SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 14152 FitnessBrowser__Keio:14152 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q6LX42 Sodium/alanine symporter AgcS; Alanine permease; Alanine/glycine:cation symporter from Methanococcus maripaludis (strain S2 / LL) (see paper)
40% identity, 93% coverage: 3:446/476 of query aligns to 2:449/453 of Q6LX42

query
sites
Q6LX42

D

D

F

F

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T75 (≠ R71) binding ; binding
G79 (= G75) binding
N80 (= N76) binding ; mutation to A: Markedly reduces L-alanine uptake; when associated with A-274 and A-308.
I165 (= I157) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
Q170 (= Q162) binding ; binding
F273 (= F269) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
FS 273:274 (= FS 269:270) binding
FSNE 273:276 (= FSNE 269:272) binding
S274 (= S270) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-308.
D308 (= D306) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-274.
W370 (≠ N367) mutation to Q: Does not change uptake of L-alanine, but reduces the ability to transport D-alanine.

6csfM Crystal structure of sodium/alanine symporter agcs with d-alanine bound (see paper)
40% identity, 91% coverage: 13:446/476 of query aligns to 1:429/431 of 6csfM

query
sites
6csfM

W

W

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binding d-alanine: T58 (≠ R71), G60 (= G73), G62 (= G75), N63 (= N76), Q150 (= Q162), F253 (= F269), S254 (= S270)
binding sodium ion: T58 (≠ R71), I59 (≠ V72), N63 (= N76), S254 (= S270), D288 (= D306)

6cseM Crystal structure of sodium/alanine symporter agcs with l-alanine bound (see paper)
40% identity, 91% coverage: 13:446/476 of query aligns to 1:429/431 of 6cseM

query
sites
6cseM

W

W

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F

R

D

V

A

L

L

M

M

G

-

S

P

W

M

G

G

A

G

E

L

F

I

V

V

T

T

L

I

V

G

V

L

I

V

L

F

F

F

A

A

F

Y

S

S

S

T

I

V

V

L

A

G

N

W

Y

S

I

Y

Y

Y

A

G

E

E

N

K

N

C

L

-

F

-

F

F

L

E

R

Y

L

L

N

I

N

G

P

T

K

K

A

G

I

I

W

R

C

L

L

Y

R

R

I

I

C

A

T

F

F

V

A

L

T

V

V

A

I

F

G

W

G

G

T

A

L

T

L

A

S

S

L

L

P

P

L

L

M

V

W

W

Q

N

L

I

A

A

D

D

I

T

I

L

M

N

A

G

C

A

M

M

A

A

I

I

T

P

N

N

L

L

T

I

A

G

I

L

L

L

L

L

L

L

S

S

P

G

V

V

V

V

-

V

-

S

H

E

T

T

I

K

A

A

S

F

D

N

Y

E

L

I

R

R

Q

K

R

N

K

E

binding alanine: A57 (= A70), T58 (≠ R71), G60 (= G73), N63 (= N76), Q150 (= Q162), F253 (= F269), S254 (= S270), E256 (= E272)
binding sodium ion: T58 (≠ R71), I59 (≠ V72), N63 (= N76), S254 (= S270), D288 (= D306)

Query Sequence

>14152 FitnessBrowser__Keio:14152
MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFGKSLKNSIHPQPGGLTS
FQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIGMATSFAECSLAQLYKERD
VNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSGVQANAVARALSFSFDFPPLV
TGIILAVFTLLAITRGLHGVARLMQGFVPLMAIIWVLTSLVICVMNIGQLPHVIWSIFES
AFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQM
IGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVILFA
FSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIMACMA
ITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDVSQE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory