rattus norvegicus
36% identity, 62% coverage
- Localization of nucleoside transporters in rat epididymis.
Klein, Journal of biochemical and molecular toxicology 2017 (PubMed)- GeneRIF: The purpose of this study is to determine the localization of nucleoside transporters expressed within rat epididymis to demonstrate the potential of epididymal reabsorption. Using immunohistochemistry, we determined that equilibrative nucleoside transporter 1 (ENT1) is localized to the basolateral membrane of epithelial cells, ENT2 is expressed in the nucleus of the epithelium and CNT2 is expressed by basal cells.
- rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane.
Pinilla-Macua, FEBS letters 2014 (PubMed)- GeneRIF: rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane
- Hypoxia and P1 receptor activation regulate the high-affinity concentrative adenosine transporter CNT2 in differentiated neuronal PC12 cells.
Medina-Pulido, The Biochemical journal 2013 (PubMed)- GeneRIF: CNT2 is likely to modulate extracellular adenosine and cell energy balance in neuronal tissue.
- Structural determinants for rCNT2 sorting to the plasma membrane of polarized and non-polarized cells.
Pinilla-Macua, The Biochemical journal 2012 (PubMed)- GeneRIF: the N-terminal tail of rCNT2 contains dual sorting signals. An acidic region is responsible for its proper stabilization at the plasma membrane, whereas the putative CK2 domain (Ser(46)) is implicated in the apical sorting of the transporter.
- Mechanism of nucleoside uptake in rat placenta and induction of placental CNT2 in experimental diabetes.
Nishimura, Drug metabolism and pharmacokinetics 2012 (PubMed)- GeneRIF: Report induction of CNT2 in experimental diabetes mellitus.
- Striking species difference in the contribution of concentrative nucleoside transporter 2 to nucleoside uptake between mouse and rat hepatocytes.
Furihata, Antimicrobial agents and chemotherapy 2010 - GeneRIF: Data concluded that CNT2 contributes considerably to nucleoside uptake in rat hepatocytes but not in mouse hepatocytes.
- Bile acids alter the subcellular localization of CNT2 (concentrative nucleoside cotransporter) and increase CNT2-related transport activity in liver parenchymal cells.
Fernández-Veledo, The Biochemical journal 2006 - GeneRIF: Bile acids were found to increase CNT2-related transport activity in a K(ATP) channel-independent manner in both Fao hepatoma and rat liver parenchymal cells.
- Characterization of the rat Na+/nucleoside cotransporter 2 and transport of nucleoside-derived drugs using electrophysiological methods.
Larráyoz, American journal of physiology. Cell physiology 2006 (PubMed)- GeneRIF: two-electrode voltage-clamp technique was used to investigate CNT2 transport mechanism and study the interaction of nucleoside-derived drugs with the transporter expressed in Xenopus laevis oocytes
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S28A2_RAT / Q62773 Sodium/nucleoside cotransporter 2; Concentrative nucleoside transporter 2; CNT 2; rCNT2; Na(+)/nucleoside cotransporter 2; Sodium-coupled nucleoside transporter 2; Sodium/purine nucleoside cotransporter; SPNT; Solute carrier family 28 member 2 from Rattus norvegicus (Rat) (see 3 papers)
TC 2.A.41.2.1 / Q62773 Purine-specific nucleoside:Na+ symporter (see 3 papers)
36% identity, 62% coverage
- function: Sodium-dependent and purine-selective (PubMed:1315767, PubMed:7775409, PubMed:8967974). Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine) (PubMed:1315767, PubMed:7775409, PubMed:8967974). Accepts purine, analogs of purine nucleosides and uridine, and exhibits high affinity for adenosine (PubMed:7775409). May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (By similarity).
catalytic activity: adenosine(out) + Na(+)(out) = adenosine(in) + Na(+)(in) (RHEA:69927)
catalytic activity: inosine(out) + Na(+)(out) = inosine(in) + Na(+)(in) (RHEA:69931)
catalytic activity: guanosine(out) + Na(+)(out) = guanosine(in) + Na(+)(in) (RHEA:69935)
catalytic activity: uridine(out) + Na(+)(out) = uridine(in) + Na(+)(in) (RHEA:69887) - substrates: Na+, Purines
O88627 Sodium/nucleoside cotransporter 2 from Mus musculus
36% identity, 62% coverage
- Unraveling the Proteomic Landscape of Intestinal Epithelial Cell-Derived Exosomes in Mice.
Ding, Frontiers in physiology 2022 - “...(ileum/jejunum) P -value Expression 1 Q8R0I0 Ace2 Angiotensin-converting enzyme 2 5441.54 0.685 0.008 Down 2 O88627 Slc28a2 Sodium/nucleoside cotransporter 2 93.84 0.680 0.014 Down 3 Q3U9N9 Slc16a10 Monocarboxylate transporter 10 167.24 0.668 0.019 Down 4 Q0VG18 Smim24 Small integral membrane protein 24 682.87 0.668 0.002 Down...”
- “...Down 22 Q9EPR4 Slc23a2 Solute carrier family 23 member 2 53.34 0.685 0.045 Down 23 O88627 Slc28a2 Sodium/nucleoside cotransporter 2 93.84 0.680 0.014 Down 24 Q3U9N9 Slc16a10 Monocarboxylate transporter 10 167.24 0.668 0.019 Down 25 Q9QXW9 Slc7a8 Large neutral amino acids transporter small subunit 2 92.10...”
- iTRAQ-Based Quantitative Proteomic Analysis of Intestines in Murine Polymicrobial Sepsis with Hydrogen Gas Treatment.
Jiang, Drug design, development and therapy 2020 - “...Synapse-associated protein 1 Q9D5V6 Syap1 2.4945 0.817 Lithostathine-2 Q08731 Reg2 2.0655 1.2855 Sodium/nucleoside cotransporter 2 O88627 Slc28a2 2.009 1.2005 CCAAT/enhancer-binding protein beta P28033 Cebpb 1.8885 1.216 Protein DPCD Q8BPA8 Dpcd 1.8235 1.0625 Alpha-2-antiplasmin Q61247 Serpinf2 1.8155 0.9275 Ribosome biogenesis protein BOP1 P97452 Bop1 1.7595 1.204 Insulin...”
Q7UKU0 Broadly selective sodium/nucleoside transporter hfCNT from Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)
39% identity, 95% coverage
TC 2.A.41.2.5 / Q9UA35 Broadly selective nucleoside:Na+ cotransporter, hfCNT (transports uridine, thymidine, inosine, 3'-azido-3'deoxythymidine, 2'3'dideoxycytidine, and 2'3'dideoxyinosine) (Na+/uridine = 2) from Eptatretus stoutii (Pacific hagfish) (see paper)
35% identity, 59% coverage
S28A1_RAT / Q62674 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Rattus norvegicus (Rat) (see 3 papers)
TC 2.A.41.2.2 / Q62674 Nonspecific nucleoside:Na+ symporter from Rattus norvegicus (Rat) (see paper)
Slc28a1 / RF|NP_446315.1 sodium/nucleoside cotransporter 1 from Rattus norvegicus
36% identity, 63% coverage
- function: Sodium and pyrimidine nucleoside symporter of the plasma membrane that imports uridine, thymidine and cytidine into cells by coupling their transport to the transmembrane sodium electrochemical gradient. Also transports adenosine, an atypical substrate transported with high apparent affinity, but low maximum velocity. Therefore, exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (PubMed:8027026, PubMed:8967974). Involved in renal nucleoside (re)absorption (By similarity).
catalytic activity: uridine(out) + Na(+)(out) = uridine(in) + Na(+)(in) (RHEA:69887)
catalytic activity: thymidine(out) + Na(+)(out) = thymidine(in) + Na(+)(in) (RHEA:69891)
catalytic activity: cytidine(out) + Na(+)(out) = cytidine(in) + Na(+)(in) (RHEA:69895)
catalytic activity: adenosine(out) + Na(+)(out) = adenosine(in) + Na(+)(in) (RHEA:69927) - substrates: Na+, Nucleosides
S28A2_HUMAN / O43868 Sodium/nucleoside cotransporter 2; Concentrative nucleoside transporter 2; CNT 2; hCNT2; Na(+)/nucleoside cotransporter 2; Sodium-coupled nucleoside transporter 2; Sodium/purine nucleoside co-transporter; SPNT; Solute carrier family 28 member 2 from Homo sapiens (Human) (see 4 papers)
TC 2.A.41.2.4 / O43868 Purine nucleoside, uridine, and 2'3'dideoxyinosine cladribrine:Na+ symporter, CNT2 (Na+/nucleoside = 1) from Homo sapiens (Human) (see 7 papers)
NP_004203 sodium/nucleoside cotransporter 2 from Homo sapiens
35% identity, 61% coverage
- function: Sodium-dependent and purine-selective transporter (PubMed:10087507, PubMed:9435697). Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine) (PubMed:10087507, PubMed:21795683, PubMed:9435697). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues (PubMed:9435697). May contribute to regulate the transport of organic compounds in testes across the blood-testis- barrier (Probable).
catalytic activity: adenosine(out) + Na(+)(out) = adenosine(in) + Na(+)(in) (RHEA:69927)
catalytic activity: inosine(out) + Na(+)(out) = inosine(in) + Na(+)(in) (RHEA:69931)
catalytic activity: guanosine(out) + Na(+)(out) = guanosine(in) + Na(+)(in) (RHEA:69935)
catalytic activity: uridine(out) + Na(+)(out) = uridine(in) + Na(+)(in) (RHEA:69887) - substrates: 2'3'dideoxyinosine cladribrine, Na+, Purines, Uridine
- Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters.
Yamamura, Biochemical and biophysical research communications 2021 (PubMed)- GeneRIF: Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters.
- Common variants in the SLC28A2 gene are associated with serum uric acid level and hyperuricemia and gout in Han Chinese.
Zhou, Hereditas 2019 - GeneRIF: significant association of intron variants in the SLC28A2 gene with both serum uric acid level and hyperuricemia phenotype, and another exonic variant with gout in Han Chinese
- Substituted cysteine accessibility method (SCAM) analysis of the transport domain of human concentrative nucleoside transporter 3 (hCNT3) and other family members reveals features of structural and functional importance.
Mulinta, The Journal of biological chemistry 2017 - GeneRIF: results that validate the newly developed structural homology model of CNT membrane architecture for human CNTs, revealed extended conformationally mobile regions within transport-domain TMs, identified pore-lining residues of functional importance, and provided evidence of an emerging novel elevator-type mechanism of transporter function.
- Impact of genetic SLC28 transporter and ITPA variants on ribavirin serum level, hemoglobin drop and therapeutic response in patients with HCV infection.
Rau, Journal of hepatology 2013 (PubMed)- GeneRIF: association between ribavirin (RBV) serum levels and SLC28A2 rs11854484 genotype, as well as the replicated association of ITPA and SLC28A3 genetic polymorphisms with RBV-induced anemia and treatment response
- Negative predictive value of IL28B, SLC28A2, and CYP27B1 SNPs and low RBV plasma exposure for therapeutic response to PEG/IFN-RBV treatment.
D'Avolio, Therapeutic drug monitoring 2012 (PubMed)- GeneRIF: genetic association studies in population in Italy: Data suggest that 3 SNPs (SLC28A2 rs11854484; IL28B, rs8099917; CYP27B1, rs4646536) are associated with pharmacokinetics of ribavirin and thus, sustained virologic response in hepatitis C patients.
- Single nucleotide polymorphisms of gemcitabine metabolic genes and pancreatic cancer survival and drug toxicity.
Okazaki, Clinical cancer research : an official journal of the American Association for Cancer Research 2010 - GeneRIF: Clinical trial of gene-disease association, gene-gene interaction, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
- Contribution of adenosine related genes to the risk of depression with disturbed sleep.
Gass, Journal of affective disorders 2010 (PubMed)- GeneRIF: Observational study of gene-disease association. (HuGE Navigator)
- Link between high-affinity adenosine concentrative nucleoside transporter-2 (CNT2) and energy metabolism in intestinal and liver parenchymal cells.
Huber-Ruano, Journal of cellular physiology 2010 (PubMed)- GeneRIF: These findings support the theory that CNT2 plays roles other than salvage and establishes links with energy metabolism.
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- Changes in inflammation and oxidative stress signalling pathways in coarcted aorta triggered by bicuspid aortic valve and growth in young children
Skeffington, Experimental and therapeutic medicine 2020 - “...0.60 1.71 P07451 CA3 Carbonic anhydrase 3 1.47 3.78 0.45 0.73 0.39 0.028 -1.37 1.55 O43868 SLC28A2 Sodium/nucleoside cotransporter 2 5.72 18.47 1.44 6.25 0.31 0.042 -1.69 1.38 Table II Phosphoproteins differentially (fold increase >1.3 or <0.769) and significantly (P<0.05) expressed in coarcted aorta from paediatric...”
- Cryo-EM structure of the human concentrative nucleoside transporter CNT3.
Zhou, PLoS biology 2020 - “...additional data file. S6 Fig Sequence alignment of hCNT1 (UniProt no. O00337), hCNT2 (UniProt no. O43868), hCNT3 (UniProt no. Q9HAS3), vcCNT (UniProt no. Q9KPL5), and CNTnw (UniProt no. G4CRQ5). Sequences were aligned with ClustalW. Invariant and highly conserved residues are shaded yellow and gray, respectively. Residues...”
- THE CONCISE GUIDE TO PHARMACOLOGY 2017/18: Transporters
Alexander, British journal of pharmacology 2017 - “...28 member 3 Systematic nomenclature SLC28A1 SLC28A2 SLC28A3 HGNC, UniProt SLC28A1 , O00337 SLC28A2 , O43868 SLC28A3 , Q9HAS3 Common abreviation CNT1 CNT2 CNT3 Substrates ribavirin [ 98 ], gemcitabine [ 97 ], zalcitabine , zidovudine cladribine [ 416 ], didanosine , vidarabine , fludarabine [...”
- The Concise Guide to PHARMACOLOGY 2015/16: Transporters
Alexander, British journal of pharmacology 2015 - “...SLC28A1 SLC28A2 SLC28A3 Common abreviation CNT1 CNT2 CNT3 HGNC, UniProt SLC28A1 , O00337 SLC28A2 , O43868 SLC28A3 , Q9HAS3 Substrates gemcitabine [ 90 ], zalcitabine , zidovudine cladribine [ 376 ], didanosine , vidarabine , fludarabine [ 298 ], formycin B [ 298 ] zalcitabine ,...”
- The Concise Guide to PHARMACOLOGY 2013/14: transporters
Alexander, British journal of pharmacology 2013 - “...Systematic nomenclature SLC28A1 SLC28A2 SLC28A3 Common abbreviation CNT1 CNT2 CNT3 HGNC, UniProt SLC28A1, O00337 SLC28A2, O43868 SLC28A3, Q9HAS3 Endogenous substrates adenosine, uridine, thymidine, cytidine adenosine, inosine, guanosine, thymidine adenosine, inosine, uridine, guanosine, thymidine, cytidine Substrates gemcitabine, zidovudine, zalcitabine formycin B, cladribine, fludarabine, vidarabine, didanosine 5-fluorouridine, zebularine,...”
S28A1_RABIT / Q9MZT2 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Oryctolagus cuniculus (Rabbit) (see paper)
35% identity, 62% coverage
- function: Sodium and pyrimidine nucleoside symporter of the plasma membrane that imports uridine, thymidine and cytidine into cells by coupling their transport to the transmembrane sodium electrochemical gradient (PubMed:11028933). Also transports adenosine, an atypical substrate transported with high apparent affinity, but low maximum velocity. Therefore, exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit). Involved in renal nucleoside (re)absorption (By similarity).
catalytic activity: uridine(out) + Na(+)(out) = uridine(in) + Na(+)(in) (RHEA:69887)
catalytic activity: thymidine(out) + Na(+)(out) = thymidine(in) + Na(+)(in) (RHEA:69891)
catalytic activity: cytidine(out) + Na(+)(out) = cytidine(in) + Na(+)(in) (RHEA:69895)
catalytic activity: adenosine(out) + Na(+)(out) = adenosine(in) + Na(+)(in) (RHEA:69927)
XP_002690952 sodium/nucleoside cotransporter 2 from Bos taurus
34% identity, 62% coverage
S28A1_PIG / O62667 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Sus scrofa (Pig) (see paper)
35% identity, 63% coverage
- function: Sodium and pyrimidine nucleoside symporter of the plasma membrane that imports uridine, thymidine and cytidine into cells by coupling their transport to the transmembrane sodium electrochemical gradient. Also transports adenosine, an atypical substrate transported with high apparent affinity, but low maximum velocity. Therefore, exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit). Involved in renal nucleoside (re)absorption.
catalytic activity: uridine(out) + Na(+)(out) = uridine(in) + Na(+)(in) (RHEA:69887)
catalytic activity: thymidine(out) + Na(+)(out) = thymidine(in) + Na(+)(in) (RHEA:69891)
catalytic activity: cytidine(out) + Na(+)(out) = cytidine(in) + Na(+)(in) (RHEA:69895)
catalytic activity: adenosine(out) + Na(+)(out) = adenosine(in) + Na(+)(in) (RHEA:69927)
XP_011520510 sodium/nucleoside cotransporter 1 isoform X2 from Homo sapiens
35% identity, 60% coverage
- Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters.
Yamamura, Biochemical and biophysical research communications 2021 (PubMed)- GeneRIF: Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters.
- Expression of the nucleoside transporters hENT1 (SLC29) and hCNT1 (SLC28) in pediatric acute myeloid leukemia.
Jaramillo, Nucleosides, nucleotides & nucleic acids 2020 (PubMed)- GeneRIF: Expression of the nucleoside transporters hENT1 (SLC29) and hCNT1 (SLC28) in pediatric acute myeloid leukemia.
- Genome-Wide Analysis in Brazilians Reveals Highly Differentiated Native American Genome Regions.
Mychaleckyj, Molecular biology and evolution 2017 - GeneRIF: Brazilian Amerindian ancestry compared to Asian, European, and African Genomes.SNPs within or proximal to CIITA (rs6498115), SMC6 (rs1834619), and KLHL29 (rs2288697) were most differentiated in the Amerindian-specific branch. SNPs in ADAMTS9 (rs7631391), DOCK2 (rs77594147), SLC28A1 (rs28649017), ARHGAP5 (rs7151991), and CIITA (rs45601437) in the Asian comparison.
- Substituted cysteine accessibility method (SCAM) analysis of the transport domain of human concentrative nucleoside transporter 3 (hCNT3) and other family members reveals features of structural and functional importance.
Mulinta, The Journal of biological chemistry 2017 - GeneRIF: results that validate the newly developed structural homology model of CNT membrane architecture for human CNTs, revealed extended conformationally mobile regions within transport-domain TMs, identified pore-lining residues of functional importance, and provided evidence of an emerging novel elevator-type mechanism of transporter function.
- Defective hCNT1 transport contributes to gemcitabine chemoresistance in ovarian cancer subtypes: overcoming transport defects using a nanoparticle approach.
Hung, Cancer letters 2015 - GeneRIF: hCNT1 is a putative determinant for nucleoside analog chemoresistance in ovarian cancer.
- The oncogenic receptor ErbB2 modulates gemcitabine and irinotecan/SN-38 chemoresistance of human pancreatic cancer cells via hCNT1 transporter and multidrug-resistance associated protein MRP-2.
Skrypek, Oncotarget 2015 - GeneRIF: ErbB2 modulates gemcitabine and irinotecan/SN-38 chemoresistance of human pancreatic cancer cells via hCNT1 transporter and multidrug-resistance associated protein MRP-2.
- The MUC4 mucin mediates gemcitabine resistance of human pancreatic cancer cells via the Concentrative Nucleoside Transporter family.
Skrypek, Oncogene 2013 - GeneRIF: MUC4 and hCNT1 as potential targets to ameliorate the response of pancreatic tumors to gemcitabine treatment.
- Concentrative nucleoside transporter 1 (hCNT1) promotes phenotypic changes relevant to tumor biology in a translocation-independent manner.
Pérez-Torras, Cell death & disease 2013 - GeneRIF: Human CNT1 fits the profile of a transceptor in a substrate translocation-independent manner and is likely to be relevant to tumor biology.
- More
S28A1_HUMAN / O00337 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; hCNT1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Homo sapiens (Human) (see 9 papers)
TC 2.A.41.2.3 / O00337 Pyrimidine-preferring nucleoside:Na+ symporter, CNT1 (Na+/nucleoside = 2)(transports uridine, gemcitabine and 5'-deoxy-5'-fluorouridine) (Larráyoz et al., 2004), but in addition to pyrimidine nucelosides, it transports adenosine from Homo sapiens (Human) (see 5 papers)
NP_004204 sodium/nucleoside cotransporter 1 isoform 1 from Homo sapiens
35% identity, 63% coverage
- function: Sodium and pyrimidine nucleoside symporter of the plasma membrane that imports uridine, thymidine and cytidine into cells by coupling their transport to the transmembrane sodium electrochemical gradient. Also transports adenosine, an atypical substrate transported with high apparent affinity, but low maximum velocity. Therefore, exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (PubMed:10455109, PubMed:14701834, PubMed:15194733, PubMed:21795683, PubMed:21998139, PubMed:30658162, PubMed:32126230, PubMed:9124315). Involved in renal nucleoside (re)absorption (PubMed:30658162).
catalytic activity: uridine(out) + Na(+)(out) = uridine(in) + Na(+)(in) (RHEA:69887)
catalytic activity: thymidine(out) + Na(+)(out) = thymidine(in) + Na(+)(in) (RHEA:69891)
catalytic activity: cytidine(out) + Na(+)(out) = cytidine(in) + Na(+)(in) (RHEA:69895)
catalytic activity: adenosine(out) + Na(+)(out) = adenosine(in) + Na(+)(in) (RHEA:69927) - substrates: 5'-deoxy-5'-fluorouridine, Gemcitabine, Na+, Uridine
- Shotgun proteomics identification of proteins expressed in the Descemet's membrane of patients with Fuchs endothelial corneal dystrophy.
Nakagawa, Scientific reports 2023 - “...ENSG00000161904 Q8NC56 20.9 Angiopoietin-related protein 7 ANGPTL7 ENSG00000171819 O43827 18.8 Sodium/nucleoside cotransporter 1 SLC28A1 ENSG00000156222 O00337 15.7 EMILIN-2 EMILIN2 ENSG00000132205 Q9BXX0 15.2 Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial PDHA2 ENSG00000163114 P29803 15.0 Mucin-6 MUC6 ENSG00000184956 Q6W4X9 14.5 26S proteasome regulatory subunit 6A PSMC3...”
- Cryo-EM structure of the human concentrative nucleoside transporter CNT3.
Zhou, PLoS biology 2020 - “...(TIF) Click here for additional data file. S6 Fig Sequence alignment of hCNT1 (UniProt no. O00337), hCNT2 (UniProt no. O43868), hCNT3 (UniProt no. Q9HAS3), vcCNT (UniProt no. Q9KPL5), and CNTnw (UniProt no. G4CRQ5). Sequences were aligned with ClustalW. Invariant and highly conserved residues are shaded yellow...”
- Proteomic analysis to identify candidate biomarkers associated with type 1 diabetes
do, Diabetes, metabolic syndrome and obesity : targets and therapy 2018 - “...arylesterase 1 39,731 7/18 50.99 2,611.12 P04278 Sex hormone binding globulin 43,779 4/29 25.62 210.35 O00337 Sodium nucleoside cotransporter 71,584 3/35 3.39 102.95 Q13596 Sortingnexin1 5,907 3/40 11.88 178.19 O60235 Transmembrane protease serine 11D 46,264 2/20 11.48 122.25 P02766 Transthyretin 15,887 4/9 50.34 302.37 Q9NZQ9 Tropomodulin...”
- THE CONCISE GUIDE TO PHARMACOLOGY 2017/18: Transporters
Alexander, British journal of pharmacology 2017 - “...Solute carrier family 28 member 3 Systematic nomenclature SLC28A1 SLC28A2 SLC28A3 HGNC, UniProt SLC28A1 , O00337 SLC28A2 , O43868 SLC28A3 , Q9HAS3 Common abreviation CNT1 CNT2 CNT3 Substrates ribavirin [ 98 ], gemcitabine [ 97 ], zalcitabine , zidovudine cladribine [ 416 ], didanosine , vidarabine...”
- The Concise Guide to PHARMACOLOGY 2015/16: Transporters
Alexander, British journal of pharmacology 2015 - “...3 Systematic nomenclature SLC28A1 SLC28A2 SLC28A3 Common abreviation CNT1 CNT2 CNT3 HGNC, UniProt SLC28A1 , O00337 SLC28A2 , O43868 SLC28A3 , Q9HAS3 Substrates gemcitabine [ 90 ], zalcitabine , zidovudine cladribine [ 376 ], didanosine , vidarabine , fludarabine [ 298 ], formycin B [ 298...”
- The Concise Guide to PHARMACOLOGY 2013/14: transporters
Alexander, British journal of pharmacology 2013 - “...CNT2 CNT3 Systematic nomenclature SLC28A1 SLC28A2 SLC28A3 Common abbreviation CNT1 CNT2 CNT3 HGNC, UniProt SLC28A1, O00337 SLC28A2, O43868 SLC28A3, Q9HAS3 Endogenous substrates adenosine, uridine, thymidine, cytidine adenosine, inosine, guanosine, thymidine adenosine, inosine, uridine, guanosine, thymidine, cytidine Substrates gemcitabine, zidovudine, zalcitabine formycin B, cladribine, fludarabine, vidarabine, didanosine...”
- Comparative genomic and expression analysis of the adenosine signaling pathway members in Xenopus
Tocco, Purinergic signalling 2015 - “...NP_610447; SLC28A1-M.m: NP_001004184; SLC28A1-H.s: NP_004204; SLC28A2-G.g: XP_004943665 (isoform 6); SLC28A2-M.m: NP_766568; SLC28A2-H.s: NP_004203.2;...”
NP_001179096 solute carrier family 28 member 3 from Bos taurus
33% identity, 60% coverage
F1MGR1 Sodium/nucleoside cotransporter from Bos taurus
33% identity, 59% coverage
- Antiviral drug recognition and elevator-type transport motions of CNT3
Wright, Nature chemical biology 2024 - “...reconstitution of Bos taurus CNT3 Full length WT Bos taurus CNT3 sequence (bCNT3; uniprot ID: F1MGR1) was synthesized in the pFastBac vector containing a FLAG tag (DYKDDDDK) at the N-terminus of the protein. Baculovirus was generated in Spodoptera frugiperda sf9 insect cells using standard protocols and...”
- Differences in Milk Proteomic Profiles between Estrous and Non-Estrous Dairy Cows
Du, Animals : an open access journal from MDPI 2023 - “...SUMF2 1.39 U 0.03 Q2KJ93 Cell division control protein 42 homolog CDC42 1.32 U 0.03 F1MGR1 Sodium/nucleoside cotransporter SLC28A3 1.43 U 0.00 A0A3Q1M483 Acetyl-CoA carboxylase 1 ACACA 1.49 U 0.00 A0A3Q1M7Y8 Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 PLOD1 0.72 D 0.03 A0A3Q1NIJ7 Heparanase HPSE 0.64 D 0.00 A0A3Q1N0L4 Dynamin...”
8tz1A / F1MGR1 Cryo-em structure of bovine concentrative nucleoside transporter 3 in complex with ribavirin (see paper)
33% identity, 77% coverage
- Ligands: 1-(beta-d-ribofuranosyl)-1h-1,2,4-triazole-3-carboxamide; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (8tz1A)
S28A3_MOUSE / Q9ERH8 Solute carrier family 28 member 3; Concentrative Na(+)-nucleoside cotransporter 3; CNT 3; mCNT3 from Mus musculus (Mouse) (see paper)
TC 2.A.41.2.6 / Q9ERH8 Broad-specificity nucleoside:Na+, H+ and Li+ symporter, hCNT3 (Slc28a3) transports a broad range of both purine and pyrimidine nucleosides as well as anticancer and antiviral nucleoside drugs, but guanosine, 3'azido-3-deoxythymidine and 2',3'-dideoxycytidine, which are substrates with Na+, are not substrates with H+. Both of the two cation-binding sites can apparently bind Na+, but only one can bind H+, and the Na+ and H+ forms transport different ranges of substrates. (Note: Cnt1 and Cnt2 are Na+-specific.) from Homo sapiens (Human) (see 3 papers)
Slc28a3 / RF|NP_071712.3 solute carrier family 28 member 3 from Mus musculus (see paper)
32% identity, 59% coverage
- function: Sodium-dependent, pyrimidine- and purine-selective (PubMed:11032837). Involved in the homeostasis of endogenous nucleosides (PubMed:11032837). Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine) (PubMed:11032837). Employs a 2:1 sodium/nucleoside ratio (PubMed:11032837). Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (By similarity).
catalytic activity: thymidine(out) + 2 Na(+)(out) = thymidine(in) + 2 Na(+)(in) (RHEA:69899)
catalytic activity: cytidine(out) + 2 Na(+)(out) = cytidine(in) + 2 Na(+)(in) (RHEA:69903)
catalytic activity: uridine(out) + 2 Na(+)(out) = uridine(in) + 2 Na(+)(in) (RHEA:69907)
catalytic activity: adenosine(out) + 2 Na(+)(out) = adenosine(in) + 2 Na(+)(in) (RHEA:69911)
catalytic activity: guanosine(out) + 2 Na(+)(out) = guanosine(in) + 2 Na(+)(in) (RHEA:69915)
catalytic activity: inosine(out) + 2 Na(+)(out) = inosine(in) + 2 Na(+)(in) (RHEA:69919)
subunit: Homotrimer. - substrates: H+, Li+, Na+, Nucleosides
tcdb comment: (Na+/nucleoside = 2; Na+ + H+/nucleoside = 2; H+/nucleoside = 1). The matricellular protein, cysteine-rich angiogenic inducer 61 (CYR61), negatively regulates synthesis of the nucleoside transporters hENT1 and hCNT3, both of which transport the anti-cancer agent, gemcitabine (Hesler et al. 2016). Also probably transports gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine. Modeling revealed mobility of selected binding site and homotrimer interface residues (Latek 2017)
S28A3_RAT / Q8VIH3 Solute carrier family 28 member 3; Concentrative Na(+)-nucleoside cotransporter 3; CNT 3; rCNT3 from Rattus norvegicus (Rat) (see paper)
32% identity, 59% coverage
- function: Sodium-dependent, pyrimidine- and purine-selective. Involved in the homeostasis of endogenous nucleosides. Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine). Employs a 2:1 sodium/nucleoside ratio. Also able to transport gemcitabine, 3'- azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine.
catalytic activity: thymidine(out) + 2 Na(+)(out) = thymidine(in) + 2 Na(+)(in) (RHEA:69899)
catalytic activity: cytidine(out) + 2 Na(+)(out) = cytidine(in) + 2 Na(+)(in) (RHEA:69903)
catalytic activity: uridine(out) + 2 Na(+)(out) = uridine(in) + 2 Na(+)(in) (RHEA:69907)
catalytic activity: adenosine(out) + 2 Na(+)(out) = adenosine(in) + 2 Na(+)(in) (RHEA:69911)
catalytic activity: guanosine(out) + 2 Na(+)(out) = guanosine(in) + 2 Na(+)(in) (RHEA:69915)
catalytic activity: inosine(out) + 2 Na(+)(out) = inosine(in) + 2 Na(+)(in) (RHEA:69919)
subunit: Homotrimer.
XP_011517209 solute carrier family 28 member 3 isoform X2 from Homo sapiens
34% identity, 59% coverage
- The Role of RARG rs2229774, SLC28A3 rs7853758, and UGT1A6*4 rs17863783 Single-nucleotide Polymorphisms in the Doxorubicin-induced Cardiotoxicity in Solid Childhood Tumors.
Gündüz, Journal of pediatric hematology/oncology 2024 (PubMed)- GeneRIF: The Role of RARG rs2229774, SLC28A3 rs7853758, and UGT1A6*4 rs17863783 Single-nucleotide Polymorphisms in the Doxorubicin-induced Cardiotoxicity in Solid Childhood Tumors.
- Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters.
Yamamura, Biochemical and biophysical research communications 2021 (PubMed)- GeneRIF: Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters.
- Gene-Gene Interactions of Gemcitabine Metabolizing-Enzyme Genes hCNT3 and WEE1 for Preventing Severe Gemcitabine-Induced Hematological Toxicity.
Li, Journal of clinical pharmacology 2021 (PubMed)- GeneRIF: Gene-Gene Interactions of Gemcitabine Metabolizing-Enzyme Genes hCNT3 and WEE1 for Preventing Severe Gemcitabine-Induced Hematological Toxicity.
- Allosteric and transport modulation of human concentrative nucleoside transporter 3 at the atomic scale.
Duan, Physical chemistry chemical physics : PCCP 2021 (PubMed)- GeneRIF: Allosteric and transport modulation of human concentrative nucleoside transporter 3 at the atomic scale.
- Association of SLC28A3 Gene Expression and CYP2B6*6 Allele with the Response to Fludarabine Plus Cyclophosphamide in Chronic Lymphocytic Leukemia Patients.
Vukovic, Pathology oncology research : POR 2020 (PubMed)- GeneRIF: Association of SLC28A3 Gene Expression and CYP2B6*6 Allele with the Response to Fludarabine Plus Cyclophosphamide in Chronic Lymphocytic Leukemia Patients.
- Cryo-EM structure of the human concentrative nucleoside transporter CNT3.
Zhou, PLoS biology 2020 - GeneRIF: Cryo-EM structure of the human concentrative nucleoside transporter CNT3.
- Inward- and outward-facing homology modeling of human concentrative nucleoside transporter 3 (hCNT3) predicts an elevator-type transport mechanism.
Yao, Channels (Austin, Tex.) 2018 - GeneRIF: human CNT3 homology models generated validate previously published PCMBS SCAM data, and confirm an elevator-type mechanism of membrane transport
- Substituted cysteine accessibility method (SCAM) analysis of the transport domain of human concentrative nucleoside transporter 3 (hCNT3) and other family members reveals features of structural and functional importance.
Mulinta, The Journal of biological chemistry 2017 - GeneRIF: results that validate the newly developed structural homology model of CNT membrane architecture for human CNTs, revealed extended conformationally mobile regions within transport-domain TMs, identified pore-lining residues of functional importance, and provided evidence of an emerging novel elevator-type mechanism of transporter function.
- More
S28A3_HUMAN / Q9HAS3 Solute carrier family 28 member 3; Concentrative Na(+)-nucleoside cotransporter 3; CNT 3; hCNT3 from Homo sapiens (Human) (see 8 papers)
TC 2.A.41.2.8 / Q9HAS3 Solute carrier family 28 member 3 (Concentrative Na+-nucleoside cotransporter 3) (CNT 3) (hCNT3) from Homo sapiens (see 9 papers)
34% identity, 52% coverage
- function: Sodium-dependent, pyrimidine- and purine-selective (PubMed:11032837, PubMed:15861042, PubMed:16446384, PubMed:17140564, PubMed:21998139). Involved in the homeostasis of endogenous nucleosides (PubMed:11032837, PubMed:15861042). Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine) (PubMed:11032837). Employs a 2:1 sodium/nucleoside ratio (PubMed:11032837). Transports uridine (PubMed:21795683). Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (PubMed:11032837, PubMed:17140564).
catalytic activity: thymidine(out) + 2 Na(+)(out) = thymidine(in) + 2 Na(+)(in) (RHEA:69899)
catalytic activity: cytidine(out) + 2 Na(+)(out) = cytidine(in) + 2 Na(+)(in) (RHEA:69903)
catalytic activity: uridine(out) + 2 Na(+)(out) = uridine(in) + 2 Na(+)(in) (RHEA:69907)
catalytic activity: adenosine(out) + 2 Na(+)(out) = adenosine(in) + 2 Na(+)(in) (RHEA:69911)
catalytic activity: guanosine(out) + 2 Na(+)(out) = guanosine(in) + 2 Na(+)(in) (RHEA:69915)
catalytic activity: inosine(out) + 2 Na(+)(out) = inosine(in) + 2 Na(+)(in) (RHEA:69919)
catalytic activity: [Isoform 1]: uridine(out) + 2 Na(+)(out) = uridine(in) + 2 Na(+)(in) (RHEA:69907)
catalytic activity: [Isoform 2]: uridine(out) + 2 Na(+)(out) = uridine(in) + 2 Na(+)(in) (RHEA:69907)
subunit: Homotrimer. - substrates: Na+, Nucleosides
tcdb comment: This protein is distinct from TC# 2.A.41.2.6 (78% identity) although these two proteins are called Slc28a3 and CNT3 and have the same description in UniProt (see 2.A.41.2.6 for a more complete description) - Network pharmacology combined with GEO database identifying the mechanisms and molecular targets of Polygoni Cuspidati Rhizoma on Peri-implants
Shan, Scientific reports 2022 - “...cGMP 3',5'-cyclic phosphodiesterase 10A CA14 Q9ULX7 Carbonic anhydrase 14 PIM2 Q9P1W9 Serine/threonine-protein kinase pim-2 SLC28A3 Q9HAS3 Solute carrier family 28 member 3 SLC22A12 Q96S37 Solute carrier family 22 member 12 ESR2 Q92731 Estrogen receptor beta NEU4 Q8WWR8 Sialidase-4 ST3GAL2 Q16842 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2 EPHA7 Q15375 Ephrin type-A...”
- Systems pharmacology-based drug discovery and active mechanism of natural products for coronavirus pneumonia (COVID-19): An example using flavonoids
Wang, Computers in biology and medicine 2022 - “...P21964 83 IL1A P01583 134 SLC6A4 P31645 33 COQ8B Q96D53 84 IL1 P01584 135 SLC28A3 Q9HAS3 34 CREB1 P16220 85 IL2 P60568 136 SLC29A1 Q99808 35 CRP P02741 86 IL4 P05112 137 SOD1 P00441 36 CTSB P07858 87 IL6 P05231 138 STAT1 P42224 37 CTSL P07711...”
- Cryo-EM structure of the human concentrative nucleoside transporter CNT3.
Zhou, PLoS biology 2020 - “...Fig Sequence alignment of hCNT1 (UniProt no. O00337), hCNT2 (UniProt no. O43868), hCNT3 (UniProt no. Q9HAS3), vcCNT (UniProt no. Q9KPL5), and CNTnw (UniProt no. G4CRQ5). Sequences were aligned with ClustalW. Invariant and highly conserved residues are shaded yellow and gray, respectively. Residues responsible for substrate binding...”
- Human Concentrative Nucleoside Transporter 3 (hCNT3, SLC28A3) Forms a Cyclic Homotrimer
Stecula, Biochemistry 2017 - “...CNT3 cDNA was cloned into the pcDNA5/FRT mammalian expression vector (Invitrogen V601020). SLC28A3 (UniProt ID Q9HAS3) cDNA were obtained from GE Dharmacon MGC cDNAs collection (MHS6278202857241). Locations of cysteine mutants were selected based on the comparative structure models. Standard protocols for QuikChange II site-directed mutagenesis were...”
- THE CONCISE GUIDE TO PHARMACOLOGY 2017/18: Transporters
Alexander, British journal of pharmacology 2017 - “...Systematic nomenclature SLC28A1 SLC28A2 SLC28A3 HGNC, UniProt SLC28A1 , O00337 SLC28A2 , O43868 SLC28A3 , Q9HAS3 Common abreviation CNT1 CNT2 CNT3 Substrates ribavirin [ 98 ], gemcitabine [ 97 ], zalcitabine , zidovudine cladribine [ 416 ], didanosine , vidarabine , fludarabine [ 329 ], formycin...”
- Systems Pharmacological Approach of Pulsatillae Radix on Treating Crohn's Disease.
Suh, Evidence-based complementary and alternative medicine : eCAM 2017 - “...receptor RXR-alpha RXRA Q14524 Sodium channel protein type 5 subunit alpha SCN5A P16581 E-selectin SELE Q9HAS3 Solute carrier family 28 member 3 SLC28A3 O43826 Glucose-6-phosphate translocase SLC37A4 P13866 Sodium/glucose cotransporter 1 SLC5A1 P31639 Sodium/glucose cotransporter 2 SLC5A2 Q9NY91 Low affinity sodium-glucose cotransporter SLC5A4 P23975 Sodium-dependent noradrenaline...”
- Rosetta Broker for membrane protein structure prediction: concentrative nucleoside transporter 3 and corticotropin-releasing factor receptor 1 test cases.
Latek, BMC structural biology 2017 - “...hCNT3 monomer only the fragment of the full 691-residue long sequence of hCNT3 (Uniprot id: Q9HAS3) was used. Namely, the N and C-terminus which were predicted to be outside the membrane (see Uniprot) were cut out leaving the 522-residue hCNT3 sequence (see Fig. 2e ) corresponding...”
- “...The consensus membrane topology predictor TOPCONS [ 31 ] and the hCNT3 Uniprot entry (id: Q9HAS3) were used to detect positions of three N-terminal transmembrane helices (TMHs) (see Fig. 2e ). Additionally, the sequence profile-based lipophilicity prediction was performed and used in the Broker simulation. During...”
- The Concise Guide to PHARMACOLOGY 2015/16: Transporters
Alexander, British journal of pharmacology 2015 - “...Common abreviation CNT1 CNT2 CNT3 HGNC, UniProt SLC28A1 , O00337 SLC28A2 , O43868 SLC28A3 , Q9HAS3 Substrates gemcitabine [ 90 ], zalcitabine , zidovudine cladribine [ 376 ], didanosine , vidarabine , fludarabine [ 298 ], formycin B [ 298 ] zalcitabine , formycin B ,...”
- More
cntA nucleoside transporter (Eurofung) from Emericella nidulans (see paper)
XP_663097 protein cntA from Aspergillus nidulans FGSC A4
33% identity, 66% coverage
AFUA_6G13190 H+/nucleoside cotransporter from Aspergillus fumigatus Af293
34% identity, 65% coverage
- Characterization of gprK Encoding a Putative Hybrid G-Protein-Coupled Receptor in Aspergillus fumigatus
Jung, PloS one 2016 - “...5742705 AFUA_4G14230 MFS transporter, putative -2.932 0.0228129 5738847 AFUA_3G09390 AMMECR1 family protein -2.899 0.0389796 5748265 AFUA_6G13190 H+/nucleoside cotransporter -2.878 0.0455004 5741172 AFUA_4G06620 Glu/Leu/Phe/Val dehydrogenase, putative -2.634 0.0089898 5745210 AFUA_5G12035 conserved hypothetical protein -2.617 0.0374917 5743818 AFUA_5G05610 cell cycle control protein Cwf14/Bud31 -2.599 0.0147689 5735341 AFUA_2G09860 purine-cytosine...”
NCU08148 H+/nucleoside cotransporter from Neurospora crassa OR74A
33% identity, 65% coverage
- DNA affinity purification sequencing and transcriptional profiling reveal new aspects of nitrogen regulation in a filamentous fungus
Huberman, Proceedings of the National Academy of Sciences of the United States of America 2021 (secret) - Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa
Watters, G3 (Bethesda, Md.) 2018 - “...NCU08147 11256 Na or K P-type ATPase ph7 Morph/Hyph Transporter Activity/Catalytic Activity Cellular Process/Metabolic Process NCU08148 22001 H+/nucleoside cotransporter E. coli CS Orth Transporter Activity Localization Process/Cellular Process NCU08225 11303 high affinity nickel transporter nic1 trm-34 Morph/Hyph NCU08289 11100 DNA methylation modulator-2 dmm-2 Transc Factors Binding...”
- Identification of the CRE-1 cellulolytic regulon in Neurospora crassa
Sun, PloS one 2011 - “...encoded predicted sugar transporters, (NCU01633, NCU04537, NCU10021, NCU00821, and NCU05627), one encoded a nucleoside transporter (NCU08148), several genes had annotation suggesting a role in alternative carbon source utilization (e.g. pyruvic acid), and 26 genes encoded putative or hypothetical proteins. No cellulolytic genes were induced in the...”
AO090003000443 No description from Aspergillus oryzae RIB40
31% identity, 67% coverage
BTF1_06840 NupC/NupG family nucleoside CNT transporter from Bacillus thuringiensis HD-789
31% identity, 97% coverage
- NagRBt Is a Pleiotropic and Dual Transcriptional Regulator in Bacillus thuringiensis
Cao, Frontiers in microbiology 2018 - “...(DEGs) in Bti75- nagR . Cytidine deaminase (BTF1_06850), pyrimidine-nucleoside phosphorylase (BTF1_06845), and pyrimidine nucleoside transporter (BTF1_06840) involved in nucleotide metabolism might be on the same operon. The expression of genes, lipoprotein (BTF1_26870), acyl-dehydrogenase (BTF1_25115 and BTF1_08960), and acyl carrier protein (BTF1_17540) involved in fatty acid metabolism...”
- “...9.8 20.046 6.046 0.280 BTF1_01615 licR BigG family transcription antiterminator / / / 7.751 NT BTF1_06840 nupC nucleoside transporter NupC / / / 4.738 NT BTF1_06845 pdp Pyrimidine-nucleoside phosphorylase / / / 14.614 NT BTF1_06850 cdd Cytidine deaminase / / / 19.753 45.010 2.977 BTF1_07625 Hypothetical...”
VDAG_10194 sodium/nucleoside cotransporter from Verticillium dahliae VdLs.17
31% identity, 62% coverage
- Hypothetical Protein VDAG_07742 Is Required for Verticillium dahliae Pathogenicity in Potato
Wang, International journal of molecular sciences 2023 - “...conidia was performed by qRT-PCR, for which, the following 10 upregulated proteins were randomly selected: VDAG_10194, VDAG_02304, VDAG_10208, VDAG_07442, VDAG_09254, VDAG_08248, VDAG_07373, VDAG_03665, VDAG_01700, and VDAG_09741. Basic information describing these proteins and primers used for qRT-PCR verification are listed in Tables S1 and S6 , respectively....”
- “...lyase B 6.385 0.02084166 VDAG_02304 Aminopeptidase Y 5.838 0.00167567 VDAG_01113 EFG_II domain-containing protein(predicted) 5.636 0.00489088 VDAG_10194 Sodium/nucleoside cotransporter 5.626 0.00236321 VDAG_10460 Amino-acid permease inda1 5.549 0.00079813 VDAG_05115 Zinc carboxypeptidase A 5.42 0.00334736 VDAG_07742 Podospora anserina S mat+ genomic DNA chromosome 6, supercontig 4 (predicted) 5.4 0.00634638...”
NUPC_BACSU / P39141 Nucleoside permease NupC from Bacillus subtilis (strain 168) (see paper)
TC 2.A.41.1.2 / P39141 Pyrimidine-specific nucleoside:H+ symporter, NupC from Bacillus subtilis (see 4 papers)
32% identity, 97% coverage
- function: Transport of the pyrimidine nucleoside uridine.
- substrates: H+, Pyrimidine
BA0332 nucleoside transporter, NupC family from Bacillus anthracis str. Ames
31% identity, 97% coverage
BC0363 Nucleoside permease nupC from Bacillus cereus ATCC 14579
BMB171_RS01930 NupC/NupG family nucleoside CNT transporter from Bacillus thuringiensis BMB171
31% identity, 97% coverage
- Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus
Caro-Astorga, NPJ biofilms and microbiomes 2020 - “...required for nucleoside synthesis (BC0403*, BC0639, BC0640*, BC2383 , BC3939*, BC2790), nucleoside transporters ( BC2973, BC0363 ) and specific phosphate transporters ( BC4265-68 and BC0711 ), all molecules required for DNA synthesis. These results suggest that these metabolic changes might be, among others, oriented to the...”
- Two Putative Polysaccharide Deacetylases Are Required for Osmotic Stability and Cell Shape Maintenance in Bacillus anthracis
Arnaouteli, The Journal of biological chemistry 2015 - “...flanked by the putative aminopeptidase genes ba0329 and ba0360 and NupC-like nucleoside transporters ba0332 and bc0363. Arrows indicate open reading frames. Homologous genes are indicated with the same shading. C, SDS-PAGE of the purified putative PDAs BA0330 and BA0331. For BA0330: lane 1 , molecular weight...”
- NupR Responding to Multiple Signals Is a Nucleoside Permease Regulator in Bacillus thuringiensis BMB171
Qin, Microbiology spectrum 2022 - “...TABLE1 Genes directly regulated by NagR2 Genetic locus tag Description Fold change Nup sequence Position BMB171_RS01930 Nucleoside permease 5.027 AAAGGTCAGACATCTTACGT Pro a BMB171_RS09485 L-Lactate dehydrogenase 2.026 AGAAGTAAGACGTATTATCT Pro BMB171_RS25245 Nucleoside transporter NupC family 5.358 AATGGTCAGACGTCATTCGT Pro BMB171_RS25250 Nucleoside transporter NupC family 5.034 AATGGTCAGACGTTCAACGT Pro BMB171_RS26110 Nucleoside...”
SACOL0310 nucleoside permease NupC, putative from Staphylococcus aureus subsp. aureus COL
SAOUHSC_00293 hypothetical protein from Staphylococcus aureus subsp. aureus NCTC 8325
SAUSA300_0313 putative nucleoside permease NupC from Staphylococcus aureus subsp. aureus USA300_FPR3757
USA300HOU_RS01665 NupC/NupG family nucleoside CNT transporter from Staphylococcus aureus subsp. aureus USA300_TCH1516
28% identity, 97% coverage
- Transcriptomic and Metabolomic Analysis of a Fusidic Acid-Selected fusA Mutant of Staphylococcus aureus
Gupta, Antibiotics (Basel, Switzerland) 2022 - “...Transport and binding proteins SACOL1144 smpB probable transmembrane protein SmpB iron compound ABC transporter 23.21 SACOL0310 NA nucleoside permease NupC, putative 11.35 SACOL1476 NA basic amino acid/polyamine antiporter, APA family 10.63 SACOL2525 NA lantibiotic ABC transporter ATP-binding protein 12.61 SACOL2386 narK nitrite extrusion protein 14.55 antibiotics-11-01051-t006_Table...”
- In vitro and in vivo models of Staphylococcus aureus endophthalmitis implicate specific nutrients in ocular infection
Sadaka, PloS one 2014 - “...54.8 (1.5) 40.1 (1.8) SACOL0309 ( yeiN ) Conserved hypothetical protein 45.3 (1.6) 38.7 (1.7) SACOL0310 ( yeiM ) Nucleoside permease NupC, putative 28.3 (2.1) 32.0 (1.7) SACOL0311 nanT Sodium:solute symporter family protein 38.1 (1.7) 13.2 (1.7) SACOL0312 nanA N-acetylneuraminate lyase 54.8 (1.7) 3.5 (1.3) 14.4...”
- “...5.0 (3.2) SACOL0308 c Carbohydrate kinase (3 region) 7.2 (1.7) SACOL0309 Hypothetical protein 6.8 (1.7) SACOL0310 Nucleoside permease NupC 7.2 (2.0) SACOL0427 Hypothetical protein 7.5 (1.1) SACOL0428 metE 5-methyltetrahydropteroyltriglutamate/homocysteineS-methyltransferase 19.7 (1.2) 9.2 (1.6) SACOL0429 Bifunctional homocysteineS-methyltransferase/5,10-methylenetetrahydrofolate reductase 28.3 (1.2) 11.5 (1.4) SACOL0430 Trans-sulfuration enzyme family protein...”
- Inactivation of thyA in Staphylococcus aureus attenuates virulence and has a strong impact on metabolism and virulence gene expression
Kriegeskorte, mBio 2014 - “...the microarray analysis, there was a strong upregulation of SACOL0566 ( nupC ), while the SACOL0310 and SACOL0701 genes were unaffected. To confirm the biological function of nupC as a pyrimidine transporter, which is responsible for the uptake of extracellular thymidine, we tested a nupC mutant...”
- Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure
Yang, Frontiers in microbiology 2021 - “.../ / Non-coding region 0 and 6 g/mL 306,103 44.68014295 I < - > I SAOUHSC_00293 Hypothetical protein 0 and 6 g/mL 454,194 47.07477546 / / Non-coding region 0 and 6 g/mL 768,340 46.38076319 T < - > A SAOUHSC_00786 Hypothetical protein 0 and 6 g/mL...”
- Role of the accessory gene regulator agr in community-associated methicillin-resistant Staphylococcus aureus pathogenesis
Cheung, Infection and immunity 2011 - “...SAUSA300_0220 SAUSA300_0221 SAUSA300_0311 SAUSA300_0312 SAUSA300_0313 SAUSA300_0863 SAUSA300_0864 SAUSA300_1062 SAUSA300_1063 SAUSA300_1712 SAUSA300_1713...”
- Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA
Xu, Frontiers in microbiology 2016 - “...4.71E-03 Phosphate ABC superfamily ATP binding cassette transporter, membrane protein USA300HOU_RS01225 2.26 9.38E-03 Hypothetical protein USA300HOU_RS01665 2.29 2.74E-03 Possible CNT family concentrative nucleoside transporter USA300HOU_RS13665 2.29 3.12E-03 MarR family transcriptional regulator USA300HOU_RS13095 hlgA 2.30 8.97E-04 Gamma-hemolysin component A USA300HOU_RS01655 2.35 2.51E-03 PfkB family carbohydrate kinase USA300HOU_RS03725...”
SAFDA_0271 NupC/NupG family nucleoside CNT transporter from Staphylococcus aureus
28% identity, 97% coverage
RSAU_000259 NupC/NupG family nucleoside CNT transporter from Staphylococcus aureus subsp. aureus 6850
28% identity, 97% coverage
SA0302 probable pyrimidine nucleoside transport protein from Staphylococcus aureus subsp. aureus N315
28% identity, 97% coverage
- Crystal structure of the CN-hydrolase SA0302 from the pathogenic bacterium Staphylococcus aureus belonging to the Nit and NitFhit Branch of the nitrilase superfamily
Gordon, Journal of biomolecular structure & dynamics 2013 (PubMed)- “...http://www.tandfonline.com/loi/tbsd20 Crystal structure of the CN-hydrolase SA0302 from the pathogenic bacterium Staphylococcus aureus belonging to the Nit and...”
- “...Y. Chirgadze (2013) Crystal structure of the CN-hydrolase SA0302 from the pathogenic bacterium Staphylococcus aureus belonging to the Nit and NitFhit Branch of...”
- Direct targets of CodY in Staphylococcus aureus
Majerczyk, Journal of bacteriology 2010 - “...oppB oppC oppD oppBCDF, SA0995 oppC SA0995 SA2476 SA0302 SA0171 SA2619 SA1963 SA0010 SA0011 SA2476-SA2471 brnQ2 brnQ1 PTS SA0228 SA0230 Other transporters...”
- Genome-wide transcriptional profiling of the response of Staphylococcus aureus to cryptotanshinone
Feng, Journal of biomedicine & biotechnology 2009 - “...SA1831, srtB , glpT , oppF , SA2135, msmX , opp-1F , dps , and SA0302). In general, there were positive correlations between microarray data and real-time RT-PCR data for all 13 genes ( Table 3 ). However, expression of the genes SA1831 and SA0302 was...”
- “...SA0206 GACGCTGTCCACCAGATAA opp-1Ffor SA2251 TCATCATTACACCCATTTC opp-1Frev SA2251 GCCTTAGATAGACCGACTT dpsfor SA1941 TTAGCGGTAGGAGGAAAC dpsrev SA1941 ATCATCGCCAGCATTACC SA0302for SA0302 GAATGGAAAAACAGGAAAAC SA0302rev SA0302 GCAAACACATAGCCAATAAG (a) ORF, open reading frame. (b) refer to references [ 14 ]. Table 3 Real-time RT-PCR analysis of gene expression. N315 ORF N315 gene N315 description...”
- Effect of a glucose impulse on the CcpA regulon in Staphylococcus aureus
Seidl, BMC microbiology 2009 - “...contained putative cre -sites in their promoter regions, or as in the case of SA0186, SA0302, and gntP , belonged to an operon which contained a putative cre -site and were probably under the direct control of CcpA. The up-regulation of the glucose uptake protein homologue...”
SACOL0566 nucleoside permease NupC from Staphylococcus aureus subsp. aureus COL
SA0479 pyrimidine nucleoside transport protein from Staphylococcus aureus subsp. aureus N315
SAV0521 pyrimidine nucleoside transport protein from Staphylococcus aureus subsp. aureus Mu50
MW0476 pyrimidine nucleoside transport protein from Staphylococcus aureus subsp. aureus MW2
SAR0524 nucleoside permease from Staphylococcus aureus subsp. aureus MRSA252
30% identity, 95% coverage
- Binding of Cyclic Di-AMP to the Staphylococcus aureus Sensor Kinase KdpD Occurs via the Universal Stress Protein Domain and Downregulates the Expression of the Kdp Potassium Transporter
Moscoso, Journal of bacteriology 2016 - “..., covering 35% of its sequence. Despite the lower levels of similarity of SACOL0066 and SACOL0566 to USPs, they were also included in the analysis. To investigate if SACOL0066, SACOL0566, Usp1 (SACOL1753), or Usp2 (SACOL1759) was able to bind c-di-AMP, we used an S. aureus ORFeome...”
- Inactivation of thyA in Staphylococcus aureus attenuates virulence and has a strong impact on metabolism and virulence gene expression
Kriegeskorte, mBio 2014 - “...diphosphate kinase 3.77 SACOL1518 cmk Cytidylate kinase 2.96 2.80 SACOL2111 tdk Thymidine kinase 4.75 3.29 SACOL0566 nupC Nucleoside permease NupC 6.88 5.14 Nucleosides, purines, and pyrimidines SACOL1211 uraA Uracil permease 16.43 17.81 a All values shown were filtered to be statistically significant with P values of...”
- “...function as nucleoside transporters has not been proven experimentally for S.aureus , we assumed that SACOL0566 functions as the primary pyrimidine transporter in S.aureus due to its high homology to nupC from Bacillus subtilis ( 55 ). In the microarray analysis, there was a strong upregulation...”
- Investigating the genetic regulation of the ECF sigma factor σS in Staphylococcus aureus
Burda, BMC microbiology 2014 - “...551 insertion site Gene Hits b Unique c DNA metabolism: DNA replication, recombination and repair SACOL0566 NE544 Nucleoside permease nupC 1 1 Regulators SACOL0513 NE1883 Transcriptional regulatory protein glcT 1 1 SACOL1451 NE1684 Response regulator arlR 1 1 SACOL1436 NE9 Modulator of SarA msa 1 1...”
- Prevalence and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Pigs in Japan
Kawanishi, Antibiotics (Basel, Switzerland) 2024 - “...chromosomes of the methicillin-susceptible S. aureus strain (ST398) isolated from human patients in Japan (SA0052, SA0479, and SA2854) and the MRSA strain isolated from pigs in Europe (55-100-120 and 55-103-051) and USA (ISU933 and P23-03_SW181_1) were used ( Supplementary Table S1 ). Gubbins (version 2.3.4) (...”
- Transcriptional profiling analysis of the global regulator NorG, a GntR-like protein of Staphylococcus aureus
Truong-Bolduc, Journal of bacteriology 2011 - “...and membrane biogenesis SA0238 SA1164 SA1168 SA0479 SA1932 Teichoic acid biosynthesis protein, putative Fibrinogen binding-related protein Fibrinogen-binding...”
- Overexpression of genes of the cell wall stimulon in clinical isolates of Staphylococcus aureus exhibiting vancomycin-intermediate- S. aureus-type resistance to vancomycin
McAleese, Journal of bacteriology 2006 - “...SA2252 SA2253 SA2254 SA0242 SA1718 SA1970 SA0022 SA0131 SA0479 SA0600 SA1041 SA1042 SA0298 SA0325 SA1992 SA1993 SA1994 SA1995 SA1996 SA1997 SA2241 SA2294 SA0204...”
- Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures
Even, Applied and environmental microbiology 2009 - “...Nucleotide and nucleic acid metabolism genes SAV1065 SAV0521 DNA replication, recombination, and repair genes SAV1146 SAV1252 SAV0432 SAV2550 MW2471 feoB...”
- Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures
Even, Applied and environmental microbiology 2009 - “...MW0010 MW2304 gltT SAV1255 MW1138 codY MW0948 purK MW0476 nupC MW1029 MW1135 uvrC xerC MW0393 hsdS Secretion genes SAV0964 SAV2230 SAV0778 MW0846 MW2149 MW0740...”
- The Staphylococcus aureus response to unsaturated long chain free fatty acids: survival mechanisms and virulence implications
Kenny, PloS one 2009 - “...2.00 2.27E-03 SAR0261 putative nitric oxide reductase 2.22 6.16E-04 SAR0302 putative formate/nitrite transporter 2.38 8.03E-03 SAR0524 nupC nucleoside permease 2.94 3.96E-03 SAR0562 putative deoxyadenosine kinase protein 2.17 2.64E-02 SAR0563 putative deaminase 2.50 3.75E-03 SAR0569 putative glycosyl transferase 2.13 4.40E-03 SAR0642 ABC transporter permease protein 2.56 9.65E-03...”
CPE1284 pyrimidine nucleoside transporter from Clostridium perfringens str. 13
31% identity, 97% coverage
EQ812_06755 NupC/NupG family nucleoside CNT transporter from Staphylococcus lugdunensis
28% identity, 98% coverage
- Comparative proteomics analysis of biofilms and planktonic cells of Enterococcus faecalis and Staphylococcus lugdunensis with contrasting biofilm-forming ability
Cho, PloS one 2024 - “...EQ_ EQ812_05590 ), APC family permease ( EQ812_06165 ), NupC/NupG family nucleoside CNT transporter ( EQ812_06755 ), etc., to membrane, transmembrane or transmembrane helix. The resulting pathway networks were analyzed as depicted in Fig 3a and 3b . For both microorganisms, most annotated proteins were associated...”
- “...3 61.72839506 Serine protease EF_1817 S . lugdunensis 9 495 5.347593583 Transmembrane helix EQ812_05590 EQ812_06165 EQ812_06755 EQ812_02465 EQ812_07275 EQ812_03435 EQ812_04050 EQ812_09000 EQ812_08585 9 498 5.315379164 Transmembrane EQ812_05590 EQ812_06165 EQ812_06755 EQ812_02465 EQ812_07275 EQ812_03435 EQ812_04050 EQ812_09000 EQ812_08585 9 525 5.042016807 Membrane EQ812_05590 EQ812_06165 EQ812_06755 EQ812_02465 EQ812_07275 EQ812_03435 EQ812_04050...”
CLAU_3830 NupC/NupG family nucleoside CNT transporter from Clostridium autoethanogenum DSM 10061
30% identity, 97% coverage
BAS4922 nucleoside transporter, NupC family from Bacillus anthracis str. Sterne
AW20_3236 NupC/NupG family nucleoside CNT transporter from Bacillus anthracis str. Sterne
30% identity, 93% coverage
- Beyond the spore, the exosporium sugar anthrose impacts vegetative Bacillus anthracis gene regulation in cis and trans
Norris, Scientific reports 2023 - “...system, trehalose-specific IIBC component 1.26 AW20_5282 BAS2949 Magnesium and cobalt transport protein CorA 1.33 AW20_3236 BAS4922 Nucleoside transporter, NupC family 1.45 AW20_2625 BAS0165 GntP; Gluconate permease, Bsu4004 homolog 1.45 AW20_2115 BAS0599 TreC; Trehalose-6-phosphate hydrolase 1.46 AW20_453 BAS2203 Oxalate/formate antiporter 1.57 AW20_2626 BAS0164 FGGY family of carbohydrate...”
- Beyond the spore, the exosporium sugar anthrose impacts vegetative Bacillus anthracis gene regulation in cis and trans
Norris, Scientific reports 2023 - “...PTS system, trehalose-specific IIBC component 1.26 AW20_5282 BAS2949 Magnesium and cobalt transport protein CorA 1.33 AW20_3236 BAS4922 Nucleoside transporter, NupC family 1.45 AW20_2625 BAS0165 GntP; Gluconate permease, Bsu4004 homolog 1.45 AW20_2115 BAS0599 TreC; Trehalose-6-phosphate hydrolase 1.46 AW20_453 BAS2203 Oxalate/formate antiporter 1.57 AW20_2626 BAS0164 FGGY family of...”
BMB171_RS25245 NupC/NupG family nucleoside CNT transporter from Bacillus thuringiensis BMB171
30% identity, 93% coverage
- NupR Responding to Multiple Signals Is a Nucleoside Permease Regulator in Bacillus thuringiensis BMB171
Qin, Microbiology spectrum 2022 - “...sequence Position BMB171_RS01930 Nucleoside permease 5.027 AAAGGTCAGACATCTTACGT Pro a BMB171_RS09485 L-Lactate dehydrogenase 2.026 AGAAGTAAGACGTATTATCT Pro BMB171_RS25245 Nucleoside transporter NupC family 5.358 AATGGTCAGACGTCATTCGT Pro BMB171_RS25250 Nucleoside transporter NupC family 5.034 AATGGTCAGACGTTCAACGT Pro BMB171_RS26110 Nucleoside transporter NupC family 5.397 GGAGGTCAAACGTCTAATGA Pro BMB171_RS26735 Bacillolysin 1.452 AGTGGTATGACAACTCAAAA Pro BMB171_RS26950 HAD...”
EW136_00935 NupC/NupG family nucleoside CNT transporter from Staphylococcus pseudintermedius
28% identity, 98% coverage
- Staphylococcal Protein A (spa) Locus Is a Hot Spot for Recombination and Horizontal Gene Transfer in Staphylococcus pseudintermedius
Zukancic, mSphere 2020 - “...CP035741 type) contains three putative genes annotated as encoding NupC/NupG family nucleoside CNT transporter (locus_tag EW136_00935 ), a pseudouridine-5-phosphate glycosidase (locus_tag EW136_00940 ), and a winged helix-turn-helix transcriptional regulator (locus_tag EW136_00945 ). The spa -positive (ST45_1) and spa -negative (ST45_2) sublineages of ST45 are also indicated....”
- “...steps ( 43 , 44 ). In locus type VIII, a set of three genes, EW136_00935 (nucleoside transporter protein, COG1972, functional category F), EW136_00940 (pseudouridine-5-phosphate glycosidase, COG2313, functional category F), and EW136_00945 (winged helix-turn-helix transcriptional regulator), were integrated. Overall, the spa -negative strains have lineage-specific genes...”
CNT cation-coupled nucleoside transporter (H(+)/nucleoside symporter) from Candida albicans (see 5 papers)
27% identity, 66% coverage
- CharProtDB CGD description: Cation-coupled nucleoside transporter (H(+)/nucleoside symporter); CNT family; transports adenosine, uridine, inosine, guanosine, tubercidin; variant alleles encode high- and low-affinity isoforms; S or G at residue 328 affects specificity
BMB171_RS25250 NupC/NupG family nucleoside CNT transporter from Bacillus thuringiensis BMB171
29% identity, 93% coverage
- NupR Responding to Multiple Signals Is a Nucleoside Permease Regulator in Bacillus thuringiensis BMB171
Qin, Microbiology spectrum 2022 - “...a BMB171_RS09485 L-Lactate dehydrogenase 2.026 AGAAGTAAGACGTATTATCT Pro BMB171_RS25245 Nucleoside transporter NupC family 5.358 AATGGTCAGACGTCATTCGT Pro BMB171_RS25250 Nucleoside transporter NupC family 5.034 AATGGTCAGACGTTCAACGT Pro BMB171_RS26110 Nucleoside transporter NupC family 5.397 GGAGGTCAAACGTCTAATGA Pro BMB171_RS26735 Bacillolysin 1.452 AGTGGTATGACAACTCAAAA Pro BMB171_RS26950 HAD superfamily hydrolase 1.707 AATAGTAAGACATCAAACCT Pro BMB171_RS18795 GntR family...”
nupC / CAA52821.1 NupC from Escherichia coli (see paper)
28% identity, 96% coverage
ECs3272 permease of transport system for 3 nucleosides from Escherichia coli O157:H7 str. Sakai
28% identity, 96% coverage
Cru / b2393 nucleoside:H+ symporter NupC from Escherichia coli K-12 substr. MG1655 (see 6 papers)
nupC / P0AFF2 nucleoside:H+ symporter NupC from Escherichia coli (strain K12) (see 13 papers)
NUPC_ECOLI / P0AFF2 Nucleoside permease NupC; Nucleoside-transport system protein NupC from Escherichia coli (strain K12) (see 6 papers)
TC 2.A.41.1.1 / P0AFF2 Pyrimidine nucleoside:H+ symporter, NupC (Craig et al. 1994; Patching et al. 2005). Wild-type NupC had an apparent affinity for uridine of 22.2 +/- 3.7 muM and an apparent binding affinity of 1.8-2.6 mM, and various mutants with alterred properties were isolated and characterized (Sun et al. 2017). ADP-glucose is also a substrate of this system from Escherichia coli (see 5 papers)
b2393 nucleoside (except guanosine) transporter from Escherichia coli str. K-12 substr. MG1655
EC958_5013, ETEC_2511, SF5M90T_2387 nucleoside permease NupC from Escherichia coli O25b:H4-ST131
28% identity, 96% coverage
- function: Nucleoside transport protein that can transport adenosine, uridine, thymidine, cytidine and deoxycytidine (PubMed:11466294, PubMed:14668133, PubMed:15678184, PubMed:374403). Shows weak activity with inosine and xanthosine (PubMed:11466294, PubMed:14668133). Transport is driven by a proton motive force (PubMed:14668133, PubMed:374403). Does not transport guanosine, deoxyguanosine, hypoxanthine or uracil (PubMed:14668133, PubMed:374403). Also shows activity with the chemotherapeutic drugs 3'-azido-3'-deoxythymidine (AZT), 2',3'- dideoxycytidine (ddC) and 2'-deoxy-2',2'-difluorocytidine (gemcitabine) (PubMed:14668133).
catalytic activity: adenosine(in) + H(+)(in) = adenosine(out) + H(+)(out) (RHEA:29987)
catalytic activity: uridine(in) + H(+)(in) = uridine(out) + H(+)(out) (RHEA:29951)
catalytic activity: thymidine(in) + H(+)(in) = thymidine(out) + H(+)(out) (RHEA:29955)
catalytic activity: cytidine(in) + H(+)(in) = cytidine(out) + H(+)(out) (RHEA:29983)
catalytic activity: 2'-deoxycytidine(in) + H(+)(in) = 2'-deoxycytidine(out) + H(+)(out) (RHEA:29975) - substrates: H+, Nucleosides
- Stationary-phase quorum-sensing signals affect autoinducer-2 and gene expression in Escherichia coli
Ren, Applied and environmental microbiology 2004 - “...3 genes (Table 3) and Gene b no. Expression ratio nupC b2393 5.3 phoB b0399 4.5 phoU b3724 4.4 argT b2310 4.2 ompF b0929 3.8 phoA b0383 2.7 a Description...”
- “...E. coli stationary-phase supernatanta Expression ratio b0399 35.7 nupC b2393 8.8 phoU b3724 6.5 phoR argT b0400 b2310 5.6 5 nlpC dsdX gltB ompF guaC phoE bcr...”
- The Escherichia coli gabDTPC operon: specific gamma-aminobutyrate catabolism and nonspecific induction
Schneider, Journal of bacteriology 2002 - “...transport/catabolic operon (b1440-1444), yedL (b1932), nupC (b2393), and fklB-cycA (b4207-4208) and represses serA (b2913) and gltBDF (b32123214) (50)....”
- Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation
Zimmer, Proceedings of the National Academy of Sciences of the United States of America 2000 - “...b1483-8 b1744-8 b1783-4 b1932 b1987 b1988 b2306-9 b2310 b2393 b2661-4 b3073 b3268-71 b3540-4 b3868-70 b4207-8 Total: 25 operons; 75 genes References for control...”
- “...and dipeptides, respectively, and two operons (b2393 and b4208) encoding secondary ion-coupled transporters for nucleosides, and D-alanineD-serineglycine,...”
- Elucidation of the Gemcitabine Transporters of Escherichia coli K-12 and Gamma-Proteobacteria Linked to Gemcitabine-Related Chemoresistance
Iosifidou, International journal of molecular sciences 2024 - “...and NHS sequences. The genes mobilized correspond to the coding sequences of E. coli NupC (P0AFF2), NupX (P33021), YeiM/PsuT (P33024), NupG (P0AFF4), XapB (P45562), and YegT (P76417) (UniProt numbers given in parentheses), K. pneumoniae KpNupC (A0A2W0KM59), KpNupC2 (WP_002898911), KpvcCNT (WP_004146034), and KpNupG (WP_038806797), and C. freundii...”
- Biodistribution of 89Zr-DFO-labeled avian pathogenic Escherichia coli outer membrane vesicles by PET imaging in chickens
Li, Poultry science 2023 - “...production and conversion Cell inner membrane 332 P76576 YFGM Function unknown Cell inner membrane 333 P0AFF2 NUPC Nucleotide transport and metabolism Cell inner membrane 334 P31224 ACRB Defense mechanisms Cell inner membrane 335 P0AAA1 YAGU Function unknown Cell inner membrane 336 P07014 SDHB Energy production and...”
- A Novel and Fast Purification Method for Nucleoside Transporters
Hao, Frontiers in molecular biosciences 2016 (no snippet) - Sequential Acquisition of Virulence and Fluoroquinolone Resistance Has Shaped the Evolution of Escherichia coli ST131
Ben, mBio 2016 - “...Glutathione-regulated potassium efflux antiporter 2705332 G G G G A A A A A Syn EC958_5013 334 nupC Nucleoside-transport system protein NupC 3549826 A A A A T T T T T FY EC958_3501 184 yqjA Hypothetical protein 3587935 G G G G A A A...”
- The molecular basis for control of ETEC enterotoxin expression in response to environment and host
Haycocks, PLoS pathogens 2015 - “...yeiQ 2555083 CG TGA CCAAAG TCTCA (ETEC_2360) (yfaQ) 2729713 TT TGA AGCTTG TCACA ETEC_2510 / ETEC_2511 mntH / nupC 2735124 A GT T A TTCATG TCAC G ETEC_2514 yfeC 2795423 TGTGA GCCATGA CACA (ETEC_2572)/ETEC_2573 (aegA)/narQ 2810983 C GTGA TCAAGA TCACA ETEC_2586 hyfA 2887131 T T TGA...”
- RNA-seq analysis of the influence of anaerobiosis and FNR on Shigella flexneri
Vergara-Irigaray, BMC genomics 2014 - “...putative transmembrane subunit -3.70 2.71 Nucleotide transport and metabolism SF5M90T_3587 udp uridine phosphorylase 3.12 0.59 SF5M90T_2387 nupC permease of transport system for 3 nucleosides 2.81 SF5M90T_2949 nupG nucleoside permease 2.47 SF5M90T_674 ybeK putative tRNA synthetase 1.60 SF5M90T_444 adk adenylate kinase 1.51 -0.67 SF5M90T_2456 purC phosphoribosylaminoimidazole-succinocarboxamidesynthetase 1.35...”
c2932 Nucleoside permease nupC from Escherichia coli CFT073
28% identity, 96% coverage
VDA_002313 NupC/NupG family nucleoside CNT transporter from Photobacterium damselae subsp. damselae CIP 102761
29% identity, 93% coverage
VK055_4768 nucleoside permease NupC from Klebsiella pneumoniae subsp. pneumoniae
27% identity, 96% coverage
STM2409 NUP family, nucleoside transport from Salmonella typhimurium LT2
SL1344_2377 nucleoside permease NupC from Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344
28% identity, 93% coverage
- Selection-Driven Gene Inactivation in Salmonella
Cherry, Genome biology and evolution 2020 - “...Access publication February 11, 2020 23 STM0874 STM0578 STM2409 STM3333 STM1346 STM2859 STM4325 mdaA nfnB Other transnupC porter proteins -- ydiE Metabolic...”
- Role of sapA and yfgA in Susceptibility to Antibody-Mediated Complement-Dependent Killing and Virulence of Salmonella enterica Serovar Typhimurium
Ondari, Infection and immunity 2017 - “...495 2,892 2.330 5.29E39 Lipopolysaccharide core biosynthesis protein SL1344_3280 147 1,118 2.302 4.84E38 Hypothetical protein SL1344_2377 nupC 204 1,303 2.206 7.05E35 Nucleoside permease NupC SL1344_3927 tatA 408 2,225 2.194 1.73E34 sec-independent protein translocase protein SL1344_3726 slsA 1,288 6,222 2.187 2.89E34 Hypothetical protein SL1344_0091 ksgA 187 1,158...”
LSA0259 Pyrimidine-specific nucleoside symporter from Lactobacillus sakei subsp. sakei 23K
27% identity, 98% coverage
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...-0.9 -1.5 LSA0055 lsa0055 Putative thiamine/thiamine precursor:cation symporter 1.6 LSA0064 lsa0064 Putative nucleobase:cation symporter -0.8 LSA0259 lsa0259 Pyrimidine-specific nucleoside symporter 1.5 1.3 LSA0798* lsa0798 Pyrimidine-specific nucleoside symporter 3.5 2.2 1.7 LSA0799* lsa0799 Putative purine transport protein 4.4 2.7 2.9 LSA1210 lsa1210 Putative cytosine:cation symporter (C-terminal fragment),...”
FTN_1599 nucleoside permease NUP family protein from Francisella tularensis subsp. novicida U112
26% identity, 98% coverage
- Francisella-arthropod vector interaction and its role in patho-adaptation to infect mammals
Akimana, Frontiers in microbiology 2011 - “...FTN_1593 ABC-type oligopeptide transport system, periplasmic component oppA FTN_1595 Signal recognition particle receptor FtsY ftsY FTN_1599 Nucleoside permease NUP family protein nupC FTN_1600 Nucleoside permease NUP family protein nupC1 FTN_1608 Disulfide bond formation protein dsbB FTN_1611 Major facilitator superfamily (MFS) transport protein FTN_1612 Hypothetical protein FTN_1617...”
A0KFP6 Nucleoside permease NupC from Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049)
AHA_0539 nucleoside permease NupC from Aeromonas hydrophila subsp. hydrophila ATCC 7966
29% identity, 85% coverage
BC2973 Nucleoside permease nupC from Bacillus cereus ATCC 14579
29% identity, 98% coverage
- Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus
Caro-Astorga, NPJ biofilms and microbiomes 2020 - “...synthesis required for nucleoside synthesis (BC0403*, BC0639, BC0640*, BC2383 , BC3939*, BC2790), nucleoside transporters ( BC2973, BC0363 ) and specific phosphate transporters ( BC4265-68 and BC0711 ), all molecules required for DNA synthesis. These results suggest that these metabolic changes might be, among others, oriented to...”
SCA_0293 NupC/NupG family nucleoside CNT transporter from Staphylococcus carnosus subsp. carnosus TM300
Sca_0293 putative sodium dependent nucleoside transporter from Staphylococcus carnosus subsp. carnosus TM300
27% identity, 97% coverage
WP_002898911 NupC/NupG family nucleoside CNT transporter from Klebsiella pneumoniae
27% identity, 93% coverage
- Elucidation of the Gemcitabine Transporters of Escherichia coli K-12 and Gamma-Proteobacteria Linked to Gemcitabine-Related Chemoresistance
Iosifidou, International journal of molecular sciences 2024 - “...XapB (P45562), and YegT (P76417) (UniProt numbers given in parentheses), K. pneumoniae KpNupC (A0A2W0KM59), KpNupC2 (WP_002898911), KpvcCNT (WP_004146034), and KpNupG (WP_038806797), and C. freundii CfNupC (A0A336NW46), CfPsuT (D2TRJ2), CfNupG (A0A7D6VR53), CfXapB (A0A7W3D7V4), and CfYegT (A0A7D6VSQ9) (trEMBL or NCBI accession numbers in parentheses). Other sequences of NHS...”
YPK_1438 nucleoside transporter from Yersinia pseudotuberculosis YPIII
YPO2983 nucleoside permease from Yersinia pestis CO92
YPTB2706 CNT family H+/nucleoside symporter from Yersinia pseudotuberculosis IP 32953
27% identity, 96% coverage
- Functional versatility of Zur in metal homeostasis, motility, biofilm formation, and stress resistance in Yersinia pseudotuberculosis
Gu, Microbiology spectrum 2024 - “...maltose (YPK_0378 and YPK_0382), simple sugar (YPK_24082411), glutamate/aspartate (YPK_3010), oligopeptide (YPK_2067 and YPK_2068), and nucleoside (YPK_1438 and YPK_3628) transport systems were upregulated by Zur. This suggests Zurs role in nutrient acquisition in Y. pseudotuberculosis . Although similar regulation of amino acid and oligopeptide transport systems was...”
- The cyclic AMP receptor protein, CRP, is required for both virulence and expression of the minimal CRP regulon in Yersinia pestis biovar microtus
Zhan, Infection and immunity 2008 - “...A AAACTGTGATTTGACTC ACTCTG D/321/9.24 YPO2982 R/136/9.24 YPO2983 D/327/9.93 YPO3633 D/124/9.78 YPO3954 D/265/9.92 YPO4037 D/255/10.35 YPO1109 2 D/478/8.4...”
- Growth of Yersinia pseudotuberculosis in human plasma: impacts on virulence and metabolic gene expression
Rosso, BMC microbiology 2008 - “...YPO2013 ribose-phosphate pyrophosphokinase 0.55 (< 0.001) YPTB2102 (tdk) YPO2176 thymidine kinase 0.75 (0.034) YPTB2706 (nupC) YPO2983 nucleoside permease 1.674 (0.018) YPTB2781 (purC) YPO3059 phosphoribosylaminoimidazole-succinocarboxamide synthase (pseudogene. IS 0.598 (0.009) YPTB2794 (upp) YPO2827 uracil phosphoribosyltransferase 0.515 (0.002) YPTB2796 (purN) YPO2829 putative phosphoribosylglycinamide formyltransferase 1.842 (0.038) YPTB2803 (ppx)...”
- Growth of Yersinia pseudotuberculosis in human plasma: impacts on virulence and metabolic gene expression
Rosso, BMC microbiology 2008 - “...YPTB2001 (prsA) YPO2013 ribose-phosphate pyrophosphokinase 0.55 (< 0.001) YPTB2102 (tdk) YPO2176 thymidine kinase 0.75 (0.034) YPTB2706 (nupC) YPO2983 nucleoside permease 1.674 (0.018) YPTB2781 (purC) YPO3059 phosphoribosylaminoimidazole-succinocarboxamide synthase (pseudogene. IS 0.598 (0.009) YPTB2794 (upp) YPO2827 uracil phosphoribosyltransferase 0.515 (0.002) YPTB2796 (purN) YPO2829 putative phosphoribosylglycinamide formyltransferase 1.842 (0.038)...”
SERP0304 nucleoside permease NupC, putative from Staphylococcus epidermidis RP62A
27% identity, 97% coverage
NUPG_BACSU / P42312 Purine nucleoside transport protein NupG from Bacillus subtilis (strain 168) (see paper)
TC 2.A.41.1.3 / P42312 The purine nucleoside uptake transporter NupG (YxjA) from Bacillus subtilis (see 4 papers)
BSU39020 purine nucleoside transporter from Bacillus subtilis subsp. subtilis str. 168
27% identity, 98% coverage
- function: Involved in the uptake of the purine ribonucleosides inosine and guanosine.
- substrates: Purines
- A mobile genetic element profoundly increases heat resistance of bacterial spores
Berendsen, The ISME journal 2016 - “...the two The ISME Journal divergently transcribed genes yxjA (BSU39020) and yxjB (BSU39010) and their spores needed even longer average heating times of 8.8 min...”
- Secondary structural entropy in RNA switch (Riboswitch) identification
Manzourolajdad, BMC bioinformatics 2015 - “...-54.30 - 0.4850 124.8850021 - 0.3550 10402 rpsT BSU25550 0.8240758777 200 nt 4007404 4007603 forward BSU39020 yxjA -360 -48.97 - 0.4150 124.6660004 - 0.3950 2902 citH BSU39060 0.8239642382 200 nt 2376722 2376921 forward BSU22510 ypjC -15121 -44.40 - 0.3450 124.1429977 - 0.4000 16602 ypzI BSU22869 0.8239628077...”
- “...-69.00 - 0.5400 120.5859985 - 0.3000 402 yetO BSU07250 0.8170907497 200 nt 4007304 4007503 forward BSU39020 yxjA -260 -49.30 - 0.4200 124.4300003 - 0.3950 3002 citH BSU39060 0.8151187301 200 nt 3726352 3726551 forward BSU36160 ywqM -1918 -63.90 - 0.5250 122.0380020 - 0.3200 7402 ywqB BSU36270 0.8136813045...”
LSA0798 Pyrimidine-specific nucleoside symporter from Lactobacillus sakei subsp. sakei 23K
28% identity, 98% coverage
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...1.6 LSA0064 lsa0064 Putative nucleobase:cation symporter -0.8 LSA0259 lsa0259 Pyrimidine-specific nucleoside symporter 1.5 1.3 LSA0798* lsa0798 Pyrimidine-specific nucleoside symporter 3.5 2.2 1.7 LSA0799* lsa0799 Putative purine transport protein 4.4 2.7 2.9 LSA1210 lsa1210 Putative cytosine:cation symporter (C-terminal fragment), authentic frameshift -0.8 -0.6 LSA1211 lsa1211 Putative cytosine:cation...”
- “...aldolase, and is located in an operon structure preceding the genes deoB , deoD , lsa0798 , lsa0799 , deoR and pdp which encode phosphopentomutase, purine nucleoside phosphorylase, pyrimidine-specific nucleoside symporter, a putative purine transport protein, the deoxyribonucleoside synthesis operon transcriptional regulator (DeoR), and a pyrimidine-nucleoside...”
FTN_1600 nucleoside permease NUP family protein from Francisella tularensis subsp. novicida U112
27% identity, 98% coverage
- Francisella-arthropod vector interaction and its role in patho-adaptation to infect mammals
Akimana, Frontiers in microbiology 2011 - “...oppA FTN_1595 Signal recognition particle receptor FtsY ftsY FTN_1599 Nucleoside permease NUP family protein nupC FTN_1600 Nucleoside permease NUP family protein nupC1 FTN_1608 Disulfide bond formation protein dsbB FTN_1611 Major facilitator superfamily (MFS) transport protein FTN_1612 Hypothetical protein FTN_1617 Sensor histidine kinase qsec FTN_1618 Conserved hypothetical...”
AUC50_03495 NupC/NupG family nucleoside CNT transporter from Staphylococcus aureus
26% identity, 97% coverage
SAR0655 putative Na+ dependent nucleoside transporter from Staphylococcus aureus subsp. aureus MRSA252
26% identity, 97% coverage
SACOL0701 nucleoside permease NupC, putative from Staphylococcus aureus subsp. aureus COL
SA0600 hypothetical protein from Staphylococcus aureus subsp. aureus N315
SAUSA300_0631 putative nucleoside transporter from Staphylococcus aureus subsp. aureus USA300_FPR3757
RSAU_000600 NupC/NupG family nucleoside CNT transporter from Staphylococcus aureus subsp. aureus 6850
26% identity, 97% coverage
- Inactivation of thyA in Staphylococcus aureus attenuates virulence and has a strong impact on metabolism and virulence gene expression
Kriegeskorte, mBio 2014 - “...analysis, there was a strong upregulation of SACOL0566 ( nupC ), while the SACOL0310 and SACOL0701 genes were unaffected. To confirm the biological function of nupC as a pyrimidine transporter, which is responsible for the uptake of extracellular thymidine, we tested a nupC mutant for its...”
- Characterization of RNA Helicase CshA and Its Role in Protecting mRNAs and Small RNAs of Staphylococcus aureus Strain Newman
Kim, Infection and immunity 2016 - “...uvrC, sdhC codY, rpsB sa1590, ribD sa0372, xprT sa0543, sa0544 sa0601, sa0600 ssaA sa0751 171 150 107 118 210 1,176 420 204 252 225 126 162 116 147 238 109 118...”
- Direct targets of CodY in Staphylococcus aureus
Majerczyk, Journal of bacteriology 2010 - “...ilvD ilvB SA2044 SA2045 SA2046 SA2047 SA2048 SA2049 SA2050 SA0600 ilvN ilvC leuA leuB leuC leuD ilvA ilvE Methionine SA0431 SA0430 SA0429 SA0428 ilvE metE...”
- Transcriptional profiling reveals that daptomycin induces the Staphylococcus aureus cell wall stress stimulon and genes responsive to membrane depolarization
Muthaiyan, Antimicrobial agents and chemotherapy 2008 - “...University of California, Berkeley SA1363 SA1431 SA1364 SA0600 Protein 984 MUTHAIYAN ET AL. ANTIMICROB. AGENTS CHEMOTHER. TABLE 1--Continued Category and locusb...”
- Overexpression of genes of the cell wall stimulon in clinical isolates of Staphylococcus aureus exhibiting vancomycin-intermediate- S. aureus-type resistance to vancomycin
McAleese, Journal of bacteriology 2006 - “...SA2253 SA2254 SA0242 SA1718 SA1970 SA0022 SA0131 SA0479 SA0600 SA1041 SA1042 SA0298 SA0325 SA1992 SA1993 SA1994 SA1995 SA1996 SA1997 SA2241 SA2294 SA0204 SA0241...”
- Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Genetic Factors Involved in Human Endothelial Cells Damage, an Important Phenotype Correlated with Persistent Endovascular Infection
Xiao, Antibiotics (Basel, Switzerland) 2022 - “...hypothetical phiSLT ORF412-like protein, portal protein 17.28 10.80 SAUSA300_2432 hypothetical MutT/NUDIX family hydrolase 17.26 15.82 SAUSA300_0631 hypothetical putative nucleoside transporter 17.25 11.20 SAUSA300_1000 potB spermidine/putrescine ABC transporter permease 17.14 5.86 SAUSA300_2559 hypothetical DNA-binding response regulator 17.10 8.85 SAUSA300_2467 srtA sortase 17.01 6.72 SAUSA300_2300 hypothetical transcriptional regulator,...”
- “...12.24 22.52 a SAUSA300_0649 hypothetical 20.24 0.89 22.65 a SAUSA300_2587 hypothetical 20.06 9.42 26.45 a SAUSA300_0631 hypothetical 17.25 11.20 23.00 a SAUSA300_2027 alr 16.70 16.05 3.28 1.38 SAUSA300_2055 murA 15.79 10.49 7.62 0.59 SAUSA300_1682 ccpA 14.04 8.43 13.43 a SAUSA300_1696 dat 12.74 5.48 14.99 1.34 SAUSA300_0974...”
- Identification of a Novel LysR-Type Transcriptional Regulator in Staphylococcus aureus That Is Crucial for Secondary Tissue Colonization during Metastatic Bloodstream Infection
Groma, mBio 2020 - “...5.22 1.50E04 Transporter anion-sodium symporter family protein RSAU_002543 5.25 1.24E04 nixA RSAU_001219 5.28 2.50E04 guaC RSAU_000600 5.31 7.74E04 nupC2 RSAU_002444 5.35 1.17E04 Amino acid permease family protein RSAU_000994 5.58 4.08E07 typA RSAU_000042 5.59 2.28E04 Dienelactone hydrolase family protein RSAU_002289 5.60 1.96E04 rpsP RSAU_001080 5.62 7.42E04 rluD...”
lp_3204 nucleoside transport protein from Lactobacillus plantarum WCFS1
25% identity, 97% coverage
XP_011249177 sodium/nucleoside cotransporter 1 isoform X2 from Mus musculus
31% identity, 38% coverage
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory