PaperBLAST
PaperBLAST Hits for WP_043847943.1 glycosyltransferase (Amycolatopsis keratiniphila) (408 a.a., MRVLLSTCGS...)
Show query sequence
>WP_043847943.1 glycosyltransferase (Amycolatopsis keratiniphila)
MRVLLSTCGSRGDVEPLVALAVRLRERGAEVRMCAPPDCADRLAEVDVPHLPLGASARPS
AGQAKPLTAEDMLRFTTETIATQFERIPAAAEGCAAVVTTGLLAAAIGVRSVAEKLGIPY
FYGFHCPSYVPSPYYAPPPPLGEPPAPDGTDIQALWERNNQSAYRRYGEPLNSRRAAIGL
PPVEDIFGHGYTDHPWMAADPVLAPLQPTDLDAVQTGAWILPDERPISAELEAFLDAGAP
PVYLGFGSLRAPADAAKVAIEAIRAHGHRVILSRGWADLVLPDDREDCFAIGEVNQQVLF
RRVAAVIHHGGAGTTHVATRAGVPQILVPQIADQPYYAARVAELGVGVAHDGPTPTFDTL
SAALTKALAPETRVRAEAVAETVQTDGAAVAADLLFAAVTGNQPAVPA
Running BLASTp...
Found 183 similar proteins in the literature:
AAB49299.1 TDP/UDP-Glc: aglycosyl-vancomycin glucosyltransferase (GtfE';Vcm10) (EC 2.4.1.-) (see protein)
AORI_1487 glycosyltransferase from Amycolatopsis keratiniphila
100% identity, 100% coverage
- Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis
Xu, BMC genomics 2014 - “...7 , 8 ], only two glycosyltransferases are encoded in the vcm cluster (AORI_1486 and AORI_1487). On the other hand, the vancomycin-resistance genes vanHAX (AORI_1471-AORI_1473) are only predicted in vcm and not in the other two clusters (Table 2 ). Throughout the A. orientalis genome, we...”
- “...Cytochrome P450 lcl|AJ223998.1_cdsid_CAA11791.1 91.69 AORI_1485 vhal Halogenase lcl|Y16952.3_cdsid_CAA76550.1 93.89 AORI_1486 gtfD Vancosaminyl transferase lcl|Y16952.3_cdsid_CAA76553.1 69.93 AORI_1487 gtfE Glycosyl transferase lcl|Y16952.3_cdsid_CAA76552.1 81.17 AORI_1488 vcaC Methyltransferase family protein lcl|Y16952.3_cdsid_CAC48364.1 93.87 AORI_1489 LmbE family protein lcl|Y16952.3_cdsid_CAC48365.1 75.91 AORI_1490 vmt Methyltransferase lcl|AJ223998.1_cdsid_CAA11779.1 73.21 AORI_1491 hpgT 4-hydroxyphenylglycine aminotransferase lcl|AJ223998.1_cdsid_CAA11790.1 89.7 AORI_1492...”
GTFE_AMYOR / Q9AFC6 Glycosyltransferase GtfE; EC 2.4.1.- from Amycolatopsis orientalis (Nocardia orientalis) (see 2 papers)
AAK31353.1 TDB/UDP-Glc: aglycosyl-vancomycin glucosyltransferase (GtfE) (EC 2.4.1.-) (see protein)
90% identity, 100% coverage
- function: D-glucosyltransferase that acts on the aglycone core, transferring D-glucose to the phenolic hydroxyl of OH-Phegly(4) to form a devancoaminyl-vancomycin (DVV) intermediate in the biosynthesis of glycopeptide antibiotic vancomycin. Also able to glycosylate A47934, an antibiotic with a teicoplanin-like heptapeptide, but lacking sugar residues.
GTFB_AMYOR / P96559 Vancomycin aglycone glucosyltransferase; Glycosyltransferase GtfB; EC 2.4.1.310 from Amycolatopsis orientalis (Nocardia orientalis) (see 4 papers)
P96559 vancomycin aglycone glucosyltransferase (EC 2.4.1.310) from Amycolatopsis orientalis (see 2 papers)
AAB49293.1 TDP/UDP-Glc: aglycosyl-vancomycin: glucosyltransferase (GtfB;PCZA361.20) (EC 2.4.1.-) (see protein)
81% identity, 100% coverage
- function: Glucosyltransferase that transfers glucose to the 4-OH- Phegly(4) residue of vancomycin aglycone (AGV) to produce devancoaminyl-vancomycin (DVV) in the biosynthesis of glycopeptide antibiotic chloroeremomycin, a member of the vancomycin group of antibiotics.
catalytic activity: vancomycin aglycone + UDP-alpha-D-glucose = devancoaminyl- vancomycin + UDP (RHEA:38587)
CAA76552.1 UDP-Glc: balhimycin aglycone Hpg-glucosyltransferase B (BgtfB) (EC 2.4.1.-) (see protein)
82% identity, 100% coverage
CAE53364.1 UDP-GlcNAc: teicoplanin aglycone 4Hpg-N-acetylglucosaminidase (Tcp23;Orf10*;GtfB) (EC 2.4.1.-) (see protein)
tcp23 / CAE53364.1 GtfB protein from Actinoplanes teichomyceticus (see paper)
66% identity, 100% coverage
DMB42_RS42780 glycosyltransferase from Nonomuraea sp. WAC 01424
67% identity, 98% coverage
- Genomic-Led Discovery of a Novel Glycopeptide Antibiotic by Nonomuraea coxensis DSM 45129
Yushchuk, ACS chemical biology 2021 - “...(20) (78%) cross-linking oxygenase (OxyE) noc24 dbv10 (94.1%) DMB42_RS42790 (21) (87%) halogenase noc25 dbv9 (90.4%) DMB42_RS42780 (23) (74%) glycosyltransferase (GtfB) noc26 dbv8 (87.5%) DMB42_RS42775 (24) (77%) acyltransferase noc27 dbv7 (87.3%) DMB42_RS42865 (6) (78%) VanY-carboxypeptidase noc28 dbv6 (95.9%) DMB42_RS42855 (8) (92%) response regulator noc29 dbv5 (92.8%) DMB42_RS42860...”
GTFC_AMYOR / P96560 Glycosyltransferase GtfC; EC 2.4.1.- from Amycolatopsis orientalis (Nocardia orientalis) (see 3 papers)
AAB49294.1 UDP-β-L-4-epi-vancosamine: vancomycin-pseudoaglycone vancosaminyltransferase (GtfC;PCZA361.21) (EC 2.4.1.-) (see protein)
63% identity, 99% coverage
- function: Catalyzes the attachment of dTDP-L-4-epi-vancosamine to chloroorienticin B to form chloroeremomycin in the biosynthesis of glycopeptide antibiotic chloroeremomycin, a member of the vancomycin group of antibiotics. Also able to use dTDP-L-4-epi-vancosamine and devancoaminyl-vancomycin (DVV) to create epivancomycin. Acts downstream of GtfA.
catalytic activity: dTDP-beta-L-vancosamine + devancoaminyl-vancomycin = epivancomycin + dTDP + H(+) (RHEA:45904)
catalytic activity: chloroorienticin B + dTDP-beta-L-vancosamine = chloroeremomycin + dTDP + H(+) (RHEA:45908)
AAB49298.1 UDP-β-L-4-epi-vancosamine: vancomycin-pseudoaglycone vancosaminyltransferase (GtfD';Vcm9) (EC 2.4.1.-) (see protein)
AORI_1486 glycosyltransferase from Amycolatopsis keratiniphila
65% identity, 100% coverage
- Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis
Xu, BMC genomics 2014 - “...predicted [ 7 , 8 ], only two glycosyltransferases are encoded in the vcm cluster (AORI_1486 and AORI_1487). On the other hand, the vancomycin-resistance genes vanHAX (AORI_1471-AORI_1473) are only predicted in vcm and not in the other two clusters (Table 2 ). Throughout the A. orientalis...”
- “...Cytochrome P450 lcl|Y16952.3_cdsid_CAA76548.1 87.44 AORI_1484 oxyC Cytochrome P450 lcl|AJ223998.1_cdsid_CAA11791.1 91.69 AORI_1485 vhal Halogenase lcl|Y16952.3_cdsid_CAA76550.1 93.89 AORI_1486 gtfD Vancosaminyl transferase lcl|Y16952.3_cdsid_CAA76553.1 69.93 AORI_1487 gtfE Glycosyl transferase lcl|Y16952.3_cdsid_CAA76552.1 81.17 AORI_1488 vcaC Methyltransferase family protein lcl|Y16952.3_cdsid_CAC48364.1 93.87 AORI_1489 LmbE family protein lcl|Y16952.3_cdsid_CAC48365.1 75.91 AORI_1490 vmt Methyltransferase lcl|AJ223998.1_cdsid_CAA11779.1 73.21 AORI_1491...”
1rrvA / Q9AFC7 X-ray crystal structure of tdp-vancosaminyltransferase gtfd as a complex with tdp and the natural substrate, desvancosaminyl vancomycin. (see paper)
64% identity, 95% coverage
- Ligands: peptide; thymidine-5'-diphosphate; beta-d-glucopyranose (1rrvA)
gtfD / Q9AFC7 desvancosaminyl-vancomycin vancosaminetransferase (EC 2.4.1.322) from Amycolatopsis orientalis (see 2 papers)
GTFD_AMYOR / Q9AFC7 Devancosaminyl-vancomycin vancosaminetransferase; Devancosamine-vancomycin TDP-vancosaminyltransferase; Glycosyltransferase GtfD; EC 2.4.1.322 from Amycolatopsis orientalis (Nocardia orientalis) (see 2 papers)
Q9AFC7 devancosaminyl-vancomycin vancosaminetransferase (EC 2.4.1.322) from Amycolatopsis orientalis (see 5 papers)
AAK31352.1 UDP-β-L-4-epi-vancosamine: vancomycin-pseudoaglycone vancosaminyltransferase (GtfD) (EC 2.4.1.-) (see protein)
64% identity, 100% coverage
- function: Catalyzes the attachment of L-vancosamine to a monoglucosylated heptapeptide intermediate during the final stage of glycopeptide antibiotic vancomycin biosynthesis.
catalytic activity: dTDP-beta-L-vancosamine + devancoaminyl-vancomycin = vancomycin + dTDP + H(+) (RHEA:39891)
CAE53349.1 UDP-GlcNAc: teicoplanin aglycone 3-Cl-6-β-Hty-N-acetylglucosaminidase (Tcp8;Orf1;GtfA) (EC 2.4.1.-) (see protein)
tcp8 / CAE53349.1 GtfA protein from Actinoplanes teichomyceticus (see paper)
61% identity, 100% coverage
3h4iA / P96558,Q6ZZJ7 Chimeric glycosyltransferase for the generation of novel natural products (see paper)
60% identity, 98% coverage
- Ligand: uridine-5'-diphosphate-2-deoxy-2-fluoro-alpha-d-glucose (3h4iA)
GTFA_AMYOR / P96558 dTDP-epi-vancosaminyltransferase; Glycosyltransferase AH1; GtfAH1; Glycosyltransferase GtfA; EC 2.4.1.311 from Amycolatopsis orientalis (Nocardia orientalis) (see 4 papers)
P96558 chloroorienticin B synthase (EC 2.4.1.311) from Amycolatopsis orientalis (see 2 papers)
AAB49292.1 dTDP-β-L-4-epi-epivancosamine: epivancosaminyltransferase (GtfA;PCZA361.19) (EC 2.4.1.-) (see protein)
59% identity, 100% coverage
- function: Catalyzes the attachment of 4-epi-vancosamine from a TDP donor to the beta-OH-Tyr-6 of the aglycone cosubstrate in the biosynthesis of glycopeptide antibiotic chloroeremomycin, a member of the vancomycin group of antibiotics. Strongly prefers devancoaminyl- vancomycin (DVV) as substrate rather than the heptapeptide vancomycin aglycone (AGV). Acts downstream of GtfB.
catalytic activity: dTDP-beta-L-4-epi-vancosamine + devancoaminyl-vancomycin = chloroorienticin B + dTDP + H(+) (RHEA:38591) - Conserved Conformational Hierarchy across Functionally Divergent Glycosyltransferases of the GT-B Structural Superfamily as Determined from Microsecond Molecular Dynamics.
Ramirez-Mondragon, International journal of molecular sciences 2021 - “...Alignment (MSA) The MSA was generated by filtering the HepI (UniProtKB: P24173) and GtfA (UniProtKB: P96558) sequences on the European Bioinformatics Institutes (EMBLEBI) Clustal Omega MSA web server with default settings. The resulting MSA was visualized and sequence identity conservation was determined using the Unipro UGENE...”
1pn3A / P96558 Crystal structure of tdp-epi-vancosaminyltransferase gtfa in complexes with tdp and the acceptor substrate dvv. (see paper)
60% identity, 98% coverage
- Ligands: peptide; thymidine-5'-diphosphate; beta-d-glucopyranose (1pn3A)
ADN68481.1 UDP-Glc: sorangicin A glucosyltransferase (SorF) (EC 2.4.1.-) (see protein)
42% identity, 94% coverage
XALc_0365 putative glucosyltransferase protein from Xanthomonas albilineans
32% identity, 88% coverage
K4BTE6 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Solanum lycopersicum (see paper)
29% identity, 63% coverage
SGTL1 / Q2I015 3β-hydroxy sterol glucosyltransferase (EC 2.4.1.173) from Withania somnifera (see 4 papers)
29% identity, 56% coverage
A0A023NFQ4 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Gossypium hirsutum (see paper)
29% identity, 60% coverage
WP_012855060 glycosyltransferase from Thermomonospora curvata DSM 43183
32% identity, 96% coverage
LOC115709176 sterol 3-beta-glucosyltransferase UGT80B1 from Cannabis sativa
27% identity, 61% coverage
WP_030163570 glycosyltransferase from Spirillospora albida
33% identity, 84% coverage
AT3G07020 UDP-glucose:sterol glucosyltransferase (UGT80A2) from Arabidopsis thaliana
NP_850529 UDP-Glycosyltransferase superfamily protein from Arabidopsis thaliana
28% identity, 62% coverage
- Leaf pigmentation in Cannabis sativa: Characterization of anthocyanin biosynthesis in colorful Cannabis varieties
Gagalova, Plant direct 2024 - “...superfamily 45 64 LOC115709176 XM030637224 XP030493084 640 AT1G43620 UGT80B1 82 90 LOC115712970 XM030641414 XP030497274 627 AT3G07020 UGT80B2 79 87 LOC115720702 XM030649869 XP030505729 606 AT3G07020 UGT80B2 74 85 LOC115720702 XM030649870 XP030505730 605 AT3G07020 UGT80B2 74 85 LOC115703173 XM030630692 XP030486552 488 AT4G01070 UGT72B1 44 62 LOC115705536 XM030632889 XP030488749...”
- Genome-Wide Association Study of Lint Percentage in Gossypium hirsutum L. Races
Wang, International journal of molecular sciences 2023 - “...myo-inositol-1-phosphate synthase gene family) [ 42 ]; WD40 [ 15 ]; Gh_D08G2376 (a homolog of AT3G07020 and GhSGT1 ) [ 17 ]; Gh_A02G1392 ( AIL6 , a homolog of the AP2/ETHYLENE RESPONSE FACTOR (ERF)-type transcription-factor-encoding gene AINTEGUMENTA-like 6 in Arabidopsis) and Gh_D08G0312 ( EIL , encoding...”
- Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana
Shiva, Plants (Basel, Switzerland) 2020 - “...UDP-glucose by a UDP-glucose:sterol glucosyltransferase (UGT). UGT80B1 (product of At1g43620 ) and UGT80A2 (product of At3g07020 ) catalyze the formation of over 85% of the sterol glucosides in Arabidopsis leaves [ 46 ]. Addition of a fatty acid to the 6-position on the glucose of a...”
- Exploring the Phytochemical Landscape of the Early-Diverging Flowering Plant Amborella trichopoda Baill
Wu, Molecules (Basel, Switzerland) 2019 - “...(AT1G24100), At UGT75B1 (AT1G05560), At UGT76B1 (AT3G11340), At UGT78D1 (AT1G30530), At UGT79B1 (AT5G54060), At UGT80A2 (AT3G07020), At UGT81A1 (AT4G31780), At UGT82A1 (AT3G22250), At UGT83A1 (AT3G02100), At UGT84A1 (AT4G15480), At UGT85A1 (AT1G22400), At UGT86A1 (AT2G36970), At UGT87A1 (AT2G30150), At UGT88A1 (AT3G16520), At UGT89A1 (AT1G51210), At UGT90A1 (AT2G16890),...”
- Effective genome editing and identification of a regiospecific gallic acid 4-O-glycosyltransferase in pomegranate (Punica granatum L.)
Chang, Horticulture research 2019 - “...(AT2G26480), At UGT76E1 (AT5G59580), At UGT78D1 (AT1G30530), At UGT79B6 (AT5G54010), At UGT80B1 (AT1G43620), At UGT80A2 (AT3G07020), AtUGT81A1 (AT4G31780), At UGT82A1 (AT3G22250), At UGT83A1 (AT3G02100), At UGT84B1 (AT2G23260), At UGT85A1 (AT1G22400), At UGT86A1 (AT2G36970), At UGT87A1 (AT2G30150), At UGT88A1 (AT3G16520), At UGT89A2 (AT5G03490), At UGT89B1 (AT1G73880), At...”
- Population structure and genetic basis of the agronomic traits of upland cotton in China revealed by a genome-wide association study using high-density SNPs
Huang, Plant biotechnology journal 2017 - “...S14 ). Although there is no function information for Gh_D08G2376 in cotton, its homologous genes AT3G07020 and GhSGT1 have clear function information. AT3G07020 ( UGT80A2 ) mutant plants have lower steryl glycoside and acyl steryl glycoside levels and reduced seed size in Arabidopsis (DeBolt etal .,...”
- Genome-wide analysis of UDP-glycosyltransferase super family in Brassica rapa and Brassica oleracea reveals its evolutionary history and functional characterization
Yu, BMC genomics 2017 - “...aquatic plants. The UGT80 gene family has two members in A. thaliana , including UGT80A2 (AT3G07020) and UGT80B1 (AT1G43620). UGT80A2 was investigated to be required for normal levels of major steryl glycosides in seeds, whereas UGT80B1 is involved in accumulation of minor steryl glycosides (SG) and...”
- Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis
Stucky, Journal of experimental botany 2015 - “...numbers for the protein sequences in Fig. 1 . AGI locus identifiers: UGT80B1, At1g43620; UGT80A2, At3g07020; UGT713B1, At5g24750; GCS, At2g19880). The UGT nomenclature follows that previously defined by the UGT Nomenclature Committee ( Mackenzie et al. , 1997 ). Fig. 1. Neighbor-joining phylogenetic tree of UGT80-...”
- More
- Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis.
Stucky, Journal of experimental botany 2015 - GeneRIF: Both UGT80A2 and UGT80B1, but not UGT713B1 were shown to be coordinately down-regulated during seed imbibition when SG levels decline, consistent with similar functions as UGT80 enzymes.
- Mutations in UDP-Glucose:sterol glucosyltransferase in Arabidopsis cause transparent testa phenotype and suberization defect in seeds.
DeBolt, Plant physiology 2009 - GeneRIF: Arabidopsis lines having mutations in UGT80A2, UGT80B1, or both UGT80A2 and UGT80B1 were identified and characterized.
AAO95505.1 At3g07020/F17A9.17 (UDP-Glc: sterol glucosyltransferase;Sgt;Ugt80A2) (EC 2.4.1.173) (see protein)
ugt80A2 / CAB06082.1 UDP-glucose:sterol glucosyltransferase from Arabidopsis thaliana (see paper)
28% identity, 62% coverage
UGT80A2 / Q9M8Z7 3β-hydroxy sterol glucosyltransferase (EC 2.4.1.173) from Arabidopsis thaliana (see 3 papers)
U80A2_ARATH / Q9M8Z7 Sterol 3-beta-glucosyltransferase UGT80A2; UDP-glucose:sterol glucosyltransferase 80A2; EC 2.4.1.173 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9M8Z7 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Arabidopsis thaliana (see 4 papers)
NP_566297 UDP-Glycosyltransferase superfamily protein from Arabidopsis thaliana
28% identity, 62% coverage
- function: Involved in the biosynthesis of sterol glucosides. Catalyzes the synthesis of steryl glycosides (SGs) and acyl steryl glycosides (ASGs) which are the most abundant sterol derivatives in higher plants. Can act on several sterols like sitosterol, campesterol and stigmasterol. Both UGT80A2 and UGT80B1 are required for the normal production of SGs and ASGs in seeds.
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
disruption phenotype: Reduced growth rates. - The interaction networks of small rubber particle proteins in the latex of Taraxacum koksaghyz reveal diverse functions in stress responses and secondary metabolism
Wolters, Frontiers in plant science 2024 - “...AtUGT72E2, Arabidopsis thaliana UDP-glycosyltransferase superfamily protein UGT72E2 (NP_201470.1); AtUGT80A2, A. thaliana sterol 3- -glucosyltransferase UGT80A2 (NP_566297); AtUGT80B1, A. thaliana sterol 3- -glucosyltransferase UGT80B1 (NP_175027); AtUGT84A1, A. thaliana UDP-glycosyltransferase 84A1 (NP_193283.2); AtUGT713B1, A. thaliana glycosyltransferase UGT713B1 (NP_568452); CcUGT80B1, Cynara cardunculus var. scolymus sterol 3--glucosyltransferase UGT80B1 (XP_024976598.1); GhSGT1...”
- Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis
Stucky, Journal of experimental botany 2015 - “...data from this article can be found in the EMBL/GenBank data libraries under accession numbers NP_566297 ( UGT80A2 ), NP_175027 ( UGT80B1 ), NP_568452 (Col-0 UGT713B1 ), KJ396595 (Ler UGT713B1 ) and NM_127546 ( GCS ). Supplementary Table S4 lists the accession numbers for the protein...”
- Involvement of a universal amino acid synthesis impediment in cytoplasmic male sterility in pepper
Fang, Scientific reports 2016 - “...O64688 Pyruvate dehydrogenase E1 component subunit beta-3 112.11 2 406 5.03 36.81 1 0.360.06 0.480.05 Q9M8Z7 Sterol 3-beta-glucosyltransferase UGT80A2 91.22 2 637 6.27 69.28 1 0.300.04 0.780.05 Q9FHH2 101 kDa heat shock protein; HSP101-like protein 116.21 3 990 8.32 108.71 1 0.720.06 0.960.17 Q9SAK5 Myb family...”
Q6U848 Rhamnosyltransferase A (Fragment) from Mycobacterium intracellulare
27% identity, 92% coverage
U80B1_ARATH / Q9XIG1 Sterol 3-beta-glucosyltransferase UGT80B1; Protein TRANSPARENT TESTA 15; UDP-glucose:sterol glucosyltransferase 80B1; EC 2.4.1.173 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
Q9XIG1 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Arabidopsis thaliana (see 3 papers)
NP_001077674 UDP-Glycosyltransferase superfamily protein from Arabidopsis thaliana
NP_175027 UDP-Glycosyltransferase superfamily protein from Arabidopsis thaliana
AT1G43620 UDP-glucose:sterol glucosyltransferase, putative from Arabidopsis thaliana
27% identity, 64% coverage
- function: Involved in the biosynthesis of sterol glucosides. Catalyzes the synthesis of steryl glycosides (SGs) and acyl steryl glycosides (ASGs) which are the most abundant sterol derivatives in higher plants. Can act on several sterols like sitosterol, campesterol and stigmasterol. Is required for embryonic development, seed suberin accumulation, cutin formation and flavanoid accumulation in the seed coat. Both UGT80A2 and UGT80B1 are required for the normal production of SGs and ASGs in seeds.
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
disruption phenotype: Reduced growth rates. Altered embryonic development, seed suberin accumulation, cutin formation in the seed coat and reduced seed size. Lack of flavanoid accumulation in the seed coat (transparent testa phenotype). - Enfumafungin synthase represents a novel lineage of fungal triterpene cyclases
Kuhnert, Environmental microbiology 2018 - “...to a terpene cyclase. The clade groups with a characterized sterol 3--glucosyltransferase from Arabidopsis thaliana (Q9XIG1) ( DeBolt et al ., 2009 ), but the clade lacks bootstrap support. Furthermore, the enfumafungin-type GTs are nested between a cluster containing a functionally characterized sterol GT of Saccharomyces...”
- TRANSPARENT TESTA 16 and 15 act through different mechanisms to control proanthocyanidin accumulation in Arabidopsis testa.
Xu, Journal of experimental botany 2017 - GeneRIF: the roles and mode of action of TT16 and TT15 during seed development and proanthocyanidin biosynthesis
- Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis.
Stucky, Journal of experimental botany 2015 - GeneRIF: Both UGT80A2 and UGT80B1, but not UGT713B1 were shown to be coordinately down-regulated during seed imbibition when SG levels decline, consistent with similar functions as UGT80 enzymes.
- Characterization of Arabidopsis sterol glycosyltransferase TTG15/UGT80B1 role during freeze and heat stress.
Mishra, Plant signaling & behavior 2015 - GeneRIF: the functional role of TTG15/UGT80B1 gene of Arabidopsis thaliana in freeze/thaw and heat shock stress, was analyzed.
- Mutations in UDP-Glucose:sterol glucosyltransferase in Arabidopsis cause transparent testa phenotype and suberization defect in seeds.
DeBolt, Plant physiology 2009 - GeneRIF: Mutation of UGT80B1 principally alters embryonic development and seed suberin accumulation and cutin formation in the seed coat, leading to abnormal permeability and tetrazolium salt uptake. [UGT80B1]
- The interaction networks of small rubber particle proteins in the latex of Taraxacum koksaghyz reveal diverse functions in stress responses and secondary metabolism
Wolters, Frontiers in plant science 2024 - “...AtUGT80A2, A. thaliana sterol 3- -glucosyltransferase UGT80A2 (NP_566297); AtUGT80B1, A. thaliana sterol 3- -glucosyltransferase UGT80B1 (NP_175027); AtUGT84A1, A. thaliana UDP-glycosyltransferase 84A1 (NP_193283.2); AtUGT713B1, A. thaliana glycosyltransferase UGT713B1 (NP_568452); CcUGT80B1, Cynara cardunculus var. scolymus sterol 3--glucosyltransferase UGT80B1 (XP_024976598.1); GhSGT1 B-like, Gossypium hirsutum sterol glucosyltransferase 1 homolog B-like...”
- Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis
Stucky, Journal of experimental botany 2015 - “...can be found in the EMBL/GenBank data libraries under accession numbers NP_566297 ( UGT80A2 ), NP_175027 ( UGT80B1 ), NP_568452 (Col-0 UGT713B1 ), KJ396595 (Ler UGT713B1 ) and NM_127546 ( GCS ). Supplementary Table S4 lists the accession numbers for the protein sequences in Fig. 1...”
- Leaf pigmentation in Cannabis sativa: Characterization of anthocyanin biosynthesis in colorful Cannabis varieties
Gagalova, Plant direct 2024 - “...43 62 LOC115724966 XM030654353 XP030510213 466 AT2G36970 UGT superfamily 45 64 LOC115709176 XM030637224 XP030493084 640 AT1G43620 UGT80B1 82 90 LOC115712970 XM030641414 XP030497274 627 AT3G07020 UGT80B2 79 87 LOC115720702 XM030649869 XP030505729 606 AT3G07020 UGT80B2 74 85 LOC115720702 XM030649870 XP030505730 605 AT3G07020 UGT80B2 74 85 LOC115703173 XM030630692 XP030486552...”
- The APETALA2-MYBL2 module represses proanthocyanidin biosynthesis by affecting formation of the MBW complex in seeds of Arabidopsis thaliana
Jiang, Plant communications 2024 - “...TT8 (At4G09820), GL3 (At5G41315), EGL3 (At1G63650), TTG1 (At5G24520), MYBL2 (At1G71030), TTG2 (At2G37260), TT1 (At1G34790), TT15 (At1G43620), TT9 (At3G28430), TT16 (At5G23260), and STK (At4G09960). Funding This work was supported by grants from the 10.13039/501100001809 National Natural Science Foundation of China (31870281to W.J.) and the 10.13039/501100012421 Agricultural Science...”
- KIPEs3: Automatic annotation of biosynthesis pathways
Rempel, PloS one 2023 - “...and UGT79B3) A3RT* AT4G27560 [ 72 ] UGT72L1 UGT72L1* XP_013443944.2 [ 25 ] UGT80B1/TT15 TT15* AT1G43620 [ 26 ] anthocyanidin 3-O-glucoside coumaroyl CoA transferase 3AT* AT1G03940 [ 22 ] anthocyanidin 5-glucoside malonyl CoA acyl transferase 5MAT* AT3G29590 [ 22 ] anthocyanidin 3-glucoside malonyl CoA acyl transferase...”
- Shotgun proteomics of peach fruit reveals major metabolic pathways associated to ripening
Nilo-Poyanco, BMC genomics 2021 - “...0.772 1 Up_O2 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase / MECDP-synthase AT1G63970 Prupe.8G032700 0.607 1 Up_O2 Sterol 3-beta-glucosyltransferase AT1G43620 Prupe.3G097700 0.5 1 Up_O2 cinnamoyl-CoA reductase (CCR) AT1G15950 Vacuolar Sorting Prupe.7G171800 0.5 1 Up_O2 VACUOLAR SORTING PROTEIN 35 AT2G17790 Prupe.8G143700 0.607 1 Up_O2 Vacuolar-sorting receptor 3 AT2G14740 a Qualit Qualitative...”
- Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana
Shiva, Plants (Basel, Switzerland) 2020 - “...is transferred to a sterol from UDP-glucose by a UDP-glucose:sterol glucosyltransferase (UGT). UGT80B1 (product of At1g43620 ) and UGT80A2 (product of At3g07020 ) catalyze the formation of over 85% of the sterol glucosides in Arabidopsis leaves [ 46 ]. Addition of a fatty acid to the...”
- Automated Methods Enable Direct Computation on Phenotypic Descriptions for Novel Candidate Gene Prediction
Braun, Frontiers in plant science 2019 - “...(AHA) At1g17260 Enzyme ATP-ase TRANSPARENT TESTA 10 (TT10) At5g48100 Enzyme laccase TRANSPARENT TESTA 5 (TT15) At1g43620 Enzyme 3 - hydroxy sterol glucosyltransferase TRANSPARENT TESTA 11 (TT12) At3g59030 Transporter MATEefflux proton antiporter TRANSPARENT TESTA 16 (TT16) At5g23260 T F K-box, MADS-box TRANSPARENT TESTA 1 (TT1) At1g34790 T...”
- Transcript and metabolite changes during the early phase of abscisic acid-mediated induction of crassulacean acid metabolism in Talinum triangulare
Maleckova, Journal of experimental botany 2019 - “...metabolism KMT07128 AT1G69830 AMY3 1.08 6 Chromatin organisation KMT10399 AT4G00990 0.62 7 Lipid metabolism KMT19551 AT1G43620 UGT80B1 0.48 6 Nucleotide metabolism KMT00186 AT1G29900 CARB 1.06 5 Protein degradation KMT12139 AT3G61180 1.28 5 Protein modification KMT01924 AT1G28480 GRX480 2.88 5 Secondary metabolism KMS96359 AT2G20340 AAS 4.15 5...”
- Effective genome editing and identification of a regiospecific gallic acid 4-O-glycosyltransferase in pomegranate (Punica granatum L.)
Chang, Horticulture research 2019 - “...(AT5G05870), At UGT76D1 (AT2G26480), At UGT76E1 (AT5G59580), At UGT78D1 (AT1G30530), At UGT79B6 (AT5G54010), At UGT80B1 (AT1G43620), At UGT80A2 (AT3G07020), AtUGT81A1 (AT4G31780), At UGT82A1 (AT3G22250), At UGT83A1 (AT3G02100), At UGT84B1 (AT2G23260), At UGT85A1 (AT1G22400), At UGT86A1 (AT2G36970), At UGT87A1 (AT2G30150), At UGT88A1 (AT3G16520), At UGT89A2 (AT5G03490), At...”
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BAC22616.1 UDP-Glc: sterol 3-O-glucosyltransferase (PGGT-1) (EC 2.4.1.173) (see protein)
28% identity, 65% coverage
A0A023NGA8 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Gossypium hirsutum (see paper)
27% identity, 65% coverage
MAP_RS19275 glycosyltransferase from Mycobacterium avium subsp. paratuberculosis K-10
MAP3762c hypothetical protein from Mycobacterium avium subsp. paratuberculosis str. k10
31% identity, 98% coverage
ML2348 putative glycosyl transferase from Mycobacterium leprae TN
29% identity, 96% coverage
- Mycobacterium leprae's Infective Capacity Is Associated with Activation of Genes Involved in PGL-I Biosynthesis in a Schwann Cells Infection Model
Chavarro-Portillo, International journal of molecular sciences 2023 - “...( Figure 5 c). As observed for the ppsC gene, the transcriptional level of the ML2348 (coding for glucosyltransferase), ML2346c, and ML2347 (coding for methyltransferases) genes was upregulated in non-recurrent events, which could be partially due to those genes being part of the same operon at...”
- “...PGL-I ( Figure 5 b), were intriguingly compared with genes at locus 1 (ML2346c, ML2347, ML2348). Gene transcription of ML0126 and ML0127 was activated in recurrent M. leprae , specifically ML0126 between Days 0 and 7 post-infection ( Figure 5 h,i). For the non-recurrent M. leprae...”
- PGL-III, a Rare Intermediate of Mycobacterium leprae Phenolic Glycolipid Biosynthesis, Is a Potent Mincle Ligand
Ishizuka, ACS central science 2023 - “...2- O -methyltransferase (ML0127), rhamnosyl transferase (ML0128), two glucosyl methyltransferases (ML2346 and ML2347), and glucosyltransferase (ML2348), M. marinum produced a large amount of PGL-I, as previously reported. 21 When developing the lipid extract of this PGL-I-producing M. marinum strain, several spots in addition to PGL-I were...”
- “...Rha-2- O -methyltransferase; ML0128, rhamnosyl transferase; ML2346 and ML2347, Glc-3- and Glc-6- O -methyltransferases; and ML2348, glycosyl transferase. (b) Acetone-soluble lipids were extracted from crude lipids derived from mock and recombinant M. marinum and were fractionated into 16 fractions by HPTLC using C:M (95:5 v/v, three...”
- Mycobacterium lepromatosis as a Second Agent of Hansen's Disease
Deps, Frontiers in microbiology 2021 - “...2015 ). M. leprae genes encoding laminin-2 binding protein ML1683c and the six enzymes (ML0128, ML2348 ML0126, ML0127, ML23246c, and ML2347) required to produce the terminal trisaccharide moiety of phenolic glycolipid 1 (PGL-1) are highly conserved in M. lepromatosis ( Singh et al., 2015 ), suggesting...”
- A Macrophage Response to Mycobacterium leprae Phenolic Glycolipid Initiates Nerve Damage in Leprosy
Madigan, Cell 2017 - “...the integrating plasmid pWM122, which encodes the M.leprae genes ML0126, ML0127, ML0128, ML2346c, ML2347, and ML2348 under the M.fortuitum pBlaF promoter ( Tabouret etal., 2010 ). Kanamycin-resistant transformants were confirmed by PCR using primers targeting all six M.leprae genes ( Tabouret etal., 2010 ). A single...”
- Insight into the evolution and origin of leprosy bacilli from the genome sequence of Mycobacterium lepromatosis
Singh, Proceedings of the National Academy of Sciences of the United States of America 2015 - “...namely a rhamnosyl transferase (ML0128), a glucosyltransferase (ML2348), and four methyltransferases (ML0126, ML0127, ML23246c, and ML2347) (47). The genes...”
- Comparative phylogenomics of pathogenic and non-pathogenic mycobacterium
Prasanna, PloS one 2013 - “...an important regulator of genes in response of MTB to acid shock [34] . Rv1524, ML2348, MMAR_2353, MAV_3258, MUL_1529, MAB_4112c. Glycosyl transferase Reaeration of Rv under hypoxia (2.753), 3 mM ACE (3.577), MEN (6.26) Glycosyltransferases are the enzymes that synthesizeoligosaccharides,and lipopoly-sachharides [45] . Rv3484, ML2247, MMAR_4966,...”
- Mycobacterium leprae phenolglycolipid-1 expressed by engineered M. bovis BCG modulates early interaction with human phagocytes
Tabouret, PLoS pathogens 2010 - “...the two terminal residues and for the methylation of the first rhamnosyl residue: ML0128 and ML2348 encoding proteins with similarities to glycosyltransferases, and ML0126 , ML0127 , ML2346c and ML2347 encoding proteins with similarities to methyltransferases ( Figures 1B and 1C ) [18] , [19] ....”
- “...( Figure S1 ). In parallel, a second DNA fragment carrying ML2346c , ML2347 and ML2348 genes was inserted into the integrative vector pMV361 [25] to give pWM76 ( Figure S1 ). These two constructs were transferred independently or simultaneously into the BCG Rv2959c mutant. Lipids...”
- A glycosyltransferase involved in biosynthesis of triglycosylated glycopeptidolipids in Mycobacterium smegmatis: impact on surface properties
Deshayes, Journal of bacteriology 2005 - “...52% identity, respectively) and one in M. leprae (ML2348, with 56% identity). These homologs are characterized by a predicted glycosyltransferase domain of 150...”
- “...of M. tuberculosis (Rv1524 and Rv1526c) and M. leprae (ML2348) are located on branches distinct from those of M. smegmatis and M. avium (Fig. 6A), they are...”
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Afu7g04880 sterol glucosyltransferase, putative from Aspergillus fumigatus Af293
28% identity, 50% coverage
MSMEG_4740 Glycosyltransferase family protein 28 from Mycobacterium smegmatis str. MC2 155
29% identity, 98% coverage
- Orphan response regulator Rv3143 increases antibiotic sensitivity by regulating cell wall permeability in Mycobacterium smegmatis
Dong, Archives of biochemistry and biophysics 2020 (PubMed)- “...the sensitivity of the strain to rifampicin (RIF). MSMEG_4740 promoter binding with Rv3143, was screened out by surface plasmon resonance (SPR). Rv1524, the...”
- “...bromide (EB) accumulation, we found when Rv3143 or MSMEG_4740, or Rv1524 was overexpressed, the cell wall permeability of Mycobacterium smegmatis was increased....”
- MmpL8MAB controls Mycobacterium abscessus virulence and production of a previously unknown glycolipid family
Dubois, Proceedings of the National Academy of Sciences of the United States of America 2018 - “...most of the LOS locus from M. smegmatis (MSMEG_4731- MSMEG_4740) is not conserved in M. abscessus that does not produce LOS. MmpL8 of M. tuberculosis is...”
- Genetics of Capsular Polysaccharides and Cell Envelope (Glyco)lipids
Daffé, Microbiology spectrum 2014 - “...glycosyltransferase; MSMEG_4736 and MSMEG_4737, putative pyrruvylyl transferases; MSMEG_4738, hypothetical protein; Mtf, possible O -methyltransferase; Gtf (MSMEG_4740), putative glycosyltransferase; MmpL, putative inner membrane transporter. Figure 12 Structure of the predominant mannosyl--1-phosphomycoketide from Mtb H37Rv (See text for details) Figure 13 Structures of the phthiocerol dimycocerosates (PDIM), phenolic...”
- Identification of the polyketide synthase involved in the biosynthesis of the surface-exposed lipooligosaccharides in mycobacteria
Etienne, Journal of bacteriology 2009 - “...family GT1) MSMEG_4734 MSMEG_4735 gtf MSMEG_4738 MSMEG_4739 MSMEG_4740 mtf gtf MSMEG_4741 mmpL a b 1,017 Polyketide synthase-associated protein PF numbers...”
- “...putative glycosyltransferases share no significant homology, MSMEG_4740 (glycosyltransferase family protein 28) displays 50% sequence identity with each of...”
ABO_1783 glycosyl transferase, putative from Alcanivorax borkumensis SK2
28% identity, 91% coverage
- High-quality physiology of Alcanivorax borkumensis SK2 producing glycolipids enables efficient stirred-tank bioreactor cultivation
Karmainski, Frontiers in bioengineering and biotechnology 2023 - “...biosynthesis pathway of the glycine-glucolipid in A. borkumensis SK2 has not been elucidated. However, the ABO_1783 and ABO_2215 genes have been identified as potential contributors to glycolipid production ( Schneiker et al., 2006 ). These genes encode glycosyltransferases with similarities to rhamnosyltransferase B (RhlB) from P....”
- Marine derived biosurfactants: a vast potential future resource
Tripathi, Biotechnology letters 2018 - “...The genome sequence of A. borkumensis SK2 revealed its capacity for BS production. The glycosyltransferase (ABO_1783) similar to RhlB from P. aeruginosa and glycosyltransferase protein family 9 (ABO_2215) were found to be potentially involved in BS production. The A. borkumensis SK2 genome encodes other proteins involved...”
- Genome mining reveals the genus Xanthomonas to be a promising reservoir for new bioactive non-ribosomally synthesized peptides
Royer, BMC genomics 2013 - “...). Interestingly, in this strain, a XaPPTase-like gene (ABO_1782) is contiguous with the glycosyltransferase gene (ABO_1783) and the short NRPS gene (ABO_1784), confirming that a PPTase specific to PCP-domains is required for the unknown function of these unusually small NRPS systems. Similar short NRPS genes were...”
- Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis
Schneiker, Nature biotechnology 2006 - “...in A. borkumensis SK2, but genome annotation revealed candidate genes potentially involved in biosurfactant production. ABO_1783 and ABO_2215 encode glycosyltransferases, exhibiting significant homology with RhlB from Pseudomonas aeruginosa 24 and glycosyltransferase protein family 9, respectively. These gene products possibly provide the sugar moiety of the glucolipids,...”
Q6CUV2 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Kluyveromyces lactis (see paper)
27% identity, 33% coverage
SS1G_05901 hypothetical protein from Sclerotinia sclerotiorum 1980 UF-70
28% identity, 31% coverage
LEN_4536 glycosyltransferase from Lysobacter enzymogenes
28% identity, 86% coverage
O22678 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Avena sativa (see 2 papers)
CAB06081.1 UDP-Glc: sterol glucosyltransferase (Ugt80A1) (EC 2.4.1.173) (see protein)
ugt80A1 / CAB06081.1 UDP-glucose:sterol glucosyltransferase from Avena sativa (see paper)
26% identity, 64% coverage
AAC71702.1 GPL/6-deoxytalose α-L-1,2-rhamnosyltransferase A (RtfA;Orf2) (EC 2.4.1.-) (see protein)
AAD44209.1 rhamnosyltransferase A (RtfA) (EC 2.4.1.-) (see protein)
25% identity, 92% coverage
BAC22617.1 UDP-Glc: sterol 3-O-glucosyltransferase (PGGT-2) (EC 2.4.1.173) (see protein)
Q8H9B4 UDP-glucose:sterol 3-O-glucosyltransferase from Panax ginseng
28% identity, 66% coverage
- Microarray analysis of iron deficiency chlorosis in near-isogenic soybean lines.
O'Rourke, BMC genomics 2007 - “...Gm-c1028-3740 3.712 0.0359 GmTC217970 Q9LY38 Phagocytosis and Cell Motility Protein 3.00E-32 Gm-c1028-1088 4.181 0.1264 GmTC217285 Q8H9B4 UDP-glucosyltransferase 1.00E-179 Gm-c1028-963 7.149 0.0057 GmTC225698 Q2TE73 Zinc Ring Finger Protein 1.00E-108 This list of genes represents the genes identified as differentially expressed between the NILs under iron deficient conditions...”
MAB_4112c Putative glycosyltransferase GtfA from Mycobacterium abscessus ATCC 19977
26% identity, 90% coverage
- Global prevalence of Mycobacterium massiliense strains with recombinant rpoB genes (Rec-Mas) horizontally transferred from Mycobacterium abscessus: two major types, dominant circulating clone 7 and MLST ST46 sequence type
Kim, Microbiology spectrum 2024 - “...100.0% 100.0% 100.0% rmlB MAB_4111c NAD-dependent epimerase/dehydratase family protein CDS 100.0% 100.0% 100.0% 100.0% gtf3 MAB_4112c Glycosyltransferase CDS 100.0% 100.0% 100.0% 100.0% rmlA MAB_4113 rfbA CDS 100.0% 100.0% 100.0% 100.0% Rv1174 MAB_4114 Hemophore-related protein CDS 100.0% 100.0% 100.0% 100.0% mmpL4b MAB_4115c RND family transporter CDS 100.0%...”
- A dTDP-L-rhamnose 4-epimerase required for glycopeptidolipid biosynthesis in Mycobacterium abscessus
Aguilera-Correa, The Journal of biological chemistry 2024 - “...cross-links arabinogalactan to peptidoglycan. In addition, dTDP-L-Rha is also used by Gtf2 (MAB_4104) and Gtf3 (MAB_4112c) for GPL rhamnosylation ( blue arrows ). dTDP-6-d-Tal, produced from dTDP-L-Rha by the dTDP-Rha-4-epimerase (MAB_4111c/Tle) serves as the substrate for the talosyltransferase Gtf1 ( green arrows ), which adds one...”
- Mabellini: a genome-wide database for understanding the structural proteome and evaluating prospective antimicrobial targets of the emerging pathogen Mycobacterium abscessus
Skwark, Database : the journal of biological databases and curation 2019 - “...230 229 99.6 28.3 0.888 0.643 3.567 MAB_4111c 353 348 98.6 31.2 0.926 0.923 3.345 MAB_4112c 440 418 95 19.3 0.96 0.684 3.44 MAB_4113 288 287 99.7 60.1 0.887 0.833 2.651 MAB_4115c 987 331 33.5 16.3 0.82 0.405 3.421 MAB_4116c 959 939 97.9 13.5 0.964 0.567...”
- “...proteins): MbtH-like protein (MAB_4100c), Fmt (MAB_4103c), Rmt3 (MAB_4105c), Rmt4 (MAB_4108c), Rmt2 (MAB_4109c), RmlB (MAB_4111c), Gtf3 (MAB_4112c), RmlA (MAB_4113), MmpL4b (MAB_4115c), MmpL4a (MAB_4116c) and MMpS4 (MAB_4117c) with coverages for the best models in each case ranging from 33.5% to 99.7%. Web-page front end The website is accessible...”
- Subinhibitory Doses of Aminoglycoside Antibiotics Induce Changes in the Phenotype of Mycobacterium abscessus
Tsai, Antimicrobial agents and chemotherapy 2015 - “...MAB_4103c MAB_4104 MAB_4105c MAB_4110c MAB_4111c MAB_4112c MAB_4113 MAB_4114 MAB_4115c MAB_4116c MAB_4117c MAB_4454c MAB_4459c MAB_4633 gap-like fadD23...”
- Rough colony morphology of Mycobacterium massiliense Type II genotype is due to the deletion of glycopeptidolipid locus within its genome
Kim, BMC genomics 2013 - “...MSMEG0376 48 rmlA MAB_4113 Glucose-1-phosphate thymidylyltransferase MMAS_40560 100 MASS_4112 100 MC1622c 97 MSMEG0377 85 gtf3 MAB_4112c Putative glycosyltransferase GtfA MMAS_40540 98 MASS_4110 99 MC1623 88 MSMEG0378 70 rmlB MAB_4111c Putative epimerase/dehydratase MMAS_40530 99 MASS_4109 99 MC1624 93 MSMEG0379 78 atf2 MAB_4110c Probable acetyltransferase AtfA MMAS_40520 97...”
- Comparative phylogenomics of pathogenic and non-pathogenic mycobacterium
Prasanna, PloS one 2013 - “...genes in response of MTB to acid shock [34] . Rv1524, ML2348, MMAR_2353, MAV_3258, MUL_1529, MAB_4112c. Glycosyl transferase Reaeration of Rv under hypoxia (2.753), 3 mM ACE (3.577), MEN (6.26) Glycosyltransferases are the enzymes that synthesizeoligosaccharides,and lipopoly-sachharides [45] . Rv3484, ML2247, MMAR_4966, MAV_0673, MUL_4040, MAB_0560. Hypothetical...”
K4C3H8 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Solanum lycopersicum (see paper)
27% identity, 64% coverage
TERG_00990 sterol 3-beta-glucosyltransferase from Trichophyton rubrum CBS 118892
28% identity, 60% coverage
- Pharmacological Potential and Chemical Characterization of Bridelia ferruginea Benth.-A Native Tropical African Medicinal Plant
Mahomoodally, Antibiotics (Basel, Switzerland) 2021 - “...epigallocatechin, quercetin, and gallic acid with multiple dermatophytes enzymes, including ATP synthase (TERG_07188), UDP-glucose:sterol glucosyltransferase (TERG_00990) and xanthine dehydrogenases (TERG_06183, TERG_04145, TERG02032) ( Figure 2 and Figure 3 ), involved in the energetic metabolism. Overall, the reported bioinformatics prediction is consistent with the antifungal effects induced...”
AAN28688.1 GPL/3,4-di-O-Me-rhamnose α-L-1,2-(3-O-methyl-)rhamnosyltransferase (Gtf3;MSMEG_0385) (EC 2.4.1.-) (see protein)
28% identity, 94% coverage
Mb1553c Probable glycosyltransferase from Mycobacterium bovis AF2122/97
Rv1526c Probable glycosyltransferase from Mycobacterium tuberculosis H37Rv
29% identity, 93% coverage
- Whole genome sequencing of the monomorphic pathogen Mycobacterium bovis reveals local differentiation of cattle clinical isolates
Lasserre, BMC genomics 2018 - “...5 : Table S3). Among them we found pks12 and two glycosyltransferases ( Mb1551 and Mb1553c ) with identity scores between 86% and 93% in nine of the strains. Manual inspection of the alignment shows that several unequivocal mapped reads support these polymorphisms, evidencing that the...”
- “...: Table S6. The genes with low identity values mentioned before, pks12 , Mb1551 and Mb1553c , had the highest detected SNP density . Fig. 4 Visualization of the density of variants in the entire length of the reference genome (AF2122/97). Inner rays represent the variant...”
- Comparative Study of the Molecular Basis of Pathogenicity of M. bovis Strains in a Mouse Model
Cheng, International journal of molecular sciences 2018 - “...Anaerobic respiration Nitrate reductase - narG Mb1267 Cell surface components Trehalose-recycling ABC transporter - lpqY Mb1553C Cell surface components GPL locus - gtf1 Mb1766C Anaerobic respiration Nitrate/nitrite transporter narK2 - Mb2139C Proteases Proteasome-associated proteins mpa - Mb2404C Metal uptake Mycobactin - mbtB Mb2439C Secreted proteins Enhanced...”
- Diversification of gene content in the Mycobacterium tuberculosis complex is determined by phylogenetic and ecological signatures
Silva-Pereira, Microbiology spectrum 2024 - “...gtf3 gene, a member of the GPL locus, in addition to wbbL2 (Rv1525) and gtf (Rv1526c), is part of the RD147C of M. tuberculosis L1 strains, and gtf (Rv1526c) is also pseudogenized in M. caprae strains. The narx gene is pseudogenized in dassie bacillus ( Fig....”
- The rate and role of pseudogenes of the Mycobacterium tuberculosis complex
Soler-Camargo, Microbial genomics 2022 - “...is intact in M. canettii genomes. The putative glycosyltransferases 3 ( gtf3 ) Rv1524 and Rv1526c of the GPL locus are pseudogenized in 16 M . africanum L6 strains and in five M. bovis strains, respectively. The pks2 (Rv3825c), a gene of the sulfolipid biosynthesis and...”
- Connection between two historical tuberculosis outbreak sites in Japan, Honshu, by a new ancestral Mycobacterium tuberculosis L2 sublineage
Guyeux, Epidemiology and infection 2022 - “...genes: as an example, DRR034363 had Rv1081 to 1084c deleted, DRR034416 was missing Rv1523 to Rv1526c. (Supplementary Table S7). These deletions confirm that phylogenetically linked MTB genomes sometimes harbour strain-specific dependent deletions due to recombination events. In silico VNTR copy number computation using CAST and comparison...”
- Heat-killed Mycolicibacterium aurum Aogashima: An environmental nonpathogenic actinobacteria under development as a safe novel food ingredient
Nouioui, Food science & nutrition 2021 - “...<95% 0 fad E5 orf01415; orf05079 Rv0244c 82.7 and 66.1 <95% 0 gtf 1 orf01592 Rv1526c 48.8 <95% 1.3 gtf 2 orf01265; orf01577 Rv1524 58.2 and 53.8 <95% 9E162 mmp L10 orf01421 Rv1183 57.2 <95% 0 mmp S4 orf00497; orf04089 Rv0451c 47.5 <95% 3E47 mp s1...”
- Genome-wide identification of the context-dependent sRNA expression in Mycobacterium tuberculosis
Ami, BMC genomics 2020 - “...<2.2e-16) (Fig. 6 ). Genes Rv3003c, Rv1924c, Rv3150, Rv1937, Rv1728c, Rv1626, Rv1308, Rv0544c, Rv0532 and Rv1526c are the predicted targets for this sRNA (Additional file 11 : Table S5b). Subsequent gene expression analysis revealed that the predicted targets Rv1308, Rv3003c, Rv1924c, Rv1728c, Rv1626 and Rv3150 showed...”
- Whole genome sequencing, analyses of drug resistance-conferring mutations, and correlation with transmission of Mycobacterium tuberculosis carrying katG-S315T in Hanoi, Vietnam
Hang, Scientific reports 2019 - “...Rv1050, Rv1069c, Rv1085c, Rv1178, Rv1225c, Rv1273c, Rv1313c, Rv1318c, Rv1372, Rv1395, Rv1425, Rv1455, Rv1488, Rv1498A, Rv1499, Rv1526c, Rv1610, Rv1667c, Rv1683, Rv1702c, Rv1707, Rv1714, Rv1725c, Rv1726, Rv1728c, Rv1729c, Rv1739c, Rv1749c, Rv1815, Rv1825, Rv1828, Rv1831, Rv1835c, Rv1879, Rv1895, Rv1941, Rv1999c, Rv2017, Rv2023A, Rv2024c, Rv2025c, Rv2038c, Rv2074, Rv2134c, Rv2143, Rv2164c,...”
- Genetic signatures of Mycobacterium tuberculosis Nonthaburi genotype revealed by whole genome analysis of isolates from tuberculous meningitis patients in Thailand
Coker, PeerJ 2016 (no snippet) - Analysis of IS6110 insertion sites provide a glimpse into genome evolution of Mycobacterium tuberculosis
Roychowdhury, Scientific reports 2015 - “...<20%) Annotation 888800888900 100 2.46 Intergenic (Rv0794c-Rv0795) 932100932200 88.88 0 Intergenic (Rv0835-Rv0836c) 17213001721400 100 0 Rv1526c (glycosyltransferase) 19986001998700 88.88 0 Intergenic (Rv1765c-Rv1765A) 20388002038900 88.88 0 Intergenic (Rv1798-Rv1799) EAI6 (BGD1) Percentage of EAI6 isolates (threshold >75%) Percentage of other L1 (threshold <20%) Annotation 888700888800 97.87 3.92 Intergenic...”
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XAC3921 glucosyltransferase from Xanthomonas axonopodis pv. citri str. 306
30% identity, 89% coverage
- The Methyltransferase HemK Regulates the Virulence and Nutrient Utilization of the Phytopathogenic Bacterium Xanthomonas citri Subsp. citri
Shi, International journal of molecular sciences 2022 - “...endoproteinase Arg-C, degrading host defense proteins XAC0612 engXCA 1.3 cellulase XAC3120 glk 1.3 glucose kinase XAC3921 ugt 1.2 glucosyltransferase XAC1556 fucP 1.3 glucose-galactose transporter XAC1557 scrK 1.2 fructokinase XAC3489 fyuA 2.3 TonB-dependent receptor XAC3490 XAC3490 1.9 amylosucrase or alpha amylase XAC0465 XAC0465 1.0 metalloproteinase XAC4327 uahA...”
- Diffusible signal factor (DSF)-mediated quorum sensing modulates expression of diverse traits in Xanthomonas citri and responses of citrus plants to promote disease
Li, BMC genomics 2019 - “...egl /XAC0029 and engXCA/ XAC0612) , glycosyl transferase ( gtrB/ XAC1038/XAC2125 , XAC3533, and ugt/ XAC3921 ), glycosyl hydrolase (XAC3073), glycogen synthase ( glgA /XAC0425 and glgY /XAC0429), glucose dehydrogenase ( gcd/ XAC1633/XAC3212 ), glucokinase ( glk /XAC3120), and transporter ( araJ/ XAC1363 and yieO/ XAC2494...”
- “...hydrolase XAC3120 glk 1.36 glucokinase XAC3212 gcd 1.05 glucose dehydrogenase XAC3533 1.23 Glycosyltransferase, GT2 family XAC3921 ugt 1.52 glucosyltransferase XAC0217 lgtB 1.06 glycosyltransferase XAC0299 2.16 polysaccharide /chitin deacetylase XAC0575 ganB 1.98 arabinogalactan endo-1,4-beta-galactosidase XAC1286 abfA 1.09 alpha-L-arabinofuranosidase XAC1308 bga 1.18 beta-galactosidase XAC1309 galA 1.49 arabinogalactan endo-1,4-beta-galactosidase...”
MSMEG_0385 hypothetical glycosyl transferase from Mycobacterium smegmatis str. MC2 155
28% identity, 94% coverage
A0A2D1N4Z8 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Solanum lycopersicum (see paper)
26% identity, 67% coverage
LOC115712970 sterol 3-beta-glucosyltransferase UGT80A2 from Cannabis sativa
26% identity, 65% coverage
K4BS77 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Solanum lycopersicum (see paper)
26% identity, 64% coverage
MMAR_2353 UDP-glycosyltransferase from Mycobacterium marinum M
28% identity, 97% coverage
- Mycobacterium marinum mmar_2318 and mmar_2319 are Responsible for Lipooligosaccharide Biosynthesis and Virulence Toward Dictyostelium
Chen, Frontiers in microbiology 2015 - “...20 loci, six genes ( losA , mmar_2318 , mmar_2319 , wecE , mmar_2323 and mmar_2353 ) were located in the lipooligosaccharide (LOS) synthesis cluster. LOS are antigenic glycolipids and the core LOS structure from LOS-I to LOS-IV have been reported to exist in M. marinum...”
- “...2-C10, 2-G4 mmar_2320 wecE , pyridoxal phosphate-dependent enzyme 10-A11 mmar_2323 Conserved hypothetical transmembrane protein 13-B8 mmar_2353 UDP-glycosyltransferase Rv1524 12-E11 Upstream of mmar_2684 PPE family protein PPE32 18-G4 mmar_3183 Hypothetical alanine rich protein 18-D7 Upstream of mmar_3375 Conserved hypothetical protein 17-A5, 18-H5, 12-A1 mmar_4263 Conserved hypothetical protein...”
- Increased phagocytosis of Mycobacterium marinum mutants defective in lipooligosaccharide production: a structure-activity relationship study
Alibaud, The Journal of biological chemistry 2014 - “...losA mutants and single mutants in MMAR_2315, MMAR_2319, MMAR_2353, and MMAR_5103. In addition, we also included in this study an MMAR_2343/papA4 mutant...”
- “...by the accumulation of LOS-III (Fig. 1B). The MMAR_2353 mutant was characterized by a strongly reduced LOS profile as reported earlier (14) (data not...”
- Comparative phylogenomics of pathogenic and non-pathogenic mycobacterium
Prasanna, PloS one 2013 - “...important regulator of genes in response of MTB to acid shock [34] . Rv1524, ML2348, MMAR_2353, MAV_3258, MUL_1529, MAB_4112c. Glycosyl transferase Reaeration of Rv under hypoxia (2.753), 3 mM ACE (3.577), MEN (6.26) Glycosyltransferases are the enzymes that synthesizeoligosaccharides,and lipopoly-sachharides [45] . Rv3484, ML2247, MMAR_4966, MAV_0673,...”
- Unexpected link between lipooligosaccharide biosynthesis and surface protein release in Mycobacterium marinum
van, The Journal of biological chemistry 2012 - “...Fatty acyl AMP ligase UDP-glycosyltransferase mmar_2353 mmar_2355 papA3 Conserved polyketide synthase-associated protein mmar_2405 mmar_4419 mmar_5170 cphB...”
- “...(mmar_2327) B7 (galE6) A2 (pks5) A4 (fadD25) A1/A7 (mmar_2353) A5 (papA3) A6 (cphB) A9 (whiB4) No LOS-II, -III, -IV; accumulation LOS-I No LOS-IV; accumulation...”
FVEG_00073 hypothetical protein from Fusarium verticillioides 7600
27% identity, 45% coverage
A5DNB9 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Meyerozyma guilliermondii (see paper)
28% identity, 25% coverage
MAV_3258 Glycosyltransferase family protein 28 from Mycobacterium avium 104
28% identity, 94% coverage
- Comparative phylogenomics of pathogenic and non-pathogenic mycobacterium
Prasanna, PloS one 2013 - “...regulator of genes in response of MTB to acid shock [34] . Rv1524, ML2348, MMAR_2353, MAV_3258, MUL_1529, MAB_4112c. Glycosyl transferase Reaeration of Rv under hypoxia (2.753), 3 mM ACE (3.577), MEN (6.26) Glycosyltransferases are the enzymes that synthesizeoligosaccharides,and lipopoly-sachharides [45] . Rv3484, ML2247, MMAR_4966, MAV_0673, MUL_4040,...”
- Discovery of stable and variable differences in the Mycobacterium avium subsp. paratuberculosis type I, II, and III genomes by pan-genome microarray analysis
Castellanos, Applied and environmental microbiology 2009 - “...or MAV_2223 [IS6120]) and six LSPs (INDEL3, or MAV_3258 to MAV_3270 [previously described as MAV17 {42}]; INDEL5, or MAV_2978 to MAV_2998 [previously described...”
- “...MAV_0775 (MAP0660 to MAP0661) MAP0758 to MAP0774c MAP0852 to MAP0866 MAP1231 to MAP1236c MAV_3258 to MAV_3270 . . . . . . . . . . . . . . . . . V . . . ....”
SS1G_09252 hypothetical protein from Sclerotinia sclerotiorum 1980 UF-70
26% identity, 52% coverage
RHTO_07138 sterol 3-beta-glucosyltransferase, glycosyltransferase family 1 protein from Rhodotorula toruloides NP11
26% identity, 24% coverage
- Regulation of autophagy and lipid accumulation under phosphate limitation in Rhodotorula toruloides
Wang, Frontiers in microbiology 2022 - “...NaN Atg20 RHTO_04181 Sorting nexin-41 0.67 NaN Atg24 RHTO_04976 Lipid binding protein 1.72 NaN Atg26 RHTO_07138 Sterol 3-beta-glucosyltransferase, glycosyltransferase family 1 protein 1.38 NaN Atg27 RHTO_06153 Autophagy-related protein 27 2.00 NaN NaN, not detectable. Autophagy and lipid accumulation influenced by Atg9 To investigate whether Atg9 is...”
- Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production
Tiukova, Biotechnology for biofuels 2019 - “...Additionally, at this stage in xylose-grown cells, the levels of autophagy-related protein Rhto_06526, Rhto_02366 and Rhto_07138 were upregulated. Interestingly, several proteins involved in autophagy (Rhto_06526, Rhto_01636, Rhto_05541) were upregulated in cells cultivated in xylose as compared to glucose at early exponential phase. This might indicate stress...”
SPSK_05928 glucosylltransferase family 28 protein from Sporothrix schenckii 1099-18
39% identity, 17% coverage
- The Search for Cryptic L-Rhamnosyltransferases on the Sporothrix schenckii Genome
Mora-Montes, Journal of fungi (Basel, Switzerland) 2022 - “...5-TCGACCTTCTTGATGTTGG-3 for the endogenous control; 5- AACAGATTGAGGCTCTGGGC-3 and 5-AGTAAGTCTGGGTTCGCCAC-3 for SPSK_05538; 5-CAGAACGGAAAGACGGGACA-3 and 5-ACCACCGTTGTTGTTGACCT-3 for SPSK_05928; 5-AGGAATGACCATCAGCGCAA-3 and 5-AAACGCTCGGGAAAGTCCAT-3 for SPSK_01368; 5-AGCCACGAACGTCTCTTCAG-3 and 5-CTTCAAACGTGCCTGATGGC-3 for SPSK_04821; and 5-GTTTGTCCCCGACGTTTTGG-3 and 5-GCCGCGAGATGAAAAAGACG-3 for SPSK_01110. 2.7. Heterologous Gene Expression in Escherichia coli and Protein Purification The open reading frames...”
- “...the S. schenckii proteome using this HMM profile, recovering five protein sequences (SPSK_01368, SPSK_04821, SPSK_05538, SPSK_05928, and SPSK_01110) ranging in sizes from 232 aa to 1836 aa ( Table 1 ). Four of the five sequences were automatically annotated as glycosyltransferases, and a fifth sequence was...”
ATG26_CANAL / Q5A950 Sterol 3-beta-glucosyltransferase; Autophagy-related protein 26; UDP-glycosyltransferase 51; EC 2.4.1.-; EC 2.4.1.173 from Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) (see paper)
Q5A950 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Candida albicans (see paper)
27% identity, 24% coverage
- function: Sterol glycosyltransferase responsible for the glycosylation of ergosterol to form ergosterol-glucoside.
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
catalytic activity: ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D- glucoside + UDP + H(+) (RHEA:61836) - Effect of the Ethyl Acetate Fraction of Eugenia uniflora on Proteins Global Expression during Morphogenesis in Candida albicans
Silva-Rocha, Frontiers in microbiology 2017 - “...protein 2 ACB2 Protein transport P61868 10,084 4.74 Decrease 31 Sterol 3-beta-glucosyltransferase ATG26 Membrane synthesis Q5A950 170,545 5.57 32 ATP-dependent RNA helicase DBP10 DBP10 Ribossomal structure Q5ANB2 103,561 9.64 34 U6 snRNA-associated Sm-like protein LSm3 LSM3 RNA processing P57743 10,030 4.29 35 Actin cytoskeleton-regulatory complex protein...”
Q9WW66 Glycosyltransferase gtfB from Mycobacterium avium
28% identity, 94% coverage
UGT51C1 sterol 3-beta-glucosyltransferase; EC 2.4.1.173 from Candida albicans (see paper)
27% identity, 24% coverage
- CharProtDB CGD description: UDP-glucose
AAD29571.1 UDP-Glc: sterol glucosyltransferase (UGT51C1) (EC 2.4.1.173) (see protein)
27% identity, 24% coverage
ATG26_KOMPG / Q9Y751 Sterol 3-beta-glucosyltransferase; Autophagy-related protein 26; Peroxisome degradation protein 3; Pexophagy zeocin-resistant mutant protein 4; UDP-glycosyltransferase 51; EC 2.4.1.-; EC 2.4.1.173 from Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris) (see 5 papers)
Q9Y751 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Komagataella pastoris (see 3 papers)
CAY71393.1 UDP-Glc: sterol glucosyltransferase (UGT51B1;PAS_chr4_0167) (EC 2.4.1.173) (see protein)
26% identity, 32% coverage
- function: Sterol glycosyltransferase responsible for the glycosylation of ergosterol to form ergosterol-glucoside (PubMed:10224056). Shows also activity in vitro on other sterols such as cholesterol, beta- sitosterol, stigmasterol and tomatidine (PubMed:10224056). Probable sterol 3-beta-glucosyltransferase that mediates autophagic degradation of peroxisomes (pexophagy) (PubMed:11856375, PubMed:12839986, PubMed:14585290, PubMed:19605559).
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
catalytic activity: ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D- glucoside + UDP + H(+) (RHEA:61836)
5gl5A / Q06321 Sterol 3-beta-glucosyltransferase (ugt51) from saccharomyces cerevisiae (strain atcc 204508 / s288c): udpg complex (see paper)
27% identity, 80% coverage
- Ligand: uridine-5'-diphosphate-glucose (5gl5A)
ASPSYDRAFT_91437 uncharacterized protein from Aspergillus sydowii CBS 593.65
34% identity, 19% coverage
- Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1
Bu, BMC genomics 2020 - “...dihydroflavonol-4-reductase ASY000353 denovo DFR_4 dihydroflavonol-4-reductase ASY001313 denovo DFR_5 dihydroflavonol-4-reductase ASPSYDRAFT_162316 Reference-guided LDOX leucoanthocyanidin dioxygenase/anthocyanidin synthase ASPSYDRAFT_91437 Reference-guided UGTs_1 glycosyltransferase ASPSYDRAFT_33013 Reference-guided UGTs_2 glycosyltransferase ASPSYDRAFT_86678 Reference-guided UGTs_3 glycosyltransferase ASY002269 denovo UGTs_4 glycosyltransferase ASY000865 denovo OMT O-methytransferase Fig. 3 Statistics of gene functions related to anthocyanin metabolism in...”
- “...Finally, three lncRNAs appeared to regulate two genes involved in anthocyanin synthesis. One functional gene, ASPSYDRAFT_91437, and three lncRNA genes, MSTRG.7153.1, MSTRG.3144.1, and MSTRG.3145.1, were differentially expressed in G2 and G8 (Fig. 5 b, Table 3 ). The correlation between the function and lncRNA genes involved...”
MAB_4107c Glycosyltransferase GtfA from Mycobacterium abscessus ATCC 19977
25% identity, 94% coverage
U5D0T8 Uncharacterized protein from Amborella trichopoda
27% identity, 71% coverage
- Exploring the Phytochemical Landscape of the Early-Diverging Flowering Plant Amborella trichopoda Baill
Wu, Molecules (Basel, Switzerland) 2019 - “...(486), U5CWM7 (431), U5CX27 (363), U5CXJ9 (482), U5CXN6 (489), U5CYW0 (453), U5D008 (374), U5D0B7 (562), U5D0T8 (551), U5D1 9 (469), U5D276 (470), U5D2S8 (473), U5D3G1 (450), U5D3V5 (434), U5D3V6 (463), U5D531 (408), U5D5 3 (411), U5D5 9 (438), U5D5Z2 (488), U5D602 (452), U5D7I9 (490), U5D817 (481),...”
XALc_1144 putative glycosyltransferase protein from Xanthomonas albilineans
31% identity, 88% coverage
- Genome mining reveals the genus Xanthomonas to be a promising reservoir for new bioactive non-ribosomally synthesized peptides
Royer, BMC genomics 2013 - “...in other NRPS loci of strain GPE PC73. Their respective overlapping glycosyltransferase genes (XALc_0365 and XALc_1144) share 62% amino acid similarity (Additional file 3 ). The C-domains of these two short NRPS genes segregate in the same phylogenetic clade as starter C-domains identified by Rausch et...”
- “...XaS3 shares 92% and 62% amino acid similarity with the two glycosyltransferase genes XALc_0365 and XALc_1144, respectively, of strain GPE PC73 (Additional file 3 ). Genome mining uncovers a META-B-like NRPS gene cluster in strains X11-5A, BAI3 and XaS3 Genes similar to those from the gene...”
ATG26_YEAST / Q06321 Sterol 3-beta-glucosyltransferase; Autophagy-related protein 26; UDP-glycosyltransferase 51; EC 2.4.1.-; EC 2.4.1.173 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
Q06321 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Saccharomyces cerevisiae (see 5 papers)
AAB67475.1 UDP-Glc: sterol glucosyltransferase (Atg26;YLR189c;UGT51) (EC 2.4.1.173) (see protein)
NP_013290 sterol 3-beta-glucosyltransferase from Saccharomyces cerevisiae S288C
YLR189C Atg26p from Saccharomyces cerevisiae
26% identity, 29% coverage
- function: Sterol glycosyltransferase responsible for the glycosylation of ergosterol to form ergosterol-glucoside (PubMed:10224056, PubMed:30395931). Also shows activity in vitro on other sterols such as cholesterol, beta-sitosterol, stigmasterol and tomatidine (PubMed:10224056). In contrasts to what is observed in Pichia pastoris and Aspergillus oryzae, is not involved in cytoplasm to vacuole transport (Cvt), pexophagy or nonselective autophagy in Saccharomyces cerevisiae (PubMed:17012830).
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
catalytic activity: ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D- glucoside + UDP + H(+) (RHEA:61836) - Insight into vital role of autophagy in sustaining biological control potential of fungal pathogens against pest insects and nematodes
Ying, Virulence 2019 - “...OAA71568 OAA64158 EFY91127 KJK79963 EFY99206 OAA40571 OAA63756 EGX43379 KJZ78598 25 ESW97416 26 ESW96191 ANZ76118 CAY71393 Q06321 KZZ90874 KZZ92672 EJP71037 OAA45995 ATY62412 OAA73722 OAA81945 EFY86996 KJK79229 EFY97936 OAA36440 OAA59786 EGX51249 KJZ73456 27 ESW97460 ANZ75892 CAY69817 P46989 KZZ98281 KZZ88672 EJP63614 OAA44065 EGX88907 OAA52851 OAA76046 EFY88154 KJK81470 EFZ02138 OAA41305...”
- Enfumafungin synthase represents a novel lineage of fungal triterpene cyclases
Kuhnert, Environmental microbiology 2018 - “...GTs are nested between a cluster containing a functionally characterized sterol GT of Saccharomyces cerevisiae (Q06321) ( Warnecke et al ., 1999 ) and various clades with GTs that show homology to the latter. These data imply that the enfumafungin GT is derived from a sterol...”
- Ordered Coimmobilization of Multimeric Enzyme Arrays with Enhanced Biocatalytic Cascade Performance.
Ali, ACS applied bio materials 2021 (PubMed)- GeneRIF: Ordered Coimmobilization of Multimeric Enzyme Arrays with Enhanced Biocatalytic Cascade Performance.
- Structural dissection of sterol glycosyltransferase UGT51 from Saccharomyces cerevisiae for substrate specificity.
Chen, Journal of structural biology 2018 (PubMed)- GeneRIF: The structural analysis indicate that a long hydrophobic cavity, 9.2A in width and 17.6A in length located at the N-terminal domain of sterol glycosyltransferase UGT51, is suitable for the accommodation of sterol acceptor substrates.
- Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis
Stucky, Journal of experimental botany 2015 - “...confirmed by sequencing the isolated plasmids. Yeast UGT51 deletion Yeast ( S. cerevisiae ) strain YLR189C (Thermo Scientific USA) contains a KanMX cassette replacing the native UGT51 ( UGT51A1 ) ORF. Genomic DNA was isolated from YLR189C and the KanMX cassette was amplified with primers flanking...”
- High-throughput biochemical fingerprinting of Saccharomyces cerevisiae by Fourier transform infrared spectroscopy
Kohler, PloS one 2015 - “..., 2 YLR133W CKI1 26 YDR018C YDR018C 67 WT WT BY4743 27 YDR072C IPT1 68 YLR189C ATG26 28 YDR147W EKI1 69 1 YLR228C ECM22 29 YDR213W UPC2 70 YML059C NTE1 30 2 WT WT BY4743 71 YML008C ERG6 31 1 , 2 YDR294C DPL1 72 1...”
- Autophagy contributes to regulation of nuclear dynamics during vegetative growth and hyphal fusion in Fusarium oxysporum
Corral-Ramos, Autophagy 2015 - “...YFR021W YOL082W YDL113C YPL100W YCL038C YLR431C YJL036W YLR189C YJL178C XP_001821228 b YPL166W CCA39286 YDR022C YIL146C YLR356W YOL083W C4QVX6 YJL185C C4R8D7...”
- Widespread reorganization of metabolic enzymes into reversible assemblies upon nutrient starvation
Narayanaswamy, Proceedings of the National Academy of Sciences of the United States of America 2009 - “...YNL209W YOR027W YIL078W YDR074W YGR019W YKL035W YLR189C YJL130C YGR094W Purine nucleotide biosynthesis Purine nucleotide biosynthesis Purine nucleotide...”
- Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum
Warnecke, The Journal of biological chemistry 1999 (PubMed)- “...These genes from Saccharomyces cerevisiae (UGT51 5 YLR189C), Pichia pastoris (UGT51B1), Candida albicans (UGT51C1), and Dictyostelium discoideum (ugt52) were...”
- “...P. pastoris, and D. discoideum TM S. cerevisiae--UGT51 (YLR189C, GenBank accession number U17246) is a gene located on chromosome XII of S. cerevisiae. We...”
AAD28546.1 UDP-Glc: sterol glucosyltransferase (Ugt52) (EC 2.4.1.173) (see protein)
24% identity, 39% coverage
Q6BN88 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Debaryomyces hansenii (see paper)
28% identity, 22% coverage
ATG26_PICAN / A7KAK6 Sterol 3-beta-glucosyltransferase; Autophagy-related protein 26; EC 2.4.1.-; EC 2.4.1.173 from Pichia angusta (Yeast) (Hansenula polymorpha) (see paper)
A7KAK6 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Ogataea angusta (see paper)
27% identity, 32% coverage
- function: Sterol glycosyltransferase responsible for the glycosylation of ergosterol to form ergosterol-glucoside (By similarity). Mediates autophagic degradation of peroxisomes (pexophagy) (PubMed:17204848).
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
catalytic activity: ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D- glucoside + UDP + H(+) (RHEA:61836)
AAN77910.1 UDP-Glc: sterol glucosyltransferase (UGT51D1) (EC 2.4.1.173) (see protein)
28% identity, 58% coverage
XC_3951 glucosyltransferase from Xanthomonas campestris pv. campestris str. 8004
28% identity, 89% coverage
- RpfC regulates the expression of the key regulator hrpX of the hrp/T3SS system in Xanthomonas campestris pv. campestris
Jiang, BMC microbiology 2018 - “...Kan r ; Tc r This work XB005 8004/pL6 hrpXsacB with a Tn5 insertion in XC_3951 ; Rif r ; Kan r ; Tc r This work XB006 8004/pL6 hrpXsacB with a Tn5 insertion in XC_0124 ; Rif r ; Kan r ; Tc r This...”
- “...details), revealing that the mutations lie in the ORFs XC_4007 (XB001), XC_2333 (XB003), XC_1192 (XB004), XC_3951 (XB005) and XC_0124 (XB006), and the intergenetic region between the ORFs XC_1510 and XC_1511 (XB002), respectively. Interestingly, the ORF XC_2333 is the rpfC gene. The others were annotated to encode...”
MAV_3994 glycosyltransferase GtfB from Mycobacterium avium 104
36% identity, 41% coverage
XP_003044011 uncharacterized protein from Fusarium vanettenii 77-13-4
37% identity, 13% coverage
- Alternative Functional rad21 Paralogs in Fusarium oxysporum
Pareek, Frontiers in microbiology 2019 - “...related species to F. oxysporum and in other hypocreales but is found in Fusarium solani (XP_003044011), Stachybotrys chartarum (S7711_01263), Stachybotrys chloronata , and Fusarium nygamai (FNYG_15271) for this reason it was termed rad21 non-conserved ( rad21 nc) ( Figure 1A ). To reveal the origin of...”
MSMEG_0389 hypothetical glycosyl transferase from Mycobacterium smegmatis str. MC2 155
24% identity, 88% coverage
- Compartmentalization of galactan biosynthesis in mycobacteria
Savková, The Journal of biological chemistry 2024 - “...as mannosyl transferase PimB (MSMEG_4253), phosphatidylserine decarboxylase Psd (MSMEG_0861), polyprenol-monophosphomannose synthase Ppm1 (MSMEG_3859), glycosyltransferase Gtf1 (MSMEG_0389), geranylgeranyl reductase (MSMEG_2308), dihydroorotate dehydrogenase PyrD (MSMEG_4198), and a putative membrane protein (MSMEG_1944) ( 18 ). For five of these proteins (GlfT2, Gtf1, MSMEG_2308, PyrD, and MSMEG_1944), their localization to...”
- Spatiotemporal localization of proteins in mycobacteria
Zhu, Cell reports 2021 - “...in Figure 1F . Using the cell pole-associated protein Wag31 and the subpolar protein Gtf1 (MSMEG_0389) surrogates ( Figure 1G ), we found that our bimodal interpolation method is advantageous at preserving the polar signal topology ( Figures S4C - S4H ), which enables cell-to-cell comparisons...”
- Mycobacterium smegmatis PafBC is involved in regulation of DNA damage response
Fudrini, Scientific reports 2017 - “...MSMEG_1773 Uncharacterized protein 1.7 MSMEG_0224 Omethyltransferase 1.6 MSMEG_3935 Uncharacterized protein 1.7 MSMEG_1802 ChaB protein 2.3 MSMEG_0389 Gtf1 1.5 MSMEG_3940 UspA 1.5 MSMEG_1950 Uncharacterized protein 1.9 MSMEG_0408 Polyketide synthase 1.5 MSMEG_3945 Univ. stress protein 1.8 MSMEG_1951 Uncharacterized protein 1.9 MSMEG_0633 PAP2 family protein 1.7 MSMEG_3959 GntR family...”
- Spatially distinct and metabolically active membrane domain in mycobacteria
Hayashi, Proceedings of the National Academy of Sciences of the United States of America 2016 - “...GlfT2 (MSMEG_6403, UDPgalactosyl transferase), Gtf1 (MSMEG_0389, glycosyltransferase), a geranylgeranyl reductase (MSMEG_2308), PyrD (MSMEG_4198, dihydroorotate...”
- Identification of the polyketide synthase involved in the biosynthesis of the surface-exposed lipooligosaccharides in mycobacteria
Etienne, Journal of bacteriology 2009 - “...identified in the GPL biosynthesis locus, MSMEG_0385, MSMEG_0389, and MSMEG_0392. Taken together, these observations strongly suggested that the cluster of...”
AAC71701.1 GPL/6-deoxytalose α-L-1,2-rhamnosyltransferase A (GtfA) (EC 2.4.1.-) (see protein)
O68999 Glycosyltransferase from Mycobacterium avium
27% identity, 87% coverage
SS1G_04910 hypothetical protein from Sclerotinia sclerotiorum 1980 UF-70
33% identity, 32% coverage
Rv1524 Probable glycosyltransferase from Mycobacterium tuberculosis H37Rv
Mb1551 Probable glycosyltransferase from Mycobacterium bovis AF2122/97
26% identity, 95% coverage
- Identification of genes associated with persistence in Mycobacterium smegmatis
Joshi, Frontiers in microbiology 2024 - “...777 bp 6709869-6710645 Hypothetical protein No 214 L47 msmeg_0392 1515 bp 441687-443201 Putative glycosyltransferase No Rv1524 (58%) 686 L67 msmeg_6145 717 bp 6213505-6214221 Hypothetical protein No 114 S12 msmeg_3233 (cydA) 1464 bp 3316161-3317624 Cytochrome bd ubiquinol oxidase subunit I Yes Rv1623c (78%) 617 S14 msmeg_2211 840...”
- “...of action in the context of persistence. Additionally, the study of M. tuberculosis ortholog ( rv1524 ) may also reveal necessary attributes for the long-term survival of the pathogen. The msmeg_2263 gene is involved in molecular hydrogen (H 2 ) metabolism in mycobacteria ( Berney et...”
- Diversification of gene content in the Mycobacterium tuberculosis complex is determined by phylogenetic and ecological signatures
Silva-Pereira, Microbiology spectrum 2024 - “...narX (Rv1736c) is mostly pseudogenized in M. africanum L6 genomes when lipF (Rv3487c) or gtf3 (Rv1524) are intact, and vice versa ( Fig. 7 ; Table S3). This pattern is not observed in any other MTBC species. However, the gtf3 gene, a member of the GPL...”
- The rate and role of pseudogenes of the Mycobacterium tuberculosis complex
Soler-Camargo, Microbial genomics 2022 - “...ecotypes and is intact in M. canettii genomes. The putative glycosyltransferases 3 ( gtf3 ) Rv1524 and Rv1526c of the GPL locus are pseudogenized in 16 M . africanum L6 strains and in five M. bovis strains, respectively. The pks2 (Rv3825c), a gene of the sulfolipid...”
- “...carry lipF nor narX pseudogenes. In addition, strains of M. africanum L6 carrying the gtf3 (Rv1524) pseudogene have intact narX , while those carrying a narX pseudogene have an intact gtf3 . Thus, 15 M . africanum L6 strains carry both gtf3 and lipF pseudogenes. The...”
- Heat-killed Mycolicibacterium aurum Aogashima: An environmental nonpathogenic actinobacteria under development as a safe novel food ingredient
Nouioui, Food science & nutrition 2021 - “...and 66.1 <95% 0 gtf 1 orf01592 Rv1526c 48.8 <95% 1.3 gtf 2 orf01265; orf01577 Rv1524 58.2 and 53.8 <95% 9E162 mmp L10 orf01421 Rv1183 57.2 <95% 0 mmp S4 orf00497; orf04089 Rv0451c 47.5 <95% 3E47 mp s1 orf02234 Rv0101 49.3 <95% 0 pap A3 orf01420...”
- Orphan response regulator Rv3143 increases antibiotic sensitivity by regulating cell wall permeability in Mycobacterium smegmatis
Dong, Archives of biochemistry and biophysics 2020 (PubMed)- “...was screened out by surface plasmon resonance (SPR). Rv1524, the homologous gene of MSMEG_4740, belonging to the glycosyltransferase (Gtf) family, was related...”
- “...accumulation, we found when Rv3143 or MSMEG_4740, or Rv1524 was overexpressed, the cell wall permeability of Mycobacterium smegmatis was increased. In addition,...”
- Sources of Multidrug Resistance in Patients With Previous Isoniazid-Resistant Tuberculosis Identified Using Whole Genome Sequencing: A Longitudinal Cohort Study
Srinivasan, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2020 - “...2SRHZ/6HE Pt152 fabG1 C-15T rpsL K88R INH STR rpoB D435V (24 M>90%) RIF (24M) 4.5 Rv1524 (P344P) 2SRHZ/6HE Patients with intermediate SNPs difference Pt061 katG S315T embB M306I rpsL K43R INH STR rpoB S450L (2M=20%) RIF (2M) NADH pyrophosphatase nudC P239R (0M=80%, 2M>90%) 2.2 Rv2231c (A205A)...”
- “...Rv2907c [V113V]) 2.2.1 (12 M) Pt070 4.1.2 ( Rv0798c [L172L]) 2.2.1 (5M) Pt093 4.5 ( Rv1524 [P344P]) 2.2.1 (12M) Pt151 b 2.2.1 (1M) 2.2.1 (5M) Abbreviations: ID, identification number; M, month; MDR-TB, multidrug-resistant tuberculosis; Pt, patient; SNP, single-nucleotide polymorphism. a Initial indicates 0M, 1M, and 2M....”
- An integrated whole genome analysis of Mycobacterium tuberculosis reveals insights into relationship between its genome, transcriptome and methylome
Gomez-Gonzalez, Scientific reports 2019 - “...revealed as differentially expressed (Fig. 2 , Supplementary Table S2 ). Five of them ( Rv1524 - wbbL2 , Rv2652c - Rv2653c - Rv2658c ) correspond to known deletions in ancient isolates. PE_PGRS57 was also absent in ancient genomes of our samples, which has also been...”
- “...ancient (L5; M. Africanum ) strains in other studies 20 , 21 . As expected, Rv1524 - wbbL2 , Rv2652c - Rv2653c - Rv2658c and PE_PGRS57 transcripts were down-regulated in ancient strains. Forty-eight of the 105 (45.7%) genes found to be differentially expressed by clade have...”
- Mycobacterium marinum mmar_2318 and mmar_2319 are Responsible for Lipooligosaccharide Biosynthesis and Virulence Toward Dictyostelium
Chen, Frontiers in microbiology 2015 - “...mmar_2320 wecE , pyridoxal phosphate-dependent enzyme 10-A11 mmar_2323 Conserved hypothetical transmembrane protein 13-B8 mmar_2353 UDP-glycosyltransferase Rv1524 12-E11 Upstream of mmar_2684 PPE family protein PPE32 18-G4 mmar_3183 Hypothetical alanine rich protein 18-D7 Upstream of mmar_3375 Conserved hypothetical protein 17-A5, 18-H5, 12-A1 mmar_4263 Conserved hypothetical protein 13-G8 mmar_4621...”
- More
- Whole genome sequencing of the monomorphic pathogen Mycobacterium bovis reveals local differentiation of cattle clinical isolates
Lasserre, BMC genomics 2018 - “...genes (AdditionalFile 5 : Table S3). Among them we found pks12 and two glycosyltransferases ( Mb1551 and Mb1553c ) with identity scores between 86% and 93% in nine of the strains. Manual inspection of the alignment shows that several unequivocal mapped reads support these polymorphisms, evidencing...”
- “...AdditionalFile 10 : Table S6. The genes with low identity values mentioned before, pks12 , Mb1551 and Mb1553c , had the highest detected SNP density . Fig. 4 Visualization of the density of variants in the entire length of the reference genome (AF2122/97). Inner rays represent...”
ASPSYDRAFT_86678 uncharacterized protein from Aspergillus sydowii CBS 593.65
28% identity, 22% coverage
- Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1
Bu, BMC genomics 2020 - “...dihydroflavonol-4-reductase ASPSYDRAFT_162316 Reference-guided LDOX leucoanthocyanidin dioxygenase/anthocyanidin synthase ASPSYDRAFT_91437 Reference-guided UGTs_1 glycosyltransferase ASPSYDRAFT_33013 Reference-guided UGTs_2 glycosyltransferase ASPSYDRAFT_86678 Reference-guided UGTs_3 glycosyltransferase ASY002269 denovo UGTs_4 glycosyltransferase ASY000865 denovo OMT O-methytransferase Fig. 3 Statistics of gene functions related to anthocyanin metabolism in A. sydowii H-1 . ( A ) Putative...”
tylN / O70023 O-mycaminosyltylonolide 6-deoxyallosyltransferase (EC 2.4.1.317) from Streptomyces fradiae (see paper)
TYLN_STRFR / O70023 O-mycaminosyltylonolide 6-deoxyallosyltransferase; EC 2.4.1.317 from Streptomyces fradiae (Streptomyces roseoflavus) (see paper)
O70023 O-mycaminosyltylonolide 6-deoxyallosyltransferase (EC 2.4.1.317) from Streptomyces fradiae (see paper)
AAD12163.1 deoxyallosyl-transferase (TylN) (EC 2.4.1.-) (see protein)
tylN / CAA06512.2 deoxyallosyl-transferase from Streptomyces fradiae (see paper)
29% identity, 94% coverage
- function: Involved in the biosynthesis of the macrolide antibiotic tylosin derived from the polyketide lactone tylactone. Catalyzes the transfer of 6-deoxy-alpha-D-allose from dTDP-6-deoxy-alpha-D-allose to O-mycaminosyltylonolide (OMT) to yield demethyllactenocin.
catalytic activity: 5-O-beta-D-mycaminosyltylonolide + dTDP-6-deoxy-alpha-D-allose = demethyllactenocin + dTDP + H(+) (RHEA:15357)
disruption phenotype: Cells lacking this gene produce demycinosyl- tylosin.
UGT52_DICDI / Q54IL5 UDP-sugar-dependent glycosyltransferase 52; Sterol 3-beta-glucosyltransferase; UDP-glycosyltransferase 52; EC 2.4.1.173 from Dictyostelium discoideum (Social amoeba) (see paper)
Q54IL5 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Dictyostelium discoideum (see paper)
25% identity, 23% coverage
- function: Involved in the biosynthesis of sterol glucoside. Can use different sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors.
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
MSMEG_0392 hypothetical glycosyl transferase from Mycobacterium smegmatis str. MC2 155
26% identity, 79% coverage
- Editorial: Host-pathogen crosstalk: implications in host cellular processes by intracellular pathogens
Tripathi, Frontiers in microbiology 2024 - “...complex phenomenon involving various metabolic pathways and stress responses. Various loci msmeg_3233 (CydA), msmeg_0719, bioB, msmeg_0392 and msmeg_2263 (hybC) were identified to play critical roles in energy production, stress management, and survival strategies, highlighting their potential as targets for novel therapeutic interventions against persistent infections. The...”
- Identification of genes associated with persistence in Mycobacterium smegmatis
Joshi, Frontiers in microbiology 2024 - “...promoting long-term survival. Another group that displayed a moderate reduction in CFU included a glycosyltransferase, msmeg_0392 , a hydrogenase subunit, msmeg_2263 ( hybC ), and a DNA binding protein, msmeg_2211 . The study has revealed potential candidates likely to facilitate the long-term survival of M. smegmatis...”
- “...Flavohemoprotein No Rv0385 (68%) 319 L40 msmeg_6655 777 bp 6709869-6710645 Hypothetical protein No 214 L47 msmeg_0392 1515 bp 441687-443201 Putative glycosyltransferase No Rv1524 (58%) 686 L67 msmeg_6145 717 bp 6213505-6214221 Hypothetical protein No 114 S12 msmeg_3233 (cydA) 1464 bp 3316161-3317624 Cytochrome bd ubiquinol oxidase subunit I...”
- Insertion Mutation of MSMEG_0392 Play an Important Role in Resistance of M. smegmatis to Mycobacteriophage SWU1
Zhang, Infection and drug resistance 2022 - “...idr Infection and Drug Resistance 1178-6973 Dove 8818766 341494 10.2147/IDR.S341494 Original Research Insertion Mutation of MSMEG_0392 Play an Important Role in Resistance of M. smegmatis to Mycobacteriophage SWU1 Zhang et al Zhang et al Zhang Zhen 1 2 Yang Zhulan 3 Zhen Junfeng 1 Xiang Xiaohong...”
- “...strain to SWU1 was not observed. Through the amplification and sequencing of the target gene MSMEG_0392 , it was found that there was an adenine insertion mutation at position 817. After complementing MSMEG_0392 in M12, it was found that M12 returned to sensitivity to SWU1. Conclusion...”
- Weirdo19ES is a novel singleton mycobacteriophage that selects for glycolipid deficient phage-resistant M. smegmatis mutants
Suarez, PloS one 2020 - “...strains was performed according to Pavelka et al [ 14 ]. Amplification by PCR of MSMEG_0392 was carried out using primers MSMEG_0392Fw1 CCACGTCGTCGTCTTTCAG and MSMEG_0392Rev ACGAGTGGCGATGGACGAG under the following conditions: denaturation (94C, 5 min), followed by 30 cycles of denaturation (94C, 1 min), annealing (62C, 40...”
- “...upon analysis showed insertions in four genes [ 43 ]. We selected one of them, MSMEG_0392, encoding the glycosyltransferase gtf , as an initial candidate possibly underlying the phenotype of 1R-Weirdo19ES. PCR amplification and sequencing of this gene showed a single bp insertion at position 575...”
- The MprB extracytoplasmic domain negatively regulates activation of the Mycobacterium tuberculosis MprAB two-component system
Bretl, Journal of bacteriology 2014 - “...MSMEG_6080 MSMEG_1401 MSMEG_1424 MSMEG_6069 MSMEG_3155 MSMEG_0006 MSMEG_5415 MSMEG_0392 MSMEG_4671 rpoA rpoB ask mprB ndh kasA dnaJ qcrB pknA tuf lldD1 gyrA...”
- Identification of the polyketide synthase involved in the biosynthesis of the surface-exposed lipooligosaccharides in mycobacteria
Etienne, Journal of bacteriology 2009 - “...the GPL biosynthesis locus, MSMEG_0385, MSMEG_0389, and MSMEG_0392. Taken together, these observations strongly suggested that the cluster of genes downstream...”
MAB_4104 Putative glycosyltransferase GtfB from Mycobacterium abscessus ATCC 19977
35% identity, 42% coverage
MAB_4695c Putative glycosyltransferase/rhamnosyltransferase from Mycobacterium abscessus ATCC 19977
37% identity, 41% coverage
- Genetic diversification of persistent Mycobacterium abscessus within cystic fibrosis patients
Lewin, Virulence 2021 - “...MAB_4690c HFIHNKG_04663 lgrC_2 Probable non-ribosomal peptide synthetase PstA, Linear gramicidin synthase subunit C [Q] 6516-17 MAB_4695c HIFIHNKG_04668 Putative glycosyltransferase/rhamnosyltransferase [C] [G] 6516-20 MAB_4760 HIFIHNKG_04733 nox Probable nitroreductase [C] 10917-3 MAB_r5052 rrl 23S rRNA 10317-3, 10917-1 Clarithromycin [ 52 ] 1 accession: NC010397.1; 2 accession: ERS4791737; 3...”
BTH_II1076 rhamnosyltransferase I, subunit B from Burkholderia thailandensis E264
BTH_II1880 rhamnosyltransferase I, subunit B from Burkholderia thailandensis E264
31% identity, 82% coverage
C9YYI9 Putative glycosyltransferase from Streptomyces scabiei (strain 87.22)
33% identity, 82% coverage
FRAAL5400 Putative glycosyltransferase from Frankia alni ACN14a
31% identity, 45% coverage
- The Proteogenome of Symbiotic Frankia alni in Alnus glutinosa Nodules
Pujic, Microorganisms 2022 - “...proteins were overabundant such as glycosyl transferases (FRAAL2049, FC = 15.5; FRAAL4552, FC = 3.5; FRAAL5400, FC = 2.85), a sugar epimerase (FRAAL6795, FC = 10.67), a ribose isomerase (FRAAL1862, FC = 9.07), a sugar-binding protein (FRAAL5914, FC = 3.49) and an enolase (FRAAL6233, FC =...”
Afu2g02220 UDP-glucose:sterol glycosyltransferase from Aspergillus fumigatus Af293
26% identity, 29% coverage
MAB_4694c Glycosyltransferase from Mycobacterium abscessus ATCC 19977
25% identity, 89% coverage
AAM81359.1 UDP-Glc: sterol glycosyltransferase (Ugt51;Ugt51E1) (EC 2.4.1.173) (see protein)
27% identity, 24% coverage
ATG26_GIBZE / I1S8Q3 Sterol 3-beta-glucosyltransferase ATG26; Autophagy-related protein 26; EC 2.4.1.-; EC 2.4.1.173 from Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum) (see paper)
27% identity, 24% coverage
- function: Sterol glycosyltransferase responsible for the glycosylation of ergosterol to form ergosterol-glucoside.
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
catalytic activity: ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D- glucoside + UDP + H(+) (RHEA:61836)
disruption phenotype: Does not significantly decrease the growth rate under nutrient-rich conditions (PubMed:28894236).
ATG26_ASPOR / Q2U0C3 Sterol 3-beta-glucosyltransferase; Autophagy-related protein 26; EC 2.4.1.-; EC 2.4.1.173 from Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) (see paper)
25% identity, 29% coverage
- function: Sterol glycosyltransferase responsible for the glycosylation of ergosterol to form ergosterol-glucoside (By similarity). Involved in cytoplasm to vacuole transport (Cvt), pexophagy or nonselective autophagy (PubMed:27696999).
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
catalytic activity: ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D- glucoside + UDP + H(+) (RHEA:61836)
disruption phenotype: Leads to white colonies with decreased conidiation and aerial hyphae formation (PubMed:27696999). Impairs autophagy by the accumulation of the intermediate of autophagosome (PubMed:27696999).
Npun_R3449 glycosyl transferase family protein from Nostoc punctiforme
26% identity, 92% coverage
Q5B4C9 Sterol 3-beta-glucosyltransferase from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)
26% identity, 28% coverage
AAN77909.1 UDP-Glc: sterol glucosyltransferase (UGT53A1) (EC 2.4.1.173) (see protein)
25% identity, 26% coverage
An07g06610 uncharacterized protein from Aspergillus niger
27% identity, 27% coverage
WQ49_RS07360 glycosyltransferase from Burkholderia cenocepacia
BCAM2338 putative glycosyltransferase from Burkholderia cenocepacia J2315
31% identity, 88% coverage
- Identification of putative essential protein domains from high-density transposon insertion sequencing
Rahman, Scientific reports 2022 - “...family dehydratase MaoC-like dehydratase 46 0 New WQ49_RS04450 BCAM1749 Hypothetical protein Unknown 17 0 New WQ49_RS07360 BCAM2338 Glycosyl transferase family 1 UDP-glycosyltransferase 0 152 Domain (Glyco_transf_28) WQ49_RS07395 QU43_RS66100 Hypothetical protein Unknown 0 58 New WQ49_RS09185 BCAS0417 Cytochrome biogenesis protein CcdA Electron transfer 0 38 New WQ49_RS10495...”
- Identification of putative essential protein domains from high-density transposon insertion sequencing
Rahman, Scientific reports 2022 - “...dehydratase MaoC-like dehydratase 46 0 New WQ49_RS04450 BCAM1749 Hypothetical protein Unknown 17 0 New WQ49_RS07360 BCAM2338 Glycosyl transferase family 1 UDP-glycosyltransferase 0 152 Domain (Glyco_transf_28) WQ49_RS07395 QU43_RS66100 Hypothetical protein Unknown 0 58 New WQ49_RS09185 BCAS0417 Cytochrome biogenesis protein CcdA Electron transfer 0 38 New WQ49_RS10495 BCAS0158...”
- Comparative metabolic systems analysis of pathogenic Burkholderia
Bartell, Journal of bacteriology 2014 - “...rhlABC genes in B. ceno- 218 jb.asm.org cepacia are BCAM2340, BCAM2338, and BCAM2336, which have BLASTP E values of 7 1063, 5 1086, and 4 1063 to the...”
- Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction
Fang, BMC systems biology 2011 - “...61 ]. By protein similarity search against the UniProt database, proteins coded by genes BCAM2340, BCAM2338, and BCAM2336 in B. cenocepacia J2315 were identified as highly similar in sequence to rhlA, rhlB, and rhlC in both B. thailandensis (with BLAST E value of 1E-121, 1E-173, and...”
- “...identified from annotation as 93% similarity from Staphylococcus aureus and E value of 1E-33 BCAM2340, BCAM2338, BCAM2336 Putative (R)-3-hydroxydecanoyl-ACP: CoA transacylase, putative glycosyltransferase, putative sugar transferase Rhamnosyltransferase chain A, Rhamnosyltransferase chain B, Rhamnosyltransferase 2 RhlA, RhlB, RhlC - Strong physiological evidence from Dubeau et. al. (2009):...”
- Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment
O'Grady, Frontiers in cellular and infection microbiology 2011 - “...Putative acyl-CoA dehydrogenase 2.10 BCAM2237 Putative 2,2-dialkylglycine decarboxylase 2.41 BCAM2260 Major facilitator superfamily protein 1.61 BCAM2338 Putative glycosyltransferase 1.53 BCAM2356 Conserved hypothetical protein 1.63 d BCAM2453 Putative redoxin protein 1.69 BCAM2479 Putative transporter LysE family 1.54 BCAM2488 Putative phosphoglycerate/bisphosphoglycerate 1.56 BCAM2504 Conserved hypothetical protein 1.84 c...”
rhlB / Q51560 RhlB rhamnosyltransferase from Pseudomonas aeruginosa (see 4 papers)
Q51560 Rhamnosyl transferase from Pseudomonas aeruginosa
27% identity, 82% coverage
- Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14
Mahjoubi, Scientific reports 2021 - “...this group of compounds. Blastp searches against 3-(3-hydroxydecanoyloxy) decanoate synthase RhlA (Q51559), rhamnosyl transferase RhlB (Q51560) and Rhamnosyltransferase 2 RhlC (Q9I4K5) of P. aeruginosa did not yield orthologous of these proteins. The BUN14 proteome was subsequently annotated for glycosyltransferase (GT1 and GT2) signatures using dbCAN2 46...”
- “...to be a member of the glycosyltransferase family GT1. This protein shared 22.1% similarity with Q51560 (325 aa) of P. aeruginosa and the domain architecture of both proteins was similar. Seven BUN14 proteins were predicted to be members of the glycosyltransferase family GT2. Of these, two...”
CCM_01158 UDP-glucose:sterol glycosyltransferase from Cordyceps militaris CM01
28% identity, 24% coverage
D2EDM4 Rhamnosyltransferase-1 from Pseudomonas aeruginosa
27% identity, 83% coverage
AAG06866.1 rhamnosyltransferase (RhlB;PA3478) (EC 2.4.1.-) (see protein)
PA3478 rhamnosyltransferase chain B from Pseudomonas aeruginosa PAO1
NP_252168 rhamnosyltransferase subunit B from Pseudomonas aeruginosa PAO1
Q9HYD1 Rhamnosyltransferase chain B from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PA14_19110 rhamnosyltransferase chain B from Pseudomonas aeruginosa UCBPP-PA14
BWR11_07885 glycosyltransferase from Pseudomonas aeruginosa
27% identity, 82% coverage
- Inhibition of <i>Pseudomonas aeruginosa</i> quorum sensing by chemical induction of the MexEF-oprN efflux pump
Kristensen, Antimicrobial agents and chemotherapy 2024 - “...factors ( Table 1 ), such as genes encoding rhamnolipid production, rhlA (PA3479) and rhlB (PA3478), lasA (1871), and lasB (PA3724), which are involved in the production of elastase; the two phenazine operons phzA1-G1 (PA4210-PA4216) and phzA2-G2 (PA1899-PA1905), encoding phenazine biosynthesis proteins; phzM (PA4209), encoding a...”
- “...PA2570 lecA Lectin A Adhesion, cytotoxic 156 4.19E-86 PA3361 lecB Lectin B Adhesion 22 4.41E-75 PA3478 rhlB Rhamnolipid Immune evasion, motility 15 4.00E-77 PA3479 rhlA Rhamnolipid Immune evasion, motility 17 1.34E-58 PA3692 lptF Lipotoxin F Resistance to oxidative stress and adhesion -8 2.20E-21 PA3724 lasB Elastase...”
- Secondary metabolite profiling of Pseudomonas aeruginosa isolates reveals rare genomic traits
Neve, mSystems 2024 - “...described above, the biosynthetic pathway for di-RLs consists of a bicistronic operon of rhlAB ( PA3478 / PA3479 ) and the accessory gene rhlC ( PA1130 ) located separately on the genome ( 64 ). Analogously, the biosynthesis of the phenazine PCN is dependent upon the...”
- Mutation of Pseudomonas aeruginosa lasI/rhlI diminishes its cytotoxicity, oxidative stress, inflammation, and apoptosis on THP-1 macrophages
Ren, Microbiology spectrum 2024 - “...2-Heptyl-3-hydroxy-4(1H)-quinolone synthase Quorum sensing 1.64213425397 1.39715119297E-168 PA3479 rhlA Rhamnosyltransferase subunit A Quorum sensing 4.97060436644 0 PA3478 rhlB Rhamnosyltransferase subunit B Quorum sensing 4.58068784562 0 PA1130 rhlC Rhamnosyltransferase Quorum sensing 2.31958669047 2.29979491149E-250 PA1871 lasA Protease LasA Quorum sensing 3.73361327223 0 PA3724 lasB Elastase LasB Quorum sensing 1.39690736449...”
- Geraniol attenuates virulence factors by inhibiting quorum sensing of Pseudomonas aeruginosa
Li, Frontiers in microbiology 2023 - “...0.785649354 Quorum sensing PA2587 pqsH 0.054480343 0.906288652 Quorum sensing PA3479 rhlA 2.161753902 7.61774E-05 Quorum sensing PA3478 rhlB 1.879839834 4.79516E-05 Quorum sensing PA1130 rhlC 1.42583676 9.6008E-06 Quorum sensing PA1871 lasA 3.16297037 1.3179E-11 Quorum sensing PA3724 lasB 1.94856245 8.18372E-06 Quorum sensing PA2570 lecA 5.189666242 1.70617E-15 Quorum sensing PA3361...”
- A VirB4 ATPase of the mobile accessory genome orchestrates core genome-encoded features of physiology, metabolism, and virulence of Pseudomonas aeruginosa TBCF10839
Wiehlmann, Frontiers in cellular and infection microbiology 2023 - “...Acp3 77.9 PA3476 rhlL, autoinducer synthesis protein RhlL 10.7 PA3477 rhlR, transcriptional regulator RhlR 12.3 PA3478 rhlB, rhamnosyltransferase chain B 11.7 PA3479 rhlA, rhamnosyltransferase chain A 29.7 PA3519 Hypothetical protein 8.2 PA3535 Probable serine protease 9.1 PA3550 algF, alginate O -acetyltransferase AlgF 14.4 PA3690 Probable metal-transporting...”
- Inactivation of Pseudomonas aeruginosa biofilms by thymoquinone in combination with nisin
Chen, Frontiers in microbiology 2022 - “...thioesterase PqsE PA2587 1.40 pqsH quinolone synthase Virulence factors PA3479 5.73 rhlA rhamnosyltransferase subunit A PA3478 5.32 rhlB rhamnosyltransferase subunit B PA1130 3.04 rhlC rhamnosyltransferase PA1871 4.81 lasA protease LasA PA3724 5.38 lasB elastase LasB PA2570 5.88 lecA PA-I galactophilic lectin PA1077 3.14 flgB flagellar biosynthesis...”
- CRISPRi-Mediated Gene Suppression Reveals Putative Reverse Transcriptase Gene PA0715 to Be a Global Regulator of Pseudomonas aeruginosa
Zhou, Infection and drug resistance 2022 - “...biosynthesis outer 1.7047 Up Membrane protein PelC PA3063 pelB Pellicle/biofilm biosynthesis protein PelB 1.4383 Up PA3478 rhlB Rhamnosyltransferase subunit B 2.0555 Up PA3479 rhlA Rhamnosyltransferase subunit A 2.3069 Up PA3542 alg44 Alginate biosynthesis protein Alg44 1.4137 Up PA4969 cpdA cAMP phosphodiesterase 1.0595 Down Antibiotic resistance and...”
- Pf4 Phage Variant Infection Reduces Virulence-Associated Traits in Pseudomonas aeruginosa
Tortuel, Microbiology spectrum 2022 - “...precursor 62.50 PA3724 lasB* Elastase LasB AlgQ 40.00 14.49 Rhamnolipids PA1130 rhlC* Rhamnosyltransferase 2 10.87 PA3478 rhlB* Rhamnosyltransferase chain B AlgR, RhlR 22.22 PA3479 rhlA* Rhamnosyltransferase chain A AlgR, RhlR 15.87 6.25 Lectins PA2570 lecA* LecA RhlR 4.37 2.24 PA3361 lecB* Fucose-binding lectin PA-IIL AlgU, RhlR...”
- More
- The Pseudomonas aeruginosa rhlG and rhlAB genes are inversely regulated and RhlG is not required for rhamnolipid synthesis.
Bazire, BMC microbiology 2014 - GeneRIF: rhlG was inversely regulated compared to rhlAB and rhlC.
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - “...1192 Q9HT24 2274 G3XD48 3356 Q9HYC6 4438 Q9I306 111 Q9HZE0 1193 Q9HT32 2275 G3XD52 3357 Q9HYD1 4439 Q9I307 112 Q9HZJ2 1194 Q9HT33 2276 G3XD55 3358 Q9HYD4 4440 Q9I308 113 Q9HZK0 1195 Q9HT35 2277 G3XD56 3359 Q9HYD6 4441 Q9I309 114 Q9HZP8 1196 Q9HT36 2278 G3XD57 3360 Q9HYD7...”
- Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates
Jeske, NPJ biofilms and microbiomes 2022 - “...napB PA14_16250 lasB PA14_36820 PA14_49310 PA14_18630 eprS PA14_37745 PA14_51350 phnB PA14_19100 rhlA PA14_37760 PA14_51380 pqsE PA14_19110 rhlB PA14_37770 PA14_51390 pqsD PA14_19120 rhlR PA14_37780 PA14_51410 pqsC PA14_19130 rhlI PA14_38260 PA14_51420 pqsB PA14_19870 ldh PA14_39880 phzG2 PA14_51430 pqsA PA14_19900 PA14_39890 phzF2 PA14_53250 cpbD PA14_19910 pdhB PA14_39910 phzE2 PA14_55080...”
- Uracil influences quorum sensing and biofilm formation in Pseudomonas aeruginosa and fluorouracil is an antagonist
Ueda, Microbial biotechnology 2009 - “...genes upon deleting pyrF Quorum sensing PA14_19120 PA3477 rhlR 4.9 1.1 4.9 Transcriptional regulator RhlR PA14_19110 PA3478 rhlB 21.1 1.2 17.1 Rhamnosyltransferase chain B PA14_19100 PA3479 rhlA 19.7 1.4 16 Rhamnosyltransferase chain A PA14_45950 PA1431 rsaL 2.1 1.4 2.6 Regulatory protein RsaL PA14_40290 PA1871 lasA 39.4...”
- Pseudomonas aeruginosa L10: A Hydrocarbon-Degrading, Biosurfactant-Producing, and Plant-Growth-Promoting Endophytic Bacterium Isolated From a Reed (Phragmites australis)
Wu, Frontiers in microbiology 2018 - “...responsible for the biosurfactant synthesis. In the rhamnolipid pathway, the rhlAB genes ( BWR11_07880 and BWR11_07885 ; functioning as one operon) encode rhamnosyltransferase 1, while rhlR ( BWR11_07890 ) and rhlI ( BWR11_07895 ) are arranged in sequence and act as regulators of rhlAB expression (...”
K659_RS0103715 glycosyltransferase from Pseudomonas corrugata CFBP 5454
28% identity, 83% coverage
BLU14_RS07155 glycosyltransferase from Pseudomonas corrugata
28% identity, 83% coverage
ATG26_GLOLA / C4B4E5 Sterol 3-beta-glucosyltransferase; Autophagy-related protein 26; EC 2.4.1.-; EC 2.4.1.173 from Glomerella lagenarium (Anthracnose fungus) (Colletotrichum lagenarium) (see paper)
26% identity, 24% coverage
- function: Sterol glycosyltransferase responsible for the glycosylation of ergosterol to form ergosterol-glucoside (By similarity). Mediates autophagic degradation of peroxisomes (pexophagy) and is involved in pathogenesis via peroxisome degradation inside appressoria that are developing into the host invasion stage (PubMed:19363139).
catalytic activity: a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+) (RHEA:22724)
catalytic activity: ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D- glucoside + UDP + H(+) (RHEA:61836)
disruption phenotype: Leads to delayed autophagic degradation of peroxisomes in the appressoria (PubMed:19363139).
SPSK_04821 udp-transferase from Sporothrix schenckii 1099-18
29% identity, 19% coverage
- The Search for Cryptic L-Rhamnosyltransferases on the Sporothrix schenckii Genome
Mora-Montes, Journal of fungi (Basel, Switzerland) 2022 - “...SPSK_05538; 5-CAGAACGGAAAGACGGGACA-3 and 5-ACCACCGTTGTTGTTGACCT-3 for SPSK_05928; 5-AGGAATGACCATCAGCGCAA-3 and 5-AAACGCTCGGGAAAGTCCAT-3 for SPSK_01368; 5-AGCCACGAACGTCTCTTCAG-3 and 5-CTTCAAACGTGCCTGATGGC-3 for SPSK_04821; and 5-GTTTGTCCCCGACGTTTTGG-3 and 5-GCCGCGAGATGAAAAAGACG-3 for SPSK_01110. 2.7. Heterologous Gene Expression in Escherichia coli and Protein Purification The open reading frames under analysis were amplified by PCR using the primer pairs...”
- “...to search the S. schenckii proteome using this HMM profile, recovering five protein sequences (SPSK_01368, SPSK_04821, SPSK_05538, SPSK_05928, and SPSK_01110) ranging in sizes from 232 aa to 1836 aa ( Table 1 ). Four of the five sequences were automatically annotated as glycosyltransferases, and a fifth...”
HZ99_RS01145 glycosyltransferase from Pseudomonas fluorescens
27% identity, 92% coverage
ASPSYDRAFT_33013 uncharacterized protein from Aspergillus sydowii CBS 593.65
26% identity, 28% coverage
- Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1
Bu, BMC genomics 2020 - “...dihydroflavonol-4-reductase ASY001313 denovo DFR_5 dihydroflavonol-4-reductase ASPSYDRAFT_162316 Reference-guided LDOX leucoanthocyanidin dioxygenase/anthocyanidin synthase ASPSYDRAFT_91437 Reference-guided UGTs_1 glycosyltransferase ASPSYDRAFT_33013 Reference-guided UGTs_2 glycosyltransferase ASPSYDRAFT_86678 Reference-guided UGTs_3 glycosyltransferase ASY002269 denovo UGTs_4 glycosyltransferase ASY000865 denovo OMT O-methytransferase Fig. 3 Statistics of gene functions related to anthocyanin metabolism in A. sydowii H-1 ....”
MGG_03459 Atg26p from Pyricularia oryzae 70-15
26% identity, 22% coverage
MUL_1529 UDP-glycosyltransferase from Mycobacterium ulcerans Agy99
31% identity, 36% coverage
- Comparative phylogenomics of pathogenic and non-pathogenic mycobacterium
Prasanna, PloS one 2013 - “...of genes in response of MTB to acid shock [34] . Rv1524, ML2348, MMAR_2353, MAV_3258, MUL_1529, MAB_4112c. Glycosyl transferase Reaeration of Rv under hypoxia (2.753), 3 mM ACE (3.577), MEN (6.26) Glycosyltransferases are the enzymes that synthesizeoligosaccharides,and lipopoly-sachharides [45] . Rv3484, ML2247, MMAR_4966, MAV_0673, MUL_4040, MAB_0560....”
PSPA7_1648 rhamnosyltransferase chain B from Pseudomonas aeruginosa PA7
27% identity, 82% coverage
SS1G_07979 hypothetical protein from Sclerotinia sclerotiorum 1980 UF-70
26% identity, 24% coverage
SPSK_01368 sterol 3beta-glucosyltransferase from Sporothrix schenckii 1099-18
31% identity, 8% coverage
- The Search for Cryptic L-Rhamnosyltransferases on the Sporothrix schenckii Genome
Mora-Montes, Journal of fungi (Basel, Switzerland) 2022 - “...5- AACAGATTGAGGCTCTGGGC-3 and 5-AGTAAGTCTGGGTTCGCCAC-3 for SPSK_05538; 5-CAGAACGGAAAGACGGGACA-3 and 5-ACCACCGTTGTTGTTGACCT-3 for SPSK_05928; 5-AGGAATGACCATCAGCGCAA-3 and 5-AAACGCTCGGGAAAGTCCAT-3 for SPSK_01368; 5-AGCCACGAACGTCTCTTCAG-3 and 5-CTTCAAACGTGCCTGATGGC-3 for SPSK_04821; and 5-GTTTGTCCCCGACGTTTTGG-3 and 5-GCCGCGAGATGAAAAAGACG-3 for SPSK_01110. 2.7. Heterologous Gene Expression in Escherichia coli and Protein Purification The open reading frames under analysis were amplified by...”
- “...proceeded to search the S. schenckii proteome using this HMM profile, recovering five protein sequences (SPSK_01368, SPSK_04821, SPSK_05538, SPSK_05928, and SPSK_01110) ranging in sizes from 232 aa to 1836 aa ( Table 1 ). Four of the five sequences were automatically annotated as glycosyltransferases, and a...”
mycD / Q83WF1 mycinamicin VII 6-deoxyallosyltransferase from Micromonospora griseorubida (see 2 papers)
40% identity, 24% coverage
F4KII1 sterol 3beta-glucosyltransferase (EC 2.4.1.173) from Arabidopsis thaliana (see paper)
AT5G24750, NP_568452 UDP-Glycosyltransferase superfamily protein from Arabidopsis thaliana
NP_568452 hypothetical protein from Arabidopsis thaliana
26% identity, 34% coverage
- Identification of Genomic Loci and Candidate Genes Related to Seed Tocopherol Content in Soybean
Ghosh, Plants (Basel, Switzerland) 2022 - “...carried genomic variants of Chr08_14819413 and Chr15_43894622, respectively ( Table 4 ). Glyma.08G184700 (ortholog of AT5G24750) was annotated as UDP-Glycosyltransferase superfamily protein while Glyma.15G233300 (ortholog of AT1G55020) encodes a lipoxygenase 1. These two annotations (UDP-Glycosyltransferase superfamily protein and lipoxygenase 1) have been reported and may have...”
- Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis
Stucky, Journal of experimental botany 2015 - GeneRIF: Both UGT80A2 and UGT80B1, but not UGT713B1 were shown to be coordinately down-regulated during seed imbibition when SG levels decline, consistent with similar functions as UGT80 enzymes.
- “...third related enzyme, which seems specific to the plant lineage, is encoded by UGT713B1 / At5g24750 . Genetic and biochemical approaches were employed to determine the role of each UGT gene in the production of specific SGs and acyl SGs (ASGs). Using direct infusion electrospray ionization...”
- Deciphering the molecular functions of sterols in cellulose biosynthesis
Schrick, Frontiers in plant science 2012 - “...in planta . BLAST searches with UGT80 protein sequences reveal another candidate enzyme encoded by At5g24750, which has thus far not been characterized. Investigations of mutants corresponding to these and other candidate enzymes are expected to reveal the putative functions of SGs in cellulose biosynthesis. Concluding...”
- The interaction networks of small rubber particle proteins in the latex of Taraxacum koksaghyz reveal diverse functions in stress responses and secondary metabolism
Wolters, Frontiers in plant science 2024 - “...3- -glucosyltransferase UGT80B1 (NP_175027); AtUGT84A1, A. thaliana UDP-glycosyltransferase 84A1 (NP_193283.2); AtUGT713B1, A. thaliana glycosyltransferase UGT713B1 (NP_568452); CcUGT80B1, Cynara cardunculus var. scolymus sterol 3--glucosyltransferase UGT80B1 (XP_024976598.1); GhSGT1 B-like, Gossypium hirsutum sterol glucosyltransferase 1 homolog B-like (JN004107); HaUGT80B1, Helianthus annuus sterol 3--glucosyltransferase UGT80B1 (XP_035834958.1); HbUGT80B1-like isoform X1, H....”
- Tomato UDP-Glucose Sterol Glycosyltransferases: A Family of Developmental and Stress Regulated Genes that Encode Cytosolic and Membrane-Associated Forms of the Enzyme
Ramirez-Estrada, Frontiers in plant science 2017 - “...Analyses were conducted using MEGA version 7 ( Kumar et al., 2016 ). UDP-glucosyltransferases UGT713B1 (NP_568452), from Arabidopsis thaliana , and UGT713C3 (XP_004230516.1), from Solanum lycopersicum were used as outgroup. Functional Characterization of Tomato SGTs Expressed in E. coli In order to verify that the cloned...”
- Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis
Stucky, Journal of experimental botany 2015 - “...the EMBL/GenBank data libraries under accession numbers NP_566297 ( UGT80A2 ), NP_175027 ( UGT80B1 ), NP_568452 (Col-0 UGT713B1 ), KJ396595 (Ler UGT713B1 ) and NM_127546 ( GCS ). Supplementary Table S4 lists the accession numbers for the protein sequences in Fig. 1 . AGI locus identifiers:...”
W1PA48 Erythromycin biosynthesis protein CIII-like C-terminal domain-containing protein from Amborella trichopoda
26% identity, 33% coverage
- Exploring the Phytochemical Landscape of the Early-Diverging Flowering Plant Amborella trichopoda Baill
Wu, Molecules (Basel, Switzerland) 2019 - “...(484), W1NVU7 (388), W1NWE7 (342), W1NXD5 (462), W1P188 (462), W1P1M0 (474), W1P302 (465), W1P7W1 (486), W1PA48 (541), W1PAP0 (479), W1PAZ2 (518), W1PBB6 (485), W1PC48 (514), W1PD80 (476), W1PE57 (469), W1PF90 (490), W1PFP0 (486), W1PH20 (465), W1PHN8 (448), W1PJ19 (438), W1PKS4 (481), W1PLB6 (487), W1PLP3 (363), W1PMA9...”
AAF00215.1 (12b -derhodinosyl-)urdamycin G D-olivosyltransferase (UrdGT1b) (EC 2.4.1.-) (see protein)
45% identity, 22% coverage
FQZ25_19840 macrolide family glycosyltransferase from Bacillus thuringiensis
29% identity, 39% coverage
- A Genome-Centric Approach Reveals a Novel Glycosyltransferase from the GA A07 Strain of Bacillus thuringiensis Responsible for Catalyzing 15-O-Glycosylation of Ganoderic Acid A
Chang, International journal of molecular sciences 2019 - “...family genes were identified from the complete genome, among which three genes ( FQZ25_16345 , FQZ25_19840 , and FQZ25_19010 ) were closely related to BsUGT398 and BsUGT489. Two of the three candidate genes, FQZ25_16345 and FQZ25_19010 , were successfully cloned and expressed in a soluble form...”
- “...Among the 40 GTs, one GT1 ( FQZ25_19010 ) and two GT28 ( FQZ25_16345 , FQZ25_19840 ) family genes were most closely related to the five validated genes (marked by stars in Figure 4 ), and were considered putative gene candidates. 2.4. Cloning, Overexpression, and Purification...”
AAF00217.1 aquayamycin / urdamycinone B L-rhodinosyltransferase (UrdGT1c) (EC 2.4.1.-) (see protein)
43% identity, 24% coverage
AAS41737.1 flavonoid β-3(7)-O-glucosyltransferase (BcGT-3;BCE2825) (EC 2.4.1.-) (see protein)
21% identity, 95% coverage
FRAAL4787 putative N-glycosyltransferase from Frankia alni ACN14a
31% identity, 53% coverage
- Genomic Insights of Alnus-Infective Frankia Strains Reveal Unique Genetic Features and New Evidence on Their Host-Restricted Lifestyle
Kim, Genes 2023 - “...example, recovered from the list of lost genes in Sp+ genomes, such as FRAAL3448 or FRAAL4787 encoding a putative Glycerophosphoryl diester phosphodiesterase (indicated as GlpQ in Table 5 ) and putative N-glycosyltransferase, respectively. GlpQ is a protein able to hydrolyze glycerophosphodiester bonds [ 70 ] of...”
- “...FRAAL1769 Hypothetical protein FRAAL5611 Single copy C Energy production and conversion FRAAL1457 Putative Xanthine dehydrogenase FRAAL4787 Putative N-glycosyltransferase FRAAL3448 glpQ Glycerophosphoryl diester phosphodiesterase SP D Cell cycle control, cell division, chromosome partitioning FRAAL2959 ATP/GTP binding protein TM E Amino acid transport and metabolism FRAAL5354 Hypothetical protein...”
LOC112052352 uncharacterized protein LOC112052352 from Bicyclus anynana
27% identity, 14% coverage
- Transcriptional responses of Daphnis nerii larval midgut to oral infection by Daphnis nerii cypovirus-23
Kuang, Virology journal 2021 - “...hypothetical protein B5X24_HaOG201493 [Helicoverpa armigera] RDH12 TRINITY_DN9738_c0_g1_i1_6 438.41149 83.17535239 0.189719828 0.04256225 0.7412925 Down uncharacterized protein LOC112052352 [Bicyclus anynana] UGT TRINITY_DN8673_c0_g1_i3_3 839.7824168 167.2848772 0.199200262 0.00073535 0.0803495 Down PREDICTED: UDP-glucuronosyltransferase 2B19-like isoform X6 [Amyelois transitella] UGT TRINITY_DN17220_c0_g1_i1_4 6379.593263 3.929259199 0.000615911 0.00570705 0.2744919 Down UDP-glycosyltransferase UGT340C1 precursor [Bombyx mori]...”
J8Y18_13760 macrolide family glycosyltransferase from Bacillus cereus
28% identity, 39% coverage
- Investigating the Role of OrbF in Biofilm Biosynthesis and Regulation of Biofilm-Associated Genes in Bacillus cereus BC1
Sun, Foods (Basel, Switzerland) 2024 - “...IV subunit A ( J8Y18_17815 ), phosphoribosylamine-glycine ligase ThiC (J8Y18_25845 ), glycosyltransferase family I ( J8Y18_13760 ), adenosine succinate hydrolase ( J8Y18_01690 ), and UDP-N-acetylglucosamine lipid carrier transferase ( J8Y18_08880 ) ( Table 2 ). The gene encoding adenosine succinate hydrolase, J8Y18_01690 , plays a role...”
- “...NJS-5-10 J8Y18_17815 (2424) DNA topoisomerase IV subunit A NJS-5-26 J8Y18_25845 (1761) phosphomethylpyrimidine synthase ThiC NJS-5-26 J8Y18_13760 (1194) glycosyl transferase family 1 NJS-5-58 J8Y18_01690 (1308) adenylosuccinate lyase NJS-5-73 J8Y18_08880 (804) UDP-galactose-lipid carrier transferase...”
FSOA_HUMFU / P9WEH1 Terpene cyclase-glycosyl transferase fusion protein fsoA; Fuscoatroside biosynthesis cluster protein A; EC 5.4.99.-; EC 2.4.1.- from Humicola fuscoatra (see paper)
34% identity, 7% coverage
- function: Terpene cyclase-glycosyl transferase fusion protein; part of the gene cluster that mediates the biosynthesis of the enfumafungin- type antibiotic, fuscoatroside (PubMed:38654452). Within the pathway, fsoA plays two important roles, the cyclization of 2,3(S)-oxidosqualene into isomotiol via its terpene cyclase (TC) domain and the C3 glycosylation of several intermediates via its glycosyltransferase (GT) domain (PubMed:38654452). The fuscoatroside biosynthesis is initiated by the cyclization of 2,3(S)-oxidosqualene through FsoA's TC domain, leading to the formation of the fernane skeleton isomotiol, harboring a fernane triterpene skeleton with a C8-C9 double bond. Subsequently, C2- alpha-hydroxylation mediated by fsoD results in the production of 2- alpha-hydroxy-isomotiol, which is further acetylated by fsoF. The GT domain of FsoA may convert isomotiol, 2-alpha-hydroxy-isomotiol, and the acetylated derivative of 2-alpha-hydroxy-isomotiol into their corresponding glycosides 3-O-(beta-D-glucopyranosyl)-isomotiol, 3-O- (beta-D-glucopyranosyl)-2-alpha-hydroxy-isomotiol, and 3-O-(beta-D- glucopyranosyl)-2-alpha-acetoxy-isomotiol, which then undergo oxidative cleavage under the action of fsoE to form s 2-deacetoxy-fuscoatroside, 2-deacetyl-fuscoatroside, and fuscoatroside, respectively. Although hydroxylation followed by acetylation of 3-O-(beta-D-glucopyranosyl)- isomotiol and 2-deacetoxy-fuscoatroside by fsoD and fsoF could not be ruled out, this process is likely to occur with difficulty due to bulky steric hindrance caused by the presence of a glycan at C3 in these compounds. Interestingly, fsoE can also utilize the aglycones isomotiol and 2-alpha-hydroxy-isomotiol as substrates to generate 19-beta- hydroxy-isomotiol and 2-alpha,19-beta-dihydroxy-isomotiol, respectively. These reactions occur with lower efficiency. Finally, fsoE can further convert 2-alpha,19-beta-dihydroxy-isomotiol into 2- alpha-hydroxy-ismotiol-19-one and 2-alpha-hydroxy-ismotiol-19-one into 2-deacetyl-3-deglucopyranosyl-fuscoatroside (PubMed:38654452).
NDPGT_BACLD / Q65JC2 NDP-glycosyltransferase YjiC; UDP-glucosyltransferase YjiC; EC 2.4.1.384 from Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) (see 8 papers)
Q65JC2 flavone 7-O-beta-glucosyltransferase (EC 2.4.1.81) from Bacillus licheniformis (see paper)
AAU40842.1 UDP-Glc: isoflavonoid β-glucosyltransferase (YjiC;BLi01948;BL00446) (EC 2.4.1.-) (see protein)
32% identity, 39% coverage
- function: Glycosyltransferase that can glycosylate a wide range of substrates, including various flavonoids (flavones, flavonols, flavanones, flavanols, chalcones), isoflavonoids and stilbenes, to produce multiple glycosylated products (PubMed:23542617, PubMed:23974133, PubMed:24170092, PubMed:24893262, PubMed:24949266, PubMed:25239890, PubMed:27444326, PubMed:32238768). It can accept diverse nucleotide diphosphate-D/L-sugars as donors, including ADP-, GDP-, CDP-, TDP- or UDP-alpha-D-glucose, and catalyzes O-, N-, or S- glycosylation (PubMed:23542617, PubMed:23974133, PubMed:24170092, PubMed:24893262, PubMed:24949266, PubMed:25239890, PubMed:27444326, PubMed:32238768). In vitro, catalyzes the glycosylation of, among others, apigenin, 3-hydroxyflavone, phloretin or resveratrol, resulting in multiple glucosylated products, along with mono-, di-, tri- and tetraglucosides (PubMed:23542617, PubMed:23974133, PubMed:24170092, PubMed:24893262, PubMed:25239890, PubMed:27444326). Can also catalyze the glycosylation of the macrolide epothilone A with diverse NDP-D/L- sugars, forming different epothilone A glycoside derivatives (PubMed:24949266).
catalytic activity: an NDP-glycose + an acceptor = a glycosylated acceptor + NDP. - Polyphenol Utilization Proteins in the Human Gut Microbiome.
Zheng, Applied and environmental microbiology 2022 - “...Figure 3 and Fig. S1 showed that the 60 PUP seeds (except for I5AX49 and Q65JC2 ) contain in total 40 unique Pfam protein domains. According to a set of criteria (see Materials and Methods) and the sequence similarity network (SSN) analysis ( Fig. 3 ),...”
- “...Pfam domain or multidomain combination ( Table 1 ). The two PUPs ( I5AX49 and Q65JC2 ) without Pfam domains were defined as two unclassified families. Therefore, dbPUP contains in total 26 protein families of six enzymatic classes according to their enzyme commission (EC) numbers at...”
NDPGT_BACSU / O34539 NDP-glycosyltransferase YjiC; UDP-glycosyltransferase YjiC; EC 2.4.1.384 from Bacillus subtilis (strain 168) (see 3 papers)
O34539 NDP-glycosyltransferase (EC 2.4.1.384) from Bacillus subtilis (see 2 papers)
NP_389104 putative glycosyltransferase from Bacillus subtilis subsp. subtilis str. 168
34% identity, 26% coverage
- function: Glycosyltransferase that can glycosylate a wide range of substrates, including various flavonoids, phenyl ketones, curcuminoid, lignins, zingerone, triterpenes, stilbene and anthraquinone, using UDP- glucose or ADP-glucose as sugar donor (PubMed:28315700, PubMed:33152360). It also exhibits O-, N- and S-glycosylation activities towards simple aromatics (PubMed:28315700). In vivo, the broad acceptor tolerance of YjiC might function as a detoxification agent against exogenous xenobiotics to make the strain adaptable to the changeable environment (Probable).
catalytic activity: an NDP-glycose + an acceptor = a glycosylated acceptor + NDP.
subunit: Monomer. - Recent Advances in the Metabolic Engineering of Yeasts for Ginsenoside Biosynthesis
Chu, Frontiers in bioengineering and biotechnology 2020 - “...(AKQ76388), UGTPg1 (AIE12479), UGTPg101 (AKQ76389), UGTPg29 (AKA44579), UGTPg74AE2 (AGR44631), UGTPg45 (AKA44586), and B. subtilis YjiC (NP_389104), UGT109A1 (ASY97769). Subsequently, the condensation of IPP and DMAPP was catalyzed by farnesyl diphosphate synthase (FPS/ERG20) to form farnesyl diphosphate (FPP). Next, FPP was converted into 2,3-oxidosqualene through two enzymatic...”
- A Genome-Centric Approach Reveals a Novel Glycosyltransferase from the GA A07 Strain of Bacillus thuringiensis Responsible for Catalyzing 15-O-Glycosylation of Ganoderic Acid A
Chang, International journal of molecular sciences 2019 - “...microbial GTs were validated to have triterpenoid glycosylation activity, including BsYjiC (GenBank Protein accession no. NP_389104) from B. subtilis 168 [ 19 , 20 , 21 , 22 , 23 ], UGT109A1 (GenBank Protein accession no. ASY97769) from B. subtilis CTCG 63501 [ 24 , 28...”
- Uridine Diphosphate-Dependent Glycosyltransferases from Bacillus subtilis ATCC 6633 Catalyze the 15-O-Glycosylation of Ganoderic Acid A
Chang, International journal of molecular sciences 2018 - “...three microbial UGTs were validated with triterpenoid glycosylation activity, including BsYjiC (GenBank Protein Accession No. NP_389104) from B. subtilis 168 [ 19 , 20 ], UGT109A1 (GenBank Protein Accession No. ASY97769) from B. subtilis CTCG 63501 [ 21 ], and BsGT1 (GenBank Protein Accession No. ANP92054)...”
Celf_3212 glycosyltransferase from Cellulomonas fimi ATCC 484
41% identity, 21% coverage
- The genome sequences of Cellulomonas fimi and "Cellvibrio gilvus" reveal the cellulolytic strategies of two facultative anaerobes, transfer of "Cellvibrio gilvus" to the genus Cellulomonas, and proposal of Cellulomonas gilvus sp. nov
Christopherson, PloS one 2013 - “...PL3, and PL11 families in addition to four gene products involved in uronic acid interconversions (Celf_3212, _3268, _3292, and _3346) while C. flavigena had a PL3 and a PL11 pectate lyase and six predicted uronic acid gene products (Cfla_0976, _2984, _3012, _0879, _9878, _3194). In contrast,...”
AAD13559.1 olivosyltransferase (LanGT3) (EC 2.4.1.-) (see protein)
38% identity, 27% coverage
6kqxA / O34539 Crystal structure of yijc from b. Subtilis in complex with udp (see paper)
34% identity, 26% coverage
- Ligand: uridine-5'-diphosphate (6kqxA)
WP_003220489 glycosyltransferase from Bacillus spizizenii
31% identity, 32% coverage
7vlbA / A0A289QH46 Crystal structure of ugt109a1 from bacillus
31% identity, 32% coverage
- Ligand: uridine-5'-diphosphate (7vlbA)
7vlbB / A0A289QH46 Crystal structure of ugt109a1 from bacillus
31% identity, 32% coverage
- Ligand: uridine-5'-diphosphate (7vlbB)
SS1G_13524 hypothetical protein from Sclerotinia sclerotiorum 1980 UF-70
25% identity, 25% coverage
tylM2 / P95747 tylactone mycaminosyltransferase (EC 2.4.1.316) from Streptomyces fradiae (see paper)
TYLM2_STRFR / P95747 Tylactone mycaminosyltransferase; EC 2.4.1.316 from Streptomyces fradiae (Streptomyces roseoflavus) (see paper)
P95747 tylactone mycaminosyltransferase (EC 2.4.1.316) from Streptomyces fradiae (see paper)
CAA57472.2 TDP-D-mycaminose : tylactone mycaminyltransferase (TylMII;Orf2*) (EC 2.4.1.-) (see protein)
39% identity, 20% coverage
- function: Involved in the biosynthesis of the macrolide antibiotic tylosin derived from the polyketide lactone tylactone. Catalyzes the transfer of alpha-D-mycaminosyl from dTDP-alpha-D-mycaminose to the 5- hydroxyl group of tylactone to yield 5-O-mycaminosytylactone. It can also accept 16-membered tylactone and 12-membered ring macrolide.
catalytic activity: tylactone + dTDP-alpha-D-mycaminose = 5-O-beta-D- mycaminosyltylactone + dTDP + H(+) (RHEA:21468)
ABO27086.1 SaqAE3 (L-rhodinose) β-1,4A-olivosyltransferase (SaqGT3) (EC 2.4.1.-) (see protein)
39% identity, 17% coverage
eryBV / A4F7N6 dTDP-L-mycarosyl: erythronolide B mycarosyltransferase (EC 2.4.1.328) from Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) (see 2 papers)
ERYBV_SACER / O33939 Erythronolide mycarosyltransferase; EC 2.4.1.328 from Saccharopolyspora erythraea (Streptomyces erythraeus) (see paper)
SACE_0719 6-DEB TDP-mycarosyl glycosyltransferase from Saccharopolyspora erythraea NRRL 2338
32% identity, 33% coverage
- function: Involved in the biosynthesis of the macrolide antibiotic erythromycin. Catalyzes the reversible transfer of mycarosyl from dTDP- beta-L-mycarose to erythronolide B to yield 3-alpha-L- mycarosylerythronolide B. It can also use TDP-beta-L-cladinose.
catalytic activity: dTDP-beta-L-mycarose + erythronolide B = 3-O-alpha-L- mycarosylerythronolide B + dTDP + H(+) (RHEA:41576) - CRISPR/Cas9-Mediated Multi-Locus Promoter Engineering in ery Cluster to Improve Erythromycin Production in Saccharopolyspora erythraea
Zhang, Microorganisms 2023 - “...producer and six genes representing extremely low transcription levels, SACE_0716 , SACE_0717 , SACE_0718 , SACE_0719 , SACE_0720 , and SACE_0731 , encoding EryCIV, EryBVI, EryCVI, EryBV, EryBIV, and EryBIII, respectively, were characterized to play important roles in limiting erythromycin biosynthesis in S. erythraea NRRL 23338...”
- “...Limiting Genes In our previous study, six ery genes, SACE_0716 , SACE_0717 , SACE_0718 , SACE_0719 , SACE_0720 , and SACE_0731 , were demonstrated to play important roles in erythromycin biosynthesis, and overexpression of any one of the six genes led to improved erythromycin production in...”
- Droplet-Microfluidic-Based Promoter Engineering and Expression Fine-Tuning for Improved Erythromycin Production in Saccharopolyspora erythraea NRRL 23338
Yun, Frontiers in bioengineering and biotechnology 2022 - “...pSET152-hyg- p SACE_2101_s32 -SACE_0716, pSET152-hyg- p SACE_2101_s32 -SACE_0717, pSET152-hyg- p SACE_2101_s32 -SACE_0718, pSET152-hyg- p SACE_2101_s32 -SACE_0719, pSET152-hyg- p SACE_2101_s32 -SACE_0720, pSET152-hyg- p SACE_2101_s32 -SACE_0731, pSET152-hyg- p ermE*_s23 -SACE_0716, pSET152-hyg- p ermE*_s23 -SACE_0717, pSET152-hyg- p ermE*_s23 -SACE_0718, pSET152-hyg- p ermE*_s23 -SACE_0719, pSET152-hyg- p ermE*_s23 -SACE_0720, pSET152-hyg- p...”
- “.../pSET152-hyg- p SACE_2101_s32 -SACE_0717, S. erythraea /pSET152-hyg- p SACE_2101_s32 -SACE_0718, S. erythraea /pSET152-hyg- p SACE_2101_s32 -SACE_0719, S. erythraea /pSET152-hyg- p SACE_2101_s32 -SACE_0720, S. erythraea /pSET152-hyg- p SACE_2101_s32 -SACE_0731, S. erythraea /pSET152-hyg- p ermE*_s23 -SACE_0716, S. erythraea /pSET152-hyg- p ermE*_s23 -SACE_0717, S. erythraea /pSET152-hyg- p ermE*_s23 -SACE_0718,...”
3wagB / Q76KZ6 Crystal structure of glycosyltransferase vinc in complex with dtdp
36% identity, 27% coverage
- Ligand: thymidine-5'-diphosphate (3wagB)
BAD08357.1 dTDP-vicenisamine: vicenilactam β-vicenisaminyltransferase (VinC) (EC 2.4.1.-) (see protein)
36% identity, 26% coverage
SACE_4644 putative glycosyltransferase from Saccharopolyspora erythraea NRRL 2338
41% identity, 18% coverage
CAC37820.1 dTDP-D-desosamine: 3-α-mycarosylerythronolide B desosaminyltransferase (MegCIII) (EC 2.4.1.-) (see protein)
36% identity, 22% coverage
ERYC3_SACEN / A4F7P3 3-alpha-mycarosylerythronolide B desosaminyl transferase; Desosaminyl transferase EryCIII; Erythromycin biosynthesis protein CIII; EC 2.4.1.278 from Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) (see 3 papers)
CAA74710.1 TDP-desosamine: α-mycarosyl erythronolide B desosaminyltransferase (EryCIII;SACE_0726) (EC 2.4.1.-) (see protein)
YP_001102993 glycosyl transferase, NDP-D-desosamine : 3-L-mycarosyl erythronolide B from Saccharopolyspora erythraea NRRL 2338
33% identity, 23% coverage
- function: Catalyzes the conversion of alpha-L-mycarosylerythronolide B into erythromycin D in the erythromycin biosynthesis pathway.
catalytic activity: 3-O-alpha-L-mycarosylerythronolide B + dTDP-alpha-D-desosamine = erythromycin D + dTDP + H(+) (RHEA:32091)
subunit: Heterotetramer composed of EryCII and EryCIII. - Elucidation of the di-c-glycosylation steps during biosynthesis of the antitumor antibiotic, kidamycin.
Heo, Frontiers in bioengineering and biotechnology 2022 - “...[AknK (AAF70102), AknS (AAF73455), AraGT (ABL09968), Asm25 (AAM54103), DesVII (Q9ZGH7), ElaGT (ADP68587), EryBV (AAB84072), EryCIII (A4F7P3), GilGT (AAP69578), Gra-ORF14 (CAA09635), HedJ (AA85354), HedL (AAP85354), LanGT (AAD13562), LanGT2 (AAD13553), Med8 (BAC79040), SsfS6 (ADE34512), SunS (KIX81208), UrdGT2 (AAF00209) and VlnC (BAJ52701)] were aligned using the Clustal W method....”
- “...AknK (AAF70102), AknS (AAF73455), AraGT (ABL09968), Asm25 (AAM54103), DesVII (Q9ZGH7), ElaGT (ADP68587), EryBV (AAB84072), EryCIII (A4F7P3), GilGT (AAP69578), Gra-ORF14 (CAA09635), HedJ (AA85354), HedL (AAP85354), LanGT (AAD13562), LanGT2 (AAD13553), Med8 (BAC79040), SsfS6 (ADE34512), SunS (KIX81208), UrdGT2 (AAF00209), and VlnC (BAJ52701). Amino acid sequences were aligned using the...”
- Structural studies of the spinosyn forosaminyltransferase, SpnP
Isiorho, Biochemistry 2014 - “...substituted with other residues. The secondary structure is from SpnP. Accession numbers: SpnP, AAG23277; EryCIII, YP_001102993; MegCIII, CAC37820; DesVII, AAC68677; TylMII, CAA57472; CosG, ABC00729; DnrS, AAD15267; SnogD, AAF01811; SpnG, AAG23268; UrdGT2, AAF00209; CalG3, AAM94798; OleD, ABA42119; GtfA, AAB49292. (B) Stereodiagram of the three-helix motif showing the...”
KALB_6579 macrolide family glycosyltransferase from Kutzneria albida DSM 43870
34% identity, 27% coverage
LOC725997 UDP-glucuronosyltransferase 2C1 from Apis mellifera
25% identity, 33% coverage
YP_003204087 UDP-glucuronosyl/UDP-glucosyltransferase from Nakamurella multipartita DSM 44233
34% identity, 38% coverage
CAJ42338.1 steffimycin L-rhamnosyltransferase (StfG) (EC 2.4.1.-) (see protein)
stfG / CAJ42338.1 glycosyl transferase from Streptomyces steffisburgensis (see paper)
42% identity, 19% coverage
LOC118279357 UDP-glucosyltransferase 2-like from Spodoptera frugiperda
25% identity, 41% coverage
3otiA / Q8KND7 Crystal structure of calg3, calicheamicin glycostyltransferase, tdp and calicheamicin t0 bound form (see paper)
35% identity, 23% coverage
- Ligands: thymidine-5'-diphosphate; calicheamicin t0 (3otiA)
CHLREDRAFT_154976 uncharacterized protein from Chlamydomonas reinhardtii
34% identity, 15% coverage
T1KUK4 UDP-glycosyltransferase 202A2 from Tetranychus urticae
28% identity, 38% coverage
8sftB / T1KUK4 Crystal structure of tuugt202a2 (tetur22g00270) in complex with kaempferol
28% identity, 39% coverage
- Ligands: 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4h-chromen-4-one; uridine-5'-diphosphate (8sftB)
YP_138519 polyprotein from Cryphonectria hypovirus 4
33% identity, 3% coverage
mycB / Q83WE1 protomycinolide IV desosaminyltransferase from Micromonospora griseorubida (see 2 papers)
35% identity, 19% coverage
EFUA_HORCR / A0A2Z4HPY4 Enfumafungin synthase efuA; Enfumafungin biosynthesis cluster protein A; Terpene cyclase-glycosyl transferase fusion protein efuA; EC 5.4.99.-; EC 2.4.1.- from Hormonema carpetanum (see paper)
30% identity, 13% coverage
- function: Terpene cyclase-glycosyl transferase fusion protein; part of the gene cluster that mediates the biosynthesis of enfumafungin, a glycosylated fernene-type triterpenoid with potent antifungal activity, mediated by its interaction with beta-1,3-glucan synthase and the fungal cell wall (PubMed:30051576). The pathway begins with the terpene cyclase-glycosyl transferase fusion protein that most likely uses 2,3- oxidosqualene as substrate and catalyzes glycosylation immediately after cyclization (Probable). The fernene glycoside then could be processed by the desaturase efuI which catalyzes isomerization of a double bond established by efuA to form the core structure (Probable). The latter would then undergo a series of hydroxylations in unknown order at C-2, C-19, C-23 and C-25, which would be catalyzed by two of the three cytochrome P450 monooxygenases efuB, efuG or efuH (Probable). The hydroxy-group at C-25 becomes oxidized by the dehydrogenase efuE to enable a spontaneous, non-enzymatic hemiacetal formation with C-23 (Probable). After hydroxylation at C-2, acetylation by the acetyltransferase efuC takes place (Probable). The final steps in enfumafungin biosynthesis require expansion of the 5-membered ring by lactonization via a Baeyer-Villiger reaction mediated by one of the BGC's cytochrome P450 monooxygenases (efuB, efuG or efuH) followed by ring cleavage (Probable). This type of reaction would establish a double bond between C-20 and C-21 which could be reduced by the reductase efuL to form the final product (Probable).
disruption phenotype: Abolishes the production of enfumafungin and loses antifungal activity.
slr1125 zeaxanthin glucosyl transferase from Synechocystis sp. PCC 6803
28% identity, 38% coverage
ABL09968.1 TDP-L-Rha: CBS000020 α-L-rhamnosyltransferase (aranciamycin synthase) (AraGT;Orf21) (EC 2.4.1.-) (see protein)
46% identity, 12% coverage
SACE_3599 antibiotic resistance macrolide glycosyltransferase from Saccharopolyspora erythraea NRRL 2338
32% identity, 27% coverage
- Identification and Characterization of a Novel N- and O-Glycosyltransferase from Saccharopolyspora erythraea
Gutacker, Molecules (Basel, Switzerland) 2020 - “...to find the responsible glycosyltransferase, gene deletion experiments were performed and we identified the glycosyltransferase Sace_3599, which belongs to the CAZy family 1. When Streptomyces albus J1074, containing the dTDP- d -glucose synthase gene oleS and the plasmid pUWL-A- sace_3599 , was used as host, U3...”
- “...show homology to Uridine diphosphate (UDP)-glucosyltransferases and N -GTs of family 1 ( sace_1884 , sace_3599 , sace_4470 ). All these GT genes are not located close to biosynthetic gene clusters, but distributed throughout the genome. 2.3. Heterologous Expression of Sace_3599 in S. albus Gluc To...”
- Transcriptome-guided target identification of the TetR-like regulator SACE_5754 and engineered overproduction of erythromycin in Saccharopolyspora erythraea
Wu, Journal of biological engineering 2019 - “...the targets of SACE_5754, and confirmed that transcription of SACE_0388 (encoding a pyruvate, water diknase), SACE_3599 (encoding an antibiotic resistance macrolide glycosyltransferase) and SACE_6149 (encoding a FAD-binding monooxygenase) were directly repressed by SACE_5754. A consensus palindromic sequence TYMAGG-n2/n4/n11-KKTKRA (Y: C/T, M: A/C, K: T/G, R: A/G)...”
- “...and SACE_6148 were not affected by SACE_5754 deletion, but the transcriptional levels of SACE_0388 , SACE_3599 and SACE_6149 were increased by 158-, 4.7- and 95- folds, respectively (Fig. 3 d). It seemed that SACE_5754 directly represses the transcription of SACE_0388 , SACE_3599 and SACE_6149 . Fig....”
- Comparison of Antibiotic Resistance Mechanisms in Antibiotic-Producing and Pathogenic Bacteria
Ogawara, Molecules (Basel, Switzerland) 2019 - “...further rRNA methyltransferases are present in the genome. Furthermore, two putative macrolide glycosyltransferases (SACE_1884, and SACE_3599), and a number of efflux proteins for antibiotics exist outside of the cluster ( Table S1 ) [ 105 ]. These genes may be involved in the self-resistance. Oleandomycin is...”
AAS20331.1 β-olivosyltransferase (LndGT1) (EC 2.4.1.-) (see protein)
35% identity, 15% coverage
EGT_NPVAC / P18569 Ecdysteroid UDP-glucosyltransferase; EC 2.4.1.- from Autographa californica nuclear polyhedrosis virus (AcMNPV) (see paper)
AAA69845.1 UDP-Glc: ecdysteroid glucosyltransferase (Egt;UGT21A1) (EC 2.4.1.-) (see protein)
32% identity, 19% coverage
- function: Catalyzes the transfer of glucose from UDP-glucose to ecdysteroids which are insect molting hormones. Acts on the host at the organismal level to block its development, thereby increasing the yield of progeny virus.
cloM / Q8GHC2 L-demethylnoviosyl:clorobiocic acid transferase (EC 2.4.1.302) from Streptomyces roseochromogenus subsp. oscitans (see paper)
32% identity, 25% coverage
AAD13555.1 olivosyltransferase (LanGT1) (EC 2.4.1.-) (see protein)
32% identity, 19% coverage
NP_047420 UDP-Glucosyl Transferase from Bombyx mori nucleopolyhedrovirus
36% identity, 15% coverage
2iyaA / Q3HTL7 The crystal structure of macrolide glycosyltransferases: a blueprint for antibiotic engineering (see paper)
33% identity, 27% coverage
- Ligands: uridine-5'-diphosphate; (3s,5r,6s,7r,8r,11r,12s,13r,14s,15s)-6-hydroxy-5,7,8,11,13,15-hexamethyl-4,10-dioxo-14-{[3,4,6-trideoxy-3-(dimethylamino)-beta-d-xylo-hexopyranosyl]oxy}-1,9-dioxaspiro[2.13]hexadec-12-yl 2,6-dideoxy-3-o-methyl-alpha-l-arabino-hexopyranoside (2iyaA)
oleI / Q3HTL7 oleandomycin glycosyltransferase from Streptomyces antibioticus (see 3 papers)
ABA42118.2 oleandomycin glycosyltransferase (OleI) (EC 2.4.1.-) (see protein)
33% identity, 26% coverage
LRR80_00495 nucleotide disphospho-sugar-binding domain-containing protein from Streptomyces sp. RO-S4
47% identity, 12% coverage
- Integrated Metabolomic, Molecular Networking, and Genome Mining Analyses Uncover Novel Angucyclines From Streptomyces sp. RO-S4 Strain Isolated From Bejaia Bay, Algeria
Ouchene, Frontiers in microbiology 2022 - “...of sequence similarity to known enzymes involved in the modification of aromatic polyketides. Three genes (LRR80_00495, LRR80_00496, and LRR80_00498) likely associated with the glycosylation steps showed high similarity to genes coding for glycosyltransferases (GTs) in other angucyclines. All the annotated genes involved in the BGC of...”
- “...(Kawasaki et al., 2016 )], and SchS10 [81.63% (Basnet et al., 2006 )]. The second (LRR80_00495) is closely related to GcnG2 [92.46% (Zhang et al., 2013 )], sprGT2 [83.92% (Kawasaki et al., 2016 )], sqnG2 [81.91% (Salem et al., 2017 )], and schS9 [74.74% (Basnet et...”
CAC37814.1 mycarosyltransferase (MegBV) (EC 2.4.1.-) (see protein)
33% identity, 34% coverage
asm25 / Q8KUH5 ansamitocin N-glucosyltransferase from Actinosynnema pretiosum subsp. auranticum (see 4 papers)
AAM54103.1 ansamitocin N-β-glucosyltransferase (Asm25) (EC 2.4.1.-) (see protein)
30% identity, 44% coverage
UTI89_C1122 putative glucosyltransferase from Escherichia coli UTI89
O2ColV53 IroB from Escherichia coli
38% identity, 19% coverage
c1254 Putative glucosyltransferase from Escherichia coli CFT073
49% identity, 12% coverage
NRG857_30008 salmochelin biosynthesis C-glycosyltransferase IroB from Escherichia coli O83:H1 str. NRG 857C
38% identity, 19% coverage
iroB / A0A0H2V630 enterobactin C-glucosyltransferase (EC 2.4.1.369) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) (see 3 papers)
IROB_ECOL6 / A0A0H2V630 Enterobactin C-glucosyltransferase; Ent C-glucosyltransferase; EC 2.4.1.369 from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) (see 3 papers)
A0A0H2V630 enterobactin C-glucosyltransferase (EC 2.4.1.369) from Escherichia coli O6:H1 (see 2 papers)
38% identity, 19% coverage
- function: Catalyzes the successive monoglucosylation, diglucosylation and triglucosylation of enterobactin (Ent) (PubMed:15598734, PubMed:24960592). Transfers glucosyl groups from uridine-5'- diphosphoglucose (UDP-Glc) to C5 of one, two or three of the 2,3- dihydroxybenzoyl (DHB) units of Ent to yield monoglucosyl-C-Ent (MGE), diglucosyl-C-Ent (DGE) and triglucosyl-C-Ent (TGE) (PubMed:15598734, PubMed:24960592). Glucosylation decreases the membrane affinity of Ent and increases the iron acquisition rate (PubMed:17163637).
catalytic activity: enterobactin + UDP-alpha-D-glucose = monoglucosyl-enterobactin + UDP (RHEA:24448)
catalytic activity: monoglucosyl-enterobactin + UDP-alpha-D-glucose = diglucosyl- enterobactin + UDP + H(+) (RHEA:59096)
catalytic activity: diglucosyl-enterobactin + UDP-alpha-D-glucose = triglucosyl- enterobactin + UDP + H(+) (RHEA:59100)
AAC12648.1 UDP-Glc: oleandomycin β-glucosyltransferase (OleI) (EC 2.4.1.-) (see protein)
33% identity, 26% coverage
novM / Q9L9F5 4-O-demethyl-L-noviosyl transferase (EC 2.4.1.302) from Streptomyces niveus (see paper)
NOVM_STRNV / Q9L9F5 L-demethylnoviosyl transferase; Novobiocin biosynthesis protein M; EC 2.4.1.302 from Streptomyces niveus (Streptomyces spheroides) (see 3 papers)
32% identity, 26% coverage
- function: Catalyzes the transfer of L-noviose from dTDP-4-O-demethyl- beta-L-noviose to the phenolic oxygen of novobiocic acid, creating the full ABC ring system in the novobiocin biosynthesis pathway. Novobiocin is an aminocoumarin family antibiotic that targets bacterial DNA gyrases. Also shows activity with variant coumarin aglycones, suggesting it may be a promiscuous catalyst for noviosylation of a range of planar scaffolds. Does not show activity with TDP-L-rhamnose.
catalytic activity: dTDP-4-O-demethyl-beta-L-noviose + novobiocic acid = desmethyldescarbamoylnovobiocin + dTDP + H(+) (RHEA:36695)
t2668 putative glycosyl transferase from Salmonella enterica subsp. enterica serovar Typhi Ty2
38% identity, 21% coverage
RJF2_RS26160 salmochelin biosynthesis C-glycosyltransferase IroB from Klebsiella pneumoniae subsp. pneumoniae
40% identity, 19% coverage
ABB52547.1 TDP-D-chalcose: macrolide chalcosyltransferase / TDP-D-desosamine: macrolide D-desosaminyltransferase (GerTII) (EC 2.4.1.-) (see protein)
37% identity, 20% coverage
elmGT / Q9F2F9 8-demethyltetracenomycin C rhamnosyltransferase (EC 2.4.1.331) from Streptomyces olivaceus (see 2 papers)
ELMGT_STROV / Q9F2F9 Elloramycin glycosyltransferase ElmGT; EC 2.4.1.331 from Streptomyces olivaceus (see 2 papers)
Q9F2F9 8-demethyltetracenomycin C L-rhamnosyltransferase (EC 2.4.1.331) from Streptomyces olivaceus (see 5 papers)
CAC16413.2 dTDP-L-Rha: 8-demethyl-tetracenomycin C α-L-rhamnosyltransferase / elloramycin glycosyltransferase (ElmGT) (EC 2.4.1.-) (see protein)
elmgt / CAC16413.2 elloramycin glycosyltransferase from Streptomyces olivaceus (see 2 papers)
44% identity, 15% coverage
- function: Glycosyltransferase that transfers an L-rhamnose moiety from dTDP-L-rhamnose to the elloramycin aglycone 8-demethyl-tetracenomycin C (8DMTC) in elloramycin biosynthesis, an antitumor polyketide. Possesses donor substrate flexibility: able to transfer at least 11 different sugars to 8DMTC, such as NDP-D-glucose, as well as NDP-L-digitoxose, including both L- and D-isomeric forms of some sugars.
catalytic activity: 8-demethyltetracenomycin C + dTDP-beta-L-rhamnose = 8- demethyl-8-alpha-L-rhamnosyl-tetracenomycin C + dTDP + H(+) (RHEA:42848)
STM2773 putative glycosyl transferase, related to UDP-glucuronosyltransferase from Salmonella typhimurium LT2
47% identity, 12% coverage
- Comparative genomics between Colombian clinical isolates of Monophasic VariantSalmonellaTyphimurium and international clonal lineages
Paloma, 2023 - Evidence of international transmission of mobile colistin resistant monophasic Salmonella Typhimurium ST34
Supa-Amornkul, Scientific reports 2023 - “...was accompanied by an inversion of a 150,626-bp-long segment (position 2,916,0403,066,666), including 152 genes from STM2773 ( iroB ) to STM2924 ( rpoS ) and the last 111bp of STM2925c ( nlpD ). The inverted segment was flanked on both sides by IS 26 . There...”
- Identification of a Recently Dominant Sublineage in Salmonella 4,[5],12:i:- Sequence Type 34 Isolated From Food Animals in Japan
Arai, Frontiers in microbiology 2021 - “...and Pairwise Alignment of the DNA Sequences Nucleotide sequences of the genes from STM2743 to STM2773 in the Salmonella Typhimurium LT2 strain were used as query sequences. The detection thresholds were as follows: minimum coverage length 80% and nucleotide sequence identity 85%. Pairwise alignment of the...”
- Sequence Analyses and Phenotypic Characterization Revealed Multidrug Resistant Gene Insertions in the Genomic Region Encompassing Phase 2 Flagellin Encoding fljAB Genes in Monophasic Variant Salmonella enterica Serovar 4,5,12:i:- Isolates From Various Sources in Thailand
Win, Frontiers in microbiology 2021 - “...five target regions (131L, tniA , tetC , MAK, and 131R) spanning from STM2759 to STM2773 ( iroB ) among 108 Thai S . 4,5,12:i:- isolates and four non-Thai isolates (two Spanish and two U.S. strains) was performed. We identified nine patterns of presence or absence...”
- Population structure, case clusters, and genetic lesions associated with Canadian Salmonella 4,[5],12:i:- isolates
Clark, PloS one 2021 - “...of adjacent DNA, including fljAB hin , between homologs of S. Typhimurium homologs STM2692 and STM2773. A small number of isolates with this element are also found in other clades in which they do not predominate, raising questions about whether this prophage fragment is, in fact,...”
- Genotyping Study of Salmonella 4,[5],12:i:- Monophasic Variant of Serovar Typhimurium and Characterization of the Second-Phase Flagellar Deletion by Whole Genome Sequencing
Arrieta-Gisasola, Microorganisms 2020 - “...A1 ) fljAB 2 fljAB 2-A 1 1201 bp of STM2746 334 bp upstream from STM2773 ( iroB ) 820 bp (one IS26) fljAB 2-B 1 1263 bp of STM2746 fljAB 3 fljAB 3-A 1 177 of STM2753 334 bp upstream from STM2773 ( iroB )...”
- “...353 bp of STM2753 fljAB 4 3 222 bp downstream of STM2757 571 bp of STM2773 ( iroB ) 820 bp (one IS26) fljAB 5 1 222 bp downstream of STM2757 848 bp of the STM2784 820 bp (one IS26) fljAB 6 fljAB 6-A 1 1079...”
- Horizontal Acquisition of a Multidrug-Resistance Module (R-type ASSuT) Is Responsible for the Monophasic Phenotype in a Widespread Clone of Salmonella Serovar 4,[5],12:i:
García, Frontiers in microbiology 2016 - “...is inserted between two chromosomal loci, named STM2759 (encoding a putative phosphotransferase) and iro B (STM2773, a putative glycosyl transferase), causing the deletion of the genomic fragment located in between (15,716 bp). The latter comprises the genes STM2760STM2769 (encoding different putative proteins), the fljAB operon (encoding...”
- “...unknown function. The module is inserted between two chromosomal loci, named STM2759 and iro B (STM2773), causing the deletion of the genomic fragment located in between. Regarding the flanking chromosomal DNA of RR3, it shows high-level sequence identity to the corresponding regions of the biphasic S...”
- Characterization of Salmonella enterica serovar Typhimurium and Salmonella enterica serovar 4,[5],12:i:- isolates from pigs presenting with diarrhea in Korea
Lee, The Journal of veterinary medical science 2015 - “...25922 was used as a control strain. The expression of flagellar genes (STM1053-1997, STM2740, STM2757, STM2773, fljA , fljB and hin ) was assessed by PCR ( Table 1 ) as described previously [ 27 ]. DNA fragments were separated on a 1.5% agarose gel. Fragments...”
- More
3tsaB / Q9ALM8 Spinosyn rhamnosyltransferase spng (see paper)
38% identity, 25% coverage
- Ligand: alpha-d-glucopyranose (3tsaB)
spnG / Q9ALM8 spinosyn rhamnosyltransferase subunit from Saccharopolyspora spinosa (see paper)
AAG23268.1 TDP-β-L-Rha: spinosyn 9-O-α-L-rhamnosyltransferase (SpnG) (EC 2.4.1.-) (see protein)
38% identity, 25% coverage
megDI / Q9F839 dTDP-L-megosamine:erythromycin C L-megosaminyltransferase from Micromonospora megalomicea subsp. nigra (see 2 papers)
CAC37807.1 rhodosaminyltransferase (MegDI) (EC 2.4.1.-) (see protein)
34% identity, 22% coverage
ADU85989.1 tiacumicin 2-O-methyl-d-rhamnosyltransferase (TiaG2) (EC 2.4.1.-) (see protein)
30% identity, 34% coverage
YP_009051683 polyprotein from Phomopsis longicolla hypovirus
25% identity, 4% coverage
- Uncovering a Complex Virome Associated with the Cacao Pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata
Espinal, Pathogens (Basel, Switzerland) 2023 - “...(there are only six identified) viral species, namely, Cryphonectria hypovirus 3 (AAF13604), Phomopsis longicolla hypovirus (YP_009051683), Valsa ceratosperma hypovirus 1 (YP_005476604), Sclerotinia sclerotiorum hypovirus 1 (YP_004782527), Cryphonectria hypovirus 4 (YP_138519) and Setosphaeria turcica hypovirus 1 (AZT8861) ( Supplementary Figure S2 ). In addition to the previously...”
- Transfection of Sclerotinia sclerotiorum with in vitro transcripts of a naturally occurring interspecific recombinant of Sclerotinia sclerotiorum hypovirus 2 significantly reduces virulence of the fungus
Marzano, Journal of virology 2015 - “...CHV4 (YP_13851), FgHV1 (YP_00901106Pl), PlHV1 (YP_009051683), SsHV1 (YP_004782527), SsHV2/ 5472 (YP_008828161), SsHV2L (KF898354), SsHV2/sx247 (AIA61616), and...”
- “...YP_00901106Pl), Phomopsis longicolla hypovirus1 (PlHV1, YP_009051683), Sclerotinia sclerotiorum hypovirus 1 (SsHV1, YP_004782527), Sclerotinia sclerotiorum...”
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Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 798,070 different protein sequences to 1,261,478 scientific articles. Searches against EuropePMC were last performed on May 12 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory