PaperBLAST
PaperBLAST Hits for biolip::7cofA Cholesterol esterase from burkholderia stabilis (orthorhombic crystal form) () (320 a.a., ADDYATTRYP...)
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>biolip::7cofA Cholesterol esterase from burkholderia stabilis (orthorhombic crystal form) ()
ADDYATTRYPIVLVHGLTGTDKYAGVLEYWYGIQEDLQQHGATVYVANLSGFQSDDGPNG
RGEQLLAYVKTVLAATGATKVNLVGHSQGGLTSRYVAAVAPDLVASVTTIGTPHRGSEFA
DFVQGVLAYDPTGLSSSVIAAFVNVFGILTSSSHNTNQDALASLKTLTTAQAATYNQNYP
SAGLGAPGSCQTGAPTETVGGNTHLLYSWAGTAIQPTLSVFGVTGATDTSTIPLVDPANA
LDPSTLALFGTGTVMINRGSGQNDGLVSKCSALYGQVLSTSYKWNHIDEINQLLGVRGAF
AEDPVAVIRTHANRLKLAGV
Running BLASTp...
Found 104 similar proteins in the literature:
7cofA / A0A1Y1BQV9 Cholesterol esterase from burkholderia stabilis (orthorhombic crystal form)
100% identity, 100% coverage
- Ligand: calcium ion (7cofA)
Q75NT4 sterol esterase (EC 3.1.1.13) from Burkholderia cepacia (see paper)
96% identity, 88% coverage
- Efficient Preparation of High-Purity Fucoxanthinol by SpyTag-Tailored Active Cholesterol Esterase Aggregates.
Jin, Marine drugs 2022 - “...90.79%. 2.3. Preparation of Active Cholesterol Esterase Aggregates by SpyTag Tailoring We designed cholesterol esterase (Q75NT4) containing SpyTag. The fusion gene Pet-Q75NT4-SpyTag was transformed and induced, and SpyTag was terminally fused to Q75NT4. Figure 3 shows the diagrammatic sketch of the Pet-Q75NT4-SpyTag chimera design. Pet-Q75NT4-SpyTag was...”
- “...hydrolysis of cholesterol oleate ester and had cholesterol esterase activity, indicating that the target enzyme Q75NT4 was expressed in the form of active cholesterol esterase aggregates in Escherichia coli . Induction conditions were optimized on the basis of the yield of active cholesterol esterase aggregates. As...”
BCAM0949 exported lipase LipA from Burkholderia cenocepacia J2315
I35_RS20825 triacylglycerol lipase from Burkholderia cenocepacia H111
96% identity, 88% coverage
- Identification by Reverse Vaccinology of Three Virulence Factors in Burkholderia cenocepacia That May Represent Ideal Vaccine Antigens
Irudal, Vaccines 2023 - “...24 proteins. The localization and different aspects of virulence were investigated for three of themBCAL1524, BCAM0949, and BCAS0335. The three antigens were localized in the outer membrane vesicles confirming that they are surface exposed. We showed that BCAL1524, a collagen-like protein, promotes bacteria auto-aggregation and plays...”
- “...was obtained by growth in LB medium. To complement each deleted strain, BCAL1524 (1674 bp), BCAM0949 (1099 bp) and BCAS0335 (3595 bp) genes were amplified using B. cenocepacia K56-2 DNA as template and the primers pairs are listed in Table S3 . Fragments were cloned into...”
- Various Evolutionary Trajectories Lead to Loss of the Tobramycin-Potentiating Activity of the Quorum-Sensing Inhibitor Baicalin Hydrate in Burkholderia cenocepacia Biofilms
Sass, Antimicrobial agents and chemotherapy 2019 - “...same location (e.g., changes in BCAL1315, BCAL1664, and BCAM0949); we speculate that these mutations were already present in the starting population at low...”
- “...chemotaxis protein SNP in CDS (907087 G to T, A369C) 52 BCAM0949 Exported lipase LipA SNP in CDS (1051105 C to G, S180W) 47 100 100 100 100 100 100 100 100...”
- Phenotypic and genotypic characterisation of Burkholderia cenocepacia J2315 mutants affected in homoserine lactone and diffusible signal factor-based quorum sensing systems suggests interplay between both types of systems
Udine, PloS one 2013 - “...measured the expression of cepI (BCAM1870), cepR (BCAM1868), cciI (BCAM0239a), cciR (BCAM0240), zmpA (BCAS0409), lipA (BCAM0949), lipB (BCAM0950) and orbI (BCAL1696) by qPCR. Gene expression was measured in planktonic cells in the presence or absence of added signalling molecules. Although no difference in cepR gene expression...”
- Differential modulation of Burkholderia cenocepacia virulence and energy metabolism by the quorum-sensing signal BDSF and its synthase
Deng, Journal of bacteriology 2009 - “...zmpA (Bcas0409) encoding a metalloprotease (8, 20), lipA (Bcam0949) and lipB (Bcam0950) encoding a lipase and a lipase chaperone (14), respectively, and the...”
- “...increased the transcript levels of zmpA (Bcas0409), lipA (Bcam0949), lipB (Bcam0950), and orbI (Bcal1696) (Fig. 4A). We then tested whether BDSF can further...”
- Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia
O'Grady, BMC genomics 2009 - “...CciI NC 52.1 NC BCAM0240 N -acylhomoserine lactone dependent regulatory protein CciR NC 4.0 -23.5 BCAM0949 Exported lipase LipA -3.1 NC NC BCAM0950 Lipase chaperone LipB -2.5 NC NC BCAM1869 Conserved hypothetical protein -5.9 NC NC BCAM1871 Conserved hypothetical protein -37.6 NC -20.2 BCAM2307 Zinc metalloprotease...”
- “...medium and phase of growth influence regulation by CciR. Genes encoding the exported lipase LipA (BCAM0949) and the lipase chaperone LipB (BCAM0950, previously called limA ) are required for lipase production [ 32 ]. Expression of both lipA and lipB was decreased in the cepR mutant...”
- Transcriptional Response of Burkholderia cenocepacia H111 to Severe Zinc Starvation
Barnett, British journal of biomedical science 2023 - “...I35_RS10860 Imidazolonepropionase 2.326828435 0.022280782 I35_RS06165 Phenol degradation protein 2.320499563 2.27E28 I35_RS22340 Hypothetical protein 2.300982216 0.00000167 I35_RS20825 Alpha/beta hydrolase 2.246852811 0.035832439 I35_RS13380 ABC transporter permease 2.238628283 5.97E30 I35_RS10865 Hypothetical protein 2.099694274 0.022469745 I35_RS16595 DNA-binding protein 2.091617711 0.000362322 I35_RS29435 Hypothetical protein 2.072176035 0.022280782 I35_RS27035 2,2-Dialkylglycine decarboxylase 2.047152459 0.00024177...”
GB|BAA00960.1 triacylglycerol lipase; EC 3.1.1.3 from Pseudomonas sp. KWI-56 (see paper)
94% identity, 88% coverage
LIP_BURCE / P22088 Triacylglycerol lipase; Extracellular lipase; Triacylglycerol ester hydrolase; EC 3.1.1.3 from Burkholderia cepacia (Pseudomonas cepacia) (see 8 papers)
lipA / GI|557867 triacylglycerol lipase; EC 3.1.1.3 from Burkholderia cepacia (see 5 papers)
96% identity, 86% coverage
- function: Catalyzes the hydrolysis of triacylglycerol. It shows a preference for triacylglycerols with a chain length between 6 and 12 carbons.
catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
cofactor: Ca(2+) (Binds 1 Ca(2+) ion per subunit.)
subunit: Monomer. - A Novel Lipase from Streptomyces exfoliatus DSMZ 41693 for Biotechnological Applications
Rodríguez-Alonso, International journal of molecular sciences 2023 - “...tool of the BioEdit program and the amino acid sequence of Burkholderia cepacia lipase (UniProt P22088) as reference. Hypothetical genes encoding lipases from S. exfoliatus DSMZ 41693, including their signal peptide coding sequence ( lipA , lipB , lipC , and lipD ), were amplified by...”
- Comparative Structural Analysis of Different Mycobacteriophage-Derived Mycolylarabinogalactan Esterases (Lysin B).
Korany, Biomolecules 2019 - “...pancreatic lipase HuPL 1LPB P16233 8.6 2.8 147 465 15 Pseudomonas cepacia lipase PCL 1YS1 P22088 9.1 3.1 143 364 16 Candida rugosa lipase CRL 1Lpo P20261 7.3 3.4 100 549 12 1 Length according to UniprotKB. Note: Crystal structures were retrieved from Dali server. biomolecules-10-00045-t002_Table...”
A9QXC9 triacylglycerol lipase (EC 3.1.1.3) from Burkholderia cepacia (see paper)
94% identity, 87% coverage
BTH_II0639 lipase from Burkholderia thailandensis E264
89% identity, 88% coverage
BPSS1741 Lipase precursor from Burkholderia pseudomallei K96243
88% identity, 88% coverage
- Global transcriptional analysis of Burkholderia pseudomallei high and low biofilm producers reveals insights into biofilm production and virulence
Chin, BMC genomics 2015 - “...BPSS0486, BPSS0712, BPSS1285 and BPSS2328 ), seven phospholipases and lipase-related genes ( BPSL1064, BPSS0016, BPSS1740, BPSS1741, BPSS1937, BPSS2279 and BPSS2319 ) as well as seven cell envelope biogenesis-related genes ( BPSL0607, BPSL1872, BPSL3094, BPSL3312, BPSS1840, BPSS1932 and BPSS2016 ) were up-regulated in the high biofilm producer...”
LIP_PSEPS / P0DUB9 Triacylglycerol lipase; Extracellular lipase; Triacylglycerol ester hydrolase; EC 3.1.1.3 from Pseudarthrobacter phenanthrenivorans (Arthrobacter phenanthrenivorans) (see 2 papers)
83% identity, 100% coverage
- function: Catalyzes the hydrolysis of triacylglycerol.
catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
cofactor: Ca(2+) (Binds 1 Ca(2+) ion per subunit. {ECO:0000269|PubMed:8683577, ECO:0000305|Ref.)3}
subunit: Monomer (Probable). Interacts with lipase-specific foldase Lif (By similarity).
1tahB / P0DUB8 The crystal structure of triacylglycerol lipase from pseudomonas glumae reveals a partially redundant catalytic aspartate (see paper)
83% identity, 100% coverage
- Ligand: calcium ion (1tahB)
LIP_BURPL / P0DUB8 Triacylglycerol lipase; Extracellular lipase; Triacylglycerol ester hydrolase; EC 3.1.1.3 from Burkholderia plantarii (see 6 papers)
lipA / GB|CAA49812.1 Triacylglycerol lipase; EC 3.1.1.3 from Burkholderia glumae (see 4 papers)
84% identity, 88% coverage
- function: Catalyzes the hydrolysis of triacylglycerol.
catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
cofactor: Ca(2+) (Binds 1 Ca(2+) ion per subunit.)
subunit: Monomer (Probable). Interacts with lipase-specific foldase Lif (PubMed:16518399).
disruption phenotype: Loss of extracellular active lipase. Not required for growth on oleic acid as a carbon source.
BPSS2319 lipase precursor from Burkholderia pseudomallei K96243
71% identity, 90% coverage
- Global transcriptional analysis of Burkholderia pseudomallei high and low biofilm producers reveals insights into biofilm production and virulence
Chin, BMC genomics 2015 - “...BPSS2328 ), seven phospholipases and lipase-related genes ( BPSL1064, BPSS0016, BPSS1740, BPSS1741, BPSS1937, BPSS2279 and BPSS2319 ) as well as seven cell envelope biogenesis-related genes ( BPSL0607, BPSL1872, BPSL3094, BPSL3312, BPSS1840, BPSS1932 and BPSS2016 ) were up-regulated in the high biofilm producer (Figs. 2 and 3...”
WP_003239806 triacylglycerol lipase from Pseudomonas hydrolytica
45% identity, 99% coverage
- Insights into the biodegradation of polycaprolactone through genomic analysis of two plastic-degrading Rhodococcus bacteria
Zampolli, Frontiers in microbiology 2023 - “...al., 2015 ; Shi et al., 2020 ), BAI99230 (Hu et al., 2010 ), WP_004373894, WP_003239806 (Li et al., 2022 ), ADK73612 (Inglis et al., 2011 ), BAC67242 (Masaki et al., 2005 ), A0A0K8P6T7 (Yoshida et al., 2016 ), ADH43200 (Ribitsch et al., 2011 ), and...”
- “...2010 R3 WP_004373894 A0A8I0SEY2 JADMNJ010000005 MBF8162059 Hypothetical protein Pseudomonas mendocina Li et al., 2022 R4 WP_003239806 A0A8I0SFQ4 JADMNJ010000002 MBF8160636 Triacylglycerol lipase Pseudomonas mendocina Li et al., 2022 R5 ADK73612 E9KJL1 GU592443 ADK73612 Cutinase A precursor Pseudomonas oleovorans Inglis et al., 2011 R6 BAC67242 Q874E9 AB102945 BAC67242...”
FH974_07335 triacylglycerol lipase from Photobacterium ganghwense
45% identity, 99% coverage
H16_A1322 triacylglycerol lipase from Cupriavidus necator H16
H16_A1322 triacylglycerol lipase from Ralstonia eutropha H16
44% identity, 85% coverage
- Global changes in the proteome of Cupriavidus necator H16 during poly-(3-hydroxybutyrate) synthesis from various biodiesel by-product substrates
Sharma, AMB Express 2016 - “...chains from glycerol before they may be metabolized via the -oxidation pathway. Two lipase genes (H16_A1322 and H16_A3742) were identified previously in C. necator H16 transcriptomes from cells grown on palm oil (Brigham et al. 2010 ). However, neither of these proteins was present in the...”
- “...Most of the triglycerides were hydrolyzed in REG-GB and REG-FFA, therefore no induction of lipases (H16_A1322, H16_A1323) was observed. The -oxidation pathway provided the precursor for PHB synthesis when cultivated on REG-GB and REG-FFA. It appears that activities of -oxidation enzymes were maximum and no further...”
- Characterization of an extracellular lipase and its chaperone from Ralstonia eutropha H16
Lu, Applied microbiology and biotechnology 2013 (PubMed)- “...lipase (encoded by the lipA gene, locus tag H16_A1322) and lipase-specific chaperone (encoded by the lipB gene, locus tag H16_A1323) produced by R. eutropha...”
- “...H16 revealed two putative lipase genes (locus tags H16_A1322 and H16_A3742) that were upregulated during trioleate growth. Deletion of lipase H16_A1322 (GeneID,...”
VP1181 lactonizing lipase from Vibrio parahaemolyticus RIMD 2210633
VP_RS05740 triacylglycerol lipase from Vibrio parahaemolyticus RIMD 2210633
44% identity, 99% coverage
VCA0221 lactonizing lipase from Vibrio cholerae O1 biovar eltor str. N16961
46% identity, 94% coverage
LIP_VIBCH / P15493 Triacylglycerol lipase; Extracellular lipase; Triacylglycerol ester hydrolase; EC 3.1.1.3 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see 2 papers)
46% identity, 99% coverage
- function: Catalyzes the hydrolysis of triacylglycerol.
catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
cofactor: Ca(2+) (Binds 1 Ca(2+) ion per subunit.)
subunit: Monomer.
disruption phenotype: Cells lacking this gene do not show lipase activity.
MXAN_5522 lactonizing lipase from Myxococcus xanthus DK 1622
45% identity, 98% coverage
- Neutral and Phospholipids of the Myxococcus xanthus Lipodome during Fruiting Body Formation and Germination
Ahrendt, Applied and environmental microbiology 2015 - “...throughout the first 24 h of development. Similarly, MXAN_5522, encoding a lipase homolog involved in DAG and TAG degradation, was also downregulated. This...”
- “...development. In TAG metabolism, the downregulation of MXAN_5522, encoding a triacylglycerol lipase, might prevent the degradation of already formed TAGs....”
- Lipolytic enzymes in Myxococcus xanthus
Moraleda-Muñoz, Journal of bacteriology 2007 - “...encoded by MXAN_3852 contains motifs characteristic of patatins. MXAN_5522 encodes a protein with the G-X-S-X-G motif characteristic of the lipase subfamily of...”
- “...of lipolytic enzymes. Strains with deletions of MXAN_5522 and MXAN_4569 undergo faster development and earlier myxospore formation than the wild-type strain....”
PA14_63620 lipase LipC from Pseudomonas aeruginosa UCBPP-PA14
44% identity, 100% coverage
PA4813 lipase LipC from Pseudomonas aeruginosa PAO1
NP_253500 lipase LipC from Pseudomonas aeruginosa PAO1
44% identity, 100% coverage
- Pf4 Phage Variant Infection Reduces Virulence-Associated Traits in Pseudomonas aeruginosa
Tortuel, Microbiology spectrum 2022 - “...precursor 8.68 PA3910 eddA Extracelullar DNA degradation protein, EddA 3.91 PA4175 piv* Protease IV 29.41 PA4813 lipC Lipase LipC 6.38 Type 3 secretion system PA1690 pscU Translocation protein in type III secretion ExsA 7.95 PA1691 pscT Translocation protein in type III secretion ExsA 13.76 PA1692 pscS...”
- New Mouse Model for Chronic Infections by Gram-Negative Bacteria Enabling the Study of Anti-Infective Efficacy and Host-Microbe Interactions
Pletzer, mBio 2017 - “...length regulator Surface LPS 24.4 fimT (PA4549) Type 4 pilus biogenesis protein Adherence 17.9 lipC (PA4813) Lipase type II secretion system substrate Lipase C 13.2 lipA (PA2862) Major lipase type II secretion system substrate Lipase A 10.4 chpE (PA0417) Probable chemotaxis protein Chemotaxis 8.2 pchB (PA4230)...”
- The Pseudomonas aeruginosa PhoP-PhoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation
Gellatly, Infection and immunity 2012 - “...PA no. Gene Description PA3724 PA4175 PA1249 PA2862 PA2863 PA4813 PA0026 PA1092 PA4526 PA1178 lasB piv aprA lipA lipH lipC plcB fliC pilB oprH PA1179 PA3552...”
- Mucin promotes rapid surface motility in Pseudomonas aeruginosa
Yeung, mBio 2012 - “...1.4 2.2 0.8 PA3104 xcpP 7.1 1.3 8.9 2.5 PA3724 lasB 72.6 2.5 13.8 3.3 PA4813 lipC 5.1 1.5 3.6 1.4 Pyoverdine biosynthesis PA2385 pvdQ 5.1 1.3 7.7 2.4 PA2399 pvdD 3.3 1.1 6.2 2.1 PA2426 pvdS 6.1 1.6 5.7 2.1 Pyochelin biosynthesis PA4221 fptA 4.3...”
- The lipase LipA (PA2862) but not LipC (PA4813) from Pseudomonas aeruginosa influences regulation of pyoverdine production and expression of the sigma factor PvdS
Funken, Journal of bacteriology 2011 - “...20 The Lipase LipA (PA2862) but Not LipC (PA4813) from Pseudomonas aeruginosa Influences Regulation of Pyoverdine Production and Expression of the Sigma Factor...”
- “...33). Among those enzymes is the lipase LipC (PA4813), which was found to affect motility, biofilm formation, and rhamnolipid production (19), whereas the second...”
- Protein Secretion Systems in Pseudomonas aeruginosa: An Essay on Diversity, Evolution, and Function
Filloux, Frontiers in microbiology 2011 - “...et al. ( 1998 ) LipA (PA2862) Lipase Jaeger et al. ( 1994 ) LipC (PA4813) Lipase Martinez et al. ( 1999 ) LoxA (PA1169) Lipoxygenase Vance et al. ( 2004 ) PaAP (PA2939) Aminopeptidase Braun et al. ( 1998 ) PhoA (PA3296) Alkaline phosphatase Filloux...”
- Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection
Bohn, Molecular microbiology 2009 - “...to be involved in the control of the expression of the P. aeruginosa lipase LipC (PA4813) ( Martnez et al ., 1999 ). The PilY1 protein carries a prepilin peptidase export signal and is transported across the inner membrane ( Lewenza et al ., 2005 )....”
- Pseudomonas aeruginosa twitching motility-mediated chemotaxis towards phospholipids and fatty acids: specificity and metabolic requirements
Miller, Journal of bacteriology 2008 - “...PAO1 lipA (PA2862) extracellular lipase PAO1 lipC (PA4813) extracellular lipase PAO1 estA (PA5112) extracellular esterase/lipase PAO1 estA pBBX estA PAO1 fadL1...”
- “...PA3581 PA3582 PA3994 PA4148 PA4152 PA4198 PA4338 PA4589 PA4733 PA4813 PA5112 Genea 4042 MILLER ET AL. J. BACTERIOL. TABLE 2--Continued PA no. or function tar...”
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- Pressure-induced conformational switch of an interfacial protein.
Johnson, Proteins 2016 (PubMed)- GeneRIF: By monitoring the width of the access channel, this study found that the protein undergoes a conformational transition and opens the access channel at high hydrostatic pressures (>100 MPa).
- The lipase LipA (PA2862) but not LipC (PA4813) from Pseudomonas aeruginosa influences regulation of pyoverdine production and expression of the sigma factor PvdS.
Funken, Journal of bacteriology 2011 - GeneRIF: The LipA (PA2862) but not LipC (PA4813) influences regulation of pyoverdine production and expression of the sigma factor PvdS.
- Lipase LipC affects motility, biofilm formation and rhamnolipid production in Pseudomonas aeruginosa.
Rosenau, FEMS microbiology letters 2010 (PubMed)- GeneRIF: LipC is required for motility, biofilm formation and rhamnolipid production.
LIP_PSEAE / P26876 Triacylglycerol lipase; Extracellular lipase; Triacylglycerol ester hydrolase; EC 3.1.1.3 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 3 papers)
P26876 triacylglycerol lipase (EC 3.1.1.3) from Pseudomonas aeruginosa (see 2 papers)
NP_251552 lactonizing lipase from Pseudomonas aeruginosa PAO1
PA14_27100 lactonizing lipase precursor from Pseudomonas aeruginosa UCBPP-PA14
PA2862 lactonizing lipase precursor from Pseudomonas aeruginosa PAO1
41% identity, 100% coverage
- function: Catalyzes the hydrolysis of triacylglycerol (PubMed:1748875). It also exhibits some esterase activity with p-nitrophenyl acetate and Tween 80 as substrates, however the lipase activity is approximately eight times the esterase activity (PubMed:1748875). It shows a marked specificity for the 1,3-oleyl residues of triolein (PubMed:1748875).
catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
cofactor: Ca(2+) (Binds 1 Ca(2+) ion per subunit.)
subunit: Monomer. - Genome Wide Analysis of the Potato Soft Rot Pathogen Pectobacterium carotovorum Strain ICMP 5702 to Predict Novel Insights into Its Genetic Features.
Mallick, The plant pathology journal 2022 - “..., PA0688 P35482 Alkaline phosphatase L. Peg.3112 167 cp1 C5J5F5 Haloprotease CP1 Peg.2990 141 lipA P26876 Triacylglycerol lipase Peg.2760 401 lasA P14789 Protease LasA Peg.651 165 VC_A0027 Q9KND8 Chitinase Peg.1830 192 chiA P13656 Probable bifunctional chitinase/lysozyme Table 3 Protein encoding genes associated with type III secreted...”
- Bioinformatics Analysis of Metabolism Pathways of Archaeal Energy Reserves
Wang, Scientific reports 2019 - “...A: Diacylglycerol Acyltransferase 458 Q8GGG1 PF03007 PF06974 Pseudomonas aeruginosa B lip Lip Triacylglycerol lipase 311 P26876 PF00561 Wax Ester (WE) Acinetobacter baylyi B wax-dgaT WS/DGAT Wax Ester Synthase/Acyl Coenzyme A: Diacylglycerol Acyltransferase 458 Q8GGG1 PF03007PF06974 # D: three life domains: Archaea (A), Bacteria (B) and Eukaryote...”
- Evidence of a double-lid movement in Pseudomonas aeruginosa lipase: insights from molecular dynamics simulations
Cherukuvada, PLoS computational biology 2005 - “...http://www.rcsb.org/pdb/ ) accession number is 1EX9, and the Uniprot ( http://www.ebi.ac.uk/swissprot/access.html ) accession number is P26876. In appreciation of Prof. Varadachari Krishnan, JNCASR, Bangalore, India. We thank the Department of Biotechnology Government of India through the BioTechnology Information Services programme and the Department of Science and...”
- Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation.
Viegas, Scientific reports 2020 - GeneRIF: Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation.
- The Pseudomonas transcriptional regulator AlgR controls LipA expression via the noncoding RNA RsmZ in Pseudomonas protegens Pf-5.
Li, Biochemical and biophysical research communications 2017 (PubMed)- GeneRIF: In conclusion, this study for the first time indicates that AlgR directly binds to rsmZ to regulates the expression of lipA via regulating transcription of rsmZ, and mainly regulates the expression of lipA at transcriptional level in P. protegens Pf-5.
- The lipase LipA (PA2862) but not LipC (PA4813) from Pseudomonas aeruginosa influences regulation of pyoverdine production and expression of the sigma factor PvdS.
Funken, Journal of bacteriology 2011 - GeneRIF: The LipA (PA2862) but not LipC (PA4813) influences regulation of pyoverdine production and expression of the sigma factor PvdS.
- Mucin Glycans Signal through the Sensor Kinase RetS to Inhibit Virulence-Associated Traits in Pseudomonas aeruginosa
Wang, Current biology : CB 2021 - “...may upregulate the expression of genes associated with acute infections, including those that encode lipase (PA14_27100), rhamnolipids (PA14_1910019110), and the T3SS (PA14_4225042660). 21 , 36 However, unlike the H1-T6SS, the mechanism by which RetS controls these acute infection genes is unclear, because these transcripts are not...”
- Polysorbate 80 inhibition of Pseudomonas aeruginosa biofilm formation and its cleavage by the secreted lipase LipA
Toutain-Kidd, Antimicrobial agents and chemotherapy 2009 - “...The ligT gene is directly upstream of lipA (PA14_27100), which codes for an extracel- lular lipase. The lipases in bacteria have been extensively studied...”
- The periplasmic chaperone Skp prevents misfolding of the secretory lipase A from Pseudomonas aeruginosa
Papadopoulos, Frontiers in molecular biosciences 2022 - “...P. aeruginosa PAO1 strain were identified using Pseudomonas Genome database ( www.pseudomonas.com ). Those included PA2862 ( lipA ), PA2863 ( lipH ), PA3262 ( fkpA ), PA3801 ( yfgM ), PA 1805 ( ppiD ), PA0594 ( surA ) and PA3647 ( skp/ompH/hlpA ). The...”
- “...( Bernad et al., 2007 ; Tria et al., 2015 ). Lipase isolation The gene PA2862 encoding for the mature LipA was cloned into pET22b plasmid via NdeI/BamHI restriction sites ( Hausmann et al., 2008 ) and the protein was expressed in E. coli BL21(DE3). Briefly,...”
- Pf4 Phage Variant Infection Reduces Virulence-Associated Traits in Pseudomonas aeruginosa
Tortuel, Microbiology spectrum 2022 - “...4.81 PA0844 plcH Hemolytic phospholipase C precursor 2.94 PA0852 cbpD* Chitin-binding protein CbpD precursor 33.33 PA2862 lipA Lactonizing lipase precursor 2.02 PA2939 paaP* Probable aminopeptidase 50.00 PA3296 phoA Alkaline phosphatase 2.95 PA3319 plcN Nonhemolytic phospholipase C precursor 8.68 PA3910 eddA Extracelullar DNA degradation protein, EddA 3.91...”
- Putative RNA Ligase RtcB Affects the Switch between T6SS and T3SS in Pseudomonas aeruginosa
Dadashi, International journal of molecular sciences 2021 - “...6 4.76 10 5 1.2 PA1948 T2SS ToxA 5.76 10 6 4.91 10 5 1.1 PA2862 T2SS LipA 1.08 10 7 1.17 10 6 4.8 PA2939 T2SS PaAP 3.42 10 17 7.92 10 16 1.5 PA2676 T2SS HplS 0.010726 0.041385 1.8 PA2677 T2SS HplR 5.62 10...”
- A wide-ranging Pseudomonas aeruginosa PeptideAtlas build: A useful proteomic resource for a versatile pathogen
Reales-Calderón, Journal of proteomics 2021 - “...included other virulence factors, such as AprA (PA1249) [ 80 ] and the lipase LipA (PA2862) [ 81 ]. Thus, the analysis of the proteome under T3SS induction conditions has provided important contributions to the P. aeruginosa PeptideAtlas. Proteins involved in T3SS regulation, such as MexT...”
- Traditional Chinese Medicine Tanreqing Inhibits Quorum Sensing Systems in Pseudomonas aeruginosa
Yang, Frontiers in microbiology 2020 - “...hpcG 3.8 2-Oxo-hept3ene-1,7-dioate hydratase PA5351 rubA1 1.4 Rubredoxin 1 PA4670 prs 1.6 2.0 Ribose-phosphate pyrophosphokinase PA2862 lipA 1.8 2.7 Lactonizing lipase precursor PA3363 amiR 1.5 1.0 Aliphatic amidase regulator Central intermediary metabolism PA0654 speD 1.5 S -Adenosylmethionine decarboxylase proenzyme PA2393 1.6 Putative dipeptidase PA3182 pgl 1.8...”
- New Mouse Model for Chronic Infections by Gram-Negative Bacteria Enabling the Study of Anti-Infective Efficacy and Host-Microbe Interactions
Pletzer, mBio 2017 - “...protein Adherence 17.9 lipC (PA4813) Lipase type II secretion system substrate Lipase C 13.2 lipA (PA2862) Major lipase type II secretion system substrate Lipase A 10.4 chpE (PA0417) Probable chemotaxis protein Chemotaxis 8.2 pchB (PA4230) Pyochelin siderophore biosynthesis Iron uptake 8.2 exsA (PA1713) Type III secretion...”
- Characterization of the GbdR regulon in Pseudomonas aeruginosa
Hampel, Journal of bacteriology 2014 - “...(Table 3). We also noted that the lipA transcript (PA2862) was the only transcript whose abundance decreased under these conditions in a manner befitting a...”
- “...19.7 158.6 8.5 64.2 84.2 32.6 Transcript decreased in abundance PA2862 lipA 1.0 2.7 2.7 3.4 WT All changes are significant with P values of 0.00001. b PC,...”
- Regulatory and metabolic networks for the adaptation of Pseudomonas aeruginosa biofilms to urinary tract-like conditions
Tielen, PloS one 2013 - “...following primers were used: fur (PA4764) fur-fw `3-GAGGTGATCGAGTTCATGGATGC-5, fur-bw `3-GCACGTAGAGCACCAGATTGTGA-5, pvdS (PA2426) pvdS-fw`3-GATAACCGTACGATCCTGGTGAAGA-5, pvdS-bw`3-AGGTAGCTGAGCTGTGCCTTGAAC-5, lipA (PA2862) lipA-fw `3-CAGCACCTACACCCAGACCAAATAC -5, lipA-bw `3-GCTGACTTCGGTGACGTAGACCT-5, aprA (PA1249) aprA-fw `3-ATATCTACTCGCTGGGCAAGTTCAG-5, aprA-bw `3-GTCGACGAAGTGGATATTGGTGAC-5, lasB (PA3724) lasB-fw `3-GACCAACACCTACAAGCAGGTCAAC-5, lasB-bw `3-CTTCATGTACAGCTTGTGGGTCAG -5, narG (PA3875) narG-fw `3-TGAACGGCACCAGCTTCTTC-5, narG-bw `3-CGTTCGGCCTGGATGTTGTA-5. qRT-PCR was performed with SsoFast EvaGreen...”
- More
A8QYB2 triacylglycerol lipase (EC 3.1.1.3) from Pseudomonas aeruginosa (see paper)
41% identity, 100% coverage
1ex9A / P26876 Crystal structure of the pseudomonas aeruginosa lipase complexed with rc-(rp,sp)-1,2-dioctylcarbamoyl-glycero-3-o-octylphosphonate (see paper)
41% identity, 99% coverage
- Ligands: calcium ion; octyl-phosphinic acid 1,2-bis-octylcarbamoyloxy-ethyl ester (1ex9A)
Q9L6C7 Triacylglycerol acylhydrolase from Pseudomonas aeruginosa
41% identity, 100% coverage
LIP_PSEU0 / P26877 Triacylglycerol lipase; Extracellular lipase; Lactonizing lipase; Lipase P; Triacylglycerol ester hydrolase; EC 3.1.1.3 from Pseudomonas sp. (strain 109) (see paper)
41% identity, 100% coverage
- function: Catalyzes the hydrolysis of triacylglycerol. Also able to catalyze, in anhydrous organic solvents, intramolecular transesterification of omega-hydroxyfatty acid esters to form macrocyclic lactones. This biosynthesis is dependent on the chain length of the substrates, and the formation of monomer lactone is maximum with methyl 18-hydroxyoctadecanoate. With shorter substrates, monomer lactone decreases and the formation of diolide (dimer lactone) increases.
catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
cofactor: Ca(2+) (Binds 1 Ca(2+) ion per subunit.)
subunit: Monomer.
U876_RS20585 triacylglycerol lipase from Aeromonas hydrophila NJ-35
43% identity, 100% coverage
- In Vitro Antibiofilm Activity of Resveratrol against Aeromonas hydrophila
Qin, Antibiotics (Basel, Switzerland) 2023 - “...Extracellular Proteases U876_RS04035 1.86 2.85 Protease U876_RS18875 2.76 4.74 Elastase U876_RS20565 2.94 3.58 Collagenase Lipase U876_RS20585 1.66 2.34 Lipase U876_RS20590 1.68 1.93 Lipase chaperone T6SS U876_RS21275 1.47 - Type II/IV secretion system protein U876_RS13095 1.72 - Type VI secretion system baseplate subunit TssK U876_RS13100 1.73 -...”
MUW98_RS20835 triacylglycerol lipase from Aeromonas hydrophila
43% identity, 99% coverage
- The antivirulence activity, transcriptomics of EGCG and its protective effects on zebrafish infected by Aeromonas hydrophila
Yin, Frontiers in cellular and infection microbiology 2023 - “...Log2 Fold Change Description Genes associated with bacterial virulence MUW98_RS02610 -2.462476078 SGNH/GDSL hydrolase family protein MUW98_RS20835 -1.537520536 triacylglycerol lipase MUW98_RS04750 -2.189222336 methyl-accepting chemotaxis protein MUW98_RS09395 -1.877322408 methyl-accepting chemotaxis protein MUW98_RS19490 -1.749066889 methyl-accepting chemotaxis protein MUW98_RS11305 -1.427660893 methyl-accepting chemotaxis protein MUW98_RS20975 -1.17387116 HlyD family efflux transporter periplasmic...”
FUT48_16475 triacylglycerol lipase from Pseudomonas sp. JG-B
40% identity, 99% coverage
ACIAD3309 lipase from Acinetobacter sp. ADP1
40% identity, 98% coverage
- Engineering cell morphology by CRISPR interference in Acinetobacter baylyi ADP1
Luo, Microbial biotechnology 2022 - “...that it contains a kanamycin marker instead of a chloramphenicol marker and the sequences flanking ACIAD3309 instead of poxB . The integration vector pJL9 for acr1 ( ACIAD3383 ) knockout was constructed by cloning a chloramphenicol marker to the pUC57 plasmid containing the sequences flanking acr1...”
- “...2019 ) with pJL7; the gfp and mScarlet genes are integrated at the sites of ACIAD3309 and poxB respectively. Knockout of the rod shapedetermining genes, mreB and pbpA , was done by transformation with the linear cassettes constructed by assembling the corresponding left flanking sequence, a...”
- Prophage induction and differential RecA and UmuDAb transcriptome regulation in the DNA damage responses of Acinetobacter baumannii and Acinetobacter baylyi
Hare, PloS one 2014 - “...umuDAb 2.3 ACIAD1402 iscA Iron-binding protein umuDAb 2.3 ACIAD3506 aceF Dihydrolipoamide S -acetyltransferase umuDAb 2.2 ACIAD3309 Lipase umuDAb 2.2 ACIAD0316 htpG Heat shock protein; chaperone Hsp90 umuDAb 2.1 ACIAD3654 dnaK Heat shock protein; chaperone Hsp70 umuDAb 2.1 ACIAD0728 Conserved hypothetical protein umuDAb 2.1 ACIAD3564 Conserved hypothetical...”
- A trade-off between the fitness cost of functional integrases and long-term stability of integrons
Starikova, PLoS pathogens 2012 - “...acquired integrons on host fitness. The three integrons were inserted in an identical chromosomal locus (ACIAD3309) [32] . Mixed culture competition experiments revealed that newly acquired class-1 integrons from A. baumannii (IVS1 and IVS3) and S. enterica serovar Typhimurium (IVS2) resulted in a statistically significant reduced...”
- “...of the ancestor was by default set to 1.0. The neutrality of the insertion locus (ACIAD3309) was confirmed using a pair of A. baylyi ADP1 strains that were isogenic except from the insertion of a selective/counter-selective marker pair in strain IVS4 ( A. baylyi ADP1 ACIAD3309::...”
- Improved triacylglycerol production in Acinetobacter baylyi ADP1 by metabolic engineering
Santala, Microbial cell factories 2011 - “...(cytochrome) EC 1.2.2.2 i ACIAD3648 estA carboxylesterase EC 3.1.1.1 i,a ACIAD1134 aesT esterase - i,a ACIAD3309 M3 - lipase EC 3.1.1.3 a ACIAD1121 lip1 lipase EC:3.1.1.3 i,a Assessment and verification of in silico predictions According to the evaluation of the in silico model, gene annotation, and...”
- “...very different mechanisms affecting the TAG pathway were chosen to be studied experimentally. The gene ACIAD3309 is annotated as a TAG lipase degrading the product of interest. A strain lacking a fatty-acyl-CoA reductase ACIAD3383 ( acr1 ) is incapable of fatty alcohol biosynthesis and therefore wax...”
A1S_3160 lipase from Acinetobacter baumannii ATCC 17978
40% identity, 94% coverage
YE1842 lipase from Yersinia enterocolitica subsp. enterocolitica 8081
40% identity, 99% coverage
IV454_06595 triacylglycerol lipase from Massilia antarctica
39% identity, 100% coverage
LIP_PSEFR / P08658 Triacylglycerol lipase; Cold-adapted lipase; Extracellular lipase; Triacylglycerol ester hydrolase; EC 3.1.1.3 from Pseudomonas fragi (see 3 papers)
37% identity, 100% coverage
- function: Catalyzes the hydrolysis of triacylglycerols, with the highest activity with tributyrin (C4), lower activity with tricaprylin (C8), and much lower activity with triacetin (C2), trilaurin (C12) and triolein (C18).
catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
cofactor: Ca(2+) (Binds 1 Ca(2+) ion per subunit.)
PMI0999 lipase from Proteus mirabilis HI4320
WP_004247922 triacylglycerol lipase from Proteus mirabilis
39% identity, 97% coverage
4hs9A / B4EVM3 Methanol tolerant mutant of the proteus mirabilis lipase (see paper)
38% identity, 97% coverage
- Ligand: calcium ion (4hs9A)
PL78_18430 triacylglycerol lipase from Yersinia entomophaga
39% identity, 97% coverage
WP_015093259 triacylglycerol lipase from Pseudomonas sp. UW4
38% identity, 98% coverage
- Heterologous expression and characterization of a new lipase from Pseudomonas fluorescens Pf0-1 and used for biodiesel production
Liu, Scientific reports 2017 - “.... fluorescens (identity: 92.6%); AEV60646, a triacylglycerol lipase from P . fluorescens F113 (identity: 82.1%); WP_015093259, an lipase from Pseudomonas sp. UW4 (identity: 85.5%); CAA32193, a unnamed protein product from P . fragi (identity: 43.9%); CAC07191, a lipase from P . fragi (identity: 47.1%), which indicates...”
- “...lipase from P . fluorescens ; AEV60646, a triacylglycerol lipase from P . fluorescens F113; WP_015093259, an lipase from Pseudomonas sp . UW4; CAA32193, a unnamed protein product from P . fragi ; CAC07191, a lipase from P . fragi . Cluster 1.83 and ESpript 2.0...”
PFLU0569 lipase precursor from Pseudomonas fluorescens SBW25
39% identity, 98% coverage
Pfl01_0571 Alpha/beta hydrolase fold from Pseudomonas fluorescens Pf0-1
39% identity, 99% coverage
EY04_02420 triacylglycerol lipase from Pseudomonas chlororaphis
38% identity, 98% coverage
- Polymer-Degrading Enzymes of Pseudomonas chloroaphis PA23 Display Broad Substrate Preferences
Mohanan, International journal of molecular sciences 2023 - “...PA23 genome ( Table 1 ). Three of these encode intracellular lipases (EY04_08410, EY04_09635, and EY04_02420), while the other three contain a signal peptide indicative of possible extracellular activity (EY04_21540, EY04_17885, and EY04_32435). Curiously, only one encodes an intracellular PHA depolymerase (EY04_01535). In our initial experiments...”
- “...MD, USA. 4.4. Sequence Analysis and Homology Modeling Amino acid sequence similarity searches for LIP3 (EY04_02420), LIP4 (EY04_21540), and PhaZ (EY04_01535) of P. chlororaphis PA23 were carried out using Basic Local Alignment Search Tool (BLAST) for proteins ( http://blast.ncbi.nlm.nih.gov/Blast.cgi (accessed on 1 July 2021)). The sequences...”
- Characterization of Polymer Degrading Lipases, LIP1 and LIP2 From Pseudomonas chlororaphis PA23
Mohanan, Frontiers in bioengineering and biotechnology 2022 - “...as esterase/lipase ( Table 3 ). Of these, three encode for intracellular lipases (EY04_08410, EY04_09635, EY04_02420), three encode a signal peptide and are extracellular lipases (EY04_21540, EY04_17885, EY04_32435), and one encodes for an intracellular PHA depolymerase (EY04_01535). In our previous study, the hydrolysis of the ester...”
- “...weight (kDa) LIP1 EY04_08410 Intracellular 828 275 29.7 LIP2 EY04_09635 Intracellular 834 277 30.2 LIP3 EY04_02420 Intracellular 891 296 32.5 LIP4 EY04_21540 Extracellular 942 315 34.0 LIP5 EY04_17885 Extracellular 1905 634 69.8 LIP6 EY04_32435 Extracellular 1911 636 70 Lipase genes cloned into the pET28a(+) vector and...”
PSF113_0610 triacylglycerol lipase from Pseudomonas ogarae
39% identity, 99% coverage
- Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea
Trantas, Frontiers in microbiology 2015 - “...PcorDraft_04315-04305 PmedDraft_01591-01607 PSF113_0437-0447 Putative substrates PcorDraft_04316 PcorDraft_03149 PcorDraft_03269 PcorDraft_02349 PmedDraft_01594 PmedDraft_05894 PmedDraft_04897 PmedDraft_05316 PmedDraft_00632 PSF113_0435, PSF113_0610, PSF113_2337, PSF113_3279 Hxc PcorDraft_00325-00347 PmedDraft_04580-04583 PSF113_3690-3700 T3SS SPI-I not detected in Pcor strains not detected in Pmed strains PSF113_1778-1801 Hrp1 not detected in Pcor strains not detected in Pmed strains...”
- Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction
Redondo-Nieto, BMC genomics 2013 - “...(PSF113_2948), lipA (PSF113_2944) PSF113_3005-3007 psmE (PSF113_3004) PSF113_3055-3056* PSF113_3303-3305 T2SS Xcp xcpP-Z (PSF113_0437-0447), xcpA (PSF113_5006) PSF113_0435, PSF113_0610, PSF113_2337, PSF113_3279 Hxc hxcP-Z ( PSF113_3690-3700) psp (PSF113_3701) T3SS SPI-I invF - orgB (PSF113_1778-1801) PSF113_1802 Hrp1 rspS - rspL (PSF113_5585-5610) ropAA-1 (PSF113_1126), ropAA-2 (PSF113_3486), ropB (PSF113_5598), ropM (PSF113_5616) T4SS GI-like...”
- “...it seems that at least four predicted F113 proteins could potentially be Xcp substrates (PSF113_0435, PSF113_0610, PSF113_2337 and PSF113_3279). T3SSs T3SS is a nanomachine composed of approximately 25 proteins [ 61 ], which has evolved into seven different families: Ysc, Hrp1, Hrp2, SPI-1, SPI-2, Rhizobiaceae and...”
WP_011058985 triacylglycerol lipase from Pseudomonas protegens
PFL_0617 lipase from Pseudomonas fluorescens Pf-5
37% identity, 98% coverage
Q88DH1 Lipase from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
PP_4854 lipase from Pseudomonas putida KT2440
36% identity, 98% coverage
PSF113_3279 triacylglycerol lipase from Pseudomonas ogarae
36% identity, 99% coverage
- Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea
Trantas, Frontiers in microbiology 2015 - “...PSF113_0437-0447 Putative substrates PcorDraft_04316 PcorDraft_03149 PcorDraft_03269 PcorDraft_02349 PmedDraft_01594 PmedDraft_05894 PmedDraft_04897 PmedDraft_05316 PmedDraft_00632 PSF113_0435, PSF113_0610, PSF113_2337, PSF113_3279 Hxc PcorDraft_00325-00347 PmedDraft_04580-04583 PSF113_3690-3700 T3SS SPI-I not detected in Pcor strains not detected in Pmed strains PSF113_1778-1801 Hrp1 not detected in Pcor strains not detected in Pmed strains PSF113_5585-5610 T4SS...”
- Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction
Redondo-Nieto, BMC genomics 2013 - “...(PSF113_2944) PSF113_3005-3007 psmE (PSF113_3004) PSF113_3055-3056* PSF113_3303-3305 T2SS Xcp xcpP-Z (PSF113_0437-0447), xcpA (PSF113_5006) PSF113_0435, PSF113_0610, PSF113_2337, PSF113_3279 Hxc hxcP-Z ( PSF113_3690-3700) psp (PSF113_3701) T3SS SPI-I invF - orgB (PSF113_1778-1801) PSF113_1802 Hrp1 rspS - rspL (PSF113_5585-5610) ropAA-1 (PSF113_1126), ropAA-2 (PSF113_3486), ropB (PSF113_5598), ropM (PSF113_5616) T4SS GI-like PSF113_3314-3334 T5aSS...”
- “...at least four predicted F113 proteins could potentially be Xcp substrates (PSF113_0435, PSF113_0610, PSF113_2337 and PSF113_3279). T3SSs T3SS is a nanomachine composed of approximately 25 proteins [ 61 ], which has evolved into seven different families: Ysc, Hrp1, Hrp2, SPI-1, SPI-2, Rhizobiaceae and Chamydia [ 62...”
LEPBI_I0886 lactonizing lipase (triacylglycerol lipase) from Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)'
36% identity, 54% coverage
LIC_12988 alpha/beta fold hydrolase from Leptospira interrogans serovar Copenhageni str. Fiocruz LV130
LIC12988 lipase from Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
34% identity, 54% coverage
- A comprehensive in silico analysis of putative outer membrane and secretory hydrolases from the pathogenic Leptospira: Possible implications in pathogenesis
Shankar, Biotechnology and applied biochemistry 2024 (PubMed)- “...generate a deeper understanding. Our analysis yielded four putative outer/secretory hydrolases, LIC_10995, LIC_11183, LIC_11463, and LIC_12988, containing / hydrolase fold and displayed similarity with lipase motif. Moreover, their conservation analysis of the predicted hydrolases across the spectrum of different Leptospira species showed high clustering with the...”
- Extracellular Proteome Analysis Shows the Abundance of Histidine Kinase Sensor Protein, DNA Helicase, Putative Lipoprotein Containing Peptidase M75 Domain and Peptidase C39 Domain Protein in Leptospira interrogans Grown in EMJH Medium
Sarma, Pathogens (Basel, Switzerland) 2021 - “...Ex. S 8 WP_000416054.1 LIC_11345 TonB-dependent siderophore receptor - - 0.04 Ex. S 9 WP_000738678.1 LIC_12988 lipase - - 14.3 Ex. S 10 WP_000812418.1 LIC_10645 hypothetical protein - - 1.4 Ex. S 11 WP_000844347.1 LIC_10346 SGNH/GDSL hydrolase family protein - - 1.2 Ex. S 12 WP_000899352.1...”
- Genome-wide subcellular localization of putative outer membrane and extracellular proteins in Leptospira interrogans serovar Lai genome using bioinformatics approaches
Viratyosin, BMC genomics 2008 - “...the consensus vote Lai Locus Copen Locus Protein annotation LA3731 LIC10497 Fmh-like protein/hypothetical protein LA0587 LIC12988 Lactonizing lipase/lipase LA0872 LIC12760 Microbial collagenase LA1450 LIC12302 Probable O-sialoglycoprotein endopeptidase LA2448 LIC10830 Putative outermembrane protein/putative lipoprotein LA1765 LIC12047 Rhs family protein/cytoplasmic membrane protein LA4161 LIC13320 Thermolysin/thermolysin precursor LA4164 LIC13321...”
TGL2_YEAST / P54857 Triacylglycerol lipase 2; Lipase 2; Neutral lipid hydrolase; EC 3.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
NP_010343, YDR058C Protein with lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli; role in yeast lipid degradation is unclear from Saccharomyces cerevisiae
NP_010343 triglyceride lipase from Saccharomyces cerevisiae S288C
32% identity, 37% coverage
- function: Mitochondrial triacylglycerol (TAG) lipase with activity toward long-chain diacylglycerols (DAGs) and triacylglycerols (TAGs) (PubMed:19959834, PubMed:9544243). Involved in mitochondrial lipid metabolism (PubMed:31483742).
catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
catalytic activity: 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)- octadecenoylglycerol + (9Z)-octadecenoate + H(+) (RHEA:38575)
catalytic activity: 1,2,3-tributanoylglycerol + H2O = dibutanoylglycerol + butanoate + H(+) (RHEA:40475)
catalytic activity: 1,2,3-trioctanoylglycerol + H2O = dioctanoylglycerol + octanoate + H(+) (RHEA:47864)
catalytic activity: di-(9Z)-octadecenoylglycerol + H2O = (9Z-octadecenoyl)- glycerol + (9Z)-octadecenoate + H(+) (RHEA:47868)
catalytic activity: dioctanoylglycerol + H2O = octanoylglycerol + octanoate + H(+) (RHEA:47880)
subunit: Interacts with MIA40; forms mixed disulfide intermediates with MIA40. - High-throughput biochemical fingerprinting of Saccharomyces cerevisiae by Fourier transform infrared spectroscopy
Kohler, PloS one 2015 - “...RSB1 44 WT WT BY4743 5 YOR100C CRC1 45 YJR019C TES1 6 YOR171C LCB4 46 YDR058C TGL2 7 YOR196C LIP5 47 1 YJL196C ELO1 8 YOR245C DGA1 48 1 , 2 YKR053C YSR3 9 YOR377W ATF1 49 1 YCR048W ARE1 10 1 , 2 YOL002C IZH2...”
- “...GC. ORF Gene sample # YHR067W HTD2 1 a , b WT WT BY4743 4 YDR058C TGL2 7 YKR067W GPT2 12 YLR450W HMG2 13 YJR073C OPI3 19 a , b YJR103W URA8 20 YKL140W TGL1 22 YLL012W YEH1 24 YML008C ERG6 31 a , b YMR205C...”
- Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae
Michaillat, PloS one 2013 - “...1.8 YOL011W PLB3 Phospholipase B 1.5 YOR081C TGL5 Triacylglycerol lipase preferring VLCFAs; acyltransferase activity 1.8 YDR058C TGL2 Acylglycerol lipase 1.5 YIL040W APQ12 Unknown; mutant accumulates triglycerides 2.0 YOR084W LPX1 Putative lipase 1.5 YDR503C LPP1 Lipid phosphate phosphatase 1.0 YDL052C SLC1 Lyso-PA acyl transferase 1.3 YNR008W LRO1...”
- Gene cloning and characterization of a novel esterase from activated sludge metagenome
Zhang, Microbial cell factories 2009 - “...the putative lipase/esterase from Magnaporthe grisea 70-15 and Saccharomyces cerevisiase Tg12p (XP_368471, 31% identity; and NP_010343, 29% identity, respectively), members of the family of fungal hydrolases. And also, the EstAS contained a catalytic triad (Ser92, His125, and Asp249) and a LHYFRG conserved motif (starting from His36),...”
- “...Server http://www.ch.embnet.org/software/BOX_form.html . XP_504639, esterase/lipase from Yarrowia lipolytica CLIB122; XP_368471, LipA from Magnaporthe grisea 70-15; NP_010343, esterase/lipase from Saccharomyces cerevisiae Tg12p; ZP_02733109, lipase from Gemmata obscuriglobus UQM 2246. Figure 2 Phylogenetic analysis of EstAS and closely related proteins . Phylogenetic analysis was performed using the program...”
- The yeast protein kinase C cell integrity pathway mediates tolerance to the antifungal drug caspofungin through activation of Slt2p mitogen-activated protein kinase signaling
Reinoso-Martín, Eukaryotic cell 2003 - “...c YLR017w YDR444w YDR461w YHR096c YAL042w YAR071w YDR058c YBR145w YCL009c YGL009c YDL182w YBR272c YBR014c YBR153w YJR024c Function ER-associated protein...”
- The mitochondrial intermembrane space-facing proteins Mcp2 and Tgl2 are involved in yeast lipid metabolism.
Odendall, Molecular biology of the cell 2019 - GeneRIF: Data show that mitochondrial protein MCP2 (MCP2) has a negative genetic interaction with the gene triglyceride lipase TGL2 (TGL2) encoding a neutral lipid hydrolase.
- The TGL2 gene of Saccharomyces cerevisiae encodes an active acylglycerol lipase located in the mitochondria.
Ham, The Journal of biological chemistry 2010 - GeneRIF: mitochondrial Tgl2p-dependent lipolysis is crucial for the survival of cells under antimitotic drug treatment
ZP_02733109 Esterase/lipase/thioesterase family active site from Gemmata obscuriglobus UQM 2246
26% identity, 98% coverage
- Gene cloning and characterization of a novel esterase from activated sludge metagenome
Zhang, Microbial cell factories 2009 - “...it showed 33% and 31% amino acid identity to esterase/lipase from Gemmata obscuriglobus UQM 2246 (ZP_02733109) and Yarrowia lipolytica CLIB122 (XP_504639), respectively; and several conserved regions were identified, including the putative active site, HSMGG, a catalytic triad (Ser92, His125 and Asp216) and a LHYFRG conserved motif....”
- “...quite low identity with other esterase/lipases, highest with the esterase/lipase from Gemmata obscuriglobus UQM 2246 (ZP_02733109, 33% identity), followed by the lipase from Yarrowia lipolytica CLIB122 (XP_504639, 31% identity), the putative lipase/esterase from Magnaporthe grisea 70-15 and Saccharomyces cerevisiase Tg12p (XP_368471, 31% identity; and NP_010343, 29%...”
XP_504639 YALI0E31515p from Yarrowia lipolytica CLIB122
38% identity, 37% coverage
- Novel lipolytic enzymes identified from metagenomic library of deep-sea sediment
Jeon, Evidence-based complementary and alternative medicine : eCAM 2011 - “...ACJ13070, a lipase from uncultured bacterium; (ACJ13070); XP_390196, a hypothetical protein from Gibberella zeae PH-1; XP_504639, a YALI0E31515p from Yarrowia lipolytica . Triangles and squares represent the residues involved in the formation of the catalytic triad and the oxyanion hole, respectively, and the conserved pentapeptide motifs...”
- Gene cloning and characterization of a novel esterase from activated sludge metagenome
Zhang, Microbial cell factories 2009 - “...amino acid identity to esterase/lipase from Gemmata obscuriglobus UQM 2246 (ZP_02733109) and Yarrowia lipolytica CLIB122 (XP_504639), respectively; and several conserved regions were identified, including the putative active site, HSMGG, a catalytic triad (Ser92, His125 and Asp216) and a LHYFRG conserved motif. The EstAS was overexpressed, purified...”
- “...Gemmata obscuriglobus UQM 2246 (ZP_02733109, 33% identity), followed by the lipase from Yarrowia lipolytica CLIB122 (XP_504639, 31% identity), the putative lipase/esterase from Magnaporthe grisea 70-15 and Saccharomyces cerevisiase Tg12p (XP_368471, 31% identity; and NP_010343, 29% identity, respectively), members of the family of fungal hydrolases. And also,...”
LEPBI_I0777 putative triglyceride lipase; putative signal peptide from Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)'
33% identity, 53% coverage
- Transcriptome Sequencing Reveals Wide Expression Reprogramming of Basal and Unknown Genes in Leptospira biflexa Biofilms
Iraola, mSphere 2016 - “...0.46 9e6 LEPBI_I0104 acdA1 Acyl-CoA dehydrogenase Down 0.33 1e3 LEPBI_I0052 Enoyl-CoA hydratase Down 0.36 4e4 LEPBI_I0777 Putative triglyceride lipase Up 0.69 1.5e6 LEPBI_II0198 fabG 3-Oxoacyl-ACP reductase Down 0.45 7e7 LEPBI_II0199 fabG 3-Oxoacyl-ACP reductase Down 0.39 4e3 LEPBI_II0211 fabG 3-Oxoacyl-ACP reductase Down 0.38 2e3 Iron metabolism LEPBI_I1883...”
- “...used for matrix composition in L.biflexa . This hypothesis is reinforced by the overexpression of LEPBI_I0777 , which codes for a triglyceride lipase (EC 3.1.1.3), allocated to glycerolipid metabolism and involved in degrading triglycerides to single fatty acids ( Table2 ). Furthermore, most enzymes belonging to...”
WP_015677081 lactonizing lipase from Leptospira yanagawae serovar Saopaulo str. Sao Paulo = ATCC 700523
31% identity, 44% coverage
- AMWEst, a new thermostable and detergent-tolerant esterase retrieved from the Albian aquifer
Adjeroud, Applied microbiology and biotechnology 2024 - “...that AMWEst showed moderate similarity (50%) to several lipolytic enzymes including the lactonizing lipase (GenBank: WP_015677081) from Leptospira yanagawae (identity 49%), the triacylglycerol lipase (GenBank: WP_020775775) from Leptospira meyeri (identity 49%), the lactonizing lipase (GenBank: EYF06121) from Chondromyces apiculatus DSM 436 (identity 49%), and the triacylglycerol...”
MGL_4063 uncharacterized protein from Malassezia globosa CBS 7966
27% identity, 52% coverage
- Skin Commensal Fungus Malassezia and Its Lipases
Park, Journal of microbiology and biotechnology 2021 - “...region (PF04083) MGL_1975 [ 11 ] MGL_2531 [ 11 ] Putative serine esterase, Lipase-like (PF05057) MGL_4063 [ 11 ] GDSL-like Lipase/Acylhydrolase (PF00657) MGL_1366 O [ 11 ] M. sympodialis ATCC 42132 Class 3 (PF01764) MSYG_1326 O [ 81 ] MSYG_2002 [ 81 ] MSYG_2462 O [...”
WP_020775775 triacylglycerol lipase from Leptospira meyeri serovar Semaranga str. Veldrot Semarang 173
33% identity, 44% coverage
- AMWEst, a new thermostable and detergent-tolerant esterase retrieved from the Albian aquifer
Adjeroud, Applied microbiology and biotechnology 2024 - “...including the lactonizing lipase (GenBank: WP_015677081) from Leptospira yanagawae (identity 49%), the triacylglycerol lipase (GenBank: WP_020775775) from Leptospira meyeri (identity 49%), the lactonizing lipase (GenBank: EYF06121) from Chondromyces apiculatus DSM 436 (identity 49%), and the triacylglycerol esterase/lipase EstA (alpha/beta hydrolase family) (GenBank: MBB3048100) from Litorivivens lipolytica...”
MGG_00773 triacylglycerol lipase from Pyricularia oryzae 70-15
37% identity, 34% coverage
- Dysfunctional Pro1 leads to female sterility in rice blast fungi
Uchida, iScience 2023 - “...examine whether the DNA sequences were consistent with the phenotype. SNPs within MGG_00779, MGG_00693, and MGG_00773 genes were selected as targets for genotyping because these genes were close to the FS1L, FS1C, and FS1R loci, respectively ( Figures2 A and 2B). Among 37 F 4 progenies,...”
PADG_04319 V-type ATPase, G subunit from Paracoccidioides brasiliensis Pb18
35% identity, 24% coverage
- Proteomic Analysis of Paracoccidioides brasiliensis During Infection of Alveolar Macrophages Primed or Not by Interferon-Gamma
Chaves, Frontiers in microbiology 2019 - “...flavoprotein beta-subunit; PADG_11981, V-type proton ATPase catalytic subunit A; PADG_03175, V-type proton ATPase subunit F; PADG_04319, V-type ATPase, G subunit; PADG_00688, F-type H+-transporting ATPase subunit H; PADG_08391, plasma membrane ATPase; PADG_08394, cytochrome bc1 complex subunit 2; PADG_07042, ATP synthase F1, delta subunit; PADG_04729, ATP synthase subunit...”
- “...flavoprotein subunit alpha; PADG_11468, electron transfer flavoprotein beta-subunit; PADG_11981, V-type proton ATPase catalytic subunit A; PADG_04319, V-type ATPase, G subunit; PADG_03175, V-type proton ATPase subunit F; PADG_00688, F-type H+-transporting ATPase subunit H; PADG_08391, plasma membrane ATPase; PADG_08394, cytochrome bc1 complex subunit 2; PADG_06221, formate dehydrogenase; PADG_01551,...”
MRET_3765 triacylglycerol lipase from Malassezia restricta
35% identity, 22% coverage
- Skin Commensal Fungus Malassezia and Its Lipases
Park, Journal of microbiology and biotechnology 2021 - “...12 , 71 ] MRET_4144 [ 12 , 71 ] Putative serine esterase, Lipase-like (PF05057) MRET_3765 [ 12 , 71 ] GDSL-like Lipase/Acylhydrolase (PF00657) MRET_3994 O [ 12 , 71 ] M. globosa CBS 7966 Class 3 (PF01764) MGL_0279 [ 11 ] MGL_0797 ( MgLIP1 )...”
C9Z1F6 Putative secreted lipase from Streptomyces scabiei (strain 87.22)
41% identity, 27% coverage
- Physical, Chemical and Proteomic Evidence of Potato Suberin Degradation by the Plant Pathogenic Bacterium Streptomyces scabiei
Beaulieu, Microbes and environments 2016 - “...). The abundance of most lipases and esterases detected in this study (Sub1, C9Z6Y6, C9YUN4, C9Z1F6, and C9YTK3) increased over time. The maximal production of these lipolytic enzymes in the late phase of the culture may reflect the bacterial growth phase. In some streptomycetes, esterases and...”
- “...Day 30 Day 60 C9Z6Y6 Cholesterol esterase 0.54 (39.42) 0.68 (34.17) 1.20 (34.19) 1.25 (33.42) C9Z1F6 Lipase 0.22 (16.06) 0.36 (18.09) 0.56 (15.95) 0.62 (16.58) C9YTK3 Lipase 0.11 (8.03) 0.28 (14.01) 0.44 (12.54) 0.36 (9.63) C9YUN4 Cholesterol esterase 0.03 (2.19) 0.10 (5.03) 0.14 (3.99) 0.15 (4.10)...”
NP_001001804 epoxide hydrolase 4 isoform 1 from Mus musculus
Q6IE26 Epoxide hydrolase 4 from Mus musculus
25% identity, 41% coverage
SCO1735 secreted lipase from Streptomyces coelicolor A3(2)
40% identity, 27% coverage
CH_000489 triacylglycerol lipase; EC 3.1.1.3 from Burkholderia cepacia (see paper)
58% identity, 15% coverage
cg0109 triacylglycerol lipase precursor from Corynebacterium glutamicum ATCC 13032
NCgl0079 triacylglycerol lipase from Corynebacterium glutamicum ATCC 13032
30% identity, 46% coverage
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics
Zhang, Scientific reports 2018 - “...413.77 917.71 2.0 52 NCgl0647 cg0779 trpS Tryptophanyl-tRNA synthetase 5.11/37826.54 1378.57 0.00 NC 54 NCgl0079 cg0109 Triacylglycerol lipase precursor 5.11/35814.40 341.27 1637.98 NC 55 NCgl2156 cg2456 Zn-ribbon protein 5.19/26131.12 2802.33 0.00 2.7 56 NCgl0317 cg0391 rmlB2 dTDP-glucose 4,6-dehydratase 5.12/33536.53 717.37 2083.02 NC 57 NCgl2359 cg2686 TetR...”
- Metabolic engineering Corynebacterium glutamicum to produce triacylglycerols
Plassmeier, Metabolic engineering 2016 (PubMed)- “...precursor consumption by deleting four cellular lipases (cg0109, cg0110, cg1676 and cg1320) and the diacylglycerol kinase (cg2849), (iii) enhancement of fatty...”
- “...and precursor consumption by deleting four cellular lipases ( cg0109 , cg0110 , cg1676 and cg1320 ) and the diacylglycerol kinase ( cg2849 ), (iii) enhancement...”
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics
Zhang, Scientific reports 2018 - “...9.89/18278.42 413.77 917.71 2.0 52 NCgl0647 cg0779 trpS Tryptophanyl-tRNA synthetase 5.11/37826.54 1378.57 0.00 NC 54 NCgl0079 cg0109 Triacylglycerol lipase precursor 5.11/35814.40 341.27 1637.98 NC 55 NCgl2156 cg2456 Zn-ribbon protein 5.19/26131.12 2802.33 0.00 2.7 56 NCgl0317 cg0391 rmlB2 dTDP-glucose 4,6-dehydratase 5.12/33536.53 717.37 2083.02 NC 57 NCgl2359 cg2686...”
Q8IUS5 Epoxide hydrolase 4 from Homo sapiens
NP_775838 epoxide hydrolase 4 from Homo sapiens
26% identity, 36% coverage
UH47_02215 YSIRK-targeted triacylglycerol lipase from Staphylococcus pseudintermedius
30% identity, 16% coverage
NPW32_RS11105 YSIRK domain-containing triacylglycerol lipase GehC from Staphylococcus epidermidis
28% identity, 18% coverage
- The first genomic characterization of a stable, hemin-dependent small colony variant strain of Staphylococcus epidermidis isolated from a prosthetic-joint infection
Bogut, Frontiers in microbiology 2023 - “...MSCRAMM family adhesin SdrF; Ser-Asp-rich fibrinogen binding protein Other insertions with frameshift and premature stop NPW32_RS11105 gehC +1nt 336/687 YSIRK domain-containing triacylglycerol lipase GehC NPW32_RS08685 czrB +1(TA) in poli(TA) 72/317 Cation efflux family protein NPW32_RS05835 moaA 2 independent +1A in 2 different poly(A) 111/340333/340 GTP 3,8-cyclase;...”
- “...Genes associated with virulence A 1-nt insertion resulting in the frameshift in the gehC gene (NPW32_RS11105) was detected in 49SCV. The gene encodes the YSIRK domain-containing triacylglycerol lipase. The insertion led to a premature codon and the protein truncation (336 aa/687 aa). It was previously reported...”
SP|Q5HKP6 triacylglycerol lipase; EC 3.1.1.3 from Staphylococcus epidermidis RP62A (see paper)
SERP2297 lipase from Staphylococcus epidermidis RP62A
28% identity, 18% coverage
SE0281 triacylglycerol lipase precursor from Staphylococcus epidermidis ATCC 12228
28% identity, 18% coverage
BJL64_14165, BJL65_15445 YSIRK domain-containing triacylglycerol lipase Lip1 from Staphylococcus aureus
P65289 Lipase 1 from Staphylococcus aureus (strain N315)
28% identity, 17% coverage
- Joint Genomic and Proteomic Analysis Identifies Meta-Trait Characteristics of Virulent and Non-virulent Staphylococcus aureus Strains
Bonar, Frontiers in cellular and infection microbiology 2018 - “...out BJL64_01445 BJL65_01440 Lipase2 Lip2 1075 (I) ch22 out BJL64_11645 BJL65_12915 Toxin n/a ch22 out BJL64_14165 BJL65_15445 Lipase1 Lip1 1075 (I) ch22 surf BJL64_02580 BJL65_02570 50S ribosomal protein L7/L12 RL7/12 0222 (J) CH21 surf BJL64_12760 BJL65_14035 Immunoglobulin-binding protein Sbi Sbi n/a CH21 surf BJL64_14035 BJL65_15315 N-acetylmuramoyl-L-alanine...”
- “...BJL64_01445 BJL65_01440 Lipase2 Lip2 1075 (I) ch22 out BJL64_11645 BJL65_12915 Toxin n/a ch22 out BJL64_14165 BJL65_15445 Lipase1 Lip1 1075 (I) ch22 surf BJL64_02580 BJL65_02570 50S ribosomal protein L7/L12 RL7/12 0222 (J) CH21 surf BJL64_12760 BJL65_14035 Immunoglobulin-binding protein Sbi Sbi n/a CH21 surf BJL64_14035 BJL65_15315 N-acetylmuramoyl-L-alanine amidase...”
- Non-Specific Signal Peptidase Processing of Extracellular Proteins in Staphylococcus aureus N315
Misal, Proteomes 2023 - “...P68800 Fibrinogen-binding protein IVEYNDGTFKYQSR Middle EKKPVSINHN 46 47 IVE Extracellular 100 9 4 5 7 P65289 Lipase 1 NPNIVYKTYTGEATHK Middle ATDLNRKTSL 548 549 NPN Extracellular 80 9 9 0 8 Q99RL2 Immunoglobulin-binding protein sbi LKGITEEQR Middle QQKAFYQVLH 60 61 LKG Extracellular 30 54 39 15 9...”
- Identification of N-terminal protein processing sites by chemical labeling mass spectrometry
Misal, Rapid communications in mass spectrometry : RCM 2019 - “...Q AEAASG 2 2 14 A0A0H3JPG2 Uncharacterized protein A NEEQKSSLLENQK E AYANEE 2 2 15 P65289 Lipase 1 A AEKQVNMGNSQEDTVTAQSIGDQQTR E AQAAEK 2 2 16 Q7A6P2 Thermonuclease A SQTDNGVNR E ANASQT 12 12...”
SAV2671 triacylglycerol lipase precursor from Staphylococcus aureus subsp. aureus Mu50
SA2463 triacylglycerol lipase precursor from Staphylococcus aureus subsp. aureus N315
28% identity, 17% coverage
- Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain
Gill, Journal of bacteriology 2005 - “...vSa vSa vSa4 vSa vSa vSa vSa vSa Sa3 SAV2671 lip SAV0320 geh SA0712 lipAd SA2006 SA1173 SA2003 SA2022 SA2419 Island SAV2009 SAV1824 SAV1828 SAV2008 SAV1829...”
- Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates
Herbert, Infection and immunity 2010 - “...SAOUHSC_00300 SAOUHSC_02710 SAOUHSC_02709 SAOUHSC_03006 SA0674 SA2463 SA2463 SA0309 SA2209 SA2208 SA2463 SAUSA300_0776 SAUSA300_1890 SAUSA300_pUSA010004...”
- “...SAUSA300_1382 SAUSA300_2603 SAUSA300_2603 SAUSA300_0533 SAUSA300_2603 SAUSA300_0955 SA2463 SA0905 SA0746 SA1725 SA0309 SA1637 SA2208 SA2463 SAP003 SA0746 SA1725...”
- Staphylococcal major autolysin (Atl) is involved in excretion of cytoplasmic proteins
Pasztor, The Journal of biological chemistry 2010 - “...amidase SA0905 SA2430 SA0309 SA0309 SA1007 SA2356 SA2463 SA0570 SA2437 00994 02971 00300 00300 01121 02887 03006 00617 02979 Fragment Fragment...”
- “...of LytR a 01501 02583 Fragment 30.8 SA1811 SA2356 SA2463 SA0265 SA2093 SA0620 SA0620 SA2097 02240 02887 03006 00248 02571 00671 00671 02576 33.7a 21.5a 72.9a...”
- Genome-scale transcriptional profiling in Staphylococcus aureus : bringing order out of chaos
Nagarajan, FEMS microbiology letters 2009 - “...arcC carbamate kinase SA2426 arcD arginine/ornithine antiporter SA2427 arcB ornithine carbamoyltransferase SA2428 arcA arginine deiminase SA2463 lip triacylglycerol lipase precursor The contribution of the strains towards gene regulation by agr was statistically tested using LR Chi-Squared tests and the null hypothesis that the strains did not...”
- Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus
Donat, Journal of bacteriology 2009 - “...Pathogenic factors SA0091 SA0483 SA0909 SA1007 SA1752 SA1811 SA2209 SA2463 fbaA fbp sbnC sbnF htrA hit plc clpC fmtA hlb hlb hlgB lip Change (fold)a Description...”
- Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands
Baba, Journal of bacteriology 2008 - “...Sa Sa Sa Sa Sa SA0879 SA0901 SA0900 SA0899 SA0309 SA2463 SA0610 SA2003 SA1160 htrA sspA sspB sspC geh lip lipA hysA nuc MW1054 MW0955 Truncated MW1959 MW2342...”
- Transcriptional profiling reveals that daptomycin induces the Staphylococcus aureus cell wall stress stimulon and genes responsive to membrane depolarization
Muthaiyan, Antimicrobial agents and chemotherapy 2008 - “...NA SA1777 NA SA0833 clpP SA0595 NA SA2463 pepA2 ATP-dependent Clp protease, proteolytic subunit ClpP Peptidase, M20/M25/M40 family Glutamyl-aminopeptidase...”
- Global regulatory impact of ClpP protease of Staphylococcus aureus on regulons involved in virulence, oxidative stress response, autolysis, and DNA repair
Michel, Journal of bacteriology 2006 - “...SA0309 SA0746 SA0901 SA1628 SA1629 SA1630 SA1725 SA2430 SA2463 Other SA0144 SA0145 SA0146 SA0147 SA0148 SA0149 SA0150 SA0151 SA0152 SA0153 SA0154 SA0155 SA0156...”
- The Genera Staphylococcus and Macrococcus
, The ProkaryotesVolume 4: Bacteria: Firmicutes, Cyanobacteria 2006 - “...59-nucleotidase SA0022 None Unknown 62 Lipase SA0309 geh Hydrolytic degradation of lipids - Triacylglycerol lipase SA2463 lip Hydrolytic degradation of lipids 56 Similar to lipase LipA SA0610 None Unknown 77 Probable lipase SA2323 Unknown - 1-Phosphatidylinositol phosphodiesterase SA0091 plc Hydrolysis of phosphatidylinositol - Hyaluronate lyase b...”
- More
LIP1_STAA8 / Q2FUU5 Lipase 1; Glycerol ester hydrolase 1; EC 3.1.1.3 from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see 2 papers)
Q8NUI5 Lipase 1 from Staphylococcus aureus (strain MW2)
SAOUHSC_03006 lipase from Staphylococcus aureus subsp. aureus NCTC 8325
SAUSA300_2603 triacylglycerol lipase precursor from Staphylococcus aureus subsp. aureus USA300_FPR3757
NWMN_2569 lipase precursor from Staphylococcus aureus subsp. aureus str. Newman
SACOL2694 lipase from Staphylococcus aureus subsp. aureus COL
USA300HOU_RS14500 YSIRK domain-containing triacylglycerol lipase Lip1 from Staphylococcus aureus subsp. aureus USA300_TCH1516
28% identity, 17% coverage
- catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
- NAD kinase promotes Staphylococcus aureus pathogenesis by supporting production of virulence factors and protective enzymes
Leseigneur, eLife 2022 - “...pSD1 and NADK sgRNA strains. Uniprot Protein Description Log2R * P Q2FWM4 P0C818 Q2FVK2 P0C7Y1 Q2FUU5 Q2FVK1 Q2G1 0 Q2FWN9 Q2FVK3 Q2FWP0 Q2G2R8 AgrA PsmA4 HlgC PsmA1 Lip1 HlgB Hly LukL2 HlgA LukL1 SspP Accessory gene regulator protein Phenol-soluble modulin alpha 4 Gamma-hemolysin component C Phenol-soluble...”
- A Small Membrane Stabilizing Protein Critical to the Pathogenicity of Staphylococcus aureus
Duggan, Infection and immunity 2020 - “...2.0 0.155 Q2G0L5 sdrC Serine-aspartate repeat-containing protein C 2.0 0.001 Q2G0W6 Uncharacterized protein 2.1 0.006 Q2FUU5 lipA Lipase 1 2.1 0.002 Q2FWZ8 ftnA Bacterial nonheme ferritin 2.1 0.001 Q2G294 Acetyl-coenzyme A synthetase 2.1 0.001 Q2FZI6 purH Bifunctional purine biosynthesis protein PurH 2.2 <0.0001 Q2FVS2 Uncharacterized protein...”
- Antibacterial Activity of Juglone against Staphylococcus aureus: From Apparent to Proteomic
Wang, International journal of molecular sciences 2016 - “...0.010 Q2FY22 50S ribosomal protein L33 2 rpmG2 1 1 5.90 0.459 0.003 Stress response Q2FUU5 lipase 1 lipA 2 3 76.60 1.255 0.004 Q2FZS8 chaperone protein clpB clpB 23 24 98.30 1.211 0.000 Q2G222 N-acetylmuramoyl-l-alanine amidase SAOUHSC_02979 11 11 69.20 1.259 0.000 Q2G0P6 ATP:guanido phosphotransferase...”
- Secreted Toxins From Staphylococcus aureus Strains Isolated From Keratinocyte Skin Cancers Mediate Pro-tumorigenic Inflammatory Responses in the Skin
Krueger, Frontiers in microbiology 2021 - “...sarA Transcriptional regulator SarA Q7A732 0.76 0.00032 hla Alpha-hemolysin Q2G1X0 0.73 0.00066 lip1 Lipase 1 Q8NUI5 0.70 0.0018 sucD Succinate-CoA ligase subunit alpha Q8NX01 0.66 0.0046 SAV1420 Probable CtpA-like serine protease Q99U67 0.66 0.0046 NWMN_1872 65 kDa membrane protein A6QIG2 0.64 0.0053 atl Bifunctional autolysin Q6GAG0...”
- Antibacterial activity and mechanism of flavonoids from Chimonanthus salicifolius S. Y. Hu. and its transcriptome analysis against Staphylococcus aureus
Zhou, Frontiers in microbiology 2022 - “...the RNA-Seq accuracy. As we can observe in Figure 6 , the expression levels of SAOUHSC_03006, SAOUHSC_01879, transcript-32294, and transcript-32305 were downregulated, while the expression levels of transcript-38644, transcript-38651, and transcript-39137 were upregulated. Therefore, the results of the RT-qPCR assay were consisted with the transcriptome analysis,...”
- Staphylococcal saoABC Operon Codes for a DNA-Binding Protein SaoC Implicated in the Response to Nutrient Deficit
Bukowski, International journal of molecular sciences 2022 - “...Replication, recombination and repair (COG0389) srn_3270_sRNA259 2.13 3.87 10 2 Non-coding RNA srn_3270_sRNA259 Non-coding RNA SAOUHSC_03006 gehA 2.22 1.18 10 2 Triacylglycerol lipase precursor Lipid transport and metabolism (COG1075) and Virulence factors (CVF091) SAOUHSC_00912 clpB 2.26 6.14 10 27 ATP-dependent Clp protease ATP-binding, subunit ClpB Posttranslational...”
- Inactivation of farR Causes High Rhodomyrtone Resistance and Increased Pathogenicity in Staphylococcus aureus
Nguyen, Frontiers in microbiology 2019 - “...Alpha-hemolysin ( hla ) 0.7 97.5 SAOUHSC_01135 Phenol-soluble modulin beta1 ( psm 1) 231.1 1113.5 SAOUHSC_03006 Lipase (SAL1) ( gehA ) 0.33 127.7 SAOUHSC_00987 Cysteine protease, staphopain B ( sspB ) 1.44 7.30 SAOUHSC_02866 FarE, fatty acid efflux pump ( farE ) 19.46 92.82 SAOUHSC_02874 Cation...”
- A broad range quorum sensing inhibitor working through sRNA inhibition
Jakobsen, Scientific reports 2017 - “...(encoding , and -hemolysin), splA-F and sspABC (encoding the secreted serine- and cysteine proteases) and SAOUHSC_03006 and SAOUHSC_00300 (encoding lipases) were all down regulated more than 5-fold compared to the untreated cultures. The genes spa and sarH1 , encoding protein A and Staphylococcal accessory regulator-like protein...”
- Type I signal peptidase and protein secretion in Staphylococcus aureus
Schallenberger, Journal of bacteriology 2012 - “...two lipases (Lip1 and Lip2, SAOUHSC_00300 and SAOUHSC_03006, respectively), the autolysins Sle and Atl (SAOUHSC_00427 and SAOUHSC_00994, respectively), an...”
- Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates
Herbert, Infection and immunity 2010 - “...T1 T2 U7 U8 U9 U10 SAOUHSC_03006 SAOUHSC_00994 SAOUHSC_00728 SAOUHSC_03006 SAOUHSC_03006 SAOUHSC_00300 SAOUHSC_02710 SAOUHSC_02709 SAOUHSC_03006 SA0674 SA2463...”
- “...SA0309 SA0107 SA0674 SA0820 SA0129 SA0905 SAOUHSC_00994 SAOUHSC_03006 SAOUHSC_00897 SAOUHSC_02972 SA2463 SA0820 SA2431 SA2463 SA0309 SA2463 SA0107 SA2463 SA0107...”
- Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus
Sibbald, Journal of bacteriology 2010 - “...Extracellular 76.7/7.7 76.7/7.7 76.7/7.7 37.1/8.6 SAOUHSC_03006 SAOUHSC_03006 SAOUHSC_03006 SAOUHSC_00051 88196625 88196625 88196625 88193871 Extracellular...”
- The Phospholipase A1 Activity of Glycerol Ester Hydrolase (Geh) Is Responsible for Extracellular 2-12(S)-Methyltetradecanoyl-Lysophosphatidylglycerol Production in Staphylococcus aureus
Subramanian, mSphere 2023 - “...and isoleucine labeling. Strains AH1263, PDJ171 ( geh ) ( 20 ), JE2, NE338 ( SAUSA300_2603 ::N), NE1175 ( SAUSA300_0320 ::N) or NE104 ( SAUSA300_0641 ::N) were grown in rich broth to a A 600 of 4.0. LPG was extracted from 5mL of cells or 1mL...”
- Staphylococcus aureus Alpha-Toxin Limits Type 1 While Fostering Type 3 Immune Responses
Bonifacius, Frontiers in immunology 2020 - “...aureus USA300: SAUSA300_1058), Lipase (Lip, aka Sal1, pan-genome identifier: SAUPAN006420000, annotation in S. aureus USA300: SAUSA300_2603 - lip) and Phospholipase C (Plc; pan genome identifier: SAUPAN000858000; annotation in S. aureus USA300: SAUSA300_99 - plc) ( 26 ) were produced and purified as described before ( 24...”
- The ω Subunit Governs RNA Polymerase Stability and Transcriptional Specificity in Staphylococcus aureus
Weiss, Journal of bacteriology 2017 - “...Student's t test (**, P 0.01). SAUSA300_0320 and SAUSA300_2603), with molecular masses of 76 and 77 kDa, respectively. Interestingly, a recent report on the...”
- In vivo gene expression in a Staphylococcus aureus prosthetic joint infection characterized by RNA sequencing and metabolomics: a pilot study
Xu, BMC microbiology 2016 - “...L 3 5 2 1.13 Exoenzymes SAU060112_20156 SAUSA300_0224 coa Staphylocoagulase 12 55 7 1.5 SAU060112_40510 SAUSA300_2603 lip Lipase 1 4 95 32 0.51 Adhesins SAU060112_110015 SAUSA300_1917 map MHC analogous protein 458 2894 10 No data SAU060112_10448 SAUSA300_0774 emp Extracellular matrix protein-binding protein emp 77 50 1...”
- Role of lipase from community-associated methicillin-resistant Staphylococcus aureus strain USA300 in hydrolyzing triglycerides into growth-inhibitory free fatty acids
Cadieux, Journal of bacteriology 2014 - “...has two lipase genes, which in USA300 are SAUSA300_2603 (gehA, encoding SAL1) and SAUSA300_0320 (gehB, encoding SAL2). Of the products, SAL1 has been best...”
- “...although the mature SAL2 (SAUSA300_0320) and SAL1 (SAUSA300_2603) lipases share 57% amino acid identity, they have significant differences in substrate...”
- Extracellular proteases are key mediators of Staphylococcus aureus virulence via the global modulation of virulence-determinant stability
Kolar, MicrobiologyOpen 2013 - “...found to be more abundant in the protease-null strain, including two Lipases (Geh = 3.8-fold, SAUSA300_2603 = 2.5-fold), components of the -hemolysin (HlgA = 2.4-fold, HlgC = 2.1-fold), -toxin (twofold), leukotoxin LukE (twofold), and enterotoxin Q (1.5-fold). Our analysis also revealed increased abundance of certain proteins...”
- Role of the accessory gene regulator agr in community-associated methicillin-resistant Staphylococcus aureus pathogenesis
Cheung, Infection and immunity 2011 - “...no. SAUSA300_1922 SAUSA300_2440 SAUSA300_2441 SAUSA300_2603 Resistance SAUSA300_0032 SAUSA300_0033 SAUSA300_0928 Metabolism SAUSA300_0061 SAUSA300_0062...”
- Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates
Herbert, Infection and immunity 2010 - “...SAUSA300_1890 SAUSA300_0320 SAUSA300_1381 SAUSA300_1382 SAUSA300_2603 SAUSA300_2603 SAUSA300_0533 SAUSA300_2603 SAUSA300_0955 SA2463 SA0905 SA0746 SA1725...”
- “...SA1725 U4 U5 U6 U3 SA2463 SA0506 SA2463 SA0905 SAUSA300_2603 SAUSA300_0113 SAUSA300_0955 lip lip geh hlgB hlgC lip lip atl nuc stp nuc stp geh lukF-PV lukS-PV...”
- Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP)
Lentz, Nature chemical biology 2018 - “...mutations in each NWMN_0169, NWMN_0262, NWMN_0748, NWMN_1210, NWMN_1683, NWMN_2092, NWMN_2350, NWMN_2379, NWMN_2434, NWMN_2480, NWMN_2528, and NWMN_2569. Homologs of the aforementioned genes were first identified through the Basic Local Alignment Search Tool (BLASTn) in S. aureus USA300 FPR3757 (GeneBank ID CP000255.1), and corresponding transposon mutants were identified...”
- The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus
Schulthess, Journal of bacteriology 2011 - “...nwmn_1560 nwmn_1702 nwmn_1708 nwmn_1719-1718 nwmn_1719-1718 nwmn_2061 nwmn_2367-2365 nwmn_2553 nwmn_2569 iii iii iii (ii) iii i ii iii ii 7, 36, 46 7, 36, 46...”
- Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates
Herbert, Infection and immunity 2010 - “...N4 NWMN_1069 NWMN_1069 NWMN_0166 NWMN_0166 NWMN_2317 NWMN_1069 NWMN_2569 NWMN_0166 NWMN_2569 NWMN_1872 NWMN_0922 SA2463 SA0222 SA2463 SA1751 SA0905 N3 NWMN_0922...”
- Staphylococcus aureus fur regulates the expression of virulence factors that contribute to the pathogenesis of pneumonia
Torres, Infection and immunity 2010 - “...hydroperoxide reductase subunit C (AphC) Hypothetical protein FLIPr NWMN_2569 NWMN_2543 76.7 77.0 69 6.0 NWMN_0166 NWMN_0962 71.6 49.5 NWMN_1417 No. of matched...”
- Transcriptomic and Metabolomic Analysis of a Fusidic Acid-Selected fusA Mutant of Staphylococcus aureus
Gupta, Antibiotics (Basel, Switzerland) 2022 - “...hld delta-hemolysin precursor 12.60 Fatty acid and phospholipid metabolism SACOL0390 NA lipase-2 precursor, interruption-C 11.32 SACOL2694 gehA triacylglycerol extracellular lipase-1 precursor 19.27 SACOL0212 NA putative 3-hydroxyacyl-CoA dehydrogenase 11.99 SACOL0214 NA putative long-chain-fatty-acid-acetyl-CoA ligase 10.51 Regulatory functions SACOL2026 agrA accessory gene regulator A 20.27 SACOL2023 agrB accessory...”
- Analysis of Genome Sequences of Coagulase-Negative Staphylococci Isolates from South Africa and Nigeria Highlighted Environmentally Driven Heterogeneity
Maguvu, Journal of genomics 2021 - “...+ SACOL1522 Enzymes Lipase ( lip ) + + + + - - - + SACOL2694 Serine V8 protease ( sspA ) - + + + + + + + SACOL1057 Thermonuclease ( nuc ) + + + + + - + + Immune invasion SACOL0860...”
- Identification of commonly expressed exoproteins and proteolytic cleavage events by proteomic mining of clinically relevant UK isolates of Staphylococcus aureus
Smith, Microbial genomics 2016 - “...antigen A E SACOL2660 Immunodominant antigen B E SACOL2666 N -Acetylmuramoyl- l -alanine amidase E SACOL2694 Lipase E SACOL1377 Transketolase U SACOL0962 Glycerophosphoryl diester phosphodiesterase GlpQ, putative U SACOL0303 5-Nucleotidase U SACOL1704 Rod shape-determining protein MreC U SACOL2418 IgG-binding protein SBI U Evidence of post-translational modifications...”
- In vitro and in vivo models of Staphylococcus aureus endophthalmitis implicate specific nutrients in ocular infection
Sadaka, PloS one 2014 - “...nt region complementaryto the 5 end of clfB (clumping factor B) 30.4 (1.2) 6.4 (2.0) SACOL2694 geh Lipase 19.4 (1.4) 3.7 (1.3) 4.4 (1.5) SA1817 f sec3 Enterotoxin type C3 10.9 (3.0) 6.9 (2.4) SA1819 f tst Toxic shock syndrome toxin-1 15.2 (5.5) 8.3 (5.7) Probable...”
- Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates
Herbert, Infection and immunity 2010 - “...SA2463 SA0674 SA0820 COL C1 C2 C3 SACOL2694 SACOL0778 SACOL0962 SAOUHSC_00300 SAOUHSC_00069 SAOUHSC_00728 SAOUHSC_00897 SAOUHSC_00094 glpQ geh glpQ geh spa atl...”
- Comprehensive characterization of methicillin-resistant Staphylococcus aureus subsp. aureus COL secretome by two-dimensional liquid chromatography and mass spectrometry
Ravipaty, Molecular & cellular proteomics : MCP 2010 - “...SACOL2419 SACOL2421 SACOL2422 SACOL2557 SACOL2584 SACOL2660 SACOL2694 Signal peptide with SPase I (3 to 1) a MKALLLKTSVWLVLLFSVMGLWQVSNA...”
- Influence of the two-component system SaeRS on global gene expression in two different Staphylococcus aureus strains
Rogasch, Journal of bacteriology 2006 - “...SACOL2419 SACOL2422 SACOL2421 SACOL1173 SACOL2584 SACOL2694 SACOL1881 SACOL2004 SACOL2006 SACOL0263 SACOL0860 SACOL1490 SACOL1609 SACOL1105 57286833 57284878...”
- Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA
Xu, Frontiers in microbiology 2016 - “...Acetolactate synthase large subunit USA300HOU_RS02055 xprT 2.66 1.47E-03 Xanthine phosphoribosyltransferase USA300HOU_RS12265 2.57 2.28E-03 Hypothetical protein USA300HOU_RS14500 lip 2.16 2.42E-03 Triacylglycerol lipase USA300HOU_RS02060 pbuX 2.16 3.25E-03 NCS2 family nucleobase:cation symporter-2 USA300HOU_RS05895 2.14 1.26E-02 Antibacterial protein USA300HOU_RS04630 dltC 2.11 7.32E-03 D-alaninepoly(phosphoribitol) ligase USA300HOU_RS08560 2.07 1.27E-02 Acetyl-CoA carboxylase biotin...”
MW2590 triacylglycerol lipase precursor from Staphylococcus aureus subsp. aureus MW2
28% identity, 17% coverage
SAR2753 lipase precursor from Staphylococcus aureus subsp. aureus MRSA252
28% identity, 17% coverage
Q6GDD3 Lipase 1 from Staphylococcus aureus (strain MRSA252)
28% identity, 17% coverage
Q6GJZ6 Lipase 2 from Staphylococcus aureus (strain MRSA252)
SAR0317 lipase precursor from Staphylococcus aureus subsp. aureus MRSA252
SAR_RS01585 YSIRK domain-containing triacylglycerol lipase Lip2/Geh from Staphylococcus aureus subsp. aureus MRSA252
29% identity, 16% coverage
- Relative quantitative comparisons of the extracellular protein profiles of Staphylococcus aureus UAMS-1 and its sarA, agr, and sarA agr regulatory mutants using one-dimensional polyacrylamide gel electrophoresis and nanocapillary liquid chromatography coupled with tandem mass spectrometry
Jones, Journal of bacteriology 2008 - “...1 (geh [lip1]; Q6GDD3) and 2 (geh [lip2]; Q6GJZ6), the putative leukocidin F (lukF; Q6GF50) and S (lukF; Q6GF49) subunits, and staphylocoagulase (coa; Q6GK85)....”
- Staphylococcus aureus temperate bacteriophage: carriage and horizontal gene transfer is lineage associated
McCarthy, Frontiers in cellular and infection microbiology 2012 - “...chips, sak, scn, sea, seg, sek, sep 4 SAS0891 SAR0991 5 NWMN_1814 SAR1967 6 SACOL0318 SAR0317 7 NWMN_0992 SAR1102 sak 8 SAB1760 SAR1992 Staphylococcus aureus genomes Sequence data is currently available for the genomes of 74 Staphylococcus aureus isolates on the GenBank database 1 . In...”
- Relative quantitative comparisons of the extracellular protein profiles of Staphylococcus aureus UAMS-1 and its sarA, agr, and sarA agr regulatory mutants using one-dimensional polyacrylamide gel electrophoresis and nanocapillary liquid chromatography coupled with tandem mass spectrometry
Jones, Journal of bacteriology 2008 - “...1 (geh [lip1]; SAR2753) and 2 (geh [lip2]; SAR0317); the putative leukocidin F (luk; SAR2107) and S (luk; SAR2108) subunits; and staphylokinase (sak; SAR2039)...”
- Molecular Characterization of Chimeric Staphylococcus aureus Strains from Waterfowl
Monecke, Microorganisms 2024 - “...Duck Isolate V315 Duck Isolate V482 Goat Isolate 17CS1042 Comments Between lip2 and A5IPQ2 SAUR0317 (SAR_RS01585), SAUR0318 (SAR_RS01590) Positive (ca. 47,400 nt) Positive (ca. 41,500 nt) Negative Negative Negative Negative - Between sufB and MW0800 SAUR0895 (SAR_RS04475), SAUR0896 (SAR_RS04480): Negative Positive (ca. 46,600 nt) Negative Negative...”
CKU_2426 YSIRK domain-containing triacylglycerol lipase Lip1 from Staphylococcus aureus
28% identity, 17% coverage
WP_029418313 triacylglycerol lipase from Bacillus sonorensis
36% identity, 35% coverage
SAU060112_40510 YSIRK domain-containing triacylglycerol lipase Lip1 from Staphylococcus aureus
28% identity, 17% coverage
8k7pA / A0A0U1MWF9 Staphylococcus aureus lipase -psa complex (see paper)
29% identity, 30% coverage
- Ligands: petroselinic acid; zinc ion; calcium ion (8k7pA)
CNAG_07639 triacylglycerol lipase from Cryptococcus neoformans var. grubii H99
33% identity, 19% coverage
- Brain inositol is a novel stimulator for promoting Cryptococcus penetration of the blood-brain barrier
Liu, PLoS pathogens 2013 - “...CNL04840 CNAG_05138 Exo-beta-1,3-glucanase 2.54 CNC02410 CNAG_01737 Methyl sterol oxidase 2.54 CNB00990 CNAG_03596 2-Oxoglutarate_dehydrogenase_complex 2.53 CNE02710 CNAG_07639 Triacylglycerol lipase 2.50 Signal transduction CNA01180 CNAG_00130 Serine/threonine protein kinase 5.11 CNB05690 CNAG_04090 bZip transcription factor 3.13 CNB01230 CNAG_03621 Cyclophilin A 2.96 CNH00140 CNAG_05348 Small_GTPase_CDC42 2.88 CNH00970 CNAG_05431 Transcription factor...”
LIP2_STAA8 / Q2G155 Lipase 2; Glycerol ester hydrolase 2; EC 3.1.1.3 from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see paper)
SP|Q5HJ48 triacylglycerol lipase; EC 3.1.1.3 from Staphylococcus aureus subsp. aureus COL (see paper)
SAOUHSC_00300 lipase precursor from Staphylococcus aureus subsp. aureus NCTC 8325
28% identity, 17% coverage
- catalytic activity: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) (RHEA:12044)
- Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains
Jin, Frontiers in microbiology 2020 - “...3.2598 3.29 0.0003595 0.00042238 Serine protease SplB SAOUHSC_01939 3.7964 1.23 2.73E-08 4.78E-08 Serine protease SplC SAOUHSC_00300 2.4787 1.03 2.09E-39 1.04E-38 Lipase SAOUHSC_02706 2.0555 6.25 2.26E-10 4.59E-10 Immunoglobulin G-binding protein Sbi SAOUHSC_00177 6.0315 ND 0 0 Maltose ABC transporter permease Hypothetical protein SAOUHSC_00179 6.4487 ND 0 0...”
- A broad range quorum sensing inhibitor working through sRNA inhibition
Jakobsen, Scientific reports 2017 - “...and -hemolysin), splA-F and sspABC (encoding the secreted serine- and cysteine proteases) and SAOUHSC_03006 and SAOUHSC_00300 (encoding lipases) were all down regulated more than 5-fold compared to the untreated cultures. The genes spa and sarH1 , encoding protein A and Staphylococcal accessory regulator-like protein are both...”
- Type I signal peptidase and protein secretion in Staphylococcus aureus
Schallenberger, Journal of bacteriology 2012 - “...arylomycin, including two lipases (Lip1 and Lip2, SAOUHSC_00300 and SAOUHSC_03006, respectively), the autolysins Sle and Atl (SAOUHSC_00427 and SAOUHSC_00994,...”
- The Staphylococcus aureus KdpDE two-component system couples extracellular K+ sensing and Agr signaling to infection programming
Xue, Infection and immunity 2011 - “...SAOUHSC_00123 SAOUHSC_00124 SAOUHSC_00125 SAOUHSC_00126 SAOUHSC_00127 SAOUHSC_00300 SAOUHSC_02260 SAOUHSC_02709 SAOUHSC_02710 Gene product 2162 XUE ET AL. FIG....”
- Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways
Falord, PloS one 2011 - “...haemolytic protein 98.4 <1.0E16 SAOUHSC_02260 hld Delta-haemolysin 80.1 <1.0E16 SAOUHSC_01110 Putative fibrinogen-binding protein 8.5 <1.0E16 SAOUHSC_00300 geh Lipase precursor 4.7 <1.0E16 SAOUHSC_01953 epiA Lantibiotic epidermin precursor EpiA 3.2 <1.0E16 SAOUHSC_02163 hlb Beta-haemolysin 2.8 5.10E11 SAOUHSC_02167 HP Similar to complement inhibitor protein SCIN 2.8 <1.0E16 SAOUHSC_01121 hla...”
- Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates
Herbert, Infection and immunity 2010 - “...SAOUHSC_03006 SAOUHSC_00994 SAOUHSC_00728 SAOUHSC_03006 SAOUHSC_03006 SAOUHSC_00300 SAOUHSC_02710 SAOUHSC_02709 SAOUHSC_03006 SA0674 SA2463 SA2463 SA0309 SA2209...”
- “...SA0820 COL C1 C2 C3 SACOL2694 SACOL0778 SACOL0962 SAOUHSC_00300 SAOUHSC_00069 SAOUHSC_00728 SAOUHSC_00897 SAOUHSC_00094 glpQ geh glpQ geh spa atl lip geh lip...”
- Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus
Sibbald, Journal of bacteriology 2010 - “...35.9/9.1 35.9/9.1 36.7/9.8 35.7/9.7 SAOUHSC_00300 SAOUHSC_02240 SAOUHSC_02240 SAOUHSC_01121 SAOUHSC_01121 SAOUHSC_01121 SAOUHSC_01121 SAOUHSC_02710...”
SA0309 glycerol ester hydrolase from Staphylococcus aureus subsp. aureus N315
Q7A7P2 Lipase 2 from Staphylococcus aureus (strain N315)
SAV0320 glycerol ester hydrolase from Staphylococcus aureus subsp. aureus Mu50
BJL64_01445, BJL65_01440 YSIRK domain-containing triacylglycerol lipase Lip2/Geh from Staphylococcus aureus
28% identity, 16% coverage
- VraSR and Virulence Trait Modulation during Daptomycin Resistance in Methicillin-Resistant <i>Staphylococcus aureus</i> Infection
Taglialegna, mSphere 2019 - “...2.458 SA2291 fnbA Fibronectin binding protein A 1.742 SA2290 fnbB Fibronectin binding protein B 2.692 SA0309 geh Lipase 1.274 SA1756 hlb Truncated -hemolysin 3.075 SAS065 hld -Hemolysin 8.235 SA2207 hlgA -Hemolysin component A 1.412 SA2209 hlgB -Hemolysin component B 2.195 SA2208 hlgC -Hemolysin component C 1.476...”
- Novel single-nucleotide variations associated with vancomycin resistance in vancomycin-intermediate Staphylococcus aureus
Lin, Infection and drug resistance 2018 - “...Transcriptome analysis data 21 SA1193 fmtC 13636121366134 Oxacillin resistance-related FmtC protein Transcriptome analysis data 10 SA0309 geh 365458367533 Glycerol ester hydrolase Transcriptome analysis data 16 SA0430 gltB 490664495163 Glutamate synthase large subunit Transcriptome analysis data 16 SA0614 graR 708245708919 Hypothetical protein, similar to two-component response regulator...”
- The effect of skin fatty acids on Staphylococcus aureus
Neumann, Archives of microbiology 2015 - “...decarboxylas 1.12 280 SA0265 LytM Peptidoglycan hydrolase 0.15 454 SA0265 LytM Peptidoglycan hydrolase 0.13 460 SA0309 Geh Glycerol ester hydrolase 2.29 170 SA0309 Geh Glycerol ester hydrolase 3.08 171 SA0309 Geh Glycerol ester hydrolase 2.84 172 SA0309 Geh Glycerol ester hydrolase 1.81 175 SA0309 Geh Glycerol...”
- “...177 SA0309 Geh Glycerol ester hydrolase 9.64 199 SA0309 Geh Glycerol ester hydrolase 3.28 212 SA0309 Geh Glycerol ester hydrolase 1.76 224 SA0309 Geh Glycerol ester hydrolase 21.07 229 SA0309 Geh Glycerol ester hydrolase 5.23 272 SA0309 Geh Glycerol ester hydrolase 1.81 282 SA0309 Geh Glycerol...”
- The C-terminal region of the RNA helicase CshA is required for the interaction with the degradosome and turnover of bulk RNA in the opportunistic pathogen Staphylococcus aureus
Giraud, RNA biology 2015 - “...protein SA0285 2.4 2.1 1.1 3.1 SA0301 SA0300-SA0302 Pseudouridine-5-phosphate glycosidase psuG 2.1 1.8 3.1 3.2 SA0309 Lipase 2 geh 2.0 2.7 2.3 8.3 SA0318 SA0318-SA0321 Uncharacterized protein, similar to Ascorbate-specific permease IIC component UlaA ulaA 2.1 2.1 2.0 1.8 SA0319 SA0319-SA0321 Uncharacterized protein SA0319 2.5 2.1...”
- Perturbation of Staphylococcus aureus gene expression by the enoyl-acyl carrier protein reductase inhibitor AFN-1252
Parsons, Antimicrobial agents and chemotherapy 2013 - “...genes downregulated by AFN-1252 Locus tag Gene SA0309 SA0660 SA0661 SA0662 SA0663 SA1000 SA1003 SA1007 SA1750 SA1752 SA1811 SA1812 SA2206 SA2207...”
- Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates
Herbert, Infection and immunity 2010 - “...SAOUHSC_02709 SAOUHSC_03006 SA0674 SA2463 SA2463 SA0309 SA2209 SA2208 SA2463 SAUSA300_0776 SAUSA300_1890 SAUSA300_pUSA010004 SAUSA300_0776 SAUSA300_1890...”
- “...SAUSA300_0533 SAUSA300_2603 SAUSA300_0955 SA2463 SA0905 SA0746 SA1725 SA0309 SA1637 SA2208 SA2463 SAP003 SA0746 SA1725 U4 U5 U6 U3 SA2463 SA0506 SA2463...”
- Staphylococcal major autolysin (Atl) is involved in excretion of cytoplasmic proteins
Pasztor, The Journal of biological chemistry 2010 - “...Hypothetical protein SA2437F N-Acetylmuramoyl-L-alanine amidase SA0905 SA2430 SA0309 SA0309 SA1007 SA2356 SA2463 SA0570 SA2437 00994 02971 00300 00300 01121...”
- “...hla_rev CTAATACGACTCACTATAGGGAGAGGGTTCCAAGAATCTCTATC geh_for (SA0309) CAGTGTTAGCGGCTACAATG geh_rev CTAATACGACTCACTATAGGGAGAGAAGTTGAAGTTGGTGCTGC Staphylococcal...”
- An RpoB mutation confers dual heteroresistance to daptomycin and vancomycin in Staphylococcus aureus
Cui, Antimicrobial agents and chemotherapy 2010 - “...murD div1b opp-2D ebhA Other functions SA0128 SA0222 SA0309 SA0519 SA0520 SA0521 SA0641 SA0723 SA0743 SA0746 SA1145 SA1396 SA1452 SA1549 SA1559 SA1577 SA1617...”
- More
- Non-Specific Signal Peptidase Processing of Extracellular Proteins in Staphylococcus aureus N315
Misal, Proteomes 2023 - “...protein AKIKAIKGNAEQSR Near C-term LSYLDYKKQKPN 199 200 AKI Predicted membrane/Signal 30 18 15 3 30 Q7A7P2 Lipase 2 IINDLLR Near C-term RKGAELANFYTG 670 671 IIN Extracellular 20 9 9 0 31 A0A0H3JMK9 SA1273 protein ALSAGQR Near C-term YQSVENVVENID 222 223 ALS not Known 100 4 4...”
- Transcriptional Analysis of the Effects of Gambogic Acid and Neogambogic Acid on Methicillin-Resistant Staphylococcus aureus
Hua, Frontiers in pharmacology 2019 - “...SAV0285 None type VII secretion protein EsaB 2.56 2.51 SAV0315 nanA N-acetylneuraminate lyase 1.75 2.25 SAV0320 geh lipase 2.81 3.84 SAV0432 hsdS restriction endonuclease subunit S 1.98 2.32 SAV0450 None cobalamin synthesis protein CobW 2.26 2.82 SAV0458 None sodium-dependent transporter 1.31 2.13 SAV0465 None peptidase M23B...”
- Genomic, Transcriptomic and Metabolomic Studies of Two Well-Characterized, Laboratory-Derived Vancomycin-Intermediate Staphylococcus aureus Strains Derived from the Same Parent Strain
Hattangady, Antibiotics (Basel, Switzerland) 2015 - “...of the SarA transcriptional regulator that often exerts effects opposite of agr [ 48 ] SAV0320 geh glycerol ester hydrolase 2.7 12 9.3 rot -regulated lipase translated as an inactive, truncated form due bacteriophage L54a integration [ 10 , 49 ] SAV2637 aur zinc metalloproteinase aureolysin...”
- An antibiotic that inhibits a late step in wall teichoic acid biosynthesis induces the cell wall stress stimulon in Staphylococcus aureus
Campbell, Antimicrobial agents and chemotherapy 2012 - “...subunit A fruB Fructose 1-phosphate kinase SACOL1321 SAV0320 glpD geh Aerobic glycerol-3-phosphate dehydrogenase Glycerol ester hydrolase SA2102 SAV1413 SAS2105...”
- Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures
Even, Applied and environmental microbiology 2009 - “...2.3 Enzyme genes SAV0815 SAV1324 SAV1811 SAV1813 SAV1046 SAV0320 MW0769 MW1211 MW1753 MW1755 MW0930 MW0297 nuc nuc splC splA sspC geh Staphylococcal nuclease...”
- Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain
Gill, Journal of bacteriology 2005 - “...vSa4 vSa vSa vSa vSa vSa Sa3 SAV2671 lip SAV0320 geh SA0712 lipAd SA2006 SA1173 SA2003 SA2022 SA2419 Island SAV2009 SAV1824 SAV1828 SAV2008 SAV1829 SAV1825...”
- Joint Genomic and Proteomic Analysis Identifies Meta-Trait Characteristics of Virulent and Non-virulent Staphylococcus aureus Strains
Bonar, Frontiers in cellular and infection microbiology 2018 - “...(V) ch22 out BJL64_03485 BJL65_03480 Glycerol phosphate lipoteichoic acid synthase LtaS 1368 (M) ch22 out BJL64_01445 BJL65_01440 Lipase2 Lip2 1075 (I) ch22 out BJL64_11645 BJL65_12915 Toxin n/a ch22 out BJL64_14165 BJL65_15445 Lipase1 Lip1 1075 (I) ch22 surf BJL64_02580 BJL65_02570 50S ribosomal protein L7/L12 RL7/12 0222 (J)...”
- “...ch22 out BJL64_03485 BJL65_03480 Glycerol phosphate lipoteichoic acid synthase LtaS 1368 (M) ch22 out BJL64_01445 BJL65_01440 Lipase2 Lip2 1075 (I) ch22 out BJL64_11645 BJL65_12915 Toxin n/a ch22 out BJL64_14165 BJL65_15445 Lipase1 Lip1 1075 (I) ch22 surf BJL64_02580 BJL65_02570 50S ribosomal protein L7/L12 RL7/12 0222 (J) CH21...”
SAUSA300_0320 triacylglycerol lipase precursor from Staphylococcus aureus subsp. aureus USA300_FPR3757
B7H15_01765 YSIRK domain-containing triacylglycerol lipase Lip2/Geh from Staphylococcus aureus
28% identity, 17% coverage
- Staphylococcus aureus lipid factors modulate melanoma cell clustering and invasion
Giese, Disease models & mechanisms 2024 - “...mutants to promote melanoma clustering. A variety of lipases, including Sal2 (also known as GehB; SAUSA300_0320) are secreted by S. aureus . Sal2 has ester hydrolase activity, and cleaves both short and long chain triglycerides ( Cadieux et al., 2014 ). We found that supernatant from...”
- The Phospholipase A1 Activity of Glycerol Ester Hydrolase (Geh) Is Responsible for Extracellular 2-12(S)-Methyltetradecanoyl-Lysophosphatidylglycerol Production in Staphylococcus aureus
Subramanian, mSphere 2023 - “...AH1263, PDJ171 ( geh ) ( 20 ), JE2, NE338 ( SAUSA300_2603 ::N), NE1175 ( SAUSA300_0320 ::N) or NE104 ( SAUSA300_0641 ::N) were grown in rich broth to a A 600 of 4.0. LPG was extracted from 5mL of cells or 1mL of supernatant from 0.2m...”
- Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Genetic Factors Involved in Human Endothelial Cells Damage, an Important Phenotype Correlated with Persistent Endovascular Infection
Xiao, Antibiotics (Basel, Switzerland) 2022 - “...6.02 SAUSA300_1384 hypothetical phiSLT ORF100b-like protein, holin 23.25 6.98 SAUSA300_1950 hypothetical hypothetical protein 23.24 9.64 SAUSA300_0320 gehB triacylglycerol lipase 23.13 9.02 SAUSA300_0370 hypothetical putative enterotoxin 23.06 9.01 SAUSA300_1224 hypothetical conserved hypothetical protein 22.85 4.12 SAUSA300_1925 hypothetical phiPVL ORF17-like protein 22.72 9.85 SAUSA300_1271 hypothetical hydrolase-like protein 22.57...”
- Tools, Strains, and Strategies To Effectively Conduct Anaerobic and Aerobic Transcriptional Reporter Screens and Assays in Staphylococcus aureus
Price, Applied and environmental microbiology 2021 (secret) - The cell envelope of Staphylococcus aureus selectively controls the sorting of virulence factors
Zheng, Nature communications 2021 - “...proteins that were predicted to be secreted through the Sec pathway (SignalP-5.0 51 ): Geh (SAUSA300_0320), ScaH (SAUSA300_2579), Hel (SAUSA300_0307), SsaA (SAUSA300_2249), IsaA (SAUSA300_2506), and IsaB (SAUSA300_2573). We engineered USA300 strains to produce C-terminal His-tag fusions of these proteins, as well as -toxin (Hla) as a...”
- Bacterial lipolysis of immune-activating ligands promotes evasion of innate defenses
Chen, Proceedings of the National Academy of Sciences of the United States of America 2019 - “...in this screen carried a transposon insertion in SAUSA300_0320, the gene encoding Geh. Cell-free supernatant derived from the geh::Tn mutant elicited enhanced...”
- Identification of Extracellular DNA-Binding Proteins in the Biofilm Matrix
Kavanaugh, mBio 2019 - “...19.4 9.7 Spent medium 7 SAUSA300_0955 AtlA, full length Surface, equatorial ring 116.5 9.6 8 SAUSA300_0320 Geh, triacylglycerol lipase Secreted 72.2 9.0 8 SAUSA300_0955 AtlA amidase domain Surface equatorial ring 60.8 9.6 8 SAUSA300_2579 N -Acetylmuramoyl- l - alanine amidase Surface, equatorial ring 69.2 6.4 9...”
- Identification of Staphylococcus aureus Factors Required for Pathogenicity and Growth in Human Blood
Connolly, Infection and immunity 2017 - “...SAUSA300_1305 NE1391 Dihydrolipoamide succinyltransferase (OdhB) Slightly reduced growth Slightly reduced growth Increased hemolysis Increased hemolysis SAUSA300_0320 NE1775 Triacylglycerol lipase (LipA) Slightly reduced growth Increased hemolysis B SAUSA300_1989 NE95 Accessory gene regulator protein B (AgrB) Reduced hemolysis SAUSA300_1991 NE873 Accessory gene regulator protein C (AgrC) Slightly reduced...”
- More
- Staphylococcus aureus Stress Response to Bicarbonate Depletion
Liberini, International journal of molecular sciences 2024 - “...protein 64.6 31 B7H15_14690 Aureolysin, zinc metalloproteinase 44.6 1.2 B7H15_00545 Phospholipase C, phosphatidylinositol 23.1 1.7 B7H15_01765 Lip2(geh), triacylglycerol lipase 20.3 200 Transporters B7H15_01540 Type VII secretion effector EsxA (and all other type VII secretion protein genes) 56.5 102 B7H15_01675 Formate/nitrite transporter family protein 33.7 1.7 B7H15_01120...”
CNE02710 lipase 2 from Cryptococcus neoformans var. neoformans JEC21
33% identity, 20% coverage
- Brain inositol is a novel stimulator for promoting Cryptococcus penetration of the blood-brain barrier
Liu, PLoS pathogens 2013 - “...2.59 CNL04840 CNAG_05138 Exo-beta-1,3-glucanase 2.54 CNC02410 CNAG_01737 Methyl sterol oxidase 2.54 CNB00990 CNAG_03596 2-Oxoglutarate_dehydrogenase_complex 2.53 CNE02710 CNAG_07639 Triacylglycerol lipase 2.50 Signal transduction CNA01180 CNAG_00130 Serine/threonine protein kinase 5.11 CNB05690 CNAG_04090 bZip transcription factor 3.13 CNB01230 CNAG_03621 Cyclophilin A 2.96 CNH00140 CNAG_05348 Small_GTPase_CDC42 2.88 CNH00970 CNAG_05431 Transcription...”
Q8NYC2 Lipase 2 from Staphylococcus aureus (strain MW2)
MW0297 glycerol ester hydrolase from Staphylococcus aureus subsp. aureus MW2
28% identity, 17% coverage
- Secreted Toxins From Staphylococcus aureus Strains Isolated From Keratinocyte Skin Cancers Mediate Pro-tumorigenic Inflammatory Responses in the Skin
Krueger, Frontiers in microbiology 2021 - “...acid synthase Q99VQ4 0.61 0.0094 splA Serine protease SplA Q99T60 0.60 0.011 lip2 Lipase 2 Q8NYC2 0.60 0.011 adk Adenylate kinase Q6GEK4 0.58 0.014 fda Fructose-bisphosphate aldolase class 1 Q5HCU6 0.58 0.014 rpsH 30S ribosomal protein S8 Q6GEJ7 0.58 0.014 SAV1015 Putative phosphoesterase SAV1015 Q99V77 0.58...”
- Staphylococcal Superantigen-Like Protein 1 and 5 (SSL1 & SSL5) Limit Neutrophil Chemotaxis and Migration through MMP-Inhibition
Koymans, International journal of molecular sciences 2016 - “...(PHLC STAAU), HlgA (NWMN_2318), HlgB (HLGB STAAU), HlgC (HLGC STAAU), IsdA (SA0977), IsdC (SA0978), Lipase (MW0297), LukA (NWMN_1928), LukB (NWMN_1927), LukD (SAUSA300_1768), LukE (SAUSA300_1769), LukF-PV (O50604 STAAU), LukS-PV (O50603 STAAU), LukM (Acc No: WP 063651016), ORF-D (MW0205), MW1225, NWMN_0337, NWMN_0401, NWMN_0402, NWMN_2283, PrsA (SAOUHSC_01972), rplQ (SAOUHSC_02484),...”
- Expression of virulence factors by Staphylococcus aureus grown in serum
Oogai, Applied and environmental microbiology 2011 - “...FeCl3 GeneIDa MW1056 MW0759 MW1889 MW2558 MW0932 MW1755 MW0297 MW0206 MW1885 MW2341 MW0084 MW0130 MW2586 MW0516 MW0764 MW2421 MW1884 MW0088 MW0094 MW1014...”
- “...MW1767 MW1379 MW1056 MW0759 MW1889 MW2558 MW0932 MW1755 MW0297 MW0206 MW1885 MW2341 MW0084 MW0130 MW2586 MW0516 MW0764 MW2421 MW1884 MW0088 MW0094 MW1014 a Gene...”
- Rsp inhibits attachment and biofilm formation by repressing fnbA in Staphylococcus aureus MW2
Lei, Journal of bacteriology 2011 - “...change Secreted proteins MW1755 MW1754 MW1753 MW1752 MW1850 MW1767 MW1940 MW0297 MW0345 MW0767 MW1880 6.7 9.0 6.0 6.0 5.4 2.9 2.8 2.0 2.3 2.8 2.5 splA splB splC...”
- The SaeR/S gene regulatory system is essential for innate immune evasion by Staphylococcus aureus
Voyich, The Journal of infectious diseases 2009 - “...antiholin-like protein LrgB 2.57 & 1.92 ME & ES MW0292 nanA N-acetylneuraminate lyase 2.19 ME MW0297 geh glycerol ester hydrolase 4.09 & 3.00 ME & ES MW0394 set26 exotoxin homolog 16.46 ME MW0568 adh1 alcohol dehydrogenase 1.65 & 4.34 ME & ES MW0667 saeS histidine protein...”
- Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures
Even, Applied and environmental microbiology 2009 - “...SAV1046 SAV0320 MW0769 MW1211 MW1753 MW1755 MW0930 MW0297 nuc nuc splC splA sspC geh Staphylococcal nuclease Thermonuclease Serine protease Serine protease...”
- Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands
Baba, Journal of bacteriology 2008 - “...splB splC splF Sa Sa Sa Sa MW0903 MW0932 MW0931 MW0930 MW0297 MW2590 MW0617 MW2129 MW1211 htrA sspA sspB sspC geh lip lipA hysA nuc NM3 NM3 MW1885 sak Sa3mw...”
- Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain
Gill, Journal of bacteriology 2005 - “...hlgC d Island MW0617 lipAd SA2421 hlgC d RP62a MW2590 lip MW0297 geh SA0610 lipAd vSa vSa Locus and gene vSa SE0891 nuc SE1570 SE0829d SE0436 clpP SE1004 nuc SP...”
CKU_0273 YSIRK domain-containing triacylglycerol lipase Lip2/Geh from Staphylococcus aureus
28% identity, 16% coverage
NWMN_0262 truncated triacylglycerol lipase precursor from Staphylococcus aureus subsp. aureus str. Newman
SACOL0317 lipase precursor, interruption-N from Staphylococcus aureus subsp. aureus COL
28% identity, 18% coverage
- Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP)
Lentz, Nature chemical biology 2018 - “...S5 . In brief, transposon mutagenesis was used to generate insertion mutations in each NWMN_0169, NWMN_0262, NWMN_0748, NWMN_1210, NWMN_1683, NWMN_2092, NWMN_2350, NWMN_2379, NWMN_2434, NWMN_2480, NWMN_2528, and NWMN_2569. Homologs of the aforementioned genes were first identified through the Basic Local Alignment Search Tool (BLASTn) in S. aureus...”
- CodY in Staphylococcus aureus: a regulatory link between metabolism and virulence gene expression
Pohl, Journal of bacteriology 2009 - “...NWMN_0103 NWMN_0104 NWMN_0105 NWMN_0107 NWMN_0108 NWMN_0262 NWMN_0525 NWMN_1084 NWMN_1246 NWMN_1872 NWMN_nd NWMN_0166 NWMN_0394 NWMN_2392 NWMN_2399 NWMN_0071...”
- Comprehensive characterization of methicillin-resistant Staphylococcus aureus subsp. aureus COL secretome by two-dimensional liquid chromatography and mass spectrometry
Ravipaty, Molecular & cellular proteomics : MCP 2010 - “...SACOL0263 SACOL0095 SACOL0119 SACOL0270 SACOL0303 SACOL0317 SACOL0442 SACOL0480 SACOL0507 SACOL0610 SACOL0669 SACOL0723 SACOL0755 SACOL0856 SACOL0858 SACOL0859...”
- A defect in menadione biosynthesis induces global changes in gene expression in Staphylococcus aureus
Kohler, Journal of bacteriology 2008 - “...Among them are geh, hlY, and hlb and the SACOL0317 (lip) and SACOL2418 (sbi) genes (see Table S2 in the supplemental material). In accordance to this...”
- Influence of the two-component system SaeRS on global gene expression in two different Staphylococcus aureus strains
Rogasch, Journal of bacteriology 2006 - “...SACOL0593 SACOL0842 SACOL1180 SACOL2117 SACOL1782 SACOL0838 SACOL0317 SACOL0962 57286825 57286570 57284860 57286563 57286673 57284318 57285784 57285609 57284304...”
- “...SACOL0480 SACOL0858 SACOL0479 SACOL0478 SA1755 (N315) SACOL0317 SACOL0442 57284453 57285784 57284321 57285783 57284320 57285507 57284319 57285506 57285505...”
CPI83_19975 triacylglycerol lipase from Rhodococcus sp. H-CA8f
29% identity, 34% coverage
WP_020452056 triacylglycerol lipase from Bacillus paralicheniformis
36% identity, 28% coverage
SE0185 glycerol ester hydrolase from Staphylococcus epidermidis ATCC 12228
26% identity, 18% coverage
NPW32_RS02885 YSIRK domain-containing triacylglycerol lipase GehD from Staphylococcus epidermidis
26% identity, 18% coverage
WP_003183220 triacylglycerol lipase from Bacillus licheniformis
35% identity, 35% coverage
SERP2388 lipase from Staphylococcus epidermidis RP62A
SERP_RS11730 YSIRK domain-containing triacylglycerol lipase GehD from Staphylococcus epidermidis RP62A
26% identity, 18% coverage
cg0110 triacylglycerol lipase precursor from Corynebacterium glutamicum ATCC 13032
36% identity, 27% coverage
H0B8D4 Putative secreted lipase from Streptomyces sp. W007
SPW_1544 triacylglycerol lipase from Streptomyces sp. W007
39% identity, 27% coverage
- The Meaning of Mas
Bader, Hypertension (Dallas, Tex. : 1979) 2018 - “...51 kDA Catalytic subunit of the mitochondrial processing protease Strepto-myces sp. W007 mas1 (SPW_1544) Mas1 H0B8D4 MArine Streptomyces 1 Uniprot: H0B8D4 31 kDa Putative secreted thermostable lipase...”
- The Meaning of Mas
Bader, Hypertension (Dallas, Tex. : 1979) 2018 - “...SGD: S000004153 51 kDA Catalytic subunit of the mitochondrial processing protease Strepto-myces sp. W007 mas1 (SPW_1544) Mas1 H0B8D4 MArine Streptomyces 1 Uniprot: H0B8D4 31 kDa Putative secreted thermostable lipase...”
WP_000517067 triacylglycerol lipase from Bacillus cereus B4264
30% identity, 31% coverage
- Analysis of Comparative Sequence and Genomic Data to Verify Phylogenetic Relationship and Explore a New Subfamily of Bacterial Lipases
Masomian, PloS one 2016 - “...sequences are for the following organisms: WP_006094994, Bacillus pseudomycoides DSM 12442; EEM11082, Bacillus mycoides Rock3-17; WP_000517067, Bacillus cereus B4264; ACP13431, Bacillus anthracis CDC684; AY260764, Geobacillus zalihae strain T1; AY855077, Bacillus sp. L2; AY787835, Bacillus sp. 42; AF134840, Bacillus thermoleovorans ; X95309, Bacillus thermocatenulatus; AF237623, Bacillus stearothermophilus...”
- “...B . pseudomycoides DSM 12442 (WP_006094994), B . mycoides Rock3-17 (EEM11082), B . cereus B4264 (WP_000517067) and B . anthracis str. CDC684 (ACP13431) that were previously identified in bacterial genome sequences with around 6260% identity. HZ lipase is moderately related to sub-families I.5 and I.6 of...”
5h6bA / H0B8D4 Crystal structure of a thermostable lipase from marine streptomyces (see paper)
39% identity, 27% coverage
Pden_4983 alpha/beta hydrolase fold from Paracoccus denitrificans PD1222
36% identity, 32% coverage
- H-NOX Influences Biofilm Formation, Central Metabolism, and Quorum Sensing in <i>Paracoccus denitrificans</i>
Islam, Journal of proteome research 2024 - “...gene whose expression was constitutive at the protein level (A1B5X4, see Table S1 ). Pden_4985, Pden_4983, and Pden_1517 are annotated as the / hydrolase fold enzyme (A/B), pyruvate dehydrogenase E1 subunit (PDH), and XRE family transcriptional regulator (TR), respectively. All are downregulated comparably at both the...”
- “...Further, we see a downregulation in proteins annotated as E1 (Pden_4984 and Pden_4985) and E2 (Pden_4983) components of PDH. This is counterintuitive, given that downregulation of these proteins would presumably lead to an increase of the substrate pyruvate in the hnox strain. However, it is unknown...”
lip / CAB95850.2 lipase precursor from Bacillus licheniformis (see 2 papers)
35% identity, 28% coverage
TTE0555 predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold from Thermoanaerobacter tengcongensis MB4
27% identity, 38% coverage
- Carboxylic ester hydrolases from hyperthermophiles
Levisson, Extremophiles : life under extreme conditions 2009 - “...of 1h at 100C 44.5 (1) Levisson et al. ( 2007 ) Thermoanaerobacter tengcongensis Esterase TTE0555 p NP-C3/C4 NR NR NR 70 9 Half-life of 1.5h at 70C 43 Zhang et al. ( 2003 ) Thermosyntropha lipolytica Lipase (LipA) NR p NP-C12 NR NR NR 96...”
WP_126510627 YSIRK-targeted triacylglycerol lipase from Staphylococcus warneri
26% identity, 18% coverage
WP_043054382 triacylglycerol lipase from Bacillus paralicheniformis
34% identity, 35% coverage
SCO1265 lipase from Streptomyces coelicolor A3(2)
36% identity, 27% coverage
- Machine-Learning Analysis of Streptomyces coelicolor Transcriptomes Reveals a Transcription Regulatory Network Encompassing Biosynthetic Gene Clusters
Lee, Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 - “...). Interestingly, SigJ and SCO3450 seem to regulate catabolic genes clustered at specific genomic loci (SCO1265 SCO1288 and SCO3437 SCO3465, respectively), and their overexpression may promote phosphate consumption, resulting in actinorhodin production. Overall, each sigma factor affects antibiotic production differently, and ICA can be used to...”
- The Inhibition of Antibiotic Production in Streptomyces coelicolor Over-Expressing the TetR Regulator SCO3201 IS Correlated With Changes in the Lipidome of the Strain
Zhang, Frontiers in microbiology 2020 - “...putative siderophore encoded by sco5799 - sco5801 as well as the PKS encoding genes ( sco1265 - sco1273 ) and ( sco7670 - sco7671 ) was also down-regulated in M145/pWHM3- sco3201 ( Figure 3 ). The transcriptional activity of genes of the coelibactin cluster ( sco7681...”
- Cytosolic copper is a major modulator of germination, development and secondary metabolism in Streptomyces coelicolor
González-Quiñónez, Scientific reports 2019 - “...0.3 SCO0494 Coelichelin biosynthesis 0.5 0.5 0.7 1.4 SCO0495 Coelichelin biosynthesis 0.7 0.6 0.6 1.6 SCO1265 Aromatic polyketide biosynthesis 0.8 0.9 1.8 1.9 SCO1867 5-Hydroxyectoine biosynthesis 0.4 1.3 1.3 0.4 SCO2782 Desferrioxamine biosynthesis 0.6 1.4 0.7 2.7 SCO3218 CDA biosynthesis 1.8 1 3.6 0.5 SCO3221 CDA...”
W8FKE7 Lipase from Bacillus pumilus
32% identity, 33% coverage
- Structure and Mechanism of a Cold-Adapted Bacterial Lipase
van, Biochemistry 2022 - “...Methods Protein Production A synthetic Bacillus pumilus lipase gene construct corresponding to UniProt accession ID W8FKE7 was codon-optimized for expression in Escherichia coli and synthesized by GenScript. The mature peptide sequence, excluding the signal peptide, was subcloned into pET22b with cytosolic expression. The expression vector containing...”
- ThermoSlope: A Software for Determining Thermodynamic Parameters from Single Steady-State Experiments
Lund, Molecules (Basel, Switzerland) 2021 - “...Production The DNA sequence encoding residues 35215 of the mature Bacillus pumilus Lipase L5 (UniProtKB W8FKE7) was optimised for E. coli expression, synthesised and subcloned into pET-22b(+) within the NdeI/XhoI sites by GenScript Biotech (Leiden, The Netherlands). The plasmid was transformed into NiCo21(DE3) chemically competent E.coli...”
7r1kA / W8FKE7 Phosphorylated bacillus pumilus lipase a
32% identity, 32% coverage
- Ligand: oxaloacetate ion (7r1kA)
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 789,361 different protein sequences to 1,256,019 scientific articles. Searches against EuropePMC were last performed on January 10 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory