PaperBLAST
PaperBLAST Hits for reanno::acidovorax_3H11:Ac3H11_1140 2-methylisocitrate dehydratase (EC 4.2.1.99) (Acidovorax sp. GW101-3H11) (980 a.a., MATSRKSSPQ...)
Show query sequence
>reanno::acidovorax_3H11:Ac3H11_1140 2-methylisocitrate dehydratase (EC 4.2.1.99) (Acidovorax sp. GW101-3H11)
MATSRKSSPQPARHAFANTLKSFRTASGKDGQFYSLPALAKQFPEIKRLPVSIRIVLESV
LRNCDGRKVTPEHVEQLARWAPNAERKDEIPFVVSRVVLQDFTGVPLLADLAAMRSVAAK
LGKNPKKIEPLVPVDLVVDHSIMIDHYGKKNSLDLNMKLEFQRNRERYEFMKWGMQAFDT
FGVVPPGFGIVHQVNLEYLARGVHKRKDGVFYPDTLVGTDSHTTMINGIGVVGWGVGGIE
AEAAMLGQPVYFLTPDVVGFEMTGRLREGVTATDLVLTVTELLRKHKVVGKFVEFFGEGT
RTLALPDRATIGNMAPEYGATMGFFPVDEKTIDYFQGTGRTKAEIEAFEAYFKAQGLFGV
PLAGEVDYSQVVTLDLGSVTPSLAGPKRPQDRIELGQVSRQFADLFSQPAAHNGFNRPAE
LLHTRFHIHRAAEVVADVTPDGKPTPAGAPRSVVEMEANKPALATAHAEARSATLPARGA
DPTVGNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVQPHIKTSLAPGSRIVTE
YLSETGLLPYLEKLGFSIAGYGCTTCIGNAGDLTPELNEAITQNDLVCAAVLSGNRNFEA
RIHPNLKANFLASPPLVVAYAIAGTVLKDLMTEPVGQGKGGKDIYLGDIWPSSDEVHALL
KFAMKGKAFRDNYAKVATDPGKLWEKIQGVSGTAYTWPASTYIAEPPFFAQFALEKGANK
ASGTRGEGQKDAQLPSVMGARIMALFGDSITTDHISPAGSIKESSPAGQWLLQHGVQKAD
FNSYGARRGNHDVMVRGTFANVRIKNLMIPPTADGSREEGGVTVFQNEGALQGEKMFIFD
AAMQYMAQGTPTVVFAGEEYGTGSSRDWAAKGTQLLGIKAVVARSFERIHRSNLVGMGVL
PLQFKAGDSWETLGLTGNEVIDVLPDPALTPQSDARLVIRRADGTVREVVVTLRIDTPIE
VDYYRAGGILPFVLRQLLEG
Running BLASTp...
Found 262 similar proteins in the literature:
Ac3H11_1140 2-methylisocitrate dehydratase (EC 4.2.1.99) from Acidovorax sp. GW101-3H11
100% identity, 100% coverage
- mutant phenotype: Specifically important for utilizing Sodium propionate. Automated validation from mutant phenotype: the predicted function (4.2.1.99) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
H16_A2638 aconitate hydratase from Ralstonia eutropha H16
H16_A2638 aconitate hydratase AcnA from Cupriavidus necator H16
72% identity, 98% coverage
- A novel A3 group aconitase tolerates oxidation and nitric oxide
Doi, The Journal of biological chemistry 2015 - “...102504 Oant_0105 SJA_C1-23180 36 3 30 1 33 2 26 1 AcnA3 BB1844 Daci_2441 H16_A2638 107 3 82 3 73 9 74 3 103 4 76 4 69 10 67 4 ECDH10B_1393 PA1562 44 3 40 1 40 2...”
- “...PAO1 AcnB (PA1787) R. eutropha NBRC 102504 AcnA (H16_A2638) R. eutropha NBRC 102504 AcnB (H16_B0568) R. eutropha NBRC 102504 AcnM (H16_A1907) S. japonicum UT26S...”
- Detection of phase-dependent transcriptomic changes and Rubisco-mediated CO2 fixation into poly (3-hydroxybutyrate) under heterotrophic condition in Ralstonia eutropha H16 based on RNA-seq and gene deletion analyses
Shimizu, BMC microbiology 2013 - “...of PdhA1 and PdhL, respectively, were barely expressed throughout cultivation. gltA (H16_A2627), acnA and acnB (H16_A2638 and H16_B0568, respectively), and icd1 and icd2 (H16_A3056 and H16_B1931, respectively), which encode enzymes for the conversion of C 6 -acids in TCA cycle, were highly expressed in the growth...”
BCAM0961 aconitate hydratase from Burkholderia cenocepacia J2315
71% identity, 98% coverage
- Low iron-induced small RNA BrrF regulates central metabolism and oxidative stress responses in Burkholderia cenocepacia
Sass, PloS one 2020 - “...10 ] BCAM0969 sdhA Succinate dehydrogenase flavoprotein subunit -2.25 n.s. [ 5 , 10 ] BCAM0961 acnA [Fe-S]-dependent aconitate hydratase A -4.22 n.s. [ 10 , 35 ] BCAL2287 fumA [Fe-S]-dependent class I fumarate hydratase -5.38 1.10 [ 10 , 35 ] BCAL2908 fumC Class II...”
- Elucidation of the mechanism behind the potentiating activity of baicalin against Burkholderia cenocepacia biofilms
Slachmuylders, PloS one 2018 - “...lyase - -1.9 TCA cycle BCAM0972 ( gltA ) Type II citrate synthase - 1.8 BCAM0961 ( acnA ) Aconitate hydratase - 1.7 BCAL1215 ( lpdV ) Dihydrolipoamide dehydrogenase - 1.5 BCAL1517 ( odhL ) Dihydrolipoamide dehydrogenase - 1.7 BCAM1250 Putative acetyl-CoA hydrolase/transferase 1.6 1.5 BCAM0970...”
- Biofilm-grown Burkholderia cepacia complex cells survive antibiotic treatment by avoiding production of reactive oxygen species
Van, PloS one 2013 - “...GGCCGACCGCTCGAAGATCA CGCCTTGTCGTTCGCTTCCG BCAL2118 Isocitrate lyase GCACCGTACGCCGACCTGAT GCTTGCCCGGGAACTGCTTG BCAM1588 Isocitrate lyase TCCTTGCCCGCTGCCTTCAT ATCCCGAACGCGAAGCTGGT Tricarboxylic acid cycle BCAM0961 Aconitate hydratase CTCGGCCAGCCGGTGTACTT CAGCGACTTCGTGCCTTCGC BCAM2701 Aconitate hydratase CGGTGATCCAGGCGCAGGAT CAGGCGCATCCACGATGCAC BCAM1833 Aconitate hydratase/methylisocitrate dehydratase GCATGCTGACGGTCGCCAAG GGTCGGGCAGGCCTTCGATT BCAL2735 Isocitrate dehydrogenase CGCTGAAGCCGGAAGCGAAC ACCGCGCTGCCCTTGATCTT BCAL2736 Isocitrate dehydrogenase ATCAAGGGGCCGCTCACGAC GGCGCAGGCACACGTAGAGA BCAL1517 Dihydrolipoamide dehydrogenase GAAGTGAGCGGCGAGGGTGA TCGGCACCGAGTCGAACGTC...”
- “...1.6 BCAL0813 RNA polymerase factor sigma 54 1.5* BCAL1949 Glyoxylate carboligase 1.5 Tricarboxylic acid cycle BCAM0961 Aconitate hydratase 1.1 BCAM2701 Aconitate hydratase 1.3* 1.3 BCAM1833 Aconitate hydratase/methylisocitrate dehydratase 1.5* 1.1 BCAL2735 Isocitrate dehydrogenase 1.3* 2.5* BCAL2736 Isocitrate dehydrogenase 1.4 1.5 BCAL1515 -ketoglutarate dehydrogenase E1 2.0* BCAL1516...”
- Proteomic profiling of Burkholderia cenocepacia clonal isolates with different virulence potential retrieved from a cystic fibrosis patient during chronic lung infection
Madeira, PloS one 2013 - “...<0.001 160 Q59097 BCAL2209 aceE Pyruvate dehydrogenase E1 component 3.4 1.8 0.5 <0.001 10 Q0BAG3 BCAM0961 acnA Aconitate hydratase 3.0 1.9 0.6 0.008 13 A2W536 BCAL3389 tktA Transketolase 1 1.5 1.3 0.8 0.009 254 A2W536 BCAL3389 tktA Transketolase 1 0.5 0.5 1.0 0.014 38 Q1BY10 BCAL2934...”
- Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite
Peeters, BMC genomics 2010 - “...- NS Genes involved in the repair of iron-sulfur clusters and iron-sulfur cluster containing proteins BCAM0961 Aconitase A ( acnA ) NS - - - NS BCAM1833 Aconitase B ( acnB ) 5.1 2.7 3.5 3.1 2.1 BCAL2192 Conserved hypothetical protein ( iscX ) 2.4 -...”
- “...[ 39 , 49 ]. However, in the present study no increased expression of acnA (BCAM0961) was observed, while the number of mRNA transcripts of acnB (BCAL1833) increased both in the H 2 O 2 - and in the NaOCl-treated biofilms; the data obtained for the...”
BB1844 putative aconitate hydratase from Bordetella bronchiseptica RB50
70% identity, 98% coverage
- A novel A3 group aconitase tolerates oxidation and nitric oxide
Doi, The Journal of biological chemistry 2015 - “...NBRC 102504 Oant_0105 SJA_C1-23180 36 3 30 1 33 2 26 1 AcnA3 BB1844 Daci_2441 H16_A2638 107 3 82 3 73 9 74 3 103 4 76 4 69 10 67 4 ECDH10B_1393 PA1562 44 3 40 1...”
- “...Sphingobium japonicum (SJA_C1-23180), Bordetella bronchiseptica (BB1844), Delftia acidovorans (Daci_2441), Ralstonia eutropha (H16_A2683), E. coli...”
BPSS1726 aconitate hydratase from Burkholderia pseudomallei K96243
71% identity, 98% coverage
Rmet_2492 aconitate hydratase AcnA from Cupriavidus metallidurans CH34
Rmet_2492 aconitate hydratase 1 from Ralstonia metallidurans CH34
71% identity, 98% coverage
- The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments
Janssen, PloS one 2010 - “...CitA (Rmet_2481, Rmet_4144, Rmet_4268, Rmet_4946, and Rmet_5380) and citrate to D-isocitrate via aconitase AcnA (Rmet_1585, Rmet_2492, Rmet_4240, and Rmet_5296). We note that D-isocitrate, as for full TCA cycles, is transfigured into 2-ketoglutarate by isocitrate dehydrogenase ICDH (Rmet_2895, Rmet_3729). The carbon skeleton of 2-ketoglutarate then is used,...”
BP2014 putative aconitate hydratase from Bordetella pertussis Tohama I
Q7VX12 Aconitate hydratase from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
70% identity, 98% coverage
- In Vivo Gene Essentiality and Metabolism in Bordetella pertussis
Gonyar, mSphere 2019 - “...to reactions within the tricarboxylic acid cycle ( Fig.3 ). The two nonessential genes ( BP2014 and BP2021 ) are annotated as aconitate hydratases (responsible for interconversion of citrate and isocitrate), and these genes may be functionally redundant. Together, these data suggest not only a functional...”
- A Functional Tricarboxylic Acid Cycle Operates during Growth of Bordetella pertussis on Amino Acid Mixtures as Sole Carbon Substrates
Izac, PloS one 2015 - “...recA-F TCATCGCCGAAATGCAGAAG recA (BP2546) recA-R CTTCATGCGGATCTGGTTGA gltA (BP2358) gltA-F ATCACACGATGGTCAACGAG gltA (BP2358) gltA-R CTTGTACTCTTCACACGGGG acnA (BP2014) acnA-F TGTCAGTTCTATTCGCTGCC acnA (BP2014) acnA-R CAGCTTCATGTTCAGGTCGA acnB (BP2021) acnB-F CCAAGCAAGGCAAGAAGAAC acnB (BP2021) acnB-R CTCGTCTTCATAGCCGTTGG icd (BP2488) icd-F TCATGAAGTTCACGGAAGGC icd (BP2488) icd-R GAGATGTAGTCGCCGTTCAG Enzymatic assays All the following steps were achieved...”
- “...acetyl-CoA+ oxaloacetate citrate gltA BP2358 type II citrate synthase citrate isocitrate (intermediary: cis -aconitate) acnA BP2014 aconitate hydratase citrate isocitrate (intermediary: cis -aconitate) acnB BP2021 bifunctional aconitate hydratase 2 isocitrate -ketoglutarate icd BP2488 isocitrate dehydrogenase -ketoglutarate succinyl-CoA odhB BP1125 dihydrolipoamide succinyltransferase -ketoglutarate succinyl-CoA sucA BP1124 2-oxoglutarate...”
- Role of Bordetella pertussis RseA in the cell envelope stress response and adenylate cyclase toxin release
Hanawa, Pathogens and disease 2013 - “...E -dependent promoters and cyaA and cyaB promoters The DNA regions upstream of the BP0893, BP2014 ( acnA ), BP3218, and BP3748 ( rpoH ) genes of B. pertussis UT25Sm1 were amplified by PCR from genomic DNA using the primer pairs: BP0893-EcoRI and BP0893-XbaI, BP2014-EcoRI and...”
- “...promoter sequences upstream of the genes of unknown function, BP0893 and BP3218, as well as BP2014 (annotated as acnA , encoding a putative aconitase A) and BP3748, an ortholog of rpoH . In characterized bacterial systems, rpoH expression is regulated by E ( Raivio & Silhavy,...”
- A gel-free proteomic-based method for the characterization of Bordetella pertussis clinical isolates
Williamson, Journal of microbiological methods 2012 - “...accA 35 C 17 (4) 29 (6) 24 (5) 17 (4) Metabolism Aconitate hydratase NP_880684 BP2014 acnA 99 C 5 (3) 5 (3) 3 (2) 4 (2) Metabolism Acriflavine resistance protein B NP_879779 BP0985 acrB 116 CM 6 (4) 3 (2) 5 (3) 3 (2) Drug...”
- Bordetella pertussis autotransporter Vag8 binds human C1 esterase inhibitor and confers serum resistance
Marr, PloS one 2011 - “...Gene product Probability Based Mowse Score BP338 BP347 BP2315 vag8 autotransporter Vag8 2011 not detected BP2014 acnA Putative aconitate hydratase 1265 1251 BP1112 bipA putative OM ligand binding protein (BipA) 1185 not detected BP0197 gcvP glycine cleavage system P protein not detected 238 BP2497 putative zinc...”
- Antibody Specificity Following a Recent Bordetella pertussis Infection in Adolescence Is Correlated With the Pertussis Vaccine Received in Childhood
Raeven, Frontiers in immunology 2019 - “...92 U4 TonB-dependent outer membrane receptor ABC47_15525 A0A1P9PLY0 77.8 6.6 40.4 23 Aconitate hydratase acnA Q7VX12 97.7 7.4 30.6 26 II U5 ** U6 ** U7 ** U8 ** U9 ** U10 putative peptidoglycan associated protein based on 47 strip Dsc of 2-odc *** odhB Q7VZ17...”
Q2T7J6 Aconitate hydratase from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CCUG 48851 / CIP 106301 / E264)
BTH_II0654 aconitate hydratase 1 from Burkholderia thailandensis E264
71% identity, 98% coverage
Hsero_2979 aconitate hydratase AcnA from Herbaspirillum seropedicae SmR1
71% identity, 98% coverage
Q0BAG3 Aconitate hydratase from Burkholderia ambifaria (strain ATCC BAA-244 / DSM 16087 / CCUG 44356 / LMG 19182 / AMMD)
70% identity, 98% coverage
A0A090AMG4 aconitate hydratase (EC 4.2.1.3) from Achromobacter denitrificans (see paper)
69% identity, 98% coverage
Dgeo_1682 aconitate hydratase 1 from Deinococcus geothermalis DSM 11300
53% identity, 97% coverage
Amuc_0904 aconitate hydratase 1 from Akkermansia muciniphila ATCC BAA-835
53% identity, 98% coverage
XALc_1349 probable aconitate hydratase 1 protein from Xanthomonas albilineans
54% identity, 97% coverage
rpfa / CAA05170.1 aconitase from Xanthomonas campestris (see paper)
54% identity, 98% coverage
Mal48_48270 aconitate hydratase AcnA from Thalassoglobus polymorphus
53% identity, 98% coverage
YP_091630 CitB from Bacillus licheniformis DSM 13
51% identity, 99% coverage
lmo1641 highly similar to aconitate hydratases from Listeria monocytogenes EGD-e
NP_465166 aconitate hydratase from Listeria monocytogenes EGD-e
51% identity, 97% coverage
- Genome-wide identification of Listeria monocytogenes CodY-binding sites
Biswas, Molecular microbiology 2020 - “...800 nM was detected for the regulatory regions of the ptb (lmo1369, LMRG_RS06810, LMRG_00819), citB (lmo1641, LMRG_RS08180, LMRG_01325) , or glnR (lmo1298, LMRG_RS06455, LMRG_00748) genes ( Fig. 5G I ). None of these three regions were found in our 2,311-region dataset ( Dataset S1 ); the...”
- c-di-AMP modulates Listeria monocytogenes central metabolism to regulate growth, antibiotic resistance and osmoregulation
Whiteley, Molecular microbiology 2017 - “...; citZ , lmo1567 , LMRG_01400 ; citC , lmo1566 , LMRG_01401 ; citB , lmo1641 , LMRG_01325 . L. monocytogenes strain construction Strains were constructed by standard methods ( Reniere et al. , 2016 ). Plasmids were introduced to L. monocytogenes by transconjugation using donor...”
- “...transduced with lysate from DP-L6378 This study DP-L6540 citB In frame deletion of citB ( lmo1641 ), made with DP-E6409 This study DP-L6541 dacA-kanR citB DP-L6540 transduced with lysate from DP-L6378 This study DP-L6401 citZhimar1 Transposon mutation in citZ (LMRG_01400143, lmo1567) ( Sureka et al. ,...”
- Global transcriptional analysis of spontaneous sakacin P-resistant mutant strains of Listeria monocytogenes during growth on different sugars
Tessema, PloS one 2011 - “...3 lmo1567 ( citZ ) citrate synthase subunit II 0.8 10 5 0.8 10 4 lmo1641 aconitate hydratases 0.8 10 4 0.9 10 5 lmo1957 ( fhuG ) ferrichrome ABC transporter (permease) 0.6 10 4 0.7 10 3 lmo1958 ( fhuB ) ferrichrome ABC transporter (permease)...”
- Transcriptome analysis of alkali shock and alkali adaptation in Listeria monocytogenes 10403S
Giotis, Foodborne pathogens and disease 2010 - “...LMOf2365_2351 lmo2384 LMOf2365_1426 lmo1406 lmo0210 lmo2458 lmo1567 lmo1641 lmo2122 lmo1293 lmo1538 lmo1407 LMOf2365_1073 lmo1053 lmo2124 4.67 8.21 10.4 8.09...”
- CcpC-dependent regulation of citB and lmo0847 in Listeria monocytogenes.
Kim, Journal of bacteriology 2006 - GeneRIF: regulated by CcpC in media containing an excess of glucose and glutamine
LMRG_RS08180 aconitate hydratase AcnA from Listeria monocytogenes 10403S
LMRG_01325 aconitate hydratase 1 from Listeria monocytogenes 10403S
52% identity, 97% coverage
- Genome-wide identification of Listeria monocytogenes CodY-binding sites
Biswas, Molecular microbiology 2020 - “...nM was detected for the regulatory regions of the ptb (lmo1369, LMRG_RS06810, LMRG_00819), citB (lmo1641, LMRG_RS08180, LMRG_01325) , or glnR (lmo1298, LMRG_RS06455, LMRG_00748) genes ( Fig. 5G I ). None of these three regions were found in our 2,311-region dataset ( Dataset S1 ); the probed...”
- Genome-wide identification of Listeria monocytogenes CodY-binding sites
Biswas, Molecular microbiology 2020 - “...was detected for the regulatory regions of the ptb (lmo1369, LMRG_RS06810, LMRG_00819), citB (lmo1641, LMRG_RS08180, LMRG_01325) , or glnR (lmo1298, LMRG_RS06455, LMRG_00748) genes ( Fig. 5G I ). None of these three regions were found in our 2,311-region dataset ( Dataset S1 ); the probed sequences...”
- c-di-AMP modulates Listeria monocytogenes central metabolism to regulate growth, antibiotic resistance and osmoregulation
Whiteley, Molecular microbiology 2017 - “..., lmo1567 , LMRG_01400 ; citC , lmo1566 , LMRG_01401 ; citB , lmo1641 , LMRG_01325 . L. monocytogenes strain construction Strains were constructed by standard methods ( Reniere et al. , 2016 ). Plasmids were introduced to L. monocytogenes by transconjugation using donor E. coli...”
- Identification of Listeria monocytogenes determinants required for biofilm formation
Alonso, PloS one 2014 - “...dehydrogenase component E1 lmrg_00823 980.35 3 2 Xanthine ribosyltransferase lmrg_01032 900.21 1 1 Aconitate hydratase lmrg_01325 920.12 1 1 Homoserine dehydrogenase lmrg_01700 840.35 1 1 Peptidoglycan N-acetylglucosamine deacetylase lmrg_00107 990.06 1 1 Adenylosuccinate synthetase lmrg_02457 890.07 1 1 Gene Regulation Signal peptidase I lmrg_00721 1000.34 2...”
GYA98_RS08030 aconitate hydratase AcnA from Bacillus velezensis
51% identity, 98% coverage
- Comparative transcriptome analysis reveals the biocontrol mechanism of Bacillus velezensis E68 against Fusarium graminearum DAOMC 180378, the causal agent of Fusarium head blight
Liang, PloS one 2023 - “...these terms included the enzymes of the TCA cycle: 2 citrate synthases (GYA98_RS03820, 12240), aconitase (GYA98_RS08030), isocitrate dehydrogenase (GYA98_RS12235), 2-oxoglutarate dehydrogenase (GYA98_RS08685, 08690), succinate dehydrogenase (GYA98_RS11900, 11905), succinyl-CoA ligase (GYA98_RS07090, 07095) and fumarate dehydrogenase (GYA98_RS14210), as well as a citrate/malate transporter (GYA98_RS17095) ( S2 Table )....”
citB / P09339 aconitate hydratase (bifunctional aconitase) (EC 4.2.1.99; EC 4.2.1.3) from Bacillus subtilis (strain 168) (see 21 papers)
ACNA_BACSU / P09339 Aconitate hydratase A; ACN; Aconitase; Aconitate/2-methylaconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.- from Bacillus subtilis (strain 168) (see 7 papers)
P09339 aconitate hydratase (EC 4.2.1.3) from Bacillus subtilis (see paper)
NP_389683 aconitate hydratase (bifunctional aconitase) from Bacillus subtilis subsp. subtilis str. 168
BSU18000 aconitate hydratase from Bacillus subtilis subsp. subtilis str. 168
51% identity, 100% coverage
- function: Involved in both the tricarboxylic acid (TCA) and methylcitric acid cycles (PubMed:28956599). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate (PubMed:23354745, PubMed:3110133). Also catalyzes the rehydration of 2- methyl-cis-aconitate to produce 2-methylisocitrate (PubMed:28956599). The apo form of AcnA functions as a RNA-binding regulatory protein which plays a role in the regulation of citrate concentration and in the sporulation. To prevent the accumulation of excessive levels of citrate, it binds near the 5' end of the citZ mRNA, decreasing its stability and thereby limiting the concentration of citrate synthase in the cell. Aconitase also binds to the gerE transcript late in sporulation and stabilizes it for translation, thereby increasing the rate and level of GerE protein accumulation (PubMed:10468622, PubMed:16923907, PubMed:23354745, PubMed:9393699).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
catalytic activity: 3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis-aconitate + H2O (RHEA:57500)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer.
disruption phenotype: Cells lacking this gene show a dramatic increase in the concentration of citrate. This accumulation of citrate prevents sporulation due to chelation by citrate of divalent cations required for proper functioning of the Spo0A-initiated phosphorelay. - Two roles for aconitase in the regulation of tricarboxylic acid branch gene expression in Bacillus subtilis.
Pechter, Journal of bacteriology 2013 - GeneRIF: Together, these data suggest that wild-type aconitase binds to and destabilizes the citZ transcript in order to maintain proper cell homeostasis by preventing the overaccumulation of citrate.
- CitB mutation increases the alkaline protease productivity in Bacillus subtilis.
Gao, The Journal of general and applied microbiology 2010 (PubMed)- GeneRIF: Alkaline protease production in Bacillus subtilis may be influenced by aconitase activity.
- Proteomic Response of Bacillus subtilis Spores under High Pressure Combined with Moderate Temperature and Random Peptide Mixture LK Treatment
Pang, Foods (Basel, Switzerland) 2022 - “...Table 2 , the TCA cycle pathway included 2-oxoglutarate dehydrogenase E1 component (P23129), aconitate/2-methylaconitate hydratase (P09339), malate dehydrogenase (P49814), pyruvate carboxylase (Q9KWU4), dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (A0A6A8FKK9), phosphoenolpyruvate carboxykinase (ATP) (A0A7U5BTG7), succinate-CoA ligase (ADP-forming) subunit alpha (A0A5F2KMI5), succinate dehydrogenase flavoprotein subunit (A0A3N6CX89), and...”
- “...is the first phase in the conversion of 2-oxoglutarate to succinyl-CoA and CO. Aconitate/2-methylaconitate hydratase (P09339) is capable of catalyzing the reversible isomerization of citrate to isocitrate via cis -aconitate and the rehydration of 2-methyl-cis-aconitate to produce 2-methylisocitrate, which is an essential step in the tricarboxylic...”
- Correction: Comprehensive Analysis of Temporal Alterations in Cellular Proteome of Bacillus subtilis under Curcumin Treatment
Reddy, PloS one 2015 - “...cycle P39120 Citrate synthase 2 CitZ 31.18 11 1.20 3.99 7.65 7 1.448 3.869 6.546 P09339 Aconitate hydratase CitB 33.22 25 1.14 3.94 4.56 12 1.449 4.064 3.887 P39126 Isocitrate dehydrogenase [NADP] Icd 44.21 20 1.10 3.06 3.53 9 1.003 3.302 2.885 P23129 2-oxoglutarate dehydrogenase E1...”
- Proteomics analyses of Bacillus subtilis after treatment with plumbagin, a plant-derived naphthoquinone
Reddy, Omics : a journal of integrative biology 2015 - “...iron-sulfur subunit P39126 Isocitrate dehydrogenase [NADP] OS P09339 Aconitate hydratase OS P39120 Citrate synthase 2 OS Electron transport P94551 Electron...”
- Comprehensive analysis of temporal alterations in cellular proteome of Bacillus subtilis under curcumin treatment
Reddy, PloS one 2015 - “...U-10 * P39121 Deoxyribose-phosphate aldolase DeoC 23.47 13 1.72 NS NS 611 524 0.041 U-11 P09339 Aconitate hydratase CitB 99.61 28 4.13 4.56 3.88 789 664 0.0015 $ This is a partial list having selected candidates with >1.5 fold change and complete list is provided in...”
- “...cycle P39120 Citrate synthase 2 CitZ 31.18 11 1.20 3.99 7.65 7 1.621 3.706 8.131 P09339 Aconitate hydratase CitB 33.22 25 1.14 3.94 4.56 12 2.032 4.297 4.724 P39126 Isocitrate dehydrogenase [NADP] Icd 44.21 20 1.10 3.06 3.53 9 2.008 2.694 3.139 P23129 2-oxoglutarate dehydrogenase E1...”
- A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study
Albaum, Proteome science 2011 - “...<0.000001 <0.000001 2391 O31663 0.000143 >0.99 >0.99 0.378199 340 O34633 0.000185 >0.99 >0.99 0.025892 433 P09339 0.000203 0.244699 >0.99 0.040871 619 O07516 0.000230 >0.99 >0.99 0.955961 370 P24327 0.000234 >0.99 <0.000001 <0.000001 1692 P37494 0.000244 >0.99 >0.99 0.932235 241 P32399 0.000267 >0.99 >0.99 0.045315 388 P39912...”
- “...>0.99 0.028009 845 P42971 0.000473 >0.99 >0.99 0.032372 475 P23447 0.078522 >0.99 0.721913 0.036485 459 P09339 0.000203 0.244699 >0.99 0.040871 619 P37870 0.017921 0.000748 >0.99 0.041718 1113 O32047 0.178170 >0.99 <0.000001 0.042466 665 P12042 0.002703 >0.99 >0.99 0.043005 699 P32399 0.000267 >0.99 >0.99 0.045315 388 P08066...”
- Identification of an RNA sponge that controls the RoxS riboregulator of central metabolism in Bacillus subtilis
Durand, Nucleic acids research 2021 - “...acetate, fatty acids SpoVS BSU16980 1.57 FsrA Unknown spore coat assembly, spore core dehydratation CitB BSU18000 1.40 FsrA FsrA Aconitase, trigger enzyme TCA cycle NadK2/ PpnKB/YtdI BSU29540 1.14 RoxS RoxS ATP-NAD kinase NADP biosynthesis YrhF BSU27210 1.07 RoxS Unknown EtfA BSU28520 0.95 RoxS and FsrA RoxS...”
- Comparison of Bacillus subtilis transcriptome profiles from two separate missions to the International Space Station
Morrison, NPJ microgravity 2019 - “...2.89 3.06 2.67 2.71 Inhibitor of entry into sporulation via KinB or KinC AbrB, SigD BSU18000 citB 1.83 1.99 1.17 1.18 aconitase CcpA, CcpC, CitB, CodY, FsrA, SigA BSU21330 yomK 1.47 1.58 1.90 1.93 Unknown SP prophage BSU21420 bhlA 1.09 1.34 1.05 1.28 Holin-like protein SP...”
- Secondary structural entropy in RNA switch (Riboswitch) identification
Manzourolajdad, BMC bioinformatics 2015 - “...0.3567 393 ywcI BSU38080 0.851 46 1926523 1926679 forward BSU17950 yneJ -1482 0.3949 79 citB BSU18000 0.851 47 1017271 1017427 forward BSU09400 spoVR -139 0.363 1649 lytE BSU09420 0.85 48 1493595 1493751 reverse BSU14250 yknT -729 0.4522 1649 ykuT BSU14210 0.85 49 2477743 2477899 forward BSU23830...”
- “...0.3567 393 ywcI BSU38080 0.851 24 1926523 1926679 forward BSU17950 yneJ -1482 0.3949 79 citB BSU18000 0.851 25 1017271 1017427 forward BSU09400 spoVR -139 0.363 1649 lytE BSU09420 0.85 26 2769617 2769773 reverse BSU27160 cypB -4194 0.3185 1021 yrhP BSU27100 0.849 27 2739991 2740147 reverse BSU26830...”
PXO_00064 aconitate hydratase 1 from Xanthomonas oryzae pv. oryzae PXO99A
53% identity, 97% coverage
BRADO0393 aconitate hydratase from Bradyrhizobium sp. ORS278
52% identity, 96% coverage
- A proteomic approach of bradyrhizobium/aeschynomene root and stem symbioses reveals the importance of the fixA locus for symbiosis
Delmotte, International journal of molecular sciences 2014 - “...transport protein (LivG), BRADO5083 encoding a conserved hypothetical protein, BRADO1684/1685 encoding the hydrogenase uptake HupS/L, BRADO0393 encoding aconitate hydratase (AcnA), BRADO4479 encoding a putative porin and BRADO4132 encoding an abundant conserved hypothetical protein annotated as containing surface antigen domains were selected for mutagenesis. We used pVO155...”
- “...In order to construct mutant strains altered in fixA (BRADO5386), livG (BRADO0979), hupS (BRADO1684), acnA (BRADO0393), BRADO4132, BRADO5083 and BRADO4479, DNA fragments were amplify from ORS278 DNA using the following primer pairs: BRADO5386F (5-GTGCGTCGA CCTCATCAATCCCTACGACCTGTTC-3)/BRADO5386F (5-CCGTCTAGACCTCGTTGATCTTG GCCACATAG-3); BRADO0979F (5-CGGTGTCGACGGCGCCGGCAAGACCACACTGTTC-3)/ BRADO0979R (5-GGTCTTCTAGACCACCTCCTCGAGATTGAGATAG-3); BRADO1684F (5-CTG GCGTCGACGCGCATTGCCAATGCGCTGGAAAC-3)/BRADO1684R (5-GCCATCTAGACCTCGG CCATCACCTTCAACTTCTC-3);...”
DW68_010115 aconitate hydratase AcnA from Pseudomonas mendocina S5.2
53% identity, 97% coverage
- Phenotypic and genomic survey on organic acid utilization profile of Pseudomonas mendocina strain S5.2, a vineyard soil isolate
Chong, AMB Express 2017 - “...I fumA 1,503,2281,504,751 1524 DW68_014875 Fumarate hydratase class II fumC 3,205,0053,206,399 1395 Citric acid (citrateisocitrate) DW68_010115 Aconitate hydratase A acnA 2,182,6292,185,370 2742 DW68_013280 Aconitate hydratase B acnB 2,870,4272,873,027 2601 Besides, an operon associated with lactic acid utilization encoding putative NAD-independent lactate dehydrogenase (iLDH) activity was also...”
HP15_3433 Aconitate hydratase (EC 4.2.1.3) from Marinobacter adhaerens HP15
52% identity, 98% coverage
- mutant phenotype: Specifically important for utilizing Potassium acetate. Automated validation from mutant phenotype: the predicted function (4.2.1.3) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
GL4_0055 aconitate hydratase AcnA from Methyloceanibacter caenitepidi
51% identity, 97% coverage
Mal4_15300 aconitate hydratase AcnA from Maioricimonas rarisocia
54% identity, 95% coverage
ACNA_THET8 / Q5SMF6 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
YP_143992 aconitate hydratase (aconitase) from Thermus thermophilus HB8
52% identity, 98% coverage
- function: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis- aconitate. Also able to catalyze the hydration of cis-homoaconitate to yield (R)-homocitrate, but with a lower efficiency (PubMed:23106124). Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnA functions as a RNA- binding regulatory protein (By similarity).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
catalytic activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis- aconitate + H2O (RHEA:17941)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer. - The fungal α-aminoadipate pathway for lysine biosynthesis requires two enzymes of the aconitase family for the isomerization of homocitrate to homoisocitrate
Fazius, Molecular microbiology 2012 - “...from the thermophilic bacterium T. thermophilus . A protein annotated as aconitate hydratase (protein accession YP_143992) was identified and the gene was amplified from genomic DNA and cloned into the pET expression vector for recombinant production in E. coli . In summary, seven aconitase genes were...”
XF_0290 aconitate hydratase AcnA from Xylella fastidiosa 9a5c
XF0290 aconitase from Xylella fastidiosa 9a5c
52% identity, 97% coverage
- Transcriptome and Secretome Analyses of Endophyte Methylobacterium mesophilicum and Pathogen Xylella fastidiosa Interacting Show Nutrient Competition
Dourado, Microorganisms 2023 - “...0.96 62 - XF9a_00008 XF_0007 Protein of Unknown Function 44 6 0.75 63 - XF9a_00264 XF_0290 Aconitase 98 3 0.78 64 - XF9a_00072 XF_0081 Outer membrane FimD 98 2 0.91 * Number of the locus tag of Xf gene. ** Name assigned to gene and retrieved...”
- Global gene expression under nitrogen starvation in Xylella fastidiosa: contribution of the σ54 regulon
da, BMC microbiology 2010 - “...nuoABCDEFGHIJKLMN ) XF1249 -207 CGGCCCGCAGCATGCT 8.97 hypothetical protein XF1749 -27 TGGCGCGGCGTTTCCT 8.92 MFS transporter (XF1749-48-47-46) XF0290 -30 CGGCACTGCCACTGCA 8.90 aconitate hydratase XF2580 -109 CGGCACGGAGGCGGCA 8.81 30S ribosomal protein S2 XF2639 -43 TGGCGCGCCACTTTCT 8.79 preprotein translocase subunit SecE ( secE-nusG ) XF0177 -161 TGGCCTGCATTTGGCA 8.79 hypothetical protein...”
- The iron stimulon of Xylella fastidiosa includes genes for type IV pilus and colicin V-like bacteriocins
Zaini, Journal of bacteriology 2008 - “...shows that the transcriptional profiles of CDS XF0371 and XF0290 at HIC and of XF0933 and XF1408 at LIC obtained by microarray experiments are in agreement with...”
- “...XF1535 XF2596 XF0942 XF2547 XF2548 XF0290 Citrate synthase Isocitrate dehydrogenase Malate:quinone oxidoreductase Succinyl-CoA synthetase, beta subunit...”
- Expression of putative pathogenicity-related genes in Xylella fastidiosa grown at low and high cell density conditions in vitro
Scarpari, FEMS microbiology letters 2003 (PubMed)- “...HCD conditions. Expressions of ORFs XF2367, XF2362 and XF0290, encoding putative GumD, GumJ and RpfA, respectively, were detected only at HCD conditions,...”
- “...putative identities ORF Putative identity XF0125 XF0287 XF0290 XF0818 XF0928 XF0972 XF1114 XF1115 XF1408 XF1523 XF1524 XF1623 XF1823 XF1851 XF2344 XF2362...”
XP_002524184 aconitate hydratase, cytoplasmic from Ricinus communis
51% identity, 98% coverage
- The aconitate hydratase family from Citrus
Terol, BMC plant biology 2010 - “...Aco2 57 XM_002321090 XP_2321126 POPTRDRAFT_246575 Aco2 5 R. communis XM_002530589 XP_002530635 RCOM_0782740 Aco1 2 XM_002524138 XP_002524184 RCOM_0487910 Aco2 12 XM_002532518 XP_002532564 RCOM_0082520 Aco3 0 V. vinifera XM_002263301 XP_2263337 LOC100242027 Aco1 29 XM_002279224 XP_2279260 LOC100256776 Aco2 18 XM_002278102 XP_2278138 LOC100253811 Aco3 38 G. max BT095399 Glyma01g36750 Aco1...”
MMSR116_03790, MMSR116_RS03750 aconitate hydratase AcnA from Methylobacterium mesophilicum SR1.6/6
51% identity, 98% coverage
- Transcriptome and Secretome Analyses of Endophyte Methylobacterium mesophilicum and Pathogen Xylella fastidiosa Interacting Show Nutrient Competition
Dourado, Microorganisms 2023 - “...0.81 42 - MMSR116_RS05635 MMSR116_05745 30S ribosomal protein S1 RpsA 63 3 43 - MMSR116_RS03750 MMSR116_03790 Aconitate hydratase 97 3 0.60 - 11 MMSR116_RS21075 *** MMSR116_21360 Inorganic pyrophosphatase Ppa 20 8 44 - MMSR116_RS13495 MMSR116_13665 Pyruvate, phosphate dikinase 97 2 - 16 MMSR116_RS19390 *** MMSR116_19640 Adenosylhomocysteinase...”
- “...8 0.81 42 - MMSR116_RS05635 MMSR116_05745 30S ribosomal protein S1 RpsA 63 3 43 - MMSR116_RS03750 MMSR116_03790 Aconitate hydratase 97 3 0.60 - 11 MMSR116_RS21075 *** MMSR116_21360 Inorganic pyrophosphatase Ppa 20 8 44 - MMSR116_RS13495 MMSR116_13665 Pyruvate, phosphate dikinase 97 2 - 16 MMSR116_RS19390 *** MMSR116_19640...”
Poly24_20280 aconitate hydratase AcnA from Rosistilla carotiformis
52% identity, 98% coverage
BAS3408 aconitate hydratase 1 from Bacillus anthracis str. Sterne
GBAA3677 aconitate hydratase 1 from Bacillus anthracis str. 'Ames Ancestor'
51% identity, 97% coverage
P49608 Aconitate hydratase, cytoplasmic from Cucurbita maxima
51% identity, 99% coverage
W8Y6I8 Aconitate hydratase from Bacillus thuringiensis DB27
50% identity, 97% coverage
LOC18055416 aconitate hydratase, cytoplasmic from Citrus x clementina
51% identity, 97% coverage
- Transcriptome analysis of the pulp of citrus fruitlets suggests that domestication enhanced growth processes and reduced chemical defenses increasing palatability
Perez-Roman, Frontiers in plant science 2022 - “...Likewise, genes coding for a series of enzymes acting sequentially, such as aconitate hydratase, ACO3 (LOC18055416, EC:4.2.1.3, Supplementary Figure 4.058 ), one isocitrate dehydrogenase [NADP], IDH1, (LOC18031748, EC:1.1.4.2) and aspartate transaminase, GOT1 (LOC18054901, EC:2.6.1.1, Supplementary Figure 4.017 ), rendering the amino acid glutamate from 2-oxogluarate, were...”
LOC105178516 aconitate hydratase, cytoplasmic from Sesamum indicum
50% identity, 97% coverage
- Discovering favorable genes, QTLs, and genotypes as a genetic resource for sesame (Sesamum indicum L.) improvement
Kefale, Frontiers in genetics 2022 - “...transcription factor 6 LOC105178495 Transcription factor MYB101 LOC105178506 MYB-like transcription factor ETC1 LOC105178507 Isocitrate lyase LOC105178516 Aconitate hydratase LOC105178613 Calcium-binding protein PBP1-like LOC105178537 Transcription factor MYB39-like LOC105178559 Ethylene-responsive transcription factor 4-like LOC105178589 Zinc transporter 8-like LOC105178590 Zinc transporter 8 Source: Teboul et al. (2020) . Teboul...”
- Genetic Architecture Underpinning Yield Components and Seed Mineral-Nutrients in Sesame
Teboul, Genes 2020 - “...transporter (LOC105173161), citrate synthase (LOC105173380), nicotianamine aminotransferase A (LOC105178476), isocitrate lyase (LOC105178507) and aconitate hydratase (LOC105178516). An important, yet poorly investigated aspect is the impact of high seed nutrient concentration on the bio-accessibility level, which can also be affected from the level of anti-nutritional factors. Chelation...”
- “...transcription factor 6 LOC105178495 Transcription factor MYB101 LOC105178506 MYB-like transcription factor ETC1 LOC105178507 Isocitrate lyase LOC105178516 Aconitate hydratase LOC105178613 Calcium-binding protein PBP1-like LOC105178537 Transcription factor MYB39-like LOC105178559 Ethylene-responsive transcription factor 4-like LOC105178589 Zinc transporter 8-like LOC105178590 Zinc transporter 8...”
A9HEZ2 Aconitate hydratase from Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5)
50% identity, 98% coverage
G7JDJ4 Aconitate hydratase from Medicago truncatula
50% identity, 99% coverage
D7T1R6 Aconitate hydratase from Vitis vinifera
49% identity, 100% coverage
CCNA_03781 aconitate hydratase from Caulobacter crescentus NA1000
CC_3667 aconitate hydratase from Caulobacter crescentus CB15
51% identity, 96% coverage
- The BR-body proteome contains a complex network of protein-protein and protein-RNA interactions
Nandana, Cell reports 2023 (secret) - α-Proteobacterial RNA Degradosomes Assemble Liquid-Liquid Phase-Separated RNP Bodies
Al-Husini, Molecular cell 2018 - “...NA1000 acon::acon-Chy Spec R The strain was generated by subcloning the last 500bp of aconitase (CCNA_03781) lacking the stop codon from pacon-YFPC-1plasmid to the Nde1/Kpn1 cut pCHYC-1 plasmid ( Thanbichler et al., 2007 ). The sequencing verified plasmid was transformed into NA1000 cells and cells were...”
- “...Spec R Acon-YFP fusion was generated by cloning the last 500bp of the aconitase gene (CCNA_03781) lacking a stop codon into pYFPC-1 plasmid ( Thanbichler et al., 2007 ). First the last 500 bp of the aconitase gene were amplified by PCR from the NA1000 genome...”
- Global transcriptional response of Caulobacter crescentus to iron availability
da, BMC genomics 2013 - “...reductase subunit (Fe-S cluster) 2.30 CC_3659 CCNA_03774 Citrate lyase beta chain/citryl-CoA lyase subunit 2.00 CC_3667 CCNA_03781 Aconitate hydratase (Fe-S cluster) 2.30 Miscellaneous CC_0566 CCNA_00601 MoxR-like ATPase 2.04 CC_1409 b CCNA_01475 OmpW family outer membrane protein 3.21 CC_1754 b CCNA_01830 TonB-dependent receptor 2.07 CC_2389 CCNA_02472 Cobalt-zinc-cadmium resistance...”
- Global transcriptional response of Caulobacter crescentus to iron availability
da, BMC genomics 2013 - “...Ferredoxin reductase subunit (Fe-S cluster) 2.30 CC_3659 CCNA_03774 Citrate lyase beta chain/citryl-CoA lyase subunit 2.00 CC_3667 CCNA_03781 Aconitate hydratase (Fe-S cluster) 2.30 Miscellaneous CC_0566 CCNA_00601 MoxR-like ATPase 2.04 CC_1409 b CCNA_01475 OmpW family outer membrane protein 3.21 CC_1754 b CCNA_01830 TonB-dependent receptor 2.07 CC_2389 CCNA_02472 Cobalt-zinc-cadmium...”
- Fur controls iron homeostasis and oxidative stress defense in the oligotrophic alpha-proteobacterium Caulobacter crescentus
da, Nucleic acids research 2009 - “...flavoprotein CC_0139 d,e 72 ATTGCGAGTGACACTCAGA 14.96 TonB-dependent receptor CC_2194 d,e 118 ATTGCGATTGATTCTGAGA 14.39 TonB-dependent receptor CC_3667 d acnA 204 AATGAGAACAGCTCTCAAC 14.32 Aconitate hydratase CC_2928 e CC2928-CC2927-CC2926 33 ATTGCGACGCACTCGCAAT 14.05 TonB-dependent receptor CC_3263 d,e bfd 38 GATGAGAATGACACTCAAT 13.96 Hypothetical protein, BFD-like [2Fe-2S] binding domain CC_2193 d 131...”
BC3616 Aconitate hydratase from Bacillus cereus ATCC 14579
50% identity, 97% coverage
- Cysteine Proteome Reveals Response to Endogenous Oxidative Stress in Bacillus cereus
Hamitouche, International journal of molecular sciences 2021 - “...ATLAWK 1.4 NHGVLEDSVTVNLNNPTC 40 GDR BC3773 Pyruvate synthase AAANVGLNPDELAVISGIGC 50 SGR 2.4 NSVKPNWCPGC 16 GDFSVQAAIQR BC3616 Aconitate hydratase VVEEYC 346 K 1.0 BC3833 Succinate--CoA ligase subunit alpha (SucD) LLGPNC 124 PGVITPDEC 133 K 1.2 IKTMEAC 269 GIK GLFETC 297 K BC5387 Phosphate acetyltransferase GC 306 NEEEVYK...”
- Methionine Residues in Exoproteins and Their Recycling by Methionine Sulfoxide Reductase AB Serve as an Antioxidant Strategy in Bacillus cereus
Madeira, Frontiers in microbiology 2017 - “...dehydrogenase 2.43 NA BC5138 Pgk Phosphoglycerate kinase 1.79 NP_834571 BC4898 Pgi Glucose-6-phosphate isomerase 2.30 NP_833346 BC3616 Acn Aconitate hydratase 3.30 Fatty acid and phospholipid NP_833485 BC3761 PlcA 1-phosphatidylinositol phosphodiesterase precursor 1.15 Amino acids NP_830183 BC0344 RocA 1-pyrroline-5-carboxylate dehydrogenase 3.18 NA BC3705 GlnA1 Glutamine synthetase, type I...”
- FlhF Is Required for Swarming Motility and Full Pathogenicity of Bacillus cereus
Mazzantini, Frontiers in microbiology 2016 - “...TM 0.32 1.25 BC1110 Hemolysin, cytotoxin K, CytK Bacterial toxin 77 7 S 0.23 0.92 BC3616 Aconitate hydratase Metabolism 79 21 TM 0.22 1.00 BC1657 Flagellin, Fla Cell motility 86 7 NCS 0.22 1.11 BC0129 Elongation factor-Tu, EF-Tu Translation factor 78 9 0 1.00 BC3161 Collagenase,...”
- “...mutant strain. The mutant also displayed a reduction in the spots corresponding to aconitate hydratase (BC3616; P = 0.0048) and elongation factor-Tu (EF-Tu; P = 0.002), commonly found in the cytoplasm. Contribution of FlhF to B. cereus Pathogenicity Based on the observation that several virulence proteins...”
- Assessment of CcpA-mediated catabolite control of gene expression in Bacillus cereus ATCC 14579
van, BMC microbiology 2008 - “...5.56 4.38 1 mdh 3 Malate dehydrogenase BC4592 RZC01264 5.81 4.32 + citB Aconitate hydratase BC3616 RZC04993 1.62 1.26 1 odhA 1 Oxoglutarate dehydrogenase BC1252 RZC06639 2.07 3.80 4 odhB 2 Dihydrolipoyllysine-residue succinyltransferase BC1251 RZC03695 2.36 3.97 + sucC 1 Succinate-CoA ligase (ADP-forming) BC3834 RZC02282 8.23...”
ACNA_BRADU / P70920 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
bll0466 aconitase from Bradyrhizobium japonicum USDA 110
50% identity, 96% coverage
- function: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate (PubMed:8892815). Could catalyze the hydration of 2- methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnA functions as a RNA-binding regulatory protein (By similarity).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
catalytic activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis- aconitate + H2O (RHEA:17941)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer.
disruption phenotype: Cells lacking this gene grow more slowly, but also reach a lower cell density than the wild-type. The ability of the mutant to establish an effective root nodule symbiosis with soybean plants is not affected. - A bacterial iron exporter for maintenance of iron homeostasis
Sankari, The Journal of biological chemistry 2014 - “...transcripts were increased approximately 2-fold in the mutant, and bll0466 was 3-fold higher. blr4339 mRNA did not increase further in the mutant upon an iron...”
- “...The steady-state transcript levels of leuC, bfr, blr4339, bll0466, and blr0512 were analyzed by qualitative real-time PCR. The data are expressed as relative...”
- Genome-wide transcriptional and physiological responses of Bradyrhizobium japonicum to paraquat-mediated oxidative stress
Donati, Applied and environmental microbiology 2011 - “...tricarboxylic acid (TCA) cycle, such as aconitase (bll0466), enolase (bll4794), succinate dehydrogenase (blr0513), and most notably the pyruvate dehydrogenase...”
- The Iron control element, acting in positive and negative control of iron-regulated Bradyrhizobium japonicum genes, is a target for the Irr protein
Rudolph, Journal of bacteriology 2006 - “...California, Berkeley Increased expression under iron repletion bll5796 bll0466 blr2219 blr6742 blr0488 Gene name 742 RUDOLPH ET AL. A more global search for...”
DR1720 aconitate hydratase from Deinococcus radiodurans R1
Q9RTN7 Aconitate hydratase A from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
52% identity, 97% coverage
- Oxidative stress resistance in Deinococcus radiodurans
Slade, Microbiology and molecular biology reviews : MMBR 2011 - “...bypass Malate synthase (GlcB) Isocitrate lyase (AceA) DR0757 DR1720 DR1540 DR0287 DR0083 DR1247 DR1248 DR0700 DR0701 DR0698 DR0702 DR0697 DR0699 DR0695 DR0696...”
- Predicted highly expressed and putative alien genes of Deinococcus radiodurans and implications for resistance to ionizing radiation damage
Karlin, Proceedings of the National Academy of Sciences of the United States of America 2001 - “...divalent cation tolerance Tellurium resistance terD-2 sodC DR1720 DR1998 DR1275 DRA0259 DR2263 DR2221 DR1546 DR0791 DR1857 DR2294 DR2225 DRA0202 905 535...”
- “...DR2364 329 421 304 489 303 1.66 1.47 1.36 1.29 1.05 DR1720 DR0828 DR1540 DR0325 DR1247 DR0287 DR2627 DR1248 DR1674 DR0757 DR0083 905 463 430 329 385 955 812 300...”
- iTRAQ-based proteomic analysis of Deinococcus radiodurans in response to 12C6+ heavy ion irradiation
Gao, BMC microbiology 2022 - “...DNA repair. Group 5 includes proteins Q9RTU7 (Oligopeptidase A), Q9RUP1 (Glyceraldehyde-3-phosphate dehydrogenase), Q9RU54 (Isocitrate dehydrogenase), Q9RTN7 (Aconitate hydratase A), and Q9RR60 (Enolase), which are enzymes involved in either peptide or energy metabolism. The last one protein is Q9RWM2, an uncharacterized protein. The consistent upregulation of translation,...”
MA0250 aconitate hydratase from Methanosarcina acetivorans C2A
52% identity, 97% coverage
- Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis
Deere, BMC microbiology 2020 - “...protein to initially assess cluster transfer from IscU2. M. acetivorans encodes a single aconitase homolog (MA0250), and M. acetivorans cell lysate contains detectable aconitase activity (5.2nmol NADPH min 1 mg 1 protein) as measured by the coupled assay. Recombinant MA0250 was expressed in E. coli and...”
- “...A). As-purified MA0250 lacked aconitase activity. However, after chemical reconstitution with iron and sulfide, purified MA0250 exhibited robust aconitase activity (100nmol NADPH min 1 mg 1 protein) and a broad absorbance maximum around 400nm in UV-visible spectrum, consistent with the presence of a [4Fe-4S] cluster (Fig....”
B9SXB6 Aconitate hydratase from Ricinus communis
50% identity, 98% coverage
- Mapping the castor bean endosperm proteome revealed a metabolic interaction between plastid, mitochondria, and peroxisomes to optimize seedling growth
Wrobel, Frontiers in plant science 2023 - “...No. 4), peroxisomal isocitrate lyase (ICL, B9SUS2; Figure7 , No. 6) and cytosolic aconitase (ACO, B9SXB6; Figure7 , No. 5). Succinate produced by the glyoxylate cycle can enter the mitochondrial TCA cycle by the mitochondrial dicarboxylate/tricarboxylate carrier (DTC, B9T1E7, Picault etal., 2002 ; Toleco etal., 2020...”
- Proteomic Analysis of Embryo Isolated From Mature Jatropha curcas L. Seeds
Ramzan, Frontiers in plant science 2022 - “...Caleosin (Calcium ion binding protein) B9SQU8 AT4G26740.1 Jcr4s01018.60 3-Ketoacyl-CoA thiolase (KAT) B9RWL7 AT2G33150.1 Jcr4s09697.10 Aconitase B9SXB6 AT4G35830.1 Jcr4s00736.30 Aconitase B9T2U5 AT2G05710.1 Jcr4s00100.200 Malate synthase B9RAK0 AT5G03860.2 Jcr4S00086.110 Phospholipase D1/2 B9RV56 AT1G52570.1 Jcr4S01462.20 Chloroplastic oxoene reductase B9TA76 AT4G13010.1 Jcr4s00279.20 NAD-dependent malate dehydrogenase B9T5E4 AT5G43330.1 Jcr4s03576.70 NADP-dependent malic...”
- “...AT4G35090.1 Jcr4S00684.10 UTPglucose-1-phosphate uridylyltransferase B9SKS5 AT5G17310.2 Jcr4S00313.50 Glucan endo-1,3-beta-glucosidase B9T103 AT1G66250.1 Jcr4S09697.10 Aconitate hydratase (Aconitase) B9SXB6 AT4G35830.1 Jcr4S00736.30 Aconitate hydratase (Aconitase) B9T2U5 AT2G05710.1 Jcr4S00125.80 Succinyl-CoA synthetase B9RL91 AT5G23250.1 Jcr4s06387.30 Succinyl-CoA synthetase B9RL91 AT5G23250.1 Jcr4S03295.10 Malate dehydrogenase B9SE47 AT1G53240.1 Jcr4S00279.20 Malate dehydrogenase B9T5E4 AT5G43330.1 Jcr4s00055.90 Cytochrome C...”
- Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.)
Sharifi, Proteome science 2012 - “...42/51 5.8/6.18 GDP-D-mannose-3',5'-epimerase/ Malpighia glabra A0EJL8 15/108 6/3 1.4* 275 99/99 5.8/6.03 Aconitase/ Ricinus communis B9SXB6 7/85 6/2 9.1** 8.6** 1.0 1756 34/33 9.7/5.12 6-phosphogluconolactonase, putative/ Ricinus communis B9RWU6 12/86 2/1 -9.1** -7.7** 1.2 1443 35/43 4.9/4.91 Probable fructokinase-2/ Arabidopsis thaliana Q9LNE3 22/182 7/3 1.2 1199...”
XP_002530635 aconitate hydratase 1 from Ricinus communis
50% identity, 98% coverage
- The aconitate hydratase family from Citrus
Terol, BMC plant biology 2010 - “...Aco1 4 XM_002301587 XP_2301623 POPTRDRAFT_816803 Aco2 57 XM_002321090 XP_2321126 POPTRDRAFT_246575 Aco2 5 R. communis XM_002530589 XP_002530635 RCOM_0782740 Aco1 2 XM_002524138 XP_002524184 RCOM_0487910 Aco2 12 XM_002532518 XP_002532564 RCOM_0082520 Aco3 0 V. vinifera XM_002263301 XP_2263337 LOC100242027 Aco1 29 XM_002279224 XP_2279260 LOC100256776 Aco2 18 XM_002278102 XP_2278138 LOC100253811 Aco3 38...”
ACO3M_ARATH / Q9SIB9 Aconitate hydratase 3, mitochondrial; Aconitase 3; mACO1; Citrate hydro-lyase 3; EC 4.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
Q9SIB9 aconitate hydratase (EC 4.2.1.3) from Arabidopsis thaliana (see paper)
NP_178634 aconitase 3 from Arabidopsis thaliana
AT2G05710 aconitate hydratase, cytoplasmic, putative / citrate hydro-lyase/aconitase, putative from Arabidopsis thaliana
49% identity, 98% coverage
- function: Catalyzes the isomerization of citrate to isocitrate via cis- aconitate. Contributes to oxidative stress tolerance (PubMed:17013749). Modulates cytosolic citrate metabolism during lipid mobilization. Required during seedling growth (PubMed:25061985).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer (By similarity). Interacts with B'GAMMA in the cytosol (PubMed:25307043).
disruption phenotype: Reduced cytosolic and mitochondrial aconitase (ACO) activities by 25 and 55 precent, respectively (PubMed:17013749, PubMed:17437406). Increased tolerance to oxidative stress mediated by paraquat, a superoxide-generating agent (PubMed:17013749). Delayed early seedling growth, altered assimilation of acetate feeding and elevated citrate and malate levels (PubMed:25061985). - ACONITASE 3 is part of theANAC017 transcription factor-dependent mitochondrial dysfunction response.
Pascual, Plant physiology 2021 - GeneRIF: ACONITASE 3 is part of theANAC017 transcription factor-dependent mitochondrial dysfunction response.
- Protein phosphatase 2A (PP2A) regulatory subunit B'γ interacts with cytoplasmic ACONITASE 3 and modulates the abundance of AOX1A and AOX1D in Arabidopsis thaliana.
Konert, The New phytologist 2015 (PubMed)- GeneRIF: PP2A-B'gamma physically interacts with the cytoplasmic form of aconitase and PP2A-B'gamma impacts reactive oxygen species homeostasis by controlling the abundance of specific alternative oxidase isoforms, AOX1A and AOX1D, in leaf mitochondria.
- The aconitate hydratase family from Citrus
Terol, BMC plant biology 2010 - “...Acc. N Locus Aco class Associated ESTs A. thaliana NM_119749 NP_195308 At4g35830 Aco1 47 NM_126589 NP_178634 At2g05710 Aco2 183 NM_118831 NP_567763 At4g26970 Aco3 131 C. clementina FN552254 CBE71056 - Aco1 46 FN552256 CBE71058 - Aco2 93 FN552255 CBE71057 - Aco3 35 C. papaya DS981532 - -...”
- The iron-responsive element (IRE)/iron-regulatory protein 1 (IRP1)-cytosolic aconitase iron-regulatory switch does not operate in plants.
Arnaud, The Biochemical journal 2007 - GeneRIF: The identification of ACO1, ACO2, and ACO3 in A. thaliana is reported, and it was demonstrated that, in plants, the cytosolic ACOs are not converted into iron regulatory proteins.
- Evaluation of protein's interaction and the regulatory network of some drought-responsive genes in Canola under drought and re-watering conditions
Pasandideh, Physiology and molecular biology of plants : an international journal of functional plant biology 2023 - “...KASI Hsp81.4 HSP90.1 HSFA1E AT-HSFB2B HSP70 Q9LZC3 P28297 Q9SIB9 P57106 P25858 Q9SAJ4 F4I6W4 Q9ZW34 Q9FM97 Q9MAH0 Q9LMX8 Q8LEV7 Q9FY99 Q9SH69 Q9LD43 Q9LLC1...”
- Proteome Landscape during Ripening of Solid Endosperm from Two Different Coconut Cultivars Reveals Contrasting Carbohydrate and Fatty Acid Metabolic Pathway Modulation
Félix, International journal of molecular sciences 2023 - “...involved in the TCA cycle (variation V), i.e., the citrate synthase (P20115), citrate hydro-lyase (cis-aconitate-forming) (Q9SIB9), the cis-aconitate hydratase (Q9SIB9), succinate: quinone oxidoreductase (O82663), and malate dehydrogenase (Q9ZP06, mMDH1, and PMDH1), did not show changes in protein abundance during the maturation of the solid endosperm; the...”
- Proteome-wide analysis of hydrogen peroxide-induced protein carbonylation in Arabidopsis thaliana
Fangue-Yapseu, Frontiers in plant science 2022 - “...1180.69 1960.01 1384.79 2 2 3 2 21 F.Lyase activity AT2G05710 Aconitate hydratase 3 (ACO3) Q9SIB9 108.20 Mitochondrion (R)ILLESAIR(N) (K)TSLAPGSGVVTK(Y) (R)SNLVGMGIIPLCFK(S) (K)SGEDADTLGLTGHER(Y) 913.56 1115.62 1563.82 1556.71 2 2 2 2 6 AT5G11670 NADP-dependent malic enzyme 2 (NADP-ME2) Q9LYG3 64.41 Cytosol (R)DAHYLTGLLPPVILSQDVQER(K) (R)ILGLGDLGCQGMGIPVGK(L) (K)DLIGAVNAIKPTVLIGTSGVGQTFTK(E) (R)AIFGSGSPFDPVVYDGK(T) (K)TYDLGLASNLPR(A) (K)FAESSMYSPVYR(N)...”
- Protein Profiling of Arabidopsis Roots Treated With Humic Substances: Insights Into the Metabolic and Interactome Networks
Roomi, Frontiers in plant science 2018 - “...Q94C59 AT1G30690 Patellin-4 1.3 7.2 3 C, P, O80517 AT2G44790 Uclacyanin-2 1.3 30.2 5 P Q9SIB9 AT2G05710 Aconitate hydratase 2 1.3 17.7 9 CW, M, P, CH Q94A28 AT4G26970 Aconitate hydratase 3 1.3 17.9 14 M, CH O48773 AT2G32920 Protein disulfide-isomerase 2-3 1.3 10.2 4 ER...”
- iTRAQ-Based Quantitative Proteome Revealed Metabolic Changes in Winter Turnip Rape (Brassica rapa L.) under Cold Stress
Xu, International journal of molecular sciences 2018 - “...- - 0.366241266 Citrate cycle (TCA cycle) Q9SRZ6 Cytosolic isocitrate dehydrogenase [NADP] - 1.757858283 0.611169392 Q9SIB9 Aconitate hydratase 3 - - 0.659753955 O82662 Succinate-CoA ligase [ADP-forming] - - 0.633851199 Glyoxylate and dicarboxylate metabolism Q42624 Glutamine synthetase - - 0.36059385 Q9LQL0 Glycine cleavage system H protein 3...”
- Microsome-associated proteome modifications of Arabidopsis seedlings grown on board the International Space Station reveal the possible effect on plants of space stresses other than microgravity
Mazars, Plant signaling & behavior 2014 - “...AT2G20420 2.362 Succinyl-CoA ligase [GDP-forming] subunit O82663 AT5G66760 1.921 succinate dehydrogenase [ubiquinone] flavoprotein subunit 1 Q9SIB9 AT2G05710 1.718 aconitate hydratase 2 P20115 AT2G44350 1.694 citrate synthase 4 Sugar metabolism Q9C8Y9 AT1G66280 2.257 -glucosidase 22 Q9C525 AT1G66270 2.150 -glucosidase 21 Q940G5 AT4G25900 1.681 aldose 1-epimerase family protein...”
- A major role of class III HD-ZIPs in promoting sugar beet cyst nematode parasitism in Arabidopsis
Liu, PLoS pathogens 2024 - Decoding Arabidopsis thaliana CPK/SnRK Superfamily Kinase Client Signaling Networks Using Peptide Library and Mass Spectrometry
Ahsan, Plants (Basel, Switzerland) 2024 - “...the ABA-responsive element (ABRE) in their promoters [ 87 ]. Aconitase 3 ( Aco3 ; At2g05710) expression is upregulated by cytosolic Ca 2+ and contains ABRE in its promoter; it was found to be a client for both CPK1 and CPK17 in our screening. Aconitase 3...”
- Evaluation of protein's interaction and the regulatory network of some drought-responsive genes in Canola under drought and re-watering conditions
Pasandideh, Physiology and molecular biology of plants : an international journal of functional plant biology 2023 - “...70 kDa protein 4 AT5G03860 AT3G21720 AT2G05710 AT5G43330 AT3G04120 AT1G79550 AT1G70730 AT2G29560 AT5G56350 AT1G53310 AT1G13700 AT5G24420 AT5G13110 AT1G64190...”
- Genomically Hardwired Regulation of Gene Activity Orchestrates Cellular Iron Homeostasis in Arabidopsis
Hsieh, RNA biology 2022 - “...FBA8, At3g52930; iPGAM1, At1g09780; iPGAM2, At3g08590; ADH1, At1g77120; CSY4, At2g44350; CSY5, At3g60100; ACO2, At4g26970; ACO3, At2g05710; IAR4, At1g24180; MAB1, At5g50850; HOT5, At5g43940; ADH, At4g22110; ALDH2B7, At1g23800; ALDH2B4, At3g48000; ALDH3H1, At1g44170; PCK1, At4g37870; PPC1, At1g53310; IRT3, At1g60960; BGLU42, At5g36890; BTSL2, At1g18910; GRF11, At1g34760. Author contributions E.J.H. and...”
- Integrative transcriptomic and TMT-based proteomic analysis reveals the mechanism by which AtENO2 affects seed germination under salt stress
Wu, Frontiers in plant science 2022 - “...) (At2g36530), ENO1 (At1g74030), ENO3 (At2g29560), UBQ5 (At3g62250), AUX1 (AT2G38120), PIN4 (AT2G01420), AT3G22850 (AT3G22850), ACO3 (AT2G05710), HY5 (AT5G11260), LHCB3 (AT5G54270), PED1 (AT2G33150), GASA1 (AT1G75750), AOC2 (AT3G25770), GAPA1 (AT3G26650), GAPB (AT1G42970), APXT (AT1G77490), PIP1B (AT2G45960), CRY1 (AT4G08920), APX4 (AT4G09010), GGT1 (AT4G39640), PGK1 (At3g12780). Conclusion This is the...”
- Novel QTL for Low Seed Cadmium Accumulation in Soybean
Nissan, Plants (Basel, Switzerland) 2022 - “...genes (Aco1, -2, -3, -4, -5) and found Glyma.13G308700 clustered with the Arabidopsis Aco3 gene, At2g05710, and suggested that Glyma.13G308700 may be a paralog of another soybean Aco3 gene, Glyma.06G305700 , as a result of genetic duplication events. Glyma.13G308700 has a large number of GOs; in...”
- Proteome-wide analysis of hydrogen peroxide-induced protein carbonylation in Arabidopsis thaliana
Fangue-Yapseu, Frontiers in plant science 2022 - “...(R)SNSNFNAVILK(R) (R)APLGQNTVLLR(G) (K)HFGPAPGVPHSNTKPYVR(H) (K)IAVLVGTITDDLR(V) 1205.64 1180.69 1960.01 1384.79 2 2 3 2 21 F.Lyase activity AT2G05710 Aconitate hydratase 3 (ACO3) Q9SIB9 108.20 Mitochondrion (R)ILLESAIR(N) (K)TSLAPGSGVVTK(Y) (R)SNLVGMGIIPLCFK(S) (K)SGEDADTLGLTGHER(Y) 913.56 1115.62 1563.82 1556.71 2 2 2 2 6 AT5G11670 NADP-dependent malic enzyme 2 (NADP-ME2) Q9LYG3 64.41 Cytosol (R)DAHYLTGLLPPVILSQDVQER(K)...”
- Environmental Signals Act as a Driving Force for Metabolic and Defense Responses in the Antarctic Plant Colobanthus quitensis
Bertini, Plants (Basel, Switzerland) 2022 - “...AT4G22310), mitochondrial dihydrolipoyl dehydrogenase 1 (mtLPD1, AT1G48030), citrate synthase 4 (CSY4, AT2G44350), aconitase 3 (ACO3, AT2G05710), and fumarase 1 (FUM1, AT2G47510) ( Table S2 ). Downregulation of these proteins in the S3 vs. S2 data set implies their overexpression in plants growing in Site 2, indicating...”
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K7KYB1 Aconitate hydratase from Glycine max
50% identity, 99% coverage
Tfu_1925 aconitate hydratase 1 from Thermobifida fusca YX
Q47NL1 Aconitate hydratase from Thermobifida fusca (strain YX)
51% identity, 95% coverage
Smed_3083 aconitate hydratase from Sinorhizobium medicae WSM419
51% identity, 98% coverage
SMc03846 PROBABLE ACONITATE HYDRATASE PROTEIN from Sinorhizobium meliloti 1021
52% identity, 98% coverage
D7TEL2 Aconitate hydratase from Vitis vinifera
LOC100256776 aconitate hydratase, cytoplasmic from Vitis vinifera
50% identity, 99% coverage
- Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola
Liu, Horticulture research 2021 - “...0.7605 0.0235 F6I5U2 PDHX Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex 1.0167 0.8694 1.2357 0.0174 D7TEL2 ACO Aconitate hydratase 1.5400 0.0002 1.2926 0.0234 B6VJT4 SDH3 Succinate dehydrogenase subunit 3 1.0634 0.0046 1.3335 0.0009 D7SHR6 CA Carbonic anhydrase 1.8017 0.0003 0.9285 0.6448 D7TU30 CA Carbonic anhydrase 0.9075...”
- Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery
Liu, BMC plant biology 2014 - “...1.296 7.1.3 Vitis vinifera 6-phosphogluconate dehydrogenase A5BDU8 0.537 0.938 8.1.1.3 Vitis vinifera Dihydrolipoamide dehydrogenase, putative D7TEL2 0.672 1.64 8.1.3 Ricinus communis Aconitase F6HZK0 0.499 1 8.2.9 Vitis vinifera Malate dehydrogenase F6H9T6 0.466 0.972 8.2.99 Solanum lycopersicum Succinic semialdehyde dehydrogenase A5BQL5 0.628 0.795 8.3 Vitis vinifera Chloroplast...”
- The aconitate hydratase family from Citrus
Terol, BMC plant biology 2010 - “...12 XM_002532518 XP_002532564 RCOM_0082520 Aco3 0 V. vinifera XM_002263301 XP_2263337 LOC100242027 Aco1 29 XM_002279224 XP_2279260 LOC100256776 Aco2 18 XM_002278102 XP_2278138 LOC100253811 Aco3 38 G. max BT095399 Glyma01g36750 Aco1 435 AK286137 - Glyma11g08550 Aco1 61 - - Glyma06g46190 Aco3 38 AK244974 - Glyma12g10580 Aco3 25 AK286541 -...”
Q6YZX6 Putative aconitate hydratase, cytoplasmic from Oryza sativa subsp. japonica
50% identity, 99% coverage
- Proteomic Changes in Paspalum fasciculatum Leaves Exposed to Cd Stress
Salas-Moreno, Plants (Basel, Switzerland) 2022 - “...Cytoplasm 52 Q9XFA2 Phosphoenolpyruvate carboxykinase (ATP) 2 68.59 22.5239617 Urochloa panicoides 0.7 9.4 Cytoplasm 53 Q6YZX6 Putative aconitate hydratase 98.021 15.0334075 Oryza sativa subsp. japonica 1.5 6.4 Cytoplasm 54 Q6ZFT5 Ribose-phosphate pyrophosphokinase 4 36.126 17.8461538 Oryza sativa subsp. japonica 1.1 6.6 Cytosol, plasma membrane, cytoplasm, 55...”
- ITRAQ-based quantitative proteomic analysis of japonica rice seedling during cold stress
Qing, Breeding science 2022 - “...1.66 Q5VMJ3 Profilin LP04 6 58.78 0.70 Q7XKF3 Protochlorophyllide reductase A, chloroplastic 17 48.06 0.31 Q6YZX6 Putative aconitate hydratase, cytoplasmic 39 41.98 0.68 A0A0P0WK98 Ribosomal protein L15 (Fragment) 9 33.82 0.59 A3BLC3 Ribosome-recycling factor, chloroplastic 23 57.14 0.59 Q10M12 Ricin B-like lectin R40C1 15 53.45 0.55...”
- Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds
Zhang, Frontiers in plant science 2016 (no snippet) - Identification of differentially expressed proteins and phosphorylated proteins in rice seedlings in response to strigolactone treatment
Chen, PloS one 2014 - “...+ S5 Q8W317 Mitochondrial NADH:ubiquinone oxidoreductase (Complex I) 82148/5.9 46 54 296 1.7E-25 + S6 Q6YZX6 Cytosolic aconitate hydratase (ACO) 98591/5.7 44 43 314 2.6E-27 + S7 No identification 29.62.3 0.0032 S8 Q6VMN8 AGD2-like aminotransferase 50240/8.3 20 45 146 1.7E-10 - S9 No identification + T1...”
- Proteomic changes associated with expression of a gene (ipt) controlling cytokinin synthesis for improving heat tolerance in a perennial grass species
Xu, Journal of experimental botany 2010 - “...P49608 57 0.6 0.6 Putative aconitate hydratase (aconitase) [ Oryza sativa ] Cytoplasm 9883/5.67 342 Q6YZX6 70 1.5 2.1 2.1 Triosephosphate isomerase [H ordeum vulgare ] Cytoplasm 26720/5.39 270 gi|2507469 165 1.7 Glucose-6-phosphate isomerase (GPI) Cytoplasm 62237/6.96 195 P49105 192 0.4 NADH dehydrogenase [ Arabidopsis thaliana...”
ACO1 / Q42560 aconitase from Arabidopsis thaliana (see paper)
ACO1_ARATH / Q42560 Aconitate hydratase 1; Aconitase 1; Citrate hydro-lyase 1; EC 4.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
Q42560 aminocyclopropanecarboxylate oxidase (EC 1.14.17.4) from Arabidopsis thaliana (see paper)
NP_195308 aconitase 1 from Arabidopsis thaliana
AT4G35830, NP_195308 aconitate hydratase, cytoplasmic / citrate hydro-lyase / aconitase (ACO) from Arabidopsis thaliana
50% identity, 99% coverage
- function: Catalyzes the isomerization of citrate to isocitrate via cis- aconitate. Contributes to oxidative stress tolerance (PubMed:17013749). May have a role in respiration (PubMed:25061985).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer.
disruption phenotype: Reduced cytosolic and mitochondrial aconitase (ACO) activities by 70 and 20 precent, respectively (PubMed:17013749, PubMed:17437406). Increased tolerance to oxidative stress mediated by paraquat, a superoxide-generating agent (PubMed:17013749). Slightly higher production of CO(2) (PubMed:25061985). - H2 O2 , NO, and H2 S networks during root development and signalling under physiological and challenging environments: Beneficial or toxic?
Mukherjee, Plant, cell & environment 2023 - “...for brassinosteroid signalling Q9SCU7 Snitrosation Tavares et al. ( 2014 ) Primary metabolism Aconitate hydratase Q42560 Snitrosation Jain et al. ( 2018 ) Ssulfenylation Hu et al. ( 2015 ); Wei et al. ( 2020 ) Alcohol dehydrogenase 1 P14673 Snitrosation Dumont et al. ( 2018...”
- Cuscuta australis Parasitism-Induced Changes in the Proteome and Photosynthetic Parameters of Arabidopsis thaliana
Zagorchev, Plants (Basel, Switzerland) 2022 - “...response to cold 2107 130 O49344 Putative oxygen-evolving enhancer protein 2-2 Chloroplast photosynthesis 1301 212 Q42560 Aconitate hydratase 1 Mitochondrion Cytoplasm citrate metabolic process response to salt stress Stem 0407 269 O65396 Glycine cleavage T-protein family Mitochondrion glycine decarboxylation via glycine cleavage system response to cadmium...”
- Protein Profiling of Arabidopsis Roots Treated With Humic Substances: Insights Into the Metabolic and Interactome Networks
Roomi, Frontiers in plant science 2018 - “...V, ER, P, F4JWM1 AT5G18380 40S ribosomal protein S16-3 1.6 41.7 6 C, R, CH, Q42560 AT4G35830 Aconitate hydratase 1 1.5 19.9 9 EC Q9FVT2 AT1G57720 Probable elongation factor 1-gamma 2 1.5 14.3 7 CW, V Q9SMT7 AT3G48990 4-Coumarate-CoA ligase-like 10 1.5 21.0 12 EC, CH...”
- Comparative protein profiles of the Ambrosia plants.
Barton, Biochimica et biophysica acta. Proteins and proteomics 2017 - “...104136 5 100 35 18 1 1 1 ATP Synthesis Aconitate hydratase 1 A. thaliana Q42560 98153 4 100 18 20 1 1 1 Citrate Metabolism Adenosylhomocysteinase T. aestivum P32112 53438 2 100 10 18 1 1 One Carbon Metabolism Adenylate kinase A O. sativa.jap Q08479...”
- Plant-mPLoc: a top-down strategy to augment the power for predicting plant protein subcellular localization.
Chou, PloS one 2010 - “...Q9FV51, and Q9LJL3 can coexist in both chloroplast and mitochondrion while the protein with code Q42560 can coexist in both cytoplasm and mitochondrion, and the predicted results by Plant-mPLoc are exactly so. This is beyond the reach of TargetP [6] and Predotar [8] . From the...”
- The iron-responsive element (IRE)/iron-regulatory protein 1 (IRP1)-cytosolic aconitase iron-regulatory switch does not operate in plants.
Arnaud, The Biochemical journal 2007 - GeneRIF: The identification of ACO1, ACO2, and ACO3 in A. thaliana is reported, and it was demonstrated that, in plants, the cytosolic ACOs are not converted into iron regulatory proteins.
- Rewiring of primary metabolism for ammonium recycling under short-term low CO2 treatment - its implication for C4 evolution
Miao, Frontiers in plant science 2024 - “...of those involved in the tricarboxylic acid cycle (TCA) were markedly upregulated, specifically aconitase (ACO1, AT4G35830), isocitrate dehydrogenase 1 (IDH1, AT4G35260), 2-oxoglutarate dehydrogenase (OGDC, AT3G55410), succinate dehydrogenase (SDH, AT5G66760), and mMDH1 ( Figure3A ; Supplementary Dataset S1 ). However, we observed a significant reduction of the...”
- Assessing the Impacts of Cu and Mo Engineered Nanomaterials on Crop Plant Growth Using a Targeted Proteomics Approach
Li, ACS agricultural science & technology 2024 - “...redox P21 AT1G20620 catalase TWPEDVVPLQPVGR K TCA/org transformation P22 AT5G43330 malate dehydrogenase EFAPSIPEK P 23 AT4G35830 TCA aconitase VAEFSFR L tetrapyrrole biosynthesis P24 AT5G08280 tetrapyrrole synthesis porphobilinogen deaminase TLGELPAGSVIGSASLRR A Cu-based nanopesticide (Cu(OH) 2 -NMs) and Mo-based nanofertilizer (MoO 3 NMs) were selected as ENM treatments...”
- The Integration of Data from Different Long-Read Sequencing Platforms Enhances Proteoform Characterization in Arabidopsis
García-Campa, Plants (Basel, Switzerland) 2023 - “...FLOW 1 (HCEF1; AT3G54050), AMP-DEPENDENT SYNTHETASE AND LIGASE FAMILY PROTEIN (AA3; AT3G48990), ACONITASE 1 (ACO1; AT4G35830), CATALASE 3 (CAT3; AT1G20620) and CYTRATE SYNTHASE (CSY3; AT2G42790) ( Supplemental Table S6 ). In addition, 93 Iso-Seq proteoforms from 76 protein families, included in AtRTD3, were associated with leaf...”
- Genome-Wide Identification of the Aconitase Gene Family in Tomato (Solanum lycopersicum) and CRISPR-Based Functional Characterization of SlACO2 on Male-Sterility
Secgin, International journal of molecular sciences 2022 - “...4. Materials and Methods 4.1. Genome-Wide Identification and Analysis of SlACO Genes The AtACO1 sequence (AT4G35830) was obtained from the NCBI ( https://www.ncbi.nlm.nih.gov/gene/ , accessed on 10 October 2022) and used as a query for blast search against the tomato genome in the Phytozome v13 (...”
- Transition Zone1 Negatively Regulates Arabidopsis Aluminum Resistance Through Interaction With Aconitases
Liu, Frontiers in plant science 2021 - “...are available in TAIR 10 under the accession numbers TZ1 ( AT4G23870 ), ACO1 ( AT4G35830 ), ACO2 ( AT4G26970 ), and ACO3 ( AT2G05710 ). The Gene Expression Omnibus (GEO) accession number for the RNA-seq data reported in this paper is GSE189946 . Results Transition...”
- “...seeds. AT2G36120 DOT1 Encodes a glycine rich protein that is involved in leaf vascular patterning AT4G35830 ACO1 Encodes an aconitase that participates in the TCA cycle AT1G01010 NAC001 Response to hormone stimulus, lipid and oxygen-containing compound AT1G08780 PFD4 Negatively regulates cold acclimation AT5G28050 GSDA Involved in...”
- ACONITASE 3 is part of theANAC017 transcription factor-dependent mitochondrial dysfunction response
Pascual, Plant physiology 2021 - “...quantified with Protein Assay Dye Reagent (no. 5000006, Bio-Rad, Hercules, CA, USA). The individual ACO1 (AT4G35830), ACO2 (AT4G26970), and ACO3 (AT2G05710; www.arabidopsis.org ) isoforms were quantified by PRM. Samples consisting of 25g of protein were prepared for MS analysis as described in Trotta et al. (2019)...”
- “...from this article can be found in the EMBL/GenBank data libraries under accession numbers ACO1 (AT4G35830) , ACO2 (AT4G26970) , ACO3 (AT2G05710) , RBCL (ATCG00490) , RBCS1A (AT1G67090) , and GAPA (AT3G26650) . The Skyline files containing refined PRM and tSIM/PRM data as well as the...”
- Leaf Size Development Differences and Comparative Trancriptome Analyses of Two Poplar Genotypes
Zhang, Genes 2021 - “...7.99 10 37 Potri.015G004700 AT5G24510 60S acidic ribosomal protein family 3.6282 1.68 10 73 Potri.007G060900 AT4G35830 aconitase 1 9.7407 0 Potri.006G186800 AT2G24270 aldehyde dehydrogenase 11A3 8.8477 0 Potri.005G048100 AT3G04940 cysteine synthase D1 4.1698 8.44 10 33 Potri.004G054200 AT1G53240 lactate/malate dehydrogenase family protein 4.0327 9.24 10 49...”
- The function of glutaredoxin GRXS15 is required for lipoyl-dependent dehydrogenases in mitochondria
Moseler, Plant physiology 2021 - “...NR1 (At1g77760), NR2 (At1g37130), AAO1 (At5g20960), AAO2 (At3g43600), AAO3 (At2g27150), XDH1 (At4g34890) XDH2 (At4g34900), ACO1 (At4g35830), ACO2 (At4g26970), ACO3 (At2g05710), mLIP1 (At2g20860), IVDH (At3g45300), and BCAT (At5g65780). Supplemental data The following materials are available in the online version of this article. Supplemental Figure S1 . Arabidopsis...”
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CA51_26170, Mal33_51990 aconitate hydratase AcnA from Rosistilla oblonga
52% identity, 98% coverage
- Rosistilla oblonga gen. nov., sp. nov. and Rosistilla carotiformis sp. nov., isolated from biotic or abiotic surfaces in Northern Germany, Mallorca, Spain and California, USA
Waqqas, Antonie van Leeuwenhoek 2020 - “...Poly24_12210 TCA cycle Citrate synthase 2.3.3.16 gltA CA51_34870 Mal33_42290 Poly24_35470 Aconitate hydratase A 4.2.1.3 acnA CA51_26170 Mal33_51990 Poly24_20280 Isocitrate dehydrogenase [NADP] 1.1.1.42 icd CA51_38400 Mal33_38710 Poly24_37940 2-Oxoglutarate dehydrogenase E1 component 1.2.4.2 sucA CA51_08330 Mal33_06250 Poly24_05710 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2.3.1.61 sucB CA51_18830 Mal33_19360...”
- “...TCA cycle Citrate synthase 2.3.3.16 gltA CA51_34870 Mal33_42290 Poly24_35470 Aconitate hydratase A 4.2.1.3 acnA CA51_26170 Mal33_51990 Poly24_20280 Isocitrate dehydrogenase [NADP] 1.1.1.42 icd CA51_38400 Mal33_38710 Poly24_37940 2-Oxoglutarate dehydrogenase E1 component 1.2.4.2 sucA CA51_08330 Mal33_06250 Poly24_05710 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2.3.1.61 sucB CA51_18830 Mal33_19360 Poly24_28990...”
LOC100253811 aconitate hydratase, cytoplasmic from Vitis vinifera
50% identity, 97% coverage
- The aconitate hydratase family from Citrus
Terol, BMC plant biology 2010 - “...0 V. vinifera XM_002263301 XP_2263337 LOC100242027 Aco1 29 XM_002279224 XP_2279260 LOC100256776 Aco2 18 XM_002278102 XP_2278138 LOC100253811 Aco3 38 G. max BT095399 Glyma01g36750 Aco1 435 AK286137 - Glyma11g08550 Aco1 61 - - Glyma06g46190 Aco3 38 AK244974 - Glyma12g10580 Aco3 25 AK286541 - Glyma12g32000 Aco3 55 - -...”
LOC100242027 aconitate hydratase 1 from Vitis vinifera
49% identity, 99% coverage
- The aconitate hydratase family from Citrus
Terol, BMC plant biology 2010 - “...2 XM_002524138 XP_002524184 RCOM_0487910 Aco2 12 XM_002532518 XP_002532564 RCOM_0082520 Aco3 0 V. vinifera XM_002263301 XP_2263337 LOC100242027 Aco1 29 XM_002279224 XP_2279260 LOC100256776 Aco2 18 XM_002278102 XP_2278138 LOC100253811 Aco3 38 G. max BT095399 Glyma01g36750 Aco1 435 AK286137 - Glyma11g08550 Aco1 61 - - Glyma06g46190 Aco3 38 AK244974 -...”
F2DCZ4 Aconitate hydratase from Hordeum vulgare subsp. vulgare
50% identity, 97% coverage
D3GQL1 aconitate hydratase (EC 4.2.1.3) from Citrus clementina (see paper)
50% identity, 99% coverage
ACO2M_ARATH / Q94A28 Aconitate hydratase 2, mitochondrial; Aconitase 2; mACO2; Citrate hydro-lyase 2; EC 4.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
AT4G26970, NP_567763 aconitase 2 from Arabidopsis thaliana
NP_567763 aconitate hydratase/ copper ion binding from Arabidopsis thaliana
49% identity, 98% coverage
- function: Catalyzes the isomerization of citrate to isocitrate via cis- aconitate. Contributes to oxidative stress tolerance (PubMed:17013749). Involved in acetate assimilation (PubMed:25061985).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer.
disruption phenotype: Reduced mitochondrial aconitase (ACO) activity by 20 percent (PubMed:17013749, PubMed:17437406). Increased tolerance to oxidative stress mediated by paraquat, a superoxide-generating agent (PubMed:17013749). Altered acetate assimilation leading to lower levels of CO(2), CHO and SO, and higher OAs (organic acids) accumulation, especially fumarate (PubMed:25061985). - Integrative omics of Lonicera japonica Thunb. Flower development unravels molecular changes regulating secondary metabolites
Yang, Journal of proteomics 2019 - “...acetyl-CoA acetyltransferase, cytosolic 2 43,664 0.03 0.03 0.03 0.04 0.05 0.00000 Amino Acid meta. 21 Q94A28 Aconitate hydratase 3, mitochondrial 1E+05 0.03 0.03 0.03 0.04 0.05 0.01813 TCA cycle 22 Q38798 Calnexin homolog 2 60,737 0.02 0.02 0.02 0.03 0.04 0.00001 Signaling 23 P37142 Bifunctional aspartokinase/homoserine...”
- Protein Profiling of Arabidopsis Roots Treated With Humic Substances: Insights Into the Metabolic and Interactome Networks
Roomi, Frontiers in plant science 2018 - “...30.2 5 P Q9SIB9 AT2G05710 Aconitate hydratase 2 1.3 17.7 9 CW, M, P, CH Q94A28 AT4G26970 Aconitate hydratase 3 1.3 17.9 14 M, CH O48773 AT2G32920 Protein disulfide-isomerase 2-3 1.3 10.2 4 ER F4JMJ1 AT4G16660 Heat shock 70 kDa protein 17 1.3 4.3 3 ER...”
- Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station
Mazars, PloS one 2014 - “...uncharacterized protein At2g37970; T8P21.12) Tricarboxylic cycle P57106 AT5G43330 2.0606 Malate dehydrogenase, cytoplasmic 2 (EC 1.1.1.37) Q94A28 AT4G26970 1.7543 Aconitate hydratase 3, mitochondrial (Aconitase 3) (EC 4.2.1.3) (Citrate hydro-lyase 3) Hormone biosynthesis P38418 AT3G45140 2.8506 Lipoxygenase 2, chloroplastic (AtLOX2) (EC 1.13.11.12) Q9LS02 AT3G25770 2.961 Allene oxide cyclase...”
- Genomically Hardwired Regulation of Gene Activity Orchestrates Cellular Iron Homeostasis in Arabidopsis
Hsieh, RNA biology 2022 - “...PK, At3g49160; FBA8, At3g52930; iPGAM1, At1g09780; iPGAM2, At3g08590; ADH1, At1g77120; CSY4, At2g44350; CSY5, At3g60100; ACO2, At4g26970; ACO3, At2g05710; IAR4, At1g24180; MAB1, At5g50850; HOT5, At5g43940; ADH, At4g22110; ALDH2B7, At1g23800; ALDH2B4, At3g48000; ALDH3H1, At1g44170; PCK1, At4g37870; PPC1, At1g53310; IRT3, At1g60960; BGLU42, At5g36890; BTSL2, At1g18910; GRF11, At1g34760. Author contributions...”
- Integrative Analysis of Transcriptomic and Proteomic Changes Related to Cytoplasmic Male Sterility in Spring Stem Mustard (Brassica juncea var. tumida Tsen et Lee)
Shen, International journal of molecular sciences 2022 - “...synthase AT2G42790 DOWN DEG BjuA012893 K01681 aconitate hydratase - DOWN DEG BjuB043684 K01681 aconitate hydratase AT4G26970 DOWN DEG BjuA015174 K00417 ubiquinol-cytochrome c reductase subunit 7 AT5G25450 DOWN DEG novel.1027 K01535 H + transporting ATPase - DOWN DEG BjuA047355 K02133 F-type H + transporting ATPase subunit beta...”
- “...dehydrogenase AT2G18450 DOWN DEG BjuA026416 K00235 succinate dehydrogenase AT5G40650 DOWN DEG BjuB043684 K01681 aconitate hydratase AT4G26970 DOWN DEG BjuA012893 K01681 aconitate hydratase - DOWN DEG novel.1027 K01535 H + transporting ATPase - DOWN DEG BjuA047355 K02133 F-type H + transporting ATPase subunit beta - DOWN DEG...”
- ACONITASE 3 is part of theANAC017 transcription factor-dependent mitochondrial dysfunction response
Pascual, Plant physiology 2021 - “...Protein Assay Dye Reagent (no. 5000006, Bio-Rad, Hercules, CA, USA). The individual ACO1 (AT4G35830), ACO2 (AT4G26970), and ACO3 (AT2G05710; www.arabidopsis.org ) isoforms were quantified by PRM. Samples consisting of 25g of protein were prepared for MS analysis as described in Trotta et al. (2019) . The...”
- “...can be found in the EMBL/GenBank data libraries under accession numbers ACO1 (AT4G35830) , ACO2 (AT4G26970) , ACO3 (AT2G05710) , RBCL (ATCG00490) , RBCS1A (AT1G67090) , and GAPA (AT3G26650) . The Skyline files containing refined PRM and tSIM/PRM data as well as the DDA data generated...”
- Transition Zone1 Negatively Regulates Arabidopsis Aluminum Resistance Through Interaction With Aconitases
Liu, Frontiers in plant science 2021 - “...10 under the accession numbers TZ1 ( AT4G23870 ), ACO1 ( AT4G35830 ), ACO2 ( AT4G26970 ), and ACO3 ( AT2G05710 ). The Gene Expression Omnibus (GEO) accession number for the RNA-seq data reported in this paper is GSE189946 . Results Transition Zone1 Is Involved in...”
- The function of glutaredoxin GRXS15 is required for lipoyl-dependent dehydrogenases in mitochondria
Moseler, Plant physiology 2021 - “...NR2 (At1g37130), AAO1 (At5g20960), AAO2 (At3g43600), AAO3 (At2g27150), XDH1 (At4g34890) XDH2 (At4g34900), ACO1 (At4g35830), ACO2 (At4g26970), ACO3 (At2g05710), mLIP1 (At2g20860), IVDH (At3g45300), and BCAT (At5g65780). Supplemental data The following materials are available in the online version of this article. Supplemental Figure S1 . Arabidopsis mutants affected...”
- Magnetic Field Induced Changes in the Shoot and Root Proteome of Barley (Hordeum vulgare L.)
Shabrangy, Frontiers in plant science 2021 - “...Aconitate hydratase 0.9 0.01 27 236.78 Catalyzes the isomerization of citrate to isocitrate via cis-aconitate AT4G26970 ADD62592 A0A287R3A1 Clathrin heavy chain 0.5 0.002 39 323.31 Clathrin coat assembly, intracellular protein transport, vesicle-mediated transport AT3G08530 CDM83157 F2CWX3 Cytochrome P450 74a 2.22 0.002 22 90.98 Encodes a member...”
- Aconitase: To Be or not to Be Inside Plant Glyoxysomes, That Is the Question
De, Biology 2020 - “...maximum expression a gene reaches across all experiments); ACO1: aconitase 1 (At4g35830); ACO2: aconitase 2 (At4g26970); ACO3: aconitase 3 (At2g05710); ICL: isocitrate lyase (AT3G21720); MLS: malate synthase (At5g03860). ( B ) Data expressed as log2 scale; ACO1: aconitase 1 (At4g35830); ACO2: aconitase 2 (At4g26970); ACO3: aconitase...”
- “...localization of Arabidopsis aconitase protein by different available databases. Database/Program ACO1 * (At4g35830) ACO2 * (At4g26970) ACO3 * (At2g05710) The Plant Proteome Database ( https://ppdb.tc.cornell.edu ) mitochondria; cytosol mitochondria mitochondria; cytosol SUBcellular localisation database for Arabidopsis proteinsSUBA ( https://suba.plantenergy.uwa.edu.au ) mitochondria; cytosol mitochondria mitochondria Protein Localization...”
- Target of Rapamycin Regulates Genome Methylation Reprogramming to Control Plant Growth in Arabidopsis
Zhu, Frontiers in genetics 2020 - “...AT4G13890 0.5464 Hypo Exon SHM5| Pyridoxal phosphate-dependent transferase AT5G23250 0.6175 Hypo Exon/intron/utr3 ATP-citrate lyase/succinyl-CoA ligase AT4G26970 0.6402 Hypo Exon/intron ACO3| Aconitase/3-isopropylmalate dehydratase AT1G22020 0.6478 Hypo Exon/intron SHM6| Pyridoxal phosphate-dependent transferase AT5G11670 0.6696 Hypo Promoter NADP-ME2| Malic enzyme, NAD-binding AT1G79530 0.6699 Hypo Promoter GAPCP1| Glyceraldehyde 3-phosphate dehydrogenase...”
- “...0.5464 Hypo Exon SHM5| Pyridoxal phosphate-dependent transferase AT4G37670 0.5988 Hypo Exon/intron NAGS2| Acyl-CoA N -acyltransferase AT4G26970 0.6402 Hypo Exon/intron ACO3| Aconitase dehydratase large subunit AT1G22020 0.6478 Hypo Exon/intron SHM6| Pyridoxal phosphate-dependent transferase AT1G79530 0.6699 Hypo Promoter GAPCP1| Glyceraldehyde 3-phosphate dehydrogenase AT4G32840 1.5343 Hyper Promoter PFK6| Phosphofructokinase...”
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- Transcriptome analysis of Thapsia laciniata Rouy provides insights into terpenoid biosynthesis and diversity in Apiaceae
Drew, International journal of molecular sciences 2013 - “...TCA cycle enzyme Arabidopsis query Contig (identity) Reads Citrate synthase NP_850415 Comp7488 (84%) 12696 Aconitase NP_567763 Comp1618 (85%) 46037 Isocitrate dehydrogenase NP_175836 Comp214 (85%) 194838 2-oxoglutarate dehydrogenase NP_191101 Comp359 (85%) 162552 Dihydrolipoyllysine succinyltransferase NP_200318 Comp2028 (71%) 35422 Dihydrolipoyl dehydrogenase NP_567487 Comp12031 (84%) 7805 Succinyl-CoA synthetase NP_001119263...”
- The aconitate hydratase family from Citrus
Terol, BMC plant biology 2010 - “...Associated ESTs A. thaliana NM_119749 NP_195308 At4g35830 Aco1 47 NM_126589 NP_178634 At2g05710 Aco2 183 NM_118831 NP_567763 At4g26970 Aco3 131 C. clementina FN552254 CBE71056 - Aco1 46 FN552256 CBE71058 - Aco2 93 FN552255 CBE71057 - Aco3 35 C. papaya DS981532 - - Aco1 7 DS981607 - -...”
B9T2U5 Aconitate hydratase from Ricinus communis
51% identity, 96% coverage
- Mapping the castor bean endosperm proteome revealed a metabolic interaction between plastid, mitochondria, and peroxisomes to optimize seedling growth
Wrobel, Frontiers in plant science 2023 - “...TCA enzymes, with the exception of mitochondrial citrate synthase (mCSY), were present: mitochondrial aconitase (mACO, B9T2U5), mitochondrial NAD-dependent isocitrate dehydrogenase (mIDH, B9S0K1), 2-oxoglutarate dehydrogenase complex (ODC, B9SVA1 and B9SR46), the succinyl-CoA synthetase (SCoAL, B9SV11), succinate dehydrogenase complex (SDH, B9R7F6, B9SWW3, and B9SLS5), fumarase (FUM, B9SAW4), and...”
- Proteomic Analysis of Embryo Isolated From Mature Jatropha curcas L. Seeds
Ramzan, Frontiers in plant science 2022 - “...protein) B9SQU8 AT4G26740.1 Jcr4s01018.60 3-Ketoacyl-CoA thiolase (KAT) B9RWL7 AT2G33150.1 Jcr4s09697.10 Aconitase B9SXB6 AT4G35830.1 Jcr4s00736.30 Aconitase B9T2U5 AT2G05710.1 Jcr4s00100.200 Malate synthase B9RAK0 AT5G03860.2 Jcr4S00086.110 Phospholipase D1/2 B9RV56 AT1G52570.1 Jcr4S01462.20 Chloroplastic oxoene reductase B9TA76 AT4G13010.1 Jcr4s00279.20 NAD-dependent malate dehydrogenase B9T5E4 AT5G43330.1 Jcr4s03576.70 NADP-dependent malic enzyme B9RS07 AT5G25880.1 Jcr4s03356.50...”
- “...Jcr4S00313.50 Glucan endo-1,3-beta-glucosidase B9T103 AT1G66250.1 Jcr4S09697.10 Aconitate hydratase (Aconitase) B9SXB6 AT4G35830.1 Jcr4S00736.30 Aconitate hydratase (Aconitase) B9T2U5 AT2G05710.1 Jcr4S00125.80 Succinyl-CoA synthetase B9RL91 AT5G23250.1 Jcr4s06387.30 Succinyl-CoA synthetase B9RL91 AT5G23250.1 Jcr4S03295.10 Malate dehydrogenase B9SE47 AT1G53240.1 Jcr4S00279.20 Malate dehydrogenase B9T5E4 AT5G43330.1 Jcr4s00055.90 Cytochrome C oxidase, putative B9RV06 AT1G80230.1 Jcr4s01269.80 ATP...”
ABO_0694 aconitate hydratase from Alcanivorax borkumensis SK2
52% identity, 98% coverage
- An impaired metabolic response to hydrostatic pressure explains Alcanivorax borkumensis recorded distribution in the deep marine water column
Scoma, Scientific reports 2016 - “...II citrate synthase = 0.12 82.3 89.3 1152 ABO_1431 aconitate hydratase 1.27 321.6 778.2 872 ABO_0694 aconitate hydratase TCA cycle + 1.98 224.4 56.9 1871 ABO_1201 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase + 1.15 186.7 84.2 1441 ABO_0296 isocitrate dehydrogenase + 0.92 559.4 295.7 1344 ABO_1493 succinyl-CoA...”
- “...b556 subunit 0.76 162.4 275.5 128 ABO_0623 pyruvate dehydrogenase, E2 component 1.27 321.6 778.2 872 ABO_0694 aconitate hydratase ATP synthase + 3.38 1345.4 129.3 506 ABO_2730 atpF; ATP synthase F0 subunit B + 3.08 332.4 39.4 942 ABO_2729 atpH; ATP synthase subunit delta + 1.70 521.2...”
LOC107825601 aconitate hydratase, cytoplasmic from Nicotiana tabacum
50% identity, 98% coverage
PputW619_1631 aconitate hydratase from Pseudomonas putida W619
53% identity, 98% coverage
PP2112, PP_2112 aconitate hydratase 1 from Pseudomonas putida KT2440
52% identity, 98% coverage
- Computational analysis into the potential of azo dyes as a feedstock for actinorhodin biosynthesis in Pseudomonas putida
Nayyara, PloS one 2024 - “...ALDD2x, NTD11, FTHFD, PC, GARFT, ICL, IMPD, P5CD PP_4186, PP_4666, PP_4947, PP_2492, PP_4144, PP_2351, PP_2339, PP_2112, PP_2082, PP_1023, PP_0612, PP_3124, PP_0654, PP_2589, PP_0545, PP_2694, PP_1620, PP_0327, PP_1367, PP_1943, PP_1664, PP_5347, PP_4116, PP_1031, PP_4947 5 1 5 ALDD2y, FTHFD, ALDD2x, MDH, MICITDr, GLYOX, ACACT11, MALS, PGL, ACONTa,...”
- Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol
Beckers, Microbial cell factories 2016 - “...0.21 0.23 1.42 1.41 TCA cycle gltA PP4194 Citrate synthase 0.01 0.57 0.10 1.12 acnA PP2112 Aconitate hydratase 0.04 0.22 0.10 0.57 acnB PP2339 Aconitate hydratase 0.33 0.2 0.10 0.74 icd PP4012 Isocitrate dehydrogenase 0.38 0.14 0.10 1.97 sucA PP4189 2-Oxoglutarate dehydrogenase 0.37 0.08 0.10 0.17...”
- Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida
Borrero-de, Microbial cell factories 2014 - “...ppc PP1505 Phosphoenolpyruvate carboxylase 0.5 0.4 TCA cycle gltA PP4194 Citrate synthase 0.7 0.7 acnA PP2112 Aconitate hydratase 0.4 -0.4 acnB PP2339 Aconitate hydratase 0.7 0.3 icd PP4011 Isocitrate dehydrogenase 0.3 -0.5 sucA PP4189 2-Oxoglutarate dehydrogenase -0.7 -0.2 sucD PP4185 Succinyl-CoA synthetase sub alpha -0.1 -0.3...”
- “...ppc PP1505 Phosphoenolpyruvate carboxylase 0.0 1.4 TCA cycle gltA PP4194 Citrate synthase -0.2 0.7 acnA PP2112 Aconitate hydratase 0.8 -0.5 acnB PP2339 Aconitate hydratase -0.2 0.3 icd PP4011 Isocitrate dehydrogenase -0.3 -0.3 sucA PP4189 2-Oxoglutarate dehydrogenase -0.6 0.2 sucD PP4185 Succinyl-CoA synthetase sub alpha -0.2 0.5...”
- Differential proteomics and physiology of Pseudomonas putida KT2440 under filament-inducing conditions
Crabbé, BMC microbiology 2012 - “...and formation/downstream catabolism of urea (PP_0999, 0.23-fold; PP_1000, 0.28-fold; PP_1001, 0.24-fold) and glyoxylate (PP_4116, 0.27-fold; PP_2112, 0.42-fold and PP_4011, 0.25-fold), (iii) down-regulation of proteins involved in the production of ATP (PP_1478, 0.23-fold; PP_0126, 0.37-fold and PP_1478, 0.23-fold), (iv) differential expression of proteins involved in the metabolism...”
- “...OsmC family protein PP_2006 gi|26988731 0.12* Hypothetical protein PP_2006 PP_2105 gi|26988830 0.48 Hypothetical protein PP_2105 PP_2112 AcnA gi|26988836 0.42* Aconitate hydratase PP_2140 gi|26988864 0.47 Hypothetical protein PP_2140 PP_2303 HupB gi|26989027 0.52 Histone family protein DNA-binding protein PP_3089 gi|26989808 0.37* Hypothetical protein PP_3089 PP_3232 gi|26989950 0.16* Acetyltransferase...”
- Regulatory tasks of the phosphoenolpyruvate-phosphotransferase system of Pseudomonas putida in central carbon metabolism
Chavarría, mBio 2012 - “...dihydrolipoamide acetyltransferase (PP_0338); LpdG, dihydrolipoamide dehydrogenase (PP_4187); GltA, citrate synthase (PP_4194); AcnA, aconitate hydratase 1 (PP_2112); AcnB, aconitate hydratase 2 (PP_2339); Icd, isocitrate dehydrogenase (PP_4011); Kgd, alpha-ketoglutarate decarboxylase (PP_4189); KgdB, dihydrolipoamide acetyltransferase (PP_4188); SucA, 2-oxoglutarate dehydrogenase E1 component (PP_4189); SucC, succinyl-CoA synthetase beta subunit (PP_4186); SucD,...”
M8CZ57 Aconitate hydratase from Aegilops tauschii
50% identity, 98% coverage
- Physiological and Proteomic Analysis Responsive Mechanisms for Salt Stress in Oat.
Chen, Frontiers in plant science 2022 - “...2.033 I1I0Y4 Aconitase A ACO, acnA ns 4.221 M0VQ49 Aconitase A ACO, acnA ns 2.041 M8CZ57 Aconitase A ACO, acnA ns 2.046 W5FDW8 UDP-glucose 6-dehydrogenase UGDH, ugd ns 6.264 I1ISL8 UDP-N-acetylglucosamine pyrophosphorylase UGP2, galU, galF ns 2.337 Q43772 UDP-N-acetylglucosamine pyrophosphorylase UGP2, galU, galF ns 5.493 W5FGH0...”
Q1KSB0 Aconitate hydratase from Sorghum bicolor
49% identity, 98% coverage
ESA_01574 hypothetical protein from Enterobacter sakazakii ATCC BAA-894
51% identity, 99% coverage
JHW33_RS04170 aconitate hydratase AcnA from Rahnella aceris
50% identity, 97% coverage
CA54_47570 aconitate hydratase AcnA from Symmachiella macrocystis
52% identity, 95% coverage
XP_044405132 putative aconitate hydratase, cytoplasmic from Triticum aestivum
50% identity, 98% coverage
- 2D-DIGE based proteome analysis of wheat-Thinopyrum intermedium 7XL/7DS translocation line under drought stress
Lu, BMC genomics 2022 - “...dehydrogenase 1, mitochondrial KOG1335 58.81/7.63 100 2.65, 1.14, 2.14, 0, 0 0.019 Mito spot 32 XP_044405132 Putative aconitate hydratase KOG0452 93.9/5.66 100 5.24, 0.85, 8.77, 2.74, 0.94 0.026 Cyto spot 102 CAC14917 Triosephosphate-isomerase KOG1643 26.35/5.38 100 1.99, 2.2, 1.19, 0.28, 0.73 0.048 Cyto spot 139 EMS51931...”
LOC100304315 uncharacterized protein LOC100304315 from Zea mays
49% identity, 99% coverage
- Proteomics analysis reveals a dynamic diurnal pattern of photosynthesis-related pathways in maize leaves
Feng, PloS one 2017 - “...B6TQ36 pyruvate dehydrogenase E1 component subunit beta 7 5.05 2 396 42.29 7.02 C0HER4 uncharacterized LOC100304315 7 10.07 2 685 74.27 6.13 C0PFT6 uncharacterized LOC100383412 7 3.07 2 814 92.22 7.18 Carbon fixation in photosynthetic organisms Fifty identified proteins were enzymes involved in carbon fixation in...”
- The aconitate hydratase family from Citrus
Terol, BMC plant biology 2010 - “...distachyon - - Bradi1g75960 Aco1 3 - - Bradi3g15050 Aco4 40 Z. mays NM_001165757 NP_001159229 LOC100304315 Aco1 188 NM_001143012 NP_001136484 LOC100216599 Aco4 301 NM_001153959 NP_001147431 - Aco5 128 A multiple alignment with 48 Aco sequences was carried out, and a phylogenetic analysis was performed as described...”
acn / O53166 aconitase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
ACNA_MYCTU / O53166 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
Rv1475c aconitate hydratase from Mycobacterium tuberculosis H37Rv
NP_215991 iron-regulated aconitate hydratase from Mycobacterium tuberculosis H37Rv
Mb1511c PROBABLE ACONITATE HYDRATASE ACN (Citrate hydro-lyase) (Aconitase) from Mycobacterium bovis AF2122/97
50% identity, 97% coverage
- function: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. The apo form of AcnA functions as a RNA-binding regulatory protein which binds to selected IRE-like sequences present within the UTRs (untranslated regions) of 3' trxC and 5' IdeR mRNA (PubMed:17384188). Could catalyze the hydration of 2-methyl-cis- aconitate to yield (2R,3S)-2-methylisocitrate (By similarity).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
catalytic activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis- aconitate + H2O (RHEA:17941)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer. - Aptamer-Based Diagnostic Systems for the Rapid Screening of TB at the Point-of-Care
Martin, Diagnostics (Basel, Switzerland) 2021 - “...samples from active TB patients. Protein Name Accession # M. tb Specific Reference Aconitate hydratase Rv1475c X [ 186 ] Conserved protein Rv1977 + X [ 186 ] Serine/threonine protein kinase Rv0014c [ 186 ] DNA translocase FtsK Rv2748c [ 186 ] Nitrate reductase -subunit Rv1161...”
- One-Year Old Dormant, "Non-culturable" Mycobacterium tuberculosis Preserves Significantly Diverse Protein Profile
Trutneva, Frontiers in cellular and infection microbiology 2020 - “...alpha-antigen) Rv1094 Possible acyl-[acyl-carrier protein] desaturase DesA2 (acyl-[ACP] desaturase) (stearoyl-ACP desaturase) Rv1023 Probable enolase Eno Rv1475c Probable iron-regulated aconitate hydratase Acn (citrate hydro-lyase) (aconitase) Rv0815c Probable thiosulfate sulfurtransferase CysA2 Rv3246c Two component sensory transduction transcriptional regulatory protein MtrA Rv1436 Probable glyceraldehyde 3-phosphate dehydrogenase Gap (GAPDH) Rv3224...”
- Resuscitation of Dormant "Non-culturable" Mycobacterium tuberculosis Is Characterized by Immediate Transcriptional Burst
Salina, Frontiers in cellular and infection microbiology 2019 - “...of enzymes belonging to the tricarboxylic acid cycle: sdhAB (Rv3318, Rv3319, succinate dehydrogenase subunit), acn (Rv1475c, aconitase) , sucCD (Rv0951, Rv0952, succinyl-CoA synthetase subunits), gltA2 (Rv0896, citrate synthase I), ndkA (Rv2245c, nucleoside phosphate kinase participating in nucleoside biosynthesis), and pntAb and pntB (Rv0156, Rv0157, transhydrogenase subunits...”
- Structural and functional evidence that lipoprotein LpqN supports cell envelope biogenesis in Mycobacterium tuberculosis
Melly, The Journal of biological chemistry 2019 (secret) - ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes
Murphy, mBio 2018 - “...Rv1005c pabB Probable para-aminobenzoate synthase component Rv1093 glyA1 Serine hydroxymethyltransferase Rv1392 metK Probable S-adenosylmethionine synthetase Rv1475c acn Probable iron-regulated aconitate hydratase Rv1743 pknE Probable transmembrane serine/threonine protein kinase E Rv2241 aceE Pyruvate dehydrogenase E1 component Rv2398c cysW Probable sulfate transport membrane protein ABC transporter Rv2746c pgsA3...”
- Mycobacterium tuberculosis Rv1474c is a TetR-like transcriptional repressor that regulates aconitase, an essential enzyme and RNA-binding protein, in an iron-responsive manner
Balakrishnan, Tuberculosis (Edinburgh, Scotland) 2017 (PubMed)- “...tetracycline binding protein is operonic with aconitase (Rv1475c) in H37Rv</ce:bold>. (A) Schematic representation of the genomic organization of <ce:italic>acn...”
- “...to the inverted repeats in the <ce:italic>acn</ce:italic> (Rv1475c) upstream region. (A) Schematic representation and the sequence of 220 bp...”
- Commentary: Modification of Host Responses by Mycobacteria
Kumar, Frontiers in immunology 2017 - “...immune system within host and provide an unfavorable environment for M.tb to survive. M.tb ORF Rv1475c encoded aconitase is an iron binding protein that has conserved residues of the iron-responsive class of proteins and binds to iron-responsive elements in case of iron depletion ( 29 )....”
- Comparing isogenic strains of Beijing genotype Mycobacterium tuberculosis after acquisition of Isoniazid resistance: A proteomics approach
Nieto, Proteomics 2016 - “...factor Rho rho Rv1297 IP 1.9 Thioredoxin Rv1324 Rv1324 IMR 3.2 Ironregulated aconitate hydratase acn Rv1475c IMR 1.3 Glycine dehydrogenase gcvB Rv1832 IMR 3.6 Catalaseperoxidaseperoxynitritase T KatG katG Rv1908c V 2.6 Monophosphatase cysQ Rv2131c IMR 7.5 Pyruvate dehydrogenase E1 component aceE Rv2241 IMR 1.2 Conserved protein...”
- More
- Iron-dependent RNA-binding activity of Mycobacterium tuberculosis aconitase.
Banerjee, Journal of bacteriology 2007 - GeneRIF: Results demonstrate the bifunctional nature of Acn, pointing to its likely role in iron homeostasis.
- A web-based protein interaction network visualizer
Salazar, BMC bioinformatics 2014 - “...Network, Circle and Table. Figure 2 -B displays a snapshot of the interactions for proteins O53166 and O05839 from the MTB dataset in the Network layout. The network layout allows the re-organization of the proteins through drag and drop mouse gestures, and clicking on a node...”
- Genome-scale identification and characterization of moonlighting proteins
Khan, Biology direct 2014 - “...17 in MPR1 (Figure 2 A). This protein is aconitase of Mycobacterium tuberculosis (UniProt ID: O53166), which has TCA cycle enzyme as F1 and iron-responsive protein as F2. This protein switches between the two functions depending on the cellular iron levels, namely, binding of a 4Fe-4S...”
- Fumarate reductase activity maintains an energized membrane in anaerobic Mycobacterium tuberculosis
Watanabe, PLoS pathogens 2011 - “...Rv0249c: O53671; mez: P71880; pca: P95127; pckA: P65686; tgs: P0A650; hspX: P0A5B7; citA: P63777; acn: O53166; kgd: O50463 Supporting Information Figure S1 Transcriptional profile of genes related to central carbon metabolism under various dissolved oxygen tensions. Gene expression levels of 8, 2, 1, 0.5 and 0.1...”
- Identification of B cell antigenome in Mycobacterium bovis by immunoproteomic analysis
Cho, Acta veterinaria Hungarica 2020 (PubMed)- “...cell antigens, including 13 new proteins, i.e. Mb3751, Mb2006c, Mb3276c, Mb2244, Mb1164c, Mb2553c, Mb2946c, Mb1849c, Mb1511c, Mb1034c, Mb2616c, Mb0854c and Mb2267. These new proteins identified by 2-DE and immunoblotting were the B cell antigens used in developing serological diagnostic methods based on HI to bTB....”
SAMCCGM7_Ch3507 aconitate hydratase AcnA from Sinorhizobium americanum CCGM7
51% identity, 98% coverage
PFL_1929 aconitate hydratase 1 from Pseudomonas fluorescens Pf-5
Q4KFD5 Aconitate hydratase from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
52% identity, 98% coverage
PSHAa0159 aconitate hydratase (likely to be used in stationary phase); likely to have a 4Fe-4S cluster from Pseudoalteromonas haloplanktis TAC125
51% identity, 97% coverage
MAP1201c aconitate hydratase from Mycobacterium avium subsp. paratuberculosis str. k10
50% identity, 98% coverage
- Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays
Li, PloS one 2017 - “...cloned into the pMAL-c5 translational fusion expression vector (New England Biolabs, Beverly, MA, USA). The MAP1201c + 2942c was chemically synthesized, amplified and cloned in a manner similar to the other 5 genes. The vector and amplification products were each digested with Xba I and Hind...”
- “...of serum and milk MFI values (Mann-Whitney test). Sample Type MAP1272c MAP1569 MAP2121c MAP2942c MAP2609 MAP1201c + 2942c Serum, n = 180 NL (MSD) 1217.8 836.3 891.0 1336.1 740.2 2129.4 1327.7 705.2 723.4 1047.5 455.2 1906.1 F+E- (MSD) 2086.2 1410.0 1246.9 2086.9 1207.2 3257.7 3674.0 1353.2...”
- Iron-sparing response of Mycobacterium avium subsp. paratuberculosis is strain dependent
Janagama, BMC microbiology 2010 - “...expression pattern. Selected genes included siderophore transport (MAP2413c, MAP2414c), esx-3 secretion system (MAP3783, MAP3784), aconitase (MAP1201c), fatty acid metabolism (MAP0150c) and virulence (MAP0216, MAP3531c, MAP1122 and MAP0475). RNA was treated with DNaseI (Ambion, Austin, TX) and one step Q-RT PCR was performed using QuantiFast SYBR Green...”
- “...Interestingly, during iron limitation, the cattle strain but not sheep MAP downregulated expression of aconitase (MAP1201c) and succinate dehydrogenases (MAP3697c, MAP3698c) (Figure 2 ). Repression of aconitase in response to iron starvation is post-transcriptionally mediated via small RNAs [ 36 ]. Consistent with this finding, our...”
G7JYQ8 Aconitate hydratase from Medicago truncatula
50% identity, 99% coverage
M1CTR3 Aconitate hydratase from Solanum tuberosum
49% identity, 98% coverage
Q10S34 Aconitate hydratase from Oryza sativa subsp. japonica
49% identity, 99% coverage
- Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds.
Yan, International journal of molecular sciences 2021 - “...to fumarate and transfers electrons to ubiquinone [ 45 ]. In the present study, ACO (Q10S34) was up-accumulated in T12 and T36 seeds, but SDHA (F2E611) was down-accumulated in T12 seeds, with no significant changes in T24 or T36 seeds, revealing that the energy supply for...”
- “...D-lactate dehydrogenase [cytochrome], mitochondrial (Fragment) 0.234 0.0023 0.535 0.0096 0.618 0.0294 Citrate cycle (TCA cycle) Q10S34 Aconitate hydratase 1.680 0.0076 1.206 0.1089 1.602 0.0193 F2E611 Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial 0.520 0.0072 0.818 0.0199 1.004 0.9945 Amino acid metabolism Phenylalanine, tyrosine and tryptophan biosynthesis A0A1D6RN38...”
Pan258_21500 aconitate hydratase AcnA from Symmachiella dynata
52% identity, 95% coverage
MAV_3303 aconitate hydratase from Mycobacterium avium 104
50% identity, 98% coverage
- Surface proteome of "Mycobacterium avium subsp. hominissuis" during the early stages of macrophage infection
McNamara, Infection and immunity 2012 - “...RPOB_MYCA1 Extracellular DMEM Intracellular 24 48 24 48 24 48 24 48 MAV_0182 MAV_4707 MAV_3303 MAV_3341 4 18 9 7 4 11 5 7 5 19 7 8 4 17 5 8 3 7 9 6 2 10 8 9...”
- “...MAV_3813 MAV_5146 MAV_1525 MAV_1527 MAV_1526 MAV_1524 MAV_3303 MAV_3615 MAV_1262 MAV_4916 MAV_2753 MAV_2267 MAV_1075 MAV_3137 MAV_3850 MAV_4365 MAV_4451...”
- Adaptive Changes in Mycobacterium avium Gene Expression Profile Following Infection of Genetically Susceptible and Resistant Mice
Ignatov, Acta naturae 2010 - “...protein MAV_2926 PPE protein MAV_2244 GlnA; glutamine synthetase MAV_4011 NO-reductase, ?-subunit MAV_1074 SucC; succinyl-CoA-synthase, ?-subunit MAV_3303 AcnA; aconitate hydratase MAV_1130 NADH-dehydrogenase I, H-subunit MAV_4040 NADH-dehydrogenase I, H-subunit MAV_1524 ATP-synthase F 1 F O , ?-subunit MAV_5034 Transposase MAV_1059 Transposase Qualitative (determination of nucleotide sequences of specifically...”
- “...more active and diverse expression of genes coding for the Krebs cycle enzymes: MAV_1074 and MAV_3303 coding for succinyl-Csynthesase and aconitate hydratase, respectively; and of genes coding for the proteins important for oxidative phosphorylation, as well as the respiratory electron-transport chain proteins: MAV_1130 , MAV_4040 ,...”
D3GQL0 aconitate hydratase (EC 4.2.1.3) from Citrus clementina (see paper)
50% identity, 98% coverage
Mal52_21950 aconitate hydratase AcnA from Symmachiella dynata
51% identity, 95% coverage
FZF21_20425 aconitate hydratase AcnA from Enterobacter sp. LU1
51% identity, 99% coverage
LT988_17115 aconitate hydratase AcnA from Thiocapsa bogorovii
51% identity, 98% coverage
D3GQL2 aconitate hydratase (EC 4.2.1.3) from Citrus clementina (see paper)
50% identity, 99% coverage
acnA / Q8ZP52 aconitate hydratase 1 (EC 4.2.1.99) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
ACNA_SALTY / Q8ZP52 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; 2-methyl-cis-aconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
STM1712 aconitate hydratase 1 from Salmonella typhimurium LT2
NP_460671 aconitate hydratase 1 from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
51% identity, 99% coverage
- function: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and the 2- methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Also catalyzes the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The (2S,3S)-2-methylcitrate (2-MC) is a very poor substrate (PubMed:11294638). The apo form of AcnA functions as a RNA-binding regulatory protein (By similarity).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
catalytic activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis- aconitate + H2O (RHEA:17941)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer.
disruption phenotype: Cells lacking this gene show no phenotype during growth on propionate, acetate, succinate, glycerol and glucose. The acnAB double mutant does not grow on propionate even when supplemented with glutamate and is unable to respire propionate under anaerobic growth conditions. - Transcriptomic Responses of Salmonella enterica Serovars Enteritidis in Sodium Hypochlorite
Wang, Frontiers in cellular and infection microbiology 2022 - “...malate dehydrogenase STM0112 leuB -1.8 3-isopropylmalate dehydrogenase STM0158 acnB -2.6 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase STM1712 acnA -1.2 aconitate hydratase AcnA STM0965 dmsB -1.8 dimethylsulfoxide reductase subunit B Oxidative stress response STM0609 ahpF 1.5 Alkyl hydroperoxide reductase protein F, partial STM4266 soxR 1.2 redox-sensitive transcriptional activator...”
- Repression of Salmonella enterica phoP expression by small molecules from physiological bile
Antunes, Journal of bacteriology 2012 - “...STM1231 STM4159 STM3557 STM0734 STM0662 ORF01131 STM1238 STM1712 STM0549 STM2141 STM0735 STM0738 STM1744 PSLT046 STM2190 STM2799 STM1292 STM0730 STM3321 STM1742...”
- Characterization of Salmonella enterica serovar Typhimurium aconitase A.
Baothman, Microbiology (Reading, England) 2013 (PubMed)- GeneRIF: Promoter fusion experiments indicated that acnB transcription was ~10-fold greater than that of acnA.
P792_14035 aconitate hydratase AcnA from Asaia sp. SF2.1
50% identity, 97% coverage
- Blood meal-induced inhibition of vector-borne disease by transgenic microbiota
Shane, Nature communications 2018 - “...Bfr Escherichia coli Iron storage P792_12870 74 68 AGLR1.AcnA AcnA Escherichia coli TCA cycle enzyme P792_14035 76 69 a AGLR1.HF was constructed using the same but greatly shortened promoter region used in AGLR1.Hem Six of eight putative BMI promoters exhibited conditional expression when the bacteria were...”
Q8Z7D2 Aconitate hydratase from Salmonella typhi
t1625 aconitate hydratase 1 from Salmonella enterica subsp. enterica serovar Typhi Ty2
51% identity, 99% coverage
UH47_06710 aconitate hydratase AcnA from Staphylococcus pseudintermedius
51% identity, 99% coverage
GSU0846 aconitate hydratase 1 from Geobacter sulfurreducens PCA
53% identity, 97% coverage
Oant_0105 aconitate hydratase 1 from Ochrobactrum anthropi ATCC 49188
52% identity, 97% coverage
- A novel A3 group aconitase tolerates oxidation and nitric oxide
Doi, The Journal of biological chemistry 2015 - “...YD35 E. coli DH10B P. aeruginosa PAO1 R. eutropha NBRC 102504 Oant_0105 SJA_C1-23180 36 3 30 1 33 2 26 1 AcnA3 BB1844 Daci_2441 H16_A2638 107 3 82 3 73 9 74...”
- “...AcnA isozymes from Ochrobactrum anthropi (Oant_0105), Sphingobium japonicum (SJA_C1-23180), Bordetella bronchiseptica (BB1844), Delftia acidovorans (Daci_2441),...”
BS1330_I0093 aconitate hydratase AcnA from Brucella suis 1330
51% identity, 98% coverage
Mmah_0478 aconitase from Methanohalophilus mahii DSM 5219
50% identity, 97% coverage
RLV_6777 aconitate hydratase AcnA from Rhizobium leguminosarum bv. viciae
50% identity, 98% coverage
ACIAD3090 aconitate hydratase 1 from Acinetobacter sp. ADP1
51% identity, 98% coverage
BAB1_0090 Aconitate hydratase, C-terminal:Aconitate hydratase, N-terminal:Aconitate hydratase 1 from Brucella melitensis biovar Abortus 2308
51% identity, 98% coverage
- Iron-dependent reconfiguration of the proteome underlies the intracellular lifestyle of Brucella abortus
Roset, Scientific reports 2017 - “...F0F1 ATP synthase subunit delta C NO 0.59 0.15 Tricarboxylic acid (TCA) cycle GI: 82699012 BAB1_0090 AcnA aconitate hydratase C NO 0.54 0.11 GI: 82616088 BAB1_1170 gltA citrate synthase C NO 1.38 0.12 GI: 82700033 BAB1_1221 isocitrate dehydrogenase C NO 1.31 0.13 GI: 82616792 BAB1_1926 sucC...”
- “...iron for their activity 28 . Accordingly, we found in this study, that Brucella aconitase (BAB1_0090), one of the critical enzymes from the TCA cycle, was severely down-regulated within the host cell (Table 2 and Fig. 4 ). Similar findings were reported in the bacterium Staphylococcus...”
- Intracellular adaptation of Brucella abortus
Lamontagne, Journal of proteome research 2009 - “...metabolism were down regulated. For instance, tricarboxylic acid (TCA) cycle components, such as aconitate hydratase (BAB1_0090), 2-oxoglutarate dehydrogenase (BAB1_1923), dihydrolipoamide acetyltransferase (BAB1_1922), succinyl-CoA synthetase (BAB1_1925) and malate dehydrogenase (BAB1_1927), were all reduced at similar rates 3 hours after infection. Similarly, transketolase (BAB1_1740) and enolase (BAB1_1155), enzymes...”
- “...carbon metab GalE 6 BAB1_1149 Central carbon metab LpdA-1 6 BAB1_1666 Protein synthesis RluD 7 BAB1_0090 Central carbon metab AcnA 7 BAB1_1150 Central carbon metab Ace 7 BAB2_0631 Protein synthesis LSU L33 8 BAB1_0246 Central carbon metab Gdh 8 BAB2_0938 Central carbon metab Xyl 8 BAB2_0635...”
BMEI1855 ACONITATE HYDRATASE from Brucella melitensis 16M
51% identity, 98% coverage
- Integrated Bioinformatics-Based Subtractive Genomics Approach to Decipher the Therapeutic Drug Target and Its Possible Intervention against Brucellosis
Khan, Bioengineering (Basel, Switzerland) 2022 - “...other proteins nearby like: malate synthase G, involved in the glycolate utilization (glcB); aconitate hydratase (BMEI1855), (BMEII0009), (BMEI1952), (BMEII1061), (BMEI1939), (BMEII1060), (BMEII1062), (BMEII1064); and ureidoglycolate lyase (allA) and (BMEI0799). The PPI results showed that isocitrate lyase had a total number of nodes 11, average node numbers...”
- RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress
Liu, Scientific reports 2015 - “...BMEI0138; BMEI0139; sucA, BMEI0140; BMEI0791; gltA, BMEI0836; BMEI0855; BMEI0856; class I fumarate hydratase, BMEI1016; acn, BMEI1855; fumC, BMEII1051; and citrate lyase beta chain, BMEII1074; Fig. 2 ). The TCA cycle is critical for carbon metabolism and energy generation. The pyruvate metabolism supplies energy to living cells...”
- “..., protoheme ix farnesyltransferase 0.014 BMEI1652 Urease alpha subunit 0.022 BMEI1758 blxR, transcriptional activator 0.043 BMEI1855 aconitate hydratase 0.014 BMEII0025 virb 1, type IV secretion system 0.011 BMEII0035 virb 11, type IV secretion system 0.026 BMEII0071 gntR , transcriptional regulator 0.011 BMEII0245 usp , universal stress...”
- Comparative proteome analysis of Brucella melitensis vaccine strain Rev 1 and a virulent strain, 16M
Eschenbrenner, Journal of bacteriology 2002 - “...4.88 4.94 42.1 36.1 5.0-6.0 5 6 7 Aconitate hydratase ND EF-Tud BMEI1855 5.52 5.51 5.34 5.55 109.1 51.6 44.9 97.9 8 ND 9 10 11 12 ND ND ND ND 6.09 5.47 4.73...”
lpl1653 Aconitate hydratase from Legionella pneumophila str. Lens
50% identity, 98% coverage
ACON_LEGPH / P37032 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; IP210; Iron-responsive protein-like; IRP-like; Major iron-containing protein; MICP; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) (see paper)
lpg1690 aconitate hydratase from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
51% identity, 98% coverage
- function: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis- aconitate. The apo form of AcnA functions as a RNA-binding regulatory protein (PubMed:8366052). Could catalyze the hydration of 2-methyl-cis- aconitate to yield (2R,3S)-2-methylisocitrate (By similarity).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
catalytic activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis- aconitate + H2O (RHEA:17941)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer. - Role of Legionella pneumophila outer membrane vesicles in host-pathogen interaction
Ayesha, Frontiers in microbiology 2023 - “...metabolism; peptidoglycan biosynthesis No Nucleoside diphosphate kinase (ndk) lpg1548 Nucleotide metabolism No Aconitate hydratase (acnA) lpg1690 Carbohydrate metabolism; energy metabolism Mycobacterium tuberculosis ( Lee et al., 2015 ) Bacteroides fragilis ( Zakharzhevskaya et al., 2017 ) Long-chain fatty acid transporter lpg1810 Lipid metabolism No S-adenosylmethionine synthetase...”
- Legionella pneumophila type II secretome reveals unique exoproteins and a chitinase that promotes bacterial persistence in the lung
DebRoy, Proceedings of the National Academy of Sciences of the United States of America 2006 - “...lpp1018 lpp2697 lpl0985 lpl2569 Y Y lpg0873 lpg1690 lpg2025 lpg1340 lpg2622 lpp0936 lpp1659 lpp2007 lpp1294 lpp1177 lpp2675 lpl0906 lpl1653 lpl2002 lpl1293...”
BQ01090 Aconitate hydratase from Bartonella quintana str. Toulouse
50% identity, 97% coverage
SACE_3811 aconitate hydratase from Saccharopolyspora erythraea NRRL 2338
50% identity, 95% coverage
- Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production
Ke, Applied microbiology and biotechnology 2022 (PubMed)- “...We found out that the activity of aconitase (SACE_3811) was significantly inhibited by succinylation in E3sucC, which probably led to the extracellular...”
- “...Cloning, expression, purification, and reactivated of Acn (SACE_3811) Cells samples were harvested by centrifugation and resuspended in PBS buffer containing...”
- Systems perspectives on erythromycin biosynthesis by comparative genomic and transcriptomic analyses of S. erythraea E3 and NRRL23338 strains
Li, BMC genomics 2013 - “...E3. Most genes of TCA cycle, including SACE_1638 ( sucB ), SACE_3674 ( mdh ), SACE_3811 ( acn ), SACE_3926/SACE_3927 ( korA ), SACE_3952 ( pdhA2 ), SACE_3953 ( pdhB1 ), SACE_3954 ( bkdC2 ), SACE_4581 ( citE3 ), SACE_6118 ( pyc ), SACE_6636 (isocitrate dehydrogenase)...”
BH2299 aconitate hydratase from Bacillus halodurans C-125
50% identity, 98% coverage
DJ41_1103 aconitate hydratase AcnA from Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841
50% identity, 99% coverage
- Metabolic Responses to Polymyxin Treatment in Acinetobacter baumannii ATCC 19606: Integrating Transcriptomics and Metabolomics with Genome-Scale Metabolic Modeling
Zhu, mSystems 2019 - “...were upregulated after 2-mg/liter colistin treatment at 1h, including citrate synthase (DJ41_3568, 1.5-fold), aconitate hydratase (DJ41_1103, 2.7-fold), 2-oxoglutarate dehydrogenase (DJ41_3573 and DJ41_3574, 1.9 and 2.5-fold, respectively), succinyl-CoA ligase (DJ41_3576 and DJ41_3577, 2.1-fold for both), fumarate hydratase (DJ41_227, 2.6-fold), and malate dehydrogenase (DJ41_3006, 1.8-fold). However, the fluxes...”
SERP0921 aconitate hydratase from Staphylococcus epidermidis RP62A
Q5HPJ0 Aconitate hydratase A from Staphylococcus epidermidis (strain ATCC 35984 / DSM 28319 / BCRC 17069 / CCUG 31568 / BM 3577 / RP62A)
50% identity, 97% coverage
- Concentration-Dependent Global Quantitative Proteome Response of Staphylococcus epidermidis RP62A Biofilms to Subinhibitory Tigecycline
Sung, Cells 2022 - “...soluble modulin 1 1000.00 1000.00 0.00 SERP0852 recA fig|176279.9.peg.831 L Recombinase A 0.00 0.00 1000.00 SERP0921 acnA fig|176279.9.peg.898 C Aconitate hydratase A 1000.00 1.25 1.16 SERP0964 asd fig|176279.9.peg.942 E Aspartate-semialdehyde dehydrogenase 0.00 0.00 1000.00 cells-11-03488-t004_Table 4 Table 4 Differentially expressed proteins associated with quorum sensing. Fold...”
- The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus
Rochat, Nucleic acids research 2018 - “...In S. epidermidis, sucD, ylbP ( SERP0741 ), traP ( SERP1369 ), rocD, citB ( SERP0921 ), yjcG ( SERP0604 ) and icaR mRNAs are among the best matches of SD regions targeted by RsaE ( Supplementary Table S7 ). Although B. subtilis is distantly related...”
- Silver coordination polymers for prevention of implant infection: thiol interaction, impact on respiratory chain enzymes, and hydroxyl radical induction
Gordon, Antimicrobial agents and chemotherapy 2010 - “...SERP1709 SERP1710 TCA cycle SERP1258 SERP1387 SERP0921 SERP0985 SERP0813 Iron homeostasis SERP1120 SERP2127 SERP2126 SERP0292 SERP0291 SERP0290 SERP0293...”
- Phenotypic Modulation of Biofilm Formation in a Staphylococcus epidermidis Orthopedic Clinical Isolate Grown Under Different Mechanical Stimuli: Contribution From a Combined Proteomic Study
Bottagisio, Frontiers in microbiology 2020 - “...dehydrogenase (0.000989) Q5HNL0_STAEQ Q5HNL0 Citrate synthase (0.0016) Q5HQ26_STAEQ Q5HQ26 Succinate dehydrogenase, flavoprotein subunit (0.000326) ACNA_STAEQ Q5HPJ0 Aconitate hydratase A (0.00408) GLMS_STAEQ Q5HM69 Glutaminefructose-6-phosphate aminotransferase (0.00898) GLMS_STAEQ Q5HM69 Glutaminefructose-6-phosphate aminotransferase (0.0000149) GLMS_STAEQ Q5HM69 Glutaminefructose-6-phosphate aminotransferase (0.00177) NAGB_STAEQ Q5HRH8 Glucosamine-6-phosphate deaminase (0.00333) ALD1_STAEQ Q5HMA0 Aldehyde dehydrogenase SERP1729 (0.00172)...”
FRAAL2064 aconitate hydratase 1 from Frankia alni ACN14a
51% identity, 95% coverage
- The Proteogenome of Symbiotic Frankia alni in Alnus glutinosa Nodules
Pujic, Microorganisms 2022 - “...(FRAAL3889). Several TCA cycle proteins such as succinyl-CoA synthase (FRAAL1156, FC = 6.14), aconitate hydratase (FRAAL2064, FC = 5.44), malate dehydrogenase (FRAAL5612, FC = 4.37) or fumarase (FRAAL6152, FC = 3.96) were more abundant in nodules, but several others were not detected. There were 15 ribosomal...”
- “...5.67 - f 0.000 WP_011602534.1 FRAAL1337 pkn Serine/threonine protein kinase 5.50 T F 0.000 WP_011603231.1 FRAAL2064 acnA Aconitate hydratase AcnA 5.44 C A 0.006 WP_011605678.1 FRAAL4561 sufE (2Fe-2S)-Binding protein SufE 5.40 P A 0.001 WP_050997107.1 FRAAL2825 Putative N-fatty-acyl-amino acid synthase 5.38 E A 0.001 WP_011606156.1 FRAAL5045...”
lpp1659 Aconitate hydratase from Legionella pneumophila str. Paris
50% identity, 98% coverage
- The Legionella pneumophila genome evolved to accommodate multiple regulatory mechanisms controlled by the CsrA-system
Sahr, PLoS genetics 2017 - “...complex, Pyc (Lpp0531), Pyruvate carboxyltransferase, Ppc (Lpp1572) Phosphoenolpyruvate carboxylase, SfcA (Lpp3043), NAD-specific malic enzyme, AcnA (LPP1659), Aconitate hydratase, Icd (Lpp0878), Isocitrate dehydrogenase, Sdh (Lpp0595), Succinate dehydrogenase, Suc (Lpp0597), 2-Oxoglutarate dehydrogenase, Ald (Lpp0986), Alanine dehydrogenase, SdaA (Lpp0854), Serine dehydratase, PhbB (Lpp0621), acetoacetyl-CoA reductase, PhbC (Lpp2038), Polyhydroxyalkanoate synthase,...”
- Legionella pneumophila type II secretome reveals unique exoproteins and a chitinase that promotes bacterial persistence in the lung
DebRoy, Proceedings of the National Academy of Sciences of the United States of America 2006 - “...lpl2569 Y Y lpg0873 lpg1690 lpg2025 lpg1340 lpg2622 lpp0936 lpp1659 lpp2007 lpp1294 lpp1177 lpp2675 lpl0906 lpl1653 lpl2002 lpl1293 lpl1183 lpl2547 Y N N N N Y...”
RL4536 putative aconitate hydratase from Rhizobium leguminosarum bv. viciae 3841
50% identity, 98% coverage
PA14_44290 aconitate hydratase 1 from Pseudomonas aeruginosa UCBPP-PA14
51% identity, 98% coverage
IYO_RS10265 aconitate hydratase AcnA from Pseudomonas syringae pv. actinidiae ICMP 18884
52% identity, 98% coverage
MPAO1_17965, PAK_03790 aconitate hydratase AcnA from Pseudomonas aeruginosa PAK
Q9I3F5 Aconitate hydratase A from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PA1562 aconitate hydratase 1 from Pseudomonas aeruginosa PAO1
51% identity, 98% coverage
- An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in Pseudomonas aeruginosa MPAO1
Varadarajan, NPJ biofilms and microbiomes 2020 - “...role in biofilm formation. These included MuiA (MPAO1_18330) 46 , CbpD (MPAO1_21730) 47 , AcnA (MPAO1_17965) 48 and PilY1 (MPAO1_24155) 49 (Fig. 6 a, Table 3 ; see Discussion section). In addition, MPAO1_19625 was highly upregulated in biofilms (Fig. 6 a). Notably, its PAO1 homolog AprX...”
- “...MPAO1_27435 Amino acid ABC transporter substrate-binding protein 3.09 0.03 MPAO1_05385 DUF1302 domain-containing protein 2.80 0.03 MPAO1_17965 acnA * Aconitate hydratase 1.49 0.01 48 MPAO1_24155 pilY1 * Type 4a pilus biogenesis protein PilY1 1.54 0.01 49 MPAO1_05375 Fatty acid--CoA ligase 5.06 0.01 MPAO1_04650 OmpW family protein 1.49...”
- The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism
Rossello, Scientific reports 2017 - “...5.3 0.0143 PAK_03754 HtpG PA1596 4-hydroxyphenylpyruvate dioxygenase 4.5 0.0051 PAK_04521 Hpd PA0865 Aconitatehydratase 3.8 0.0114 PAK_03790 AcnA PA1562 30S ribosomal protein S13 2.8 0.0004 PAK_00930 RpsM PA4241 Polyhydroxyalkanoate synthesis protein PhaF 2.7 0.0172 PAK_05567 PhaF PA5060 Large-conductance mechano sensitive channel 2.2 0.0029 PAK_05082 MscL PA4614 50S...”
- Recombinant Actifensin and Defensin-d2 Induce Critical Changes in the Proteomes of Multidrug-Resistant Pseudomonas aeruginosa and Candida albicans
Gbala, Microbiology spectrum 2022 - “...protein S3 Up Non 13 sp/ Q9I244 /EFG2_PSEAE Elongation factor G2 Down Non 14 sp/ Q9I3F5 /ACNA_PSEAE Aconitate hydratase A Down Non 15 sp/ Q9I467 /COBQ_PSEAE Cobyric acid synthase Down Non 16 tr/ AAG07261 /G3XD87_PSEAE Respiratory nitrate reductase chain Down Non 17 tr/ Q9HUY5 /Q9HUY5_PSEAE Magnesium-transporting...”
- Protein Interaction Networks of Catalytically Active and Catalytically Inactive PqsE in Pseudomonas aeruginosa
Taylor, mBio 2022 - “...ybeY ybeY 2.956800118 Q02LM8 kynB kynB 1.616357697 Q02DL4 thrB thrB 1.365692537 Q02FF4 ureA ureA 1.264836648 Q9I3F5 acnA acnA 1.002888279 Q02V73 glyS glyS 1.008682243 Q59638 aceF aceF 1.067938829 B7UZY2 cysD cysD 1.092340172 Q02H29 murC murC 1.224317298 P27726 gap gapA 1.354759487 Q51363 nadB nadB 1.354759487 Q9I6M4 davT PA14_03450...”
- Over-Expression of Hypochlorite Inducible Major Facilitator Superfamily (MFS) Pumps Reduces Antimicrobial Drug Susceptibility by Increasing the Production of MexXY Mediated by ArmZ in Pseudomonas aeruginosa
Dulyayangkul, Frontiers in microbiology 2020 - “...<0.05 0.002 <0.05 0.002 Q9I314 Type III export protein PscJ PscJ 11.57 0.002 6.07 0.010 Q9I3F5 Aconitate hydratase A AcnA <0.05 0.000 <0.05 0.000 Q9I3L9 Sulfate-binding protein of ABC transporter CysP <0.05 0.002 <0.05 0.002 Q9I3Q1 Uncharacterized protein PA1450 >20 0.001 >20 0.001 Q9I427 Cytochrome bo(3)...”
- Proteomic Analysis of Resistance of Gram-Negative Bacteria to Chlorhexidine and Impacts on Susceptibility to Colistin, Antimicrobial Peptides, and Ceragenins
Hashemi, Frontiers in microbiology 2019 - “...the formation of citrate (and CoA) from oxaloacetate and acetyl-CoA. Tricarboxylic acid cycle 316.01 32 Q9I3F5 Aconitate hydratase A (AcnA) Catabolism of short chain fatty acids via TCA cycle, 2-methylcitrate cycle I, catalyzes isomerization of citrate to isocitrate. Anaerobic respiration, propionate metabolic process (methylcitrate cycle), response...”
- Metaproteomics reveals major microbial players and their biodegradation functions in a large-scale aerobic composting plant
Liu, Microbial biotechnology 2015 - “...Streptococcus suis Bacilli; Lactobacillales Aconitate hydratase 2 Q9I2V5 TCA Pseudomonas aeruginosa Gammaproteobacteria Aconitate hydratase 1 Q9I3F5 TCA Pseudomonas aeruginosa Gammaproteobacteria Isocitrate lyase Q9I0K4 TCA Pseudomonas aeruginosa Gammaproteobacteria Malate synthase G Q3K5N4 TCA Pseudomonas fluorescens Gammaproteobacteria Malate dehydrogenase Q4FQU7 TCA Psychrobacter arcticus Gammaproteobacteria Isocitrate dehydrogenase [NADP] 2...”
- PtsN in Pseudomonas aeruginosa Is Phosphorylated by Redundant Upstream Proteins and Impacts Virulence-Related Genes
Underhill, Journal of bacteriology 2023 (secret) - Cold atmospheric pressure plasma-antibiotic synergy in Pseudomonas aeruginosa biofilms is mediated via oxidative stress response
Maybin, Biofilm 2023 - “...downregulation of fumarate hydratase class II 2 (PA4470) (log 2 FC 1.03), aconitate hydratase A (PA1562) (log 2 FC 1.04), citrate synthase (PA0795) (log 2 FC 1.06), and isocitrate lyase (PA2634) (log 2 FC 1.09) (data not shown). Additionally, differential expression at 6h post-treatment suggest recovery...”
- Sodium Selenite Enhances Antibiotics Sensitivity of Pseudomonas aeruginosa and Deceases Its Pathogenicity by Inducing Oxidative Stress and Inhibiting Quorum Sensing System
Kong, Antioxidants (Basel, Switzerland) 2021 - “...zwf 1.1942 1.68 10 2 Glucose-6-phosphate 1-dehydrogenase PA4366 sodB 2.2508 7.69 10 23 superoxide dismutase PA1562 acnA 2.5174 1.32 10 40 aconitate hydratase 1 PA5344 oxyR 0.9082 2.24 10 1 OxyR PA4613 katB 2.6860 2.03 10 39 catalase PA2147 katE 1.9478 2.38 10 4 catalase HPII...”
- The LysR-Type Transcriptional Regulator BsrA (PA2121) Controls Vital Metabolic Pathways in Pseudomonas aeruginosa
Modrzejewska, mSystems 2021 - “...transport ( PA3233 and PA3234 ) ( 70 ) and acetate pathways ( acsA [ PA1562 ], acsB [ PA1787 ], and exaC [ PA1984 ]), encoding probable succinyl-CoA/acetate CoA-transferase ( PA5445 ) ( 71 ), also showed reduced expression (FC between 2 and 1.5) in...”
- Transcriptomic Analysis Reveals the Dependency of Pseudomonas aeruginosa Genes for Double-Stranded RNA Bacteriophage phiYY Infection Cycle
Zhong, iScience 2020 - “...3.153 3.292 PA1176 9.204 5.897 PA2849 2.968 3.453 PA3337 8.085 11.150 7.694 3.026 7.299 8.960 PA1562 3.315 3.669 3.225 3.095 PA1864 10.768 11.763 25.108 3.205 6.056 11.834 PA3879 5.178 6.564 7.127 4.221 6.887 6.211 PA4238 3.010 2.322 PA3609 10.840 11.843 8.087 8.310 PA5239 4.160 3.332 2.745...”
- NtrBC Regulates Invasiveness and Virulence of Pseudomonas aeruginosa During High-Density Infection
Alford, Frontiers in microbiology 2020 - “...protein 1.54 1.56 PA0854 fumC2 Fumarate hydratase 1.52 1.85 PA1326 ilvA2 Threonine dehydratase 3.84 3.45 PA1562 acnA Aconitate hydratase 1.39 2.07 PA1581 sdhC Succinate dehydrogenase, cytochrome b556 subunit 2.71 1.91 PA1582 sdhD Succinate dehydrogenase (D subunit) 2.20 2.23 PA1583 sdhA Succinate dehydrogenase flavoprotein subunit 1.70 2.00...”
- Reverse diauxie phenotype in Pseudomonas aeruginosa biofilm revealed by exometabolomics and label-free proteomics
Yung, NPJ biofilms and microbiomes 2019 - “...NR NR AccB PA4847 Acetyl-CoA carboxylase biotin carboxyl carrier protein NR NR NR NR AcnA PA1562 Aconitate hydratase NR NR NR NR AcnB PA1787 Aconitate hydratase B NR NR o NR NR AotP PA0892 Arginine/ornithine ABC transporter ATP-binding protein NR NR NR NR CcpA PA4587 Cytochrome...”
- Proteomic Response of Pseudomonas aeruginosa PAO1 Adhering to Solid Surfaces
Guilbaud, Frontiers in microbiology 2017 - “...periplasmic nitrate reductase protein NapA PA1174 napA 2.8 1.6 8.0 7.3E-04 7.7E-06 aconitate hydratase 1 PA1562 acnA 12.6 15.6 21.2 1.9E-03 urocanate hydratase PA5100 hutU 0.0 0.0 3.2 8.6E-06 1.1E-05 probable dihydrolipoamide acetyltransferase PA3415 0.8 0.0 4.6 3.0E-04 1.5E-07 quinone oxidoreductase PA0023 qor 1.6 0.6 5.2...”
- More
IX87_RS16955 aconitate hydratase AcnA from Acinetobacter baumannii
50% identity, 99% coverage
SXYL_01534 aconitate hydratase AcnA from Staphylococcus xylosus
51% identity, 98% coverage
Q39WW6 Aconitate hydratase from Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
52% identity, 97% coverage
Psyr_3404 Aconitate hydratase 1 from Pseudomonas syringae pv. syringae B728a
52% identity, 98% coverage
- Gene Expression Profiling in Viable but Nonculturable (VBNC) Cells of Pseudomonas syringae pv. syringae
Postnikova, Frontiers in microbiology 2015 - “...are activated in the VBNC mode (Table 1 ). Gene encoding aconitate hydratase or aconitase (Psyr_3404) from the same category ( Energy generation ) is also up-regulated (Table 1 ). Aconitase is an iron-sulfur protein, which catalyses the reversible isomerization of citrate and isocitrate via cis...”
- “...Psyr_0905 1.73 Histidine kinase, HAMP Chemosensing & chemotaxis Psyr_2215 1.65 CheW-like protein Chemosensing & chemotaxis Psyr_3404 1.87 acnA, aconitate hydratase Energy generation Psyr_3204 1.02 NADH dehydrogenase subunit I Energy generation Psyr_3201 1.03 NADH dehydrogenase I subunit F Energy generation Psyr_3198 1.08 NADH dehydrogenase subunit B Energy...”
ABUW_3346 aconitate hydratase AcnA from Acinetobacter baumannii
50% identity, 99% coverage
PLES_37671 aconitate hydratase 1 from Pseudomonas aeruginosa LESB58
51% identity, 98% coverage
Atu2685 aconitate hydratase from Agrobacterium tumefaciens str. C58 (Cereon)
50% identity, 98% coverage
WP_042362767 aconitate hydratase AcnA from Staphylococcus xylosus
51% identity, 98% coverage
B9DP54 Aconitate hydratase from Staphylococcus carnosus (strain TM300)
51% identity, 98% coverage
CLIBASIA_01680 aconitate hydratase from Candidatus Liberibacter asiaticus str. psy62
47% identity, 99% coverage
NCLIV_046260 Iron regulatory protein-like protein, related from Neospora caninum Liverpool
48% identity, 99% coverage
- The 2-methylcitrate cycle is implicated in the detoxification of propionate in Toxoplasma gondii
Limenitakis, Molecular microbiology 2013 - “...Tp PrpE NCLIV_032540 ETH_00007195 PrpC NCLIV_024780 ETH_00026655 PrpD NCLIV_013410 ETH_00015105 PrpB NCLIV_000780 Contig 1606+713 Acn NCLIV_046260 ETH_00020300 PF13_0229 TP01_1050 NCLIV_033680 ETH_00021115 PF08_0031 cgd1_600 TP02_0450 Abbreviations Bb Pm Pt Tt PrpE XP_001460124 XP_001025576 PrpC XP_002787782 XP_001447471 XP_001023516 PrpD EER04808 XP_001457033 EAS06482 PrpB XP_002772756 XP_001459690 EAR89396 Acn BBOV_III011790...”
YPTB2143 aconitate hydratase 1 from Yersinia pseudotuberculosis IP 32953
50% identity, 97% coverage
- IscR is essential for yersinia pseudotuberculosis type III secretion and virulence
Miller, PLoS pathogens 2014 - “...grxA 2.3 YPTB1418 integration host factor beta-subunit ihfB 2.7 YPTB2047 pyruvate kinase II pykA 2.3 YPTB2143 aconitate hydratase 1 acnA 2.2 YPTB2216 putative acetolactate synthase large subunit ilvB 2.2 YPTB2217 putative acetolactate synthase small subunit ilvN 2.3 YPTB2306 pyruvate kinase I pykF 2.0 YPTB2845 nucleoside diphosphate...”
XAC1882 aconitase from Xanthomonas axonopodis pv. citri str. 306
51% identity, 97% coverage
XP_001451848 uncharacterized protein from Paramecium tetraurelia
49% identity, 97% coverage
Swit_2731 aconitase from Sphingomonas wittichii RW1
50% identity, 98% coverage
- Identification of genes potentially involved in solute stress response in Sphingomonas wittichii RW1 by transposon mutant recovery
Coronado, Frontiers in microbiology 2014 - “...other transposon insertions were located in genes Swit_2730, annotated as a hypothetical protein (clone 84), Swit_2731 coding for an aconitase-domain protein (clone 298), Swit_2958, coding for a BadM/Rrf2 family transcriptional regulator (clone 6-D11) and one with an insertion in the intergenic region between Swit_3114 and Swit_3115,...”
- “...synthase C, RluA family 0.0280.002 0.0110.001 2.6 2.4 2.5 84 Swit_2730 pRL27 Hypothetical protein 298 Swit_2731 pRL27 Aconitase domain protein 6-E3 Intergenic Swit_3114-Swit_3115 pRL27 Hypothetical protein Ribosomal protein L36, rpmJ 0.0420.004 0.0300.001 1.6 1 1.3 6-D11 Swit_2958 pRL27 BadM/Rrf2 family transcriptional regulator 0.0570.0009 0.0260.0005 1 1...”
- Proteomic profiling of the dioxin-degrading bacterium Sphingomonas wittichii RW1
Colquhoun, Journal of biomedicine & biotechnology 2012 - “...surface antigen-like protein 1.09 0.82 648 148550878 Swit_4921 3-Keto-5-aminohexanoate cleavage enzyme 1.15 0.45 177 148555643 Swit_2731 Aconitate hydratase 1 1.23 0.44 479 148550877 Swit_4920 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 1.57 0.5 937 148550856 Swit_4897 Dioxin dioxygenase, alpha subunit 1.59 0.38 a Arbitrary identifier for spot location (see...”
DDB_G0279159 aconitate hydratase from Dictyostelium discoideum AX4
50% identity, 97% coverage
BC33_RS15515 aconitate hydratase AcnA from Escherichia coli ATCC 700728
ECs1849 aconitate hydrase 1 from Escherichia coli O157:H7 str. Sakai
51% identity, 97% coverage
- Transcriptomic analysis reveal differential gene expressions of Escherichia coli O157:H7 under ultrasonic stress
Li, Ultrasonics sonochemistry 2021 - “...Major metabolic pathways involved in differentially expressed genes. Gene ID Genes Ratio Description TCA cycle BC33_RS15515 acnA 3.1 aconitate hydratase BC33_RS22310 acnB 1.5 aconitate hydratase 2 BC33_RS19240 sucA 2.4 2-oxoglutarate dehydrogenase E1 component BC33_RS19235 sucB 2.2 2-oxoglutarate dehydrogenase E2 component BC33_RS19230 sucC 2.5 succinyl-CoA synthetase beta...”
- Physiological Response of Escherichia coli O157:H7 Sakai to Dynamic Changes in Temperature and Water Activity as Experienced during Carcass Chilling
King, Molecular & cellular proteomics : MCP 2016 - “...energy metabolism, including genes of the TCA cycle ( gltA / ECs0745 , acnA / ECs1849 , fumC / ECs2317 ). The T-profiler analysis of the transcriptomic data also revealed upregulation of the TCA cycle from 1605 min onwards ( supplemental Table S14 ). Genes involved...”
- Global transcriptional response of Escherichia coli O157:H7 to growth transitions in glucose minimal medium
Bergholz, BMC microbiology 2007 - “...yciG 2.91 2 ECs2430 ydiZ 2.09 2 ECs1848 - 2.73 2 ECs2450 ydjS 3.94 2 ECs1849 acnA 4.07 2 ECs2451 astB 4.11 2 ECs1858 yciR 3.14 2 ECs2452 astD 5.88 2 ECs1874 ycjK 4.73 2 ECs2453 astA 4.62 2 ECs1875 ycjL 5.14 2 ECs2454 astC 6.97...”
- Predicting candidate genomic sequences that correspond to synthetic functional RNA motifs
Laserson, Nucleic acids research 2005 - “...Most interestingly, the 39 nt sequences 1720 are identical copies found in hypothetical genes (yciQ, ECs1849, Z2542) of four different E.coli species. It is unlikely that candidate sequences 7/8 and 17/18/19/20 occur by chance. Table 3 shows that several other mostly shorter sequences (23, 1315, 2124,...”
EIO_1377, KVH_06620, KVU_0869, KvSKV_06590 aconitate hydratase AcnA from Ketogulonicigenium vulgare WSH-001
50% identity, 98% coverage
- Reconstruction and analysis of carbon metabolic pathway of Ketogulonicigenium vulgare SPU B805 by genome and transcriptome
Wang, Scientific reports 2018 - “...TCA 2.3.3.1 KVC_2235 KvSKV_10775 KVH_10860 KVU_1659 EIO_2099 Acn TCA 4.2.1.3 KVC_0695, 1409 KvSKV_06590 KVH_06620 KVU_0869 EIO_1377 Icd TCA 1.1.1.42 KVC_1885 KvSKV_08965 KVH_09010 KVU_1326 EIO_1866 SucA TCA 1.2.4.2 KVC_0103 KvSKV_01120 KVH_01115 KVU_2455 EIO_0115 SucDC TCA 6.2.1.5 KVC_0104 KvSKV_11895 KVH_01125 KVU_2456, 2457 EIO_0116, 0117 SdhA TCA 1.3.5.4 KVC_0109-0111,...”
- “...1169 GltA TCA 2.3.3.1 KVC_2235 KvSKV_10775 KVH_10860 KVU_1659 EIO_2099 Acn TCA 4.2.1.3 KVC_0695, 1409 KvSKV_06590 KVH_06620 KVU_0869 EIO_1377 Icd TCA 1.1.1.42 KVC_1885 KvSKV_08965 KVH_09010 KVU_1326 EIO_1866 SucA TCA 1.2.4.2 KVC_0103 KvSKV_01120 KVH_01115 KVU_2455 EIO_0115 SucDC TCA 6.2.1.5 KVC_0104 KvSKV_11895 KVH_01125 KVU_2456, 2457 EIO_0116, 0117 SdhA TCA...”
- “...GltA TCA 2.3.3.1 KVC_2235 KvSKV_10775 KVH_10860 KVU_1659 EIO_2099 Acn TCA 4.2.1.3 KVC_0695, 1409 KvSKV_06590 KVH_06620 KVU_0869 EIO_1377 Icd TCA 1.1.1.42 KVC_1885 KvSKV_08965 KVH_09010 KVU_1326 EIO_1866 SucA TCA 1.2.4.2 KVC_0103 KvSKV_01120 KVH_01115 KVU_2455 EIO_0115 SucDC TCA 6.2.1.5 KVC_0104 KvSKV_11895 KVH_01125 KVU_2456, 2457 EIO_0116, 0117 SdhA TCA 1.3.5.4...”
- “...EIO_1168, 1169 GltA TCA 2.3.3.1 KVC_2235 KvSKV_10775 KVH_10860 KVU_1659 EIO_2099 Acn TCA 4.2.1.3 KVC_0695, 1409 KvSKV_06590 KVH_06620 KVU_0869 EIO_1377 Icd TCA 1.1.1.42 KVC_1885 KvSKV_08965 KVH_09010 KVU_1326 EIO_1866 SucA TCA 1.2.4.2 KVC_0103 KvSKV_01120 KVH_01115 KVU_2455 EIO_0115 SucDC TCA 6.2.1.5 KVC_0104 KvSKV_11895 KVH_01125 KVU_2456, 2457 EIO_0116, 0117 SdhA...”
UTI89_C1547 aconitate hydratase 1 from Escherichia coli UTI89
50% identity, 99% coverage
- Glycosuria Alters Uropathogenic Escherichia coli Global Gene Expression and Virulence
Islam, mSphere 2022 - “...0.0688 1.5421* 1.5935* Succinate-CoA ligase subunit alpha UTI89_C1438 adhE 0.0074 2.0939* 2.1154* Bifunctional acetaldehyde-CoA/alcohol dehydrogenase UTI89_C1547 acnA 0.0207 1.8463* 1.8493* Aconitate hydratase AcnA UTI89_C1619 nifJ b 0.6094 0.5765 1.1706* Pyruvate:ferredoxin (flavodoxin) oxidoreductase UTI89_C1799 fumC 2.1357* 0.6150 2.7316* Class II fumarate hydratase UTI89_C1800 fumB 0.1454 3.0780* 3.2048*...”
Acn / b1276 aconitate hydratase 1 from Escherichia coli K-12 substr. MG1655 (see 16 papers)
acnA / P25516 aconitate hydratase 1 from Escherichia coli (strain K12) (see 15 papers)
ACNA_ECOLI / P25516 Aconitate hydratase A; ACN; Aconitase; Iron-responsive protein-like; IRP-like; RNA-binding protein; Stationary phase enzyme; EC 4.2.1.3 from Escherichia coli (strain K12) (see 12 papers)
P25516 aconitate hydratase (EC 4.2.1.3) from Escherichia coli (see paper)
acnA aconitate hydratase 1; EC 4.2.1.3 from Escherichia coli K12 (see 6 papers)
b1276 aconitate hydratase from Escherichia coli str. K-12 substr. MG1655
NP_415792 aconitate hydratase 1 from Escherichia coli str. K-12 substr. MG1655
50% identity, 99% coverage
- function: Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. The apo form of AcnA functions as a RNA- binding regulatory protein which plays a role as a maintenance or survival enzyme during nutritional or oxidative stress. During oxidative stress inactive AcnA apo-enzyme without iron sulfur clusters binds the acnA mRNA 3' UTRs (untranslated regions), stabilizes acnA mRNA and increases AcnA synthesis, thus mediating a post- transcriptional positive autoregulatory switch. AcnA also enhances the stability of the sodA transcript.
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer.
disruption phenotype: Cells lacking this gene are more sensitive to peroxide stress. The acnAB double mutant does not grow on unsupplemented glucose minimal medium and does not respond under aerobic conditions to glutamate. The acnAB double mutant retains a low but significant aconitase activity. - Adaptive laboratory evolution and shuffling of Escherichia coli to enhance its tolerance and production of astaxanthin
Lu, Biotechnology for biofuels and bioproducts 2022 - “...protease Lys554fs a 1.220.01 cusB (b0574) Copper/silver export system membrane fusion protein Pro103Ser 1.110.05 acnA (b1276) Aconitate hydratase 1 Ser522Gly 1.090.01 rcsC (b2218) Sensory histidine kinase RcsC Phe136Leu 1.450.07 Membrane and transport protein pqiA (b0950) Intermembrane transport protein PqiA Met163Ile 1.170.08 fixA (b0041) Putative electron transfer...”
- Metabolic shift of Escherichia coli under salt stress in the presence of glycine betaine
Metris, Applied and environmental microbiology 2014 - “...b3417 b3417 b3783 b2041 b3306 b2890 b1274 b4015 b2296 b1276 b1000 b2266 b0757 b0759 b3431 b2053 b0220 b4014 b4014 b2684 b0678 b1482 b4376 b1896 b1897 b0133...”
- 18th Congress of the European Hematology Association, Stockholm, Sweden, June 13–16, 2013
, Haematologica 2013 - Evolutionary dynamics of small RNAs in 27 Escherichia coli and Shigella genomes
Skippington, Genome biology and evolution 2012 - “...bfr b3336 ryhB 4 Variable Congruent No fumA b1612 ryhB 27 Core Incongruent Yes acnA b1276 ryhB 23 Variable Incongruent No ftnA b1905 ryhB 27 Core Incongruent No sodB b1656 ryhB 27 Core Incongruent No sdhA b0723 ryhB 27 Core Incongruent No sdhC b0721 ryhB 27...”
- Accurate estimation of nucleic acids by amplification efficiency dependent PCR
Chatterjee, PloS one 2012 - “...(Oh et al data [40] ) (C) b0720 citrate synthase 1.92 1.93 1.95 1.96 1.79 b1276 aconitate hydratase, acnA 1.90 1.80 4.66 1.06 1.25 b0118 aconitate hydratase, acnB 1.95 1.96 1.26 1.32 2.17 b1136 isocitrate dehydrogenase 1.80 1.87 0.60 0.93 1.32 b4016 isocitrate dehydrogenase kinase 1.91...”
- Genome-scale gene/reaction essentiality and synthetic lethality analysis
Suthers, Molecular systems biology 2009 - “...(1985) D-Ala gutQ (b2708), yrbH (b3197) Disjoint pair Meredith and Woodard (2005) D-arabinose 5-phosphate acnA (b1276), acnB (b0118) Disjoint pair Gruer et al (1997) Glutamate tktA (b2935), tktB (b2465) Disjoint pair Zhao and Winkler (1994) Aromatic amino acids and Vitamin B 6 cynT (b0339), YadF (b0126)...”
- Genome-wide transcriptional responses of Escherichia coli K-12 to continuous osmotic and heat stresses
Gunasekera, Journal of bacteriology 2008 - “...11, 2017 by University of California, Berkeley A; b1276), bfr (bacterioferritin; b3336), ibpA (heat shock chaperone; b3687), and degP (heat shock serine...”
- An evaluation of Comparative Genome Sequencing (CGS) by comparing two previously-sequenced bacterial genomes
Herring, BMC genomics 2007 - “...folD b0529 a>t yes SNP new 1092487 ycdT b1025 c>t yes SNP known 1335418 acnA b1276 a>g yes SNP known 1650355 intQ b1579 t>c yes SNP known 2030447 yedJ b1962 g>a yes SNP known 2865477 rpoS b2741 g>a yes ROI known 3484227 crp b3357 c>a yes...”
- More
- Cooperativity of two active sites in bacterial homodimeric aconitases.
Tsuchiya, Biochemical and biophysical research communications 2009 (PubMed)- GeneRIF: The structural state of AcnA by the chemical crosslinking and small-angle X-ray scattering was analyzed.
- The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn).
Prodromou, Journal of general microbiology 1991 (PubMed)- GeneRIF: N-terminus verified by Edman degradation on mature peptide
- Quantitative proteomic analysis reveals the influence of plantaricin BM-1 on metabolic pathways and peptidoglycan synthesis in Escherichia coli K12
Wang, PloS one 2020 - “...TCA cycle P61889 -1.24 0.000474 Mdh Malate dehydrogenase, NAD(P)-binding Enzyme; Energy metabolism, carbon: TCA cycle P25516 -2.10 0.000075 AcnA Aconitate hydratase 1 Enzyme; Energy metabolism, carbon: TCA cycle P33940 1.80 0.001449 Mqo Unknown Malate dehydrogenase, FAD/NAD(P)-binding domain P14407 1.60 0.000371 FumB Anaerobic class I fumarate hydratase...”
- A Sinorhizobium meliloti RpoH-Regulated Gene Is Involved in Iron-Sulfur Protein Metabolism and Effective Plant Symbiosis under Intrinsic Iron Limitation
Sasaki, Journal of bacteriology 2016 - “...E. coli and S. meliloti, respectively), acnA (aconitase; 62%; P25516 and CAC47808), gltB ( subunit of GltS; 45%; P09831 and CAC47390), and gltD ( subunit of...”
- The T4 phage DNA mimic protein Arn inhibits the DNA binding activity of the bacterial histone-like protein H-NS
Ho, The Journal of biological chemistry 2014 - “...Protein translocase subunit SecA Aldehyde-alcohol dehydrogenase P25516 B1XCM5 P06612 P33602 C4ZRJ3 P0A9Q7 10,868/179 4,534/66 4,327/80 4,242/70 3,471/61...”
- Genome-scale identification and characterization of moonlighting proteins
Khan, Biology direct 2014 - “...moonlighting proteins (Figure 7 A), which consists of four moonlighting proteins (P77489, P0A8Q3, P0AC47, and P25516). Then, similar to the analysis in Figure 5 B and 5 C, we computed functional clustering profile for co-expressed genes of E. coli moonlighting proteins to see if co-expressed genes...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...5.79/41,417 (DIGE 4.5-6.5) 5.72/31,846 (5-6) AcnA P25516 Aconitate hydratase 1 5.59/97,516.00 AcnB P36683 Aconitate hydratase 2 5.24/93,498.11 5.30/95,000...”
XP_002765934 aconitase, putative from Perkinsus marinus ATCC 50983
50% identity, 96% coverage
y2063 aconitate hydrase 1 from Yersinia pestis KIM
50% identity, 97% coverage
- Deletion of Yersinia pestis ail Causes Temperature-Sensitive Pleiotropic Effects, Including Cell Lysis, That Are Suppressed by Carbon Source, Cations, or Loss of Phospholipase A Activity
Kolodziejek, Journal of bacteriology 2021 - “...metabolism, carbon: glycolysis 1 2.5 y2165 gapA Glyceraldehydes-3-phosphate dehydrogenase Energy metabolism, carbon: glycolysis 1 4.2 y2063 acnA Aconitate hydratase Energy metabolism, carbon: TCA cycle 1 1.5 y1953 Hypothetical protein Unknown; belongs to glutaredoxin (GRX) family 1 10.7 y1802 icdA Isocitrate dehydrogenase Energy metabolism, carbon: TCA cycle...”
- Proteomic analysis of iron acquisition, metabolic and regulatory responses of Yersinia pestis to iron starvation
Pieper, BMC microbiology 2010 - “...33 y1990 tpx thiol peroxidase U 479 18655 5.13 3.02 3.06 0.986 0.816 1.198 34 y2063 acnA aconitate hydratase A CY RyhB 565 97825 6.08 - 0.22 < 0.05 N.D. < 0.05 35 y2255 yebC hypothetical protein y2255 U 219 39957 4.74 0.11 0.40 0.285 0.000...”
A0QX20 Aconitate hydratase A from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
MSMEG_3143 aconitate hydratase 1 from Mycobacterium smegmatis str. MC2 155
MSMEG_3143, MSMEI_3062 aconitate hydratase from Mycolicibacterium smegmatis MC2 155
49% identity, 97% coverage
- Interactome Analysis Identifies MSMEI_3879 as a Substrate of Mycolicibacterium smegmatis ClpC1
Ogbonna, Microbiology spectrum 2023 - “...domain-containing protein 0.030 3.000 A0QS62 rplJ , MSMEG_1364, MSMEI_1325 50S ribosomal protein L10 0.078 8.500 A0QX20 acnA , can , MSMEG_3143, MSMEI_3062 Aconitate hydratase A (ACN) (aconitase) (EC 4.2.1.3) 0.055 14.500 A0QR90 MSMEG_1029, MSMEG_2309 Probable transcriptional regulatory protein 0.095 7.500 A0QT07 sdhB , MSMEG_1669 Succinate dehydrogenase,...”
- “...0.06 0.05 A0QT07 sdhB , MSMEG_1669, MSMEI_1629 Succinate dehydrogenase, iron-sulfur protein (EC 1.3.99.1) 0.06 0.08 A0QX20 acnA , can , MSMEG_3143, MSMEI_3062 Aconitate hydratase A (ACN) (aconitase) (EC 4.2.1.3) 0.06 0.08 A0QVL2 mqo , MSMEG_2613, MSMEI_2551 Probable malate:quinone oxidoreductase 0.36 0.16 A0R2B1 kgd , sucA ,...”
- Interactome Analysis Identifies MSMEI_3879 as a Substrate of Mycolicibacterium smegmatis ClpC1
Ogbonna, Microbiology spectrum 2023 - “...rplJ , MSMEG_1364, MSMEI_1325 50S ribosomal protein L10 0.078 8.500 A0QX20 acnA , can , MSMEG_3143, MSMEI_3062 Aconitate hydratase A (ACN) (aconitase) (EC 4.2.1.3) 0.055 14.500 A0QR90 MSMEG_1029, MSMEG_2309 Probable transcriptional regulatory protein 0.095 7.500 A0QT07 sdhB , MSMEG_1669 Succinate dehydrogenase, iron-sulfur protein (EC 1.3.99.1) 0.059...”
- “...MSMEG_1669, MSMEI_1629 Succinate dehydrogenase, iron-sulfur protein (EC 1.3.99.1) 0.06 0.08 A0QX20 acnA , can , MSMEG_3143, MSMEI_3062 Aconitate hydratase A (ACN) (aconitase) (EC 4.2.1.3) 0.06 0.08 A0QVL2 mqo , MSMEG_2613, MSMEI_2551 Probable malate:quinone oxidoreductase 0.36 0.16 A0R2B1 kgd , sucA , MSMEG_5049, MSMEI_4922 Multifunctional 2-oxoglutarate metabolism...”
- The Benefits of Toxicity: M. smegmatis VapBC TA Module Is Induced by Tetracycline Exposure and Promotes Survival
Zamakhaev, Microorganisms 2023 - “...(pyruvate kinase MSMEG_3227, pyruvate dehydrogenase subunits MSMEG_2280, MSMEG_4323, MSMEG_0903), tricarboxylic acid cycle (e.g., aconitate hydratase MSMEG_3143, fumarate hydratase MSMEG_5240), and pentose-phosphate shunt (transketolase MSMEG_3103) of carbohydrate metabolism. At the same time, the abundance of citrate synthase MSMEG_5672, which is involved in glyoxylate shunt, was increased, whereas...”
- The Effect of Antimicrobial Photodynamic Inactivation on the Protein Profile of Dormant Mycolicibacterium smegmatis Containing Endogenous Porphyrins
Shashin, International journal of molecular sciences 2023 - “...sensitive for 5 min of illumination. Among the Krebs cycle, only two enzymes, aconitase ( MSMEG_3143 ) and citrate synthase I ( MSMEG_5672 ), were light-resistant. The majority of enzymes of the tricarboxylic acid cycle were found to be sensitive even to a small (5 min)...”
- Dynamic Characterization of Protein and Posttranslational Modification Levels in Mycobacterial Cholesterol Catabolism
Xu, mSystems 2020 - “...involved in the TCA cycle, glyoxylate shunt, methylcitrate cycle, and methylmalonyl pathway, including aconitate hydratase (MSMEG_3143), isocitrate lyase (MSMEG_0911), methylmalonyl-CoA epimerase (MSMEG_4921), methylmalonyl-CoA mutase (MSMEG_3158), methylisocitrate lyase (MSMEG_6646), and 2-methylcitrate dehydratase (MSMEG_6645), showed higher protein expression levels in the presence of cholesterol ( Fig.2B ). This...”
- Quantitative mass spectrometry reveals plasticity of metabolic networks in Mycobacterium smegmatis
Chopra, Molecular & cellular proteomics : MCP 2014 - “...conditions were identified as aconitate hydratase I (AcnA, MSMEG_3143) and isocitrate lyase 1 (Icl1, MSMEG_0911). Analysis of two more spots from the propionate...”
- “...of the TCA cycle enzymes. Aconitate hydratase (AcnA, MSMEG_3143) and the associated regulatory protein AcnR (MSMEG_3142) were both present at higher levels in...”
- Surface proteome of "Mycobacterium avium subsp. hominissuis" during the early stages of macrophage infection
McNamara, Infection and immunity 2012 - “...NA NA MSMEG_2351 NA NA NA NA NA NA MSMEG_3143 NA NA NA NA MSMEG_4290 MSMEG_5524 MSMEG_3811 MSMEG_2374 MSMEG_1583 MSMEG_1469 MSMEG_1365 NA MSMEG_3050 NA NA NA...”
- Interactome Analysis Identifies MSMEI_3879 as a Substrate of Mycolicibacterium smegmatis ClpC1
Ogbonna, Microbiology spectrum 2023 - “..., MSMEG_1364, MSMEI_1325 50S ribosomal protein L10 0.078 8.500 A0QX20 acnA , can , MSMEG_3143, MSMEI_3062 Aconitate hydratase A (ACN) (aconitase) (EC 4.2.1.3) 0.055 14.500 A0QR90 MSMEG_1029, MSMEG_2309 Probable transcriptional regulatory protein 0.095 7.500 A0QT07 sdhB , MSMEG_1669 Succinate dehydrogenase, iron-sulfur protein (EC 1.3.99.1) 0.059 6.500...”
- “...MSMEI_1629 Succinate dehydrogenase, iron-sulfur protein (EC 1.3.99.1) 0.06 0.08 A0QX20 acnA , can , MSMEG_3143, MSMEI_3062 Aconitate hydratase A (ACN) (aconitase) (EC 4.2.1.3) 0.06 0.08 A0QVL2 mqo , MSMEG_2613, MSMEI_2551 Probable malate:quinone oxidoreductase 0.36 0.16 A0R2B1 kgd , sucA , MSMEG_5049, MSMEI_4922 Multifunctional 2-oxoglutarate metabolism enzyme...”
P63434 Aconitate hydratase A from Staphylococcus aureus (strain MW2)
SA1184 aconitate hydratase from Staphylococcus aureus subsp. aureus N315
SAV1350 aconitate hydratase from Staphylococcus aureus subsp. aureus Mu50
SAOUHSC_01347 aconitate hydratase 1 from Staphylococcus aureus subsp. aureus NCTC 8325
SAUSA300_1246 aconitate hydratase from Staphylococcus aureus subsp. aureus USA300_FPR3757
NWMN_1263 aconitate hydratase from Staphylococcus aureus subsp. aureus str. Newman
SACOL1385 aconitate hydratase from Staphylococcus aureus subsp. aureus COL
SAFDA_1246 aconitate hydratase AcnA from Staphylococcus aureus
49% identity, 98% coverage
- Discovery of Furanoquinone Derivatives as a Novel Class of DNA Polymerase and Gyrase Inhibitors for MRSA Eradication in Cutaneous Infection
Yang, Frontiers in microbiology 2019 - “...of DNA into RNA using the four ribonucleoside triphosphates as substrates. 3 Aconitate hydratase A P63434 99.135 22 120(34%) 0.698 1.245 Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA) (acetyl degradation route) and probably the 2-methylcitrate cycle 1 (propionate...”
- Citrate serves as a signal molecule to modulate carbon metabolism and iron homeostasis in Staphylococcus aureus
Chen, PLoS pathogens 2024 - “...), fhuABG ( SA0602-SA0604 ), feoAB ( SA2338-SA2337 ), efeOBU ( SA0331-SA0333 ), citB ( SA1184 ), yfmCDE ( SA1979-SA1977 ), trxB ( SA2162 ), tatAC ( SA0335-SA0334 ), sstABCD (S A0688-SA0691 ), sirABC ( SA0111-SA0109 ), sbnABCDEFGHI ( SA0112-SA0120 ), sfnaABC ( SA1982-SA1980 ), ntrA...”
- Respiration and Small Colony Variants of Staphylococcus aureus
Proctor, Microbiology spectrum 2019 (secret) - The effect of skin fatty acids on Staphylococcus aureus
Neumann, Archives of microbiology 2015 - “...Transketolase 1.31 201 SA1177 Tkt Transketolase 3.11 401 SA1533 AckA Acetate kinase homologue 0.40 393 SA1184 CitB (AcnA) Aconitate hydratase 0.23 128 SA1216 PepF Hypothetical protein, similar to oligoendopeptidase 33.50 215 SA1283 Pbp2 PBP2 0.57 220 SA1308 RpsA 30S ribosomal protein S1 0.43 363 SA1336 Glucose-6-phosphate...”
- “...phosphatase 0.18 244 SA1100 Tsf Elongation factor Ts 0.47 470 SA1177 Tkt Transketolase 0.25 201 SA1184 CitB (acnA) Aconitate hydratase 0.13 128 SA1409 Dnak Molecular chaperone DnaK 0.19 226 SA1627 SplF Serine protease SplE, putative 0.36 670 SA1630 SplB Serine protease SplB 0.29 646 SA1631 SplA...”
- Exposure of clinical MRSA heterogeneous strains to β-lactams redirects metabolism to optimize energy production through the TCA cycle
Keaton, PloS one 2013 - “...pckA 5.6 Phosphoenol-pyruvate carboxykinase Involved in the TCA cycle and pyruvate metabolism (catalyzesATP+oxaloacetate=ADP+phosphoenolpyruvate+CO 2 ) SA1184 citB 6.3 aconitate hydratase Involved in the TCA cycle (conversion of citrate to isocitrate). SA1244 odhB 2.2 Dihydrolipo-amide acetyltrans-ferase Involved in the TCA cycle and lysine degradation. SA1518 citZ 8.9...”
- Single-nucleotide polymorphism genotyping identifies a locally endemic clone of methicillin-resistant Staphylococcus aureus
Nübel, PloS one 2012 - “...ST225_au133-1 T A synonymous 378156 SA0321 carbohydrate kinase, PfkB family ST225_au345-1 T C non-synonymous 1351084 SA1184 aconitate hydratase ST225_au131-1 G T intergenic 91487 none non-coding 10.1371/journal.pone.0032698.t002 Table 2 PCR Primers and probes. SNP Primers Probes 133-1-T/A 5-GCAATTGCAGTGGCTGTG-3 FAM- 5-AATGATGGCATAGTCTA-3 -MGB 5-CCCGAATTGTTCAAATTGTTTT-3 VIC- 5-ATGATGGCATTGTCTA-3 -MGB 345-1-T/C 5-TGGTAAAGGTAATGACGGTGAA-3...”
- Serine/threonine phosphatase Stp1 contributes to reduced susceptibility to vancomycin and virulence in Staphylococcus aureus
Cameron, The Journal of infectious diseases 2012 - “...SA2186 nasF Uroporphyrin-III C-methyl transferase 2.16 SA1082 SA1184 rimM citB 16S ribosomal RNA processing protein Aconitate hydratase 1.69 1.62 SA2180 nreB...”
- Transcriptional profiling analysis of the global regulator NorG, a GntR-like protein of Staphylococcus aureus
Truong-Bolduc, Journal of bacteriology 2011 - “...transport and metabolism SA0175 SA0516 SA1280 SA0871 SA0931 SA1184 SA1606 SA1607 SA1913 SA2135 SA0135 SA0237 SA0478 SA0517 SA1173 SA2553 SA1731 SA0172 SA0118...”
- Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism
Bohn, Nucleic acids research 2010 - “...SuccinylCoA synthetase subunit beta SA1089 ( sucD ) 0.47 f 15.51 SuccinylCoA synthetase subunit alpha SA1184 ( citB ) 0.47 15.61 Aconitate hydratase SA1365 ( gcvPB ) 0.48 Glycine dehydrogenase subunit 2 SA1366 ( gcvPA ) 0.36 0.48 21.88 Glycine dehydrogenase subunit 1 SA1367 ( gcvT...”
- “...ydro f olate (THF) required for the one-carbon shuffle, and (ii) SA1088-SA1089 ( sucC-sucD ), SA1184 ( citB ) and SA1517-SA1518 ( citC-citZ ) mRNA, which produce enzymes of the Krebs cycle. The growth inhibitory effect of RsaE overproduction is consistent with the observed downregulation of...”
- More
- The SAV1322 gene from Staphylococcus aureus: genomic and proteomic approaches to identification and characterization of gene function
Kim, BMC microbiology 2016 - “...protease ATP-binding subunit ClpC Stress response 1.72 SAV0975 clpB ClpB chaperone homologue Stress response 2.13 SAV1350 citB Aconitate hydratase Tricarboxylic acid cycle 1.55 SAV1579 dnaJ Chaperone protein DnaJ Stress response 1.56 SAV1580 dnaK Chaperone protein DnaK Stress response 2.30 SAV1581 grpE Heat shock protein GrpE Stress...”
- Influence of iron and aeration on Staphylococcus aureus growth, metabolism, and transcription
Ledala, Journal of bacteriology 2014 - “...RTF sstC RTR SAVrRNA16 SAVrRNA16 SAV1350 SAV1350 SAV2584 SAV2584 SAV0116 SAV0116 SAV0735 SAV0735 CGTGCTACAATGGACAATACAAA ATCTACGATTACTAGCGATTCCA...”
- Exploring the targetome of IsrR, an iron-regulated sRNA controlling the synthesis of iron-containing proteins in Staphylococcus aureus
Ganske, Frontiers in microbiology 2024 - “...change pJLisrR/pJLctrl ( q -value) IsrR-binding predicted in RBS region Negatively regulated by IsrR CitB (SAOUHSC_01347) exp: 2.5 (3.9e39) stat: 4.9 (4.6e50) exp: 3.5 (4.2e16) stat: 4.6 (6.9e50) x SucA (SAOUHSC_01418) exp: 2.1 (6.1e12) stat: 3.5 (4.0e51) exp: 2.1 (6.6e08) stat: 1.8 (5.6e28) KatA (SAOUHSC_01327) exp:...”
- The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus
Rochat, Nucleic acids research 2018 - “...sucC Succinyl-CoA synthetase, beta subunit 2.69 0.64 SAOUHSC_01218 sucD Succinyl-CoA synthetase, alpha subunit 2.40 0.63 SAOUHSC_01347 citB Aconitate hydratase 1 1.82 0.38 SAOUHSC_01416 sucB 2-oxoglutarate dehydrogenase, E2 component 1.35 0.53 SAOUHSC_01418 sucA 2-oxoglutarate dehydrogenase, E1 component 1.36 0.48 SAOUHSC_01983 fumC Fumarate hydratase 1.49 0.42 Malate metabolism...”
- Advantage of upregulation of succinate dehydrogenase in Staphylococcus aureus biofilms
Gaupp, Journal of bacteriology 2010 - “...synthase (SAOUHSC_01802); CitB, aconitate hydratase (SAOUHSC_01347); CitC, isocitrate dehyrogenase (SAOUHSC_01801); OdhA/ Kgd, 2-oxoglutarate dehydrogenase E1...”
- Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
Pagels, Molecular microbiology 2010 - “...(0.2) 0.4 (0.3) 1.3 (0.7) 0.3 (0.5) 1 (0.3) SACOL0987 3-oxoacyl-(acyl carrier protein) synthase III SAOUHSC_01347 0.2 (0.4) 1.4 (0.6) 0.5 (0.9) 2.7 (0.6) 0.4 (0.4) SACOL1385 Aconitate hydratase SAOUHSC_01968 0.3 (0.3) 0.3 (0.5) 0.4 (0.3) 0.9 (1.5) 0.9 (0.9) SACOL1894 HIT family protein SAOUHSC_02244 0.6...”
- Citrate serves as a signal molecule to modulate carbon metabolism and iron homeostasis in Staphylococcus aureus
Chen, PLoS pathogens 2024 - “...The transposon was inserted 591 bp downstream the translational start site of citB (locus tag: SAUSA300_1246). For JE2 Tn srtB , the transposon was inserted 260 bp downstream the translational start site of srtB (locus tag: SAUSA300_1034). Newman Tn citB and Newman Tn srtB mutant were...”
- Human Urine Alters Methicillin-Resistant Staphylococcus aureus Virulence and Transcriptome
Paudel, Applied and environmental microbiology 2021 (secret) - Growth and Stress Tolerance Comprise Independent Metabolic Strategies Critical for Staphylococcus aureus Infection
Kim, mBio 2021 - “...8230 9634 975 + + + + SAUSA300_2058 atpD 15858 20061 918 + + + SAUSA300_1246 acnA 260115 ** 226134 * 9414 + + + + SAUSA300_2003 rimIispAispA 6035 1611 1003 + + + SAUSA300_1098 pyrE 5427 5622 986 + + + + SAUSA300_1518 cshB 7221...”
- “...109.2535.93 105.3740.85 40.678.63 0.840.29 ** SAUSA300_1641 gltA 7.5 1.090.03 ** 3.23 108.524.61 119.6325.29 35.076.38 0.60.21 SAUSA300_1246 acnA 4.8 1.020.04 2.51 77.0834.94 * 85.6320.98 29.829.97 0.760.31 * SAUSA300_1014 pyc 5.54 0.830.04 **** 2.85 66.5318.67 **** 145.2136.90 * 24.096.32 *** 0.640.32 SAUSA300_1902 PPP 5.98 0.940.12 *** 3.84 40.5811.60...”
- Enzymes Catalyzing the TCA- and Urea Cycle Influence the Matrix Composition of Biofilms Formed by Methicillin-Resistant Staphylococcus aureus USA300
De, Microorganisms 2018 - “...repository NE861 (NR-47404) JE2 Tn mutant (insertion position: 1367722) in aconitate hydratase ( acnA , SAUSA300_1246; 1367131-1369836). NARSA repository Plasmids pALC2073 Contains the pSK236 vector, with the tetR -gene and the xyl / tetO promotor, originating from pWH35. [ 22 ] pGV5990 argH gene amplified with...”
- The Suf Iron-Sulfur Cluster Biosynthetic System Is Essential in Staphylococcus aureus, and Decreased Suf Function Results in Global Metabolic Defects and Reduced Survival in Human Neutrophils
Roberts, Infection and immunity 2017 - “...Genotype USA300_LAC sigB (SAUSA300_2022) acnA::tetM (SAUSA300_1246) nfu (SAUSA300_0839) kat::Tn (ermB) (SAUSA300_1232) nth::Tn (ermB) (SAUSA300_1343) mutY::Tn...”
- Staphylococcus aureus SufT: an essential iron-sulphur cluster assembly factor in cells experiencing a high-demand for lipoic acid
Mashruwala, Molecular microbiology 2016 - “...fur::tetM ( Torres et al. , 2010 ) JMB2475 acnA :: TN ( ermR ) (SAUSA300_1246) NARSA ( Fey et al. , 2013 ) JMB3539 acnA :: TN ( ermR ) sufT This study JMB5674 clpC :: TN ( ermR ) (SAUSA300_0510) NARSA ( Fey et...”
- “...clpC overexpression; this work pEPSA5_ clpC G672R SAUSA300_0510 G672R clpC overexpression; this work pEPSA5_ acnA SAUSA300_1246 acnA overexpression ( Mashruwala et al. , 2015 ) pEPSA5_ nfu SAUSA300_0839 nfu overexpression ( Mashruwala et al. , 2015 ) pEPSA5_ sufA SAUSA300_0839 sufA overexpression ( Rosario-Cruz et al....”
- Bacillithiol has a role in Fe-S cluster biogenesis in Staphylococcus aureus
Rosario-Cruz, Molecular microbiology 2015 - “...NE718 SAUSA300_2006( ilvD )::Tn( ermB ) JE2 ( Fey et al. , 2013 ) NE861 SAUSA300_1246( acnA )::Tn( ermB ) JE2 ( Fey et al. , 2013 ) NE1932 SASUA300_1513( sodA )::Tn( ermB ) JE2 ( Fey et al. , 2013 ) NE1224 SAUSA300_0135( sodM )::Tn(...”
- “...ermB ) LAC JMB4417 SAUSA300_1071( bshC ) , 2006( ilvD )::Tn( ermB ) LAC JMB3702 SAUSA300_1246( acnA )::Tn( ermB ) LAC JMB5225 SAUSA300_1349( bshA ) ::kanR , 1246( acnA )::Tn( ermB ) LAC JMB3703 SAUSA300_1071( bshC ), 1246( acnA )::Tn( ermB ) LAC JMB6203 SAUSA300_1513( sodA...”
- Nfu facilitates the maturation of iron-sulfur proteins and participates in virulence in Staphylococcus aureus
Mashruwala, Molecular microbiology 2015 - “...al. , 2001a ) JMB1163 acnA::tet LAC ( Sadykov et al. , 2010 ) NE861 SAUSA300_1246 (acnA)::TN(ermB) NARSA( Fey et al. , 2013 ) NE892 SAUSA300_2012 (leuC)::TN(ermB) NARSA( Fey et al. , 2013 ) NE805 SAUSA300_1178 ( recA ):: TN ( ermB ) NARSA( Fey et...”
- “...pEPSA5 Genetic complementation ( Forsyth et al. , 2002 ) pEPSA5_ nfu SAUSA300_0839 pEPSA5_ acnA SAUSA300_1246 pEPSA5_FLAG_ leuCD SAUSA300_2012-3 pEPSA5_ IlvD SAUSA300_2006 pEPSA5_ ahpCF SAUSA300_0379-80 pCM11 Transcriptional fusion containing promoterless gfp ( Malone et al. , 2009 ) pCM11_ acnA p SAUSA300_1246 promoter pCM11_ dps p...”
- Crizotinib Shows Antibacterial Activity against Gram-Positive Bacteria by Reducing ATP Production and Targeting the CTP Synthase PyrG
Zheng, Microbiology spectrum 2022 - “...( Fig.3E ). Interestingly, the DIA-based proteomics data showed that the upregulation of five proteins (NWMN_1263, CitC, SucB, SdhC, and CitZ) involved in the tricarboxylic acid (TCA) cycle and downregulation of two key proteins (PycA and IpdA) responsible for the conversion of pyruvate to acetyl coenzyme...”
- “...that the amount of intracellular acetyl-CoA significantly decreased in crizotinib-treated bacteria ( Fig.4B ). Meanwhile, NWMN_1263, CitC, SucB, SdhC, and CitZ, involved in the TCA cycle, were simultaneously increased in crizotinib-treated bacteria, which may need more ATP to resist the stimulation of drug. However, the ATP...”
- Transcriptional Response of Staphylococcus aureus to Sunlight in Oxic and Anoxic Conditions
McClary, Frontiers in microbiology 2018 - “...synthetase 0.38 8.6 gapR Glycolytic operon regulator 0.36 13.0 citC Isocitrate dehydrogenase, NADP-dependent 0.36 19.2 NWMN_1263 Aconitate hydratase 0.35 21.7 NWMN_0845 ATP-dependent Clp protease, ATP-binding subunit ClpB 0.34 5.7 NWMN_1529 ATPase AAA family protein 0.34 6.4 NWMN_2210 Formate dehydrogenase homolog 0.31 4.4 sdhA Succinate dehydrogenase flavoprotein...”
- Global analysis of the Staphylococcus aureus response to mupirocin
Reiss, Antimicrobial agents and chemotherapy 2012 - “...Functiona 10 min 30 min 60 min c2 SACOL1385 SACOL1673 SACOL1984 SACOL2097 AcnA AlaS AldA2 AtpA Aconitate hydratase Alanyl-tRNA synthetase Aldehyde dehydrogenase...”
- Redox sensing by a Rex-family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus
Pagels, Molecular microbiology 2010 - “...carrier protein) synthase III SAOUHSC_01347 0.2 (0.4) 1.4 (0.6) 0.5 (0.9) 2.7 (0.6) 0.4 (0.4) SACOL1385 Aconitate hydratase SAOUHSC_01968 0.3 (0.3) 0.3 (0.5) 0.4 (0.3) 0.9 (1.5) 0.9 (0.9) SACOL1894 HIT family protein SAOUHSC_02244 0.6 (0.6) 0.3 (0.4) 0.7 (0.4) 0.4 (1) 0.9 (1.4) SAOUHSC_02441 Asp23...”
- Complete and SOS-mediated response of Staphylococcus aureus to the antibiotic ciprofloxacin
Cirz, Journal of bacteriology 2007 - “...SA1245 SACOL1158 SACOL1159 SACOL1160 SACOL1262 SACOL1263 SACOL1385 SACOL1448 SACOL1449 SACOL1741 SACOL1908 SACOL2424 SACOL2426 SACOL2427 SACOL2428 Gene S....”
- Understanding the Mechanism of Action of NAI-112, a Lanthipeptide with Potent Antinociceptive Activity
Tocchetti, Molecules (Basel, Switzerland) 2021 - “...to SAFDA_0508 SNP C to A b Non-coding Poly(glycerol-phosphate) alpha-glucosyltransferase GTP cyclohydrolase 1340002 SAFDA_1245 to SAFDA_1246 SNP G to A Non-coding Glycine betaine transporteraconitate hydratase 1357466 SAFDA_1256 SNP T to C b Synonymous Transposase 1721972 SAFDA_1584 SNP A to G Synonymous Truncated transposase 1721999 SNP T...”
Hlac_3040 aconitate hydratase 1 from Halorubrum lacusprofundi ATCC 49239
51% identity, 97% coverage
- Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi
Liao, Scientific reports 2016 - “...including: acetyl-CoA synthesis (Hlac_1306, Hlac_0890, Hlac_0891, Hlac_0967); the utilization of acetyl-CoA via the glyoxylate cycle (Hlac_3040; Hlac_2153); the carboxylation of phosphoenolpyruvate (PEP) to oxaloacetate (Hlac_2311); the conversion of glycerol into dihydroxyacetone phosphate (DHAP) (Hlac_2109, Hlac_1458, Hlac_1122, Hlac_1124); and purine synthesis (Hlac_1295, Hlac_1250). Proteins associated with responses...”
- “...isocitrate lyase (AceA) 3.9 ns 2.5 ns Hlac_2311 phosphoenolpyruvate carboxylase (Ppc) 1.5 ns 2.0 ns Hlac_3040 aconitate hydratase 1.3 ns 1.7 ns One carbon metabolism Hlac_1238 formate dehydrogenase, alpha subunit (FdhA) 1.5 2.0 Carbohydrate metabolism Hlac_1300 gluconate dehydratase (GnaD) 1.6 ns 2.3 ns Hlac_1672 glyceraldehyde-3-phosphate dehydrogenase,...”
DA471_RS02880 aconitate hydratase AcnA from Staphylococcus aureus
49% identity, 98% coverage
Q6GH55 Aconitate hydratase A from Staphylococcus aureus (strain MRSA252)
49% identity, 98% coverage
- Multi-Omics Analysis Reveals Anti-Staphylococcus aureus Activity of Actinomycin D Originating from Streptomyces parvulus
Lin, International journal of molecular sciences 2021 - “...by analyzing the ROS levels under the treatment of actinomycin D. Otherwise, aconitate hydratase A (Q6GH55; AH), related to the TCA cycle, influenced the level of free fatty acids. It is known that free fatty acids provide energy for the uncoupling of respiration and phosphorylation in...”
- “...]. Enzymes related to the TCA cycle and pyruvate metabolism, such as aconitate hydratase A (Q6GH55; ACNA), 2-oxoglutarate dehydrogenase E1 component (Q6GGZ5; ODHA), and malate: quinone oxidoreductase 1 (Q6GE66; MQO1), were all down-regulated in the actinomycin D treatment group. On the one hand, ACNA, related to...”
- Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials
Hiltunen, Microorganisms 2019 - “...Pyruvate dehydrogenase E1PDHB Q6GHZ1 Triosephosphate isomeraseTPI Q6GIL6 ATP-dependent 6-phosphofructokinasePFK Q6GG08 2,3-phosphoglycerate mutasePPGM Q6GE17 Aconitase AAcnA Q6GH55 L-lactate dehydrogenase 1L-LDH Q6GK73 D-lactate dehydrogenaseD-LDH Q6GDS2 Alkaline shock protein 23Asp23 Q6GEP7 Alcohol dehydrogenaseADH Q99W07 Trigger factorTf Q6GG30 DNA-directed RNA polymeraseRpoB Q6GJC5 Alkyl hydroperoxide reductaseAhpC Q6GJR7 Alkyl hydroperoxide reductaseAhpF Q6GJR8...”
ACNA_CORGL / Q8NQ98 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see 5 papers)
NCgl1482 aconitate hydratase from Corynebacterium glutamicum ATCC 13032
47% identity, 98% coverage
- function: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate (PubMed:20647021, PubMed:21984793). Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2- methylisocitrate. The apo form of AcnA functions as a RNA-binding regulatory protein (By similarity).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
catalytic activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis- aconitate + H2O (RHEA:17941)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer.
disruption phenotype: Cells lacking this gene are glutamate auxotrophic in glucose minimal medium, show a strong growth defect, and secret large amounts of acetate. Deletion causes a strong selection pressure for secondary mutations within the gltA gene, which might be caused by a growth-inhibitory level of cytoplasmic citrate. - Accelerated Growth of Corynebacterium glutamicum by Up-Regulating Stress- Responsive Genes Based on Transcriptome Analysis of a Fast-Doubling Evolved Strain
Park, Journal of microbiology and biotechnology 2020 - “...synthase 0.73 NCgl0967 fum Fumarase 1.44 NCgl1084 odhA(kgd) OGDHC (oxoglutarate dehydrogenase complex) E1o subunit 0.99 NCgl1482 acn Aconitase 3.95 NCgl1523 ppc PEP Carboxylase 0.72 NCgl1926 mqo Malate: quinone oxidoreductase 5.18 NCgl2008 pyk Pyruvate kinase 0.90 Ncgl2126 sucB(aceF) OGDHC E2o subunit 1.47 NCgl2476 sucD OGDHC E2o subunit...”
- Recent Advances of L-ornithine Biosynthesis in Metabolically Engineered Corynebacterium glutamicum
Wu, Frontiers in bioengineering and biotechnology 2019 - “...pathway was also modulated by overexpressing the glt, icdh ( NCgl0634 ), and ach ( NCgl1482 ) genes in the L-ornithine-producing C. glutamicum SO16 strain. The insertion of a strong P sod promoter in the upstream region of glt improved the yield of L-ornithine from 30.8...”
- Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect L-Lysine Production in Corynebacterium glutamicum
Kim, International journal of molecular sciences 2016 - “...phosphoenolpyruvate carboxylase), which was downregulated, the other 9 genes (NCgl2167; aceE , NCgl0355; lpd , NCgl1482; acn , NCgl0634; icd , NCgl1084; kgd , NCgl2297; mdh , NCgl2765; pck , NCgl2008; pyk , and NCgl2521; pqo ) were upregulated ( Table S4 ). Using the Blast2GO...”
- Altered acetylation and succinylation profiles in Corynebacterium glutamicum in response to conditions inducing glutamate overproduction
Mizuno, MicrobiologyOpen 2016 - “...AceE, pyruvate dehydrogenase subunit E1 (NCgl2167); GltA, type II citrate synthase (NCgl0795); Acn, aconitate hydratase (NCgl1482); Icd, monomeric isocitrate dehydrogenase (NADP + ) (NCgl0634); Kgd, alphaketoglutarate decarboxylase (NCgl1084); AceF, dihydrolipoamide acetyltransferase (NCgl2126); Lpd, dihydrolipoamide dehydrogenase (NCgl0355); SdhA, succinate dehydrogenase flavoprotein subunit (NCgl0360); SdhB, succinate dehydrogenase ironsulfur...”
- Two-component systems of Corynebacterium glutamicum: deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response
Kocan, Journal of bacteriology 2006 - “...cg2777 cg2782 cg2843 cg2844 cg2845 cg2846 NCgl1444 NCgl1482 NCgl1521 NCgl1646 NCgl1859 NCgl1861 NCgl1915 NCgl1917 NCgl1959 NCgl2001 NCgl2127 NCgl2128 NCgl2393...”
- The DtxR regulon of Corynebacterium glutamicum
Wennerhold, Journal of bacteriology 2006 - “...NCgl0361 NCgl1141 NCgl1142 NCgl1143 NCgl1262 NCgl1263 NCgl1482 NCgl2319 NCgl2657 Catalase, katA Succinate dehydrogenase, sdhC Succinate dehydrogenase, sdhA...”
- Adaptation of Corynebacterium glutamicum to ammonium limitation: a global analysis using transcriptome and proteome techniques
Silberbach, Applied and environmental microbiology 2005 - “...NCgl0817 NCgl1161 NCgl1162 NCgl1163 NCgl1164 NCgl1165 NCgl1166 NCgl1482 NCgl1512 NCgl2579 NCgl2904 cg0442 cg0973 cg1364 cg1365 cg1366 cg1367 cg1368 cg1369...”
- “...NCgl2521 NCgl1526 NCgl1162 NCgl2167 NCgl2167 NCgl2167 NCgl1482 NCgl0935 NCgl2673 NCgl2297 NCgl2521 NCgl1526 NCgl0795 NCgl2008 NCgl0954 NCgl0666 NCgl2582...”
- Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine
Lange, Applied and environmental microbiology 2003 - “...2096b 2280b 2281b 2425 2453b NCgl1918 NCgl2086 (2274263-2274442) NCgl1482 NCgl1502 2455b NCgl1503 b 2456 NCgl1504 2736 2790b 2791b 2792b 2793b NCgl1263 NCgl1224...”
cg1737 aconitate hydratase from Corynebacterium glutamicum ATCC 13032
47% identity, 98% coverage
- A pseudokinase version of the histidine kinase ChrS promotes high heme tolerance of Corynebacterium glutamicum
Krüger, Frontiers in microbiology 2022 - “...reductase 2, alpha subunit 0.30 0.00 cg1487 leuC , isopropylmalate isomerase large subunit 0.29 0.01 cg1737 acn , aconitate hydratase 0.29 0.01 cg2636 catA1 , catechol 1,2-dioxygenase 0.03 0.00 cg3048 pta , phosphate acetyltransferase 0.24 0.00 a Expression of selected genes given as the mRNA ratio...”
- CRISPRi-Library-Guided Target Identification for Engineering Carotenoid Production by Corynebacterium glutamicum
Göttl, Microorganisms 2021 - “...plasmid carrying the ackA (cg3047) sgRNA This work pS_dCas9_ acn pS_dCas9 plasmid carrying the acn (cg1737) sgRNA This work pS_dCas9_ crtB pS_dCas9 plasmid carrying the crtB (cg0721) sgRNA This work pS_dCas9_ crtE pS_dCas9 plasmid carrying the crtE (cg0723) sgRNA This work pS_dCas9_ crtEb pS_dCas9 plasmid carrying...”
- The Iron Deficiency Response of Corynebacterium glutamicum and a Link to Thiamine Biosynthesis
Küberl, Applied and environmental microbiology 2020 (secret) - Comprehensive Analysis of C. glutamicum Anaplerotic Deletion Mutants Under Defined d-Glucose Conditions
Kappelmann, Frontiers in bioengineering and biotechnology 2020 - “...CAF19365 cg0762 prpC2 2-methylcitrate synthase 6.49 4.73e-18 CAF19364 cg0760 prpB2 2-methylcitrate lyase 5.05 1.07e-21 CAF21548 cg1737 acn Aconitase 3.86 8.53e-37 CAF20774 cg3047 ackA Acetate kinase 3.75 7.39e-28 CAF21597 cg1792 whiA Putative transcriptional regulator-WhiA homolog 3.75 9.38e-06 CAF20775 cg3048 pta Phosphate acetyltransferase 3.43 1.42e-16 CAF19363 cg0759 prpD2...”
- “...in the coding region for ICD. Moreover, ICL (cg2560), MS (cg2559), and cis -aconitase (ACN, cg1737) are highly up-regulated in C. glutamicum ppc pyc ( Figure 3 and Table 3 ) and only in this mutant intracellular and extracellular accumulation of glyoxylate was detected ( Figure...”
- The pupylation machinery is involved in iron homeostasis by targeting the iron storage protein ferritin
Küberl, Proceedings of the National Academy of Sciences of the United States of America 2016 - “...cg0446 cg0447 cg1341 cg1342 cg1343 cg1344 cg1345 cg1487 cg1488 cg1737 cg2636 cg3047 cg3048 katA sdhC sdhA sdhB narI narJ narH narG narK leuC leuD acn catA ackA...”
- Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB
Sorger-Herrmann, BMC microbiology 2015 - “...phosphoenolpyruvate carboxykinase 0.22 cg3048 pta , phosphoacetyltransferase 0.27 cg2887 phoR , phosphate response regulator 0.33 cg1737 acn , aconitase 0.34 cg0949 gltA , citrate synthase 0.46 cg2406 ctaE , cytochrome aa 3 oxidase, subunit 0.47 cg2888 phoS , phosphate sensor kinase 0.47 cg2843 pstB , P...”
- Deletion of the aconitase gene in Corynebacterium glutamicum causes strong selection pressure for secondary mutations inactivating citrate synthase
Baumgart, Journal of bacteriology 2011 - “...contains only a single aconitase gene, named acn (cg1737). No other enzyme that can metabolize citrate, such as citrate lyase or ATP-citrate lyase, currently...”
- “...the role of aconitase in Corynebacterium glutamicum, acn (cg1737) in-frame deletion mutants were constructed and verified by colony PCR as described in...”
- Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis
Follmann, BMC genomics 2009 - “...dependent methyltransferase 0 n.d. 1.72 - - - - - - - - - 21 cg1737 acn Aconitase A 0 -0.58 1.66 4.4 5.3 6.2 4.4 5.0 4.9 2.2 2.6 2.5 RipA, RamA*, RamB* 22 cg1759 x sufX Predicted metal-sulfur cluster biosynthetic enzyme 0 0.94 1.34...”
- “...found RipA-regulated genes encoding iron containing enzymes like succinate dehydrogenase ( cg0446-0447 ), aconitase ( cg1737 ), or catalase ( cg0310 ) to be (slightly) repressed at pH 6, whereas the same genes were found to be induced at alkaline pH (Table 1 ). Additionally, higher...”
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LINJ_18_0510 putative aconitase from Leishmania infantum JPCM5
49% identity, 99% coverage
T303_07285 aconitate hydratase AcnA from Streptococcus thermophilus ASCC 1275
49% identity, 98% coverage
- Functional Genomic Analyses of Exopolysaccharide-Producing Streptococcus thermophilus ASCC 1275 in Response to Milk Fermentation Conditions
Wu, Frontiers in microbiology 2019 - “...helicase T303_04855 0.59 GTP-binding protein T303_02740 0.34 NrdR family transcriptional regulator T303_08865 0.49 Aminoglycoside phosphotransferase T303_07285 0.56 Aconitate hydratase T303_07275 0.63 Isocitrate dehydrogenase T303_01900 2.59 Hypothetical protein T303_04110 4.02 Branched-chain amino acid aminotransferase T303_08745 2.85 Copper-binding protein T303_04795 2.24 Cytoplasmic membrane protein T303_01820 0.35 Hypothetical protein...”
- Transcriptomic Insights Into the Growth Phase- and Sugar-Associated Changes in the Exopolysaccharide Production of a High EPS-Producing Streptococcus thermophilus ASCC 1275
Padmanabhan, Frontiers in microbiology 2018 - “...highly expressed at 5h in M17-L. In L10h, genes involved in citrate cycle (T303_07275, T303_07280, T303_07285), trehalose metabolism (T303_00355, T303_00360, T303_00365) and acetyl CoA carboxylase biotin carrier protein and subunits (T303_03105 T303_03115, T303_03120, T303_03125) were significantly upregulated. FIGURE 7 Changes in expression of genes associated with...”
D9QAH4 Aconitate hydratase from Corynebacterium pseudotuberculosis (strain C231)
48% identity, 95% coverage
- Changes in protein abundance are observed in bacterial isolates from a natural host
Rees, Frontiers in cellular and infection microbiology 2015 - “...CpC231_1066 SPFH domain, band seven integral membrane protein 0.075 1.213 * 1.581 * Posttranslational modification D9QAH4 acnA CpC231_1074 Aconitate hydratase 0.665 1.274 * 1.321 Energy production and conversion D9QAQ7 CpC231_1157 CpC231_1157 Citrate lyase subunit beta-like protein 1.008 * 1.593 * 1.843 * Carbohydrate transport and metabolism...”
HVO_0541 aconitate hydratase 1 from Haloferax volcanii DS2
50% identity, 98% coverage
- A comprehensive analysis of the importance of translation initiation factors for Haloferax volcanii applying deletion and conditional depletion mutants
Gäbel, PloS one 2013 - “...sRNA45 2.35 0,13 6 sRNA45 2.28 0,11 6 sRNA45 2.27 0,11 4 437-F11 citB1, acnA HVO_0541 2.18 0,12 3 441-D11 capB HVO_B0153 2.16 0,14 4 433-G05 top6A HVO_1570 0.48 0,50 6 439-A06 atpF, atpA HVO_0315-0316 0.46 0,31 6 435-D08 rpoB1 HVO_0348 0.46 0,58 6 432-B01 HVO_1290...”
MAB_2730 Probable aconitate hydratase ACN from Mycobacterium abscessus ATCC 19977
49% identity, 96% coverage
CpC231_1074 aconitate hydratase AcnA from Corynebacterium pseudotuberculosis C231
48% identity, 95% coverage
- Changes in protein abundance are observed in bacterial isolates from a natural host
Rees, Frontiers in cellular and infection microbiology 2015 - “...domain, band seven integral membrane protein 0.075 1.213 * 1.581 * Posttranslational modification D9QAH4 acnA CpC231_1074 Aconitate hydratase 0.665 1.274 * 1.321 Energy production and conversion D9QAQ7 CpC231_1157 CpC231_1157 Citrate lyase subunit beta-like protein 1.008 * 1.593 * 1.843 * Carbohydrate transport and metabolism D9QAQ8 CpC231_1158...”
XP_001016756 cytoplasmic-like aconitate hydratase from Tetrahymena thermophila SB210
49% identity, 98% coverage
ZP_02144175 aconitate hydratase from Phaeobacter gallaeciensis BS107
47% identity, 97% coverage
- Assessing the exoproteome of marine bacteria, lesson from a RTX-toxin abundantly secreted by Phaeobacter strain DSM 17395
Durighello, PloS one 2014 - “...21 9 ZP_02144479 Extracellular solute-binding protein, family 5 528 413 57617 17 7 20 10 ZP_02144175 Aconitate hydratase 895 431 96861 13 10 19 1 Detected with at least three different peptides. *Periplasmic component. De novo Sequencing with High Resolution Data Revealed the Identity of the...”
- “...ZP_02143869 phosphate ABC transporter * 37145 221 9 2 3 100 221 9 2 3 ZP_02144175 aconitate hydratase 96861 232 9 6 9 33 112 3 2 2 ZP_02147263 outer membrane porin 31831 213 20 5 10 40 115 7 2 5 ZP_02145456 Flagellar hook-associated protein...”
E6P09_05600 aconitate hydratase AcnA from Haloferax mediterranei ATCC 33500
50% identity, 97% coverage
E2566_12300 aconitate hydratase AcnA from Pectobacterium punjabense
50% identity, 97% coverage
GOX1335 Aconitate hydratase from Gluconobacter oxydans 621H
50% identity, 97% coverage
- Global mRNA decay and 23S rRNA fragmentation in Gluconobacter oxydans 621H
Kranz, BMC genomics 2018 - “...of the incomplete TCA cycle ( ) the transcript of aconitase of ( acn , GOX1335) was the most stable, while the transcripts of malate:quinone oxidoreductase ( mqo , GOX2070), dihydrolipoamide succinyl transferase (E2) of 2-oxoglutarate dehydrogenase ( odhB , GOX1073), and dihydrolipoamide dehydrogenase ( lpd...”
- “...apparent expression in the central metabolism based on the FPKM values, with the aconitase transcript (GOX1335) as the most stable (9.3min) above the global mean, and with the transcripts of odhB (GOX1073, 3.4min) encoding dihydrolipoamide succinyl transferase (E2) of the 2-oxoglutarate dehydrogenase, mqo (GOX2070, 4min) encoding...”
- Combined fluxomics and transcriptomics analysis of glucose catabolism via a partially cyclic pentose phosphate pathway in Gluconobacter oxydans 621H
Hanke, Applied and environmental microbiology 2013 - “...GOX1703 GOX1704 GOX1705 GOX0145 GOX1381 GOX1643 GOX0431 GOX1335 GOX1336 mRNA ratio of 45 genes, 2.0; 66 genes, 0.5 Triosephosphate isomerase Ribose-5-phosphate...”
- “...showed decreased mRNA ratios, aconitate hydratase (acn, GOX1335, ratio of 0.4), isocitrate dehydrogenase (icd, GOX1336, ratio of 0.3), and fumarate hydratase...”
FTN_1623 aconitate hydratase from Francisella tularensis subsp. novicida U112
50% identity, 97% coverage
Q5NII1 Aconitate hydratase from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
FTT_0087 aconitate hydratase from Francisella tularensis subsp. tularensis SCHU S4
50% identity, 97% coverage
- Shotgun Immunoproteomic Approach for the Discovery of Linear B-Cell Epitopes in Biothreat Agents Francisella tularensis and Burkholderia pseudomallei
D'haeseleer, Frontiers in immunology 2021 - “...GreA Q5NFC6 a IVGEDEADIDNQK 60 kDa chaperonin GroL Q5NEE1 a SFGTPTITK 2 Aconitate hydratase AcnA Q5NII1 a GIPLVILAGK 1 Chaperone protein DnaK Q5NFG7 a AYAEQAQAAVAQGGAK Chaperone protein DnaK Q5NFG7 a FHDLVTAR 2 Outer membrane protein 26 Omp26 Q5NES2 DGSVGWVK 1 3-oxoacyl-ACP reductase FabG Q5NF68 VALVTGASR 1...”
- “...DnaK Q5NFG7 a ALEDAGLSK 2 Enoyl-ACP reductase [NADH] FabI Q5NGQ3 i TLAASGISNFK Aconitate hydratase AcnA Q5NII1 a TAHTTTFEALAR Elongation factor Ts Tsf Q5NHX9 a LDVGEGIEK 1 The columns under scores indicate whether the peptide was over or underrepresented in each of the 8 experimental samples compared...”
- Immunoproteomic analysis of the human antibody response to natural tularemia infection with Type A or Type B strains or LVS vaccination
Fulton, International journal of medical microbiology : IJMM 2011 - “...of 2-oxoglutarate dehydrogenase complex - 8 7 11 8 [29, 30] [27, 38], [39], [40] FTT_0087 Aconitate hydratase 1 - 1 4 - [29], [30], [38] FTT_0137 Elongation factor Tu (EF-Tu) 1 - - 1 4 [25], [29],[38], [39], [40] FTT_1486c Hypothetical lipoprotein - - -...”
- The francisella tularensis proteome and its recognition by antibodies
Kilmury, Frontiers in microbiology 2010 - “...2007 ) FTT_0086 Hypothetical protein Mouse 2D-Western blot Unknown Twine et al. ( 2006b ) FTT_0087 Aconitate hydratase Human, type A; human, presumed type B; mouse 2D-Western blot Cytoplasmic Janovska et al. ( 2007a , b ), Twine et al. ( 2010 ) FTT_0101 Conserved membrane...”
SAV_2258 aconitate hydratase from Streptomyces avermitilis MA-4680
50% identity, 95% coverage
- SAV742, a Novel AraC-Family Regulator from Streptomyces avermitilis, Controls Avermectin Biosynthesis, Cell Growth and Development
Sun, Scientific reports 2016 - “...tricarboxylic acid (TCA) cycle: aceE1 (sav_5800 , encoding a putative pyruvate dehydrogenase E1 component), acnA (sav_2258 , encoding a putative aconitase), korA (sav_4877 , encoding the alpha subunit of 2-oxoglutarate ferredoxin oxidoreductase) and icdA (sav_7214 , encoding a putative isocitrate dehydrogenase); in glycolysis: pgmA (sav_803 ,...”
FTH_1708 aconitate hydratase from Francisella tularensis subsp. holarctica OSU18
Q2A1K3 Aconitate hydratase A from Francisella tularensis subsp. holarctica (strain LVS)
FTL_1772 aconitate hydratase from Francisella tularensis subsp. holarctica
50% identity, 97% coverage
- Use of magnetic hydrazide-modified polymer microspheres for enrichment of Francisella tularensis glycoproteins
Horák, Soft matter 2012 - “...cyt FTH_1691 Elongation factor Tu 43.39/4.87 cyt cyt FTH_1696 Glycerol-3-phosphate dehydrogenase 57.78/8.36 ?, MLS cyt FTH_1708 Aconitate hydratase 102.70/5.30 cyt cyt FTH_1719 Dihydrolipoyllysine-residue succinyltransferase 52.75/4.89 cyt cyt FTH_1720 Oxoglutarate dehydrogenase (succinyl-transferring) 105.67/6.01 cyt cyt FTH_1721 Succinate dehydrogenase 27.2/8.42 CM cyt FTH_1722 Succinate dehydrogenase 65.86/6.14 CM cyt...”
- “...cyt FTH_1691 Elongation factor Tu 43.39/4.87 cyt cyt FTH_1696 Glycerol-3-phosphate dehydrogenase 57.78/8.36 ?, MLS cyt FTH_1708 Aconitate hydratase 102.70/5.30 cyt cyt FTH_1719 Dihydrolipoyllysine-residue succinyltransferase 52.75/4.89 cyt cyt FTH_1720 Oxoglutarate dehydrogenase (succinyl-transferring) 105.67/6.01 cyt cyt FTH_1721 Succinate dehydrogenase 26.57/8.18 CM cyt FTH_1722 Succinate dehydrogenase 65.86/6.14 CM cyt...”
- Multimethodological approach to identification of glycoproteins from the proteome of Francisella tularensis, an intracellular microorganism
Balonova, Journal of proteome research 2010 - “...dihydrolipoyllysine-residue succinyltransferase (FTH_1719), DNA-binding protein HU-beta (FTH_0880), 17 kDa lipoprotein TUL4 precursor (FTH_0414), aconitate hydratase (FTH_1708), cell division protein FtsZ (FTH_1830), LemA-like protein (FTH_0357), dihydrolipoamide dehydrogenase (FTH_0312), bacterioferritin (FTH_0620), thioredoxin family protein (FTH_1071), dihydrolipoamide acetyltransferase (FTH_0311), molecular chaperone DnaK (FTH_1167), and elongation factor Tu (FTH_1691) have...”
- “...- ? - FTH_0414 ConA, DSA, PNA, SBA, SNA 15.77 9.67 0 - ? SPII FTH_1708 ConA, DSA, PNA, SBA, SNA 102.70 5.30 4 - cyt - FTH_1721 ConA, DSA, PNA, SBA, SNA 26.57 8.18 1 - cyt - FTH_1503 ConA, DSA, PNA, SBA, SNA 69.85...”
- Possible links between stress defense and the tricarboxylic acid (TCA) cycle in Francisella pathogenesis
Dieppedale, Molecular & cellular proteomics : MCP 2013 - “...Q2A1M4 Q2A5I2 Q2A1G8 Q2A1J3 Q2A432 Q2A2S2 Q2A5B4 Q2A3T9 Q2A1M8 Q2A1K3 2284 Gene name FTL number Protein description Unique peptides mdh sucC tsf tig sucB tolB...”
- Further Characterization of the Capsule-Like Complex (CLC) Produced by Francisella tularensis Subspecies tularensis: Protective Efficacy and Similarity to Outer Membrane Vesicles
Champion, Frontiers in cellular and infection microbiology 2018 - “...of the CLC along with 5 other prominent OMV proteins (FTL_1146, FTL_1743, and FTL_1592, FTL_1442, FTL_1772) (Pierson et al., 2011 ). Overall, of the 12 proteins identified in the CLC soluble fraction, 8 were also present in OMV (FTL_1474, FTL_1907, FTL_0014, and FTL_0227), and of the...”
- “...N ATP synthase alpha chain 46 55,502 5.20E-05 FTL_0311 S Dihydrolipoamide dehydrogenase 44 50,495 9.90E-05 FTL_1772 T20, S Aconitate hydratase 44 102,639 0.00028 FTL_1784 E 2-oxoglutarate dehydrogenase E1 component 39 105,613 0.00029 FTL_0739 N Glucose inhibited division protein A 36 69,721 0.00053 FTL_1022 N Coproporphyinogen III...”
- Francisella tularensis LVS surface and membrane proteins as targets of effective post-exposure immunization for tularemia
Chandler, Journal of proteome research 2015 - “...synthetase subunit beta (SucC) f FTL_1553 Cyto(8.96) M, 50 T 41 aconitate hydratase (AcnA) f FTL_1772 Cyto(9.97) M 21 , 37 , 50 citrate synthase (GltA) FTL_1789 Cyto(9.97) cell division protein FtsZ f FTL_1907 Cyto(9.12) M 21 , 49 30S ribosomal protein S1 (RpsA) f FTL_1912...”
- Possible links between stress defense and the tricarboxylic acid (TCA) cycle in Francisella pathogenesis
Dieppedale, Molecular & cellular proteomics : MCP 2013 - “...FTL_1784 FTL_0785 FTL_1309 FTL_0295 FTL_0894 FTL_1743 FTL_1772 Chaperone protein DnaK* Chaperone protein HtpG* 60 kDa chaperonin* Pyruvate dehydrogenase...”
SCO5999 aconitate hydratase from Streptomyces coelicolor A3(2)
50% identity, 95% coverage
- A Proteomic Analysis Indicates That Oxidative Stress Is the Common Feature Triggering Antibiotic Production in Streptomyces coelicolor and in the pptA Mutant of Streptomyces lividans
Lejeune, Frontiers in microbiology 2021 - “...than in Pi limitation (group II). It includes the citrate synthase SCO2736, the aconitate hydratase SCO5999, the -ketoglutarate dehydrogenase E1 component SCO528 that generates NADH, and the subunit (SCO4808) of the succinyl-CoA synthase that generates guanosine triphosphate (GTP). Cluster C contains six enzymes that have a...”
- Expression of genes of the Pho regulon is altered in Streptomyces coelicolor
Millan-Oropeza, Scientific reports 2020 - “...those of the upper part of the TCA, the citrate synthase SCO2736 and the aconitase SCO5999 as well as the NADP+ dependent malic enzyme SCO2951 47 , were more abundant in SL than in SC throughout growth on glucose and glycerol, to a lesser extent. This...”
- The transcriptional regulator TamR from Streptomyces coelicolor controls a key step in central metabolism during oxidative stress
Huang, Molecular microbiology 2013 (PubMed)- “...of the tam (SCO3132), tamR (SCO3133) and aconitase (SCO5999) genes. Since oxidative stress leads to disassembly of the [4Fe-4S] cluster that is essential for...”
- “...encode enzymes of the citric acid cycle, aconitase (SCO5999) and malate synthase (SCO6243). Notably, the TamR binding site was conserved in the promoters of...”
- A novel two-component system involved in the transition to secondary metabolism in Streptomyces coelicolor
Rozas, PloS one 2012 - “...* RpmJ, 50S ribosomal protein L36 SCO4956 2.02 2.31 SCO4956 Possible peptide methionine sulfoxide reductase SCO5999 2.63 2.75 SCO5999 Hypothetical protein Secretory proteins SCO6109 2.18 2.19 SCO6108-6109 Possible secreted hydrolase Other genes SCO4253 2.27 2.36 SCO4253-4251 Hypothetical protein *Transcriptional units potentially ppGpp regulated [10] . M28/wt...”
- Extracting regulator activity profiles by integration of de novo motifs and expression data: characterizing key regulators of nutrient depletion responses in Streptomyces coelicolor
Iqbal, Nucleic acids research 2012 - “...of the R regulon ( 39 ). The other four targets are SCO0499 (putative formyltransferase), SCO5999 (aconitase), SCO1926 (putative DNA-binding protein) and SCO1623 (conserved hypothetical protein). The Streptomyces aconitases belong to the Iron-Regulatory-Protein/AcnA family, while the aconitase AcnA of S. viridochromogenes possesses regulatory function within iron...”
- Rapid functional screening of Streptomyces coelicolor regulators by use of a pH indicator and application to the MarR-like regulator AbsC
Yang, Applied and environmental microbiology 2010 - “...promoter regions of genes encoding aconitase (SacA, SCO5999), citrate synthase (CitA, SCO2736) and the E1 Downloaded from http://aem.asm.org/ on February 12,...”
- Streptomyces coelicolor A3(2) lacks a genomic island present in the chromosome of Streptomyces lividans 66
Zhou, Applied and environmental microbiology 2004 - “...SCO5997, encoding an unknown phage-related secreted protein, and SCO5999, encoding a putative aconitase). DISCUSSION S. lividans ZX7, a derivative of strain ZX1...”
- “...insertion, SCO5997 on the left and SCO5998 (murA1) and SCO5999 on the right, are shown inside the AseI-B fragment. (Bottom) Relative positions of the 15-bp...”
ACNA_STRVT / D9X0I3 Aconitate hydratase A; ACN; Aconitase; EC 4.2.1.3 from Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) (see 5 papers)
50% identity, 95% coverage
- function: Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid (TCA) cycle (PubMed:10559181, PubMed:23116164). Aconitase activity is important for the initiation of morphological and physiological differentiation of S.viridochromogenes (PubMed:10559181, PubMed:23116164). In addition, the apo form of AcnA (lacking the [4Fe-4S] cluster) functions as a RNA- binding regulatory protein, which binds to iron responsive elements (IREs) located on the untranslated region of certain mRNAs, including recA and ftsZ (PubMed:23116164). Binding to IRE-like structures probably alters the target mRNA stability and regulates the protein amount (PubMed:23116164). The apo form plays a regulatory role in oxidative stress response (PubMed:23116164, PubMed:24498397).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
catalytic activity: citrate = cis-aconitate + H2O (RHEA:10228)
catalytic activity: cis-aconitate + H2O = D-threo-isocitrate (RHEA:22144)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
disruption phenotype: Mutant is unable to develop aerial mycelium and to sporulate, resulting in a bald phenotype (PubMed:10559181). Furthermore, the mutant does not produce the antibiotic phosphinothricin tripeptide (PTT), suggesting a defect in physiological differentiation (PubMed:10559181). It is also sensitive to oxidative and temperature stress (PubMed:23116164). Mutant cannot be complemented by pmi (PubMed:11472937).
ACOHC_CAEEL / Q23500 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Gex-3-interacting protein 22; EC 4.2.1.3 from Caenorhabditis elegans (see 2 papers)
Q23500 aconitate hydratase (EC 4.2.1.3) from Caenorhabditis elegans (see paper)
NP_509898 Cytoplasmic aconitate hydratase from Caenorhabditis elegans
48% identity, 95% coverage
- function: Catalyzes the isomerization of citrate to isocitrate via cis- aconitate (PubMed:12438312). Has probably no RNA-binding activity (PubMed:12438312).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Interacts with gex-3. - Involvement of the iron regulatory protein from Eisenia andrei earthworms in the regulation of cellular iron homeostasis
Procházková, PloS one 2014 - “...AAK39637 Manduca sexta IRP-1 100 70 65 69 CAB41634 Pacifastacus leniusculus IRP-1-like 100 63 70 NP_509898 Caenorhabditis elegans Aconitase 1 100 63 AFI44047 Eisenia andrei IRP 100 Tissue expression profile analysis of EaIRP Coelomocytes and various tissues, including the epidermis, seminal vesicles, pharynx, esophagus, crop, gizzard...”
- Cytosolic aconitase and ferritin are regulated by iron in Caenorhabditis elegans.
Gourley, The Journal of biological chemistry 2003 (PubMed)- GeneRIF: biochemical characterization and regulation
- The RNA-binding protein repertoire of Arabidopsis thaliana.
Marondedze, Scientific reports 2016 (no snippet) - Altered proteostasis in aging and heat shock response in C. elegans revealed by analysis of the global and de novo synthesized proteome
Liang, Cellular and molecular life sciences : CMLS 2014 - “...P10567 unc-15 Paramyosin 3 0.4 0.2 P02566 unc-54 Myosin-4 8 0.7 0.4 Other cellular components Q23500 aco-1 Aconitase 3 0.7 0.5 P27604 ahcy-1 S -adenosylhomocysteine hydrolase homolog 22 0.6 0.4 6 0.5 0.3 P27604 ahcy-1 S -adenosylhomocysteine hydrolase homolog 20 0.7 0.5 8 0.6 0.4 P54216...”
- The phosphinomethylmalate isomerase gene pmi, encoding an aconitase-like enzyme, is involved in the synthesis of phosphinothricin tripeptide in Streptomyces viridochromogenes.
Heinzelmann, Applied and environmental microbiology 2001
Q9RIL1 aconitate hydratase (EC 4.2.1.3) from Streptomyces viridochromogenes (see paper)
50% identity, 95% coverage
TP01_1050, TpMuguga_01g01050 aconitate hydratase 1 from Theileria parva strain Muguga
50% identity, 98% coverage
- Characterization of the Theileria parva sporozoite proteome
Nyagwange, International journal for parasitology 2018 - “...PK, pyruvate kinase (TpMuguga_02g00134, TpMuguga_04g00607); LDH, lactate dehydrogenase (TpMuguga_01g01182); PC, pyruvate carboxylase; ACH, aconitate hydratase-1 (TpMuguga_01g01050); IDH, isocitrate dehydrogenase (TpMuguga_04g00620); OGDH, oxoglutarate dehydrogenase (TpMuguga_01g00262); SCL, succinyl coenzyme A ligase (TpMuguga_04g00660); SDH, succinate dehydrogenase (TpMuguga_01g00210); FH, fumarate hydratase-1 (TpMuguga_03g00078); MDH, malate dehydrogenase; MQOR, Malate: quinone oxidoreductase (TpMuguga_03g00758);...”
- The 2-methylcitrate cycle is implicated in the detoxification of propionate in Toxoplasma gondii
Limenitakis, Molecular microbiology 2013 - “...ETH_00007195 PrpC NCLIV_024780 ETH_00026655 PrpD NCLIV_013410 ETH_00015105 PrpB NCLIV_000780 Contig 1606+713 Acn NCLIV_046260 ETH_00020300 PF13_0229 TP01_1050 NCLIV_033680 ETH_00021115 PF08_0031 cgd1_600 TP02_0450 Abbreviations Bb Pm Pt Tt PrpE XP_001460124 XP_001025576 PrpC XP_002787782 XP_001447471 XP_001023516 PrpD EER04808 XP_001457033 EAS06482 PrpB XP_002772756 XP_001459690 EAR89396 Acn BBOV_III011790 XP_002765934 XP_001451848 XP_001016756...”
ADL12_38700 aconitate hydratase AcnA from Streptomyces regalis
50% identity, 95% coverage
FHQ18_06070 aconitate hydratase AcnA from Deferribacter autotrophicus
47% identity, 95% coverage
- Genomic Insights into the Carbon and Energy Metabolism of a Thermophilic Deep-Sea Bacterium Deferribacter autotrophicus Revealed New Metabolic Traits in the Phylum Deferribacteres
Slobodkin, Genes 2019 - “...D. autotrophicus contains all the genes encoding TCA enzymes, including: citrate synthase (FHQ18_06115), aconitate hydratase (FHQ18_06070), isocitrate dehydrogenase (FHQ18_05945), 2-oxoglutarate/2-oxoacid ferredoxin oxidoreductase (FHQ18_01050, FHQ18_01055; FHQ18_05965, FHQ18_05970, FHQ18_05975, FHQ18_05980; FHQ18_06355, FHQ18_06360, FHQ18_06365, FHQ18_06370), succinyl-CoA ligase (FHQ18_05955, FHQ18_05960), succinate dehydrogenase (FHQ18_05990, FHQ18_05995), fumarate hydratase (FHQ18_05155), and malate dehydrogenase...”
Q0S0G5 Aconitate hydratase from Rhodococcus jostii (strain RHA1)
48% identity, 96% coverage
- Differential protein profiling of soil diazotroph Rhodococcus qingshengii S10107 towards low-temperature and nitrogen deficiency
Suyal, Scientific reports 2019 - “...(Phosphoribosylformylglycinamidine cyclo-ligase, Q0S760), Rha1_ro05098 (3-demethylubiquinone-9 3-O-methyltransferase, Q0S6F7), Rha1_ro06238, AroA, GltX2, MiaA, AroK, AcnA2 (Aconitate hydratase, Q0S0G5) and HemZ were under NB. CowN as a protein biomarker In order to identify the protein biomarker(s) associated with the nitrogen fixation process at lower temperatures, the author group has...”
XP_003357777 cytoplasmic aconitate hydratase from Sus scrofa
47% identity, 97% coverage
SSU1042 aconitate hydratase from Streptococcus suis P1/7
SSUSC84_RS05690 aconitate hydratase AcnA from Streptococcus suis SC84
48% identity, 97% coverage
DIP1283 aconitate hydratase from Corynebacterium diphtheriae NCTC 13129
47% identity, 95% coverage
PFCIRM121_08300, PFCIRM129_04640 aconitate hydratase AcnA from Propionibacterium freudenreichii
50% identity, 97% coverage
- Extracellular Vesicles Produced by the Probiotic Propionibacterium freudenreichii CIRM-BIA 129 Mitigate Inflammation by Modulating the NF-κB Pathway
Rodovalho, Frontiers in microbiology 2020 - “...identified as important actors in immunomodulation were packed within EVs: enolase (Eno1, PFCIRM129_06070), aconitase (Acn, PFCIRM129_04640), glutamine synthetase (GlnA1, PFCIRM129_11730), glucose-6-phosphate isomerase (Gpi, PFCIRM129_10645), triosephosphate isomerase (Tpi1, PFCIRM129_11290), the surface-layer proteins SlpB (PFCIRM129_00700) and SlpE (PFCIRM129_05460), the BopA solute binding protein (PFCIRM129_08120), internaline A (InlA, PFCIRM129_12235),...”
- “...interactions with the host. This is the case of enolase (Eno1, PFCIRM129_06070) and aconitase (Acn, PFCIRM129_04640) ( Deutsch et al., 2017 ). Others, such as glutamine synthetase (GlnA1, PFCIRM129_11730), glucose-6-phosphate isomerase (Gpi, PFCIRM129_10645) and triosephosphate isomerase (Tpi1, PFCIRM129_11290), have also been described as moonlighting proteins, with...”
- Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study
Deutsch, Scientific reports 2017 - “...methylase Yes PFCIRM129_07765 lacl1 C T4 (+) 0.693 Transcription regulation/transcriptional regulator, arabinose operon epressor No PFCIRM121_08300 acn C T5 () 0.683 TCA cycle/Aconitase No PFCIRM129_10930 pouf 10930 C G1 (+) 0.263 (+) 0.518 No function/No homology found in the databases No PFCIRM129_10925 pep C G2 (+)...”
SMU_RS03175 aconitate hydratase AcnA from Streptococcus mutans UA159
49% identity, 96% coverage
A1S_0558 aconitate hydratase 1 from Acinetobacter baumannii ATCC 17978
52% identity, 90% coverage
Q8VDC3 Cytoplasmic aconitate hydratase from Mus musculus
47% identity, 95% coverage
B9UNL8 Cytoplasmic aconitase/iron-regulatory protein from Ixodes ricinus
49% identity, 98% coverage
2b3xA / P21399 Structure of an orthorhombic crystal form of human cytosolic aconitase (irp1) (see paper)
47% identity, 95% coverage
- Ligand: iron/sulfur cluster (2b3xA)
ACO1 / P21399 cytoplasmic aconitate hydratase from Homo sapiens (see 6 papers)
ACOHC_HUMAN / P21399 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Homo sapiens (Human) (see 8 papers)
P21399 aconitate hydratase (EC 4.2.1.3) from Homo sapiens (see 3 papers)
NP_001265281 cytoplasmic aconitate hydratase from Homo sapiens
NP_002188 cytoplasmic aconitate hydratase from Homo sapiens
47% identity, 95% coverage
- function: Bifunctional iron sensor that switches between 2 activities depending on iron availability (PubMed:1281544, PubMed:1946430, PubMed:8041788). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization (PubMed:1281544, PubMed:1946430, PubMed:23891004, PubMed:8041788). Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed:1281544, PubMed:1946430, PubMed:23891004, PubMed:8041788).
function: Conversely, when cellular iron levels are high, binds a 4Fe- 4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis- aconitate.
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Interacts (when associated with the 4Fe-4S) with FBXL5. Interacts with frataxin(81-210). - Iron regulatory protein 1 is required for the propagation of inflammation in inflammatory bowel disease.
Fahoum, The Journal of biological chemistry 2024 - GeneRIF: Iron regulatory protein 1 is required for the propagation of inflammation in inflammatory bowel disease.
- ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.
Zhang, Nature cancer 2022 (PubMed)- GeneRIF: ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.
- Human IRP1 Translocates to the Nucleus in a Cell-Specific and Iron-Dependent Manner.
Gu, International journal of molecular sciences 2022 - GeneRIF: Human IRP1 Translocates to the Nucleus in a Cell-Specific and Iron-Dependent Manner.
- The Iron Maiden. Cytosolic Aconitase/IRP1 Conformational Transition in the Regulation of Ferritin Translation and Iron Hemostasis.
Hognon, Biomolecules 2021 - GeneRIF: The Iron Maiden. Cytosolic Aconitase/IRP1 Conformational Transition in the Regulation of Ferritin Translation and Iron Hemostasis.
- Cellular iron sensing and regulation: Nuclear IRP1 extends a classic paradigm.
Hernández-Gallardo, Biochimica et biophysica acta. Molecular cell research 2020 (PubMed)- GeneRIF: Cellular iron sensing and regulation: Nuclear IRP1 extends a classic paradigm.
- Predicted loss and gain of function mutations in ACO1 are associated with erythropoiesis.
Oskarsson, Communications biology 2020 - GeneRIF: Predicted loss and gain of function mutations in ACO1 are associated with erythropoiesis.
- Pathological changes in Sertoli cells and dysregulation of divalent metal transporter 1 with iron responsive element in the testes of idiopathic azoospermia patients.
Jing, Andrologia 2018 (PubMed)- GeneRIF: Results indicate that there is a dysregulation of DMT1 + IRE in IA testes, which might due to the up-regulation of IRP1 and HIF-1A.
- Evaluation of the iron regulatory protein-1 interactome.
Connell, Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 2018 (PubMed)- GeneRIF: Evaluation of the iron regulatory protein-1 interactome has been presented.
- More
- Involvement of the iron regulatory protein from Eisenia andrei earthworms in the regulation of cellular iron homeostasis
Procházková, PloS one 2014 - “...(%) Hs Mm Rn Gg Ss Dr At Pf Dm Aa Ms Pl Ce Ea NP_002188 Homo sapiens IRP-1 100 93 93 88 83 82 59 53 67 68 68 69 64 67 CAA43455 Mus musculus IREBP 100 96 88 83 83 59 53 68 69...”
- Leishmania infection upregulates and engages host macrophage Argonaute 1, and system-wide proteomics reveals Argonaute 1-dependent host response
Moradimotlagh, Frontiers in immunology 2023 - “...gamma GMFG O60234 0.209 -0.381 N/A N/A Salmonella ( 70 ) Cytoplasmic aconitate hydratase ACO1 P21399 0.358 -0.621 N/A Aco1 is one of the genes related to the response to Leishmania ssp ( 71 ). Mycobacterium tuberculosis ( 72 ) Aconitate hydratase, mitochondrial ACO2 Q99798 0.158...”
- Human microRNA (miR-20b-5p) modulates Alzheimer's disease pathways and neuronal function, and a specific polymorphism close to the MIR20B gene influences Alzheimer's biomarkers.
Wang, Molecular psychiatry 2022 - “...beta Tau IDE P14735 Insulin-degrading enzyme Clearance + IL1A P01583 Interleukin-1 alpha Amyloid + IRP1 P21399 Iron-responsive element-binding protein 1 Amyloid IRP2 P48200 Iron-responsive element-binding protein 2 Amyloid MAPK13 O15264 Mitogen-activated protein kinase 13 Tau MAPT P10636 Microtubule-associated protein tau Tau + MME P08473 Membrane metallo-endopeptidase...”
- The Expression of Rab8, Ezrin, Radixin and Moesin in the Ciliary Body of Cynomolgus Monkeys.
Tanabe, Juntendo Iji zasshi = Juntendo medical journal 2022 - “...P04080 11132.6 12.20 1 50 Cytochrome c P99999 11741.1 33.30 13 51 Cytoplasmic aconitate hydratase P21399 98336.7 1.60 1 52 Cytoskeleton-associated protein 5 Q14008 225364.6 0.80 1 53 Dachshund homolog 1 Q9UI36 78513.4 2.00 1 54 D-dopachrome decarboxylase P30046 12703.7 9.30 2 55 Decorin P07585 39721.9...”
- ITRAQ-based quantitative proteomic analysis reveals that VPS35 promotes the expression of MCM2-7 genes in HeLa cells.
Hong, Scientific reports 2022 - “...1.92E44 2.47 6.01E79 A0A024RAI5 BIN1 1.44 2.54E05 1.37 3.64E04 P31949 S100A11 1.74 1.19E11 1.75 9.70E14 P21399 ACO1 1.25 5.34E09 1.45 7.12E23 Q15651 HMGN3 P 1.33 2.21E03 1.68 3.22E04 P05204 HMGN2 1.51 2.08E12 1.77 1.02E25 A0A024R9Q1 THBS1 1.29 7.52E04 1.54 5.38E03 A0A172Q3A8 FOLR1 1.21 1.72E05 2.59 7.53E14...”
- Multi-Omics Reveals Mechanisms of Partial Modulation of COVID-19 Dysregulation by Glucocorticoid Treatment.
Spick, International journal of molecular sciences 2022 - “...3 0.46 Decreased Coagulation factor IX P00740 4.22 10 3 1.30 Increased Cytoplasmic aconitate hydratase P21399 5.35 10 3 0.78 Decreased Transgelin Q01995 5.50 10 3 0.75 Decreased Carboxypeptidase B2 Q96IY4 5.82 10 3 1.35 Increased ijms-23-12079-t004_Table 4 Table 4 Metabolomics: pathways identified as differentially expressed...”
- Lack of evidence of significant homology of SARS-CoV-2 spike sequences to myocarditis-associated antigens.
Marrama, EBioMedicine 2022 - “...TPM2 P07951 Review Literature Tropomyosin alpha-3 chain TPM3 P06753 Review Literature Cytoplasmic aconitate hydratase ACO1 P21399 Review Literature ADP/ATP translocase 1 SLC25A4 P12235 Review Literature Creatine kinase B-type CKB P12277 Review Literature Creatine kinase S-type, mitochondrial CKMT2 P17540 Review Literature Creatine kinase U-type, mitochondrial CKMT1A P12532...”
- Dynamic Changes of Urine Proteome in Rat Models Inoculated with Two Different Hepatoma Cell Lines.
Zhang, Journal of oncology 2021 - “...gamma-lyase 2.64 2.04 Melanoma P14174 Macrophage migration inhibitory factor 2.62 Serum Gastrointestinal tract (GIT) malignancy P21399 Cytoplasmic aconitate hydratase 2.57 Q86YJ6 Threonine synthase-like 2 2.50 P02511 Alpha-crystallin B chain 2.49 Tissue Colorectal cancer O94760 Dimethylargininase-1 2.48 Tissue Prostate cancer Q96S37 Solute carrier family 22 member 12...”
- Potential Role of Epithelial Endoplasmic Reticulum Stress and Anterior Gradient Protein 2 Homologue in Crohn's Disease Fibrosis.
Vieujean, Journal of Crohn's & colitis 2021 - More
NP_001147431 aconitase 2 isoform 1 from Zea mays
B6SRL2 Aconitate hydratase from Zea mays
46% identity, 99% coverage
- The aconitate hydratase family from Citrus
Terol, BMC plant biology 2010 - “...Aco4 40 Z. mays NM_001165757 NP_001159229 LOC100304315 Aco1 188 NM_001143012 NP_001136484 LOC100216599 Aco4 301 NM_001153959 NP_001147431 - Aco5 128 A multiple alignment with 48 Aco sequences was carried out, and a phylogenetic analysis was performed as described in Methods. The tree obtained (Figure 2 ) shows...”
- Proteomics analysis reveals a dynamic diurnal pattern of photosynthesis-related pathways in maize leaves
Feng, PloS one 2017 - “...12.75 2 400 45.40 6.39 B4G249 uncharacterized LOC100274592 (gpm541) 7 14.08 3 412 46.19 6.55 B6SRL2 aconitase 2 (aco2) 7 6.85 4 905 98.88 6.48 B6TD54 uncharacterized LOC100282948 7 4.50 2 422 46.61 5.72 B6TQ36 pyruvate dehydrogenase E1 component subunit beta 7 5.05 2 396 42.29...”
ETH_00020300 uncharacterized protein from Eimeria tenella
48% identity, 97% coverage
- Phosphoproteomic Comparison of Four Eimeria tenella Life Cycle Stages
Ma, International journal of molecular sciences 2021 - “...including tricarboxylic acid (TCA) metabolism ( p value = 0.006) (aconitate hydratase ( ETH_00025665 and ETH_00020300 )), ATP hydrolysis-coupled ion transmembrane transport ( p value = 0.014) (calcium-transporting ATPase ( ETH_00002655 ) and P-type Ca 2+ -ATPase ( ETH_00006965 )), positive regulation of exocytosis ( p...”
- “...= 0.005), and TCA cycle ( p value = 0.018) subcategories, including aconitate hydratase ( ETH_00020300 ) ( Figure 3 A). There was a clear phosphorylation peak at the USO phase, which then decreased steadily for proteins in cluster 2. Proteins in this profile were linked...”
- The 2-methylcitrate cycle is implicated in the detoxification of propionate in Toxoplasma gondii
Limenitakis, Molecular microbiology 2013 - “...PrpE NCLIV_032540 ETH_00007195 PrpC NCLIV_024780 ETH_00026655 PrpD NCLIV_013410 ETH_00015105 PrpB NCLIV_000780 Contig 1606+713 Acn NCLIV_046260 ETH_00020300 PF13_0229 TP01_1050 NCLIV_033680 ETH_00021115 PF08_0031 cgd1_600 TP02_0450 Abbreviations Bb Pm Pt Tt PrpE XP_001460124 XP_001025576 PrpC XP_002787782 XP_001447471 XP_001023516 PrpD EER04808 XP_001457033 EAS06482 PrpB XP_002772756 XP_001459690 EAR89396 Acn BBOV_III011790 XP_002765934...”
BAD_RS05595 aconitate hydratase AcnA from Bifidobacterium adolescentis ATCC 15703
48% identity, 97% coverage
- Combining of transcriptome and metabolome analyses for understanding the utilization and metabolic pathways of Xylo-oligosaccharide in Bifidobacterium adolescentis ATCC 15703
Yang, Food science & nutrition 2019 - “...BAD_RS08480 1.06 bglB Betaglucosidase 137.7 66.25 ko01100/ko01110/ko00500/ko00460 BAD_RS07395 1.05 bgaB Betagalactosidase I 175.7 84.64 ko00052 BAD_RS05595 1.05 acn Aconitate hydratase 137.1 283.08 ko01100/ko01110/ko01130/ko01230/ko01120/ko01200/ko01210/ko00630/ko00020 BAD_RS07575 1.25 glgE Alpha1,4glucanmaltose1phosphate maltosyltransferase 461.18 1,093.58 ko01100/ko00500 John Wiley & Sons, Ltd 3.4 Validation of transcript abundance using qRTPCR To verify the...”
- “...to pyruvate metabolism and the TCA cycle (ko00620; ko00020) was only aconitate hydratase (encoded by BAD_RS05595), metabolites isocitrate (meta_211) was also upregulated in B.adolescentis 15703 grown on XOS. Meanwhile, phosphoenolpyruvateprotein phosphotransferase (BAD_RS00875), Citrate (meta_293) and Pyruvate (meta_8) were significantly downregulated in B.adolescentis 15703 grown on XOS....”
I3LJW4 Cytoplasmic aconitate hydratase from Sus scrofa
49% identity, 90% coverage
TA17020 iron-responsive element binding protein/aconitase, putative from Theileria annulata
51% identity, 91% coverage
- Proteomic analysis of the Theileria annulata schizont
Witschi, International journal for parasitology 2013 - “...(TA10425); PK, pyruvate kinase (TA11540, TA10915); LDH, lactate dehydrogenase (TA09590); PC, pyruvate carboxylase; ACO, aconitase (TA17020); IDH, isocitrate dehyrogenase (TA10850); OGDH, oxoglutarate dehydrogenase (TA05275, TA08530); SCS, succinyl coenzyme A synthetase (TA02815, TA10625); SDH, succinate dehydrogenase (TA19430, TA03455); FH, fumarate hydratase; MDH, malate dehydrogenase (TA18100); CS, citrate...”
ACOHC_MOUSE / P28271 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Mus musculus (Mouse) (see paper)
P28271 aconitate hydratase (EC 4.2.1.3) from Mus musculus (see paper)
NP_031412 cytoplasmic aconitate hydratase from Mus musculus
48% identity, 95% coverage
- function: Bifunctional iron sensor that switches between 2 activities depending on iron availability (By similarity). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed:1956798).
function: Conversely, when cellular iron levels are high, binds a 4Fe- 4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis- aconitate.
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Interacts (when associated with the 4Fe-4S) with FBXL5. Interacts with frataxin(81-210). - A Murine Model of Maternal Micronutrient Deficiencies and Gut Inflammatory Host-microbe Interactions in the Offspring.
Holani, Cellular and molecular gastroenterology and hepatology 2024 - “...9.14052870878679E-06 0.013235485570323300 Up-regulated P24270 Cat 1.693930533094730 3.22382719790435E-09 4.7422498081173E-06 Down-regulated P24549 Aldh1a1 1.0447106634966300 1.41518172923822E-05 0.020406920535615200 Down-regulated P28271 Aco1 0.7917686970933590 3.45645356990867E-05 0.04946185058539300 Down-regulated P28843 Dpp4 0.9711436627995890 1.62290410551764E-05 0.023321131996288500 Down-regulated P29758 Oat 0.9232173792610570 4.47535539220895E-06 0.0064892653187029700 Down-regulated P34884 Mif 0.8806908186675840 3.43356753663742E-05 0.0491686871246479 Up-regulated P34914 Ephx2 2.93616928671433 2.83950119037278E-07 4.15702974270575E-04 Down-regulated P48758...”
- Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis
Banfi, Antioxidants (Basel, Switzerland) 2023 - “...9 0.006 2.32 NEG Glycerol-3-phosphate dehydrogenase [NAD(+)]_ cytoplasmic OS = Mus musculus GN = Gpd1 P28271 3 0.004 2.18 NEG Cytoplasmic aconitate hydratase OS = Mus musculus GN = Aco1 P16125 2 0.011 2.03 NEG L-lactate dehydrogenase B chain OS = Mus musculus GN = Ldhb...”
- Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice.
Ohira, NPJ microgravity 2021 - “...(Q9DCW4) FC=1.15, P =1.5E2 * FC=1.19, P =3.5E3 FC=1.01, P =6.4E1 Tricarboxylic acid cycle Aco1 (P28271) * FC=1.21, P =5.9E3 FC=1.21, P =8.0E2 FC=0.96, P =3.7E1 Idh2 (P54071) * FC=1.43, P =8.5E4 * FC=1.45, P =3.2E4 FC=0.98, P =6.3E1 Mdh1 (P14152) FC=1.05, P =1.4E1 * FC=1.09,...”
- The Proteomic Signature of Intestinal Acute Rejection in the Mouse.
Oltean, Metabolites 2021 - “...Trans-2,3-enoyl-CoA reductase 0.78 Oxidoreductase Lipid metabolism Q9JII6 Ak1a1 Alcohol dehydrogenase 0.77 Dehydrogenase/reductase Small molecule biochemistry P28271 Aco1 Cytoplasmic aconitate hydratase 0.77 Lyase, RNA-binding Cellular growth and proliferation Q9JLQ0 Cd2ap CD2-associated protein 0.76 Adapter protein Cell cycle Q99KI0 Acon Aconitate hydratase, mitochondrial 0.76 Lyase Cellular growth and...”
- Dataset on the mass spectrometry-based proteomic profiling of the kidney from wild type and the dystrophic mdx-4cv mouse model of X-linked muscular dystrophy.
Dowling, Data in brief 2020 - “...3 12.7193 0.00370 1.29 P11881 Inositol 1,4,5-trisphosphate receptor type 1 Itpr1 5 17.8101 3.12E-05 1.29 P28271 Cytoplasmic aconitate hydratase Aco1 2 4.3600 0.00364 1.28 Q8VCT4 Carboxylesterase 1D Ces1d 2 6.6092 0.01551 1.28 Q91YI0 Argininosuccinate lyase Asl 2 8.7531 0.00032 1.27 Q64471 Glutathione S-transferase theta-1 Gstt1 2...”
- 2D DIGE proteomic analysis reveals fasting-induced protein remodeling through organ-specific transcription factor(s) in mice
Kamata, FEBS open bio 2018 - “...Catalase P70423 Cat Mm.4215 115 22% 12/21 60043 7.72 66 1.21 0.039 Cytoplasmic aconitate hydratase P28271 Aco1 Mm.331547 223 52% 37/77 98691 7.23 67 1.20 0.009 Peroxiredoxin1 P35700 Prdx1 Mm.30929 90 47% 9/27 22390 8.26 68 1.18 0.038 AcylCoA synthetase family member 2, mitochondrial Q8VCW8 Acsf2...”
- Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles
Durcin, Journal of extracellular vesicles 2017 - “...liver type 2.2 1.7E-02 0.5/2.5 NA P19096 FAS Fatty acid synthase 3.3 3.2E-03 28/20 6.6E-01 P28271 ACOC Cytoplasmic aconitate hydratase 2.7 3.5E-04 3.0/3.0 NA P54310 LIPS Hormone-sensitive lipase 2.5 3.5E-04 3.0/4 NA P97873 LOXL1 Lysyl oxidase homologue 1 5.2 3.3E-02 6.5/3.5 NA Q00612 G6PD1 Glucose-6-phosphate 1-dehydrogenase...”
- Integrated analysis of proteome and transcriptome changes in the mucopolysaccharidosis type VII mouse hippocampus
Parente, Molecular genetics and metabolism 2016 - “...2-like P28740 Kif2a 0.011 -3.52 kinesin family member 2A P21619 Lmnb2 0.042 -3.60 lamin B2 P28271 Aco1 0.020 -3.61 aconitase 1 P60469 Ppfia3 0.021 -3.65 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 Q8BRT1 Clasp2 0.016 -3.67 CLIP associating protein 2...”
- “...Q9CZD3 Gars 0.009 -3.06 Glycyl-tRNA synthetase Q9CQX8 Mrps36 0.028 -3.13 28S ribosomal protein S36, mitochondrial P28271 Aco1 0.020 -3.61 Cytoplasmic aconitate hydratase Q9D8S9 Bola1 BolA-like protein 1 Q9R1Z7 Pts 6-pyruvoyl tetrahydrobiopterin synthase P58059 Mrps21 28S ribosomal protein S21, mitochondrial Q9D1L0 Chchd2 Coiled-coil-helix-domain-containing protein 2, mitochondrial Q9CQV7...”
- More
- Iron regulatory proteins 1 and 2 have opposing roles in regulating inflammation in bacterial orchitis.
Ghatpande, JCI insight 2024 - GeneRIF: Iron regulatory proteins 1 and 2 have opposing roles in regulating inflammation in bacterial orchitis.
- Diurnal control of iron responsive element containing mRNAs through iron regulatory proteins IRP1 and IRP2 is mediated by feeding rhythms.
Nadimpalli, Genome biology 2024 - GeneRIF: Diurnal control of iron responsive element containing mRNAs through iron regulatory proteins IRP1 and IRP2 is mediated by feeding rhythms.
- Iron regulatory protein 1 is required for the propagation of inflammation in inflammatory bowel disease.
Fahoum, The Journal of biological chemistry 2024 - GeneRIF: Iron regulatory protein 1 is required for the propagation of inflammation in inflammatory bowel disease.
- H2S regulation of iron homeostasis by IRP1 improves vascular smooth muscle cell functions.
Zhu, Cellular signalling 2023 (PubMed)- GeneRIF: H2S regulation of iron homeostasis by IRP1 improves vascular smooth muscle cell functions.
- Interleukin-6 regulates iron-related proteins through c-Jun N-terminal kinase activation in BV2 microglial cell lines.
Zhou, PloS one 2017 - GeneRIF: IL-6 likely up-regulates IRP1 and DMT1 expression and down-regulates FPN1 expression in BV2 microglial cells through JNK signaling pathways
- Iron-induced epigenetic abnormalities of mouse bone marrow through aberrant activation of aconitase and isocitrate dehydrogenase.
Yamamoto, International journal of hematology 2016 (PubMed)- GeneRIF: Using whole RNA sequencing of bone marrow cells in iron-overloaded mice, it was observed that Idh1 and Aco1, enzymes involved in the TCA cycle, were elevated.
- Cytosolic aconitase activity sustains adipogenic capacity of adipose tissue connecting iron metabolism and adipogenesis.
Moreno, FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 (PubMed)- GeneRIF: Aco1 KD in fully differentiated 3T3-L1 adipocytes decreased lipogenic, Idh1, Adipoq, and Glut4 gene expression.
- Iron regulatory protein 1 sustains mitochondrial iron loading and function in frataxin deficiency.
Martelli, Cell metabolism 2015 (PubMed)- GeneRIF: results uncover an unexpected protective role of IRP1 in pathological conditions associated with altered Fe-S metabolism
- More
NP_059017 cytoplasmic aconitate hydratase from Rattus norvegicus
48% identity, 95% coverage
- Inhibitory effect of homocysteine on rat neural stem cell growth in vitro is associated with reduced protein levels and enzymatic activities of aconitase and respiratory complex III.
Zhang, Journal of bioenergetics and biomembranes 2017 (PubMed)- GeneRIF: Proteomic analysis revealed that the expression levels of two mitochondrial proteins, cytochrome bc1 complex2 (UQCRC2, the major component of electron transport chain complex III) and aconitase (an enzyme involved in the tricarboxylic acid cycle), were decreased by homocysteine in neural stem cells
- Iron-dependent turnover of IRP-1/c-aconitase in kidney cells.
Liu, Metallomics : integrated biometal science 2015 (PubMed)- GeneRIF: Iron increases turnover of IRP-1 in kidney cells, while increasing aconitase activity, suggesting that the apoprotein (aconitase-inactive) form is not exclusively responsible for turnover.
- Metabolic control analysis of mitochondrial aconitase: influence over respiration and mitochondrial superoxide and hydrogen peroxide production.
Scandroglio, Free radical research 2014 (PubMed)- GeneRIF: These results indicate that aconitase can exert control over respiration (with tissue specificity) and support the hypothesis that inactivation of aconitase may provide a control mechanism to prevent O2(-) and H2O2 formation by the respiratory chain.
- Cadmium-induced aggregation of iron regulatory protein-1.
Liu, Toxicology 2014 (PubMed)- GeneRIF: Polymer-dependent translocation of IRP-1 in Cd(2+)-exposed cells may underlie effects of Cd(2+) on iron homeostasis.
- Pro-inflammatory cytokines modulate iron regulatory protein 1 expression and iron transportation through reactive oxygen/nitrogen species production in ventral mesencephalic neurons.
Wang, Biochimica et biophysica acta 2013 (PubMed)- GeneRIF: Results suggest that IL-1beta and TNF-alpha released by microglia might contribute to iron accumulation in ventral mesencephalic neurons by upregulating IRP1 and hepcidin levels through reactive oxygen/nitrogen species production.
- Cell density-dependent shift in activity of iron regulatory protein 1 (IRP-1)/cytosolic (c-)aconitase.
Popovic, Metallomics : integrated biometal science 2012 (PubMed)- GeneRIF: The RNA-binding and aconitase forms of IRP-1 can undergo interconversion dependent on the density of cells growing in culture.
- Regulation of iron-related molecules in the rat hippocampus: sex- and age-associated differences.
Thulluri, Annals of clinical and laboratory science 2012 (PubMed)- GeneRIF: The expression of HO1, FTH, and IRP1 mRNAs is higher in the female hippocampus than that observed in male rats.
- Angiotensin receptor blockade recovers hepatic UCP2 expression and aconitase and SDH activities and ameliorates hepatic oxidative damage in insulin resistant rats.
Montez, Endocrinology 2012 - GeneRIF: Angiotensin receptor blockade recovers hepatic UCP2 expression and aconitase and SDH activities and ameliorates hepatic oxidative damage in insulin resistant rats
- More
XP_008251386 cytoplasmic aconitate hydratase isoform X1 from Oryctolagus cuniculus
47% identity, 97% coverage
BBOV_III011790 aconitate hydratase 1 family protein from Babesia bovis T2Bo
48% identity, 98% coverage
R0J775 Cytoplasmic aconitate hydratase (Fragment) from Anas platyrhynchos
48% identity, 95% coverage
BBM_III04870 aconitate hydratase 1 from Babesia microti strain RI
47% identity, 97% coverage
ACOHC_RABIT / Q01059 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Oryctolagus cuniculus (Rabbit) (see paper)
47% identity, 97% coverage
- function: Bifunctional iron sensor that switches between 2 activities depending on iron availability (By similarity). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization (PubMed:17185597). Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed:17185597).
function: Conversely, when cellular iron levels are high, binds a 4Fe- 4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis- aconitate.
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Interacts (when associated with the 4Fe-4S) with FBXL5. Interacts with frataxin(81-210). - Iron metabolic pathways in the processes of sponge plasticity.
Finoshin, PloS one 2020 - “...numbering corresponds to the rabbit IRP1. Triangles, residues contacting with IRE, O.cun, O.cuniculus (UniProt ID: Q01059); H.duj, H . dujardini ; H.pan, H . panicea ; A.que, A . queenslandica (UniProt ID: A0A1X7VVE4). (PDF) Click here for additional data file. S4 Fig HIFa/SIM and ARNT domains...”
NP_477371 iron regulatory protein 1A from Drosophila melanogaster
47% identity, 98% coverage
Q9NFX3 aconitate hydratase (EC 4.2.1.3) from Drosophila melanogaster (see paper)
47% identity, 98% coverage
NP_524303 iron regulatory protein 1B from Drosophila melanogaster
47% identity, 98% coverage
Q9NFX2 aconitate hydratase (EC 4.2.1.3) from Drosophila melanogaster (see paper)
47% identity, 98% coverage
ACOHC_BOVIN / Q0VCU1 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Bos taurus (Bovine) (see paper)
47% identity, 95% coverage
- function: Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron- responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.
function: Conversely, when cellular iron levels are high, binds a 4Fe- 4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis- aconitate.
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Interacts (when associated with the 4Fe-4S) with FBXL5. Interacts with frataxin(81-210). - Identification of 56 Proteins Involved in Embryo-Maternal Interactions in the Bovine Oviduct
Banliat, International journal of molecular sciences 2020 - “...(kDa) OF-Treated: Control Ratio ACLY ATP-citrate synthase Q32PF2 120 T 1 ACO1 Cytoplasmic aconitate hydratase Q0VCU1 98 T 1 PNP Purine nucleoside phosphorylase P55859 32 T 1 BLVRA Biliverdin reductase A A5D7K0 34 9.9 A2M Alpha-2-macroglobulin Q7SIH1 168 7 HBB Hemoglobin subunit beta P02070 16 6.7...”
- Transcriptome analysis of genes and pathways associated with metabolism in Scylla paramamosain under different light intensities during indoor overwintering.
Li, BMC genomics 2020 - “...P13086 0.5567 0.0452 0.4452 0.0016 Malate dehydrogenase Q3T145 0.3996 0.0010 0.4326 0.0006 Cytoplasmic aconitate hydratase Q0VCU1 0.4902 0.0109 0.5738 0.0262 Isocitrate dehydrogenase [NADP] Q04467 0.4560 0.0079 0.5210 0.0216 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex Q9D2G2 0.4379 0.0020 Succinate dehydrogenase cytochrome b560 subunit P70097 0.4841 0.0074...”
- Discovery and Quantification of Nonhuman Proteins in Human Milk
Zhu, Journal of proteome research 2019 - “...[R].GYKHTLNQIDSVK.[V] 2 Alpha-2-HS-glycoprotein Bos taurus and Ovis aries 68 6 [K].SPPFFEDLTLDLQPPK.[S] 4 Cytoplasmic aconitate hydratase Q0VCU1 Bos taurus 47 6 [R].ADSLKKNQDLEFER.[N] 4 Cytoplasmic aconitate hydratase Q0VCU1 Bos taurus 61 6 [K].TVDNFVALATGEKGFGYKDSK.[F] 3 Peptidyl-prolyl cistrans isomeraseB P80311 Bos taurus 44 5 [K].GFGYKDSK.[F] 1 Peptidyl-prolyl cistrans isomeraseB P80311...”
W5PZ94 Cytoplasmic aconitate hydratase from Ovis aries
47% identity, 95% coverage
- Proteomics and metabolomics combined study on endopathic changes of water-soluble precursors in Tan lamb during postmortem aging
Ji, Food science & nutrition 2022 - “...1.16E04 W5PWE9 Serum albumin 3.87 5.22E07 W5PX04 Myosin binding protein C, fast type 4.34 1.21E05 W5PZ94 Aconitase 1 0.48 3.36E02 W5Q160 Keratin 10 0.25 2.45E04 W5Q611 Keratin 1 0.18 9.00E05 W5Q6U0 Fatty acid synthase 0.41 1.92E02 W5Q8M3 Amyloalpha1, 6glucosidase, 4alphaglucanotransferase 2.13 3.04E04 W5Q9G8 EnoylCoA delta isomerase...”
- “...0.66 W5PQL7 Tropomyosin 2 3.69 0.22 W5PX04 Myosin binding protein C, fast type 4.34 1.36 W5PZ94 Aconitase 1 0.48 W5Q6U0 Fatty acid synthase 0.41 W5Q8M3 Amyloalpha1, 6glucosidase, 4alphaglucanotransferase 2.13 W5Q9G8 EnoylCoA delta isomerase 1 0.49 W5QD16 Myosin light chain 1 10.5 0.52 A9YUY8 Adipocyte fatty acidbinding...”
Tneu_0614 aconitate hydratase from Thermoproteus neutrophilus V24Sta
48% identity, 97% coverage
Q90875 Cytoplasmic aconitate hydratase from Gallus gallus
47% identity, 95% coverage
ACOHC_RAT / Q63270 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Rattus norvegicus (Rat) (see paper)
Q63270 aconitate hydratase (EC 4.2.1.3) from Rattus norvegicus (see paper)
47% identity, 95% coverage
- function: Bifunctional iron sensor that switches between 2 activities depending on iron availability (By similarity). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization (PubMed:16144863). Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed:16144863).
function: Conversely, when cellular iron levels are high, binds a 4Fe- 4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis- aconitate.
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Interacts (when associated with the 4Fe-4S) with FBXL5. Interacts with frataxin(81-210). - PPAR beta/gamma mediates the antihypertensive activity of a synbiotic preparation of Bifidobacterium lactis and Lactobacillus acidophilus in spontaneous hypertensive rats
Huang, Heliyon 2024 - “...0.5546 0.0022 0.6982 0.8360 4 P48037 Annexin A6 ANXA6 48 0.5200 0.0013 1.6293 0.0081 5 Q63270 Cytoplasmic aconitate hydratase ACOC 32 0.6668 0.0478 1.3552 0.3422 6 P22985 Xanthine dehydrogenase/oxidase XDH 31 0.7447 0.0479 1.1376 0.2121 7 Q62635 Mucin-2 (Fragment) MUC2 29 0.3802 0.0007 1.0000 0.8674 8...”
- Brown Adipose Tissue Sheds Extracellular Vesicles That Carry Potential Biomarkers of Metabolic and Thermogenesis Activity Which Are Affected by High Fat Diet Intervention.
Camino, International journal of molecular sciences 2022 - “...MDHM_RAT P04636 Malate dehydrogenase, mitochondrial 0.047 2.22 PLPP3_RAT P97544 Phospholipid phosphatase 3 0.030 2.20 ACOC_RAT Q63270 Cytoplasmic aconitate hydratase 0.004 2.19 SBP1_RAT Q8VIF7 Methanethiol oxidase 0.041 2.19 PLBL1_RAT Q5U2V4 Phospholipase B-like 1 0.005 2.16 LDHB_RAT P42123 L-lactate dehydrogenase B chain 0.000 2.15 AP1B1_RAT P52303 AP-1 complex...”
- Functional proteomic analysis of corticosteroid pharmacodynamics in rat liver: Relationship to hepatic stress, signaling, energy regulation, and drug metabolism.
Ayyar, Journal of proteomics 2017 - “...Energy metabolism - transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) UP Q63270 Aco1 Cytoplasmic aconitate hydratase Iron sensor UP Q68FS4 Lap3 Cytosol aminopeptidase Processing and regulation of turnover of intracellular proteins UP Q6Q0N1 Cndp2 Cytosolic non-specific dipeptidase Hydrolyzes a variety of dipeptides...”
- Proteomic responses of skeletal and cardiac muscle to exercise.
Burniston, Expert review of proteomics 2011 - “...[ 43 ] Lactate dehydrogenase A P04642 [ 35 ] Tricarboxylic acid cycle Acontiase 2 Q63270 [ 35 ] Maltate dehydrogenase, mitochondrial P04636 [ 43 ] Succinate CoA B2GV06 [ 35 ] Succinate dehydrogenase [ubiquinone] flavoprotein subunit P31040 [ 44 ] Oxoglutarate (alpha-ketoglutarate) dehydrogenase Q5XI78 [...”
- “...aminotransferase P00507 [ 36 , 63 ] Citrate synthase Q8VHF5 [ 63 ] Aconitase * Q63270 [ 36 ] Isocitrate dehydrogenase [NAD] subunit alpha Q99NA5 [ 56 ] Dihydrolipoamide S-succinyltransferase Q01205 [ 50 ] Antioxidant and heat shock proteins Heat shock protein 20 P97541 [ 36...”
- Protective effects and potential mechanisms of Pien Tze Huang on cerebral chronic ischemia and hypertensive stroke
Zhang, Chinese medicine 2010 - “...NP_114039 PHB -2.13 561 Prohibitin P10860 NP_036702 DHE3 -2.03 443 Glutamate dehydrogenase 1, mitochondrial precursor Q63270 NP_059017 ACOC +2.00 154 Cytoplasmic aconitate hydratase * - denotes down-regulated and + denotes up-regulated comparing the ischemia with PZH group (1B) to the ischemia control group (2B). Table 7...”
- Neuroproteomics: a biochemical means to discriminate the extent and modality of brain injury.
Ottens, Journal of neurotrauma 2010 - High-throughput identification of IMCD proteins using LC-MS/MS.
Pisitkun, Physiological genomics 2006
ETH_00025665 aconitate hydratase, putative from Eimeria tenella
46% identity, 98% coverage
- Phosphoproteomic Comparison of Four Eimeria tenella Life Cycle Stages
Ma, International journal of molecular sciences 2021 - “...biological processes including tricarboxylic acid (TCA) metabolism ( p value = 0.006) (aconitate hydratase ( ETH_00025665 and ETH_00020300 )), ATP hydrolysis-coupled ion transmembrane transport ( p value = 0.014) (calcium-transporting ATPase ( ETH_00002655 ) and P-type Ca 2+ -ATPase ( ETH_00006965 )), positive regulation of exocytosis...”
S101446_02412 aconitate hydratase AcnA from Komagataeibacter europaeus
46% identity, 98% coverage
PVX_083005 aconitate hydratase I, putative from Plasmodium vivax
45% identity, 98% coverage
- A systems-based analysis of Plasmodium vivax lifecycle transcription from human to mosquito
Westenberger, PLoS neglected tropical diseases 2010 - “...subunit of succinate dehydrogenase (PVX_111005, PF10_0334); malate:quinone oxidoreductase (PVX_113980, PFF0815w); IRP-like protein (iron regulatory protein-like) (PVX_083005, PF13_0229); fumarate hydratase (PVX_099805, PFI1340w); ATP-specific succinyl-CoA synthetase beta subunit (PVX_084960, PF14_0295); 2-oxoglutarate dehydrogenase E1 component (PVX_089325, PF08_0045); dihydrolipoamide acyltransferase (PVX_119310, PFC0170c); succinyl-CoA synthetase alpha subunit (PVX_091100, PF11_0097); iron-sulfur subunit...”
APA386B_1323 aconitate hydratase AcnA from Acetobacter pasteurianus 386B
C7JG48 Aconitate hydratase from Acetobacter pasteurianus (strain NBRC 105184 / IFO 3283-01)
46% identity, 98% coverage
PF3D7_1342100, XP_001350142 aconitate hydratase from Plasmodium falciparum 3D7
44% identity, 97% coverage
- PfMFR3: A Multidrug-Resistant Modulator in Plasmodium falciparum
Rocamora, ACS infectious diseases 2021 - “...acid cycle. This reaction is catalyzed by aconitate hydratase, or aconitase, an enzyme encoded by PF3D7_1342100 or pfirp that has also been found to be located in the mitochondrion. 54 While not essential for asexual blood-stage growth, PfIRP is important to sexual-stage development, since knocking out...”
- Divergent acyl carrier protein decouples mitochondrial Fe-S cluster biogenesis from fatty acid synthesis in malaria parasites
Falekun, eLife 2021 - “...cluster proteins function within the P. falciparum mitochondrion ( Haussig et al., 2014 ). Aconitase (PF3D7_1342100) and succinate dehydrogenase (PF3D7_1212800) are dispensable for blood-stage parasite growth ( Ke et al., 2015 ). Ferredoxin (PF3D7_1214600) is expected to be essential ( Gomes et al., 2015 ), but...”
- Novel Molecular Synapomorphies Demarcate Different Main Groups/Subgroups of Plasmodium and Piroplasmida Species Clarifying Their Evolutionary Relationships
Sharma, Genes 2019 - “...Subgenus Laverania a Conserved hypothetical protein XP_001349841 S43 1 aa ins 322365 Aconitate hydratase protein XP_001350142 S44 1 aa ins 450494 Conserved Plasmodium protein XP_024329193 S45 1 aa ins 528575 Cation-transporting ATPase XP_001349175.1 S46 1 aa ins 576633 Serine/threonine protein kinase XP_001349887 S47 2 aa ins...”
- The antimalarial drug primaquine targets Fe-S cluster proteins and yeast respiratory growth
Lalève, Redox biology 2016 - “...[48] , compared to the asexual blood stage. Remarkably, using P. falciparum knockout lines, aconitase (PF3D7_1342100) was found to be essential for gametocytogenesis [46] , [47] . The parasite aconitase is likely to be ROS-sensitive as are the yeast and mammalian enzymes. Interestingly, the parasite fumarase...”
- Genetic investigation of tricarboxylic acid metabolism during the Plasmodium falciparum life cycle
Ke, Cell reports 2015 - “...flavoprotein subunit ( SDH , PF3D7_1034400), CS ( CS , PF3D7_1022500), aconitase ( Aco , PF3D7_1342100) and isocitrate dehydrogenase ( IDH , PF3D7_1345700) ( Figures S1, S2 ). In addition, we also produced three double KO lines: 1) KDH / SCS , which should prevent glutamine-derived...”
- Identification of vital and dispensable sulfur utilization factors in the Plasmodium apicoplast
Haussig, PloS one 2014 - “...0.5453 PBANKA_130330 PF3D7_1439400 ubiquinol-cytochrome c reductase iron-sulfur subunit, putative /++ no SP possibly 0.9782 PBANKA_135520 PF3D7_1342100 aconitate hydratase /++ no SP possibly 0.8460 PBANKA_142880 PF3D7_1212800 iron-sulfur subunit of succinate dehydrogenase /++ no SP possibly 0.1521 PBANKA_143040 PF3D7_1214600 adrenodoxin-type ferredoxin, putative /++ no SP possibly 0.8299 NUCLEUS...”
- An iron regulatory-like protein expressed in Plasmodium falciparum displays aconitase activity.
Hodges, Molecular and biochemical parasitology 2005 (PubMed)- GeneRIF: Further understanding of the role of PfIRPa/aconitase in the regulation of P. falciparum homeostasis may contribute towards the development of novel antimalarial strategies against plasmodial species.
PBANKA_135520 aconitate hydratase, putative from Plasmodium berghei ANKA
43% identity, 99% coverage
- Plasmodium female gamete surface HSP90 is a key determinant for fertilization
Cha, mBio 2024 - “...). Three parasite proteins, PBANKA_143730 (endoplasmin homolog precursor), PBANKA_080570 (heat shock protein 90, HSP90), and PBANKA_135520 (aconitase), were identified ( Fig. 4D ). The three recombinant proteins were produced, and Western blotting revealed that only recombinant PbHSP90 is recognized by the anti-MG1 antibody ( Fig. 4E...”
- Stage-Specific Changes in Plasmodium Metabolism Required for Differentiation and Adaptation to Different Host and Vector Environments
Srivastava, PLoS pathogens 2016 - “...Also see S3 and S7 Figs. A P . berghei mutant ( aco - , PBANKA_135520) lacking the TCA cycle enzyme aconitase (ACO), which is responsible for the isomeric conversion of citrate to isocitrate, was generated but exhibited a severe growth defect during asexual development (...”
- Identification of vital and dispensable sulfur utilization factors in the Plasmodium apicoplast
Haussig, PloS one 2014 - “...non-mito 0.5453 PBANKA_130330 PF3D7_1439400 ubiquinol-cytochrome c reductase iron-sulfur subunit, putative /++ no SP possibly 0.9782 PBANKA_135520 PF3D7_1342100 aconitate hydratase /++ no SP possibly 0.8460 PBANKA_142880 PF3D7_1212800 iron-sulfur subunit of succinate dehydrogenase /++ no SP possibly 0.1521 PBANKA_143040 PF3D7_1214600 adrenodoxin-type ferredoxin, putative /++ no SP possibly 0.8299...”
G3V6S2 Cytoplasmic aconitate hydratase from Rattus norvegicus
46% identity, 95% coverage
- The zinc transporter Zip14 (SLC39a14) affects Beta-cell Function: Proteomics, Gene expression, and Insulin secretion studies in INS-1E cells
Maxel, Scientific reports 2019 - “...Transmembrane emp24 domain-containing protein 4 0.85 Numa1 F7FF45 Nuclear mitotic apparatus protein 1 0.85 Aco1 G3V6S2 Cytoplasmic aconitate hydratase 0.85 Slirp D4A4W6 SRA stem-loop-interacting RNA-binding protein, mitochondrial 0.86 Pacs1 F1LPG3 Phosphofurin acidic cluster sorting protein 1 0.86 Strbp D3ZDD7 Spermatid perinuclear RNA binding protein, isoform 0.87...”
- Oxidative Biochemistry Disbalance and Changes on Proteomic Profile in Salivary Glands of Rats Induced by Chronic Exposure to Methylmercury
Bittencourt, Oxidative medicine and cellular longevity 2017 - “...synthase subunit beta, mitocondrial (P10719), were downregulated and malate dehydrogenase, cytoplasmic (O88989), and aconitate hydratase (G3V6S2) were unique in the control group. In submandibular, ATP synthase subunit alpha, mitochondrial (P15999), was downregulated, while ADP/ATP translocase 2 (Q09073), ATP synthase subunit delta, mitochondrial (P35434), citrate synthase (G3V936),...”
AL01_06260 aconitate hydratase AcnA from Bombella intestini
48% identity, 97% coverage
- Whole-Genome Sequence Analysis of Bombella intestini LMG 28161T, a Novel Acetic Acid Bacterium Isolated from the Crop of a Red-Tailed Bumble Bee, Bombus lapidarius
Li, PloS one 2016 - “...decarboxylase (AL01_08375); 19, alcohol dehydrogenase (AL01_01980, AL01_07015); 20, citrate synthase (AL01_06255); 21, aconitate hydratase 1 (AL01_06260); 22, NADP+-dependent isocitrate dehydrogenase (AL01_06250); 23, 2-oxoglutarate dehydrogenase E1 (AL01_08340); 24, dihydrolipoyllysine succinyltransferase (AL01_07740); 25, dihydrolipoamide dehydrogenase (AL01_00930); 26, fumarate hydratase (AL01_05840); 27, PQQ-dependent glucose dehydrogenase (AL01_09305); 28, gluconolactonase (AL01_06230);...”
Q9HBB2 Cytoplasmic aconitate hydratase from Homo sapiens
47% identity, 88% coverage
pmi / A0A0M3N196 phosphinomethylmalate isomerase (EC 4.2.1.166) from Kitasatospora phosalacinea (see 2 papers)
47% identity, 95% coverage
3snpA / Q01059 Crystal structure analysis of iron regulatory protein 1 in complex with ferritin h ire RNA (see paper)
45% identity, 97% coverage
G3HQZ8 Cytoplasmic aconitate hydratase from Cricetulus griseus
45% identity, 95% coverage
EDI29_04400 aconitate hydratase AcnA from Pectobacterium polonicum
44% identity, 97% coverage
pmi / Q5IW57 phosphinomethylmalate isomerase (EC 4.2.1.166) from Streptomyces viridochromogenes (see paper)
PMI_STRVT / D9XF46 Phosphinomethylmalate isomerase; EC 4.2.1.166 from Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) (see paper)
45% identity, 97% coverage
- function: Isomerase involved in the biosynthesis of phosphinothricin tripeptide (PTT), also known as bialaphos (BA), a natural-product antibiotic and potent herbicide (PubMed:11472937). Probably catalyzes the isomerization of phosphinomethylmalate to phosphinomethylisomalate (PubMed:11472937). Shows no standard aconitase activity with citrate as a substrate and is not able to complement an acnA mutant (PubMed:11472937).
catalytic activity: phosphinomethylmalate = phosphinomethylisomalate (RHEA:49504)
catalytic activity: phosphinomethylmalate = 2-(phosphinatomethylidene)butanedioate + H2O (RHEA:49508)
catalytic activity: 2-(phosphinatomethylidene)butanedioate + H2O = phosphinomethylisomalate (RHEA:49512)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
disruption phenotype: Disruption mutant loses the ability to produce PTT, but it shows normal growth behavior and is able to form aerial mycelium and to sporulate (PubMed:11472937). Disruption of the gene does not affect aconitase activity (PubMed:11472937).
ELEN_RS11710 aconitate hydratase AcnA from Eggerthella lenta DSM 2243
44% identity, 98% coverage
Q4JFF1 phosphinomethylmalate isomerase (EC 4.2.1.166) from Streptomyces viridochromogenes (see paper)
44% identity, 97% coverage
FAD_0701 aconitate hydratase AcnA from Ferroplasma acidiphilum
43% identity, 96% coverage
- Metabolic and evolutionary patterns in the extremely acidophilic archaeon Ferroplasma acidiphilum YT
Golyshina, Scientific reports 2017 - “...enzyme/malate dehydrogenase (FAD_0703); 5, pyruvate: ferredoxin oxidoreductase (FAD_05670568); 6, citrate synthase (FAD_1100); 7, aconitate hydratase (FAD_0701); 8, isocitrate dehydrogenase (FAD_1632); 9,2-oxoglutarate:ferredoxin oxidoreductase (FAD_07120713); 10, succinyl-CoA synthetase (FAD_0709710); 11, succinate dehydrogenase (FAD_07140717); 12, fumarate hydratase (FAD_1630); 13, malate dehydrogenase (FAD_0718); 14, glutamate dehydrogenase (FAD_0434); 15, aspartate aminotransferase...”
C0HER4 Aconitate hydratase from Zea mays
48% identity, 77% coverage
- Physiological and comparative proteomic analysis provides new insights into the effects of shade stress in maize (Zea mays L.)
Gao, BMC plant biology 2020 - “...sources [ 46 ]. Alcohol dehydrogenase superfamily protein (K7UAQ8, A0A096SZS3, A0A096TTS5), beta-galactosidase (A0A096R2T2), aconitate hydratase (C0HER4), beta-amylase (A0A0B4J3H2), and other proteins involved in glycolysis and tricarboxylic acid cycle were significantly depressed by shade. Thus, shade destroyed glycolysis, the tricarboxylic acid cycle, and other energy metabolic pathways,...”
- Proteomics analysis reveals a dynamic diurnal pattern of photosynthesis-related pathways in maize leaves
Feng, PloS one 2017 - “...46.61 5.72 B6TQ36 pyruvate dehydrogenase E1 component subunit beta 7 5.05 2 396 42.29 7.02 C0HER4 uncharacterized LOC100304315 7 10.07 2 685 74.27 6.13 C0PFT6 uncharacterized LOC100383412 7 3.07 2 814 92.22 7.18 Carbon fixation in photosynthetic organisms Fifty identified proteins were enzymes involved in carbon...”
- A comparative glycoproteome study of developing endosperm in the hexose-deficient miniature1 (mn1) seed mutant and its wild type Mn1 in maize
Silva-Sanchez, Frontiers in plant science 2014 - “...2 6.39 6 159.25 93.92 0.71 1.12 13 4 3 0.09 Solrzano et al., 2012 C0HER4 Simillar to Putative aconitate hydratase 1 from Sorghum bicolor (Q1KSB0) 5.76 74.31 11 15 3 0.10 641 Q948J8 Uncleaved legumin-1 6.66 6.2 52.33 52.83 0.71 0.88 15 10 1 0.77...”
VC1338 aconitate hydratase 1 from Vibrio cholerae O1 biovar eltor str. N16961
41% identity, 94% coverage
- Metabolic Reprogramming of Vibrio cholerae Impaired in Respiratory NADH Oxidation Is Accompanied by Increased Copper Sensitivity
Toulouse, Journal of bacteriology 2018 - “...cycle and connected reactions A0A0H3AKS4 A0A0H3AMD5 A0A0H3AMM1 A0A0H3AHX2 VC1338 VC0736 VC0734 VC1336 acnA aceA aceB prpB 2.4 2.3 2 2.3 Aconitate hydratase...”
- “...strain, the decreased amount of aconitate hydratase (or aconitase; VC1338, RF of 2.4) is expected to result in diminished NADH formation by the oxidative branch...”
- A novel phase variant of the cholera pathogen shows stress-adaptive cryptic transcriptomic signatures
Lambert, BMC genomics 2016 - “...regulated (Additional file 11 : Table S7). Other TCA/GB genes, including VC2092 ( gltA ), VC1338 ( acnA ), VC0604 ( acnB ) and VC1141 ( icd ), or TCA only genes VC2086 ( sucB ) and VC2085 ( sucC ), were not significantly changed from...”
- Second-generation recombination-based in vivo expression technology for large-scale screening for Vibrio cholerae genes induced during infection of the mouse small intestine
Osorio, Infection and immunity 2005 - “...(trpG; anthranilate synthase component II), VC1338 (acnA; aconitase hydratase 1), VC1137 (hisA; phosphoribosylformimino-5-aminoimidazole carboxamide ribotide...”
- “...VC0874 VC1173 VC0202 VC0203 VC1137 VC1535 VC1275 VC0130 VC1338 VC2621 VCA0632 VC2419 VC1500 VCA0752 VCA0687 VC2705 VC1687 VC2742 VCA0016 VCA0242 VC2646 VC2130...”
- Identification of novel stage-specific genetic requirements through whole genome transcription profiling of Vibrio cholerae biofilm development
Moorthy, Molecular microbiology 2005 - “...VC1075 VC1077 VC1330 VC1332 VC1333 VC1334 VC1335 VC1336 VC1338 VC1339 VC1514 VC1515 VC1516 VC1517 VC1518 VC1519 VC2705 VC2706 VC2708 VC2709 VC2711 VCA0676...”
IREB2_HUMAN / P48200 Iron-responsive element-binding protein 2; IRE-BP 2; Iron regulatory protein 2; IRP2 from Homo sapiens (Human) (see 6 papers)
NP_004127 iron-responsive element-binding protein 2 isoform 1 from Homo sapiens
44% identity, 84% coverage
- function: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit. [4Fe-4S]-binding affects RNA-binding activity, thereby inhibiting activity of the protein.)
subunit: Interacts with RBCK1 isoform 1 and isoform 2 only in iron-rich conditions (PubMed:12629548). Interacts (when associated with the 4Fe- 4S) with FBXL5 (PubMed:19762596, PubMed:19762597). Interacts with CIAO1 and CIAO2A (PubMed:23891004). - Human microRNA (miR-20b-5p) modulates Alzheimer's disease pathways and neuronal function, and a specific polymorphism close to the MIR20B gene influences Alzheimer's biomarkers.
Wang, Molecular psychiatry 2022 - “...+ IL1A P01583 Interleukin-1 alpha Amyloid + IRP1 P21399 Iron-responsive element-binding protein 1 Amyloid IRP2 P48200 Iron-responsive element-binding protein 2 Amyloid MAPK13 O15264 Mitogen-activated protein kinase 13 Tau MAPT P10636 Microtubule-associated protein tau Tau + MME P08473 Membrane metallo-endopeptidase Clearance + PSD95 P78352 Postsynaptic density protein...”
- A Bioinformatics-Assisted Review on Iron Metabolism and Immune System to Identify Potential Biomarkers of Exercise Stress-Induced Immunosuppression.
Bonilla, Biomedicines 2022 - “...(Iron regulatory protein 2) IREB2 (15q25.1) ENSG00000136381 Length: 963 Mass: 105,05 Da (P48200/ SWISS-MODEL Repository P48200) Cytoplasm, mitochondrion. RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3-UTR...”
- Quantitative plasma proteomics of survivor and non-survivor COVID-19 patients admitted to hospital unravels potential prognostic biomarkers and therapeutic targets
Flora, 2021 - Proteomics Reveals that Methylmalonyl-CoA Mutase Modulates Cell Architecture and Increases Susceptibility to Stress
Costanzo, International journal of molecular sciences 2020 - “...DOWN 1.1 L, N P02794 Ferritin heavy chain FTH1 DOWN 0.9 C, ERS, L, N P48200 Iron-responsive element-binding protein 2 IREB2 UP +0.9 C, M Q9NP58 ATP-binding cassette sub-family B member 6, mitochondrial ABCB6 UP +1.4 C, E, ER, ERS, GA, M Q6PML9 Zinc transporter 9...”
- Quantitative Analysis of Ubiquitinated Proteins in Human Pituitary and Pituitary Adenoma Tissues
Qian, Frontiers in endocrinology 2019 - “...1.62E+08 4.52 4.55E-06 P16104 H2AFX Histone H2AX K*TSATVGPK 9 120 1 3.50E+07 1.43E+09 40.96 1.39E-05 P48200 IREB2 Iron-responsive element-binding protein 2 GFQIAAEK*QK* 10 483;485 1; 1 3.46E+07 1.63E+08 4.71 3.31E-05 B2RDW1 RPS30A Epididymis luminal protein 112 TLTGK*TITLEVEPSDTIENVK 21 11 1 1.70E+08 1.26E+09 7.40 3.64E-05 TLSDYNIQK*ESTLHLVLR 18...”
- Application of a hierarchical enzyme classification method reveals the role of gut microbiome in human metabolism
Mohammed, BMC genomics 2015 - “...3.4.24.21* Astacin TASP1 Q9H6P5 3.4.25.- 3.4.25.2* HslU--HslV peptidase ADAL Q6DHV7 3.5.4.- 3.5.4.2* Adenine deaminase IREB2 P48200 None 4.2.1.33* 3-isopropylmalate dehydratase TYRP1 P17643 1.14.18.- 1.14.11.13** Gibberellin 2-beta-dioxygenase HSD11B2 P80365 1.1.1.- 1.3.1.9** Enoyl-[acyl-carrier-protein] reductase (NADH) NDOR1 Q9UHB4 1.6.-.- 1.8.1.2** Sulfite reductase (NADPH) ENDOV Q8N8Q3 3.1.26.- 3.1.21.7** Deoxyribonuclease V...”
- Inactivation of the tricarboxylic acid cycle aconitase gene from Streptomyces viridochromogenes Tü494 impairs morphological and physiological differentiation.
Schwartz, Journal of bacteriology 1999 - KLF14 regulates the growth of hepatocellular carcinoma cells via its modulation of iron homeostasis through the repression of iron-responsive element-binding protein 2.
Zhou, Journal of experimental & clinical cancer research : CR 2023 (PubMed)- GeneRIF: KLF14 regulates the growth of hepatocellular carcinoma cells via its modulation of iron homeostasis through the repression of iron-responsive element-binding protein 2.
- MiR-19a suppresses ferroptosis of colorectal cancer cells by targeting IREB2.
Fan, Bioengineered 2022 - GeneRIF: MiR-19a suppresses ferroptosis of colorectal cancer cells by targeting IREB2.
- Mdm2 is a target and mediator of IRP2 in cell growth control.
Zhang, FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 - GeneRIF: Mdm2 is a target and mediator of IRP2 in cell growth control.
- FBXL5 Regulates IRP2 Stability in Iron Homeostasis via an Oxygen-Responsive [2Fe2S] Cluster.
Wang, Molecular cell 2020 - GeneRIF: This study has identified an iron-sulfur cluster within FBXL5, which promotes IRP2 polyubiquitination and degradation in response to both iron and oxygen concentrations.
- Iron Regulatory Protein 2 Exerts its Oncogenic Activities by Suppressing TAp63 Expression.
Zhang, Molecular cancer research : MCR 2020 - GeneRIF: Iron Regulatory Protein 2 Exerts its Oncogenic Activities by Suppressing TAp63 Expression.
- IRP2-Hif1α/Hif2α signaling: a novel mechanism of metabolic switch from aerobic glycolysis to oxidative phosphorylation.
Zhu, Acta biochimica et biophysica Sinica 2020 (PubMed)- GeneRIF: IRP2-Hif1alpha/Hif2alpha signaling: a novel mechanism of metabolic switch from aerobic glycolysis to oxidative phosphorylation.
- Zinc induces iron uptake and DMT1 expression in Caco-2 cells via a PI3K/IRP2 dependent mechanism.
Kondaiah, The Biochemical journal 2019 (PubMed)- GeneRIF: To better understand the mechanisms of zinc-induced iron absorption, we have studied the effect of zinc on iron uptake, iron transporter and iron regulatory protein (IRP 1 and 2) expression and the impact of the PI3K pathway in differentiated Caco-2 cells, an intestinal cell culture model. We found that zinc induces DMT1 protein and mRNA expression.
- Iron regulatory protein 2 is a suppressor of mutant p53 in tumorigenesis.
Zhang, Oncogene 2019 - GeneRIF: Ectopic expression of IRP2 suppressed cell growth in a mutant p53-dependent manner. Together, these data indicate that mutant p53 gain-of-function can be suppressed by IRP2 and Ferredoxin reductase deficiency, both of which may be explored to target tumors carrying mutant p53.
- More
IREB2_MOUSE / Q811J3 Iron-responsive element-binding protein 2; IRE-BP 2; Iron regulatory protein 2; IRP2 from Mus musculus (Mouse) (see paper)
44% identity, 84% coverage
- function: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit. [4Fe-4S]-binding affects RNA-binding activity, thereby inhibiting activity of the protein.)
subunit: Interacts with RBCK1 only in iron-rich conditions. Interacts (when associated with the 4Fe-4S) with FBXL5 (By similarity). Interacts with CIAO1 and CIAO2A (PubMed:23891004). - Proteomic profile of embryonic stem cells with low survival motor neuron protein is consistent with developmental dysfunction
Parker, Journal of neural transmission (Vienna, Austria : 1996) 2017 - “...TSR2 homolog OS=Mus musculus GN=Tsr2 4.844 Q80TS5 Zinc finger protein 423 OS=Mus musculus GN=Znf423 4.791 Q811J3 Iron-responsive element-binding protein 2 OS=Mus musculus GN=Ireb2 3.951 O70404 Vesicle-associated membrane protein 8 OS=Mus musculus GN=Vamp8 3.487 Q9CQX4 PCNA-associated factor OS=Mus musculus GN=Paf 3.150 P28843 Dipeptidyl peptidase 4 OS=Mus musculus...”
- Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.
Amigó-Correig, PloS one 2012 - “...NADH dehydrogenase L 1.32 0.02 PVN 303 P17751 Triosephosphate isomerase O 1.82 0.023 PVN 567 Q811J3 Aconitase 2 O 1.77 0.034 PVN 359 Q00623 Apolipoprotein A-I O 1.6 0.002 PVN 431 Q04447 Creatine kinase B L 1.14 0.003 ARC 361 P21550 Enolase 3 L 1.21 0.028...”
BAU10_07690 Fe/S-dependent 2-methylisocitrate dehydratase AcnD from Vibrio alginolyticus
42% identity, 94% coverage
VP1646 aconitate hydratase 1 from Vibrio parahaemolyticus RIMD 2210633
VP_RS07925 Fe/S-dependent 2-methylisocitrate dehydratase AcnD from Vibrio parahaemolyticus RIMD 2210633
42% identity, 94% coverage
MIM_c31340 Fe/S-dependent 2-methylisocitrate dehydratase AcnD from Advenella mimigardefordensis DPN7
41% identity, 95% coverage
- Proteomic analysis of organic sulfur compound utilisation in Advenella mimigardefordensis strain DPN7T
Meinert, PloS one 2017 - “...protein; MIM_c31300, hypothetical protein; MIM_c31310, hypothetical protein; MIM_c31320, methylisocitrate lyase; MIM_c31330, putative AcnD-accessory protein PrpF; MIM_c31340, Fe/S-dependent 2-methylisocitrate dehydratase; MIM_c31350, 2-methylcitrate synthase; MIM_c31360, transcriptional regulator, XRE family ; MIM_c31370, transcriptional regulator, LysR family ; MIM_c31380, acyl-CoA transferase, family III; MIM_c31390, desulfinase Acd; MIM_c31400, 3-mercaptopropionate dioxygenase Mdo;...”
H16_A1907 Methyl-cis-aconitic acid hydratase/Aconitate hydratase from Ralstonia eutropha H16
41% identity, 95% coverage
- A novel A3 group aconitase tolerates oxidation and nitric oxide
Doi, The Journal of biological chemistry 2015 - “...ECDH10B_1393 PA1562 44 3 40 1 40 2 33 2 AcnA4 Daci_3587 PA0794 H16_A1907 27 2 25 1 36 1 33 3 25 1 26 1 31 1 25 1 AcnB ECDH10B_0098 PA1787 H16_B0568 0.6 0.1 1.4...”
- “...AcnB (H16_B0568) R. eutropha NBRC 102504 AcnM (H16_A1907) S. japonicum UT26S AcnA (SJA_C1-23180) Acn expression plasmids for A. denitrificans pNS-AcnA3 pNSGroE2...”
ACNA_CUPNE / Q937N8 Aconitate hydratase A; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha) (see paper)
41% identity, 94% coverage
- function: Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and the 2- methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate (PubMed:11495997). Could catalyze the hydration of 2-methyl-cis- aconitate to yield (2S,3R)-2-methylisocitrate. The apo form of AcnA functions as a RNA-binding regulatory protein (By similarity).
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
catalytic activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis- aconitate + H2O (RHEA:17941)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.)
subunit: Monomer.
ACIAD2755 aconitate hydratase 1 from Acinetobacter sp. ADP1
42% identity, 95% coverage
Smlt3608 putative aconitate hydratase from Stenotrophomonas maltophilia K279a
41% identity, 95% coverage
- Acquired resistance of Stenotrophomonas maltophilia to antimicrobials induced by herbicide paraquat dichloride
Vanitshavit, PloS one 2024 - “...BT4607 CACGCGAAGGGCGACTGC Reverse primer for sodC2 SOD Cloning of acnA The putative acnA gene ( smlt3608 ) encoding aconitase A was PCR amplified from S . maltophilia K279a genomic DNA with the primers BT8277 and BT8278 using Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific). A 2,650...”
- “...family), smlt0009 (periplasmic protein TonB), smlt0895 (putative 50S ribosomal subunit protein L1, rplA ), and smlt3608 (putative aconitase A, acnA ). Stop-gained or nonsense mutations were detected in smlt3910 (putative glycine betaine transporter 2, opuD2 ). An inframe insertion mutation was identified in smlt0570 (putative hybrid...”
Hsero_2283 Fe/S-dependent 2-methylisocitrate dehydratase AcnD from Herbaspirillum seropedicae SmR1
41% identity, 95% coverage
PA14_53970 probable aconitate hydratase from Pseudomonas aeruginosa UCBPP-PA14
41% identity, 95% coverage
- Gene Expression Profiling of Pseudomonas aeruginosa Upon Exposure to Colistin and Tobramycin
Cianciulli, Frontiers in microbiology 2021 - “...16.47 5.26 sucB PA14_44000 Dihydrolipoamide succinyltransferase 10.98 9.15 sucA PA14_44010 2-oxoglutarate dehydrogenase E1 4.27 4.12 PA14_53970 PA14_53970 Aconitate hydratase 18.05 8.09 Phenylalanine/Tyrosine catabolism fahA PA14_38530 Fumarylacetoacetase 30.98 2.32 maiA PA14_38550 Maleylacetoacetate isomerase 48.44 3.57 phhB PA14_53000 Pterin-4-alpha-carbinolamine dehydratase 14.76 2.47 phhC PA14_53010 Aromatic amino acid aminotransferase...”
- “...catalytic activities of the isocitrate dehydrogenase Idh, the dihydrolipoamide succinyltransferase SucB and the aconitate hydratase PA14_53970 result in an increased NADH content and promote cellular respiration ( Kohanski et al., 2007 ; Meylan et al., 2017 ). Upon tobramycin treatment, a down-regulation was observed for these...”
- Genomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa
Dötsch, Antimicrobial agents and chemotherapy 2009 - “...PA14_05540 PA14_07430 PA14_07770 PA14_54410 PA14_54330 PA14_53970 PA14_53000 PA14_52260 PA14_51780 PA14_51320 PA14_49320 PA14_47230 PA14_41570 PA14_40620...”
PA0794 probable aconitate hydratase from Pseudomonas aeruginosa PAO1
41% identity, 95% coverage
- PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in Pseudomonas aeruginosa
Cui, Science advances 2022 - “...60-fold) up-regulated genes (table S1). The database of P. aeruginosa genome showed that PA0793 and PA0794 share the same operon; it is likely that prpB , methylisocitrate lyase, is cotranscribed with pmiR (fig. S2A) ( 2 ). Moreover, it is predicted that these clusters encode enzymes...”
- The Human Innate Immune Protein Calprotectin Elicits a Multimetal Starvation Response in Pseudomonas aeruginosa
Nelson, Microbiology spectrum 2021 - “...PAO1). Specifically, Fe limitation resulted in the downregulation of Fe-containing TCA cycle enzymes (SdhBAC, AcnA, PA0794, and PA4330), a putative bacterioferritin (PA4880), and Fe-containing oxidative stress response proteins (SodB and KatA), the upregulation of Fe-independent homologs of TCA cycle enzymes (MqoA and FumC1) and a superoxide...”
- Mobilization of Iron Stored in Bacterioferritin Is Required for Metabolic Homeostasis in Pseudomonas aeruginosa
Punchi, Pathogens (Basel, Switzerland) 2020 - “...81 ] are depleted in the bfd cells. In addition, the aconitase enzymes AcnA and PA0794, which depend on [4Fe-4S] catalytic centers to transform citrate to isocitrate via cis-aconitate, are also depleted in the mutant cells. In the bottom section of the TCA cycle, the conversion...”
- “...and Lys may also be impacted by depletion of the iron-dependent aconitase enzymes AcnA and PA0794, required to transform citrate to isocitrate. We used NMR spectroscopy (see Materials and Methods) to identify and measure the relative concentrations of several of these amino acids in the wt...”
- Proteomic Analysis of the Pseudomonas aeruginosa Iron Starvation Response Reveals PrrF Small Regulatory RNA-Dependent Iron Regulation of Twitching Motility, Amino Acid Metabolism, and Zinc Homeostasis Proteins
Nelson, Journal of bacteriology 2019 - “...complementarity with the PA4333 (C), sdhC (D), and PA0794 (E) mRNAs was identified in previous CopraRNA analyses (63). metabolism to utilize the glyoxylate...”
- “...mRNAs encoding the TCA cycle enzymes SdhC, PA4333, and PA0794 (Fig. 1C to E), none of which were identified as sharing complementarity with the PrrF sRNAs in...”
- CFTR-PTEN-dependent mitochondrial metabolic dysfunction promotes Pseudomonas aeruginosa airway infection
Riquelme, Science translational medicine 2019 - “...( zwf and glk ), glyoxylate shunt ( aceA and glcB ), and TCA cycle (PA0794, idh , sucA , and fumC1 ), as well as the cyclic di-guanosine monophosphate (cdi-GMP) cascade, the master regulator of biofilm production. Representative isolates (small colony variant 686 and mucoid...”
- Within-Host Evolution of the Dutch High-Prevalent Pseudomonas aeruginosa Clone ST406 during Chronic Colonization of a Patient with Cystic Fibrosis
van, PloS one 2016 - “...30 PA4163 Translation, ribosomal structure and biogenesis [ 13 ] 0807 GCC[A] > ACC[T] 703 PA0794 Energy production and conversion [ 13 ] 3973 GCC[A] > GGC[G] 61 PA3280 oprO Inorganic ion transport and metabolism [ 13 ] 5588 GCC[A] > GTC[V] 489 PA4266 b fusA1...”
- A novel A3 group aconitase tolerates oxidation and nitric oxide
Doi, The Journal of biological chemistry 2015 - “...4 ECDH10B_1393 PA1562 44 3 40 1 40 2 33 2 AcnA4 Daci_3587 PA0794 H16_A1907 27 2 25 1 36 1 33 3 25 1 26 1 31 1 25 1 AcnB ECDH10B_0098 PA1787 H16_B0568 0.6 0.1...”
- “...pAcnB EC0098 pET28a (+) pAcnA OA0105 pET28a (+) pAcnA PA0794 pET28a (+) pAcnA PA1562 pET28a (+) pAcnB PA1787 pET28a (+) pAcnA REA2638 pET28a (+) pAcnB REB0568...”
- Coexistence and within-host evolution of diversified lineages of hypermutable Pseudomonas aeruginosa in long-term cystic fibrosis infections
Feliziani, PLoS genetics 2014 - “...metabolism 5 1 4 PA4285 recC DNA replication, recombination, modification and repair 5 2 3 PA0794 Energy metabolism 5 2 3 PA1613 Membrane proteins 6 4 2 PA2072 5 3 2 PA3234 Membrane proteins; Transport of small molecules 5 2 3 PA3920 5 1 4 PA4719...”
- “...related to bacterial catabolism ( e.g. , aceE , gcvP1 , soxA , xdhB , PA0794) were also observed, suggesting that the inactivation of certain metabolic functions may be a common trait related to CF host adaptation (see below). The concurrent alteration of specific genes or...”
- More
NMA2052 aconitate hydratase from Neisseria meningitidis Z2491
41% identity, 97% coverage
NMB0433 aconitate hydratase from Neisseria meningitidis MC58
41% identity, 97% coverage
- A large genomic island allows Neisseria meningitidis to utilize propionic acid, with implications for colonization of the human nasopharynx
Catenazzi, Molecular microbiology 2014 - “...., 2003 ) and methylcitrate synthase ( prpC ) (Horswill and Escalante-Semerena, 1999a ) respectively. NMB0433 and NMB0434 are homologues of acnD and prpF from Shewanella oneidensis and Vibrio cholerae that catalyse methylcitrate dehydratase activity, acting as a functional replacement for the gene prpD that is...”
- “...the different propionic acid utilization patterns in different media. Genes in the prp cluster NMB0430, NMB0433 and NMB0434 are highly similar to genes required for methylcitrate cycle action in other bacteria, but NMB0432 and NMB0435 are not similar to methylcitrate cycle genes from other organisms. To...”
- Analysis of the regulated transcriptome of Neisseria meningitidis in human blood using a tiling array
Del, Journal of bacteriology 2012 - “...asRNA to NMB0432, which seems to be cotranscribed with NMB0433. The same was observed for the asRNA to putA (NMB0401) and exbB (NMB1729) (Fig. 3B)....”
- Environmental adaptability and stress tolerance of Laribacter hongkongensis: a genome-wide analysis
Lau, Cell & bioscience 2011 - “...LHK_00495 CV1120 NMB1458 NGO1029 acnA Aconitase A SoxRS, FNR, Fur, RpoS LHK_02153 LHK_02309 CV1121 CV2054 NMB0433 - Superoxide scavenging sodA Manganese superoxide dismutase SoxRS, FNR - - - - sodB Iron superoxide dismutase LHK_01716 CV0867 CV2504 NMB0884 NGO0405 sodC Copper-zinc superoxide dismutase H 2 O 2...”
VT47_09995 Fe/S-dependent 2-methylisocitrate dehydratase AcnD from Pseudomonas syringae pv. syringae
40% identity, 95% coverage
GY20_RS0107915 aconitate hydratase from Corynebacterium glutamicum
46% identity, 70% coverage
- Copper Resistance Mechanism and Copper Response Genes in Corynebacterium crenatum
Huang, Microorganisms 2024 - “...metabolism, which included three succinate dehydrogenase genes (GY20_RS0101900, GY20_RS0101245, GY20_RS0101250) and one aconitate hydratase gene (GY20_RS0107915), were selected to conduct the qRT-PCR. The log2 FC of these DEGs ranged between 1.2 and 1.5 ( Table S3 ). The qRT-PCR results showed that the expression levels of...”
- “...metabolism. ( A ) qRT-PCR of DEGs related to energy metabolism. GY20_RS0101900, GY20_RS0101245, GY20_RS0101250, and GY20_RS0107915 encode succinate dehydrogenase/fumarate reductase iron-sulfur subunit, succinate dehydrogenase cytochrome b subunit, succinate dehydrogenase (quinone) flavoprotein subunit, and aconitate hydratase, respectively. ( B ) Concentrations of ATP, NADP+, and NADPH. *...”
BUE60_12810 Fe/S-dependent 2-methylisocitrate dehydratase AcnD from Pseudomonas syringae pv. actinidiae
40% identity, 95% coverage
PP_2336 Fe/S-dependent 2-methylisocitrate dehydratase AcnD from Pseudomonas putida KT2440
PP2336 aconitate hydratase, putative from Pseudomonas putida KT2440
40% identity, 95% coverage
- Computational analysis into the potential of azo dyes as a feedstock for actinorhodin biosynthesis in Pseudomonas putida
Nayyara, PloS one 2024 - “...MICITDr, GLYOX, ACACT11, MALS, PGL, ACONTa, NACODA PP_2492, PP_0327, PP_1367, PP_1943, PP_2589, PP_0545, PP_2694, PP_0654, PP_2336, PP_4144, PP_0356, PP_1023, PP_2112, PP_2339, PP_5186, PP_2137, PP_2215 Our results highlight that the number of possible growth-coupling regimes and the minimum product yield allowable through growth-coupling is dependent on the...”
- Fatty Acid and Alcohol Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing
Thompson, Applied and environmental microbiology 2020 (secret) - A metabolic pathway for catabolizing levulinic acid in bacteria
Rand, Nature microbiology 2017 - “...methyl ester esterase PP_0988 1128706 gcvP-1 glycine dehydrogenase PP_2332 2660666 N/A ATP-dependent zinc protease family PP_2336 2666405 acnA-II aconitate hydratase PP_2337 2666944 prpF aconitate isomerase PP_2791 3181098 N/A Phosphotransferase family PP_2793 3182533 N/A acyl-CoA dehydrogenase family protein PP_2794 3183601 N/A short chain dehydrogenase/reductase family PP_3741 4271628...”
- Genome-scale reconstruction and analysis of the Pseudomonas putida KT2440 metabolic network facilitates applications in biotechnology
Puchałka, PLoS computational biology 2008 - “...immediately adjacent to the ORF PP2330. Inspection of this region of the genome revealed that PP2336 is annotated as aconitate hydratase, putative, although the flanking genes are responsible for degradation of propanoate. Analysis of PP2330 via BLAST revealed a homology of more than 99% over the...”
- “...family protein 5-Aminopentanamidase; EC 3.5.1.30 [36] PP0383 Tryptophan 2-monooxygenase, putative Lysine 2-monooxygenase; EC 1.13.12.2 [36] PP2336 Aconitate hydratase, putative; EC:4.2.1.3 2-Methylisocitrate dehydratase; EC 4.2.1.99 a PP2432 Oxygen-insensitive NAD(P)H nitroreductase; EC:1.-.-.- 6,7-Dihydropteridine reductase; EC 1.5.1.34 a PP3591 Malate dehydrogenase, putative; EC:1.1.1.37 1 -Piperideine-2-carboxylate reductase; EC 1.5.1.21 [36]...”
FQU82_00161 Fe/S-dependent 2-methylisocitrate dehydratase AcnD from Acinetobacter baumannii
ACICU_00101 aconitase A from Acinetobacter baumannii ACICU
40% identity, 95% coverage
- Transcriptomic analysis reveals the regulatory role of quorum sensing in the Acinetobacter baumannii ATCC 19606 via RNA-seq
Xiong, BMC microbiology 2022 - “...FQU82_00192, FQU82_00189 ( mmsA ), FQU82_00193, FQU82_00159 ( prpB ), FQU82_00160 ( prpC ), and FQU82_00161 ( acnD ). In purine metabolism, 8 DEGs were downregulated, including FQU82_00576 ( ndk ), FQU82_02944 ( purL ), FQU82_02508 ( purH ), FQU82_02833 ( purB ), FQU82_00036, FQU82_01050 (...”
- Phylomark, a tool to identify conserved phylogenetic markers from whole-genome alignments
Sahl, Applied and environmental microbiology 2012 - “...mark (associated with locus tags ACICU_00263, ACICU_03025, and ACICU_00101) outperformed the PubMLST markers in terms of RF values (see Table S2 in the...”
RSUY_RS17195 aconitate hydratase AcnA from Ralstonia solanacearum
40% identity, 94% coverage
XCV1158 aconitate hydratase from Xanthomonas campestris pv. vesicatoria str. 85-10
39% identity, 95% coverage
- Aconitase B is required for optimal growth of Xanthomonas campestris pv. vesicatoria in pepper plants
Kirchberg, PloS one 2012 - “...rpfA [23] , is divergently transcribed from the acnB gene while a second acnA2 gene (XCV1158) is located at a separate location on the genome in a cluster of genes predicted to encode enzymes of methylcitrate metabolism [24] . In this study we examined the potential...”
- “...levels. As a further control we analyzed the transcript levels of a second acnA gene (XCV1158), termed acnA2 , which is located elsewhere on the chromosome of Xcv , and which encodes a predicted methylcitrate dehydratase that showed similar levels of transcript in both exponential and...”
BOW65_RS08625 Fe/S-dependent 2-methylisocitrate dehydratase AcnD from Pseudomonas koreensis
39% identity, 95% coverage
PFLU4630 aconitate hydratase from Pseudomonas fluorescens SBW25
39% identity, 95% coverage
O04916 Aconitate hydratase, cytoplasmic (Fragment) from Solanum tuberosum
46% identity, 70% coverage
XP_006511398 iron-responsive element-binding protein 2 isoform X1 from Mus musculus
48% identity, 51% coverage
LLKF_0647 aconitate hydratase from Lactococcus lactis subsp. lactis KF147
50% identity, 50% coverage
- Strain-Dependent Transcriptome Signatures for Robustness in Lactococcus lactis
Dijkstra, PloS one 2016 - “...riboflavin synthase subunit beta positive 0.2 LLKF_0570 yfiE organic hydroperoxide resistance family protein positive 24.3 LLKF_0647 citB aconitate hydratase negative 0.3 LLKF_0471 ligA NAD-dependent DNA ligase positive 2.9 LLKF_0215 yqeL GTP-binding protein positive 2.0 LLKF_0151 ybgA hypothetical protein negative 0.3 LLKF_2444 pp401 phage integrase positive 2.4...”
llmg_0636 aconitate hydratase from Lactococcus lactis subsp. cremoris MG1363
49% identity, 50% coverage
- Transcriptional response of Lactococcus lactis during bacterial emulsification
Tarazanova, PloS one 2019 - “...of Emul10/Susp10 p -value C. Energy production and conversion llmg_0635 gltA citrate synthase 6.5 8.1e-4 llmg_0636 citB aconitate hydratase 13.9 6.9e-8 llmg_0637 icd isocitrate dehydrogenase 11.3 4.7e-9 E. Amino acid transport and metabolism llmg_0362 dppA dipeptide-binding protein precursor 45.3 1.9e-7 llmg_pseudo_09 dppP dipeptide-binding protein 4.6 1.1e-6...”
Rmet_4240 aconitate hydratase AcnA from Cupriavidus metallidurans CH34
Rmet_4240 aconitate hydratase 1 from Ralstonia metallidurans CH34
48% identity, 55% coverage
- The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments
Janssen, PloS one 2010 - “...(Rmet_2481, Rmet_4144, Rmet_4268, Rmet_4946, and Rmet_5380) and citrate to D-isocitrate via aconitase AcnA (Rmet_1585, Rmet_2492, Rmet_4240, and Rmet_5296). We note that D-isocitrate, as for full TCA cycles, is transfigured into 2-ketoglutarate by isocitrate dehydrogenase ICDH (Rmet_2895, Rmet_3729). The carbon skeleton of 2-ketoglutarate then is used, depending...”
C4J6M5 Aconitate hydratase 3 mitochondrial from Zea mays
41% identity, 64% coverage
ST0833 855aa long hypothetical aconitate hydratase from Sulfolobus tokodaii str. 7
47% identity, 51% coverage
SiRe_1005 aconitate hydratase AcnA from Sulfolobus islandicus REY15A
48% identity, 51% coverage
acnD / Q8EJW3 2-methylcitrate dehydratase (EC 4.2.1.117) from Shewanella oneidensis (strain MR-1) (see paper)
ACND_SHEON / Q8EJW3 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming); Aconitate hydratase; ACN; Aconitase; EC 4.2.1.117; EC 4.2.1.3 from Shewanella oneidensis (strain ATCC 700550 / JCM 31522 / CIP 106686 / LMG 19005 / NCIMB 14063 / MR-1) (see paper)
Q8EJW3 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (EC 4.2.1.117) from Shewanella oneidensis (see paper)
SO0343 aconitate hydratase 1 from Shewanella oneidensis MR-1
45% identity, 52% coverage
- function: Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle II (propionate degradation route). In vivo under anaerobic conditions, AcnD catalyzes the stereospecific dehydration of (2S,3S)-methylcitrate (2-MC) to yield the trans isomer of 2-methyl-aconitate (2-MCA). AcnD can also accept citrate and cis- aconitate, but with a lower efficiency. 2-methylisocitrate and isocitrate are not substrates.
catalytic activity: (2S,3S)-2-methylcitrate = 2-methyl-trans-aconitate + H2O (RHEA:26522)
catalytic activity: citrate = D-threo-isocitrate (RHEA:10336)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster per subunit.) - Genome-scale metabolic network validation of Shewanella oneidensis using transposon insertion frequency analysis
Yang, PLoS computational biology 2014 - “...protein activity regulation simulation in metabolic models. In the MR-1 model, acnB (SO0432) and acnD (SO0343) were independently assigned to the aconitase reaction (OR relationship), resulting in a nonessential prediction for acnB . However, an acnB deletion strain was unable to grow in the presence of...”
- An empirical strategy for characterizing bacterial proteomes across species in the absence of genomic sequences
Turse, PloS one 2010 - “...eno 8 3 2 2 SO3547 pgi 3 SO3991 fbp 4 3 2 TCA Cycle SO0343 acnA 5 3 SO0344 prpC 4 SO0432 acnB 30 10 3 SO0770 mdh 10 7 5 2 SO0970 SO0970 11 4 SO1484 aceA 2 SO1926 gltA 6 4 2 SO1928...”
- “...2 2 SO3991 CN32_0676 fbp 5 3 5 4 4 3 3 4 TCA Cycle SO0343 CN32_3646 acn A 4 4 5 3 4 4 4 5 SO0344 CN32_3645 prp C 2 2 3 3 2 3 2 3 SO0432 CN32_3409 acn B 19 13 19...”
- Snapshot of iron response in Shewanella oneidensis by gene network reconstruction
Yang, BMC genomics 2009 - “...0.39 0.35 0.36 0.29 0.44 0.26 2.68 2.51 3.68 6.47 4.22 6.00 Aerobic energy metabolism SO0343 2-methyl citrate dehydratase (AcnD) 1.19 0.99 0.86 2.23 3.53 8.22 1.14 0.86 0.47 0.21 0.25 0.34 SO0344 methylcitrate synthase (PrpC) 0.81 0.61 0.52 1.77 4.17 8.74 0.90 0.63 0.30 0.11...”
- Probing regulon of ArcA in Shewanella oneidensis MR-1 by integrated genomic analyses
Gao, BMC genomics 2008 - “...complex so0266-9 (ccmF-1) 1.07 0.94 H 71 + 2.63 GTGAACAGAATGTTA Cytochrome c-type biogenesis protein CcmF so0343 (acnA) 2.35 0.08 H 53 + 2.31 CTTAACTCAATGTGC Aconitate hydratase 1 so0383-2 (hsdSM) -0.64 -0.59 J 276 + 3.04 GTTAATAAAATGTTT Type I restriction-modification system so0756 (aroG) -0.84 -2.18 A 164...”
- Global transcriptome analysis of Shewanella oneidensis MR-1 exposed to different terminal electron acceptors
Beliaev, Journal of bacteriology 2005 - “...mRNA levels of genes encoding TCA cycle enzymes (SO0343, SO1930 to 1933, SO2629) cannot be readily explained. It seems likely however, that simultaneous...”
A1S_0076 aconitate hydratase from Acinetobacter baumannii ATCC 17978
46% identity, 52% coverage
ABO_1431 aconitate hydratase, putative from Alcanivorax borkumensis SK2
44% identity, 50% coverage
- An impaired metabolic response to hydrostatic pressure explains Alcanivorax borkumensis recorded distribution in the deep marine water column
Scoma, Scientific reports 2016 - “...= 0.08 169.1 160.3 1864 ABO_1501 type II citrate synthase = 0.12 82.3 89.3 1152 ABO_1431 aconitate hydratase 1.27 321.6 778.2 872 ABO_0694 aconitate hydratase TCA cycle + 1.98 224.4 56.9 1871 ABO_1201 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase + 1.15 186.7 84.2 1441 ABO_0296 isocitrate dehydrogenase...”
- “...dehydrogenase component = 0.11 121.7 131.6 1539 ABO_2749 fumarate hydratase = 0.12 82.3 89.3 1152 ABO_1431 aconitate hydratase = 0.48 182.4 253.9 1044 ABO_0622 pyruvate dehydrogenase subunit E1 = 0.5 110.3 156.3 1267 ABO_1282 isocitrate dehydrogenase 0.56 346.8 512.4 1237 ABO_1500 succinate dehydrogenase, cytochrome b556 subunit...”
Bcep1808_3678 aconitate hydratase from Burkholderia vietnamiensis G4
A4JK60 2-methylcitrate dehydratase (Trans-methylaconitate-forming) from Burkholderia vietnamiensis (strain G4 / LMG 22486)
46% identity, 50% coverage
- Differential Expression and PAH Degradation: What Burkholderia vietnamiensis G4 Can Tell Us?
Cauduro, International journal of microbiology 2020 - “...Bcep1808_0100 109204..110988(+) 1785 ABC transporter-like protein Yes A4JQ98 Bcep1808_5507 63088..64278(+) 1191 Acetyl-CoA acetyltransferase Yes A4JK60 Bcep1808_3678 423951..426545(-) 2595 Aconitate hydratase Yes A4JJ73 Bcep1808_3337 29534..30667() 1134 Acyl-CoA dehydrogenase Yes A4JTS0 Bcep1808_6786 182448..183836() 1389 Aldehyde dehydrogenase Yes A4JKD6 Bcep1808_3756 501291..502235(+) 945 aldo/keto reductase Yes A4JJ72 Bcep1808_3336 27598..29310(-) 1713...”
- Differential Expression and PAH Degradation: What Burkholderia vietnamiensis G4 Can Tell Us?
Cauduro, International journal of microbiology 2020 - “...A4JA20 Bcep1808_0100 109204..110988(+) 1785 ABC transporter-like protein Yes A4JQ98 Bcep1808_5507 63088..64278(+) 1191 Acetyl-CoA acetyltransferase Yes A4JK60 Bcep1808_3678 423951..426545(-) 2595 Aconitate hydratase Yes A4JJ73 Bcep1808_3337 29534..30667() 1134 Acyl-CoA dehydrogenase Yes A4JTS0 Bcep1808_6786 182448..183836() 1389 Aldehyde dehydrogenase Yes A4JKD6 Bcep1808_3756 501291..502235(+) 945 aldo/keto reductase Yes A4JJ72 Bcep1808_3336 27598..29310(-)...”
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 784,739 different protein sequences to 1,253,012 scientific articles. Searches against EuropePMC were last performed on November 25 2024.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory