SitesBLAST

7lnuB Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to isopentenyl monophosphate and atp (see paper)
27% identity, 59% coverage: 4:220/367 of query aligns to 2:221/261 of 7lnuB

query
sites
7lnuB

S

S

Q

H

T

M

L

I

V

L

V

I

K
|
K

L

L

G

G

T

G

S
|
S

V

V

L

I

T

T

G

D

G

K

S

S

R

E

-

Y

-

H

R

K

L

F

N

N

R

K

A

E

H

T

I

V

V

S

E

R

L

L

-

A

-

D

-

E

V

I

R

R

Q

R

C

S

A

G

Q

Q

-

D

-

V

-

M

-

V

L

V

H

H

A

G

A
|
A

G

G

-

S

-

F

-
x
G

H
|
H

R

-

I

-

V

V

I

I

V

A

T

K

S

K

G

Y

A

A

I

I

A

Q

A

D

G

G

R

-

E

-

H

H

L

V

G

D

Y

D

P

G

E

Q

L

I

P

P

A

A

T

-

I

-

A

A

S

A

K

R

Q

I

L

M

L

C

A

D

A

T

V

R

G

E

Q

L

S

S

R

S

L

M

I

V

-

V

-

E

Q

E

L

L

W

L

E

A

Q

Q

L

G

F

I

S

P

I

A

Y

V

G

S

I

V

H

A

V

P

G

G

Q

S

M

C

L

F

L

V

T

-

R

-

A

-

D

-

M

M

E

E

D

D

R

G

E

K

R

L

F

I

L

V

N

D

A

N

R

E

D

E

T

P

L

I

R

R

A

R

L

L

L

A

D

D

N

L

N

G

I

I

V

M

P

P

V

V

I

M

N

F

E

-

N

G

D

D

A

V

V

V

A

P

T

D

A

R

E

K

-

K

-

G

I

F

K

A

V
x
I

G

V

D
x
S

N
x
G

D

D

N

Q

L

C

S

M

A

E

L

V

A

L

A

C

I

R

L

M

A

F

G

D

A

P

D

E

K

K

L

V

L

F

L

V

T
x
S

D
|
D

Q
x
I

K

D

G

G

L

L

Y
|
Y

T

T

A
|
A

D

D

P

P

R
x
K

S

T

N

D

P

K

Q

K

A

A

E

R

L

L

I

I

K

G

D

E

V

V

-

T

-

R

Y

K

G

K

I

L

D

D

D

E

A

A

L

L

R

T

A

D

I

I

A

T

G

V

D

A

S

D

V

V

S

-

G

-

L

-

G

-

T

T

G

G

M

V

S

H

T

S

K
|
K

L

M

Q

E

A

A

binding adenosine-5'-diphosphate: K8 (= K10), S12 (= S14), S172 (≠ T169), D173 (= D170), I174 (≠ Q171), Y178 (= Y175), A180 (= A177), K183 (≠ R180), K218 (= K217)
binding Isopentenyl phosphate: A48 (= A41), G52 (vs. gap), H53 (= H43), I149 (≠ V146), S151 (≠ D148), G152 (≠ N149)

7lntB Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to benzyl monophosphate and atp (see paper)
27% identity, 59% coverage: 4:220/367 of query aligns to 1:220/260 of 7lntB

query
sites
7lntB

S

S

Q

H

T

M

L

I

V

L

V

I

K

K

L

L

G
|
G

T
x
G

S
|
S

V

V

L

I

T

T

G

D

G

K

S

S

R

E

-

Y

-

H

R

K

L

F

N

N

R

K

A

E

H

T

I

V

V

S

E

R

L

L

-

A

-

D

-

E

V

I

R

R

Q

R

C

S

A

G

Q

Q

-

D

-

V

-

M

-

V

L

V

H

H

A

G

A
|
A

G

G

-

S

-

F

-
x
G

H
|
H

R

-

I

-

V

V

I

I

V

A

T

K

S

K

G

Y

A

A

I

I

A

Q

A

D

G

G

R

-

E

-

H

H

L

V

G

D

Y

D

P

G

E

Q

L

I

P

P

A

A

T

-

I

-

A

A

S

A

K

R

Q

I

L

M

L

C

A

D

A

T

V

R

G

E

Q

L

S

S

R

S

L

M

I

V

-

V

-

E

Q

E

L

L

W

L

E

A

Q

Q

L

G

F

I

S

P

I

A

Y

V

G

S

I

V

H

A

V

P

G

G

Q

S

M

C

L

F

L

V

T

-

R

-

A

-

D

-

M

M

E

E

D

D

R

G

E

K

R

L

F

I

L

V

N

D

A

N

R

E

D

E

T

P

L

I

R

R

A

R

L

L

L

A

D

D

N

L

N

G

I

I

V

M

P

P

V

V

I

M

N

F

E

-

N

G

D

D

A

V

V

V

A

P

T

D

A

R

E

K

-

K

-

G

I

F

K

A

V
x
I

G

V

D
x
S

N

G

D

D

N

Q

L

C

S

M

A

E

L

V

A

L

A

C

I

R

L

M

A

F

G

D

A

P

D

E

K

K

L

V

L

F

L

V

T

S

D
|
D

Q
x
I

K

D

G

G

L

L

Y
|
Y

T

T

A

A

D
|
D

P

P

R

K

S

T

N

D

P

K

Q

K

A

A

E

R

L

L

I

I

K

G

D

E

V

V

-

T

-

R

Y

K

G

K

I

L

D

D

D

E

A

A

L

L

R

T

A

D

I

I

A

T

G

V

D

A

S

D

V

V

S

-

G

-

L

-

G

-

T

T

G

G

M

V

S

H

T

S

K

K

L

M

Q

E

A

A

binding adenosine-5'-diphosphate: G9 (= G12), G10 (≠ T13), S11 (= S14), D172 (= D170), I173 (≠ Q171), Y177 (= Y175), D180 (= D178)
binding (phenylmethyl) dihydrogen phosphate: A47 (= A41), G51 (vs. gap), H52 (= H43), I148 (≠ V146), S150 (≠ D148)

7n9dA I74a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
27% identity, 58% coverage: 7:220/367 of query aligns to 3:217/253 of 7n9dA

query
sites
7n9dA

L

I

V

L

V

I

K

K

L

L

G
|
G

T
x
G

S
|
S

V

V

L

I

T

T

G

D

G
x
K

S

S

R

E

-

Y

-

H

R

K

L

F

N

N

R

K

A

E

H

T

I

V

V

S

E

R

L

L

-

A

-

D

-

E

V

I

R

R

Q

R

C

S

A

G

Q

Q

-

D

-

V

-

M

-

V

L

V

H

H

A
x
G

A
|
A

G

G

-

S

-

F

-
x
G

H
|
H

R

-

I

-

V

V

I

I

V

A

T

K

S

K

G

Y

A

A

I

I

A

Q

A

D

G

G

R

-

E

-

H

H

L

V

G

D

Y

D

P

G

E

Q

L

I

P

P

A

A

T

-

I

-

A

A

S

A

K

R

Q
x
A

L

M

L

C

A

D

A
x
T

V

R

G

E

Q

L

S

S

R

S

L

M

I

V

-

V

-

E

Q

E

L

L

W

L

E

A

Q

Q

L

G

F

I

S

P

I

A

Y

V

G

S

I

V

H

A

V

P

G

G

Q

S

M

C

L

F

L

V

T

-

R

-

A

-

D

-

M

M

E

E

D

D

R

G

E

K

R

L

F

I

L

V

N

D

A

N

R

E

D

E

T

P

L

I

R

R

A

R

L

L

L

A

D

D

N

L

N

G

I

I

V

M

P

P

V

V

I

M

N

F

E

-

N
x
G

D
|
D

A
x
V

V

V

A

P

T

D

A

R

E

K

-

K

-

G

I

F

K

A

V
x
I

G

V

D
x
S

N
x
G

D

D

N

Q

L

C

S

M

A

E

L

V

A

L

A

C

I

R

L

M

A

F

G

D

A

P

D

E

K

K

L

V

L

F

L

V

T
x
S

D
|
D

Q
x
I

K

D

G

G

L

L

Y
|
Y

T

T

A
|
A

D
|
D

P

P

R

K

S

T

N

D

P

K

Q

K

A

A

E

R

L

L

I

I

K

G

D

E

V

V

Y

T

G

R

I

K

D

K

D

L

A

A

L

L

R

T

A
x
D

I

I

A

T

G

V

D

A

S

D

V

V

S

-

G

-

L

-

G

-

T
|
T

G

G

G
|
G

M
x
V

S

H

T

S

K
|
K

L

M

Q

E

A

A

binding (2E)-3-phenylbut-2-en-1-yl dihydrogen phosphate: G45 (≠ A40), A46 (= A41), G50 (vs. gap), H51 (= H43), A75 (≠ Q73), T79 (≠ A77), G135 (≠ N136), D136 (= D137), V137 (≠ A138), I147 (≠ V146), S149 (≠ D148), G150 (≠ N149), T208 (= T211)
binding adenosine-5'-diphosphate: G8 (= G12), G9 (≠ T13), S10 (= S14), K15 (≠ G19), S170 (≠ T169), D171 (= D170), I172 (≠ Q171), Y176 (= Y175), A178 (= A177), D179 (= D178), D201 (≠ A200), G210 (= G213), V211 (≠ M214), K214 (= K217)

Q8U122 Uridylate kinase; UK; Uridine monophosphate kinase; UMP kinase; UMPK; EC 2.7.4.22 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
35% identity, 23% coverage: 149:233/367 of query aligns to 120:201/225 of Q8U122

query
sites
Q8U122

N
x
T

D
|
D

N

A

L

V

S

A

A

A

L

L

A

L

A

A

I

E

L

F

A

L

G

K

A

A

D

D

K

L

L

L

L

V

L

I

T

T

D

N

Q

V

K

D

G

G

L

V

Y

Y

T

T

A

A

D

D

P

P

R

K

S

K

N

D

P

P

Q

T

A

A

E

K

L

K

I

I

K

K

D

K

V

M

Y

K

G

-

I

-

D

P

D

E

A

E

L

L

R

L

A

E

I

I

A

V

G

G

D

K

S

G

V

I

S

E

G

K

L

A

G
|
G

T

S

G

S

G
x
S

M

V

S

I

T

D

K

P

L

L

Q

-

A

A

D

K

V

I

A

I

C

A

R

R

A

S

G

G

I

I

D

K

T

T

I

I

I

V

T120 (≠ N149) binding
D121 (= D150) binding ; binding
G179 (= G210) binding
S182 (≠ G213) binding

Sites not aligning to the query:

6 binding
44 binding
66 binding
114:120 binding

2bmuB Ump kinase from pyrococcus furiosus complexed with its substrate ump and its substrate analog amppnp (see paper)
35% identity, 23% coverage: 149:233/367 of query aligns to 121:202/226 of 2bmuB

query
sites
2bmuB

N
x
T

D
|
D

N

A

L

V

S

A

A

A

L

L

A

L

A

A

I

E

L

F

A

L

G

K

A

A

D

D

K

L

L

L

L

V

L

I

T
|
T

D
x
N

Q

V

K

D

G

G

L
x
V

Y
|
Y

T

T

A
|
A

D
|
D

P
|
P

R

K

S

K

N

D

P

P

Q

T

A

A

E

K

L

K

I

I

K

K

D

K

V

M

Y

K

G

-

I

-

D

P

D

E

A

E

L

L

R

L

A

E

I

I

A

V

G

G

D

K

S

G

V

I

S

E

G

K

L
x
A

G

G

T

S

G
x
S

M
x
V

S

I

T

D

K

P

L

L

Q

-

A

A

D

K

V

I

A

I

C

A

R

R

A

S

G

G

I

I

D

K

T

T

I

I

I

V

binding phosphoaminophosphonic acid-adenylate ester: T141 (= T169), N142 (≠ D170), V146 (≠ L174), Y147 (= Y175), A149 (= A177), D150 (= D178), P151 (= P179), S182 (≠ G212), S183 (≠ G213), V184 (≠ M214)
binding magnesium ion: D122 (= D150), D122 (= D150), S183 (≠ G213), V184 (≠ M214)
binding uridine-5'-monophosphate: T121 (≠ N149), A179 (≠ L209)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 9, 10, 44, 45, 46
binding magnesium ion: 7
binding uridine-5'-monophosphate: 44, 45, 67, 70, 71, 114, 115, 116, 119, 120

7lntA Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to benzyl monophosphate and atp (see paper)
26% identity, 51% coverage: 4:191/367 of query aligns to 1:193/238 of 7lntA

query
sites
7lntA

S

S

Q

H

T

M

L

I

V

L

V

I

K

K

L

L

G
|
G

T

G

S
|
S

V

V

L

I

T

T

G

D

G
x
K

S

S

R

E

-

Y

-

H

R

K

L

F

N

N

R

K

A

E

H

T

I

V

V

S

E

R

L

L

-

A

-

D

-

E

V

I

R

R

Q

R

C

S

A

G

Q

Q

-

D

-

V

-

M

-

V

L

V

H

H

A
x
G

A
|
A

G

G

-

S

-

F

-
x
G

H
|
H

R

-

I

-

V

V

I

I

V

A

T

K

S

K

G

Y

A

A

I

I

A

Q

A

D

G

G

R

-

E

-

H

H

L

V

G

D

Y

D

P

G

E

Q

L

I

P

P

A

A

T

-

I

-

A

A

S

A

K

R

Q

I

L

M

L

C

A

D

A

T

V

R

G

E

Q

L

S

S

R

S

L

M

I

V

-

V

-

E

Q

E

L

L

W

L

E

A

Q

Q

L

G

F

I

S

P

I

A

Y

V

G

S

I

V

H

A

V

P

G

G

Q

S

M

C

L

F

L

V

T

-

R

-

A

-

D

-

M

M

E

E

D

D

R

G

E

K

R

L

F

I

L

V

N

D

A

N

R

E

D

E

T

P

L

I

R

R

A

R

L

L

L

A

D

D

N

L

N

G

I

I

V

M

P

P

V

V

I

M

N

F

E

-

N

G

D

D

A

V

V

V

A

P

T

D

A

R

E

K

-

K

-

G

I

F

K

A

V
x
I

G

V

D
x
S

N
x
G

D

D

N

Q

L

C

S

M

A

E

L

V

A

L

A

C

I

R

L

M

A

F

G

D

A

P

D

E

K

K

L

V

L

F

L

V

T
x
S

D
|
D

Q
x
I

K

D

G

G

L

L

Y
|
Y

T

T

A
|
A

D
|
D

P
|
P

R
x
K

S

T

N

D

P

K

Q

K

A

A

E

R

L

L

I

I

K

G

D

E

V

V

binding adenosine-5'-diphosphate: G9 (= G12), S11 (= S14), K16 (≠ G19), S171 (≠ T169), D172 (= D170), I173 (≠ Q171), Y177 (= Y175), A179 (= A177), D180 (= D178), P181 (= P179), K182 (≠ R180)
binding (phenylmethyl) dihydrogen phosphate: G46 (≠ A40), A47 (= A41), G51 (vs. gap), H52 (= H43), I148 (≠ V146), S150 (≠ D148), G151 (≠ N149)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 196

7lnuA Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to isopentenyl monophosphate and atp (see paper)
26% identity, 51% coverage: 4:191/367 of query aligns to 1:193/239 of 7lnuA

query
sites
7lnuA

S

S

Q

H

T

M

L

I

V

L

V

I

K
|
K

L

L

G
|
G

T
x
G

S
|
S

V

V

L

I

T

T

G

D

G
x
K

S

S

R

E

-

Y

-

H

R

K

L

F

N

N

R

K

A

E

H

T

I

V

V

S

E

R

L

L

-

A

-

D

-

E

V

I

R

R

Q

R

C

S

A

G

Q

Q

-

D

-

V

-

M

-

V

L

V

H

H

A
x
G

A
|
A

G

G

-

S

-

F

-
x
G

H
|
H

R

-

I

-

V

V

I

I

V

A

T

K

S

K

G

Y

A

A

I

I

A

Q

A

D

G

G

R

-

E

-

H

H

L

V

G

D

Y

D

P

G

E

Q

L

I

P

P

A

A

T

-

I

-

A

A

S

A

K

R

Q

I

L

M

L

C

A

D

A

T

V

R

G

E

Q

L

S

S

R

S

L

M

I

V

-

V

-

E

Q

E

L

L

W

L

E

A

Q

Q

L

G

F

I

S

P

I

A

Y

V

G

S

I

V

H

A

V

P

G

G

Q

S

M

C

L

F

L

V

T

-

R

-

A

-

D

-

M

M

E

E

D

D

R

G

E

K

R

L

F

I

L

V

N

D

A

N

R

E

D

E

T

P

L

I

R

R

A

R

L

L

L

A

D

D

N

L

N

G

I

I

V

M

P

P

V

V

I

M

N

F

E

-

N
x
G

D

D

A

V

V

V

A

P

T

D

A

R

E

K

-

K

-

G

I

F

K

A

V
x
I

G

V

D
x
S

N
x
G

D

D

N

Q

L

C

S

M

A

E

L

V

A

L

A

C

I

R

L

M

A

F

G

D

A

P

D

E

K

K

L

V

L

F

L

V

T

S

D
|
D

Q
x
I

K

D

G

G

L
|
L

Y

Y

T

T

A
|
A

D
|
D

P

P

R
x
K

S

T

N

D

P

K

Q

K

A

A

E

R

L

L

I

I

K

G

D

E

V

V

binding adenosine-5'-diphosphate: G9 (= G12), G10 (≠ T13), S11 (= S14), K16 (≠ G19), D172 (= D170), I173 (≠ Q171), L176 (= L174), D180 (= D178), K182 (≠ R180)
binding adenosine-5'-triphosphate: K7 (= K10), G9 (= G12), G10 (≠ T13), S11 (= S14), K16 (≠ G19), D172 (= D170), L176 (= L174), A179 (= A177), D180 (= D178), K182 (≠ R180)
binding Isopentenyl phosphate: G46 (≠ A40), A47 (= A41), G51 (vs. gap), H52 (= H43), G136 (≠ N136), I148 (≠ V146), S150 (≠ D148), G151 (≠ N149)
binding 3-methylbut-3-enyl trihydrogen diphosphate: A47 (= A41), G51 (vs. gap), H52 (= H43), G136 (≠ N136), I148 (≠ V146), G151 (≠ N149)

7lnxB I146a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
26% identity, 51% coverage: 4:191/367 of query aligns to 4:196/247 of 7lnxB

query
sites
7lnxB

S

S

Q

H

T

M

L

I

V

L

V

I

K
|
K

L

L

G

G

T

G

S
|
S

V

V

L

I

T

T

G

D

G

K

S

S

R

E

-

Y

-

H

R

K

L

F

N

N

R

K

A

E

H

T

I

V

V

S

E

R

L

L

-

A

-

D

-

E

V

I

R

R

Q

R

C

S

A

G

Q

Q

-

D

-

V

-

M

-

V

L

V

H

H

A

G

A
|
A

G

G

-

S

-

F

-
x
G

H
|
H

R

-

I

-

V

V

I

I

V
x
A

T

K

S

K

G

Y

A

A

I

I

A

Q

A

D

G

G

R

-

E

-

H

H

L

V

G

D

Y

D

P

G

E

Q

L

I

P

P

A

A

T

-

I

-

A

A

S

A

K

R

Q

I

L

M

L

C

A

D

A

T

V

R

G

E

Q

L

S

S

R

S

L

M

I

V

-

V

-

E

Q

E

L

L

W

L

E

A

Q

Q

L

G

F

I

S

P

I

A

Y

V

G

S

I

V

H

A

V

P

G

G

Q

S

M

C

L

F

L

V

T

-

R

-

A

-

D

-

M

M

E

E

D

D

R

G

E

K

R

L

F

I

L

V

N

D

A

N

R

E

D

E

T

P

L

I

R

R

A

R

L

L

L

A

D

D

N

L

N

G

I

I

V

M

P

P

V

V

I

M

N

F

E

-

N

G

D
|
D

A
x
V

V

V

A

P

T

D

A

R

E

K

-

K

-

G

I

F

K

A

V
x
A

G

V

D

S

N
x
G

D

D

N

Q

L

C

S

M

A

E

L

V

A

L

A

C

I

R

L

M

A

F

G

D

A

P

D

E

K

K

L

V

L

F

L

V

T
x
S

D
|
D

Q
x
I

K

D

G

G

L

L

Y
|
Y

T

T

A
|
A

D
|
D

P
|
P

R
x
K

S

T

N

D

P

K

Q

K

A

A

E

R

L

L

I

I

K

G

D

E

V

V

binding adenosine-5'-diphosphate: K10 (= K10), S14 (= S14), S174 (≠ T169), D175 (= D170), I176 (≠ Q171), Y180 (= Y175), A182 (= A177), D183 (= D178), P184 (= P179), K185 (≠ R180)
binding (2E)-3-methylhept-2-en-1-yl dihydrogen phosphate: A50 (= A41), G54 (vs. gap), H55 (= H43), A58 (≠ V48), D140 (= D137), V141 (≠ A138), A151 (≠ V146), G154 (≠ N149)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 204

7lnwA I146a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
27% identity, 54% coverage: 5:201/367 of query aligns to 1:204/256 of 7lnwA

query
sites
7lnwA

Q

H

T

M

L

I

V

L

V

I

K
|
K

L

L

G

G

T

G

S
|
S

V

V

L

I

T

T

G

D

G
x
K

S

S

R

E

-

Y

-

H

R

K

L

F

N

N

R

K

A

E

H

T

I

V

V

S

E

R

L

L

-

A

-

D

-

E

V

I

R

R

Q

R

C

S

A

G

Q

Q

-

D

-

V

-

M

-

V

L

V

H

H

A
x
G

A
|
A

G

G

-

S

-

F

-
x
G

H
|
H

R

-

I

-

V

V

I

I

V

A

T

K

S

K

G

Y

A

A

I

I

A

Q

A

D

G

G

R

-

E

-

H

H

L

V

G

D

Y

D

P

G

E

Q

L

I

P

P

A

A

T

-

I

-

A

A

S

A

K

R

Q

I

L

M

L

C

A

D

A

T

V

R

G

E

Q

L

S

S

R

S

L

M

I

V

-

V

-

E

Q

E

L

L

W

L

E

A

Q

Q

L

G

F

I

S

P

I

A

Y

V

G

S

I

V

H

A

V

P

G

G

Q

S

M

C

L

F

L

V

T

-

R

-

A

-

D

-

M

M

E

E

D

D

R

G

E

K

R

L

F

I

L

V

N

D

A

N

R

E

D

E

T

P

L

I

R

R

A

R

L

L

L

A

D

D

N

L

N

G

I

I

V

M

P

P

V

V

I

M

N

F

E

-

N
x
G

D
|
D

A

V

V

V

A

P

T

D

A

R

E

K

-

K

-

G

I

F

K

A

V

A

G
x
V

D
x
S

N

G

D

D

N

Q

L

C

S

M

A

E

L

V

A

L

A

C

I

R

L

M

A

F

G

D

A

P

D

E

K

K

L

V

L

F

L

V

T

S

D
|
D

Q
x
I

K

D

G

G

L

L

Y
|
Y

T

T

A

A

D
|
D

P
|
P

R
x
K

S

T

N

D

P

K

Q

K

A

A

E

R

L

L

I

I

K

G

D

E

V

V

-

T

-

R

Y

K

G

K

I

L

D

D

D

E

A

A

L

L

R

T

A

D

I

I

binding adenosine-5'-diphosphate: K6 (= K10), S10 (= S14), K15 (≠ G19), D171 (= D170), I172 (≠ Q171), Y176 (= Y175), D179 (= D178), P180 (= P179), K181 (≠ R180)
binding [(~{Z})-5-[(azanylidene-$l^{4}-azanylidene)amino]-3-methyl-pent-2-enyl] dihydrogen phosphate: G45 (≠ A40), A46 (= A41), G50 (vs. gap), H51 (= H43), G135 (≠ N136), D136 (= D137), V148 (≠ G147), S149 (≠ D148)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 210, 213

2ji5A Structure of ump kinase from pyrococcus furiosus complexed with utp
35% identity, 23% coverage: 149:233/367 of query aligns to 122:195/219 of 2ji5A

query
sites
2ji5A

N
x
T

D

D

N

A

L

V

S

A

A

A

L

L

A

L

A

A

I

E

L

F

A

L

G

K

A

A

D

D

K

L

L

L

L

V

L

I

T

T

D

N

Q

V

K

D

G

G

L

V

Y

Y

T

T

A

A

D

D

P

P

R

K

S

K

N

D

P

P

Q

T

A

A

E

K

L

K

I

I

K

K

D

K

V

M

Y

K

G

-

I

-

D

P

D

E

A

E

L

L

R

L

A

E

I

I

A

V

G

G

D

K

S

S

V

V

S

-

G

-

L

-

G

-

T

-

G

-

M

-

S

-

T

I

K

D

L

P

Q

L

A

A

D

K

V

I

A

I

C

A

R

R

A

T

G

G

I

I

D

K

T

T

I

I

I

V

binding uridine 5'-triphosphate: T122 (≠ N149)

Sites not aligning to the query:

binding uridine 5'-triphosphate: 68, 71, 72, 115, 116, 117, 120, 121

Query Sequence

>14381 FitnessBrowser__Keio:14381
MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHL
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDT
LRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPR
SNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPG
VIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGI
KSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVH
RDDMITR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory