SitesBLAST
Comparing 14449 FitnessBrowser__Keio:14449 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
37% identity, 97% coverage: 2:538/556 of query aligns to 4:457/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G9), G13 (= G11), S14 (= S12), A15 (= A13), E35 (= E33), A36 (= A34), W47 (= W62), P65 (≠ G80), G67 (= G82), V180 (≠ T222), A214 (= A258), G215 (= G259), A218 (= A262), T270 (= T335), Y391 (≠ F472), A424 (= A505), I435 (≠ L516), N436 (= N517)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
36% identity, 96% coverage: 2:532/556 of query aligns to 13:527/530 of 3ljpA
- active site: I333 (≠ A345), P377 (≠ V386), N378 (≠ G387), A464 (= A471), H466 (= H473), V509 (≠ G514), N510 (= N515)
- binding dihydroflavine-adenine dinucleotide: G22 (= G11), S23 (= S12), E44 (= E33), A45 (= A34), W71 (= W62), R89 (= R81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ G93), I103 (≠ C95), A232 (≠ T222), T269 (≠ A258), D273 (≠ A262), Y465 (≠ F472), H466 (= H473), D499 (= D504), A500 (= A505), N510 (= N515), P511 (≠ L516), N512 (= N517), V515 (≠ T520)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
36% identity, 96% coverage: 2:536/556 of query aligns to 13:531/532 of 4mjwA
- active site: I333 (≠ A345), P377 (≠ V386), N378 (≠ G387), V464 (≠ A471), H466 (= H473), V509 (≠ G514), N510 (= N515)
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), S23 (= S12), E44 (= E33), A45 (= A34), W71 (= W62), R89 (= R81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ G93), I103 (≠ C95), R231 (≠ M221), A232 (≠ T222), T269 (≠ A258), G270 (= G259), D273 (≠ A262), Y465 (≠ F472), H466 (= H473), A500 (= A505), N510 (= N515), P511 (≠ L516), N512 (= N517), V515 (≠ T520)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
34% identity, 97% coverage: 2:540/556 of query aligns to 23:578/578 of 5nccA
- active site: R347 (≠ P324), L420 (≠ V386), I421 (≠ G387), S507 (≠ A471), A509 (≠ H473), G552 (= G514), Q553 (≠ N515)
- binding flavin-adenine dinucleotide: G30 (= G9), G32 (= G11), T33 (≠ S12), A34 (= A13), L53 (= L32), E54 (= E33), A55 (= A34), F74 (≠ L55), W80 (= W62), A98 (≠ G80), G100 (= G82), G105 (= G87), S106 (= S88), N110 (= N92), A111 (≠ G93), T112 (≠ M94), L113 (≠ C95), V238 (≠ T222), A278 (= A258), H282 (≠ A262), L286 (≠ I266), N508 (≠ F472), Q553 (≠ N515), T554 (≠ L516), G555 (≠ N517), V558 (≠ T520)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
36% identity, 96% coverage: 2:532/556 of query aligns to 13:527/527 of 2jbvA
- active site: I333 (≠ A345), P377 (≠ V386), N378 (≠ G387), V464 (≠ A471), H466 (= H473), V509 (≠ G514), N510 (= N515)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G11), S23 (= S12), E44 (= E33), A45 (= A34), W71 (= W62), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ G93), I103 (≠ C95), R231 (≠ M221), A232 (≠ T222), T269 (≠ A258), G270 (= G259), D273 (≠ A262), V464 (≠ A471), Y465 (≠ F472), H466 (= H473), D499 (= D504), A500 (= A505), N510 (= N515), P511 (≠ L516), N512 (= N517), V515 (≠ T520)
6yrvAAA structure of fap after illumination at 100k (see paper)
34% identity, 97% coverage: 2:542/556 of query aligns to 7:571/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H363), N499 (≠ F472)
- binding flavin-adenine dinucleotide: G14 (= G9), G16 (= G11), T17 (≠ S12), A18 (= A13), L37 (= L32), E38 (= E33), A39 (= A34), F58 (≠ L55), W64 (= W62), A82 (≠ G80), G89 (= G87), S90 (= S88), N94 (= N92), A95 (≠ G93), T96 (≠ M94), L97 (≠ C95), M191 (≠ V191), V222 (≠ T222), C264 (= C257), A265 (= A258), G266 (= G259), H269 (≠ A262), N499 (≠ F472), A534 (= A505), Q544 (≠ N515), T545 (≠ L516), G546 (≠ N517)
- binding heptadecane: V377 (≠ L365), G379 (vs. gap), M380 (≠ V367), G386 (= G373), T389 (≠ N375), Y390 (≠ A376), F393 (≠ E379), T408 (vs. gap), Q410 (vs. gap)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
34% identity, 96% coverage: 2:537/556 of query aligns to 83:642/654 of A0A248QE08
- TA 93:94 (≠ SA 12:13) binding
- E114 (= E33) binding
- L162 (≠ G84) binding
- S166 (= S88) binding
- NATL 170:173 (≠ NGMC 92:95) binding
- V298 (≠ T222) binding
- C432 (≠ F343) binding
- R451 (≠ H363) binding
- Y466 (≠ A376) binding
- Q486 (vs. gap) binding
- G622 (≠ N517) binding
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
31% identity, 94% coverage: 4:528/556 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (≠ V386), G361 (= G387), H444 (≠ A471), H446 (= H473), G487 (= G514), P488 (≠ N515)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (= A13), E32 (= E33), A33 (= A34), W58 (= W62), R77 (= R81), G78 (= G82), G83 (= G87), S84 (= S88), L87 (≠ I91), H88 (≠ N92), A89 (≠ G93), M90 (= M94), G91 (≠ C95), V218 (≠ T222), A251 (= A258), G252 (= G259), E255 (≠ A262), H445 (≠ F472), A478 (= A505), P488 (≠ N515), I489 (≠ L516), H490 (≠ N517)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G93), S314 (≠ F343), H444 (≠ A471), H446 (= H473)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
31% identity, 94% coverage: 4:528/556 of query aligns to 3:501/509 of 3t37A
- active site: F360 (≠ V386), G361 (= G387), H444 (≠ A471), H446 (= H473), G487 (= G514), P488 (≠ N515)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (= A13), E32 (= E33), A33 (= A34), W58 (= W62), R77 (= R81), G78 (= G82), R79 (≠ K83), G83 (= G87), S84 (= S88), H88 (≠ N92), A89 (≠ G93), G91 (≠ C95), R217 (≠ M221), V218 (≠ T222), A251 (= A258), E255 (≠ A262), H445 (≠ F472), A478 (= A505), P488 (≠ N515), I489 (≠ L516), H490 (≠ N517)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
31% identity, 96% coverage: 3:534/556 of query aligns to 6:530/531 of E4QP00
- V101 (≠ I91) mutation to H: Abolishes activity.
- M103 (≠ G93) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (= V386) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ S388) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ A471) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ F472) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H473) mutation to A: Abolishes activity.
- N511 (= N515) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
31% identity, 95% coverage: 3:531/556 of query aligns to 2:523/525 of 4udqA
- active site: L331 (≠ A345), F364 (≠ G387), W365 (≠ S388), V461 (≠ A471), H463 (= H473), A506 (≠ G514), N507 (= N515)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), T11 (≠ S12), A12 (= A13), E32 (= E33), A33 (= A34), W64 (= W62), G88 (= G82), G93 (= G87), G94 (≠ S88), N98 (= N92), M99 (≠ G93), V101 (≠ C95), V229 (≠ T222), T261 (≠ C257), A262 (= A258), W462 (≠ F472), H463 (= H473), A497 (= A505), N507 (= N515), T508 (≠ L516), N509 (= N517), T512 (= T520)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
30% identity, 95% coverage: 3:532/556 of query aligns to 5:564/570 of 4yntA
- active site: V342 (vs. gap), F413 (≠ G381), W414 (≠ F382), N502 (≠ A471), H504 (= H473), G546 (= G514), H547 (≠ N515)
- binding dihydroflavine-adenine dinucleotide: G13 (= G11), T14 (≠ S12), S15 (≠ A13), E35 (= E33), A36 (= A34), F56 (= F53), W62 (= W62), R80 (≠ G80), G82 (= G82), G87 (= G87), T88 (≠ S88), N92 (= N92), G93 (= G93), M94 (= M94), A95 (≠ C95), A234 (≠ T222), A274 (= A258), R278 (≠ A262), F503 (= F472), A537 (= A505), H547 (≠ N515), L548 (= L516), V549 (≠ N517), L552 (≠ T520)
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
30% identity, 95% coverage: 3:532/556 of query aligns to 4:563/569 of 4ynuA
- active site: V341 (vs. gap), F412 (≠ G381), W413 (≠ F382), N501 (≠ A471), H503 (= H473), G545 (= G514), H546 (≠ N515)
- binding flavin-adenine dinucleotide: G12 (= G11), T13 (≠ S12), S14 (≠ A13), E34 (= E33), A35 (= A34), Y51 (≠ A49), F55 (= F53), W61 (= W62), R79 (≠ G80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (= G93), T232 (≠ M221), A233 (≠ T222), A273 (= A258), G274 (= G259), R277 (≠ A262), F502 (= F472), A536 (= A505), H546 (≠ N515), L547 (= L516), V548 (≠ N517), L551 (≠ T520)
- binding D-glucono-1,5-lactone: Y51 (≠ A49), E411 (≠ H380), A496 (≠ N466), N497 (≠ H467), R499 (≠ E469), R499 (≠ E469), N501 (≠ A471), H503 (= H473), H546 (≠ N515)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
30% identity, 95% coverage: 3:532/556 of query aligns to 4:563/566 of 7vzsA
- binding D-glucal: Y6 (= Y5), L22 (= L21), N25 (≠ D24), Y51 (≠ A49), I349 (= I326), Q356 (≠ G333), E411 (≠ H380), E444 (= E418), W445 (≠ Q419), K448 (≠ Q422), R499 (≠ E469), N501 (≠ A471), H546 (≠ N515), K563 (≠ R532)
- binding flavin-adenine dinucleotide: G10 (= G9), G12 (= G11), T13 (≠ S12), S14 (≠ A13), E34 (= E33), A35 (= A34), Y51 (≠ A49), F55 (= F53), W61 (= W62), R79 (≠ G80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (= G93), M93 (= M94), A94 (≠ C95), T232 (≠ M221), A233 (≠ T222), A273 (= A258), G274 (= G259), R277 (≠ A262), F502 (= F472), A536 (= A505), H546 (≠ N515), L547 (= L516), V548 (≠ N517), L551 (≠ T520)
Sites not aligning to the query:
8bxlB Patulin synthase from penicillium expansum
32% identity, 96% coverage: 3:533/556 of query aligns to 14:588/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), T23 (≠ S12), A24 (= A13), E44 (= E33), A45 (= A34), W80 (= W62), G100 (= G82), G105 (= G87), S106 (= S88), R109 (≠ I91), N110 (= N92), Y111 (≠ G93), A113 (≠ C95), L253 (≠ M221), A254 (≠ T222), A288 (= A258), Q292 (≠ A262), F525 (= F472), D559 (= D504), A560 (= A505), H570 (≠ N515), P571 (≠ L516), Q572 (≠ N517), L575 (≠ T520)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
28% identity, 96% coverage: 3:535/556 of query aligns to 2:565/565 of 5oc1A
- active site: V339 (≠ P316), N413 (≠ G387), A414 (≠ S388), I499 (≠ A471), H501 (= H473), A544 (≠ G514), H545 (≠ N515)
- binding 4-methoxybenzoic acid: Y91 (≠ G93), I356 (vs. gap), I390 (≠ A356), F396 (≠ Y362), T412 (≠ V386), I499 (≠ A471), H501 (= H473), H545 (≠ N515)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), N11 (≠ S12), A12 (= A13), E32 (= E33), A33 (= A34), W60 (= W62), P78 (≠ G80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (≠ G93), V93 (≠ C95), V230 (≠ T222), S270 (≠ C257), A271 (= A258), G272 (= G259), F500 (= F472), H545 (≠ N515), T546 (≠ L516), Q547 (≠ N517), I550 (≠ T520)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
28% identity, 96% coverage: 3:535/556 of query aligns to 2:565/565 of 3fimB
- active site: V339 (≠ P316), N413 (≠ G387), A414 (≠ S388), I499 (≠ A471), H501 (= H473), A544 (≠ G514), H545 (≠ N515)
- binding flavin-adenine dinucleotide: G8 (= G9), N11 (≠ S12), A12 (= A13), E32 (= E33), A33 (= A34), W60 (= W62), P78 (≠ G80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (≠ G93), V93 (≠ C95), V230 (≠ T222), S270 (≠ C257), A271 (= A258), F500 (= F472), H501 (= H473), H545 (≠ N515), T546 (≠ L516), Q547 (≠ N517), I550 (≠ T520)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
28% identity, 92% coverage: 1:510/556 of query aligns to 14:551/577 of 4h7uA
- active site: A343 (≠ P316), V426 (≠ G387), Y510 (≠ A471), H512 (= H473)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G9), G24 (= G11), T25 (≠ S12), A26 (= A13), E46 (= E33), A47 (= A34), W74 (= W62), G99 (= G87), C100 (≠ S88), H103 (≠ I91), N104 (= N92), G105 (= G93), V107 (≠ C95), L242 (≠ M221), V243 (≠ T222), G282 (≠ A258), G283 (= G259), A286 (= A262), H512 (= H473), A546 (= A505)
Sites not aligning to the query:
- active site: 555, 556
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): 556, 557, 558, 561
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
28% identity, 92% coverage: 1:510/556 of query aligns to 39:576/602 of Q3L245
- N100 (≠ A63) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I91) modified: Tele-8alpha-FAD histidine
- N344 (= N295) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H473) active site, Proton acceptor
Sites not aligning to the query:
- 1:25 signal peptide
- 581 active site
6h3oF Alcohol oxidase from phanerochaete chrysosporium mutant f101s (see paper)
27% identity, 97% coverage: 2:541/556 of query aligns to 5:629/650 of 6h3oF
- binding flavin-adenine dinucleotide: P15 (≠ S12), E37 (= E33), G38 (= G35), N90 (≠ K83), I91 (≠ G84), G94 (= G87), G95 (≠ S88), N99 (= N92), S100 (≠ G93), Q101 (≠ M94), M102 (≠ C95), R227 (≠ M221), V228 (≠ T222), S271 (≠ A258), I279 (= I266), W559 (≠ F472), L593 (≠ A505), N603 (= N515), T604 (≠ L516), Y605 (≠ N517), A608 (≠ T520)
Query Sequence
>14449 FitnessBrowser__Keio:14449
MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFRTQMPAALAFPLQGKRY
NWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNALDLDNWAQEPGLENWSYLD
CLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEAMIEAGVQAGYPRTDDLNGYQ
QEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRTHAMTDHIIFDGKRAVGVEWLEG
DSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAELLAEFDIPLVHELPGVGENLQDHL
EMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGGTGVGASNHFEAGGFIRSREEFAWPNI
QYHFLPVAINYNGSNAVKEHGFQCHVGSMRSPSRGHVRIKSRDPHQHPAILFNYMSHEQD
WQEFRDAIRITREIMHQPALDQYRGREISPGVECQTDEQLDEFVRNHAETAFHPCGTCKM
GYDEMSVVDGEGRVHGLEGLRVVDASIMPQIITGNLNATTIMIGEKIADMIRGQEALPRS
TAGYFVANGMPVRAKK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory