SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 14480 FitnessBrowser__Keio:14480 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
100% identity, 100% coverage: 1:203/203 of query aligns to 1:203/203 of P07464

query
sites
P07464

M
|
M

N

N

M

M

P

P

M

M

T

T

E

E

R

R

I

I

R

R

A

A

G

G

K

K

L

L

F

F

T

T

D
|
D

M

M

C

C

E

E

G

G

L

L

P

P

E

E

K

K

R

R

L

L

R

R

G

G

K

K

T

T

L

L

M

M

Y

Y

E

E

F

F

N

N

H

H

S

S

H

H

P

P

S

S

E

E

V

V

E

E

K

K

R

R

E

E

S

S

L

L

I

I

K

K

E

E

M

M

F

F

A

A

T

T

V

V

G

G

E

E

N

N

A

A

W

W

V

V

E

E

P

P

V

V

Y

Y

F

F

S
|
S

Y

Y

G

G

S

S

N

N

I

I

H

H

I

I

G

G

R

R

N

N

F

F

Y

Y

A

A

N
|
N

F

F

N

N

L

L

T

T

I

I

V

V

D

D

D
|
D

Y

Y

T

T

V

V

T

T

I

I

G

G

D

D

N

N

V

V

L

L

I

I

A

A

P

P

N

N

V

V

T

T

L

L

S

S

V

V

T

T

G

G

H
|
H

P

P

V

V

H

H

E

E

L

L

R

R

K

K

N

N

G

G

E

E

M

M

Y

Y

S

S

F

F

P

P

I

I

T

T

I

I

G

G

N

N

V

V

W

W

I

I

G

G

S
|
S

H

H

V

V

I

I

N

N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

N

N

S

S

V

V

I

I

G

G

A
|
A

G

G

S

S

I

I

V

V

T
|
T

K
|
K

D

D

I

I

P

P

N

N

V

V

A

A

G

G

V

V

P

P

C

C

R
|
R

V

V

I

I

R
|
R

E

E

I

I

N

N

D

D

R

R

D

D

K

K

H

H

Y

Y

F

F

K

K

D

D

Y

Y

K

K

V

V

E

E

S

S

V

V

M1 (= M1) modified: Initiator methionine, Removed; Partial
D17 (= D17) binding
S71 (= S71) binding
N85 (= N85) binding in other chain; binding in other chain
D93 (= D93) binding
H115 (= H115) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S142) binding in other chain
A160 (= A160) binding in other chain
TK 165:166 (= TK 165:166) binding
R180 (= R180) binding
R183 (= R183) binding in other chain

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
100% identity, 99% coverage: 2:202/203 of query aligns to 1:201/201 of 1krvA

query
sites
1krvA

N

N

M

M

P

P

M

M

T

T

E

E

R

R

I

I

R

R

A

A

G

G

K

K

L

L

F

F

T

T

D
|
D

M

M

C

C

E

E

G

G

L

L

P

P

E

E

K

K

R
|
R

L

L

R

R

G

G

K

K

T

T

L

L

M

M

Y

Y

E

E

F

F

N

N

H

H

S

S

H

H

P

P

S

S

E

E

V

V

E

E

K

K

R

R

E

E

S

S

L

L

I

I

K

K

E

E

M

M

F

F

A

A

T

T

V

V

G

G

E

E

N

N

A

A

W

W

V

V

E

E

P

P

V

V

Y

Y

F

F

S
|
S

Y

Y

G

G

S

S

N

N

I

I

H

H

I

I

G

G

R

R

N

N

F

F

Y
|
Y

A

A

N
|
N

F

F

N

N

L

L

T

T

I

I

V
|
V

D

D

D
|
D

Y

Y

T

T

V

V

T

T

I

I

G

G

D

D

N

N

V

V

L

L

I

I

A
|
A

P

P

N

N

V

V

T

T

L

L

S
|
S

V

V

T
|
T

G

G

H

H

P

P

V

V

H

H

E

E

L

L

R

R

K

K

N

N

G

G

E

E

M
|
M

Y

Y

S

S

F

F

P

P

I

I

T

T

I

I

G

G

N

N

V

V

W
|
W

I

I

G
|
G

S
|
S

H

H

V

V

I

I

N
|
N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

N

N

S

S

V

V

I

I

G

G

A
|
A

G

G

S

S

I

I

V

V

T

T

K

K

D

D

I

I

P

P

N

N

V

V

A

A

G

G

V

V

P
|
P

C

C

R
|
R

V

V

I

I

R
|
R

E

E

I

I

N

N

D

D

R

R

D

D

K

K

H

H

Y

Y

F

F

K

K

D

D

Y

Y

K

K

V

V

E

E

S

S

binding 4-nitrophenyl beta-D-galactopyranoside: D16 (= D17), R25 (= R26), S70 (= S71), Y82 (= Y83), N84 (= N85), V90 (= V91), D92 (= D93), M126 (= M127)
binding coenzyme a: A104 (= A105), S110 (= S111), T112 (= T113), W138 (= W139), G140 (= G141), S141 (= S142), N146 (= N147), A159 (= A160), P177 (= P178), R179 (= R180), R182 (= R183)

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
100% identity, 99% coverage: 2:202/203 of query aligns to 1:201/201 of 1kruA

query
sites
1kruA

N

N

M

M

P

P

M

M

T

T

E

E

R

R

I

I

R

R

A

A

G

G

K

K

L

L

F

F

T

T

D
|
D

M

M

C

C

E

E

G

G

L

L

P
|
P

E

E

K

K

R
|
R

L

L

R

R

G

G

K

K

T

T

L

L

M

M

Y

Y

E

E

F

F

N

N

H

H

S

S

H

H

P

P

S

S

E

E

V

V

E

E

K

K

R

R

E

E

S

S

L

L

I

I

K

K

E

E

M

M

F

F

A

A

T

T

V

V

G

G

E

E

N

N

A

A

W
|
W

V

V

E

E

P

P

V

V

Y

Y

F

F

S
|
S

Y

Y

G

G

S

S

N

N

I

I

H

H

I

I

G

G

R

R

N

N

F

F

Y
|
Y

A

A

N
|
N

F

F

N

N

L

L

T

T

I

I

V
|
V

D

D

D
|
D

Y

Y

T

T

V

V

T

T

I

I

G

G

D

D

N

N

V

V

L
|
L

I

I

A

A

P

P

N

N

V

V

T

T

L

L

S

S

V

V

T

T

G

G

H
|
H

P

P

V

V

H

H

E

E

L

L

R

R

K

K

N

N

G

G

E

E

M
|
M

Y

Y

S

S

F

F

P

P

I

I

T

T

I

I

G

G

N

N

V

V

W
|
W

I

I

G
|
G

S
|
S

H

H

V

V

I

I

N

N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

N

N

S

S

V

V

I

I

G
|
G

A
|
A

G

G

S

S

I

I

V

V

T

T

K

K

D

D

I

I

P

P

N

N

V

V

A

A

G

G

V

V

P
|
P

C

C

R

R

V

V

I

I

R
|
R

E

E

I

I

N

N

D

D

R

R

D

D

K

K

H

H

Y

Y

F

F

K

K

D

D

Y

Y

K

K

V

V

E

E

S

S

binding coenzyme a: H114 (= H115), W138 (= W139), G140 (= G141), S141 (= S142), G158 (= G159), A159 (= A160), P177 (= P178), R182 (= R183)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: D16 (= D17), P22 (= P23), R25 (= R26), W62 (= W63), S70 (= S71), Y82 (= Y83), Y82 (= Y83), N84 (= N85), V90 (= V91), D92 (= D93), L102 (= L103), H114 (= H115), M126 (= M127)

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
100% identity, 99% coverage: 2:201/203 of query aligns to 1:200/200 of 1krrA

query
sites
1krrA

N

N

M

M

P

P

M

M

T

T

E

E

R

R

I

I

R

R

A

A

G

G

K

K

L

L

F

F

T

T

D

D

M

M

C

C

E

E

G

G

L

L

P

P

E

E

K

K

R

R

L

L

R

R

G

G

K

K

T

T

L

L

M

M

Y

Y

E

E

F

F

N

N

H

H

S

S

H

H

P

P

S

S

E

E

V

V

E

E

K

K

R

R

E

E

S

S

L

L

I

I

K

K

E

E

M

M

F

F

A

A

T

T

V

V

G

G

E

E

N

N

A

A

W

W

V

V

E

E

P

P

V

V

Y

Y

F

F

S

S

Y

Y

G

G

S

S

N

N

I

I

H

H

I

I

G

G

R

R

N

N

F

F

Y

Y

A

A

N
|
N

F

F

N

N

L

L

T

T

I

I

V

V

D

D

Y

Y

T

T

V

V

T

T

I

I

G

G

D

D

N

N

V

V

L

L

I
|
I

A
|
A

P
|
P

N

N

V

V

T

T

L

L

S

S

V

V

T
|
T

G

G

H
|
H

P

P

V

V

H

H

E

E

L

L

R

R

K

K

N

N

G

G

E

E

M

M

Y

Y

S

S

F

F

P

P

I

I

T

T

I

I

G

G

N

N

V

V

W

W

I

I

G
|
G

S
|
S

H

H

V

V

I

I

N
|
N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

N

N

S

S

V

V

I

I

G
|
G

A
|
A

G

G

S

S

I

I

V

V

T

T

K

K

D

D

I

I

P

P

N

N

V

V

A

A

A
|
A

G

G

V

V

P
|
P

C

C

R

R

V

V

I

I

R
|
R

E

E

I

I

N

N

D

D

R

R

D

D

K

K

H

H

Y

Y

F

F

K

K

D

D

Y

Y

K

K

V

V

E

E

S

S

binding acetyl coenzyme *a: N84 (= N85), I103 (= I104), A104 (= A105), P105 (= P106), T112 (= T113), H114 (= H115), G140 (= G141), S141 (= S142), N146 (= N147), G158 (= G159), A159 (= A160), A174 (= A175), P177 (= P178), R182 (= R183)

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
47% identity, 91% coverage: 3:187/203 of query aligns to 2:186/186 of 4isxA

query
sites
4isxA

M

M

P

T

M

E

T

K

E

E

R

K

I

M

R

L

A

S

G

G

K

K

L

G

F

Y

T

Y

D

A

M

N

C

D

E

E

G

L

L

L

P

V

E

K

K

E

R

R

L

E

R

Y

G

C

K

K

T

K

L

L

M

T

Y

R

E

L

F

F

N

N

H

N

S

T

H

L

P

E

S

D

E

E

V

Y

E

E

K

K

R

R

E

E

S

D

L

I

I

L

K

R

E

Q

M

L

F

F

A

G

T

S

V

V

G

G

E

K

N

Q

A

I

W

N

V

V

E

E

P

Q

P

N

V

I

Y

R

F

C

S

D

Y

Y

G

G

S

Y

N

N

I

I

H

H

I

V

G

G

R

E

N

N

F

F

Y

F

A

A

N
|
N

F

Y

N

D

L

C

T

I

I

F

V

L

D

D

D

V

Y

C

T

K

V

I

T

E

I

I

G

G

D

D

N

N

V

V

L

M

I

L

A
|
A

P

P

N

N

V

V

T

Q

L

I

S

Y

V

T

T

A

G

Y

H

H

P

P

V

I

H

D

H

A

E

Q

L

L

R

R

K

N

N

S

G

G

E

I

M

E

Y

Y

S

G

F

S

P

P

I

V

T

K

I

I

G

G

N

D

N

N

V

V

W
|
W

I

I

G
|
G

S
x
G

H

G

V

V

V

I

I

I

N

T

P

P

G

G

V

I

T

T

I

I

G

G

D

D

N

N

S

V

V

V

I

I

G

G

A
|
A

G

G

S

S

I

V

V

V

T

T

K

K

D

D

I

I

P

P

N

N

V

T

V

V

A

A

A
x
V

G

G

V

N

P

P

C

C

R

R

V

V

I

I

R
x
K

E

K

I

I

N

E

D

E

binding acetyl coenzyme *a: N84 (= N85), A104 (= A105), W138 (= W139), G140 (= G141), G141 (≠ S142), A159 (= A160), V174 (≠ A175), K182 (≠ R183)

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
42% identity, 91% coverage: 3:187/203 of query aligns to 1:186/190 of 5u2kA

query
sites
5u2kA

M

M

P

T

M

E

T

K

E

E

R

K

I

M

R

L

A

A

G

E

K

K

L

W

F

Y

-

D

T

A

D

N

M

F

C

D

E

Q

G

Y

L

L

P

I

E

N

K

E

R

R

L

A

R

R

G

A

K

K

T

D

L

I

M

C

Y

F

E

E

F

L

N

N

H

H

S

T

H

R

P

P

S

S

E

A

V

T

E

N

K

K

R

R

E

K

S

E

L

L

I

I

K

D

E

Q

M

L

F

F

A

Q

T

T

V

T

G

T

E

D

N

N

A

V

W

S

V

I

E

S

P

I

P

P

V

F

Y

D

F

T

S

D

Y

Y

G

G

S

W

N

N

I

V

H

K

I

L

G

G

R

K

N

N

F

V

Y

Y

A

V

N

N

F

T

N

N

L

C

T

Y

I

F

V
x
M

D

D

D

G

Y

G

T

Q

V

I

T

T

I

I

G

G

D

D

N

N

V

V

L

F

I

I

A

G

P

P

N

N

V

C

T

G

L

F

S
x
Y

V

T

T
x
A

G

T

H
|
H

P

P

V

L

H

N

H

F

E

H

L

H

R

R

K

N

N

E

G

G

E

F

M

E

Y

K

S

A

F

G

P

P

I

I

T

H

I

I

G

G

N

S

N

N

V

T

W

W

I

F

G

G

S

G

H

H

V

V

V

A

I

V

N
x
L

P

P

G

G

V

V

T

T

I

I

G

G

D

E

N

G

S

S

V

V

I

I

G

G

A

A

G

G

S

S

I

V

V

V

T
|
T

K
|
K

D

D

I

I

P

P

N

H

V

S

V

L

A

A

A

V

G

G

V

N

P

P

C

C

R

K

V

V

I

V

R

R

E

K

I

I

N

D

D

N

binding coenzyme a: M90 (≠ V91), Y110 (≠ S111), A112 (≠ T113), H114 (= H115), L146 (≠ N147), T164 (= T165), K165 (= K166)

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
40% identity, 89% coverage: 7:187/203 of query aligns to 7:188/188 of 3igjC

query
sites
3igjC

E

D

R

K

I

M

R

L

A

A

G

G

K

E

L

M

F

Y

T

I

D

A

M

D

C

D

E

E

G

E

L

L

P

V

E

A

K

D

R

R

L

V

R

E

G

A

K

K

T

R

L

L

M

T

Y

R

E

L

F

Y

N

N

H

E

S

A

H

V

P

E

S

T

E

G

V

D

E

E

K

R

R

R

E

F

S

T

L

L

I

L

K

N

E

Q

M

L

F

L

A

G

T

S

V

S

G

A

E

D

-

G

N

K

A

A

W

Q

V

I

E

N

P

P

P

D

V

F

Y

R

F

C

S

D

Y

Y

G

G

S

Y

N

N

I

I

H

H

I

V

G

G

R

K

N

S

F

F

Y
x
F

A

A

N

N

F

F

N

N

L

C

T

V

I

I

V

L

D

D

D

V

Y

C

T

E

V

V

T

R

I

I

G

G

D

D

N

H

V

C

L

M

I

F

A
|
A

P

P

N

G

V

V

T

H

L

I

S
x
Y

V

T

T
x
A

G

T

H
|
H

P

P

V

L

H

H

H

P

E

V

L

E

R

R

K

N

N

S

G

G

E

K

M

E

Y

Y

S

G

F

K

P

P

I

V

T

K

I

I

G

G

N

N

V

V

W

W

I

V

G
|
G

S

G

H

G

V

A

V

I

I

I

N
|
N

P

P

G

G

V

V

T

S

I

I

G

G

D

D

N

N

S

A

V

V

I

I

G
x
A

A
x
S

G

G

S

A

I

V

V

V

T
|
T

K

K

D

D

I

V

P

P

P

N

N

N

V

V

A

V

A

G

G

G

V
x
N

P

P

C

A

R

K

V

V

I

I

R

K

E

T

I

I

N

E

D

E

binding acetyl coenzyme *a: F84 (≠ Y83), A106 (= A105), Y112 (≠ S111), A114 (≠ T113), H116 (= H115), G142 (= G141), N148 (= N147), A160 (≠ G159), S161 (≠ A160), T166 (= T165), N178 (≠ V177)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
35% identity, 91% coverage: 3:187/203 of query aligns to 1:183/183 of 3nz2C

query
sites
3nz2C

M

M

P

S

M

E

T

L

E

E

R

K

I

M

R

L

A

K

G

G

K

E

L

H

F

F

T

D

D

G

M

A

C

S

E

A

G

E

L

I

P

E

E

A

K

L

R

R

L

S

R

Q

G

A

K

G

T

R

L

L

M

K

Y

L

E

E

F

I

N

N

H

Q

S

S

H

-

P

-

S

L

E

D

V

E

E

A

K

E

R

R

E

Y

S

A

L

L

I

Q

K

R

E

E

M

L

F

F

A

G

T

H

V

L

G

G

E

H

N

K

A

S

W

C

V

V

E

Q

P

P

V

F

Y

H

F

C

S

E

Y

F

G

G

S

K

N

T

I

I

H

R

I

I

G

G

R

D

N

H

F

T

Y

F

A

I

N

N

F

M

N

N

L

V

T

V

I

M

V

L

D

D

D

G

Y

A

T

P

V

I

T

T

I

I

G

G

D

D

N

H

V

V

L

L

I

I

A

G

P

P

N

S

V

T

T

Q

L

F

S
x
Y

V

T

T

A

G

S

H
|
H

P

S

V

L

H

D

H

Y

E

R

L

R

R

R

K

Q

N

A

G

W

E

E

M

T

Y

I

S

C

F

K

P

P

I

I

T

V

I

I

G

E

N

D

V

V

W

W

I

I

G
|
G

S

G

H

N

V

V

I

I

N

N

P

Q

G

G

V

V

T

T

I

I

G

G

D

A

N

R

S

S

V

V

I

V

G
x
A

A
|
A

G
x
N

S

S

I

V

V

V

T
x
N

K

Q

D

D

I

V

P

P

N

D

V

T

V

L

A

V

A
x
G

G

G

V
x
T

P
|
P

C

A

R

R

V

I

I
x
L

R
|
R

E

S

I

L

N

K

D

D

binding acetyl coenzyme *a: Y107 (≠ S111), H111 (= H115), G137 (= G141), A155 (≠ G159), A156 (= A160), N161 (≠ T165), G171 (≠ A175), T173 (≠ V177), P174 (= P178), L178 (≠ I182), R179 (= R183)
binding magnesium ion: N157 (≠ G161), T173 (≠ V177)

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
34% identity, 92% coverage: 1:186/203 of query aligns to 2:185/185 of 3nz2J

query
sites
3nz2J

M

M

N

K

M

M

P

S

M

E

T

L

E

E

R

K

I

M

R

L

A

K

G

G

K

E

L

H

F

F

T

D

D

G

M

A

C

S

E

A

G

E

L

I

P

E

E

A

K

L

R

R

L

S

R

Q

G

A

K

G

T

R

L

L

M

K

Y

L

E

E

F

I

N

N

H

Q

S

S

H

-

P

-

S

L

E

D

V

E

E

A

K

E

R

R

E

Y

S

A

L

L

I

Q

K

R

E

E

M

L

F

F

A

G

T

H

V

L

G

G

E

H

N

K

A

S

W

C

V

V

E

Q

P

P

V

F

Y

H

F

C

S

E

Y

F

G

G

S

K

N

T

I

I

H

R

I

I

G

G

R

D

N

H

F

T

Y

F

A

I

N

N

F

M

N

N

L

V

T

V

I

M

V

L

D

D

D

G

Y

A

T

P

V

I

T

T

I

I

G

G

D

D

N

H

V

V

L

L

I

I

A
x
G

P

P

N

S

V

T

T

Q

L

F

S
x
Y

V

T

T

A

G

S

H
|
H

P

S

V

L

H

D

H

Y

E

R

L

R

R

R

K

Q

N

A

G

W

E

E

M

T

Y

I

S

C

F

K

P

P

I

I

T

V

I

I

G

E

N

D

V

V

W
|
W

I

I

G
|
G

S

G

H

N

V

V

I

I

N

N

P

Q

G

G

V

V

T

T

I

I

G

G

D

A

N

R

S

S

V

V

I

V

G
x
A

A
|
A

G

N

S

S

I

V

V

V

T
x
N

K

Q

D

D

I

V

P

P

N

D

V

T

V

L

A

V

A
x
G

G

G

V
x
T

P
|
P

C

A

R

R

V

I

I

L

R
|
R

E

S

I

L

N

K

binding acetyl coenzyme *a: G104 (≠ A105), Y110 (≠ S111), H114 (= H115), W138 (= W139), G140 (= G141), A158 (≠ G159), A159 (= A160), N164 (≠ T165), G174 (≠ A175), T176 (≠ V177), P177 (= P178), R182 (= R183)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
37% identity, 80% coverage: 26:187/203 of query aligns to 17:176/176 of 3ectA

query
sites
3ectA

R

R

L

S

R

Q

G

A

K

G

T

R

L

L

M

K

Y

L

E

E

F

I

N

N

H

Q

S

S

H

-

P

-

S

L

E

D

V

E

E

A

K

E

R

R

E

Y

S

A

L

L

I

Q

K

R

E

E

M

L

F

F

A

G

T

H

V

L

G

G

E

H

N

K

A

S

W

C

V

V

E

Q

P

P

V

F

Y

H

F

C

S

E

Y

F

G

G

S

K

N

T

I

I

H

R

I

I

G

G

R

D

N

H

F

T

Y

F

A

I

N

N

F

M

N

N

L

V

T

V

I

M

V

L

D

D

D

G

Y

A

T

P

V

I

T

T

I

I

G

G

D

D

N

H

V

V

L

L

I

I

A

G

P

P

N

S

V

T

T

Q

L

F

S

Y

V

T

T

A

G

S

H

H

P

S

V

L

H

D

H

Y

E

R

L

R

R

R

K

Q

N

A

G

W

E

E

M

T

Y

I

S

C

F

K

P

P

I

I

T

V

I

I

G

E

N

D

V

V

W

W

I

I

G

G

S

G

H

N

V

V

I

I

N

N

P

Q

G
|
G

V

V

T

T

I

I

G

G

D

A

N

R

S

S

V

V

I

V

G

A

A

A

G

N

S

S

I

V

V

V

T

N

K

Q

D
|
D

I

V

P

P

N

D

V

T

V

L

A

V

A

G

G

G

V

T

P

P

C

A

R

R

V

I

I

L

R

R

E

S

I

L

N

K

D

D

binding calcium ion: G138 (= G149), D156 (= D167)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
37% identity, 64% coverage: 55:184/203 of query aligns to 148:279/307 of A1ADJ6

query
sites
A1ADJ6

F

Y

A

V

T

R

V

I

G

H

E

K

N

N

A

S

W

K

V

I

E

K

P

G

P

D

V

I

Y

V

F

A

S

T

Y

K

G

G

S

S

N

K

I

V

H

I

I

I

G

G

R

R

N

R

-

T

F

I

Y

G

A

A

N

G

F

F

N

E

L

V

T

-

I

V

V

T

D

D

D

K

Y

C

T

N

V

V

T

T

I

I

G

G

D

H

N

D

V

C

L

M

I

I

A

A

P

R

N

D

V

V

T

I

L

L

S

R

V

A

T

S

-

D

G

G

H
|
H

P

P

V

I

-

F

-

D

-

I

H

H

H

S

E

K

L

K

R

R

K

I

N

N

G

-

E

-

M

-

Y

W

S

A

F

K

P

D

I

I

T

I

I

I

G

S

N

S

N

Y

V

V

W
|
W

I

V

G

G

S

R

H

N

V

V

V

S

I

I

N

M

P

K

G

G

V

V

T

S

I

V

G

G

D

S

N

G

S

S

V

V

I

I

G

G

A

Y

G

G

S

S

I

I

V

V

T

T

K

K

D

D

I

V

P

P

P

S

N

M

V

C

V

A

A

A

G

G

V

N

P

P

C

A

R

K

V

I

I

I

R

K

E

R

H210 (= H115) mutation to A: Loss of activity.
W234 (= W139) mutation to A: Loss of activity.

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
36% identity, 51% coverage: 96:198/203 of query aligns to 61:180/204 of 1mrlA

query
sites
1mrlA

V

L

T

K

I

I

G

G

D

K

N

F

V

C

L

S

I

I

A

G

P

P

N

G

V

V

T

T

L

I

S

I

V

M

T

N

G

G

H

-

P

-

V

A

H
x
N

H
|
H

E

-

L

-

R

R

K

M

N

D

G

G

E

S

M

T

Y

Y

S

P

F

F

-

N

-
x
L

-

F

-

G

-

N

-

G

-

W

-

E

-

K

-

H

-
x
M

-

P

-

K

-

L

-

D

-

Q

-
x
L

P

P

I

I

T

K

-

G

-

D

-

T

-

I

I

I

G

G

N

N

N

D

V

V

W

W

I

I

G

G

S

K

H

D

V

V

I

I

N

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

N

G

S

A

V

I

I

V

G

A

A

A

G

N

S

S

I

V

V

V

T

V

K

K

D

D

I

I

P

A

P

P

N

Y

V

M

V

L

A

A

A

G

G

G

V

N

P

P

C

A

R

N

V

E

I

I

R

K

E

Q

I

R

N

F

D

D

R

Q

D

D

K

T

H

I

Y

N

Y

Q

F

L

K

L

D

D

Y

I

K

K

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: N81 (≠ H118), H82 (= H119), L93 (vs. gap), M102 (vs. gap), L108 (vs. gap)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
36% identity, 51% coverage: 96:198/203 of query aligns to 61:180/203 of 3dhoA

query
sites
3dhoA

V

L

T

K

I

I

G

G

D

K

N

F

V

C

L

S

I

I

A

G

P

P

N

G

V

V

T

T

L

I

S

I

V

M

T

N

G

G

H

-

P

-

V

A

H

N

H

H

E

-

L

-

R

R

K
x
M

N

D

G

G

E

S

M

T

Y

Y

S

P

F

F

-

N

-

L

-

F

-

G

-

N

-

G

-

W

-

E

-

K

-

H

-

M

-

P

-

K

-

L

-

D

-

Q

-

L

P

P

I

I

T

K

-

G

-

D

-

T

-

I

I

I

G

G

N

N

N

D

V

V

W

W

I

I

G

G

S

K

H

D

V

V

I

I

N

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

N

G

S

A

V
x
I

I

V

G
x
A

A

A

G

N

S

S

I

V

V

V

T
x
V

K

K

D

D

I

I

P

A

P

P

N

Y

V

M

V
x
L

A

A

A
x
G

G

G

V

N

P
|
P

C

A

R

N

V

E

I
|
I

R

K

E

Q

I

R

N

F

D

D

R

Q

D

D

K

T

H

I

Y

N

Y

Q

F

L

K

L

D

D

Y

I

K

K

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (≠ K123), I139 (≠ V157), A141 (≠ G159), V147 (≠ T165), L155 (≠ V173), G157 (≠ A175), P160 (= P178), I164 (= I182)

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
36% identity, 51% coverage: 96:198/203 of query aligns to 61:180/205 of 1kk4A

query
sites
1kk4A

V

L

T

K

I

I

G

G

D

K

N

F

V

C

L

S

I
|
I

A
x
G

P

P

N

G

V

V

T

T

L

I

S

I

V

M

T

N

G

G

H

-

P

-

V

A

H

N

H

H

E

-

L

-

R

R

K

M

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binding acetyl coenzyme *a: I69 (= I104), G70 (≠ A105), K111 (≠ T133), W121 (= W139), G123 (= G141), K124 (≠ S142), A141 (≠ G159), A142 (= A160), V147 (≠ T165), K148 (= K166), L155 (≠ V173), G157 (≠ A175), G158 (= G176), P160 (= P178), I164 (= I182)

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
36% identity, 51% coverage: 96:198/203 of query aligns to 61:180/209 of P50870

query
sites
P50870

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H82 (= H119) mutation to A: 105-fold decrease in activity.

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
36% identity, 51% coverage: 96:198/203 of query aligns to 61:180/206 of 1khrA

query
sites
1khrA

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binding coenzyme a: H82 (= H119), M84 (≠ K123), W121 (= W139), A141 (≠ G159), A142 (= A160), V147 (≠ T165), L155 (≠ V173), G157 (≠ A175), P160 (= P178), I164 (= I182)
binding virginiamycin m1: A80 (≠ V117), N81 (≠ H118), H82 (= H119), L93 (vs. gap)

Sites not aligning to the query:

binding virginiamycin m1: 37, 39, 54

7s45A Crystal structure of an n-acetyltransferase, c80t mutant, from helicobacter pullorum in the presence of acetyl coenzyme a and dtdp (see paper)
34% identity, 58% coverage: 56:172/203 of query aligns to 31:135/141 of 7s45A

query
sites
7s45A

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binding acetyl coenzyme *a: W64 (≠ V91), T82 (≠ S111), N83 (≠ V112), D84 (≠ T113), K85 (≠ G114), Y86 (≠ H115), P87 (= P116), L110 (≠ N147), T128 (= T165), K129 (= K166)
binding thymidine-5'-diphosphate: F44 (≠ Y69), E46 (≠ S71)

7uukC Crystal structure of aminoglycoside resistance enzyme apma, complex with tobramycin (see paper)
45% identity, 26% coverage: 131:183/203 of query aligns to 178:232/276 of 7uukC

query
sites
7uukC

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Sites not aligning to the query:

binding tobramycin: 83, 87, 104, 105, 115, 137, 146, 147

7uumA Crystal structure of aminoglycoside resistance enzyme apma, complex with paromomycin and coenzyme a (see paper)
45% identity, 26% coverage: 131:183/203 of query aligns to 178:232/274 of 7uumA

query
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7uumA

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binding coenzyme a: F192 (≠ V145), P227 (= P178)

Sites not aligning to the query:

binding coenzyme a: 134, 136, 169
binding paromomycin: 114, 115, 137, 146, 147

7uulA Crystal structure of aminoglycoside resistance enzyme apma, complex with kanamycin b and coenzyme a (see paper)
45% identity, 26% coverage: 131:183/203 of query aligns to 177:231/274 of 7uulA

query
sites
7uulA

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binding coenzyme a: Y185 (≠ W139), G187 (= G141), A188 (≠ S142), F191 (≠ V145), S208 (≠ A160), L213 (≠ T165), V223 (≠ A175), K230 (≠ I182), R231 (= R183)

Sites not aligning to the query:

Query Sequence

>14480 FitnessBrowser__Keio:14480
MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVEKRESLIKEMFATVGE
NAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLIAPNVTLSVTGHPVHHE
LRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSIVTKDIPPNVVAAGVPCR
VIREINDRDKHYYFKDYKVESSV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory